LOCUS       DAPCMT010000001        68373 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPCMT010000001 DAPCMT010000000
VERSION     DAPCMT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 68373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.001"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            51..932
                     /locus_tag="RD743_000001"
     CDS             51..932
                     /locus_tag="RD743_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0561865.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            945..2555
                     /locus_tag="RD743_000002"
     CDS             945..2555
                     /locus_tag="RD743_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDU0561866.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2632..3291)
                     /locus_tag="RD743_000003"
     CDS             complement(2632..3291)
                     /locus_tag="RD743_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDU0561867.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3574..4239)
                     /locus_tag="RD743_000004"
     CDS             complement(3574..4239)
                     /locus_tag="RD743_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDU0561868.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4386..4991)
                     /gene="dhaL"
                     /locus_tag="RD743_000005"
     CDS             complement(4386..4991)
                     /gene="dhaL"
                     /locus_tag="RD743_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDU0561869.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5019..6029)
                     /gene="dhaK"
                     /locus_tag="RD743_000006"
     CDS             complement(5019..6029)
                     /gene="dhaK"
                     /locus_tag="RD743_000006"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDU0561870.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6004..6450)
                     /gene="dhaM"
                     /locus_tag="RD743_000007"
     CDS             complement(6004..6450)
                     /gene="dhaM"
                     /locus_tag="RD743_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDU0561871.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6553..6873
                     /locus_tag="RD743_000008"
     CDS             6553..6873
                     /locus_tag="RD743_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDU0561872.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6985..7854)
                     /locus_tag="RD743_000009"
     CDS             complement(6985..7854)
                     /locus_tag="RD743_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDU0561873.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7950..9253)
                     /locus_tag="RD743_000010"
                     /pseudo
     CDS             complement(7950..9253)
                     /locus_tag="RD743_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9250..9759)
                     /locus_tag="RD743_000011"
     CDS             complement(9250..9759)
                     /locus_tag="RD743_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0561874.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9934..12069)
                     /locus_tag="RD743_000012"
     CDS             complement(9934..12069)
                     /locus_tag="RD743_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0561875.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12152..13807)
                     /locus_tag="RD743_000013"
     CDS             complement(12152..13807)
                     /locus_tag="RD743_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDU0561876.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13809..14123)
                     /locus_tag="RD743_000014"
     CDS             complement(13809..14123)
                     /locus_tag="RD743_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDU0561877.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14293..14553
                     /locus_tag="RD743_000015"
     CDS             14293..14553
                     /locus_tag="RD743_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDU0561878.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14567..14881
                     /locus_tag="RD743_000016"
     CDS             14567..14881
                     /locus_tag="RD743_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDU0561879.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15611..16165)
                     /locus_tag="RD743_000017"
     CDS             complement(15611..16165)
                     /locus_tag="RD743_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDU0561880.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16291..18219)
                     /locus_tag="RD743_000018"
     CDS             complement(16291..18219)
                     /locus_tag="RD743_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDU0561881.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18525..19241)
                     /locus_tag="RD743_000019"
     CDS             complement(18525..19241)
                     /locus_tag="RD743_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDU0561882.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19242..21065)
                     /gene="glpO"
                     /locus_tag="RD743_000020"
     CDS             complement(19242..21065)
                     /gene="glpO"
                     /locus_tag="RD743_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDU0561883.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21079..22575)
                     /gene="glpK"
                     /locus_tag="RD743_000021"
     CDS             complement(21079..22575)
                     /gene="glpK"
                     /locus_tag="RD743_000021"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDU0561884.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22711..24171)
                     /locus_tag="RD743_000022"
     CDS             complement(22711..24171)
                     /locus_tag="RD743_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0561885.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24161..25456)
                     /locus_tag="RD743_000023"
     CDS             complement(24161..25456)
                     /locus_tag="RD743_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDU0561886.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25788..26465)
                     /locus_tag="RD743_000024"
     CDS             complement(25788..26465)
                     /locus_tag="RD743_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDU0561887.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26495..27586)
                     /locus_tag="RD743_000025"
     CDS             complement(26495..27586)
                     /locus_tag="RD743_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDU0561888.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27687..28121)
                     /locus_tag="RD743_000026"
     CDS             complement(27687..28121)
                     /locus_tag="RD743_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0561889.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28705..30162)
                     /gene="ascB"
                     /locus_tag="RD743_000027"
     CDS             complement(28705..30162)
                     /gene="ascB"
                     /locus_tag="RD743_000027"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDU0561890.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30159..32054)
                     /locus_tag="RD743_000028"
     CDS             complement(30159..32054)
                     /locus_tag="RD743_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0561891.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32112..33887)
                     /locus_tag="RD743_000029"
     CDS             complement(32112..33887)
                     /locus_tag="RD743_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDU0561892.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33937..35433)
                     /locus_tag="RD743_000030"
     CDS             complement(33937..35433)
                     /locus_tag="RD743_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0561893.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35627..37249)
                     /locus_tag="RD743_000031"
     CDS             complement(35627..37249)
                     /locus_tag="RD743_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDU0561894.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37698..39011)
                     /locus_tag="RD743_000032"
     CDS             complement(37698..39011)
                     /locus_tag="RD743_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDU0561895.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39256..39480)
                     /locus_tag="RD743_000033"
     CDS             complement(39256..39480)
                     /locus_tag="RD743_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0561896.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39500..39922)
                     /locus_tag="RD743_000034"
     CDS             complement(39500..39922)
                     /locus_tag="RD743_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDU0561897.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40066..40776)
                     /locus_tag="RD743_000035"
     CDS             complement(40066..40776)
                     /locus_tag="RD743_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0561898.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40794..41291)
                     /locus_tag="RD743_000036"
     CDS             complement(40794..41291)
                     /locus_tag="RD743_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0561899.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41328..41780)
                     /locus_tag="RD743_000037"
     CDS             complement(41328..41780)
                     /locus_tag="RD743_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0561900.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41915..42520)
                     /locus_tag="RD743_000038"
     CDS             complement(41915..42520)
                     /locus_tag="RD743_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDU0561901.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42970..43590)
                     /locus_tag="RD743_000039"
     CDS             complement(42970..43590)
                     /locus_tag="RD743_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0561902.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43592..44461)
                     /locus_tag="RD743_000040"
     CDS             complement(43592..44461)
                     /locus_tag="RD743_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDU0561903.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44484..44891)
                     /locus_tag="RD743_000041"
     CDS             complement(44484..44891)
                     /locus_tag="RD743_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDU0561904.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44910..46199)
                     /locus_tag="RD743_000042"
     CDS             complement(44910..46199)
                     /locus_tag="RD743_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0561905.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46226..46528)
                     /locus_tag="RD743_000043"
     CDS             complement(46226..46528)
                     /locus_tag="RD743_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0561906.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46553..48646)
                     /locus_tag="RD743_000044"
     CDS             complement(46553..48646)
                     /locus_tag="RD743_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDU0561907.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49135..49899)
                     /locus_tag="RD743_000045"
     CDS             complement(49135..49899)
                     /locus_tag="RD743_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDU0561908.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(50010..50879)
                     /gene="mdcB"
                     /locus_tag="RD743_000046"
     CDS             complement(50010..50879)
                     /gene="mdcB"
                     /locus_tag="RD743_000046"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDU0561909.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50904..51980)
                     /locus_tag="RD743_000047"
     CDS             complement(50904..51980)
                     /locus_tag="RD743_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDU0561910.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(52004..52597)
                     /locus_tag="RD743_000048"
     CDS             complement(52004..52597)
                     /locus_tag="RD743_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDU0561911.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52601..54277)
                     /gene="mdcD"
                     /locus_tag="RD743_000049"
     CDS             complement(52601..54277)
                     /gene="mdcD"
                     /locus_tag="RD743_000049"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDU0561912.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54267..54566)
                     /locus_tag="RD743_000050"
     CDS             complement(54267..54566)
                     /locus_tag="RD743_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDU0561913.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54668..56314)
                     /gene="mdcA"
                     /locus_tag="RD743_000051"
     CDS             complement(54668..56314)
                     /gene="mdcA"
                     /locus_tag="RD743_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDU0561914.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56321..57232)
                     /locus_tag="RD743_000052"
     CDS             complement(56321..57232)
                     /locus_tag="RD743_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDU0561915.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57352..58209
                     /locus_tag="RD743_000053"
     CDS             57352..58209
                     /locus_tag="RD743_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0561916.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58396..58758)
                     /locus_tag="RD743_000054"
     CDS             complement(58396..58758)
                     /locus_tag="RD743_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDU0561917.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            59104..60543
                     /locus_tag="RD743_000055"
     CDS             59104..60543
                     /locus_tag="RD743_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0561918.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60550..63102)
                     /locus_tag="RD743_000056"
     CDS             complement(60550..63102)
                     /locus_tag="RD743_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDU0561919.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63216..64547)
                     /locus_tag="RD743_000057"
     CDS             complement(63216..64547)
                     /locus_tag="RD743_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0561920.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64513..65991)
                     /locus_tag="RD743_000058"
     CDS             complement(64513..65991)
                     /locus_tag="RD743_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0561921.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66296..68131)
                     /gene="tdc"
                     /locus_tag="RD743_000059"
     CDS             complement(66296..68131)
                     /gene="tdc"
                     /locus_tag="RD743_000059"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDU0561922.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19445 a        14585 c        11702 g        22641 t
ORIGIN      
        1 ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa
       61 tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct
      121 ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat
      181 ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt
      241 ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag
      301 gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc
      361 ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc
      421 ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg
      481 ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt
      541 tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc
      601 tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac
      661 gcgaaggaac agtcgtagaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga
      721 ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg
      781 actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga
      841 ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg
      901 aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt
      961 cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat
     1021 ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt
     1081 ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg
     1141 tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg
     1201 aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc
     1261 aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac
     1321 gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa
     1381 tatcgatcag ctgtacgatg atttttttcc aagcagattc gatcactgca gaaggtgctt
     1441 ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg
     1501 ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg
     1561 gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc
     1621 ctttaggctg gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt
     1681 atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag
     1741 atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt
     1801 tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca
     1861 cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag
     1921 aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact
     1981 ttacaagcac gatcatggtc gtcttgattt gtctagctgc tattttacca attgctagca
     2041 tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa
     2101 tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt gctatattgg
     2161 caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga
     2221 aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag
     2281 tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatgggt aaacttgctt
     2341 atatctactt agcttactcc tttgcactca attactttgg cggaattttg gatttaccag
     2401 aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg
     2461 atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta
     2521 ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc
     2581 tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc
     2641 ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt
     2701 ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc
     2761 aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc
     2821 aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg
     2881 ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata
     2941 atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag
     3001 tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc
     3061 gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt
     3121 catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc
     3181 tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac
     3241 taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc
     3301 tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca actgataatt
     3361 ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct
     3421 ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt
     3481 ttttaaatat ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa
     3541 ggtttgtttt tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt
     3601 ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat
     3661 ttcttgcgga tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt
     3721 caactcttct tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag
     3781 ttcatgttct ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc
     3841 tttcagtatc gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt
     3901 ataatgttgg tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca
     3961 ggcattctcg tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt
     4021 ccagccattc gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag
     4081 tgcagctgtc tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt
     4141 gatttgttca atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg
     4201 tcgatcaaac aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat
     4261 cttttgtatt ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc
     4321 tgcatcaagt cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat
     4381 tgaacttaag gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca
     4441 gctcctgggt cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta
     4501 gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct
     4561 gccttcaatg cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct
     4621 gcctttcctc ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct
     4681 tgagaatcaa tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg
     4741 tataacggac cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat
     4801 gtatcgctga ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct
     4861 cttcccatgt tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat
     4921 tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg
     4981 gttagtttca tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca
     5041 ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga
     5101 ccttgcatat ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct
     5161 ctttcttcca acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc
     5221 ccgcccattc cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa
     5281 atcttagtaa caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag
     5341 cctggctcac cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct
     5401 ggtttcccta cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc
     5461 tgtacgacct tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca
     5521 tcgcccacga ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac
     5581 gtactatttt caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc
     5641 atagcagcta attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg
     5701 atcagcaaaa ctccttttcc actatccgct tccttaatag ccgtctgaat ctgatctgga
     5761 gttggagaag taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac
     5821 ccggcatggg atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct
     5881 ttctgattct tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt
     5941 tttactaatc cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc
     6001 aatttattcc catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc
     6061 atctgttata ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac
     6121 cagatgataa cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa
     6181 taccgcgcta ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac
     6241 tgcttctacg attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa
     6301 aatttgcacc tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac
     6361 tttttgacta tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac
     6421 atagttgcta acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt
     6481 ctaattttgg gaagatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag
     6541 aggagtgatc gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct
     6601 tataaaggga tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag
     6661 gcggggttgc gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt
     6721 gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg
     6781 atcattggcg taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc
     6841 acatgtagtg gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa
     6901 agagagatgg gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta
     6961 ctctaccttc ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc
     7021 gactaacaca cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt
     7081 tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt
     7141 gcttccagag gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa
     7201 actagctgct aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct
     7261 gatctttccg tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt
     7321 tccagaagct gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat
     7381 cttcccgtca agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt
     7441 ctcaagttgt gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat
     7501 tgctgcttgg tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt
     7561 tcttttattt tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat
     7621 tgatgtatct tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt
     7681 catttctgtt tccgaggcac cgttttcagc accagaagag cttatagttg ttgaaggttc
     7741 tgtttgagca ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga
     7801 aacagcaatt ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac
     7861 tactttttaa ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca
     7921 ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat
     7981 gtcccgcttc cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt
     8041 atcgcgttca ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat
     8101 tttcttcgtc gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt
     8161 tcaaagcaga tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct
     8221 tgatatacat aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt
     8281 gaccagacaa tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa
     8341 ggatggagca tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa
     8401 cgttttcttt gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc
     8461 tcaggagcaa aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct
     8521 tctgtcgctc gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc
     8581 gtatttgctc cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg
     8641 atgcccacga tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt
     8701 tggacatcca tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa
     8761 ttcacatacc taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc
     8821 aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt
     8881 tcgatccgaa ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt
     8941 gtttggtccg ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc
     9001 gtcatcaaat actcgattgt atcttcgatc acaatttctt cattatgggc tggcaccatg
     9061 atcgtgatga acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg
     9121 tataaaaaca cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa
     9181 gaaacgagaa aaaatacata atagacaaac aagtatctaa gaataaatta cctgtatggg
     9241 tgattgagat catttcagtt ccaccccatt ttctttatac aaatctgcaa tgaatgtcgt
     9301 atctagattt ttctcttcgg agacagaata aaaacggacc gtttctctct cttcttttgt
     9361 tccaaaacgt tccgtataca cttcttcaag taattcttgt ctttttttca atttttcatc
     9421 ttcatattca aaagatttat ttactttttt gtatagattt cgattgttcc acctagtcaa
     9481 tacaaatgag gtaatcagga aactgaaaaa aatcaataca aaaaagccga ttaagaaata
     9541 cagcattttc ttttctgata aaaaagcgta caaggaaaaa tccttggctc tttctggtct
     9601 cgttaatggg aaaaagaccc atataaaagg aagaacaacg atcatccatc ctaaaagagc
     9661 cactaacgtt tgccaaatct ttaaccagta attacctttt gaaaaatact tatctttcac
     9721 caaaattgat gcaacttctt ctcttgttac ttttttcata tcctcacctc acgttttttt
     9781 gttacgacgt aggagtttac catacatttc atttcttttc acataaagtg ttcctaaatt
     9841 tttcctaaga aaaagaacaa aacatttctc atcagcaaat gaaaaatatt ttgttctttt
     9901 tatcagttgt tctttttatt ttttatctct taattatgtt gctctttcca atacgacaat
     9961 gcttttcatt cctctagata tcgccgtgta catttgtctg cgtcttcttt gctcatctcc
    10021 atcgttttca aatggatata agattacatg atcaaattcc agtccttttg ctagactcaa
    10081 aggaatgatt tggcagtttt ctaatccata tgaatttttt ttaatcgatt cttgtagcgt
    10141 ttgggtttcc tcttctgttt gggtgataac tgccgctttt tctcctttta gagaaaccaa
    10201 aatctcagct agtattttca acaattcttc agaggatcga aaagagcggt attcgggttc
    10261 ttttccttgc ggtcggatcg atacgatgtc gatcttctct tgatccacag cataggtttt
    10321 gaaaagctct gtgattgctc cgctagaacg gtaactattt ctaagatcat accgatggat
    10381 cggcagatgg tagtcgtcga aacaacgcat aatatcagca aaagtagtgg aagaattaaa
    10441 gattgcctga ttctcgtcac ctactaaagt aaagtgagtt tttggaaata attcgatcaa
    10501 tagacctaat tgtgccaggg tataatcttg tacttcatca atcaatagat agcgaaatgc
    10561 tggtacattt accttttcaa caagtaaatg acgaatagct aatataatga ctgcttcatc
    10621 aacagtacgt gtgggttgcc aagcataagc tgagccatat cgtttttcat aaattctttc
    10681 aaacaactga tgctcatcca cccatgccat ttcttcgatt tgtctggtta tttttcgata
    10741 ttttttttgt aaaagtttcc ttgcataagc tacaatactt tgttccgaat catcagaaat
    10801 caatttaccg aataattcca actgcctatc ttctgtcaac gagagtacct gattctgcac
    10861 agcaggactt tttgcttgtt tcaaaagatg attcttccat tcgctcatca gtgccttttt
    10921 cgttgcttga agcctatcga tcaatttagg atgagcagga actttctcat atattttttc
    10981 gatcgttttt ttagaaagga ctgtttttcc atttttagta agtccacgga taaaattcgg
    11041 atgatctaaa aacaaaggat ctgattgttt tagattatcg ataaactttt tgctacgcaa
    11101 acgttctgtc tgctccgata cattttctcc cgtgatacgt ttgaaataag cctcttcacc
    11161 ttctatttcc tctgctgaaa gctgtgaaac cagctgtatg atggttagat tcagtggatt
    11221 tctttcacct aatgatggta ggacattcga aatatagtcg ataaaccgat gattaggtga
    11281 tagaatcagc aagtcatcgg atgtcatcgt atctcgatat aaatagagca gatacgcaat
    11341 acgctgcatg atagcagagg ttttaccact tcctgctacg ccatttacca agatattttg
    11401 acttttcaca tcccgaatga ttgtgttttg ttccgattgg atcgtagcag tgatagcctt
    11461 catttcattg gtctcatctt ggcctaagac ttctaataat acgtcatctt gaatagctac
    11521 agacgtatca aaaaacttga ccaaacgatc tctttccaaa agaaattgtc gtctttcttt
    11581 aataaccgta tcgattttat tttgacgaac cgtataagaa gatggaccaa tcatgttatt
    11641 gtaaaaaagt tcggcaattg gtgaacgcca atcataaaca agattttcat ctgcatcatc
    11701 tgtgaaattt gctgtaccga tataaaagga ttcttttggc tcattatcca gaaagtctac
    11761 aacgactttt ccaaagtagg gacttttcaa taaacgttcg atctttgcta gctgattttc
    11821 tgcgtttttc tgtttgatat tcatctgatc gatttctcta tttttcattt caatcatcga
    11881 aaaagtatcc aggttatcta agtaactgtc aaaattcaat cgaagctcgc cagccatttc
    11941 tttcaacgac tggtttcctt ctatttttgc cgtatccatc gattcccgca gttcgttttt
    12001 tttatcaatt agcttttgat acacgttctt cagatatgct tcttctaact gtttttgtgt
    12061 gttgaccata cacattctcc tcttttttta aagtggtgga cccgattagt ttacctcttt
    12121 taagagtcga atgcaagctt tttaacgagt atcatttgtt aggatatatc agttcttcag
    12181 gtcggacagt ttgatataaa gaaaatgcac catcaaggtt ttttacgtgg tagccagact
    12241 gcttcaagat tctttccgct agatagctac gtaatccgct atgacagctg acgatatatt
    12301 cttgttggct gtctaattca tttaatcggt cccgaagttc attcaaaggg atatgtttgg
    12361 cttccttgaa tttgcccttt tccaattctt ctgctgttcg cacatctaat aaaatttttc
    12421 cttttgctag ttctgtcggc aattcatgcc attgtacggt ttcactaagc ccttctgcaa
    12481 tgttcattgc tgcatatcct agcatgttta ctggatcttt ggctgagcca aatggcggtg
    12541 catacgtcaa ttctaattcc ggtaaatcga agatcgtcaa atgccctttg attgctgttg
    12601 ctaaaatatc gatccgttta tccacacctt ttgccccaac accttgtgca ccataaatct
    12661 cgccagtctc tggatggaag atcaacttca gtaaaatatc tgttgcttct ggataatagc
    12721 ttgcatgatc ttttcctgat acatgaatga cttcaaaaga cagttcgttt tctttggctg
    12781 ttctttcact taaaccggtg gatgcggcag tcattccaaa cgttcgtata attgctgttc
    12841 caataccgcc ttgatttctt cgagaaattc cggaaatcgt gtccgcaact tgtctacctt
    12901 gacgattggc tggtgaagca agagaaataa gtgcatcttt tccagtgatt tgctgtttta
    12961 caacgatagc atcccccacc gcataaatat caggaatatt cgtttgataa cgttcgtcta
    13021 ctaggatacc cccgcgtatg cctaattcga ttccagcggt cactgctagc tggttatcag
    13081 gacgaacacc aatggataaa ataactaaat cagcagggat ggtacgccca tcatccagtc
    13141 gcaatcgttt cccgtggtct tcaaatccag cgacagcatg agaagtaatc acttctactc
    13201 cttgatgaga caattctttt tctataaaag ctgccatttc ttcatccagt gttggaagaa
    13261 tatgtggtgc ttgttcgatc accatgactt ctaaacctct tctttttagg ttttctgcca
    13321 tttccaatcc gatgaatcct gcaccaacga tcacggcacg ctttggctgt ttttctaatg
    13381 catgcatcac tttatcgata tctggtacat ttctaataga aaaaacattg tcggcttctg
    13441 ttatccctgg aatcgatgga acaactggtt ttgcaccagg agataaaacc aatgcatcgt
    13501 actgttcaat gtgtttttga ccctcatgta cgacttctac cgttttgttt tcgggaaaga
    13561 tggctgtcac ttcatgattt ggacgcacat ctaaacaaaa acgagcggat aatgattctg
    13621 gcgtttggac aagaagattt tcccgttcac tgatttctcc cgaaagataa taaggcaagc
    13681 cgcaatttgc aaaagaaaca tacggtcctt tttcaaaaac aacgatttct gcatcttcca
    13741 ttaatcggcg aagccgtgta gcagcagaca ttccacctgc tacacctccg acaataacga
    13801 ttttcatctt atttccctcc tactgttcga tcaaaccatt gattcatgcc accacgtaca
    13861 ttcactacat cataaccaga tttctttaat tctttagctg cttgtttact cctcatccct
    13921 gattgacaaa tcacatagac ggtcttttct ttgtctccat catatctgtt tatactttgt
    13981 aatggaacat ttttagctcc ttttatatgc ccgccccggt attctgcagg tgttctaaca
    14041 tcaagtaacg ctggattttc caaaagcttt tcctttaatt ctgggactgt tatcgatttt
    14101 attgattgaa atagcaaatt cattttctgc acctcattta catttttata aatacctcat
    14161 ggggtatatt aaacaggagc tttattaata tgtcaaccag aatgactctt catttttttc
    14221 actacttttt atatttgcat aaaataccga taaggggtat tatctaatta taaaaataga
    14281 ggagaatgtg ttatggagat tgataataaa aatatcctga accgtttgaa acgtacagag
    14341 ggacaaattc gtggtatcca aaaaatgatc gaagacgaaa aagaatgtat ggatgtcatc
    14401 actcaattaa gcgctgttcg ttctagtatt gatcgcgtaa tgggcatgat cgttgcagat
    14461 aatctgaaaa attgctttga gaaacctgat agtaatccag aagaacaagc ggctaaacta
    14521 gaacaagcga tcaagatgat tattaaaaag taaaggaatg atttcaatga aagtcgtttt
    14581 ccatattgat gaattagaaa aatggtcaga aacagggaaa aacgtaaaaa acctgataaa
    14641 agcttctcca gaaacgacga ttgtcgtatc tgtaaatggc atcgcgatta ccggttatct
    14701 agactctgcc aatgcagaat tcttagattt gcaaggagtc gtttttcacg cttgcgccaa
    14761 tgctatgcgt gctaatcata tttcagaaag ttctttacct gaacaagtaa tcgttgtacc
    14821 agcaggtgtc cttgatttag ttgaactgca atcccaaggt tatgcttata tcaagcctta
    14881 gtcagtagag gttgcgacag aagtgatcag cttcaagaaa tttcggctta tttttcgaag
    14941 aagctacttc tggaaaaccg tgaatcaaag gttgtgaaaa cgctgtcctg catcaagtaa
    15001 taagaacagc caaacaaaaa tagcttctca tatttttcgt ttggttgggc ttattaccgc
    15061 agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgg tttgacctaa
    15121 gtattagaga aaaaaacggg aaaatagctc ctcatatttt gccgtttttt gagctaatac
    15181 cgaaggtcag ctgattgaac accgtttaag gaagagattg tgaaaaagtc gcttagtttc
    15241 gagtattaag aaaaattttg tgaaaatgga tttctacatt tttgcataaa tttggcttaa
    15301 taccgagaaa ctggcttttg aataccgttt aaggaagagg ttgtaaaatc agcggtttga
    15361 cctgagtatt agagaaacaa acggaaaaat agctgctcat attttgccgt tttttgagct
    15421 aataccgaag gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc taccatttaa
    15481 ctccgaatca caagaaataa tttcaaaaac aacttctcct gtttttggtt tggttggctt
    15541 attatcgaga agtagctact gggagtctat ttatcaaaaa aaggaatgcg acataggtca
    15601 cacccctttt ttattttctt tgaaacatct ttcggtactc gacagcatac aagacaaaag
    15661 attgtaaaag accgtaagtg atgacggcaa aaatgactct cacccacagc gttggtaatt
    15721 ctgggaaaag cagcaaaagg atcatgccaa catagatgac agcagtcgct actttgccat
    15781 accacttcgc tccacttaat ttgatatttt tagatagccc gattaatcca gctacaaaca
    15841 taaagccttc ttttatcaag aatagaccaa acaacagcca aagccacggt cgataccatg
    15901 ccatagacaa aatcagaaaa agttgtgtca atttatctgc aaacggatca agcaccttcc
    15961 ctaagtctgt tacctgatta tatctccgag caatgacccc gtctagataa tcagacaaac
    16021 ctgagacaac aatgaccgct cctgctatat aaaatgactg tctcagcaca aaataaagat
    16081 aaatcggcag caaaaaaata cggaatatcg ataaaagatt cggaatcgtt tgtatttctt
    16141 tacgccagtc tgtgcttaat ttcattttat cgccctacct cctcagcact cttttctttt
    16201 tattttagct tacacgggga ataaaaccaa tgatttcact gtttttattt ttctttacag
    16261 ccactgatat tatgagctaa tttgagataa tcacttgctg ttttcatcgt ttactcttct
    16321 ttcttcctgt cgtttgcgca tttcttttac tagtacttta tgagcttttt ttgatcctcg
    16381 ctctaggtca cgctgccgtt tacgtgtttt ataatcgata aacagactgt ctaaatcgta
    16441 acctgtcggg tacagatctt ctgctgaacg caccagacga acacgctgac gaggcacgtg
    16501 gattattttt ttgtctaaat atacatcaat cgtatcttca cctatatctt cataaactaa
    16561 cgcttctttt tggtattcat tgacaaatat ccgatcacct tttgcaaaga gctggcgttc
    16621 ttttttattc tccttcctat ttgtaactgc tgtcttagga agaaattctt ttttctgtgt
    16681 ccgataggct ttttccgcca agtaacgctc tgcttgatgg atcaatttca tcgacatatc
    16741 cattttatga gcaatccaca aagcttggct ttctcctgtt tctccaaccc gcaactgata
    16801 tttaggacga agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc
    16861 tccggcgaat ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc
    16921 atacatagat tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact
    16981 gccaatctca tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat
    17041 gttttgcata tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac
    17101 atcgacgaaa atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat
    17161 accgaacatc gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc
    17221 ggcatttgct ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg
    17281 taccgcatgc tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt
    17341 attgattcta ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat
    17401 atctaacacc gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc
    17461 accagtcaat gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg
    17521 atagtgttca ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt
    17581 tgattcatcg ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt
    17641 atagtattcg cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg
    17701 acgcaagaat ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg
    17761 tactttacgc aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc
    17821 aatcaactct tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg
    17881 tgtctggtat tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc
    17941 cgcatattcg atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag
    18001 tgcatcgatc ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg
    18061 tgcttctctt gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat
    18121 atcgctgatt cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat
    18181 ccgatcaaat tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga
    18241 gatacctgtc ccaaaataaa taagggccta gagataaata aaaggataga acacagctta
    18301 aaaaagtcat gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt
    18361 ttcataagca caccaaataa actttgttta ttggctattc gacttactga ttagatacca
    18421 gctacactca caaaacatac cccattcttc taggtcttat ttattttcca cttaactata
    18481 gcaagacaca ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa
    18541 tagacaccta ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat
    18601 gcccaatctg aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca
    18661 aagtcccgag cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct
    18721 aagatcaatc cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa
    18781 gctagcaacc caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga
    18841 taattccgta tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt
    18901 gcattccaat gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa
    18961 acttgcgcaa tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata
    19021 gctagagaga ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc
    19081 attgttacag cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta
    19141 cttttgttta aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc
    19201 ccaataaact ctcccatgat ttgagtcata tcactgttca tttatttctc cccctttagt
    19261 tcccgcagat ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc
    19321 tcttctgtcc attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca
    19381 agctgatctc gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc
    19441 aaaaccattt cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca
    19501 ggataaggtg ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa
    19561 tcagcgatat aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat
    19621 tctgtcactg tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt
    19681 ttagagtcga tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat
    19741 cgcaaagctc cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc
    19801 aaaccatctg gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca
    19861 tcacggctgt tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt
    19921 tccttatatt cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca
    19981 ttatctccac cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc
    20041 cagcttgctt cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca
    20101 tccaatagat aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa
    20161 tccgtatctg tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc
    20221 ccatcttgct ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct
    20281 acaactaaat ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc
    20341 aagtttcgta ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt
    20401 tcgatcactt catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca
    20461 tataaaaagc caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt
    20521 ttgatattat caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg
    20581 ccttttagcc cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc
    20641 gtataattct catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct
    20701 actttgaccg agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc
    20761 attggatctg gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact
    20821 gtgtcagcta ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt
    20881 ttggtcgagc gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg
    20941 attcctgatg ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc
    21001 aacagatcca attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa
    21061 aacatatgga tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc
    21121 gttgtaaagt ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc
    21181 ataaaattcg cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa
    21241 tgctgcccct agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat
    21301 atctgtttgg aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac
    21361 aggaatcttg attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc
    21421 aatcgactgt aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa
    21481 catagcccct cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac
    21541 aacataaacc tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt
    21601 ttgcaacatt tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact
    21661 tccttctaaa gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt
    21721 atttttggat aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata
    21781 agtattcttg accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc
    21841 tcctgccatc cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc
    21901 ataaacttct gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc
    21961 taaaatctcc tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt
    22021 cgagtaatca gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat
    22081 cgttccaaac atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag
    22141 aatccagcgt atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg
    22201 gaaaaattct tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga
    22261 ttgccaaaca actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt
    22321 ttcacgctgg ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat
    22381 gaacgctccg gcaataactg actgtacaga attccagatt tcatttgcat catgttccac
    22441 ccaactttct tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc
    22501 tttcttgtca aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata
    22561 cgttgattct gtcattgtga ttccctccat tatatgcaaa cgctttcttt aactactctt
    22621 atcatactac tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg
    22681 acagtttttt attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat
    22741 tcttcttttc aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca
    22801 ctgttttttg tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata
    22861 acttccaatc tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc
    22921 aatacgataa atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata
    22981 aaaatcgatc atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc
    23041 ttcagctctt ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg
    23101 tgttaaggaa tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac
    23161 ttcgatcttt ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac
    23221 tgccttttct tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg
    23281 aatgtaagta tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata
    23341 aaatatttct tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc
    23401 gtatacattc agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc
    23461 ttgataaagc cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact
    23521 tttttcattc aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc
    23581 tgatgccgta aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc
    23641 tggtgtattt tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata
    23701 ataaaaatat cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc
    23761 aaattcctct aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat
    23821 attaaacgct gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag
    23881 catcttaaac ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc
    23941 ttcgtccaac acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat
    24001 cattcggcca atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg
    24061 gacatgctct ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa
    24121 aatcaggatc tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat
    24181 tcgccacctt tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat
    24241 gttcttctaa ctgttcaaac gtccatcctt cttctactgc aattgtcagt tggtcaagaa
    24301 ggtggaacat ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa
    24361 atagaaattt gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc
    24421 ctgtttcaat tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta
    24481 agaaatagcc aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg
    24541 agccactaat tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa
    24601 catacatttt ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa
    24661 cgtctttttg agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg
    24721 ttaattgttc ttcccacatc tgattgtcgg ggcgagaatg attagccagc aaaacaagat
    24781 catagttctc ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca
    24841 caacttgttc ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt
    24901 ctgtcatttc aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga
    24961 tttgtttttt tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa
    25021 taattgcaat tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct
    25081 ttttaactgg gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac
    25141 cagtcgctag aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt
    25201 tttctttcaa tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca
    25261 gttcttcttt cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca
    25321 aattgaaccc gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac
    25381 ttgttggcat caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga
    25441 caattacaat gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca
    25501 atcagaaaac acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag
    25561 aatcaaccct ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac
    25621 ctaattgaag agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa
    25681 aaaactcgcc ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag
    25741 ttttagcgtg tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat
    25801 gcttcaacaa ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc
    25861 cgcccatcaa tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt
    25921 tctttcaaag catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct
    25981 tcttcattac tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct
    26041 gtaactggca acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga
    26101 tagatttgtg aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg
    26161 tggccaccag cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag
    26221 gtatttccca aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca
    26281 tccatattca aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa
    26341 tctgcatcga tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac
    26401 aaacctgtag gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact
    26461 ttcattttcc catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat
    26521 tatataatgg gcttggcaaa taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa
    26581 attcatcctc acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct
    26641 gtccaattgc tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga
    26701 tcccaccgac accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa
    26761 tcgatttatc gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat
    26821 gcagataatc tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac
    26881 ggatacctgg tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt
    26941 tagcatattg ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac
    27001 gattttccaa gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt
    27061 gttggatcaa atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg
    27121 ctctacgtgt ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg
    27181 ctcgtggtgt ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt
    27241 ctccagataa agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt
    27301 ttgcgccttc tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat
    27361 gagcgatgct gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag
    27421 taataacagc accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga
    27481 tcactccatt gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca
    27541 attgaactcc tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca
    27601 taattcgctc tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta
    27661 catgcaacta atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc
    27721 attgtctggg ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga
    27781 agttcttccc agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa
    27841 actaatgaca agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca
    27901 acaaaacgag caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt
    27961 attccatctt tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc
    28021 cttctaaacg cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag
    28081 aaaagacatt catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg
    28141 tacatatcat agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct
    28201 ctgtctagat ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca
    28261 aaactccttc tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat
    28321 gaagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga
    28381 aaaactggct tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag
    28441 taataagaac agccaaacaa aaatagttcc tcatattttg tcgttttttg agctaatgcc
    28501 gatggtcagc tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga
    28561 gtcacaagga gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga
    28621 tcgaggagtt ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt
    28681 gtcacagtcc cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct
    28741 gtaccaatca aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg
    28801 atcaacatag atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc
    28861 aatgcagccc cacgacgtat aaccaaataa atctactcca tctttgatag cttccttcat
    28921 ctgctcaata tgctgtttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc
    28981 ttctactttg tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct
    29041 atcatataat tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc
    29101 acttgatgga agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc
    29161 ccaattttct ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc
    29221 aacagggtag gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt
    29281 ttcttcaaac attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa
    29341 gaacaaagaa gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata
    29401 cggaacaaga agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg
    29461 acaagctttt gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc
    29521 ttgttccaaa tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt
    29581 tacacagttg atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa
    29641 tactgtttca gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata
    29701 ttctgtaacc aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc
    29761 atattttaga cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt
    29821 ttcttcatca ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa
    29881 acccatttca gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg
    29941 tcttttagga taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt
    30001 catcatatgc cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac
    30061 atcagacaca gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc
    30121 tgtcgctccg ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt
    30181 gctagttttc ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta
    30241 ctatttgtaa caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa
    30301 tctacttcca tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga
    30361 aagtatttgc catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct
    30421 tcttcactga ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt
    30481 acaggactgt agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca
    30541 ctagcaaatg ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta
    30601 cttaacacta tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa
    30661 gttagcatac aggaactgat catcgctaag aaataactag caactagcca aacaaatcct
    30721 tcaccaatat aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct
    30781 cctgcggcac cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt
    30841 cgtttatatt taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg
    30901 ataaatgcag ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca
    30961 agtactgctc ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga
    31021 ccgtagaaag aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc
    31081 actaacactt gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa
    31141 acagctaaat atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact
    31201 aataaagtaa ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata
    31261 ctttcgtgca agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa
    31321 attgctggaa taaccgtact tgtatacctt gctagaatca cgggcatccc tagaaatcgc
    31381 aaggttatgc tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata
    31441 accatagcga tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata
    31501 ggcaagaaat agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca
    31561 gtagttaata aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct
    31621 gccaagacgc ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa
    31681 atgccttttt tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt
    31741 ttggataata cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct
    31801 ccagcttcga ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc
    31861 ttattatctt ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt
    31921 tcttcgcctc cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact
    31981 tcccccttgt taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa
    32041 aacggtttct ccattcaatt agtacccctt ataatagcga acgaaactgt acaatagtgt
    32101 gtatggttta cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt
    32161 ctcttatagt caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt
    32221 ggtatctcaa gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac
    32281 aacaaagtct cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc
    32341 tgaatggaat ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca
    32401 agcagttttg ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa
    32461 atctcccaag attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca
    32521 aaaataagct cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga
    32581 tttttgaaaa gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt
    32641 tgaatctgtt tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc
    32701 tccccttgtt ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa ataagcgctt
    32761 tcagctaaat tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt
    32821 tctaaaggat aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca
    32881 tccatcctcg ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat
    32941 aacgaaacag gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc
    33001 tctatttctt tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt
    33061 tcaattgctt gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt
    33121 gtgagggtct ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct
    33181 tctcttcgaa gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa
    33241 acaatataat tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata
    33301 ttggaaaaaa ttgtactgat ttgttgaggc aaccttgcgt gttggatcac cgtttctgtt
    33361 ccaacaagag ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt
    33421 gtcgacgaaa cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt
    33481 atttgtaatg atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg
    33541 acataccctt tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg
    33601 gaatagattt tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt
    33661 gattctaaaa aataacctga acgtttattt gactggataa tgattttctt ctcgtagata
    33721 gcaagcatat ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct
    33781 aattcttcgc ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt
    33841 cgtttattta agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct
    33901 ctcaaatgac aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc
    33961 aaggttttta ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa
    34021 tctcctaaag cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt
    34081 cctcatttcc cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat
    34141 gatttggcaa ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc
    34201 acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc
    34261 attttcaact atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg
    34321 aaaccctaga ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg
    34381 attatcgaaa ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac
    34441 agcgctacga taataactaa atgcaatata atctgccgtg ttagcttgca agcactgttc
    34501 gtctccatct aacattactg gtgcacagtc ttcttccttc catgtcctgt aaatataagc
    34561 tggataatgc cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag
    34621 tgaaccaaat acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct
    34681 acacataata cccatcttag catttggatc gattttcttt aataattgag tagcacgggc
    34741 gttcgctaga aacatatgat gacaggcttg ataaatcact ttttttgtta gtgtagcaat
    34801 gggttcaagt ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg
    34861 aaaagcatta ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc
    34921 aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc
    34981 atactctcct accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat
    35041 atcatatcga tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg
    35101 cttctgttcg tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt
    35161 taaacctaac tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc
    35221 ttcatgagaa agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc
    35281 attccactga atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg
    35341 taatttgccg tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca
    35401 taaaaaattt tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt
    35461 tcttacttga agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag
    35521 agaagaaaaa aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa
    35581 gacgatcccc gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca
    35641 acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat
    35701 aatttgacac aataaccctt aaagcttcac gtggaacagt gaacgtctgc ttttcactgg
    35761 acatattaca aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat
    35821 gctcaggcgt cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac
    35881 gcaaatcgat caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt
    35941 ctgcattgat ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc
    36001 cagcatgttc acgggcgttc cattgcatag gcgtcctagc attgtctctt cctttcgcat
    36061 tgattgattc caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat
    36121 tgattgtctc aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta
    36181 actcttctcc ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca
    36241 tcttggcact ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa
    36301 gatcgtgatt attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt
    36361 tggataatac ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc
    36421 cttcttgctg atctaatcct atgtgttcga attggaagac catggataat tcatttcttt
    36481 taggattgga ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca
    36541 tcagattttt gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag
    36601 gtccattact tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac
    36661 gaaagccccc aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa
    36721 cctgtttatt tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt
    36781 gccccgtttt ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag
    36841 gctcttttcc attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat
    36901 ttttacgggc ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca
    36961 tgatgatacg gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg
    37021 taccgaattc tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg
    37081 gagactcgta tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca
    37141 acctggaaat aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc
    37201 ttggatagat ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc
    37261 tataaatgga tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat
    37321 tgtaacgctt tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac
    37381 agctgaggaa attccctcaa ctgttttttg aaaggattga tttatgcagg gaagaatgct
    37441 gatttcacaa cttctttata aacggtgttc actcagctgt cccttggtat tagctcaaaa
    37501 aacggcaaaa tatgaggaac tattttttcg tttttttctc taatactcag gccagaacac
    37561 tgatttcaca acctctttat aaacggtgtt cactcagctg tccctcggta ttagctcaaa
    37621 aaacggcaaa atataagaaa ctattttttc gtttttttct ctaatactca ggccagaacg
    37681 ctgatttcac aacctcttta tttcacattc aatatccaca tgaatcccac aaatacaaag
    37741 aacagaccat acatgattgg agatacttct tttcctcttt tagctgctaa catggtaatt
    37801 ggatagaaga taaaacctaa tgcaatccca tctgaaatac tataagtcaa aggcatgccg
    37861 atcaagatca agaatgaagg gatcgcaatc tctaagtttc cccattcgat ttgtcgcaaa
    37921 gattgtgcca tcaatactcc taccataatc aatgctggtg cagttacttg tgaagtaaca
    37981 actgatagta atggagaaaa gaacaagcct aacaagaaga aaatccctgt agtaatagct
    38041 gtgatacctg aacgtcctcc aacggcgata ccagctgatg attctacaaa tgcaccaaca
    38101 ggagaagtac caaataagga acccgcaagc atagctgttg aatcagaagc taaagctttc
    38161 cctacacgag gcatcttgtt gtctttcatg aaacctgctt ggtttgctaa tccaactaaa
    38221 gttcctgctg tatcaaagaa agtcacaagc aagaatgtca gcactacgac ccacatttga
    38281 agagaattga tgtcccctac atgttttaat gcaactaaaa atgttggttt caaacttggt
    38341 gctgcggcaa caatttcaga aggcattgga atcaaacctg tgaccagacc taaaatcgtt
    38401 gtagctgcta tcccgatgaa gatgccacct ggtacacgcc gaacaagcaa gatagcagtt
    38461 acgactaatc caaaaatcgt caaccaagta gaaccaacgt tgagtgatcc taatcctact
    38521 aatgtagatt cattcgaaac gatgatccca ccttcgctca gaccaaggaa agcgataaat
    38581 agaccgatac cgccagaaat cgcatatttc aaatctgctg ggatcgcatc aatgatcatt
    38641 tcccgcagct tgaagattgt gatcaaaata aaaatcaaag aagcaacgaa tacgccggct
    38701 aatgctgttt cccatggaat ccccattcct aaacaaacag aataagcaaa aaaagcattg
    38761 atcccaagag caggtgccgt tccaattgga taacgggcta atacccccat taaaacacag
    38821 ccgatcgcac tggctaatgc agtcgcagta aacactgctc cttcatccat tccagaagca
    38881 cctaatacag ttggatttac aaataagata taagccattg agataaatgt tgtaaatcct
    38941 gccagtacct cacgttttac cgtggtgttc aatttttcta tttcgaagta ggaaagtatt
    39001 ttttccttca tcttccatcc ccctaaatcc gaacattata aataaagtcc gttaaaattt
    39061 cgatttatta ataaaacaac gtgttttatc atatactaaa acaaaacata agtaaacaaa
    39121 aaattcggat tttaagcaaa atccgaacgt tccatctgat taaatccctt tatctcaaag
    39181 ttcatgaaca ttcactttgg attacaagaa tttctttatt gtgttaataa agtttcttca
    39241 ttctagtctc aaaaatcaat agtgtgattt gattttacca tttcgtttct gttcttcttt
    39301 gatgaattct tttgtatcga caggattcag attatttagt ccgccaaaac ctgtatcgac
    39361 catttttccc cagccttttg tcttcttttt tcctgatttg agattctttg ctcgaagctt
    39421 ctcactatgt tttgctcgcc gatacttcaa ataaccttct aatcttccca ttagatccac
    39481 tgtttttcct cctttttatt caatcagacg actcagcttt tcctataaca aaataattgc
    39541 cttcagggtc agaaaaattg aaagtcaaga tgccattgtt atctgtgatt tctcctggtt
    39601 cttccatgtt ctcgtagata gtttcaaatt catccgtaaa aaatactaaa gaaggcggtg
    39661 gtcctaatac ctctggtgaa aacttctgca caaatacttt tgggaatacg cctaattcta
    39721 attcttttga gatgctgagt acaatactct taaattcttc tggcaattcg attgtttcag
    39781 aaatttctgc accaattttt tctacaaaga atcggcaaat catctctacg tcattgacat
    39841 aaagcatcac acgtgttttt tgtatcatca tttgaccctt ctttctattt gattttcttt
    39901 cctgtctata aactgattcc attatatcac gaaagcgcaa acaccttgtg tttgatacta
    39961 ttcaagaaaa ataaagcaat agtttctagg attgctttcc tccacaaact ttttttacaa
    40021 aaaaaaaaaa cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc
    40081 tgattcatct ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt
    40141 ttccttcaac gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc
    40201 aatgaccagc accgctgctt gtgtttcttc cgtcagcatt gttctttggg cttcacgcca
    40261 gttgagacag cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc
    40321 cggagcatcg gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa
    40381 atgcagattt cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa
    40441 ggaaatacta ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt
    40501 tgcacgtttc agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt
    40561 aaatgcctga cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta
    40621 atttcttgct tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt
    40681 tgttacgtgg ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac
    40741 ttcccagaaa ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat
    40801 aattgcttga tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg
    40861 ctgtatttga taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa
    40921 acaccgagcc aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt
    40981 aaaaatgtac cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct
    41041 aaaaaatgat cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc
    41101 gcaggctctt gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct
    41161 gtaagttttt ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta
    41221 tcttttccaa atgtatctgt aaatgtttcg atactaattt gttgtagtaa gtctacatct
    41281 gaaggcgtca tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt
    41341 ttattgcctt ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa
    41401 agatattcca attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag
    41461 tagttgtttt ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt
    41521 ttcttgattt ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat
    41581 ttcttgattg ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct
    41641 tggatgattc caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa
    41701 tcgacttctt tgaattcgat attgctaata gaatctagcg ctcgggcaat cattccgatg
    41761 tctcgaagga tttcagccat ttcatcccac ctttattttg tttgtaagac tagtttacct
    41821 ctttttgttg caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag
    41881 taaagtatat tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt
    41941 tttctaaaat aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt
    42001 ctatactaaa aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac
    42061 cgtggatcgc acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt
    42121 gtttattcgt atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt
    42181 catagacttc tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag
    42241 gaagtgacca ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga
    42301 tataggcacg aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt
    42361 ttatttcttc gaaagtttct tttcctattt cactgatcag ttccaatgaa atcgtccgga
    42421 tctcctcaaa acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa
    42481 gtgcgagtag tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag
    42541 caaatgtatg actatgtaat caagtataga agactggttt aattacggca aataaatata
    42601 atgaaatctg caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt
    42661 ctctggggct gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca
    42721 aacgaaaaat atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc
    42781 tttttcacaa cctccccact aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca
    42841 atattcatca aacatgagaa gctgtttttt tgaaattgct ccttgtgact cggagctgaa
    42901 cgacaggctc acgacctctc ggtaaacagc gttcaaaagc caatttttcg atattaagcc
    42961 atttctttgt tacttattgt cattgatttc tttttctaat ttatccaata gtggagtgta
    43021 atccacttga gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc
    43081 atattttcgg tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt
    43141 cgttcgatct tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg
    43201 atctgattca cgatcacgtt tgtaccgacg ttgacataac acttcaaagt ctgctgttag
    43261 gcgaatcgtg atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg
    43321 cttatcacta aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc
    43381 aagtgctttg taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa
    43441 attgttaaac ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata
    43501 catatctgga aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact
    43561 accagccaga agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt
    43621 ccaagttcat aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg
    43681 gaatcacatt atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta
    43741 ctttcgtcca gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga
    43801 tcaattcagg ataatcttct tctttcacac ctgttcctcc atggataact agggggacat
    43861 caactactgc atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt
    43921 gtccatgatt cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt
    43981 tcactgcgtc ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt
    44041 ctgtaccgcc gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt
    44101 ctactactgc ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca
    44161 tgacagaagt atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt
    44221 ctaagtgcaa gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta
    44281 acacatgcat gccgatatat ttagcggtat cgacacttgt ttgaataatg atcggcgaac
    44341 ccatttcttt tgctgcgcct agcatagcag gaagcatttc taggttatgc gtattaaaag
    44401 ctccaactgt aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca
    44461 tcttttattg ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt
    44521 tgtcgcatct catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga
    44581 gcagcatctg cacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc
    44641 gtctgtttat tttccaaact cttttctaaa tattggatgg cttgatcgtt cacatacaat
    44701 tcagaaaatt taagtccaac ttgttcataa agtttcgcta atttatgctt atcttgggta
    44761 gtggaaatct cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc
    44821 gatagggctt tttcttcttt tgccagaact tttttcggtt ctttttcttt tttcttaaaa
    44881 aatccaaaca tcattttccc tactttcaat taaaatgctt tcaaaactgg gcttaagatc
    44941 caggcatata agagtgctgc ggctactgta tctacatcgg ttgctgtagc attgatgaat
    45001 cccatcgaat taaagatcgt tactagcaaa gcgggcaata atgtaataaa gaaaccatga
    45061 gcaataccgc caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa tatccctgct
    45121 gtccctccgg caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg
    45181 ggcaaagtaa acatggcaat aatcgtacca atcgttgtcg taatgaaacc taaaatgaca
    45241 gcatttgggc tataaggaaa gaaaactgga caatctaagg cagggatcgc atcaggaact
    45301 aatttcatag ctatccctcg aaatgccggc acgatttcac ctaacagtag acgtacacca
    45361 gctagcaata catacacacc cactacgaat tggatggctt gtaaaaaggc aaacatgata
    45421 tagttttgat ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg
    45481 atatatagcg ggaccatcac gaccatgaca gacaaataag tatcttgcag aaattcgaaa
    45541 tgtgtcggca acttgatatc ttcgatcgac tttttatttt cgcctttctc accaaaaagc
    45601 ttagcaacac cggcttcaaa taagtacccg atcgtacaaa aatgacctaa agcaatatca
    45661 tttgatccag tgattttacg aatgatcggt tgcgctactg ccggcatagc aatggcgaaa
    45721 caagctccga taaaaccacc gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag
    45781 ccaaagactg tcgtcatcgt tgccatccat aaaattgctt gaccagttaa aaaaatgtac
    45841 ttccattttg tgaaacgagc caaaataata ttgaatacaa aaatggctaa gaacgtcagt
    45901 gcaatatctc tacctaaacc caaatcattc attgcttgac cattgataga ttcaatagaa
    45961 gggatgatcc cttgcatatg aaatccttca gtaaagattt tcccgaaata agttaaactt
    46021 ccaacgatga tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg
    46081 ccagagatga cttgtcctgc tgatttcttc tgcagtagta gacccaacat agcaatcaat
    46141 gcaatggtga tcgatgcctg agtcaaaata ttttcgatga taaaattgat aacgcccatg
    46201 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
    46261 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
    46321 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
    46381 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
    46441 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
    46501 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
    46561 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
    46621 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
    46681 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
    46741 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
    46801 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
    46861 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
    46921 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
    46981 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
    47041 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
    47101 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
    47161 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
    47221 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
    47281 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
    47341 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
    47401 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
    47461 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
    47521 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
    47581 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
    47641 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
    47701 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
    47761 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
    47821 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
    47881 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
    47941 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
    48001 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
    48061 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
    48121 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
    48181 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
    48241 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
    48301 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
    48361 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
    48421 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaaactttg ttgcgaattg
    48481 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
    48541 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
    48601 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
    48661 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
    48721 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
    48781 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
    48841 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
    48901 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
    48961 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
    49021 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacact gtttattcag
    49081 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
    49141 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
    49201 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
    49261 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
    49321 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
    49381 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
    49441 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
    49501 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
    49561 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
    49621 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
    49681 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
    49741 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
    49801 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
    49861 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
    49921 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
    49981 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
    50041 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
    50101 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
    50161 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
    50221 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
    50281 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttggattgca
    50341 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
    50401 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
    50461 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
    50521 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
    50581 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
    50641 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
    50701 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
    50761 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
    50821 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
    50881 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
    50941 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
    51001 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
    51061 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
    51121 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
    51181 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
    51241 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
    51301 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
    51361 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
    51421 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
    51481 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
    51541 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
    51601 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
    51661 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
    51721 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
    51781 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
    51841 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
    51901 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
    51961 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
    52021 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
    52081 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
    52141 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
    52201 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
    52261 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
    52321 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
    52381 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
    52441 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
    52501 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
    52561 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
    52621 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
    52681 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
    52741 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
    52801 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
    52861 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
    52921 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
    52981 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
    53041 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
    53101 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
    53161 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
    53221 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
    53281 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
    53341 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
    53401 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
    53461 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
    53521 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
    53581 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
    53641 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
    53701 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
    53761 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
    53821 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
    53881 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
    53941 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
    54001 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
    54061 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
    54121 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
    54181 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
    54241 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
    54301 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
    54361 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
    54421 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
    54481 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
    54541 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
    54601 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
    54661 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
    54721 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
    54781 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
    54841 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
    54901 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
    54961 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
    55021 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
    55081 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
    55141 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
    55201 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
    55261 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
    55321 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
    55381 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
    55441 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
    55501 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
    55561 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
    55621 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
    55681 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
    55741 cgacaacttc gttgacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
    55801 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
    55861 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
    55921 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
    55981 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
    56041 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
    56101 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
    56161 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    56221 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    56281 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    56341 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    56401 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    56461 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    56521 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    56581 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    56641 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    56701 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    56761 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    56821 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    56881 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    56941 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    57001 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    57061 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    57121 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    57181 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    57241 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    57301 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    57361 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    57421 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    57481 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    57541 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    57601 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    57661 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    57721 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    57781 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    57841 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    57901 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    57961 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    58021 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    58081 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    58141 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    58201 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    58261 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    58321 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    58381 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    58441 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    58501 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    58561 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    58621 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    58681 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    58741 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    58801 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    58861 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    58921 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    58981 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    59041 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    59101 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    59161 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    59221 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    59281 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    59341 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    59401 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    59461 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    59521 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    59581 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    59641 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    59701 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    59761 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    59821 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    59881 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    59941 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    60001 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    60061 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    60121 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    60181 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    60241 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    60301 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    60361 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    60421 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    60481 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    60541 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    60601 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    60661 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    60721 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    60781 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    60841 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    60901 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    60961 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    61021 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    61081 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    61141 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    61201 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    61261 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    61321 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    61381 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    61441 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    61501 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    61561 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    61621 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    61681 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    61741 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    61801 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    61861 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    61921 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    61981 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    62041 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    62101 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    62161 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    62221 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    62281 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    62341 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    62401 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    62461 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    62521 tcctgaaaaa gccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    62581 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    62641 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    62701 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    62761 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    62821 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    62881 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    62941 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    63001 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    63061 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    63121 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    63181 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    63241 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    63301 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    63361 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    63421 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    63481 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    63541 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    63601 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    63661 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    63721 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    63781 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    63841 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    63901 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    63961 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    64021 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    64081 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    64141 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    64201 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    64261 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    64321 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    64381 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    64441 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    64501 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    64561 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    64621 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    64681 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    64741 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    64801 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    64861 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    64921 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    64981 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    65041 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    65101 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    65161 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    65221 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    65281 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    65341 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    65401 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    65461 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    65521 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaagagt
    65581 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    65641 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    65701 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    65761 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    65821 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    65881 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    65941 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    66001 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    66061 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    66121 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    66181 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    66241 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    66301 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    66361 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    66421 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    66481 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    66541 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    66601 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    66661 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    66721 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    66781 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    66841 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    66901 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    66961 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    67021 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    67081 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    67141 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    67201 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    67261 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    67321 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    67381 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    67441 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    67501 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    67561 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    67621 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    67681 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    67741 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    67801 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    67861 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    67921 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    67981 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    68041 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    68101 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    68161 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    68221 aatttttttt ataacatcaa atccacgtag atttttacaa acaaaaataa ataaagtcgg
    68281 atttttctga taattcaacg caaaaaagcg aacaattttt caataaatca gtcttctaaa
    68341 atagataacc taaaaaaagc gaacaaacaa ata
//
LOCUS       DAPCMT010000002        55657 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPCMT010000002 DAPCMT010000000
VERSION     DAPCMT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 55657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.002"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            43..1674
                     /locus_tag="RD743_000060"
     CDS             43..1674
                     /locus_tag="RD743_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDU0561923.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1736..2377)
                     /locus_tag="RD743_000061"
     CDS             complement(1736..2377)
                     /locus_tag="RD743_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDU0561924.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2563..2639
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2659..2967
                     /gene="rplU"
                     /locus_tag="RD743_000062"
     CDS             2659..2967
                     /gene="rplU"
                     /locus_tag="RD743_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDU0561925.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2994..3338
                     /locus_tag="RD743_000063"
     CDS             2994..3338
                     /locus_tag="RD743_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDU0561926.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3351..3644
                     /gene="rpmA"
                     /locus_tag="RD743_000064"
     CDS             3351..3644
                     /gene="rpmA"
                     /locus_tag="RD743_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDU0561927.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4078..6885
                     /locus_tag="RD743_000065"
     CDS             4078..6885
                     /locus_tag="RD743_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDU0561928.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7076..7567
                     /locus_tag="RD743_000066"
     CDS             7076..7567
                     /locus_tag="RD743_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0561929.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7670..8659
                     /gene="manX"
                     /locus_tag="RD743_000067"
     CDS             7670..8659
                     /gene="manX"
                     /locus_tag="RD743_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDU0561930.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8692..9495
                     /locus_tag="RD743_000068"
     CDS             8692..9495
                     /locus_tag="RD743_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDU0561931.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9516..10430
                     /locus_tag="RD743_000069"
     CDS             9516..10430
                     /locus_tag="RD743_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDU0561932.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10513..10887
                     /locus_tag="RD743_000070"
     CDS             10513..10887
                     /locus_tag="RD743_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDU0561933.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11026..11271
                     /locus_tag="RD743_000071"
     CDS             11026..11271
                     /locus_tag="RD743_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDU0561934.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11443..12507
                     /locus_tag="RD743_000072"
     CDS             11443..12507
                     /locus_tag="RD743_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDU0561935.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12753..13178
                     /locus_tag="RD743_000073"
     CDS             12753..13178
                     /locus_tag="RD743_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDU0561936.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13171..13620
                     /gene="nusB"
                     /locus_tag="RD743_000074"
     CDS             13171..13620
                     /gene="nusB"
                     /locus_tag="RD743_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDU0561937.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13717..14571
                     /locus_tag="RD743_000075"
     CDS             13717..14571
                     /locus_tag="RD743_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDU0561938.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14572..15909
                     /locus_tag="RD743_000076"
     CDS             14572..15909
                     /locus_tag="RD743_000076"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDU0561939.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15914..16138
                     /locus_tag="RD743_000077"
     CDS             15914..16138
                     /locus_tag="RD743_000077"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDU0561940.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16140..17030
                     /locus_tag="RD743_000078"
     CDS             16140..17030
                     /locus_tag="RD743_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDU0561941.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17039..17857
                     /locus_tag="RD743_000079"
     CDS             17039..17857
                     /locus_tag="RD743_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDU0561942.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17964..18413
                     /locus_tag="RD743_000080"
     CDS             17964..18413
                     /locus_tag="RD743_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDU0561943.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18431..20113
                     /gene="recN"
                     /locus_tag="RD743_000081"
     CDS             18431..20113
                     /gene="recN"
                     /locus_tag="RD743_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDU0561944.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20168..20284)
                     /locus_tag="RD743_000082"
     CDS             complement(20168..20284)
                     /locus_tag="RD743_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDU0561945.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20357..21313)
                     /locus_tag="RD743_000083"
     CDS             complement(20357..21313)
                     /locus_tag="RD743_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDU0561946.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21484..21852
                     /locus_tag="RD743_000084"
     CDS             21484..21852
                     /locus_tag="RD743_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDU0561947.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22022..22453
                     /gene="mraZ"
                     /locus_tag="RD743_000085"
     CDS             22022..22453
                     /gene="mraZ"
                     /locus_tag="RD743_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDU0561948.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22474..23433
                     /gene="rsmH"
                     /locus_tag="RD743_000086"
     CDS             22474..23433
                     /gene="rsmH"
                     /locus_tag="RD743_000086"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDU0561949.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23438..23839
                     /gene="ftsL"
                     /locus_tag="RD743_000087"
     CDS             23438..23839
                     /gene="ftsL"
                     /locus_tag="RD743_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDU0561950.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23840..26032
                     /locus_tag="RD743_000088"
     CDS             23840..26032
                     /locus_tag="RD743_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDU0561951.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26058..27020
                     /locus_tag="RD743_000089"
     CDS             26058..27020
                     /locus_tag="RD743_000089"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDU0561952.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27034..28395
                     /locus_tag="RD743_000090"
     CDS             27034..28395
                     /locus_tag="RD743_000090"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDU0561953.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28421..29509
                     /gene="murG"
                     /locus_tag="RD743_000091"
     CDS             28421..29509
                     /gene="murG"
                     /locus_tag="RD743_000091"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDU0561954.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29524..30744
                     /locus_tag="RD743_000092"
     CDS             29524..30744
                     /locus_tag="RD743_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDU0561955.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30900..32225
                     /gene="ftsA"
                     /locus_tag="RD743_000093"
     CDS             30900..32225
                     /gene="ftsA"
                     /locus_tag="RD743_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDU0561956.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32248..33489
                     /gene="ftsZ"
                     /locus_tag="RD743_000094"
     CDS             32248..33489
                     /gene="ftsZ"
                     /locus_tag="RD743_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDU0561957.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33506..34183
                     /locus_tag="RD743_000095"
     CDS             33506..34183
                     /locus_tag="RD743_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDU0561958.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34197..34796
                     /locus_tag="RD743_000096"
     CDS             34197..34796
                     /locus_tag="RD743_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDU0561959.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34811..35098
                     /locus_tag="RD743_000097"
     CDS             34811..35098
                     /locus_tag="RD743_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDU0561960.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35147..35929
                     /locus_tag="RD743_000098"
     CDS             35147..35929
                     /locus_tag="RD743_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDU0561961.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35950..36657
                     /locus_tag="RD743_000099"
     CDS             35950..36657
                     /locus_tag="RD743_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDU0561962.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36919..39717
                     /gene="ileS"
                     /locus_tag="RD743_000100"
     CDS             36919..39717
                     /gene="ileS"
                     /locus_tag="RD743_000100"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDU0561963.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39766..41292)
                     /locus_tag="RD743_000101"
     CDS             complement(39766..41292)
                     /locus_tag="RD743_000101"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDU0561964.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41307..41954)
                     /locus_tag="RD743_000102"
     CDS             complement(41307..41954)
                     /locus_tag="RD743_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDU0561965.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42138..42467)
                     /locus_tag="RD743_000103"
     CDS             complement(42138..42467)
                     /locus_tag="RD743_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDU0561966.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42644..43372
                     /gene="sfsA"
                     /locus_tag="RD743_000104"
     CDS             42644..43372
                     /gene="sfsA"
                     /locus_tag="RD743_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDU0561967.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43388..44401
                     /locus_tag="RD743_000105"
     CDS             43388..44401
                     /locus_tag="RD743_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0561968.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44401..45678
                     /locus_tag="RD743_000106"
     CDS             44401..45678
                     /locus_tag="RD743_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDU0561969.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45741..48443
                     /locus_tag="RD743_000107"
     CDS             45741..48443
                     /locus_tag="RD743_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDU0561970.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48595..48912
                     /locus_tag="RD743_000108"
     CDS             48595..48912
                     /locus_tag="RD743_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0561971.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48942..49262
                     /locus_tag="RD743_000109"
     CDS             48942..49262
                     /locus_tag="RD743_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDU0561972.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49370..50830
                     /locus_tag="RD743_000110"
     CDS             49370..50830
                     /locus_tag="RD743_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0561973.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50898..51119
                     /locus_tag="RD743_000111"
     CDS             50898..51119
                     /locus_tag="RD743_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0561974.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51150..51332
                     /locus_tag="RD743_000112"
     CDS             51150..51332
                     /locus_tag="RD743_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDU0561975.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51332..51745
                     /locus_tag="RD743_000113"
     CDS             51332..51745
                     /locus_tag="RD743_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDU0561976.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51868..53049
                     /locus_tag="RD743_000114"
     CDS             51868..53049
                     /locus_tag="RD743_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0561977.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53120..53284)
                     /locus_tag="RD743_000115"
     CDS             complement(53120..53284)
                     /locus_tag="RD743_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0561978.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53438..53511
                     /locus_tag="RD743_000116"
     tRNA            53438..53511
                     /locus_tag="RD743_000116"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53472..53474,aa:Arg,seq:cct)
     gene            complement(53580..54719)
                     /locus_tag="RD743_000117"
     CDS             complement(53580..54719)
                     /locus_tag="RD743_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0561979.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(54833..55417)
                     /locus_tag="RD743_000118"
     CDS             complement(54833..55417)
                     /locus_tag="RD743_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDU0561980.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(55544..>55657)
                     /locus_tag="RD743_000119"
     CDS             complement(55544..>55657)
                     /locus_tag="RD743_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDU0561981.1"
                     /translation="LEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT        18269 a         9455 c        11904 g        16029 t
ORIGIN      
        1 gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt
       61 tcctcactaa aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg
      121 gctgggatca cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc
      181 ggagctgatg cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt
      241 ttatcaggcg gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg
      301 tctattgcgg caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta
      361 tttttacttg cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc
      421 gtaattacgg ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat
      481 ttatttggtg ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg
      541 ataggatttc cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg
      601 tttatctatc caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg
      661 gcaacagcag ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa
      721 acattgatca gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa
      781 gtggcagttc cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt
      841 gcttcggctg gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa
      901 ggaattggaa atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc
      961 cgtacaagcg tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca
     1021 gttgtcttac tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca
     1081 gcacttgcag gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag
     1141 gagctattta ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt
     1201 acagttatct ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt
     1261 tttgttgtca aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg
     1321 gggctgccag aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg
     1381 ttttttatgt cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt
     1441 gtcattttct ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat
     1501 gaatttcaag aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca
     1561 gaagtagaaa aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca
     1621 gttgctcatg cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat
     1681 gaaaaagaac agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat
     1741 attgctgtaa aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc
     1801 gattgtagaa aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc
     1861 attcgtaggc atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat
     1921 gatttttggt aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg
     1981 tcgcaattaa ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa
     2041 atacttcgga acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt
     2101 tattgatccg atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct
     2161 cttctcgtgt taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc
     2221 taaaaggaac atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt
     2281 taggatgaag aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta
     2341 aagaagcttc ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta
     2401 ttataagaat aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa
     2461 aagcagaagt attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac
     2521 ggttgctgtt ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc
     2581 agaacaagta ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag
     2641 aaggaggtgc tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt
     2701 gaagtgggac aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt
     2761 tttgacgaag ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca
     2821 ggtgcaactg ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc
     2881 aaatacaaac ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa
     2941 gtggttatcg acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca
     3001 aaggaacatt taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg
     3061 ctgatgctgg tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca
     3121 gcactgtcaa cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag
     3181 aagaaggcgg ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata
     3241 ttgctgagat tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact
     3301 cagaatatat ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat
     3361 ctatgaatct acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg
     3421 actcagaatc aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat
     3481 caattcttta ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg
     3541 gagacgacac tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta
     3601 acaaaaaaca agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta
     3661 aagaggttgt gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag
     3721 tggaggctac gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat
     3781 caggatgttt tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg
     3841 aaataattat atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg
     3901 atttttcgtt cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa
     3961 tcaggtaaat agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt
     4021 acaatagtct atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg
     4081 tatgtcaaag aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca
     4141 caggaaattg ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag
     4201 ctagtgcgcg aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa
     4261 acactcgtta cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg
     4321 gtctctgaag aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt
     4381 ggagctaacg gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg
     4441 ccaagaggat taaattgttt gattacagga cctaccggat caggaaagac ttattttgca
     4501 cacgcgatgt atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt
     4561 gttttcaatt gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg
     4621 tacgcaaaag gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg
     4681 gatggcggta tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg
     4741 atcttttact ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat
     4801 gaagcaaatg tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa
     4861 acttttgttc ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca
     4921 aatgagaaag tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga
     4981 aagatttctt tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat
     5041 ataggacaat tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg
     5101 caatcacccg aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg
     5161 attcaattag caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag
     5221 ataactgtag caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct
     5281 tataatctat atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat
     5341 caagaagcaa tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat
     5401 aaagaccatg gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa
     5461 atcatagaag tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt
     5521 cagcaaaact ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac
     5581 ttaggagaag aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat
     5641 gaatatgctg ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt
     5701 ccagaaagtg aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca
     5761 agcggcagaa tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc
     5821 caagttgtcc aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat
     5881 atggatccga aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga
     5941 agcggggtca tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa
     6001 agagacactg gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa
     6061 actgtacgaa aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa
     6121 aattttcgag gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt
     6181 cataagaaaa aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc
     6241 aaagaattga tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct
     6301 ttgtcgattg tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata
     6361 gctactactg ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt
     6421 attgatcaaa atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa
     6481 actgaattca tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct
     6541 gataacttca tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca
     6601 caaatctctc agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg
     6661 atgcacacag caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa
     6721 aacgaattga caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt
     6781 ctgttggaag atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt
     6841 ctagtccata atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat
     6901 acacttttat agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg
     6961 tatcgtaaaa aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt
     7021 cacacagaaa tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga
     7081 tattcgattg gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc
     7141 gaagcaaaca ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt
     7201 gagaaagttt ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt
     7261 gcagaaaatg attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt
     7321 attcaccaat ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt
     7381 gaatatcggc ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt
     7441 aaatcaacaa gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat
     7501 tcgaaaagta cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa
     7561 ggtgtagcaa gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt
     7621 attaatattt ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat
     7681 tatcctagca agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt
     7741 tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt
     7801 gagagccaaa ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt
     7861 agttgattta tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa
     7921 agataaatgg gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc
     7981 acgcttttca atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga
     8041 cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga
     8101 agacgcacaa cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa
     8161 atatgtatta gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac
     8221 aaaagcagtg caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct
     8281 gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat
     8341 tagcaaaatg atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt
     8401 atttgaaaat cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt
     8461 aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat
     8521 gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt
     8581 acgtaaagta cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc
     8641 tgaattagca aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt
     8701 atttcaataa ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat
     8761 gaatttcaat ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac
     8821 ctcgaagctg gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat
     8881 atcggtgccg ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta
     8941 gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg
     9001 gctgttgcag ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat
     9061 ttgatggatg cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt
     9121 gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca
     9181 gcacaaactg tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct
     9241 atcggtgggg gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca
     9301 aaagaagtat ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca
     9361 ttgattgctc taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa
     9421 atgggtggtt catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt
     9481 ttgaatgact attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat
     9541 caaaaaaaga tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact
     9601 acgaacgtat gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat
     9661 acaaaacaaa agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac
     9721 acccatacat tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga
     9781 atggcgcgcc tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt
     9841 tagctggtgt tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag
     9901 gtgcttcatt agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga
     9961 acattattcg ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta
    10021 aaattactga tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt
    10081 tagggatgtt cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag
    10141 ttgtatcaac tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag
    10201 gtggtgaagg aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg
    10261 aaaaggtaac aacattacaa gacaacttag accaattgat tcctggttta gctgcattag
    10321 gattaacatt gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc
    10381 ttggtttatt cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa
    10441 tatagctcgg gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc
    10501 tggagggaga aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact
    10561 tcgtttacgg gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt
    10621 taccatgcgc gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta
    10681 ctagcatcag tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa
    10741 aatggtacat acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa
    10801 tatgtagatg aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt
    10861 attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct
    10921 ttattttttt actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt
    10981 aaatcccata tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg
    11041 acattgattg ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat
    11101 tcgatgggct gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg
    11161 attttcggcg aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc
    11221 gcagtgattt tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca
    11281 aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt
    11341 aaaataaaaa gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg
    11401 ctataatgga aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa
    11461 aaattgcgca aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac
    11521 aatcttcgtt acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa
    11581 aaagcatttt ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg
    11641 tttgaaatta ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa
    11701 gaagaactgc actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt
    11761 ttagaagaaa tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga
    11821 gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg
    11881 gcatatgatc acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat
    11941 caactagact tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt
    12001 gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa
    12061 ggtgacttga tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca
    12121 cgtacattcg ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta
    12181 gatgctcaat tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat
    12241 gcagttgcta gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca
    12301 ggacatggga tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag
    12361 caatttgtac caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt
    12421 ggagtacgta ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt
    12481 tcaccaaaag aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca
    12541 gtgttcacag actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt
    12601 ttaagctgta agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg
    12661 atcggcggca gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta
    12721 tctcgtgctt aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa
    12781 acgcaaacca agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg
    12841 ggattgcagc ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg
    12901 tcactgaatt attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag
    12961 atggcttgaa agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag
    13021 caatggaaat gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat
    13081 ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg
    13141 aagaactttt tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt
    13201 cgggaaatgg cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa
    13261 gatgcgattt tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca
    13321 gagtttgttc ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa
    13381 ttggataaag tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa
    13441 atggatttgg tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca
    13501 gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca
    13561 cgtaaattcg tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa
    13621 gtcctagctt ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt
    13681 agaatgaaaa cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa
    13741 gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat
    13801 ggtatccatc ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat
    13861 gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat
    13921 cctgagacga tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct
    13981 agttttcatg gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc
    14041 ctgttagcta tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga
    14101 ctccttgcgg gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt
    14161 gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc
    14221 gtcggtaaac cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat
    14281 tctaaaaccg aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata
    14341 ggacgtggac attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta
    14401 gggatgaatc gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag
    14461 cctattgcag gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg
    14521 ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa
    14581 gaatatttaa ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct
    14641 tatttagaac gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc
    14701 catcaatatt tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga
    14761 gcattccaaa aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt
    14821 atttcattat atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat
    14881 ggaataggtg ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc
    14941 ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc
    15001 agtccaagcg gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt
    15061 gtccaattgg ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc
    15121 aggaatatcc agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg
    15181 ggtggttcga ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat
    15241 caagctaata cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat
    15301 ttagtagcag atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt
    15361 ttgtcagaag agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta
    15421 cgccagatcc agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg
    15481 cagcctgaaa gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta
    15541 tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac
    15601 caattggaac aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat
    15661 ctagaaaaac gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt
    15721 gctcaagctg ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac
    15781 agctatacga cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat
    15841 ttgctgacga tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa
    15901 ggagaataaa aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat
    15961 tgtcatgcag ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg
    16021 aggaatggaa ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac
    16081 gaaaatgatg acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga
    16141 tgagattaac tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt
    16201 ttttagatga gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag
    16261 caggcgggaa aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc
    16321 ctattgatgt tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct
    16381 tgatccacga tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca
    16441 atcataaagt attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag
    16501 cattccagct aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc
    16561 aattagctgt ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg
    16621 gcgaatcgaa aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc
    16681 atttgattcg ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct
    16741 tattgctact tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc
    16801 tactcgatgt gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta
    16861 tggaaaaaaa tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga
    16921 ttgagctgtt atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg
    16981 atggcgggct gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat
    17041 ggaaaaagaa cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca
    17101 agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa
    17161 accgggagaa aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta
    17221 tgtttctcga gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga
    17281 aggaaaaaca ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca
    17341 aaatggcgca aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat
    17401 aagacaagat cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga
    17461 agactttacc ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa
    17521 actgatgctg cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat
    17581 caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc
    17641 tcagacccat caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga
    17701 tgtgctggaa ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc
    17761 tcacttgagg aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt
    17821 ggtttcaaat gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg
    17881 agaagtatgc ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga
    17941 atatgctaag gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat
    18001 tgttgagtga gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag
    18061 gaatatctgt tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg
    18121 taccggcttc agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa
    18181 atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat
    18241 ttgttatttt gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac
    18301 gttacaagaa agaattattt tcgattatca atgatgacga taatgttttg atgattacac
    18361 gaactgaaga ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg
    18421 agcgaatgtg atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg
    18481 gctttctttt cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat
    18541 tattatcgat gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca
    18601 aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc
    18661 tgaactgatg gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga
    18721 tatgtcctta actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa
    18781 tctgagaaga atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact
    18841 gctccagcct gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca
    18901 gaagaaaaag agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg
    18961 aaaagtgcag caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca
    19021 agaagaaata gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag
    19081 agaaaaactt agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact
    19141 gacgaataat gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg
    19201 gatcgcccat ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta
    19261 tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga
    19321 gagtcgttta gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata
    19381 cggcgaatcc attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc
    19441 ttctgatttt tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct
    19501 tctacttcag caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt
    19561 ggaaaaatcc attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt
    19621 acgtttctta aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt
    19681 ttacattacg accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg
    19741 cgaactgtct cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac
    19801 aagtattatt tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc
    19861 agataaaatt tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca
    19921 agttgctgca gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac
    19981 gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct
    20041 ggctggaagt gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc
    20101 aaaaaggacc tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat
    20161 tttattttta catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca
    20221 gcattgtcca aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac
    20281 gcattgtttt caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt
    20341 ccacaagagg gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac
    20401 aaataaataa tgttaataga actagccata taaaggcatc atccctttca gcaataggta
    20461 acttaatgtt cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta
    20521 aagaagttaa aattttcata atattattca gattattcga tacgattgct gaaaaagtct
    20581 gattcatttg ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt
    20641 ccaccaatac atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt
    20701 gcgctagttc tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc
    20761 ttttttggat atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg
    20821 cttccaaagt atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga
    20881 cctgccaaat gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg
    20941 tctggctaaa gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct
    21001 ttttgggcgt caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac
    21061 ttgttttagc aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat
    21121 tgaattcttc tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg
    21181 gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat
    21241 accagatcgt ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa
    21301 aatatttgat catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat
    21361 tggaatacat ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat
    21421 gaaacagttg tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg
    21481 ttcatgggca cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg
    21541 tttggttgcc aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc
    21601 cctgatttgg cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg
    21661 acaatttcta tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct
    21721 gttttccaag catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg
    21781 cgtttggttt ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt
    21841 tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct
    21901 tgatttttaa aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt
    21961 ggtagaaagt gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc
    22021 aatgttgatg ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc
    22081 aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct
    22141 tttcggctat ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt
    22201 ggctaaaaaa gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga
    22261 acttgataaa caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac
    22321 aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg
    22381 gcaagatttt tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga
    22441 tttcggacta taagaatagg aagaggctga ctaatgactg aaacatttca acactataca
    22501 gtcatgttga aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac
    22561 tgtacactcg gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg
    22621 catctgtatg cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct
    22681 tcttatgtcg aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa
    22741 cgcttgagtg agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct
    22801 cctcagctag atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg
    22861 cgcatggatc agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat
    22921 gaattagtaa agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt
    22981 gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc
    23041 attaaacaag tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt
    23101 tttcaagctg tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa
    23161 caggcaatcc ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg
    23221 gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg
    23281 ggacttcctg tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca
    23341 atccttcctt cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt
    23401 attgcagaaa aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca
    23461 agattacgaa tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc
    23521 ttcttcgaat caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc
    23581 tgctttggaa aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt
    23641 aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac
    23701 agaaatcaat gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc
    23761 tcgtactgaa cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa
    23821 tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa
    23881 aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat
    23941 tggattgttc tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc
    24001 tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc
    24061 aaaaagagga acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta
    24121 ttcaattaaa gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga
    24181 agaaaagaat tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa
    24241 agatgcactg aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg
    24301 tagctatggg aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa
    24361 agaaggtgtc gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt
    24421 cttttcttct cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg
    24481 taaactaggt ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat
    24541 ttatcaaaaa gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc
    24601 tgtcgacgga caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac
    24661 actgatggat caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa
    24721 agcaaaaacc ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat
    24781 ggaaggattg actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga
    24841 accaggatca accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa
    24901 cgagaatgaa accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca
    24961 tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt
    25021 cgggatggtc atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact
    25081 gggatttgga caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac
    25141 attaaatatt gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt
    25201 ccaaatgatg aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata
    25261 tatcagcaaa gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct
    25321 agggcaacct ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt
    25381 cgaaagcgaa aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc
    25441 ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac
    25501 agcctacctg tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta
    25561 tctaacaatg aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc
    25621 attgatgaaa cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa
    25681 aacagaaaaa gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga
    25741 tgctcaaaaa agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca
    25801 atctactgcc aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg
    25861 tgataagctg atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa
    25921 aatcctcggt attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc
    25981 tccatatgaa aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa
    26041 ataggagaga aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg
    26101 caatgacaat tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc
    26161 aggcgattcg tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg
    26221 gcggcttagt gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa
    26281 aacagctaac tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg
    26341 gctttcttga tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc
    26401 agaaattgat tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag
    26461 gttatccagg tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta
    26521 ttttcgctct cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg
    26581 atggattagt tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc
    26641 atcagcatca gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt
    26701 tcttcgtcta caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt
    26761 tgggcggact tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga
    26821 ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc
    26881 ttacaggaaa acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat
    26941 ggtcggagtg gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca
    27001 cattatggat cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat
    27061 aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat
    27121 gaattgggcg cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca
    27181 caagagctgc tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg
    27241 gatgaagaat tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta
    27301 gcaaaagccc aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta
    27361 gcggaatgcc caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg
    27421 acaggactac tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt
    27481 gggtatccag caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa
    27541 ttgtctagct tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc
    27601 aatatctatg aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa
    27661 tggaaccttc aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca
    27721 gaactacaag aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa
    27781 gtgttggaag acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata
    27841 atggaaatat ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct
    27901 atttctgtag caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt
    27961 ttccatggcg taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat
    28021 aatgattcaa aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg
    28081 tctaaggtcg tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt
    28141 ccttcattaa aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg
    28201 gatgctggaa aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca
    28261 gttcctttag cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat
    28321 gccagctggg atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg
    28381 aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg
    28441 cggaggaaca gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca
    28501 agaaccgaat gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt
    28561 ccctgataca ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc
    28621 catgcataac atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat
    28681 cctcagtgat tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt
    28741 agtgtacgct gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc
    28801 aggtgttacc aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga
    28861 tgcggcttcc ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga
    28921 agtagcagat actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa
    28981 aacagtcttg atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt
    29041 ttttttaagt gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata
    29101 ttatgaagag atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta
    29161 tatcaaaaat atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc
    29221 aacttcgatc gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata
    29281 tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa
    29341 aatgattgca gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat
    29401 gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga
    29461 tgcttcaaaa cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc
    29521 ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat
    29581 ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa
    29641 gagccaaaaa aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa
    29701 acggttcaag aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa
    29761 gagatcaagc aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc
    29821 aatttaaaaa gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta
    29881 ttgggtatac cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca
    29941 gttgcagtat caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg
    30001 tcattaggag aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt
    30061 aaaaaagcac agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc
    30121 gatttggatg taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac
    30181 ccagtgattg aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc
    30241 cctatcttgg aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat
    30301 cagttatcat cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc
    30361 aataaaaatc tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat
    30421 aatcttgcga accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt
    30481 gtgatcgaca tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa
    30541 gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa
    30601 tcaagtgaag aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga
    30661 tcatctgatt caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact
    30721 ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa
    30781 aaaccagtaa tttatggtag aattggaagc agtgaatata tctattaata agtatatttt
    30841 aaaacggacg ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca
    30901 tggcaaaaac aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg
    30961 tggctgaata tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag
    31021 ggattaaccg aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag
    31081 tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag
    31141 caaacatgct tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg
    31201 aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc
    31261 ctgaacgtca aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa
    31321 tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag
    31381 gacctaaaac gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca
    31441 acgaaatggt gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag
    31501 attttggtac gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg
    31561 ataagcaatt gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata
    31621 tctctattgt attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg
    31681 atgcataccc agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt
    31741 cagaaccagt gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac
    31801 agattttcaa caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg
    31861 gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa
    31921 tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat
    31981 ttacaaatgt aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg
    32041 caaaaattgc tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa
    32101 caacaaacac atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac
    32161 caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg
    32221 attaattcat cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac
    32281 ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg
    32341 attgaagaaa acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta
    32401 aaaaattcaa aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt
    32461 gctggttctc agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc
    32521 gaagctttag acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt
    32581 acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt
    32641 gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt
    32701 atcgctcgtt tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta
    32761 gaagttgtag ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta
    32821 cgacaaggtg tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac
    32881 tttgctgacg tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg
    32941 gctagtggag aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt
    33001 gaaacatcta ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg
    33061 acattgttcg aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta
    33121 aatatcattc taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc
    33181 atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa
    33241 gcacaaatgc aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact
    33301 tcttcagaag aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt
    33361 cctagagttg atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac
    33421 cgcgaagaaa caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt
    33481 aaaaaataaa ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga
    33541 aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt
    33601 aacaaaatct gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg
    33661 tgcagaaaac cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga
    33721 aataaagtgg catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga
    33781 aattgattac tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt
    33841 agagaaagaa ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg
    33901 aatttcacca gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa
    33961 ggttgttggg ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt
    34021 gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc
    34081 atgtagtgaa ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc
    34141 aacatttatc cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc
    34201 cgcttttaaa cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg
    34261 aataccaaga ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa
    34321 cacagaatca atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg
    34381 cgacttcacg tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg
    34441 aactacatca atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta
    34501 cacaacctaa agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg
    34561 aagctatggc gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt
    34621 tagtagaaga aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac
    34681 tagatggaga tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg
    34741 agattgacag ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg
    34801 aggaactacg atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt
    34861 ctatagcatt atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc
    34921 agggctagga cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct
    34981 gcctttgagg attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt
    35041 agccgtacaa ggtttagcac agatcgttat atggataact gtccatttag tttattgatt
    35101 gaagcaacaa aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt
    35161 ataccagcac tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga
    35221 acaggttcaa ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta
    35281 tattttagaa acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata
    35341 tgaacaggca gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga
    35401 agattttgaa acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca
    35461 tgggaaaatc cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat
    35521 catctcggac ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc
    35581 tgcacaagta acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga
    35641 aatgattatg ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact
    35701 agacagtgtg atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga
    35761 agctggtaaa gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact
    35821 attagatatt atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa
    35881 gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt
    35941 ggtttactca tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg
    36001 cggggatata atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag
    36061 gaattgactc aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt
    36121 gagtacttca atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg
    36181 gctgataaag taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa
    36241 aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat
    36301 gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg
    36361 acagaaactg aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg
    36421 cttgagtctc aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc
    36481 tccagctacg tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac
    36541 aatgaaaatg atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca
    36601 tttgatacac aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac
    36661 agaaaagcac tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact
    36721 gcatggtgaa agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg
    36781 gctgacctcg ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg
    36841 gtaccacggc atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat
    36901 aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc
    36961 cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat
    37021 cgaatctacc aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat
    37081 ggacctccat atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa
    37141 gacattatca ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg
    37201 gatacacatg gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa
    37261 atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag
    37321 caacgagcag atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca
    37381 ttagatcctt cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc
    37441 tatatttaca aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg
    37501 gaagctgaaa ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc
    37561 aaagacggca aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca
    37621 tggacattgc cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg
    37681 gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa
    37741 atcggttggg aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg
    37801 actgcaagtc atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact
    37861 ttagacgctg gtactggatt agtccatact gctccaggac atggggaaga tgactatatc
    37921 gctggtagca aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca
    37981 gaagaagcac ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat
    38041 ctattaaaag aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac
    38101 gattggcgga cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc
    38161 gataaattcc gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg
    38221 ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag
    38281 cgtgcatggg gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca
    38341 ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt
    38401 gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc
    38461 gaatttacaa aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa
    38521 gctgttttac gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca
    38581 gaccaatatc gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta
    38641 gcaccataca aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg
    38701 agtaaatctt taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat
    38761 atccttcgct tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat
    38821 atcttaagtc aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta
    38881 gcgaatacag aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct
    38941 gtagacaaat atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg
    39001 tacgacaaat ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat
    39061 ctatcgtcat tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat
    39121 tatcaacgtc gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta
    39181 ttgactccta tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag
    39241 gagtacgttc agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg
    39301 atggatacct ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa
    39361 gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa
    39421 caacaagttc gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt
    39481 tcaccagact actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta
    39541 tttgatgatg ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc
    39601 caagttcgta aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca
    39661 aaaatcgttg aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag
    39721 ggaaaagggg gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt
    39781 tccacgttca cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag
    39841 tagttcgaaa gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg
    39901 tgtttggtcc caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc
    39961 ccaatgagag aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc
    40021 ttctgggcta ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt
    40081 ttcaaatcct tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg
    40141 aatatagatc gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga
    40201 tgttttgaat acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc
    40261 agtcaaacgt ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat
    40321 agtgaatttg cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc
    40381 gcgatagccc ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa
    40441 gttttctaac acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc
    40501 tgtacggatt tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac
    40561 ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc
    40621 gcgatcttct acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt
    40681 gttccattga gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat
    40741 ttcttttcct aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat
    40801 ttgctggttc aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa
    40861 acgattgaag ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc
    40921 aaaaaattga ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc
    40981 atctaatcgg tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg
    41041 ggattgatag taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc
    41101 acggattgtt tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt
    41161 tccgattaca gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg
    41221 gtacaattta cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt
    41281 ttcattagac atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc
    41341 ttaaagctga cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct
    41401 gcgatgtcaa cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc
    41461 aacaattctt tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac
    41521 tccaatgttt gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca
    41581 tgaggacaat gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa
    41641 gtcacatgtt ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt
    41701 tcaactaaaa aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt
    41761 ccgcttgcag tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca
    41821 agtttagaaa tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt
    41881 ttattgttga ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct
    41941 cgatttggtg tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa
    42001 caaaaaaaac gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt
    42061 tacaacaaaa aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata
    42121 tatacattgg gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg
    42181 tcataaagag tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg
    42241 actaaacgat attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa
    42301 aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat
    42361 gtaccttgat actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa
    42421 ataaaacttg agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc
    42481 aaatcactcc tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg
    42541 taccgaataa aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta
    42601 caaattatga taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt
    42661 ggaaatagct ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga
    42721 cggagaaata attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc
    42781 aggcgctgaa gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct
    42841 gattgctgta aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct
    42901 ggcaattgat ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac
    42961 ggtatacaag cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt
    43021 ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg
    43081 tgcttttcct gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc
    43141 cgcacaagat ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct
    43201 ggcaacgatc catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca
    43261 aagcggagtt caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa
    43321 gcaggccatt ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg
    43381 taaaataatg atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca
    43441 agcagcttta agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa
    43501 agcacaggaa tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac
    43561 ttttttcaat attactcata tggacaccat ttatattgct tctccgaata gtcttcactt
    43621 tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt
    43681 ttcgacgcca aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt
    43741 ttttgaagca gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc
    43801 attaaaagac gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga
    43861 ccaagtatta gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt
    43921 gatggattta ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga
    43981 agtccattat ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt
    44041 acgttatgag aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc
    44101 gtcagagatt tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca
    44161 agcacaattt catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga
    44221 aaatccaatg gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc
    44281 agcttgggga ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat
    44341 ttatgattta agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta
    44401 atgaacgaat ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg
    44461 tttatctcac cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt
    44521 atagtagcgg tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta
    44581 ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta
    44641 gctattcaag tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa
    44701 tctttggtgg gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa
    44761 atcttgattg gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca
    44821 catcaagtga aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc
    44881 caaatcattg atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca
    44941 gcgacagcag atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca
    45001 attgacgtga cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta
    45061 ccgatacgaa agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt
    45121 ctgattttct ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga
    45181 ggtgtaccgg taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta
    45241 gaacaattca aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt
    45301 ttagatatta caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct
    45361 tatttgcatc gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc
    45421 gtacaagata ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt
    45481 gaagagattt ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca
    45541 gctcctgcag taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa
    45601 tataacaaag aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa
    45661 ggagcaaggc gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg
    45721 gttgccagag gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag
    45781 aaaaccaaca aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca
    45841 atgcaagccg atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc
    45901 gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact
    45961 cttctacgca agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca
    46021 tacagcaggc aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg
    46081 gagaaactgg tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt
    46141 cagagatgct ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa
    46201 atccgcaact gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag
    46261 ctcaagatag tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga
    46321 tccaccgttt accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt
    46381 accgtccgct tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga
    46441 cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca
    46501 ccttgccgaa tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta
    46561 tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg
    46621 ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag
    46681 tctgtgcaaa atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa
    46741 aagaagaact ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac
    46801 gtctcgaccg attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg
    46861 atgttgtctg gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa
    46921 aagtcacttc tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa
    46981 aagatgtgga tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca
    47041 aagatctttt gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa
    47101 aacggttaga ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa
    47161 gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac
    47221 ggaaaaattt taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag
    47281 aagaacaagt tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca
    47341 atctaggaga aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg
    47401 gcagacagac tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag
    47461 ggaaagcttt taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac
    47521 aatatgtcaa aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg
    47581 gatcattaaa caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta
    47641 tcacgatgac aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa
    47701 gcttagaaga tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt
    47761 tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta
    47821 ctggtgaagg agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata
    47881 aggtagaact gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg
    47941 ccttattgga agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa
    48001 atatcccatt tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc
    48061 gtatagctga tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa
    48121 gcgatgtctt ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc
    48181 agaccgattt gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag
    48241 ccttcatgtt agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta
    48301 ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag
    48361 aacgaaaata tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc
    48421 ttgaaaataa aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt
    48481 gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat
    48541 tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca
    48601 aaaaaaacaa tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa
    48661 atgcaaaaag cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca
    48721 gatgcagata ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta
    48781 cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc
    48841 atcaatatgc aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat
    48901 ctaatgaaat aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc
    48961 tagaagccat catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag
    49021 cgattgcggc tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag
    49081 aagaatcact tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag
    49141 gaaatcatat gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta
    49201 ttgcgtttac tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat
    49261 aaatacaact tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt
    49321 tataaggaga actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact
    49381 atcaagttgg atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca
    49441 tttagttgct ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt
    49501 cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc
    49561 tgacagttta gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa
    49621 tggttttgtc tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg
    49681 gtataatctt gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc
    49741 taccttgatt caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt
    49801 tccttcaaat gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg
    49861 cacactgtca gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta
    49921 ttttacagtt atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc
    49981 caacattacg atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc
    50041 cattttacca gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg
    50101 tgtagctgat gggcaactga tcattgattt ggtgcaaaaa tatattgcag agccattcct
    50161 aggattatct caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg
    50221 gttctttggt attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca
    50281 agcacaacta atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc
    50341 agccgtgtta gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga
    50401 tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat
    50461 tttctctaaa cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt
    50521 caatatcaac gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat
    50581 cccattcttg attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg
    50641 cctagtcgct ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt
    50701 cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac
    50761 tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga
    50821 acaacaataa gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt
    50881 ttcgaggagg aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag
    50941 aattgttaga aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa
    51001 tcaagctgtt ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg
    51061 aactaatcaa agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga
    51121 aaatggttta aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta
    51181 ttggttcttt gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata
    51241 gtatggtcac aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa
    51301 aaagagaaga agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc
    51361 agcatcgttt ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac
    51421 cggcaaatca gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca
    51481 aaataaccgg gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg
    51541 tacatcgaca acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc
    51601 gacctgtgag gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa
    51661 tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct
    51721 agatatgatc gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa
    51781 atgaagtaag ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact
    51841 agcttttttg taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct
    51901 tcgattgagg aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa
    51961 aggcgagaac gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag
    52021 gaggatcggc taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa
    52081 gagtacctaa tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc
    52141 gggatcaatc agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta
    52201 tcttatctgg cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt
    52261 gataaaatca gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga
    52321 aaaataaagc gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag
    52381 atgccggaaa accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc
    52441 aagcaaaaag gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga
    52501 tatatcgttt atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat
    52561 catgatgctt tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca
    52621 tttgaatatt catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt
    52681 gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg
    52741 gaaaattatc catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat
    52801 tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc
    52861 tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag
    52921 ctattattag ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca
    52981 aaagaatatg aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac
    53041 tatttttgat aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt
    53101 tggcatttga aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt
    53161 tttcttttgt tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt
    53221 cccgattttt tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat
    53281 tcattggaaa ggacctccct acattatttc attctacctc tagtttatca tctgttaagg
    53341 actagtggaa acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag
    53401 taaaaaacaa acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag
    53461 agcaatcgcc tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtacaaataa
    53521 cccctgtatc ctttgtgata caggggtttg ttttgtgttt gtgccaaaaa tgtgccaaac
    53581 tagttatcat ttttatataa actatcgaac acagcattga ttttttcttt atcctgatct
    53641 tcaagctctc gaacaatatg aagataagta gacattgtag tttctaaact gctatgtcct
    53701 aaacgtttcg atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgtgta
    53761 tgccttagtc catgcaaagt aaattgcttt tcaatcccta attcttttaa ttttttgcgc
    53821 aaatatttgc tgacggcgtt tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa
    53881 ttatgagggt ttttaacttc aaaattctcg aataattctt tttgattgat tttaaacttt
    53941 tttaagagat caatcgtatg tttatcaatg gaaattttcc tatttgaagt ttcgtttttt
    54001 gtttttcccc attcatttaa tttataattc catgttttat taatagtgat gatttgatct
    54061 tcgaaatcga tatcttccca tgttaatcct agaagttcag caaatcgcat tccagtagca
    54121 ccagcaacta acacgagcat aggagaagag tactgcgcag aaaggttttc ttctacgagt
    54181 ttcattaaag atttaaattg atcataatct aaatatttat cttcttttgg cttcaatgaa
    54241 tcttttcctt tgattactgc ttttctagtt ggatcaaacg gaattaatcc ttcttctacg
    54301 gcatctttca acgatgctct aatatggtta ttaaacttaa taacggtgga ttttacatgg
    54361 tctttcgcat acttatttaa aaagcgttga tatccaactc tatctaaatc agaaatcaat
    54421 accgctggca tatatttctt tatgttcatt aacgtatctt cgtattttct ataagtgata
    54481 ggagatactg ttccttcttt ataaagttgc atccaatcct caaagtaatc tgataataat
    54541 agatttttgc gatccccttt aaggcctttg ttcaagttat attctaattc gttggcggca
    54601 tcttttgctt cgccttttgt cttaaatcct gattttctta tcttcgtgta ttttccgtca
    54661 tcttttttgt atgaaatttc gtattgccaa ctatttccac gtttgactaa tcttgccata
    54721 attgaatcta ctctctttct ctgatacaat aggcactata aagaagccta ctgtataggt
    54781 ttgtttttct tagaacacgc ttgctttgga cggtgggcgt gttttttatt tattatacag
    54841 ttgctttaaa aatagctgct ttttcaaatg atactaaagg agagaagtgg atttctattt
    54901 ctcccttttg gtttaacgtg aaatgagcag ttacatccat ttgttttcct ggagcaacag
    54961 atcccattgt attttcgttg gcatatgtct ctgatttttt gtcatctggt ccatatactt
    55021 ccacatctgt acctacaggg atatctgaat cgccatcatt ttttacggta taagtaattt
    55081 ttactacttg tgcaggttgg ttttcttcaa attgatttct ttcatcagtt agttctacac
    55141 tatttagtgt atattcagca tcaccaactt ttacagtgtc accaatctta tagaaggtat
    55201 cgcttttttc ttctttagaa gatgaagcag aggttgaaga tttagttact ttttcgccac
    55261 cattatcact tgctttatta ctatctgatc caccatttaa agcagaacca ataattataa
    55321 ttaaaattac tgctaatacc caaaaccata cttttttgta aaaaggttta ctaactttat
    55381 acatttttcc gtcttgaccc ataacttttt ttgccattta aatattcctc acttcttgtt
    55441 ataatatatt tgcgatctca gaaatgaggt atgagtccgt gtgccagcac gggctttttt
    55501 aatgttttgc tgaaatcggt ttttttaata attcttgatg tattcataca tattgccttg
    55561 cgtaagaata ttttttctta aaatagcatt ggcagacagc ataacaagtg tatctgagct
    55621 tattctgaat agaataatat tccataaatt tttcgag
//
LOCUS       DAPCMT010000003        52525 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPCMT010000003 DAPCMT010000000
VERSION     DAPCMT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 52525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.003"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            44..214
                     /locus_tag="RD743_000120"
     CDS             44..214
                     /locus_tag="RD743_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0561982.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            434..1141
                     /locus_tag="RD743_000121"
     CDS             434..1141
                     /locus_tag="RD743_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0561983.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1201..1845)
                     /locus_tag="RD743_000122"
     CDS             complement(1201..1845)
                     /locus_tag="RD743_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDU0561984.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            1972..2259
                     /locus_tag="RD743_000123"
     CDS             1972..2259
                     /locus_tag="RD743_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0561985.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2367..2687
                     /locus_tag="RD743_000124"
     CDS             2367..2687
                     /locus_tag="RD743_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0561986.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2768..4081
                     /locus_tag="RD743_000125"
     CDS             2768..4081
                     /locus_tag="RD743_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDU0561987.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4078..4797
                     /locus_tag="RD743_000126"
     CDS             4078..4797
                     /locus_tag="RD743_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDU0561988.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4925..5476
                     /gene="raiA"
                     /locus_tag="RD743_000127"
     CDS             4925..5476
                     /gene="raiA"
                     /locus_tag="RD743_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDU0561989.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5698..8232
                     /gene="secA"
                     /locus_tag="RD743_000128"
     CDS             5698..8232
                     /gene="secA"
                     /locus_tag="RD743_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDU0561990.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8358..9459
                     /gene="prfB"
                     /locus_tag="RD743_000129"
     CDS             join(8358..8429,8431..9459)
                     /gene="prfB"
                     /locus_tag="RD743_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDU0561991.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9564..10250
                     /gene="ftsE"
                     /locus_tag="RD743_000130"
     CDS             9564..10250
                     /gene="ftsE"
                     /locus_tag="RD743_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDU0561992.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10234..11127
                     /locus_tag="RD743_000131"
     CDS             10234..11127
                     /locus_tag="RD743_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0561993.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11279..12727
                     /gene="cls"
                     /locus_tag="RD743_000132"
     CDS             11279..12727
                     /gene="cls"
                     /locus_tag="RD743_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDU0561994.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13064..13921
                     /locus_tag="RD743_000133"
     CDS             13064..13921
                     /locus_tag="RD743_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDU0561995.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14036..14956
                     /gene="pstC"
                     /locus_tag="RD743_000134"
     CDS             14036..14956
                     /gene="pstC"
                     /locus_tag="RD743_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDU0561996.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            14956..15840
                     /gene="pstA"
                     /locus_tag="RD743_000135"
     CDS             14956..15840
                     /gene="pstA"
                     /locus_tag="RD743_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDU0561997.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15855..16661
                     /gene="pstB"
                     /locus_tag="RD743_000136"
     CDS             15855..16661
                     /gene="pstB"
                     /locus_tag="RD743_000136"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDU0561998.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16677..17435
                     /locus_tag="RD743_000137"
     CDS             16677..17435
                     /locus_tag="RD743_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDU0561999.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17448..18125
                     /gene="phoU"
                     /locus_tag="RD743_000138"
     CDS             17448..18125
                     /gene="phoU"
                     /locus_tag="RD743_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDU0562000.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18363..19394
                     /locus_tag="RD743_000139"
     CDS             18363..19394
                     /locus_tag="RD743_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDU0562001.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19391..20158
                     /locus_tag="RD743_000140"
     CDS             19391..20158
                     /locus_tag="RD743_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDU0562002.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20258..21826
                     /gene="liaX"
                     /locus_tag="RD743_000141"
     CDS             20258..21826
                     /gene="liaX"
                     /locus_tag="RD743_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDU0562003.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22123..22443
                     /locus_tag="RD743_000142"
     CDS             22123..22443
                     /locus_tag="RD743_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDU0562004.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            22443..22793
                     /locus_tag="RD743_000143"
     CDS             22443..22793
                     /locus_tag="RD743_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDU0562005.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            22960..23898
                     /locus_tag="RD743_000144"
     CDS             22960..23898
                     /locus_tag="RD743_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDU0562006.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23913..24743
                     /locus_tag="RD743_000145"
     CDS             23913..24743
                     /locus_tag="RD743_000145"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDU0562007.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24787..25812
                     /locus_tag="RD743_000146"
     CDS             24787..25812
                     /locus_tag="RD743_000146"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDU0562008.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25827..26765
                     /gene="galU"
                     /locus_tag="RD743_000147"
     CDS             25827..26765
                     /gene="galU"
                     /locus_tag="RD743_000147"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDU0562009.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26890..27330
                     /locus_tag="RD743_000148"
     CDS             26890..27330
                     /locus_tag="RD743_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDU0562010.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27333..27935
                     /locus_tag="RD743_000149"
     CDS             27333..27935
                     /locus_tag="RD743_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDU0562011.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(27994..29100)
                     /locus_tag="RD743_000150"
     CDS             complement(27994..29100)
                     /locus_tag="RD743_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDU0562012.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            29310..30311
                     /gene="ccpA"
                     /locus_tag="RD743_000151"
     CDS             29310..30311
                     /gene="ccpA"
                     /locus_tag="RD743_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDU0562013.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(30648..32993)
                     /locus_tag="RD743_000152"
     CDS             complement(30648..32993)
                     /locus_tag="RD743_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDU0562014.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            33272..34174
                     /locus_tag="RD743_000153"
     CDS             33272..34174
                     /locus_tag="RD743_000153"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDU0562015.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            34387..36162
                     /locus_tag="RD743_000154"
     CDS             34387..36162
                     /locus_tag="RD743_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDU0562016.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(36228..37814)
                     /locus_tag="RD743_000155"
     CDS             complement(36228..37814)
                     /locus_tag="RD743_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDU0562017.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            38065..38289
                     /locus_tag="RD743_000156"
     CDS             38065..38289
                     /locus_tag="RD743_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDU0562018.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            38431..40182
                     /locus_tag="RD743_000157"
     CDS             38431..40182
                     /locus_tag="RD743_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0562019.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            40182..41966
                     /locus_tag="RD743_000158"
     CDS             40182..41966
                     /locus_tag="RD743_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562020.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            42292..43143
                     /locus_tag="RD743_000159"
     CDS             42292..43143
                     /locus_tag="RD743_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0562021.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            43197..44948
                     /locus_tag="RD743_000160"
     CDS             43197..44948
                     /locus_tag="RD743_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0562022.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            44952..45122
                     /locus_tag="RD743_000161"
     CDS             44952..45122
                     /locus_tag="RD743_000161"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0562023.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(45162..46064)
                     /locus_tag="RD743_000162"
     CDS             complement(45162..46064)
                     /locus_tag="RD743_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDU0562024.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            46295..47698
                     /locus_tag="RD743_000163"
     CDS             46295..47698
                     /locus_tag="RD743_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDU0562025.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            47698..49131
                     /locus_tag="RD743_000164"
     CDS             47698..49131
                     /locus_tag="RD743_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDU0562026.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            49276..50202
                     /gene="trxB"
                     /locus_tag="RD743_000165"
     CDS             49276..50202
                     /gene="trxB"
                     /locus_tag="RD743_000165"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDU0562027.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            50416..52143
                     /locus_tag="RD743_000166"
     CDS             50416..52143
                     /locus_tag="RD743_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDU0562028.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            52158..52508
                     /locus_tag="RD743_000167"
     CDS             52158..52508
                     /locus_tag="RD743_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDU0562029.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT        17027 a         9217 c        11275 g        15006 t
ORIGIN      
        1 ttaagataga gttaagttaa ctacaagaat tggagggatc cacatgaaat ggaaagaaaa
       61 agtagacgaa gcagctgaaa agttatatga tttggtcaaa tcagaaaaat acaatattga
      121 agtgaatatt ccgaaaaaag gcggaaaagc ggttcaggta aaatcaaaac gcccaacgaa
      181 tcatacaaaa aaatggatgg ctaaaaaccg ctaataataa caaaagatcc ataagtcggc
      241 attttcatcg attttagggt cttttgttat tttctaggtt gaacatatct aaaaaattgt
      301 ttctgacaat aggagtagga agaacgatgt gtgtctaaat cctctgttga tggatagaaa
      361 cctttatctt tttcagttgt gaatcaggta tataccagtt ataatagaag aaacgaaagg
      421 acgggtgtac gttatgtcga atcaattacc tgtatatata cagattcatg accaattaaa
      481 aaatgaaatc gaacaaggta tatggaaagt cggagaccgt cttccttcag aaagagaatt
      541 agcagtgaaa ttcaatgtca gccgtatgac gttgagacaa gcaattcaaa acttggcaga
      601 tgaaggaatc ttagaaagaa agatcggttc gggtacatat gttgcgcgag aaaaagtaca
      661 agaaaaaatg tctggtgcaa caagttttac agaaattatg gaatcgcaaa atcgagtacc
      721 gtctagtcgg acaatctctt atttcttgac ttcgccaagc tcaagtgaaa tggaaaaact
      781 aaacttgagt aaagatgaca cgatcttgcg aatggaacgt attcgtttcg cagatgatgt
      841 ccctatttgt tttgaagttg ccagtgtgcc acaaaagctg atcgatggct acagcaaagc
      901 agagattact gaatcgttgt atcgcacgct agaagaaaaa ggcgggaaaa agatcggtcc
      961 agcgaatcaa acgattactg ctgtgttggc ttctgagaaa attgctgact atttagaggt
     1021 aaaacgagga gatgcagttc ttcgtttgcg ccagatttct tattttgaag acggtacgcc
     1081 gtttgagtat gtgcgtacgc aatacgtagg aaatcgtttt gagttttatt tggaaaaata
     1141 aaaaacaggt gatccctgat tccttactta gaaataaaag agaatcagaa tcacctgttt
     1201 ttatatatga tcagttacca gttgctcgtg ataagaaaca ggaccctctt caaattggac
     1261 ttgtccgttc agcagctcaa tgatggattc tttaaagtgg tcagcacttt tttcatctac
     1321 agcacagatg acgctaactt gatcagtata ttggatttct tttataaaaa aatgttggag
     1381 ttcaagataa ttctgtaatt ttcctagttg cggatacgtg atcatcacag agatctcctg
     1441 ttgcaaggta ccttctacga ttccaatgtc agacaatgct tgagagacag catgagtgta
     1501 tgctcgtatt aatccgcctg tgccgagttt cgttcctcca aaatatcgag tgactactgc
     1561 agctacattg atcagttgat tttttttgag gacttccaac atcggtacgc cagccgttcc
     1621 acttggttct ccatcatcgc tgcttcgctg gatttcattt ttttctccaa tgatgaaggc
     1681 gctgcagtta tggttcgctt tccaatgttc tttttttatt tcttgaatga aactttttgc
     1741 ttggtcctct gattctattc gtctaagaga acagataaag cgtgattttt tgatctcaat
     1801 ctcactttgt ccattttctt ttattgtacg atagcgctcg atcatcttgt cacctctttt
     1861 tttcattttc tttattcagt ataaataaag ttctaggttg tttcaacact gtaacggttg
     1921 cgcaatcgct gtagtatgcg ataataaaga caaaggtagg tggatctggg gatgcaggaa
     1981 cgaatgaaaa agtacgatgc aattacgcat tatttgaaga acaatggcgg ctctcaagta
     2041 acgttgacct ttacccaatt tgacgaactg ctgtttccca gtaacggact tcccaaaaca
     2101 gcaagggaaa gtacagattg gtgggcaaat gattataaac accctgaaaa aggtgcctat
     2161 ggctggataa atgcaggcta tgaagtagtt gtcatcaatt tggacaaaga atatgtcgtg
     2221 ttcaataaat tagtgaaatc tagttggcta tttgattaaa taaaccgcgt ttagaaaacg
     2281 ttgacaaatt tctttctttt tatataatag agtaacagac gcgaagaaaa cgtttgcgta
     2341 gtttgtttta aaaaggagag aaaagtatgg ctaaaaaaac aatcatgctt gtatgttcgg
     2401 ctggtatgag cacaagttta ctagtaacaa aaatgcaaaa agctgctgaa gcaaaaggaa
     2461 tcgactcgga tattttcgct gtgtccgctt ctgacgcaga taacaatctt gcaaataaag
     2521 atgtagatgt actgttatta ggacctcaag ttcgctttat gaaagctgat tttgaaaaac
     2581 gtctggaacc aaaaggcatt cctttagacg tcatcaatat ggctgattat ggtatgatga
     2641 atggcgaaaa agtgttagat caagcaatca cgctcatagg tgaataaata gaacagtata
     2701 actatcgaac aagcgatcca aaaaatagat cgcttgtttt ttcgtatatt tttttagggc
     2761 gaataatatg aatcattttt ggggtagaag gcttctgacg agagatatta aagcaataga
     2821 ctgtgaggag gtaaatttac caaattatga gaagatcgca gcagtggaac gtttgggcaa
     2881 ctggattctt tgtcatcgct gtggaatgag gacgtccgtc attgatggac agcttgcaga
     2941 ttacggttat ttttgtacac attgtctata tttagggcga tgcgacactc aacaagattt
     3001 atatttgttt gatcagcctg aagcaaaacc tcgagaagtc gtcttttttt ggacaggaca
     3061 gcttactgaa aaacaaacgg aaatcgcaaa gagaatactt tgccattcag aaaaaagaca
     3121 tcatcttatt tgggctgtaa caggtgcagg gaaaacagag atgctttatc ctattttagt
     3181 ggaaacttta aaagctggag gaagagtagc gatttgtaca ccgcgagtcg atgtttgtaa
     3241 tgaattgttt ttacgctatc gttatgtttt tccagaagaa aaaatcacgt tgctccatgg
     3301 gaatacagca acggcttata cctttacttc ttttgttatc tgtacgattc atcagctata
     3361 ccgtttttat cggtcctttg atttaatagt gatcgatgag atcgatgcgt ttccttataa
     3421 cggggatgca ggattagctc atgctgtgca aacagcgatt aaaccagacg gacgattcat
     3481 ctacttaagt gccacacctg ataaaaagtt actaaaggaa atcaagcaga cgtttgacat
     3541 ccacaaactt gctttgcgct ttcatcggcg tttacttcct gaacccgtct tggttttcat
     3601 gaataattgg cgacaaaaat gcaagaacaa gaagaagata aaatctttgg tgcggatcat
     3661 tcatcaatta attaaagaaa accatatcct tttattttgt ccaagtatca cgatgatgca
     3721 tcgtttgaaa gccacacttc aagaggctct tccgagatat gagatcacag atgtttcttc
     3781 aagagacaat cagcgggcgg aaaaagtcca gaaaatgcga gaagcaaaat atgacatact
     3841 gttaaccacg atgatccttg aacgtggagt cacatttgaa agaatatccg ttatcgtttt
     3901 aggagcggat catccggttt ttactaaatc ttctttggta caaatcgcag gaagagccga
     3961 tcgtaaaggg ccttatacga acagtcaagt atactttttc tatgaagaaa aaactgcagc
     4021 tattcgaaaa gcttgtcagg aaatcaaaga aatgaatgaa gaagggaaga agatgctgtg
     4081 aggtgcagtt gctgcagtaa agatatcgtc caaaatttaa cattcagcga gattattggt
     4141 gtttcgaaat acagattacc tcagacttgt tcagtttgtc gcgcacaatt tcatttattg
     4201 gaaacaagag gctgttctgg ctgtcaaaaa ccaatccatt tcaatctaga gagtacgaaa
     4261 aagaaagtaa agtctattcc cgtttactgc agtgactgtc aagaatggaa gaagaaatat
     4321 ccgacctatg attttcagca ccgtgccttt tttgtgtacg atgaaaatat gaaggcctgg
     4381 ctcaatcaat acaagtttat gggagatatc cgactagcag gcacctttgc atataaatgg
     4441 acgcagataa aaaaagaata cccgacatac atctattgtc caataccttt atctgaaaaa
     4501 aggcgaacag aaagaggatt caatcaagta agcgagatgt tgaaagcagc aagaattcct
     4561 tttcagatgc tactcaaaag agagaagcat ctgctggctc aagcaaaaaa cacgcgaaag
     4621 gaaagattgg agatgcctca accttttgaa cttgcagtca ccttttctaa gatacaaaat
     4681 aaaaaaatat tgattattga tgatgtctat acaacaggac agactctttt ccacgcagca
     4741 gattgtttgc aggtcgcaca gccgaaaatc atccgaacca tttctttagc aagatgaatt
     4801 agatgtggaa atttgatagc gatttaattt gcaaataacc tagagttcga tataatagaa
     4861 ttaagaagag ggaaagagtg gtccttctcc ttcttgattg tggtagacga aaggggtaat
     4921 ttttatgttt agatataatg tacgcggcga aaatatcgaa gtaactgaag cgattcgcga
     4981 ctacgttgag aaaaaagtag gcaaactaga acgctatttc agtgacgcac ccgatgcaac
     5041 ggcgcatgtg aacttaaaag tttatacaga aaaaacagca aaagtggaag taacgatccc
     5101 attaccttat cttgtattac gtgccgaaga aacttctcca gatttatacg caagtatcga
     5161 tttggttgtt gacaaattgg aacgccaaat ccgcaaattc aaaacaaaaa ttaatcgtaa
     5221 atcccgtgaa acgggcatga atactgcaga tgctactgct ttattcaatg aagaggacga
     5281 aaacgaaaat gattctgaat tagatatcgt acgtacgaaa cgcctttcat tgaaaccaat
     5341 ggatagtgaa gaagcagtct tacaaatgaa catgctggga cataacttct ttatttttga
     5401 agatgcagaa acaaatggta caagtatcgt ttaccgccgt aaagatggaa aatacggact
     5461 gattgaaaca gactgatcag ccttacttaa ataacaacga aaacttgcag cagctactga
     5521 cagctgccgc aagttttttt gagtttcctc ctcttttaga tgaaatatga ataaattatg
     5581 aagttcttac tttaatacgc ttaagatatt tcaattatct ataactaatg ataaaatgaa
     5641 aagtaaagtc tacatagtct ggataataca cagaaggaaa aggagattac acaacgcatg
     5701 gccaatttga ttcgttcact tatcgaaaac gataagaaag aactaaaacg tttagatagc
     5761 attgcaaaaa aagttgaagc atacgctgac caaatggcag cattaactga tgaacaatta
     5821 caggcaaaaa ctgctgaatt caagggcaga taccaaaaag gagaaacatt agatcaatta
     5881 cttccggaag cttttgcagt cgtccgagaa gcagcaaaac gtgtattggg gttatatcca
     5941 caccacgtac aattgatggg tggaatcgtg cttcacgatg gaaatatccc tgaaatgcgt
     6001 actggtgaag gtaaaacctt gaccgcaacg atgccagtat atctaaatgc tctgactggc
     6061 gaaggggtac acgtagttac cgtcaatgaa tatttagcaa cccgtgactc gacggaaatg
     6121 ggcgaacttt ataatttctt aggattaaca gtggggttga atatcaactc aaaatcttcg
     6181 gatgaaaaac gcgaggctta taattgtgat atcacttata gtacgaataa tgaattagga
     6241 tttgactact tgagagacaa tatggtcgtt taccgccatc aaatggtaca acgtccgctg
     6301 aattatgcca ttgtcgatga agttgactca atcttgattg atgaagcgag aacgccgctg
     6361 atcatttcag ggcaagcaga aaaatccaca gctttatata caagagcaga caacttcgtg
     6421 aaacgtttga aagaagaaga agattataaa atcgatgtac aatcaaaaac aatcagttta
     6481 accgaagcag ggatcgaaaa agcagaagaa acattcggtt tggataactt atacgacatt
     6541 gaaaatactg cattgaccca tcacttggat caagcattga gagccaacta tatcatgctt
     6601 ttagatatcg attatgttgt ccaagacgga aaagtactga ttgttgacca gttcactggt
     6661 cggatcatgg atggacgccg ttattcagat ggacttcacc aagcaatcga agctaaagaa
     6721 ggcgtagaga tcgaagacga aacgaaaacc atggcgacga tcactttcca gaactatttc
     6781 cgtatgtaca agaaacttgc gggaatgact ggtacagcta aaacagaaga agaagaattc
     6841 agagaaattt ataacatcca agtataccag attccgacaa atcgtccggt tatccgtgat
     6901 gatcgtccag atttgcttta cccaacatta caaagcaagt tccacgctgt cgtgcaagat
     6961 atcaaagatc gctaccgcaa aggtcaacct gtgttagtcg gaactgttgc ggttgaaaca
     7021 tctgagttat tatcagatat gctgaatcaa gaacgtattc ctcatgaagt gctgaatgcg
     7081 aaaaatcact ttaaagaagc agagatcatc atgaatgctg gtcaaaaagg tgctgtgacg
     7141 attgctacca acatggctgg tcggggaaca gatatcaagt taggacttgg ggtaagagaa
     7201 cttggtggtc tagcagttat cgggacagaa cgacatgaat ctcgtcgtat cgataaccag
     7261 cttcgaggac gtgcaggacg tcaaggagac cctggtatgt cacagttcta cttgtcattg
     7321 gaagatgatt tgatgaaacg attcggttca gaacgaatca aatctttctt agatcggatg
     7381 aagatcggtg aggaagatgc tgttatccaa agcaagatgc tgacgaaaca agtagaatct
     7441 gcacaaaaac gtgtagaagg aaataactat gacacacgta aaaacgtctt acaatacgat
     7501 gatgtgatgc gtgaacaacg tgaagtaatc tataaacagc gacaagaagt aatcatggaa
     7561 gaaaaaagct tatccaagcc actaatgaac atggtcaaac ggacaatcag tcgtgtagta
     7621 gatgcgcata cacaattaga gaaagaaaat tggaatttgg aaagtatcat cgattttgcc
     7681 ggcagtactc ttgtccatga agatagtatt actcttgcag atatcgaagg aaaaacagct
     7741 caggagatca aagagtatct aaacgaacga gcacaagaag tatttaatac caaagcggct
     7801 caactaaatg gtccagagca actgttggaa tttgaaaaag tcgtgatctt gcgtgttgtg
     7861 gactctaaat ggacagacca tattgatgca atggatcaat tacgtcagtc aatcggctta
     7921 cgtgcttatg gacagaacaa tccattagtc gaatatcaaa cagaaggcta taagatgttt
     7981 gaagatatgg taggcgccat cgagtatgaa gtcactcgaa tctttatgaa agctgagatc
     8041 cgtcaaaacg tacagcgtga acaggtttct caaggaacag cttctcactc agaagacgga
     8101 gatgccaaag aaaatagtaa tactagcgcg aaaaaacagc caatccatgt gaacaaggtc
     8161 ggaagaaatg atccttgtcc ttgtggaagc gggaaaaaat ataagaactg tcatggaaaa
     8221 aatgcaaact aatttctgac agcaggattg gtgagacaaa ttcttttttt aagaattcgt
     8281 ctcaccttct ttctgttatt tgctgattct gttagactag atataatgaa aaaagtatag
     8341 tgaaagaagg aaaaaacatg gaaatatccg aaattcgaaa tctcctgaca gaaatgcagg
     8401 aaaaggtcac aagcttcagg aggtctcttt gacttagacc agctggaaga agacattgcg
     8461 gaagcagaag caaaaatggc tgaacctggt ttttgggatg atagcgaagc tgctcaaaaa
     8521 gtgatcagtg aaaacaatgc aaataaagaa acttatgatc aattccattc tcttgctgaa
     8581 gaactggaag aaatcgaatt gatggtggag atgttgcagg aagaacctga tgcagccatg
     8641 caagaagaag cagaaacacg tatcaatgcg ctagatgaaa aaatgaagac atatgaatta
     8701 tctatgttgt tagatggtcc ttatgatcga aacaacgcca tcgttgaatt gcatccaggg
     8761 gctggcggaa cggagtccca agattggggc agtatgcttt tacgtatgta tacacgctgg
     8821 gcagagcagc atgggtatca agtagagaca ctagattatc aagcaggtga tgaagcagga
     8881 atcaagagtg tgaccctact gatcaaagga cacaacgcat ttggttatct gaaatcagaa
     8941 aaaggtgttc atcgccttgt acgtatctcg ccttttgact cagcgaaacg acgtcatact
     9001 tctttttgtt cggtagatgt gatgccagag cttgatgaaa cgatcgatat agaaatcaat
     9061 ccagatgatt tgaaaatcga tacgtaccga gcaagtggtg caggtggtca gcacatcaat
     9121 aaaacagaat ctgctgtacg gatcactcac ttgcctacag gaacagtagt agctagtcag
     9181 gcacaacgtt cacaattgaa aaaccgtgag caagcgatgg ggatgttaaa agctaagctt
     9241 tatcaattag aagtggaaaa gaaagaacaa gaagcagctg cacttcgagg agaacaaaaa
     9301 gaaatcggct ggggttcaca gatccgttct tatgttttcc atccatattc tatggtaaaa
     9361 gaccatcgga caaacgtaga gacaggaaat gtccaagcag ttatggacgg tgaccttgat
     9421 ttatttattg acggctattt aaaaatgcat ctacaataag aacccattgt taaattgaaa
     9481 taaaattgta agattttgca agcatgttga aacattgcca tgctataata gctaaagact
     9541 gaaaaagata tggagagaac gccatgattg aaatgaagga tgtaatgaag aagtactcaa
     9601 atggtacgac cgctatccgc aatctctcaa ttgtcatcga acaaggagag tttgtctatg
     9661 tggttggtcc gtctggagcc ggtaaatcta cattcatcaa attgatgtat cgagaagaaa
     9721 aagcttcaaa aggaactttg aacgtcgcag gtcatgatct aatagcaatc aaaaaccgcg
     9781 aagttcctta tctacgcaga gaaatcggcg tggtgttcca agactataaa ttattaccaa
     9841 gaaaaacagt atatgaaaat gttgcgtacg ctatgcaagt tatcggcaga aaacctcggg
     9901 atatcaaaaa acgagtaatg gaagtgctgg accttgttgg tttgaaacac aaagttcgag
     9961 tctttcctag cgaattatcc ggtggggaac agcaacgtgt ctctattgct cgggcaatag
    10021 tgaatacgcc taaagtactt atcgcggatg aaccgacagg aaatctggat ccagaaaact
    10081 catgggaaat catgaaatta ttagatcgga tcaatgccca aggaactact gtcgtgatgg
    10141 ctacacataa cagtacgatc gtaaatacga ttcggcaccg agtaattgct atcgaaaacg
    10201 gccgcattat tcgagatcaa gcggaagggg aatatggata cgatgattag aacctttttc
    10261 cgccatttat tagaaagtat caagagtttg aaacgtaacg gctggatgac agttgcttca
    10321 gtcagtgcgg taacgatcac attgacttta gtcggtgtat tcatggcagt tatcatgaac
    10381 gcaactaaat tagctcaaga tatagaagga aacgttgatg tctctgtatt cgtagatatc
    10441 ggtacaaaag acgaagagat gaaaaagcta gaaaaagaat tgaatgactt agatcatgtt
    10501 aaaaaagttg aattttcaag caaagaacag gaattgaaaa aaatccaaaa cgaaatgggt
    10561 gattcctgga aattgtttga aggtgacaat aacccgttat atgatgtgta tgtagtaagt
    10621 gctactgatc cttcttatac aaaacaagta gcagaagaag cagcagattt gaagaatgtt
    10681 tctcgttctg attacggtgg agcttcctca gatcggattt tccaaatcgc tggtgcagtt
    10741 cggacatggg gattagttgc agcggcattg ctgctgttcg tagcgatgtt tctcatttcg
    10801 aacacgattc ggatcacgat tttgtctaga cagcgtgaaa ttcaaatcat gcgtttagtc
    10861 ggtgcaaaaa atggatatat tcgctggccg tttttccttg aaggtggctg gatcggtctg
    10921 atcggtgcga ttcttccgat tttgatcatc acatttggtt atgcatggtt tttccaattg
    10981 atcaatccat cattattacg ttcccattat tcattgattc atccacaaaa tatcgtttgg
    11041 aaaatcaatc ttttaatggt cggcattggt gtgatcattg gttcacttgg ctcgatcatt
    11101 tctatgagac gtttcttgaa attctaatac cacaatataa ataaaatgag aaagttggac
    11161 agtttcgttc cagctttctt tttttattgg ctaggagaat agaaggaagc ggcttttttc
    11221 cctttacttt aaaaaacaac ttgggtattc ttaagttggt agaagaagag gtgaagccgt
    11281 gaaaattatc gttgacaatt tttttacgat ccttttgatt atgaatattt tgctttcctt
    11341 tatcatcgtt tttcgagaaa gaaaggaaac tgctcaaaca tgggcatggg tgctcgtttt
    11401 gatgttcatt ccagttgtag gatttatttt atatattttt ttaggaagag ggatttccaa
    11461 agagaaaatc tttgatttga agatccaagg gaaaatcggt aagaacctgg aaattgaaga
    11521 agacaaacag gcattgatgc gagggcttta tcctcatccg cctactggac aagtagatgt
    11581 gaaacaattg atctatatgc tcacggtatt tgaaagtacg ctctatacga ctaaaaatga
    11641 aatcacttta tttacagatg gtcgtgaaaa attcgatgcg ctgattgaag atatcaagca
    11701 ggcagaagac catatccact tccagtatta tatttatcgc agtgatgcat taggtgaaga
    11761 agttcgagat gcattgacag aggcggcaag acgaggggta aaagtcagag tccttcttga
    11821 tgcatggggt tccacacaag tttcttccag cttttttcag aatttgaaaa aagcaggcgg
    11881 agaagtcgca tttttctttc ctctatttgt tccgtatatt aatcctcgga tcaattatcg
    11941 caatcatcgt aaaatcgtag ttattgatgg gaaaatcgga tatacaggtg ggttcaatgt
    12001 tggaaatgaa tatctaggat tggtaaagaa atttggttat tggcgagaca atcatttgcg
    12061 tatttacgga gaagcggtgt ataccttgca aaaccgtttt ttgatggact ggaattctca
    12121 gcatacaaaa gaagtaggct attctcccaa attttttccg tccattcact ctactggtga
    12181 tatagctgtt caaatcgtga caagcggacc agataccgaa catgaacaaa tcaaaatgac
    12241 ttatttgaaa atgatttcgt tagccaaacg ggagatattg atccaaacgc cttattacat
    12301 accggatgga tcgattcatg aagcactgaa gttggctctt ctttcaggcg taaaagttca
    12361 tatccaaata cctaacaaac cagatcatct tctcgtttac tgggcaacgt attctttcgc
    12421 tgcagagcta atcgagtatg gagcaaggat cgaaacatat gagaatggat ttattcatgc
    12481 aaagacgatg attatcgatg gtggcattgt ttcagttggc tcagcaaata tcgatgttcg
    12541 ttcatttcgc ttggactttg aggtcaatac actggtttat gatgaacgaa tggcttcacg
    12601 tgtaagaaaa gctttttttg aagactcgaa aatttctacg catctcacaa aagaaatgta
    12661 tgaaaaccga ggaataatca tcaagatgaa agaaggatta gcaagattga tttcgccgct
    12721 cctttaaaac ataaaaagga catgagaacc atcaacagat ggagatctcg tgtccttttc
    12781 taacggggcc atttctgaga gtacgtcaga aatggagaaa gcgtctcgca tgagcgaaat
    12841 taactttggg gtatctcttg ttttttgggg aaagcaagaa ataagctaat atatgctatt
    12901 aaaagagaca catttcaaag ccagtatagc atagaaactt ttaatgagat agtttttttt
    12961 acaattatga gaatttattt tgttatttaa tgattttgat tcgttttcat caagtaatga
    13021 aaatgataaa ataaacatac tttaggatta aaggagattt ctaatgaaaa aacttaaatt
    13081 attcttacca ataataggat tagctattct tttatcaggc tgttcaaagt ggattgatcg
    13141 aggagaatcc attacggctg ttggctcttc tgctttacag cctttagtag aaacagttgc
    13201 tgaaacttac cagacagaaa atcctggaaa attcatcaat gtacaagggg gcggaagcgg
    13261 aactggtttg tctcaagtac agtcaggagc agtcgacatc ggaaactctg atttgtttgc
    13321 ggaagaaaaa tcggggatcg atgcctcagc attagtcgat catcgagtag cagttgttgg
    13381 gatcactcct attgtgaaca aggatattgg cgtaaaagac atcagtatgg aaaacctgaa
    13441 aaaactattt acaggcgaga tcaaaaactg gagtgaagtc ggtggaaaag atcagccagt
    13501 agttatattg aaccgcgctt caggaagcgg aacccgtagc acgtttgaaa aatgggtatt
    13561 ggacggagaa acagctgttc aggcacaaga acaagattca agcggaatgg tgcgccagat
    13621 cgttgccgac acacccggtg caatcagcta tgttgctttt tcttatgttt ccgatgatgt
    13681 agatacgttg aatgttaacg gtgtagaacc gacagatgaa aatgtgacaa cgaacaaatg
    13741 gacgatttgg gcatatgagc atatgtacac aagagatcat ccaaaaaaac tgacaaaaga
    13801 atttttagac tatatcttat ctgatgaagt acaagataac atcgttggag aactaggtta
    13861 tatccctgtt tctcatatga aagtcgaacg tgattggcaa ggaaatatcg ttaaagagta
    13921 atctttacac aaaatttaca aacttagcta aacttttaca tgttattaaa accaaattga
    13981 tgttcaattt gtacactgag tgagtatgtt aaacctgatt aagggggagt catctgtgga
    14041 agatgtgaaa aaagtattat taacaaaatc aaaacgttca aaaatggaac agcgtggaaa
    14101 attcatcagt tttttatgta tcgcgctgat cgtgttggtt gtattatcta ttttttattt
    14161 tgtagctagt aaaggattgg ccaccttttt tactgataag atcaatgtat ttgattttct
    14221 gtttggtaca gattggaatc caagccaaat cggagcaaat gggaaaccaa tggtaggggc
    14281 attgcctatg atcacaggtt cgtttattgt gaccttttta tctgctatcg ttgccacgcc
    14341 atttgccatt ggtgcagcag tattcatgac ggagatctct cctaaaaacg gaacgaagat
    14401 cttacagccg gtcatcgagc tgcttgtagg tattccttct gtcgtttatg gttttatcgg
    14461 tttatcagtt atcgtgcctt ttgtcagaag tatttttggc gggactgggt ttggtatctt
    14521 agctggtaca tttgtgttgt tcgttatgat cttgccgact gtcacaacga tgactgttga
    14581 tgcattaaag gcagtgccac ggcactatcg ggaagcttca ttagcgcttg gtgccacaag
    14641 atggcaaaca atctacaaag tcgtgctacg agcagccgtg cccgggattt taacagcagt
    14701 cgtttttggt atggctcgtg cgtttggtga agccttagct atccaaatgg tcatcggtaa
    14761 tgcagcattg atgccaacag gtcttactac tcctgcttct acattgacgt ccatcttaac
    14821 aatgggaatc ggaaatacaa tcatgggaac agttgaaaat aatgtgttat ggtcattagc
    14881 gctgatcttg ctattgatgt cattattctt taatatcgtg atccgtatga ttgggaagaa
    14941 aggagagctg aaataatgaa cgcaaaacgc tcagataaaa ttgctacagg tgttttatat
    15001 atagtttcgg ggatcatcgt attgatttta gcagctttac tactttatat cttagttcgg
    15061 ggaataccgc acatttcatg gtccttcttg accgaacctt ccaaagccta ccaagaaggc
    15121 ggtggaatcg ggatccagct atttaactcg atttatttgc ttttgatcac gatgatcatc
    15181 agttttccta tttctttagg tgcgggtatc tatctttcag aatatgctgg aaaaaactgg
    15241 ctaacagatg tgattcgcac gtcgattgaa atcttgagct cgttgccttc tgtcgttgtc
    15301 ggtttattcg gtttcttggt tttcgttatt caattccagt atggtttctc gattatttct
    15361 ggtgcattgg ctttgactgt attcaacttg cctcttttga ctagaacagt tgaggagtct
    15421 ttacaagcgg tccattacac acaacgagaa gcaggattgg ccttaggttt gtcacgctgg
    15481 gaaacagtga taaaagtggt ggttccagaa gcgacaccag ggattttgac tggtgtgatc
    15541 ctaagttctg gaagaatttt tggtgaagca gctgcattga tctatacggc tggacaaagt
    15601 gcacctgcgt tagattttac gaactggaat ccattaagtg tttcaagccc gatcagcatt
    15661 ttccgtcaag ctgaaacatt agctgtccat atttggaaga tcaatacaga aggcacgatg
    15721 cctgatggtg tttctgtttc ggctggcgct tctgccgtgt tgattattgc tgttttactg
    15781 ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc gaaaaatgac ttcagcctaa
    15841 ggagaagaag caaaatgaaa aaatataact ggaatgaaac aaatgtcatc accatgccaa
    15901 aagagaaaga agtagcacta tatacggatg atctacatgt ctggtatggc gacaatgaag
    15961 caatcaaagg agtggatctg gaatttgaaa aaaataagat cactgcactg attggtcctt
    16021 ccggctgcgg aaaatctacg tatcttcgtt cgttgaatcg aatgaatgat gggatcgcta
    16081 atacgaaaat cactgggaaa atcatgtatc aaggcgtaga tattaatgcg cctcaagtgg
    16141 atgtttatga gatgcgcaaa cggattggca tggtttttca acgtcctaat ccgttcagca
    16201 agtcgattta tgacaatatt acatttgctt tgaaacagca cggcgaaaaa aacaaaaaga
    16261 aattagatga aatcgttgaa acaagcttga aacaagctgc cttgtgggac caagtaaaag
    16321 acagcttgga caaaagtgcc ttggctttat ctggcgggca acagcaacgt ttatgtattg
    16381 ctcgtgcgat cgcgatgaaa cctgatatct tgctgctaga cgagccggca agtgctttgg
    16441 accctatttc gacaggaacg gttgaagaaa cacttgtaaa cttgaaagaa gactatacga
    16501 tcatcatcgt gacgcataac atgcaacaag cagctcgtat cagcgactat actgcctttt
    16561 tctatttagg aaaagtaatg gaatatgaca aaactaacaa gatatttacc agaccaaaaa
    16621 ttcaggcaac cgaagattat gtgtccggtc attttggtta agacggaggc ctcaacatga
    16681 caaaagagat tatttcatct aaagatcttc atctgtatta tggaaagaaa gaagcactaa
    16741 aagggatcga tctaagcttc aatcaagggg aaatcactgc gatgatcggt ccttctggtt
    16801 gcggaaaatc cacttatctg cgttctttga accggatgaa tgacctgatt ccgaatgtaa
    16861 cgatcaccgg aagtgtttta tataaagaca aagacatcta tggtccaaaa acagatgttg
    16921 ttgaactaag aaaagaaatc ggcatggtat tccagcagcc aaacccattt cctttttcta
    16981 tctatgaaaa tgtcatctat ggattgaaat taaaagggga aaaagataaa aaagtccttg
    17041 atcaagtagt tgaagaaagc ctgaaggcag cgtctgtttg ggatgatgtg aaagacaaat
    17101 tgcataaaag tgctttatct ttatccggtg ggcagcagca gcgtgtatgt atcgcacgtg
    17161 ttttggcagt taatccggaa atcatcttat tggatgaacc aacaagtgcg cttgatcctg
    17221 tttctactgg gaaaatcgaa agtatgctgt tagaattaag agagcggtat acgatgatca
    17281 tcgtgaccca taatatgtct caagcttcac gtatttcaga taagacggca ttctttttag
    17341 atggtcactt gatcgaatac aatgatacga agaagatatt catgaatcca gaaaaacaag
    17401 agacagaaga ttatatttca ggtcgatttg gataaggagg aaacggaatg ctacgtacac
    17461 aatttgaaga agaattgctg aatctccata atcagttcta cgaaatgggg atgatggtga
    17521 gcagcgccgt gcacaagtca gttcgtgcat acatcaaaca cgataaaatc ttagcccaag
    17581 aagttatcga aaacgatata aatatcaata acatggaaac acgcttggag aagaaaagtt
    17641 ttgaaatgat cgcattgcaa cagccagtta caactgattt gcgtatgatc attacggtca
    17701 tgaaagctag ctcggattta gaacgtatgg ccgatcatgc ggtgtctgtt gctaaatcca
    17761 cgattcgcgt aaaagggcag acgagaatac cggaaatcga aaaagagatc tccgatatgt
    17821 ccgattatgt gaaaaaaatg gtggacaacg tcctagttgc ttatgtcaaa acagacgaaa
    17881 aagacgcacg tacgatcgcc aatatggatc aaagagtcaa tgactatttc aatcgaatct
    17941 ataatgcaac aatcaaaaat atgcaggaaa atcctgagac agtcatcagt ggaacagact
    18001 acttgaatgt tgccagttat ctagaacgga tcggagacta tgtgacaaac atctgtgaat
    18061 ggatcgtcta cttggctaca gggaaaatca ctgagttaaa tacgaaccac aatgagattt
    18121 tctaaactga agctgtgatt atgccgtttg tttaggaaaa gaggttgtga aatcagcggt
    18181 tgtgaaaaag ctgttctgca tcaagtaata agaacagtca aacaaaaaat agctcctcat
    18241 atttttcgtt tggctggact tattaccgca gatgcaagct tttgaacacc gtttattcgg
    18301 gaaagggttt gtgacaaaac atgtcacaaa cccttttcat catttcatag aaaggataaa
    18361 gaatgcaaaa aaaaattagc atcatcacta tattttttat ttgtgtgctt acttcatggg
    18421 ttattcagca tcaagccgct ttatatgaag tacctgtcat cgagatagaa aaagtacgaa
    18481 taaaagagga tcatcaattg attacaggaa aactgatgaa cacggatcaa aaaggagaat
    18541 cgatttattt atcggttcct tatcaaaaaa atcaaataga aacagcaaat ataaaagcga
    18601 gacaaatgta tctagtccaa atgcatggag atgaatggca gctagtagca aaaaaaaatg
    18661 atggctggct tttcttcttc acaagtatct tcattggcgc catgttgctt attggtggaa
    18721 aacagggtct atttgcttta ggaagtctca tcatcaatat cgttttactt ttacttttgt
    18781 tgctcatctt tactaaaaca gaagggattt ctctattaaa tatatctatc ctgttcgtcg
    18841 ttatagggat gattattagc atattggctt tagatggatg gaatagaagc agtataatca
    18901 aaatttcagc tgcagttacg agtgtttttt tagcattttt tatttgtctt ttgacaatga
    18961 atcattggaa agaccaagga ctgcgctatg aggaactgaa ttatctgaca cgaccgtatc
    19021 gttctgtttt tctttctagc gtactgattg gaacagctgg aggaacactc gataccgtta
    19081 tcacagtcat tgcgacatta gaagaattga ctaagcgaca accagaaata acagcgaaac
    19141 agttatggct ttcaggaagg caagtgggtc gagatgtgat tggctcgatg accaatatcc
    19201 ttttgttcgt ctatatcagt gggtcagttc catcgcttct tttatattta ggaaatcaat
    19261 ggtcgttcaa agaaacgata gaacagcatt tatcattgga atttttacgc gtcattgctg
    19321 gcagcttagg aatagtatta tcgatcccta tctcggtcct cttttttctg actgtaagga
    19381 ggctgaaacg atgaatgtac ttattatact tgttgggatt ttagcaatca gcgtgctact
    19441 tattggcggt acgcaaggta tgtatattct tttaggctta ttcatcaatt tagggatctt
    19501 tttcctgttg ttattcggtt atcatcagaa gtggccgata ctggcattgt cggtcatagg
    19561 atttttattg attgctgtgg ttatcttatt ttttatcaac ggttataatc taaaaatgcg
    19621 tgcagctttt gtgagtatac tgatcttttt attttgtttc ctactattgc ttcctatcac
    19681 tgatttttta gcaatccaag gttttacatc ggtagaacta gaagaattat ctggcttaga
    19741 taagacttta gcgattgatt ttagattact tacgagatcg ttgcttctga ttagtcttag
    19801 tggtgctgtt ttagatgcga gtgtagcaat ttctagtggg acattcgaag tatatcaggc
    19861 aaatccgcaa ttatcttttt accaattgcg ccattctagt ttcgctattg ctaaaaaaat
    19921 attggcgtca acagtaatga cacttctttt tgcattcatg ggaagttctt tagcgttgat
    19981 cctctggttt attgatttag acattccttt tcagcaagtg atcaatgaaa aatcatttgt
    20041 tctagaatat acgatggcta ttcttaccac attgtccgca ctacttgttc ttcctttaac
    20101 ctgcgtgact acagcatatc tctttacaaa aaagaaagaa caactcaaga tggagtaaaa
    20161 gttacaactt aagtcctatg acatttttac tgaatcagtg cataatatgg atgtaaggaa
    20221 aagcttgcaa acaattttgc ttaaggaggc aatttccatg aaagaaagag aacgtatttt
    20281 agatttagtg aaaaaaggaa tcctttcgac tgaagaaggc ttagatttat tagaaagcat
    20341 ggcaactgaa aaagacgaaa agcaaatcaa aaaagaagca gacaaggtaa ctgctagtca
    20401 taaagaaaaa gatcaggcaa gtcaattgat cgataaactt gaaaacggcg aagaagaaat
    20461 ttctgagcca gttgatccaa aggaaagaga aagacaagat caagaaaact tagaaaagat
    20521 cttagatgaa ctagcaactg aagcgaataa aacttcggcg cgtctagatg aagtaaatgc
    20581 tcaattcgct gacaataagg cagcacgcaa tgagaaacag gaagcattga tgcagctgaa
    20641 tacaaaagaa gaattaggcg agttgacaga agaagaatta gcacagcgtc agacgttaga
    20701 agcagaaatc aaagaattag aagctagtgg cgatacactg ttagaagaac aattgaaatt
    20761 agaagcagaa ttaaaagata tccgtaagaa tcaatggtct gagaaaaaag aaacgtttac
    20821 tgataagttt gaattgccag atgattggaa agatcaagca acagatacac tgaatcaagt
    20881 aggcgaaaaa atgtcagaag ctggatcaca gttaggcaaa ttcttgaaga aaacattcca
    20941 aactgtttca gaaacagtga atgacaacat ggaatggaaa gatgtcagct tacgtgtccc
    21001 tgggattgcg acaacgaaat ttgagcatga attttactat gaagctcctg cagcaagtat
    21061 ccttgatatc aaggcagcaa acggaaacgt gacgttaaaa acgtgggaca gcaacgatgt
    21121 gaaagttgaa gctaagatca aactttacgg aaaaatgggt gctgaaccat ttgaagcatt
    21181 ctctgaacgg agccagattg aggttaatga agatcatatc tccttccaaa ttccaaataa
    21241 acgtgtgcgt gcagaccttg ttttctactt acctaaacga gtatacgacc acgcagctat
    21301 taaacttcta aatggcaaca tcatgattga aacattggaa gctaaagata tttatacaaa
    21361 atctacgaat ggtaacatca tcgtggacca attaactgca acgatgctgg aagtagaagg
    21421 tgtaaacggc aatatcgata ttcgaaacgg gaacatctta gattctatta tcgaaacagt
    21481 aaatggtaca gtgacatttg gtgcgacagc agaaaacctt tctgtgtctt tggtaaatgg
    21541 tgatgtacgt cttacgatta aagaagataa tttgaaaaaa gtagaagcaa gttcagtgaa
    21601 cggaaatgtc aaagtcgctt taccagatgg catcggtttg gaaggacatg ctaaaacaag
    21661 tcttggaagt atcaacagcc gtctgtccaa ttacgaagta gttcgtgaga aaaaagaacg
    21721 gacaaaccaa atgttgcaat tccgtcgtgt ttcagatggt gaaatcgcgc aagtgcaact
    21781 ttcaacgaca acaggaagta tctatttaaa agatacagat aaataaagag gttgtgaaag
    21841 agatgttcag ccttgagcaa taagaagcga ttttgtaaaa tagcttcccc tattttacaa
    21901 aattgtgcct tattgccgat agtctatctc ttgaacaccg tttaccaaga gattatgaac
    21961 ctgccaatta tcgccgagtc acaaggaata atttcagaaa aagctcgtca cgttttatga
    22021 atatggtaat ttgtgaccga gaattttgca gaaagacacc gattaagaag aaggttgtca
    22081 caacgatttt cttcatttaa aatagtagaa cagaggtgag acatgatgaa aaaaaagttg
    22141 acaaaatcta ataaaaatgt tgttttgaca gggactttag caggaatagg agaatacatc
    22201 gggattgatc cgactgttat ccgtgtgctt tatgtattcc tgagcttagt tgcttttgga
    22261 agtccaatta ttttatacat tttattagct ttgattatcc catctgcgcg ttctgcacga
    22321 acaaactatg gacataacaa tgcatattat gaaaaaaaca attatcgtga acaaactcgt
    22381 gaacaaacaa aaaaacgcaa agaagcggaa aaaatcgatg atgacgaatg gagtgacttt
    22441 taatgtcgta ctttcaacgt attttagtta atacgttaac attcatatca ttggctgttc
    22501 tttttcctgc gatggtccat gtgagcagta tttggacggc cgttcttgcc agcttcgtat
    22561 tgtctgtgtt gaatatgttt gtcaaaccga ttttgacgat actgtccctg ccctttacat
    22621 tagtcacatt gggattgttt acgtttgtta tcaatgcagc gattcttaaa atgacctcct
    22681 tttttgtggg agcagaacat tttagttttt cttcattcgg tgcagcgttg ttagtagcta
    22741 tcttgatgtc gcttgtcaat gtaattgtca gcgaacataa cagtagaaaa taaaagaaag
    22801 ctgtgccgaa ctatcggcgc agttttttta tttagaatca ggaatgcgtt ttctattaca
    22861 ttggtaatta aataaaaacg ttttttttaa ttggtagtac ttttggctag acaatgataa
    22921 aatgatagag aacactctaa tagaaatgag gtagcatgaa tgtcagaagt tgtaaaagtt
    22981 agccaattag tgaaaaaatt atctttggaa atcgtgacag gcgacgagaa aagcttagac
    23041 cgtgtcatcg taactgggga catctcacgc ccaggactag agttgactgg ttattttaat
    23101 tattattccc ataatcgttt acagctattt ggcagtaaag aaatcagttt tgcggaacgg
    23161 atgatcccag aagagcgttt gatcatcatg agaagattat gtagtgaaga tatgcccgca
    23221 tttattattt caagaggatt accagctcca gaagaaatga tccaggcagc aaatgaaaat
    23281 gggatagctg tactgcgttc tcccatctca acttctcgtt tattaggaga attatccagt
    23341 tatttagatg gtcgccttgc ccctcgaaca agtgtccacg gtgttttagt ggatgtttac
    23401 ggattaggtg tactgattca aggtgacagt gggataggaa aaagtgaaac agcgttggag
    23461 ttgatcaaac gcggccatcg attgatcgca gacgatcgag tagatgttta tcagcaagat
    23521 gaattaacgg tgatcggaga accgcctaaa atcctggaac atctgattga aatccgagga
    23581 atcgggatca ttgatgtaat gaatcttttt ggtgcgagtg cggtacgtgg ctctatgcaa
    23641 gtccagttgg ctgtttatct ggaagcatgg gcaaaagata agaaatacga ccgtctgggc
    23701 tctgaagaca cgaccgttga aatcgcggaa gtgggcattc cacaaataaa aatccctgtc
    23761 aaaacaggac gaaacgttgc aatcatcatt gaagtagccg cgatgaattt ccgcgcacgt
    23821 acaatgggct ttgacgcaac gaaaacattt gaagaaagat taagtcgctt gattgaagaa
    23881 aactcaggca acgactagca aggagtgaca atatgttagc acaaatcaat cgagttgctt
    23941 ttgagcttgt agggattcct atctactggt atgcattgat catcgtatca ggtatcatta
    24001 tcgctatgtg gcttagcagc agagaggctg ttcgggtagg attaaaagca gatgatgtga
    24061 cagattttat gttgtgggga ttgcctatct ccattattgg cgctcgctta tattatgttt
    24121 tatttgatct tccccagtat atagctgatc caatccaaat attcaatatc cgttcaggag
    24181 gcttagctat ttatggcgga ctgattgcag gcgcgatcac gctgtatttt tttactaaat
    24241 atcattttat ttctttgtgg acttttttag atatcgctgc accaagtgta ttattagcac
    24301 aggcaatcgg tcgttgggga aatttcatga accacgaagc atatggacca gacacgacgc
    24361 gaagcttctt agaaagtctg catttaccgc aattcatcat tgataatatg tatatcgaag
    24421 ggatctatcg tcaaccaaca tttctttatg aatcactttg gagtttgatt ggatttattt
    24481 tgcttttgtt tttacgaaag aaaccacacc ttttgaaaaa aggcgaagtc cttttatgtt
    24541 atctaatgtg gtatagtttc gggcgtttct ttattgaagg attacgtaca gacagtcttt
    24601 actttttagg tgtgatccgt atttctcaat tattatctgc tgtgttattt gtcggtacat
    24661 tgattctatt tatttggcgc agaaaaaaag gaaatttacc ggattatgac cgctcattag
    24721 gaaaaaatca acaaattatc tgatttttag agcgttaaaa atgaaaaaaa tttcaggagt
    24781 ggtaaaatga attatagagt agcggtttta ggaccaggct cttggggtac tgctttggca
    24841 caagtcctcg ccgaaaacgg acatgacgtt cggatttggg gacatcgtgc ttcacaagta
    24901 gaagaaatca acacccaaca cacgaatcaa cgttatttac cagatcatta tctacccgat
    24961 tccatccaag cattcaccaa tatggaagat gcagtaaaag acgcagatgc ggtgctattc
    25021 gtcattccaa cgaaagcaat acgttctgtt gcaaaagaac tagtgcctaa attgaataca
    25081 aagccagtga tcatccatgc aagtaaagga ttagaacagg gaacacataa aaggatctct
    25141 gacatcttgt tagaggaaat cccagcagaa aaaagaaaag ctgttgttgt tctttctgga
    25201 ccaagccatg cagaagaagt agcaaaacat gacatcacta cgatcacagc tgcaagtaca
    25261 gatgcaaaag cagcaaaata tgtacaagag ctgttcatga ataattattt tcggatctat
    25321 accaatcctg atgtgatcgg tgtagagatg ggggcagctt tgaaaaatat tattgctatt
    25381 ggtgctggcg ctattcacgg cttagggttt ggcgataatg caaaggcagc aatcatgacg
    25441 cgcggattag ctgagatcag ccgtttaggt gtagcaatgg gtgccaaccc gctgaccttt
    25501 atcggattaa gcggaatagg cgatctagtc gttacttgca caagtgttca ttctaggaac
    25561 tggcgcgcag gcaacttgtt aggacaaggg cagcccctag aagaggtgct agccaatatg
    25621 ggaatggtcg ttgaaggtgt gaatactacc caagcagcga tggaactagc ccaaatgatg
    25681 gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc tgtatgaaca aaaagaaatc
    25741 aaaacagccg caaaagaaat catgttacgt gatggaaaaa aagaaaatga attctcatta
    25801 acaatttttt gaaggagcga atgtatatga aagttaaaaa agcagttatt ccagcagcag
    25861 gattaggaac aagattttta ccagcaacaa aagctatggc aaaggaaatg ttgccaattg
    25921 ttgataaacc aacaatccag ttcatcgttg aagaagcgtt gaaatcaggt atcgaagaca
    25981 tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga tcactttgat tcaaatattg
    26041 aattagaaac aaacctaagt gaaaaaggaa agaccgaatt attgaaatta gtagaagaaa
    26101 cgacagatgt caacttgcat ttcatccgtc aatctcatcc aaagggttta ggacatgccg
    26161 ttcttcaagc aagagccttt attggaaatg aaccatttgt cgttatgcta ggcgatgata
    26221 taatggaaga cgaagtacca ttgacacagc aattaatcga tgattacgaa cgtactcatg
    26281 cttctacgat cgccgttatg caagttcctc atgatgaaac ttcaaaatac ggaattatca
    26341 atccaggaga agtattggaa gatgggttat acaatgtcaa aaactttgtt gaaaaaccag
    26401 atccaagcaa agcaccaagt gatttagcta ttattggacg ttacttattg actccagaaa
    26461 tttttgatgt attgtccgag caacaaccag gagccggaaa tgaaatccaa ctaactgacg
    26521 caatcgatac attgaataaa acacaacgcg tatttgcccg taaattcaca ggtaaacgtt
    26581 acgatgtcgg tgacaaattt ggttttatga aaacaagtat tgaatatggt ctaacacatc
    26641 cagaagtagg tgcaccttta cgtgagtaca tcctgtcttt aggtaaaaca ttagaagcag
    26701 aaggaagcaa gatcgatcat aatccaaaag gaataacaga aatcaaagaa gaaaagaaaa
    26761 aataaccctt ttttctaacg agcttcagct gctaattgaa taaaaagtag ttgaagctcg
    26821 tttttatttg tttaaaaaac acgaaaatgc tatccttata gaaaagagag acaagaaggg
    26881 agtttgatca tgtcaggagg agaaattgca ggattgatcg cagcaatcgc ctttgcgata
    26941 cttgtcgttt ttatcgttcg tgtgttgttg caagtttcta aaactttaga aaaagtagat
    27001 cagactgtag cagaagcaaa tacgacgatc gaaatcgtca caaaagacgt agatcttttg
    27061 tctagacaag tagaaggctt actggtcaaa agtaatgaac tgctcttgga tatcaatgga
    27121 aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg atttaagtga aagtgtatca
    27181 gaacttaatc attctagtaa aaacatcgcg acgaaagtcg gcggaatcgg taaaacgaca
    27241 gctaaagcga cggttgccgg aaaagtcggt gggacagcga tgaaattttt taaaaacaaa
    27301 aaacatactg aaacgacagg aggaaaataa aaatggctaa aaaaggtgga tttttattag
    27361 gtgcagtagt cggaggcaca gccgcagcaa tcgccgcgtt attgttagca ccaaaatctg
    27421 gtaaagaact tcgggatgaa ttagctgccc aagcggatga tttgatggat atggcttcag
    27481 attacacaga tatggcgaaa gaaaaatcga ctgagttaac aggtattgca aaagaaaaag
    27541 cgcaagatct ttctcaacaa gcaggtgatc tagcaggtag tgtcaaacaa aaagtaagtg
    27601 aatcagcaga tgaagcagga agtgaatctg acgatatctt gttaaatctt aaagaacaat
    27661 cagctgactt atctaatcgt ttcaaaaaaa cggcagaaga tatgaaaaaa cctgcagatg
    27721 aattaggaag tattgctaaa gatacttcag aagatatcat gattgatgta aaagacgctg
    27781 ctggtaaagc aggagatact gtcaatgaag gaatgtcaga agcaaaacca gttgcagatg
    27841 actcaaaaga aatgatgaaa atggcaaaag aagatgcgaa agaagctaaa aaagaaataa
    27901 aagatacaat gacagattca tctacaaatc actaaaatca caaatttata gaataaaaag
    27961 gtgggagcgg actagctctc acctttttta ctcttatttt tctaaaacaa gtaattcttt
    28021 tggtgtagtc gtgaatggca cacagccatc gcttgtcaca tatacacagt cttcgatacg
    28081 tacccctact ttttcaggaa tatagattcc tggttcaaga gagaagcaca tcccttcttc
    28141 gattactaag tcatttcctt ctacaaggga agggaattcg tggacggttg ttccgatccc
    28201 gtgacctaaa cggtgattga aatattcgcc gtagccatag ctgttgatca cgtttcgtgc
    28261 gatttgatca agttcacctg ctgtcacacc tggacggacg gcttcttgtg cggcaagctg
    28321 tgcttcaaga gtgatgttgt agattttttc ttggaagtcg gttggttttt gataagcaat
    28381 tgtacgagta gcatcactgc aatagccatt ccaaacaacg ccaagatcga ataacacaag
    28441 ttcattcggt gaaatcttcg tatttcctgg tgtaccatga gggcttgcag catttgctcc
    28501 tgcaaggact aatgtgtcga aagacatttg tgaaacacct tgttttttca attggtattc
    28561 aatttctgca atgatcgctt gttcagtcac gccttctttc actgctttaa agccaatctc
    28621 aaatgcaaca tcggcccatt taccagcttc gatcagtttg tctttttcct ctagagtttt
    28681 gatcagttgt agacgttgga tgatggaagt tagatcagtg taagaatccg ttcccgtcaa
    28741 gatatcagag atagcatcga agcgctcgac agtcaaggcg ttcttttcaa tccctacatt
    28801 gccaggctga ccatagcgag cacgaagttc ttttccgatg atctcccaag ggttttcgct
    28861 gtctaaatag ccacggacat catacgccca actactgttt tgagcatctt caacttccaa
    28921 agcaggagtg aaaaggaaag gatcttgttc aagagggata aaaagagcta gtacacgctc
    28981 atgaggatca ctttcgtatc cggaaaagta agcgatgtgg ccagggtcgc tgatataagc
    29041 caattcgact tggtgttgtg ccatccattg acgtagttca ttgattttta cgttattcat
    29101 ttggcaacct actttctaaa ttaattttgt attcaataat tgtaccactg atttattaaa
    29161 agaccaacgt tctaaaaact gaaaaaagta aaaattttca tgaaaacaga gataaaacgg
    29221 ttgcaattct tgagattatt tgatattcta ttttagaaat gtaagacgta ttttcagaaa
    29281 acatataaaa tatgaatagg agaacacaaa tggaaaagca aacaatcact atctatgatg
    29341 tagcgagaga ggcaaatgtt tcgatggcta ctgtttcacg tgtcgtaaat ggcaatccaa
    29401 acgttaaacc agcaacaaga aaaaaagtct tggaagttat cgatcgcttg gattatcgtc
    29461 caaacgcagt tgcgcgtggc ttggcaagta agaaaacaac aacagttggt gtaatcatcc
    29521 ctgatgtcag caatatgttc tttgcttctt tagcacgtgg tatcgatgat gtggcaacaa
    29581 tgtacaaata caatattatc ttagcaaatt cagatggcaa cgaccaaaaa gaagtcaatg
    29641 tattgaataa cttactggct aagcaagtgg acggcgtgat cttcatgggt caccatatca
    29701 cagacgaaat ccgtggagaa ttttcacgtt caaaaactcc tgttgtatta gctggctcta
    29761 ttgatccaga tgaacaagta ggtagcgtaa acattgatta tgtatccgca acaaaagatg
    29821 cagtaaacaa attggcaaaa agcggcaata aaaaaatcgc ttttgtcagt ggtgcgttga
    29881 tcgatcccat caatggacaa aatcgtctga aaggctataa agaagcatta agcgaaaata
    29941 acctaccata tagcgaagga ttgatctttg aagcacaata caacttcaaa gatggtctat
    30001 ctctagtcga tcggatccat aatagcggtg caacagctgt ctatgtttca gatgatgaat
    30061 tagccatcgg tattttggat ggtttattag accgtggcgt gaaagtaccg gaagaattcg
    30121 aaatcatcac aagtaacaat tcactactga cagaagtagc tcgtcctcgt ttaacaagta
    30181 tcacacaacc attatacgat ttaggggcgg tatctatgcg cctattgacg aaaatgatga
    30241 ataaagaaga agtcgaagaa aaaacgatta tcttgcctta tggaatcgaa gaaaaaggat
    30301 ctacaaaata ataaaaggac aaccattcag aggttgtgtg cctgccgttt aactctgcgt
    30361 tacaaggagc aatttcaaaa aacagcatct catgtttgat gaatattgtg actcgtgatt
    30421 cggagttggc aggagaacac cgtttatttg ggaaaagggg ttatgacaaa attttgttat
    30481 aatccttttt ttgtgtctta aaaaagagag aaagtggttg aaattacatt tatcgtatat
    30541 aaaacaatcc gtgtatagat gctgcatttt gttcaccacc atcaaaaaaa taagggactg
    30601 tgacaatact attgtcacaa tcccttatta ctgtgtattc tattaaatca atcatctttc
    30661 tcatctgatt ctttagttgc ttgattcgct cttgcataag ctgaagcggt gttccagtaa
    30721 tctgtatagt tttcatcggt accgccaacg ccaaatttaa aggcgctttt cttcggtcca
    30781 tttttcgccc atagcgattc aactgttttt cctggtgttt ggatggattt tttattgaca
    30841 gtaacttttc caggtgtttg accagtaaat ccagatactt ttttcttcat cacatcagat
    30901 gataattcga atttctcatc agtgccaaaa atgtcaggtt ttgcttggta agcttgattg
    30961 atcagattgg caagataatt agccgaacgt atccttgctt tgctgtccat tccagtattg
    31021 tcatcgtgcc ctgcccagct actgatggta atagacggag tagagacaat tagccatgaa
    31081 tctcggtatt cattggtaga accagttttt ccgacccaat ctgcttttcc tagatttccg
    31141 tttaagcttg agatggcatc tttgaatggt gtagtgattt ttgcattgat cacactacgc
    31201 atcatgtcat tcatgatcga tgccgttgct tccgaataga tccggacaga gttgctttcg
    31261 tgtttataaa ttacatttcc tgcgttatct gtgatggaat cgatgatata gccttcttca
    31321 tagacgccgc cgtttgctaa agcctgaaat ccgttggtct gttgaagcgt cgtgacatct
    31381 actgtaccta aaggagcaga ttcaacgccc caattgtcat ttgccgggta gttcatcttt
    31441 gccaagtagt gctcgtaagc atattgtttc gatccaccgg aattcaacac atcttgatat
    31501 aagtgataag ccgggatatt gttggaccat tctagtgact cacggacggt ttggaaggtg
    31561 ttcgatcctt cgttagtagc attgacgatt ttctcgccag cattttctcc ttcttgccaa
    31621 gttgttgcat aatctgatac acgggattct gatccgataa gccccatgtc gattgcaggt
    31681 ccataaacaa gaactggttt gattgatgag ccagcttgac gttctgtatc gaaggcatgg
    31741 ttattttgat tctgactgta attccgtccg ccaacgaatc cgtagatgcg gccagttctg
    31801 ttatccatca atacatttcc ggtttctact tgactgccag caccatcatc taacatgtat
    31861 ccataatttt caacgccatt ttgcaatgca gcatagacat ctttatcgat tgtcgaatgg
    31921 attgtatatc ctttattttg gatcgtttgc tgtgcctttt gataataagc gtcactgacc
    31981 gatgaatcag acatatcagc gttgtctttt tcagccaatt gttgtgcaat gattcttgtt
    32041 gcttcattca tcacagtata atacaagaat tctctgtcag tctgttctgc gatttgttgt
    32101 ggtagaaagt cttttgtaag gtcataggct tttgcttctt cgtattgttc tttcgtaatt
    32161 tggccttcac gatacatact gaataaaaca aaatcttttc tggcaaggcc ggcagaaaga
    32221 tcttctttta gcgcgcctgt gttcgtgtat ggactatacg tgatcgggct ttggggtaat
    32281 ccagcgatat aagcagcttg cggcagcgtc acatcttttg catctacacc aaagattcct
    32341 tttgcagctt cctggactcc agcaatgttt tgtcccttat tattacgacc aaatggtgag
    32401 acattcaaat acgtagcaat gatctcatct tttgaaaagt atttttctac ttgtgcagaa
    32461 agaatgatct cgtttgcttt acgtttgaat gttgtttcat ctgtaaggat ctgctgtttg
    32521 actaattgct gtgtcaaagt tgatccacca gaagagccaa taccagtagc ttcagatact
    32581 aatgctcgta aaacagcttt aggaacgtaa ccttggtgtt tatcgaagta ttcatcctct
    32641 gtgctgataa ttgcagtttt caataaaggt gaaatcttat cagatgaaat agtcgtgcgc
    32701 atcagatctg tctggatttt tgatatctct gtattatctg cataaacaat ctttgacgtt
    32761 tctgtaatgt ttcctagttc tgtctgcagt gcttttttag tcggcaattg tgtgtcttcg
    32821 acgagatagg caaaatagcc agcgcctatt ccaataccta gagctgcgaa catcaaaaga
    32881 agaacagtaa taaaaacagt caatgattga aaaactctaa aaataatctt aggaacaaac
    32941 catttttctt tttttcttct attctgtttt tttcgagtat ttttgcgtgg caaaagggtc
    33001 acctcaacta atggttttat ttcgctaatt atagcaaata atagaacaaa aaaacagaca
    33061 aggctgtttt tggcaggaat ttctttttta aaagagttaa aacgtgagaa aatgatcttt
    33121 attataaaag agttcttaag cttttcttta gagaaaattt cagttactat ggagaacagg
    33181 gcaaagaaaa atcaatcatt ttctttgtat atcatcttac caatatatag gaattgttag
    33241 taacaattgc ctttcaagga ggaatccatc catgttatta ggctcacacg tcagcatgag
    33301 cggaaagaaa atgctgttag gagcagcaga agaagcagcc agttatggat cttcgacttt
    33361 tatgatctat acaggcgccc ctcaaaacac tagaagaaaa gccatcgaag agatgaatat
    33421 tgatgccgga aaagcgtaca tggcagaaaa aggtctaaca aatatcgtcg ttcatgcccc
    33481 ttatatcatt aatttgggga atacgatcaa accagaaatg tttccttttg ccgtccaatt
    33541 tctgagagaa gagattgttc gtgcggaagc tttaggcgct actcaaatta cacttcaccc
    33601 aggtgctcat gtaggggcag gaacagaagc cggaattgct agaattattg aaggcttgaa
    33661 tgaagttttg acaaaagaac agacggcaca aatcgctttg gagacaatgg caggtaaagg
    33721 tacggaaatc ggtcgaacat ttgaggaatt agcgcaaatc atcgatggag taaaattgaa
    33781 tgaaaaatta tccgttactt ttgacacctg tcatacgaat gatgccggtt ataatattaa
    33841 agaagacttt tcaggtgtaa tggaagagtt tgataagatc atcgggttag atcggcttaa
    33901 agtactccat atcaatgatt ctaaaaatcc gcaaggttcg cataaagacc ggcacgccaa
    33961 tattggtttt ggaacaatcg gattcgacac gctgaataag atcgtccatg atccaatgtt
    34021 tgacaatatt tcgaaaatct tggaaactcc ctatgtaggc aaagataaaa agaaagctta
    34081 cgcaccatat ggaaaagaaa tcgccatgtt caaagcgcaa acattcgatc cagaagtgtt
    34141 tcctgagtta ttagcagaag aagtgatact ctaagatttt ctatctgaca gcagcaaaaa
    34201 aggaccaggc acattgcctt gtcctttttt gtttaagaaa caaaggagta ctttttgcag
    34261 tctttttcat catgcatctt ctcccccctc tttttaaatt tagacaaaca aggtatactg
    34321 gtagaaaagt aaaaaaacga aaagtttcac aagcaagtgg caaaacgtga ttggaaggac
    34381 gaaaagatgc tgaacacttt aaaacccttg atcaaccaac taaaaaagaa acatacatac
    34441 tttattcttg gcatagccct attatttaca attattcgtt tgtatttata tagtaaagca
    34501 atctatggaa cagacatgtc ggcaacctat gatgaccagc tgctgattcg ctatgctcaa
    34561 aatattttgg acggtaaatg gctaggggag tatacgacta caacactttc caaaggaatc
    34621 tcctacagct tatttttagt acttgcacat aaactgcatc tttcttatgc agttttgttc
    34681 ggcatgttca gtatttttgc cagtatattg gttgcactcg cgtttaaacc gattgtgaag
    34741 aacaagtatc ttcttttaag tatctatttt tattttttgt tttctccgat tagttttaca
    34801 catgaatatt ccactcgaac atatcgtaat gccctcgtga ttccggctgt tgagatcatt
    34861 atcgcttgtt tattagctat ttattaccgc aaatacggtt ctagcagaaa attgctgcca
    34921 tggtttttag ggctttctgt cgcttttcct ttcttttggt tcattcgcga agattctatc
    34981 tggcttcttc cttttactgt agtagctttg ggtatcagtt tcgtccagat cttgctaggg
    35041 caagactttt ctttcaaaga agtaaagaag ttaaaaaaac gactctttct tctctcgaaa
    35101 cgggaatggc tgaaactttc attgttttta cttcctatag ttggcttttc catcgtctat
    35161 caatggatcg aatataaaaa tgagacaaca tacggtattg caaccatcaa tgaccgttcc
    35221 agtggagaat ttggacgttt gatgaaacaa ctgatccgaa tagatgatga aacgaacttg
    35281 aataaagaaa acagtgagat atgggtctca catgctgcac taaataaagc attagccgca
    35341 tctcctacat tccatactgt ttctacccgt attgacgaat tatatacgac tcacgcgtgg
    35401 actcaaggtg gaaaagtaaa agaattaccc ggagatatca tcttttgggc attgcgagat
    35461 gtggtaagcg aatctggtta ttaccaaaac aatgcaaaag aaaccaatga tttttggaaa
    35521 aaagtcaacc aagaattaga agaagcctat cacaagggaa caatcaaaaa gaaaaaagag
    35581 ttctatctga tgggatcagg agatggaaag acgatgagtg atctgccaat cgtattcgat
    35641 ttccttcaat caggatacgc ttatgcatgg cgatataaag atttccatca aggaggaaat
    35701 tatagtttcg gaacgcaaga acaagtcaaa caggctcaag aattattaaa ccttccgcta
    35761 atggagaatt ggatcgatag taatgaaccg agaacaccta atttccaatt aacaaaatca
    35821 gcaaaagtcg cgaatgtcat cataaaaatt taccaagttc tggctattcc cgtgctgatt
    35881 gcttcaatag cagggatatt acttatgata ctaggaaccg tatttgcaaa agctgatcgt
    35941 gcctattata gttcctcttt gattgtggtc accggattaa tactgacaca attggttttt
    36001 cttttcggtg tctcatggtt ttgttcttat gcgccagaaa aaaaggacta ttttttaagt
    36061 gtctacactg gtgcaggcgt gccattaacg caattcatcg tagtaacaag catgctagtt
    36121 ttcagtaaat taaatctgaa aaaagcttta tcttctaaat aaaaagaccc ttggtagcta
    36181 gatttttaac ctaactatca agggtctttg tgtggaaaaa tcagttttta ttcgatttct
    36241 tctaccgttt cctgcaaaag ctgttcatat tttttgtttc ctttatataa atcaccaatc
    36301 gtccatgcca acaggagaag gacaagaaca atgattacat aagcgatgtg atcagccaat
    36361 gctagttgag cggaagtggc ttgctcgccg aagaaacttt cgatctgtcc tggtaatcct
    36421 cgtaagttca agtaagttag actaatcaca gagaaccagc caagaagttt tacgatgaat
    36481 gaatttttaa atcggctacc catctccgct tggctgttgg tcatcatcag taaaggaatc
    36541 atcgaaaacg gcaacgcaaa agccaagaac acctgggatt cattcattaa gttattcaaa
    36601 gcggcatgtt cttcgatcgt tccttttgta cttgtaaaca aaacacaaat caatacagga
    36661 atcacagaaa gcaatcgtgt gaccagtctt cttagccaaa tcgggatacg catatggatg
    36721 aatccttcca tgatgacttg accggtcaac gtaccagtaa tagtagagtt ctggccagaa
    36781 gcaagaagtg ctacagcaaa taaagtggac aagataccag attttgctac agcaatcaaa
    36841 ataccattgc tcatagctga tggatcagat aaagcttcat acagaccaaa aaaggaagga
    36901 tcttggacag aaccggtttt gaagacagcg acacccatga tcaatagcag tgcattgact
    36961 agaaaagcca ttgacaactg gatattagaa tcccaagtag aaaaacgtac agcttgtgct
    37021 acatcttctt cgttttggtg atcgatcttc cgagtttgtg aaactgctga atgaagatat
    37081 aagttatgcg gcatgactgt tgcaccaata atccctaaag ctccagtcag cggagtttcg
    37141 ccgccgatga ccggtgagtc tgagaatgta tgactgttag ggataaagcc agcaaataaa
    37201 ctgtgccaat ctgggttaga caagccaact tgatagacaa aaacgaataa aatgacgaaa
    37261 attaagcaga caaccagtgc ttcgatctta cgaaacccga cttttgtcag tagtaagaga
    37321 acgaaaacat ctaatacagt aataaagacg gcaatcacca atggaatatc aaaaagtaag
    37381 tagagggcga ttgctgcacc aatgacttct gcaatatctg ttgccataat ggctaattca
    37441 gtcaagatcc ataaaatgac acctaatgtc ttacttgtgc gtgctcggat tgcctgtgcc
    37501 aaatccattt ggctcacgat tcctaatttt gctgccatat actgtaaaag catagcaatc
    37561 agactagaca tcaaaataac tgacatcaat aaatattgga aattttgtcc tccggtgatc
    37621 gaagtagacc agttacccgg gtccatatac ccaactgcta ctaacgcacc aggtccagag
    37681 taagcaagaa gtgttttaaa aaaacttttc ccctttggta catcaactgt tccatttact
    37741 tcagctaaag ataaaccatt tgtatgttca ataagtcgat gcttatgaaa ttgtttattg
    37801 gattgattat tcaagaaaaa accacctttc ctatttatct acgattacat tctacacctc
    37861 aaaaagaaaa aattaaagaa aaagattcgg tacgcctaat tttttttcat ggcgcgtctt
    37921 gctagatgac gaaagaaatt ttgttctcct ctaaaaaaac cactattttt tgcatgaaac
    37981 taaagaatac gcctttcttt tttcaataaa ctttagtaaa ataagtcatt gagagcaatt
    38041 cggaaataga aaggagagat ttatatgtta tcaacaggta ttgtcgtact tattgctatt
    38101 attgctttgc taatcggcgc agtaggtggc tttttcctag cgcgtaaata tatgaaagac
    38161 tatttagaaa aaaatcctcc cgttaatgag gacatgttgc gatcaatgat gatgtcaatg
    38221 gggcaaaaac catctgagaa gaaaattcgc cagatgatgc agcaaatgaa gaaccaaggg
    38281 aaaaaatagg ttctgaccca acgacttttt acgggctgat tttttcagtc cgttttttat
    38341 ttcgtaattt gttagactat tcagattatt cagcaaaaaa gacaagcaca gacaatccta
    38401 ggctcttgat aaagacaaag gaggagtaaa atgtcgattt ttagaaaatt aggctggttt
    38461 tttcgtcagg aaaagaaaca ttatttaatt ggggttttct cgctattcgc agtggccttg
    38521 gtacagttag ttcctccaaa agtgattggg atcatcattg atgagatcgc cgataaaaat
    38581 ttatcaataa agatcatttt cgggtggatc ttcctattga ttatcgctgc ttttttacaa
    38641 tatctttttc gttatatctg gcggacgaat atttggggaa gtgcagctcg attggagaaa
    38701 gaacttcggc agcagttatt caaacatttt acagaaatgg acaatctctt ttaccaaaaa
    38761 tatcggactg gggatttgat ggctcacgca acgaatgatt tgaatgcgat ccagaatgta
    38821 gcaggagctg gtatcttgac ctttgcagat tcagtgatta cgggcggaac aacgatcatt
    38881 gcgatgattt tgtttattga ttggcgctta acactgattg cgctgattcc tctgcctttg
    38941 ctggccgtca cttcacgagt gctggggtca aaactccatg atgcttttcg tgattcgcaa
    39001 gaagcttttt ctagtatcaa tgataagaca caagaaagta tcactgggat caaagtcatt
    39061 aaaacatttg gacaagaaaa agaagatcta gcggattttg aagaaaagat tgaagatgca
    39121 attattaaaa acaaacgagt caatttttta gactccttat ttgatccatt cattaccttg
    39181 attatcggtc tgtcttatgt tgcaacaatt attttaggtg gaaagtatgt cttggaaggc
    39241 acgatctcga tcgggcagat ggtctcattt attagttata tcggtatgct tgtttggccg
    39301 atgtttgcga ttggtcgatt gttcaacgta ttggaaagag gaaatgcttc ttatgatcgc
    39361 gtcaatgaac ttttgcatga aaagacgcat atcgtggaac aactgggtgc agtgcagacc
    39421 ccggcaaaag gcgagctgaa tgtagctatt tcctctttta attatccgaa agaagaatca
    39481 ccagcattag aacaaatcaa gtttgcgatc caagaaggag aaacattagg tattgttggg
    39541 aaaactggtg cgggaaaaac gacgatcatc aaattactga tgcgtgaata cgatgattat
    39601 caagggaaaa tccaatttgg cggacgttcg atcaaagaat attcattgaa tgctttatta
    39661 ggatcaatcg gctatgtgcc tcaggatcac ttcttgtttt cgatgaccat tcgagataat
    39721 atccgttttt ctaatccaaa actttcagaa gaagaagtag agcatgcggc ttctttagca
    39781 tttatcaaca atgaaatcaa ggaattacca aaaggttatg ataccttagt tggagaacgt
    39841 ggagtctctt tatctggcgg acagaaacaa aggatttcga ttgctcgagc attgatcaaa
    39901 aatcctgaat tattaatatt agatgatgca ctctctgcag tagatgccaa aacagaagaa
    39961 gctatcttgc ataatttaaa agaaataaga aaagagaaaa caactatcat tgcagctcac
    40021 cgtttaagta gcgtgatgca tgcaaaagaa atcattgttg tcgatcaagg gaaaatcatt
    40081 gaacgaggaa cccatcatga gctgcttgca ctaggcggct ggtatgaaca aatgtgggag
    40141 aaacagcaat tagaagcgaa gatagaagga ggcgaggcct gatggatgaa aattatcaat
    40201 cggaatggac aaaatcgatt ccacttaaag aacagaaaaa gatcatcaaa cgactgatga
    40261 agttcgccaa accttttaga agaacgttca tcgttgctat cttatttgcg tttgctttgt
    40321 ctgtgatcaa tgtcctattg ccaaggatca tccaaacttt tatggatgat catctggcta
    40381 aacagtcggc tacgacacaa gttattttgt tttttgctgg tatgtactta tttggagtca
    40441 tagtaaaaag tatcatttgg tttttccaat ggtatttgta ttccatggcc tcattgaaga
    40501 cctatcagta tattagggta aaattatttg aaaagctgca tacactaggt atgcgttatt
    40561 ttgatcagac accggcagga tcaaccgttt ctcgagtgac gaatgacaca gaaacgctat
    40621 ttgaattttg gtacgtcttt ttgatggtat tgactgggat ctttgcagtc gtttcttcat
    40681 tccttgccat gtttcaaatc aatggagaaa ttgccttgtg gactttggtg tttttgccga
    40741 ttttaggaat cgtcatctgg tattaccaga aattcagttc tcgtatttac cgaaatatgc
    40801 gggaaaaatt aagtcagctg aacacgaaat tgaatgaatc gatttccggc atgcgtatta
    40861 tccagcagtt tcgccaagaa gctcgtttag ctaaagaatt tgaacaagtg aacaatgagt
    40921 atttagacac gcgttatgcg atgatcaaaa cgaattcgat tctgttaagt ccgatcatca
    40981 attttctata tgcattggcg attgcgttgg tgttgacttt gtttgggatc gatgcgttgc
    41041 attctcccgt agaagttgga ctgatctatg catttacgac ctatgtccag ggatttttta
    41101 atccaatgtc tcaaatgatg gactttttga gcatcttcac agatgggatg gttgctggta
    41161 gtcgtatctt gaagattatg gatacggaag aatatgcacc acaacaaaac cctggagcag
    41221 atcaaatgat tacttcaggt aagattgagt ttcgcaatgt aagcttttct tatgatggag
    41281 agcacaatgt attaaacaat atctcttttg ttgctaatcc gggagagacc gttgcattag
    41341 tgggacacac cggaagcgga aaaagctcga tcatcaacgt attgatgcgg ttttacgaat
    41401 tttatgaagg tcagatattg attgatgatc atgacattcg agaatatcca atggaagagt
    41461 tacgtaagaa aatggggctg gtcttgcaag atgctttctt gttttatggg gatatcgcag
    41521 gaaatattcg gatgatgaat ccagctatca cagacgaaca gatacgccat gcggctgagt
    41581 ttgttcaagc aaacaaattc atcgatgaat taccaaaagg atatcactca aaagtgatcg
    41641 aacatggtgc cagctattct agtggacaac gacagctgat cacttttgcg cgaacaatcg
    41701 tgactgatcc taaaattcta gtattggatg aagcaacagc aaatatcgat actgaaacag
    41761 aaggattgat ccaagaagga ttggcaaaaa tgcgtcaagg acggacaacg attgcgattg
    41821 cccatcggct gtcaacgatt cgagatgcag aattgatttt agttttagac aaaggtcata
    41881 tagtagaacg cgggaatcat gaaacacttc tagcgcaaaa tggtttgtat gcagatatgt
    41941 acaaactgca aagcagtggt atataaaaga tgttgtgaca gtagaatttt gacctaagca
    42001 ctagagtgaa aataaaacat agcttctcat atttttcatc tgtgagagtt agtgtcgtag
    42061 tcaccagatt taaaaaaaga ttcgacagct agtcagaagt gcctaatagc cactaagaaa
    42121 gcgttgacaa aagcatttgc tttcgataat ataaagaaaa cgatacaact tggatagtga
    42181 ttgcctgatg caagctaaac ctgatgagtc tcccatggtt tcatggacgg ctttttgggt
    42241 ttttcttttt gttttggata cagccctata catcatggaa aggagcaaaa catgctgatt
    42301 gagaaagtac tgaataacaa cgtcgtaata accagggatg aaagaaatca ggaaatgatc
    42361 gtgatgggaa aaggtttggc attcaaaaag aaagtgggtg acccgatttt tgactcatgc
    42421 atcgacaaaa aatttcagtt gacaaatgac gggctttcac aacagttcca agaattggca
    42481 caagcgattc ctcttgagta cttagaaatt tcttgtgaaa tcattgaata tacaaaagag
    42541 gctctacaag tccagttgaa tgattctgtg tatattgcat tgacggatca tttgtatacg
    42601 gctatcgaac gtcacaaaat gggaatattt gtaccaaatg tattgctgta tgatatcaaa
    42661 caactatttc ctaaagagta tgccatcggg aaaaaaacag tgaagaaaat ctcggataaa
    42721 taccacatca agttatcaga agatgaagcc ggatttatcg cattgcattt agtcaatgca
    42781 caaggcattc aaagccagca agacaatacc tacctgttta ctataacaat acaggatatt
    42841 ctaaatatcg tccgctatta ctataagatt acatttgatg aagattcagt ccattttttt
    42901 cgattcgtca cacacttacg tttttttctg acacgcgtcc tagatggaga aaaatcagaa
    42961 gtgggagaag tttctgagga attgtttcag gttattcaaa aaaaatataa ggatacgttt
    43021 ggttgcgttg agaaaatagc agacttttta gccgagacgt tcaactacca gatgtccacg
    43081 gacgaaaaaa tgtatttaac gatccatact gcgcgtttag tgcatgagaa tcgttgtaaa
    43141 taaaaaaagg aggataaatt aacaagaacg tatgaaataa gggtagtgac attcagttgg
    43201 ctaaaccttc cactaataaa ttggagggaa tagaaatggg aaaataccaa gaattagcga
    43261 gaaaaatcgt agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca cattgtatca
    43321 caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata
    43381 tggacggtat tgtaacggta atgcgagcgg gagggcagta tcaagttgtc attgggaatc
    43441 atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa ccttactttt gatgaggctg
    43501 tttcaacaga aaaaatgaga ccgtttgata tgcttattga tggaatatcg ggctgtttcc
    43561 agccattcct tgctatccta gctgctggcg ggatgattcg tggactgaca gcattccttg
    43621 tcttcttagg cgcgtttgac cgcggttctg gcacatttgt tatgtttgat aatatcggtg
    43681 attctgtatt ccaatttatg cctgttatta tcggtttaac agctgcccgt aaatttaaag
    43741 taaatgaatt cgtagggatg ttgcttggcg cagcattaat gaatcctagc ttgtcgctgg
    43801 aagctttgag tggtgcagca gaagcaccac taacgaccat attttctggc actatttttg
    43861 aagcacctat ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt
    43921 ctgtcattcc cattatcttt attgtgttgt tagctagcca aattcagaaa ccaattaaaa
    43981 agttagtacc tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa
    44041 tgcctttagg ttttttactc gttggaccag tattcacatt cgcaactgat attttaatgg
    44101 ctggatttga aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct
    44161 tctggcaaat tctggtgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc
    44221 aattttctgt gaatggttgg caaaatatta tgacacctgt tgcagtagtg tcttttggac
    44281 aaactgcagc acttaccgca ctctacttta agcttagaaa tccaaaagac aaagcaattg
    44341 caataccagc cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta
    44401 cattaccacg taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt
    44461 atagtgggtt aatgaattta acttcttgga atcaaggtgg actaggcatc tttacaattc
    44521 ccaactatat tagaccagat ggtgatttaa cagatgtgat taatgtattg attgggatcg
    44581 ccattgcgat ggtggtatcc tttacgctga cttttttctt ttggaaggat gaagcaggtg
    44641 aaacagatga tatacagaaa aaatcaggta aagaaattgt aaaaacaccg atacaaggac
    44701 aaatcgctcc actaaatgca gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag
    44761 gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatga
    44821 ccctgttccc gacgaaacag gcaattggaa tggtttctga aacagggctg gaattgctga
    44881 ttcatgtcgg attggatact gtgcagttgg aagggaaata ttttgaaagc ttagtgcagc
    44941 aaggataaag agtgaaacaa ggcgatctgt tgctacgctt cgacaaaaag aaaatcgaaa
    45001 aggaaggata cagtttagaa acacctgtga tcgtcaccaa ttacacggat tatttggata
    45061 ttttggaaaa ccaaggggag acggtcgggc cagacgatgc acttatcacc gcactgacct
    45121 gaacggttag gtgaagaatg gactaggctt aactattctt tttattccac ttctttattt
    45181 tcaaaattga aaggctcttt catgatggct gagctgatag ggcgtttatc gaatttttga
    45241 ccgacagaaa ttagatggac taaattctgt tgataatttt ctgcaaaaac acaactatat
    45301 aacacgtcta gaagataaca aattgatgta ttgatcgtaa aattaccaat ttttgaatac
    45361 agtcgctcgc gtgtcgtaag tcgcaaagcg gctgtactca ttgcagatag cgttgaatca
    45421 ccgatactgg tcaaagctaa aattggaaca tgattttgtt tcaatatttc tgcggtacga
    45481 aggatcacat tgttttcacc cgtataagaa atcagaatcc cgcacatatc actcgtgcaa
    45541 ttgtacgctt catagaatga ttcaccaaaa gtggtgacaa tcgtgacatc cattttgatg
    45601 cgcttcattt tcagcatgaa atcttgagaa atcaaagtat tcgagttact ggcgaaaatc
    45661 acgatttttt ttgcttgaag cattagttgt ttggcttttt gcagttggtc atgatgaatg
    45721 agagaaagtg tatcatgaat cgtcatctgt tctaaagaag ccattttatt tgcgatcgtc
    45781 ataataccgt ctgtggtgga aaaaggaaga ttcgcatcta cgtcttgaaa attcccttca
    45841 agataggctg tttcagctaa aaaagcatta cgaaaactga cccagccatc aaaacccatt
    45901 ttttttgcta cacggatgag tgtagagggg tggacataac atcgctcagc aatcgcttgg
    45961 atggtcattt cgccaagtgc tggagaatcg cttaagagaa actggatcac ttcattttct
    46021 gaaggagaaa aggagatttc tttgattttt tcacgtaata acatgtagat accttcttct
    46081 tattttttta cgtaaattat aacttttttg aaaatgattt gtaaacggat ttatactata
    46141 taacttaaaa tagacatttt ttgacaagat atttacaaaa atccactaga aaacagtaaa
    46201 ttttgtaaat gttactggaa agaaacagat ttctattgtt tgttttgttt acaaaaaaga
    46261 taatgaaagt gtaaaaaagt aaaggagtga gaacatggca aaaggaatca aaatcgctac
    46321 gattggtgga ggcagcagtt acacccccga gttgatggaa ggatttatca aaagatatga
    46381 ggaattaccg atacgagaaa tctggctcgt agatgtagaa gaaggaaaag aaaagcttga
    46441 gatcgttagt cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag tcaagatcta
    46501 tgcaactctg gatcgggaag aagcattgaa agatgcggat tttgttacga ctcagtttcg
    46561 tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc cctagttatt atgggatgat
    46621 tgggcaagaa acaaatggtg ctggcgggat attcaaagct tttcgtaccg ttccagtcgt
    46681 tttggcaatc gttgaggaca tgaagcgttt atgtccaaat gcttggctga tcaatttcgc
    46741 gaacccaagt gggatggtga cagaagcagt tatcagatat ggcaaatggg ataaagtgat
    46801 tggattatgc aatgtgccag tgatggcgat gatgacagaa ccagcaatgc ttggcaagaa
    46861 aaaagaagag ctgatctaca agtttgccgg tctaaatcat ttccattggc acaaagtagc
    46921 agataataaa ggaaatgacc gcacggcaga attgattgat cgtttatacg atgaagagaa
    46981 tggattaccg aaaaatatct ttgatatccc gtttttcaaa gaacaattaa aacaaatgaa
    47041 catgatccct tgtggctacc accgctatta ctatcgtgaa gaagaaatgc tggaacatgc
    47101 tttagaagaa tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga
    47161 gctatttgaa ttatacaaag atccagaatt agaccataaa cctgaacaat tgcaaaaacg
    47221 aggtggcgct tattattcag acgctgcctg tgaaacgatt gcttcaatct atggaaataa
    47281 agaaacacag atcgtggtat ctacaaagaa tgatggagct gttcctgacc tgccggcaga
    47341 ttgtgtagta gaagtcactg cctatgttgg cgcacaagga gcgagaaatg tagcgtttgg
    47401 ttctcttcct gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg aattgctgac
    47461 gatcgaagca gctgtgactg gtgattatgg gacagcttta caggcgttta cgatcaatcc
    47521 acttatacga tcaggaaaga cagcaaaacg gatcatggat gagttgttta ttgcacacca
    47581 gcagtattta ccgcaattca aggagacaat cagccgatta aaagaagaag gaatcgaagt
    47641 tcaagatgaa ctggcaagag aattatcagg taatacacat ataatggagg aagtataatg
    47701 ggattcagaa aagatttttt atggggcggt gctacagcag ccaatcaatg tgagggcggt
    47761 tatgatgaag gtggacgcgg actagcaaac gtagatgttg tgccaatcgg aaaagaccgt
    47821 tttcctgtta tcaccggaaa gaaaaaaatg tttgaatttg atgaggatca tttttatcca
    47881 gcaaaaattg caatcgatat gtatcatcgt tataaagaag atattgcttt gtttgcagaa
    47941 atggggttca agacttatcg aatgtccatc gcctggagtc gtatttttcc aatgggggat
    48001 gagaaagagc cgaatgaagc tggcttgaaa ttttatgagg atgcctttaa agaatgccaa
    48061 aagcacggaa ttgagccact tgtgacgatt acgcattttg attgtccaat gcatttgatc
    48121 aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa tctgtgtcga
    48181 gtcatcttca atcgctataa aggacttgtc aaatattggc tgacattcaa tgagatcaat
    48241 atgatccttc atgcaccatt catgggcgca gggctgtatt ttgaaaaagg agaaaatgaa
    48301 gaacaaatca aatatcaagc ggcacatcat gagttggttg caagtgccat cgcaacgaag
    48361 attgctcatg aagtagatcc ggagaacaaa gtaggctgta tgctggcagc aggagcatat
    48421 tatccataca cttgcaatcc tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat
    48481 tatttcttca ttgatgtcca gtcacgagga gaatatcccg cttatgcatt gaaagaaatg
    48541 gagcgaaatg gcattcagat cgaaatggaa ccaggggatg ccgcattgct gaaagaacat
    48601 actgttgatt ttatctcttt ttcttattat gcttcccgag tttcgactac cgacaacgaa
    48661 ctcttggaac agactgcagg aaatatcttt gcttctgtca aaaatccata tctgcaagca
    48721 agtgaatggg ggtggcaaat tgatccatta ggcttgagaa taacgatgaa tgatatttat
    48781 gatcgttatc aaaaaccatt gtttattgta gaaaacggct taggtgcagt cgatacgcca
    48841 gatgaaaatg gttatgtgga agatgattac cgaatcgatt acctggcagc gcatatccag
    48901 gcgatgaagg atgcagtaga actagacgga gtcgatctat taggctatac gacttgggga
    48961 tgtattgatt tagtatcagc tggtaccggt gagatgaaga aaaggtacgg attcatttat
    49021 gtcgatcgtg ataaccaagg aaatggcaca ttagcccgtt caaagaaaaa atcattcaac
    49081 tggtataaaa aagtaattga ttcaaatgga gaaaatcttt cgaacgattg atcaatgatt
    49141 tataaaagtc tattgttttt cagcggattt ttcgagtata ataaagctat tcgagaaacg
    49201 caagcagaac aatagacttt ttttcgcatt ggctgtaaag tatgcttact tgattaagta
    49261 aaggagaatc tacatatgta tgatgtaatc gttattggcg caggtcctgc tgggatgact
    49321 gctgctttat atgcttcacg ttcaaactta tccgtattga tgatcgaaca aggtgcccct
    49381 ggcggccaaa tgaacaatac ggcagaaatc gaaaattatc ctggattcga ttcgatcatg
    49441 ggccccgatc tagcttataa gatgtatgaa agtgtctcaa gatttgggac agaaaatgct
    49501 tatggaatcg ttcaagatat caaggatcac ggcagccata aagaagtcat ctgtgaagat
    49561 aaaagttacg aaggaaaaac agtcattatc gcaacaggat gtatccaccg taaattaggc
    49621 gtacctggcg aggaagaatt tgctggacga ggcgtttctt actgtgcagt ctgtgatggt
    49681 gcgtttttcc gtaacaaacg tctagtagtc atcgggggtg gggactcagc agtagaagaa
    49741 gccatctatt tgacacaatt tgcttctgaa gtagtgatcg tccaccgtag agatgaatta
    49801 cgtgcacaaa agatcatcca agaccgtgca tttgcaaatg aaaaaatttc ctttatgtgg
    49861 gatacagtcg ttgacgaaat cgttggaaac gaaatggtag tgacaggtgt gaaagcacgt
    49921 aatgtcaaaa ccggcaatga atttgattta gcagcagacg gagtattcat ctatgttgga
    49981 ctggatccat taactgcacc ttttagagaa gcaggtttga cgaacgaagc tggctggatt
    50041 ccaacagaca acgaaatgaa aacgaatatg ccaggcgttt ttgccatcgg agatgttcgt
    50101 gaaaaagatt tgcgacaaat cactacagct gttggtgacg gcggtattgc tggacaacaa
    50161 gtgtataaat acttggagtc tttagaagaa acaacagcat aagtttaaaa agagtagaag
    50221 aacgggttca attgttcttt tactcttttt tttcgcgtga aatttactga atctgattta
    50281 tgcgaacacg tccaacacct tttgataaaa gaatcctttc ttgatttaag cgttttttgg
    50341 tcctcatact ttcatatcta ataagtgaat gctatactta ttaagaacca tatttcagaa
    50401 agaggtgtta ttttcatgtc ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat
    50461 ttagaagaaa acttaaaaaa acaattaaat gatctgggtg aagatcctga aaaacttgaa
    50521 gatgcttttt atgcaccgtt ggaattcggt actgcaggta tgcggggaat cttaggacct
    50581 gggatcaatc gaatgaacat ctatacggta agacaagcaa cagaaggact agcacgtttc
    50641 atggatacac aaggtgaaga aacaaaacgc cgaggtgtcg ctatcgctta tgactcacgc
    50701 catcagtcac cagaatttgc aatggaagca gccaaaacac tagcacatca caatatcccg
    50761 tcttatgtat tcgaaagtct gcgtccaaca ccagagcttt cttttgcagt tcgtcatttg
    50821 aagacgtttg cgggaatcat ggtaaccgca tctcacaacc cagcggctta caatggatat
    50881 aaagtctacg gagaagatgg cggacaaatg cctcctgcag acgcagatgc attgacgaaa
    50941 tatgtccgtg aagtcagcaa tccgctaaaa gtcgaagtat tatctgacga agaagctaaa
    51001 catagcgggt tgatcacgat catcggagaa gaagtggacg ctgcttatct agaagaaatc
    51061 aaagcagtga cgattgatcg tgaattggtt gaaacaatgg ggaaagattt gaaacttgtg
    51121 tacacgccac ttcatggaac agggaaaatg ttgggcgaac gtgcgttgaa acaagctgga
    51181 ttcgaacaat tcgtactagt tcccgaacaa gctgtggcag atccagattt cactacggtc
    51241 aaatcgccaa atccagaaga acattcagct tttgaatatg ctatacgact tggagaaaaa
    51301 gaaggagctg acttgctgat tgcaacagat cctgatgcag accgtttagg cgcagcggtt
    51361 cgtttgccag atggttctta tgaagtattg actgggaacc aaatcggagc attgtttgct
    51421 caatatattt tggaagcaca tcaatcaaga ggcactttac cagaaaacgc agcggtattg
    51481 aaatcaatcg tttccagcga attgcctaca gcaattgcgg aacactacgg tgctaccatg
    51541 ttcaatgtgt tgacagggtt taaattcatt gctgaaaaaa tccaacaatt tgaagaagac
    51601 cattcacata cttttatgtt tggattcgaa gaaagttatg gttatttagt taaacctttt
    51661 gttcgcgata aagacgcgat ccaagcattg cttttattag cagaagtagc agcttactat
    51721 aaaaaacaag gcaaaacgtt gtatgacggc ttgcaaacga tttttgaaac ctatggctat
    51781 ttcgcagaaa aaacgatttc tgtgacaatg agcggacaag aaggttctgc gaaaatctct
    51841 gctttgatgg aaaaattccg taaagaagca ccgacagaat ttgccggagc aaaagttatt
    51901 caaacagaag actttaaaga attgacgaga acagaccgtg aaggaaacgt agaaaaacta
    51961 acaacaccgc catccgacgt attgaagtat acattggaag aaaatggctg gatcgctatc
    52021 cgcccttcag gaactgagcc gaaaatcaag ttttatattg gtgtaaaagg aagctctaca
    52081 gaagacgcag ccgaaaaagt agcctcttat gaagcgatta tcaaaaagat gacagaagaa
    52141 taaaaaactt gagactgatg gaaaaagtgg taaactttag aagtcagtct tttttcaagt
    52201 tggaggtgaa tggattgcca gacacgcaaa aatcaaaagt agagaaagtc agtcaattat
    52261 tcaaaatgtt gagtgatccc actcgtctta aaattctgct atatctgaaa gacggggaac
    52321 aaaatgtgac agcaatcacc caagcagtgg aaatggagca gtctgctgta tcgcatcaac
    52381 tacgtttatt aagagaaaat catgtcgtca agtctcatcg cgagggaaaa gcgattttgt
    52441 atagtcttga tgattctcat gtactggata ttttgaacca gacactcaaa catgtggaac
    52501 atcaataacg ataggtgaaa ataga
//
LOCUS       DAPCMT010000004        51604 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPCMT010000004 DAPCMT010000000
VERSION     DAPCMT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 51604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.004"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            12..740
                     /locus_tag="RD743_000168"
     CDS             12..740
                     /locus_tag="RD743_000168"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDU0562030.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            740..994
                     /gene="purS"
                     /locus_tag="RD743_000169"
     CDS             740..994
                     /gene="purS"
                     /locus_tag="RD743_000169"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDU0562031.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            996..1667
                     /gene="purQ"
                     /locus_tag="RD743_000170"
     CDS             996..1667
                     /gene="purQ"
                     /locus_tag="RD743_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDU0562032.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1668..3890
                     /gene="purL"
                     /locus_tag="RD743_000171"
     CDS             1668..3890
                     /gene="purL"
                     /locus_tag="RD743_000171"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDU0562033.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3875..5314
                     /locus_tag="RD743_000172"
     CDS             3875..5314
                     /locus_tag="RD743_000172"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDU0562034.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5337..6389
                     /locus_tag="RD743_000173"
     CDS             5337..6389
                     /locus_tag="RD743_000173"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDU0562035.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6386..6964
                     /gene="purN"
                     /locus_tag="RD743_000174"
     CDS             6386..6964
                     /gene="purN"
                     /locus_tag="RD743_000174"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDU0562036.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6961..8496
                     /gene="purH"
                     /locus_tag="RD743_000175"
     CDS             6961..8496
                     /gene="purH"
                     /locus_tag="RD743_000175"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDU0562037.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8512..9786
                     /gene="purD"
                     /locus_tag="RD743_000176"
     CDS             8512..9786
                     /gene="purD"
                     /locus_tag="RD743_000176"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDU0562038.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9908..10822
                     /locus_tag="RD743_000177"
     CDS             9908..10822
                     /locus_tag="RD743_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDU0562039.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10955..12520)
                     /locus_tag="RD743_000178"
     CDS             complement(10955..12520)
                     /locus_tag="RD743_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDU0562040.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12513..13964)
                     /locus_tag="RD743_000179"
     CDS             complement(12513..13964)
                     /locus_tag="RD743_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDU0562041.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13978..14994)
                     /locus_tag="RD743_000180"
     CDS             complement(13978..14994)
                     /locus_tag="RD743_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0562042.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15214..16302
                     /locus_tag="RD743_000181"
     CDS             15214..16302
                     /locus_tag="RD743_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDU0562043.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16356..17882
                     /locus_tag="RD743_000182"
     CDS             16356..17882
                     /locus_tag="RD743_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDU0562044.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17884..19089
                     /locus_tag="RD743_000183"
     CDS             17884..19089
                     /locus_tag="RD743_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0562045.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19346..20323
                     /gene="guaC"
                     /locus_tag="RD743_000184"
     CDS             19346..20323
                     /gene="guaC"
                     /locus_tag="RD743_000184"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDU0562046.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20587..22011
                     /locus_tag="RD743_000185"
     CDS             20587..22011
                     /locus_tag="RD743_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDU0562047.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22472..23581
                     /gene="pdhA"
                     /locus_tag="RD743_000186"
     CDS             22472..23581
                     /gene="pdhA"
                     /locus_tag="RD743_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDU0562048.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23584..24561
                     /locus_tag="RD743_000187"
     CDS             23584..24561
                     /locus_tag="RD743_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDU0562049.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24586..26229
                     /locus_tag="RD743_000188"
     CDS             24586..26229
                     /locus_tag="RD743_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDU0562050.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26237..27643
                     /gene="lpdA"
                     /locus_tag="RD743_000189"
     CDS             26237..27643
                     /gene="lpdA"
                     /locus_tag="RD743_000189"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDU0562051.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            27805..28080
                     /locus_tag="RD743_000190"
     CDS             27805..28080
                     /locus_tag="RD743_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDU0562052.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28086..28853
                     /locus_tag="RD743_000191"
     CDS             28086..28853
                     /locus_tag="RD743_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDU0562053.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29021..30850
                     /gene="typA"
                     /locus_tag="RD743_000192"
     CDS             29021..30850
                     /gene="typA"
                     /locus_tag="RD743_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDU0562054.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31111..32883
                     /gene="recQ"
                     /locus_tag="RD743_000193"
     CDS             31111..32883
                     /gene="recQ"
                     /locus_tag="RD743_000193"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDU0562055.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            32997..33299
                     /locus_tag="RD743_000194"
     CDS             32997..33299
                     /locus_tag="RD743_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDU0562056.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            33302..34465
                     /locus_tag="RD743_000195"
     CDS             33302..34465
                     /locus_tag="RD743_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDU0562057.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            34491..37919
                     /locus_tag="RD743_000196"
     CDS             34491..37919
                     /locus_tag="RD743_000196"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDU0562058.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            37940..39064
                     /locus_tag="RD743_000197"
     CDS             37940..39064
                     /locus_tag="RD743_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562059.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39054..39491
                     /locus_tag="RD743_000198"
     CDS             39054..39491
                     /locus_tag="RD743_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDU0562060.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            39475..39813
                     /locus_tag="RD743_000199"
     CDS             39475..39813
                     /locus_tag="RD743_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDU0562061.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            39932..40492
                     /gene="rsmD"
                     /locus_tag="RD743_000200"
     CDS             39932..40492
                     /gene="rsmD"
                     /locus_tag="RD743_000200"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDU0562062.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            40489..40980
                     /gene="coaD"
                     /locus_tag="RD743_000201"
     CDS             40489..40980
                     /gene="coaD"
                     /locus_tag="RD743_000201"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDU0562063.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            40970..42013
                     /locus_tag="RD743_000202"
     CDS             40970..42013
                     /locus_tag="RD743_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDU0562064.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42172..42900
                     /locus_tag="RD743_000203"
     CDS             42172..42900
                     /locus_tag="RD743_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDU0562065.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43016..43261
                     /locus_tag="RD743_000204"
     CDS             43016..43261
                     /locus_tag="RD743_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDU0562066.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43258..43476
                     /locus_tag="RD743_000205"
     CDS             43258..43476
                     /locus_tag="RD743_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDU0562067.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            43612..44151
                     /locus_tag="RD743_000206"
     CDS             43612..44151
                     /locus_tag="RD743_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDU0562068.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44144..46417
                     /locus_tag="RD743_000207"
     CDS             44144..46417
                     /locus_tag="RD743_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDU0562069.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            46455..47501
                     /gene="holA"
                     /locus_tag="RD743_000208"
     CDS             46455..47501
                     /gene="holA"
                     /locus_tag="RD743_000208"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDU0562070.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(47590..47844)
                     /locus_tag="RD743_000209"
     CDS             complement(47590..47844)
                     /locus_tag="RD743_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDU0562071.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48244..48513)
                     /locus_tag="RD743_000210"
     CDS             complement(48244..48513)
                     /locus_tag="RD743_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562072.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(48983..49438)
                     /locus_tag="RD743_000211"
     CDS             complement(48983..49438)
                     /locus_tag="RD743_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDU0562073.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(49576..51504)
                     /locus_tag="RD743_000212"
     CDS             complement(49576..51504)
                     /locus_tag="RD743_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDU0562074.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        16951 a         8885 c        11292 g        14476 t
ORIGIN      
        1 gagagacgta catggagaag aaagaacttt tgtatgaagg aaaagccaaa aaaatatatc
       61 ttacttctga tgagaacttg atttggatag aatatttaga tcaagcaact gcgttgaatg
      121 gcgcgaggaa agaccagata agtggaaaag gagaattgaa taatcaaatc acaagtgcaa
      181 ttttccgtta tctgactcaa agaggaataa aaaatcattt tattgaacaa ttgtctaaaa
      241 ccgaacaatt agcaaaaaaa gtacaaatta tccctctgga agtggttatc cgaaatattg
      301 cagcaggaag tttctcaaaa cgtctaggtg ttcccgaagg taaagaattc atcaaaccaa
      361 tcattgaatt ctactataag gatgaccagt tggatgatcc atttatcaat gaagagcata
      421 tctatttctt acaacttgca aaaccggaag aaatcagtga gctgaaagcc caagcgttgg
      481 ctattaatga aattttgacc gaattatttg ctaaggtcgg tttggtttta gtagatttca
      541 agctagaatt tggccggtcg aatggacaaa ttttgcttgc ggatgaagta acaccggata
      601 cttgccgatt atgggatgaa cgtacgaatg aacatatgga caaagacgtt taccgcagag
      661 atctaggtga tttgatccca gtctatcaag aagtatataa ccgtctatcg gaactaactg
      721 aagaaaagga gcgagtatga tgtatttcgt aaaagtatat gtaacctata aagattcagt
      781 gttagatcct caaggggagg cagtaaaaaa agcagtccat cgtatgggat atgaaacaat
      841 cgaagatatc cgaataggaa aatactttga gatacaactt gctgatgcag aagaaccaat
      901 cgatacagtg attgaaacga tttgtgacaa gctgcttgca aatgtaaata tggaaacata
      961 ccgatatgaa atcaaaaaaa tggaggaagt ataacatgaa atttgcggtc atcgtttttc
     1021 caggttcaaa ttgtgacgca gatatgttat gggcagttcg cgagatcatg ggcgctgatg
     1081 ccgaatttgt acggcatgat gagtcctctt tagaaggatt tgatggggtt ttactgccgg
     1141 gaggattctc ctatggtgac tatttgagat gcggtgcgat tgctcggttt tctacaatca
     1201 tggaagaagt gattcgtttt gctgaagaag ggaaaccagt attcggtaca tgcaatggat
     1261 tccagatatt gacagaagca ggacttttgc caggtgtact gagacgaaat gagtctttgc
     1321 attttatttg caaaactgta ccattgaaag tcaaccccaa gacggccttt acttcagagt
     1381 atgaagccga cgaaataatc tatcttccag ttgctcatgg ggaaggaaat tatttctgtg
     1441 acgaagaaac acttcaagaa ctaaaaaaaa ataatcagat tgcctttact tataatgaca
     1501 acatcaacgg cagcgtggaa aacattgcag ggattacgaa caaagcaggg aatgtgttag
     1561 gaatgatgcc tcatccagaa cgtgcagtag aagacttgct aggatcagaa gatgggaaac
     1621 ggttctttgc ttccatttta aaaaacttcg gaaaggtgac cgtatagatg aaaacattga
     1681 aagaaccgac agctgaagag atcaagaaaa aaagaatcta tgccgaatgg ggattgactg
     1741 atgaagaata tcgaatgatc gaagaagata tcttaggaag aatgcctaat tatacggaaa
     1801 ctggactgtt ttctgttatg tggagcgaac attgttccta taaaaattct aaaccggttc
     1861 tacgcaaatt tcctactagt ggacctcatg tgttgcaagg ccctggagaa ggcgcaggca
     1921 tcgtcgacat cggtgatgga ctagcagttg tcttcaaagc agaaagtcac aatcatcctt
     1981 cagccgtgga gccttatgaa ggagctgcga caggagttgg cggaatcatc agagatattt
     2041 tcagcatggg ggctcgtcca attgcgttgc tagattcatt gaggttcggc gaattagata
     2101 atccaagaac gaaatattta ctagaagaag tagttgcagg tatcggcgga tacggcaatt
     2161 gtatcggcat accgactgta ggcggcgaaa ttgcatttga tccttgttat gaaggaaacc
     2221 cattagtcaa tgcaatgtgc gtaggtttga ttgaacacaa acatatccag aaagggcaag
     2281 ccgctggcgt cggtaattcg atcatgtatg taggtgcaaa aacaggtcgc gacggtatcc
     2341 acggagcaac ttttgcctca gaagaattta atcaagaaga agaacagcaa cgttctgctg
     2401 tacaagtagg cgatcctttt atggaaaagc tgctgcttga agcgtgtttg gaattaatct
     2461 tgcatcattc ggattctcta ataggtatac aggatatggg agcagcagga cttgtttcat
     2521 ctagtgcgga aatggcttca aaagctggct caggacttat attgacatta gatgaagtcc
     2581 cacaaagaga aactgggatg acaccttatg aaatgatgtt atcggaatca caagaacgga
     2641 tgttgatttg tgttaaatca ggagaggaag aaaaaatcca agaactattt cagaagtatg
     2701 atctagatgc agtcactatc ggtaaagtaa cagacgacgg gcaatatcgg ctatatcatc
     2761 aaggagaaga agtagcaaat ctgccagtcg atgcattagc tgaagatgca ccagtttatc
     2821 ataaagaaat gaaagaacca gctcgaatcg tagaatttca gcagttagct ccttatcagc
     2881 cagttatcga agaacctgga caagtattgc ttgatctgct gcagcaaccg acgattgctt
     2941 caaaacgttc aatctacgaa acttatgatt cacaagtaca aaccaataca gttgtccgtc
     3001 caggaagcga tgctgctgtg atgagggtta gagggacgaa taaagcgtta gctatgacca
     3061 cagactgcaa cgcgcgctat ctgtatttgg atccaaaaat cggaggacaa atagcagtgg
     3121 cagaagctgc tagaaatatt attgctagcg gtggtaagcc cttggcgatc accgactgcc
     3181 tgaactatgg ttctcctgat aaaccagaag ttttttggga attatcgact tcagctgacg
     3241 gaattgctgc tgcgtgtgaa acgttaggaa ctcctgttat ttcaggaaat gtctctttat
     3301 ataatgaaac ggatggtcaa gcaatctatc caacaccaat gattggtatg gtcggattga
     3361 ttgaggatca taaacatatc actactcagg aattcaaaaa atcaggggat ctaatctata
     3421 tattaggtaa gacgtttgca gattttgatg gcagtgaatt acaaaagatg cagcttggaa
     3481 gaatcgaagg tgtgatccga aactttgatc tatccattga aaagagaaac caagagttag
     3541 tattgacagc catccaaaac ggattgattg aaagtgctca tgactgttca gaaggtggct
     3601 tggcaattgc actggcagaa tcagctttca aacatcagct agggatatct gtacagtttg
     3661 aattgtcctc tgctcaatta tttgcagaaa ctcaatcaag atttgtgttg acagtcgcac
     3721 cggaaaataa gacacgattc gaagaaatga tgggagatgc tgctgtttta gcaggtaagg
     3781 tgacggatga agcgataatc gaaatttcag caactgacgg tcagatcaaa atagaaactg
     3841 cagtagcaag aaagtgctgg gaggatgcaa tcgtatgtct tatgaagtga agagtttaaa
     3901 tgaagaatgc gggatcttcg gtgtttgggg tcatccagac gccgctagtg tggcatactt
     3961 cgggctccat agtcttcagc atcgaggaca agaaggtgca ggtatcgtgt ccaatgatcg
     4021 aggaaagctt aaggggcatc gagatttggg gctgctttct gaggtattca aagatcaaag
     4081 aaaattgcag cagttaacag gagaagctgc aatcggacat gtacgttatg caacagcagg
     4141 caatggaagt gtggacaata ttcagccttt tctattcaaa tttcatgatc agtcgatcgg
     4201 cttagcgcat aatggtaact tgatcaatgc tcgttccctt agaaaagagt tagaggaaaa
     4261 tggggcgatc ttccattcta actccgatac ggagatattg atgcatttga tccgacgaag
     4321 tgaagaaaca gattttttaa ataaattaaa aagtgctttg aatgaagtca aaggtggctt
     4381 tgcctattta ttaatgacag aaacggcaat gattgccgct ttggacccaa atggttttcg
     4441 tccattagca atcggacaaa tgaaaaacgg agcatacgtg attgcctcgg aaacttgtgc
     4501 attagaagtt attggggcac gttttataag agatgtccag cctggtgaga tagtgatcat
     4561 caatgacgaa ggaatccaga ttgattgctt tacgaaggac acgcagcttt ctatttgttc
     4621 gatggaatat atctattttg ccagaccaga ttcaaacatc gcagggatca atgtgcatac
     4681 tgcacggaaa aatatgggac ggaatttagc gaaagaatcg ccagtaaaag cagatatggt
     4741 cgttggcgta cctaattctt ctttgtcagc cgcaagcggt tatgcggaag ctagtggaat
     4801 cccttatgaa atcggattag taaaaaatca atacattgcc cgaacattta tccagccaac
     4861 gcctgagcta agagagcaag gtgttcgaat gaaattatct gctgtacgag gagtagtgga
     4921 aggtaaaaag gtgattatgg tcgatgattc aattgttcgg ggtacgacaa gcagacggat
     4981 cgtccatctt ttgaaagaag caggggcaaa agaagtccat gtgaggattg cttctcctcc
     5041 tttgaaatat ccatgttttt acgggattga tattcaaacg cgtaaagaac tcattgcggc
     5101 aaatcacacg attgatgaaa tcaaagaatg tatcggtgca gattctttag gttttttaag
     5161 tgaagagggt ctgatcgaat cgatcggtct ggaaaaagat gctccgtatt caggattgtg
     5221 tatggcttat ttcaacggag attatccgac gccgttatat gactatgaag aaaaatatca
     5281 ggaatcacta aaagaaaaag tatcgttctt ttaaaagcag accaagaagg gagtatatga
     5341 attcaatggc aaatgcatac gcaaaagctg gcgtagatgt cgaagctgga tatgaagtag
     5401 tagaacggat caagaagcat gtcaaaaaaa cggaacgttt aggtatgatg ggcgctatag
     5461 gaggattcgg cggttgtttt gatttatcgg tctttcaatt aaaagagcca gtccttgtct
     5521 cggggaccga tggcgtgggg acgaaattag cagtggcaat cgaagcagac aaacacgata
     5581 caatcgggat cgactgcgta gcgatgtgtg tcaatgatat cttagcacaa ggcgcagagc
     5641 cgttatattt cttagactac ttagcaacgg ggaaaaatga tccgttacgt ttggaaaatg
     5701 tagtaaaagg tgtagcagat ggatgtgttc aaggaaatat ggcgctgatt ggcggagaaa
     5761 cagctgaaat gcctggtatg tactcaggta atgattatga tctagccgga tttgctgtag
     5821 ggatagctga aaaatctcaa ttattgacag gagaaacgat caacccagga gatgtgttgc
     5881 ttggaatagc atctagcggg attcattcaa atgggtactc tcttgttcgc aaaattttct
     5941 ttgagcagca gcagttttct ttatcatcaa aactgccaga attgaaaaaa acacttggcg
     6001 aagaattgct tgagccaacg aaaatctatg tttcttcctt gcttccgctg atcaaagaaa
     6061 agaaaataaa aggagctgca catatcactg gtggagggtt tgtagaaaat atcccgcgaa
     6121 tgttaccaga atctatggct gcacaaatca aacttggctc atggccgatt cttccaatat
     6181 tcggctgttt acaaaaatat ggtgaaattc ctgagatgga gatgtatgaa atcttcaata
     6241 tgggcatcgg tatggtcctg gcagtcgaag aatcgaaagt aaaagaaata gtacacgaaa
     6301 ttgagcaagc tggcgaaaaa gtttatgaaa tcgggaaagt gatcgccaaa gaaaaacatc
     6361 gtgtggtgtt tataggagaa gaacgatgag aatcgctgtt tttgcttctg gaaacggaag
     6421 caactttcaa gcgctagccg attatctatc gaaaaaagga ttggaatctt cgattgactg
     6481 gttgttttgt gatcagcctg aagcatatgt tttgaaacgt gcaactgcgt tgagtgtacc
     6541 ggcagattgt ttttcaccta aagaatttga ttcaaaaaaa gagtatgaag aagcaatttt
     6601 gcataaattg aaagaaaaaa agatcgattt gatcgtatta gctggatata tgcgaatcat
     6661 tggaccggta ttgctgaaaa attatgataa aagaatcatc aatattcatc cgtctctttt
     6721 acctgccttc cctggtttgc atggcatacg tgatgccttt gaagctggag taaaagaaac
     6781 gggtgtcaca attcattata tcgaccaagg tgtagatact ggaccaatca tccggcagga
     6841 aaaagtgaga attgaacagg aggacacgtt cgattcttta gaagaaaaaa tccatagagt
     6901 agagcatcga atctatccag aagtgattag tgaaattatt gaaaatggag gctatctgaa
     6961 atgacaagag cattgatcag tgtatcagat aaaactggga ttgtagcatt cgcccaaggt
     7021 ttgattgaac aaggagtaga aatcatttca acaggaggaa caaagcatgc gttggataaa
     7081 gctgggatca aaacgctttc gatcgaagaa gtgacagggt ttcctgaaat gatggatggc
     7141 cgtgtcaaaa cacttcatcc taaaatccat ggtggtcttt taggaagacg cgatctccct
     7201 agccatatgg aggcaatgga aacggaaaat atccaaccaa tcgactttgt ttgtgtcaat
     7261 ttatatcctt tcaaagaaac gattatgaaa ccagatgtcg aaatagcaga agctattgaa
     7321 aacattgata tcggtggtcc tagtatgctc cgtagtgcag caaaaaacca tgaatttgtc
     7381 acctctgttg ttgatccatc tgactatgaa gaagtacttt cagaattacg tcagtatggg
     7441 catacttcac tttctttcag acaaaaacta gcggcaaaag tctttcgtca tactgcggca
     7501 tatgatgcac tgatcgctca atacttaacg gattgggtgg aagaaaaaga accggaaaaa
     7561 ttgacactta cgtatgaacg aaaacaaacg cttcgttacg gtgaaaatag ccatcaagcc
     7621 gctgcctttt atcagtccgc tcttctggaa aaatattcga tcgcagcagc aaaacaactc
     7681 catgggaaag aactttctta taataatatc cgagatgcag atgctgcgct acggatcatg
     7741 aaggaatatg acgagccaac ggtagtcgcc ttgaaacaca tgaatccttg tggtattggg
     7801 acagcatcta cgattttaga agcgtgggaa gcagcgtatg aagcagatcc tgtttcaatc
     7861 tttggtggga tcatcgtttt gaatcgagaa gtagatctgc caacagcaga acagatgcat
     7921 aagctgtttc tagaaatcat cattgcccct tcttatacac aagaagcttt ggaattactg
     7981 caaaaaaaga aaaacatccg attactgaca gtagactttg cgcaggaaga aaatcaaaat
     8041 cgagaagaaa cggtctcagt cttaggtgga cttcttgtac aagatcaaga tctggcaata
     8101 gaaaatgaag aagagtggaa agtcgtgaca aaacgtaagc cgactccagc agaagcaaaa
     8161 gcattagcgt ttgcttggag agctgtcaaa catgtgaaat ctaatgcgat cgtactggca
     8221 aacgaacgtc agacagtagg aatcggagcc gggcagatga atcgagtcgg ttcagtaaaa
     8281 attgcgattg aacaagcgca agagaaaatg gaaggtgcag ttttagctag cgatgcttat
     8341 tttccaatgg atgattcggt tgaatatgca gccaaacatg gtataaaagc aatcattcag
     8401 ccaggaggaa gcatcaaaga ccaagattct attgatatgg cgaataaata cgatctagca
     8461 atggtcttta ctgatgttcg gcattttaga cattagagag gagtcggata aatgaacatt
     8521 ttggtgatcg gtgcaggtgg tcgtgaacat gcaataagta aaaaactttt agccagtccg
     8581 aaggtggata cggtgtattg tgcgccagga aatccgggga tgacaaaaga tggtatcaat
     8641 tgtgtcgcta tcagtgagcg agatcattca gcgttaatcc attttgttaa gaaagaggct
     8701 attgattgga gtatcgttgg tcctgaagtt cctttgttaa atgggatcgt cgatgatttt
     8761 atggcggaag gattgaaaat cttcggtccg acaaaagcag cagctctgat cgaaggttcg
     8821 aaaagttttg ctaaacaatt aatggatact tatcaaatcc cgacagctaa atccagaagt
     8881 tttgaatgtt acgaaaaagc agcagcgtat atcaaagaaa atggtgcgcc aatcgtagtg
     8941 aaagcggacg gcttagcagc tgggaaaggt gtagtcgtgg ctaccacagt gaaggaagcg
     9001 ctggatgcgg cagaacaaat gttgaaccag catcgatttg gggatagtag caaaaaaatc
     9061 gtcgtagaag agtttttggc aggagaagaa ttctcccttt tggcgtttgt ccgtaatacc
     9121 aaggtgtatc cgatggtcat ttcgcaagat cataagcggg catttgataa cgataaaggg
     9181 ccaaatacag gcggaatggg tgcttattca ccagtaccgc aaatttcaaa aaagatgatt
     9241 gaaaaagctg tggaagaagt tttaaaacca gcggctttgg gaatgaaaga actaggacgc
     9301 tctttcacgg gtgttttata tgcgggtttg attgctacga aggaaggacc aaaagtgatc
     9361 gaattcaatg cgcgtttcgg tgatcctgaa acgcaagtcg ttctttctcg gttgaagagt
     9421 gactttgcgc aagtaatcga tgatttatta gagaatcgta cagtagagtt aaagtggcag
     9481 caagaaggat acaatgtagg cgttgtggtt gcagctgccg gttaccccga agaatatgag
     9541 acaggaatgg ttctaccaga ttttctggaa gaagagcttt ctgtttacta tgctggagtt
     9601 tcctcaaata acggcaaact agttggagca ggaggacgtc tttatttagt agaagcatac
     9661 ggtgagacga tcaaagaagc acagaaaaaa atttacaccg cgttaatgga taaaacgaca
     9721 gatggaacat tttatcgtac agatattgga gaaaaagctg ccaatagcga actgatcgtg
     9781 aagtaaatca gctgcttcca tttataaaaa atacaacaaa cataatcgga agcgccgatt
     9841 ttagtgaagc cttttcataa agagtgtggt acacttttgt tgtgtatata aataaggagg
     9901 agttcagatg aaaaaaattc aattaggtac ttctgatcag aaagcagctg cagtgatctt
     9961 aggctgtatg cgtatcaaca atgcggaaga tccagtaaaa gtgattgaga cagcttatga
    10021 aaacggcatt gatttttttg accatgcaga tatttatgga aacggtgaat gtgaagaaat
    10081 ctttgcagat gctctagcaa aaacatcaat caaacgagaa gatcttttta ttcaaacaaa
    10141 atgtggaatc gttccaggtg tgatgtttga tttttctaaa aaacatatca ttgaatctgt
    10201 agaaaaaagt ttgacccgtc tgaaaatgga ttatgtggat gctttacttc ttcatcgtcc
    10261 agatacattg gtagaaccag aggaagtagc tgaagcgttt gatgaattgg aacggtcagg
    10321 aaaagttcgt tattttggcg tcagcaatca aaaaccaatg caaatcgaat tgctgaaaaa
    10381 atatgttcgt caaccattag tagctaatca gctgcaattt ggcttgaaac atactggaat
    10441 gatcgatcaa gggattcatg tcaatatgac agatgaagca agtatcgatc atgacggtag
    10501 tgtacttgat tattcccgat tgcatgatat gacgatccaa gcatggtcac cataccagta
    10561 tggatttttt gaaggtgtat ttatcgggaa cgaaaaattc cctgaattaa accaaacatt
    10621 agaagaacta gcagctaaat atcaaacgac accaactggc ttggcttctg cttggatttt
    10681 acgccatcca gccaatatgc aagtgatcgc cggtacgatg aacgtagggc gaatcaagga
    10741 aatcgctcaa gcttcagaaa ttaatatgtc tcgggaagat tggtacgaat tgtaccgtgc
    10801 tgctggaaac attttacctt aaagattgtc ataagcaggt caaaagggct ataaattaaa
    10861 aagacagaat aacttgaaag ctgttttcag cttcgagata ttctgtcttt ttttattgca
    10921 aatggaaaga gtatcattgt acggatttcg tttattagtt atttttgttt ttaaggaaaa
    10981 gctgtgtccg atattctttt ggagaaatcc cttgtttttt cttaaataaa taactgaaat
    11041 agttagggtc attataacca atttcaaaag caatatctga aatacgatag tctgattgct
    11101 gcaataattt ttttgctaat tcgattcttt gttctgtcag ataatcgatg aatgtttggt
    11161 taaacgattg agaaaaaatc gtactgaaat gagagggact taatgcaact tgttgagcga
    11221 cccaagttaa ggacatatct ggatcggtaa aattttcttt gataaaatcc aatgcatggt
    11281 tcaaaattgg ctgatatttg ttccttgaag gctgtgaggg cagagcagtt aagaaagaaa
    11341 tgaacatata aactgtcgaa cggtaaagcg agtcgtcggc gctgattgct aagcgttgac
    11401 tggtggatgt gcaatgaacc atttcttgcg ggattttctt ttctttcagg cagacatgct
    11461 ggagtagctt agtcaagaca aataaacgat ataatcgtgt tcgagttggt gtgccttgtg
    11521 gtttcgtcaa gtgttcgatg tacttagata cttcgttttt cgataatgaa gacaaatcaa
    11581 gtgagaggtc aaagtaaaag acagaatcaa ctggaagttt cgcttcttga atcaaatcct
    11641 catagctgat aatttgttct ttaagggaac tggcggtttc aagtatcatc tgcgtgatct
    11701 caaaagatgc aggtaattcg ctgattcttt ctgcgacagg accaatggcg acagaaaaat
    11761 tatttttttg tcccttttct aattcatgga ccaacgtttg ggctacctga taactggtct
    11821 cgatcacatt ttcttcaaga gaatcaacta ttaaaaattt gatgaatcga gaggaaacag
    11881 cagagaagat cacttgttga ttctccctaa ataaaaactt caaataatca ctgagatgag
    11941 cgtaatcttc aaagtctgta ctgacttggt ttgctgctaa taatacggtc atttttttcc
    12001 ctgctaaaga gcgattgaaa gattcagcaa gtttcaatgt ttcactgatc gtaagtgagc
    12061 ctttgaacaa accgttcaag aaatgatttt ttttcaattc gaaaacaaga tcagaagaag
    12121 tgtgcgtttc tttttgctgc aaatctaaag cagttactgc tttttctatt gcttgtttca
    12181 attcgctact tttaatcggt ttaagcagat aatcatctgc ctgtacctgg attgctcctt
    12241 tcatataatc gaaatcgtcg aatccactaa taaatatgag acgagtccaa ggaagtagtt
    12301 tccttgcttc ttttgaaaaa gtcaacccgt ccatgaaagg catttggata tcagtcagaa
    12361 tgatgtcggg ctgtaagtcg ataattgaag acagtgccat ctcaccatct ccagcttcgc
    12421 cgacataagt gattggataa taagaaccaa ggacagttat ttgttcccgc aagttattgc
    12481 gaatgaggta ttcgtcttcg atgataaaaa ctttatacat gtttgtttcc tccaattgtt
    12541 ttgggagata gagggatcat cagtgtgaca gttgttcctt ctcggtattt gctgtcaatc
    12601 gtgataggtt tttgatcatt tccgtaataa agaaggagac gcttgtttac gttgtaaagt
    12661 ccgtagccga tactgaaatc tgaatcgatc cctttggcta actctttacg tatttctgcc
    12721 agtttttctg cagggatgcc aatcccgttg tctataactt tgaattggat catctcatca
    12781 attttgcatg cggtaatacg aataaatcct ttgtgacgga cgtacttcgt tccatggtat
    12841 acagcatttt caatcaatgg ctgcaagatc atttttaaaa tagaaacagg aaaaagtgtt
    12901 tctggtatat cgatcttata gtcaagcata tggccgtatc ttatttttaa gatgactaga
    12961 taatcattga tatggctaat ttctttttca agaggaatcc agtcttgtcc tttgcttaaa
    13021 gacgtacgaa aaaagccgga aagtgcatgg gtcatagtca gagcttcttc aaagcagtct
    13081 tgttcgatca aagaaataat ggcatccaaa ctgttataaa taaaatgggg agtaatctga
    13141 gcttgcaata cacgaacttc actttgtgct aaatggtatt gtttcaaagc gttctcccgt
    13201 aaagactggt ttagatcttc cgccatttta ttcatgctag cggtaagtgt ttgtaattcc
    13261 agtgtttccg gcagggttaa acgaaagcca aggttccctg aagaaagttg ttctgacatc
    13321 tcgatcagct gattgatcgg ttcttggacc tttctctcta aaaagcgacg attctttttc
    13381 gcatagaaaa aaatggtact aagaactcca agttccacta ttgttaatag aatgagcgag
    13441 cggacaagtg tcgtattttt tttgcttgct aagtcgatct ccacacgaac aaaatcttgt
    13501 aagatatctg acagcagttg agtgacagaa tctacctgat tgatgatctc ttcattggta
    13561 tccataaaat cttttgccaa gatattattg atcagctcgt tttgataatc ttccagtgta
    13621 tcgatcgtac gtagcgatac atctaacgta ctaatttctt taagtgtatt tgtattattt
    13681 ttgatctctc gaatatcatt cctaagttct tctaggatat tttcctcatt gaactcatct
    13741 acggttactt ggccagaaat caagtcccac atactttcca aaactttttg atcaaccttc
    13801 aaagcaattg catctgcttt ttggatattt gcgatcgtct gttgataaat caaaagctgt
    13861 tgcgaatata acagtatgct cagtagcaaa gggataacag caaatagaag catggctttg
    13921 tttgcttggg caaaaatctg ttttatgctt aaattttttg tcatgatact tctccagtca
    13981 ataatttctg gttgggatgt tagctatatt ctgctgggaa aagacagtct cggaaataaa
    14041 aatatcttta ggtaatgttt ttcctgtttg aatatttcca aaataacgag taacggtatt
    14101 ggctacatac cagccgacat caggtttaca ctcgactaca caattgacga ttcctgagcg
    14161 tagcttctcg atcatatctt cttgtccatc aatagttaca atgatcaaat cttctccagg
    14221 tttgattgat gtttctttta tcgcagctaa agcaccgtga gtcatttcat cactatggct
    14281 gtataataca tcgatttcgg ataaatcatt tgtttctaaa tatcgtttca tctggtcttt
    14341 tcctttcatc cgaataaaat caccgacaat tcggtgaaca atagtcatcg gaggtttatg
    14401 gtcgatggtt ccatcttttt ttagggttgg tttacgattg attgattcca aaaatccttc
    14461 ggagcgtagc ttggtcggtg tggagttctc tgaaccagca agttctagga ttctgatttc
    14521 ctgtttcttt gaatttgtga agtgattgct gacaaataaa cccgcccgat ttccttctgc
    14581 tttgaaactg gggccaatgt gggtgacgaa aagctcggga tcattcgttt gaatatgtct
    14641 gtcgacaagt atgacaggga tccctgctcg ctttgcttct tttaatacag agtcccaccc
    14701 ggattcttgc aaaggagtaa aaatgatcat atccacttta taggcaatga acgtccgtat
    14761 atcttggatt tgccgttcct gattcatgta gccatttcgg tagagaactt ggtaatcttc
    14821 tttttcaagc gcagtgataa ttgattcagt atgctttttc cgccagctac tctccgttcc
    14881 tgattgcgaa aaaccaacga ctagtgggtc aggtgcttcc tttgtattag gtgtacaccc
    14941 catcatttgg taagcgatta caacaagggc taaaaaaaat aaaacacgtt tcactttaaa
    15001 tcactccatc tggatttgtt agtactatct tatcagataa aagggaaaat caaagtcaaa
    15061 aggaatcaat cgaaaaaaat ccaagaaact taaaaaaatc caagacaaaa aaagtacacc
    15121 taaaaaattt aaagtaaatc ccataaaaaa atcaatgtta acgctttctt tattagctat
    15181 actcggtgta tcaattgaaa tgggggataa aaaatgaaaa acaaatttag aaagattgtt
    15241 gggactatcg ctattttgac cgcaagcatt ctattaagtg cgtgtggcaa ttcaggatct
    15301 gcagatgata gtacggggta tgtaggaatc gctatgccga ccaaatcagc agaaagatgg
    15361 atcgctgatg ggaacaacat ggtctcggaa ttagaaaaac tgggttataa aacagatctt
    15421 caatatggag aagataaagt agaaaatcaa gtagcacaaa tagaaaatat gattacaaaa
    15481 ggggtagata cacttgtgat tgcttctatc gatggttcgg ctttaactga tgtgttagct
    15541 aaagcaaaag aagcagatat caaggtgatc gcctatgatc gtttactgat gaattcagaa
    15601 aatgtcgatt attacgcgac ttttgataat tttggagtag gtgtatcgca agcagcttat
    15661 attgaagagc acttaggatt gaaagaagga aaaggaccat tcacgattga attatttggt
    15721 ggctcgcctg acgacaacaa cgcactaatc aattacaacg gagtgatgtc tgttttacaa
    15781 ccatatatgg acaacggtca gttggtcgtg ccatctggac agacgagttt cagtcaaatc
    15841 gctacgcttc gctgggatgg ttctacggcg caagcaagga tggataatct gttaagcgca
    15901 aattatactg accaaacatt ggatgctgtt ttgtcgccat atgatccaat aagtttagga
    15961 atcatttcgt cattaaaagg cgtggggtat gggagtgaaa gtaaaccttt acctgtgatt
    16021 actggacaag atgcgacagt tgctggcgtt aaatcgatca ttgccgggga acaaacacaa
    16081 acgatcttta aagacacacg tatcttagca aaaaatacga ttgaaatgat caaagcaatc
    16141 agtgatggag aagaggttcc ggtcaatgat actgaaactt atgataatgg ggtgaaaact
    16201 gtccctacgt atttagcaaa tacagtatct gtcgataaag ataattatca agcagagctg
    16261 atcgacaccg attattataa agaatccgat ctgaaaaatt aagaggaacc aaacaaggaa
    16321 ctaaagagat ctaagtcaga gagaggataa gaattatggc ggattgtatt ttaaaaatgg
    16381 cagatatcac caaaaaattt tctggaatcc gagcgttaga ttgcgtgaat ctgatgattg
    16441 aacgtgggga aatccatgca ttgtgcggag aaaacggagc gggaaaatca acattgatga
    16501 atgtcttgag cggcttgtat ccatatggct cctatgacgg ggaaatcttt tatgatggaa
    16561 agctgtgtca atttaaagat ttgaaagata gtgaagaaaa aggcatcgtc atcattcatc
    16621 aagaattagc actaagtcct tatctttcaa taaaagagaa tatttttctc ggaaatgaac
    16681 aagcagaaag aggcgtgatt gattgggaac tgacagaaaa gaaaacatat gaactgctga
    16741 ataaagtcgg tttgtctatc gacccgaatc ttctagtatc ccagattggc gtagggcaac
    16801 agcaactagt tgaaatagct aaagcattat caaaatccgt ccgtctgttg attttggatg
    16861 agcctaccgc tgcattgaat gaagaagaaa gcgcgaattt gctgagattg atccgagagt
    16921 tgaaagagca aggtgtaacc tccatcatta tttcacataa gctgaatgaa atcgtggata
    16981 tcgcagatcg aatcaccatt ttacgggatg gacagacgat tgagacacta gaaaatgaaa
    17041 tgatcagcga agagcatatt attcgcggga tggtgggcag agatttagtt gatcgttatc
    17101 ctgaaagaaa accggatatt ggagaagttt attttaaagt agaagactgg acggtctatc
    17161 accctcttga tcacgagcga atcataaacg accacatcaa cttgcatttg aaaaaaggag
    17221 agattgtagg aatagctgga ctgatgggag cgggcagaac tgaattcgct atgagtctat
    17281 ttggacattc ttatggaagc aagatatccg gacgtatatt aaaggaaggg gcagagatat
    17341 cagttaagac cgttccttca gccattgcaa acggactcgc ttatgtctcg gaggaccgaa
    17401 aatctttagg actgaatctg ctgatggata ttcgggaaaa cgtgacgctg agcagtttgg
    17461 ataaaataag tcaaaaagga gtattagata aagaaaaaga agtaactgag gcagaaaatt
    17521 tccggaaaaa gatgcgtata aaaacaaact ccatccatca aattgtcagt agtcttagtg
    17581 gtgggaatca acaaaaagtc gttttatcca agtggctgat gacacaaccc gatattcttt
    17641 ttttggatga gccaacccgt gggatagatg ttggggcaaa atatgagatc tacacgatta
    17701 tcgaagaaat ggcagcaaat ggcaaagctg tctgtcttat ctcttctgaa cttcctgaaa
    17761 tattagggat gtgtgatcga atctatacga tgaacgaagg acgtattaca ggagaagtct
    17821 cgcgagaaga tgcagatcag gaagtcttga tgaaattaat gacgaaagaa gaggtgggtt
    17881 aagttggaac aggcaaaaaa tcttacggat aaaaaagaga aaaaactaaa tttgaaaagt
    17941 agagcagctg atctttttgg tcgctacagc atggtgatca ttttagcggg actacttatt
    18001 gcgtttcaat tgatgacgga tggtattttc tggcgtccat tgaatataac taaccttgtt
    18061 ctgcaaaata gccatatatt agtactagct gcagggatgt tattagttgt gctcttaggt
    18121 catgtggatt tgtcggtagg gtcagtgatg gcgtttgttg gtgcaattgc gggcatgatg
    18181 atggtcaatc gccaaatgaa cccgtggatg acagttatcg catgtcttgc agtaggcggg
    18241 ctgatcggcg catggcaagg tttttgggta gcttatgcag ggataccggc gtttatcgtg
    18301 actttagcag gattgctgat gtttcgtgga ctgacacaag ttgttctagg aggccaatct
    18361 ctagcacctt ttccagctgt tttccaaaaa atgtctacgg gttatttacc agacttaagc
    18421 gaaggaagca gccttcactt cttgacattg attttaggaa tgatcatcac gatcgtgtta
    18481 gttgttggtc aatggcgaac aagaaatcgt cggaaaaatc atttatttga agtggaatct
    18541 ccaacagctt tcttggtaaa ggcggtattt acagcagtcg ttgtactagg aataacttat
    18601 atctttgcct cttatcaagg gtatccgatt attttgatca ttcttggttt gatcgttgca
    18661 gcatatgggt ttttgacaaa taaaacagta gcaggtcgac agatttatgc gacgggcgga
    18721 aatcgaaaag cggctgaact atcagggatc aaaacaaagc gaatcacgtt ctgggtattc
    18781 gtcaacatgg gagtcatggc agctttagct ggattagttt tagctgctcg attgaatgcg
    18841 gcaacacctc aagcaggtac atcgatggaa ctggatgcaa tggcggcagt atattttggt
    18901 ggtgcttcga cttccggcgg cattggaaca atcatgggtg cgattgtcgg tggcttggtc
    18961 atgggtgtat tgaacaatgg catgtcgatt ttaggcgtcg gggtagactg gcagcaggca
    19021 atcaaaggat tgatcttgct tttagccgtt gtgctagact tgtataataa gaaaaaacga
    19081 acaacgtagg agaacgccgg ttattcagaa aaaagactgt gacaagacat ttgtcacagt
    19141 ctttttttcg ctaaaagcga acattcattg tttcgtttgt atagactaaa aataatatat
    19201 gtttttttac cattcgtata aattctcttt ttgctcttga tttttttgct taattgagtt
    19261 aaattacata gggcgctaaa aaaagccttg attaaattaa taatgttcgt gttttgctgg
    19321 atgtgaacat caggaggata atgtaatgaa agtatttgat tacgaagata ttcaattaat
    19381 tcctaataaa tgtatcgtaa atagccgttc tgaatgtgat acaacggtca cattaggtaa
    19441 acatacattc aaaatgccag tagtgcctgc caacatgcag acgattattg atgaaacaat
    19501 cgcagaattc ttagcggaaa atggttattt ctatatcatg catcggtttg atgaagcagc
    19561 tcgaattccg tttatcaaaa aaatgaaaaa gcgtggattg attacctcga tcagtgttgg
    19621 cgtcaagaaa gaggaatatt cctttataga gaagttggca gaagaatcgc taaatcctga
    19681 ttatatcact atcgacatcg cgcatggaca tgctaactca gttattgata tgattcagca
    19741 tattaaaaaa tatttgcctg aaacttttgt gattgccggt aatgttggta caccggaagc
    19801 cgtccgtgaa ttggaaaatg cgggcgcaga tgcaacaaaa gtcgggattg gtccaggaaa
    19861 agtatgtatt acaaaaatca agacaggctt tgggactgga ggctggcaac tggcagctct
    19921 tagatggtgt gcgaaagcag ctcgcaagcc gatcatcgct gacggtggga tcagaacaca
    19981 tggcgatatt gcaaaatctg tccgttttgg tgcgacaatg gtcatgattg gttcattgtt
    20041 tgccggtcac gaggaatctc ctggcgaaac aaaagtcgaa aatggaatcg tgtataaaga
    20101 atactttggt tctgcttcag aattccaaaa aggcgaaaaa cgaaatgttg aaggaaagaa
    20161 aatctggatc caacacaaag gatctttgaa agatacacta gtggaaatgc aacaagacct
    20221 tcaatcatct atttcttatg ctggaggaag agatctggaa gccattcgaa aagttgacta
    20281 tgtcatcgtg aaaaattcga tttttaatgg agacacaatc taaaaaataa ataaaatatt
    20341 aggatgttgt gaaaaagttg tcctgcatta agtaataaga acagccaaac aaaaatagtt
    20401 ctttatatgt tttgtttggc tggaattatt atcgcagata caagcttttg aacatcatta
    20461 ttcagaataa gggactgtga taagattttt gtcacagtcc cttatgtttt gcaaaaaaag
    20521 tttttctttt ttctcgtaaa ttcggtaaaa tcaaaagaaa aaatagacaa tagaaaagag
    20581 aagcttatgt gggaaaaagt agagaaaaaa caaggaccga tttatcgtca aattatggaa
    20641 cagattatga gagaaatcga aaacggaagg atcaatcctg gaagtcaact tgaatcagaa
    20701 cgaaaactag ggcaactttt tggtgtgaat cgttccaccg ttgtccatgc attggaagag
    20761 cttagggagc ttggggtact aacgagcaaa cgtggcagcg gatggtttgt taatcagact
    20821 gaatggggga aattcgctgt tccaagaatc gattggcggc aaatgatctc tcctcgttat
    20881 gagcagacgg actggtatga gcaaaaaata agagaagcaa agcagatcca aaaaacatcg
    20941 tttcttgatt tatacgctag cgagatgcca gatgagcttt tgccgaattt tgagtttcct
    21001 tcttattctc ttgaagaaat cttagcggaa gaaaaagaaa tggatctttt aggctatact
    21061 ccgttacgta acaaaatcaa acggtatttg gaaagtaaat ttgaattggc attttccccc
    21121 aaccagcttc ttgtgactgg tggcggtcag caagcaatct ttttgatttt acaaacgatt
    21181 ctttcagctg gagaggggat agctgtagag tcaccttctt ttttttaccg attacctctt
    21241 ttcaaagctt caggaacgcg tttatttggg ataccgatgg atgaagaagg gatcgactta
    21301 gaaaaactga aagaatcgat ccataaaaat aaattgaaag cagtgcttat caacccgaat
    21361 tttcaaaacc ctacaggaaa agtgatgagt caaaaacgac gagaaaagct agtagaattg
    21421 tgtcgacagt atcggcttcc aattattgaa gacgatgtat ttacggatct ttcatttcta
    21481 aaccaagaag aagtccttcc tatacgaacg attgatccgg ataatgtgtt atacgtcggt
    21541 tcgttatcta gggttttagg aaaaacaacg aaaatcggtt gggtgatagg acctgagact
    21601 tttatctcgc aactagctca agcgcaagaa atgatggagt tttcgatgaa tatccttaca
    21661 caaatcacag tggctaatgt atttaatgaa gcaattgatt tgaaaatgca agatgtaaga
    21721 aaaaagttga aagaaaaaag ccgactgata agcgcttggg gagattcaca atcgtttttc
    21781 aagcttcatt caattttagg tggttactat gcctggatta cttgggatgg gaaaaaacta
    21841 gaccccgaac tagcagaaaa agtagtagat atgggtcttg gaattgcacc aagtctactt
    21901 tttgggcaag atataaatgg aatcagaatc aattacagta ggctggatga aaaccaaata
    21961 ccgttgtttt attcaaagat gaaaattcta gaaaagtggt taacagaata atttcacaaa
    22021 gtaaatagat taaagaatat aacagcttgg atttaaattt caagctgttt cttttttttc
    22081 ggaaaaagaa tacgatataa agaaactaag aaaaaaccat tatttgtacg tccgttcacg
    22141 atgtgtaaat aaaataaatg tttgcaacaa aactgtataa caccaacgtt tttaaataga
    22201 aacagatggt tgtaagtttg attagaaaat agaaaaaatg cagatgaaaa ataaaacgct
    22261 ttccttattg tgattttatt cactaatgct gttaatatgc ggattggcat aatttccttt
    22321 catcgtgaaa atttgtgctt ttagcttgta tttctcataa atccatgata atttattaat
    22381 gaagacggtt aaatagtgat acagttttat tcactgtttc aatcgcagaa tgaagagatt
    22441 ttcttataga tgagagagga atgtgtgaca aatggcaaac aaaaaagcac cgatcgactt
    22501 ccaagcactg ctagatgaag tcaatgcaga tttcccagta tatcaagcac ttgatcaaga
    22561 tggaaacgta gttaacaaag atttagtacc tgatttatcc gatgatgaac tcgttgaatt
    22621 aatgactcat atggtatggt ctcgtgtatt agaccaacgt tcaacagcgc tgaaccgcca
    22681 aggacgccta ggcttttttg caccaactgc tggacaagag gcaagtcagc tggctagtgc
    22741 ttacgcattt gataaagaag atgttcttct tccaggttat cgggatgtac ctcaactagt
    22801 aaaacatggt ttacctttat ctcaagcttt tttatggtct cgaggacatg ctgcaggtaa
    22861 ttcttatccg gaagaattga aagctctacc accacaaatt atcatcggtg cacaatatgt
    22921 acaagcagcc ggtgttgcat taggtttgaa gaaacgaaac aagaaaaatg tcgtcttcac
    22981 ttatactggt gatggcggtt catcacaagg tgatttttac gaagcaatca atttcgctgg
    23041 cgcttaccat gcaaatgccg tattttatat ccaaaacaac gggtatgcga tctctacacc
    23101 tcgtgaaaaa caaactgcag caaaaacact tgcacaaaaa gctgtcgcag ctgggattcc
    23161 tggtattcaa gtcgatggaa tggatccgct tgcagtatat actgtatcta aaatggcacg
    23221 ggaatgggcg atcagtggaa atggaccagt cctaatcgaa acactgactt atcgttatgg
    23281 accacataca ctatctggtg acgatccaac ccgctaccgt acaaaagaca cagatgacga
    23341 ttggcagaaa aaagatccat tgatccgctt ccgtaaatat ttgacggaaa aaggactttg
    23401 gtctgaagaa aaagaagaac aagtcatcga agcaactaaa gaagaaatca aagcagctat
    23461 tgctgaagcc gacaaagtac caaaacaaaa ggtttcagat ttcttgaaaa acatgttcga
    23521 agtatctcca caatcaatca aagaacaaat tgcaatttat gaagcgaagg agtcgaaata
    23581 atcatggcac aaaaaacaat gatccaagca atcacagatg ccttagcact tgaactagaa
    23641 aatgacgaga atgtcgttgt ttttggtgaa gatgttggga aaaacggagg agttttccgg
    23701 gcaactgaag gattgcagga aaagttcgga gaagaccgag tattcgatac tcctttagct
    23761 gaatctggaa tcgcaggatt gagtttcggt cttgcgttag aaggatttcg tccagttcct
    23821 gaaatccaat tctttggttt tattttcgaa gcaatggacg aagtagtagc tcaaatggct
    23881 cgtacaagat atcgaatgag cggcactaga aatctaccca tcacaattcg ttcgccattt
    23941 ggtggcggtg ttcatacacc agaattgcat tcggataatt tagaaggttt gatcgctcag
    24001 tctcctggta ttcgagtagt gattccttcc aacccttatg atgcaaaagg actattgatt
    24061 tcttctatta gaagtaacga tccggttgtt ttcctagaac acatgaaact atatcgctca
    24121 ttccgagaag aagtgccgga tgaagcttac gaagttcctt tggataaagc agcagttact
    24181 cgtgagggaa cagatgtatc tattatcaca tacggagcaa tggtgcgtga agcaatcaaa
    24241 gcagcagaca atcttgcaaa agaaaatata tctgttgaaa tcattgatct tcgtacagtt
    24301 gcgccattag atgtagaaac gatcattcaa tccgtggaaa aaactggtcg cgtagttgtt
    24361 gtccaagaag cacaacgaca agcgggcgtt gccgcgcaag tagtttctga gatctcagaa
    24421 cgcgctatcc tttcattaga agccccaatc ggacgagttt ctgcacctga tacagtcttc
    24481 ccatttggac aagcagaaaa tgtctggttg ccaaatgcaa aagatatcga agacaaagta
    24541 aaagaaatcg cggaatttta agaaaagaag ggacgaaaca aagtcatggc ttatcaattt
    24601 aaattgccgg atatcggtga aggaatcgca gaaggtgaaa tcgtcaaatg gttcgtcaaa
    24661 ccaggagata cgatcaatga agatgataca ttactagaag ttcaaaatga caaatcagta
    24721 gaagaaatcc cgtcacctgt tacaggaacg gttaaaaatg tgattgttcc agaaggtacc
    24781 gtagcaaatg ttggggatgt attagtagaa atcgatgcac caggacacga agacaatgaa
    24841 ggggacagtg gtgtagccgc agaatcccaa actccagcta aaccggctgc tgaacccact
    24901 gttgatacag aatcagctgg atcgtcaagc gaaggagtct tccaatttaa gttaccggat
    24961 atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg
    25021 atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga agaaattccg
    25081 tcacctgtca cagggacagt taaaaacgtg attgttccag aaggcactgt agcaaatgtt
    25141 ggggatgtat tagtagaaat cgatgctcct ggacacaata gtgcaccgtc aactagtgcg
    25201 ccatcagctg aagcaccaaa agaaaaagtg gaaacatcag gttcagcaag tgtcgttgaa
    25261 gctgccgatc caaataaacg tgtattggct atgccatctg ttcgtcaatt tgcccgtgaa
    25321 aaagatgtag atatctcaca agtaactgca actggaaaag gtggcagagt aacaaaagag
    25381 gacatcgaaa acttcttggc aggtggtcca agcagtgcac cagcgaaatc agaagcgcca
    25441 gaagcggcag caccgaaaga agcagctcct gcagcagaaa gcaaaccagc tgcaccagca
    25501 aaaccattca agtccaactt aggagacttg gaagaacgtg tagcaatgac gccaacacgt
    25561 aaagctatcg ctaaagcaat ggtcaacagt aagcatacag ctccacacgt gacattgcat
    25621 gatgaagtag aagtgtctaa actatgggat aatcggaaac gatttaaaga agttgctgct
    25681 gcaaatggta cgaaacttac tttcttgcca tatgtcgtga aagctttaac agcgactgtt
    25741 aagaaatacc cagtattgaa tgcatctatc gacgatgcaa accaagaaat cgtttataaa
    25801 cattactaca atatcggtat tgcaactgat acggatcacg gactatatgt accaaatgtg
    25861 aaagatgcag accgcaaagg aatgtttgct attgcagacg aaatcaatga gaaagcaaaa
    25921 ttagctcatg acggcaaact atctgcagaa gatatgcgca atggaacaat tacgatcagc
    25981 aatatcggat ctgtaggtgg cggttggttt acaccagtca tcaactatcc agaagtagct
    26041 attttaggtg taggaacgat tgcacaacaa ccaatcgtaa atgctgaagg cgaaatcgtt
    26101 gtcggtcgtg tgatgaaact gtcattaagt ttcgatcacc gtatcgtaga tggagcaact
    26161 gcgcaacaag caatgaataa tatcaaacgt cttttagctg atccagagct attaatgatg
    26221 gaaggatgat aaacaaatgg tagttggaga ttttgctgta gaattagata cagttgtaat
    26281 tggtgcggga cctggtggct atgtagctgc catccgcgct gctgaaatgg gacaaaaagt
    26341 agcaatcatt gaaagagagt atattggcgg tgtttgtttg aatgtcggct gtattccttc
    26401 aaaagcactg atagctgcag gacaccatta tcaagagtca ctagattctg aaatgtttgg
    26461 agtaactagt gaaaacgtca aactagattt tgcaaaaaca caagaatgga aagaaaacaa
    26521 agttgtccat acgttgactt ctggtgttgg tttcctatta aaaaaacata aggttgaaac
    26581 aatcgaagga gaagcgttct tcgtagatga tcacacacta cgcgtcattc atccagattc
    26641 agctcaaact tattcgttca ataacgcaat catcgcaaca ggttcccgtc caatcgagat
    26701 tccaggattt aaattcggag gacgtgtgtt agattctact ggcggattag cattgaaaga
    26761 agtacctaag aaatttgtga tcatcggtgg cggtgtgatt ggtgccgaat tgggcggagc
    26821 gtacgcaaac ttaggagcag aagtaacgat tcttgaaggc tcaccacaaa ttttaccaac
    26881 ttacgaaaaa gatctagtaa aattagtaga agatgatttc aagaaaaaag gcgtaacagt
    26941 cgtaacgaat gcaatggcga aagaagcagt cgataacggt gacagcgtga cagtgaaata
    27001 tgcagtggac gggaaagaag aatccgttac tgctgattat gtgatggtca ctgtaggtcg
    27061 tcgtccgaat acagatgata tgggcttaga acaagcgggt gtcgaagtag gagaacgagg
    27121 attgatcacg gtggataaac aaggacgtac gaacgtacct aacatctatg ccatcggtga
    27181 tatcgttcca ggggcggcat tagcacacaa agccagttat gaagcaaaaa tcgctgctga
    27241 agcaatctct ggcaaaaaag tagctgtaga ttataaagca atgccggcag tagccttcac
    27301 tgatcctgaa ttggcttctg taggtatgac gatcaaagaa gcaaaagatg ctggtatcga
    27361 agcaaaagca tataaattcc cattttcagg gaacggtcgg gcattatcgc ttggtaaaac
    27421 agaaggattc atccgtctag ttacgacgat cgaagataac gtgcttattg gcgcgcaaat
    27481 cggtggagtc ggcgcaagtg atatggtctc tgaattagct ttagcaatcg aatcaggaat
    27541 gaatgctgaa gatatcgcct tgacgattca tcctcatcca tctcttggcg aaatcgtcat
    27601 ggatgcttcg gaattagcat taggtttacc aatccatatt tgatttgaat aatacaggcg
    27661 aaacgcaact gaaaaatcag ttgcgttttg ttttttagaa ttatcaataa tattattatg
    27721 taaacttaaa aatatgacaa cttcttggtt tattaccact tcctatgcta cactgaagag
    27781 gaatatgaaa taggagagtt gaagatgcaa gaatatcagt atccacttga tttagattgg
    27841 acaacagacg aaatggtcat tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa
    27901 aaagggataa acaatgaagc ttttttgaag acttatcagc agtttaaaac tgttgtcaaa
    27961 tccattggtg aggaaaaacg attgggacgg gaatttgaaa aagcttccgg ttattcttta
    28021 tatcgtacag tgcagcaagc aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga
    28081 gataaatgga acagatgatt gatcatatca agtcctggct tgaagaagct ggtgagaaaa
    28141 tcaaatcttc tcaacaaaca ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa
    28201 caaatgtcga tcgaaacatc cagaattttc taatcgaaaa aatccattcc tttgatccag
    28261 aggcgaaaat attggcagaa gaagaagggt gtaatcaatt atcggatctc gatggaagag
    28321 tctttatcat cgatcctatt gatggcacct tgaattttgt gattgaaggg gaaaattttt
    28381 gtattatgct cgctgtttat gaatccggcg ttggaaagct tggtttcatt tatgacgtga
    28441 tgagaaatga acttttttgg ggtggaaaag gaataggcgt gtataaaaat gatgagccat
    28501 tgcctagacc agcaaataaa tccttatcgg aaggcttact tggtgtgaat agttatcttt
    28561 tcggtcataa taaatatgga atccgagaaa ttgcggaaag cagcatgggg acacgtatgt
    28621 acggatgcgc tggtcttgaa ctagtggcaa tcttgaaagg aaaccacatt gcttatattt
    28681 caaaccttag cccttgggat tatgcagcgg gaaatgtctt attggaagag tttggtatgg
    28741 taacaagcgg tctttttggt actcctttga catttgaaaa aagggaatac tatttagcag
    28801 ggacttcttg tgcatatgca gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag
    28861 gtagaaatca ttcacaaaaa tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg
    28921 aaaacatttt actatatttc atgaaaaaag tctgctataa ttagcagtcg agtaaaattt
    28981 gccctgtcat aaagaaccaa taaaaaagga gttagttaca ttgaattata gaaacgatat
    29041 ccgtaacgtc gcaattattg cccacgttga ccacggaaaa acaacattag ttgatgaact
    29101 attaaaacaa tcagatacat tagacgcaca tacgcaattg caagaacgtg cgatggactc
    29161 aaatgcgcta gaaaaagaac gtggtatcac gattttagcc aaaaacacag ctgtagacta
    29221 taaaggtatt cgcgtaaaca tcatggatac tccaggacac gcggacttcg gtggcgaagt
    29281 agaacggatc atgaaaatgg ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac
    29341 aatgcctcaa acacgttttg tattgaaaaa agcactggaa caacatatca caccaatcgt
    29401 tgtcgtaaac aagattgaca aaccttctgc tcgtcctgag catgtcgtag atgaagttct
    29461 tgaactattc atcgaattag gagcagatga tgatcagtta gactttcctg tgatttatgc
    29521 atctgcacta aatggtacgt caagcttgtc agatgatcca gcagatcaag aaccaaccat
    29581 ggcacctatt tttgatacaa ttattgaaaa aatccctgct cctgtcgata acagcgacga
    29641 accattacaa ttccaagttt cattacttga ctacaacgat tatgtaggtc gtatcggaat
    29701 cggacgtgta tttagaggaa caatcaaagt aggagaccaa gtagcattaa ttaaattaga
    29761 cggaacagtt aagaaattcc gtgtaacgaa attatttggt ttctttggac taaaacgtct
    29821 agagattcaa gaagcaaaag ctggagactt gattgctgtc tcaggtatgg aagatatctt
    29881 tgtcggtgaa acagttactc ctgtggatca tcaagatgcg ttacctatcc ttcacattga
    29941 cgaaccaaca ttgcaaatga ctttcttagt taataattca ccatttgccg gtcgagaagg
    30001 taaattcgta accgctcgta agattgaaga acgtttgatg gcagaacttc aaacagatgt
    30061 atccttacga gttgaaccaa caaactctcc agatgcatgg acagtttctg gtcgtggaga
    30121 attgcatttg tctatcttga ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc
    30181 tcgtccagaa gttattgaaa aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt
    30241 gcaaatcgat acaccagaag aatatatggg tagtgttatc gagtccttga gcttacgtaa
    30301 aggtgaaatg caagatatgg tccatacagg aaacggacaa atccgtttga cattcttaac
    30361 acctgcccgc ggtttgattg gttattcaac tgaattcctt tcaatgacac gtggttacgg
    30421 aatcatgaac catacttttg accaatatct gccaatgctt cctggtcaaa ttggcggacg
    30481 tcaccaaggg gcgcttgttt cgatcgatac aggaaaagca acgacttatt ctatcatgag
    30541 tatcgaagaa cgcggtacag tcttcgtaga accaggtaca gaagtatatg aaggaatgat
    30601 tatcggggaa aacagccgtg ataatgattt gactgtcaac atcacgaaag caaaacaaat
    30661 gacaaacgta cggtctgcta ctaaagacca aacatctgtc atcaaaaaac caaaacaatt
    30721 gacgttggaa gaatctttgg aattcttgaa tgatgatgaa tattgcgaag taacaccaga
    30781 aagcattcgc ttgagaaaac aaattttaga gaaaaatgct cgtgaaaaag catctaaaaa
    30841 gaaaaaataa tctgtaaaag gatgaagaga gaaaccagct tgttataaaa aatagctggt
    30901 ttcttttttt gtccaactct tttctgtgga gaagtcatct ttgtcaatgg acataaattc
    30961 agaaaatacg ccgaaagacg tttaaaagac ttttaaaaaa cttatttcta gagagttttt
    31021 gtttcataat aagataatgt ttcacgaaag gagatcaact tgtccttcga agaaaatcga
    31081 agtaaaatgg caagagaaag gagcgggaag atgaacgact tattaaaaca atactttggt
    31141 tatgatgagt ttagacctgg tcaaaaagaa attatccaga aagtcattga tcaggaaaat
    31201 gtattaggaa tcatgcctac cggaagtggg aaatcaatct gttaccagct tccagcttta
    31261 ctattagatg ggcttactgt ggttgtgtcc cctttaatct ctttgatgaa ggatcaggtg
    31321 gacgcggcga atcaattagg tatacccgcc acttttatca atagctcgtt agatggttac
    31381 gaaacagcta gacgcttcca agaaatcgat agacagcagt accgtttact ttatatcgct
    31441 cctgaacgtt ttatcatgcc ggattttatc caagcaatga agcgatggaa cgtccgtatg
    31501 atcgctattg acgaggcaca ttgtatctct cagtgggggc atgatttccg cccaagttat
    31561 ttacagatgg cgaaccagct agatcaactg ccaaatcgtc ctgtaattgt tgctttgaca
    31621 gctacagcga ctgtgcaagt agctgctgac ataaaaagac ttttaaaaat tccagaaaac
    31681 aaccatattc agactggttt tgaacgagaa aatctacgat ttcaagtaat taaagatcaa
    31741 aaaaaagaac aatatctaat cgaatattta aagatcaaca aaaatcaatc tggtatcatt
    31801 tatgcagcaa cgagaaaaga agtagaccgt ctctatcatt tgcttaaaaa attcgatttt
    31861 tccgtgggta gatatcatgg tggtctaaat gagaatgagc gaacagaaat gcaagaagcc
    31921 tttttatacg atcgcttgca gctgattgta gcgacaaatg catttggtat gggtataaat
    31981 aaaagcaatg tccgttttgt tatccattat caaattcctg gctctctaga agcatactat
    32041 caggaagctg gtcgggcggg aagagatggt ctttccagtg aagccatcct gcttttctct
    32101 cctcaagata tccaagtcca aaagtttttt atccagcagt cccaaagaga agaagggcaa
    32161 aagcaaaaag aatatgaaaa attaaaagcg atgacagaat acgtgtatat tgagtcctgt
    32221 cttcagcaat acatcctaaa ctattttggc gagacaagca gtccgtgcaa ccgttgtggt
    32281 aactgtttag atgacagaga aatagtagaa gtaacgacag aagcacagat ggttctttct
    32341 tgcttaaaac gtatgggtga gaactatggt aaacaaatgc tgatgaaagt cttagcagga
    32401 tcgaaagaac aaaagctgca agctttaggt tttggacatt tatctaccta tggactgatg
    32461 aaaaaccaat cgcaaaaaga aacgatgcag ttaattgaat atttgatatc gaatggctat
    32521 cttctgacca tcaatggtga gtatcctgtt ttgaaagtaa cagaaagagg gatacaagta
    32581 ttgaaaggac aagaatctgt ttatcgaaag gaacccaaga aagtccagca gctatcggat
    32641 gaagaaaccg atacactatt tgaagtgctg cgggaactgc gaacggatct agcatcagaa
    32701 gcaggcgttc ccccgtatgt cgtattttca gattccacat tgaaagaaat gagccgaata
    32761 cgtccatcaa gcagattgga gatgcttcag ataaaaggag ttggccaaag caagcttgat
    32821 aaatatggcg aagccttttt atctcggata aaaaatgcgg actctaacgt tctggataag
    32881 tagaaagaac gaatacgtaa aaatgaataa aaacaactgg ttaaatctgt tttttttgct
    32941 ccttatgaaa atcaaatatg ttataattaa ggaattagaa tgggaggtaa gcacagatgg
    33001 attctttgtc gaaaaaactt gcgatccaag tattaaatga tgaagcagaa cggatcaaac
    33061 gattaatccg aaatcaaaac aacagtctct gtatctcaca atgcaaagcc ttcgaagaag
    33121 tggtagatac acagatgtac ggcttttccc ggcaagtcgg ttttgctgtc cgtataggag
    33181 taatcggaaa gaacgagggc cagctgatgt taagtgagct agaacgagaa ttgaatcagt
    33241 tatatagtga ggtttaccaa gaaatttatg ataaaaaaga agttggcaag gaggaataga
    33301 cttgcctaag aaagtgaaaa aaaggcatct attagactat agcatcttga ttccctattt
    33361 aattttatgt gtattgggat tgatcatggt atatagttct acgtcgtatc tattattaga
    33421 aaacggacaa aatccgtccg catcagtcat taaccagagt attttttggg tgcttagctt
    33481 gattgctatc gcactacttt ataaaatgaa aacagatgtt ttgaaaaacc agcgtttgat
    33541 catggcagcc atcgcagttt tgacgatttt gctgctgatt gttgtgtttt ttggtaagga
    33601 gatcaatggg gcgaaaggtt ggctgcaaat tgcaggattc tcgatccaac ctgccgaata
    33661 cctaaaaatc atttctatct ggtacctgtc tctgacttta tctaaaagac aaaacagtgt
    33721 acaaaaagat tttcttggga ctgtgaaacg tccacttgca atggttatcg gacttactgc
    33781 attagtcgct attcttcccg attttggtaa tgcggcagtt atctttttga ttattttggt
    33841 tctgctttta gccagcggag tcaattatgt gtatactttg attgtaggag taggaggctt
    33901 ttgtctaagt acttttacga tctggctgat caatatcaca aacggaaaga tcttacccgg
    33961 acgtttacag tatatctata atcgatttgc tatttaccaa aatcctttct cagacgaatt
    34021 aaataaaggg catcaactag tcaacggcta ctatgccatg ttcaacggag gactatttgg
    34081 caggggatta ggtaacagta tccagaaaaa aggattttta caagaagcac aaaccgattt
    34141 tatttatgca atcgtagtgg aagaactggg agtgattatg gggattttga tattagcgtt
    34201 gctattcttt atgattgtgc gcattgtcct tgtaggtatc cgttcaaaag atccgttcaa
    34261 ttcgcttttg tgcattggga tcggcgcaat gtttttgatc caagtatttg tcaatcttgg
    34321 aggaattact ggcgttattc ctttgacagg gatcacgttc ccatttttaa gtcaaggagg
    34381 ctccagtttg ctgatgcttt cgatttgtgt cggctttgtc ctcaacatca gtgcggatga
    34441 aaaacgcaag agcttaggtt tataataaaa aaagatgtag gagtggttcg atgaaaaaag
    34501 ttttagttgc taacaggggt gaaatcgctg tacgtgtatt tcgtgcttgt acagaattag
    34561 gaattaaaac tgttggaatc tatgcagaag aagatgaata ttccgtacac cgtttcaaag
    34621 cggatgaagc atatttagta ggacaaggaa aaaaaccgat tgatgcgtat ctagatattg
    34681 aagggatcat ttctatcgcg aaagaatgcg gagctgacgc tattcatccg ggttacggtc
    34741 ttttgtcgga aaacttgaat tttgcgcaac ggtgcgaaga agaaggcatc atttttgttg
    34801 gacccaaact gcatcattta gatatttttg gagataaaat caaagcaaaa gctgcagcta
    34861 tcgaagcggg gattgcttcg attccaggca cagatggacc aatcgcttct atagacgatg
    34921 ccttggagtt tgcgaaacaa tacggttatc cgattatgat caaggcagct ctaggcggcg
    34981 gcggacgcgg catgcgtgta gctcacgatg aaaagagtgc aagagagggc tacgaacgtg
    35041 cgaaaagcga agcaaaagca gcctttggta gtgatgaagt gtatgttgaa aaatacatcg
    35101 ctaatcctaa gcatatcgaa gtacagattc tcggggatac gcatggaaac gtcatccatc
    35161 tttttgaaag agattgttct gttcagcgcc gtcaccaaaa agtagtagaa gtagcgcctt
    35221 gtgtctctat gaatgaacag caacgtcaaa aaatctgcca ggcagctgtc caattgatga
    35281 aatatgtcgg gtatgtcaac gcaggtacag tagaatttct tgttgaagga gatgattttt
    35341 actttatcga agtcaaccct cgcgtccaag tggaacatac gatcacagaa atgattacag
    35401 atattgatat cgtaacaacg caactgttga ttgctcaagg tttggatctg cataaagaaa
    35461 tcggattgcc tcagcaagaa ggaattaaat taaatggttc agccattcaa tgccgaatca
    35521 ctacagaaga cccgttgaat aactttttac cagacacagg gaaaatcgat acgtatcgtt
    35581 cacctggtgg ttttggcgta cgtctggatg tcggaaatgc ttacgcgggc tatgtagtga
    35641 caccgtattt tgattcattg ctagtaaaag tatgtacgca tggtgctacc tttgaaacag
    35701 ccattcagaa aatggaacgt tgcttgaaag aatttagaat ccgtggagtg aaaacaaaca
    35761 ttccatttat gctcaatgtc atcacacatc ctgaattcca atcaggaaat gcaaaaacaa
    35821 ccttcattga tagtacagca acattatttg aatttcctcg actacgagac cgcggaaaca
    35881 aaacgatgaa atatatcggt gaaattactg tgaatggttt tccaggtatc gaaagtggcg
    35941 aaaaaccatt ttatgaagaa ccacgtatgc ctaaagatct aatcacaaga actgattacg
    36001 taacggctaa gaatgtatta gatgcgaagg gtgccgatgc tttggtcgaa tggatcaaag
    36061 gacaggaaaa tttgttgttg acggatacga catttagaga cgcccatcaa agtctgctag
    36121 caactcgtgt tcggacaaag gatttcaagc agattgcacg tttgactgga gaaggtcttc
    36181 ctgaactctt ttcaagtgag atgtgggggg gagcaacatt tgatgtggct taccgctttt
    36241 tgaatgaaga tccttggcaa cggttgagaa aaattcgttc tttaatgcct aatacgttgc
    36301 tgcaaatgct tttcagaggt tcaaatgccg ttgggtattc taattatcct gacaatgtat
    36361 tggtggaatt tgtcaaagaa gcagcagcgc aaggaatcga tgtatttcga atctttgata
    36421 gtttgaactg gactccacaa atggaaaaaa gtatccaagc tgtacgggat actggtaaaa
    36481 tcgcagaggc agctatttgc tatactggcg atatcaacga tccaagtcgg gcaaaataca
    36541 atgttcagta ttataaagac atggcaaagg aactagaaca actgggtgcg catatcattg
    36601 caatcaaaga tatggctggg ctgcttaaac cgcaggcagc ttatcgtttg attagtgaat
    36661 tgaaagaaac gacagatctg ccaatccatc tgcatactca tgataccagc gggaatggaa
    36721 tcatcactta ttctgctgct tcaaaagcag gagtagacat cgttgacgtg gcaatgagtg
    36781 caatgagtgg gaatactagt cagcctagca tgagcagctt gtattatgca ctggtcaatg
    36841 gtccacggct gccagaaatc acgattgaaa atgcacagaa attaaaccat tattgggaag
    36901 atgtgcgcat gtattacaaa ccgtttgaaa acggcttgaa tgctcctgaa acagaagtat
    36961 atatgcatga aatgccagga ggacagtatt cgaatctaca gcaacaggca aaagctgtcg
    37021 gattaggtca tcgctgggat gaaatcaaac aaatgtatca tactgtcaac ttgatgtttg
    37081 gcgatatcgt taaagtaact ccatcatcta aagtagttgg agatatggca ctatttatgg
    37141 tccaaaatga tcttacagaa gaagatattt atgaaaaagg cgaaacacta agtttccctg
    37201 aatctgttgt gactttcttc caaggagaac tagggcaacc cgttggcgga tttcctaaaa
    37261 aattgcaaaa gattattctt aaaggcagac ctgcattgaa tgaacgtcca ggtcttttgg
    37321 ctgaatcagt cgacttcaac gaagtaaaaa aagaattggc agaaaaaatt ggctatgaac
    37381 caaaacaaga cgaagtatta agctatttga tgtatccgca agtctttttg gactatcaaa
    37441 aagcatataa tcaatttggc gatgttacat tactggacac accaacattt ttccaaggta
    37501 tacgtttggg tgaaacaatc aatgtacaaa tcgaacgcgg aaaaacgctt atcattcgtt
    37561 tagatgaaat cggagaaccc gatatcgaag ggaatcgcgt actgttcttc aatttgaatg
    37621 gacaaagacg tgaaatcact atcaaagata attcgatcat cagtacggtc caaacccgat
    37681 tgaaagctga accgacaaac cgagaacaaa ttggagcaac gatgtctggt tctgttttgg
    37741 atgtattagt gaaaaaaggc gataacgtta aaaaaggtga tacgttgatg attactgaag
    37801 cgatgaaaat ggaaacggcc atcgaagcac gttttgacgg agaagtcgca catgtatatg
    37861 tgtcttcagg agacactatt tcgtctggtg accttttgat tgaagtgact gagaaataat
    37921 aagcgaaagg tggggaaaca tgaaaagact gggtttgttt tggctgattc tgctattagt
    37981 tattgtggca ttctacatgg aaccagtctt gtttccgcca aaacttgaag tgaaggaaaa
    38041 ctctgatcaa caaatatcaa accaaatgac aacgtggaaa taccaggagc ttaaagcaac
    38101 tggtttttct acttatatcg gaaaaccgat cgatcagctg gaaaaggatt ttggccaagc
    38161 ctatgatcga ttaactagcg gatttggttt tgaaacaaga tattatgaaa ataaagctag
    38221 tcatctcaca tttgaagcga acatccaaga cagcagagtc acaactgtta aagtactgca
    38281 aacagggcct gctgatatcg cgccgtttta tttagggatg acgatgcaag atcttacgaa
    38341 aatcactact atctatacta atttcacttt tgagtaccat gatacagaag ttggaatcga
    38401 actaatggaa gaggatatga attatcgtcc gctaattgct tttgataacg acacttttgc
    38461 tattttattt tttgatcaaa caacaaacgg cctattttca gttgtgtatt tagataaaga
    38521 tagtcttttg aaactattgc catatcaagt gtttggtgaa ggccttccac attatcaagc
    38581 agataaatcg gctgattggg aatcaatcaa tcgcgttaaa gaacgaaaaa gtacgacttt
    38641 attaaatatg cttagaagag aagacgaact tcccatatac aatcaaacac ttgcctttca
    38701 aaaccacagt cagctgctac tacatgattt tttgacaagg cctgaggaaa tattgtcaga
    38761 ggaacgtatg gctgattggc aaaaatcgct aggcagtgct caaacgacag tgaaattctc
    38821 actgacgaat gatgaattgg agaagttaat cagtaaacaa aaattagaaa atactagtgg
    38881 gctatttatt catccggcga ttgacgctac tttttcgttt ttatattggt acagcgatcc
    38941 ttacaaccat gaccgtttca tggacccggg aacggattct gtaggcattg ctttttctaa
    39001 agaaaatatg ttagtcttat tgcaagaaga aaacggagaa agtagtgata gcagtgatcg
    39061 ttaacgaaga gctgtttcaa ctggaggatc aatgcctcca agtcgctgaa atgataaaaa
    39121 agagtaaaac gatggcttct tatcttgaac ataaacagtc aatggaagaa tctgcaaaag
    39181 tttgccagtt gaaagaagcg ttcttgtcta agaaagaagc atttgaaaat gtagcggcat
    39241 atggtgagta tgcacctgat tatcgtgaaa aaaaacgagc tgttcgaaaa gcaaaaagac
    39301 agcttgatat ggatgagcat gttgcagctt tccgatatag tgaaacacag ctgcaaagtc
    39361 tgttggatca aattgtccaa gtacttgccg aagaagtttc taatgaaatc aaagtagatg
    39421 caggtagccc gttttttgaa acgaaaaaac actttggatg cggaggtaat tgtcatgcaa
    39481 ttagacaata gtgaatttga aataacaaaa cgacgcgggc tgatcgtatg ggtctacagc
    39541 ttaaaacaat taaagaatct gaaaaagttc ggcttgttgc attatgtctc aagaagaatg
    39601 aaatatgtcg tgctttactt aaatgaagag acattggaag aaacagaaga acgaatccgt
    39661 aaattacatt ttgttcgaaa cgtagaacgt tcttaccgac cggatatcga aatgaatttt
    39721 gccgaaaaaa taggaaacaa aacagtatta aaagacgaag gtttcgaaat tgaagagcta
    39781 aatacagaaa tccgattagc tgaacatctt taaaaatctc ttatgagcag tttttaaata
    39841 aaaaataact tggataacga tcgatttttg atcattgtcc aagtattttt ttggtatact
    39901 agtaccagta ccatttctaa ggagtgagta gatgcggata atatcaggaa attatggcgg
    39961 gcgccgttta aaaagtttag caggggcgaa caccagacca actacagata aagtaaaaga
    40021 atccatattc aatatgattg gaccatattt tgatggagag acagtattgg atctgtttgc
    40081 aggaagcggt ggtttagcta tcgaagcagt atctagaggc tgcagtcatg cggtatgcgt
    40141 agacaaaaat taccaagcgc taaaaatcat caaagaaaac atcgaaatta cgaaagaacc
    40201 agagaaattt accacaataa aacttgatgc ggacaaagca atcgaacggc tagcagcgga
    40261 gaaacaacaa ttcgactact tgtttcttga tccgccatat gcaaaacaaa aaataatcga
    40321 tcaaatccat aaaatggaag aactgggact tttttctaaa accgcagtta tcgtatgcga
    40381 aacggataaa acagtcgaat tgccagaaac tgtggctgga ttccatcaaa taagaaaaca
    40441 gatttatggc ataacggccg taactatcta tagaaaagag ggaaatcaat gaaacgtgca
    40501 ttattcccag gaagttttga tccttttaca aaaggtcact tagatacggt ggaacgtgcg
    40561 gctaaattat tcgatgaggt agtaattggt gtttttatca atacttcaaa gaaaagtttg
    40621 tttcccccag aagaaagaat gacattgatc acaaaagctg tttctcatct tcctaatgta
    40681 aaagtcatgc atcaagaaaa tcaattgacg gtagaaacag caaaagagat tggtgcagat
    40741 gcgttgatca gaggcatacg aagtatcaaa gactttgaat atgaacgaga gattgctcag
    40801 atgaatcatc atctatatga tgaattggaa actgtcttct tattagcaaa accagaatat
    40861 agtcacgtga gttccagtat tttaaaagaa gtactgcact ttggcggaga tgtgagcagc
    40921 tacttaccgc cagtgatcaa cgaagcttta gctggaaagc gtgaatcaaa tgaaagctaa
    40981 acaatggttg aaaacagtac tgattatcat atttggaatg ttgatcattg gatttgtagt
    41041 agttcccttg ccgtattatg tagaatcacc gggagccacg atcgatctga aagaactaat
    41101 tacggtcaat gataaggaag ataaaggttc tgggtctttc tctcttacct ctgttggtat
    41161 caggcaagct acaggtttct tagctttaaa agctaaaatg accccttttg aagaattgat
    41221 cagtgaagaa gaattgactg gtggggcgac aaatgaagaa tataatcaaa tgcaacagta
    41281 ttatatggat tcttcccaaa atgcagcaat cgaacaagcg ttaaaacttg cagacaagcc
    41341 ttataaaatg gaattcaaag gcgtatatgt catgagtgta gagaaaacat cgaatttctt
    41401 tggcaagctt tcagttggag atacagtaac aaaagtagac gggaaaagct ttcaaagtac
    41461 agaagcattt atggattatg taaaaagcca aaaagttgga caaacaatcg aaatcagtta
    41521 tttgcataat ggtgaagaaa aaacagcttc tggtgatctg atcgagttac caacagataa
    41581 aaaggcagga ataggtatca cgcttactga ccataccgag atcgaatcag atacagatgt
    41641 tcgtattaat tctggctcta tcggtgggcc atcagcggga ttgatgttca ctttagaaat
    41701 ctatgaacaa gtcactggta aaaacttgcg acatggtaag caaattgcag gaactggaac
    41761 gatcaacagt gaaggagaag ttggccgaat cggaggaatc gataaaaaag tggcaagtgc
    41821 ggataaagct ggcgtagaga ttttctttgc accagatgat acgatttcag acgatgttaa
    41881 gaaagaatat cctgaaataa aaaccaacta cgaagaagca aaagctgccg ctaaaaaatt
    41941 gaattcctca atgaaaatcg taccagtaaa aacagtacaa gaagcgcttg actatctaga
    42001 gaaaatgaaa tgaaactaaa taattaaaga ggggttgtga cacgagttct gtcacaaccc
    42061 ctctttttcg tcaaaaatct tcacgataaa aataaaaaca aaagaaaaaa ttgacgtaat
    42121 tagatgttga agggacgatt ttgccagagc tcactgtttc ttcaatttta aatgaaagga
    42181 gtaatgaatg atttttactg gtcattcttt gaaaaaagaa caatgataga aaaaatcagc
    42241 aagaaaatac tagcttcacc caaattgatc atttggatgt ctatgatagt tcttatcttt
    42301 tgtatcgcaa gtggtttttt attatcaaat ttgctatttt atagagaaac agaagagact
    42361 gctgcaaaag aaatactttc ttcgtttagt tccagtggag aagatccatc agctgaagga
    42421 aacgatgtag aagaggatgc tcgtccaaag gtcatgtaca ccgatatcaa aggaagtgtg
    42481 aaggagcctg gtatctattc cttttcttca gaagaacggg tctatgatgt actaaagcgt
    42541 gcaggtggat tattagaaga agcggatagc gatcgaatca acttttctgc gaagatagaa
    42601 gatcagcaag tcctatatat ccctgctgta ggtgaagagc tgccagaaca tcttgatcag
    42661 ttggcatcct cggaaggaaa acagcctaca gcagattccg agcctcccaa aataaatatc
    42721 aatacagcca ctccatcaga acttcagcag attcctggga tcggatcagt caaagcgcaa
    42781 gaaatcattc gatttagaga agaaaacgga tccttccaga aagtggaaga cctgcaggag
    42841 atttctggta tcggtgaaaa gacagttgaa aaactaaaaa acttcgttac aattaaatag
    42901 aatgattcaa aatcgactgg cacgagcaat aaatggacgc taatggatac taagaataat
    42961 ccaattctaa gagcttggca ggacgaggag gtagtatttt tggataggaa atagcttgat
    43021 tgttcgcaaa gaaacattaa aaaaaccaat gctgaatgtc tatctccaaa ataaaatatc
    43081 tgggattcat attatgaata cggctgtcag cggcaataac agtcaagctc tacgagaacg
    43141 ctttgccaaa gatgtactta gctatacagc agataaagta tttattctca ttgggacgaa
    43201 tgatctagca gagcataaac aactttctaa agaaacgtac caaaaaatct gcagtggatg
    43261 atagttcagc tgaaaaaaga ctatagttct gatcatgctt tctttatcac gccaccagca
    43321 gtcgatgaga caaaacaaaa atatcggaat aacaagttga tcaaagaata tatctctgtt
    43381 ctcacagaag tagcaaaacg ccatgattgt gaagtatcga tttttatacg gaatttcttg
    43441 aacaaatgga ttcgaagaat ttttataatg ggctaatgga tgacggactt catttcggca
    43501 gtgaagggta ggatcttttg tcagacagga tgattcgtta tatttaaaga atattttgtt
    43561 taagcaatga gtacttttta atcaacttga aaaaacggag gaatataaaa catgttagaa
    43621 aaatcttcaa aagcatgcga tacgaataaa gaaaaagtta ctgataagtc acataatgaa
    43681 cgcattcctt gggaccagta ctttatggct caggcagtac tgttatcttt acgtagtacg
    43741 tgtacacgtt tagaagtagg agcgacactc gttcgagaaa aaaggatcat tgctggcggc
    43801 tataatggag cagtcagtgg agatgtccac tgcatagatg aagggtgtta cgtcgtggat
    43861 ggacactgct tgcgaacgat ccatgcagag atgaatgcac tcctacagtg tgcaaaactt
    43921 ggtatcccga cggaaggttc agaaatctat gtcacacatt ttccatgttt ggcctgtaca
    43981 aaggctttgt tgcaagctgg tgtgaaaggg atcaattatc tccatgacta tcgaaatgat
    44041 ccttatgccc aagcgttgat cgaacaatta ggtgtatctg tacaccaagt tgctttagac
    44101 tcacagcatt tttcaaaact tcaaaatgaa ttaacaggaa aacatgtcta gcgttttaga
    44161 aaggcatgtt cgaaatcggt tgatttttcc ggcaattacg tcgatgatta tctgtttgtt
    44221 gtattacact gacaattggg gttatcgttt gcttcttgcc ggaatttttt tatggttttc
    44281 gctaaaagaa ggacgtttta tcgcgtactt gtgtattttg tgtagcataa cggtatccat
    44341 ttcttatagg ctacaagagt attcattttt agaggaaaaa acaacatctg cgcaaattac
    44401 tgtatttaca gatacgattc gtgcaaatgg taattttttg acaatggaag gaaggttatc
    44461 agagaataaa caaaagctcc agctttctta tcaagtaaac acagaagcag aattgaaaac
    44521 atggcttaac ttccgaggaa agggaataga attgactgct gaaggcgagt tcatctctcc
    44581 agcaaaaaag cggaatcttt ataattttga ccaagaagct tacttcagga accatagaat
    44641 ttctggacgg ttccaaattc aaaagattga acagatcacc atgcaaaatc actggcgaaa
    44701 ctggcttcag agaaaaagag gatcttttat ctcctttgtc caatctgaat ttccgcaaaa
    44761 aacaagtgta tatattaatt ctttattatg gggctataaa gatgcaggat tcagagaacc
    44821 agaagaaatt tttagggaga gtggtttgct gcatctgttc agtctatcgg gtctacacat
    44881 ccaattttat ttagggtggc tgtattattt atttcgacgt atcggatggc cactacacgt
    44941 cagtattatt ccgttgagtc tactaactat ctgttatgtt tctattgcag gaagttctat
    45001 ttctgttttg cgtgctagtt tgctgttttt attgcgattg ctagtcaaat tgctgaataa
    45061 gcattattcc accatggact tatttgcagt tgtcttttgg cttcttttac tatttgatcc
    45121 tcgatttctt tttcatgcgg gtggacagct tagttttttc atgagttttt tgtttatcct
    45181 gatttctttt gatggagatt ggttgaaaaa aacggcctct cacgtcctgt ttactatgat
    45241 cacgatgccg ttgattgtat ggcattttta tgagtggcct attttaggta gtctttttac
    45301 attactgatt gcaccggtat tcaagtttct ttttcttcct ggtgtgttat ttctcacact
    45361 cttcaatcgc tggctgccgg aaactttact tttgctgata gaagagggat taatcttatt
    45421 tgagcgaata atcgattttt cttcagggtc gtctctcatt ataggacgtt tgccccttgt
    45481 gctatctggg atgtatattt tcggattact gatttgtatg gataggttga aagaacaacc
    45541 tgtgaaacac ttgttttatg ttggtctttt ttcttttatt tttttgactt tgccattttt
    45601 acgcttttct tcaacgatcg cttttatcga tgtcggtcaa ggagacagta tctttttgca
    45661 aagtcctttt cgaaaagaaa cgattttgat cgatactgga ggtcagttac actttgaaag
    45721 agaaaagtgg gcgcaaggta tgatacgtcc tcctgctgaa agaaatatcg ttccatatct
    45781 aaaaggtcag gggataagtg taatcgataa actcatcttg acacacgacg atacggatca
    45841 tgtgggagaa ttacccacgt tatctcagca ttttactatc aaaaaaattt atatcggttg
    45901 gggcgctgga cgaaatgagc cattacaaag gcatttatct gaagctcaaa aaaatggtac
    45961 tgagatcatt gaaatcaaac atggggacag gatcagcggt tattatgatc tttatgtatt
    46021 gactcctttt gaaaagggag agggaagaaa tgaagattcg attggtctat ggatggaata
    46081 taacaatagg cgctttttat ttttaggaga tttgaatcaa gcaatggaaa aacaattgct
    46141 tttgatttat cctaatctga aagccgatgt agtcaaatta ggtcatcacg gtagccgtac
    46201 gtcaagtaac acagattttc tttcccatat tgaagcaaaa catgggatta tatcctgcgg
    46261 cgtaaataat cgttatggtc acccgcacag cgaagtattg gagacactag aagaaaatca
    46321 gataaaaaca tggcgaacgg atcaacaagg aatgatcacc tatcgctggc atccagtttt
    46381 tcatcctcat gggctagtgg aaacaatgat agactagtaa ttctcatctg ctcatggtag
    46441 gataattttt gaaagtggtg agtaatttga gtatacaagc agagttacaa aaaatacgta
    46501 aagaaaaact tgcatcttgt tacttgattt taggaacaga aaagtttctc caagatcaag
    46561 ttcggacaga aattgtcaaa aaaatccaat tagagggcga agatgactta aattttttaa
    46621 gttttgacat ggaaaatgca agtttagatg aggtagtagc tgaagcagag acgttgccgt
    46681 ttttcggaga tcaacggtta gtattcgtag agaatcctta ttttttaaca ggtgaaaaag
    46741 taaacaatgg aatcgaacaa aatactgatt tattgacggc ttatttgaaa gaaccattag
    46801 aatcgactgt attagtattc tttgcgccat atgaaaagtt ggatgaacgg aaaaaagtaa
    46861 cgaaacaatt gaaaaaaaca gcaattatca tcaatgtcca acagttgaat gaaaaagaag
    46921 tacgtcaata tctgatgaat accttagaga attcctcaat caaaatggaa cgaagtgcaa
    46981 ttgatttatt cttgaggttg accgacttag atttatccaa aatgatgaga gagttacaga
    47041 aattggtcct ttatggacaa aatcaacagc aaatcacggt aagagaagta gaagaattag
    47101 ttcctaaaac gttagaacat aatatttttg acatgaccca atatatttta aaagggaaga
    47161 cggaacaagc tttgcgttta tacgaagatt tagttatgca aggggaagaa acaatcaaaa
    47221 ttaatgcgat tttgctttct caattacgtt tatttttaca gacaaaattt cttgtaaaga
    47281 ttggttatca gcaggcaaat attgctgaaa cattgaaaat ccatccatat cgtgtaaaac
    47341 ttgccatgca ggaagtaaaa aaattcgatg aacatttgct ggtacagttg tttgatcaat
    47401 tagttgagat ggattatcaa atcaaaacgg gacaaatcga aaaagaatta agttttcaac
    47461 tattcgtttt gcgcacaggg cagttgttaa gaaatcgata agaagaaaga cgagggcaaa
    47521 gcatgcggga aggtcgtttg tttttgtcct ttttttgaca aataaaaaaa tcggccgaag
    47581 ccgattcttt tatttagcga tttttttgct taaacgagat ttgtcgcggt tagctttgtt
    47641 tttgtggatt agaccttttg attcagccat atcaatagct ttaacagctt ctttatataa
    47701 cgcatctacg ttgtcagcac ctgaagctac agcatcttca aattttttga ttgcagtacg
    47761 catagcgctt gtttgagatg aatttttcac attagcgttt tcgcttgtac gaacacgttt
    47821 gattgcagat tcaatgtttg gcattagttt cacctccgat aacttaagtt cttacgcgtg
    47881 ttaccgcata attcaacatt cattattata cctaaaggca gcgtgctttg caatatgaac
    47941 atgacaagtt tttttcctta acagtctttt attaggattt tttttcagta aaaacggata
    48001 gtacaagctg aaaaaaatca aaaagacgct attgtgggag gcaatagcgt caaaccaaac
    48061 tagattttta tttatctatt taaacaaagt ttgaatggga atgtttggta ctaagttatt
    48121 atataacaaa gagaaccgtt tgcaaagttt tttttgaaaa gggatacgat tccctgttgt
    48181 tctttgtttt tctattaaga aactgcagtt attttataac cagtatcctt tgatcagtaa
    48241 aggctattct ggtgtaccga ttattttaat gtattcatcg atatttttta tcaacatttt
    48301 ttttatgacc aactggttcc gacggttcca tttgccactt acttccagca catatttatt
    48361 taggggcaaa cgtaagatat attttgccaa atagcgatct ttaagcaggc cattgatcac
    48421 acggtcctca agctgcaagt tgatcccagt cagcatcatt ggctgttctt gtaaaacgta
    48481 cgtatcaatt aaaagacctt caaaagtttc cacaagcgtc ttcctttctt ttcaaagtaa
    48541 cctgaaaaca ggcactaatt cttccatttt gatattcgtt gaagtggatg cgatcattct
    48601 ttcttttgtc cactgtaatc tagtataaca aaaaagagca gaagagccaa aaagaaaaaa
    48661 gagcatcgaa ttctatagga agtctcattg gcacggccta aattattaca aaacattgat
    48721 taaaaaaagc ccctgcatcg caggagcatc ccctatatcg ttttaatagg tcaatcaaaa
    48781 agcagtatca aacacatttt gtatcaaatc aggaaataat agataagcta agcccagaat
    48841 caaaagtacc gctagaagaa accagccgat tgttcctatg aaatacttta atttttcgaa
    48901 cattgtatca cctcgttgtc agtatatcac ggatcagtaa attgggggtg ttaagaatct
    48961 cctaaaatag acaaaatttg ttctattttc ctaaagcaaa tacttgtaag atacagtaag
    49021 tgataccaga agcaatgacc ggtccggcgg cgattcctct taagaagaca acacctaaaa
    49081 tcgtgccgaa aacaagtgca acagtgattt gtggatcaga agcaatcaat cctacaccct
    49141 tggaagataa gacagccact agcccaccgc aaaaaacagc tacccagcca ataggggatt
    49201 taaaggcttc taatagatca cgtagaccga tttctcctgt tgcgataggg attagaatag
    49261 ccgctgtgat gatcgtcacc ccaatattga ttcctttgcc ttgaagccaa gggaaaaagg
    49321 aacgggaggc tggtatgagc ttcataatca aaacaaaagc tgccgctatc atcagacttt
    49381 gatttttagc taaaattgct acaagtaaaa taattcctaa aaaaagccaa ctttccatga
    49441 aatatattcc tcactttctt cgaaacaatc ataacatgtt tttagaaagt tacaaaagta
    49501 ggaattcatc cttcaagacc tattcataag ctgttttttt cataaaataa acaaaatcat
    49561 aaaaaaacac tttgcttatt tgacagaaga cagccgcgct ttgtatagtg gacaagactt
    49621 acattttttt gaagaatcag aaagatcagg taaaggtctt agtaaacgca agccattcag
    49681 aataaccaat attgtgcttc cttcgtgtcc tacaactcca agaggtagat ttaaaaattg
    49741 caggaagtta gaagcaatca acaagagaat aacaagaatc gaaaaaataa tattttgttt
    49801 gatgatccgc cgcattttta aagatagccg atgactgtag acaaatttat ttaagtcgct
    49861 tttcattaag acgatgtctg cgatatccat agcggcatct gtcccttgcc ccatagcgac
    49921 acctaaagaa gcggctgcta aagcaggcgc atcattgact ccatcaccaa ccatcgcatt
    49981 tacttggtaa agttcattct cttttttgac tagttgtgtt ttttcttccg gagtacagct
    50041 tgcatagtaa tgttcaatcc ctacttgttc agcaatggcc tttgctgtgc cagaatggtc
    50101 gccggttatc atacttgtat ggatgttttg ggatttaaag taggaaatta cttgttgggt
    50161 atttgctttt ggaatatcta gcagacctaa aacagcaatt aactgctgat ctttagaaag
    50221 ataaatcacg gttttgcctt gattttccaa acgttcgatc atttccgcaa tctctttgct
    50281 gattatcatc gtctcaggat catatgcgtg cttgccaact ttccattgcg cacctaaata
    50341 ggtagtttgt aatccaaaac cagtaatttc ttctattggt aagtattgga tttcttcagg
    50401 tattgtgcaa tcgaaatatt gcaaaatcgc atgagcaagt ggatgcgtcg ttttttgttc
    50461 catagcaaca agaagttgtt gtgccaaatc cttttcatca aagaaaaagc tgtcagttac
    50521 aactggtttt cctttagtga gcgttcctgt tttatcaaag gcaatcgctt ttaattcaga
    50581 aagcttttct aaatggatac cgcctttgat taaaatccca ttttttgcag aatttgaaag
    50641 agcagctaga gaagctggtg tagcagatgc gacaagcgca caaggagaag ccacaactaa
    50701 taataccatt cctcgataaa agctttcatc ccaactccaa cctaggaaaa agtgggggag
    50761 aaggatcatt agaggaacag caagcaaaat caattttaca taattatttt cgattcgttc
    50821 gataaatgaa gctgtttgag ttggcgtatt ctgcgcttct tctactaatt ggataatctt
    50881 agcaaagaca gtttcggaac tcgtttttgt tactcgaatc gtgatgggat ttccttgatt
    50941 cagcgttcct gcatacatct catcgcctaa atatttttct acaggcacag attcaccgct
    51001 taatgcagac tcatcgatcg ttgcctgtgt gctttctaaa aagccatcta tcggaacagc
    51061 tgctccttta gcgacaaata ccaagttatc cacctttagt tgagaaacat ctacttcttg
    51121 catttgtcca ttttctttta ataattgggc tttttgtggt tgaagattca tcaaagcagt
    51181 aatttctttt tgacttttat tagttgtgta ttcctctaat gcaccgctta aacaaaagat
    51241 gaatgtcaac attgctcctt caaagtaatt accgatcaaa caggcaccaa tagcagctaa
    51301 agccatcaat aaatccacat tcagtgtccg atcattccac aattctttca agccttccga
    51361 tgtttgttta tatccaccga tgataatggc actgatataa aaaagtgggg tgatggtaag
    51421 agagaatcgg tcgaagataa aaccgaagat cataaataaa aggcagccga ttgttgctgc
    51481 aaaagctttt ttctgattag tcatcgttat tagacctcct tgtcaaaaca aggatagcat
    51541 aataaaaatg taattgataa taataacagc taaatgagaa cgataatggt tatcatttgg
    51601 tgat
//
LOCUS       DAPCMT010000005        50419 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPCMT010000005 DAPCMT010000000
VERSION     DAPCMT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 50419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.005"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..379
                     /locus_tag="RD743_000213"
     CDS             <1..379
                     /locus_tag="RD743_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDU0562075.1"
                     /translation="EKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIE
                     IVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKK
                     ADYVTVSNDKDGVSQAFERILTI"
     gene            660..1880
                     /locus_tag="RD743_000214"
     CDS             660..1880
                     /locus_tag="RD743_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDU0562076.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1877..4597
                     /locus_tag="RD743_000215"
     CDS             1877..4597
                     /locus_tag="RD743_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0562077.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4581..5525
                     /locus_tag="RD743_000216"
     CDS             4581..5525
                     /locus_tag="RD743_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDU0562078.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5594..6604)
                     /locus_tag="RD743_000217"
     CDS             complement(5594..6604)
                     /locus_tag="RD743_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDU0562079.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6784..7128)
                     /locus_tag="RD743_000218"
     CDS             complement(6784..7128)
                     /locus_tag="RD743_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDU0562080.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7121..7555)
                     /locus_tag="RD743_000219"
     CDS             complement(7121..7555)
                     /locus_tag="RD743_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDU0562081.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7693..8430
                     /locus_tag="RD743_000220"
     CDS             7693..8430
                     /locus_tag="RD743_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562082.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8423..9634
                     /locus_tag="RD743_000221"
     CDS             8423..9634
                     /locus_tag="RD743_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562083.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9838..10620
                     /locus_tag="RD743_000222"
     CDS             9838..10620
                     /locus_tag="RD743_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDU0562084.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10690..11352
                     /gene="trmB"
                     /locus_tag="RD743_000223"
     CDS             10690..11352
                     /gene="trmB"
                     /locus_tag="RD743_000223"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDU0562085.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11450..13204)
                     /gene="ade"
                     /locus_tag="RD743_000224"
     CDS             complement(11450..13204)
                     /gene="ade"
                     /locus_tag="RD743_000224"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDU0562086.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13336..14265
                     /gene="tadA"
                     /locus_tag="RD743_000225"
     CDS             13336..14265
                     /gene="tadA"
                     /locus_tag="RD743_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDU0562087.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14222..15271
                     /locus_tag="RD743_000226"
     CDS             14222..15271
                     /locus_tag="RD743_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDU0562088.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15268..15546
                     /locus_tag="RD743_000227"
     CDS             15268..15546
                     /locus_tag="RD743_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDU0562089.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15521..15991
                     /locus_tag="RD743_000228"
     CDS             15521..15991
                     /locus_tag="RD743_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDU0562090.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16008..16256
                     /locus_tag="RD743_000229"
     CDS             16008..16256
                     /locus_tag="RD743_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562091.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNM"
     gene            16327..16698
                     /locus_tag="RD743_000230"
     CDS             16327..16698
                     /locus_tag="RD743_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDU0562092.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16670..17029
                     /locus_tag="RD743_000231"
     CDS             16670..17029
                     /locus_tag="RD743_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562093.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17201..18208
                     /locus_tag="RD743_000232"
     CDS             17201..18208
                     /locus_tag="RD743_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDU0562094.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18233..19417
                     /locus_tag="RD743_000233"
     CDS             18233..19417
                     /locus_tag="RD743_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDU0562095.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19854..21437)
                     /locus_tag="RD743_000234"
     CDS             complement(19854..21437)
                     /locus_tag="RD743_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDU0562096.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22015..22158)
                     /locus_tag="RD743_000235"
     CDS             complement(22015..22158)
                     /locus_tag="RD743_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDU0562097.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22380..22859)
                     /locus_tag="RD743_000236"
     CDS             complement(22380..22859)
                     /locus_tag="RD743_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDU0562098.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22932..23216)
                     /locus_tag="RD743_000237"
     CDS             complement(22932..23216)
                     /locus_tag="RD743_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562099.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23294..23488)
                     /gene="ssrS"
                     /locus_tag="RD743_000238"
     ncRNA           complement(23294..23488)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RD743_000238"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23560..24846)
                     /locus_tag="RD743_000239"
     CDS             complement(23560..24846)
                     /locus_tag="RD743_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDU0562100.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24984..25655
                     /locus_tag="RD743_000240"
     CDS             24984..25655
                     /locus_tag="RD743_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDU0562101.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25621..26109
                     /locus_tag="RD743_000241"
     CDS             25621..26109
                     /locus_tag="RD743_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDU0562102.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26122..27069
                     /gene="prmA"
                     /locus_tag="RD743_000242"
     CDS             26122..27069
                     /gene="prmA"
                     /locus_tag="RD743_000242"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDU0562103.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27070..27834
                     /locus_tag="RD743_000243"
     CDS             27070..27834
                     /locus_tag="RD743_000243"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDU0562104.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28067..30280
                     /locus_tag="RD743_000244"
     CDS             28067..30280
                     /locus_tag="RD743_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDU0562105.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30291..30737
                     /locus_tag="RD743_000245"
     CDS             30291..30737
                     /locus_tag="RD743_000245"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDU0562106.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30908..32167
                     /locus_tag="RD743_000246"
     CDS             30908..32167
                     /locus_tag="RD743_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDU0562107.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32256..33203)
                     /locus_tag="RD743_000247"
     CDS             complement(32256..33203)
                     /locus_tag="RD743_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDU0562108.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33597..34889
                     /locus_tag="RD743_000248"
     CDS             33597..34889
                     /locus_tag="RD743_000248"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDU0562109.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34904..36673
                     /gene="aspS"
                     /locus_tag="RD743_000249"
     CDS             34904..36673
                     /gene="aspS"
                     /locus_tag="RD743_000249"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDU0562110.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36782..37657
                     /locus_tag="RD743_000250"
     CDS             36782..37657
                     /locus_tag="RD743_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDU0562111.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37923..39806
                     /locus_tag="RD743_000251"
     CDS             37923..39806
                     /locus_tag="RD743_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDU0562112.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39881..40165)
                     /locus_tag="RD743_000252"
     CDS             complement(39881..40165)
                     /locus_tag="RD743_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDU0562113.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40155..40889)
                     /locus_tag="RD743_000253"
     CDS             complement(40155..40889)
                     /locus_tag="RD743_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDU0562114.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41018..41641
                     /locus_tag="RD743_000254"
     CDS             41018..41641
                     /locus_tag="RD743_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDU0562115.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41833..42954
                     /locus_tag="RD743_000255"
     CDS             41833..42954
                     /locus_tag="RD743_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDU0562116.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42961..46089
                     /locus_tag="RD743_000256"
     CDS             42961..46089
                     /locus_tag="RD743_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDU0562117.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46108..49302
                     /locus_tag="RD743_000257"
     CDS             46108..49302
                     /locus_tag="RD743_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDU0562118.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49448..50155)
                     /locus_tag="RD743_000258"
     CDS             complement(49448..50155)
                     /locus_tag="RD743_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDU0562119.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16461 a         8854 c        10507 g        14597 t
ORIGIN      
        1 agaaaaggat gctatcagca tttacaaatt ttttattttt attcctaatc gtcaattgaa
       61 agctaatata tttaaccatt tgaaaaagat gctaaatgtg tctgttactg aatcggcacc
      121 tgtaaatatt gaaatcgtac ctcaaaatgt atctaaaggt gtggtgtttg atcgtttgga
      181 atcaatctac aatttaaaga aacctttgag aatcgcgatc ggagatagtt taaatgactt
      241 atctatgttt gaaagtgcag atattagttt tgcaatggga aatagtcatc aagaaatcat
      301 gaaaaaagct gattatgtca ctgtttcaaa tgataaagac ggagtatctc aagcatttga
      361 aagaattctt accatataaa ctaattaagg tagtatagaa catagaattc taaatacgga
      421 aaaatggagc ataaattcca gtacaaatag tacttcttca gtgtcaaacg aagcgaatat
      481 aacatagata tttaggattg tgacaaaaat catgtcacaa tctttttttc gaataaactt
      541 attttttgaa gtcggccatt tatcgtaatt tctttgcata atacgaacaa attttcggtt
      601 attgatgaaa aaaaacattg aaaatggtat gataaaagaa aaaaaggagg aaatttttaa
      661 tggtgaaatt tatccatgcg gcagatttac atatggatcg gtcatttgaa ggactgacaa
      721 ctcttgataa aactgtacag gaaaaactat tgaagacgaa tctgaccgtt ttatcaaata
      781 tcattgaaca ggcaataata aataacgttg attttgtttt acttgcagga gataattttc
      841 atcaaaatcg tccttcatta aaaatacaga aacatttttc agaacagatg aaacggttag
      901 aaaagaacca tatacctgta tttataatat tcggaaatca tgatttctat caaaaagagc
      961 ggtattggtt ctcctttcct aaaaatgtcc atttatttac atccgaaacg gtagagacaa
     1021 aaaaaataac gataaaaagt ggagaaactg tctctctcag tggatttagt tatcgtcaac
     1081 cgtggattca aaaagacaaa gtgatggaat ttccttctag agaactcaca gattaccata
     1141 ttgggttgta ccacggggaa cctggcgtct ctcaaaaagg aaactatgct cctttccaac
     1201 cctcaaagat gcaagaaaag ggatacgatt attgggcttt agggcatatc catgtgccaa
     1261 cggtcttaaa tgaaaagcaa acaattgttt atccaggtgc gcctcaaggt catactaaaa
     1321 aggaacaagc ttctacatct attctactag ttgaattaag taaaggaagt tgccaaatac
     1381 atccgattaa agtagcagag gtctattgga agacaaagga aatatcattg aggaaagcaa
     1441 gaacgacaaa agaggtcatt gttcacattc gtcagcagct ttcaaaagta gaggatggat
     1501 ttagtttaat tgagataaaa ttaaaagatt atgaccatct caatacagat gttttggaac
     1561 ggatacaatc aggagagcta ttagactatt tgttagagga attctcagat aggataaatg
     1621 atttctttat ttggcgtatt tctctcatag agaatacttt ttatacgaag accccacttg
     1681 ctgcttctgc taaattgatg gaacagttat ttcaatacta tcagacacct caagatttcc
     1741 aacaaatttt aaatgaagta tatagtcatc aagaagcggc tagaatattg agcgaactgc
     1801 cggaatatca agaagaaaca cttgaaaaag ccaagcagct gcttaatcaa gattttttat
     1861 ttgaggagga tcaggaatga agattttaag gatcgagatt gctgcttttg gaaaatggcg
     1921 tcaaaaaagt tttgactttt attcaggcaa tcagctgatc tatgggggaa acgaagcagg
     1981 aaaatcaacg atttatcaat ttattcaagc aatattattt ggtttccctt caaaaggccg
     2041 taaaaaaaag gattatacac caaaagacgg ctcagcctat ggcggcaaaa tatggcttaa
     2101 acatcctgta tatggtgaat ttgctgtcga gcgctacaag cagcaaaatc gcggaaaatc
     2161 gaaggtgtgg ctgggggatc aagtgggaag tgatgagtta ctagaaaaac tgatcgcacc
     2221 tttgacgaaa gagttattcc agcaggtatt tactttccag caagagcagt tgatggaact
     2281 agatcatttg agagaaaaag agctacatga tgcgctgatt tctttaggca tcacgggcag
     2341 ttctcaagta ttccaaaagc ggcaagaata ttatcagaaa gcacaacaat tattcaagaa
     2401 aaaaggccaa aaattactaa tcaatcaaaa attaaatgaa tggaaagagc tgcaagaaaa
     2461 aattcatcaa aaacaagaac aggaagctta ttttggcaag ttgatccaac aagaacagga
     2521 atacgagtcg caacagcagc agttattggc acaattaaaa gaggcgaaca aacaattttc
     2581 attagccaga cagcaagaac ttaatttttc tttatatgaa gaatggcaat ctctcaaaaa
     2641 aaacagcact acaggtcttc ctgatgagca acttctttta gatctggagt ctttctccaa
     2701 acggtatcag caaacaaatg agcgtttgga agaattagaa gcaacactgg aaaagcatag
     2761 cgggatggat cagcagtcag ccagatatta tttttattta gagcaggagc aggaactaaa
     2821 agatctccag caacttcaat ttacagcgga acaattaaac gaagaatggc aaagattaca
     2881 aattgagact agagaaacaa attcagaatt gaagattttg gaacaaaggt ggcattggca
     2941 gaaaaatccg ttaccggaag aatttttata tgaagaagaa tggaagcatc ttctggctga
     3001 agaaagaagg attgctgatc tagaaagcca gatttctgtg cgtctccaaa tagctaaaga
     3061 acagcaagag atgattgaag cagaagtgaa tgacttggaa gagacctatc cagcattatt
     3121 acaaaatcag tcagagatag caccagtctc ttcatctaaa ccgtatatgc tgatggctgg
     3181 cggagcaata atcattgcag gtatatttgt accaatgccc ttgaaagtga tccttttact
     3241 aattggtgca ggcatgattg gctatttctt ctacaaaagg caaccaaaaa ttaaaactaa
     3301 aaatgatttc tctgagataa aaggaatgtg gcaagaaaaa ctaggacaac ttgatctagc
     3361 cttagctcaa ttagctgaag cagaagaaaa acaaaagaga actggagaag aaaaacagat
     3421 tcttcagcaa gaagtatatc gttttgcgga tcatcggcac ttaggaaaga tggatacgct
     3481 caaactcatg aaagaatatg gacaggaagt catagattat cagaaattat atggcgtatt
     3541 tgagcggaaa cataaaagag aacaggaaat acaagaaaaa ctacgacaaa tcgaccaaaa
     3601 atttgagttt gttaaagaat ggttaccgat acaagataag acaatttcag aaaaaatggt
     3661 attgctttcc cgttttatac aagagatgga acagatgaaa tttgccagaa gctatcagca
     3721 aaatacttta ttgaagcaag aaatcaatca attgaaaaag aaacaaaaag tatggataca
     3781 agaatacagc gaacaattga ctcaagcagg cttatcttat ccttcggaag tacctgtctt
     3841 tttacaaaaa gccaaacaag ccgaacagca gatgaaaaga tacaatgaat tatcggaaat
     3901 gcttactcca ctatttcctg aagagctgac aaaagagaaa ttatctgtaa gactgcgcca
     3961 actgaaagaa aagcaatccc aactccagca agaagaaaaa gaagcaagtg aaaatcggca
     4021 acgactccaa cttcaagtag aagagttgca aagagacggc acattggacg aactttatca
     4081 acaagctagc cggcaaaaag caaaattaca ggaattactg gaggaatggg gagcacttga
     4141 aatcgccggg acattcttag gtgatctttc aacagagatg agtgaaagac aattacccca
     4201 gttgttaaga gcagcaggtg attatttagg tattttaaca aaaggaaatt atcagaagat
     4261 attactgaaa aatgatacac tgactttaac agacggatat acagaatttc ccatctatga
     4321 gttatctaca ggtacgaaag atcagctgat tatggcgatg agatttgctt acctctcatt
     4381 agaagctaac cgttcgatat gcccgatcat cattgatgat ggctggcttc attatgatca
     4441 tcagcgaaag tatcaattag ctgaactttt agctaaattt ggagaaaagt atcaaataat
     4501 ttgtttttct tctgataaag aaatggtaag ctattaccat gagatgaacc aaatcgtaaa
     4561 acaattagaa ggagcaaata atgaaaaaaa ttcgtgattt agtaatcgat gagcagtttg
     4621 aacaatttgt cctgatcaaa agtgcagatg taagaatcgc taaaaacgga aaaaaattta
     4681 ttgcttttac tttccaagat acttcaggta ctatcgatgg taaatactgg gatgcttcgg
     4741 aggaggaaat caataaattt acagccggca cagtcgtttt tctaaacggt aaaagagagg
     4801 tttatcaagg aaaccctcaa gtaaagattc ttcatatgcg cttaacaagg gtagatgagc
     4861 caggagagcc tagtttatac atggaaagag cccctttgaa gaaagaagaa atggaagaag
     4921 aaatcaatca attgttgttt gagatcacca atgcgcattg gaatcgtatt gttcgctatc
     4981 tacttaataa atatcaaaaa caattttttg agtttccggc agcaaaaaga aaccatcatg
     5041 catttgccag tggattagct tatcatactt tgacaatgct tcacttaggg aaatcaatcg
     5101 ccgatgaata tcctgagctg aatcgagcat tgttgtatgc tggtatcatt ttgcatgatt
     5161 taggaaaggt gattgaactt tccggtccta tgtcgacaga atatacgctt gcaggaaatt
     5221 tgatcggtca tctggtattg attgacgaag aaataactaa agcctgtctt gcgttgaaaa
     5281 tcagcgaagc tgacgaagat gttcttgtat tacgtcatat ggttttagca caccatggct
     5341 tattagaata tggctcacct gtacgtccaa gaataatgga agcagaaata ttacatcaaa
     5401 tcgacaatat cgatgcttcg atgcagatga tgttgacttc gattcgtcaa acagaaccag
     5461 gaaaatacac cgatcggatt ttcggtatgg acaaccgaag tttctatgta ccaaaagatt
     5521 tatagcaaaa atttattttt ttcattcaaa acaaaaaaag cgagacataa gtcttcgctt
     5581 tttttgtttt cttttatttt gatgaatcag ttgtttcttc agttgaagat tcttttgttg
     5641 aatcagttga ttttgtgtca gaactctttg ttgaagctgt agtttcactt gagtctttcg
     5701 tacttgatga agttgttgtg aatgcactca acacattttc aaaggcatca tctttgattt
     5761 ttacatttgc atcttttaat tcttcaccga ttactttagt tgtgaatgca ttgtcactta
     5821 gtttagtttc agtagcgata tcttttagtt gatctttgta tttatccatg tcattgcctt
     5881 tattttggtt tttcaccatt ttgacaacgt agtattctgt cgcataagaa gtaggatttg
     5941 ttgtagtaat aacatcggag atctcaccgt ctttcaattt gaaagctgct tcttttactt
     6001 ctgcaggaat agttgttgtc gttgagtcga atttaacttt accgccatct tcttttgtct
     6061 ctgtatcagt tgatttgtct ttggctaatt tagagaaatc atctccatca tccgctgatt
     6121 ttttcacatc ttttgcttca tcttcacttg ataatttgat gatttgtgct tctacttctg
     6181 gatggaaaga tttccaagca gttttcaaat cgtcatctgt aatatctacg tgagctttca
     6241 agccagcttc aaaagctaaa ttgttacgaa tatattcttt ataagtatct ttcgtatatc
     6301 cagcagcttc taattgtgat tcaaaagtat cacctaatga tttcgcttgt ttgtcgtatt
     6361 ctgcatccac ttgcttatcg gttactttat cgccatattt gttattgaat actttataaa
     6421 tgatcatccg ttgtactaag gattggttcg atgattcaag ttttgcttca tcatagaaat
     6481 cagatacagt gatcgttccg cctttcatcg ttgcgatatc tttattagta tcgcctgaac
     6541 aagctgctag agttaaaaca gccaacgcac tagtagctgc caaaataatt gatttttttt
     6601 tcattaaaat ttgcactcct gtaattagtt attattttta tatattaact ataccatatc
     6661 ccatttcctt taaaaacgtt gtcgtggtag tttcaaacaa aattcaaaat ttcatcacat
     6721 tcctaggaaa tacgcaaact tttctcactt atatagttaa atctgttgag aaaataatca
     6781 ttattatatt tgttcaggat actctggcag atcagctttt agtttttcta cttgttcatt
     6841 gatttgtgtg atacgtggtt cggcttggaa tttaaaatct tcgatatctt ttttgattgc
     6901 ttcttggaaa gcaggaatca cagattcagc tgtttctttt gtttcgatca atgcgctttt
     6961 aaaatgattc aggctgttat ttaaatcatt tgtcagagtt gttgcttctt ccaattcttc
     7021 gatcagtttc tttcttgttt cattcccgct cctgggcgcc aataacagac ctccgacagt
     7081 gccagcagca gctccaaata acagtccttt gaaaaagttt ttaatcatcg caggacacct
     7141 gctttacgat tgtttcttga atagctttta gttcttccga tccataacta tcttgatgat
     7201 tgacaaagtg aatagaaaaa tcatcctctt tgctgtagcg agggactaaa tggatatgtg
     7261 aatgaaaaac agattgatag gctagttcac ggttgttatt caagatatta agacctttca
     7321 tttctggaaa agctttttcc aatgcttgag caatcttagg cacacgcgaa aaaacctctc
     7381 ctgcaagttc gggatcatat tcaaaaatat ctgctacatg acgtttaggc acaactaatg
     7441 tgtgtccttt cgttgcttga gagatgtcta aaaaagcata gaccttgtcg tcttcatata
     7501 ttttatagct tgggatttct tgattgatga ttttgcaaaa aatacagttt tccatataaa
     7561 acatcctttc tataaagaat aaatacttca gcttaacttt accataagtt aaagctattg
     7621 taaggaaaaa aacaaaatac cgtcattttc ttcgtggtat aataaataaa aataaatggc
     7681 ggaggaaaaa gaatgagttt aattatcgat catgtcacag gggggtacgg acatctccct
     7741 gtgttaaaag atatcagttt cgatgtacag cctggagaga tagtagggtt gattggactg
     7801 aacggagcag ggaaaagtac gaccatcaag cacattatcg gtctgctgga agcttcaaaa
     7861 ggaacgatag aaatcaatca tctatcctta aagaaaaata cggaagaata tcgcaaacaa
     7921 attgggtata ttccagagac tccttcttta tatgaagaat tgacattgag agaacatatc
     7981 gaagtcactg cgatggccta cgatatacct atggatatag caatgaaaag agcagaaaag
     8041 ttgttggaga cttttcggtt atctaatcgt cttgactggt tcccagctaa tttttcaaag
     8101 ggaatgaaac agaaagtgat gatcttatgc gcctttttga tcgagccttc tttatatatc
     8161 atcgacgaac catttttagg cttggatcca ctagctatca atgcattgct tgaattgatg
     8221 gtagaaatga aaaaagaagg agccggtatt ttgatgtcta cgcatatttt agctactgca
     8281 gaaaaatatt gtgatcgttt tgtggtctta cataacggag agatacgagc agacggttct
     8341 cttgagatgt tgagaaaaga atttcagctg ccggattctt ctttggacga aatctatatt
     8401 gctttaacaa aggagcaggc acatgagtag tttttttcaa aaacgtttag caagacatca
     8461 aaagaaaatg atgaaatata tgcgttatgt attgaacgat cattttgttt tagtttgtct
     8521 tttcttgatt ggcggtgtgg gattatatta ttctaattgg ttgaaaaccc tgcagccacc
     8581 gtttgcaatt ggagggctca taatctctgt attttggatg ctctgtttat tcgtgggtaa
     8641 gatggctact tttgcagagg cagcagatat tgttttttta cttcctaaag aaaaagaaat
     8701 gcaaacctat ttggaacgag cttttcgcta ttcttgcatt tttccgttga tctttttgat
     8761 attggcatgc ggattggcca tgccattagt ggtggttagt acggagcaat ccttttcgat
     8821 gtttttcgtc tatctgatca tattatggag tttgaaattc agtcatctgc aaatcaagag
     8881 agcagaattg tttcgactag atcaacaagt tattcgttat tggaaattag gctggttttt
     8941 ttcttctctg atctttttgt ttctatcatt ttattggcta atatgggcag gaatagcagg
     9001 tgcattagta caggcaagtc tatattactg gtttttgtgg aagcaacaag aaaaaggatt
     9061 ggattgggag aaaatggtgc atgtagaaga acataggttg catcagatct atcaatttat
     9121 caatttattc acagatgttc ctgaaattac ggcaaagatc aaaagaagac gctatttgga
     9181 tcctatattg actcatatta aaagagaatc aaaaaacact tatctttact tgtttgcaag
     9241 aagattggca agaggttctg attttagtgg tctttatttc cgattagtaa ttataggtgg
     9301 gttattgatt gccggagtag tcgactttta ttttagtctg attatcggtt tcttattttt
     9361 atatttgatc ggtttccaac tgttaccttt agcgaaccag ttccgctata tggttctcgt
     9421 tcagctttat ccaatccata agaatcagaa gaagcaggcg attcagtatc tactcacatg
     9481 ggtattaatt atcgcgtcgg ttatttttgg tttgattgct gcagtcgtat tagatgggca
     9541 gcagcgatgg gttccgctag cggcttattt gattgttact atgctctttg ttaaactata
     9601 tgtaccaact cgtttgaaga agatgtcaga ctgaaaacaa agctgtgtag ggtaaaaatg
     9661 agaatgaaaa atagagcgtt gacagtaaga atgctgttcg ttctattttt tttgagaaaa
     9721 gctggttttt tgaatggcaa ttaaatgaaa aagaacgtat atgtgcttga cgtgtagtgt
     9781 tcaggcgact agaataataa gtactgcaaa acaaagagta agaggacggg agaatgcatg
     9841 atggagtttc agttagacca agagtggcgg ttacgtccca tcaaaggcga cacaggaaaa
     9901 acatacatag gcatgcgtga tgaggacaaa gtctttatta aacgaaacac gactccgatg
     9961 ttggctgctc tttcaaggga aggaattaca ccaaaacttg tttggacaaa acgaacgggg
    10021 aatggcgata ctttgacagc acaagagtgg ctggaaggtc gcttgctgac tcctaatgag
    10081 attggtcaaa gaaacgacgt gattgatgtt ttgtaccatt tacatcattc aaattctctt
    10141 aaaaccatgc tgacccgtat cggtggtcgt gtcatgacgc cggagaagat gctgcaaaaa
    10201 tatgaaaaag aattaccgga agaactccag aaaaatcaat ttctgcaaat tgtccatcgt
    10261 tttcttcgtg agcagatccc gtcaatagat aaggaaaact tatcagtagt acacggagat
    10321 gtaaaccatc gtaattggtt agtatgtaaa aattatttat atctagtcga ttgggattca
    10381 atcatgtttg cagaccctgc ggtggatatt gcaacgattt taggaaatta tgtgccactg
    10441 tctagctgga atcagtggct gatcagttat ggcatacgcc cgactgacga aatgcttgaa
    10501 aaatttcatt ggtatgcggc catgaatttg ctccaagaaa tcaccagata ttgcaaacgt
    10561 gaagatcatc gacggatgaa cgaagaaatt ttacaattaa aacgaatttt cagtggctga
    10621 ggtttggccc aaactctgcg gagatttggc cagacctttt taattagagg aaaggaatgg
    10681 agataaataa tgcgtgtccg aaatcgtccg ggagcagcgg aaatgttagc ggctcacccg
    10741 aattttgtaa ttagtgatcc aacattgtgg aagggaaaat ggaatgaatt gtttgaaaac
    10801 gatcatccaa tccacattga gattggtatg gggaaaggcc agttcatcac tggaatggca
    10861 aaagcccatc ctgaaatcaa ttatattgga gtagaaatgc aggtcagtgt tgtttcgatt
    10921 gcactagaca agttgatcga acaaccgctt cctaacctga aacttctaca tgtggatgga
    10981 tcagcgttga cagagtattt cgctgacagc gaagtagatc aaatctatct gaatttttcc
    11041 gatccatggc caaaaaaacg tcatgaaaaa cggcgattga catataagac ttttcttgct
    11101 gtagatgaac agattcttcg tccaaatgga gaaatccatt tcaaaacaga taatcaagga
    11161 ttgtttgagt attctctggc aagtttttct caatatggca tgatcctcaa acaggtatgg
    11221 ttagatttac atcaaagtca atttgaaggc aatattatga cggagtatga agaaaaattt
    11281 tcttcaaaag gtcaacgtat ttatcgagta gaagctcaat ttcaagataa gaccaaaaag
    11341 aaaagtctgt gaaactaact ttctgctttc ataaaaacat taatatccat aaataaatct
    11401 cagaaaatga acattacaga tgtaatgtat tttctgggat ttatttgatt taaaatgtat
    11461 ttatttcatc ggtttttcca caaggtggaa tcaattggaa gcgagaaaat gaaaataatc
    11521 ctttgtcggt gatcttcaat tcgggtatga cagataatgt taagaaagaa agtgtcaaaa
    11581 aaggatcaaa ggttttagct gctcctagtt gatgtgcttt agttattaat tcattcaact
    11641 gttcatagac gatcgaaaaa ggttcctgtg tcatgattcc gcctatagga agagaaacac
    11701 aagctatttc ctgctgtttg ttgacaacgg tcatcccgcc acctttttga atcaaaagat
    11761 tagcagcata cagcatatcc tcgtcgtttg tcccaacgac aactagatta tgcgagtcat
    11821 gggcaatcgt tgtcgctatc gctccttgtt tcagctgaaa tcctttcact atgcctaatc
    11881 caatgtttcc cgtttgatgg tgacgctcga tcaccgcaat ctttaattgg tctttttcga
    11941 ttgaaggaat gaatcgttcg ccttgtcggt ccactttttc taccaaatct ttagtcacta
    12001 gactattcgg gataatttcg atcacatggg cttgtgaaga agaaagaagc agctctaacg
    12061 aatgtggatt taatggttga gcgtttagtt ttggcagaca acacgtatat gaatttgaat
    12121 gatcagcaaa taaatggtct tggagttgcc cagattgaac aactaatgtt cccttataga
    12181 aaacttcatg aattggcagc ttggtaagat cgtctgttaa gaagaaatca gcttgatacc
    12241 caggggcaat cgcacctagt gttttcaaat caaaacattc tgctgcattc aatgttgcca
    12301 tctgaatggc ttgtaatgga tcgattccaa gtgcaatagc ttgacggacg atatgatcga
    12361 ttgatccttc ttttactaaa tcattgatca gtttatcatc ggtgacaaag aagcatcttc
    12421 ttgaattttc aggtgtgatc gccggtagta gcgatgccag atcttttgca acggtccctt
    12481 ctcgaaccat taaatacata ccaagatcca aacgttcttt agcttctttt gcagttgtag
    12541 cttcatgatc cgtccgaata cctgctgcag gataaacatt taactcttcc atgccgatac
    12601 cagctgcatg cccgtcaatt tttttcttat gctggtgcat caattggatc ttttctagga
    12661 tgtctgtttc attatttgct actgcttgat agttcatgac ttcagctaag cctaaaactt
    12721 cgggtttttg taaaaaaggg gctaattttt ttgcatttaa atttgcacca ttggtctcaa
    12781 aaggggttac aggtacacaa gaaggtagca taaaaaagat attcatgagt gtttgtcttg
    12841 catcttctat cataaactca agacctgtac tcccagcaat gtttcctatt tcatggggat
    12901 ctgtcactac agtagtaact ccatgaagca aggatacttt tgaaaattcg cttggtgata
    12961 aaagtgagct ttcgatatgc acatggccat cgatgaatcc tggcactaga tattgcccat
    13021 tagcattatg tacgcttttt ccttcgtatt caccgattcc tacgatcatt ccttcgcaga
    13081 ttgctacatc tttttccatg atttctttcg taaagacgtt gacgatctta gcatgcttga
    13141 tcactaggtc agcaggcttt tttttacttg ctacttcgat atatttcctt aatgttgttt
    13201 ccaatggagt gcctcccaag ataaattttt ataagtataa cataaattcg gcttttgcca
    13261 atattttttt gtaaattcat tttatttgaa acaaaaagat caaaaagacg tatgtttttt
    13321 gtggagggga gaataatgga aataaaacga ttatcggatc aaatgataag tgaaagtttt
    13381 gccaaacatg cccaagacgt atatatattg ccagcttcag aagtatttta catctatttt
    13441 cgctatggaa aaaaacgaac attctggaag aaagtctctt cggcagatgg acagcaactg
    13501 atctcaagat ttaagtattt gggacagatg gatatcggag aaaaaagaaa agctcagtta
    13561 ggtgcattta cttattcttt agaaaaacag gaagttcgtc tgcggttgtc ttctgtagga
    13621 gattaccttc aacgagagag tttagtgatt cggctattat acggtacttt tgaaacaccc
    13681 catcgttgct ttagttctcg agattttgca actattttac agaaaaccgt cagacgtgga
    13741 ttataccttt tcagcgggac agtcggatca ggcaagactt ctctaatgta tcggttggct
    13801 ttggaagaaa aattgcaagt gattactatc gaagatcctg tagagataga agaaccgttt
    13861 tttctgcagc tacagatcaa tccgaaaata gcacagactt atgatacact tttgaaactc
    13921 gcattacggc atcgtccaga cttgctaatc attggcgaga taagagatga atcaactgca
    13981 catgctgcgg taagagcagc actaacaggg caccgggttt ttgcaaccgt acatgcacgc
    14041 aatcttgaag gtacattggt cagaatccat gaactgacaa agaaaaaaga agagctatct
    14101 gaatgtttag ctggtgtcat ttatcaagaa cttttggaag atgccaatca ccatccttct
    14161 gttttatgga gttatcaatt tatggaggaa gaatcagtaa aaaaatcatg ggaggaaagt
    14221 tatgaagaag ctcaggaaaa tatttggcag tcagaactat tctaaaggat taacaagaag
    14281 ccagcaaaac cagtttgcac aaaccatggg agaactgctt ttatctggtt ttagtctgca
    14341 agaatccctt tatttattac tcacgatccg ttcgtttcca cctgttattt tggcaaatat
    14401 ccaacatcag ctaaaggaag ggcaagcgtt tagtgaggta ctttcgcaaa taggatatgg
    14461 agcagagcaa ctggtacaga tcgagttagc tgaacgtcat gggaatctgc cgacaacttt
    14521 gttggaaatt gccaaacagt ttcggcttgt tgaaggattt cgcaatgatt tgaaaaagct
    14581 gcttgcatat ccgatgctat tgctcacctt tttgctaggt atcttgttta gtatgcggtt
    14641 gttcgttttg cctcagctta ttggttcagg aatggtcatg caagagcatt ggggtattca
    14701 gctgatacaa gtctttcatt ggtatgttct actattcatc ctagcggtta ctctattggc
    14761 tttactagta aggtatcgtc tgatgaagtt gtctcctgtt gatcagagtg aatggataag
    14821 cagacaatta ttgattggac cgatatattc cttgtatcaa tcggcttatt tgtcactaga
    14881 gattggaaag ctttttcatg aaggtttcga attaaggcaa atcatagtct gcttaaagga
    14941 aacgaaacaa gaaagtctga ttcagcatct tgcctctaag ttgattcaag gattggaggc
    15001 aggtatgtca ttggctgagc agttccagaa ttatcgtttt ctatcagaag aattcagtaa
    15061 aattgtttta cagggagagg caaagggaag tttaggtaaa gaactgattt tttacagcga
    15121 atggacaaga aaacagcttt tcaaacgtct tcatcactgg atgcatttta ttcagccaat
    15181 catttttttg ggcgtagcgg ctttgatttt attagtttat gccgctgttt tattacctgt
    15241 ttacggaagc atagaggagg ttttgccatg aaaaagagaa aaatatctta ttatagcggt
    15301 tttacattga tcgaaatgct aatcgtctta ctgatcatca gtgtattagt gttgttgttc
    15361 gtccctaatt tgtcgcgtta taggaatcat gtagaccaag aaagtcgtga agcgatcatc
    15421 cagttggtag acacacaaaa agagctctat gcgcttcaaa ataatggaag agttcctact
    15481 gtggaggaat tgttgaatga aggctatatc aaacgagaac atgcagaaat ctatcagcgg
    15541 ccttgaaggt aaggttttag catcaggata tacgttgttt gaatcactct tactgatttt
    15601 attacttaca acgatccttt cttttccagt tctcgcattt tctgcatgga agaatgaatt
    15661 ggcagtctat caatttttcg gccaatttga aaaacgaatc tattctacgc aaaaaatcgc
    15721 tattgtgaat cagatccaaa caggctttta ttggaatagt gaagagaatc aaatcatttt
    15781 tcatgttccg aatccagata aaataaattg ggaagtatta gatattcctg atgagattac
    15841 cttgaaacgc catgcttcta ttacttttgc tgctagaacc gggaatgaaa gttcactaaa
    15901 agcttatcaa ttttattggg aagaaaaaga gcggacgatt acgtatcaat tccagatagg
    15961 gagtggtcgc tatacaaaaa gaattgaata aaacaggatt tgttttgttg gagtccctta
    16021 cagcgcttat gatcgcttct gtctgtgtct acttagtgag ctttggccag ctgtttttgc
    16081 ttcaggcaga aaagaaaagt ggggaggaac tgctatttat tcgaatgatt tatgaagata
    16141 tcaagacgca gcggatgtat cacaaaagac cagagcgaag ggaagaaatg atcccatacg
    16201 atatccaaat tgtcgataat aatggagtaa tcgaaaaagt gtttgtcaat atgtagaaag
    16261 aagcgatcag tattgaaaaa acaagtaatt ccagcattta cgctgattga atgtctaatt
    16321 ggactgatga tattgagcag tctattgctc acattcaatc ttttgattca gcaaaccatc
    16381 caagtaagtg aatttttgca gcgtaaagat caaaaagagt ggctaatatt tttagcacaa
    16441 ttagaagaag aactcaagag cagtcacaat gtctctgtga aaaatcagca actgcactac
    16501 acgatcgagg ataaacagta tatcatcgaa agatatcagg caatgatacg taaaagaaga
    16561 acttccggcg gacatcaacc tatgttaaca agtatcactg agcttcaatt attggaaaaa
    16621 gaacacacaa tttcttttta tgtacacttt gaaaatggag aagatggata tgccatatgg
    16681 acaaaaaacg atcagtagaa aatgttataa gggctctatt ttattttctg tcttgctttt
    16741 atttctttta tgtacgtttt tattgatggc agcattagaa agctaccgtt tgtcaatgga
    16801 tcttaatata cgaatcaaaa attattatat agcaaaaata atgaagcaga tggctttatc
    16861 ggaacagtct acgcttgccg aaagttcgga aggagttttt tactacacaa ctggatctgt
    16921 aacatatcag tgggtacaac agtccctatt cttagaggtg gaagtctcac cgtttatctt
    16981 tcggttcaca gaagaagtaa aaaatgatac agatactagt acagaatgat ggaatgttcg
    17041 tgaaaacgcc ctcttttctg ttataacact gtttttaata aaaaaatgtt tgccgtttca
    17101 caaataaaaa acagtgaaaa cgattgaaat tttgtcaatt gttcgataga attaaacagg
    17161 tgaaaaaatt gttataatat atctagaaat gaggtgcacc atgttaccag aaaaaatgga
    17221 acaagggttt aatcaaaacc tggaagctat tcagctttta cagaatgcat tgggaacttc
    17281 ctttttagaa gcctatgtcg aaaatgcaga aaatttaata gatgattatc aagtacgtgt
    17341 ggtggatgga gtgccgacaa aagagacaac gcaacggatc acagctcttt atgaagagtt
    17401 gaagaaactt tctttcgagc cagaagaatg gcgcagactg tctcaattac ttttattgaa
    17461 gggcagccag acagaacatt tgcaggcgaa ccatcaactc actcctgata gtatcggttt
    17521 tttatttgtt tttcttattg aacaactata cacaaataaa aaagcaccgg ttaagattct
    17581 ggatattgcg gcaggaatgg gcaacttgtt attaacagtt cttttaaacc tatcgaatgc
    17641 ggggtaccaa acagaaggaa ttggtgtgga tattgatgat acattgttgg ctgtcgcagc
    17701 ttctacaagt gatttgacac aagcaaatgt tcaatacttt catcaagacg ggttgcagga
    17761 attattaata gatcctgtcg attttgcaat cagcgatttg ccaatcggtt attatccgaa
    17821 tgacgaaaaa gcaaaagagt ttttaacaag taccgaagaa ggccatagtt atgcacatca
    17881 tttattgcta gagcaatcaa tgaaatatgt gaagccggat ggttttggtt tgtttttgat
    17941 gccgagtgga tttttagaaa cggatcaaag tgaagaaatc aaaaaatggt ttaaagaaga
    18001 aggttatctt caaggaatga ttcaattgcc agatgaattg ttccgtaata aacaatcgca
    18061 aaaaagtatt ttgatcttac agaaaaaagg accacaagca caacaagtaa aagaagtctt
    18121 gcttgtgaaa cttgcttcat taaaagaacc agagaaggtg acggaatttt tcaacgagtt
    18181 taaaaactgg aaatcatctg ctctgtaatc attcaacgag aaggagagtc atatgtctaa
    18241 aacaattgca atcaatgctg ggagttcaag tttgaaatgg caattatatc aaatgccaga
    18301 agaaaccgta gttgcgaaag ggatcgtcga acgtatcggt ttacaagatt ctatttttac
    18361 catcaagtac ggcgataatc aaaaattcga acaaatcatg gatatcgaaa accacgaagt
    18421 agcagtaaaa atgctgttag atcagttgat tgaactgcaa attttaagct cttatgatga
    18481 aatcaccgga gtagggcacc gtgttgttca cggaggagaa acttatgggg attcagtagt
    18541 gatcgatgag aaagtaatgg accgcattgc tgaattagca gaatttgctc ctttgcataa
    18601 tcctgcaaac ttaatgggga tcaaagcgtt taaaaagatt ttacctgaca ttttgagtgt
    18661 ggctgtattc gatacatctt tccactcaac aatgccaaag caaaattatt tatatagctt
    18721 accggtgtct tattatgaag atttcaaagt aagaaaatat ggcttccatg gcacaagtca
    18781 tcgttatgtg tcggaacgtg cggcaaaatt attgaacaaa ccgatcgaag aactaaaaat
    18841 catcacttgc cacttaggaa atggtgtatc gatcacagca gtcgatggag gtaaatccat
    18901 ggatacttct atgggattca caccattagc tggggtgaca atgggtacac gttcaggtga
    18961 tatcgatcca gctgttctcc catatttaat ggaaaaatta gagattgata ttgatgaaat
    19021 gatcaatgtt ttgaacaaga aatctggtct gctaggccta acaggattgt ctagtgatat
    19081 gcgtgatctt gaaaagaact atgaaaaaga acatattcgt ttagcttatg acattttcgt
    19141 ggaccgcatc cgaaaataca tcggcggtta tgtgacaacg atgaatggtg ttgatgcaat
    19201 tgtctttact gctggtatcg gcgagaatga cggacatgta cgtagtgaaa tcatcaaagg
    19261 tatgtcttgg tttggttgtg aaatcgatcc agcgttgaat gaattgcgtg gtgaagaagt
    19321 agatatctct acaaaagaat caaaagtaag agtattagtg atcccaacag atgaggaact
    19381 aatgattgcc cgtgatgtgg agcgtctgcg cggctaagcc aaagcattcg ttaggataaa
    19441 agatgaaacg catgcaagag ggaattcttg catgtgtttt ttttgcggag aagaccgcaa
    19501 aatggtaatt taacttcaat ttcccttctt ttttacagga tcttcttagg gaattttgct
    19561 tggaaaagtg gtcaaaaggg aaaagactgg cattttattt attgattttc aggaaaatcc
    19621 caatatttta ttttcattca tctttataag ggacattaca tatgtaaaaa gaaaaaacat
    19681 ttttagatca gcctcgtggg ctgtcgactt tattcttcgc tttatgttta gctagaaaac
    19741 cgatccgaaa cattcattga tcgaataata aaaacttgat tttccacctg tactttgcct
    19801 gaatcgtgaa agcacaggta tgaaaatcaa gttttttgtt gcgaaaaaac gtcctagtca
    19861 atcagttggc cgagcggctg gcggctgatc tgctcctgat caataacgag tgcttcttgg
    19921 aagtgttgct gcaaaaagta gcggtcatgg ctgactgcta ggagcgttcc ttgatagtct
    19981 tgcagcattt cttcaatttc ttctcgagca tagctgtcga ggtgattggt tggttcatcc
    20041 aaaatcagga cgttgatttt ttctaaaaag agtttcagca acgccaaacg gattttttca
    20101 ccaccggata aatctttgat ccgtttaccc acatcgcttt gataaaagcc acttttggct
    20161 aattgccgtc gagcgacttc ttcgtttcca gtcaatgtga tggcgtaagc cagcaaacgg
    20221 ctatcgggat cggcaaactg cagctgctgc ggcaaataac cgatttgcaa gctactacct
    20281 aatttgatgg tgccaccgta aggcttttcc tcaccgagta ataatttgag taaggttgat
    20341 tttcctgcac cattgtcgcc gatgatggct aaatgatctc cacgatagac tgcaaaactg
    20401 ctgtctttga atagtacttg atcagcgtac tgctggtgca agttttgaat gatgaaaact
    20461 tctttgccag attttccacc attagacaag ctttgcagcc gatttttcgg gggaggtggc
    20521 ggtttgacga gtgtcagttt cgccaggcgg cgttccagtt ctttggcttt tttgaagaag
    20581 gactcattgt ctccttcatg tgcccactga cgaaaacgac gaatcatgac tttcaaccgc
    20641 tggacttctt tttgctgcag ttcgtaattt tttgtcagct cagccaagcg agcttctttc
    20701 aagactcgaa aacggctgta gtttccggga tactcaatca atgcaccttc ttccagttcc
    20761 catgtttttg tggtgacttg atcgagaaat tgccgatcat gagaaacgac cagaaccgat
    20821 ttttttgtat tctgtaaata gttttccaac cagcgaatac tgttaagatc taaatgattg
    20881 gtcggttcat cgagtaaaag taaatcggca tcggctgcta aaattttggc taaagcgact
    20941 ttaactcgtt cgcctccgct aagttctgtt aaccgtgctt cctgacagtg tggaatccct
    21001 aagccttgca agatcgctgc aatccggtct tctaatacgt aaccgcctag ttcttcaaag
    21061 cgatgctgca gctcaccgta acgggccaga cacttctcta agtttgtatc tggttctgtc
    21121 atcaatgctt cgtagcgact catttgctgt ttgaggtcct gtatctcagc ggcagaatgg
    21181 agcaagtagt ggttgacggt cgcttcggaa actgtaagtt cttgttcgac cgtcccgatc
    21241 tttaagcctt ttttgcgact gataacaccg ctgtcgacac cttctctgcc ggtcaagatt
    21301 tgtaaaaagg ttgatttgcc agttccgttg atatcgacta aggcaatttt ttcaccagct
    21361 tcaatttgac aggataaatc ctcaaataat ggattcccgc cgattgtttt tttaatagaa
    21421 attgcttgta tgatcatgat ctctctcctt gagaccactc aaaaaggcca tggttatccc
    21481 tggcctgtgt tatttaaact cagattatca tgagaaaaat accttgagat ggccattgat
    21541 tttacgtgaa ttcaactgtt ttggacaaac ttgtcccgtc aacagcaact gtaaaaccaa
    21601 ttgcatggct aaatgcgata tggtatcgtt ggtaattttc attacttgtt attttagtca
    21661 tgcgcatcat agtattttcc ctccttcgtg tagttgtctt ttccagtgta cctgaaagtg
    21721 tcagaaagtt caactagcgc tgacatttac ttaatattta tcgatgtgta attctgtcag
    21781 agagcttttt ttatctgact gagggtaatg tctcgtggac cacatcattg tattctgagc
    21841 taaaccagcg ttcaggcgaa atcatatggt gtttgataaa aaactttgtc tacatgactt
    21901 ccttttgcca tttgcttaat accgttgcat taaacagctc ttctctttgg aaatctcacc
    21961 aacatcgcgt tcaggagtat cgttgtggtt gcaatggcaa gcggtagcct ttcatcaatt
    22021 cttgaatgat ttaccgaatg tagcacttgc cttctcgcgg tacccgaacc cgaaataaaa
    22081 ttttagtttg gcgataaaat cggtatccgc catcgatact tcatattttt tcgtatcaga
    22141 ttcatcggaa acttccacat tgacgaacca ataagctttg gcatgcttag gtcgttttaa
    22201 accacaccag ggcgactaat cgccctggtg ttcacttttt ttattttact tgaactcatt
    22261 gactgttttc cctttatttt aaacattata aagaccgatg agtgcttgcc gcgtcttttg
    22321 tttttatcac ggttttttgt gcaaaaaaac ctgccgatag ttggcaggtc gtctacgtgt
    22381 taagctttgg tgtggtcttt agctggcaat tggttttcta aatcagttcg gacaacaagc
    22441 acatcgcaag ctgcgtttct aataacatat tcagaaacag aaccaataaa taaacgttct
    22501 acagcattca atcctgtggc tcctaacata atcaaatcta cttgattatc ttctgggatt
    22561 tctctggcaa taatttgttt tggtgagcca tattctacga cagaggttac attattcaag
    22621 ccagctttct tcgcattgct ttcataatct gccaatgttt gctttgccat ttcagtagct
    22681 tgttctgcaa gcattccatc aaaggatgaa acggtttgga aagaacgtgt gtcaatgaca
    22741 tgtgctaata gaagttctcc gttatttcgg atagctacat tgaccgcctt tttaaaagct
    22801 aattcagctt catcagaacc atctacggca accatgattt ttttgtattg ttgtaacata
    22861 agcattcact cctcaagtca atcgtattgt actcttttat tgtagaacac tttagggaaa
    22921 aagagaatta tttactgctt tggttcagca attttttttg caaatcggat actgaaggtg
    22981 gcagaaacaa taattacgat cagtaaaaag accaaggcaa taaagcgttg attgaaaaaa
    23041 gctgttaaaa tacccattac gcctaaaaca gcatatgcag caccgtaaaa ttgtaggaat
    23101 ccttgatttt tatcattttt gttgatcaat acaaaaaaga tatcctgttt attaaataaa
    23161 taatagctaa cgaataataa tactaaagtg gcgataatca ttaaaatcct aatcatgaac
    23221 aaatcctcca aaataagcaa caaatagaaa aagcaatttg ccaagagcaa attgctttat
    23281 tctttaaaag atggtaatct ccgttaatgc cgttgtttgt cctgtagtat tgatcccgcg
    23341 aattcaagca ggtgggtccc atggtcgtag cttcgaacta ccaaatgata aggcatgtta
    23401 ccagcagtga cacaagacgg gtcccaagat atcaataaaa atgttcggtc aacacacaaa
    23461 gaggacaatt cgaacatcac agatttccca cctctatagt agcatatgaa tttaaaaaga
    23521 gcaatgagag agctcgcaga aacagaaccg ttttcttgtc tagttttttg gaggatgctt
    23581 tttccactct tttatacggt aatactgttg acccagagcc tgttcatatt tccctgtatc
    23641 ttttggctga taatactgag cattcttcag tttatctggt aaatattgct gattgaccca
    23701 agcctgatca aaatgatgag gatactgata acccacgcca cggttgagtg atttcgcacc
    23761 tttgtaatgg ctatctctca aatgatcagg aacatctcca gctttgccct ctcgaatatc
    23821 agcaagtgct gcatctaatg ccatgtaggc agaattggat tttggcgaca agcaaagatc
    23881 gacgacgacg tctgctagtg ggatacgtgc ctcaggcaat cccaattttt cagcagctaa
    23941 tacagcgttc actgtacgag ccgcagcagc aggattacct aaacctatat cttcataacc
    24001 gataaccatc agacgccggc aaatacttgc cagatcacct gcctcaacta aacgggcgag
    24061 ataatgaagt gcagcatcca catcacttcc acgaatcgat ttttgaaaag ccgaaataac
    24121 gtcataatga gcatctccat ttttgtcatg ggtaagagct tttctttgga cacattcttc
    24181 aatgatcgac agtgtaagat gaatccttcc ttctttatct gaaggagtcg atagtgtagc
    24241 cagttccagc ccattcaatg cactccgcaa atcgccgttc gttgcacgcg aaaggtgaag
    24301 aagagcatct tcgtctaact ggattgcttg ttgtccaagg ccgcgttctt tatcccttaa
    24361 agcatgttcg actgccaact ggatgtcttg ttctgttaat ggtttgactt caaagatctg
    24421 tgtacggctg cggatcgctg gattgatggt aatataagga ttctcagtag tcgctccgat
    24481 caagatgatt cggccacttt ctaaatgagg aagcagaaaa tcctgttttg ttttatctaa
    24541 tcggtgtact tcatccaata agaggatcac tgtaccactc atttttgctt cttccgctac
    24601 aacttgtaga tccttttttg aatctgtagc ggcgtttagc atgcgaaatg cataattagt
    24661 agagccggcg atcgcacttg cgatactcgt ttttccagta ccaggaggtc catatagaat
    24721 catggaagag agcatgcgag catcgaccat gcggcgtatg atttttcctt ccccgactag
    24781 atgctgctga ccaacgactt catcgatcgt tcgaggtctc atgcgataag ccaatggctg
    24841 ttgcatagga ttttcctcct tcttgttttt acgtccctta attatatcat atttagcaag
    24901 atgcctagaa agaacagcct tctgttgttt tgttttcata gcttatccaa ctaatattgt
    24961 gtatgataaa gtaggtgaga aaaatgacgg ataaacaaaa gatagatgaa tttttttgga
    25021 ataaaacgac gccagaagtg gcacgggatt tattggggat gtatttggag catgacacac
    25081 cagaaggaag attagctgga tatattgtag acgcggaagc atacctaggt ccagaagacg
    25141 aggccgccca tagctatggt ttaagaagaa caccgcgtgt gcgggcaatg tatgaaaaac
    25201 caggtacgat ctacctttac acgatgcata ctcatcggat tttgaacatc attactcagc
    25261 cagaagggat tcctcaagga gtaatgatac gtgcaatcga acccgccgca atgattgatc
    25321 aaatgagtaa aaatcgtggc ggaaaaactg gtccagatat cagcaacggt ccgggaaaat
    25381 tagtggaagc attagcaatt cctcaagaac tttatggtca gtctatagca gatagttcgt
    25441 tacgcttagt ttttgagaaa aaaaagacac caaaaaagat caatgctttg cctagaatcg
    25501 gtatcccaaa caaaggtgta tggacagaaa agccgttgcg ttttgttgtg tcgggaaatc
    25561 cttatatctc tttacaaaga aaaaaccaaa tcgagaaaga ttggggttgg aggaaagaaa
    25621 atgaaaaaga aggatctatt aacatattta gatgaaataa ttgaaaaaca agtgacagac
    25681 tatgatgtag ctttagattg ggatacaaaa aatcatacga tcgagatcgt tgttcgcttg
    25741 tttgcagaaa ataaagcaca atttgagatt gatgatgcag aagggatcgt ttcgcaagaa
    25801 ccaattatcg aatttgaaga cggcgtactt ctttttaatc ctcagaaatc tgtttttgat
    25861 gaacgcaatt atctagcagt aatcccttat gaaggaaaaa aagggttggc aaaatcagta
    25921 gctgattctt tagtggaata tctaaatgaa gtattagctc aagggcaaag cgatttgctt
    25981 gattttttgg atgaagacga tgacgaagct gtattcgagc ttcactggga taatcaacaa
    26041 ctagcagaat tagtagaagt aaaacagcaa aatgaagcag atacttattt accgtaccca
    26101 agttattaag gagtgacaga tatgaaatgg aatgaagtaa aagtagaaac tgaaagtgaa
    26161 gcagtcgaag cagttgctaa tattttgatg gaggcagggg cgagtggtgt cgcgattgaa
    26221 gattctttag atgttgaaca ttttcagtcg gatcaatatg gtgagatttt agataaaaat
    26281 gattttacat cgctgaaaga aggcgcacta gtcatggctt attttccgga aacgactttc
    26341 ttaccggaaa tcttgccttt tatcaaagat cgtatcacac aattacctga gtttggtcta
    26401 gctattggaa aaaataaagt aacagttagt gaagtagagg aaagcaactg ggcaacagcc
    26461 tggaagaaat actatcatcc ggtgcgtatt acgcgaatgc tgacggttgc gccaagctgg
    26521 gaaaactacc agacagaaga tcctctagaa aaaattatca cactagatcc tggtatggca
    26581 tttggcacag gcactcatcc cacgacttcc ttgacattga cagcactaga aacagtcttg
    26641 cgtggtggtg aaacattgct agatgtggga actgggtcgg gcgtgctaag tattgcagca
    26701 aaatatcttg gtgcaaaaga tgtctatgcc tttgatttag acgaagtagc cgttcgttcg
    26761 gcgaaagaaa atatggatat gaatgaaata gccaaagatg tccatgtatc agctaacgat
    26821 cttttgacag gaatcgagat tgaaagtgat gtaatcgttg cgaacatttt agcagatatc
    26881 atcttattga tgatcccaga tgcttggcgt ttactgaagc aaacaggcac gctgatcgtt
    26941 tcaggaatca ttgaagagaa gaagcaaatg gttctggatg ccatgttaga acaagggttt
    27001 atcgtggatc aaattttcca gcaaaaagac tggtatgcaa ttattttgaa aaaaccagag
    27061 gtggaataga tgcagcggta ttttttaaaa gaatcatatg aatcaaaaga ctattttgtt
    27121 ttagaagggg aaaactatca ccacattgta cgggttatga ggatggaacc cgaggatgaa
    27181 gtttttttgg cattcaatga tcaaatagcc attgttgcag aaatcacgga gattactgaa
    27241 gaggctgttt atctgaaaga aattaaaaaa gaagaaaatc aaaaggaact gccaagttcg
    27301 attaccatag cgagtggcta tcctaaaggg gaaaagctgg attggatcgt tcagaaatcc
    27361 actgaattag gagcgcaagc ttttcttggt ttcccggcat cagcctctgt cgttaaatgg
    27421 gatgcaaaaa aacgtatgaa acgacaacaa cggcttgaaa agattgctca agaggcagcg
    27481 gaacaatccc atcggcagat cgtgccatca gtagagttat tggaaacgaa acaagattta
    27541 tttgagcggc tagatgatta tgacgtggtc ttggttgctt atgaggaatc agcaaagcaa
    27601 ggcgaaaaat caaatgcagc caaaattctt agtgaaacaa aaccagatag tcgaatctta
    27661 gctgtctttg gaccagaagg tggattatcc cgcgatgaag tcgattattt tgtagaacac
    27721 aaagggatac tttgtggatt aggaccaaga atccttcgta cagaaacagc acctctttat
    27781 ttattaagtg ctgctagcta tcaatgggaa ttaatgaatc cttctttcag ctaacgttct
    27841 tcattaataa aagagtatcg acagaaagaa ttgaaaaaat agtcaacaat ttgtataata
    27901 gaaacaatta tttatacaat ggatacgaaa aatcgtttga aaaagctttt aaaaagcgaa
    27961 catttccgta aaaaaacgta taactgcacg gattttggga caattatcaa acagatattt
    28021 gtcccgattc ccgttttacc gaaaaaagaa agggggatcg attcctgtga caaaagaaga
    28081 aattttaacg ggacccggtg tcataaagct cgttagtcaa tacatgggtc ccgaacatgt
    28141 agcatttgtt gagaaagcat gcgaatacgc gactgctgca catgacggac agtttagaaa
    28201 atcaggcgaa ccctacatta ttcatcctat ccaagtcgca ggtatattag cagatttaaa
    28261 aatggatccc catacagtgg ctacaggctt cttacatgat gttgttgaag atacagaaat
    28321 cactttagaa gatctgagag aagaatttgg cgatgacgtt gctatgttag tggacggcgt
    28381 aaccaaatta gggaaaataa aatataaatc ccacgaagag cagctagcag aaaaccaccg
    28441 aaagatgctg ctagcaatgg ctcaagattt acgagttatc atggtcaaat tagctgatag
    28501 actacataac atgcgtacgt tgaagcacct gcgagaagat aagcaaagaa ggatcgctca
    28561 ggaaacttta gaaatctatg cgcctcttgc acatcgtcta gggatcagcc ggatcaaatg
    28621 ggaattagaa gatacagcac ttcgttatct aaatccaaaa cagtattacc gtatcgtcca
    28681 cttgatgcag acgaagagag aagaacgtga aaaatacgta agtggtactg ttgaagatat
    28741 tcgaatagcg acagaagagc tggggatttt tgcagaaatc tatggacggc caaaacacat
    28801 ttattcaatc tatcgtaaaa tgaaagatca gaaaaagcag ttcaacgaaa tttatgactt
    28861 gctagctatt cgagtgatcg tagattcaat caaggattgt tatgctgtat taggagcaat
    28921 ccacacaaaa tggaaaccaa tgcccggcag atttaaggat tatattgcca tgccaaaagc
    28981 taatatgtac caatctttgc atactaccgt gatcggtccg gcaggaaatc cggtagaaat
    29041 tcaaatccga acacaggaaa tgcatgaaat cgctgaattc ggggttgctg cacactgggc
    29101 ctataaagaa ggaaaaaatg aaaaagtaga accagatggt atgacgaaac aattaagctg
    29161 gttccatgag atactcgaac ttcaagacga aagctatgat gcttctgaat ttatggaagg
    29221 cgtaaaagga gatatcttta gtgataaagt ctacgtcttc acaccaaaag gagacgttac
    29281 tgagttacca aaaggatccg gaccattgga ctttgcatac agtatccata cagatatcgg
    29341 taacaaaacc actggtgcaa aagtaaatgg caaaatggtg cagcttgatt acaaattgaa
    29401 aaacggagat atcattgaga tcatgacttc tccaaattca tttggcccaa gtcgcgactg
    29461 gttgaaatta gttgctacta gcaaagcaag aaataagatc aaacgtttct tcaaagccca
    29521 agatcgagaa gaaaatgtga tcaaaggcca cgaatccgtg gtcaaatgta ttacagatct
    29581 aggatttacg cctaaagata ttttgacgaa gaacaaactg caagaagcac tcgatcgttt
    29641 taattatcaa acagaagatg atctctatgc agctgtaggg tatggagaag ttagcccctt
    29701 gacgatggcc aatcgtctga ctgaaaaaga acgtaaagaa caaaaaatcg agcagcaaaa
    29761 gcaagaagca gaagaaatca tgaatcagcc gaaaaaagaa cctgacaaaa tgaaagtacg
    29821 tcatgaaggt ggcgttgtca ttcaaggggt agaaaactta ctaattcgta tcagtcgctg
    29881 ctgtaatccg attcctggtg atgatatcgt tggttatatc actaaaggca gagggatatc
    29941 cattcatcgt cgagattgtc cgaatgttca gcctgacaaa ccaaatgtag cagaacgttt
    30001 gattgaagtc gaatgggaag atacatcgaa tacacgaaaa gagtatgatg cagatttgga
    30061 aatttacggc tataatcgtt caggcttatt gaatgatgta cttcaaacag tcaatgcgct
    30121 aacgaaaaat ctcaacagcg ttgaagcacg gacgaataaa gataaaatgg cgacgatcca
    30181 tttgacggtt ggtatccaga atttatccca cctaaagagt atcgtggata aaatcaaagc
    30241 agtacctgat gtctacagtg tacgccggac gaatggatag gaggacagat atgagagcag
    30301 ttgttcaacg ggtaagtaaa gctgctgtgt cagtcgatca gcaagaaatt ggaaaaattg
    30361 gctacggcct acttgtttta ttaggcattc atgaaaccga tacgcaaaaa gatgtggact
    30421 atctggtaaa aaaaattgca caaatgcgta tttttgaaga tgaacaagga aaaatgaata
    30481 ttagtgtaga agatgtggga ggacaaattt taagtatctc tcaattcaca ctgtttgcgg
    30541 atacgaaaaa aggaaatcga ccaagctttg tggaagccgc acgaccagaa acagctattc
    30601 ctttatacga atcattcaat gaaggaatca gaagtcgcgg catcacagtc gctaccggac
    30661 aattcggagc agatatgtcg gtttctctcg tcaatgatgg acctgtaacg atcattattg
    30721 attctaaaaa caaataaagt aatctggttt tcactagtaa aaactatgaa acacgtcgct
    30781 taagttagac tgataaactc taacttaagt ggcttttttg tctgcaaaaa gcgcttcttt
    30841 tgtcagcagt cggaaaaaaa gttaagctta aattatacaa gttatttttt ataggaggtt
    30901 gctgaagatg aaagaagtac cagtagtaga tatcaaaatc acagatttaa aaaaattgta
    30961 tcaaaaagga tacaacatgt tggaggagtt acgccatgaa gcggacgctc ccgtcgtcaa
    31021 agcaaagatt ttcaataaag aagctatcac gatctacgga tcatcagcag caaaagtatt
    31081 ttatgatcca aggaatttca aacgaaaagg tgctatgcca aaattagtat tgaagacact
    31141 ttttggtcaa ggtggagtac agactttgga tggagctgct catcaccatc ggaaaaatat
    31201 ttttatggat ctaatgacac cggaaagaat ggaagattat catcgtatct tagataagaa
    31261 tctgacacaa gcacttgaag cacagcatgg tcaatttgaa ctatttgatt taagtaagat
    31321 ggttttcttt acatctattt gcgagtgggc aggtatcaat ttatcagcga tttctaaaga
    31381 tgaggtcgaa aaattagcag aatatcaaat ctctatgatc agcggaacgt ttacttctcc
    31441 gattgatcac ataaaaggtg tcgagaatcg caaaaaatca gaaaaatggg cgcaaggctt
    31501 gattgaagaa gctcgacaaa atcctgtggc tggaaaagaa aatgtggcat tatatgcttt
    31561 tgccaatgcc acagatttag atggtcagct tttacctctg gaagtggcag ctgttgaatt
    31621 attgaacatc atccgtccaa cggttgctct aactgtgtgg gcggcattga tgggacatgc
    31681 acttttcagt agaccagatt tgtatcagca attaaaaaat gacttttcga cgttacaaga
    31741 tccgtttatt caagaaatga gacgctatta tcctttcttc ccaatgctac ctgcgatttc
    31801 gctgaaagaa gtagaagtag atggttatcg tataccagaa ggcagttggg ttattttaga
    31861 tttatatgga acagaccatg atgaacgaac agttgaagcg ccagattcct ttatgatcaa
    31921 acggtatgta gggaaagcaa aagatatttc ctataaagaa gaatatgaga tgattgcaca
    31981 aggcggggga aattttcgac aaatgcatcg ctgtgcagga gaatggatca ctcttcatag
    32041 tctacgtgtg ttttccgatc agctagtaaa taagtttgag ttctctgtac ctgaacaaga
    32101 ttggacgatt ccattcaatc agtttccgac ttatccgaat agccgagcgc tactttacaa
    32161 gaattagatt agcataaact tatcgaaaaa aaggaagatc aaacagtgct gatcttcctt
    32221 ttttcatatt actataaatt gataaggtca attgatcaaa atccacgaaa tgcttcatca
    32281 atggtttgat aatcttcatt ggataaatgc aaatctaaag cttggacatt gtcagatact
    32341 tgctctggac gcttagctcc aggtataact acagacactc gtggattctt catgtaccaa
    32401 gctaaaacaa gttgcgcagg agtggcttgg tatcttttag caatggggcg aagtacatcg
    32461 acagcggtta aaatttctct gaaacgttct ccttggaaat ctggatttct ttttcgagga
    32521 tctccttcac caaattgttt ttcctcacca agttcatatt ttcctgtcag caaaccggaa
    32581 gcaagaggaa aatagggaac aaaactgatt ttgttttttt caagataagg gaataattct
    32641 ttttcagcct gccgatggat caaactgtat ttatcttcta ctacatcgac atatccatct
    32701 gcattcgctt ctttcaattg ttctaacgtg aagtttgaca caccgactgc tcggattttt
    32761 cctgcttctt ttaattcgtg caatgtagca actgattcat tttttggtgt gctttcatcc
    32821 ggaaagtgga tataaaaaat gtcgatgtaa tcagtttgca gacgttttag tgcatcttcc
    32881 accgattgtt tgagaaactc aggagaatta tcaaatgttc gccctttatt tggtacatga
    32941 gcagcttttg tagcgatgac gacacgtgag cgatcatatt ctttttcctt cagtacctcg
    33001 ccaatcagtt cttcggaacg gccgtttccg taagcataag cggtatcgat caaattgatc
    33061 ccgctattca aagctgtacg aactacttgt tttcctgtct catcttccaa accgtcaaaa
    33121 agattatgtc ctccaacggc atttgctcct aatccaagct gttctgcgta gacttgtgaa
    33181 tgtccgattc ttatttctgc catagctgat tcctcctttt tgtctagtct aaaacaaaat
    33241 accaaaaagc tgaactaaaa cgtttttgtg aggggaaata gtcagatatc tcttgacttt
    33301 gagactggtt tttagtaggt tataagtaat atcgaatgtc aatgaaagag cgagtaagtt
    33361 tttgaaactt tacaagagag gatcatcgag gctgaaaatg atctaaagtt tcattagcct
    33421 aaagacactc tggagatgga cgagtgaaaa gccgtcccgc gtacgagcgt tattcgtaag
    33481 aggaaagaga aatctttcaa aattaggtgg taccgtgtat gaatttcatt cacccttgtt
    33541 catgatgaac aagggtgatt tttttattta catagctaag aaagggagag aaaactatga
    33601 gtttccaaag accaaaagga acaaatgata ttttgccagg cgaatcagaa aaatggcagt
    33661 ttgtcgaaga aacagcacgt ttattattca gagattatca gtataatgaa atgcgtacac
    33721 cgatttttga acactatgaa gtgatatccc gaagtgtagg ggatacaacg gatatcgttt
    33781 caaaagagat gtatgatttt tatgacaaag gggaacgcca tgtgacgctt cgaccagaag
    33841 gtacagcacc aatcgttcgt tcttttgttg agcataaatt gttcggccca gagtatgcga
    33901 aaccgttcaa aactttttat atgggaccaa tgtttcgcta cgaacgccct caaaaaggac
    33961 ggttacgtca attccatcaa atcggagtag aagcatttgg atcagaaaac cctgcaactg
    34021 atgtggagag catggtgatg gcacttgatt tcttcaaaca gttggggatc acgcaaatcc
    34081 ggctagtcat caattcatta ggtgataaag aaacgcgtca agcctatcgt caagcattga
    34141 tcgactatct tttacctttt gaatcagagc tgagtgaaga ctctaaacgc cgccttcatg
    34201 aaaatccatt acgcgtattg gacagtaaag acaaacgaga tcaaaaattt gtggcagaag
    34261 ctccatctat cttagattat ctaagcgagc ctgctcaaaa acattttgat accgttgttt
    34321 ccatgctgga tgcattggct gttccttatg aaatcgacag taatatggtt cgcggactag
    34381 attattacac acatacgatt tttgaaatca tgagtgacgc accaaaaatg ggggcacaag
    34441 caacgatttg cgcaggcgga cgttatgaca atctagtgga agaactaggt ggtccttcaa
    34501 cacctggctt cggatttgca atgggtatcg agcgattact cttgactatg gaggcagagg
    34561 aagtagttat ccctgcattt aatgagttgg atgcttacgt ggtcgcactc ggagacgaaa
    34621 caaatatcga agcattgaaa gtggtccaag cgattcgaaa ctttggtttt tcagctgacc
    34681 gcgacttcat gaaccgtaaa gcaaaagcgc aatttaagac agcagataaa gcgaatgcca
    34741 aattagtatt ggtgatcggt gaagatgagc ttgctagtca aacgattaat gtcaaagcaa
    34801 tggcaagccg aaaagaaaaa agttttccat tagaagagat atatgaacat tttgatgaag
    34861 tctatgatga aatgacttta ttcgagtagg aggaaaaaaa taaatggcac aaagaacgat
    34921 ctattgcgga ttagtttccg aacaacaatt agaacaaaca gttacattaa aaggatgggt
    34981 acaaaaaaga cgagaccttg ggggagtaat ctttatcgac ctacgtgatc gtgaaggaat
    35041 cgttcaaatc gtctttaatc ctgaaatcaa caaagaagca tgggaaatcg cggataaatg
    35101 tcgcagtgaa tacgtgatcg aagtgactgg ggaagttaga agacgcggag aaaatgcagt
    35161 aaatcctaaa atggccactg gtgaattcga agtgattgca agtgaaatcg tgattttgaa
    35221 tcaagcaaaa accccgccat ttcaaattga agatgatcaa gcaatcagtg acgaaatacg
    35281 tttgaaatat cgttatttgg atctgcgtcg tccaaaaatg actaaaaaca tcaaattaag
    35341 aaatgcgaca acaaaagcga ttcgtcatta tttagatgat aatgactttc tcgatatcga
    35401 aacaccttac ctagggaaat ctacaccaga aggcgcaaga gactacctag ttccttcacg
    35461 tgtccatgct ggacatttct atgcgttgcc gcaatctcct caattgttta aacaattgct
    35521 gatgaatgct ggattcgacc gttattatca aatcgttcgt tgtttccgcg atgaagattt
    35581 acgtggagac cgtcagcctg aatttacgca agtagatatt gaaacaacgt tcttaacagc
    35641 agaagaaatc caaacttaca cagagggatt gattgcgaag gtaatgaaag aagttcgcgg
    35701 tatcgaagtc acattgccat ttcctcgtat gacctatgat gaagcaatgg ctcgctacgg
    35761 cagcgacaaa ccggatacac gttttgatat ggaattgatc gatctaagcg atacagtcaa
    35821 agaagtagaa ttcaaagtct tccaaatggc tcttgaaaac ggcggtgtcg tgaaagcatt
    35881 gaatgctaaa ggcgctgcag atcgctactc acgaaaagac atggatcagt taggtcaata
    35941 tgtaggacaa ttcggagcta aggggcttgc atggctgaaa gtagaagaag acggattgaa
    36001 aggtccaatt gctaaattca tgggagaagc aacagaagca atcattaaag caacagatgc
    36061 aaaaccaggt gacttgctga tgtttggcgc agataaaagt gaaatcgtcg ctgctgcatt
    36121 aggtgctatc cggacacgtt taggaaaaga attaggacta attgatgaat cgaaattcaa
    36181 tttcttatgg gtaactgatt ggccacaatt tgaattttca gaagaagaag gtcgatatgt
    36241 ctctgctcac catccattca cgatgccaaa agaagaagat atcccgttat tggctgaaga
    36301 ccctgcaaaa gtctatgcag aagcttatga tatcgtcttg aacggttatg aattaggcgg
    36361 aggttctatc cgtattcata ctcgtgagct tcaagaaaaa gtactagaaa ctctaggctt
    36421 tacaaaagaa caaatggaag aacaatttgg tttcttgtta acagctttag attacggatt
    36481 ccctccacat ggcggtattg cattaggatt ggaccgtttt gcaatgttat tagccggaga
    36541 agataatatt cgtgaagtca ttgcctttcc taaaaacgga aaagcagcag atgtgatgag
    36601 tgatgcacca agtatggttt cgcctttaca attgttcgaa ttgaatattg atgtaacgaa
    36661 tatagaagaa tagccgataa aaaggtaaaa aggatttgtc gaatacgctt cttttgacaa
    36721 ccttgattta attgaaaaac actttataat gaagccataa catataaagc ggggcttttc
    36781 catgaaaaaa acattcgaaa attgggctta tcatgattac acgaccaagc tttcggtatc
    36841 tatcgtttat gcgttgatcg catcagtcgc attgaatttc ttttatcagc caggacatat
    36901 ttattcaagt ggtattactg gtctagctca gattttgacg actttgtcaa aatcactgat
    36961 aggattcgaa gtaccggtat caacaacttt gtatttactg aatatacctt tgtttgtatt
    37021 ggcgtggtta aaaatcggga agaagtttac aattttcact tttatcacag tcactttgac
    37081 ctccctttgt atccatattt taccggaaac agtcctatct accgatccaa tcatctgtgc
    37141 gttgtttggg ggggctgtga tgggactagg tattgggcta gcgttgagaa acggcctatc
    37201 atcgggcggt ctagatatcg tgagtatcac gattcgtaaa aaaacaggaa agtcgattgg
    37261 ttctatttcg atctacttca atgcattgat cgtttttgtt gccggaattc tatttggctg
    37321 gcaatatatg ttttacagta cgttgtctat tttcgtcagt ggaaaagtaa ctgatgtcgt
    37381 ttatactaaa caaaagaaga tgcaggtaat gatcgtaacg aaacatccag aaaaagtagt
    37441 aaatgaaatc caaaactgta tgcgtagagg gatcacgatc atcaatgaag cagaaggtgc
    37501 ttataagcat gataagcaga cagtattgat gacgatcgtt acacgatacg aactgcctct
    37561 acttagagaa gcaatgaaag cttctgatcc acaagctttt gtaagtattg cagaaaacgt
    37621 ccagatactt ggtcgtttct atgaggatgg catttagatc gagtttgaca aaaattcatg
    37681 aaatactgct gtagacagac gttcgtttgt ctgcagtttt tttctaccat agtagaatca
    37741 gcaaagaaaa actgcttttt tatttgaaat tttcttaaag ttgaaaaaaa gcatgtttat
    37801 ttttttaaat ttatgggatg atagaaaaaa atagtagaga aaggacacga ttctacttgc
    37861 tattttcact taatacccgt atattacgaa tgaaaaaagt ttatgaaaag ttggaatatt
    37921 gaatggaaaa acaaaaaagg ttaaaaaaca gccgctacat cactgggttt gatgggctcc
    37981 gttcattagc tgtggtgggt gttatcttct atcaccttct gccaactagt atgaaaggcg
    38041 gctatttggg cgttccgatt tttttcgtcg tatctggtta tttgatcacg gatctgctgc
    38101 ggcaggaatg ggaacaaaat ggaaaaatca atatttggca attttatgtc agaagaatga
    38161 agagactcta tccggcactt gtttttatgc ttgtgacagc ttcggcttat atcactttgt
    38221 ttcagcgagg gctgttaaac aatcttcgag gagttgtggt cagcagctta ctgtatgtca
    38281 acaactggtg gcaaatcaat aacggtttat cttatttcga tcgatttgct aatgagtcac
    38341 cgtttacgca tatctggtct ttagcagtag aaggtcaaaa ctatttggtt tggccgatct
    38401 tgtttgtatt gttgatggta tttgttcgta aaagaaaatg gatcttttat actgtgttag
    38461 gtacatcctt cctctccgcc atcttgatga tgatcttgta tacaccagga ggcgatccaa
    38521 cccgtgttta ttacggtacc gacactcgtc tgttttctat atggttggga agtgcgcttg
    38581 cgtttgtttg gccaagtacg cgtttaaaga aaaacatccc gaaacaagcg aaaaatattc
    38641 tgaatatcgc tggaggaatc tcgcttttgg cactgattct ttgcttcttc tttttagatg
    38701 atcattacaa tttcatctat tacggaggaa tgctcctagt gagtctgtta tgcgtagtgc
    38761 tagttgcagt gacagcacac cccggagcaa gtttgaatcg atggctgaca aatccagtat
    38821 ttacttggat tggcaaacga agctatggca tttatctgta tcagtttcca gttatgattt
    38881 tttatgaagc aaaggtgact gatattgccg atcatgtttt gttgcatact ttagtggaaa
    38941 tcacattgat tctaatcatc agtgaacttt cttatcgata tgtggagcgt ccgctagcac
    39001 gttttgatta tggtcgtaca ttccaagtga tgaaaggatg gttcagcaaa ccgatcatca
    39061 gtaagagcaa accatgggtt gttccaggca cattacttgt agcaattgca ctcgttggtt
    39121 tcgttacggc accgaaaaac gctgtaacag ccgatcaaaa acaactgcag gcaaaaatcg
    39181 ctgaaagtaa aaaattagca gaagaaacca gaaacaattc agaaaacacc actagttcgg
    39241 ttgaccaagc gatccttgat aagtacgagt tgtccaaggc acaagggaat aaggcacaaa
    39301 aattagaact aacggcattt ggtgattcag ttatgttgga tgctgcaacg gatctacaag
    39361 aactttatcc aaaagtagtg gtagatggag atgtcggacg acaattatat gccagtgtac
    39421 cgtacattga aaaattaaag gaacaagatt tgttgaaaga cactgttctg attggactag
    39481 gaacgaatgg ctcattctcg gaagctcagt ttgatagtgt gatggatgca atcggtgatc
    39541 gaaaagtata ctgggtcaat gtccgagtac cgacgaagcg ctggcaaaat gacgtgaatg
    39601 cgatgcttga acagatgacg aaaaaatacg acaatatgac gctgattgat tggtacagct
    39661 acagtaatga ccatagtgac tggttttatg atgatcaagt ccatccaaat cctgaaggga
    39721 tgacacatta cattcactta gtcagcgaag ccttgctagg aggaagttca agtacgaaca
    39781 gcagtgagac aaacgtgaca gaatgatagc tacaaaaaac ttcaggcaaa aacatttgtt
    39841 tttgcctgaa gtttttttat atttaccgtg tttttaacat tcatatagat tcaggaagca
    39901 aaacggttga gtgtgtctgt aaataacgtt ctttttgttt tcgggtgagt tttttgaatg
    39961 cagcttctgc ttttgttgct tcgcttcttg aagaaaatac ttctgcatgg atcatcttaa
    40021 caggtcttct tttttgaaga cgagtatatt tagcaccagt accattgtta tgttcctcta
    40081 atcgtctaga aagatcggtt gtatatccac cataaaatgt ctgatcttta cagaataata
    40141 cgtaaaaata atgttcattt gccatagagc atctcacgaa tctctgcagt gtattctcca
    40201 tccatattgt aagtgatcaa tggtggagcg actttgaatc cgtctaattt gccttctttg
    40261 attccttcaa ctaacaaaat atttgcctct tttccaggtt ttgggtaaac aaattggatc
    40321 cttttaggtg caattcgata gtcttccatg acctgaatga tttctaagaa ccgatcagga
    40381 cgatggacca tagtaaagcg tccatttgtt tttagtagct tacttgaaat atcgatcact
    40441 tcttctaacg tcgtatgaat ctcatgacgg gcgattgcca ggtgtttatt agggttcgtt
    40501 ttatttgttg gcagcccttt gaaataaggt ggattacaga cgactaagtc acaagaatta
    40561 tgtcgaacga cagataagct gtctttcaga tcgatggtgt ggacagtcat ttgttcattt
    40621 aaatggttaa gcgcgatact acgtcttgcc atgtctgcta gacgaggttg aagttcgata
    40681 gcgtcgatag ccgcctgggt tcgtttactt aaaaaaagtc caactgcgcc atttcctgca
    40741 caaaggtcaa caatcttccc ttttttaggt acacgggaaa agtgagcaag cagcacagca
    40801 tctaaagaaa aagaaaacac tttggaactt tggataatct tgatatcttc tgcgtataat
    40861 tggtcgattc gttcgtcttc gtgaagcatg gattccctcc aaaatgaaaa aaatagtcgt
    40921 ctgtattata ctatttttat taaaaagaac caaggtggct tgtcaaacct ctatttttta
    40981 tgcatactat gaccataaga agggaaagga agagactatg ttttatcgat ttatgcgcgg
    41041 ggtagttcga gtactattag ccttagttaa tggaaatgcc cgctatgaga ataaagaagt
    41101 tttaccgcaa gatgagaatt acatactagt tgctccccat cgtacttggt gggacccatt
    41161 atatctggcg gtagctgcag cacccaagga attcagcttt atggcaaaag aagaactgtt
    41221 caaaaatcca gttctgcgat ttatattaac tcgatccaat gcttttccag ttaaaagaga
    41281 aaatccagga ccaagcgtca ttaaaacacc tgtaaagatt ttgaaatcga caaatttgag
    41341 tttgatcatg tttcccagtg ggacaagaca tgctacagaa cttaaaggcg gaatggcatt
    41401 gatttccaga atgtctaaag cgaaaatcgt gccagctgta tatcaaggtc cgttgacact
    41461 tggggatctg ttcaagcgtc agagagtgac agtgcgtttc ggcgaaccaa tcgacttatc
    41521 agatatcaag aaaacggata aagaaggtat ggcggagatc gaacgacgaa cacaagcagc
    41581 ttttgacaaa ttggacaaag aagtgaaccc tgatttcaaa tatgaagtca aaaaaaaata
    41641 aaatgataag tttatgcgtg ggcggtcttt tgaatctact gtcctgttca gttcagactt
    41701 tttcaatgaa aacgagggag agacgtcaag tctgccctct ttttgcgttc caatatgtaa
    41761 aatagaagac aggtgtcatg taatggatca aagtgttata ataattagaa ggacattttt
    41821 tgaggtgatt gaatgcgttt tttacataca gccgattggc atatcggaaa aaaactacaa
    41881 ggttatgact tgattgaaga ccagcgtcat gtactatctt ctatactaga tatcgcgaaa
    41941 gaagaaaaag tagaggcaat cgtgattgcc ggagacttat atgataggag tgtcccagct
    42001 gttgatgccg tagaattata caatgaattg atggtcgatt ggaatttaaa ggaaaagatc
    42061 ccgatttttg ctatatcagg aaatcacgat agcagtacga gactttcagc gggtacgcca
    42121 tggttcagtt attcgaacta ttttttgcga acacagctag cagaagcatt tgaaccagtc
    42181 gagtttaaaa acgtacaatt tttctttctt ccttactttg aaccaattgc agcgagatta
    42241 tattttgaag atgacaatat ccgaacgatc aagcaagcgg tagccaaggt gatagataaa
    42301 atgcaggagc tatttgattc agaaaaaaaa caagtattga ttagccattt ttttgtaatg
    42361 ggaagtgtaa aatctgattc ggaaacgaaa atcgaagtag gcgggttgga ttcgatccct
    42421 ggtgaactac ttacaccgtt tgattatgta gcactaggac atttgcataa tcaagctgct
    42481 ttgagacagg ataatgctcg ctatagcggt tccccattga aattctcttt atctgagatc
    42541 aaccaaaaaa aaggcgtatg gatcgttgac ctgacaacag gcctggagtt gaattttagg
    42601 gagatcgaac ccctgcatga tattgaagaa ataacagcga gtttcaaaga attgctggat
    42661 cctgcctttt atcagtccgt caatcgggag aattacatct atatctatct cactgataga
    42721 acgatcatcc cgaatatgat gaaccagctt cggcaaatat atcctcgtat tttggggatt
    42781 gaacgggcaa acggtcgagc agaacaaaca aagcgcaaac gaaaagtact agaaatgaaa
    42841 gatcctcaaa aattagcaga agcatttttt agtgaaatga cggaagagca gctaactgca
    42901 aaccaagaaa aatggctcgg agaaacgttg caagaaatca tggaaatgga atgatcaaga
    42961 atgaaaccga aaaaattaca acttaaaaat tttggtcctt ttatcaatga gaccgtagat
    43021 ttttcacgtt tgacagaagc accgcttttt ttgatcagcg ggaagacagg agctggcaaa
    43081 acgacgattt ttgatgggat caccttcgca ctttttggtg aaacttccgg aagacttcgc
    43141 tctggaaaag agatgcgttc tttgtttgct acaccagacg aagaaacaag cgtgactttt
    43201 tcttttgaac atcagcagat gagctatgaa atcgaacgaa aaccagaaca agttttagca
    43261 aaacgaaaag gaaacggaac aaaaaaacag gcagccaaag taacgttgac catttttgat
    43321 gggaaaggaa aagaaataaa acaaatcaca aaacgtacag aagcagatca gttgatcaga
    43381 gaactgatga atctcgatgc gaaacagttt tctcaaatcg ttttactacc ccaaggagaa
    43441 ttccgtaatt ttttggtttc atcaagcagt gataaagaga ctgttttacg tcatttattc
    43501 ggtactcaat ttttccaaca gttcaatgag cgattaaaag aaaaagcaaa acaacagcaa
    43561 caaaaattgg atcatttaga gcaagagctg catcttctgc aaaaaaggtt tgttttagtc
    43621 aaagaagagg aaccagcagc agggtttgaa gccgttttag cccaatggac aaaccatcag
    43681 gcaatactcg aaaaacaaat cacggaagaa aaagaagctc taacggaact tcaggaaaaa
    43741 caaaagcaac tagaaaattc ctactatcgt tttgaaagct tgaaaaaaag ttatgaagaa
    43801 aaaacgatat taataaacaa gcaaaaggaa ttagctgctc aaaaagacga aatagaggaa
    43861 aagaaacgat ggattgggta ttatgaattt acgcaacgat tagtcgaacc aatccagcgc
    43921 ctacaagaaa taaaagttga aaaaatagaa ctgtcacaaa aaagtagtga gcaaacaaag
    43981 aaactagcga tgactacgca gaattatgaa gagtggcaaa aaaatgaagc aaaccgcgta
    44041 cgccgacaaa caaaaatcaa agaagctgct caaaaactgc aaaacaaaca gatgatggtg
    44101 ccaattgtcg aatccttacg tagtaaaaaa caagacgcag aacaattaac aatttcattg
    44161 gataaagaag aattgaatgc cgagaagctg tatcaaaatc tcgctatcta tcaggaaaga
    44221 actcgaattc ttcaagaaaa tttactggat caggaaaaac tgcaagaaga tcgtcttgct
    44281 tttggacagt taaaatatct agatgaaaag cagcaagaaa tagaaaaaca aaaagcaaaa
    44341 gacactgggt cgctcgcaca gataagagaa gaactcaaaa aagcaactga agaaataaca
    44401 aaaaatagtc agctattgaa agaacagaca gaacaagcag aacagctgaa aagccaatgg
    44461 gcaaagcagc aaattgctcg tcttcagctt tttttgcgtc caggggagcc atgtcttgtt
    44521 tgtggatcaa cggaacaccc catacaacaa ttaaaacacg aacagccaaa taaggaagat
    44581 atcttaaaag tagaacaaca actggcagaa gctgagtctg ctgtagaaag aactcagcaa
    44641 atcattgcca aaagtgaagc acatgaaaat aatcttcaac aacaattatc agatttagaa
    44701 caaaaaatca tggcaacgaa tatacaattg gctgaacaag aagaacactt gcaacaggct
    44761 ttgaaaaagg catttcctga gataacagaa caaaaaacaa aaacaactct tgtagtaatc
    44821 gaacaatctt tagatagacg aaaaagcaag atggccaatg aaagaataga gctggaagaa
    44881 ataagacaac aagcagaaaa gctcgaacaa caattagctg tctacaaaca gcagcagaca
    44941 gaaaaaaata atcagctcca gcaactgcaa ggagaaatca aaagtttaga acaacaattg
    45001 caagatacgg atagtaactg tttagaagag gagatacgac gacttgaaaa acagttagaa
    45061 gaagactcga atcaactagc tgttgagcag aaagcaggag tgcaattgtt gcaagatctt
    45121 catacgcttc ggacacagct tgatcttcaa catgaacaaa tagaaaaaat agatgaaaaa
    45181 atagaaaagg aacaagaaag aatagctaaa aagttagaaa gccagtcgta tttttcaaca
    45241 attgaagaaa tctgtgcatt tgcacaacag caagcacaat ataaagaaaa ccaacgtaca
    45301 gttacttctt atcatgaaga gcaattagtg acagctgacc ggctgcgtca attagaaaat
    45361 atccagctaa ctgagacatt cccagataca gaagagatca gccaacagtt agaagaaata
    45421 agacgtatga tcgaaataaa gcaagaaaag atatacggtt tacaagaaca aaaaattagt
    45481 aatcaaaaaa tctacgatga atgtctttcg atttatcaca caagccagac gaaattagat
    45541 gagcttacgc aattacagca gctttcgcaa acattcaacg gagaaaatcc taagaagact
    45601 agtttggaac ggtatgtgct tcaggtttat cttcaagaag tgttacaagt agcaaatgat
    45661 catctccaac gtttaacgaa aaatcgttat caatttgagc tagcggatac cattggcagt
    45721 tatcgaggga aaaccggatt agaaatcaat atctatgatg atcaggcagg tatgtctcga
    45781 agtgcgcata ccctgtctgg cggtgagagt tttattgcag cactttcttt agcattagct
    45841 cttgccgatg tgatccagac acaagctggt ggtgtagcaa tcgatgcatt gtttattgat
    45901 gaaggatttg ggtcattaga tgaagaagct ttggaaatgg caatggaagc tttagaaacg
    45961 atcgaaagtg aaggaagaat gatcggcatc attagtcatg taagagaatt gaaagaacga
    46021 gtattacaac aaataagaat tgatacagaa ggtagcggac aaagcaaaat acgctatcaa
    46081 cttggataaa aagggagatt ggtaactatg aaatggagta ttccagaaaa agtcattgca
    46141 cgtggacgtc aatacttgca agaaggccga gtgttatcgg ttgtccctga ccaagaagaa
    46201 aacgtgtggc atgcagaagt cttgggcagt gaattgtata tggttgattt ggatggtaca
    46261 gcaaaagaga tagattactg ccaatgtccc tattgggagg aacataaata ttgtaagcat
    46321 acagttgctg tggaacttta tttacggtcg aaaaatctat ctcgtatcat gaaaaaagac
    46381 cagccaatgc tagaaccggt aaaagcaaca agtgagggag aaatcattgc aaaaggtttt
    46441 tctcgtctaa aaacacaaaa caatcctcaa gcggtgcaac ctttgattat tgaataccaa
    46501 gtagaaacga ttgagactaa tcagtatcac ccagagcttt ctattttggc actttacttg
    46561 cgtatcggtt cattggcatc accgaaaaaa acatatatcg taaaaaacat ctatgatttt
    46621 ttacaagctt acacagaaaa agaaacttac actgttaata aacagtacca gtttcgtcta
    46681 gataaacggg cgtttcaaaa agaagatcaa aaaatactca cgcatcttgc aggaagtgct
    46741 cagacgcatc aattattagg tacgaacggc ttacaagtca aaggaaaact agataaaaga
    46801 tatctgcttc tcccagtaga acaagcccgc ttcctgttag agcagatgaa tcagactaca
    46861 cgtttgcatc tgcagataaa tgaacaaaac gatcaaggaa tacaattttc tgatcaaaac
    46921 aaacctttat cattcaaagt ggaaaaattt ggagaagatt atttgctgca agcattgaat
    46981 gattttgatt ttttctttat gcattatcag tggggagtta tccaagggac attttattct
    47041 ctgacgaaat atcaacaaac ggtttatcaa acttggaaac aattgattcg ccgattggag
    47101 aaaccagaag tagtgttcaa gaaaaaagaa ttaccgtctt tgtttaaaga gattatccct
    47161 ctgctgtcag agatcggcga cgtagctatt gcccctgcag tagaagagga agtagcagat
    47221 atccccgtcg aatttatttt ctttttccga aaagcaaaag gaaaaatcca agctcgagtc
    47281 gatttcgtct atggtgaagt catctattcg actgacccga agcatgaggt ctcgacagaa
    47341 caggcttttc aggtgttacg tgacacagta gaagagaaac gagtacttga tctattcaga
    47401 atgtatcgct accagaaaaa tgaaactggt tatgaacgtc cattgccaag cggggaagaa
    47461 ctatatgcct ttttccgcac agaactatct gtcttccgtc agtttggtga agtcagactt
    47521 ggaagaaaat tgcgagagtt gtatttagat gcgcataaac atcaaccgaa aatcgaagta
    47581 accgagaccg gttcatggct cgatattcga ttcgatgtga cagggataga ggagcaagaa
    47641 atcgatcatg tcctgcaaag cttgctgaga aatgatgctt tttatacatt agaaaatggt
    47701 caggttcttt cttttgattc agaagaattt caacaaacaa gccaagtgtt gcaacaatta
    47761 cgggaatcga tacgtacaga agaaggaacg atccatgtac ctaaaaatca aggattgatc
    47821 attcaaaatc aattggaaaa aagcaatgct acttttagtg aatcgttcca aacgatggta
    47881 caagatctca ttcatccaga aagataccag gcacagcttc ccaaaggatt gaatgcaacg
    47941 atgagagatt atcaaaagca aggttttcga tggttgaaga tgttaggtca ttatcagttt
    48001 ggcggtattt tagccgatga aatgggactt gggaaaacac tacagacaat tgccttttta
    48061 ctttcggaaa aagaagaacg gaaaagcttc tctgctttga tcgtagcgcc tgcgagctta
    48121 atttacaact ggcaggcaga ggtgagaaaa ttcgcccctt ctttaagtat ccaagtaatc
    48181 aatgggaata aaaaagaacg tgaagaatta ctggctaaag acacagatat ccgagtgact
    48241 tcttatgcca gcttaagaca ggatctagct aattatcagt cgcaaaaaat tgattatttg
    48301 attcttgatg aggcacaaat ggtcaaaaat agcagtacga aaactgcaca agcattgaga
    48361 gaattagctg tacctcagcg gtttgcactg agtgggacac caatcgagaa taatttagaa
    48421 gaattgtggt cattgtttgc aacgattatg ccaggatttt tcccgactaa aacaaaattc
    48481 agggaacttg ccgcagaaga aattgcgcaa atgattcgac cgtttatttt acggcgagat
    48541 aaacaaactg tgttgaaaga cttgccggaa aaaacagaga tgaatctata ttctgcactt
    48601 acagaagaac aaaaaactgt ttatttagct tacttgcgtc aaatgcgtga agaaatcaca
    48661 tcaatggatt ctgaagcatt caagaaaaac cggatcggga tacttgctgg tctgacaaga
    48721 ctgcgacaaa tttgctgcga tcctcgtctg tttattgaag attaccaagg cggttccggc
    48781 aagctcgaac aagtaaaaga tctactggta gctgccaaag agaacaaacg aagagttttg
    48841 ctgttttctc aatttacagg gatgctgact atcttgcaag aagagttagc cgagctaggg
    48901 atctcgacat tttatttaag aggcagtacg aaaccacaag atcggttaag tatggtagac
    48961 gcatttaatg ctggcgaaaa agatgttttt ctgatttcct taaaggctgg gggaactgga
    49021 ttgaatctca cgggagcaga tacggtcatc ttgtatgacc tttggtggaa tcctgccatt
    49081 gaagaacaag ctgctggacg tgcacatcgg atcggacaaa aaaatgtggt agaagtgtgg
    49141 cgtatgatct ctgaaggaac gattgaggaa cgcatggatt cactccagca agaaaaacgc
    49201 gaactgttcc aaaaagtgat ccaaggtaat gaagaacagt tgacgaaact gactgaagaa
    49261 gacatccgca tgattttaag tgtaggagaa atcgaggagt aagagactcg aaaaagcgtt
    49321 cagtaatggg taagaaagcc tttctaaaga agcgattgtt tatttgattt aaaaaatagc
    49381 aaggaggctg ggattccatt ttatcccagc ttccttgcta tcattttttt atttctattt
    49441 ttaaaggtta tacaaagatt gtcttcattg tctctaacag ctgatcaaaa gaagaaatct
    49501 cgatatcgca aattggagtc tgttcaattt tgcgttctcg atggttgaac caaagcgctt
    49561 gccagttagc actttttgca cctagaacat cattgtcgta attatcgcca acgtataagg
    49621 tctcttctgg aagcatatga aattctttct cagccagctg aaaaatctct ttttctggtt
    49681 tttggaaacc tgttgcttga gaaatgagca tattgcggga aggaatccaa ttgttcaatt
    49741 gaagttgctt cagttttttt gtctgatggt cagtcggccc atttgtgata attcctaatg
    49801 ggacattctt ttccttcaag aaatcgaggg tttttttgac ctcctcatgc aagcagatat
    49861 tatcaagttc ttcctcgtaa attttttgaa ataagaggcc atcttcttcc gtaatatgag
    49921 ggtagtctaa atcttttagt gattggctga tccgatgtgc tcgcatatat tctagtgtcc
    49981 aatctcctgc cattactttt gggaagtttt catcactatg atggcggaac cggatatata
    50041 gtggatgcat atctgcatct gaaacaagcg gaacgactct tttgaccgcg tttcgaaatg
    50101 gctgctgctg atcatacatc gtatcatcaa catcaaacac aactgattta atcaaaattt
    50161 tacacgcctt tcggttttca ttgatttttt cacaaatcct tgactattgt aacagaaaaa
    50221 attgtctaaa aaagctgata aataaaggtt tttcttacta tatttctatt tccttgacag
    50281 aataaggttt ttatatttca gtttttttca ctaaaaatcg tgaaacacgt caataagttt
    50341 gatgaacaaa ttaatcatta tggtagaagt cgattgattt attttaatgc aatctctgtg
    50401 gaattttcga aagatttat
//
LOCUS       DAPCMT010000006        47134 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPCMT010000006 DAPCMT010000000
VERSION     DAPCMT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 47134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.006"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            252..2396
                     /locus_tag="RD743_000259"
     CDS             252..2396
                     /locus_tag="RD743_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDU0562120.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2448..3488)
                     /locus_tag="RD743_000260"
     CDS             complement(2448..3488)
                     /locus_tag="RD743_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDU0562121.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(3765..5567)
                     /locus_tag="RD743_000261"
     CDS             complement(3765..5567)
                     /locus_tag="RD743_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562122.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(5581..6333)
                     /locus_tag="RD743_000262"
     CDS             complement(5581..6333)
                     /locus_tag="RD743_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562123.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(6342..6704)
                     /locus_tag="RD743_000263"
     CDS             complement(6342..6704)
                     /locus_tag="RD743_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDU0562124.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(6880..7752)
                     /locus_tag="RD743_000264"
     CDS             complement(6880..7752)
                     /locus_tag="RD743_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDU0562125.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(7778..8290)
                     /locus_tag="RD743_000265"
     CDS             complement(7778..8290)
                     /locus_tag="RD743_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDU0562126.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(8428..9219)
                     /locus_tag="RD743_000266"
     CDS             complement(8428..9219)
                     /locus_tag="RD743_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDU0562127.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(9274..9882)
                     /locus_tag="RD743_000267"
     CDS             complement(9274..9882)
                     /locus_tag="RD743_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDU0562128.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(9875..10192)
                     /locus_tag="RD743_000268"
     CDS             complement(9875..10192)
                     /locus_tag="RD743_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDU0562129.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(10348..11076)
                     /locus_tag="RD743_000269"
     CDS             complement(10348..11076)
                     /locus_tag="RD743_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDU0562130.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            11235..12116
                     /locus_tag="RD743_000270"
     CDS             11235..12116
                     /locus_tag="RD743_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0562131.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(12353..13396)
                     /locus_tag="RD743_000271"
     CDS             complement(12353..13396)
                     /locus_tag="RD743_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0562132.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(13399..14067)
                     /locus_tag="RD743_000272"
     CDS             complement(13399..14067)
                     /locus_tag="RD743_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0562133.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            14209..16545
                     /locus_tag="RD743_000273"
     CDS             14209..16545
                     /locus_tag="RD743_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0562134.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(16660..16971)
                     /locus_tag="RD743_000274"
     CDS             complement(16660..16971)
                     /locus_tag="RD743_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562135.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            17152..18627
                     /locus_tag="RD743_000275"
     CDS             17152..18627
                     /locus_tag="RD743_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDU0562136.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            18674..19258
                     /locus_tag="RD743_000276"
     CDS             18674..19258
                     /locus_tag="RD743_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDU0562137.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            19260..19679
                     /locus_tag="RD743_000277"
     CDS             19260..19679
                     /locus_tag="RD743_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDU0562138.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(19960..20244)
                     /locus_tag="RD743_000278"
     CDS             complement(19960..20244)
                     /locus_tag="RD743_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDU0562139.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(20596..21306)
                     /locus_tag="RD743_000279"
     CDS             complement(20596..21306)
                     /locus_tag="RD743_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDU0562140.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(21303..22775)
                     /locus_tag="RD743_000280"
     CDS             complement(21303..22775)
                     /locus_tag="RD743_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDU0562141.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            22913..23386
                     /locus_tag="RD743_000281"
     CDS             22913..23386
                     /locus_tag="RD743_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDU0562142.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(23539..24090)
                     /locus_tag="RD743_000282"
     CDS             complement(23539..24090)
                     /locus_tag="RD743_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDU0562143.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            24195..24725
                     /locus_tag="RD743_000283"
     CDS             24195..24725
                     /locus_tag="RD743_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDU0562144.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(25208..25651)
                     /locus_tag="RD743_000284"
     CDS             complement(25208..25651)
                     /locus_tag="RD743_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0562145.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(25667..26827)
                     /locus_tag="RD743_000285"
     CDS             complement(25667..26827)
                     /locus_tag="RD743_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDU0562146.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            27334..29034
                     /locus_tag="RD743_000286"
     CDS             27334..29034
                     /locus_tag="RD743_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562147.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(29136..29969)
                     /locus_tag="RD743_000287"
     CDS             complement(29136..29969)
                     /locus_tag="RD743_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDU0562148.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(30273..32438)
                     /locus_tag="RD743_000288"
     CDS             complement(30273..32438)
                     /locus_tag="RD743_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0562149.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            32698..34992
                     /locus_tag="RD743_000289"
     CDS             32698..34992
                     /locus_tag="RD743_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDU0562150.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            34985..35650
                     /gene="pgmB"
                     /locus_tag="RD743_000290"
     CDS             34985..35650
                     /gene="pgmB"
                     /locus_tag="RD743_000290"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDU0562151.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            35643..36650
                     /locus_tag="RD743_000291"
     CDS             35643..36650
                     /locus_tag="RD743_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDU0562152.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            36672..37691
                     /locus_tag="RD743_000292"
     CDS             36672..37691
                     /locus_tag="RD743_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0562153.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(37972..39339)
                     /gene="nhaC"
                     /locus_tag="RD743_000293"
     CDS             complement(37972..39339)
                     /gene="nhaC"
                     /locus_tag="RD743_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDU0562154.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(39449..40870)
                     /locus_tag="RD743_000294"
     CDS             complement(39449..40870)
                     /locus_tag="RD743_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0562155.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(41113..42990)
                     /gene="tdc"
                     /locus_tag="RD743_000295"
     CDS             complement(41113..42990)
                     /gene="tdc"
                     /locus_tag="RD743_000295"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDU0562156.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(43281..44537)
                     /locus_tag="RD743_000296"
     CDS             complement(43281..44537)
                     /locus_tag="RD743_000296"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDU0562157.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(44649..44854)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            45117..46418
                     /locus_tag="RD743_000297"
     CDS             45117..46418
                     /locus_tag="RD743_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDU0562158.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(46487..47086)
                     /locus_tag="RD743_000298"
     CDS             complement(46487..47086)
                     /locus_tag="RD743_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0562159.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        14454 a         9732 c         8369 g        14579 t
ORIGIN      
        1 ataatttaca tatgcataac catttttatc cccacttgtt tatccccaaa caaaggtggt
       61 ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg ctcgttgctt atttacaaat
      121 tgttttattt agaatacaca gacagattta tgaaaagaaa aagcttttcg acacgattat
      181 ctgttccaaa aaatgtctaa tgaatgctga ataagacaga aaatcaatga aaggaaaagg
      241 actagtttat tatgaaaaat aaaaaaacga tacgtttgct agctgctact tggctgattg
      301 ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga taatcaaata gctgatcaaa
      361 cagtcacaac agatacgact acatccagct cagctactag tgaaacaacg gattctttta
      421 cagaagaccc aactggaaca actaactcaa caacaggatc gaccgagcca actgaaattg
      481 atccggctga tccatcagaa aatgaaaagc cagccgagac atattccctt acaattgctc
      541 cttctttgat tggtaagatc caactgactg cttcgacagc ggattcccct gaacagcaac
      601 ctttggatat tacggaaagt ggaacggtag aaggtttatt agcggaaacg aaggtaactt
      661 acactattac gccagctgaa ggtgaacaaa ttgcttcagt cgctgttaat ggtgccaaag
      721 aagtaaatta tgatgcaaag acctttacag taggtacgaa caatttatct attactgcag
      781 aatttaaagc tggaccggat acttcaacag agaaaccaac tgatcctagt gaagaagtcc
      841 ctagtacacc agatccggaa acgccaaaca aaccagatgt gggtaataac aacaataata
      901 atggttcaaa taattcaaac aataataaca ataattcgaa cagcgggaat tcttcgaatc
      961 aaacaaaccc atctggatcg aataacaatg gaaatactca gagagaacca aaccatccaa
     1021 acaatggttc atcgaatggc ggaacgaaca atgcttcatc tcgtccaaat acagcaaatc
     1081 aaagcggtat agagaatcca tcttctgctt cttctgattt tgttgtaaaa tcaccaattg
     1141 atgctgtatt gcctacaaat acaacgaatg tccaacaagc aatcgttcga gaagcattca
     1201 agcatcttgg aaaaccatat gtatggggag caaaaggtcc aaatactttt gactgttctg
     1261 gtttgactta ttacgtatat atgaaagcaa ccggtcatta tatcggtggc tggacaggcg
     1321 aacagcaata tgcaggtacg cagattccag taagtcaggc ccaacctggc gatctcgtct
     1381 tctggggacc ttcttccggt gttactcacc atgtaggtat ctatattggg aacggacaat
     1441 ttatccatgc gcctcaacca ggagataaag ttcgagtaac ttctatttca gatttcacgc
     1501 ctgattttgc tgtccgagta aacttagcag gtttacctgc tgcttcaggt tcgctgacag
     1561 gcggctcatc tattttagat ggattagatg gctcgttcca tttcagtcaa aaccaaacaa
     1621 ctgatcagtt cttgaaaaag attgcagatg atgcacagga aatcgggcaa aaagaaggaa
     1681 tctacgcatc agtcatgatg gctcaagcta tccttgaaag tggatctgga aacagcttat
     1741 tatcaagcga accaaatcat aacctttttg gtatcaaagg atcatataaa ggaagcagcg
     1801 tgaccttcaa tacacttgaa caagatagtt ccggacaaag ctaccaaatc cgcgcacaat
     1861 tccgtaaata tccttcttac aaggaaagtt tggaagacta tgccgacttg atcaaaaatg
     1921 gactgactgg caatcctgat ttctacaaac caacttggaa gtcggaaaca aaagactaca
     1981 aagaagcaac caaatatttg gaaggacgtt atgctacaga tcgccaatat tcacaaaaat
     2041 tgaacgcgat catcgaagcg tacgacctga caaaatatga tgaaccgaaa aaagaagcag
     2101 caacaactga agaagcaaac gctgaaatcc tcaatatgtc taagcgattc gatgtgccaa
     2161 tcaaatggac aacagatggc gtgccaatca caggattaga attttcaacg ataaaacgcg
     2221 tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg ggatttgtgg aacaatttta
     2281 cggcaagaca gataccagaa acaactactc gtacagttaa agctgcaact gttcctgtac
     2341 taaccttgtt aagtttagac aaatcattct ggcttgatct gcctttaaag aaatagaata
     2401 aattaaaaag gggatgtgac actgtcacac tcccttttta atttattcta tttctttata
     2461 tttattttgt attccgttac aaactcgcgt acttggttag gttccagagt gacatctcca
     2521 aaactttcaa gtcgctccgc tcctggcagt tcttgagtct caaatgtgat accgccatga
     2581 tttactaatt tattcccgcg catttccggt ccatcttctc caaaatttgc tgtaaaaatc
     2641 acgattcctg aagcatctgt ttttacttgg atcgatactt tttcatctgg gcttgtcaat
     2701 tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa atggatgatc gatgccgccc
     2761 actatcattg attggcgaaa atcactggca aagacctcac ctaatttttt aggacgttgg
     2821 aagtcaaatg ctgtgccact tactgaaact acttctcctg taggcgttgt atctttattg
     2881 agcgtagcaa ataatcggct attcaaccaa agtatatgtt cgtcgattga ctgtgctgga
     2941 tcaccggtta aattgaaata gacatgattt gtaggattga atactgtttt gcggtcactg
     3001 atcccttgga ccgtcactcg ccatgtatca tcgttcgtca gcgtataccg tacttctaca
     3061 tctaaagttc caggaaatcc gtttcccata tctgggctag tctgatagaa aatgaccgat
     3121 gcttcgtttt caccattgat gactgtataa tgccactgct tggcttcaaa gctaggtgca
     3181 ccaccatgta aacaatgacc agtgtccggg tctgttttta cttcgaacgt ttcatcatct
     3241 aatttgaatg tgccattcgt aatccgtcca gccatacgtc cgattgaagc accgatataa
     3301 gggtcttttt ctagatattc ttttgcttca tcaaaaccta agatcaactc tcgtttttca
     3361 ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa gatcactgac agacaaagat
     3421 gtcccatttt gattcgtgat cgtaatcaag tgatagtttt cgccaaaatt ttgttcattg
     3481 atttccatgt tccttgcacc ctcttttctt tcatttgcac tgattccagt atacatggaa
     3541 accgttttct taacagctta ttccgtgagt aacgagaaaa aatttactat attctaataa
     3601 aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca acctcttttt ccgaacaaac
     3661 ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgaggagct
     3721 atttttgctt ggctgttctt attacttgat gcaggacagc tttttcacaa tctctttttc
     3781 aatacgattc cagtatataa ccatgtcgtc agtacatagt agatgaaata caatacgaag
     3841 aagatcgtaa acggaaccca aatatctttg tatggatttt gcatgaatag accaaacatt
     3901 tgtaatccga ataagacatg gactacacct aagattcctg gtgctaagaa taacacgcct
     3961 acttctctac ggatcgattg tttaagcaac ccgcgtcgtg ttcctatctt ttcaagcatt
     4021 tcataacgag caatgtcact cttgcttcct gacaagatct tgaacatcaa acagctagca
     4081 agcatggtca agaaagctaa acctaggaag aatcccataa attcaaaacc agagaataga
     4141 ccattataca cttcatacat ttcatatttc tggttgctgt actctacttt ttgcaaagat
     4201 ggattgttca ttttattttg agcaacaagt gtttcgattg gtttcaggtt tgcttggaag
     4261 tcttttacct gaactagctg taaagtggat tgtggcaagt tcagttgatc aaattctgat
     4321 tgactgacta ccttgatttc tttttcttgc tgttccggca attcatagct tcgaagttcc
     4381 tcttgtacag tcagttcatt cattttttta gcaggaactt tttctttcgt gatcttcggc
     4441 ggtgttccat cttcatgttt tatcatgatc aatggttgag ccgcaaattc acttgcatta
     4501 tagtaaatcg ttcccgcatc ttctttttga gtataagaag cagaaagtgt tggtgaaagt
     4561 tctttgattt tttgctgatc ttctgcccga ggattattca tcactagatc atatccagaa
     4621 ttgttttctg tcaagtctgc aatttcattt ttaaaaccta atcctaccgt caaagcgccc
     4681 agtgccaagg caaacagcat agcgaccatt gagagcattt gtgtatattc acgaatacga
     4741 aagtttaact gtgacaatgt gaaattgttc aattttttca atgaaatctg ttctgatttc
     4801 tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa tgaaataagt tcctaatatg
     4861 atcgtaacta aggctacccc tataccaaag acttgatagg tccccacgtg tttcatcata
     4921 tagtaaccgc agcctagaaa agcaattccc agtactactt caaggaaaaa caagaattta
     4981 tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca atgctaaaat cggttttttg
     5041 acaatcgaaa aagcattgac aacagcagct aataaaaaca aaacagcaaa aaacaaaaca
     5101 gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg aaatattgag atctaactga
     5161 tccactaata atcggtggac gatacttgtc aatcctacac cgacagctga acccactaaa
     5221 gtagctgcga tccctactaa gaatgtttca gaaaaaatca tctgtgcgat ttttctgcct
     5281 tttgctccca gcatcataaa cattgcatag tctttttgcc gcatcgtcat caaaaaggaa
     5341 tttgcatata aaatataaac gaatgtgatg atccctagta aaacagaacc gaatcggaaa
     5401 atgataactg tagccgcaac gatcgtatta cttttcaaaa aagcttcgtt gcttgccatt
     5461 gattcaaaca tgtaaaaaat agcagatgcc ataactaatc ctgagaataa caccaagtag
     5521 tctcttaaac ggctcttgat cccagtaagt gataatttcc aaagcatagt caatctcccc
     5581 ctactgttcc aagtttccaa gaatagatag tatttctcga tagaaggaat cacggctttg
     5641 atctccacgt ttcacttctt gatggatacc gccatccttg atgaataaaa tacgttggca
     5701 ataacttgca gaaaacggat catgcgtcac aagaagaatc gatacatgat cttttgtatt
     5761 caattcgtcc atcgtatcta aaagatcccg ggcacttttg gaatccagcg cacctgtagg
     5821 ctcatcccct aatagaatcg ttggttgtgt gatcaatgca cgtgcggcgg cgacccgttg
     5881 tttttgtcca ccggaaatct cagaaggata gctttccaaa atatgagaga tggataaacg
     5941 ttctgcaatt ttttggacac gttgcttgat ttcttttggt ctgacgcctt gtaaagaaag
     6001 aggaacagcg atattttctt gtgctgtcaa gctttctaat aaattgaaat cttggaaaat
     6061 aaagccaatc tcattcgcac gaaaatctgc tagttgattt ccttttaatg tggtcacatc
     6121 tttttgattg attcggatat gcccatctgt tggtttatct aatgtggata aaatattcaa
     6181 aagtgtcgac ttaccagaac cagaagcgcc catgatccca ataaattcgc ctttttctac
     6241 atcgaagctg atgccgctta atgcttgcgt ctttttctca ttgttttttc cgtatacttt
     6301 tgttacattc tgtacttcga ctattttgtt catttctttc cctacccttt aattagtttt
     6361 tccagtgctg ttttcggcac gtcttttttt tcgacttctt catacgaagt gacaccttta
     6421 tttttgttcc aggtgatttt taaatatgct cctttacgaa gcggtcgagt caattgtccc
     6481 ttgaagtcca gttctttttc cttaccctct ttcgtatagc caggcaggat atatctatag
     6541 ttgactgttt cttgtccaga gtctgatttt gaaactgttt ttgtaccatc tgtcgtaatt
     6601 tgtacatagt attcttctcc acccataact acgtaatctg cgattttcac ccctgcaagt
     6661 accacaacga tgaaaacaag tagaccgatc agaagttttt tcactttttc cccttctctc
     6721 ttactttata tactgattat agaaaaaagc gaacaattct gccttcgaat caactatcat
     6781 tcttctttca attttgtcat tttctttaaa acaactaaaa aaagagttca agacaataat
     6841 ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt cacttacggt ctttcatgtc
     6901 gcctgtattg ataacaggtt gcgttccttt gtctgtaatg attgagacaa gtaaattatt
     6961 gatcaattgg actttccgat catcggtgaa attgatttct tgtccatctt cgatttgctc
     7021 taaggccatt tgagtgattg tgacagctcc ttcaacaatg gtttgtcgag cagacaaaat
     7081 ggcttttgct tgttgacgtt ggagcatggc actagcgatt tctgttgcat aggctaaatg
     7141 gttcaagcgt gtttcgatta cttcgacacc tgctactgct aaacgctctt gcagctcttt
     7201 ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc gtcaaatcat tctcattgaa
     7261 tgtatcatac gggtattggc tagcgatatg ccgaatggct gtctcacttt ggatttctac
     7321 aaaatcttga taatacgcta catcgaataa tgcctttgct gtatcaacga ctttaaagac
     7381 tactactgct gagatttcga tggggtttcc atccaaatca ttgactttta aaacagaact
     7441 attgaaattc cgaactttca acgaaacagt cattttttgt gccaaaggta tcgtaaggaa
     7501 aaagccattt tctcgaatcg tacctaaata ttgtccaaaa aacaaaatta cttttgcttg
     7561 attaggactg actacagttg ccgaacttaa caacaataac gtgatcaacc aaagaaaaat
     7621 acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct aaatagaaga aaaacaatcc
     7681 aactaaagct aatacgacca atccgacaat ccctgcataa ccattgatat aaaatgctct
     7741 tttttctctc atgctacttc ctgcgctcct ttatccttca ataaatatct aatgttactt
     7801 ttttaatttt ttttcgaata cgcgccatta caccttctag ttctaactct ttgtcttcag
     7861 tgatgtacca accccttgcc tgtgtccctc cggctgcctg cggtaaataa tccaattttg
     7921 ttcgaaccca gtgcttttct tctgtaaaat agtcttctac caagacctct agatccatcc
     7981 ctgctgaaag aggcaaatct tcccgaacag atttaaagat aaatcgttcc ttcttcccat
     8041 catacttcaa tattcctgta tcgataatcg gtaaagaata ataatccagc tctttcttca
     8101 ccgtcaataa atgtttgaaa acttcatggt acacttgctc taattggcga ccttctgaat
     8161 tgttcgggta attaaccatc actttttctt tgtctggatc atagaaggtt tggcttagtt
     8221 gagtgatata agtatgtaat ctttccattt ctgtttttaa cttttctctt tgaccttctt
     8281 ctaccttcat atactctttc accctgtttt ctttttagat attttctttc ttattttact
     8341 atagttatgt gcgttaagcg atgaaaaaca gcaaagattg gaaggatctt tgctgttttt
     8401 tcttaaaata tccagttgag tacaggatca atctgtttca ttgtcctctt gatccgtcgt
     8461 aatattgatc gatgtgtctt tagcagtgat ctttagcttt ttctgtcctt gttctaacaa
     8521 agtatttgtt attggctggt agtctttttc aatataagat tcaccagacg tttggatagt
     8581 atagccactg ttttccatct catgcaaaga taaattgcca ccgtttagga tgacttttcc
     8641 ttctttgctg atcaaactgt cttctagatg gaagttgctg ttttcacctg tgacgatcaa
     8701 gttatccaca ttggcctcat ctaatcgaag tgagcctaat tcatctttcg cagttattgt
     8761 tcctcctgta aattcattga acgataccca gccatcatgt gtcttagtat tcactttttt
     8821 gattgatact tcgttgatat caattgcact attattccct tcgattgtca attcagataa
     8881 ttcatgatct gcagggacaa caatagtgat ttgtgcactt gtgtcttcta aactaatgat
     8941 tgcacctgtt gattttttga ctgcattcac agtcaacacg ccatttttct cctttatttc
     9001 tggcttttct gtcttatcat aagtcgtttt tatctcaaaa tccaatccgc gtatgaccga
     9061 aactgtttga ttttctgcat ggatgacgat tttgtttgca tccttgattt tttttgtctc
     9121 agtaaccata tcgatcaacc gaggaccatt atcccaagtc agactctttt gagcgcctaa
     9181 tgtataagtt gcacctgcta ggaggccacc agtaagcatc agaccaatac cggtaatcaa
     9241 aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg cccgctgaat gcagatctct
     9301 ttttgtaata aaacgtcgtc ccatccattt aacaaagttc atcatgacat aaaaaagccc
     9361 ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg cttcctgtgg cgaacagacc
     9421 gacaccgata aagaataatc cactcagaat cgattgtcca aacacactga ttcctgagat
     9481 gatcatgaat aggccaacac ctaagatact gatacataaa acatagatac taaaaatgag
     9541 cgctagtaaa gcaaccaata gtcccccaat aaccaatata aaccccagtg ctagtggaat
     9601 gaggacagga gaagcaaata aaccgataat gattaaccaa atcaggcgaa tttgtctttt
     9661 tggatttttc actaactcgt tttctgcttg ttcttcttct gaaaagtaac tgatttttaa
     9721 ttttaaggca aactgctttg gtgtaccata ttcatcaatg atctgatcta atgtttttcc
     9781 agaatctaaa aaatattcct cataatactg gatcaaatca aaacgatcgt cttcaggaat
     9841 ttcttgaaga tgatccacaa cttcattcaa atattcattc atttgtgctc tcctccaata
     9901 accggtctaa agaatctcga aaaacaatcc attcatcaat aatttcttct aaatgacgac
     9961 ttcccattgg tgtcaacgag taatatcgac ggtttctgcc ttcaaaaggt tcatcatagg
    10021 tctcaagata tccttctttt ttcaatcttc tcaaaaccgg atatagtgta gattcactga
    10081 cagagagact ttcttgtacg atttttgtta aggcataccc atacatatct tccttgctta
    10141 atgctgctag cacagtacca tcaagaagaa cagttggaat ctggatgtgc attacgtcac
    10201 cttctattct caagtgtaat attttatttc aatctatatt atacaacata taatatagtt
    10261 tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag atttgatcct ttttagtcag
    10321 caaaaaatgg cttctctttg atttttatta taacagtggc ataatccaat ttgtccacat
    10381 ccagctcagt ggcatgataa cgatcattgc aggtatcatg tcagctactg ggaacatttt
    10441 cacgttgatc attctaaaac cggtagcgac catcaagaat ccgccgcaag ctttaaaatc
    10501 taagatcatg tctggtgtag tcaatggata aataaatttt gccaataaga acaaaatata
    10561 aaaaataatg aattggggaa ttgctatcac agaaacgaca taccctaaat tacacgcaaa
    10621 aatagctgct gtgaaaaaat caaggacaga tttagaaatc aaaatcgtac tgtctcctgt
    10681 catcccagaa tctaaacttc catagatccc cgtaccactt gcacaaaata gaacaatgac
    10741 cgtcactaat gtgctgataa attcatcgtg ggtcatacca agcttttcac tagggaagat
    10801 tttagaaata ggtacttgca tcagcgcagc ccccttattt atcccattac ctaaatgaac
    10861 aatcagacca atcgctgttc ctatcactaa agcaaaaata acagccggca tatttttcat
    10921 cggcgcaatg gaatagattc ccatccccat cgaacacaca ccaaaaacca tcgtcagctg
    10981 tgctttaaaa tgttcactta atttatttcc tactaatccg ccaattagtc cgccaagcaa
    11041 aacggaacct acattgatca aaactccagt tggcatcaaa aaactcctct ctttttctac
    11101 gaagcgaaaa aaatcagaaa aatttctgta attattgctt tcgctgcaag aaagattgta
    11161 acagacagag aagattacac caattcattt tttgtgttaa agtattcttt attggaataa
    11221 gtaggaggaa tatgatggat atccataaat tcgaagtttt tcttgattta gccgaaacaa
    11281 tgaatttcac caaaactgct gatcgacaat tcaccaccca aggaaatatt tctaaacaga
    11341 ttctttcgtt agaaacagaa ttagacgtca aattattcga acgcgcccat agaaaaatcg
    11401 aattgaccga agctggaagc ttattgctcc catatgtaaa aaatgtcgta gaacaatacc
    11461 atgctatgca agaagtgctt gaaacctata cgaaagacaa caatctttca ttaaatatct
    11521 taacgatacc gacaatgatc aattataaag gtttttccaa aatcaccgag tttttgaaac
    11581 gccatcctga atttacagtc cagttgaaag aagtggaaag tgtcgaatta acttacaatg
    11641 acgccaatga acatagtgac acgatccact ttgctagaaa tttccaggcc ccaccaagtt
    11701 cgattgagtg gttgcctact gaaacagacg attttgtagc agttctacct aaaaatcatc
    11761 ctttagctag taaaaagaaa ttggatctaa gcgaactgaa aaaggagaac tttttgctcc
    11821 ttggccccac tacgaacttg tatcaacctg tactagatct gtgtcgcgaa gctggatttg
    11881 aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat catcaatatg attgcaaatg
    11941 acatggggat tgctattgtt atggaaaaaa cagtcaagaa tctattaaaa gaaaatactg
    12001 tgattctccc tatttctcca acaacagaga gttatcttgc atttactcgt aaagtcggtg
    12061 agcattcttt ggcttcagat acattttggg agtatttaaa tcaagaagaa aactaagccc
    12121 tacattttct gtccatctat ccacccaaaa aaagagagcg acgcatgact ttcgctctct
    12181 ttcttgcatc atgtccttgt ttcaatgaca cctctaacat ttctcgtagt tcaataagaa
    12241 agcagcgatt tttatgccaa aagaaccgta agaacatttc cctaatcaca aaaaaacgac
    12301 taaaaacccc taaaaacctt caatagaatt tttaggggtt ttattctttt tcctattgtt
    12361 tacttaaccg tatcattgct gtcgttccta ctccttcgat actgctataa tcaatacgtc
    12421 catcatgcag ttctagaatt tttttaacca gtgaaagtcc tagcccattc ccagaaattt
    12481 gacgacttgt atctccttga taaaattttt caaacattcg atctctggtc tcttcattca
    12541 ttcctacacc tgaatcggtc acttccagtc caatctctgt ggctgtctca aatagtttga
    12601 tatgaatctg tccattgatt tgattgtact ttatggcgtt atccatgata ttcagccaca
    12661 cttggtacaa aaactcttca ttgcctgtgt aatttactgc agcaagttcg atatccaatt
    12721 ctagctgttc tttctcccat ttaggctgca aaaaaacgat tacttctctg atttgttcat
    12781 ctaatcgata ctctttcttt tccagttgga tacgctggtt ttccagtttg ttcaatttta
    12841 agatgttctc tgttaattgt gaaagctgcg tgatactttc gataatccga tataaaaata
    12901 tttggcgttc ttcgtcagag agatttgggg cttgtaacaa ttgaacatat ccttggattg
    12961 ttgctagtgg tgttttgaat tcatgggata cactggacac aaagtcattc ctcagtgtct
    13021 cgatactatt gagttcttgg accataacgt tgaaatcttt atacagttgc tgtacttctg
    13081 ctactttctg ctgttcatcc attcgaatcg aaaaatctcc cgttgctacc agactcatat
    13141 ttgtccctag cgcactgatt ggatgcagga tacgccgccc aacaaaaatc gatattcctg
    13201 tacccacgac catactgaac cccgcaaaga cgaataacgg gtataacgtt ggttttccag
    13261 ccgattcagc aggcgagata gaatgtcgag acaataagaa aataaaaccg agaaaacaca
    13321 gcagtgtcac taagatcgaa atgaagacaa tactagcaaa atagatccat aattgtgaaa
    13381 atagcttttt tttcatcttt aaataaccgc cttatagcct agtccccgta ctgtgatgat
    13441 ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt ttgatatgta catcgatcgt
    13501 tcgttcttct gtattcgtat ctaatcccca aatatcatcc atcaactgtt ggcgagtgaa
    13561 gatcttgttg ggataagaca gtaatttata cagaagcaga aattcttttt gaggcagaat
    13621 caacgaatgc tgatcttgta acactgtata agcatctagc tctaattttg tttgtccgat
    13681 tatcaagcta ctgctgtaat tgatctttga acgtcgaagc aatgcttcca cacgtaggat
    13741 catctcattg acatcgatgg gttttgtcat ataatcatct gctcctaatt tgaacccgcg
    13801 tttcttatcc tcaaaatcgg cctttgccgt aatcattaga acaggtgtgt caaatctggt
    13861 gctacgtaat gagtctagca agtcatagcc atccattttc ggcatcatga tatctgtaat
    13921 gattaattct atacgtgtcg cttctagttt tttcagcgca tcctctccat tcaatgcggt
    13981 gatgactgaa aagcccgctc gttccaacac agaacggtaa agcaactgta attgcggatc
    14041 atcttctact actaaaatac gattcattga tctccctctg ttttttgaaa aatttctaga
    14101 ttttttcttg tagttcatct tcagttaaca tttatttcgt atactacgat cattgttctg
    14161 atatacttat tagtataccg aatatttcaa tacgggagaa gggtgattat gcttcaatta
    14221 aaagatataa aaaaatatta taaagtcggc gaaaccacca ccaaagcatt ggatggtgtg
    14281 tctgtcgctt tcagacaaaa agaattcgta gcgattttag gtcctagtgg ttctggtaaa
    14341 acgactatgc tgaatgtgat tggtggacta gataattatg attccggtga tatggtaatc
    14401 aacggaaagt ctactaaaga ctttaaggac agtgactggg atgcctaccg aaacaactct
    14461 atcggtttca tttttcaaag ttacaacttg attggtcatc taggcattat tgaaaacgtt
    14521 gaacttggga tgacattaag tggtgtttct aaagacgaaa aacgaaaacg ggcagaagat
    14581 gcattgcacc gagtcggctt gacagatcat atgcataaaa agccaaatca gttatctggt
    14641 ggacaaatgc agcgagtggc catcgctcga gcactagcca atgacccaga tatccttttg
    14701 tgcgatgaac caaccggagc gttagataca gaaacaagta tccagatcat ggaattgatc
    14761 caagagttat cgaaagaaaa attagtgatc atggtcacac ataatcctga actggcccat
    14821 caatacgcag atcggatcat tgaattttct gatggtaaga tcttaacaga ttcccatcct
    14881 cacatcgaac gaccaaaaga tgatcaattt aacttacgca gaacaaagat gagtttttgg
    14941 acagcgttaa agctttcctt caacaatatc cgaacaaaaa aaggacgtac tttcttgacc
    15001 tcctttgctt ccagtattgg gatcatcgga atcgcgatcg ttctgtccct ttcttctgga
    15061 ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg cgaaatttcc gattactatt
    15121 tctaaggtaa cgactaacca gactcgtgac gatgccggat tgggtgcaag caaagcggat
    15181 tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag aagataaagc acagcacacg
    15241 aataagattg atcaaacata cgtcgattac gtaacagaca tcgatcctaa cttaagtaat
    15301 aatatcggat ttacccgtac tacaggaatc aatcttttac gtgatgtaaa tggcaaagta
    15361 cagcctgtca gcttctcgaa tcaaaatcct gatacggaat ctctttcttt atctagtacg
    15421 atgtcagcaa tgacaggtgt gggagtgtca agtttcccta cacagcttga cactagcaaa
    15481 gaaaactttt taaaagacaa ttattcttta cttgccggaa gttatccagc ctcagcaaca
    15541 gatgtcgtgc tgatcgtaga cggcaataat aatactaata tcaacgcatt gaagaatctt
    15601 ggatttgatg tgaaagaaga cgaaaaactt gattttgatg atatcgttgg tacaacattc
    15661 aaattagtaa acaacaatac ttactataca aaacttccaa ctgggaattt tatccctaat
    15721 acagattatg atgcgatgta tcagaatgct tctgatgaat tgaagatctc ggggatccta
    15781 cgcgtgaaat cttcttctac aatgaatctc ttatctccag gaatcgctta tagcgatcaa
    15841 ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa tcgtcaaagc tcaaagagac
    15901 agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa atgccaaaca aacattgctt
    15961 agttatttag gtggagacag cttgccttca agtatcatga tttatccgaa caattttgaa
    16021 gacaaagaaa agatcttgga ttatctagat gattacaata aaggcaaatc agatgaagat
    16081 aaaatcattt atactgattt agctggaacg atgactgaac tgactggcgg attgatggat
    16141 gctatcactt atgtccttat tgcttttgca gggatctctt tagtaacaag tatgatcatg
    16201 atcagtatca ttacttatac atctgtgatt gaacggacaa aagaaatcgg tgtactaaaa
    16261 gctttaggtg cacgtaaaaa agacatcact cgcgtatttg acgcagaaac gtgtatttta
    16321 ggtatttctt ctgggatatt aggtgtgttt attgcatggc tagctacgtt ccctatcaat
    16381 agcatcttat acaacatgac ggatctaaaa aatgttgctc aactaaatcc tgtccatgcg
    16441 attattctag tgatcgtctc tactatattg acgatgcttg gcggtcatct tcctgcacga
    16501 atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag aataaggttg tgacaaccac
    16561 atttagattt gagtcataag agcgctgaaa tagtagaaaa cagcaagaaa cgaggaagta
    16621 atatgttcct tgtttcgcgc tgtttttttt aattattttt tataattctg gtatactttt
    16681 ctttcctttg taccacaact cttctggcgt atacctgtct tttccattta tgaatactgt
    16741 tccatctgct tcaatcgtta cttcagtccc aatccacata ttacgagaaa gtgcgagaag
    16801 attttttctt tttcctcttc ctttttttcc aaattgcaag gcatcggtta tctcttctgt
    16861 ccaatgagat ttcacgtatt ttacttctcc gttttcaaat gtaactttca aaatattcgc
    16921 tatattttcc acttttactg ctttaggaaa tgactccttc atttttttca ctttccttcc
    16981 tccaacacta agtatgcgct ttcttttagt gataagtata tcatagttta ttctattatc
    17041 aaaaaaacgc atccattcca ttgtagaata ctttaccgaa caagttgaat tgttttcctc
    17101 tttttcacct tttactatag ataggtaaag acaactaagg aggaatctct catgacagat
    17161 caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa cacttccagg tcaatatcac
    17221 gaaacagata ttgaaatcga tcctgaagca gaattatctg gtgtttaccg ttatatcggt
    17281 gctggcggta cagtaaaacg tccgactcaa gaaggaccag ctatgacttt caacaatatc
    17341 aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg ctagtcgtaa acgtgtaggt
    17401 gaaattttgc accatgatta tcgaacactt ggtcgtttct tgaaagacgc tgtagaaaat
    17461 ccaatcaaac ctgtgaaagt agacaaagca gacgctccag cacaagaagt cgttcatcta
    17521 gctaccgatg cagacttcga tattcgcaac attttgcctg cacctacaaa tactcctgaa
    17581 gatgctggtc catacatcac aatgggtgta gtcttaggat cagatccaga aaaaacaatg
    17641 agcgatgtaa cgatccatcg tatggttcta gaagataaag atacgatcgg tatgtacatc
    17701 atgcctggcg gacgccacat tggtcacttc caaaaacaat tcgaagcctt gaataaacca
    17761 atgcctatca ctatcaatat tggtttagat ccagcaattg ccattggcac gactttcgaa
    17821 ccacctacca ctccacttgg ctacaatgaa ctatggtgtg caggagcttt gcgtaacgaa
    17881 cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg gtattgctcg tgctgaattc
    17941 atcattgaag cagaaatcat gccaaatgaa acaatgcaag aagatatcaa cacgaactca
    18001 ggaaaagcaa tgccagaatt tcctggctat actggggatg ctaatccagc tgtacaagta
    18061 gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa tcatgcaaac aacgattggt
    18121 cccagtgaag aacacgtatc tatggccggt attcctacag aagcaagtat tcttgccttg
    18181 gtcgataaag ctattccagg aaaagtagta aatgtctaca atcctcctgc aggtggtggt
    18241 aaattgatga ccatcatgca aatccgtaaa gaaaacgaag cggatgaagg tattcagcgt
    18301 caagcagcta ttcttgcttt atcagccttc aaagaattga agacagtcat tttagtcgat
    18361 gaagatgttg atattttcga catgaacgat gtagtttgga ctttcaacac tcgtttccaa
    18421 ggagataaag atatcatcgt gatcccagga atgaggaacc atccattgga cccatcagaa
    18481 ttaccagaat ataatcctgg ctctattcgt ttccgtggga tgagtgcaaa aactatcttt
    18541 gacggtacag ttcctttcga tatgaaagaa cattttgtac gtgcaaaatt ccaagaagta
    18601 gaagattggg aaaaatattt gaaataatga atgatctgca actgttttga catcataaaa
    18661 aggagtgcgg aaaatggaac aattggcaca acggaagaaa aaaatcgtga ttggtgtaag
    18721 tggcgcttca ggcaccattt atgccatcaa cctagtgaag aaactaaaag aatatccgat
    18781 gatagaaaca cacggaatca tcagtccatg ggcaaaacag aacttaaagc tggaatccga
    18841 cttatcacta actgaattcg aaagcctgtt tgattaccat tattcaaaca aagatttagg
    18901 tgctgctgtc gccagcggat catttttgac cgatggaatg atcatcgtac cggcaagtat
    18961 gaaaacagtc gcaggtatct ctgttggttt aggcgacaac ctgattgcac gtgctgctga
    19021 tgtaacaatg aaggagcaac gaaagttagt cattgttcct cgtgaaacgc cattaaatgc
    19081 cattcacttg gaaaacttga ctaaattagc acgactaggt gtgcaaatca taccaccaat
    19141 tcctgctttt tataatcagc ctcaaacgat ccaagatctt gttaatcacc atacaatgaa
    19201 acttttggat caatttggaa ttgtaaatga gcattcaaaa cgttggcagg gtgattaata
    19261 tggaaaaaga aacgatagtt gaagtaacca aactaggttt cacgatccaa gctgctgtta
    19321 aagtaatcgg taaagactta ttgatcactt taacaggcgg agatacacca catatcggaa
    19381 cagtcactac attttctcgt gatacagaga tgcaaactgt acgtttccct agccacgatg
    19441 gtcggttcca taaagacgat gtcttagccg aaaaaatagc tgtgatcatc aaaactgccg
    19501 ttccgggaag ttgtacgatc acttccggta tccatgttga ccatatctcc aatgcacaaa
    19561 tcaacgcctc ttttccgatg tcagaagagt taggttacaa gattttagat tggttggaaa
    19621 aaatagattt ccatatcaaa gaaccagttt attataaaga tggtgaaaaa acattatgaa
    19681 agaagaggtt gtgaaatcag cgttatgacc tgagtatgag agaaaaaaat ggaaaaatag
    19741 cttctcatat tttgccgttt ttgagctaat accgagggac agctgattga acgccgttta
    19801 tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa gaaaaatttt gtaaacatgg
    19861 gtttccacat ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt
    19921 tatcaagagg ttgtgacacg tcttttgtca caacctcttt tatttttgct tcactgtaac
    19981 tgctacaata tcaccgattt cttttccgat gtttttacga atatcttttc gtagtccaag
    20041 gatgtagcag actgaaccat ctggattttt cacgcccata ttcacgatac tgccttcata
    20101 cggcatattg tcaaaagttg cttggacttt aactcttcca gcaccgaatt cttttcttat
    20161 atcatacgga aaaataatat atgccccgcc cttgtcgact tcggaagcat agatttttga
    20221 ttcaaaagaa taaatttccc tcattttatt tctcctattt tttacatcta gtatagcata
    20281 gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag ttcctttttt cgaataaacg
    20341 gtgttctcct gccaactcct cggtcacaag tcacaatagt caacaaacat gagaagctgt
    20401 tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacaacc tcttccttaa
    20461 acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa atatggagtg
    20521 ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac aacctcctac
    20581 atggtgttca aaatattatt cgccttttgc ttctttcact aattttgcga atgcttgttg
    20641 ttggaattca gcagctaagg ctttcgtacg ttgttcttgc tctgctttgc tcatttttgg
    20701 attttcagtc attgctttta ccataccgtc ttgaacataa gcttttgctt cactttccat
    20761 cgtactttgc tgtttcttca ctaattcagc taacttggct gcttggtccg tacttaattc
    20821 tacccatgtc tctgggattt tgaatgtatt cactcggctg tcatattcgt gattgttatc
    20881 agcaatattg aaccagaatt tatacatgtc gtttttgtat tcccaataag tagttttcgt
    20941 cactttttca gcatcgccgt ctgtcttttc tactttattt gtttcgttat ccgtaccagt
    21001 tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga tacacttttt cttttccatt
    21061 ccctaaagct ttatacaaaa gcatatctgc tccttgggaa tcagcactgg aaacaatttt
    21121 ttctgttttt gtttccgtta ctttttccat tccaaagtga ttggacaaat tagcgacgat
    21181 caaacttagt gaagcgacga agattagtcc gaaaatcaaa gataaaaagg tttgccatgt
    21241 ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag ctgacaatca aaactccaat
    21301 aatcatgcat tatcccccgc tttcattaga acaggttctt ttttcttcaa taaaaaggca
    21361 ataataaagc caagaatccc aaagatgatc gctacgataa acgcggcatg gtaacctgac
    21421 agtgtggcat tgattgcctg atctttataa gctaatggcg tattatccaa tacagatttg
    21481 cttgggatat ttgcttttgt cacattcgtc aacacactga tcaaaatagc tgtaccaatc
    21541 gagcttgcga tttgacggaa tgtattattt accgctgtcc catgactgat caagttattt
    21601 ggcaatgcat tcataccaga agtcgttacc ggcatcatga ccatggagat cccaaacata
    21661 cggatcgcat aaaggatcac gatatataaa actggagtcg tttttgtcaa gaaagcaaat
    21721 ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg ctaaacgttt cgcgccgtat
    21781 ttatcgaaaa tcgcacccgt cacaggcatc atagccccca tgatcaatgc acccggcaac
    21841 aacatcaagc cagaatggaa tgctgattct ccacgaatat tttgaatata caatggtacg
    21901 accatttctg cccctaccat cgccatattt gttacaccag ctaaaacagc tgcaatcgtg
    21961 aatgtagatg atttgaatac acgaagttcc aagaatggat gttccaaatg taattgacgc
    22021 catgagaaca aggcgatgaa aatcacacca acaactaaga aaccaattac tttcaagctt
    22081 ccccagccgt catctcctac actagaaaat ccgtaaagta aactaccgaa tccaatcgtt
    22141 gataaaatag cggataaaat atcaatgcta ggatttgaca atggaataac acttttcatt
    22201 aggaagaatg ctaaaaccaa gacaagaaca acgattggca gtaccatacc aaacaagtca
    22261 cgccaatggt agttatcaat gatataacca gataatgtag ggcctaaagc aggagcaagt
    22321 ccaataacga tccctgacat ccccattgct gatcctcttt tttctggcgg gaaaatagat
    22381 agcatgatat tttgcaataa tggcatcgat actcccacgc cgcaagcttg gatcaagcga
    22441 ccgattagta atgtactaaa attaggtgct gtaaagcaag taatcgttcc gatcaaaaag
    22501 atcgtcatgg cagaaagata taaatttctt gaactgaatt tattgatcag ccatgctgtt
    22561 attgggatca tgatcccgtt gactagcaag aaaccagtcg ttaaccattg cacatcagac
    22621 gctgaaatat cgaaagcttt catcaatgta ggaaaagctg tcgtcaataa ggtctgattc
    22681 aaaacggtac aaaatgtacc aattaataag acaacaacta aaagtgtccg attatatggt
    22741 tttccgtaaa tatctacggg ttggttgtta ctcatatttc tccttctttc tgaatttttt
    22801 ctgaaactac tagaatactt tagtcctaaa ttcgctattt gtcaacctac ttgacacata
    22861 agttaatacg gatataatta agggaagcaa gaggacaaaa ggagtgacaa aaatggttgg
    22921 ggcttccctt aaacgtttgt tggaaaatga tcaattggtt gttggcatca gtgaattaag
    22981 tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg gaacaaaagg gattcattcg
    23041 ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt ttacctacag tcataaaagt
    23101 agaactgatc aaaaaatatt tagatgacgg atttactttg tcaaaagctg cagaaaaggc
    23161 agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt ttttcaaaag caatcaaaga
    23221 tatcgaattg atcgacgaac gctatacgat tttacaaatc ggtaacttca aagaagagaa
    23281 tgctaagatg tacatcattc atgacaacga gacagacgaa ttaacttaca agctccttcc
    23341 tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa ctctaaacgc cactaatctc
    23401 ctaagtacca agctttttaa taagtagatg ctacataaat acttgaaaaa aggcctgttt
    23461 gaacataaga atatccgaac ggtgatctgc gtttctttca tatagaaagg aattctccat
    23521 cactgttcgg atattttttt atttttttcg gttactttct tctaatacat ctgttagcca
    23581 atttgcatat tctgtttccc gattgatcgc atgcgctaaa atcaggtagt gtccgtaatt
    23641 ttcatgtttt tctttttcag taggaaagat cgtttttttt cgctctaata gatgaagtcg
    23701 tttttcttca tgaaggtcac gctgctgctg aataatttct actaatgccg gatcattcac
    23761 gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga ttaaccggta gttcattggt
    23821 tggtgtatga atccactctg ccaattgttc cttcccttct tctgttaatt cataaacttt
    23881 cttttccaat ttttcacctg tgatctctac ttgatgctga atgattcctt gctcttccat
    23941 ttttgcaagt tcaggataaa tctgactatg ttttgcttgc caaaattctc ctatttcatt
    24001 tttgaacgat tgattcaatt catatcctgt catcttcttc ttacttaata accctaacaa
    24061 aatatatcga agtgtatttt ttcttgccat cagttttccc tccttgacaa atctttaaat
    24121 ccattctatc ataaatgagt aatcgaaaac atgtagttta ttacatgttt taatttataa
    24181 ggagatgctt aaacatgaaa gaatttaaag aattggaaga ttttattgga actcacttta
    24241 tctatacgta tgacaatggt tgggaatatg aatggtatgc caaaaatgac catactgttg
    24301 attaccgtat ccatggcgga atggtcaaag gccgttgggt aaaagaccaa gaagctgata
    24361 tcgttaaatt gacggacggc gtattcaaaa tcacttggac agagccaaca gggacagacg
    24421 tggcattgga ctttatgcca aatgaaaaga aacttcacgg aatgatcttt ttccctaaat
    24481 gggttgaaga acatccagaa atcactgtta ctttccaaaa cgaacatatc gcagaaatgg
    24541 aagcagctcg tgaaaaatat gaaacttatc caaaattact tgttcctgaa tttgctacga
    24601 tcacttatat gggtgatgca ggtatagaca atgaagatgt tattagcgaa gaaccatacg
    24661 atggaatgcc agatgatatc cgtagcggaa aatacttcga tgaaaattat aaacgagtaa
    24721 aataaggttg tgaaagagag gttgtgaaat cagcgttctg acctgagtat tagagaaaga
    24781 aacggaaaaa tagttcctca tgttttgccg ttttttgagc taataccgag ggtcagctga
    24841 ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat gctttgagta ataagaacgc
    24901 tagttagaaa atagcttttc atattttcta ggctagtggg cttattaccg agaagcagcc
    24961 gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa
    25021 acaaatggaa aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc
    25081 tgattgaaca ccgtttatca aagattgtaa caactgcata ttcacacagt taaattagtg
    25141 acttcaaaat gaaataatgc tcccagcaaa aaattaggct gggagcatta tttcgttatt
    25201 ctgttcatca aaaatcagcg ttttgattcc atttcatcac aagttcctcc tcatttactg
    25261 cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga aagatagaaa tgaatagctg
    25321 attgattttt ttgatagact gtcaaagtca gtgttttatt ggtttctttt atttttttca
    25381 ttagttgcgt accgattctt tgttgccggt attccctttt cacaaagatt cctgcgataa
    25441 actgatcaat caccccagca aaaccaataa tttcttgatc tatttctgct aagtaaatca
    25501 ctgcatctgg taaagctttc ctaacagatt ctacttgatt ctcccaataa gttgatggaa
    25561 tgaattcatg agaatccaag ttagcatgca gccaaatcga tagaacagat tccagttctt
    25621 tggcagacaa ttttgttgtt gtgcggatca ttttttctct ccttacttat tcattgattt
    25681 taaaataaaa accatttttt tgaagctctt ttacaagttt tcgcccctca aaaaagtata
    25741 tcttctcttg ttttactttt ctaataccaa aagaaacagc aactttagga tcatcagctg
    25801 aaacgattcg ataagttcca ccaggcaatt gatccagctt gactttccca tgatggtcgc
    25861 tttgtactga aaacggcaca ccatttctag taagtgtttt tttcttatgt ttcaaaatga
    25921 atgaactacc atttaccggt ttccctgacg aatcactaat aataaatgaa aaatcaagca
    25981 cttttttact taatctatac tgatagatta gtcgtccgat aaacactaac agtatgacta
    26041 cagctattat cgttcctgca agaataaacc attgccgcat ggttctttcc tgatttcctt
    26101 ttccttcttc tttttttacc gattcgttat aaggaatccg atgtcctgtc actagcaaac
    26161 gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt aactaagtct tctccttctg
    26221 aaatcgttag aacagacgtg tcatctggca gtaccacttg tatattttta acttgataag
    26281 ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc gttttctaaa cgatctaatt
    26341 cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga gatcactgaa tgcgtcgaat
    26401 ttccaccaag cgggaaagag gttccttgca agacgcctgc tccattttca aggatcctac
    26461 cattgagccg atcataaagt gaaatattta attggatctt aggtatattg atacttccga
    26521 tcagccattc ctctaattgg ctggatgttg tactttcatt tctgttcttt tcattgaaag
    26581 gatcagtacc aggattgaat ccttggtcag ttaatcgttg ttttttctcc tccattttct
    26641 ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac ttgttcgata cgtttttgat
    26701 caataaaact atttaaagaa tccacataaa atggatataa agcgatcagt aaccctacga
    26761 cgatacatat taataaaaac aaacgaaaca aaagtcgacg agactgtttt tgcttactct
    26821 gcttcacatg tgtcctccct tgcaagtttt tttgttcttt cttttattat atgggttcag
    26881 gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa gctgtcctgc atcaagtaat
    26941 aagtacagcc caaaaaaata gctactcata ttttttgttg ggttgggctt attaccgcag
    27001 atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa aactgcgtta tgctttgagt
    27061 aataagagcg ctagtcagaa aacagcttct catattttct aggctagtga gcttattacc
    27121 gagaagcagc tgattgaaca ccgtttaccg agaggtcatg aaaaagctac tttgtttcga
    27181 gtattaagaa aaattctgtt tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc
    27241 tctccttaga actttcactt atgtttttct tgtaaaattc ataaatattt tcatgaattt
    27301 tctaatttct tgcctacctc tcgcatagga tatatgatga atttatctaa agtttgcaaa
    27361 ggagttcgac aattgaaaaa atatggccca tttttagcaa tttttttcat tgctttggca
    27421 tttctcatgc ctcatttatt cacacataaa ctaattttgg gtgctgatgc cccattccat
    27481 tacaatcggt tctatgatgc cgcgcaacaa attaaatatg gggatttcca ttattttcct
    27541 tccctttatg gattccagca aagtggcagg atcgtgaatg ccgtttacgg cccatttttc
    27601 gcttatttac aaggattgat cgtgttgctt gcaggtagct ggtttcatta tcagattctt
    27661 tcaaatttca ttctttatct tatttcagga ttttctatgc tgattttact gagatatgtc
    27721 aatgttcgaa attgggtaag tgtaccagta gccgttttat tcatgagtac gtattctatt
    27781 caatactggt ctatcaacca aggattcact agctggggga cttgttttta tcctttatgt
    27841 atgatcccta ttgctgattt cgtccttcat aaaaagttcc ctgttatcaa agtcgctgtc
    27901 tcagtgggac ttatgacaca gatccattta ctgtcttcga tcatgttagc tttgatgtat
    27961 atcccttttt atctttatta cttttttaac cagactgaaa aaggaaaagc actgcttcaa
    28021 ctgattaagt cggtcttgct ttattttgtt ctgacagcaa atatttgggc aggattagct
    28081 attgtttaca gtgggaataa gattcttcct ccctttgtta acagagaaat gagtcaaaag
    28141 gcgatagatc tggatggcag ttattggctc cattacccta aggcctttcc tatattgata
    28201 ttatcaggtt ttttgctttt tattttcttt caacaaaaga acaccttatt ccaaaaaatc
    28261 gtgttgtaca caagtcttgt tttctttttt ctttctacta gcttgattcc atggacgaca
    28321 ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt ttccatttcg atttttcgtg
    28381 ccatttacgc tattgcttct actttacctt gccctttcac tgaaccagtg gtctgaacaa
    28441 aagtggttcc gtcttttaag tatatttggc attcttattt tttcagcaca gacgatccag
    28501 aacttatacc agcatctgga aaagtgggaa aatgaaacat ttgtcagtcg acatacttat
    28561 ctattcgata cgccggaaga agcaaggaaa acattcttca gcaaagatat gtctaccagc
    28621 ttgttcgttt tccaaaaaac cacacctgac tatctgccaa tctataagga gacaaaagac
    28681 aataaatacg accggtataa cgaagaaata ctagaaaacg aaacaacttt tataaaaaca
    28741 catccaggcg gtcttttgac aatcaagtgg aaaaacactg gggacaaaac tgttcatatc
    28801 cctgttatcg tctacgatcg gactgtgcta cagcaaaatg gaaaaacatt gactgattat
    28861 gaagtaacag atatcgggac cccaatcgtg aagcagaaaa aaggaatcaa cgagctcacg
    28921 cttcattatc aaacgccaat ctatttctat ttcattcttt ctcttacttt gattggctgg
    28981 tttaccttgc tatgtttatt tatttatcat agatacaagc ttttacgtgc ttaggcaaaa
    29041 gggataaata catccgatca aaaaactctc caaacggtat aatcgtttgg agagttttat
    29101 tttaatcagt ttttttatgt tatatgttat attttttatt cgttcacatc taattcgatt
    29161 tccaccccgt agtgatcact gatgattgga ctatcgatcc catcaaaaat ccgttgatat
    29221 tttttaacct gcattccttc agggacgaaa ataaaatcta tacgcaattt ttctgtattt
    29281 tgttcccaac catctatttt cttttccaca gtagctgctt ttcctttttc attcgcgaca
    29341 acatacgaat cttgaagaag aaggcaactt tctcttacag tataataacc ttgagagtct
    29401 actggattat tgaaatcacc taggaaaaaa agtggtacgc gagtttccaa aaagtagttt
    29461 tccaagtttt tccactcaaa ataaaagcca ctttttttat ctgtccacca agaaaagtgg
    29521 cagctcgaga caaaaatgtc ctgatcatct atctttgttt tcccaatcaa gatcttgcgt
    29581 gtctgataat cttctttctt ttgagattgg gagacggtaa ggacctgact ttccaatgtt
    29641 tttttcgtaa gcagtgcatt tccttcttca tatttatcat aaccgatgtg gctcatttcc
    29701 caactccaaa aatactcttt tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa
    29761 ttatctttac ggataggtac ttgattcttt acctgacaga acttcattag cttcgcaggt
    29821 tctacttctt cagattccag taattggttg atttcttgta aagcaatcac ttcataatct
    29881 tctaacagga tcttatctgc taattcttgc aattttttct ctggttctgg ctcaagccag
    29941 ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa
    30001 aagggttgtg acaaaatttt tgtcacaacc tctccagcaa atagtgttct cctgctaact
    30061 cctcagtaat aagctcaatg gaccaggagc tattttttgt ttggctgttt ttatcacttg
    30121 gtgcagggca gctttttcac gacctctcca ctaaacagta ttcaaactgc tgcttctcgg
    30181 taataagccc aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttatgac
    30241 ttgatgcagg acagcttttt cacaacctct ccttacattt taatggagcc gatcacttta
    30301 ccagctgtca ctgtacctaa ttgcttgatc actacttctt ctactttatc gtttgtaaat
    30361 acgatcatga tcgttggatc tttttcttcg ttttggatca catcaagatc catctcagct
    30421 aatttcgttc ctgttcctac ttcttggcct tctttgacag caatctcaaa agctggctga
    30481 ttcatttgga ctgtatcgat tcccatatgg atcaaaactt cgataccatc tttcgtttcc
    30541 agcccaattg catgtttgct agggaaaata cttgtcactt ttccagcgat cggcgcaaca
    30601 acttctcttg atgttggaat aacagcaaag ccgtcaccca tcattttttg tgagaatacc
    30661 ggatctttta cttcagataa aggaatcact tctccagaag ctgcggaaac tagttcttgt
    30721 tctataccta agtattgctt actacctgct tgtccatttc ctgtagaaac catttctggc
    30781 tctggaatct ctgctcctga ttgcagcaga tcttcgatat ctgatttcaa gacatctgct
    30841 tttggtccat aaactgcttg gactccattg tctttgacga tcaatcccat cgcccctgct
    30901 cgtttccagg cttcttcagt ccctactttt tctcgatcat ttacacttac gcgtaaccga
    30961 gtcatacaag catcgacatc tttgatattt tcttttcctc ccagcaaatg aatgatctgg
    31021 accacttgct gatctgctgt tcctgttccg ctgttagata caacggtttc ttcactgtca
    31081 ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga attttttgat caagaaattt
    31141 gctaagaaat acgtaacaac accaaaagcg atcacacaca acacgaagtt gataagatct
    31201 ccacctagac ccgctttgat agctaacggt gtccgagtca gcaattcgat attcccaaat
    31261 gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg cagccccttg gatcactgaa
    31321 taaatgacat ataatggcac agcagcaaac ataaacatga actccaatgg ttctgttaca
    31381 cctgttagaa agacagccaa cgctgctgaa aaatacattg atttatatct tgctttctta
    31441 tcaggatcga cattacgata catcgcaaaa gccattccca tcaagatacc tgaagaacca
    31501 atcatttgtc ctactttgaa acgagccggc gtccagttat gcaacacaaa ctcatattgc
    31561 gacatatccc ctgcaccttt aagattcact aaatcagtgg cccaagccaa ccataacgga
    31621 tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata aaatctcata cgatccgcca
    31681 agctgtgtat agttgatagg aatcgtcagc atatgatgca atccaaatgg caatagtaaa
    31741 cgttctaatg ttccaaataa aaacggtgct agaatcggtg cgctttcttg tgattctgca
    31801 atccataaac caaaattatt gattccagcc tgtacgtttg gccacaccac agcaagaatc
    31861 aatgccacga ttgttgacca taagataaca acaaacggaa caaagcgctt tccattgaaa
    31921 aatgacaaag catccggcaa ttttctataa ttatagtact tattataagc cattgccccg
    31981 acaaatccag cgatgatccc tacgaatact cccatattca aagctggtgc ttccaataca
    32041 cttgtaaaaa atcctttaac catgatcttc gtaccgaaca gagtgtgggt aaatgcatct
    32101 tcattcgcaa gcatgtcact tgtgacacca aagatagccc cagtgatccg attgattaaa
    32161 ataaatgaga tcccagctgc aaatgctccg cctgctcttt cttttgccca acttcctcct
    32221 attgctaaag caaataaaat atgaagatta ccaataattg cccaaccaat attttcaatt
    32281 actccgcccg ttgtgacaag cggcgtccaa tcaggattga tcaagggaat tgatttccct
    32341 atactgatca tcaacccagc tgccggcata actgctacca caaccattaa agcctttcca
    32401 aatttttgcc aaaactcaaa actaaatatc tttttcattc cgtttagctc ctttacgcaa
    32461 tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt tgtaaatgct ttatttttta
    32521 tttttctgtc tataccattc tataaaacct ataatacgtg tgttcccttc tataattttc
    32581 tcctaaaaat ttattttttt aagctttttt ctatgaaata ttttcttttg tctattgact
    32641 tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc tcattaggag gagaaaaatg
    32701 aaacagatca aacgactatt tgctattgat ccatggaaaa tcagcacgac acaattagat
    32761 aaaaaaaatc ttcgactgca agaatcttta acaagtattg gaaacggtta tatggggatg
    32821 cgcgggaact ttgaagaaac ttactcaggg gaccaccatc aaggtactta tttggcgggt
    32881 gtctggtatc ccgataagac acgtgtcggc tggtggaaaa atggttatcc agaatacttt
    32941 ggaaaagtga tcaacgcctt gaactttatc gcgatagata tttcgatcaa cgatcagctg
    33001 atcgacttgg cttctttgga accagaagac ttttactggg aattagatat gaaaaatggt
    33061 gtattatctc gtacattcac aatcactact tctactaata aagtacgcat ttccttcgaa
    33121 cgttttctga gcattgtcaa aaaagaatca gcttatatct atttaactat agaaatgctt
    33181 gaaggtagcg gaacagtcaa actcgtatca aaattggatg gaaatgttca aaacgaagac
    33241 agcaactatg atgaacattt ttggaatgag attgatcgtg gaactataga agaaatcggc
    33301 tatttgacga ctaaaactat tccaaacaac tttgggattg aacaatttac tgttactgca
    33361 gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg actatcacga agatacttta
    33421 gcattacatg gttgcttgtc tgcagaatta tctcaaggtg agacatggtc agtggaaaaa
    33481 gaaattatcg ttcttactag tagagatatt ccagaagcgc agcaagcaga aaaagctgtc
    33541 tcagaattaa cgaaccagac aaacaattat atccaagcaa aagaagagca aacagcggct
    33601 tgggcagaac gatggaagct tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa
    33661 ggtattcgat tcaatttgtt ccaactattc tctacttact acggcgaaga tgaacgattg
    33721 aatatcggcc ccaaaggatt cacaggagaa aaatacggcg gagccactta ctgggataca
    33781 gaagcgtatg ctgtccctct ttatcttgct ttagccaaac ctgaagtaac taagaatctg
    33841 ctgaaatatc ggcataatca actgccgcaa gcaatccaca acgctcagca acaaggatta
    33901 aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg aatgccataa cgagtgggaa
    33961 atcacattcg aggagattca ccgaaatggc gcgattgcct atgcaatcta caattatacg
    34021 aattacaccg gcgatacgga atatttgaaa aatgaaggac tggaagtttt agcagaaatc
    34081 gcacgcttct gggcagaccg tgtccatttt tcaaaacgag caaataaata catgatccac
    34141 ggtgtgaccg gcccaaacga atatgagaac aatatcaata acaattggta tacaaatacg
    34201 atcgcagctt gggtcttgcg ttacacgcga gaaaattatt taacttatca aaaagaagct
    34261 gctatcacta tcagcgaaga agaactagcc aaatggaaag atatcgcaga gaacatgtac
    34321 ttcccagttg acgaagaatt aggaatcttt gtccagcatg acacattctt agataaagat
    34381 ctcatgcctg tatccgatat tcccacaagc gatcttcctc tgaatcagca ttggtcttgg
    34441 gacaaaatcc ttagatcttg cttcatcaag caggcagatg tactacaggg gatttatttc
    34501 ttcaatgata cttttacaaa agaagaaaag caaaaaaact ttgatttcta tgaaccaatg
    34561 actgtccatg agtcttccct ttcgccaagt atccattcga ttttagcagc agaattagga
    34621 atggaaagca aagctgtaga aatgtaccag cgaacagctc gcttggattt ggataattac
    34681 aataacgaca cagaagacgg cttgcacatc acttccatga ctggtagctg gttgtcgatt
    34741 gtccaaggat ttgctcaaat gaaaactgcc catgatcagt tgagattcgc accatttctg
    34801 ccaaaaactt ggaaagctta ttctttccat atcaattatc gcggacgttt attgttcatc
    34861 tcagtcacag atgaacaagt tactttcgag ttattagaag gttttcctct agaaatttac
    34921 gtctatgaac agtcttattc tttagcggat cgtctagaaa taacgctaag aaaggaggaa
    34981 gcaaatgttt aaaggggtat tgtttgattt agacggtgtc attaccgata ctgctgaatt
    35041 ccattatcgt gcttggcgaa agcttggtca ggaaatcggc attacgatcg accacacatt
    35101 caacgaacag ctaaaaggcg tcagccgaga agactcactc tctttgcttt tagcttatgg
    35161 cggaaaagaa cactcttttt caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga
    35221 ttattattta gagatgatcc agacgatcga accaaaagat gtcttccccg gggtcgtgcc
    35281 tttactggat tctttaaaag aagaaaaaat caagattgct cttgcttctg caagtaaaaa
    35341 cgggccattc ctacttgaaa aaatgggtct tacaccttac tttgatgcta ttgccgaccc
    35401 ggcagaagca acaaacggaa aacctgcgcc agatatcttc ctccttgcag caaaagctgt
    35461 cggactaaaa gcagaagaat gtatcgggat cgaagacgcg caagccggta tccaagccat
    35521 cctctcaagt ggcgctcaac caattggcgt gggacgtgct gaagaccttg gagaggagat
    35581 cccgcttgtc cctgatacga cttttcttac gatcgattac ttgaaagaaa agtgtcatga
    35641 ccatggataa tccagagatc tccgtcgtac cagttccaga gactgaaata aagaaaatca
    35701 cattgaagaa cgactactta gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa
    35761 tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg
    35821 aagatgttct aacagacaaa agcttttttg gagcaactgt cggacctgta gccggaagga
    35881 tccgggatgc ttcttggggt gagcatagat tggagaaaaa ctgcggatca caccatatcc
    35941 acggtggcac gaaaggctgg tctttccaat tttgggaagt agaagttttc aaaactccat
    36001 catcaatagg tgtggtcttt tacttaaaag atgaattttc tacctatcca ggaccaatca
    36061 ctgctacgat cacttaccgt ttaacaaaaa acgaattaga gatgacaacc tctgctagta
    36121 gccttgttga aaccatctgc aatccgacca accatgccta cttcaacctt tcaggaaatg
    36181 gaacaagaga catctatgaa catcagttaa cggtcttttt agatggtgtg cttgaattag
    36241 atcaagaaaa actgcctact ggaaattgga caaaaaaaga aaaactgcca atcgacttta
    36301 gaaaatcccc aacactgcaa gagatccttg catgttatcc agatggacta gatgatgtat
    36361 ttcttttgca tcatcctagg ctttccaaaa cttccttaca attattcgag aagcattctg
    36421 gtcgccaaat gactatagca acttctaata aaagtatggt attattttct acgactggat
    36481 tcgaagctga tttctctgtc aatggaaaac agatgcattc aaactacgga ttagcaatcg
    36541 aaccgcaaga aatccctgat atcgttcatt tccccaaatt tggatcgatc aacctccatc
    36601 ctggtcaaga acggatcagt cagacgattt atcggttctc tgcacaataa caagagaaag
    36661 gtgtttttat catggctatt accgtaaaag atgtggcgaa aaaagccggt gttgccacct
    36721 cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc cgagacaaca aagaagaaag
    36781 tccgaaaaat tatggatgaa ctaggttatg ttcctaacat gactgcccgc aatctaggaa
    36841 aacgcatttc cagtgccatc ggcgtaatct taccccctct tgattcaaaa gagcggatcg
    36901 gtaacccatt ttatcttgag atcatggaag caatcaacga agaagcgcgg aattatgata
    36961 tgactacagc aatcgctact gctaaaaatt ttgaattgct gttagagaac gtccagcgta
    37021 tgcatttaca aaaacaagta gatggtttta ttctagtgta ttcagatagt caagatcctg
    37081 tcattgatta tttataccaa aaccacatcc ctttcacttt gatcggacag ccttatcatc
    37141 atgagaacga aatcgtttat gtagataatg ataaccagct tctaggtaaa caagcaacag
    37201 aatttttgat ccagaatgga cataagaata tcctgtttgt gacaaacacg actcatgaaa
    37261 gtttgtattt cgaacgatat ttcggttatc aaaaagcaat gatgatggct ggtcttcctg
    37321 cgttatccag tgtagtacta gagcatccgg aggattatct gtcctttgag aaaacgttga
    37381 aagaaaacga agccacagca ttggtggtca ttgacgatat cttcgccctt cgcacgatgc
    37441 agttggcgca aatgtacggc tatgtcattc cagagacact ctccgtcatt agcttcaaca
    37501 actcgatctt ctcaacatta acacaccctt accttacaag tatcgatatc gatatctccg
    37561 aattaggacg tgtcgctatg caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg
    37621 gcgtacgttt ggtcatccct catcgcttga tcaaaagaga gacagtcgtt cctttagaaa
    37681 acccatacta aatagctgac tcactaaata cgctgaccat ttatcctatg gtcagctttt
    37741 tatgtaaaag aagatactac ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca
    37801 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca
    37861 actccttcgg aaataagctg aaattcacaa aaatttaaaa agcaattttc ttgagtttct
    37921 tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttccttcc ttcaattttt
    37981 tacacgtcga cgattcaata aaaaaccgcc taatattgta aagacaggtg caagaatcga
    38041 gaaaaagacg aatggcaagt aatgcaacgc gctaactttc atcgttccca taatgaatgt
    38101 gccgctgact ccccacggta cgagagaatt gacggtcgca cctgaatccg ctagtgttcg
    38161 tgtcaaatag gtttttgata agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg
    38221 aagaatgatg gacagatact gttctccaac gatcagattg attccgatac agctcaaggc
    38281 agtaaacccg actaaacgag aaggtcggtc catcttttct tttaattctt gaacgatggt
    38341 atcaacgatc agatatttga tcagtaaccc acctaatgcc aatgcaagta aaatcaaact
    38401 agcagaacct aacatactca tgatcccgcc tcgagaaagc agttcatcta gctttggatc
    38461 aggtgtattt gcgacataac cattcatcaa gatgtctgct gcttttccga ccgtcaaact
    38521 tttctcatgg ataaaagaaa gaacgagagc agctgtcgat cccgctagta atgtagggat
    38581 agccggaatt tttttgattg cccccccaaa taaaataact agtggaatca atgtccaagg
    38641 agagatccaa aagctcgctt tcatgttttg gatcatttca ttgatactag ctagatcggc
    38701 tgaatgactg ttatgtccta atatcgtata aaaaatcaac gaaaggacga atgctgggaa
    38761 atccgtataa cgcataccag cgatatgttc gaatagatta actcctccaa tacccgctgc
    38821 caagttggtc gtatctgata aaggcgagca gttattccct aaaaaggcac cggaaacgac
    38881 tgcgcctgta gtcattgcat cattgaatcc aagtaaatga ccgatcccca aaaaagcaat
    38941 tccaatcgtc gaaatcgttg taaatgaact gccgacagtc actccgacaa ttgcacaaac
    39001 aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt ttaaatccat aaaccataat
    39061 cgtagggatg gttccagata aaatccatga agcaactaag acaccaatca aaagaaaaat
    39121 caggattgga atgatccctg gagtgattcc tttgatgatc ccgtcatgga tctcatccca
    39181 tgtgaacttt ttaaatctgc cataaaacaa caaacacatg aaagctgcaa ggatcgggat
    39241 atgtggcgag agctggaatc cgatgatcag tactcctagg agtgccaaca acacgatcaa
    39301 tacagcgata ctttctttta aagaaatatt tttattcatc gtcgatctcc tatttcaaat
    39361 gaaaaggccc tgccatgaaa aaactattca cttggcagga cctcctacta aatcagcgac
    39421 aaaacgtcgg ctgaaagact tatttttttt atttttcttc taaacttttt tctacctcaa
    39481 tcccccaaga atctttcttg aagtgattga tgatcaatgg aataacgaac atgaccactg
    39541 tgatagctgc tactaataat gcatcactca atgtcgctgt acctaatgta gcaaatacga
    39601 caacgatgat agagaagatc agcatgcagc tgactaccca agctaatccg tttccgtttt
    39661 taccaattcg gtaaggacgt tcgaattctg gatgtttctt acgcatttta agcaatgaaa
    39721 cagcaatcaa aatgtacacg atacaataaa taacagttgt agcatttgta agtgtcaaga
    39781 atacagcact tacgtcatcc attacaccat aaagcaaagc aaataatgaa ataacgattg
    39841 attgtgttag aacgacattt ctagatacgc cgtatttgtt ttctttatgg aatccgaatt
    39901 ttggtggaag gaatccttca cgagctactt ggatgattgt ttttgatggt ccagtaaccc
    39961 aagctgataa ttgtaaaagc acaccaataa ataccatgaa gctgaagata tttccgatca
    40021 cttctggtaa gcctaggatt tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt
    40081 ccatttttcc atctggtacg acatttgcta caagcatcgc attgatgaca ttcaataaaa
    40141 ctaatccgat caaagcgata aatacgcctt ttgtataatt ttttgttgcg tctttcaaac
    40201 gaggcatgta aacagaactc atttcgatcc caacaaaaat aaatgtgata ccagctaagt
    40261 atttcaatga atcaatatgt tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta
    40321 gatagccgtt tgagactagt ccgactttga aggctgccaa aacacctaaa acgaacatga
    40381 tgactactgg gatatatacc cctagccata caccgatgtt cccgccgact tttgccatat
    40441 cgaatttcaa gttcaagatc gtgatgatcc agtagataac taaaatacaa cctaaaacga
    40501 accagtgatt attgccaagt tctacattac caaatgtgtt tcctaacaat ggtcctaaag
    40561 tagaagcgac cattaccata cctgggaaca tttgcaccca taatagccat gcaacgacaa
    40621 atccccattt gctgcccagt gctgttttta cccataactg aggaccgcct tcttgaggaa
    40681 gcatcgtgga aagctcaccg gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa
    40741 aaattgaata aaaaatcaat gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg
    40801 ttgcacacag tgccattgtc agaccgataa aggtcccaag acccatttta cttacttcag
    40861 atttactcac cgttgacact ctccttgatt ttttggataa aataacggtg tctttgaagt
    40921 agtttcagca ataaacgaaa caattcagcg aatatttgag gggattcttt gagtctattg
    40981 cttgaagggt ttcttcaaag acaacctcaa aacaaaggga tcgaagagct taggtctccc
    41041 cttgctcttc agatccaaac atgcgtcaga aacagttagt attcaagctg ttttctaaca
    41101 tgattacctt gcttattttg cttcgcttgc caataattgg tctgcgtaga ttttttccaa
    41161 tttttcttga agagctgctt tgattttttc agcatattct tcaaagtgtt cttctttgtt
    41221 catgtatggt gtcattacgc tggcacgcaa tacagttact tttccggcac ggttccattc
    41281 ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg ctgttgccat agtctgggat
    41341 agcaaagtca gtatgtgaag ttaagaactc attaccgtaa atgcttcctt tcacgtatga
    41401 agaataatca tatacgtcat ggttcaattt attcattgca actaagtcat cgttgccttt
    41461 ttctttgaat acatagtcta ccatattgaa atctggataa gttaatggat gaacttcgat
    41521 ttctttgtct cctactttaa atgacaagtc attcaagaag ttgtagaaac gatgtgcacc
    41581 ttcgattgat gcacccatta atttaccata tcctgtgaca ttcaaaggta agacatggtg
    41641 tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa ccttccaaga tataagcccc
    41701 aagtaatgct gggatatctg cgcctttttc aaatacatat gtcgcaaagt aagagataac
    41761 gtctctcatg cggatatctt tgatgacgat accaccggca gaatatggaa catagcccat
    41821 tttatgtggg tcaatcgtta cagattctgc ttcttcgatt gctttatatg cactgtgtac
    41881 ttcttctaag atataatctt tgttttctgt aaagacattg tatttgtaat gaacatcttt
    41941 taaatcttca aatggaatga agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc
    42001 gccataagca gcatctacgt gtaagtagaa gtaaataccg tctttttcta atacgcgacg
    42061 caatgcaacg attttatcga taccgtcgat tgctccttct tctgttgatc ctacaacacc
    42121 gactacacct aggattggtg ttttttcagc tgctagacca cgaacgattt tttcaagttc
    42181 gttgatgtcc atgcgatagt tgtggtcaac tggcactgga atcacttggt ctaaaccgat
    42241 accgatgatg tcggcagctt ttagccatga atagtgttta gtttgtggaa ccaaccattt
    42301 tcctaatttt tctaaatgtt ttccgctacg tgctgagtga gctttgattt cgtcgatttt
    42361 ttctgggacg ctgtctaaca gattcatgat ttcttctgtt gacaagttca ttaattccca
    42421 atcgcttttt ccagcaacta gttcaggtgt cacttctttc attgcaagcg gcaatgattt
    42481 gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa gaaccatcag caacgatgtg
    42541 gccccagcca tctttatagc tcattaattt tgcaaattcc attcctactt cttcttccat
    42601 ttggcttgtt gcaggagaag attcataggc aacgttatta ccattccata gcatagcaaa
    42661 attgtacgct agtaaagatg gcatcaatgt ttcagagttc atatgacccc agtaacgtcc
    42721 ggcattatgc caaggaacgg aatgggtacg catccgcgct gaaatttctg atagaacatc
    42781 ctttgtttta ttcacagtgt gttcaaaact agcactgctt ttttcttctg gcgtaataat
    42841 aggcatgtct tgaggcatgt agttttgacg ccagcctaaa tgttcatcca ccaattcatt
    42901 caacaaagct ttatagattt ggccgttctc agctttgtcc ccaatgaata gggcatttag
    42961 gtttagatct ttcgacaatg attcactcat agtgaaaccc tcctaaaaaa atatttaaat
    43021 tttgtcgagc cctacattac acctgttctt ctattctgta aactaagttt tggcctgatt
    43081 tttaatcata ttttaatatg tatcaattat ccgaattgtt cacacttttg aaataattaa
    43141 aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa gattgtatta acgacaacca
    43201 gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt aatagtattt ccctttttca
    43261 aggtaaaaac ttactaaatt ctaatcaatt actttggcca aaaagtaatt tttcttacct
    43321 tttcgaataa ccacaaactt accatcgaat tcatcggatg gatttatgac aaaatttaaa
    43381 tcggtaacgc ggtcaccgtt aatacttata gctccgtttg aaacatcttc acgtgcttga
    43441 cgtttagatg gttcaatttt tgtggaaaca agcagttcca cgatattttc aggtgtgctt
    43501 gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga tttcttccgc atttagttct
    43561 ttaatatttc cagagaacaa tgcttgcgtt attttttgcg cttctttcaa gtcttcttca
    43621 ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt
    43681 tctgtctcta cttttttcgc taagtcttcg atttcttctt gagacaagaa agtaaagaat
    43741 ttcaagtatt tcactacatc gcgatcatct tgattcaacc agaattggta gaactcaaat
    43801 ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt
    43861 ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg cttctacttc tggtccttct
    43921 aattttcgga ttaggtctaa tccagctgtg atatttcccc attggtctgc tccaccgatt
    43981 tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt cgatagattg cagaatttgg
    44041 taagtaaact ctgtaaatga gattcctact tccaaacggc tggcaacgat atcttttgct
    44101 aacatcgtgt tgatgttgaa gtttttaccg tagtctctca aaaattcaag taaagagatt
    44161 tttgacaacc agtcgtagtt gttcacaaat gtgatatttg catctttacc aaacaatttt
    44221 ctcatttgat tggaaagtgc gtccacgtta tgttgcactt gttccatcgt ttgcaacaca
    44281 cgttctgttt tgcggccgct tgggtcgccg atcgttcctg ttccgccgcc aatcagaata
    44341 tatggatgat ggcctgccaa ttggaatctt ttcatcatca taaatggaat caaatgtccg
    44401 atatgcatcg aatcaccagt tggatcaacg ccgcaataaa gtgaaatgct cttttcttca
    44461 acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat
    44521 tcatcaataa tattcatttt actgacctcc gtgtagtttt tttatgaaag caggaacctg
    44581 ctagcatgtg taattaatta tttcttcagg ataggatagg aaataaaaaa agtcccttgg
    44641 catataagcc aagggacgaa atctcgcggt accacccaat ttgtatgtct agatatccaa
    44701 catacctctc gaacatttaa cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg
    44761 taattcgctt taaagcatgt actggtttgc agcgaccacc agctttctga aacattggct
    44821 ttaacaacta cttcgtttcg atcttcgtct tgttattatt aatatgtgtc tatcatagag
    44881 cgcaactttt tctttgtcaa cagaaaaagg atattttttt taaattcctc attttttctt
    44941 ttttgaatcc gctctaattc cgaacatttt attttcaatt attgttaaaa gaaataactt
    45001 tttctgtatt ttttctgcaa tttatcgaga agtcacttta ctttgcctct tattctttct
    45061 tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg
    45121 aaaaaaagca aaaaattatt ttaggagcca cttctgcttt aactatcgtt attttaattc
    45181 taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa
    45241 aaataacaga aaaagcaaaa gaaccgacag atgataagca acaactagtt actcaagcaa
    45301 aaaatctggc tcaaagctac gattatgacc aagcgattgc gttattggaa aaagatagta
    45361 agaacggatc tactgaaaca caacagcttc ttcaaacact aaaaaaggag aaagaacaac
    45421 tgatcgactg gtctgatcct actcagatct ctcatgtctt tttccacagc ttgatcgtgg
    45481 acccgcaaaa agccttccac tctcaacagg cacaaggata caaagattat atggtgactg
    45541 ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa tgattttgtt ttagttaatt
    45601 taaatgattt gatccaaaaa gacgaacaag gaaatctttc ctttaacggg cttgcgctac
    45661 ctgaaggaaa aaaaccattg gttctttcac aagatgatgt cagctattat gaatatatgg
    45721 ataattccgg ttttcctagc cgattggtcg tatcaaataa caatgaaatc aaaaatgtct
    45781 ataaagagaa agataaagaa gaaatcggtg actttgacat ggttcctctg atcgatgcct
    45841 ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa aggtaccttg gccctgacag
    45901 gttacaacgg agttttaggt tatcgaacat caaaatctca atatggagac aatgaaaaaa
    45961 caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca gttgaagaaa gacggctgga
    46021 gctttgcttc ccatacatgg ggtcacttga atatgactca agcttcacta gaagatgtaa
    46081 aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat cctaggaaaa acaaatattc
    46141 tgatttatcc atttggtgca gacatcagcg actggcagcc ttattcagca gaaaatcaaa
    46201 aatttgctta cttgaagagt caaggtttcg atattttctg taatgtcgat gcttctacac
    46261 cagcttgggg acaattaggt acggatttct atcgaaatgc tcggatcaat atcgatggca
    46321 ttcgttttca atctgattta gacggaaaaa atcctattct tgatccattc atcaatgtcc
    46381 atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa
    46441 aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt tatgctttat ttcttcttcg
    46501 gtgcgattcc cttgatcagg acttctgtcc attctgctgt atatggaagc agttttgcgt
    46561 atatctccgg cttagaaatt ccttctacca ttgcttgaaa atagaggatc aagaactcat
    46621 ccgaatattt caaatccact tttccttctt tccgccctcg atggaaaaga tcaagcatga
    46681 tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga aaattctttt ttttcttttt
    46741 ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt cactcgattg agataacgga
    46801 ttttgttttc actcatggca atcagtgttt cttcaaatgg aagattttcc gcaatcaacg
    46861 ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa tacttcataa atcagccctt
    46921 ccttgctgcc aaaatattta aaaatcgtcg ctttacttac ctgagcgttc tcagctacct
    46981 tatccattgt cactccagcg gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta
    47041 aaatctcttt ctttttctct tgtgctctct tttcaaaacc gttcatttgt agagcctccc
    47101 gtaattttaa acttttataa tatatatagt tcaa
//
LOCUS       DAPCMT010000007        45516 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPCMT010000007 DAPCMT010000000
VERSION     DAPCMT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 45516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.007"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="RD743_000299"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="RD743_000299"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDU0562160.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="RD743_000300"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="RD743_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDU0562161.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="RD743_000301"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="RD743_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDU0562162.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="RD743_000302"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="RD743_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDU0562163.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="RD743_000303"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="RD743_000303"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDU0562164.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="RD743_000304"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="RD743_000304"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDU0562165.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="RD743_000305"
     CDS             complement(8053..8523)
                     /locus_tag="RD743_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDU0562166.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="RD743_000306"
     CDS             complement(8569..8994)
                     /locus_tag="RD743_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDU0562167.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="RD743_000307"
     CDS             complement(9061..9330)
                     /locus_tag="RD743_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDU0562168.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="RD743_000308"
     CDS             complement(9341..10936)
                     /locus_tag="RD743_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDU0562169.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="RD743_000309"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="RD743_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDU0562170.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="RD743_000310"
     CDS             complement(14489..15049)
                     /locus_tag="RD743_000310"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDU0562171.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="RD743_000311"
     CDS             15402..16376
                     /locus_tag="RD743_000311"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDU0562172.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="RD743_000312"
     CDS             16546..17946
                     /locus_tag="RD743_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDU0562173.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="RD743_000313"
     CDS             complement(18024..18746)
                     /locus_tag="RD743_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0562174.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="RD743_000314"
     CDS             complement(18825..19331)
                     /locus_tag="RD743_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDU0562175.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="RD743_000315"
     CDS             19454..21253
                     /locus_tag="RD743_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDU0562176.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="RD743_000316"
     CDS             complement(21321..22895)
                     /locus_tag="RD743_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDU0562177.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="RD743_000317"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="RD743_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDU0562178.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="RD743_000318"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="RD743_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDU0562179.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="RD743_000319"
     CDS             complement(24672..25310)
                     /locus_tag="RD743_000319"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDU0562180.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="RD743_000320"
     CDS             complement(25311..25961)
                     /locus_tag="RD743_000320"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDU0562181.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="RD743_000321"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="RD743_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDU0562182.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="RD743_000322"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="RD743_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDU0562183.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="RD743_000323"
     CDS             complement(29104..29844)
                     /locus_tag="RD743_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDU0562184.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="RD743_000324"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="RD743_000324"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDU0562185.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="RD743_000325"
     CDS             complement(31215..32153)
                     /locus_tag="RD743_000325"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDU0562186.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="RD743_000326"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="RD743_000326"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDU0562187.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="RD743_000327"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="RD743_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDU0562188.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="RD743_000328"
     CDS             complement(35313..35618)
                     /locus_tag="RD743_000328"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDU0562189.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="RD743_000329"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="RD743_000329"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDU0562190.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="RD743_000330"
     CDS             complement(36724..37008)
                     /locus_tag="RD743_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562191.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="RD743_000331"
     CDS             complement(37026..37916)
                     /locus_tag="RD743_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDU0562192.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="RD743_000332"
     CDS             complement(37936..38154)
                     /locus_tag="RD743_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDU0562193.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="RD743_000333"
     CDS             complement(38124..39824)
                     /locus_tag="RD743_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDU0562194.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="RD743_000334"
     CDS             40091..40864
                     /locus_tag="RD743_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562195.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="RD743_000335"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="RD743_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDU0562196.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="RD743_000336"
     CDS             complement(41688..42659)
                     /locus_tag="RD743_000336"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDU0562197.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="RD743_000337"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="RD743_000337"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDU0562198.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="RD743_000338"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="RD743_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDU0562199.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
BASE COUNT        12398 a         9876 c         8236 g        15006 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagca
//
LOCUS       DAPCMT010000008        44898 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPCMT010000008 DAPCMT010000000
VERSION     DAPCMT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 44898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.008"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(13..1275)
                     /locus_tag="RD743_000339"
     CDS             complement(13..1275)
                     /locus_tag="RD743_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562200.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1268..2167)
                     /locus_tag="RD743_000340"
     CDS             complement(1268..2167)
                     /locus_tag="RD743_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562201.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2312..4090)
                     /gene="uvrC"
                     /locus_tag="RD743_000341"
     CDS             complement(2312..4090)
                     /gene="uvrC"
                     /locus_tag="RD743_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDU0562202.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4272..4586)
                     /gene="trxA"
                     /locus_tag="RD743_000342"
     CDS             complement(4272..4586)
                     /gene="trxA"
                     /locus_tag="RD743_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDU0562203.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4673..7033)
                     /locus_tag="RD743_000343"
     CDS             complement(4673..7033)
                     /locus_tag="RD743_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDU0562204.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7165..7695)
                     /locus_tag="RD743_000344"
     CDS             complement(7165..7695)
                     /locus_tag="RD743_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDU0562205.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            complement(7753..8190)
                     /gene="zapA"
                     /locus_tag="RD743_000345"
     CDS             complement(7753..8190)
                     /gene="zapA"
                     /locus_tag="RD743_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDU0562206.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8392..9309
                     /locus_tag="RD743_000346"
     CDS             8392..9309
                     /locus_tag="RD743_000346"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDU0562207.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9565..10476)
                     /locus_tag="RD743_000347"
     CDS             complement(9565..10476)
                     /locus_tag="RD743_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDU0562208.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10879..11457)
                     /locus_tag="RD743_000348"
     CDS             complement(10879..11457)
                     /locus_tag="RD743_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0562209.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11477..12145)
                     /locus_tag="RD743_000349"
     CDS             complement(11477..12145)
                     /locus_tag="RD743_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562210.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12160..13230)
                     /locus_tag="RD743_000350"
     CDS             complement(12160..13230)
                     /locus_tag="RD743_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562211.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13401..14765)
                     /locus_tag="RD743_000351"
     CDS             complement(13401..14765)
                     /locus_tag="RD743_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDU0562212.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14762..16321)
                     /locus_tag="RD743_000352"
     CDS             complement(14762..16321)
                     /locus_tag="RD743_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0562213.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDCPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16377..18857)
                     /locus_tag="RD743_000353"
     CDS             complement(16377..18857)
                     /locus_tag="RD743_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562214.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(18841..19176)
                     /locus_tag="RD743_000354"
     CDS             complement(18841..19176)
                     /locus_tag="RD743_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562215.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19190..20224)
                     /locus_tag="RD743_000355"
     CDS             complement(19190..20224)
                     /locus_tag="RD743_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDU0562216.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20314..20994)
                     /locus_tag="RD743_000356"
     CDS             complement(20314..20994)
                     /locus_tag="RD743_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDU0562217.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21278..22381)
                     /locus_tag="RD743_000357"
     CDS             complement(21278..22381)
                     /locus_tag="RD743_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDU0562218.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(23010..23480)
                     /locus_tag="RD743_000358"
     CDS             complement(23010..23480)
                     /locus_tag="RD743_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDU0562219.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            23810..25450
                     /locus_tag="RD743_000359"
     CDS             23810..25450
                     /locus_tag="RD743_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDU0562220.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            25604..26926
                     /locus_tag="RD743_000360"
     CDS             25604..26926
                     /locus_tag="RD743_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDU0562221.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(26994..27428)
                     /locus_tag="RD743_000361"
     CDS             complement(26994..27428)
                     /locus_tag="RD743_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0562222.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(27493..28330)
                     /locus_tag="RD743_000362"
                     /pseudo
     CDS             complement(27493..28330)
                     /locus_tag="RD743_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(29072..29698)
                     /locus_tag="RD743_000363"
     CDS             complement(29072..29698)
                     /locus_tag="RD743_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562223.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(29993..30328)
                     /locus_tag="RD743_000364"
     CDS             complement(29993..30328)
                     /locus_tag="RD743_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDU0562224.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(30315..30599)
                     /locus_tag="RD743_000365"
     CDS             complement(30315..30599)
                     /locus_tag="RD743_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDU0562225.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(30655..32178)
                     /locus_tag="RD743_000366"
     CDS             complement(30655..32178)
                     /locus_tag="RD743_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDU0562226.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(32171..33346)
                     /locus_tag="RD743_000367"
     CDS             complement(32171..33346)
                     /locus_tag="RD743_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDU0562227.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(33350..33535)
                     /locus_tag="RD743_000368"
     CDS             complement(33350..33535)
                     /locus_tag="RD743_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562228.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(33501..35195)
                     /locus_tag="RD743_000369"
     CDS             complement(33501..35195)
                     /locus_tag="RD743_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDU0562229.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(35192..35665)
                     /locus_tag="RD743_000370"
     CDS             complement(35192..35665)
                     /locus_tag="RD743_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDU0562230.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(35734..35889)
                     /locus_tag="RD743_000371"
     CDS             complement(35734..35889)
                     /locus_tag="RD743_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDU0562231.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(36023..36403)
                     /locus_tag="RD743_000372"
     CDS             complement(36023..36403)
                     /locus_tag="RD743_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDU0562232.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(36407..36622)
                     /locus_tag="RD743_000373"
     CDS             complement(36407..36622)
                     /locus_tag="RD743_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562233.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(36625..37032)
                     /locus_tag="RD743_000374"
     CDS             complement(36625..37032)
                     /locus_tag="RD743_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDU0562234.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(37298..38773)
                     /locus_tag="RD743_000375"
     CDS             complement(37298..38773)
                     /locus_tag="RD743_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDU0562235.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(38763..39611)
                     /locus_tag="RD743_000376"
     CDS             complement(38763..39611)
                     /locus_tag="RD743_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDU0562236.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(39648..40010)
                     /locus_tag="RD743_000377"
     CDS             complement(39648..40010)
                     /locus_tag="RD743_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562237.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(40011..40169)
                     /locus_tag="RD743_000378"
     CDS             complement(40011..40169)
                     /locus_tag="RD743_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562238.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(40310..40934)
                     /locus_tag="RD743_000379"
                     /pseudo
     CDS             complement(40310..40934)
                     /locus_tag="RD743_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(40931..41242)
                     /locus_tag="RD743_000380"
     CDS             complement(40931..41242)
                     /locus_tag="RD743_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562239.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(41286..41579)
                     /locus_tag="RD743_000381"
     CDS             complement(41286..41579)
                     /locus_tag="RD743_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562240.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            41774..42421
                     /locus_tag="RD743_000382"
     CDS             41774..42421
                     /locus_tag="RD743_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0562241.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            42482..43627
                     /locus_tag="RD743_000383"
     CDS             42482..43627
                     /locus_tag="RD743_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0562242.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(43708..44013)
                     /locus_tag="RD743_000384"
     CDS             complement(43708..44013)
                     /locus_tag="RD743_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDU0562243.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(44038..44469)
                     /gene="ruvX"
                     /locus_tag="RD743_000385"
     CDS             complement(44038..44469)
                     /gene="ruvX"
                     /locus_tag="RD743_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDU0562244.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(44466..44732)
                     /locus_tag="RD743_000386"
     CDS             complement(44466..44732)
                     /locus_tag="RD743_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDU0562245.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
BASE COUNT        12913 a         9326 c         7546 g        15113 t
ORIGIN      
        1 tttttctata ttttatgctt tttttcgttc atttctcata attgaaatcg attgtttcaa
       61 tgaactccat aatccgcctt cgctatacac gagtacattc gatttgtaca tgtttgcaga
      121 caagatcatc aataagaatg ttacggctac aagtaccaac aaggaaatca atgctccacc
      181 cataccgact gtctcatttg ctaagcgaac cggcataata tatgaagata agaatggaat
      241 atacgaagtg atccgaataa cgatattgtt tggatctgaa gctcctagta tcaatccaag
      301 catatatccg cctagagata gataagtcac tggcaaaatt gcttttgctg tatcttctgc
      361 tttgttgacc aaagagccac ataaagctgc taaaacagaa aaaatcaaaa tgccaaagat
      421 catgaataga agagaatacc acaagaatgg tccaatcaag ttatccaatg agataccgtc
      481 taatatattt ttgaccattt ccatatcttt gaattgactg tatcctatac caaaaataat
      541 accgtataat gccatttgcg ttaatgcaac aagtaaaaca cctgttagtt ttccgtaaaa
      601 atgcttttgt gcagtcgtac tggacaggat cacttccatg atccgcgtgc ctttttcaga
      661 agcgatctct tgtgcaatga tctgcgcata cgtcaaaata ataatgaaca agacaatcgt
      721 tgctacataa cttacgacat attggactgt cgagttatct tctccaactg tcatttttcc
      781 ttttgaatca aaattgactt tttgtcgagt caaacttgct tgttggctca agctagctac
      841 ttcttctggt gaaatgccta attgacttgc tcgaagcatc gattgcaagc cagtcagttg
      901 ttgttgtatc agtagttgtg tactttgtcc taatgaattt tcgctataaa gcatcccatt
      961 tatcttatca ttctcagttg agactactaa atacgcatcg attttttctt ccgaaagctc
     1021 tttttgtgct ttttcttcag aatcaactgc tttgaactca aaatcatctg ttttgacttg
     1081 gctcatttgc tgactgattt gctcattatc agccaatacg ccgattttat tgacatcact
     1141 attttgttga gcaaagtttc ctgctacata aatgataccc agcatgataa aagggactaa
     1201 tatcatgatc aagaaagaaa tcgacttaac attttttaaa tagacatctt tggtaataat
     1261 ccaaaactta cgcatcgatt tcacctgctt tcaatttgaa aatctcttcc aaagttggcg
     1321 gttgctggtt aaacatagga atgtagccga actgagtcgc acgagtaaag atttctttcc
     1381 cagcttcagg atcttctaag gtaatctcta acgtattgtc ttcatgaagg atcacttttt
     1441 tcacgccttc gatttcttct acttcctgtt ggcttaaacc agattccaag aataacttcg
     1501 tacggccaaa tgattcacga atctcatgta cttttccatt caaaaccatt tttccgtttc
     1561 gcaacatgat caaatgatcg cagatttttt ctacattatc catattgtgg ctagaaaaaa
     1621 tgacacatga accatgtttt ttcaattcca atattccatc ttttagcaat tcagcattta
     1681 ccggatctaa cccgctgaat ggctcatcca aaatgatcaa tttaggttca tggattaacg
     1741 tggcgatcag ctggactttt tgctgatttc ctttagacaa agattttact ttatccgtct
     1801 ttttcccttt tacctgaaat ttttccatcc aaaaatcgat ttttggttca atctcttttc
     1861 ttgatttccc tctaagttct gcaaaataaa gcagttggtc ttgaatcgtt actttcggat
     1921 aaagtccccg ttcctccgga agataaccga tgacatcata atctttctcc ccaagatgat
     1981 ggccattcca taacacttct ccttggtctt gtgtcaaaaa atccaagatc aaacgaaaag
     2041 tcgttgtctt cccagccccg ttttggccaa ttagccccat gatttttcca tcggggattt
     2101 ggaaagagac atgatcgaca gctgtgtagt tcccaaaagt tttaacaaga tccttgactt
     2161 ctaacatttc ttcactcctt cttcaagaaa tcacatatat ccttagacac cagacgtaag
     2221 cgatttatta tttaccaaca aaacgtaaac gctctttttg ttgtacataa aatgatagga
     2281 cactttactt tctttgtaaa gtgccctttc ctcattcctg atgtaattta tcaaatacgt
     2341 tttgtgcgac atttttcggc agtccgattt cttgtaattc ttctgtcgtg gctgctgtga
     2401 tatttttcaa cgatttgaac tctttaagca gcattttttt tcgttttggt cctaaccctt
     2461 caatttcatc taatcgggaa gcaaaactgt ttttactgcg tagctgccga tggaaagtga
     2521 ttgcgaatcg atggacttca tcttggattc gctggagtaa gaaaaactct ggtgaatttc
     2581 gctcaagcgg tactacagcc aaatctatac cgaatagcaa ctcatttgtt ttatgtttat
     2641 cgttttttgc catgccagca ataggaatat cgattcctaa ttgattttct aagacatctt
     2701 ttgccacatc tacttgtcct tttcctccat cgatcaagat caaatcaggt aaaggcaacc
     2761 cttcgcgaat cactcgtgaa tatctgcgat agatgacttc tctcattgat gcataatcat
     2821 ccggtccgtt gactgttttt attttatatt tccgatattc ttttcttgcc ggttttccat
     2881 caatgaatac gaccatcgca gccacaggat tcgtccccat gatattagag ttatcaaaag
     2941 actcgatacg tattggggtc gggatattca tcgcgtttcc taaacgttca actgctccga
     3001 tcgtccgttc ctgttttctg gcaatcagat caaaccgttc attcaaagca actgaggcat
     3061 tcttattcgc caattcaact aattttttct tttctcctcg ttttggttgc agcactttcg
     3121 tagctaacaa agcttctaca ctgggcttgt cgatcgtatc aggaatcaag acttctttag
     3181 gaatgaaatg ctcattttcc tgataaaact gaccgaggta agtcaagaaa tcttcttctg
     3241 cttcgttata gaaaggaaac attgaaacat ctcgttcaat tagctttcct tgtcttacga
     3301 aaaacacttg gacacacatc catcctttgt caatcgcata accaaataca tctcgatcca
     3361 gtaaatcagt atttgtcatt ttttggcgag tcatgattgt ctcaatggca cggatctggt
     3421 ctcggtattc tgcagctttt tcaaactcca tgttttcagc agctttttcc attttctgat
     3481 ggatctctga ctcgattttt tcgtgtcctc cgtttaaaaa ctttttcact tcatcaacga
     3541 ttttcgtata gactttcgga tctacgtcga aataataagg acacagacat tgtcccaaat
     3601 gatagtaaag acaaggctcc ttcgtctgac taggtttgca ttttctcaga ggaaataatc
     3661 gatcgaggat cttctttgtc tcattcgccg caccgacatc gggatatggt ccaaaataca
     3721 gtgctttgtc ttttagtact ttacgagtaa tcaccaaacg gggatatttt tcattcgtta
     3781 tcttaaggaa aggataggtt ttatcatcct ttaacataat gttgtattta ggatcatttt
     3841 tctttatcag attgatctct aagagcaatg cttcgatatt cgattctgta acgatgtatt
     3901 caaagtcttc gatttcacta acaagccgtt ccgttttcgt attatggctg ccagtaaaat
     3961 atgagcggac acgatttttt aatattttgg ctttgccgac ataaatgatc gttccatttt
     4021 tatctttcat caagtaacag ccgggctgat caggaagtaa agctaatttg tttttgattc
     4081 gttcgttcat ctggtactcc ccttcatatt caattaaagt tattatagca tgtctgtcaa
     4141 aaaaacaatc cagcctgaca acgtaattat actcaaaaaa tattaatttg aataacctta
     4201 actcaaccgc aaaaaaaccg aggacggaac tggattcagt cccaccctca gcttattttt
     4261 gctgtaagtt tttataaata ttgatcgatc atttgacgta gttgttcttt tgaatgaaca
     4321 ccaacagctt tttctactac ttgaccatct tttttcaaca ttaatgttgg aatgctcatg
     4381 ataccgaatt gttgaggagt ttctggattt tcatccacat ccattttagc aattctgaat
     4441 tcttcttcat catattcttg ttccaactga tccaaaatcg gtgcttgcat acgacatgga
     4501 ccacaccaag ttgcccaaaa atcaatcaat acaagtcctt tatctgtttc ttcattaaat
     4561 gtcttatctg taattacttg tgacatatca aagacctcca tttttaaaat ctatgattag
     4621 tatagcattt ctatacttgg atagcgaacc atctgcttac ctttagtaag ctttacttaa
     4681 atttaacaat tgtcgcacca tttccgcctt ggtttgccgg tgcaaactca aagcttttga
     4741 cgctccgatg gtttttaaga taatcagtaa ttcccttgcg aagtgcgcct gttccttttc
     4801 cgtggacaat agtcacttga ggataaccag ctagaatggc agaatccaaa tattgatcta
     4861 cctcacttag cgcttcttcg tatcgttttc ctcgaaggtc caattgattc ggcacgtgac
     4921 tactagattc agatcgtaca gcatggataa ctcgttgtgt cggctctttt tgaggagcaa
     4981 caggcgtcag ctcgtcttcg gacacattca tttttaatat accgatttct acttgccact
     5041 gattcccgtt tttacgtaaa agcgtccctc tttggccgta agtcgtaacc aacacttcgt
     5101 ctcccggttt caacgttttt tgttctttcg ccttcttcaa tactttattt ttcttcaaag
     5161 tttcttcttg atgtagattt gctaattggg tctttgcatc gatcaactga tgctctttca
     5221 catttccttg cccaatctgc tgttgcattt tacgaatatc agcaatgatt ttttctgctt
     5281 tttcttccgc ctctgacacg acttcgtttg cttttttctt tgctttttcc atttcttttt
     5341 cacgttcttc aaagaaatag ctgtaagctt ctttcaaatc atcgtgaagt tcttgtgcct
     5401 cagaaacaaa atgacgcatt tccagatatt cggtctctgc cattttccgg cgattctcta
     5461 aatctgtgat catttcgttt aaatcttggc tctcatcatt catcaactgc ttcgcttcat
     5521 caataacttc tgtatcaaga cctaatcggc tagagatttc aaacgcattg cttcgtccag
     5581 gaacgccaat cagcaaacga tacgttggac ttagtgtatc cacatcgaac tccatgctgg
     5641 cattaatcgt atttgcccga ttgtatccat aaactttcaa ttcaggataa tgcgttgttg
     5701 ccatcacata agcagacttt ttaccaagat catctaagat tgcgattgct agagcagcac
     5761 cttcttgcgg atctgttcct gcaccgagct cgtcaaataa taccagactt ttttcattga
     5821 cttttgataa aatatctaca gtgttcgtca tatgagaaga aaaggtcgat agactttgct
     5881 caatcgactg ttcgtctcct atatccgcaa atacttcctc aaaaataccg atttgacttt
     5941 cttcacctgc aggaatcggt aatcctgctt gccccatcag ttgtaacaga cctaaagtct
     6001 tcaacgtgat cgttttccca cctgtgttag gtcctgtaat gacgatggct tgatagtctt
     6061 ttccgattgt gatgtcatta gaaactactt tttcctgatc gatcagtgga tgtcttgctt
     6121 gtttcaagac gacatgattg tcttcactga ttcccggcac gatggctttt acttctttac
     6181 caaatcgtgc ttttgcattc atcagatcca ttttaccaat cacataagca ttgtggatga
     6241 tttcttgacg atggggaaca agttcagcag aaagttcact taaaatacgt tgaatctcat
     6301 ttcgttcagc gatttggtat tgacgcaaac gattattcaa atccaccact tgttttggtt
     6361 caataaataa agtctgtcct gaagcacttt gatcgtggac tacaccgcca aatacaccgc
     6421 ggtattcttg tttgactggg ataacgtagc ggtcatttcg catggtgatg atcgcatcac
     6481 ttaaatactt tgcatttttc ccacggacga tcccatctaa ctgttcacga acagcttgtt
     6541 cacttcgccg aatgttctgt cttatgctct ttaactcagg agaagcgtca tcagtcacgc
     6601 gtccgtcttc atcgattgct tcctttaaac ggcggctcaa aacagggatc gtgactaact
     6661 gatctgccca catatacaaa cgtgcaaatt cgatttcact gtcagaaaga tcatcaatga
     6721 aacgattcaa ttcgcttgtc gtagacaata ctctggaaac ttgtgcaagc tccactccat
     6781 tcaagtctgc tccgatctcg atccgtttca tatggggacg gatattttca agtttaggta
     6841 tagggatccc gcctcgcaga cgttgcactt tcaatccatc ctcagtttct tgaagccagt
     6901 ttgtgatcgt ttgtttctca ctcactggta ccaattctgc cagttcttct tttccttgtg
     6961 cagtcactac aaattgtctg accatctgtt tgactttttc aaattccaat gtttcaagga
     7021 ttcggttatt cattttatca ccttatcttt cactatgaaa aaagagctgt gccggacata
     7081 cgctacgcag ccttcttaaa taaactactc tgtacaaaaa acgaaatatt ttccagcact
     7141 attttcctta attcaaaata ttcgttacca aagctcatac atcttgttgg taagaaatgg
     7201 cgttctctca acgataaaac gagcgaaact atgctcgccg aattgatttt ggaccacatc
     7261 taatggaaca aaggcaagta actgcaaaat caaaaatata gtaacgtaag ccatgattag
     7321 tgacaggatg cctcctgcaa tccagtctac ctgtctcaag atcggcacat acgtcaatcc
     7381 atggacaaaa acccccacaa atcgggtaag cagccagcca attagcaaaa taatcagaaa
     7441 agcagttcct gcataaaaag cttcatctag gtgaaaagca aatacctgat caaaaaatgc
     7501 taactttgat gttggggtga cagctgggta tggaatgtat aattccaaat gacttgctaa
     7561 tggtttgtaa aaatgctggg cagcaataaa tgacaataca tatccaatcg cgaatatgat
     7621 ttgcaaagca aacccgcgac gtgctccaga aaaaaacgca attagcaata aaaataaaat
     7681 cagcaaactc agcatgctac gttcctttct cttctaactt ttttaaattt ttcttatttt
     7741 gataaaagga tcttaattag aagatttttg tttaatttgt ccttttcgtt tctcgtttaa
     7801 aatctgttgt gcttccacat gatttttaat ggtatgatct gcttgaccat tttctttcaa
     7861 tacttcccga gcttcgttct cgattgcttc aatgcgtttc acacgatttt caagctctgt
     7921 aaatttgatc atttttttct ttaattctgc tgtttcttct tccagctcca agattctttc
     7981 ttgtttcttc aattgatccg aaagcgcatt gacagccatc aagattgctg cttgttcatt
     8041 atccatttgt ggtgataatt gtttcaattc tgctaactgt tcattgatca gcttcgttac
     8101 gatatccata tgatgcttgg tttcccgtcc gataatagta tatgtttggt tggcaatcac
     8161 tgctttgtag cgtgtttttt cgtgcgccat acgaatcatt tcctcctatt tgatgaaagt
     8221 tttatttagt gtaccatgaa gttggttctt tcaccgttct ccacaaatat ttttcacata
     8281 cttaactatt atatgataaa ctttaaaaaa atgctatgtc tcgcaatctc ttagaaaacg
     8341 aaaagtcgtg agttgcggta tagttttatg tataaaaacg aggagaattt tatgagcaac
     8401 gtcattatca aagcaacaaa cgaccaactt agtcaaatga aagcatacta tcgctcttat
     8461 ttgttaaata aacagattcc ttatactagc tttgctgcaa aaaaagacgg gacgaccatt
     8521 accgcttata catccggaaa agtgatgttt caaggtacga atgcagcaat agaagctgat
     8581 aaatgggggg aggcacagga taaacaagcc aagccagcaa aaacagcctc ttctttgcca
     8641 aaaaatatca gtcaattatc cgttttaggt agtgatgagg tcggaaatgg cagctatttt
     8701 ggtccgctaa cagtatgtgc tgcttatgtg aaacgtgatc agcttgcaga attacgtaaa
     8761 cttggtgtta gagattcaaa agagttaaaa gatagccaaa tcatccagct tgctgacatc
     8821 ttaaaaaaaa ccatccccta taaactgctt gtcttatctc cagaaaaata caatgagatc
     8881 caaccaaaat acaatgccgt tcggatgaaa gtcgctttgc acaatcaagc gatccatttg
     8941 ctgctaaaaa aaatcgagcc agaaaaacca gaagcgattt tgatcgatca gtttacgccg
     9001 gaagcaaatt ttaaaaaata tgcgcgttta gaaaaaaatc aattacaaga aaaactgtac
     9061 ttcgttacta aaggagaaca atatcattta gctgttgctg ctgcttctat catcagtcgt
     9121 gccagctttt tggaagagtt acacaaagca tcagctgaag ctggaatcac ccttccatca
     9181 ggagctggaa cgaaatctga ccaagtagcg gcaaaactat tggagaaagg cggcatgcct
     9241 atgcttgaaa aatatgccaa acttcatttt gccaatacag aaaaagcttt gaagctgcta
     9301 aaaaaataat ggaactgtga caaaagtcat gtcacagtcc cttgttccga ataaacggtg
     9361 ttctcatgcc aactcctcgg tcacaagtca caatagtcat caaacatgag aagctgtttt
     9421 ttgaaattgc tccttgtgac gcggagttaa acggcaggct cacaacctct tccttaaacg
     9481 gtgttcaaaa gcttgcatct gcgataataa gcccggccaa acgaaaaata tgaggagcta
     9541 tttttgtttg gctgggctta tttattactg aaaataagtg gtcaatgctt gtgtcaaact
     9601 tgcagcaatc tgctgctgat aactgtctgt tactaattcc tttaaatcac tatcattatt
     9661 catataaccc atctcgatta aaatagaagg ttgaagattt tctcgaagaa cttgatagtt
     9721 cccagtggag attcctcttg aatccagagg cagatcagtg aaggagtcag caattgtctg
     9781 tgctaatgtt tcctcttgtc catagtaata atatgtcgtg attccagttg cttcattcgc
     9841 atattccgta gagtctgcgt gtaaactgat aaagacatct gccttttttt cttcactgta
     9901 gtcgcatata tctccaagct gtactaattc atctgtagta tgggtcaaat aaaccgttgc
     9961 tccagcatcc tctagcgcct tttttacttt tatggctgta cttaatgtta tatctttttc
    10021 ttctgtttgt tcatcattgc ttaatgcccc agtatcattt ccgccatgcc caggatctaa
    10081 tacaatcact gcatcagata gctcttccgg ttttgtttgt atattctttg tgactttggt
    10141 tccatcttcg agggctaaat atccagtata gccatcattt gtcttgatct gataagcatt
    10201 ttttgtttca gaaagaaact ccgccagctc tccgccgttt atagttgcaa gtggggtagt
    10261 tagcgagtca tctgtatata gtgtacggtc attttttccg attttgatta atgtctcttg
    10321 ttctgtatca tcaaatgatt cctttgtctt tgtaaccgta ctgttcgtac tgctgaccgt
    10381 ttgtccaact atcttttttt gaccaaaaac atctatttga aaacagataa taatgataat
    10441 gattgcaaat acaaaagctc caattatttt tttcattctt gactctcacc tcgtagatcc
    10501 agactaaggg tcaaaactta aaatcagctt aagtacgcaa aaaaggggct gtaaaagata
    10561 taaccgtaga aaataacatt actgctaaaa tgattttttt catttttggc atatcctgaa
    10621 atctcctttt gactattcgt ggtgattcat ctacccaata aatagtttcc ttcattctac
    10681 aaacaattaa tttcttcgtt attaaattaa aacacttcct ggaacaccga cataataaat
    10741 aaaaatatac cctgataatc atgagagaat aacaaatttg tatttatgaa aaactaccta
    10801 tataaaaaaa acaagatata acgatcttcg tcatatcttg cttcttcacc taactatttt
    10861 tctccctata taacagcttt acgcttctcc taatacacca ttcaaatata aatcgatcaa
    10921 ttgcgccaca tttagcggtt cttccacagg tcttttcgac cgagtgatgt aaggtgctaa
    10981 cactaagaaa aaatgccttg ctgcgatttc ttttgaaatc ccatttctta gcacagattg
    11041 gtttgctgaa aagatttctt caaaaggttc acgataattt ttcttccata gaaagaaaag
    11101 atgatctctt gtttcctttg aaagattgct ttcaagatcg tgaagcatca gaaacaaatc
    11161 gaccagatgt gtctcaatca aaaactgcgc catttttgtc aaggttactt gaaatcccgt
    11221 ttcgcttgtt tgaacgattt ctctcaaagc ttttccaaca tcttttaagt ggttttctaa
    11281 aactgccgta tatagttttt ctttatcacc gaaataatga tagagattgg gttgggtgat
    11341 cccaactttt tgtgcgatct ctcttgttga agtgccgaga tatcccttgg acataaacaa
    11401 gtcagtcgct tctttcaaaa ttttttgctg tgtatctttg ttgtatcgat ggaccatttt
    11461 tccacctgct ttttggttat tctttttctt tcaatacacc atcttccatc ataaatattc
    11521 tatcgcattt gtcaatcagc cgtgtatcgt gagtaaccat gatcgttgct ttatttttgt
    11581 cttttgattc ttttgccaag atatcgacta cttctcgtgc cttttctgaa tccaaactag
    11641 cagttggttc atctgctaga ataatggacg gatcattgta caatgcacga gcgattgcga
    11701 ccctttgccg ttctccacca gatagttctt ctggatactt gttgatcaac ttatccacgc
    11761 caagctgctc aaataattct tttgcttctt tttgacgatg ttcatgtttg atcttatcta
    11821 ctaataataa ctgctttttt actgtcagaa aaggaaccag attggaagct tgcaaaataa
    11881 agccaatttc tttaaaacgt agtttaacgc gttcactttc tttttgatta ctgaattctt
    11941 gatcattgat aaatacagag ccactgctag gcgtctgcaa tccgcctgcc aaagtcaaaa
    12001 aagtactttt acctgaacca gaaggaccga tgattgcaac aaattcccct ttatcgatac
    12061 tcatggttgt ttctttcaat gctttaacta cttggtcgcc atcttggaaa cttttttcta
    12121 cttctttaaa ttctattgct ttcatgctct tcctccttct tatccaattg ccttcaacgg
    12181 atcgattttt acgattgtcc gaacagaaaa tagtgcgcct aacacagcta ctaccagcat
    12241 caacaagcta atgacgccaa aaaacagcca attgctttgg aatggaaccg catcaggtag
    12301 aacaagggat gttcccactg ttcctaatag accaagaaga ataccaacaa atgataaaat
    12361 gaatgtttgt gccactacag aacgcgcaat aaatcctcct gtgatccctt gtgctttcat
    12421 tacaccaaag atattaatct tttgcatcgt cagtacataa atgaaaatcc cgatcacaat
    12481 agccgcaata acgatcaaaa atccgatcat aaatccaaaa gtcaatactt gcgcattata
    12541 tccaggtaat tcattgatga atttagaaat tttgatctgt tctaaatctt caggtaaatt
    12601 actgatttta cctctgacaa ctattgcgtt aatccgtgca ttttcacttg tatcttcttt
    12661 ttcgaagcgg atctcttggt aagcattgat ggttgtatag agaactggtg agacattgaa
    12721 tttggcatga tccgtaaagc cagttagttt aaacgtcttg tcactaccag ataacttaac
    12781 tgtatctcct acggctagac catattcttc tttcaaacta atatcgccta ctgcctcatc
    12841 atcgttatca aaagcttttc cttcaacaag attgggcatg atgaattgat ttttatcgat
    12901 accgaagaag ctaacattga tttttccttc ttctgtactg tctttactag tcacgactcc
    12961 aggtgtctgg gcaagatacg ccactttccc accttcgact tcttctgcta tcttcttagt
    13021 aatcatggac atccctagat ttgaatttgc atctttcgaa agcacaatcg aatctgcctc
    13081 ccacttgtcg actgctgttc gattatcttg agctaagcca taagctaacc cagtcaaaaa
    13141 aaataccagg taggcgatca aaaacatcac ccctgcaact aacgcataac gtaactttga
    13201 atgcataatt tcatttaatg ctaaaaacat ttaactcctc ctcctgaaat aatcatttta
    13261 tacaacaatt ttatcacttg ataaattttt ggcgaatatt atgctcaaaa aattcttccg
    13321 ctatttcatt atatgaattt tttattctcg attgattaat atttattata tccccacaca
    13381 ataaaagacc gtgatatttc tcacagtctt ttatctactc tattgctatt tgctctctta
    13441 gctagtactt taaaagctaa ctcattgagg tagtgaaaac gagaggtgtg ttcagacaga
    13501 aatccaaaat cctcaactgc tagctgtgga atcgtagttt tgtgtttcac gatatccgca
    13561 agcaggttca aatctgataa taagaattct tggcttatca gctgtccgcc aattatttct
    13621 aatgtatccg gatggtagat cagctttgca tttactgttt tgttttcttc atcgtattgt
    13681 ggaaatagat caaattctcc tgaacacgaa ccggtatttt gttccaagaa tggcgcttca
    13741 tcttctgtca gccctacagt tcctaaaaag tagtctataa tgactgctgc tgttatcttt
    13801 tgtctgaggt cgtaagcggt tggctgacca ctcaaagtta gcgctactgc tcttgccata
    13861 tgtacagctc gagtaactaa aggtaaaaag agatgccgtt tatttttgtc aaaactgact
    13921 gggacgatat ctccaatcgc atagatgtcc ggatcacttg tctgcagata ttcattgact
    13981 atcacagtct catcctcata caaagccact ttttctttta acaatgtcgt attaggacga
    14041 gtttgggcag aaaaaatgac catatctgcc tttattgctt gagatagaag ttgtacggat
    14101 actactttct cttctgattt tttccaatcg atcaagaatt catttggata aaaattgatt
    14161 tgcttagttt gcagctgatt ttgaaaactg ttgatcatat ccttatcgaa atatcttgat
    14221 aaaacattcg gcatataatc aactagatga ataatgtatc ctttatttct caaagcatct
    14281 gctaattcca atccaatata accagcaccg aagatcacta cttcttttgc ttcttttaaa
    14341 gcggcaagta cttgcttaga ttgcactaga tttttatagg tgtagacatt ttcagcttca
    14401 atttgtttat acaaggtgac aggagaggaa cctgttgcta cgattaattt atcataggtt
    14461 atggtctcgt tttttccttc tttggatgcg aggatgagct gatgtttgtc tggatcaagt
    14521 tcagttactc tccattcggt gatcagtttt gccccacttt cttgaagagt ccgcatgtta
    14581 tgggcaactt cagctaaact acgtatcttt ccttttaaat aaaggttgat tccatttgaa
    14641 acatagccta gtccatcttt atgatcttca tcgatcagta aaacttctgc tttcggttga
    14701 agttttctta gatttagggc agctgtgatg cctgcatggg aagcaccaat gatcacgatt
    14761 ttcatgtttt attcatcctt aaattcaatt tttctaaata acattctttt aaaaattggt
    14821 tcaaataggg gcaatcataa ttatattcgt tgctagtgaa tacaaaaact ttagcggact
    14881 tctgtgacgg gtactctttc aattggctag tcaaaatgat atcatactgc ttttctttct
    14941 cgtattcgac taaatcatat ttgatcgtat tcagcaattg ttcctggatg atttctttca
    15001 cgatatccgc cattagcctt tcataaggaa gatcataagc aatttgcaga cgtaattcta
    15061 actgcttgat cagcgtccgc aaaatagatg catagcgagc aaataattca aagcgggcat
    15121 tttcctcgtc tacttcagta aaatcaacaa tagcaaatgt ttgtctgacc aatctttcac
    15181 ataaaaccag gaattgttta tcttctactt cagttaaccg ctgttttaat ccttcttggc
    15241 caaaaatatt catccatcct ctaaaatagg caaaatgagc gtgattttgc atcactgtca
    15301 acgcaatctt ttgcgaaatc tcttctggaa aaacataaaa agagaaatat tcttctatcg
    15361 attgtttaaa cgttcgattc aattgagcta ctacgtcgtc tttgtatttt tcgcttatca
    15421 aaaactcctg gacatgcgag ttatgtgtat ctaatgtaaa gttactgatc atgaaaagat
    15481 aaaaaaccaa cgtttcattt tgattccaag gaaaggcata ttgaaagaag taacgggtca
    15541 aaaatgtatg gaggtccaat aaaaattgtg tcgtttgaaa caatccaaca ggaagattgc
    15601 tataatctgg tttggatttt ttcgtacagc gcattcgctt cttcataatg gaaagccata
    15661 tttcaaattt caatcgtcct tcttctgtaa tagtaaaact aaactctttt tgcagcagat
    15721 ctaaaaaagt gtttcctgat aaatcactgt attctttttt gttttcaaga taaggacgtc
    15781 caaaccaaaa taattggaaa taaaaataac aaatttgact ttctggaccg gtcagccgcc
    15841 cgctttttat tttcagatga aactcattta aagaatcatt taattccttc actctgcgag
    15901 acaaagtagc cgaactgata ttaagttcca tgacaagttt ctgccgatca aatgagccat
    15961 tttccaacag ataaagaagt atctgatatt ttgtagaata gcgcaagtag caatgataat
    16021 aaacatcgac tgctgctata tttccatgtc tgattaataa aatattccct tcttggataa
    16081 caatttccaa ttgtttgata ttttccaagt tagcttgtaa ataaaacaga tcttctttca
    16141 aggtagcccg atcaactgat aagcgggtaa gtacgttatt aacggaaatc tctccttttt
    16201 cctctaataa aagttggaaa agtttgattt gccgttgttc atttttttca agcaaactga
    16261 aaagttccaa gtccactcct ccaagcattt ctgattttaa cagcagaagt ttccttctca
    16321 tccttgaaaa agaggatctg tgacagttat cacagatcca tacaaattct aatcacttag
    16381 ctggttggcc cagctgtcag ttcccatgtg accatgcctt gatacgttgc gccaggttga
    16441 gcattcgtac tgctatttac ctctaagagt aaaccatcgg tatcgggaaa gtcaacgaga
    16501 acctctcctt tagctgttcc atcactctca taaatcaacg tgttttgacc ttgctgaatc
    16561 aattgttttg atccagaatg attgaagtat aattttgttc cgcttaattc ttgaccttga
    16621 tcattcttaa ataaagaagt caatgtccct gtcagttgcc aaggagacgt gcttggaagc
    16681 cgagtatccc gcacatcaaa agaaaaatcg ccttttgctt cataataact tgtcttcaac
    16741 ggtttgggga gatcgttacc aaatgtaaaa tcatctggta cattaactaa tgttaattct
    16801 tgtttgggga caagaattgt tttagcagat gtttggacaa ctgggttagg aagcttagca
    16861 ccatctgcta atgtaatatt gctggaaagt acagctgcct ttgtcagtat gctgacggat
    16921 tgtacagaag tatctacagt atttaattga tagttgtaag taagatgaat ggtatctcct
    16981 attttgctca atagatctgc tgggacagaa actccttgtt gaatgccggt atctgcgata
    17041 gttgtaccat ttaattttaa ggaaccattg atataagtag tattttcagg aagtgagatc
    17101 gtgatttttt tgtcggttat cgcatcccca gattttgtta acgtgaatgt cacattttct
    17161 tgaatcgtat ctcctgatag agcagacagg ggatctcctg tctcaggttg tctgctaagg
    17221 ttttttagct gactatctgc actcacagat ggaaaattag ccactgttat ttgaggaaga
    17281 gtggtgattc cagcttgttc agactgccat ttgtacgcaa aagacggata catgttagta
    17341 agtacatcgt ccaccaagcg ataatcaaaa taatattgcc ctgaagccaa tgcaggtagt
    17401 gtagaagtag ctgtatttac acccccgtta tttgattgga tcgtttggaa aagttgatct
    17461 gtccatcctg tcgctaattt ttgggttcct ttcgaataaa gcctgtaatg gatcgaaccc
    17521 ttttttagtt cagttgctga ccaattggcc gtgacttggt acgatgcaga agaaaggagc
    17581 tttccccact gactatcggc tgttaagttt gttggtaaaa ccgttccgtc tagcttgtac
    17641 tgctccaata cctttaaagc aacaggagaa agcaatccgc cttgcgcatc tttcccactg
    17701 tacacgatat tggctgtctg attcaatcca cttgtgcttg ctggagtatg agtcagtatt
    17761 gctccttgtg taacgttttt tcctgtaaaa tagttgacta cgcgatcaaa gcgactaaat
    17821 ggcaactgat caaaacgtcc ttcagtcagc cccatcgtca atgtccgact attggtagag
    17881 cctggaaggt tctggttatt tccatccacg tattctgtgg tatcagcaaa tgtattatat
    17941 tttcctgttg aatcacttgt tgagatgtaa ctattccacg tatttatctc tgcacttgca
    18001 gaaaatgcat atgctcctgg attcaagttt ccgccagtta atttaggata aaatctacct
    18061 gcagctggag ctaaaatcga attgaacata cgatcttgga taatattatt cgaatcataa
    18121 aaatcaacag aaaaaacggc ctttttagac atagaaggtt gtaatttacc cttggtcaaa
    18181 attttgtttg gactcgtatc atatttatct ccgttttctg tcatcttcgc gacattagct
    18241 gtaacatcaa attgaagcgg gatatcgtcc acgttgaatt cgcctcgcgt aataatagca
    18301 ataatatctg ccaatagagt atttgtatcc cacgacccca tcccaaaacg caaggtatgg
    18361 cttcctgctg ataataaact accgctagta accttgatgg gggcaccggc aatatacaag
    18421 taggcgttgt tccagtcaat tgcttttaat acatcactgc ttgatacatg ttccggcaaa
    18481 ttgatgatcg ccacaaatgc aaatccttgt gcagtgatat tcaccaaatc aagtacattt
    18541 gttagtttca catggctttc ccaatgaaaa cggaccgtgc cacctttcca aggatcattt
    18601 gaaccatcgt tcaaataagt agcagtcaat ttaggcaatt ggttagcccc agttagttgg
    18661 acaagcttcc cgccattgac tgcactacgt ccaccatttg gaggtacgat attttcatac
    18721 gtgtatggat cataagaaat ggcaggaggc gttgctccaa cagcttctgc cgaaggtgaa
    18781 aagataaata tacttaaaac aaaaagcgaa gtaaaaaaga aggagcgaaa aaatgaaaat
    18841 ttattttgta gcaacatgtt catcctcctt ttcctttctt ctccataaca acaaccacgc
    18901 aagaagccat aattctatac caatgatcaa ataaagaaaa cggatctttt cttcattcgt
    18961 ctccgggaat acgccatgtt gattagattc tttataaatc ggtagctgtg tctgctgttt
    19021 tgatgcgcca ttgactgaac cacttggatg cggaactgga cttagatcat aaaatgtaat
    19081 cgtacctgaa cttttggtat ctgcttggac tggctcttgc cagctgaaga acagcaaact
    19141 tgcaataatc cctcccaata agatcgatgt tttcaacgaa atcacctcat catgttgttt
    19201 ctcctttttt ctttttcttg tggacccaca aaaacagcaa aatcaaccat aacactagaa
    19261 ctgcagcaac caatcctgca taaagccatg ggaaagattg ttcttgtact gcaaaaacgg
    19321 aattttcatt tactttttgt gctttttctt ttttgatcgt aaagttttgt gtaaatcgcc
    19381 attcattact gtcgcctgat tttgctatga tttctgcgcg gtaatcgccg gcttttgctt
    19441 tatcaccagc taaatcaaga ttcaagtgaa aattcgaatt tggtgcaact aaaaattcat
    19501 tagacgtatt ttcaatataa agctgatttt cataaaagat cttgctcgta aatatcaaat
    19561 tgctgataat cctaggctga ggattttgga aacgaagctg aacagttggc tttgcgttga
    19621 cctcgtccaa atctcctgat aaaagtttca gatccggttc tatcgtctct tgactttctt
    19681 gtaaaacgac tgcgagatta taagcaatac gatttttgat ttgtgctcct tctgtagcat
    19741 tttctgtttc agcttcttct ttaggtgaaa catgaatacc cgctaataca cgtcccttaa
    19801 agctttttgc tgggattttc acttctatag gtatagttgt ttctgcatgt gctgatacat
    19861 tgatttcctt tttcgcaatg agcaaaacgt cacggacatc aaacggcaat gtctcatcta
    19921 acgttgggtt tttttgacta taatcgatcg ttccaccatc actcgttacc gccggattga
    19981 cagagacctc aaatgttcgt tcttcatctg aagaattgct gataattact tctagtgtct
    20041 gattttgttc cggtgccact aataaatcaa aataaccgat tgctttatct atttggttct
    20101 ctggaaatac cggtttgatt ccaaagtctc ctgctgaatc ctttgcttgg ctgattgacg
    20161 gaaaaatgaa aaatagactt aaaatagaaa aaaacgaaac aaacagtcgt ttactttgat
    20221 tcattgtcca ccacatcctt tcaacaacaa ctgcggtaaa aaatccttca cctatagtat
    20281 tttcaccgca gtttgttgaa tttatttcat actctatgct actgggccag ccacgaggtt
    20341 ccaagtcaaa tcagccgtga atgttccagc tgctgctttc ggcactttca gtgtgatatt
    20401 tgctggatca taaacagaaa gccatgttcc cagaccttga tcttttgctg ctgtaaatat
    20461 tggtgtcgct gcacttgctt tactcaaccc agttacagtt tgactagttg gtccagtgga
    20521 ttcgttatct gacgtacttt taacagtgga tgttccaaaa tctaattcag cgccttgcaa
    20581 aacttgtgaa ccgtttacaa aatctgaaac cgaaactgtc acgttccacc cttgtgcttg
    20641 gccatctgca cctacacctc gacgatctga tacttggaga taaggtgtat cgttttttga
    20701 agcgtctact tgatacgttt ttgttccgct ttcaatctcg tgtgtaccaa atggcaattc
    20761 aggaactaca tccaatgtta aagaacctgt ctggccagtt gctggatttt ccggatctgc
    20821 tggctctact ggttttgaag gatcagtagg gttaagaggc tcaacggtat ttgttggttc
    20881 agtaaaggta atatctcctt ttgaagtcgc ttgaccctca gcaaaagctg gtgttgccaa
    20941 gccgcctaga agaagtgttg aaagtaataa tgtcccaatc atgttctttt tcataataaa
    21001 tcactccttt ttcttagtga cctaagtata gtagagacag tatgcaaaaa atcctcatga
    21061 attcatccgc ttacttgaaa ttatgaccct ttttgtataa aagagaaaaa aacaataaaa
    21121 tcgtttacat gaatattctt ccttttttat taagtatgaa aaaatatttc aattttattt
    21181 tttcgtttca aattattcta ttagtgacat ccttgaacta taaaaatagg agtacggcat
    21241 catctgccgc actcctattt ttttcgtcat ttatttctta aataaaacga ttctcacgca
    21301 attttatttt tttgatatct tctactgttt gtgttccagc taattgcatg accatttcca
    21361 attctgtttt aaagaaatca aacacttgat ggactcctgt acttcctcct aaagacaatc
    21421 cataaataac gggtctgcca atagcaacaa gatcggcacc tgatgcgatt gctttgaaaa
    21481 catgttgtcc acgtcgtaca ccgctgtcaa acacgattgg tacacgttta tctactgctt
    21541 ctgccacata ttgcaacgaa tcaaacgcag caggcccccc atctaattga cggccaccat
    21601 ggttagatac ccaaattcca ccagcaccag attctaatga tcgatagaca tcttcttctg
    21661 attgaacacc tttcacgtag actggcaaat cagaatgagc agcgataaat tctacatctt
    21721 ttggactaag tttttgtttg gaagatttat atacagcatc catcgtttgg cctactcctg
    21781 attgataagc ctgaacgatc ggcattggca gcgggaaggt aaacccgttt ctgcggtctg
    21841 tttcacggtt tccgccaact gttgcatccg ctgtcaatac gattgctttt gctccattac
    21901 gcttggccac ttcaagaata tccaaattga ttccattatc tttgctcata taaaattgaa
    21961 accattgcgg tgctttctcc ccgcctgctt ctcttatctc ttctaaagta caagaagcgt
    22021 aagaacttgc tgtatagatc gtcccaaaat ctgctacacc ttttgcagaa gctttctccg
    22081 ccttcacatg agctaatcca tgagcagcaa ctggtgccat gatgattggt gcagtcagca
    22141 tttcttcgtc aaaatgtgta gttgtatcgg gtaattctac atctcttaat acatgaggaa
    22201 taatcaaacg atgattgaat gcccgttcat tttcttgata agtaaataag tcgccagcac
    22261 cactgctgat gtaaccataa ccaccagctg gaatcacttg tgaagccgcg atttctaaat
    22321 cttccatatt gataaaatct actattcctt catttgtacc tgcttgatat gttttttcca
    22381 tctcaatgcc ttctctctat aaaatttgaa tgatgaacaa tatgatagcg tttacacttt
    22441 ttatattact cttcgacatt gatacaggca aataatctgt ttacatttca gccattccag
    22501 atgtcactac tctggaagaa atgattgtaa taaaaataga taaaaaaccg atgggacttt
    22561 gacaaaagta gtgtcaaagt cccttttttt gaataaacgg tgttcaaaag ccattttctc
    22621 ggtattaagt caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttact
    22681 actcgaaact aagcgacttt ttcacaacct ctcgataaaa cggtgttcaa tcagctgacc
    22741 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
    22801 atactcaggt cagaacgttg acttcacagt ctcttcctta aacggtgttc aaaaacttgc
    22861 atctgcggta ataagcccaa ccaaacgaaa aataggagga gctatttttg tttggctgtt
    22921 cttattactt gatgcagggc aggtttttca cgacctctga ttcacggtgt ttctttttca
    22981 tcaacatttt tacgaaatac tgtggaagac tattgaaata agtgtggatt gtttctttgt
    23041 gtttcaatcg tttcttgcca ttcttcattt aattttggat taccgttttc ttccttgaac
    23101 tcataaggtc ctaattcaaa aatcccagac agtatttgat tcacgatttc atatagttca
    23161 gtgtgatcag acacatgatt cttgaagatc atctcattat caccacgttt gaacctcaca
    23221 ttcgggctct catgatgaac ctctatgcat ttttctcctt tttttaactg acggacttct
    23281 gaaaacatct cataaatatc ttctccagta aaaccttcag caagcgccag actgtccaca
    23341 agaccgccta ctgtcagttc agagattatt gcttgattgg aatctaattt taatgtatag
    23401 cttgtcgctt ctactacgcc acatgcattg caaccgcctt ctgatagttc gctttttcta
    23461 atcaattgaa cttctttcat tttctcttcc tcctcttaat tttctactta tgtattttta
    23521 tcacctgaca taggtgactc ctatcaacgc tatcatgtgc ttttttcctc agaaatccta
    23581 tttatccatt ccatctatcc cttattcttc tacttttctt tctctagcat ataagagata
    23641 cgcatagtat tcaagcaagg attgtcgata taaaaagaac aaatactgct attttgattt
    23701 cttttggcta tattacatat aaataaaatt tatcatacct gctctttttg actatatcat
    23761 catctgactt ctatacttcg tttgtattaa aacttaggag gaatcattca tgcttacagg
    23821 gttaaactta ttaaataatc cttttctaaa taaaggaaca gcttttacaa aagaagaacg
    23881 ggcaaaatat ggaatcactg gtatgcttcc aagtactgtc caaacactgg aacaacaatc
    23941 tgttcaagct tatggtcaat acttaagcaa acaaacagat ttggaaaaac gtatcttctt
    24001 aatgaatcta ttcaatacga atcggacatt gttttataaa ttgatgggcg aacatttagt
    24061 tgaattcatg cctgtcgttt atgatcctgt cgttgcggat tcaatcgaac aatacaacga
    24121 aattttcttg aaaccgcaag atgctgcatt tctttcaatc gacgaaccag aaaacatcaa
    24181 agcaagtttg aagaacgcgg cagatggacg agacattcgt ttgattgttg tgactgatgc
    24241 agaaggaatc ttaggtatgg gcgactgggg tgtgaacggt gttgacatcg ctatcggcaa
    24301 attgatggtc tatactgctg ctgctggtat taatccagca caagttttgc ctgtatcgat
    24361 cgacgctggt actaataatg aagaattatt gaataatccc ttatacttag gcaatcgtca
    24421 tgctcgtgta gaaggcgaaa cttactatga attcatcgat caattcgttc aatcagctac
    24481 cgaattattc ccagaattgt tattgcattg ggaagatttt ggacgaggaa atgctgcagc
    24541 gatcttagaa aaatacgaag atcaaatcac tactttcaac gacgatatcc aaggtacagg
    24601 aattgttgtc ctagcaggtg tattaggcgg attgaatata tccggtgaac aattgaaaga
    24661 tcaaataatt ttgacatttg gtgctggtac tgctggtgtg ggtatcgcaa acatcctttt
    24721 agatgagatg atccgtcaag gtgtacctga agaagaagca cgccaacatt tctatcaagt
    24781 agataaacaa ggtttattgt ttgaagatac agaaggattg actcctggtc aaatcccatt
    24841 cgctcgcaaa cgctcagatt tcactaatgc tgatgaattg acaaacttag aagcagttgt
    24901 aaaagcgatc catccgacaa tcatgattgg tacttctacc caacctggag cattcactga
    24961 agaaatcgtg aaagaaatgg ctgctcatac accacgtccg atcatcatgc ctttgtctaa
    25021 tccaacaaaa ttggcagaag caaaagcaaa agatttaata gaatggacgg acggaaaagc
    25081 attagttgga acaggtatcc ctgctgctga tgttgaatat aatggtgtaa cttaccaaat
    25141 cggtcaagca aataatgctt tgatgtaccc aggtttagga cttggtttga ttgcttcaac
    25201 agctacacga gtaaatgctg aaatcatttc tcaagctagt cgagcattag gcggaatcgt
    25261 tgatgtaaca aaaccaggag cagctatctt gcctccagta gcaaaaatca ctgaattttc
    25321 tcaaacgatt gctgaaactg tcgcaaaatc agttgtcgca caaaacttga atcgtgaaga
    25381 aatcacggat atcaaagaag cagttgaatc tgctaaatgg gttcctgaat ataaatcttt
    25441 agaagattaa gatcgttata gtaaaagaac atacagaaac tttgtatgac gtctaaaaag
    25501 aaaaaagcga ctgcggcaaa acagcggcca gccaactcct gctcttttgt cgcttttctt
    25561 tggctttttt catatgatac agaataagaa aggatctgta aaaatggcac aaaaaacaac
    25621 aactgcaact gcagttgagg aaaagaaaaa agaaggtttt tggtccacaa aaatcaccgg
    25681 cgtaagtttg ccactttatt tgatcatggt ccttgtcctg atcattgcaa tggctttagg
    25741 gaaattaccg acgaatatga ttggaccaat cgcagttttg gttattttcg gtaatttatt
    25801 tcattttatc gggacaaaaa tccctatcgt caaaagttat ttgggtggtg gttccgtttt
    25861 tgcgatcttc gcttctgctg cgattgctac atttgggatc ttacctgaat atgtagtgaa
    25921 atccacagaa aactttgtca gcaatatggg cttttgggac ttttatattg ctgccttgat
    25981 tactggtagc atccttggta tgaaccgtaa tctattgatg aaagcttctg ttcgttttat
    26041 tcccgttgca cttatttcaa tggtcgtctc cttttttgcc gtaggactcg tgggcatgct
    26101 tatcggaaat ggttttgcaa attccgtttt atacatctca ttacctgcga tggctggtgg
    26161 tgttggagcc ggagccgttc ctttatcaac gatttatgca aatgttttag gtacggatgc
    26221 ttcttctatc atttcccgcc tgattcctgc gacagcaatg acaaatgtct tagctatcat
    26281 cggtgcagca ttagttgctc gtgctggaca atcaatgcca aaattcaacg gcaatggtaa
    26341 attaatggaa aaaggcgatc ttggcgatgg aaaacctcga actgtgaaac cttccattcc
    26401 acagttaggt gttggtttga tggtatcatt gactttcttc atcttaggac aaattggtaa
    26461 ctattttgtt cctaaagtcc atgcttacgc atttatgatt attatcgttg tcatctgtaa
    26521 aatcgccaac ctattaccag agtattatga agatgcagct atcatgttca ataacttgat
    26581 tgtcaaaaac ttgacagcag ctgttcttgc cggtatcggt attgcgttgc tgaatctgaa
    26641 tgtattagct tctgctttga catggcaatt tgttgtctta tgtctgacaa gtgttatcgc
    26701 gatttcagtt gtttcaggat ttgtcggtcg tttgtttggt ttatatccaa ttgaatcaac
    26761 tattactgct ggcttatgca acaactcgat gggcggaaca ggaaatgtag ccgtcttatc
    26821 tgcagctaat cggatggaac ttattgcttt tgctcaaatg ggcaatcgac ttggtggtgc
    26881 catcgttttg atcatctctg gtttcttgat gcaattactt tcttagtcaa aaaaacaaaa
    26941 atacctgatc atcaagtatc ggcttggtga tcgggtattt ttttgataaa gatttacagg
    27001 attttcaaaa atcttttttg cattttatcg gaatgataac cgatgtgttc ataaaattgg
    27061 tgtgcttcta ctctgtattc tgcagaatta agccttattg tcgttattcc tcgttttttc
    27121 gcttcttctt ctacgccagt catcaaagcc tttccgatgc ctcttttttc agcatattta
    27181 gacacagcaa gtgccataat attgaacatt ggatcgctaa aaatcgattc ataggactcc
    27241 gcttgtacat agccagctgc ttttttagaa gtaggatctt cataaaccaa gaaaaaattt
    27301 ttctctggct ccttcagcag tttcttcatt tgtctcaccg taacggttaa tggtatatca
    27361 taccctaatt gatccgcatt gatttcacgt agtgcttctg catcttctaa acgcatttcc
    27421 cgtaacatcc aaatcaccag ccaatttctg attttttaaa atgaattttt ttatcatagt
    27481 atactctttt tttcatttag aacaatatgt ttcagtgatt ttgacttgcg ttagtctaga
    27541 ttctcctaat gtttgtaaga tgacaaaggc attgtaacaa ttgaccaaaa gaaccacgag
    27601 gacaattact tgaaaaataa aggtcacttt ttttggagac aaaaccctgc taaatcttgc
    27661 tccccacaaa cctccaataa cagcagcggg gataacataa aacaacatag ataaatcaaa
    27721 gcgcataaag ctacttgtta aagcaatcgt taccaatttt gccagttgag aaaagaaaat
    27781 cgtagataga gaatatagtg ttgcttcttt tattggcaat gcaaacatca gcatcaacaa
    27841 agaaacattg actggtccac caccaatacc taaaaaacta gctaaaaagc ccagaacccc
    27901 ccccacatat aaggtaccaa gtccatgacg tcaaatgaaa ttttggctga tgatgcttgg
    27961 tgtagaagaa cgcaaaaacc aaggtcaata ccgtcaaaaa aatttggatc agctgcacat
    28021 gcttctcgtt ttcaaataac tgtaaaaaaa cttcgaatac aatgttgccg actactcctc
    28081 caacaacagc tccactagat acccaaccga caatctgcca attcagtgat ttcccgcttg
    28141 atacttgtcg cacagtcgaa acaatggaca tcgtgaatac cgcaactgtt gagtaaaaag
    28201 aaatccctgc aacagaatgg gcgccgataa gatcaagaat cggtttgatc aacacgcctc
    28261 cacccatccc ggaaatagcg ccaactgtgt tcgccagaac gatcaccaca aaataaatca
    28321 gaccaatcat cttgtatttc ctttctcttg tttatatctt ttagcgattg ttactctttt
    28381 cttttattat acagtaaaag aaacaaacga ggcttcgaaa gcttttctac tttttaagga
    28441 aatttagtgc taaacgaaaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
    28501 agccaaacaa aaatagctcc tcataatttt cgtttggttg aacttattac cgcagatgca
    28561 agcttttgaa caccgtttaa gaaagaggtc gtgagcctgc cgttcagctc cgagtcacaa
    28621 ggaacaattt caaaaaacag cttctcatgt ttgttgaata ttgtgacttg tgaccgagga
    28681 gttggcagga gaacaccgtt taaggaagag actgtgaagt caacgttttg acctgattat
    28741 tagagaaaca aatggcaaaa tagttcttca tatttcgccg tttttcgagc gaatgccgag
    28801 gggcagctga ttgaacatcg tttatttgga aaaagggatt gtgacaagac gtttgtcaca
    28861 atcccttttt ttatatctta ttaattcatt cttttgttct attcttcttc ttccgccatg
    28921 aatgagaaac ccatatcaaa gaactataaa ggttatcaaa acagcgttta tgactaaatg
    28981 aattagagtt atctatcact atttttcaag cgatttaaac caaatggcat acaatatggc
    29041 atacaaaaac ttcaataagc cgtgggggtt tttaaatttc ttcttgttct ttaaaatcaa
    29101 tgagtggagc tacataattt gaatccactt tttcaaacgt cactttttgc atcgacgaat
    29161 gttttctttc ccaagataac gtaaatagat ttgactctgg acttataaag tctacttcac
    29221 cttctagtat tactttctta tgcaaatatt cctctaataa atatagactt gctaaagcat
    29281 acaacacatt ttccaaattt gcttccttat aatttgaaga acgaccatgt tttactctgt
    29341 tatatatttt ccaccaatct ggtgtatctt caggccaccc atctccaaaa ggttttagtg
    29401 aagtcaatgt taaagaattc ataaccctaa caccattatc caaacacatt aattcgctag
    29461 atttaataac agctttaaaa tcagttatgc ttttattgct actgctatca aatccgcaaa
    29521 gttctttgaa aataatctca cactccgaac caatcgctag tagtagtttt tgatattcaa
    29581 tagagaatgt tttgaaatta ttctcatcga gcatcacaaa atctgtggta ttaataaact
    29641 cattttctaa atgtagataa tacttccaat aaaaatttgt aaactctgct ttgtccatta
    29701 aatcacctct tttgaattat aacaaaaaaa acacgtccac aacagacgtg ttctttgatt
    29761 tctacacaat cagcttgcca aaaccatcca atcagtccta gcatgcctgt attaaaattg
    29821 aacgttcatc atctatcatc tttcttagtc cagcatgttt gcaaccacac ctacaatttt
    29881 cagtccaaca atagatttcg actagctaag tcttgcaaaa ttcaggcagc taacccttca
    29941 tttactacct taattatact aaatacccac aacgtttaca agtgtaatca ttctaagaaa
    30001 tctgtttagc tatgatcgtt gtataatctt taaacgccgt tccttcattg attgaaataa
    30061 ttttataact cttgtttttc caagctactt tcatatcgtt tgttagttca taatcttgat
    30121 cgtatcgaat aataaacgtc aacgttcctt caagaatggt acctacacta gcaagcacgt
    30181 cttttagcat ttgtgaacgc acattagccc aacattcaaa tacagtttct tcactatcca
    30241 ccatctgccc atcttcatct tttacccgtt ttgtcttttt aaacttgact ctctcagtca
    30301 attcatttac attcttaatg attggcataa taagcacctc tcatttgttg tactaatgct
    30361 tgtaccgtta ctggtatccg ttcgctgctg gcttcttgac ggttcaaata ccaatgttga
    30421 gtaagtaatg acaccgccaa atcaaattgc tgataattat ccatatcttt atccgttgcg
    30481 ctactatcaa tagcactttt tatatattcg cctgctgtat caatcaattg ttgaataaag
    30541 tcatcatcaa tagtgtgatc gatccgcata ctttttttta tgctatccaa aattaccatt
    30601 ttatctctcc taatgaaaaa aaggcagtgg gataaaccgc cgcccaatat ttttctatac
    30661 ttttggtgtt acaactggcg taaactcaat atacactgaa gcattttgat caattttctt
    30721 ataatcgttg cgaacgatca ctgcaagtcc ttgtgagtag taatcgaatt tttcccattg
    30781 agtcgttact tgatttctac gagcaacaaa gatagattgt gcaatatccc ccataatcat
    30841 agggaatgtt ccagctttag gattggcaaa caatgtatct gcaatcaata ctactggcat
    30901 accgaataat gatttaccac taggtgctgt cacatcgggt tgtaaaatgt aacgtccatc
    30961 tgaatctttc aatgtatcta ggtggtaata tccgctttgg tttagaatta ccattttatt
    31021 taatgctggg tctaatgcca cattgtatag ttgtttcaaa tcgtccaacg tagcagccgt
    31081 tttcttagtg aatgttttta ataaatccat gatatgctta ttgtcagtgt tttctaccaa
    31141 tttcgctaac tgatctttga cctcttgtac aatattcact gctgaatctt ccacaacctc
    31201 atttgataag gcaatcttac cagcacgagt ttctacttta taatcaactg aagtaaacat
    31261 ttcagcgtca atatcaccaa tttcagcaag ttcagctttt gttgctaaca ctgcttgttg
    31321 gttagttgct actggatatt taccttgtcc atttgatact gtttttactg ttgcatattg
    31381 agctaagtta taatttgaac gtttcaaatc aaagacttca ccgattactt cttcgggaac
    31441 aactgccgct gcatttacag tagtgactcc atcacgcact tcgccttgtg aacggatata
    31501 ttcttcatac ccacgcaatt ctgttttttc gttatcgatt aaagtttttt ccatgttatt
    31561 actctccttt tgtgctttag aagtggattc taagctattg tttgtttgtt ttgcttggtt
    31621 gttactcata aacgattcgt aagaacgctt atttacttgg acatttgatg aatcataagc
    31681 gggaacggtc acgacactaa tttcattaag tgctttgatt ttatctattg atcgttcaat
    31741 tgtgccatct tcttttttgt cgaatgaatc taatccaagc acgaacccaa aggacataga
    31801 atcaacaacc ccttttgaga tattctcata cacatcattt gaataggtcg tgtcggttag
    31861 ctctgcttca aaatgtaatc ctgtatcatc gacattcaat ttcaacgtac ctgctttgac
    31921 actagctagt ggctttgaat aatcgtgtcc atataataag aacacatttg ataaatccac
    31981 ctcttttaat gcttcgggtg taatcacttc tacaaagccg cctaaatctt ttgacggttg
    32041 cccgaatttc aacgcatagc ctgaaatagt tttgccgtct gttggttgtt tttcattttc
    32101 tttctctact ttgggagaat cggctgttag ctcagcttct tctgtcagcc gtttttcctt
    32161 ttcttcttca ttattcaact ggtgtaactc cttcctgttc ttttaattga tatttctcca
    32221 acgtatctaa atacgtaaag ttcaaacttg ctagtaaacg atcgccacca tccatggggg
    32281 gtagccccat tttcgaccgc ccctcattaa tcgtgagaag tgaccctttg acctgatcca
    32341 atacattttt tacttttgtt tcaggatcag tctctagtaa ctgatcggaa ttaaagcgga
    32401 tattggtttc aagtttcgta tctaattcac tcacaaacac attaaagtaa tgaatcagcg
    32461 tactttggat atactggaga ttactttgaa cggtgctaga atgttcattt tcaacgccta
    32521 aacgctcaat cggtacacca aacgccttgg caatttgttt tgtgttccaa tcattggaat
    32581 tgataagctt caaaacatcg gtgtttattt ctaatgtttt gtagtccatt gtttcatcaa
    32641 gaatgatcgt tctaagagca ttatctccac tacttgaacc gttggcttct tcaaactttt
    32701 cacgaattgc gttctttgct gatccgtcta aatctgactt atgaaccttc aaaatacctg
    32761 acccactgac accacgagta aagaaattgt gcaatgtgcg gttgccagct tgttgaattt
    32821 tcagttcatc acgtaacgca taaagtggtg gtaatcctgt taaaccatct tgggtgaaat
    32881 atttgaagtg caaaatatcg ctagacttca cacgtctttt tttatcacca atctgataag
    32941 acaacgtacc attatctaat tgagtaaccg ttacacttga gttaggtaat aagtgaattt
    33001 cttctacctt ttcaccgttg cgtttaatct ctgcaaagct attaccgttt agcaacatat
    33061 tgaccgccaa agcaaacttt aaatgccaac catccatttc tgaattaggt ttctcgttta
    33121 gtaacttcac cagttcatta tcagcttgtg gcatattgtt tttgaccaat tgaatcggac
    33181 ttgaagcaat atcactagca ataattcgaa cggctgcaaa cacatcacta tttcttaatg
    33241 cacgaacgct tgtaaagctg gtggtgtaat catccgattg gattgatacc acatgttgta
    33301 ggaaaggatc tcccgttgta ttttgtctcg ctttaaaaaa tgccatcttt taacctccct
    33361 tctgttctgt atttagcatg accgctaccc caataagcac tatgcccagc gctaacaagc
    33421 catagtagac atttgttagt aaggtgatag caaccaacac acacaccaat ccaagaagta
    33481 aaagaacggt atgaatgtaa ttagaaagaa aattcttcac ttgtataaaa ttcattgtct
    33541 gctttcgctc cttccgttgt ggtgaagtaa tccattgcaa acacataggc attgattaat
    33601 gcagctattg gatcaatttt gttactgttc tttgatttat taatctgaat gccgttgtta
    33661 tccgttttta aaatagcgtt gttcactgca tgggtgagaa tcgtattttt attgtgaata
    33721 acgttgcctt cataaacttg ttctctaaaa gttcgggtcg gaacgtttag agtaatcgtt
    33781 ccttgtctta cttctaacat tgggtagttg gctttttcag ctttactgat aagcgaatta
    33841 gcgttatacg ggtcataaca aatccccata cattccaaat cattttcttg aatcaaatct
    33901 tgaataaact gaaatacttg gtcatagtcc acgattccac tttctaattg agtaatggaa
    33961 cactcaccag ctttttctag ttctcgataa ggcaaaccgt cgcgcttttc tttgtcttga
    34021 agcccatatt tggtggctac aaagctatgt gaatcacaat aaagttcgcc gttatctagc
    34081 ggtacgatcc atgaaacgct agttaaatca tctgttttag ataaatccac tccgatataa
    34141 acgggcttgc ctgtaatatc ttgtggttct acttcggttg cctgccagtc ttcagcaatc
    34201 atatagctgt cctcactcgc ttgtctccat aaattgaagt ttttcaccaa tacagaatta
    34261 aggttatttt gtttcaaagc aaggttcaca tcatcttgaa tggttggaat cattactttt
    34321 ttgatttctt cactttcaaa aatcggattg gctttgatcc atttctcttg atcgtgaatt
    34381 tcttcgggat cgtccaattc ccatatcgct ataaagtacc tgtcggcttg ttcttcgcct
    34441 tttagcacac gatctaacaa taagtattcc tcgtacatag gaacgtttaa atcaagccca
    34501 gcggtactaa taacacacaa taagccgttc ttttgttggg tcattcctga cttaatgacg
    34561 ttgtacgttt tacgagtttt tgcttcgtgc cactcgtcaa gaattgcggt tgttggtgca
    34621 aacccatcta gcgtgctggt ttcactagcc agtgccatag cgaacgaatt agagggcaag
    34681 tgctgaatct tagagttcat aattttcagt tgtggtctta aaaacttact agacttgacc
    34741 acgtttctaa gtgaattaga cagcatatca tagcccaatt tagcttgttt caaagcgtta
    34801 cttacaaaca atacttgcct tgcttctgct ggttcttgtt ccataatcaa cgcattggca
    34861 gccatgccac ttgcgatata cgttttccca ttcttacggc tcatactggt aaacgctcga
    34921 ttgaatcgcc tgtaattccc ttctttagta cgccagccat aaagactgcc tagaatgaat
    34981 ttttgaaatc ctaacatctt gattgcttta ccgtccgtag atggtagcaa ctcaatgaat
    35041 ttaatcgctt tggtggcttg ttcttcatcg aacacataag ggaagctatc tgacttagaa
    35101 cgctccaaat cgcttatatg acgctcacac gcttggataa ttttcttggg tgcaatcagc
    35161 ttacctgata ggactttctc aatgtaatca ttcatgaatt catcaactct ttgaacgggt
    35221 cttcgggttc atcattttta accttgttca aagctagttt tgatcgtgat tctaaggtca
    35281 tacctagtga ggtggcttgt tttttcaaat cactcatagc ttgtgattga acccgaaccg
    35341 ctggattggc tttctttact ccgttttcgc atgtgataaa cgtacctgat tttcttatct
    35401 cggcttcggc tgttttcatt cgagcaaagg ctaaacaata ggctaccaat gaaccataat
    35461 ctgcttcact caatggataa tcttttttta atgttggtac gatacgattc cattcacttc
    35521 tagcgctcgc tgccatccag tcagggggcg tgatatttat taatggttgc tgcttgaaca
    35581 attcttctct agcgtccaca cggattgctt tttcttcact tgatatattg ccttttgtat
    35641 cgtgtaatat tttagggtgt cccatgttgt cctcctttca gacaaaagaa aaaggaacgt
    35701 aagcaattaa gctcacattc cttcgttttt cgatcagaat ttttctttat ctaatttatc
    35761 tatataaatg atttttccgt tctgcatttg aatggtcact tgaccaaaat gaggtaactc
    35821 aatggtgcgc tgtttccctg ttttatcagt aataacaatt gtatttttca ataaatctcc
    35881 tactttcatt atatactttt tcacttgtga ttacaagtgt aatcgttatt tgtaattacc
    35941 cttatgcctt ttcacaacgg ttatttgtac caaaatgagt aggcatcgat tgtcagtaag
    36001 tttcacacta tgccccctta actcaaaggg tactcttttc tcgtctttgt ctttcattgg
    36061 ctgtcttgga attatggtgt gcatggcata aagaacgaaa attagtttca tctaaccgtc
    36121 tattccaatc atcttttagt tcaattttgt gatctacaac atcagctttc cttatcaagc
    36181 cttcttctag gcaatcctca catactggat gatttaaccg atacaagtat gaagcctttc
    36241 gccatgttct actttgatag aattgaaaat atttaccttc gctttgcttt ctttcttcac
    36301 gcttaattgc cgtttgcttt gctttatgct tcccacaata cttctgccta taatcaatca
    36361 atgttttaca tccagcatga ttacattgtt ttttaactgc catagattac caaccaaagc
    36421 gatctcgttc gtcagaatca tcttgttccc attttgattt gtattcaata taaggcgaaa
    36481 ctatcttcaa gcacgttccg tctttaaaat taactttagt gattttatct tcctcaaaac
    36541 taataaagtc tacatcttct tgagtgtttg aatactcaat gtgtttctca ccatcgaagt
    36601 atctaatact ttccatatgc ataattattc tacctccaat aaattagtta tcaattcatc
    36661 atctgttagt agcaaagcca agaacttatc atggatgttt aaaaacaaat tatcctcatt
    36721 cgatgtaagc tctcgttgtc tcgctcgatc atataggtta agcagacaat taaattcatc
    36781 cattagctca cctgtatcat catagttgcc actatctacc aatgatccca ctactagcga
    36841 ttgacaccac gctactgact cctgcagcca atcaggttct tcttccatta tctttaatac
    36901 ttctggatca aatgtggaat aatcttctgt taccaatccc acactaaatg ccagcgtgtt
    36961 cattagaaac cgatccattt catctgtata ttgaaagttc ggtgctttgt ttgttgaaat
    37021 aatattgagc atttactttc ctccttgatt cgtaacacgt ggtaacacct acgtaacagg
    37081 tacccgttac cactcaatcc caacggggac aagggttcta gcgttttggt aacaggtaac
    37141 gggtaaaatc cagtaacatt attccccagc atattagcta actatttcaa aagttttaag
    37201 gaaataaggt gttaccccgt taccaaattg ctgtatccct tggggcagta agggtaaact
    37261 agtaacgggc taggtgttac tgacccgtta ctacccgtta ctttttgtac tttccagctc
    37321 gatttttatt ttcataggca tttgacttat tgtaaaatcc tcgtcgttta tttctttttg
    37381 gcagttgctt ccgttcccaa ccaggaatac tgctaataat caaggcaatc tttttagttt
    37441 ctgcatctga tcgaccattc aataaatcct ttgcttgttt atcaaaaaca gcttccaaaa
    37501 tatcagcggt gagtacgcta tcaactaaat aaaactcttg ttgcatagta gctccattat
    37561 agaaatgctg taaatttttc tcattacatt tactgtcagg atagttctga taataagaac
    37621 gtctcgccca cgctggagct tcttcccatt tatacgggat ttccatttcc aaatatttag
    37681 tgattgcttc tttaattgga ttctccatct tggcatcctc ttgatagtct tttgcaattt
    37741 ctaagacttc tttatcttca tcgggactaa aatagattcg ttgtccttta tcaaaaagca
    37801 cctttgcttc agctaacact tgaagaaaat attcatcctt tgttttaaac ggattcatct
    37861 ttggcttatt cttacaaggc aaaggataaa aacggcggtt tcctgtatca tctttcaaat
    37921 actgcccatc attcgacgtt ccaataaata cacattgcct tgcccaagct atgacattcc
    37981 gaccataagg aggtctaatt ttatcgatct tggcactgat gaagtttttt atctgttcaa
    38041 ttttagtttt gttcattgaa gataattctc caagttcaat aattacattg ccttgaagct
    38101 gcatataatc atctttcttt tcacctagcg aatctaatga atcggtgaaa aaatcatccg
    38161 tgtaaagtaa tgctggaacg gtactcttac caatcccttg cggtccatcc aaaactggaa
    38221 cgatctcaaa ctttattcct ggtttataga tacgtgcgac tgatccgact atccaacgtt
    38281 ttgttacttc tcgtgtgtaa aggttgtcgt ctacccctaa aaggtcaata aacagtgttt
    38341 ccagtctagg ctgttgatcc catttaactt tttcgatacg ttcttttact gggttgaaaa
    38401 cattctttct agccacattt actactgctt tgtgtatttt gttgtcatca aaaactacat
    38461 tgtagttttc ttcaatatat gccgttaagg catcctcaaa actgtcttcc cattctccag
    38521 cggtacgatt gatccaagga gaatctttta ataggtgaat gtatccgcta aattcgttat
    38581 aagcaattgt tccagctaaa ttgctatcat tggttaaaat caattctgca ttcacaacac
    38641 tgttactttt aatctgatcg tctttattgc atcttaatcg tgacatccag gttttcaatt
    38701 caatgatatt gtcttcacgc tcgctggctt cgggaaagtc taaaatacta tctaatttat
    38761 gcttatgagc catgagcttt ccccctcaaa tcttttttaa tcatcgatcg aataatttga
    38821 ttcacttctt tatcttttag cggtggttca ttgtacgcat ttgctgtata aatcaattct
    38881 gctaaagttt gattatctac accgtgccat agtaaccaac cgcataactt agttaaatga
    38941 tcgtttctac taccttcgcc agcgccttga acaatcatat ctaatttttt tgctgtccct
    39001 gtttttgcta cgggtcggta ttgagtcttt atttcttgcg gtttttttag cagtaggttc
    39061 actagccaat cgggagctgg tgcaatatta tttagagata cgttttcaat aggttcatat
    39121 gctctcccaa cacttcttga aataatcata gttggcgcta ttggaactcc tttagtgata
    39181 atatcaatcc cgcttaaact tttaccttta tcatcacgcc cgaagatatc agcaaatgca
    39241 cctgtttctt gttggataat tacaccatca ggaattttaa agaagaaatg gattccttta
    39301 cctggtgtga tttccgtata agtagaagga aatggatcat acattgaagc aagtttttta
    39361 taattctcaa cgccgtttcg tgtttcagaa tgttgatcta ggtcaactac caccatttga
    39421 tgatcttcaa gcattaaccc aatatttcta gttgcatctt ccgtccacca ttcattaatt
    39481 tgattagaat cgttagttgc atcattccaa ccactagagc cttttattgg aattttagtt
    39541 ccaggagata agggataaat tgaccatccc gacttttgta attccgttgc tgcttgataa
    39601 ttatttgcca ataaacctct cctccttttt caaaatactg gtataaatca ttcagacacc
    39661 tccatgagct tcaactgctc aagttcgtat ttcaacccat cccgaacaaa ttcagcacct
    39721 tttaattgat tctgatactt tacccatgta ttaaatgttt tcacccttgc ttcttgcata
    39781 tcagtaatgt ttaaccatgc ggttaaaatc tgatttttta gctcgttgat ttctcgacaa
    39841 attattttgt gttctgcatg ttttttgagt acatacaaaa ctgttagttt ttcattgaat
    39901 gaatttccaa agtaggtttt cccgatatta ttttctagtt cagcgatccc ttgattaatt
    39961 tcctctaatt caaggctatc gagttggttt aatagttttg ttttgtacat ctacttatgc
    40021 cctccagttc ttaattgata gtcgtactcg tcataagcag tgaagagata taacgctaat
    40081 cctgcaaaga tagctatttt tgcaattaat ggtaataagc cgaaaattcc gccgattaaa
    40141 atgcttccta taattaagtt tcgtttcata atggttccct ccgatttttt aatatggtat
    40201 aatcggaaca aagaatatcg ggttttatcc caattaagtt gcctttatcc tgtccgccaa
    40261 gactgttatg gataaggctt tttctttgtt ctcgatcatg ttaatgctcc taagttgtct
    40321 gcaattcagc agaacgatta taaattttct cactcactga aaactgcaac ccatattttt
    40381 ctttaacagt cattaattct actgtctcat ctaaaatagg ttcacgatca accaacattt
    40441 caggagtcat atctttcttt tttaccattt tggaatgacc ataaatagtt gatacagctt
    40501 tattggctat cacattcgct ttaatgtagt ctactggttt tggttcagta atagcgtggc
    40561 ttaatttagt cattgcttct ttttggtgtt ctttgtctag catgcggaat acttgaaagc
    40621 cttcaagtcc tgtggactgg cgtagttctt tgatgatgtc gaaaatccag tcttgaaatg
    40681 cctcggcttc ttttttacgg cttttgaatg ccatgcggta aatatttttt ttcgttgatg
    40741 atgtttacat cttgtgttct gcccaatgaa tcgatgacct tactagtagt aaccccatct
    40801 ttaggcaaag agtgaatggc tctagtaacc tgtttaagac ctaacgcttc cgcaacatct
    40861 ttcgccactg cccaccattc atcattaata tcgacaaatc ttataatgtg tccgttccaa
    40921 atttcagttt tcattcaacg tcctccagtt caagccctag caagctcgct aacaactcaa
    40981 cttgctgata caaaatttct tgatactctt caatagaaag tcgttcttta aactctgctt
    41041 gtactaaatc gttcaagcca cgcaatactc cgcttaccgt tggttcttgt ggtagcaatt
    41101 tttgatagtc catttgttcg tacaaacatt caaacgtttc ttctagcgct ttgccttcca
    41161 agtttgctaa ttctgccgct gtgtcttctg taagaattgt tgataatact ttttcgattg
    41221 ctttattttg tgtcgattcc attattattt cctcctatta atccctttgc ccccacggct
    41281 tgcgtttatt tttggtattc ttccataaag cgatcacatt catcttgtga aattcttacg
    41341 actccatcaa ctataatcaa ctttaaaccg ttaggaacat atttctgcat catagtattg
    41401 tatgaaacgt tcatatactt agcagcttgt gccttgttca tatattttgg aaactggtac
    41461 tttttttcta ataatgaaag taacgttcct tgagtttgct ctacttgttg aacaactcga
    41521 tcgtcaataa ttttaaaagc tggatcatca agcgtgagtt tgtcaatttt taattccata
    41581 tttatccccc cctcgttagc atacatttct atacaaagat tttaaaaaaa tgcaagaggc
    41641 tcttgaatac atttctatac ctcttataaa ctcaatatac catcatacct tttcatttgc
    41701 aagaatttta acatactttt ttgaactttt atgtttcaca catatttaaa tcgtgatata
    41761 gtagagaaaa tatatgaatg gtggtgaaaa tgtggctaga aaaaaatacc ccgaagcagt
    41821 ggagacagga caaagaatta gactggctag aattgaaaag ggactctcaa tggaacaact
    41881 tggaggtaaa ttatccccac cggccagtaa aggtgccgtt agtaattggg aaaatggcta
    41941 taatcttccc aataacacca ggcttaaaca actttcaaag gtattagatg tatccactac
    42001 ctatctatta aacggatctt atacattaag agatatgcat atgatgcccg aaattaagcg
    42061 aaatgtgctt gaaaagcaat tgaaaaaaac tattgaaaaa aataactcta taattgattc
    42121 taatctttct aatgctatca ataacatcga agaagataac ctgactgtct cacagaagat
    42181 ttatttaaca aacgcaataa aatatcttaa aacaggatct cgaattgaat ttcctgacgg
    42241 gagtacccca attgcttatc tcgcagctct tctaaatacg cttttacgat atgactcatt
    42301 aattaatgat tcagaaaaaa atacagatga gaaaaaagag tatcaagaat tttatgaaaa
    42361 tgatcttcta aaagagttca cagctcttgt tactgagcta aataaacact attcaaaata
    42421 ataataaact gcctcctcca accgccccca cggcttgcta cactatggag ggaaaactat
    42481 tatggcaaca ttcaagcaat atgaaactaa aaaaggtaaa ttttggctct atgaagccta
    42541 tcttggaatg aacaaaatga ctggcaaacc tgataaggtt cgtagacgag gttacaaaac
    42601 aaaaaaagaa gcccagcttg ccttgtcacg cttgcaagtg gactacgata aaaacggtat
    42661 taaaaaagcg aacaacgaaa cgtttaaaga agtttatgac ttgtggctag aaacatataa
    42721 aaccactgtc cgagaagcca ccttcatgaa aactgaaatc aagtttagaa agtggattct
    42781 tccaaaatat ggaaatcttc gtgtgaatga agtcacggta aaaaaagccc aacaaattgt
    42841 aaatagctgg gctgaaacta ctgaccaata caaggtactt cattcaactt ctaagcgtat
    42901 ttttagctac gctatcaatc ttgggattat tgagactaat ccactagaca acgtattaat
    42961 gcctaaaaga accaccacta agcataaaga aaaggtcaaa gtttacagta aagaacaact
    43021 gatcaccttg tttgattacc taaacagcaa acctgaaact tatcgaaacg actttgataa
    43081 aacgttacta cgctttctct tttatagcgg ggttaggatc agtgaagccc ttgcattgaa
    43141 ttggtcagac attgattttc aagaaaatac ggtcaccatc aataaaacac taagccaatc
    43201 caaaaatggc tataaaatca gtgatcctaa aactgattgt agtgctgggg ttcttccact
    43261 agatgaaaga acaatttcta tattaaaaaa atggcagatt aatcagcgga aatatatgct
    43321 gacacttggt gtaactgatc cgactatgat tttttgtggt atctataaag aactaatcac
    43381 acaccacgct atttatgcaa gattgattac tatttgtgaa aaagctatgg ttccatttct
    43441 cggtgtgcat gtagcaagac acactcatgc ttcaatgctc ttggacgctg gagctactat
    43501 ggtagaagtt caaactagat tacggcatac aaaaattagt cagacgatcg attgttacgg
    43561 tcacttggca aaagaaacga aagaaaagac tgttgaaaaa ttagtacaac acttaaatat
    43621 tgactagatg gcatacaata tggcatacag taaaaaataa aaaaaggaga aaccctatca
    43681 tatcaaggtt tctcctagct ttatagtcta ttcttcttct tccgccatga atgtattgaa
    43741 tacttcttca atcatgtccc attctgcgtc tgtttcgatt tgttctaact cgccttctgt
    43801 tccatcttcg ttttcgatgt atgcataagc ttgtaattct acatcttcat cttctgaaac
    43861 accagcagga tataaaagaa catagttacg tccaaattct tcttgtccgt caactgttag
    43921 taggatttca tataatgttt catttccttg gtcatccacc aatgtgatgt gttcgtgtcc
    43981 ttcatggtcg tgggaatggt tatgttctgt cataagatta cctcttttct tttattatta
    44041 aaataattgc ttacctgagc cgtctaaata attttgtaag atcatcacag ctgctaattt
    44101 atcgatgact ttttttcgtt tggctcttga agtatttgcc tgctctacta gcatacgttc
    44161 tgcctgaaca gtggttaatc gttcatcttg ataatcaact ggcaaggaga atagttcttt
    44221 gatcttttct ccgtatgaca tggaagcttc tgcccttggt ccgatggaat tgttcatatt
    44281 tttaggtaat ccaactacaa accggtctac ttcatattgt gctactaatt ccttcaaccg
    44341 atcaaagcca aactcttctt ctgcttcatt gatccgaatg atctctactc cttgagctgt
    44401 ccagccaaag gggtcactga tagcaacacc tactgtctta gaaccgacat ccaagcccat
    44461 tactctcata aattcactcc atgatttgag aggtagtcct tgacaattac ttccatgatc
    44521 tcatcgcgtt cgtgacggcg aatcaaattt cgagcatcac gataacgagg aatgtaggcc
    44581 gggtctccag aaagcaagta gccgacaatc tgattgattg gattgtagcc tttttcttcc
    44641 aaagcccgat aaacaatcgc taatgtttcg ctcacttctt ttttgcgact atcgtcaaaa
    44701 ccaaaacgta cggtttcatc tgtaaaaccc atgattctac acctctttcc cgcaaggtaa
    44761 ttccagctta ccttctacaa ccttatttta ctcgaagaaa gggaaaacta caaacaaata
    44821 ttccaaactt ccgaataaat taagaaacac tatttgtatg tttttttcct tttttctgta
    44881 gctttatcaa aagtactc
//
LOCUS       DAPCMT010000009        43540 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPCMT010000009 DAPCMT010000000
VERSION     DAPCMT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 43540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.009"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(179..2560)
                     /locus_tag="RD743_000387"
     CDS             complement(179..2560)
                     /locus_tag="RD743_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDU0562246.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2550..3197)
                     /gene="recU"
                     /locus_tag="RD743_000388"
     CDS             complement(2550..3197)
                     /gene="recU"
                     /locus_tag="RD743_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDU0562247.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3261..3800
                     /locus_tag="RD743_000389"
     CDS             3261..3800
                     /locus_tag="RD743_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDU0562248.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3890..4309
                     /gene="gpsB"
                     /locus_tag="RD743_000390"
     CDS             3890..4309
                     /gene="gpsB"
                     /locus_tag="RD743_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDU0562249.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4331..4698
                     /gene="rnpB"
                     /locus_tag="RD743_000391"
     ncRNA           4331..4698
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RD743_000391"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4907..6073
                     /locus_tag="RD743_000392"
     CDS             4907..6073
                     /locus_tag="RD743_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDU0562250.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6119..7615
                     /locus_tag="RD743_000393"
     CDS             6119..7615
                     /locus_tag="RD743_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDU0562251.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7696..8379
                     /locus_tag="RD743_000394"
     CDS             7696..8379
                     /locus_tag="RD743_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDU0562252.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9024..10214
                     /locus_tag="RD743_000395"
     CDS             9024..10214
                     /locus_tag="RD743_000395"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDU0562253.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10316..11269
                     /locus_tag="RD743_000396"
     CDS             10316..11269
                     /locus_tag="RD743_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDU0562254.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11338..12687
                     /locus_tag="RD743_000397"
     CDS             11338..12687
                     /locus_tag="RD743_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0562255.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12870..13193)
                     /locus_tag="RD743_000398"
     CDS             complement(12870..13193)
                     /locus_tag="RD743_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562256.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13322..14485
                     /locus_tag="RD743_000399"
     CDS             13322..14485
                     /locus_tag="RD743_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDU0562257.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14555..15904
                     /locus_tag="RD743_000400"
     CDS             14555..15904
                     /locus_tag="RD743_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDU0562258.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15949..16380)
                     /locus_tag="RD743_000401"
     CDS             complement(15949..16380)
                     /locus_tag="RD743_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0562259.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16768..19410
                     /gene="alaS"
                     /locus_tag="RD743_000402"
     CDS             16768..19410
                     /gene="alaS"
                     /locus_tag="RD743_000402"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDU0562260.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19610..20206
                     /locus_tag="RD743_000403"
     CDS             19610..20206
                     /locus_tag="RD743_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDU0562261.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20328..21476
                     /locus_tag="RD743_000404"
     CDS             20328..21476
                     /locus_tag="RD743_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDU0562262.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21514..21927)
                     /locus_tag="RD743_000405"
     CDS             complement(21514..21927)
                     /locus_tag="RD743_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDU0562263.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22006..22437
                     /locus_tag="RD743_000406"
     CDS             22006..22437
                     /locus_tag="RD743_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDU0562264.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22536..22769)
                     /locus_tag="RD743_000407"
     CDS             complement(22536..22769)
                     /locus_tag="RD743_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDU0562265.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22952..24622
                     /locus_tag="RD743_000408"
     CDS             22952..24622
                     /locus_tag="RD743_000408"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDU0562266.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24769..25155
                     /locus_tag="RD743_000409"
     CDS             24769..25155
                     /locus_tag="RD743_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562267.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25142..25840
                     /locus_tag="RD743_000410"
     CDS             25142..25840
                     /locus_tag="RD743_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDU0562268.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25845..26309
                     /locus_tag="RD743_000411"
     CDS             25845..26309
                     /locus_tag="RD743_000411"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDU0562269.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26306..27217
                     /locus_tag="RD743_000412"
     CDS             26306..27217
                     /locus_tag="RD743_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDU0562270.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27452..27991
                     /gene="pyrR"
                     /locus_tag="RD743_000413"
     CDS             27452..27991
                     /gene="pyrR"
                     /locus_tag="RD743_000413"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDU0562271.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28005..29288
                     /locus_tag="RD743_000414"
     CDS             28005..29288
                     /locus_tag="RD743_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDU0562272.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29301..30227
                     /locus_tag="RD743_000415"
     CDS             29301..30227
                     /locus_tag="RD743_000415"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDU0562273.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30246..31526
                     /locus_tag="RD743_000416"
     CDS             30246..31526
                     /locus_tag="RD743_000416"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDU0562274.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31531..32607
                     /gene="carA"
                     /locus_tag="RD743_000417"
     CDS             31531..32607
                     /gene="carA"
                     /locus_tag="RD743_000417"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDU0562275.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32612..35797
                     /gene="carB"
                     /locus_tag="RD743_000418"
     CDS             32612..35797
                     /gene="carB"
                     /locus_tag="RD743_000418"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDU0562276.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35851..36627
                     /locus_tag="RD743_000419"
     CDS             35851..36627
                     /locus_tag="RD743_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDU0562277.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36624..37556
                     /locus_tag="RD743_000420"
     CDS             36624..37556
                     /locus_tag="RD743_000420"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDU0562278.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37553..38269
                     /gene="pyrF"
                     /locus_tag="RD743_000421"
     CDS             37553..38269
                     /gene="pyrF"
                     /locus_tag="RD743_000421"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDU0562279.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38276..38965
                     /locus_tag="RD743_000422"
     CDS             38276..38965
                     /locus_tag="RD743_000422"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDU0562280.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            38938..39207
                     /locus_tag="RD743_000423"
     CDS             38938..39207
                     /locus_tag="RD743_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562281.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39245..39886
                     /locus_tag="RD743_000424"
     CDS             39245..39886
                     /locus_tag="RD743_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDU0562282.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39929..40621
                     /locus_tag="RD743_000425"
     CDS             39929..40621
                     /locus_tag="RD743_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDU0562283.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40618..41520
                     /locus_tag="RD743_000426"
     CDS             40618..41520
                     /locus_tag="RD743_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0562284.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41678..43384)
                     /gene="efbA"
                     /locus_tag="RD743_000427"
     CDS             complement(41678..43384)
                     /gene="efbA"
                     /locus_tag="RD743_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDU0562285.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13937 a         7683 c         9468 g        12452 t
ORIGIN      
        1 atgataaaag aaaaatggat tttctaattt ttaagctaaa tgttagatca cacatgtata
       61 tgctggatag tcttataaaa aatcatgtaa tacagcttgg tacatgattt ttatttgtag
      121 ctgtttttta catagacaca aaaaagaaac cacgcttgta gacgtggttt cttttttact
      181 atctgtttcc agaagctgag gtgctgctgg atggtgtatg gtcatttgca gcatccgctc
      241 cgctagttgc tggctcactg gaagaagcag gaggagcaga tgaagacggt gtcgagcttt
      301 cttcggatga agaagaagga accgttgttg aagacgctgg ctcgcttgtt tctgctgtcg
      361 tgctttcttt tgaggactca gcagttgatt cgctttgact agtagagcta gagctttgag
      421 tcgttgtctc tgttgttgag cttccaggag tctggacata gctggaagat ggaatagttg
      481 ttgatggtgt aattgcattc gatctagctg tatattgatc tttgtagtac agttcaccgc
      541 ccacacgaat taatccgctt ggcatctccc agtctgtatt tgtcacgtta gctgaaacat
      601 actgcataag ctcgcgataa acatcagagg ctacgtgtga cgattctgaa gtgacaggtg
      661 tcattttttt attgtaacct gtccacacgg aaatcgaata atttggcgta tagcccgcaa
      721 ataggatatc tggatacacc ccagaagaaa taccaagttt tgcatactcg tcatctgtgt
      781 aattggatgt tccggtctta ccggcttgat ataacccagc gatttgcgca ttcgtaccag
      841 ttccttcagt gatcgtatct tttaaaatgt ctgtgatcat ataagctgtt tctggagaca
      901 tggctgtttt tccatctggc tcataagttt cttctgttcc gtcttggaag acgattttat
      961 taacatattg aggtttgtaa taagtccctc cattggcgaa agccgcatag gctgctgcca
     1021 tttttaagga agaagctccg tattttgtcc catcttgttc ttccgtatta ctagagatcg
     1081 cattcgattg atggatcgta ctgtattcga tcccaagatt ttttaagaaa gaagccactt
     1141 tatctgaacc gacttcattg aataatttga ctgctggaac attacgggat aagtaaagtg
     1201 cttggcgtaa agtaatggtg cccatatatt gattatccca gtttcctaca ggagtcgaag
     1261 tcccttcata gttatacggt gcgtcagtaa tagtttttcc agtagaatat ttcaaatatt
     1321 caaaagctgg tccgtaatct gtgacaggtt tcatcgtaga accaaaatca cgggaagtat
     1381 tcactgctaa gttgttgcct aaagttacgt cttctgcgat gtgacgtccg ccaatctggg
     1441 cttttacttt cccggtattg gtgtcgataa gggtggaggc aacttgcatc tcgtcgtctg
     1501 ggtaagagac atactgatcc gtgttgacga tatcatatag tttcttttgg gcgtccagat
     1561 ccaaattcgt ataaatctct aaaccgtctg tgtagacatt cttatctgtt ttttcttgta
     1621 cttcattgat gacttctttg acataattat cgacgatctt agtattgtca tcggattgag
     1681 ttagttcttg cagaccatcc gttaccggta cattaacagc ttgatcatat tctgtttgag
     1741 agattttttc gttttgaagc atcgtataca aaacagtatc tcgtcttttc ttcgcctgat
     1801 ctggataaac ataaggatca taggcggaag gggcttgagg catcccagca agcaatgctg
     1861 tttgcggtaa agaaagttct gataatttct taccaaagta catctcagaa gctgtctcca
     1921 tcccgtacaa gccgtttgac atatacactt tatttacgta gtaggtcaag atctcttgtt
     1981 tcgatttttt ttgttccaat cttacagcca tccacgcttc ttgggccttg cgtttcagag
     2041 tctgatcctc ggcactggtg gagaagaagg aaagtttgat caattgctgt gtcaaagtac
     2101 ttccgccttg cagtccgcca gaggtaaagt tagagagggc agaaccgatg atccgaatag
     2161 ggtcaacccc gatgtgttta taaaaacggc gatcttctac cgacacgatc gcatcttcta
     2221 atgttttcgg taattcattc gcagaaattt tttcacgctt ttctgcgcct aaatcttcga
     2281 atagttcgcc atcttgtgta tatagtttgg aagatacggt agcgtctaat tttttatccg
     2341 tcaattcggg cgcatctttt gcgtaatacc agaaaagtcc tacacctgct aaaaagagga
     2401 tacagaaaaa tgagagtatt cctaaaagga tcttgataaa taatcctttt ttatgtgttc
     2461 cagaggactt tccagaacct ttgccagaat tcttcttcac actcttttta ggagtaggct
     2521 gtttctgtcg tctagagctt ctagtttgtt catttgccat tcttgctttc tccttcaata
     2581 gataatgttg ataaatagaa gtcaaccgcg tccaaataag gaatccgcgg agaaatacca
     2641 gttttgatct ctattcctag ttcttcgatc aaagataaag gtagtgattt gcgtcctttt
     2701 tccttttggg cgtcccagta agcaatcaaa tgaacactat tcaaataaaa acagcgcttc
     2761 aaagacgaaa accataacaa gacaaagcag accccatttt gttcgaggca tgctttcata
     2821 tgttcgattt gatgggcatg gaagttttta aagggaaaag atgttttgtt cttggtttct
     2881 tttgcttcaa agtccaagta aaagcctcga tagacgccat tatagtctgt agttgaggct
     2941 tgggtaaagt aggcttcctt gatcacagca gcacttctct taggatagtc tactttcacg
     3001 atttggatag gggtaggctt tttatggata accgccaacc cgcgggaaag ataatattta
     3061 ttactttcat tgattgcttc ttcaaagcgc atcccccgat tgccgaattc agtgatctga
     3121 tttctttttt cttgaatttt ttcgacggga gaggcattct tataataagg ttggccattt
     3181 ggatagcgca ataccatctt taatcacact cctaggccac attatagcaa aaaagcggat
     3241 agaaagaaaa ggaatctgag atggataagt caaaagtaat ctatgtgtct gggtatcgaa
     3301 gtttcgaact cggtgttttt aaagaaaatg atcctaaaat ccaagttgtt aaaaatgttt
     3361 taaaaaaaga actccatcag ttagcagaag aagggctgga atgggtactt gtcagtggga
     3421 atttgggcgt cgaactatgg gctgtgcaaa tagtagcgga attgaaagaa gaatatccgg
     3481 aactatctct tggtattttg tatccttttt ctgattttgg taataactgg aatgaaggga
     3541 accaagaaaa aaaacaatta gcagaacaat tagcagatta tgtcgaagct gttagccatc
     3601 aaccctacca atcacctagt cagctaaaga atcacactcg ttttatactt gagcatacag
     3661 tgggctgtct gctgatctat gatgaagaat tccctggaaa aacgaagtat ttttttgaag
     3721 atgcacagaa ttaccaagca gatcatccct acgaaattcg gctgatcagt atggacgatc
     3781 ttcaaaattt tagtgaataa tcgatattgt gtttgattta tcaacttttt tcctatacaa
     3841 tgaaaaaggt agaaatgtta ttacgtaaat gaaaactaga ggtgtaaata tggcgaattt
     3901 ggtttatagt cctaaagaca ttttacaaca agaattcaaa acaaaaatgc gcggctatga
     3961 tcccgttgaa gttgatgaat tcttagacaa tatcatcaaa gattatgaag cttacagcaa
     4021 agaactcctt gctttacaag aagaaaacga tcgattgagt gcaaaagtag ctcaattatc
     4081 taaaactcaa ggagcagctc aaacacgcgt gcaacaaaca gaggcaccaa agagcgcagc
     4141 agtgaccaat tttgatatct taaaacgctt gtcaaatctt gaacgtgaag tatttggtaa
     4201 aaagcttgat caacaagcaa gtgcagtcaa accagcacaa cctaatccaa ataactacac
     4261 aaatgcagac acaagcttag atgataatga aaaaacacgt caattttaat ttttgattaa
     4321 tgaatttgcg gttttcgggt aatcgcggtc tgacttgatc aggctgagga aagtccatgc
     4381 tcgcacaggc tgtgatgcct gtagtgttcg tgcttagcga aaccataagc taaggtactc
     4441 tttggagtaa cggcaggaaa acaagctaag gcttcggcta tgcttaagta tccttgaaag
     4501 tgccacagtg acgaagcggc tggggaaacc catccggtgg aacgcggtaa acccctcgag
     4561 cgagcaaccc aaacaatggt aggggcgctt tttgtctgga agaagaacag gacaaaaagg
     4621 cagtataact gcagatagat ggttaccgct ttgttttttc tccctggaaa agcaaagtac
     4681 agaacatggc ttatagaaaa ccgcaaacat cagggaatta ggtcgtgaca aaagtaatgc
     4741 gttataagaa acaagaaaaa catttgaaag ctatttttca atacttcaga tgaatcgttc
     4801 ttattacaaa acaaactttt gtatcaccgg ttattcggaa tttgagtaaa agtgtggggt
     4861 aggactgatg tcccacctcc tttttttata aataaggaga ttgtgtatgg aaaaaaacag
     4921 gaagttcaac ctcgtcgcaa ctgcggcaag cgggctagaa gcactggttg gtaaagaatt
     4981 aagagatctt ggaatcgaat gccaagttga gaacggacgt gcacgttttg aaggaacaat
     5041 ggaaacgatc gctacagcta atttatggtt acgtacagca gatcgagtaa aaatcgtggt
     5101 tggggaattc gatgcgtata cgtttgatga attgtttgaa aacgtaaaag cattgccttg
     5161 ggaagatttt cttccactag atgcagcttt tcctgtagct ggaaaatcga tcaagtcgaa
     5221 actatatagc acacctgatt gccaagcgat cacaaaaaaa gcaatcgttg aacgtctccg
     5281 tacctattat caccgtccag cttctgtacc actgacggaa actggcgcac attttcagtt
     5341 ggaagtagct ttgctgaaag accatgtgat ggtaactctg gatacgacag gaccaagttt
     5401 attcaaacgt ggttatcgct tagaaaaagg tggagcacca ttgaaagaaa acatggcagc
     5461 agcacttatt atgttgacaa attggcgaaa agaccgtcca ttttatgatc ctgtatgtgg
     5521 ttcaggtacg ctttgtattg aagcagcctt aataggccat aatatcgcac caggattcaa
     5581 tcgtgagttt gcttgcgaat cttgggattg gttcagtcaa gaaatcatgg aaaatgttcg
     5641 atcagcagcc gaagaaaaag cagattatga tattgaatta gatattactg gttcagatat
     5701 caatggacgg atgattgaaa ttgccaaagc caatgcagaa gaaatcggat taggctcttc
     5761 gattaccttt aagcaacaag cagtcaaaga cttcaaaacg gataaagaat acggggtcat
     5821 cgtggctaat ccgccttacg gagaacgtct aggagaagaa gaaactgttc ggaaattata
     5881 taaagaaatg ggcgatgtat tccgtccatt aaaaacatgg agtaaatata tcttgactag
     5941 cgacttagcg tttgaggaat tttacggaca aaaagcaacc aaaaaaagaa aattgtacaa
     6001 tggtgcattg cgtacggatc tattccagta ttggggaact cgtccgccta gaaaaaaaga
     6061 gaaagacgaa taatcttact aattgattca ttcaattgga tgtttggagg aatagggaat
     6121 gaaagaaaaa gagtttttac aagaagtaaa agaaatcaat cttctacaac aagctttagg
     6181 aatcctcgat tgggatacac aaacaggtat gcctgaaaaa gctagtgcat accgaagtga
     6241 agtagacagc tatctttaca gcatttattt ttcaaaaaaa atcggtccaa aaatcaaaga
     6301 agccattcgc tatttttctg aacatccgga agaattgtcg gaagttggaa ctgccgtttt
     6361 tgccaaagta aaagaagagt atgatttgga acatcgcgta ccggaagatc ttatgagaaa
     6421 atatacagct gccgtttctt cagcacacgg acaatggcaa aaagctagag aaacacaaca
     6481 attttctgat tttcaacacg cattgaatca aaatattgaa ttaacaaaac agttgatacc
     6541 ttactggaga aaagatgaaa agacgaatta tgatgttttg ctgaatcaat acgaacccgg
     6601 gatgactgtc gaaatccttg ataaggtatt tgatcagctt aaagaaggaa ttctttcgat
     6661 ccgccgtacg ctccaagaaa aagggactga acctgaaaca gactttttaa atcggagaat
     6721 gacgaaaaaa caacagaaga gatttgtagt gaaagtaatc gaacaacttg gttatgattt
     6781 tagtcgtggt cgtttagatg acactgtcca cccatttatg actggaatta atccgaatga
     6841 tgcacgaatc acgacacgat ggaacgaaac agatttcaaa atggctgttt ttggagtgat
     6901 ccacgaggct ggtcatggta tgtatgaaca agacatcgat gataaatatg cgtatacacc
     6961 ggtttatgaa ggtacatcaa tgggtatcca tgaatcacaa tcgttgttta acgagatcat
     7021 tattggaagt agtcgcagct tctggaaaaa gcaatatcct ttctttcaag aatgtgcaga
     7081 aggaacattc gatgatattc cttttgacaa attttatcgt tcattgaacc atacaaaatc
     7141 aagtttggtt cgtatcgagg cagacagttt gacttaccct ctccatatca tcattcgtta
     7201 tgaaatcgaa aaaatgattt tcaatgaagg ggtagatgtt gctgaactac ctgaaatatg
     7261 gaatcaaaaa tatgaagagt atctgggaat acgaccaaca aatgatgtgg aaggcatcct
     7321 acaagacgtt cattggtcag gtgcaagctt tggttatttt ccctcttatg cattagggta
     7381 catgtatgcg gcacaattac ttcattcttt gcagcaagaa atagatgtgg aaaatgttct
     7441 agcgtcagac gattattctc caatcaaaaa ctggatgtca gagcatatcc atcaatatgg
     7501 tgcatctaga aaaccaaatc aattaatcat ggatgcaaca catgaaccgc ttaatccgca
     7561 atacctgatc gactatatga aagacctgta ttttaatgta tatcaggtaa aataaaatac
     7621 aatttacaaa gatagctgaa attcatctaa tctatagatg ttttcagcta tttacttaag
     7681 aaagaagtgg aacgaatgga taaattacaa gtgatcgctg attatagtat ggaccaatta
     7741 gcgcatgatc aaacaggaca tgggagcgat cataccaaaa gagttgtcaa gttagctgaa
     7801 aaaattttgg agacggaacc gcaggcagac cgctttgtga cgcttgcagc agcttaccta
     7861 catgatacga ttgatgataa agtcgttaaa aatgagaacg aggcaaagca acagcttcgt
     7921 gtattccttc gctctttgcc aatagtagag gaacagatct cgatgatttt ttccattatc
     7981 gaaaatatgt ccttttctaa aaatctctct gaagcggccg aactatctct ggaaggtaag
     8041 atcgttcaag acgctgatcg tatcgaagca ttaggagcaa tcggcatttt gcgtactgct
     8101 tatttcggcg gtggacatgg gcacccgatt tttgatcctg aactgtatcc gcaaacattc
     8161 aaggacaaag aaaattaccg aaaaggcacg acagtgatca atcattttta tgaaaaactg
     8221 ttcttgctgt ctgataaaat gaatactgat tacggatatg aagaagccaa acgaagagaa
     8281 accttcatgc gtgaattctt agaagaattt tttctggaat ggtctattga cgatgaaaat
     8341 tttactccag acaattggat caaaaataac aaaaaatgat ggaggttgtg acagaagtgg
     8401 tcagctccaa gatatttcgg atcattttcc gtaaaagtta cttctggaac actgtttacc
     8461 aagaggtcgt gagcctgccg ttcagctccg agtcacaagg aacaatttca aaaaatagct
     8521 tctcgtgttt gatgaatatt gtaacttgtg attcggaatt ggcaggagaa caccgtttat
     8581 tcggaagaat aggactgtga caagactttt gtcacagtcc ttttttcgaa ttcaacaagt
     8641 aaaactttta agaaatattc agagagtaaa attaaaaatt gctgataaac gaagatggaa
     8701 attttatgta tttttattgg tctatgcaat aaataaatta aatattcaga atacaattga
     8761 ttttaacgaa agggcttcta tcaatggttg tgttgtattt atctgcccag gtagagtaaa
     8821 atagatcatt atctaaaagg taattgttca tactatataa gtcatttgaa agtaaaaaga
     8881 tactgtaaat aaaaaatgtg gttaaataat tctcataatc agtgtaagcg ttatcaaaaa
     8941 tatagcatac ttgctttgta gatgaatgac acgatatatg tttggtatgt tctgcaagac
     9001 agatgaatat ggaggtggag acaatgtctt ccaaagaact aacaatgtat ttagagaaag
     9061 aattggatca actgaaagaa aaggggttat acaatacgat cgacatacta gaaagtgaaa
     9121 atggtgcttg tatcatcgta gacggaaaaa agatgatcaa tctagcatcc aacaattatc
     9181 ttggatttgc caatcgcgaa gaattgaaaa aagcatgtat tgaagcaacc gaaacttatg
     9241 gtgtaggagc aggagcagtc cgaacgatca atggttcgct gaaaatccat cagcaattag
     9301 aagaaaaaat cgcagaattc aaagggacag aagcagcaat cgcctttcaa tctggattca
     9361 actgcaatat gggcgctatc tctgcagtaa tgacaaaaga agatgctatc ttatcggatg
     9421 aattaaacca tgcatctatt attgacggtt gccgcttatc cggagcaaaa atcatccgaa
     9481 ttaaacatca agatatgaaa gaccttgaga aaaaagcaaa ggaagcgata gaaagtaaga
     9541 aatataaaaa aatcatgtat atcacagatg gtgttttctc catggatgga gacattgcac
     9601 gattgccgga aattattccg attgttgaaa aatatggact gatcacctac gtcgatgatg
     9661 cgcacggctc aggcgtaact ggaaaaggtg caggaaccgt caagcatttt ggattaagcg
     9721 acaaaatcga tatgcaaatg ggcacgttat cgaaagctat aggtgttgtt gggggttatg
     9781 tagctggatc gaaaacacta atcgactggt tgaaagctag aagtcggcca ttcctatttt
     9841 ctacttcgct aactccaggt gcagctgcag ctgcattggc ttcgattact ttgatgcaag
     9901 aacatccgga acttgtcgaa aaagtttggg aaaatgctaa ttattttaaa gaagaattga
     9961 aaaaagtagg ctataacatc ggtatgtctg aaacacctat tactccagtc attttgggag
    10021 acgaaaaagt gactcagact ttctctaaga agctgatcga acatgggatt tatgctaaac
    10081 caatcgttta tccaactgtg cctcttggga caggcaggat cagaaatatg ccgactgcgg
    10141 aacatacgag ggaaatgctt gatgaagcag tagcagttta tcaaaaaata ggaaaagaaa
    10201 tggaaattat ttaactaaat ttagtttttt cgtctattta aaattagaga acatgttcac
    10261 gcatatattg ttaatcatct gaagatagta gtaaaaaaga tcagggagga tcatcatgaa
    10321 aaaagtattg gtgacaggat gtttaggaca aattggttca gagttgatca cacggctacg
    10381 tcaagaacta ggcagtgaaa atgtcattgc gacagatata cgcatgcctg aagtcattga
    10441 gaaaaacgat atctttgaga tcctagatgt aatggattac gaaaaaatgc gcagcatagc
    10501 agaacaatat caagtggaca cattgattca tttggctgct cttttgtcag ctgttgcaga
    10561 agcaaaacca caattagcct ggaatttgaa cgtgactggg ctggtcaatg cattggaagt
    10621 cgctagagaa ttccagctga aattttttac gccaagttca atcggcgcgt ttggtccaaa
    10681 tactccaaaa gatcataccc cacaagatac tcttcagcgt cctacaacca tgtatggggt
    10741 aacgaaggta gctggagaac tactatgtga ttattattat gtgaaatatg gcgtagatac
    10801 tcgtggtgtc cgtttccctg gattgatttc ttatgcaacc ttgccaggag gcggtacgac
    10861 agactacgca gtcgatattt attattcagc aatcaaagaa ggaaagtaca ctagtttcat
    10921 tgatcaaggt acatcaatgg atatgatgta tatgccagat gcgatcgatg ccattataaa
    10981 actcatgaat gcagatcctg atcgattggt tcatcgaaat gccttcaata tatcggcgat
    11041 gtcttttgac ccggaagaga tcaaagcatc tattcaaaaa atcataccag attttgaaat
    11101 ggattatgat gtggatcctg tacgtcagtc aatagcagaa tcttggccaa attcactaga
    11161 tgtttcttgt gcgcaagagg aatggggctt ttcgccgaaa tacgacttag atgtgatgac
    11221 aaaggatatg ttggaaaaac tgaaagaaaa attggtagag acggtataaa acaagaacca
    11281 catccaaaaa gttttttata cttttgggat tggctatggt atgaaagggg acaagggatg
    11341 gaagaatatc aagccacgcc agtaaaagaa gtaaaaatag aaaatgaatt aaaacgtacg
    11401 atgggatttt ttactgcttt atcgactgtt atgggcacag tgatcggtgc aggggtattt
    11461 tttaaagccg caagcgtagc agaagttaca ggatccgcca gtttgcatat gttttcttgg
    11521 tttctcggtg gtatgatttc tgtctgtgca ggactaacag gagctgaact ggcagctgct
    11581 attccagaaa caggagggat gattaaatat atcgaacgga tttacggaaa tacagcagct
    11641 tttttattgg gctgggcgca agtcgtgatt tattttccag caaatgtagc agccttgtcg
    11701 atcattttcg gtacacagtt cgtcaatcta tttggtcttt cccagtcaat gattgttccc
    11761 gtagctgtta cagctgctgt atcgatcctg ttgatcaatt ttttaggttc taaagctgga
    11821 ggggcttttc agtcgattac gcttgtctgc aagcttattc ctttgtttgt gatcgtcatt
    11881 ttcggtttgt tccgacaaga aggagtagac ttccaactgt ttccgatcca agcaggagaa
    11941 aatctttcat ttttctcagc cttgggagcg ggcttgctag caacgatgtt cgcatacgac
    12001 ggttggatac acgtaggaaa tatttcggga gaattgaaaa agccggcgaa agatttgcca
    12061 aaagcaattt ctttaggaat catcggaatc atgatcgttt atttattagt aaatgcggtg
    12121 ttcttaagaa ccgcatctat cgacggagtc gcaggaaaca gcaatgcggc aagtgacgtt
    12181 gcaaaaatga tttttggcgg cttcggtgga agactggtca cagttggtat cttgatttct
    12241 gtttatggaa ccatcaatgg ctataccctt actggaatgc gtctccctta tgttatggca
    12301 aaagaaaaca acctgccttt tagcaagctc tttgctaaac ttcatgataa aactaaagtt
    12361 cctgtagccg caggtatctt agagctagtc atcgctattg gcatgatgat gatcggtggt
    12421 tttgatacac taacggatat gctgatcttt gtcatttgga ttttttatac gatggtcttt
    12481 gttggtgtca ttcttcttcg taaaaaagaa cctgatttgt ttcgaccata caaagttcca
    12541 atgtatccgt ttattccact agtagcaatc attggaggga cttttatcgt atcaagcacg
    12601 cttatcactc agaccttttt agcttcgatg gggatagtat tgacacttgc tggtatcccg
    12661 atttatctct atttaaaacg tagatagcta aaacattcga attttattag aactgcactt
    12721 ctctgccaaa caaaatattt tatttaaatg aggagtacgt ggaagaaatg tcaaaagtac
    12781 tcgttaattt tgatcacgta aatgtcgttt tgtctacgat gaaatagcaa agtaaaagac
    12841 agaaatcggg ctcaaccgat ttctgtcttt tactttgatt tgcgtaactg aactattcgg
    12901 cgtacgccgc gataaatatt taataaaacc aagcaaccag aaatgaaaat gggaagccaa
    12961 atcagataga cagggtagcc aagcacttta gacaaagcat gtggagtcat tttatccatt
    13021 tcaagaagtg cttgctgcca agtctgaaga ttgaagacta gcatgaaacc cgaaataatc
    13081 aggagaataa atcctaaata atgaattgta gttctttttc ttacataagc gatcgtagca
    13141 taaaggaaca gtaaaaaaat aggtacgcca aaaacaaagt acatattcag catctcgaca
    13201 ccccctttgt gtttatctta ctttactttg aaagaaatga gaaataatag cctcgttact
    13261 tgcttgaaac agcaaaggac tgctaagata agagagatac aaagaatcaa ggaggagtca
    13321 tatggcaagt tatatcgaac cgattcgttt tgcgctgatt gtttttccat ttttagcctt
    13381 agcgatctca tctgtttttt ttatttatga atatcgaaag tatggaacat ttctagtaac
    13441 aagggcagtt attttgtatt ctttcgtttt ttatttattg tgtgcgtatt ttttggtgat
    13501 cttaccactt ccggcaaagg agacggttgc ccaaatgaca gggccaaaga tggagctgca
    13561 tttatttgct tcatggcaac atttcagaga caacacggta ttggtcttgt ctgaacctag
    13621 tacctacctg ccggcaatga agcagagtgt atttttggaa ccagctttta atgttttatt
    13681 actcatacca tttggcattt atttaagata ttatttccgg ctttctttta tgaaaacgat
    13741 gattgctagt ttctgccttt ctctattttt tgaattgacg cagctaagtg gactttattt
    13801 tatctaccca agaccatacc gcttatttga tgtgaatgat ttagtgacaa atacattggg
    13861 tggaatcatt ggttttgtcg ttacgccgtt atttacattt atgcttccta caagaagacg
    13921 aatggatgaa ctttcttatg aaaaaggaaa gactgtctca ttgactcgac gcttggtcgc
    13981 ttatttgatc gactggacat ttttatctct tattaatttg gtagcggcga ttttctttaa
    14041 aattgcgttc gattcagggg cagttacttc tcatatctgg tggcttttat tagaagtagg
    14101 ggtatacttt atgattgtac cgtttataac caatggatgg acacttggca aaaaaattgt
    14161 gcgtatccgt ttggtggaag aagaccgcga acgaatttct ttaaaagcat tgatgatccg
    14221 ctacgggtat ctgtactttc ttttttatgg tttaagctgg ttggggcagt tgcttcagca
    14281 aatcatgaat tcgacaacga atacggtgtt gcagatgagt gcagtattca tttctctcgg
    14341 tgtcttgtta ctacaaggta tttttatttt aaatatcttg ctatcatttt tgagaaaaaa
    14401 acggattctg ttttatgaaa aagctagtca cacatatact gttagcacga ttggccaaac
    14461 aaaggatgat gattctcgaa attagctgag caaaaacgct ttgtctgtgt tataatgcca
    14521 aagtcgtgat aatttaaaga gaagaggtgc acgaatgaat aattttgata tattccgctt
    14581 caaaccgttt atcaatgaag cgttaaccgc aaagggcttc aaacagccca cagaggtcca
    14641 ggaacgtttg attcctttaa ttgcaaaagg aaaaaatatc attgggcaat ctcaaactgg
    14701 tacaggaaaa acacatacgt ttttgttgcc tttgtttgaa aaaatcaatg cagaaaaaga
    14761 ggaagtacaa gttgttatta cagctccaag tagggaacta gctgcacaga tctatgaagc
    14821 agctacacaa atcgctaaat tttctacacc ggagatccgt gtctcgaatt ttgtcggcgg
    14881 tacagacaaa cagcgtcaat taaatcgtct gcatcaccaa cagccacagg ttgtagtagg
    14941 cacgcctggt agaatattag acatgatgaa tgaacaagcg ctgaagattc acaccgcatt
    15001 tgcatttgtt gtcgatgaag cggatatgac attggatatg ggttttttaa atgaagtaga
    15061 ccaaattgca agtcgattac cagaaaagct gcagttttta gttttttctg ccactattcc
    15121 agaaaaactt cgtccattct tacgaaaata catggaaaat ccaatcattg aagaaatcaa
    15181 accaaaagca gttatttctg aagacattga aaattggctg atttcaacaa aaggtaaaga
    15241 tgagaatcaa atgatttatc aactgctgac aattggtcat ccttatttag caatcgtatt
    15301 tgccaataca aaacagcatg tggatgaaat tgccgattac ttgaaaaacc aaggcttgaa
    15361 agtagcaaag attcatggag acatcacccc aagagagaga aaacgagtga tgcgacaagt
    15421 acagaatctg gagtatcaat atgttgtagc gactgattta gctgctagag ggatcgatat
    15481 cgaaggtgtc tctcatgtta ttaatgcaga aattccagaa gatctagact tctttatcca
    15541 tcgtgtcgga cgtactggac gtaatggctt aaaaggaacg gccattacgt tatattcgcc
    15601 aaatgatgag caagccattg atgcgataga aaaattgggc gtctcatttg aaccaaaaga
    15661 aatcaaaaac ggggaaatcg tcaaaacata cgatcgtaat agacgtgcca aacgagaaaa
    15721 atcaaaagat gtattagatc ccacgctgat tggtcttgtg aagaagaaaa agaaaaagat
    15781 caagcctggt tataagaaaa aaatcgattg ggcaattgca gaaaaaaata aaaaagaacg
    15841 caaaatcgaa cgcagacagc aaagccgtac tgctcgtaaa aataagaaaa attcacataa
    15901 ataaaaaaca agaggttgtg acaaaaggcg tgtcacaatc tcttgttttt aagctagact
    15961 ttttttcatt ccgatacaaa gtacttcatc ttcaataaaa ggttctgtgc aagtatgata
    16021 cccttgtttt tcataaaaat ctacagccgt catttcagct actacataac tagtctgaca
    16081 accttctgaa accgcccgtt tttcagcagc ttcaagcaac tgaccgccta acccttttcc
    16141 tcggtattct tttgctacgc aaaatcgttc cggcttgaag caagtaggtg ttttcttttc
    16201 ataacgcaaa gtagctactg gtcttttttg atcaagaaat agataatagg tttgttgcgg
    16261 gatatctttt ccgtcgaatt ctagttctgc aggaatccct tgttctaata caaaaacttg
    16321 atatctcaaa aagcagctag ctgcgcgtat ccattcatcc gctccaacta tttttttcat
    16381 catattcctc cttgtcatga atagcagaga tgtattgatt cctttattct tatcgtacca
    16441 aaaatcgtgg agtatgacca aaaggaaatg aaaataaaaa ttgagatatt gacagtgatc
    16501 aatagaatgc gtatactaat taagtacgaa aaggatatga tttgtttttt aagactcaca
    16561 gaaaattctt tcttggctga aaaaagaata gtcactaatc aaattgctcc ctttttctat
    16621 tttggtagaa ataccgacgc agctcagcgt tatcgagttt aagaggtaat gattacacat
    16681 cattgcaatc aaggtggtac cgcgagtttt cgtccttggc tgtgatggtg tttttttgta
    16741 tttaatttat agaaagcagg taatgacatg aaacaattaa gtagtgcaca agttcgacaa
    16801 atgtttcttg atttcttcca atctaaagga catacgattg aacctagtgc gtctttgatc
    16861 cccgtgaacg atccaacctt attgtggatc aactctggtg ttgcaacatt gaaaaaatat
    16921 tttgatggtt cagttgtgcc agaaaatcca agaatcacaa atgcccaaaa atctattcga
    16981 acaaatgata tcgaaaatgt cggtaaaaca gcacggcacc atacgatgtt tgagatgctg
    17041 ggtaattttt cgattggtga ctatttcaaa aatgaagcca tccactgggc atgggaattt
    17101 ttgactgatc caaaatggat ggcattcgat ccagaaaaac tttatgtaac agtttatcca
    17161 aaagatacag aagcaaaacg catctggcat gaagaagttg gcctgccact agatcacatt
    17221 attgatgtag aagacaactt ctgggatatc ggtgcaggcc catgtggtcc ggatacggaa
    17281 attttctatg accgtggaga agcttataac gatttagcag aagacgatcc tgaaaactat
    17341 ccaggaggag aaaatgaacg ttatttagaa atctggaact tagtattttc agaattcaac
    17401 cataagcctg ataacacatt tgaaccactt cctcataaaa acattgatac ggggatggga
    17461 ttggaacgca tggtctcaat cgttcaagat gcgtcaacga actttgagac agacttgttt
    17521 atgcctatta tccacgccgt agaaaagcta agcaaacaag taaaatatgg tgaaaaccca
    17581 gtaacagata tttcttacaa agtaatcgca gatcatattc gcgccctttc atttgctatt
    17641 ggcgacggtg cattgccatc gaatgaaggg agagggtatg tcttacgccg tttgcttcgt
    17701 cgtgctgtca tgcatgggaa aaaattaggt attgaacaag cattcttata tcgtttagtc
    17761 cctgttgttg gggaaatcat ggtaagttat tatcctgaag tacttcaaca aaaagaattt
    17821 attgaaaaag ttatccgtac agaagaagaa cgtttcaatg aaacaatcaa tgaaggtttg
    17881 gaaatcttaa atcaagtgat ccaagaagta aaaaacgaaa acgggacgac tctagcagga
    17941 aaagacatct tcaaactata cgacacatat ggcttcccgg tagaattaac ggaagaggtg
    18001 gcagaagatg aaggactgaa agttgatcat gctggttttg aaaaagaaat ggatgcacaa
    18061 cgcgaacgtg ctcgctctgc ccgcagtact gaaaaatcaa tgggggtaca atctgcttta
    18121 ttgacagata tcaaagtaga aagtaaattc attggataca cagaacaaga atcagatagt
    18181 caattattgg tcatcgttaa aggtgagtct cttttgaatg aagtcgcaga aggtgaagct
    18241 caaatgatct ttgatcaaac acctttctat gcagaaatgg gtgggcaagt agcggatcaa
    18301 ggatggatca tgaatgaagt aggcgaaatc gtagcagttg ttacagatgt tcaaaaagcg
    18361 cctaatggtc aatttatgca caccgtagaa gtaaaagcac caatgagtga aggacaaacc
    18421 tatcatctat tgatcgatca atcgatgcgc aatcggatca tcaaaaacca tacagcaacc
    18481 catctactgc acaaggcatt gaaagaagta cttggcgagc atgcaaatca agctggttct
    18541 ttagtcgctc ctggacattt acgattcgac tttacccact ttggtcaaat cacttcagat
    18601 gaattagctg aaatggaaag aatcgtcaac gaaaaaatct gggaagctat cccagttgaa
    18661 acaattgaaa cagatatcga tacagccaaa aacatgggtg caatggcttt gtttggtgaa
    18721 aaatacggac atgacgttcg agtagtaaat attggcggct ggtcaatcga actgtgcgga
    18781 ggcacacatg tagcaaatac agaaaacatc ggaatcttca aaatcgtttc tgaatcagga
    18841 attggtgcag gcgtgcgacg aatcgaggct gtaacaagta aagaagcgta cgaactactt
    18901 gaagaagaag aaaaacaact aaaacaagta gcaacaattg ttaaatcacc tcaattgaaa
    18961 gaagttgttt caaaagcgga acaattgcaa cagcaagtaa gagagctaca aaaggaaaat
    19021 gaacaattag ctagcaagtt agcgaatcaa caagctggtg atgtattcaa agatccagaa
    19081 acgatcaatg gaaaaacaat cattgcagca caagtaaaag tgaaagatat gaatcagctg
    19141 cgtcaattag ctgaccaatg gaaacaaaaa aatatttcag atattcttgt tttagcaaac
    19201 gaacaagaag gaaaagtcag tctgctagcg gcaatgacga aagaatcaaa tgaagctgga
    19261 ctaaaagctg gcgacttgat taaagcaatc gcaccgaaag taggcggtgg cggtggtgga
    19321 cgtccagaca tggcacaagc tggcgggaaa aatcctgctg gtattccaga tgcacttaaa
    19381 gctgcgaaag aatgggttgc gcaacaatag ggcgtttacg tttgacagaa aaacaaaagt
    19441 tcaaacattg aacgaaacat gattatgggg gcgaagacaa acaagtcttg ccctcatact
    19501 tttttcaatt ggatggcgca tctaatcaaa tggtcggatt cggatggtct ttagttgacg
    19561 aaagtgacag aaatttttac gattagtata aataaaaagg ggtgaaaaaa tgaatagtag
    19621 aaataagacc tttcgactag tcttacgtgc gatcttgtta gcaatcatca tcgtacaagc
    19681 gatggttcct tggctggggt ttattccatt aggattcatt agtttaacaa ttatccatat
    19741 cacagtaatt attgctgcag tagtattagg cccaaaagat ggcatggtga ttggcttgtt
    19801 ttggggaatc gctacaattg ttcgtgccta tgccatgcca acaacgccat ttgatacatt
    19861 agtgttcacg aatccgatta tctcggtagt tcccagagta cttgttggct tagtagcagg
    19921 cttggttttc cactggattt atcaaaaaaa taaatcaata acgatcagct cagcctttgc
    19981 aggggtactg ggttcattgg tcaatacagt cttagtgtta ggattcatgg gattatttta
    20041 tacaggtgca acggcagatg cctatggtgt agactctgct cttttattta aaaccttagc
    20101 aggggttgca gcaatcaatg ggataccaga agcaataggt gcgggaatca taacaccatt
    20161 actagcaaaa gcgttatttg cagcaacacc gttgaaacca gaataatgac catcttttaa
    20221 tagtaatagg acgcacgata gatagaaaaa ggaagagagg agaggaaaac aatcgtcctc
    20281 tccatctttt tacctgagca acaagcaaac tagaaggggt aaaaaacttg gctaagaaac
    20341 aagaagaaaa aaagaaacaa ataccgcaac agcggacaaa aaaaggagta ctagcgggta
    20401 tcttaggatt ggttacgaat ctgatcttgt ttgtctctaa gctaatgatc gggctttttt
    20461 caggaagtgt ctccattatg gctgatgcag tgaacagtct atcagatact gcttcatcca
    20521 tattgacttt gatcggattt aaaattgctg caaagccagc agatcaggaa catccttatg
    20581 gacacgaacg cttcgaatat atcagtgggt tatttgtttc gatcatcatt acttatgtag
    20641 gatttcaatt tttagagtct tctgctgaaa aaatcatcca tccagaaaat gtctctttaa
    20701 cacccatcgt cttttttgtc ctaatcattt ccatcttttt gaaatttgca caaggcaaaa
    20761 tgtatcaggt tattgcgcaa tccattgatt ctgaaacatt gagagctaca gcaacagata
    20821 gttataatga tgtctttact acactggctg tattgctttc agcagcaatc gaatgggtaa
    20881 ctggatggcg tattgatgga tatataggtt ttgctttagc attatatatc ctttatagtg
    20941 gattgacgat gcttcgaaat tttgtatacg aactattagg aagtcgccca acgccagcag
    21001 agattgaaga aatggaagag catttaaatg cttgcgatga tatacttggc tatcatgatt
    21061 tattggtgca taactatgga cccaataaac gctttgcatc agtccacatc gaagtagatg
    21121 aatcaatgaa tttgaatgtg gctcataaga ttattgatct gatagaaaaa gactttaaaa
    21181 agtctttgga tgtggaactt gtctgtcact tggatccaat gcctgttgat aacgaaaaat
    21241 actataatat cttgcggcag ttaaagcaaa ttcttcttga aacaggtaca gggcttaata
    21301 ttcatgattt tagagtaatt catcaaggaa aaacgctgca atttgatgta gtggtccctg
    21361 agaaattcca gtttgttgat acagaactag aaagaatctt acgtagtcaa atagaagaaa
    21421 aaatcggaga ttaccaattg gaaattacgt ttgatcacaa ttatctttta tattaaatga
    21481 gaaactataa aaagactcct taaagttttt aatttaaaga gtcttttttt cttttaattg
    21541 tttatggaga gcctgacgtg caagattatc tgctccttta tttttgtttt cgggcaacca
    21601 ttgcaggatc aataaagaaa attcggatag aagttcttgg attctttgaa ggtagggaag
    21661 gaagagctcg ttcttcgttg cgtttttgtc gaccgtagaa actagtgttt tgctgtctga
    21721 aaaacagaaa atcgtttcat tctgtaagtc gtttttcttt agaaattcta aagtttttaa
    21781 aaagacttcg aactctgctt gatggtttgt ccctatagaa agcgggatag atagctgttc
    21841 ttgtttacct tgatagacaa tcaagattcc acctccacta ggaccagggt tgccttttgt
    21901 agaggcatca atgtatactt ttaacataaa aagagattct cctttttgtt caaactaatg
    21961 ttatggtaag attatatcat tactgaatcg gtgagaggtt agagaatgaa aaaaaatatt
    22021 cgatggcagc ctgaactggc acatacgatc atctattggt cattgacctt gatcattctg
    22081 ttttttagtc tgattctttc tttagaaaac acaggtcctt attggaaaag caattttgtt
    22141 ttaggtattt ttcttgtttt tctatttcta ggtttgcggc gttcttttat tttaaaatca
    22201 aactctgtga ttatacgtta tgctgctttt tggaaagacc gagaaatatc tgtcggacag
    22261 attcgatctt tgacgccaac taatactggg gtgcaaatga tgttgcaaga tgagaaagcc
    22321 ccatctgttt tcatgatgcg aaaaaaagat caagtagtcc tttttgatca ttttgaaaaa
    22381 aacggaccca aatcagaatc aattccttat ccttatacga aaaaaagaaa aagctaattt
    22441 taaaaatcag aacctaaaaa aacgaatttt tacaggaaaa gaaaagagtg agacgtacgg
    22501 ttcatcccgt ttagcgtctc actctatttg tttatctatt catgcatact agtatcggta
    22561 gtctgaacgt tgcctttctt gacatgggca gcttgtgttc ctcgattccc ttcgatgatc
    22621 tcaaattcga ccacttgatt ttcttccaaa gttttgaatc cctcttcttc aatcgcagta
    22681 aaatgtacaa aaatttcttc tgtgtcatca taactgataa agccgtatcc ttttttgttg
    22741 tcaaaccatt ttacaattcc tgtggtcaaa gcactcacga cctttctaaa gtctgatcat
    22801 agccttcatt ataaagagta tttataagta cgtcaaataa attaaaaacc tgtaatttta
    22861 tgtttttttt ctgaataaaa aaaggtataa ttggcatatc ttaacaaaga agatattttg
    22921 ttgaataaag ttcgtgaaaa ggggtaatca aatgaagaat gatctgcaaa tttcacaaga
    22981 aacagagcta gaaccgattg ttgaagtggc tagtaaatta gatcttaagg aagacgattt
    23041 ggaactgtat gggaaatata aggcgaaaat caatttttct ggaatcaatc gatcaaagga
    23101 taacgctgag gggcatttga tcttagttac ttccatcaat ccaacaccag ctggcgaagg
    23161 aaagtcaacg atcactgtag ggttaggaga cgcgctcaat aaaatcaata aaaaatctgt
    23221 tattgcattg cgagaaccct cacttggtcc tgtgatggga atcaaaggtg gtgctgcagg
    23281 cggaggatat gcacaggtat tgcctatgga agatatcaac ttgcatttta ctggtgacat
    23341 gcatgctatc acaacagcta ataatgcatt atctgctctt ttagataatc atattcatca
    23401 aggaaatgaa ttgaacatcg atgcccgccg agtgatttgg aaacgggtag tcgatctcaa
    23461 tgatcgtgaa ctgcgtaaag taattgttgg cttgggtggg ccaatccaag gtgttccaag
    23521 agaagatggg tttgatatca cagttgccag cgaaatcatg gctatcttgt gtcttgcggc
    23581 agaccttgaa gatttaaagg ctcgtcttgc aaggatcgtt gtcggctata catttgaccg
    23641 tcagcctgtg actgctggag acttgaaagc agaaggcgct ctggcattac tgctgaaaga
    23701 tgcaatcaag ccgaatcttg tacaaacaat ttacggaaca ccagcttttg tccacggggg
    23761 accgtttgcc aatatcgctc atggctgcaa tagtatttta gctaccaaga cagcattgag
    23821 attagcagat tacgttgtca cagaggcagg atttggtgca gatcttggcg gagagaaatt
    23881 tttagatatc aaagtaccta acttgaaaaa agcgccggat gctgtggtga ttgtcgccac
    23941 tgtccgtgca ttgaaaatgc atggcggaat gaaaaaagat gaacttaaaa atgagaatct
    24001 tgatgctttg aaagtaggtt ttgcaaattt aaagcgccat atacgtaata tggaacaata
    24061 tcagctacct gtgatcgtcg caatcaatga attcgtaaca gatacagaca atgaactcac
    24121 actgttggag cacttgtgtg aaaatcaggg gattcttgcc aagagagcga gtgtatgggc
    24181 agatggagca gaaggcggcg ttgatttagc aaaagctgta gtacgattga ttgatagaaa
    24241 agaagctaat tacaagccat tataccgcct agaagaaacg atacaagaaa agacagaaac
    24301 aatcgtcaag aaaatttacg gaggaaatgg tgttgttttc agcaaaaaag cgcaaaaaca
    24361 aatagaagag tttacgaaga atggatggga caaactgccg atttgtatgg caaaaacaca
    24421 gtattcattt tctgatgatc cttcactgct aggtgcaccg gaagatttca ccatcacgat
    24481 ccgcgaaatc atcccaaaat taggggcagg atttttagta gccttgactg gagatgttat
    24541 gacgatgcct ggtttgccta aaaaacctgc tgcattgaat atggacgtga caaatgacgg
    24601 agaagtgttg ggattgtttt aagcttgatt ataatctacc atcgaaggag ctgtattttg
    24661 actacagctt ctttttttag acgataggga attaattttg tttacactag gatcattctt
    24721 tttctttatt gtattttgag ctatactaaa gcataatagg agggtatgat gaaaacgaca
    24781 tttataagaa aaaccggttt ttacggtatg ggtagctcga ttcagctaag aatcaacaat
    24841 gaaaaacgca gtgttctcag tcatgaacaa aagataacgc ttgatctgga agtgcccttt
    24901 acgatgcagg tcacctttta ttggctaaaa agccccgttt atacagtgaa agaagcaaag
    24961 cctgcttatg tgatcacaat gaattacttg attcttcaaa tctatccgtt tttatttcta
    25021 gcaactgggt tgggggccgt tttcctacaa aatttatttt tcagtttttt tgcagttatt
    25081 gtgttgattg cgttcttttt ctatataaaa aaccgggcat atttgatcaa ggaggaaaat
    25141 tatggcaaac tctgatcttt ttttaatcag tttcaatcga atcgaaaaat ggatgagaga
    25201 cgagatggga aatgcacgaa atatgggatt taccgaactt gtcagacgcc tagctcaaag
    25261 aaaacaatta atgatcagaa aatacgaaga tgatctttta cagcttgctc agttaaggaa
    25321 tgcgatcgtc catgatcgga tcgcagtcga ttttgtcatt gcagagccaa atgattggac
    25381 agtcaagcga atccagctga tcgaacaaga actgattcag cctgaaaccg tgctgcctcg
    25441 atttgcaaaa cgtgtaacag gttttgaaca agatcttccg ttacttgaac tgttaaagat
    25501 agtagcagaa aaaaggtatt cacaatttcc gttatacaat aaaggaaaat ttgtagcatt
    25561 gattacttta cgtaatatcg ggttttggct agcaaaagaa agccaaaaag gtccggttga
    25621 tttaacaaat aaacgagcgt tggatttgat catccaaaac ggaaagtaca caaattatca
    25681 ttttgttcca gctaaaacac atatttacga ggttgaagcg atgtttcgtg agcaagggac
    25741 tttggaagcc gtacttatca ctaaagatgg gaatcccgat ggtaatctgc tcgggatcgt
    25801 acgacctcgt gatatttata aacaaataga aaaggattga ttttttgtta attatttatt
    25861 ggatattcag cgctttgcta gtaggagtgg atcagttggt caaatggtgg atcactgata
    25921 atctagcgct tggagagacg aaaaatctta taccaggtat tttgtcactg aatcatattc
    25981 gaaacactgg agccgcatgg agtatgctag aagggaagat gtggttcttt actatagtga
    26041 cactcattgc agtcgttgtg attctcacat tgatgattaa aaatcgtgaa aaaggtaatc
    26101 gctggttcat gattggattg agtttgattc tagctggtgc gattggcaat tttatcgacc
    26161 gtattcgtct tggctatgta gtagatatgt tccagacaga ttttatgagc tttccgattt
    26221 ttaacgtagc agatgtgaca cttgtatgtg gagtgatttg tatgttaatt tatattattt
    26281 tagatgaaaa ggatcaaaga aaaaaatgac agaacttaaa acgacgattc accatgaatc
    26341 tggtaggatc gataaagtat tgactaattt attcaccgat tacagtcgtt cgcaaattca
    26401 gctgtggctg aaaaatggag cggtttctgt caatggacag ccagtcaaag caaattacaa
    26461 agtaaaacaa ggagacgaga ttactgtctt gatccccgaa ccagaaacat tagatgtggt
    26521 cccagaaaat atcccgttga cgatcgttta cgaagatgaa gatgtagcgg tgataaacaa
    26581 acctcaagga atggtcgttc atccttcagc aggtcatacg catggcacaa tggtcaatgc
    26641 gctgatgtac catatcaagg atctttctac gatcaacggc gttattcgtc caggtattgt
    26701 tcaccgaatc gacaaagata cttccggact gctaatggtt gccaaaaatg accaggcaca
    26761 cgaatcatta gctcagcaat taaaagataa gacctctttg cgcaaatatg ttgctttagt
    26821 ccatggagag attcctcacg aaaaaggaac catcaatgcg ccaatcggcc gttcgaagga
    26881 aaaccggaaa atgcaggcga taagagaaga tgggaagtcg gctgtcacac attttactgt
    26941 attggaacgt ttcgaaggat ttactctggt tgaattaaaa ctagaaaccg gaagaacaca
    27001 ccagatccgt gtgcatatga agtatatcgg ttatccgtta gctggagatc cagtatacgg
    27061 accgaagaaa actttgaaag gcaatggtca gtttctccat gcgcaaatgt taggttttac
    27121 gcacccgact acaaacgaaa agatgatctt tgaagcacct cttccagaag tttttgaaaa
    27181 aactttagaa aaattaagaa aaaacattgc attttaattt agataacggt atagtaatta
    27241 acaacgaaag cataaaactt aatagaattc ctttaagttt agtcccgtga ggctaagaag
    27301 ggtgggagca aggatacgta tggtgcgcca taagtagatt gctcattgac atagtatagc
    27361 tatcagtgta tcagtatacg ctcagtctaa acattcctcc tgtcctctta gggcaggagt
    27421 ttttttatgc aaaaaagtag ggggaatgaa aatgcaagca aaagaagtag tagatcaggt
    27481 aaccatgaag cgagcattga ctcgaattac ttatgagatt atcgagagaa accatagtat
    27541 ccaagatatc gtattggtag gtatcaaaac acgaggaatc tacattgcat caagaattgc
    27601 cgaacgcctg aagcagctag aagatatcga tattcctgta ggagaactgg atatcacttt
    27661 ataccgagat gacaaaaaag aaaatccaga agaaccggaa cttcattctt ctgacatccc
    27721 agtttcctta gaaggaaaag aagttatttt gattgatgat gttttgtata caggacgaac
    27781 gattcgagca gcaatggacg cagtaatgga ttttggcaga ccaagaaaaa tttcattagc
    27841 agtcctggtc gatcgtgggc atcgagaatt gccaatccgt gcagattatg ttgggaagaa
    27901 cattccaaca tctcgggcgg aagagatttt agtagagatg caagagcttg atggacaaga
    27961 tcgcatcatg attttgaaag aggaagatta gaggagagag aaacatgtcg gagaaaaaag
    28021 gatttcataa tgatgacgtg gtgttagata tccacgatcg cccaacccca ggacattggg
    28081 taggattaag tttacaacac ttgttcacga tgttcggagc aaccgtgtta gtccccatct
    28141 tagtaggtat cgatccaggt attgcgctag tcagttcagg actaggtaca ttggtgtact
    28201 tatttactac taaaggaaag atacctgcgt acttaggaag cagcttcgct ttcattgcag
    28261 cgatgcagct tttgatgaaa acagatggtt atccagcgat tgcacaagga gcagtcacaa
    28321 ctggattagt ctatttgatt gttgcgctga tcatcaaaaa gatcggttca gcatggctgg
    28381 ataaaatctt gcctccaatc gtcgtcggtc cagtcatcat ggttatcggg ttgggacttg
    28441 cggcaaatgc agcaacaaat gctatgtata atcagggcac gtatgatttc aagtatattg
    28501 ctgtagcgct gatcacatta ggattaacaa ttttcttcaa catgtggcta agaggttttc
    28561 tcggattgat tccgatttta ctaggtatta ttagcggata catcgtagcc ttgctgtttg
    28621 gaatcgtgga tactaagcca atcgtagacg ctgcctggtt tgctgttcct aactttgaga
    28681 ttccctttgt acaatatgaa ccaaagctat atcttggagc aatcacaaca atggcaccca
    28741 tcgcttttgt gacgatgaca gagcatatcg gccatcttat ggtattgaat aaactaacta
    28801 aacgtaattt ctttgaagaa ccgggtcttc ataagacgtt gacaggtgat ggattagcac
    28861 aaatcgtggc agcctttgtc ggaggtcctc ctgtaacaag ttacggtgaa aacattggtg
    28921 ttctggctat cacacgtgtc catagcgtgt ttgtgatcgg tggagctgca gtatttgcag
    28981 tcattttagg attcgtagga aaattaagcg ctttgatttt aagtatccct ggaccagtca
    29041 tctcaggaat cagttttatc ttatttggag taattgccgc aagtggaatg aagattttaa
    29101 tcgagaacaa aatcgatttt gataaaaaga aaaatctgtt gattgcttca gttatcttag
    29161 tagtaggaat tggcggactc gtctttgaag taggcacatt tacattgtcg gcaatggcat
    29221 tagcaactgt acttggcatc attttaaatc ttatcttacc agaaacttca cgaagcgaag
    29281 aaaactaagg aggaaacatt atgatcatca aatcagaacg tatcagctta aagcatttat
    29341 tgaccgttga ggcacttact gatcaagaag ttatgggctt gatccgcaga gggcaggaat
    29401 tcaagcaagg tgctaattgg actccgcaaa agcgccagta ctttgctaca aacctctttt
    29461 tcgaaaatag tactcgcaca cataaaagct tcgatgtagc ggagaaaaaa ctagggctag
    29521 aagtgattga atttgaagca agcacaagct ctgtccaaaa aggggaaaca ctatacgata
    29581 cagtactgac gatgtcagca ttaggcgtag acgtagcggt tatccgtcat ggtgatgaga
    29641 actattatga cgaactgata caaagtaaaa caatccagtg ttcgattatc aatggaggag
    29701 acggaagcgg acagcatcca acccaatgct tgcttgattt gatgaccatt tatgaagaat
    29761 ttggtcattt tgaaggtctg aaagttgcca tcgtaggtga tatcactcac tctcgcgtag
    29821 ctaaatccaa tatgcagatg ttaaaacgtc taggcgcaca agtattcttc tcaggtccta
    29881 gagaatggta cgacgatgaa ttcgaagtgt acggacatta tatgccattg gatgaactac
    29941 tagatcaagt tgatgtcatg atgatgcttc gggtgcaaca cgaacgccat gatggaaaag
    30001 aaagtttctc aaaagaaggc taccatcagg aacatgggtt aacagttgag cgtgcgaaga
    30061 agttgcaaaa acatgcaatc attatgcacc cagcaccagt taaccgtgat gtagaattag
    30121 ctgattcatt agtggaagga ttacagtcgc ggatcgttca gcaaatgagc aatggagtat
    30181 tcgtcagaat ggcaatctta gaagcagtgc taaatggaaa agcgtaatta taggaggatg
    30241 aaaaaatgaa aacactcatt aaaaacggtc agatcaatac acgaaaaaat atgacgacac
    30301 cagcagaaat ttggatagaa gatgggcgaa tcaaagcaat cggtacagga ttctcagaag
    30361 ccgaatttga tgaagtgttt gatgcaaagg gacaattgat cacacctggt ttagtggacg
    30421 tccatgttca tttgagagag ccaggattta catacaagga gacgatcgaa gctgggactc
    30481 gctcagctgc tcgtggcggt ttcactactg tatgcgcgat gccgaattta aatcctgtac
    30541 ctgatacggc agaaaagctg agacaagtat atgacatcat tagaaaagat gcagttgtga
    30601 aggtattgca gtatgcgcca atcacagaaa atctgcgaag tgagaaatta gttgatcaag
    30661 aagcactgat tgaagaaggt gcttttgctt tcacaaatga cggtgtaggt gtccaaacag
    30721 ctgggacgat gtatctggct atgaaagaag ctgcaaaaaa caataaagcc ctagtagccc
    30781 atacagaaga tgaatcttta ttatttggcg gcgttatgca cgctggcaag aaggcagaag
    30841 aacttggtct tcccgggatc ttaagcgtaa ccgagtcttc tcaaattgct agagatctgt
    30901 tgctagctga agcaactggt gttcattacc atgtttgcca tgtttctact aaagaaagtg
    30961 tccgtgtaat cagagacgca aaaaaagcag gaatccacgt aacagcagaa gtttcacccc
    31021 atcatcttat tttgatcgac gaagatattc ccgaagattt cggcttttgg aaaatgaacc
    31081 cgccgctaag aggacgggag gacagagaag ctttgatcga aggcttgctt gacggtacga
    31141 tcgattgcat cgctacagac catgcccccc atggattaga agaaaaaagc caaagtttca
    31201 tgaaatcacc atttggtatc gtaggaagcg aaacagcgtt tcaactgatc tatactcatt
    31261 ttgtagaaac tggtcgattc acgttggaac aagtgatcaa ttggttggca gttaaaccgg
    31321 ctgagatttt tggtttgaat gcaggaacgc taacggttgg tgcccccgct gatgtagcag
    31381 tttttgatat tacacaaact tgcacgatcg ataaagaaga ctttttatca aaaggggaga
    31441 atacaccttt tatcggctgg aaagtaaaag gcgaaacaca aatgactttt gtcaacggaa
    31501 aacttgtgtg gcagaaggga gaataaaagt atggaacgtt tgcttatatt agaagatgga
    31561 acagttttcg aaggaaaggc ttttggtgca gatgctagtg tcttcggaga aatcgttttt
    31621 acaacaagta tgactggtta tcaagaaact atcactgatc aaagttttaa cggtcaaatc
    31681 attacattta cctatcctat ggtgggtaat tatggggtaa atagagatga ttatgaatcg
    31741 atttctccaa catgcaaagg agtagtagta aaagaacatg cacgtgtagc ttcaaactgg
    31801 cgaaaccaaa tgacgctaga ttcatttttg aaacaaaaag gaattcctgg aatatcaggt
    31861 atcgatacgc gcgcccttac tcgcaaaatc cgtgaagtcg gaacgatgaa agctagtata
    31921 gttgatgctg gagattcctt cgaacatgca tttgatcagc tgaaagcaac cgttctagca
    31981 accaatcaag tgcagcaagt ctcaactaac aaaccttatc caagtccagg aacaggtcgg
    32041 aaagtcattg tcattgactt cggcttgaaa catagtattt tacgagaatt gagtaaaaga
    32101 aactgtaacc tcacagtact tccctataac acagatgccg agacgatttt agcgctatca
    32161 ccagacggag tcatgctgac gaatggacct ggtgatccaa aagatgtgcc agaagcaatc
    32221 gaaatgatca aaaaaatcca aggaaaagta ccgatttttg gtatttgtct cggtcaccag
    32281 ttatttgctt tagccaacgg tgcagatacc tataaaatga aattcggaca tcgaggatta
    32341 aatcacccgg tacgagaagt agcgaccgga agaattgatt ttacttctca aaatcatggc
    32401 tatgcagtcg atgaagccac gattgatcca gaaaaactca tcgtaactca tgtagaagta
    32461 aatgacggaa cagtagaagg agtcaaacac agagattatc ccgctttcag tgtgcagttt
    32521 catccagatg ctgcccctgg tccgcatgat gcactacatt tatttgatga atttatggaa
    32581 atgatggacg caggaaagga gcagtaaaca gatgccgaaa cgtacagata tcaagaaaat
    32641 tatggtgatt ggatctggtc cgatcatcat tggtcaagcc gcagaatttg actacgcagg
    32701 aacacaagcc tgccttgcct taaaagaaga aggatatgaa gtggttttag taaattctaa
    32761 tcctgcgacg atcatgacgg ataaagaagt ggcagatcaa gtatatatcg aaccgatcac
    32821 acttgaattt gtatcaagga ttcttcgaaa agagcgtcca gatgctctcc ttccaacttt
    32881 aggcggacag acaggcctca atatggcaat ggagctggca gcatcaggta ttttagaaga
    32941 attgaacata gaacttctag gaacaaagct ttctgcgatc gatcaagcag aagatcgtga
    33001 tctattcaaa cagttgatga aagaattgaa tcagccaatc cccgagtctg aaatcgtcaa
    33061 tacagtagaa gaagctgtag attttgcaaa taaaatcggc tatcctgtca tcgttcgtcc
    33121 agcctttact ttaggcggaa ctggtggagg tatgtgtgaa aatgaagaag aattacgtat
    33181 cgtatcagaa aatggattaa gtctttcccc cgctacccaa tgtttgattg aacgaagtat
    33241 tgcaggtttt aaagaaattg aatatgaagt gatgagagat tcggctgata atgcaatcgt
    33301 tgtctgcaat atggaaaatt ttgacccggt gggtatccat acaggtgatt ctatcgtatt
    33361 tgcccctagt caaacattgt ctgaccatga atatcaaatg cttcgtgatg cttctctttc
    33421 gattattcga gcacttaaga tcgagggagg ctgtaatgtc caactcgctc ttgatccaca
    33481 tagttttaat tattatgtga ttgaagtcaa tccgcgtgtt tctcgttcat ctgcgttagc
    33541 aagtaaggcg actggctatc cgatcgctaa gctagctgct aaaatagcgc ttggattaac
    33601 attagacgaa atgaaaaatc ctgttacagg aacgactttt gcagaatttg aacctgcatt
    33661 agattatgtt gtagctaaaa ttcctcgctg gccgtttgat aagtttgaaa acggagaacg
    33721 gcttttaggc acgcagatga aagcaactgg tgaagtgatg gctatcggtc ggaatatcga
    33781 agagtcttta ttgaaagctg tgcgttcttt agaaatcggt acacaccata tcgaacttcc
    33841 tgagttggca gacatcacgg acgatgacct aatccaaaaa gtggttcgag cacaagatga
    33901 tcgcctgttt tatgtagctg aagctattag aagagggtac accatagaag aattagcgga
    33961 tttgacaaaa atcgatttat tctttttaga taaactgttg catatcgtag aaatcgaaca
    34021 gcagttgaaa cagcagccgt tggatgacaa gatattgaaa acagcgaaac aaaacggttt
    34081 tacagacaaa aaagtggcgg agctgtggga aataaacgaa gaagccgttc gccagatgag
    34141 aaaagaaaat gatttgattc ctgtatataa gatggttgac acttgtgcag cggaattcga
    34201 atcaaaaaca ccttattttt atagttccta tgaatatgaa aatgaaagtt cacgatcaga
    34261 aaagccctca attctagtac tgggatctgg tcctatccgt atcggacaag gggttgaatt
    34321 tgactatgcg actgtgcatt ctgtcaaagc aatccagcag gcaggatacg aagcaatcat
    34381 catgaacagc aatcctgaaa cagtttcgac tgacttttct atttcggata aattatattt
    34441 tgagccattg accttcgaag atgtgatgaa tgtgatcgag ctagaacagc cgataggagt
    34501 aatcgttcaa tttggtggtc agacagcaat caacttagcc gaacctttga ctgctgctgg
    34561 tgtaaaaatc ttagggacaa cgatcgaaga cttggatcgt gcggaaaatc gtgatttatt
    34621 cgagcaagcg ctgaaagagt tggatattcc tcaaccttta ggggatactg ctacaagtaa
    34681 agaagaagcc gtggttatcg cttcgagaat tggttatcca gttcttgttc gtccaagcta
    34741 tgtattaggc ggacgtgcga tggaaatcgt tgaaaaccag cgagatttag aagattacat
    34801 ggaacatgct gtgaaagcat ctccagaaca tccagtacta gtggaccgtt acttgcttgg
    34861 tggtgaatgc gaagtggatg cgatttgtga cggtgaaaca gtactcatcc cagggatcat
    34921 ggagcatatt gagcgtgctg gtgtccattc aggggattca atggctgtgt atccacctca
    34981 aacattatct gaatcgatca agaaaacgat cgaagattac acgatccgct tagcattagg
    35041 tttgaattgt gtcgggatga tgaatatcca gtttgtcatc catgaaaatg aggtttatgt
    35101 gatcgaagtc aatcctcgag caagtcggac tgttccattc ttaagtaagg tgacaggaat
    35161 tccaatggca caaattgcga cgaaagctat tttgggagag aaactaaaga cacttggtta
    35221 tgaaaacgga ctttatccag aaacaaaaga agttcatgtt aaagcacctg tcttttcttt
    35281 tacgaaattg cagaaagttg atacttactt agggcctgaa atgaaatcga ctggagaagt
    35341 aatgggatct gacagacaat tagacaaagc tttgtacaag gcattcgaag cttctggatt
    35401 acacttgcct gaatatggag ccgtattatt tacgattgcg gacgaaacaa aagaagaagc
    35461 actagaatta gctaaacgtt ttacagaaat tggctatagt ctgataggaa cgaaacatac
    35521 agctgcttat ttcgaaaaaa atggtctggt cgttgaaaca gtagcgaaaa tttctgagga
    35581 agcagcagaa aaaaatgtag tcgacttaat aagagatggt aaaacacaag tggttgtcaa
    35641 tacgattgat aaagatcggg gaaatgcttc taaagacggt ttcatcatcc gaagagaagc
    35701 agttgaacat ggtattccat tgtttacttc tttagacaca gcagatgcga tcgttcgagt
    35761 gatggaatcc cgtgcttttt caactaaccc tatttaacgg cgagcacatg ttaagacaaa
    35821 aagacgaaaa accgttgcag gaggggacag atgaaacaag aaatgatgga aatcgttcag
    35881 caaaaacagc tggcaccaaa gatttttgaa atgacattga aaggtgatct agtaaaagaa
    35941 atgcagcaac caggacagtt cttgcatatt ctcgtgccgc gtgcagactt gctattacgc
    36001 cgaccgatta gcttgaatca tatcgaccac gaaacaaata cttgccgtat catttatcgg
    36061 gtggaaggtg aggggactag agtcttctct actctttctg ccggagacca tttagatgtc
    36121 atgggtcctt taggcaatgg ttttgatttg tccaatcttc atacaggaga cgaagtattt
    36181 ataatcggtg gaggaatcgg tgtccctcct ctatatgagt tatcaaaaca gttagtcgaa
    36241 aaaggaatta agccaacgca ttttttaggt tttgccacac atgaagtcgt ttattatgaa
    36301 aaagaattta atcgtctagg agaaacaaga atcgctactg acgatggaac atacggtatg
    36361 cacggaaatg tcggtaattt attgttagga acgaagacgg aacctacagc tgtttttgca
    36421 tgtggttcaa acgggttatt gaagacagtg gaacagctgt tttcttcgca tcaaaatgtc
    36481 cagttatcat tagaatcccg catggcatgt gggatagggg cttgctatgc ttgcgtttgt
    36541 catacgccgg aagatgaaac aggaacaaaa agtgtgaaag tttgtgacga aggaccgatt
    36601 tttaaaatag gagaggtaat tatatgaatg agaatccttt agcggtcaaa cttcctggcc
    36661 tagaattaaa aaatcctatc atgccggcaa gcggctgttt cggttttggc aaagaatacg
    36721 gaaaatacta tgatttgaat cagcttggat cgatcatggt caaggcaacg actccaaatg
    36781 cccgctttgg caatcccacg ccaagagtgg cggaaacacc atctggcatg ttgaatgcaa
    36841 ttggtttgca aaatcctggt ctagacgttg ttatgcataa acttcttcca gaattagagg
    36901 agtacaatga gttacccatc atcgcaaatg ttgctggtgc ttgtgaagaa gactatgtag
    36961 aagtttgtag caaaattggc gaagcaccaa atgtgaaagc aatcgagctg aacatttctt
    37021 gcccaaatgt caaacatggc ggaattgctt ttggaacaga ttctgatgtt gcttttcaat
    37081 taacacaagc agtaaagaaa gtttcttctg ttcctgtcta tgtcaaattg tccccaaatg
    37141 taacagacat cgttccaatc gctcaggcaa tcgaagctgg aggggcagat gggtttacga
    37201 tgatcaatac tcttcttggt atgcggattg accttaagac aagaaaaccg attttagcca
    37261 atcaaacagg tggactctcc ggaccagcaa tcaagcctgt cgctattcgt ttgattcacc
    37321 aagtagcaag tatatccagc cttccaatca ttgggatggg cggcgtgcag acagtggatg
    37381 atgttcttga gatgtttatg gctggagcaa gtgctgtagc tatagggacc gcgaatttca
    37441 ccgatccgta tatctgtcct aaactaatta aagagctccc tgtgcgaatg agcgaactag
    37501 gcatcgaatc gctggaaaga ttaatcaaag aagtgagaga ggaacgtgaa cgatgaatca
    37561 actagaccaa cgaccaatta ttgcattaga tttttcaact aggcaagagg tagaagactt
    37621 tcttcgattt ttcccgaaag aagaaaaatt gtttgtgaaa atcggaatgg aattgttcta
    37681 ccaagaagga cctgaaatcg tacgttatct aaaagaggct ggtcataatg tatttttaga
    37741 tctgaaactt cacgatattc cgaatacggt cgaaaaagca atgcggggat tggcaaagct
    37801 tggtgttgat ctcacctgtg ttcatgccgc tggcggtatt cgtatgatgg aggcggcaat
    37861 gcgaggatta gaagagggga ctccagaagg tggaaaacgt ccgctacttc tagctatcac
    37921 tcagcttact tctacaagtg aagaagaaat gcatgccgat caactaatcg aagtttcttt
    37981 agaaaaaagt gtgatccatt atgctagctg tgccaaaaaa gcaggattag atggtgtggt
    38041 ttcttctgct tgggaagtag aagcgatcaa agaaatggca ggagacgaat ttgtttgctt
    38101 aactccgggt ataagaccag aaggaacagt cgcaggagat cagacaagag tagtcacacc
    38161 ttctcaggca aaaaagatcg gctcaacttt tatcgttata ggtcgaccaa tcactcagtc
    38221 aactaatcct tataaagcat atcaaacgat tcaaacagaa tggagctaac ctaaaatgaa
    38281 tgttgaacaa agtatcgcaa aagacttatt agaaattgaa gcagtatttt taaatccaag
    38341 taatcctttt acatgggcaa gtggaatcaa aagcccaatt tactgtgata accgaatcac
    38401 gatgagctat ccaaaagtcc gaaaagagat cgcaaaagga ttagcaagca aaatcaaaga
    38461 agcttatcct gaagtacaag tgattgctgg aactgcaaca gcaggaatcc ctcatgccgc
    38521 atgggtagcg gaaatactgg atttgccaat ggtctatatt cgaagtaagg cgaaagatca
    38581 tgggaaaggt aaccaaatcg aaggacgtat atttgaaggt cagaagatgg ttgtgatcga
    38641 ggatttgatt tctacaggcg gaagtgtctt ggaagcagct gaagcagcaa aaagagaagg
    38701 tgcggatatc cttggtgttg cggctatctt tacgtatgag ttgccaaaag gaaaagcaaa
    38761 tttcgaaaaa gccgaaatcc ctcttatgac gttgaccaat tattctgtat tgattgaagc
    38821 agctttagaa gatcgttata tcgatgagca agaattaact ctgctaaaag aatggaaaaa
    38881 agatcctgaa aattggcaga ttgaagcgcg cttgatgaag atagagaaat ccgatttgtg
    38941 tctccagaat catcaattgg aatagcaaaa atccaaaaac tgattgcgag aagcatcttt
    39001 tcatcaggta tgaaaagaaa cttctatcgt gatcagtttt tgattttttt aaaattcatt
    39061 tttctaagac tggttagcag aaagaagaga ataatctctt ctcttttttc tattatatat
    39121 agattttctc tattttttgg taagggagct gtttcaaaaa tggtaaagag cggaaatgtt
    39181 gcgaatatca gttatttaat gatatgatat tcggaaaaag aaatctcaaa gggaggtaac
    39241 ttttatgggg ttgaatgaat ttattttagg attgaacaat ttagagacga tcacaagggc
    39301 acctggattt tttaaatata cagagcatac agttgcagca cattcgtatc gtgtagcatc
    39361 tattgcgcaa gttctaggag acattgaaga agcgaatgga caaccgatcg attggaaact
    39421 gctctatgaa aaatcattga atcatgacta cacagagcgt tttattggtg atatcaaaac
    39481 gccggtgaaa tacgcaagca aagagttaag agggatgctg caaacagttg aagaaaagat
    39541 gacggacgaa ttcatcaacc aagagattcc agctgagttc caagaagttt atcgccgtcg
    39601 gttgttcgaa ggaaaagatg aaaccgtaga aggagaacta ttagccattt cagataaggt
    39661 tgaccttctt tatgaatctt ttgatgaaat catcaaaaat aacccggaaa aagtttataa
    39721 ggagatgttc attgaggcag tgactacgat taaaagcttt gaacatcggc cttctgttca
    39781 gtatttcttt aaagagatat ttccggaact attgaaccag gaattttatg gaaaagaggc
    39841 gtttttagaa accgtctcaa cgattgtaaa gaaagaagaa aaataaaaat gatatgagat
    39901 gcagggggaa gtatagttga aaaagaatat ggatgtcgaa tggggctatt ctggagacaa
    39961 aggtccggaa aattgggcga gtttatgtga ttggtttgcg agaggtgcgg aatttccatt
    40021 gcagtcgcct attcatttga ccaaggggga agaaaccaaa atagaaggag acacgctttc
    40081 ctttcattat caaaagcaac ggtttactga caaggaattc aaaaacacga ttcatttagt
    40141 accttttgac caactcagtt atgtagagtt caagaaagaa cgctattatt tgacagatat
    40201 tcatttccac ttgccttctg aacatatcat caacggaaaa caggaagata ttgaatttca
    40261 ttttgttcat atgaacagca aaaaagagaa tctagttgtg ggtgtcatgt accagctaat
    40321 ggtacaggaa ggttggctgt ataatcaaga aaatggggaa agttggaact tagaaaaaca
    40381 tgagcattgg gtaaatccgg atgtgttctt tccggagcaa aaaagccatt tccattatat
    40441 tggctcattg acaactccgc ctaccagcgg gccaatccaa tggtttgtaa tggatcatgt
    40501 aggaaaactg aaccaagagt ttcttctccc cttcgatggt cagtatgctc gaccgaacaa
    40561 ccgaccaatc cagcccttga atggaagaga aatttattat ttcgaggagt ttgagcaatg
    40621 aatattcagc agatgaaata cgtggtcgct gtagcaaaca atggtagttt cagagaagcg
    40681 gcaaagaaaa tgtttgtctc ccagccaagt ctttcagctg gtatcaaaga attagaaacc
    40741 gagttaggga tcacgctgtt tactcgaacg aatcgagggg catatttaac agaagaaggg
    40801 caagacttct taaatcgtgc cgagaaaata ttggtccagc ttgatacatt agaaactcac
    40861 tatctaacga gtgaaaagag agacaatttt tcgatcgctg cccagcatta tgatttctta
    40921 ggtcctttga ccgcacagct actcgaggaa ttcaaagacg agatcaaaca atttcggatc
    40981 atggagacga caacagcgag agtgatcgaa gaagtcaaag agcagcacag tgagatcggc
    41041 attatttata tgaatgagca taatcaagct ggtatcagaa gatatttaca acaaggagag
    41101 ctaagtggcc gcagcattgg aaaattcaaa acgcatattt ttttaaggca aggacatcca
    41161 ttagctgaac acgcagagat caccaaagaa gaattgttat cttatcctca agttcgtttt
    41221 acacaggatg gaaataattt cccgtatttt tatgaagacc ttatcgagat ccctgatcaa
    41281 gaaacagtga tttatacgag tgatcgaggt actttgatga atatcgttct tgaaactgat
    41341 gcttacgctt caggatctgg tattgtgatt ggagggattc gcgagcactt gcggttaatc
    41401 cctttggcag aaggcgaact aaatgaattt tatatcattt attcggcgaa acgtccgctc
    41461 agtgaaatcg ctcaaagatt tatcaaagag cttactcggt tactggacca gcagagctaa
    41521 gaaaagcaga tgagaacgct gtttattgaa caccgttgat caaaaaagag gggttgtgac
    41581 agaattttgt cacaactcct ctttttgtag caaacaattt ataactaatt gctgttgtgg
    41641 gaaagtacgc aaatatgatt ctagaaacac tcattgatta cctgttttgt ttcatagctg
    41701 taattttttc ttcttctggt gtcacgataa tcgtcttttg gttttcataa ataacataac
    41761 caggcttggc gccatttggt tttcggatat gctttacctg tacaagatca accggcactt
    41821 gggcagagta tcggtatttt gaaaaatagg cagctaactc ggcagcttct gtaattgttt
    41881 catctgaggg cttgtcactt ttaatgatga cgtgcgaacc gggtatattt tttgcatgaa
    41941 gccaatagtc ggttttttta gctgtcttca ttgtcagctg gtcattttga agattgtttt
    42001 taccaacaag aatgagcgtt ccgtcagaag aaaagtattg atcaggctga gatggtttct
    42061 tacgtttttg tttttttaag ttcttctttt tcaggtatcc ttctgaagtc aattcttctt
    42121 taattgcttc gatatccatt gggccggcaa tttccagttg agataagaca gattcaagat
    42181 attggatttc atctttggct tcttggattt gttcgccaat cagcttaaca gcgttcttca
    42241 gcttttggta tcgatggaaa tatttctgtg cattttgatt tggtgtcaat gccggatcta
    42301 gtgctatttt gattggccga tcttcttcat aataattagg taaaacaact tcttttgcgc
    42361 cgcgtggtac ttgtgtcaga aaagtagtaa gtagttcgcc atttctacgg taattctcag
    42421 cgttttcgga ttctttgagt gtttgttcac gttttttcaa tttattttta tttcgtttta
    42481 gttcattttc gatttttcga atcagttctc cgccttgttg ttttgcccga tctttctctg
    42541 ctttttcgtg ataatagcta tctaataatt gactgagtgt tggataagct gttacagcag
    42601 atgcctgttc ggacaatacc tgataagcaa tcggtgtgaa aaaatcttta ttttccgttt
    42661 cgtaaaaagt aggaataggc tgatggctga tggctgagaa aaattcgtcc catacaacca
    42721 tcttttcatt aggacgttcc agtaatcttt tggtcagctc ttgagcgata tcgaaaccga
    42781 ttccctgaaa ctgacgttgg attgcttttg gatctagttc agtttgggaa agccgctgaa
    42841 agattttttc tttctcggag ctaaatgggt tcaagacctc ttgttttggc ggagcaacat
    42901 attctacacc aggaagcaaa gaacgataag tgttttgaga gcttccaatg tgtttgatcg
    42961 catccagtat ttttccagtt tctcgattaa ctaagacgat cgtactatga cgtcccatta
    43021 attcaacgat caatatgata ttttgtagat cacccaactc gtttcgttta gcaaagtgga
    43081 aatggatcac tcgatcattt tcgatttgtt cgatgtcttc taaaatagcg ccatccaaat
    43141 attttcgaag catcatcacg aagttaggcg gattatcagg attctgataa tcgatttggg
    43201 taatctgtac acgagcataa cttgggtgcg cagataataa taatcgatgg tttttgccgc
    43261 gtgaacgaat caccagcacg atttcatttt cataaggctg atggattttc gagatccggc
    43321 cagatagtaa cgtttcacgc aattcgttga ccatcgcgtg ggtaaataca ccatcaaagg
    43381 acatggttca ttcctcattt cttttcataa gtatattcat tataacgaaa aaaagaagaa
    43441 aaaccaattt ggccgaatag taaagcaaaa gtctagaaaa atcttggact aattacataa
    43501 aaaacagtgt ttctatcgtt gaaatccaaa tttttaggta
//
LOCUS       DAPCMT010000010        42948 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPCMT010000010 DAPCMT010000000
VERSION     DAPCMT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.010"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(122..1096)
                     /locus_tag="RD743_000428"
     CDS             complement(122..1096)
                     /locus_tag="RD743_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDU0562286.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(1077..2960)
                     /locus_tag="RD743_000429"
     CDS             complement(1077..2960)
                     /locus_tag="RD743_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDU0562287.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(2978..5203)
                     /locus_tag="RD743_000430"
     CDS             complement(2978..5203)
                     /locus_tag="RD743_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDU0562288.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5318..6352
                     /locus_tag="RD743_000431"
     CDS             5318..6352
                     /locus_tag="RD743_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0562289.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(6419..7432)
                     /locus_tag="RD743_000432"
     CDS             complement(6419..7432)
                     /locus_tag="RD743_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0562290.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(7510..7698)
                     /locus_tag="RD743_000433"
     CDS             complement(7510..7698)
                     /locus_tag="RD743_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDU0562291.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(8138..8266)
                     /locus_tag="RD743_000434"
     CDS             complement(8138..8266)
                     /locus_tag="RD743_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDU0562292.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(8269..9642)
                     /gene="rlmD"
                     /locus_tag="RD743_000435"
     CDS             complement(8269..9642)
                     /gene="rlmD"
                     /locus_tag="RD743_000435"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDU0562293.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(9753..10808)
                     /locus_tag="RD743_000436"
     CDS             complement(9753..10808)
                     /locus_tag="RD743_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDU0562294.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(10818..12248)
                     /gene="gatB"
                     /locus_tag="RD743_000437"
     CDS             complement(10818..12248)
                     /gene="gatB"
                     /locus_tag="RD743_000437"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDU0562295.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(12245..13717)
                     /gene="gatA"
                     /locus_tag="RD743_000438"
     CDS             complement(12245..13717)
                     /gene="gatA"
                     /locus_tag="RD743_000438"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDU0562296.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(13717..14022)
                     /gene="gatC"
                     /locus_tag="RD743_000439"
     CDS             complement(13717..14022)
                     /gene="gatC"
                     /locus_tag="RD743_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDU0562297.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(14128..16164)
                     /gene="ligA"
                     /locus_tag="RD743_000440"
     CDS             complement(14128..16164)
                     /gene="ligA"
                     /locus_tag="RD743_000440"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDU0562298.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(16181..18415)
                     /gene="pcrA"
                     /locus_tag="RD743_000441"
     CDS             complement(16181..18415)
                     /gene="pcrA"
                     /locus_tag="RD743_000441"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDU0562299.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            18570..20129
                     /locus_tag="RD743_000442"
     CDS             18570..20129
                     /locus_tag="RD743_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDU0562300.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(20240..22171)
                     /locus_tag="RD743_000443"
     CDS             complement(20240..22171)
                     /locus_tag="RD743_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0562301.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(22189..23103)
                     /gene="pfkB"
                     /locus_tag="RD743_000444"
     CDS             complement(22189..23103)
                     /gene="pfkB"
                     /locus_tag="RD743_000444"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDU0562302.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(23100..23852)
                     /locus_tag="RD743_000445"
     CDS             complement(23100..23852)
                     /locus_tag="RD743_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDU0562303.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(24011..24220)
                     /locus_tag="RD743_000446"
     CDS             complement(24011..24220)
                     /locus_tag="RD743_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562304.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(24231..25559)
                     /locus_tag="RD743_000447"
     CDS             complement(24231..25559)
                     /locus_tag="RD743_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562305.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            25695..25850
                     /locus_tag="RD743_000448"
     CDS             25695..25850
                     /locus_tag="RD743_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562306.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(25924..26511)
                     /locus_tag="RD743_000449"
     CDS             complement(25924..26511)
                     /locus_tag="RD743_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDU0562307.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(26594..27844)
                     /gene="clpX"
                     /locus_tag="RD743_000450"
     CDS             complement(26594..27844)
                     /gene="clpX"
                     /locus_tag="RD743_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDU0562308.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(28091..29380)
                     /locus_tag="RD743_000451"
     CDS             complement(28091..29380)
                     /locus_tag="RD743_000451"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDU0562309.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(29534..30466)
                     /locus_tag="RD743_000452"
     CDS             complement(29534..30466)
                     /locus_tag="RD743_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562310.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(30623..31282)
                     /locus_tag="RD743_000453"
     CDS             complement(30623..31282)
                     /locus_tag="RD743_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDU0562311.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(31285..32310)
                     /gene="sppA"
                     /locus_tag="RD743_000454"
     CDS             complement(31285..32310)
                     /gene="sppA"
                     /locus_tag="RD743_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDU0562312.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(32323..32613)
                     /locus_tag="RD743_000455"
     CDS             complement(32323..32613)
                     /locus_tag="RD743_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562313.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            33022..34827
                     /gene="glmS"
                     /locus_tag="RD743_000456"
     CDS             33022..34827
                     /gene="glmS"
                     /locus_tag="RD743_000456"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDU0562314.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(34899..36254)
                     /locus_tag="RD743_000457"
     CDS             complement(34899..36254)
                     /locus_tag="RD743_000457"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDU0562315.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(36278..37447)
                     /locus_tag="RD743_000458"
     CDS             complement(36278..37447)
                     /locus_tag="RD743_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562316.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(37444..38331)
                     /locus_tag="RD743_000459"
     CDS             complement(37444..38331)
                     /locus_tag="RD743_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDU0562317.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(38520..42212)
                     /gene="nifJ"
                     /locus_tag="RD743_000460"
     CDS             complement(38520..42212)
                     /gene="nifJ"
                     /locus_tag="RD743_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDU0562318.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            42411..42932
                     /locus_tag="RD743_000461"
     CDS             42411..42932
                     /locus_tag="RD743_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDU0562319.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        11692 a         9276 c         7757 g        14223 t
ORIGIN      
        1 ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct tgttcatgac
       61 atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa tctaaagatt
      121 gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg taaggtgact
      181 caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca aaaatcgtaa
      241 gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc agttcttctg
      301 ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat cgcaacgaaa
      361 aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt tctacccatt
      421 tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg gggagtcctg
      481 ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt tcccctgata
      541 ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa cccaatgcgg
      601 gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt ttaccctctc
      661 cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt tccagtgtaa
      721 aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt actggagcaa
      781 tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact tttggataca
      841 atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg tcattatcgg
      901 tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt ccgtttttat
      961 tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc cccgatttga
     1021 cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata tgacttttat
     1081 ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc ttgtccatct
     1141 gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc aaagtcaccg
     1201 ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc ctgatcttta
     1261 aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt catgtaagaa
     1321 tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat gaacggtttt
     1381 tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat gcagcttttt
     1441 acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata atggacaagt
     1501 cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc ttccgcataa
     1561 gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt ttcgaaattc
     1621 gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg tacgttgtaa
     1681 gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt cgtttccgcc
     1741 atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt ggggtaatga
     1801 gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt ttccatatct
     1861 tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac cccgttgata
     1921 atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt acgaaaaccg
     1981 aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc caattcaagc
     2041 ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc atcgatgcga
     2101 atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc atcaggatct
     2161 agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa atctacttca
     2221 agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc ttctaaatgg
     2281 ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg gaaaaagtcc
     2341 tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag aatattttct
     2401 tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc ggcatacccg
     2461 ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa atgaatcgag
     2521 atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga accaaccgga
     2581 ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt cgtgaaaggg
     2641 ggacggcgaa atacatgtcc ctcccacgga tacatcgtat tgatgtaatg gatcttgtca
     2701 taatttgcta attcaatatg actaggaaca gtgatttcat caaaagaaca ggcatcaaaa
     2761 tcttttttat aaaatgtttt tggtcgtacc gaaggatttg gcgaataaca aaaacgccat
     2821 gatccattta aagactgttt aagggaggat gtggtttcta cttcttccct tgttgcataa
     2881 caaacatgat cgctatgtgc atttgattgg ttcactctaa aaacttgtgg atcatctaac
     2941 cattttatat ttgctttcat acttttttcc ttctctctca taactttttg aaatgaagca
     3001 gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag tccaactgtc atcaattcgg
     3061 cccccgaaaa tatccgcttc gaggtctgtt cctgataaag tgcctcatca tctaacccca
     3121 tcagatatac ttcatggaac gctggttgag ctgaagctag tatcctccct aaaaacacaa
     3181 tagcttcatc ttgtttagga gacacaaaca tccatgcggc aatattttct tcaaaaggac
     3241 tcgccaaacg ataataatgg ccatactgga tcaccggacg aatcgtttga taggatataa
     3301 tttggtcttt tacttgcttt ttttcttcag gagacagctt tgtcaaatcc agttcataac
     3361 caaaaacagc gctcatagct gtttctcctc ttgttttcaa cgaagtagat ctgtcggtct
     3421 gatgatttgg tacagctgat acatgagctg tgatggacga aatcggataa gcaaggcttg
     3481 ttccatattg gattttcata cgctccactg catccgtgtt atcacttgtc catgactgcg
     3541 gcatataata aataaacccg gcatcaaacc gtccacctcc accagaacag ccttcccata
     3601 agatttccgg ataatcagtt gtcaaatgtt caagtagttc ataaagcccg agcatataac
     3661 gatgcgctac ttctccttgt ctctgtgggt caagggtaat cgaatagaca tctgaaagcg
     3721 aacggttcat atcccatttg atgtagtcaa gaggaatcgt atctaaaatc tttctcattt
     3781 gtttctcaat cgtctgccga acatctaacc ttgtaaaatc cagtacatgt tgtgatctgg
     3841 aagcagaagg cattctacct ggctcctgca taagaaaatc aggatgtgtt cgatacagtt
     3901 cagaatcaac cgagatcatt tcaggttcaa accataaacc aaatttcaat cctttgctat
     3961 gagcataatc tgctatttca cgtaatccat ttgtcaactt gccttcatac tcgaaccagt
     4021 ctcctaatga agaattgtcc gcatctcttt ttccaaacca gccatcatct aatacgaaca
     4081 tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt gattttttca ctattgaagt
     4141 caaagtaagt ggcttcccag ttattgacca gaatcggccg ttcagcaaat tgatgacgac
     4201 ctcttgcgat tctttcacgt agcaactcat ggtggatctg actcattttg ttcaatccct
     4261 gacttgagta agaaaggatc atttcaggtg tttgaaaaga ctggcctgca ttcaattccc
     4321 acgaaaaacg atggctgttg atcccaccga cgactctcaa ttgattgatc tgatcttttt
     4381 caagttcgaa agaatggttg ccggaataaa caaattgaag tccgatcgct tctccagtat
     4441 tttctgtcgt atgatgatga actaaagcaa taaagttatt catatggtga ctgctcgttc
     4501 ctcgacggct ttcaaatact tttgttccag ataaaacaga ttgccgtgaa atctgtcttt
     4561 cattgacatg tgctcctggc aaagcaatga cttcatcaaa tcgcctagta tgggcaaagt
     4621 ctagctggaa actagccgct ttttctagtt tgattgtttc tccggtttca tttcttatct
     4681 ttacagatcg tgtgatcact gctctatcag taaatatggt atagaacaaa tcgaaataaa
     4741 tcgctgtttc tctatcctta agtgaaatca ccaacgtttg tgcctctgac ttgtccatca
     4801 cataagtttg aggcaagcct tcgatctctg gcttcccatc tgtaatataa tggctgtcat
     4861 atctcagatc cagtaagtct gatccattct tgcgctgaat gatcgatgcg ggcgttcgaa
     4921 agtccatgct gccatgagaa gagtattctt gcggtagtgt atctttcgac agagaccggt
     4981 caggatttaa gggaacattt cctgaaaatc cacgatctct tcggggataa tttttattgt
     5041 ctttgtactg cttgactggt ttgccaaaat aaatatgcgc caatacattg ctttcttcta
     5101 tctttatcag ataagaaaga aatgtatttc gtaaatggaa gactcggctc ttttcatcaa
     5161 ataagatgaa atttttatct gtttttctat gcgtaatatc catcatttca ctcccaacaa
     5221 aacggaaacc gtttactttt ttttataatt cctgtatact gtttactaaa caattttgtc
     5281 aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg gttggaatta gagatgtcgc
     5341 acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg ttgaataacg atcagacgat
     5401 ccatgtggct cagactactc ggcaacggat agtagaggca gctgctgcgt tggattatga
     5461 catcagtact cgtaaatata tcaaaaagag aatgccttcg attggtgtga tctctacgat
     5521 cggcaaagcg agcgaagaac aggatatcta ttacaaacat ttgcgagtag ggatggaaga
     5581 agaagctcga cgtctgcatt tagggatgaa tcgaatctat aatctatctg ataatcctaa
     5641 acaatgggaa gatctagatc agttaggtgc ggtgatcgtt gttgggactg ttacgaaaga
     5701 atcgattacc gccttgttaa aacaaaacat gcaaatagtc gtcgtggata accctgacat
     5761 ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg atgactaaaa ccgtactgga
     5821 attgttctta gaaaatggtc ataaaagaat agcctatatc gggggatata aagtcgatat
     5881 ggatgaattc ggtgggaagc agatttctat aaatgaaaag aggatgcgtg cctatcagca
     5941 ttttatgatc gatcatcact tagaaaaata tatgtcttat catctaggtg gctggacaga
     6001 aaaacaaggt gaggtacttg cgactaatct attagaaaat gaatcccctc ttcctactgc
     6061 cattcttgtc gggagcgatc caatggcaat tggtgtctat cgggcattga aacaaaaaaa
     6121 tatccaaatt ggagaagaga ttgttgtagc aagctttgat aatatcgaga caatcgcaaa
     6181 cctctcccct ggcttgacca gtgttcaaat caatgcgaaa tcaattgggc aagcagctgt
     6241 tcgaatggca ttggagcgaa ttgacggtat acgggacgaa ccaatcattt tgacttatcc
     6301 tgcacgattg attgttaggg aaagttttat cccaagaaca aaaaggtatt gaaagagagg
     6361 ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa cggctttttc acaacctctt
     6421 acttatactt attcacaaaa tccaatgtct gctggtaaca attttgcgat tcttttagcg
     6481 tgtaatcaaa ttggtattca tgggtaatct ctttttttgt atctgtataa aacaaggatt
     6541 gcacgggtac ctttaattct gtcaaccgat tttctaatgc ttgaccttgc tcctggaatg
     6601 agtatgcatt tccgtcagtt atatacgttg gcggaaattc cttagaaaca tggtcagcca
     6661 cactagcttc tttcaattgc tcactcgttt tccaatcttt tgttccaatc aacgaccacg
     6721 caacggtttt gacgaaaaac ttcataaatc gatcatccga atgctgctgt gctgttagct
     6781 tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag actatctttt ggaagtagct
     6841 gtttgatttt catttgttta gcatacgcat cattcgtttg tatagctgca tattgcaatg
     6901 cgatttgtga acctgcacta tctccaccaa aaaataattg ttccatgtct agcaatttgt
     6961 cgtctttttc ttcagctaat aaatctttga tcagttcatt cacttgaaga acttgattag
     7021 ggtattctga atcaggcgct aattcataat tcatcgcaac cactgcgatg ttggcgtctg
     7081 aaactaattt tgtcgcaaat tctttgacac ccgatttatc tccgccgacg aatcctccac
     7141 catgtaccca aaatagaaca ggaacaggcc ccttgctgtt ttttggatag tagatgtcat
     7201 aagtattctc tttatacttg gactcatacg tcaaatctgt ttttttcgcc acgttttttt
     7261 ctgcttgatc gaacgttttt ttatcagtga tttgaacagt atttgaaaac atatggttga
     7321 tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag aaagattgca aacgcgatcc
     7381 caagaatgat tccgaagaac catccgataa atttaagaat tttctttttc ataaggctcc
     7441 ctcagtagtt agtattaagc attctgatta taccatagtg ttatttccta tcaagctctc
     7501 ctagatgatt taatgatttt ccccaaactt cttctgcaat gcttttgaca aaagaaattt
     7561 tttccattgt tcttcaaaca aaacattttc ttcttctgtt gaagcaaatc cacattgagg
     7621 acttaagcac aaacgatcca aaggcagata tcgtgtcgct tcgtagatcc ggtcaatcac
     7681 ttgctggtgc tcttccattt cgccattttt cgttacagtc aaaatctaag acaactaatt
     7741 tatcttcaga gactttctct aaaagaacaa aactcccagc acgttcacta tcgtattcta
     7801 aataataggc acggacattt tcttgagtaa aataatgggt ctgctacgtc gtcatccccc
     7861 ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac atgtatattt agtgttgaat
     7921 catttgagaa tatgaaaata aaaatctttt tgatcaatca gttaatcaaa ttgaaataac
     7981 gaaaaatctg agacagaatg acgttcattc tgacccagat ttgattttgc acaactcaac
     8041 agtccttatt ggcaaatcgc caaaataatg tagactaact aaaaagacta agaaacaata
     8101 acaaaagtta acacttctta gtctttcttt agcacgtcta tttattaaag attttagggc
     8161 cttttctcat gtttttacga tcattcttag gtgtttccat ttgtttggat cggcctccac
     8221 cttgtttctt tttgatcaat tctttcattt tttctttttg gtccattttt attctccttt
     8281 aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg aaaagatcta caggctgaac
     8341 tttcttcacg gcataacctg cttctgcaaa taacttagca tctcttgcaa aagttgccgg
     8401 attacatgag atatagacga ttcgttctgg tgacatttcg attgctgatt caataaaatg
     8461 atgatctaat ccttttcgtg gagggtcaac tacgatcaca gatggtttga tccctttttc
     8521 ttgccatttt ttcataactt tttctgcttt tccgacttca taatgaacat tttcgatttt
     8581 attatttaaa gcattgaact gtgcatcttc aattgcttct ggaataactt ccatcccata
     8641 caccttatcc actctgtctg ctaaagacaa gccaatcgta cctattcctg aataagcgtc
     8701 gatcacaatg tcttttttat ctaactcggc aaactcgatg gctgtttggt acagatactc
     8761 tgcttgttga gtatttactt gataaaatga ctttgctgag attcgatagg tttttcctaa
     8821 tagctgatct tcgatatagt ctctgccata taaaactttt tctttgtctc ctaaaatcac
     8881 atttgttttt tcttcattga tattttggat gaccgaaacc acttctggca attcttcatg
     8941 aatagcttca gcaatttcct tggatttaaa gaatttttct ttacgagtaa ctaaaacgac
     9001 catcatttca tgggaataat gaccacgacg gataatgatg tgacgaagct gtccttcatt
     9061 tttttcttca ttatatcctc gtacttggaa ccgttgcaag atatctctga ctactttgat
     9121 tgcttgatcg atgacaggat cttggatata aaaatcttcg atagggacta gatcatggct
     9181 attctttttg taaaaaccgg tggctaactg tccatcgata cggcgaacag ggatctgtgc
     9241 tttattacgg tagccgaatg gttctgccat tccgaccgtt ggaagtacag gaacttctgg
     9301 catcttagca atcttcgcca ttacatgctc tacttgtttt tgtttgaaga caagctgttg
     9361 ttcatacttc aaatgggcta aaggtgcgat acctatgcga agtaatgcat tgttttctac
     9421 aggctgacga tcaggtgatt gtgtaataaa tgtttcgact tttccatatc caaacttatt
     9481 gcctgctttt actactagta tttctacttc ttctccaggg agtgcatttt cgataaataa
     9541 tggataacca tcgatttttg ctacccccat tccttcataa ctcaagtcga cgatttcgac
     9601 tgtatatcgt tcgtttttct ttactatttg ctgttctgtc atattttgct cctttaagcc
     9661 tatttttcaa tcctactgaa ctctactata aatcagctaa ttacacaact aaaaccggca
     9721 ctgaccttac tacgttcagc accggctttt gattaattct tttgattagg tgtttcgtct
     9781 gctatttttc cgtcaccgtc gatatcttca tccgttaaac gttcgacttc ttttacgaat
     9841 tctttgctga caacctctaa ttcttcttca tctgtacctg tgattgcttc atcaggaatc
     9901 tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat tttggaaatg catcggtgct
     9961 tccccgccgt attcaccatc taagttgatc attactggtc gattgatatc atcttctgtt
    10021 tcaacgaaca aactgcttgt tttggtatag attattcttg gatcatcgac atgtcgaccg
    10081 ccattcagca tgagtgctgc aatatgcaag atctcaaaga tatttgctgt cttcacaatg
    10141 attaaagaaa acttcccatc atccaatttg gcatctggcg cgatctgttc gaatcctcca
    10201 acagagttcg tcagccctag gaaaaacatc gaagcatttc cgatatattc cccttcatca
    10261 tacttcaagc gcattttgat tggctttact cgagggagca tctctgctcc tttcgcaaga
    10321 taagcaagat aaccaaaaat actctttagt tctgatggaa cttcataagt caattctgtc
    10381 aaatagccac ctgcagcgat attgatgaag taactgttat cagaaaggcc gatgtccatt
    10441 tttacggttt ggtgtttttt tatcacttct gctgctgctt taatattatc tctaggaatt
    10501 tttaatgctc gagcatagtc gttcgttgta ccagctggga tgatggccat tttaggacgg
    10561 tgttcaagcg gagcgattcc attaaccact tcgttgattg tgccatcgcc accagctgcc
    10621 actactagat caaaacctag ttctgcgact ctacatgctt cattcttggc tgaatctggt
    10681 tctggtgtag ttgcataggc gctggcttca aatcctgctt cttctaatgc ctgtaggata
    10741 tccgccaaat tttttttcaa taattctttt cccgaggtgg ggttataaat tacgcgtgct
    10801 tttttcattt tcagcctcta tcgtttcgct aattcatctt taagtagctt attcacgaca
    10861 ccaggatttg cttgtccttt agttgctttc atgatttgac cgactaggaa gccaaccgca
    10921 cgatcttttc catttttgaa atcatcgaca gattgttggt tgttatccaa aatttcatta
    10981 atgattggca gtagtttgtt cggatcagat aattgcaccc aaccattttt ttcaactact
    11041 tcttgggctt ctccgccatt tgtgatcaat tcacggaaga cttttttggc gatttttgag
    11101 ctgatcgttc catcggcgat caaacgaatc attcctgcta agttctcagg tgtcaatttt
    11161 gtctcagcta attccagttt ctcactgttc aagtaagcag aaacttcacc catcaaccag
    11221 ttagatgctt gcttggcatc tgcaccattt tccaaaacag cttcaaaaaa atcagacatt
    11281 tcttttgtaa gtgtcaatac cattgcgtca tactctggaa gtccaaactc acggatatag
    11341 cgttcgcgac gagaagctgg catctcaggc aaactagctt ttactttttg aatccattcg
    11401 tcatcgatca cgaatttcgg gatatcaggc tctgggaagt aacgataatc actagaccct
    11461 tctttgacac gcatcaaaac tgttttattc gttgtttcgt caaaacggcg tgtttcttgt
    11521 tggatttctc ctcctgaaag aagaactttt gcttgacgtt tttcttcaaa agctaaacct
    11581 tttttcacga aagataaaga gttcaagttt ttcaattctg ctttcgtacc gaactcttct
    11641 tgtccataag ggcgaagaga aatattggca tcacagcgca tagagccttc ttccattttc
    11701 acatcgctga cttctgtaaa ttggataatt gatcgcaatg cttctagata agcgtatgct
    11761 tcttctggtg aacgcatatc agcttcagaa acgatttcga tcaaaggcgt gccttgtcgg
    11821 ttcaaatcaa cgtaagaata accgccaata ccatgcatgt tttttccagc atcttcttct
    11881 aaatggacac gttcgatccg gatttttttc tttttacctt ctacttcgat ttcgatccat
    11941 ccgtcatgac cgattggttg atcaaattgg gaaatttgat atgcttttgg attatctggg
    12001 taaaagtaat ttttccggtc aaaatgagta tctttagaga tttcacagtt taatgccaaa
    12061 gctgctttca tcccgaattc cagcgcagct ttattcatta ctggcagcac gcctggataa
    12121 ccccagtcga tcacgtttgt attgctgttt ggttctgcac caaaatgagc tggcgctgga
    12181 gagaaaattt ttgagttggt ttttaactcg acgtggactt ctagtccaat gactgtttca
    12241 aaattcattc gtctttccct cctaaaatag ctggttgttg tttatggaat tctgttgctt
    12301 gttcaaacgc atatgccgct tgatacatcg ttttttcatc aaaataatta ccgatgatct
    12361 gcaatccaac tggtaatcct tctgataaac ctgccggtac agacatacct ggaagtccag
    12421 ccaagttgac agggatcgtt aagatgtcac tcatgtacat tgtgattgga tcgttaatgt
    12481 tctcaccgat tccgaatgca actgttggtg aagatggccc gatgatcaag tcataatctg
    12541 caaagacttt ttcaaaatct cgtttgatca atgtacggac ttgtcctgct tttttgaagt
    12601 aagcgtcata ataacccgcg cttaaggaga atgtacctaa catgatacgg cgttttactt
    12661 cttcaccgaa tccttctgaa cgtgagttga catatacatc atctaatgat ttcacatttt
    12721 cagagcgata accgtagcgg ataccatcga atcgttgcaa attggaactt gcttctgatg
    12781 aggcaatgat ataataaaca gccacaccgt attttgaatg aggcaagctg acttcttcca
    12841 caacagcacc taatgaacgg aatgtttctg ctgctttcaa tacagcttct tttacgccag
    12901 gggccacacc ttcgcctaag tattctttag gtaaagcaat cttcattcct ttgatgtcgc
    12961 ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga agaagtaccg tctttttcgt
    13021 cataaccgct gatagctgtc aatgctaatg cattatcttt caccgtacgg gtcattggtc
    13081 cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa acgtgagaca cggccataag
    13141 taggtttcat ccctacgatg ccgttaaaag ctgctggttg gcggatactt cccccagtat
    13201 cactacctaa agaaactgga atttgtccag ctgcaactgc tgctgcagaa ccaccagaag
    13261 agccaccagg tacttttgtt gggtcccaag cattttttgt tttcttgaaa taagaagttt
    13321 ctgtacttcc acccatagca aattcatcca tgtttagctt accaacaggg atcatatctg
    13381 cttgatagat tttatccatg accgttgcat cataaattgg ttcaaaattg taaaggatct
    13441 tagatgcagc ggttgtcaag atatcttttg ttacgatatt atccttgata ccaattggaa
    13501 taccagcaag gacgttgctt tcatcaatcc ctttttcatc taaagctttt gcctgttcca
    13561 atgctttttc ttcgttcaat gtaatgaatg cctcgacctc aggctctgtt tctttgattc
    13621 gttcaaatgt tgtttttgtc aaatcttgta cagtgatttc tttcgataca agcatatgat
    13681 gcagttcttc tagagaacga ttgtgtaatt ctgtcattat gcgccagcct ccccattatc
    13741 gatgattgcg ggtactttga taaagccgtc ttctgtttcc ggcacatttt tcaataattc
    13801 atcgcggttc catccagctt gcgcccgatc ttgtcgcatc acgtttactg tttcaaccac
    13861 attcgacgta aatggaacac cttccgtatc tacttcttcc agcatttcaa ccatatcgat
    13921 gattttacct aattggtcgg taaaacttgc aagttcttca tctgcgaacg caagttttgc
    13981 taatttggca acgtgtttca cttgttcttc actaatcgcc atactacccc ttctttctaa
    14041 acgagttaaa aactttgctc tctattttaa cacaagagtg ccgtgtctga aattgtttat
    14101 tgaaatttgt ctgaaaaaac tcatttttca ttcttctact gagcgacttt tagccaatgc
    14161 atctgccatt tgatcttctg tccaaacttc gattcctaat tcttgtgctt tggtcagctt
    14221 gctgccagca tcttctcctg ctaccacgat atccgttttt ttcgatacgc taccagtcac
    14281 tttcccgcca agattttcga tcgtctcttt tgcttcttca cgagtaaatc gtgttagttt
    14341 accagtaagg actactgttt tttctttaaa aggagactcg acttcttgca attgggcatt
    14401 tcgaagccct ttgtattcga aattgacgcc tgcttgtttt aattcattca tcaattcatg
    14461 gacttcttcg ttactgaaat aagtgactac actgtcggca atgatatcac cgatcgtatc
    14521 aagcgcaata atcgactcat agtcactttt actcaatgtt tctagatcac cgaaatgttc
    14581 tgctaaaatt tttgctgctt tcgctccgac atgtcggatt cctaacccga aaatcaaccg
    14641 ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa atattatttg ctgatttttc
    14701 ttttattttg tccaacgtca ataactcttc ttctgtcaat ttgtaaagat cagcaacatc
    14761 tgctactagt tttttatcat acatctgttc tagtacgcgc ggccccaacc catcgatatt
    14821 catcgcattt cttgaaacaa agtggttcaa accttctttc atttgagcag ggcatttagg
    14881 attgatgcac cgtaaggcta cttcttcatc caaatggaca agttcacttc cacaaactgg
    14941 acaatgtgta ggcaattggt attcttcgct gtctttaggt cgtttatcta acacgacttg
    15001 cgaaacttct gggatgatgt ctcccgcttt ataaatcaga actgtatctt tcaagcggat
    15061 atccttcaac ttaatataat caccgttatg caggctggca cggcttactg tcgttcctgc
    15121 tactcggact ggtgtcatga tagctgttgg cgttactacc cctgttcgac cgactgtcca
    15181 ttcgatgttt tcgattagcg tttcaacttc ttctggcgga aacttgtaag ctgctgccca
    15241 tctcggtgct ttgatagtaa aaccaagttg atcttgtagc gagaattcat tcactttgat
    15301 gacgattcca tcgatttcat agggcaaatc cacacgtttg tcatgatatt cttcaatgta
    15361 tgaccacact tcatcaatcg aatgacatag acgtttttcc cgattcgtgt ggaatccaat
    15421 tttttctagc tcttctagcg catcatactg ggtttttgct tgcataggac cgaaatcagc
    15481 tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg attttagaat ctaactgacg
    15541 caaagaacca gctgcggcgt tccgaggatt cgcaaagata tcttttccct ctgcttctct
    15601 gtcttggttc aattggacaa atgaccgttt tggcatgaaa cattcgcctc ttacttcgat
    15661 gttcattggt tcttttaatt tgattgggac cgaccggaca gtttttaggt tttcagtgat
    15721 gttttctcct acagttccat ccccgcgagt cgcacctctc acgaaatttc catcttcata
    15781 acgcaaagaa acggaaagtc catctatttt tagttcacag caatagtcaa ctacacgtcc
    15841 cactgctttt tgtacacgtt gatcaaaagc tatcagctct tctttgctga aaacgtcgtt
    15901 caaactgtaa agcggaatat catgagtgac tttttcaaaa ccttctaata cttttccgcc
    15961 tactcgttga gtgggagaat cagacgtgat caaatcagga tattgttcct cgattgctac
    16021 taattctgcg tattctttat cataaacata gtcttccact gttggtttat cttctacgta
    16081 gtattcgcga gaccattggt tcagtcttgt acgaagttct tctgctcgtt tagcagcttc
    16141 gtcaatcgtc atttcatgtt ccatcattta ccctccacta ctaagctttt tcaattggcg
    16201 caaaggcagc caaaagacgt tttactcctt gacttgggaa agccacatct agttctagat
    16261 ccttcgacgt cccgcttact cggacgactg ttcctagacc ccattttttg tgtttgactt
    16321 tatctccagg tttccagctg ttattttccc ctccagtagc acttttagag ggaacaaccg
    16381 ctgtctctgg ctgtttatag ctatatgctg tttttttagc gaatacaggc gtttttttag
    16441 gcgtaggacg catgccttcg atttccagta actcctgatc gatttcttct acaaaacgag
    16501 aaggtcggtt atattgcgtc cgtccatata atgttctgga gaaagcattt gttaaataca
    16561 acgcttcttc tgctcgcgtg atccccacat atgcgagacg gcgttcttcc tccagttcgc
    16621 tttcttccat caatgctcga gataaaggaa aaaccccctc ttctagacca atcaggaata
    16681 caactggaaa ttctaatcct ttggctgcat gcaaagtcat caatgtcact tgggaagcat
    16741 cttcttctaa attgtcaatg tcggaaacaa gtgccagatc atttaagaag accgttagtt
    16801 tttcttctgg tgcatcagcg tcttcttcat tttgctgttc gaattgtttg tcgaattctt
    16861 gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc ttgtgcttct aatgtatttt
    16921 ggatttttaa gtcttctaga tatccgctac gatccaaaac ctctttcgtc aattctgtca
    16981 ctgtcaagta tgggatcatc tgagtgactt cttggatcat ctctccaaat gcacctaatt
    17041 gctgtcctgc ttttccaccg atatttgcca gatccacatt ttgcgctgct tcaagcagtg
    17101 gccattcatg aagagaagca aaactgcgta gtttttcgat agatcctggt ccgatgcctc
    17161 gtttgggcga attaacgata cgctcaaaac tgattgaatc ttgaggattt accagcacat
    17221 tcaaatacgc taaaatatct ttgatttctt tacgatcgta gaacttgtgc cctccaacca
    17281 ttttataagg aatatttgct ttcaacagag tttcttccat tacacgagat tgtgcgttcg
    17341 tccggtacaa gatggcaaag tcgccgtaat tccgatgatt gcttcgaatc tcttcttgca
    17401 tccggtctac gataaaacgg gtttcgtctc gttcattatc tgcacggtag taagtgatct
    17461 tgtttccttc tttgttttct gtccacaagt tttttggttt tcgattgcta ttgttttcaa
    17521 tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg atagttttgt tctagcaaaa
    17581 ttactgcagc atctggataa tctttttcaa aatccaagat gttctgcata tctgcaccgc
    17641 gccagccgta aatactttga tctgcatctc ccacgacgca caggttccta aatctacctg
    17701 ccaataaatt gactagcgta tactgtgcgt gatttgtatc ttggtactca tctacatgaa
    17761 tataatggaa tttgttttga taatacgtga gtgaatcagg atgttcttca aataatcgaa
    17821 tcgtattcat gatcaggtcg tcaaagtcca tacattgatt atattgaagc tctttttggt
    17881 aagcagcata acattttgcc gcaatctctt cgtaataact gccttgcatt tttgcaaaat
    17941 cctgaggggt ctgcaggctg ttttttgctt gagagattgt tcctaatata cttcgaggat
    18001 catatttttt cggatcaatg ttcagttctt ttagtatccg tttcatcaag gtcagctgtt
    18061 cagaagaatc gatgattgta aagttccggt tatacccgat aaaatcaaca tctcttctta
    18121 aaatacgcac acacatcgag tgaaaagtcg atacccagac atcttcgcct ccagatgcga
    18181 gaatcgcatt gacacgttct ttcatttctt ttgcagcctt attggtaaaa gtaatcgcta
    18241 ggatgttcca aggattgact tctttttcct caatcaaata ggcaatgcgg tgtgtcagca
    18301 ctctcgtctt tccacttcct gcacctgcca ttagcagtaa tggtccgtca gtgtgaagaa
    18361 cagcttcttt ctgacggggg ttcatcccgt ttaataattc agctttggat gacattaaca
    18421 acatcctttc ttttatcaat cttgttaatt atagcatttt cttggctttc attaaactag
    18481 ctagttcaaa atcatttgtt tctttttatg gatttttcct tgtcaattcg ttgatttcgc
    18541 gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa aggaaaaatc aagaaattag
    18601 ataagactca gatatatttt attctaaaag gaatatttgt tggactgctg acaggaatcg
    18661 tcgttagcct gtttcgatta tctatcgaaa agctaagcga tttagttcga agtatctatg
    18721 aaatgagtcg ggaacagccg atttacttaa ttggcctagc tatttgctgc atccttgcag
    18781 catttttcgt gggttatctg gtcaaaaatg aaccggatat caagggaagc gggattccac
    18841 aagtagaggg acagcttcgc ggggaattat caatgaactg gttctctgtt ttatggaaaa
    18901 agttcattgg cggtgtactt tcagttggtg cgggcctttt tctaggtaga gaaggacctt
    18961 ctatacagct tggcgcatcc attggacaag gagctggaca attgtttcgg agtccatctt
    19021 cagaagaaaa gattttaatt tctagcggtg caagtagcgg acttgcagca gcattcaatg
    19081 ctcctatcgc aggattactc tttgttttag aagaaatcca tcactccttt tctccacttg
    19141 tttggctgac atcatttgct tcagcaatca ctgctaactt cgtctctctt tactttttcg
    19201 gcttgagacc tgttttatat ctaggtgacc ttccttcttt gccgttgaaa tattatggtt
    19261 ccttagtgat cttaggagta cttctaggca tcttaggttt tatttatcag aatgtcttgc
    19321 tggctttgcc aaaattttac aaccgattgc ctttaccagc ttattttcac gggttcgttc
    19381 ctttcttgct gattttgcca attggttatt tatggcctag tactctaggc ggaggcaatc
    19441 aaatcgtttt agcttttgga catgcttcct tgccaatcat tatgattatc ggtttgtttg
    19501 ttcttcgatt cgttttttcc atgatttctt acggtggggg tcttccaggg gggattttcc
    19561 ttccgatatt gacattgggg gctttaatcg gtagctttta tggaaacatt atggttgatt
    19621 tcgggatgga tccaatctac atcaaggatt ttgtcgttat tgccatggct ggttatttca
    19681 cagcgattgg aaaagcaccg ctgacagcaa ttattttagt gacagaaatg gttggctctc
    19741 ttaatcatct gatgccttta ggattggctg ctcttgttgc ttatatcgtc aatgaccttc
    19801 tcggcggtaa tccaatttac gaatcattat tggaacgtct acttggaaat actcagaccg
    19861 cctcactaaa aggtgcaagg acaacttttg attttccagt aacggcagag agttctttag
    19921 acggattaat ggtacgagac ttcaattggc ctaaaaatat gctgctaatc tctatacgca
    19981 gaggaaatca ggaaatcttg actcacggtg atacgatcat gaatgtagga gatattttgg
    20041 ttatcttgac tgatgaagca aacttgccaa gaatcaagca ggaaatcgag gctaaatctt
    20101 ctatcacaaa tttgaagcaa tcattttaac gaacatggaa aaactatttt acaacaacta
    20161 ttcatctaca ctacttgatt ctatatgatt ctataaaaaa agaagaggct gggaaaaccc
    20221 agcctcttct tttttatttc tatgcttctg ctactgcagg agcttttctc aataaaccat
    20281 aaatcacacc agatacgact gctccgatag caataaacac caaatacatg accgggtgac
    20341 tcagtaagaa tacaacgaaa atcccgccgt gaggagccag taatttgatt ccgaaagctc
    20401 cgactaatcc gccagttaaa gcagaaccaa taataaaact tggaatggca cgaatcgggt
    20461 cagctgctgc aaatggaatc gatccttccg tgacaaacga tagtcccatc acgatgttcg
    20521 tcaaaccagc atcttgatct gttttgctga atttattctt aaacaaacgt gtagcaacga
    20581 aaattgccaa tggaggtacc attcctccag ccataacaga agccatcacg acacttccgc
    20641 cagttgcaac agtcgtagct aaagttgctg taccaaacac ataagcggct ttgttgattg
    20701 gaccgcctaa atctgccgcc atcattccag ctaataatgc accaagtaaa gcagcattcg
    20761 aaccactcat acttgtcaag aaaccgttca atgcatcatt gattgctttc atagggacat
    20821 tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa cactggataa aagaggatcg
    20881 ttttaattcc atccaatgat tttggtaagt tcttgaataa tttacgtaaa aatacgatga
    20941 cataaccagc taagaatcca ccaatcagtg cacctaagaa tcctgcaccg cctgtattag
    21001 ctaatgcacc agcagcaaat ccaacgatca aaccagggcg gtcaccgata cttgaagcaa
    21061 taaatcctgc taataccggc agcatgaaac caaatgcagc ttgtccgatt tggttgaacc
    21121 agctagcagc ttgattgtaa ttccctagat ttgataattg atcatgtggc acgccaataa
    21181 attggtcgat catgaaagaa agcgcaatag cgattcctcc accaataacg aaaggtaaca
    21241 tgtgtgatac gccgttcatc aaatctttgt aaattctttg tccgatagta ccatccgcat
    21301 ttccttcttc tgaactgctt tcttttccat caccatggaa tactggtgca gtaccactaa
    21361 gggctagatt gatcaactct tctggtttac ggataccatc actgacagga cgattgatca
    21421 gttcttttcc gtcaaagcga ttcatctcta cttttttgtc tgctgcaacg attacacctt
    21481 ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat tccttctgaa ccgttcgttt
    21541 cgactttgat ctccacaccc atttctttcg cttttttctt caaagcatct tctgccatgt
    21601 aagtgtgagc gatccctgtt ggacaagctg taacggcaac aacgaatttt ctttctggat
    21661 gagcagaagc ttctgcagct tgtgtttctt gttctgcttt ttcttctgct tctttttgct
    21721 cttcagctgt ttggaacaag tcttggactt cttctggtgt atttgcttct tttaattttc
    21781 ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc taatgcttgc aaatgtgtgt
    21841 cgtttgcgcc ttctggagct gcgatcatga aaaacagata agttggctgt ccgtctaagg
    21901 cttcataatc tactcctttt tggctttttg cgaataaaac tgttgcttct ttaactgcac
    21961 tgtttttcgc gtgaggcatg gcgattccat caccaagacc tgtagaagtt tgtgcttcgc
    22021 gggctagaat tccttcttta tatgtttcga tatcggaaat ccggccggca tcaaacattt
    22081 tttggaccat ttcatcgatt gcgccttttt tatcagtcgc ttgcaaatcc atgatcatcg
    22141 cttctttgac cattaaatcc ttgatattca tgattatcct ccttgaaact aatcaatttt
    22201 tgtgatttca acttctggca gcaactcatc aatcaattcg cgagctgcca agtcatcaga
    22261 aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag gcttcgattg gatctttcgt
    22321 acggctgtaa gccccaataa agccagcaat cattgaatcc cctgcaccta ctgaattttt
    22381 caatgggcga actaatacat tcgttttata agttccttgt tccgtgaaga gcaaagcacc
    22441 gtctccagcc attgagatca gcacattctg tgccccttca gctagtaatt ttttcccata
    22501 cggaatgatt tcttctacag attggaaggt tgtgttgtaa agctcagcta attcgtgatt
    22561 gtttggtttg atcaacaacg gtttcttaac aagtgcattt tttaggtcat cacctgttgt
    22621 atcaatcacg aaatctgcac ctttttctcg aatgatatca atcagttctt gataaaatcc
    22681 ttttcgcagg ctagctggcg cgcttcctga caatacgacg atatctcctt gtcctactgt
    22741 tcctaatgct tgcttcaact catcgatttc ttcatctgaa atagatgggc ctaagccatt
    22801 gatctcagtt tcagtgtctg attttagttt gatattgatt cttgtatctt cttttacggt
    22861 tgtgaacaaa gactggattt gttctttttc aagccacttc gaaataaatt cgccagtgaa
    22921 ccctcctaaa aaccctagtg ctgtagattg agaatcgatc cgtttcaaaa tacgggaaac
    22981 attgatccct tttcctccag gtaacttgaa gtcttccttc attcggttca aatcgcctag
    23041 ttttaaccca tctacgcgta cgatataatc aattgatgga ttaagtgtta ctgtgtaaat
    23101 cattgtattg cctccttaat tgttgtcttt tcacggaatt cattaacgaa ttctaatgga
    23161 caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa cagaggtgaa ggacaactgg
    23221 ttgaatttgg tatgatcaat caatacaaat gtctcttcgg aatttttcat ggctaaacgt
    23281 ttaacagctg cctcttctgg atccggagtc gtgaatcctt gttcaagatg ggcaccgttc
    23341 attcccataa atgctttatt aaaatgaaaa ttttttagtt gttctatcgc attagatcct
    23401 aaaacagcat tagttgccag ctttatcgtt ccacctaaaa taatggtagc aagctccaaa
    23461 tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta ctgtgatctg ttttccttgc
    23521 agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta aataaatgac atcttcgtca
    23581 ttaatggttt cagctgccga tttggcgatt tgctgttttt cgtgaacgtt tttgagtgat
    23641 ttttcactca tattctgctc aaaattcaat aattgctctt tcttagctcc gccatgaact
    23701 ctttctagaa acccactatt ctccagctct tgtaaatcac gtcttatcgt tgattcagaa
    23761 gcattcaaaa ggtttacaag atcttgtgat ttcacgattt tctgctgttc aagcaagtct
    23821 aggatttttt gtctgcgttc ctctgtaagc attttcacga tcacctcaat acaaataata
    23881 gcatattttt tttctactag caatataaat cagtcaaaaa cgttcataaa aattataaat
    23941 cagtcaaaaa aaaataaaaa accagtcaga aagactggtt ttttattttt ttgtctatca
    24001 tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt tttcatataa acagaagcag
    24061 gttttctttc cttatgaaga agataataat aaagtcccat cccgccaaac atgaccaaaa
    24121 aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa gagacagacg gataataatg
    24181 atgccggaac cattattcga taatagagtt gtttatccat tttcttcacc tcatattttt
    24241 tgtaaagtaa tcaattgttc taattctcgg taatctaaaa attgcacacg cagatgctgc
    24301 tcacctttct tgacatcgaa ttggatactt cgccgctttt tctttattcg tttgaaggta
    24361 acatcagaga gtctatgttt gatttccttt aacggtaatt ctgacaggga ttctgaagga
    24421 acatagattc ttaaggataa aagatactct tgatattttt catctgttac ctctacttct
    24481 agatattccc taatataata acaataagct tcgattccat tatccaaagc aacagtcgga
    24541 tcgataactt tatagcacat catgcgtgat cgattctcgt caacccaagg aaaatacgcc
    24601 tgagcattta gtgttccgcg aacacgcctt tctttataat ctccttgttg gatatcataa
    24661 ctttttgtta caaaaaatga agcattcttt ttaggaacag gttgcatttg tgcattctga
    24721 tactggacag attggatcgt atggtcagtg taaatgtatt caacgaatgg cggtttcaat
    24781 aaaccgacac cattgtccca agcaagatga tcaaacccaa gctggtccct aaaataggat
    24841 tgaaacattt gattgtttgg cttttgccag ttaaaggggt ctcgataaag ctggatacat
    24901 atgacttcgt tttctaattc cttcactttt tctacttgtt ggatgtcgag aaaaatcggt
    24961 ttagggatga tttgatgata atagcgacta taatacattt cccaacacga agtgaaacat
    25021 aaaccttggt aaaaaagatc gtatcctgga aattggttgc aatcgactac tacttccccg
    25081 ttttgttgtt tcattttagg taaatgttcg atttcatcct ttttctcaaa caatagccgt
    25141 tgagagatct gttttgtatt gtgatagata aactcgctat aagctcgcat ataggcgaac
    25201 accccatgag caatcatttt tttctgaatg aagtctcgaa tcaaaaattg cttttctttg
    25261 aaaacagaga tagacaatac attttttatc aataattgat tatcttccaa gcgaaaacta
    25321 cattcgttct cgtcatttcc tacaactaac caactttcgt ctcttaccga tgctagcatt
    25381 attttttctt gaagctttcg aagatcatac ggatccgctt gttgtgtgaa agagcggata
    25441 aatgttggct ggatcaacag acgtgtcaaa atagcccaat cgcttatttc ctgttttttt
    25501 tcttcttcat ttaagtctgt gagctggata gtcatcaata atttatcttc gccaatcatt
    25561 ttctcacctc tttcaaatct ttttataatt taattataac caatgataaa gaaaaactaa
    25621 aactttatgt cttagaaacc aatttggacg attacatgat agactaagta aaaagaaaca
    25681 aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga taatttagat aaacttttcg
    25741 aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa agaagagatc gatcgatttc
    25801 taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa acaaaagtaa gttaagctca
    25861 aaaaaagact taccagccaa acttttatga aagagtttga ctggtaagtc ttttttattc
    25921 tttttatgat tcaattgctt gttcgattgc tgcccacgct tcttcttttc cctgttttgt
    25981 aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa tccaacgctt ttttgatttc
    26041 gctctcatgc ttgttccatt taccacgagg gatcttatct gccttagtgg caacaacaat
    26101 tacagggata tcataatact tcaaaaattg atacatctgg atatcttctt gagaaggctt
    26161 atgacgcaaa tctactaaag aaacgaccgc acgtagctgg tctctagtcg ttaaatatgt
    26221 ctcgatcatc tttccccatt ttgctcgctc agttttggat acttttgcat acccatatcc
    26281 cggaacatcg acaaaatgta atgcgttctc gatcaaataa aaattcaatg tctgggtctt
    26341 tcctggctta ccggaagtac gggctaaatt ctttcgatca atcaatgtgt tgataaaaga
    26401 agatttccct acatttgatc gtcctgccag agctatttct ggcaattgtg tatctggata
    26461 ttgttttggt gccactgcac ttatgataat ttcagcttga tggactttca tggtctctct
    26521 ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa agccaaaatg acttttacgg
    26581 cagcctcttt tcatcaaccc gcttttttct ctgaatcttt atagattact gttggctcac
    26641 cagacaattc agctgcttct tttgtgataa tcactttttc gatgttttca tttgaaggga
    26701 tatcaaacat cacatccatc atgatttctt caatgatgga acgaagtcct cgagcaccgg
    26761 tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc ttcaggttcg aattcaagtt
    26821 cggtattatc tagagaaaga agtttttgat attgtttgac tagcgcattt ttcggctcag
    26881 tcaagatccg taccagatca tctgttgtca atttctctaa agctgccata acaggtaatc
    26941 gtccgataaa ctcagggatc aatccgaatt ttagtaaatc ttcaggaatg atatgctgca
    27001 tgacactttc gtcttcagaa agtttttggt tttgtgtacc aaatccaatc gtcttttcgc
    27061 ccatacgatt cttcacaatc gtttcgatgc catcaaatgc accacctacg atgaacaata
    27121 tgttcgtcgt atcaatctga ataaattctt gatgtggatg tttacgtccg ccctgaggtg
    27181 gcacacttgc aacagttcct tctagaattt tcagcaaggc ttgttggaca ccttcgccgg
    27241 aaacgtcacg agtaatagat acattttcgc ttttacgcgc aattttatcg atctcatcaa
    27301 tatagatgat ccctttttct gcaagttcca cattgtaatc agcagattga agcaatttaa
    27361 gcaaaatgtt ttctacatct tcccccacat aaccagcttc tgtcaaactt gtcgcatctg
    27421 cgattgcaaa aggtacattc aatgtttttg ctaatgtttg tgctaaaaat gttttaccag
    27481 accctgtagg tccgatcaag cagatattgc ttttttgcag ttcaacatca tctgtgtttt
    27541 cttgtgcttg ctgattgaca cgtttatagt gattgtatac ggcaacagct aacgagcgtt
    27601 ttgcgcgatc ttgtccaatc acgtagttat taaatacttc aagaatttct agtggtttag
    27661 ggacttcagt gaattcgcgt acagcttctt cataaaattc ttcatcaatg atttctttac
    27721 ataggtcgat acattcatta cagatgtaga cccctggacc agctacgatc tttttcactt
    27781 cttcttgggt tttcccgcaa aaagaacagc gtacggtctc attgctactt ggattgtcat
    27841 acatggtctt caccccttaa cttgcaaaaa gtatgcaaga aactatcaaa gactttacag
    27901 tcttttacta tcataacata gtttagtaaa aagaaaaagt gtttaacaaa ggatcaactg
    27961 acctatacct ttgcattttt tactcttaac aaaaaataag gaactgtgac aaaatcgtgt
    28021 cacagtccct tatttcgaat aaacaatttt ctagaagtag cttcttcaga aatgatttat
    28081 tacttgattt ctattctttc gcttctgtcg ctgtacctgt gatcaattcg attgcttttt
    28141 tcattgtcac atcatgttcc agcatatctt ctgtcaacac gcgtttgatt tgttcgattg
    28201 gcatgttgta tgtttctgat aattcttgga tttctgcatt gatttcttct tctgttgctt
    28261 gaagattttc agcagctgca attgcttcga tcacaagatt tgttcttgtg cgcatttctg
    28321 cttcaccttc gaattgttta tgcaagtctt cttctgtaga accagttaat tgatagtaca
    28381 tttcaggaga gataccttga cgttgcatgt tgttcaagaa ttcgtccatt gaacgatgta
    28441 cttcatcatg aaccatcacg tgtggcaatt caacgatttc tgcgttttca acagccatac
    28501 ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc ttttgtttct actaattctt
    28561 tgcgatattt ttctttcaat tcgtctaatg ttgatacttc gtcatcaaca tcttttgcga
    28621 attcgtcatc taattcaggc acttcttttg cttttacttc atggattgtc actttgaaga
    28681 ctgcttcttt tccagcaaga tcttctgctt gataatcttc tgggaaagtt acatttacat
    28741 ctagactttc acctgattta tgaccaacta attgatcttc aaaacctgga atgaatgagc
    28801 ctgaacctaa ttcaagtgag taattttcgc cttttccgcc atcaaacgct tcgccatcta
    28861 caaagccttc gaagtcgatt actactgtat cgccattttc agctgcagca tcttctttga
    28921 taactaattc agcttgtgct tcttgttcac gtttgatacg tgcatccacg tcagcatctg
    28981 ttacttcacg gtcttgtttt tcaacaacta gatctttata ttgtcctaat tttacttctg
    29041 gtttcactgt tacttcagca gtgattaccc agtcttgacc tttttccatg ctttcaacat
    29101 cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt gacagcagct tcataagctt
    29161 ctggtaatac agcattcaat gcatcttcat ataaagcttc ttcaccgtac atacggttga
    29221 atacttgacg agaaactttc cctttacgga atccaggtac gttcaagctt tttttcactt
    29281 tattaaacgc agttgttaat cctttttgga tttcgtcttg cgcaatcgta aattttaaaa
    29341 caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat ttaattccct ccgagtttag
    29401 aaattcattt tatacatcaa aaacgtttca tgcatacctt taaatagtac actaaccctt
    29461 tcaaattgta aacatattga atggatagaa tccttgtttt tcataacatt tttatctttt
    29521 tcactgataa aatctaatgg aagaagatca tttgctgcaa aattttttct ctttttttta
    29581 aaaaatcttg tttttcatct tctgtacctt catacatcgt ttttcctgta cacgcctcta
    29641 cagtcagttg aaaccagcgc ttcgggtctt cgatgtatgt atcatagaca ggataaacaa
    29701 ttgcagattg caacgtgaat tcttctttta acacggaact aagcagcgca tcttcattcc
    29761 cgtaacgatc tgaaaataca cataattgct ggtaacttgc ctgctgctgc ggctttggca
    29821 gatcttttgg tatgactttg actaactttt cttcaatcgt caaatagaaa acaggatggc
    29881 attcgttcaa ttgcctaagt gtctccagta attttgccct tgctaaaata tggacctctg
    29941 gcgatacaat aagtttactc gctgtttgga taaactcatg ataaggaagc tgttcgatct
    30001 catcaataga cgcaagctga cgtgcaggtt ccaacgaagg aagttcctcc gatttattgt
    30061 ggagtgcctt gatcttctgc ctctccattt gtccggataa ttcttctgca aattggattt
    30121 tttcaatgac ccgctgctcg aaagcttcat aaaaatctgt acttgctatc aattttctag
    30181 catataaaaa atcttgtgta ctgattaaca agtcaaaata aaattcagca aactcctcat
    30241 tttgaagata ctgtttttca tgcaagagtg cttgcttcaa tgcttctttt ttctcgtcca
    30301 actcgtaaag acaaaaaaca agcaggctat tagcctcaaa gctttctttc aactcgtaag
    30361 ctcgttgaaa taattctttt gcctgctgat attgctggtt tgctagtgct tgttgtgcca
    30421 ctcgcattaa attctcatat ttttctggaa aatcaatcgg cttcacgcag tgtcctcctc
    30481 ttgctcatat tcagctcatg ttcaatagta gcaaaacaaa aaaagcatga caattaaaaa
    30541 taactgaagc accctctaaa agctagattt tagatctgac ttttagaagg tgctttcaac
    30601 tgttactgct ttttattgct catcagctgt tgacttcctg gttgaacgct ttgatcagat
    30661 tgatcgtaac aacacttgta ttagtgaaaa aatcgcccag atgttgcttt ttctgattaa
    30721 agactgctgg aagataccct aacatcagca aaggaaattt tagaatgaac cggcaagcac
    30781 cttctctaac taaaacggtt gaccagctaa gttcttcttc atcaaagctg atgactcgaa
    30841 taccaaagat cattttccca atcgtttggc catgattgaa tttcgtcaat aaaataaaat
    30901 acgctagata aataagtaag gctgtgaatc cgtagagact cagataagaa tctgaaaaat
    30961 taagccaacc tagatttttg gcaataccaa gtgttaaacg cgtaatagct ccgatacaca
    31021 aaagatcgac caagaaggca aacattcgga taaaaaaacc tgcatagaaa tacttaggaa
    31081 aatcatttgc ttttggcggt tcatttcttg tgtccgtata ctttttccat ttacgttgtt
    31141 tctttttgat ttcttcgggc gtcaaaggct ctctttcaaa agagctcagt accttttggg
    31201 aaaagttgga gtcaggtcct acagcaggct gctgttgctt atctttttct tgttccttat
    31261 tttcatttga aagctctgac atgcttactc acctccgtaa taatacattg cttttggtga
    31321 ttccgctgtc cctagattct caagaacgga taagatctga ctagtctcag aaggcttcaa
    31381 tccctggaat tctgctagtt tacttcctaa ccatgtacta gcaaacccag atggcgtgga
    31441 agaatattct actagttccg catcttcaag ctgttgttcc tttcgtatag cttttaaagc
    31501 atcttcagga aatcccaatt catcgactaa acctacttct tttgcttgga caccatcata
    31561 aatccttcca tcagcaattt ttttgacttc ttcttcagat ttattccgtc cctcgctgac
    31621 gattgctaca aaacgactat atgcgctgtc gatatatgct tgaagcaccg cttcttcttc
    31681 ttttgtctct ggtcgcgttc ctgaacccat atctttcaac gctccgcttt taactgttgt
    31741 atcttccaca cccagttttt ctagaagtcc tgaataattc aatcctgaca tgatcacacc
    31801 aattgagccg gtcactgttt cttctgttgc aaagatttta tctgcttgag cagaaatata
    31861 gtagccaccg ctagctgcca tgtttttcat gctgacatac attgggatat catgttcttt
    31921 acgaatcgta tccaatttct tagcgatttc cgcactttcg tagacaccgc caccaggaga
    31981 atttacttct aaaaagatac ctttgactgt tggatcttct tcaatcgcct tcagttgttc
    32041 cataaaattt ttatgattgt aaccttcaga agcaaataaa cctgaagacc ctcccgcagc
    32101 aatcgttcca tctactgata attttacgat tttttgcgta ctgtctccct cttccaacac
    32161 atttggagct agttcttcat ccccatataa ccaactgttc aatccctcaa tttgatcgtt
    32221 ttcctgaggc ttagtcaaac tagctgaaac taacgaaacc acaaataatc ctgcagcaat
    32281 cagaactgct gcccaacgtc ttttattcat tctttctcct ttctaataca agttttttat
    32341 gacgatcaca gtttctgact gctcagaagt gtcgaaccgc tctgcttttt cttcccgcgc
    32401 atacccggaa aacgactctt ttgttaattc atcttctacc gtaaactcac gagcttgagc
    32461 aacttccaag gcatcaggtg cgatttcttg gatgctatcc cagccagtat ctaaaaatat
    32521 tttcgtatca tctgatacac cagctgatgc ttcgaatttg gacaattgtt tttttaacat
    32581 tcccagtgtc atgatctcaa tttttgaatc catttttact cctcctctat ccgattactt
    32641 tctattgtac cgaactcctg aaaaagatga tagataaaaa cccattattt tttgacataa
    32701 aaaggacatt tgacaaagaa tttgatatat accaatttat tttttattct ttttttgttg
    32761 acaagaacat tcgcaaaagg ttatgatttt aaatgtttcc agataaaaaa caaaaaggag
    32821 gatagttata ccttttagga tagcgccagg tctgtgtaaa cagatgacga ggagagggtt
    32881 tatcgaaaga ttcggcggat ggccctaggg actgcactct acagaaaatc cacaaaaaat
    32941 gtctgcaaag ataaaacaaa aggattttcc tgcagctgga agcattcata cgaattcatt
    33001 atagaaagta ggatgttttt tatgtgcgga attgttggaa tgattggatt aaaaaatgta
    33061 acaccaggat tgattgacgg tctagaaaaa ttagaatacc gtggttatga ttcagctggt
    33121 atctttgtct cagatggcac aactgattat ctggtcaaag cacaaggcag aattcaaaat
    33181 ttgaaaaaca aaataaatat tgatactaca ggaaatatag gtattggtca tacacgatgg
    33241 gcaactcacg ggcaaccttc tgaagaaaac gcccatccac atacttccca aagtggacgc
    33301 ttcgtgctcg ttcacaacgg cgtaatcgaa aactttgaag aacttaaaat tgcttattta
    33361 gcaaatgacc actttattgg tgagaccgat actgagatta tcgctcatct aattgaaact
    33421 ttcgcgaagg atactacaac gcaagaggcg tttttaaaag ctttgcgggt catcaaaggt
    33481 tcttacgctt tcgctttgat tgatcggaca gcacctgatg ttatttacgt cgctaaaaat
    33541 aaaagtccac tattaatcgg actcggcgat ggattcaatg tgattgccag cgatgcaatg
    33601 gctatgcttg ctcatacgaa ggaattcgtt gagattgaag atgaagagat ggttaccgtc
    33661 acatcagaaa aagttattat tcaaaatttt gcaggtgaca tcgtagaacg ttcttcattc
    33721 gaagcacaag tagatgcttc agatatcgag aaaggtacct atccttttta tatgctgaaa
    33781 gaaatcgacg aacagcctat tatcatgcgt cgtatcgctc aaaaatacgt aacagaagac
    33841 aatcgtgtct ctcttgatca aaagctggtc gatacattgt ctgatagcga tcgaatctat
    33901 atcgttgctt gcggaactag ttaccatgcg gggttagcag gtaaacagac tttggaaaaa
    33961 ttgacacaga ttcctgttga ggttcattta gccagtgaat ttggctataa cacacctctt
    34021 ctttcaaaaa aaccattctt tattttcttg agtcaaagtg gcgaaacagc agacagccgc
    34081 caagtgctgg taaaaatcaa ccgtctcggc tacccttcac ttacgattac aaatgttgct
    34141 ggttctactc tttcgcggga agcatccttt acactgctat tgcatgctgg acccgaaatc
    34201 gcagtagcat caacgaaagc ttatactgca caaatagctg tactaacttt attagccaaa
    34261 gctatcggtg ataaaaaaga atttgaaact tctcttgtat tcgatatcac tcatgaattg
    34321 agtttagttg ctaatgcgat ggaatcggtc atcgcccaaa aagattattt agaagagttg
    34381 gcagaagtat atctcagcga tacaagaaat gccttttaca ttggacgtgg tgctgactac
    34441 aacgtttctc ttgaagctgc tttgaaattg aaagaaatca gttatatcca ggcagaaggt
    34501 tttgccgctg gcgaattgaa acatggaacg attgctttga tcgaagaagg aacaccagtg
    34561 atcggaatca tcagtgaaga agtgactggt gcgcatactc gaggaaacct gaaagaagtc
    34621 gaaagccgtg gcgctaaaac actagttatt gtctcagagg gattagaaaa ggaaagtgac
    34681 cagctggtgc taccaactgt ccacccttat ctaactactc taaccacagt cgtaccgact
    34741 caattacttg catattacgc aacgctatta cggggattcg acgttgacaa accacgtaat
    34801 ttagcgaaat cagtgacagt agaataataa aagaacaagg accagtgtca gacatctgac
    34861 actggtcctt gttcttttat tttttatgaa aatatctctt aatccagtcc gatttcttct
    34921 ttgactactg ccgcgatttt atcgacatag taattaactt tctcatcagt aggcgcttct
    34981 gccatcactc gaagtaatgg ttctgtaccg gaaggacgaa ctaggattct tccttctgat
    35041 cccatttcag cttctgcctc ttcaatcact tttttgattg ccggaacatc catcgcaccg
    35101 tttttatcac ttacgcggat attcactaat ttttgcggat agattgttac ctctgctgca
    35161 agctctgata atttcttacc tgtctgtttc atgatattca gtaactgaat tcctgaaagc
    35221 atcccatctc cagttgtgtt aaaatctaag aaaatcatgt gcccagactg ctcgccgcca
    35281 aagttataat cgtttttgcg catttcttct acaacgtaac gatcaccaac ttgtgtaacg
    35341 acatctttca aaccgattcc ttccaccgct ttgtggaatc ctaagttact catgacagtc
    35401 gtaacgatcg tatctttttt caatcgattt ttttctgcca agtatttagc acagataaac
    35461 atgattttat caccgtcgac aatgtttcca agttcatcca ccgcaataat acgatcgcca
    35521 tcgccatcga aagctagtcc agcatctgct cctttttcaa cgaccatctc agctaaacgt
    35581 tctggatggg tagagcctac gccgtcattg atatttaacc cgtctggatt tgtgcccatt
    35641 gtataaaaat ctgtttcaag atcggcaaac aaacgattaa cacttgttga agttgctccg
    35701 tttgctgcat cgacacaaat ggtcaaaccg gaaaggtcac cgttgatcgt ttgtacaagg
    35761 aattgagaat atttcaataa gccttctgga aattcttcta cagttcccaa tccttcagct
    35821 gatggacgag gcaattcatc cacttcagca tctaataatg cttcaatttc tagctcttgg
    35881 tcatctacta gtttaaaacc atctgcaccg aaaaatttga ttccattatc ttgtgctgga
    35941 ttatgagaag cagaaatcat gacccctgcg cttgcttttt gcaaacgtgt cagataagca
    36001 acacctggtg tagagatcac acctagctgg aacacttcga tacccaccga taaaagtcct
    36061 gaaatcagcg cttcttctaa catttgacct gagattcttg tatcccgccc tactaacacg
    36121 cgtgggcgtt ttccttcttc ttcatgctgg cttaggacat aacctccgca ccgtcccagc
    36181 ttaaatgcaa gttctggcgt aagttccttg ttggcttcac cgcgtacgcc gtctgttcca
    36241 aaatatttac ccatagttgt aaaattactc ctttaactta attttctgag gaatttgttc
    36301 tgctcatttg tgttgattcg cttgtttctg atgtttcttc tgtacttgtg gtcgagctgc
    36361 ttgtcccaga ttccgaactg gaagaaatgg ttgttgtcga tggttccgtt cctgaatcag
    36421 cagatgaaat acttctactc gtacttgtat tttcttgtgt ccgactgctc gtcgaagcag
    36481 agctactgct tgacgtttgc aacacgggtg taatctccac actaactgat tcgggcttga
    36541 tacttaaccc ctcttctact ggtatttcaa ttttacgagt tgttcgatga ttgatacctg
    36601 agacattgat cggtacagca atactgtcac cgatcgatgc aagcaaatcg ctagagccgc
    36661 ttacttctgc ttccagtttt gaaaaactga aatcatagct ttctaccccg gcagctggtg
    36721 ttccttgctg tatcccataa agtcgtaccg acttgcttgg cttgatcgaa tcagctctca
    36781 ctgttacttg cactggatca gagacgatac ttaatacttc ccctgctgca ttcagcgcct
    36841 gtattgggac ctcactatta atcccatcat ctgtgaccat cgaagcgtcc acatcagcta
    36901 ctactcgttt gatttctgcc aatgttttgt cacctgtagt gatagagact tttttaggac
    36961 taacggaagt atcatcaagt tcatatccag atggtgtgaa cgttgaagga atcaacgttt
    37021 ccacatcgaa atcttttatt acctttttct caaccgttac tgtaatagtc gatggttcga
    37081 tctttgctgt gacagcactg ctcaaatttt gaatttttag cggaacttca tgtgttcctt
    37141 cgcccagctt agtgatatct gctgtgacgc ggaacatccg tgtatcttcg tttgcctctg
    37201 cattcaactg aactcggttt gcactggaaa gtttgacagt cactgcattt tcatatccat
    37261 ggatatagta gcgctttgaa tcataagaaa tcttgatcgg cacccccgta actgtttgat
    37321 tatacgcttc atttccagaa agcgtatttg gaacggactg gccatttgca ttgaagtaca
    37381 gcgcaatact gaacagcaat gcaataaaac tgtaaagaat gcttgtccgt ttctcttttg
    37441 agatcatttg cgatcccctc ccttagtcat accgtctagg aaagattgaa tcacgttttt
    37501 cttgtttttt gttttctctt cagcaattag ttctctacgc aagattgcca aatattcttg
    37561 ctgggataag ccagccatca gttcattatc taacgtgatg ctgacgtccc cggtttcttc
    37621 agagacaacg atggtaatcg cgtcactgac ttcgcttata ccgacagctg cccggtgtcg
    37681 agtcccgaat tctttaggga tcaacatact ttctgataga ggaagatagg cgcttgctac
    37741 tgcgatcttt ccttctttta caataactgc accgtcatgc agcggtgtat taggaataaa
    37801 gatattgatc agcagctccc cagtgatatc tgcatctaaa gcaatccctg tctcaatata
    37861 ctcatctaat ccagtatgac gttcgatagt gatcaacgca ccgatttttc gcttggacat
    37921 atattggatg gctttgtcaa aagacaagat catcttctca tcttcctgct gttcgctttt
    37981 tgattgtttg aaaagcgaac tgcgtccaag atgttccagt cctctccgga tttccggctg
    38041 aaaaatcacg atagcagcta tcaccccgta cgtaatgact tggttcatca gccaagacaa
    38101 ggtgtgcaga ccaatgattt ccgctacgat tcggataaca ataaaaatag ctacaccttt
    38161 cagaagctga atcgcttttg tgcctcgcag cagttggatc agtttgtata caagatacca
    38221 gacaaccaga atatcaagta tgttcaccaa aaaattcaga gagaacaagt caccggaaaa
    38281 catttgctgc caatagtcca aattaaaaag ttccccgatt tgaaatgaca taaaaagcct
    38341 ctcttctctt ttgagatttc ctctctagta tatcatatgt tttaatttta gaggctgata
    38401 tgctcgtatt tttcttgaga aaaacgcata attttcaaga aaattgcaag tatgcgtttt
    38461 atttttctct gcaataaata tttttaaact tcagagaatt attcattata ttttttctgt
    38521 tatttttcct ttttttcttt tgtagcttgt tccatttttt tacgtcgttc tgctctggca
    38581 gctcttctag cagctcttct ttcttctgct tcaaggtccg tcgttttttc ttttctagtc
    38641 ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt ttctaattct
    38701 cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cgtggacagt cttttcaaat
    38761 aaagcttttg caagatttgg atttgcgtta tctagcgagg aaaagcgaac ttgtttcctc
    38821 atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat tgggttcttg
    38881 tttttttcga ccattgtagg gttatatcga tataatgacc aataaccaga agaaacagct
    38941 tcttttgctt ctttaagcga ttctttcatt ccgccaagca agccatgtgt gatacaaggc
    39001 gtataggcaa taatcagaga aggaccagga tacttttctg cttcttcaaa tgccttgatc
    39061 gtttgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc atatgtcatc
    39121 gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa tttagcgatt
    39181 gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac ttcgttatcc
    39241 aaaacaagga tattcacgtc tgctccgctg gcaagtacat gatcgatccc gccaaaacca
    39301 atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac gaatagatcc
    39361 tttctcttgt atatatcctc cagttcaggg aaaattgatc gttcgctttc gatcgcagct
    39421 tgcagttttg ctgctctttg ctgcgttcct tcactctcaa acatgtgtac taaccagtct
    39481 tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat tgaacttgcc
    39541 aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc agcattatct
    39601 tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt ataaggagaa
    39661 gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat catcattcga
    39721 tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc tgagcaagcg
    39781 cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt ctttcctttg
    39841 actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc ttgttctttc
    39901 tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc ctctacacac
    39961 agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac gccgtctgcg
    40021 cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc ttcatctgct
    40081 aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca catcgtacag
    40141 cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat agaagccgtt
    40201 cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac agagagttga
    40261 tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt gttcctttta
    40321 ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac ttgatgaagc
    40381 agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat tgatttatgt
    40441 ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa aggaatgatg
    40501 tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc caaaccgact
    40561 tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc agccaagtaa
    40621 cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag agtattcaat
    40681 aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag gtaagctggt
    40741 gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat cggcgtatct
    40801 ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata ataaaaatag
    40861 ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt atttgctcca
    40921 actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg ttctgtcaaa
    40981 tccagacttt cctttggttc caaagattga taagtcacat catccacgat ccctatggta
    41041 aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc tgagatttgg
    41101 tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc cgggcgaagt
    41161 tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc tgcccctggt
    41221 tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt ttgcacgaat
    41281 gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt ccgtccgttt
    41341 gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat agaaacaatg
    41401 acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt tcctcgtaat
    41461 cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata ataggggttg
    41521 atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact aacagtcgga
    41581 tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt gatcagagga
    41641 gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt acggaatcca
    41701 tcaaagaaat tcaaaaaagg gacttctcct tcaatagaag caagatgggc aacaggtgct
    41761 aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt ttgtcttgca
    41821 gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac tgcccgacta
    41881 gcaacgtgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt cggtatcatc
    41941 aaaagcagcc cttgtgaagc ggtatatgta gttgtcagtg caccagtttt caatgatcca
    42001 tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac tggttgaccg
    42061 aatacgtttt cttttcctgc tgctgaccat tgatctacta attcagccat ggtcgaactg
    42121 ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata tgcggctgcc
    42181 gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc atattctctc
    42241 ttattatctg ttccattata acgtaaaaca tgccattttt ttcttttatt tataaaaata
    42301 ttttacatgt atctatttga attggtcatc taatttttag acaaacagtc taaatcgttg
    42361 cctttttttg tcagacatgt ataaactata agcgaaagag gtgttttaac atggatatat
    42421 cagtgattga tgcaacgaaa acaaatacac aaaaaggaat cctttatggt tcatcgaatg
    42481 ctcctaaaaa aatggtcgaa ttcatcaact tagcttgtcc gtattgccga caatggtttg
    42541 aagaatctta tgagctatta gaagaagcag tccaaagcgg gcaattgcag cgagtgatca
    42601 aattgttcga taaagaaaaa gaaagcttac agcgagggaa cgtgatgcac cggtatctca
    42661 caatcagcga tggccaaaaa gcgatcaaag agatcaaaca aatattcgat actcaagacg
    42721 agtggaaaca tctttctctc caagaagtag cggattttgc agtagataaa ctaaagctga
    42781 cagaacaaaa agacgaacag ctgagccaag ctgtcatcaa cgaagctgag caagcacata
    42841 ttcgatttgt tccaactgtc atcttgggta aagaaatctt tgatgaatct atttcaatag
    42901 aggaattaaa agaattgatc caaacaaaat aaaaaaataa ggagcgtg
//
LOCUS       DAPCMT010000011        41034 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPCMT010000011 DAPCMT010000000
VERSION     DAPCMT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 41034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.011"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(38..1189)
                     /locus_tag="RD743_000462"
     CDS             complement(38..1189)
                     /locus_tag="RD743_000462"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDU0562320.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="RD743_000463"
     CDS             complement(1202..1639)
                     /locus_tag="RD743_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0562321.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="RD743_000464"
     CDS             complement(1654..3420)
                     /locus_tag="RD743_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDU0562322.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="RD743_000465"
     CDS             3684..4361
                     /locus_tag="RD743_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562323.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="RD743_000466"
     CDS             4364..5491
                     /locus_tag="RD743_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562324.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="RD743_000467"
     CDS             complement(5691..6131)
                     /locus_tag="RD743_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0562325.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="RD743_000468"
     CDS             complement(6142..8175)
                     /locus_tag="RD743_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDU0562326.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="RD743_000469"
     CDS             complement(8177..9880)
                     /locus_tag="RD743_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0562327.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="RD743_000470"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="RD743_000470"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDU0562328.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="RD743_000471"
     CDS             complement(10963..11988)
                     /locus_tag="RD743_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDU0562329.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="RD743_000472"
     CDS             complement(12197..13066)
                     /locus_tag="RD743_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDU0562330.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="RD743_000473"
     CDS             complement(13525..15417)
                     /locus_tag="RD743_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562331.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="RD743_000474"
     CDS             complement(15414..17141)
                     /locus_tag="RD743_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562332.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="RD743_000475"
     CDS             complement(17157..17807)
                     /locus_tag="RD743_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDU0562333.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="RD743_000476"
     CDS             18011..18307
                     /locus_tag="RD743_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDU0562334.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="RD743_000477"
     CDS             18479..19099
                     /locus_tag="RD743_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDU0562335.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="RD743_000478"
     CDS             complement(19191..20357)
                     /locus_tag="RD743_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDU0562336.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="RD743_000479"
     CDS             complement(20369..20722)
                     /locus_tag="RD743_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDU0562337.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="RD743_000480"
     CDS             20850..21872
                     /locus_tag="RD743_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDU0562338.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="RD743_000481"
     CDS             21891..22052
                     /locus_tag="RD743_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562339.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="RD743_000482"
     CDS             complement(22311..22745)
                     /locus_tag="RD743_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562340.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="RD743_000483"
     CDS             complement(22855..24714)
                     /locus_tag="RD743_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDU0562341.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="RD743_000484"
     CDS             complement(24832..25992)
                     /locus_tag="RD743_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDU0562342.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="RD743_000485"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="RD743_000485"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDU0562343.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="RD743_000486"
     CDS             complement(27739..28437)
                     /locus_tag="RD743_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDU0562344.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="RD743_000487"
     CDS             complement(28394..30055)
                     /locus_tag="RD743_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDU0562345.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="RD743_000488"
     CDS             30273..31673
                     /locus_tag="RD743_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDU0562346.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="RD743_000489"
     CDS             complement(31925..33004)
                     /locus_tag="RD743_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0562347.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="RD743_000490"
     CDS             complement(33090..34049)
                     /locus_tag="RD743_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDU0562348.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="RD743_000491"
     CDS             complement(34061..35572)
                     /locus_tag="RD743_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDU0562349.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="RD743_000492"
     CDS             complement(35594..36157)
                     /locus_tag="RD743_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDU0562350.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="RD743_000493"
     CDS             complement(36163..37026)
                     /locus_tag="RD743_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0562351.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="RD743_000494"
     CDS             complement(37040..37936)
                     /locus_tag="RD743_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0562352.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="RD743_000495"
     CDS             complement(38111..39400)
                     /locus_tag="RD743_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0562353.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="RD743_000496"
     CDS             complement(39372..40889)
                     /locus_tag="RD743_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDU0562354.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11840 a         8591 c         7097 g        13506 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata actt
//
LOCUS       DAPCMT010000012        37283 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPCMT010000012 DAPCMT010000000
VERSION     DAPCMT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.012"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            11..877
                     /locus_tag="RD743_000497"
     CDS             11..877
                     /locus_tag="RD743_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDU0562355.1"
                     /translation="MAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELT
                     KENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPG
                     VYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVL
                     QGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFK
                     IEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLV
                     FISIKFRKKRNI"
     gene            complement(1301..1471)
                     /locus_tag="RD743_000498"
     CDS             complement(1301..1471)
                     /locus_tag="RD743_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0562356.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1665..1844)
                     /locus_tag="RD743_000499"
     CDS             complement(1665..1844)
                     /locus_tag="RD743_000499"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562357.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2231..3343
                     /locus_tag="RD743_000500"
     CDS             2231..3343
                     /locus_tag="RD743_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDU0562358.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3421..3714)
                     /locus_tag="RD743_000501"
     CDS             complement(3421..3714)
                     /locus_tag="RD743_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDU0562359.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3882..4196
                     /locus_tag="RD743_000502"
     CDS             3882..4196
                     /locus_tag="RD743_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDU0562360.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4217..6196
                     /locus_tag="RD743_000503"
     CDS             4217..6196
                     /locus_tag="RD743_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDU0562361.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6209..6535
                     /locus_tag="RD743_000504"
     CDS             6209..6535
                     /locus_tag="RD743_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDU0562362.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6551..7069
                     /locus_tag="RD743_000505"
     CDS             6551..7069
                     /locus_tag="RD743_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562363.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7069..8421
                     /gene="celB"
                     /locus_tag="RD743_000506"
     CDS             7069..8421
                     /gene="celB"
                     /locus_tag="RD743_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDU0562364.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8505..10010)
                     /locus_tag="RD743_000507"
     CDS             complement(8505..10010)
                     /locus_tag="RD743_000507"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDU0562365.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10102..10692)
                     /locus_tag="RD743_000508"
     CDS             complement(10102..10692)
                     /locus_tag="RD743_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDU0562366.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10871..12517
                     /locus_tag="RD743_000509"
     CDS             10871..12517
                     /locus_tag="RD743_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDU0562367.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12566..13285
                     /locus_tag="RD743_000510"
     CDS             12566..13285
                     /locus_tag="RD743_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDU0562368.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13764..14711
                     /locus_tag="RD743_000511"
     CDS             13764..14711
                     /locus_tag="RD743_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0562369.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14723..15643
                     /locus_tag="RD743_000512"
     CDS             14723..15643
                     /locus_tag="RD743_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0562370.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15684..17141
                     /locus_tag="RD743_000513"
     CDS             15684..17141
                     /locus_tag="RD743_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0562371.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17486..18100
                     /locus_tag="RD743_000514"
     CDS             17486..18100
                     /locus_tag="RD743_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDU0562372.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18093..19817
                     /locus_tag="RD743_000515"
     CDS             18093..19817
                     /locus_tag="RD743_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDU0562373.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19814..21265
                     /locus_tag="RD743_000516"
     CDS             19814..21265
                     /locus_tag="RD743_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0562374.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21738..23891)
                     /locus_tag="RD743_000517"
     CDS             complement(21738..23891)
                     /locus_tag="RD743_000517"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDU0562375.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23893..24939)
                     /locus_tag="RD743_000518"
     CDS             complement(23893..24939)
                     /locus_tag="RD743_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0562376.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25089..26378
                     /locus_tag="RD743_000519"
     CDS             25089..26378
                     /locus_tag="RD743_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDU0562377.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26391..29093
                     /locus_tag="RD743_000520"
     CDS             26391..29093
                     /locus_tag="RD743_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDU0562378.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29078..29935
                     /locus_tag="RD743_000521"
     CDS             29078..29935
                     /locus_tag="RD743_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0562379.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30006..30905)
                     /locus_tag="RD743_000522"
     CDS             complement(30006..30905)
                     /locus_tag="RD743_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDU0562380.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31074..33245
                     /locus_tag="RD743_000523"
     CDS             31074..33245
                     /locus_tag="RD743_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDU0562381.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33313..33654
                     /locus_tag="RD743_000524"
     CDS             33313..33654
                     /locus_tag="RD743_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDU0562382.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33930..35684)
                     /locus_tag="RD743_000525"
     CDS             complement(33930..35684)
                     /locus_tag="RD743_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562383.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35695..36531)
                     /locus_tag="RD743_000526"
     CDS             complement(35695..36531)
                     /locus_tag="RD743_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDU0562384.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36533..37216)
                     /locus_tag="RD743_000527"
     CDS             complement(36533..37216)
                     /locus_tag="RD743_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDU0562385.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12029 a         6557 c         7651 g        11046 t
ORIGIN      
        1 agatggaaaa ctggcaattg acggtttagc accaggagca tatcagctgg tagaaactca
       61 agcgccaacg gggtatgaat tagatgcgac accgatcgag tttgagatcg aaagaagtca
      121 gacagcggtg gtagaattga cgaaagaaaa ccgacttaca ccaggcggtg tcgtgttgac
      181 aaagatcgat gaccaaagcg gggaagtcct gcaaggagct gtttttgagt tacaagatgc
      241 caatggaacc gttctgcagt caggtctaac cacaggagca gatggaaaac tggcgattga
      301 cggcttagca ccaggagtgt atcagctggt agaaacccaa gcgccaacgg ggtatgaatt
      361 ggatgcgaca ccaatcgagt ttgagatcga aagaagtcag acagcggtgg tagaattgac
      421 gaaagaaaat cgacttacac caggcggtgt cgtgttgaca aagatcgatg accaaagcgg
      481 agaagtcctg caaggagctg tttttgagtt acaagatgcc aatggaaccg ttctgcagtc
      541 aggtctaacc acaggagcag atggaaaact ggcgatagat ggcttagcac caggagcata
      601 tcagctggta gaaactcaag cgccaacggg gtatgaattg gatgcgacac cagtaacttt
      661 caagatcgaa aaagaacaag aagtggcgat ctttttaaca aaagagaata gaaaagttgc
      721 tgatagctca gatataagag gaaacaatcc aacaagcagt ggaaataagc atcttccaaa
      781 aactggagaa accatcatta gccagtttat attgtcaata ttgggtgtgc tacttgtttt
      841 catcagtata aaatttagaa agaaacgaaa tatatgagct aaaataaaaa attgaataag
      901 catagttttt aaaatcaact agtacccatc aattgaatgg aaactaccga ttttagaagg
      961 ggtatacaaa caaatattag tatcggagta gagaaggttg aacccaacca gtaaataagt
     1021 aaagacaatt ttgtgctgcg ttgagaagtg aaaaataaac ctttgtagta atactagtta
     1081 ataaaaaata tacaaaaaca ctgcgaaaat cattcttgag tttcgcagtg ttttttgcct
     1141 aatgactcaa gtttattggg taatactttg attttctttc tacaaataat cagcgatcag
     1201 ttgtaatttt ttctctagtt ttaatggctc tttgtcaata aggagaaaaa caagtcgggt
     1261 cttttgtctc ggaaagcttc aaccaactcg tgaataaagg ttacgcctcc ttgaaaggcg
     1321 tggaaaaacc aagtaatcga tcaatcacca atttcgcatt tggtagaaca tgtttggtga
     1381 gctgaaagta ggtggcgttc atgtctgtaa ctaagaattc tatttcttta ggattagtgc
     1441 agctaaggaa atagtttgtt aatcgaggaa ttttttgtgt aggtaaattt tctcgattaa
     1501 tttttctgtc tcgccatccg cggaaataaa actcagttta tcctcaacgg aaacgtgaga
     1561 acgaaattca caaccctcaa cactcaagac agaattcttt tggaagactt tggtaaatag
     1621 cttctcaact ctttcaatgt gcgaataacg gttgtcaaag atacctaaca acttttcgaa
     1681 ataaaaaata acgacacttt ttcagtcagt aaagaagtga ttttatgtct gacatgattt
     1741 gaaatcgagt gccttggacg gataaaataa ctttgagcgt tccaatggct tcgacagttt
     1801 ttacaggtat agcgctgctt ttttaggcgt ataaccattg gcatatgatt atattattca
     1861 aagtggacaa tggtttcctt cttcttgttt ttcaccacca ttaatttcct atttccatct
     1921 accacagcag aaccacaatt tctacagaca catggagcag ggaagagaac tgcagcaacg
     1981 atcaatgttt ttttcttctg taaggtttcg taagagacct ctgtaatcat caaatttttg
     2041 tctattgatc tcagaatttt tttgatagaa ccattcataa agcatatcgt tctctcagtt
     2101 gtttattttg tggtgattta atcatacaag aaaacgatat gtttttttat aaaaacatga
     2161 ataggaaaaa tagttgacaa gaaaatgaca acgccatatg atgttccata gtatttacaa
     2221 cctgtcatag atgactgtaa aaaataaaag taaaaagaaa ggacgacaac aggtggattt
     2281 ttatcagtct ctacaactag atccgtttat tttaaaacaa aaaatacgag aagcagcttc
     2341 taaaaaagaa aagtgcatgt atatttgttc attgttttta cgatcattat tcatcgtatt
     2401 gtttgccatt tgttttatca ttataattac aacattattt gaatcgaagc ataaaccata
     2461 cgctgtggtt ttattctgta tgttgatgag tattcgtttt gttgactttg gttataagat
     2521 ttcccattcg atcataggtt tagcaatcgt catgttctca ctattagtag caccgtatgt
     2581 ccagctgatc aaatggtcag ttatgggcat gttgattcat tttattttac taagcagcat
     2641 attaatggca accgctagtg atcccaaaat ggggaatgct tctttgtacg gcttttcata
     2701 tctctttatt gtttattcgt tacctaaaga ttcgttgaac aagaatttct ttacacaaac
     2761 aagtagctta ttatttttgt ttttttgttg gttttcagtt ctcctttaca gaaaacatcg
     2821 agaaaagaac aaagacaaaa gcttgtttaa agagattttt ttgaaaggca tgtattcgca
     2881 ggaaaaaatt tggatgctca tctatgcatt tggcattagt cttttgatcg ttgcaggaga
     2941 gtatgtccct tttcagcgat tgatgtgggc agggtttgca ttttcttcta ttgtatctag
     3001 ctatggattg atgtcgattg gtttcaaaga acgagcagta gaccgaatca ttggctcatt
     3061 gattggatgt gttttattta ttggtatcag ccaatttatt ccttttaact gggtcggtat
     3121 attaggtggt ctagctttag gtgtttgttc tacctataga tataaaacag tttttaattc
     3181 ttttggtgct ttggcaatca ctgccagttt attcggggtt ccaggagcag taacgatccg
     3241 catttttgag aatatccttg gggtatgttt aggaatcatg tatattgggg tgactgaaat
     3301 acttatccga aaaataagag aaaaacacgg tttgaatcat tagatgagat cgaataaaaa
     3361 ataaggaaaa agtgaagata cacatggatg ataaattgct atcattccat gtgtatcttt
     3421 ttacggtaaa tcagtgaaac gttcgacttt catttttaat ttatactttt tacggagttc
     3481 gtttatttga gccatcattt cggatttgtg atgcttttcg attgcttctt catctttcca
     3541 acggtcgatc agtaaaagag attctggatc ttctagtgga aagtaatagt catatttctc
     3601 gtttccttct tctgcacgaa tcgctttgac aatgtttctt tcgaccattt cttctgcaaa
     3661 ttttctggct tgtccatttg tacttgtata caaaatattc actgtgatac tcactaaatc
     3721 ccctcctgaa aaaattctat cactagtata tcacggtgcc agaaattatt attcaagata
     3781 tgatacacat tttttccgca atgctacgga aatatatatt ttgaatagaa aacgcttaca
     3841 agatatgctt ggtttataaa aataaaagga gtgattgatt tatgaaaaaa gcattgatta
     3901 tttgtgcggc aggaatgtca tcctcgatga tggcctcaaa gacaacggat ttctttaagc
     3961 aaaaaagaga agaggtactc gtagatgcag tttcagctac agaaggcgat aatatgatta
     4021 agaatagtga ttttgattta tttctgatca gcccgcaaac gacgatgttt ttggataaat
     4081 tcaaaaaatt aggagaatct gttggaaagc ctgttgttag tatccctttc caagcgtatg
     4141 tcccaatccc tacaggcatc cagaagttag cagaggtgat cgaagagaac atatgatgga
     4201 ataggagtgg atgataatga aacagaagga aaaattgctg ttgagatatt tgattgatca
     4261 caagaaggag tatgtcacca gtcaacgttt agcttctgaa ttgttattat ctgatcggac
     4321 aatccgtaat tacttgcagc ggatcaaaga gttagtagaa aaaaacggag gtaagatcat
     4381 tgctaaacca ggttatggct accaactgca tattttacaa agactgacat ttgacttgtt
     4441 tttgagtcgg caagaaatag tacctggata tgctagagaa ccgcaaggat tttatgagtc
     4501 cgaagatcga caaaagtata tattgaacca attgcttttg gaagatgcca ttctttttat
     4561 ggatgatttg gcagaagagc tttatatcag tcgttccagt ttgacaaagg acatgcaaga
     4621 aatcaaggag cgattgattc catattcact aaaaatcgtt tctaagcatg gtcaagggac
     4681 atggatcgaa ggggaagaaa gaaatcgacg gcattttatc atggatactt tttttggaaa
     4741 ccattatgga aattcattga aagaatattt agggcaaagc cgttttttcc cagacatcag
     4801 ttttgaagag ctagtcatta tcattctcga tgaaacaaga gaagctaagt taaaagtttc
     4861 ggattttatc atacagaatc tgactttaca cttggctttg gggatcaaac ggctccgaga
     4921 agggtttgaa atcaaggaat taggcatcga acaagaagtt tctaagcgag tagagtatca
     4981 agtggcgcag cggatcgttc aacgtattga ggtaatcgca ggtgtatcgt ttcccgaaga
     5041 agaaattgct tatttgacat tacatctcat ggctaagtcg aatcacaaag aaaatcaaaa
     5101 tgatcaggaa ctatcaaagg aattagcgaa agttctgaaa agattgtctc aggaatttca
     5161 tttttcatta gtagaggatc tccagctttt caatggacta ctcgatcatt tgtcaccgat
     5221 gatgattcga ttgagccgag gtattcgcat tgagaaccca ttgactgagg agataaagaa
     5281 agaaaatccc aaagtctttg acgcagtaaa aaggcatttc agcaacatgc cggctttgaa
     5341 aaactatacc atcaatgaag atgaatgggc ctatttggct ttgcatctca tggcggcatt
     5401 ggaaaaagaa agagctgcgc ataaactaca tgcattgatt atctgtgcaa cgggttacgg
     5461 cagtgcccaa ctattaaaga atcgcgtagt cagtgaattc ggtaaaaata ttacagtggt
     5521 aagtgtaaaa ggctattatg agatcaatga aacaacttta aaaggaatcg acctgattat
     5581 ttcttctatt gatctttcga caatgttttt caaaattcca gttttacatg tcagtatttt
     5641 tttgaatgaa gatgatgttc gtaaaatacg aaaggtagtt ggagaatcta taccctatca
     5701 ttcctcagat agaccgatac cagcattttc tctacagcaa aaaaagcaga tttacaccga
     5761 acagttgtct gaaaaatttt tcaagactta tacgtacgaa ccgaaaaagg aagaaattct
     5821 tcatgatttg ctgcaaaagc tgagcataaa tgaagaagaa ggatatataa gagaaatgcg
     5881 aaagcagatc cagcaacgtg aaaatatggg acaaatcata ttcagtgata cagtggcagt
     5941 cccacatccc gctgttccag ttggaatcga aacaaaggta gccgttgcat tgattccttc
     6001 tgggatgcag tgggatcaat atcaagaaat tcattttgtt tttttagttt cgccttctta
     6061 tgtcgaaaat gaaggaatta cggtcgtgac taaagcaatc gttaggttag ttgaccagct
     6121 aaaagtacag caggaaatct tagctgaacc tatttttgag aatttcagca gtaaatttat
     6181 caaactgatc gaataaggag gaaaacagat ggctgaaaaa ataaacagtg aaacactaca
     6241 agtgacttct tttgatatca ttttccatag cggaaatgca cggacactga tacatgaagc
     6301 atttcgctcg atgcgtaaag agatgtttga agaagtagaa gagaagttgc gacaagcaaa
     6361 tgaagagatt cttcatgcgc atcagtcaca aactcaactt ctgaaagaat atgccagcgg
     6421 gacaaaaata gaaatggaaa ttatcatggt ccatgcacaa gatcatctga tgacgacgat
     6481 gacattattg gaagttgcac aggaaatgaa ttatttgtat caaaaaaata aatagaaagg
     6541 aaggatcatt gtgcaggata cagtaaaaag gaaaatcgaa ttagaaaaaa agcaacttag
     6601 tttgaaaaac atgtatttca atcgttatct aatcgtacgt tacctgactg cctttttctt
     6661 tttcatgaac atgcaatgga tgattctgct tctatcagct aaaagtttag gaagtagttt
     6721 accgatgatt ctgattttag ctattttgcc tgcagtagga gaacaagtaa agctttatcg
     6781 aaaacaccaa acgaacgttc catggacaaa gtggtatttt ttgttccaga gtgtctttaa
     6841 tttattagtg attccagtgt tgtttacctc aggatttaca ttcttgtatc cttttatggc
     6901 aaataacaat ggaggacagt tatttgtttt cattcttgtt gtaagcggta tttttgccag
     6961 tgcatttata cagtatcgtt taaagaaaat ttcactcaac caagaccaac agtatatccg
     7021 tattaaacaa tatgaaaaag cgttatattt aggaaaggag aataactaat gtttgatttt
     7081 ttgcagaaat acctgatggg acctatgggg aaaattgcgc aacttaaaat cgttcgggct
     7141 gtcatggcag cggggatggc ttctattcct ttcacgattg taggctcgat gtttttggtt
     7201 ttaaatattt tgccattacc gttcccattt ttagaaggat tttttaatgc aacttttttc
     7261 aaagttagtg acttgtacat gatcgtcaat acaatgacga tggggatttt atctatttac
     7321 tttgccattg tgtttgctta tgaattaacg tcaattgaac gagatgaaca aaaattaaat
     7381 gttaatccgc tcacgggggc attactatct gtttttgcat tctttatgtg tatccctgaa
     7441 ttgatcatga ctgatggtaa aatcagcttg attgcttcta tcaatgagac agaaactgta
     7501 gtaaatggta ttaggatggg ctcttttgtc gaacgtttag gaacttcagg aatctttaca
     7561 gctattatta tgtcgattat tgcggtcgaa ttgtattgta tgtgtgtcaa gagaaattgg
     7621 gtcataaaaa tgccagatgt tgttccgcca ggagtttctc gttctttcac tgctctgatc
     7681 ccaacatttg tcattgcgtt tgtggtgatg atcatcaatg gattattagt tgcactagga
     7741 acggatatct tcaaaatgat tgctatccca tttgggtttg ttacgcagtt gacgaataca
     7801 tggcttggtg tcatggtgat ttatttcttg atccatgcat tgtggatcgt agggatccat
     7861 ggagcgaata tcatcacttc tttcttgacc ccgatcgtat tagctaatat ggcagctaac
     7921 gcacaaggcg caaattatcc gttggcagga gaattcaaca attcttatgt aaccgttggt
     7981 ggatcaggtg cgactttagg attgatcatc tttattgcat tcctcgcgaa atctgatcag
     8041 ctgaaagtgt taggaaaagc ctcacttgta ccaggattat tcaatatcaa tgaaccaatc
     8101 attttcggga tgccaatcgt ctacaatccc tatttagcgg ttccgttttt cttagctccg
     8161 atggcttctg ctacactcgc ttattttgcc attcggctgg aaatcgtcaa accgatgctt
     8221 gcacagatgc cgtggccttc accagtcggg atcggagcat ttgttggtag tggtggtgat
     8281 tggaaagctg ctgtgttagc agtcatttgt gcaatcatcg cattcattat ctggttccca
     8341 tttatcaaat tctatgataa taaattatta aatgaagaaa gagaaaaggc agcagaatta
     8401 gctagtgaaa agttaaaatg atttctaaga aaaaaggaac tgtgacaaaa aatcacagtc
     8461 cctttttttc ttagaaatgg gaaaccacta gtatataaat accattattc ttcaatcaac
     8521 cgcttcacga ttgcataatc cccttcataa ggttcatcta caccattgat tttttgatag
     8581 ggatccacgc cttttcccgc aataacgact gaatcttgcg gagcgctttc tgctaaagcg
     8641 cgtcgaatcg cttcaggacg atccatttca taatgaacga tcaaatctgg attagtaatt
     8701 gcttcattga tttcttccgc gatttttttc ggatcttcaa atgctgggtc atctgccgtc
     8761 aaaaaggcga tgtcagctgt ttctgatagc acttttccaa aatcatgtcg acgggagatt
     8821 gctttgtttc ctgggctgcc taatacaaca attacacggc catctggatg ttcatttttg
     8881 gcaaatttca gcaatgtttt caagcttaga taattatgtg catagtccac atagacatgc
     8941 gctccattgg attggatcaa ttgatccatt cggcctggta cacgagcttc ttttaatccg
     9001 tcttgtgcat cttgactgga ggcacctgtt agtgctgcgg caatgatcgc actggctgca
     9061 ttttcttggt tgaatcctcc agccagcaaa atatcataag cagtatcgca gatagctaag
     9121 cgatcttcat gagaagataa agtaaaaccg tgagtacctt tatctgaacg gatcacttga
     9181 taattgcttt cttccgaacg accataagtg atcgttggaa tctggaatag atcggctgtt
     9241 tccctaagta aatcaaaata gtcactttca tgatttaaaa caactgtttt tgaattcaag
     9301 atcaattgtc gcttgcaata gaaatagtca tcaaatgtcg gatgttcgat cggactgata
     9361 tggtcaggcg agatattcaa gaagattccg acatctaaag tcaagccgta tacgcgttgt
     9421 gttttgtaag cttgtgaaga aacttccatc actagatgag tcataccatt ttcaactgct
     9481 tctgacatca tacgatataa atctaatgac tcaggcgttg tcaaatggga tttgaaatat
     9541 gttttgccat ctaacgttgt attcatagta gaaagcaatg ctgttttttt atttgttgtg
     9601 tgatccaaga ttgcttttgt aaagtacgca gcagttgttt taccttttgt tccagtgaat
     9661 ccgatcactt tcaatttatt ttgcggatag tcgtaaaaag ccatagcaag aagcgccatt
     9721 gctttacgga tatccgttac gatgattcct actgctgaat tttcgtatgg ttgttctgat
     9781 acataatatt gaataccctg ttcgattgct gaatccaaat attcttcttt gaaattcatt
     9841 cctttacaga aaaacaaagt agaagcatca gcttttcttg aatcataact taactgaaga
     9901 aattcagctt tttctggtac atcaagatgc cagcctcttg ttgaaacaaa ttcttttaat
     9961 agattttctt ttaatagtaa atgacggatg tcttctaaag agaagctcat tgattgatcc
    10021 ctcgctttat tcaatttctt ggtttattat agcacactct ttttaatata tcgtttgttt
    10081 ccgctatgac aaaatccagc tttattcaaa ttttcttgac acaggggaca agcggatgag
    10141 tattttcata aacaatgaac tgactgtaat agataggaga acgaatagtg tctccaacaa
    10201 tccaaaaaca taataattca aaaagacgat aagactattg gtaagtaaaa gtccttgcgg
    10261 cattggaagc cgaaaaaatt tgtggaaaat aatcggcggt atcgttatac cgccatttga
    10321 ggccccgact gtgtacaatg tgtaaacccc aagactgaac aaaagacttc ctgaaatcag
    10381 tgaaaagaaa tgtgaggaga tcatttccac ttttggaaca atctctaaaa caacaggaaa
    10441 aagcagactt cccgctacag tcttaaaaaa tactgatttt cctaatagga cagcggctag
    10501 aagcagcatc ataagattga tgctccaaac agtaactgag cgttggatag aaaaggccgt
    10561 ttcaactaaa tgtccgatgg cgcccactcc ggcggaagct atatcgtgag ggagcaagaa
    10621 ccaattcaat ccaactgtga taaagaaaag acctaaaaat ataacgggta gtttttttat
    10681 ttcttgtgtc attgatatct cctataaatg ttagccataa aaggctgtat atttctttac
    10741 tttaccaaaa aaacgaaacc gaattcaaaa aaacaattgt tcttataaaa gttatataaa
    10801 atcgattgcc agtttctgtt ttaccaatta aaatgatagt aatgttttgt agaaagttgg
    10861 aatctgaccg atggataatc aaagaaaacc agcattagat caattaacac cgcaagaaaa
    10921 aatggcacga ggctctgctt ggatgaccgc cagtaatatc atttcaagat tgctaggcgc
    10981 tgtctatatc attccctggt atgcttggat gggcgaaaat gctaaggctg ccaatggatt
    11041 attcaatatg ggatacaata tatatgcatt gtttctactg atctctacag caggaatacc
    11101 tgctgcgatt gcaaaacaaa ctgcacgcta taattcattg aatgaatatg gcacttcacg
    11161 acgattattt atccgtgcat tacagatgat gggcgggtta ggcctgttgt ttgctttgtt
    11221 catgtacatc gcctctcctt ggctagctca tgcttctggt ggcggagaag aactgattcc
    11281 aacgatgcgt tctttgagta tcgctgtatt ggtttttcct tgtatgagtg tgatacgagg
    11341 gtatttgcaa ggcaatcaag aaatgatgcc ttatgcgctg tcgcagattg tagaacaggt
    11401 ggcacgtatt ttttatatgc tattggctac attcatcatc atgaaagtca tggatggtga
    11461 ctatgtgacg gcggtcacgc aatcaacttt cgcggcattt atcggtatgc tagcaagtat
    11521 tcttgtcctt ttgtattttt tgaagaaaca tcaagcgtat acatcggcgt ttattcacta
    11581 cagcgaaaac aaaataaata ttgcaacgaa ggaattgttg ttagataccg ttaaagaagc
    11641 gattccgttt attattgtgg gttcaggggt aacgatcttc aaattagtcg atcagttcac
    11701 ttttatgaag atcatgtctg catccaccga gtattccaat gcacagttac tggatctttt
    11761 ttcgattttt agtgccaatc cagataagtt gacgatggta gttattgcat tggcaacatc
    11821 gatcgcgtct actgggttac cgttgattac agaagctgtt acgttgaaag atcgagtagg
    11881 tctggcaaaa ttgacaagcg gaaatttaca gttgttttca ttttttatgt ttccggcgac
    11941 atttggtgtt atgttacttg cctatccgct aaatacatta ttttatacac cggattctct
    12001 aggaagtaat gtactgatcc aagcaagttt tgtaggactc ttcctaggac tatatatgct
    12061 agtttccaac atgctgcaag gaatgtttga aaacaaggcg gcaattaatt atttggttat
    12121 tgggtttcta gtgaaactcc ttcttcaata cccagccatt cgtttatttg aagtatacgg
    12181 ccctttattg gcgacaatga tcggatttgc tgtgtcttgt accttgatct taaagagaat
    12241 ccatgaagta gcccgcttta aaccaagttt tgtctggcgc aggacattgc tgatctttat
    12301 tcttaccttg attatgcttt tggcttcgtg gatcacaaag atgatttttg gtgctttttt
    12361 aaacgaagat agcaaattcc agtctctatt gttgattctt gtagtagcag gtataggggc
    12421 tttagtgtat ggttatttag ctttgaaaat ccgcttggca gaaagactac taggaccagg
    12481 aatagcgcgt ttacgacata aattaaaaat aaaataagtg aatcagaaga gctagattga
    12541 tgctcttttg aaatggggga aacgcatgcg tttagataag tttttagcag atatgggact
    12601 tggcagccgc aaagaagtca aaggattaat caaaaaagga acgatcgaag tcaatggaca
    12661 agtggtgaaa agcgataaag cgcaagtaaa tgaattcgaa gatgcggtta cttatttagg
    12721 agaaatcatt acgtatcaaa aagattttta ctacattttg cataaacctg caggagtcat
    12781 ttctgctact caggataatc atgatcaaac cgtaatcgat ttattggaag accaggatta
    12841 tcgggaagat ctttttccag tcggccgatt agataaagac acagagggcc tcttgatttt
    12901 aacgaatgac ggggtgtttg cccatcagtt actatcacct aaaaaacatg tggagaaaga
    12961 atatctagct gaggttagcg gtataatgac agaagaagat atccgtttat ttgcagaagg
    13021 actgattatt gatggcgatg agcagacatt gccggcaaaa ctccaaattg atcaagtaga
    13081 tcaagagaca gaaacttcaa ttatccgtct gattttgcat gaaggaaagt ttcaccaagt
    13141 taagcgaatg gtcaaagcag taggaaaaga agtcttatat ttaaaaagaa tccgtatggg
    13201 aaaattttgg ctgcctaaag atttgaataa agggcaatat cgacccatga ccaaagaaga
    13261 gctagaacaa gtgagagaaa aataattgaa tggatagcta aaagaataag cagagtagtt
    13321 gtagcgccat ctgaaaagca gacttgtccg tctgactttc ggatggcttt tttgtgaaat
    13381 ttcggcttat ttccgtagaa gctacttctg gaacaccgtt tgttcggaat aagggactgt
    13441 gacaagactt ttgttacagt ccccattgtt ccttgtgact cagagttaga tagtaaggac
    13501 acgatctccc taatactcag gccaaaacgc tgaattcaca acctctgata aacggtgttc
    13561 aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttcccg
    13621 tttttttctc taatactcag gccaaaacgc tgatttcaca acctctccta tactttgttt
    13681 cattttccct atactttgtt tatttattct cattattgaa agcgctataa ttacgttgcg
    13741 aatgaatcga aaggggtttt tagatgaaaa aacgaaaggg gttcaatcat ttttttatga
    13801 atctatggcg ttataaagca cttgtcctga tggctgtacc gggtatgctt tggatgattt
    13861 ttttctttta catacctgta atggcgaatg tcgtagcatt caaaaatttt catatttcag
    13921 cagatgggtt cttggctagc ttgagggaaa gtccttgggt gggttgggaa aatttcaaat
    13981 ttttattttc ttcggatcaa gcatttctca tcactaaaaa tacaatttta tataatgtag
    14041 catttatcct tttgaatctg ctgatctcag tcgtatttgc aatcattatg agcgagttga
    14101 gaaataaacg actcgttaaa gtttatcaaa cgatgtcctt actaccttat tttctatctt
    14161 gggtcattat cagttacttt gtttacgctt ttttaagtcc tgacaaagga atattcaatc
    14221 agtggatcac tggacatgga ggagaagctg tcaattggta taatgaaccg aaatattggc
    14281 cgtttatctt ggtgttcatt ggtacatgga aagggattgg ttataacagc attatttact
    14341 ttgcatctgt tatgggaatc gatccgactt attatgaagc tgctatggtt gatggagcta
    14401 gcaaatggca gcagatcaaa catgtgacga taccacaact tattccacta atgacgattt
    14461 taacgatttt agccgtagga aacattttcc gagctgattt tggcctgttt tataacattc
    14521 ctaggaattc cggtgcatta tatgatgtga ctcaagtttt ggatacgtat atctacaatg
    14581 gcctgacatc cacaggagat tttggtatga cggctgctgc aggactttac caatcagttg
    14641 tcggctttgt cttgttgatg atcacaaata cgatcgcccg acgttttgat agcgattctg
    14701 cattattcta aggaggaaaa aaatgaaaaa aaagaaagtg gagagagtcc aagtccgctc
    14761 tttcaataag acggctaatt ttttctctaa tataatgatt gcactctttg ctatttcctg
    14821 tattttgcca tttatatttg ttatcgccat ctcttttacc gatgagtcca tattgtcgac
    14881 tcacgggtac agtttttggc cagaaaagtt cagtatattt ggttatacgt acttgttcga
    14941 ccagatgcag gataaaattt tccaagcgct tttcgtcact gtacttgtta ctgttttagg
    15001 tacactgatc aatagcacgg ctacttcttt atatgcttat gcgatttcac ggtcaaactt
    15061 tccattccgt cgttttttta ccgttttttg tttgattacg atgttgttct caccagggat
    15121 ggtggccaat tatcttgtta tgacaaatct tcttcagtta aaagatacga tttgggcatt
    15181 gatcttgcca atggctgtca gtcccttcaa tattattgtg atgcgcacat tctttaaacg
    15241 ttcggtctct gattcgatta ttgaatcggc acggatcgat ggagctagcg aactgcgaat
    15301 atttgttcag atcgttttac cgttagctgt tccaggtatt gcgacaatca gtttatttgc
    15361 cgcattgggt tactggaatg actggttcaa tgctttacta tacatccaag atgataagct
    15421 agttccacta cagtatcttt tgatgaaaat ccaaagtaat attcaatatc tgacacaaaa
    15481 ttcaggagca ggaagccaat tgacaggcgg attagcctca gttccaggag aatctgccag
    15541 aatggcgatc gtagtcatct cgacgttgcc tattgcgatc agctatccat ttttccagaa
    15601 gcattttgtc aaaggtctaa caatcggcgg agtaaaagaa taaagaaaca aaaatataaa
    15661 tcataaaatt aggaggaaga aaaatgaagt tatggaagaa actagccttt acaggtgttt
    15721 cagcgttgtt gttgggaaca ttagcagcat gcggaggttc tggttctaaa gaccaggcag
    15781 aagctagcaa ttcggacact ttgcagatgt atcaaatcgg agataaacca gataatttcg
    15841 atcaattgat ggaagtagca aacaaacgaa tcgaaaaaga gatcggtgta aaagtcaata
    15901 tcaattacat tggctggggc gattacgaaa agaaaatgaa cgtcatcatt tcttccgggg
    15961 aaaattatga tatcgccttt gcgaacaact atgtttccaa tgcccaaaaa ggagcctttg
    16021 ctgatttgac tgaattagcg cctaagtacg ctgaaaaagc ttataatgat ttagatgaag
    16081 catatattaa aggaaatctg gtcaatggaa aattatatgc tttcccagtc aatggaaatg
    16141 tctttgctca acaagtactt acttttaaca aagctttact agataaatac gatctttcaa
    16201 ttgatggaat ccaatcttac gctgatgcag aaaaagtctt gaaggaattc catgaaaaag
    16261 aaccaaacac ggcagctttt gccattggac aaggcttcaa agtccaaggg aactttgatt
    16321 atcctttagg gaatacgtta ccatttgcta ttgatctgga tggagatgat tcgaaaatca
    16381 tcaaccaata cgataatgaa acgtatatcg gactgttacg tacaatgcat agctggtaca
    16441 aacaaggact cattccaagt gacgctgcaa cgagcaataa ggattatcca ctagaaggaa
    16501 atacttggct aatgcgtgga gaaacacaag gaccgtatga ttatggcgat acaattttga
    16561 caaatgcagc aaaacaagaa cttgtctcaa aagcaattac tgttccacta aaatctacag
    16621 ctcaagcaca aatggcaaat tttgtcgtat caaatacttc aaaaaataaa gaaaaatcag
    16681 tagaacttct agggctgctt aacagtgatc cagaattatt gaatgggctt gtttggggga
    16741 tcgagggaga agcatgggaa aaagtcgatg gttcagatga taaaatcaaa ttgctagatg
    16801 gttatcagcc aaatacacat atgtctgctt ggaatacagg aaacaacaag attctatata
    16861 cacaagagtc tattacggat gacatgattg ccaaacgtga tcaatcgatt gctgatgcag
    16921 aaacttcacc gattttagga ttcagcttca acactgacag tgtcaaaaca gaattaagta
    16981 atatttctaa tgtcatgaat caatatttag atggattgaa tacagggact gttgatccag
    17041 atgaaacatt accgaaattg aaagacgctt tggacaaggc tggctatgat aaggtattga
    17101 aagaaatgca aaaacaatat gatgagttcc gacaagaata ataatgaata aagacaaagg
    17161 ggttgtgaca aaagacgtgt cacaacctct tgttccgaat aaacggtgtt caaaagccag
    17221 tttctcggta ttaagctaaa tatatgtaaa aatgtggaaa gccatattca caaaattttt
    17281 cttactactc gaaactaagc ggctatttca caaactcttg gtaaacggtg ttcaatcagc
    17341 taaccctcgg tattcgctca caaaacgaca aaatatgagg aactattttt ccgtttgttt
    17401 ctctaataat caggtcaagg tgctgattgc ccaacctttt tttgtataat gagaaagtat
    17461 aaacttaatg tttggaggag aagatatgtt agggaaggtt ttggaagagt tatttatccg
    17521 gatctgggtc attataaaac taaccttgta tttctggata tatacatttg ccggcgggat
    17581 catctttgga ttaggtgcgg catggaaaac agtcaatgaa ctattttatt tgtacggatt
    17641 cgagtataaa gaaatcacaa taaaaagagg ctggaatata tataaaagaa attttttaag
    17701 aggcaatctg ctatttagtt tatttttatc tggtactgct ttattgagtt acaatttata
    17761 tttatctgtt caaatcaaag ggctcatttt tttagctatt gattttattt tactttttgc
    17821 cttgctctgc ctatttgcta cttatcaata cagtatgatt ttagatagtg aatatacgat
    17881 ctctgttcct aatctgctaa agctaagttt tatttcggtt ttttccagct tttcgtcttt
    17941 tctaaaaata attattggta gcggtataat tttaggcgtg acttggcaat tcaaaggatt
    18001 gatcctgttt ggtgtcattg gtcttttgac ggtttggaat ggtacgatga caacacattg
    18061 gcgtgaagaa ttagataaac agttggaaag ttatgaataa gtttcggatt ctattttcaa
    18121 aaggaacctt actaaatcgt ctgttgcgga aatacgcggt tatcatgatc ggtgtaacga
    18181 tgactgctac cgttattttt agtatccata cttgggaaca aaatcaaaaa caggcagaaa
    18241 acatgacaag tgatgccgtt caaagcacaa gcagaatgct gaatgataaa acaaccttga
    18301 gtaggatcat caaaaaccag cttgtaggga attccgaaaa gatcgaaaat gtgacgacct
    18361 acttaacaaa acccatagat cagtatctga tgtatgtata cgaacagcaa aacagtaccg
    18421 atgaattagt ttctttccca aatcaaatca aagatctcta cgcaaattat gaagaactaa
    18481 gtgcgatata tatcgtgttg aatcaactac cggagtatta tgaatcttct agagaaaaca
    18541 aaggtgggga aataaaaaag gggacaccaa aattactaga cgccttttat atcaagttgc
    18601 ctatcacaca aggaggagct gagtcaggaa gtatttttat tggttttcaa aagaaagaac
    18661 tagatatgat tttagaaaat ctaacgtcat tcaatggact ctctttatac atgatttctg
    18721 gaacgacaaa tcgactatac acattccatg ataataaaat agctaaagaa acgtttgaaa
    18781 gacaagaaat gatcatcaca gagagtctga agaaaaattc acaattgccg attgacgatc
    18841 tggaaaaaaa caattttgtt caacaccaag aactgtctga tgattatcgt atattagctg
    18901 tactagataa aaaaagtgta aaaatcgaaa cgggaaaaaa tcttgccccg ctgatcattg
    18961 gtatcattat tttagacaat atactgctta tctttcttta taaaacattc aaacgatatt
    19021 cacagcaagt atctatcgtc atgcaggcca tggatcaaac agcagcaggg aatttagaga
    19081 cgcggatcga cataacaaac acggaatatg aactgaggga actttcaatc ggtatcaacg
    19141 aaatgttgga tagcatcaat cagtttgtag aagacatcta caaattagaa atcaaacagc
    19201 aagacgcaca tatgcgggcg cttcaagcac agatcaatcc acacttttta tataatacat
    19261 tagaatatat ccgaatgtat gcaatcagcg aaggaagtga agaattagca gatgttgtgt
    19321 atgcgttttc cgctttgctt cgaaacaata cgaatcaaga gaaaacgatc actttaaaag
    19381 aagaactgga cttttgcgaa aaatatgtct atttgtatca aatgcgttat cctaatcgag
    19441 tagcctatca ttttatgatc gatcctgatt tggaaaagat cgaagtacct aaatttgtga
    19501 tccagccatt agttgaaaat tattttaaac acggtattga ttttactcga ttcgataatg
    19561 cgctaagtgt caaagtcttg caggaaggca aacgggtaag gatcatcatc aaagataatg
    19621 gaaaagggat gactgaaaaa cggctaaaac agattgaaga aaaactgtct catcctaaag
    19681 tggaacttca cggttctatc ggtctgcaaa acgtgaatga acgtttacgt gccagcttcg
    19741 gcagcagtta ttatatgtcg cttgagaata atgagacagg tggactgacc gtttctatta
    19801 cattcaagga gggatgagcg tgtatcaagt gttattggta gatgatgaat acatgatttt
    19861 gaatgggtta aaaaagataa ttgattggca aagtttaggc tttcaaatag tagcaacagc
    19921 agagaatgcc aaagaaggac tagctgtact tgaacagcga cagattgatt tagtagtaac
    19981 ggatgtgaca atgccagaaa taaatggact ggaatttatt gaagcggctc aaaaagaacg
    20041 tcataatttt gaatttatga ttttatcagg ataccaagaa ttcgattatt tgaaaggagg
    20101 tatgcaatta ggcgccgtga attatttgat gaaacccgtg aataagttcg aattggttga
    20161 aagcctcaag aaaatcaaaa cacggttaga tcagcaaaat gaacaaaaaa atcaacaaga
    20221 aatctatcaa gaactgttat tcagtcaatg gttgaatgaa gaattggatg aaataagtga
    20281 agaagaaata ataggacaaa taagtgagaa gcagcatagg atagtattga ttcaactggc
    20341 aagaactcat gatactctta tcaatagatg gttgcaagaa cggcaacaaa tcttttacta
    20401 tcaacgaaat tacggagacc gtgtattgat cacgttgttg ttggaggcag atgaagttga
    20461 tggcttctgt tgttttgttc aagaaaactt tcctgatcag gaatggctga tcagtatcgg
    20521 ggaagagaca gattctttgg acaaaatccc tgaaacattc caacaagcga aagacaatct
    20581 acagttgcat caattttatg gagataaaga cgagcatgtt ttttacgcag tacaagcaga
    20641 aacaaaagat caaaccatcg atttttcaag tttcagacgc gtgctgcaaa ataaacaatt
    20701 ggaagtcgct caaaaaatga tcactgattt ttttgaacag tttcagttag cagccatgat
    20761 gccggaagat atacgatatt ccagtttttt actgtttatg gagattcaac gggaattgat
    20821 tgatctggaa gatgaggaat atttacaagg aatcgaaaaa atccatcagg caaaaacggt
    20881 gcaagaactt cagcagctac ttctgtcatt catcaaaaag catcagagac agaaaaagta
    20941 cagtgacaat gtggaaaaag taatcgtaat cctgcaccag cattatcaag aaccacttac
    21001 actcaaagaa gtgtcagaaa acctgcattt aaatgtcatg tatctaggac agctgttcaa
    21061 aaaagaaacg aaaaaaagtt tttcagctta tttgaaccat ttacgtatgg aaaaagcgaa
    21121 acaactgctt ttgcacagta atcaaaacat caatgagata gcaagtgaga ttggctacaa
    21181 caacaccacg tatttctcta aattattcaa gaaaatcgtc ggcagatccc ctaaagagta
    21241 ccgagaaaac agtggagaat cataaaagaa aaagccaatg taatgaaata acttataaaa
    21301 aattgtctag aaatctaatt ttgggctgtt cttattactt gaggcaaggc agctttttca
    21361 tgacctctcg ataaacggag ttcaaaagcc agtttctcgg tattaagcca aatttatgca
    21421 aaaatgtgga aagccatttt cacaaaattt ttcttaatac tcgaaactaa acagcttttt
    21481 cacgacctct ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaatg
    21541 gcaaaatatg aggaactatt ttgccatttg tctctctaat actcaggtca gaacgctgat
    21601 ttcacaacct ctcaataaac ggtgttcaaa agcttgcatc tgcggtaata agaccaacca
    21661 aacgaaaaat atgaggatct atttttgctt ggctgttctt attacttgat gcaggacagc
    21721 tttttcacaa cctcttgtta attatagttt gataaggaaa aaggcaaatc ttttgcttga
    21781 tatttcttag ggtgagggat gatccctaat tggtaagaga tcgtgccgcc tttcagcaaa
    21841 tcttgatggg taaaatataa tttcgtatat tctctgtcca tccgtttgat tttatggata
    21901 aattgttgct gcggccgatt aggcgttgct tcaatcgtca attcctttcc atttgacaaa
    21961 agcaaaacag ccttgtcaat taatggcata ccgataacat attcaccggt tccaggcgtg
    22021 acaggataaa atcccagact attgaagata taccaagcgg ccatacttcc attgtcctca
    22081 tctccagggt aacccattgg agaatcgtcg aaggtctgag tcatcaactg tttaagcagt
    22141 ggctgtgcca tttcaggttg tcctatataa ctgaacaagt aaggatagtg aaaacttggc
    22201 tggtttgaaa tagctaattg tccaaattct atcgcggcca tctcactcat ttcgtggatc
    22261 tcaaaaccat atcctcctgg gtcaaacgtt gggggctgat tgcacagctc aatgagtttt
    22321 tctttatatc tctcactgcc accatatttt ccgatcagtc ctgaaaaatc gtgataaaca
    22381 gcaaagctgc tttgccaagc acttccttct gcataatctt gtccccaacg attagctgaa
    22441 aatggttctc tgaacttgcc atctttatct tttgaacgca taaatcctga ctcatagtca
    22501 aagatatttt gatagttcaa tgattgctgc tgatatttct tcgtcatttc ctcttcttct
    22561 agtaccttgg caacttggct aatacaaaaa tcgctataag catagtctaa tgtgtgattg
    22621 actgattcgt ggtagtgatt aggaacatag ccatatgtca agtaatcttc tgttccttgg
    22681 cgtccataat ttggatgttt gctttgaacc gtagcacctt tttccattgc cgttagaaat
    22741 tcaggcatca agtctcgtcg gatatctttt acagcagcat ctgcaatgac cgcatcgatc
    22801 aatgtcccag gcataagtcc acgttcatca ggagacagcc attttggcaa atagccactt
    22861 tctctatagc tgttcaaaaa accttccagc atttcttcgt atttttctac agcaatcaaa
    22921 gaataaagag gataaaccgt tttatacgta tcccaaaacc cattatttgt atagagaaca
    22981 ccaggtttta tagatcgact agtcgtatca tagtggattt tttgcccact aggatcaatt
    23041 tcataaaatg tttgtggaaa taaaaacacc cgatataaat tatgataaaa cgtccgcaat
    23101 tgttgttgat catggtgtgt gagctgtata cgatcaaaat aggactgcca ttgttcttga
    23161 ctgtgagcaa ggtaagtctc tttgctttgg ttttgttcta gttccaggtt ataagctgct
    23221 tgttccgagc tgataaaaga tgtcccaaat cggatagttt gtttttttgt ctgttcaaaa
    23281 tacaacggga tcgagtcgct atttttgttg gaaacagcta actcatcaat tgttaaagga
    23341 tattcaaatc tcaatataaa ataaaaagaa aaatcaggat cttctgaacc agcaaaatgt
    23401 gtgatttttc cttccactgt gtaaggatct ataacaaaga gctgatggcg tcccggaaag
    23461 gataaaagta aaccgctgtc atttttgctg taatcaatag ataagattcc gccatacatg
    23521 ctaggtatca atgtagaaca gatgccataa cgtaattgac agatcgacag atgagtcggg
    23581 caaaatatac tttcttctgg gcggtaagaa ctttgagcat ggaaaagggt gttctcctgt
    23641 agttttccat tgatcggcgt aaacagcata tgactgaaat cacccatcca aggacttggt
    23701 tgatgggtaa gacggtagcc ttgaaaaacg cggtcatcag gatgaaacca ccagctgccg
    23761 ttttgatccg tagtttgcgg tgcaaaataa ttcatcccaa aaggaacgcc agtataaggc
    23821 tggcagttcc cattagagaa atttggctga ttcgctgttg catgtctagt atctatttga
    23881 ccgatattca ttttatttct cctttgttga ttgtcttttg atgaatgaaa ctggaaattt
    23941 actatcggat ggtcgatctt ttgtctctaa ccaagtgacc aaagcatctc ccgcaagctt
    24001 acctaactgt tcaaaatctt gtgcgatggt tgtcagtggc gggtcaacaa gagctgctgc
    24061 ttggatatca tcaaatccga taattgaaaa atcttcaggg atatgataac catgctgtct
    24121 caagagattc atggtctgga tagcgacaac atcgttttca caaacaaaag cagttacttc
    24181 atacttagtg atataagaaa gcaattcatc taatgtcgcg cttttgtcat ccagtgaagt
    24241 gtggaaagtc aaaccagctt tgtccaatgc ttcaaggtaa ccaaggtaac gctgtcgaac
    24301 ggattgtgga tgagtctgcg taccaaacaa ataagcgatt tttgaatgtc cctgttcgat
    24361 caaataagag gcagcaagcg caccgccttg gaaattatcg gaaagaacgg taggaaaagg
    24421 taactcatag atttttttat ctaatatgac gacagggaat tctttcaatg aaagctcaaa
    24481 aagtaagtca agatgttgtt ctgtatcaag agcataataa atcaatccgt caaattcatt
    24541 gattagctct tttgctgttt tattttgtag aaagtcaagc gtcgtcatca tgacatcaat
    24601 ctgtttatct tgcatcacag ggttcaatcc cttgctaaaa tcaccaacag acaaatcagc
    24661 gacatatgga agaagaaaca gaatccgtcg tgtttttgta ggagaagcaa gaatctgttc
    24721 cggagaagcg gatttaacga aactgccttt cccgcgaacg cgataaataa gtcctaattg
    24781 ctctaattca gtcaatgcac gtttggaagt gattctgctt actttatagg tacgtgaaag
    24841 ttctttttca gttggtagtt ggctgtctac aggtaattca ccagttaaaa tagccgtttg
    24901 caaatcatta aatattgtct ggtacaatgg ttgagtcata taaacacctc ttaatgatat
    24961 atcatattca aataatacca taatggatga aaaagtaaat aaaaagatat tttttgaaag
    25021 cgtagacata attggctgta tgaagtataa tctttaaaga tatatcaaat agaaagaagg
    25081 ttttattagt ggtttataaa gatataccaa aatcggtgca gaaatttatg gaagatatca
    25141 cacaaaaatg tggggatatc catgcggact gggcaaagaa ttttacagca gcatttgcca
    25201 atactctgtt gacaacagtc aaaaagcaaa atgacggaac caccttttta ttgacgggag
    25261 acattccagc gatgtggtta agagactcta ctgcacaagt tcgtccttat ttagtgattg
    25321 caaaagaaga tgaggaatta gctgacatga tcagcgggtt agtcaaaaag caattttttt
    25381 atatcaatat cgatccatat gcgaatgcct tcaacgaaga agctaacggt gctggccacc
    25441 aaacagatca tacaaaaatg aacgattgga tctgggaaag aaaatatgaa attgattctt
    25501 tatgctaccc aatacagttg gcctatcttc tttacaaaaa tacaggaaaa actgatcaat
    25561 tcaatcaatc ttttattgaa ggtatcaaaa aaatcttgga cgtgttcaaa acagagcaag
    25621 atcatgaaca ctctccttat acatttgtac gagacacgac aagaaaagaa gatacgctga
    25681 tcaatgacgg gcgaggtgca aaaacagctt ttacaggaat gacatggtct ggtttccgtc
    25741 ctagcgatga tgcttgccgt tatggatatc tggtaccatc caatatgttt gcggtggtcg
    25801 tgttaggtta tgtggaagaa atttttcaaa cgatactcga tgataaagaa attgtacagc
    25861 aaacacagaa attgaagaaa gagatccaag aggggattca gaatcacgga cgaacggtta
    25921 atcaaaaagg gcaaacgatt tatgcatatg aagtagatgg aatgggaaac gccacagtca
    25981 tggatgacag taatgtgccc aatctgatcg ccgcacctta cttgggatat tgttctgaac
    26041 aagaggaaga ttatctgcaa acaagaagaa cattgttgag taaagaaaat ccatatttct
    26101 atgaaggaaa gtacgcaaaa ggaattggat cctcccatac gccagaaaaa tacgtttggc
    26161 cgattgcatt agctatggaa ggaatgacaa cgtcagacaa atctgaaaaa gagcggatat
    26221 tgaatttact tgtagcaact gacgcaggaa cacaccttat gcacgaagga ttcgatgtga
    26281 acaatcctga aaattatacg agagaatggt tttcttgggc aaatatgatg ttttgcgaat
    26341 tagtcatgga ttactttgat attagagtag aaaagtagga ggtatcgtaa atgaaaaaga
    26401 aagtatatat catttctcat agccactggg atcgtgaatg gtacatggct tacgaacagc
    26461 atcacatgcg actgattgaa ttgatagacg atctactaga gctatttgaa gttgatccgt
    26521 ccttcaacag ttttcatttg gatgggcaaa cgattatttt agatgactat cttcaagtaa
    26581 ggccagaaaa aagacaggca atccaacaag caatcaatga aggaaaattg agaattggac
    26641 cattttatat cttgcaagat gattttctta tcagctcgga atcgaacgtg agaaatatgc
    26701 tgattggcat ggaagaaagc cgtaaatggg gaacccctgt catgttaggc tattttcccg
    26761 atacatttgg aaatatggga cagacgcctc aattaatgaa acaagctggt atttcagcag
    26821 cagcgtttgg ccgtggtgta aaaccaattg gctttgataa tcaagttttg gaagcagaaa
    26881 actattcttc ccaatactca gagatgtggt ggaaaggccc tgatcaaaca gcaatctttg
    26941 gtctgctatt tgccaattgg tatagcaacg ggaatgagat tccagtagaa aaagaagcag
    27001 ctcttgcctt ttggaagcaa aaattagcgg atgcagaaca atatgcctct acgaaccact
    27061 tattgatgat gaatggtgtt gatcatcaac cggtacaaaa agatatatca aaagcaatcc
    27121 atctcgcaaa tgaactgttt ccagattacg agtttattca ttctaatttt actgactatc
    27181 tggaagcagt tcaaaaagat gtgccagaag acttaggctc agtagaagga gaattgacaa
    27241 gtcaagaaac ggatggctgg tatactttag ccaatacagc atctgctcgg gtttacttaa
    27301 aacaatggaa taccaaggtc cagcgacagc tagaaaatat tacagaacca cttgcgacga
    27361 tggcttatga agttagtgga aactatcccc atgatcaatt agattatgct tggaagacat
    27421 taatgcaaaa tcatcctcac gacagtattt gcggttgttc agtcgattct gtccatcggg
    27481 aaatgatacc tcgttttgaa aaagcagatg aagttggaaa gtatcttgca caagattcct
    27541 tagaacagct gaccgcagca atcgatacga ccggattccc aaaagacagc tttccatttg
    27601 tcattgtaaa tactgcagga atggataaaa ctggtgaagc agaaataacc atcgaattag
    27661 aaagaaaaag attcgctgaa ggaatacctg aacaactata tcaagagtta gaaaatttgc
    27721 ctaaacgtaa gtatcatgta gaaacaaaaa gtggggccac tataccagct attttatctg
    27781 aagaaactgt ccaatttggt tatgatttgc cgaaagaccg tttccgtgtg ccatatatgg
    27841 caagaatgat caaagtgact ttaccattag aaaacatgcc agcattttct tgggaaacat
    27901 tcgcgcttgt agaaggggaa gcgaaagcag aagaaaagga aacaatgatt catcagagtg
    27961 gacgaatcat cgaaaacggc cctctacatt tgacgattga aaaaaatggt accattacaa
    28021 tggaagatcg caaaaacaaa cgaaagttga ataatctgca tatttttgaa gatatcggcg
    28081 atataggaaa tgagtatatc tttaaacaac ctttctgtga tcagccaatc ctatccagta
    28141 acaaagaaaa ctctgaagta aaagtattag tagatacacc ggaaatagca aaaataagta
    28201 ttctacagga aatggaaatc cctgtttctg cagacgagcg tttagaaaaa gaacagcaga
    28261 tggttgtgga atttcgttat cgaaaagcag aacgatccaa agaaaaacga atccttcaaa
    28321 taaaaacaat catgacaatc agaaaagata gtaaaaaaat cgactttgaa acgactctcg
    28381 acaatcagat gaaagatcat cgcttacgtg tcttgtttcc tacaaagtta catgtggaac
    28441 atcacgaagc agatagtatt tttgaagtgg tgaagcgccc aaaccatgtg tctaaaagtt
    28501 gggaaaaccc cacgaatccg caacatcagc aagcatttgt caatattcat gatgaagagt
    28561 acggtgtaac agtcggaaac tttggtttga atgaatatga agtaacagaa gacggacaaa
    28621 tcgcagtgac gctacttcgc agtgtgggag aactaggcga ttggggatat tttccaacac
    28681 cagaagcaca gtgtctaggt gagcatcggt tcaattacag cattgaacta catggaccag
    28741 aagaaaaatt ctctacttat ttgcatgcat acgcagcaca gattcctttt tccactcaac
    28801 aaatcaaaca tcatgaagga acattgattt caaaacaaca atatttaacg ataaaaagtg
    28861 aaacgtttgc aattacagct ttgaaacgca gcaagtttag tgataaagta gtagtaagag
    28921 gtttcaatat gtctagtcat ttagaaaaat tggaaataac taaagacaat ggaaaaactg
    28981 tcatattgaa tctgcttgaa gaaccaacga agcaagcggt tgtcccaata atccaacctt
    29041 atgagattcg gacaatcgga ttcgaggagg aaaacgaatg tatggcggaa tagaagcagg
    29101 cggaacaaaa tttgtgtgtg cggtttcaga tggtggagaa attttagaaa aattaagtat
    29161 ccctaccaca acaccagaag aaacattgga tcaggtattt gaattttttg atcgttatga
    29221 attgaaggga atcgggattg gctcattcgg cccgattggt atcgacaaaa caaaagataa
    29281 ttacggatac gttttggcaa ctcccaaaaa agggtgggca caattcgatt ttcttggcac
    29341 aatcaaggaa cgctacaata ttcctgctgc ttggacaacg gatgtcaatg ctgctgcata
    29401 tggggaattg aagcaaggag cagcacaagg aaaaaatagt tgtatttacc ttaccgtggg
    29461 aacaggaatc ggtgcaggtg tcgtgatcaa tgaagaaatt ttttcaggga tcgcacatcc
    29521 tgaaatggga catatttggg tgaaacgcca tccagatgac cattatgaag gtacttgtcc
    29581 ttatcataaa gattgtttag aaggattagc agcaggaccg tctattgagg cacgcacagg
    29641 gatcaaaggg caagatctgc cgcaagatca tcctgtttgg gctattcagg cttattatat
    29701 tgctcaagca ctgatcaact atacgttgac gttagcacct gaaaagatta ttttaggcgg
    29761 tggggtaatg aatcaggatc atctccttca aaaaatccgt cagcaatttg ttgaattgat
    29821 gggtggttat atggaaacac ctcaagtaag tgaatatatc gttcgttggg gactgccgaa
    29881 tgaaagtggg attatcggta gtttgctgtt agcagaacac gaacatcaaa agtaagcaga
    29941 caaattacac aaaaagaaca gagtcttatc attatctgct gataagactc tgttcttttt
    30001 tatttctatt cccataatcc aggtaaagct ttaccgacta ttttttcttg agtaaatgga
    30061 tggatcatgt gcagttctaa tgcatggagc atcagtcgtt cagctgttct attttggtag
    30121 agagggtctc caacgatcgg atgtccctta tgagctaaat gaactctgat ctggtgtgta
    30181 cgtccggttt ccaaaacaca atagacttgt gtttgttcag aaagtacttt gttgacagtg
    30241 acatgtgtca aggcgctctg accattttta tcatctacga ccctttttct ccgatcatgg
    30301 cgatctcgtc caattttatt ggtgatggtc atatctttgg caatttctcc ccatacgatg
    30361 gcttgatatc tccgatagat cattttttgt tccaacattc ttccaagtat cggcaagaca
    30421 aatggatttt ttgcaaataa aacggcaccg cttgtttctt tgtccaagcg gtggacaacg
    30481 taaggtcgtt gctgtttagt gtcgagataa gcagctaaat gattgagtaa ggtactgttt
    30541 tctgtcggtt catttggatg agtcttcatt cctgcaggct tatccactat aatcaaatgc
    30601 tcatcttcat ataaaacacg gatgtgcttc gcatctccta attgaacttc ttgataatta
    30661 taatctgttt cttcgaataa caatgtgatc tggtcattgc cctttacttc atgatggaac
    30721 aaagcaggtt gttgattgat ccaaacgttt ttacgtatac gcaaaaagtg tcgaaccttt
    30781 ctaggaacta accattcctt ttcaagcaag tcgcgaattg ttgtttcagg atggttcata
    30841 ggtaaagtga tcgtgatttt cacaagtgta ctcctttcag tgcaattgca gactaattgt
    30901 accattttta aaacatattt aagaaaaaac ttacattttt ctgttcattc ttatgttact
    30961 ctacttgcac taacgtaaaa aaacatattg aactacaact taagcaggaa gaaaacactc
    31021 tttcactatg gtaaaataag acgattgaat caaatagaca ggagaagaag caaatggatt
    31081 ttcaaagcat tttagaaaag ataaaagctg ctttgatcag tttttggcgt tggatcagac
    31141 catatctcgg acaattccat cgatggcgaa agcgcatctg gaaaaaatat catgtgaaca
    31201 aattgatttt gctgattggt ttgatctgtg tcttagtgac aagtatctac ctctttattt
    31261 tagcaaaaca agcaaatgtc gaaacgttaa aatcaggatt aagccaatcg acagtcattt
    31321 atgataagaa aaacgaagaa gctggcacac tgtacgccca aaaaggaaca tatgtagaat
    31381 taaacgcgat ttctccctat atccaagatg cagtaatctc gacagaagat aggaattttt
    31441 acgagcatca cgggtttgat atcaaaggga ttgcccgtgc agcagtccga atggtcttgt
    31501 ctggcggaac atcagggggc ggaggaagta cgatcactca gcagcttgcc aaaaatgcgt
    31561 atctgacatt agaccaaacg tttgatcgaa aagcgaagga attatttttg gcaatcgaga
    31621 tcgaaaagaa gtacagcaaa gaagaaatct tgaccatgta tcttaacaat gcctattttg
    31681 gcaacggggt atggggtgtc caagacgctt ccagaaagta ttttggagta gatgcaacga
    31741 atgttacgct gtctgaggct gctactttgg caggaatgct gaaaggtcct ggtatttata
    31801 atccgatcga tcacccaaaa aatgcgaatg atcggaaaaa cacagtacta agtgtaatgg
    31861 aagaaaacgg aaaaatcaca aaagaacaag aacaagcaga aagccagaca gatatcagcg
    31921 cgtatcttaa tgacacatac gataattcag attctggtta tcgttatcct tattattttg
    31981 atgcagtgat cgatgaagca gttaacgagt atggtcttga tgaagaagat gtcatgaata
    32041 aaggctataa aatctataca gctttagatc aggattatca aaagcaatta gaagctactt
    32101 atcaaaacag tgcattattc ccagcaaatg cttcagatgg tgccatggtc caatctgctt
    32161 cagtagcttt agatccgaat acaggtggtg tacaagcact agtaggacga agaggagatc
    32221 atgtctttag aggattcagc tttgctacac agatgcgtcg ttcaccagga tcaacgatca
    32281 aaccattaag cgtatataca ccagcactgg aagctggata caagccttcc agtatcttga
    32341 aagacgaacc gcaaagttat tacgatgcgc ataattttga cgggacatac caaggagaag
    32401 taccgatgta tcaagctgtt gcacaaagtt tgaatttgcc tgctgtatgg cttttgcatg
    32461 agatcggttt acaaaaagga tatgataaag cagaagaatt cggcctgcct ttagcaaaat
    32521 ctgataagta ttatggctta gcccttggtg gactggaaaa aggagtatct ccattgatca
    32581 tggcgggtgc ttacagtgca tttgccaatg atggtcagat gtatacgccg catcttatca
    32641 cgaaaatcgt tgattcgact ggagcggtga tcgtcgataa cacaaacaaa aaaccaaaac
    32701 aggtcatatc aaaggaaaca gccgatgaaa tgaccagcat gcttcttggt acattttcaa
    32761 atggaacagc ggctgctgcg aatcctgcag gttatacgat agcagggaaa acaggaacca
    32821 cagaaactaa tttcgatgcc acaaaagcaa atgaccaatg gatgatcggt tacacgccag
    32881 acgtagtgat ttcgacatgg atgggttacg aagaatccag caagctgcac tatttggaag
    32941 ggacaagtgg aaatgttgtc gggaaagtct tcaaatcagc ggcagaaggc atccttccgt
    33001 atacttcaaa aacaggattt agtgtagcag atgcctatgc cacaggagga caagtggtag
    33061 ctgcagatca agttggaaac acaggaaatt ctaatggaga gtctggaaat tggcaagata
    33121 acttgaatca atatggtgaa caagcaaaag aagggctgaa aaacttcggc gatatggtga
    33181 aagaaggcgt tcagggaatt ggtgaagcgg ctaaagacct ctggcggaaa tatcaaggag
    33241 agtagcccct ttgaaaacga ataaccccat gttacaatag agagtaacta ctaaaagaaa
    33301 agagggcata acatgtcaaa catctacgat agcgcgaatc aaatcgaacg tgagatccgt
    33361 gaattaccag aattcaaagc gttgaaagaa gcgtacgaga aagtacaagc aaacgaagaa
    33421 gcacacaaac tgttcaaaga cttccaagcg atgcaaatcg aattacaaga aaaacaaatg
    33481 agtggacaag agttcagcga cgaagatgca atgaaagcac aagaaatggc tgtgaaaatc
    33541 caatcagaag aagtaatcaa tgacttgatg caaaaagagc aaggcttcag tacaatcatc
    33601 aatgacttga accgtatcat catgacacca gttcgcgacc tttacagcga ataaaactat
    33661 tttatgaaac aacagagggt gtgaaagaga cgttcggcct tgagcgatga caaaattgct
    33721 tcttattgcc gataggctgt ctcttgaaca ccatttacca agaggtcgtg agcctaccgt
    33781 ttaactctga gttacaagga acaatttcaa aaaacagctt ctcatgtttg atgactattg
    33841 tgacttgtga ccgaggagtt ggcaggagaa caccgtttat tcagaaatag gagctacgac
    33901 aaaagtcttg tcatagctcc tattttttgt cattcatttt tcaagcggta gaaatagcct
    33961 ttttgagcta acagtttttc gtaagtacct atttcaacga tttttccttc atcgatcaca
    34021 acgattttat ctgcctcttt gatcgttgaa agccgatgag ccacaataaa agtagtgggt
    34081 gtttctgtca ggtaattcag ggcttgctgg atttttttct ctgactggtt atctaaagct
    34141 gaggttgctt catctagcaa aatgattttt ggctgacgta tcaacgctcg tgcaatggag
    34201 atacattgct tttggccacc agacagcttg ttgccatgtt cgcctactaa ggtatccaga
    34261 ccatcaggaa gctggtcaat cagtgaagaa agttgcgcag cttcgatgac ttcatctaaa
    34321 cgttcttttg atacgttttt caagccataa gtgatgttct ctttgatcga agcggagaac
    34381 aacaaagtgg tttgaggaac aacggctaaa taatttcgca cacttcgcat atccagttca
    34441 tccaaaggct gatcatctag aaaaattttt cctgaagtcg gctggataaa tccaatcaat
    34501 aagttaagga gtgtagattt tccagagcca gatggcccaa caaaagcaat tgtttctcca
    34561 gctttcacct ctagagaaaa atgatcgata atcggtttga gtgagtccgg ataaagaaaa
    34621 gaaacatcct caaaacgaaa atttccatcc aaatgttgga tttttttctt accgtgataa
    34681 gtttctacat cgccagcaat caacagttcg ccaatagaag tgatagattc catgcctttt
    34741 gaaatcgtag gaatcagcgt gatcattcca gaaacggaat tgacgatcgt cgtgaaatac
    34801 gtctgataca tcacgacatc gccgggttgg atgcgccctt gtaaagctaa tatcccagtg
    34861 aaaatcaaac aaatcagctg aaatatctga aaactagccc agctgactga tccgaaaaca
    34921 gattgcagga tatctaagtg gaatcctttt tcatagattt gagtaaaatg cttatttaag
    34981 cgagttgttt cgtgtttttc taacccatga gctcgagcaa tcggtgctaa ttcgatcact
    35041 tcgatcactt ttgcagaagt ctcttccatt tcttgcctga agtctgagtt ggactgtctg
    35101 atccttttac ggaaaaagag agtaacgaat gcggcgagag gaacagtcaa taagaaaaac
    35161 atgaaaacaa caggactttt ataaagcgtg atacctaaag ctacgaataa attgacagcg
    35221 atatttaatc cgctgacaaa tacttgagag gacaacgttt ggatagcttc tacatcacgg
    35281 atgatctttg actggattcg tccagattgc aattcttttt gataaggaat agatagttct
    35341 tggattttct tgataagggt cgctcttagt ccagcttcta cttgacggat cgaagtgcta
    35401 tggtatttta catgaaaata attcgtcaat atattttgac caatcagaca agccatcaaa
    35461 aaagcgttga aataaatggc ctgcaactga gaagaatctt tcgatgaagc tgcattgatt
    35521 acattggcaa tagcgatcgg cattaaccaa gatggaagat gtttgataat aaaaaagagt
    35581 cctgacatgg caaaacgcca ataatttccc tgataaagat gcagcaatgt ctgaaaagca
    35641 tttgcatgat atttttgata acttttctgt attcgttcct ccataatctt ctccttaaca
    35701 atcttggtaa ataaactgca agtgttctat tgcttcagga acataaatct caggagtagc
    35761 ttcaagagtg gcgtacatta gaggacgctc atatttgata taatgcagta ccgggcggaa
    35821 atctaaacag cccttgccaa caggtaccat gcggatcttt ccattttcca tgatatagtc
    35881 tttgagatga agcgctgtaa tataaccatg taaattgtcc aaggcacctc ggatcacttt
    35941 ttcctgatca gcatgatttt ctttatgcat caaattcgca caatctaaaa tgatttttaa
    36001 atttggtgaa gctacttcat cgattaaacg tttagctaac tgatagttat agattggatg
    36061 attgatccct gcttcaacag caacagttac gccaaaattt tctgcatgag cgactaattc
    36121 aatgatagaa tttctggcga tctgataagc ttcttcagta aaattttctt ttgtgtaacc
    36181 gacagagaca ctaccggttt ccgtagcaat catcttggct tgataggcag agcacagagc
    36241 aatttgctgt ttgaaagttg ccaatgcttg tgcacgaact tcttgatttt tgctggatag
    36301 gttgatatag catcctaaaa tggaaagttc gatattgttt ttttggaagt attcaccaaa
    36361 ataggcagca ctcccaagtg tcatgtcagt taatttaagt tgttcaggaa atgctttttg
    36421 tggtgcaaat tggatatggg acaagtgaag ctgtgatact ttttgaacga tttcttcacg
    36481 atttcgaaca tctaaatcgt gggcacggat gcctaaattt aatggaaaca tctcaaactc
    36541 ctttttgtaa ataattagca agaggcagac gggattcttt tatcaattca acaattattc
    36601 gggcaacttt atcagcgcct tctggactaa agtgggtatt gtcttttagt ccttctgggt
    36661 aatttttaca agtatcaggc ataagatgca ggtagaattt ttttgtctct tcttttgaga
    36721 aggtctggaa caattcttgt gatcttgaga acacatctaa aagaggaata tggtgtttct
    36781 cagcaaatgc acgcatcgct tttggatagt ctccaagtat atctggattc aatgttccgt
    36841 tttctaagta atctcgtcgc gtcacagatg tcagtaagac catttggacg ttcgcttttt
    36901 ttgcgcattc aaaaaatatc tgcagatttt ttaaatagct accgaaggga agcgtacctc
    36961 tttcttcttg gattttctgg tcgttatgtc caaattgtac aaataagaaa tcattcggct
    37021 gaaactgtga agctagacgg gctaaccttc cttcctggat aaatgatttg gtgctgcgtc
    37081 cattctttgc ttggttattg atctggatct cgggtgaaaa ataagcagag attttttctc
    37141 cccaacctgt ctcgggacgc ttgttttcat ctttttctgc cgcagtagaa tcaccggcaa
    37201 tatggatagt aggcatgtta tctcttcttt cataaaggtt tataggcaat gaagcagaac
    37261 agcaagagaa gaagacggag caa
//
LOCUS       DAPCMT010000013        34998 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPCMT010000013 DAPCMT010000000
VERSION     DAPCMT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.013"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            577..735
                     /locus_tag="RD743_000528"
     CDS             577..735
                     /locus_tag="RD743_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0562386.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            807..2033
                     /locus_tag="RD743_000529"
     CDS             807..2033
                     /locus_tag="RD743_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0562387.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(2121..2513)
                     /gene="rpsI"
                     /locus_tag="RD743_000530"
     CDS             complement(2121..2513)
                     /gene="rpsI"
                     /locus_tag="RD743_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDU0562388.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2529..2972)
                     /gene="rplM"
                     /locus_tag="RD743_000531"
     CDS             complement(2529..2972)
                     /gene="rplM"
                     /locus_tag="RD743_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDU0562389.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3491..3958)
                     /locus_tag="RD743_000532"
     CDS             complement(3491..3958)
                     /locus_tag="RD743_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDU0562390.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4078..5616)
                     /locus_tag="RD743_000533"
     CDS             complement(4078..5616)
                     /locus_tag="RD743_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDU0562391.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5696..6382
                     /locus_tag="RD743_000534"
     CDS             5696..6382
                     /locus_tag="RD743_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDU0562392.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6583..7110)
                     /gene="msrA"
                     /locus_tag="RD743_000535"
     CDS             complement(6583..7110)
                     /gene="msrA"
                     /locus_tag="RD743_000535"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDU0562393.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7194..7871)
                     /gene="pgmB"
                     /locus_tag="RD743_000536"
     CDS             complement(7194..7871)
                     /gene="pgmB"
                     /locus_tag="RD743_000536"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDU0562394.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7868..8998)
                     /locus_tag="RD743_000537"
     CDS             complement(7868..8998)
                     /locus_tag="RD743_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDU0562395.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8998..11181)
                     /locus_tag="RD743_000538"
     CDS             complement(8998..11181)
                     /locus_tag="RD743_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDU0562396.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11178..12203)
                     /locus_tag="RD743_000539"
     CDS             complement(11178..12203)
                     /locus_tag="RD743_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0562397.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12248..13075)
                     /locus_tag="RD743_000540"
     CDS             complement(12248..13075)
                     /locus_tag="RD743_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDU0562398.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13072..14139)
                     /locus_tag="RD743_000541"
     CDS             complement(13072..14139)
                     /locus_tag="RD743_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDU0562399.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14165..15001)
                     /locus_tag="RD743_000542"
     CDS             complement(14165..15001)
                     /locus_tag="RD743_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0562400.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14998..15867)
                     /locus_tag="RD743_000543"
     CDS             complement(14998..15867)
                     /locus_tag="RD743_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0562401.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15937..17220)
                     /locus_tag="RD743_000544"
     CDS             complement(15937..17220)
                     /locus_tag="RD743_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDU0562402.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17234..18919)
                     /locus_tag="RD743_000545"
     CDS             complement(17234..18919)
                     /locus_tag="RD743_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDU0562403.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19194..20219
                     /locus_tag="RD743_000546"
     CDS             19194..20219
                     /locus_tag="RD743_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0562404.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20814..24488)
                     /gene="rpoC"
                     /locus_tag="RD743_000547"
     CDS             complement(20814..24488)
                     /gene="rpoC"
                     /locus_tag="RD743_000547"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDU0562405.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(24546..28163)
                     /gene="rpoB"
                     /locus_tag="RD743_000548"
     CDS             complement(24546..28163)
                     /gene="rpoB"
                     /locus_tag="RD743_000548"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDU0562406.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28891..29859
                     /locus_tag="RD743_000549"
     CDS             28891..29859
                     /locus_tag="RD743_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0562407.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29877..30782
                     /locus_tag="RD743_000550"
     CDS             29877..30782
                     /locus_tag="RD743_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562408.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30779..31585
                     /locus_tag="RD743_000551"
     CDS             30779..31585
                     /locus_tag="RD743_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0562409.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMAILQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31721..32710
                     /locus_tag="RD743_000552"
     CDS             31721..32710
                     /locus_tag="RD743_000552"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDU0562410.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33027..33551)
                     /locus_tag="RD743_000553"
     CDS             complement(33027..33551)
                     /locus_tag="RD743_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDU0562411.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33838..>34998
                     /gene="scm"
                     /locus_tag="RD743_000554"
     CDS             33838..>34998
                     /gene="scm"
                     /locus_tag="RD743_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDU0562412.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTS"
BASE COUNT        10053 a         7393 c         6535 g        11017 t
ORIGIN      
        1 tgacaaaact atttgaaact acgcgtatag taagtagctt tcaaaatata taaaaaagga
       61 gcgttctcaa atgagaacat gctcctaaaa ctactttatt ggcgtatgga aaaattatac
      121 ttgaagtgga tagcgggttg aataagttga taagcatcaa aacatttata aaaattattc
      181 aagactatgt ctgttccata tacaaatatg aaaatcagta aactccattt aacttttttc
      241 gtagtgtgtc tttttataca tgtttatcgt tattaatagt gaataggaaa aaaactattt
      301 ttgatcattg acaattaaat acaatttttg aataacgatc cctgctattc gagctagaaa
      361 ctcaagtacg ccatgacctg attagcgaca aatacttaga gatttctttt tgttctggga
      421 aaacaaaatg ccatgacaat agcagcaaat aatgatactt ccgtaaggct cgccgtaaac
      481 tggggagagg tgaaattcct atgtgcttac ccaaagcaat tcaaaaattt cataactact
      541 attttaggag atgattttat agaatccaga gtctacctga ataaaaaaca aacctgtgca
      601 tttctaggaa tcgataataa tactctagat aagtggattc gtatatataa tttcccagta
      661 ataagagttg gtggtgtagt tcgctttgac gtagaagata ttaaagactt tatgaataca
      721 tttaaaataa cttgaaattt tcttctcgcc gcatatacta aaattagtga aatatactgc
      781 cttacttttt ttaaggagtg aggattttgc ctacaaagct ttcaaatggg aaatataaaa
      841 ctaatctgag atatccaaaa aggtttaaag aaataacagg aattgcatca gaaaagtatc
      901 aaaaaacatt tccaaatagg caattggcga ttaaagccga aaatgatatg aagaagaaaa
      961 tagaaaaagt tttgagagag gagaatgcaa actcattaga attaaaagga aaaataacgt
     1021 ttaaaaaatt ttacgaatct aaatggcttc ctagatatga attaggtcaa acaattagat
     1081 caaacaggcc tcctagtgat ataaccatta gtaatacaaa agatatattt cggttgcata
     1141 ttttacctat gtttggtgaa tacgcaatga attatctaaa tatcaatact gagataatat
     1201 cagatgaatt aaccaaaaaa tcgaaagaat atgcaaatat aaaaatcata aaaggatatg
     1261 ttcgttccat gttcgatatt gcagaaatac taaattatat cgaatttaat cgaactacta
     1321 aaattattca aagtatcact gcacctaaaa aaaatgcttt agaagaaaaa agaatacaag
     1381 aaggtaagca agcactatca tcaaaagaac ttactaattg gatagaagca gtaaatgatg
     1441 attttaataa tcatttactt acttttcatg attacacact ttttatgatt accctgtatc
     1501 ttggtgatag aaaaagtgag acttacgctt tgcaatggaa atatattgat tttgaaaaac
     1561 aaacggtccg tcttaaacat actttagata aatatcaaag aaaaaagttt acaaaaggtc
     1621 gaaaagatac cgtcattcaa gtaccagaag ttgtaatgac attgctaagt gaatggaaat
     1681 cggtacaagc tgatcaattg ttgaaattaa aaattaagca aacgttagat cagtatctct
     1741 ttacttatac taaaccatct ggagaagtaa attgtcctgt tcatgccgat tacctaaact
     1801 atcgaatcaa ttcaattaaa agaaggcatc ctgacttagt tcatttatct ccacacaaac
     1861 tccgtcatac ttatgcaact atagctagac aaggtggagc agatatgaat caaatttcaa
     1921 atgctttaac acactcagat atttctacaa caaagatata tgtgaacaca ccagatatag
     1981 ttgataaggc tgtttttgaa gcttttcaga gaggattgaa taagtgtgat tgatatgtgt
     2041 tcttaaatgt gttcttaaaa acaaaagaag cactttccaa ataattgaaa agtgccagaa
     2101 acgttgatat ataaaggata ttaacgtttt gagaattgtg aagctttacg tgctttctta
     2161 agacctggtt ttttacgttc gaccatacgt gcgtcacgag taagtaagcc agcacgttta
     2221 agagctaaac ggaagtcagg atcaacttct agcaatgcac gagcgatacc atgacggata
     2281 gctcctgatt gtccagcgta tcctccgccg tttacgttaa caaatacgtc atatgcgcct
     2341 tttgtttcag taacagcgaa aggttgattg attacttcac gcaagtcagc atgtggaatg
     2401 tattcttcaa catctttttt atttacagta attttgccag ttcctggtac tagacgtacg
     2461 cgagcaactg cgtttttacg acggcctgtg ccgatatatt gaacttgtgc caatgaaatt
     2521 ccctcctatt agattaagtt tgtgatgtct aaaacttctg gttgttgtgc tgcatgtgga
     2581 tgttctgctc caccgtatac gttcaatttt gtaaattgtt tgcggcctaa agtgttttta
     2641 ggaagcatac cttttacaga agtttcgatc aaacgacgag aatttttagc acgcaattcg
     2701 ccagcagtga ttgatttcaa tcctcctggg tagttgctgt gacggtaata aattttgtca
     2761 gtcgcttttt tacctgttaa tttcactttg tctgcattga tgacgatgac gaaatcgcca
     2821 gtatccaaat gaggtgtgaa agttggttta tttttaccac gtagtacaga tgcaacaact
     2881 gttgaaagac gtcccaatgg aacatcagtt gcgtctacta cataccattt acgttctact
     2941 tcgcctggtt tggccatata tgttgtacgc acgttatttt cctccaattt aagttctgat
     3001 gtttgacata cacttcgagt ttccggggct ctttgtgggg caaacaatac catttaacat
     3061 attacccgct aatctagtca atgtcaacgt ttttctgctt gattgaaaaa tttatatgaa
     3121 actttacttc ttttctttct tttatataaa agggtcgttt cctgtcaaaa tgaaaaacaa
     3181 aaagaggctg tgacaaaagt cttgtcacaa ccccttattt tgaataaacg gtgttctcct
     3241 gccaactcct cggtcacaag tcacaatatc cataaaacat aagaagctgt tttttaaaat
     3301 tgtttcttgt gactcggagc tgaacggcag gcttacaacc tcttggtaaa cggtgttcaa
     3361 tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgacgtt
     3421 tgtttctcta atactcaggt caaaacgctg atttcacaac ctctttttcg gtcaagttca
     3481 acaacagttt ttacgcgtcg atctctggcg ctttgcccaa atgagcttgg atcatccaga
     3541 tacgtttttc tgtggctgtt ttgaattcga taaagatatc ttgtgttgga tcgtcgcctt
     3601 cttctccaga aacttcgatt cctttttggt aaagatctgc tagataacga tagcctgcaa
     3661 caagtgtttc caaacgttct ggaatagtac gatcccagcg gccaatttca tcagggattt
     3721 ttgtgttatc tgcaaattca cgcaatgtag aataaggtga accgtctaat gtgatcaagc
     3781 gttcggatac ttcatctaac tgagcgttca aatcatccat gaactcatcc atcattgggt
     3841 gcaatgtaag gaaacctggt cctctcatgt accaatgtgt ttggtgaaca acaacagaca
     3901 tttggcttag atctgcaact aattgattta atacttcttt ggtttgttgg aatttcatct
     3961 ttcttgcctc ctaacatttt cttacactta aaataatagt agactttcaa tgaaaagtac
     4021 aacaaaatca tttacctgag aacgattttc agttcgcttt tttatccgta aagccaatca
     4081 tttttcagaa tcagctaatt cttctcgcag ttgagcaaat tgctgataag tctgtgctaa
     4141 atgcttttgt gtcgaaacaa ataatggata gattttctga tattttttat gatcagtttc
     4201 ttgtactcca acttgctctt tcaattctat caattcctgt gcttcactga tactttggat
     4261 ctttccgatg cttctccagc ctaaaatcac agctcctaat gcggatgctt ctgtgacagt
     4321 agggaaaaca agcgggtaac ccagtacatc agccatcatc tgacgaaaaa tcggagattg
     4381 agcaaacccg cctgtagcac ggatctctgt gagctgattt tcctggcaaa ctccttttaa
     4441 aatccgataa aggttgagac agatcccttc tagaatcgcc ttcatcattt ctttttctgt
     4501 atgttgccgc tgtaaaccga taaaactgcc aaacgcatct gcgtcccaaa gcggtgctcg
     4561 ctcaccaagt aaatagggat gaaaaatcag accattggca cccgctggca caaaagcgat
     4621 agccgaaagc agtgcatcat atggattttc tcctttttct ttagcagatt gaacgacttc
     4681 ctgtaaaaat gtatgccctg cccaatcaaa gatgccagca ccattgctag tcgctcctcc
     4741 cactacccaa tgtttctgat ccagaacata acaaaacgtc tccatttctg gatggaactg
     4801 aggctgagta gtaataaaac gaagtgctcc gctcgttcct acggtcaaag cggcgatcgt
     4861 atcaccaaaa gcacctaacc ctagattaga aagcggcccg tctgcccctc ccaaaacaaa
     4921 tgatatatca tttggcaaac ctagttctgc tgctgttttt gatggtagac ctgttacttg
     4981 ataggtggaa ggaaagactt gcggcaattg ctctattcga atgttcaagt agtcaagcac
     5041 ttcttctgcc cattggaatt catggatatc aaaatatcca gttcctgaag ctgttccgta
     5101 gtcgctgatc agttcgccaa acaatctata aaagatatag tctttgatac cgacaaaata
     5161 agcagttttt ttgaataatt caggttggct gttttgcagc cacttgattt tgttcaatgg
     5221 tgtcattgga tgaatcggcg tcccagtctg ccgataaaat tctttgccaa gcacggtata
     5281 tttcagtttt tctgcttcgt ccgctgctcg gttatctgcc caagtgatca ttcgagataa
     5341 cggtgttttc tcttcatcca acaatatcag gccatgcatc gcagaggaga aggagacaaa
     5401 ttgaatgatt gtgtctgaag gcaactgttt aattacttct tttataacgg tgcagacacc
     5461 ttgaaagatc aattcctgat cttgttctgc catcccttgt gtttcttgga tcaaagggta
     5521 gcgaaaataa gcggatgctg cttcttgggc ttcatctgtg aaagcaactg ctttggtttg
     5581 tgtggttccg atatctaatc cgattgaaac gttcattttt tcacctcaaa ttttatgtat
     5641 gtacaaaaat ttagtttaag cttgcgtatc ttggttttga aaagggggag atggcgtgtt
     5701 actttatttt atcattggtt gctgtatcgg atcattttta tgtttaaccg cccagcggat
     5761 tccgttaggt cactctatta tttatcctag atctcattgt gtcaaatgct gtcgttcgct
     5821 ttcttggtat gaattgattc cgattctctc gatcatcgtc caacgatttc gctgccgcta
     5881 ttgcaggtgt cggttgccct tctactattt gttggcagaa gccttatgtg gtggtctttt
     5941 tgcctggttc tttacttttt cttccgcaag aaatttttcg acgttcatat ggatgctttc
     6001 ggctttgcta ttttcgttga tggatttatt ttacttcatg gtccattctc acacgctatt
     6061 ctgttcttgg gcaattttat ggattttttg gttgcaggcc ggcgtcttcc aatggcaaag
     6121 tgtgctgtta acgtttatcg taggcggtgt gtgccttcgc tatggtcaat cctatctcgg
     6181 agctggcgat actctgttga tgctttcttg gagtggtggc ttgtctctag aagagttgct
     6241 tcagatcttg tttcttgcta gtctgtttgg aatatccgtt ttttctgttt actttatctg
     6301 tcataaaaaa aagttggaaa agctgccttt cattcctttt ttgagcagtg cgttgctgat
     6361 tgtccttcat cttaggtatt gaaaaaaggt tgtgacaaac gacttgtcac aacctctgat
     6421 atatagtgtt ctcttaccaa atccttgtga cttggagcta ggcgacaggt tcacaacctc
     6481 tccactaaat ggtgttcaat cagctgaccc tcagtattag ctcaaaatgc tggttttaca
     6541 acctctgata tacggtgttc cataagtagc tttttcggct tcttatttct tgacgcccca
     6601 atgtcgacga aggaaacctg cacgtccaga accttctcga taaagattgt aatggagctt
     6661 gtttttcttg taataatcct gatggtaatc ttctgcagga taaaattcgc ttcttggttc
     6721 gatttttgtc acgattggct tatcgaatcg cccgctgtct tgcaatgctt gttttgaacg
     6781 ctctgcaata tttcgttctt cttcatcatt atagtagatc actggacgat aagaatcacc
     6841 gcgatcagcg aactgtccta atgcgtcagt cggatcggtt tgctgccaat agatttccac
     6901 caactgatca taagaaataa cttctggatc atattcgatc tctactgctt ctgtatgccc
     6961 tgttgtgcct gttgttactt gttcataagt tggatttggt acatgcccgc cagtataacc
     7021 ggatgtcaca gaaatgatcc ctggctgctc ttcaaatggt ttgaccatac accaaaagca
     7081 tccgcctgcg aaaactgctt tttttgtcat ataaaatccc ccttttttct catcatacct
     7141 tgatttccaa aaggaggata acgttttgct tgtgacagaa aaaacggaaa ctattatata
     7201 cgtgatacgg cttctgataa atgccaaaac gaacgtactt gatcaaaaac ggttaaatca
     7261 ggaatcacat attcagcaga aggaagattt atgttttcac ctattcccaa tacatgcatg
     7321 ttaccagcca aagctgcttg acaaccagct tctgaatctt caatgaccaa gcatgcattt
     7381 ggcggtacat tcaattgctg tgctcctttg agaaaaactt ctggatcagg ttttgctttt
     7441 gatacttgcg tgccgtcaat aaggacatca aaataacttg tcaaattcgt tttttctaaa
     7501 atgaggcgtg catttttgct tgctgatccc aatccgatct tgatgtttgt ttttttcaag
     7561 tactcaagaa cagctattgc accaggcaaa acggatgttt catccatttt ttgaatcgct
     7621 tgaagatata gattgttttt ttgctcagca aaggctgatt tctctttttc tgagaatttc
     7681 tgttccattt ttccaaatga cagcaatcgc tctagtgact cggttctgga tattcctttc
     7741 agttgctcgt tttctttctc agtgaatgga atggacagct ggttagctaa aaccagccac
     7801 gcttcgtaat gataacgagc tgtgtctaca agaacgccat ctaaatcgaa tagtgctgct
     7861 ttgattttca tcgattacct ccttgaaaat gaacgacttg aagtaaagat gcgatgtttt
     7921 tagctgtgcg atgaagatta tcagtggctt gctgtaaagc ttcttgtaaa ggcacagctg
     7981 caggaaggat cggaaaaacg gccgaaatgc caacatctgc tgctttttcg cttcctttac
     8041 caacacttcc gcaaatagct atcacaggaa cgccctctgg tactcttcta gcaatcccaa
     8101 ctggcgcttt tccttttaga ctttgtccat ctagacgtcc ttcgcctacg attacgaggt
     8161 ctgcttgctg tacccgttta tcgaactcca gtaaatccaa tacgtagtcg atgccagaga
     8221 ctattttccc tgaagcaaat gctaacagtc ctgcacccat tccaccacca gcactactgc
     8281 ccgatgcctc gattacttga ggggcaaatc tcatataaaa atcatgcatg gcttggtcag
     8341 ccactgctaa ttcattttct ttcaagcctt tttgtggtcc gaaaacaaaa gaagcgcctt
     8401 cttttccgca taaaagatta gtaacatcag taatgacagt gattttggct ttaatcagct
     8461 caataggcac gttcgtttta tcaactgtag caattttacc tagattttct cccagtgctt
     8521 ctactttttg attatcttga tcgtaaaaag cataacctag accttctgcc atgccgatgc
     8581 cgccatcgtt ggtactactt ccgccaacgc cgatgatgat gtgtcgaaca ccttgttgca
     8641 acaattgtcg tatcagttct cctactcctc gcgtggataa cctaagtggc tgttgttggt
     8701 ttagtgggac atctttcaat ccgacgattt ctgccatttc aaaaatcgca gtttgaccgt
     8761 ctgaagcgta ctggatttct ttgtttttgc caaatgcatt agtaacgact gctgtttgcc
     8821 gcttcaacct taaactgtct gctaatgatg aaaccgttcc ttctcctcca tcgcctatag
     8881 gtagcagatc atattcagcc tgaacaaaaa cttcttgaaa acctgattga atggcttgtg
     8941 ctgcctctaa ggctgtcaga ctttctttga aagaatctgg cgcaatcaga atacgcatta
     9001 ttttcccctc cgttccaaga tcagttcttt ttccagctgg tattggtctc cattgacaaa
     9061 aataggtaat gtttctttcg tctctttttt tattactatt tgtttttttg tcaaaacaaa
     9121 tagcaacctg tgatttttcc aaataaaagg aaaagaaatt ttttcccatg ctgaaggaag
     9181 tcttggagaa atagaaagag ttccattcgt tactgtaata cctgaaaaac caaatataac
     9241 tgctagccat agtgctccta atgaagcagc atgaattccg tcatctgatg aatgcggatc
     9301 tggtcctaaa tcaatcaggc acgcttcttg gaataaatga tacgcccatt ccatttcttg
     9361 gatacggcaa gcctctatgg cgtgaatcgc tttactcaac gatgagtcgt ggattgtttt
     9421 gttttcatag taatgcaaat tcttagaaac gattggttga gaaaaatact caggtaataa
     9481 gtataacagc atgacaagat ctgcttgctt gctgacttgg agttcattta cttcgctacg
     9541 ggcataatca agtaaaatag actggctgcc aggacgttct ttgtaagtgg tcaaatcaat
     9601 aactggctta ctgagatacg tgtcatcttg cggaatcagc tgttctttgt taggcactgg
     9661 caaatataac ttatctaaaa aattttgaca ttctttcaaa aaaagcggat cgccagcttg
     9721 atatttaaca gctaaggcca cattataatg cgccagatag ttcgtatatg cattattgtc
     9781 gatatattct gtgtattcat ctggaccaat gactctgtgt atctctaaat gatcatcgac
     9841 ttttacagct ctgctcagcc aaaaagctgc tgtttcatga atgagcgttt ttccctttgt
     9901 ttctaaaaac gattgatcct ctgttgcttg gacatactct tgaacggcat aagcaaggtc
     9961 tgcactgata tgatgttctg cggcagctga agcaacttct tgtcttgttc cggttttgat
    10021 attcaatgca gcaaatttgg gtgtctcttc ctgaccagtg tgtgcgcttt cccatgggaa
    10081 aagtgcacct tgatagtgat aatctcttgc tttttgccgt gcgccttcta atcgatcata
    10141 acggtacagc agcaaattct ttgctacttc aggaaactga tataaaaaga aaggcaggat
    10201 aaaaatttcc gtatcccaga aaacatgccc tttataacct tcgccagtta gtcccttcgc
    10261 ccctacacta acacttggat cattgcgggg agtcataatc tgtaaatggt aagcagcgaa
    10321 gtccatcgct aactgatcga aagaagaatc gccttggatc gttatccgat gctgatgcca
    10381 aaaatctgtc caataagaca tcgactgctc taaaatttct gtataactaa cgcttttctg
    10441 ggcttgggca atacttttct taaacgggtc tgtttgctga agagaggtag agagcacgat
    10501 gatcttctcc agcgtaaagg acgtattttt tctgatcttt tctgaaaagg aagcggtgat
    10561 ccggcgattt tttgcttgga atacacctgc tttattgaat ttagctgcaa tcccaatatc
    10621 aattttactt tctgttgtct ggtaggatgc actgatcaaa tcgtcaccaa atactcgcag
    10681 ttctttttca accaactgct gtgtaccaaa attggtttgc tgtccatcga tacccgtttt
    10741 cacatctact tggcagtctt cggtcaacgg cttgatgacc actttcaggg ctgcctgatg
    10801 gagattattt tgatcagcaa cacgacaagt aatgacttcc actttttttc cttgagcagt
    10861 ttcaatgagc gcatagcgtt taagctctcc catttctaaa tctagtgccc gctcgtatgc
    10921 aagaactttc atcgtctcaa gagaaaaatg ttcaccgtcg atttttattt cccatctgat
    10981 gacatctggg agattcacta actcggctga ttctgctcct aaaggtcggt tatatacacc
    11041 agctgcaaac attcccctta cttgattccg atagtcttcc tcctggcttc ctctgatacc
    11101 aaaaaatcca tttccaatgg tcatctgact cgcatactta ttgatatact ctaaatcaaa
    11161 ctgtttttct tctactttca taaggtaata acttctcctg tttctgccga acgatataaa
    11221 ttttccacca gattctggat gacatatcct tgccagccat ctgcaatcat ctgagtctgg
    11281 tcaccgagcg cccgattcac aaaatgagcc atacttagtt catgtgggtc attaactggc
    11341 gtggtctttt gtaaggaaac cagctttcca tctacatctt cgaatagttc gcctggatac
    11401 agattagcac ctgcctgatc gccacaaaaa gccacattca gtttgtcttc tctgatattc
    11461 aaagcaaacg aagtattcag ttggatgatc ttgccgtttt ctaattccag tgtgccaaaa
    11521 attgcatctt ctactgtgaa tttgcttgga tcccatttcc cgaatttccc tgaatttttt
    11581 ttcgtaccaa ttttttggaa gctgtacgcc cgaacgcttt ttactttagg aaatccagta
    11641 atatacatcg ctgtatcaag catgtgaatg ccataatcga tcaaaggccc gcctccttga
    11701 agttctttgt cgataaacgt tccccagcct ggaacaccag accgacgaag cgcattcgct
    11761 tctacgtaat aaatatcccc gagaacatca agatgattct tgatcattgt tgcttcgtca
    11821 gaaaaacgat gctggaatcc ataagccaaa atctgcccgt ttttatcggc tgtctctgcc
    11881 atctttcttg cttgatcagc tgtcatagct ggtggtttct cgcacatcac tgaagcatta
    11941 gcttcgaggg cagccaatac atgttcgaag tgaaagcgat tgggtgtaca gacactcacg
    12001 atatcgggag aaatctcatt cagcatttgt gtaactgatg tatacgcatg agaaaatcct
    12061 gtacgtcggc aaaagtcttc tgctcgctgg ctgactggat caaccacagc ggccaattct
    12121 aagacatctg atcttttttg ataataagca gcatgtactt tttctgctac ttgtccagcg
    12181 cctatgattg ctactttctt catttatttc tctctccttt gcttatttac ttattgttat
    12241 tctttctcta ttctttctct aaatcattta aatgacgcgc tgtttccaga cagtttttca
    12301 agtattgcaa tgattcctga taaattttca cttcattttc tcctcgcaaa cgacattcat
    12361 ataccatata accttcatac tcattttgaa gtagcgtttg aaaatggcga gtaaaatcaa
    12421 ttgcgccaga tcctggttgg tagcgatgat tatctgctaa atggatatga cccacatact
    12481 ctttttgcgt agtcaatgct tttgaaatat catcttcttc aatattcata tgataaaaat
    12541 cagcgatgat ctgcacatgt ttcaattgat tagagtcaat gtaatgacgc gcatccgcta
    12601 acaaattgat catatggtct tggtatcggt tcaaaggctc caaataaata accgtacctg
    12661 tttcttcggc tactttatct aaataacgaa gtgattcgct gactgcttta tagtctcctt
    12721 ctttgcttcg gggtgaaacc atcggcggta atcgataagt gaacattccc caagctgctg
    12781 ggataacgat tcctttgcct ccaacctcgc ttagtgcacg caaaatctca gcaatttgac
    12841 gtacaccatt aagacgccgt tcttcgataa agtcaccgat ccacccatcg tagccgttac
    12901 aagctgtcac tactgggatc cctgtttctt tgattgcctg tttgactgtg tcgatatttt
    12961 ctattaacaa cttaccgtcg atttcgtatt cttcaaatcc acagtctttg ataaatctaa
    13021 atttttctaa aatgtctctt gggaaaaatg cttgatcttg cgtaccgaat ttcattgttc
    13081 tcctcctcgg acttgaacgc ccattttgat actttcttct gggtgctggt ctacataacg
    13141 catgtaagct tctgcgcttt ctgcaaatgg cacaactgga tcaatgattt cttcacaatc
    13201 caagtaaccg ttcatcaata attcccaaca tgtgtcctcg attcgtttac ggttccatct
    13261 tgggtaatct gggtttggtt cactagatgc tcgagcaaag acgatttttg cattattgaa
    13321 atgtgcttcg cgaccaagat taaacccagc tggaaaaggt ttagcaaagg caacataaga
    13381 aatgatcccg ccatagccga tccctattaa agaagcttgt aatgcggcag cactgccact
    13441 cgtttcaata actgaatcta ctccacgttt atctgtcaat tcctttagac gaaaaccgac
    13501 atctgctttc ataggatcta gcgtgtaatc ggcaccgtgt ttttctgcta attctctacg
    13561 ctgcgctatg ggatcgatcc caacgatggt agtagcacct gcttttttag ctaattgaat
    13621 cgcaatttgc ccgatagcgc ctaaaccgaa gattgctaca gcatcacctg ctcgaacatg
    13681 tgcttcccgg ataccaccta aagcgaattg agcaggatcg taacatactg catttttcca
    13741 ttgatccaac cgatcaagtt tacgtagttt ataattatca acaccgtttg taatcagcgt
    13801 ttcacgaatg ggtccataag tgcagactaa ctctcctttt tgatactctg tcaccaaact
    13861 gcccgtttct tcgatcgtac cgacaatcat atttcctaat tgaaattcgc caaataccac
    13921 accgcgttgg cttccttctt ctcttggcaa gaataggcgc cattcttctg aaaattcttc
    13981 atcgataaat ggacttaatc ctctaaagtc gacaatttct gttcctggtt ttggagtagc
    14041 gaaaattact cggatacgta cttcgttctt ttcgatctca cggtcttcgt aatctttgaa
    14101 tccggctatt cttggttcta atgcaactaa ttttttcatc ttttctttcc tctttctttt
    14161 ctttttatcc ttttacgccg ccttcgacta aatcaccttt aataaatcgt tcagataacg
    14221 catacataat gatgactggt aaagcggtca ccaatgaagc ggccatcatt cgtccccaaa
    14281 tgtagtctgg tgtactgaat aatgtgttca aaccgattgg taaagtaaat ttttcggagg
    14341 tcgacaggaa aatcgaagca aataaataat cattccacga aaccataaag caataaacga
    14401 agacagaaat gattccagaa atagccaatg gtataatgat ccgaataatg atctggatcc
    14461 ggctaagacc atcgatcatt gctgcttctt caatatcagt cggaatcgtg tcaaaataac
    14521 tttttagcat gaatatcgca gtaggaagtg tttgaacgac catcgtgata atcagtgctg
    14581 tattggtatc atacaaacct agaccggaaa tgattttaaa taatggaaca acgatcaaaa
    14641 taccggaaaa catataaacg gtgtaaaagc tagcattgat tttcgagcga gctttgaagc
    14701 gtagttttga caaagcgtat gccccgctga tcccaatcac tactgcagtt acggaggcta
    14761 tcaaggagat cactaaacta tttttgaaat acatgatgaa tggaaaaatc tcagcattga
    14821 aaatatcctg ataatgttca aaagtccatt ctttcggaaa aatagtttgt gtcgttgcaa
    14881 ttgcttcttg tgaatccttg aatgatgtca tcagcatgat caaaaatgga aacaaattga
    14941 cagctaatag caacaaaatc gtcgcgtaaa ataagatttt cctgctcttt ttatttttca
    15001 tttcaagtcc accttctttc gtgagagcaa gacgaccgct gcaataatga tgaacaatag
    15061 aatcgatata gctgctgctt ttccaagatc attgaacgca aaggctgtct gatacaaata
    15121 aacgcctaag atattgactt tattcgtcag tagatagaca tctgtaaaca tatagaacat
    15181 ccagatagat cgtaaaacaa tcacagttgc tagtactggt ttgattgccg gcagtgtcac
    15241 gatcttgaac ttctgccaag catttgcacc gtccatttca gctgcttcat aaagcgtttt
    15301 gtcgactgtt tgcaaaatag ccaaaaatga aataaacgca tacggatagt atctccagat
    15361 agcaaacaaa acgaccataa agaagctagc aactggctgg tcgaaccaca gcggtgcttc
    15421 gttgaaaagc tgcaacctat ctactaacag atcattgacg attccataac tattattgaa
    15481 catatatttc caagcaaaaa tcaaagaaat agatggcgta acataactta ataaaatcag
    15541 tgaacgcacc gttttcctaa atctgaactt gcggttaagc aaaatagcta cggctaatcc
    15601 taaagctgta ctgcctgcta caactaagaa tgtgtaagca actgttaaac ctaaagaacg
    15661 ataaaattca ctatcactta aaatttcaag ataattgctt aatccgataa aaacggcgtc
    15721 tgagttagga ttcagcggca tatcgaaaaa actgatctga atattggaaa gcattggaat
    15781 cagcactaaa acagctaata aaagcatgct tggtgctaaa agtcccatgg ctaattttaa
    15841 gtcagaatgt ttccttggac gattcattga aacctccccc tgataatctg ccaatagcta
    15901 tctacttttt tcgaacacca ttggcagaac ttcttgttat ttcgtatttt tttgtgcgtc
    15961 agctaaagct tgatcgacat cttcgtttcc gacagttact tggttcactg cttttccgat
    16021 cacacctgag cttgtgatat ctcccatttt cgtgaaattc ttttccccga ctagtccaaa
    16081 gacttgtact tcatcaaaag cactagcaat ctcagatggc agctcgccaa aagacttgat
    16141 gacttcattt tctttgtaac catccagttc gactacttgt ttattcactg gttgagcacc
    16201 accaggagac atcaagaccc acttttccat gtttttcggt tgagataaga aagctgtgaa
    16261 tgcttcagca gcctcttttt gtttctcgtc tagaccagaa gaaatcgtca atccagatac
    16321 tgtgccatag acagcttgat ctttttgtgt tgggatagca aaaccgatat tttctgagtt
    16381 ccccgcttca taaacagaag gcaaaatgta agttgaatag attgccattg gtgctgttcc
    16441 attcatgaaa gcatctttga tttctgtcac atcattagag cctggcatcg tgtattggga
    16501 taagttttga taaaacgaca atgcttctct catcttttcc gtatcgatcg ttacctcgcc
    16561 ttcatcatcc aaaacatttg ctccatttga caaagcaaat tgagagaacg cttgctcact
    16621 cattgttcct tcagcagtag gcatagcaat tccatattgt ttattctctt tatctgtgaa
    16681 atgttgagca atttttaaaa tatcatccca attttctggt tctgaaaaac cagcttctga
    16741 taatttttct ttgtcatacc aaataccttg aacccagccg ctaattggtg cagcaatata
    16801 gctttttcca tcttctgaac gaacaaggtt tttggctccg tcataataat tatcttctcc
    16861 agcttcctcg ataacgtttt ggactgccgt ctgatcaatc agttcatctt tatccatcac
    16921 tttagcaaaa tcctgactga cttccattac ttctggtaat ttaccggaac gagctaacgt
    16981 aacgattttt gtattgaacg ctgattcttc taccggcact tgcttgattt tgatgtctgg
    17041 attttctttt tcaaaatcag caaccagttc attgatcaca cttaatcgtt cctgttcgac
    17101 agaagagtgc atgaattgaa tcgttgtctg ttgtgattcg ttagagcttt gactttcacc
    17161 acaagcagta agaagaaatc cagtagctag cagacaggta gtaacaataa tttttttcat
    17221 ttttttccac ctcttatttt attattttca gccacatata ttcatacggc tggaatatca
    17281 actttcgttc ttgactgcaa tggataattt gtccagaaaa caggtctagg taatctcctt
    17341 ttggtaaaag ataacctgct gattgattgg aaaaattatg gataactaag actttctctt
    17401 ccttcgcttc gcgaataaaa ctgataaaag aatctgctga gtccagtaag taaatcaaac
    17461 tattcggatg gaaggctttt tctttttttc gaatagatag catctgggtc aattcaccat
    17521 atactgctgt tcttaatgga tctgtctgca actctttctc aatatctgct aacaaaaatt
    17581 tttgccgatt gatttttcga ttctctcctg ttttttctac gcctttgtaa tcatttcgac
    17641 ttcccagcac actttgaata taaattgccg gcacgcctgg taacgttacc aaaaatgaat
    17701 gagcagctaa aaagcgtttg cttctccatt ccgtcgtatc tgttggagaa cttaacgcat
    17761 ctagatacgt agcgtttatc tcatatggac tggtcgttcc atctgggttt ttcttgtagt
    17821 ttactcgggc accctctttc ttcagctgtt ccactaattc aaggatcgtt ttttccggca
    17881 gaatacctcg taccggattc aatccgatac cgtcatggga ggctaaaaaa ttaaaaaaag
    17941 ttgttttttt cgatgcgaaa tcgatggttt ttaaccattc tttcaactta cttgtattac
    18001 ccgaacgaat cgcttgaagg gtcaaaggtg gtagcgaaaa ctgatagacc atctgtgctt
    18061 cattctcgcc attcccaaaa taactgatat tatctttgtg tggcacattt gtctcagtga
    18121 tcaataccgt tcctggagct gcttcatcag cgattgcacg gaataattgg atgattttat
    18181 gcgtttctgg caggtgtata caagaagttc ctagttcttt ccataaaaat cccacagcgt
    18241 ccaagcgaat aaatgaagcg cctttttcta agtaaaacaa aagaatatcg atcattttca
    18301 ataaaacttc tggattttta aaattcaagt ctacttgatc ttcgctaaaa gtcgtccata
    18361 cgtgtttttc tttcccgttt ttgtcaatga acgttgtaag taacgggctg gtccttggtc
    18421 tagtcacgct gcttgtatct gtatccagtg gcaaatcaat aaaaaaatct ttgtacgtat
    18481 cattcaagtt taaatattcc tgaaaccaag tactttcggc agacatatga ttgcagacaa
    18541 agtcgaacat taaccgtgtt tcgcttctca tttcttctat atcttcccat gtgcccaaac
    18601 ggtcgttgat tgcctgataa tttacaacgg agaaaccatc atcagatgta tatgggaaaa
    18661 atggcaagaa atgtacaact ctaaatgtat ctgccaaata cttttgataa aatttcttaa
    18721 atgtacgcaa agttggttgc tgctcttcaa aaaattgatc cccataagta atcaagaaaa
    18781 tatcttcttc gtcccaatgc agtttgcctg cctggccttt cttctgctga taaacactga
    18841 ctttttgttc aatcatagag gcggttttct cagacaaatc tgagtcgcga taaatcgctt
    18901 gtaaaagttt tgtatacatc ggattcccct ttcttttttg ggagcgttcc caacgatttg
    18961 atcatattat ggaaaacgct tactgtcaat agcatttttt taaataattt tgatatacac
    19021 aaaaatacaa ataagaaagc ttatgtatca ataattaata gcttcttaaa aaaatatggt
    19081 ctctcatgat catgcaagct attcctaaat tttcaaaatc atagacaaaa aagaatgtct
    19141 attgtactat ggaattattc taaaacgctc ccaaaaagga ggtgcatata aaattgaaac
    19201 ctactattta tgacattgcg aaaaaagcta atgtctcaaa atcaactgtc tccagagtat
    19261 taaataacca gccaaatatc tctgataaag caaaagcaag tgtacttcaa gcaatcaaag
    19321 aattgaatta tgaaccgagc aaaattgcaa gaggactgtc aggaaatggt tttgacgcga
    19381 ttctagcatt atctcatcgt acttctcata cgacctatgg caatcctttt ttttcagatg
    19441 ttattcaatc catttctgcc gtttctgaac aaaataactt cgacttgatt ctacaaacgg
    19501 caaaaacacc agaagaagag ctagaaaaat gcgcgaaaaa gatccaagaa aaaatgatcc
    19561 gaggaattat tattttaagc tctacgatgg atgaaacact tctttttgaa ttggatcaat
    19621 ataatattcc tattgtggta atcggaaaag tcggaaacga ctacacgaac atctactcag
    19681 tggacacgga taatttccag gatagttacg atttggtgga aacactgatc aagaatggac
    19741 accgtcatat cggctgtctc catgctccga cagaagttta tgtatccaat gatcgtctca
    19801 atggttaccg tcaatgctta tttgatcacc acctagatat ccgtaatgaa tggattatag
    19861 acggcggctt tcgcttagaa gatagcatgg cagcaatgaa tgatttaatg aagctacgag
    19921 aaagaccaac tgcgattttt tctaccgatg cattaaaatc attgagtatt tacaaagtgg
    19981 cagaacaatc tggaaagcag atacctgatg atctttcgat cgcaggattc agtgatccct
    20041 tattgtcctc cctattttcg ccacagttga ctcggataga cattccgaca aatcagttag
    20101 gaatcaaagc cacagaactt ttattccaac taatccacca ccaataccct gaagaaaaaa
    20161 gaatcattct ttcaaccgag attactctca cagattcgat ccaacaacaa gaaatgtaga
    20221 acagaattat aaaagaacag aaacactgag gataagtcag ttactctgtt cttttttcaa
    20281 tcactcatcc ctcgttaccc cctcccggta ttttagcgaa tttttcttat tttccatctg
    20341 ttttagatag ctgtggataa ctataatgtt tgtgttaaac tattttttcc acagggaaga
    20401 aaaaactatt tttaaccacg ttgtgtataa gtttttatag ttcgtttttt taaacacatt
    20461 tacccttatt tcaatccatg ttatttgggg atacttatct ctaaaacata aatagaataa
    20521 cagacatgtc tactggtttt gttttattcg aggaattatt caataatagc tagcaaaata
    20581 ttaaaaataa ccgctctatc ttcccatcaa tttattttgc attcaaaaaa tgatctatct
    20641 ttacttttgt tgattgactt ctcttggaag atgatcgttc cgatacctta attggatacg
    20701 aaccgatgtt gttaagatcc tttcccactt ttcttctagt tagacaaatt attttagaca
    20761 aaccaaaaaa gaggttgtga caataatttg tcacaacctc ttttttaaaa tatctatttt
    20821 gtttgattgt tcattgcgtc ttctgcagcc atttgcgctt caatatctga aatactgtag
    20881 acattttcgc ttgctacacc gacttctttt ggttccatgt cacgataacg ttgcatacca
    20941 gtaccagctg ggatgatctt acctataata acattttctt tcaaaccaag aagaggatct
    21001 ttcttaccgc ggatcgcagc atccgtcaag acacgagttg tttcttggaa tgatgcagca
    21061 gataagaaac tatttgtttc taatgatgct tttgtgatac ctagtaagac aggtcgagaa
    21121 gttgcaggaa cgcctcctga gactagcgta ttgtagtttt gatctttaaa gtctgcgatg
    21181 tctaataatg tacctggtaa gatatctgta tcacctggat ccattacacg aactttacgc
    21241 agcatttgac gaaccattac ttcgatatgc ttgtcgccga tttctacccc ttgcatacgg
    21301 taaacacgtt gtacttcacg aagcaagtag ttttcaacag ataatacatc gcgaacttgc
    21361 aacaattgtt ttggatcgat tgatccttct gtcaatggtg caccacggtg gatcatatcg
    21421 ccttctgcta ctttcatacg tgctgtataa ggaacagtat acgtacgtgt atctgttttt
    21481 cctttgattg ttacttcttt ggtacgtgtt gcaggatctt ctgagatatc gatgacatcc
    21541 cctgttactt ctgtgatcac tgcttgccct ttcggattac gtgcttcaaa gatttcttgg
    21601 acacgaggaa gaccttgagt gatatcgtct ccggcaaccc cacctgtatg gaatgtacgc
    21661 atcgtcaact gagtaccagg ttcaccgatt gattgagcgg cgatcgtacc aactgcttct
    21721 ccgacttcaa cgtcagagcc agttgcaagg ttgcgtccgt aacaatgttt gcagacacca
    21781 tgttttgtat tacatgtgaa gacagaacgg attgtcactg tttcgacacc tgcatcaacg
    21841 atttgttttg cgatgtcttc ggaaatcaat tggtttcctg caatgatcaa ttcatttgtt
    21901 tcaggatgac gtacttcttt acgggtgtaa cgtcccacta aacgatcttc taatgattcg
    21961 atgatttcat ttccctcgcg gatggcttgg atttcaagac ctcggtcagt accgcaatct
    22021 tcttcacgga tgattacatc ttgggccacg tcaaccagac gacgagtcaa gtaacctgaa
    22081 tcggcagtct tcaaggctgt atcggtcatc cctttacgag ccccgtgagt tgagataaac
    22141 atttccaaga cggatagtcc ttcacggaag ttcgaaatga ttggcaattc catgattcga
    22201 ccattaggag cggccatcaa accacgcatt ccggcaagct gtgtaaagtt ggaaatgtta
    22261 ccacgagctc cagaatcaga catcatgaag attggatttt tcgcttccaa accttccatc
    22321 agtttttgtt ggatgctgtc ttttgcagca ttccaaacag caatgacacg ttcataacgt
    22381 tcgtcatctg tgatcagacc acgacggaat tgttttgtaa tagtttcaac ttgtttatgg
    22441 gcattttcaa tgatttcttg tttttcattc agtacactga tgtcagcgat ccctactgtg
    22501 ataccggcat gtgtcgaatg tttgtaccct aagtctttca tacggtcaag catcttagaa
    22561 gtttctgtta ctttgaatcg tttgaagact tctgcgatga tgtttcctaa gtttttcttc
    22621 ttgaacggta atactaattc ttgctctttg atatgtgcag gaatatcagt accagcttca
    22681 acaaagtatt tgtccggtgt ttggacggtc aagttgaagt ctgttggttc attcagataa
    22741 gggaattcag gaggcatgat ctcgttgaag atgatcttac caactgttgt gatcatcgta
    22801 cgttcttttt gccattcagt aaatggtttt tcacctaatg agttagggtt cacaccgata
    22861 cgtgaatgta aatgaacata tccgttgcgc catgcgataa cggcttcgtt ttgatctctg
    22921 aagaccattc cttcgccttc gcgtccttct tcttccattg tcaagtagta gttacctaga
    22981 accatatctt gagaaggagt aacaactggt ttaccatctt tagggttcaa gatgttttga
    23041 gcagctaaca tcaacatacg tgcttctgct tgtgcttctt cattcaatgg aacgtggaca
    23101 gccatttggt ctccatcgaa atcggcatta taagcttcac atacaagtgg atgcaaacgg
    23161 attgcgcgtc cttgtactaa aactggttcg aatgcttgga taccaagtcg gtgaagcgta
    23221 ggtgcccggt tcagcaatac tgggtgttcc ttgattacgt cttctaaaac atcccatact
    23281 tcgtcttctt gacgttcgat tttacgtttc gcatttttga tgtttgaagc tagttcgcgt
    23341 tgaacaagct cacgcatcac gaatggtttg aataattcaa tggccatttc ttttggaagt
    23401 ccacattggt acatcttcaa gaaaggtcca acaacgataa cggaacgacc tgagtagtcg
    23461 acacgtttac ctagcaagtt ttgacggaaa cgaccttgtt tccctttcaa catatgagaa
    23521 agagatttca atgggcggtt acctggtcca gtaactggac gtccgcgacg accgttatcg
    23581 atcaatgcat ctactgcttc ttgcagcata cgtttttcat tttgaacgat gattccaggt
    23641 gcattcaagt ctaacaaacg tttcaaacgg ttattacggt tgattacacg acggtaaaga
    23701 tcattcaagt cacttgttgc aaaacgtcca ccttctaatt gaaccattgg gcgtaaatct
    23761 ggtggaataa cagggataac gtccataacc atccagcttg gttgatttcc tgaagcgcgg
    23821 aatgcttcta aaatatccaa acggcggatt gcacgtgtac gtttttgtcc ttgtgcagat
    23881 ttcaattctt ctttcaattc ggctgcttct gcatctagat caacattatc aagtaattgt
    23941 ttaatcgctt ctgcacccat agcagcttgg aattcttgtc catattgttc acgtttttcg
    24001 cgatattcgc gttctgtcaa caattgtttt ttctctaaat tcgtatttcc tggttcgatg
    24061 actacatatg aagcaaaata aatgatttct tccaaagcgc gtgggctcat gtctaataca
    24121 agacccatac gtgaaggaat ccctttaaag taccagatat gtgaaacagg agctgctaat
    24181 tcaatatgtc ccatgcgttc acggcgaact ttcgaacgag taacttccac tccgcatcga
    24241 tcacaaacga ttcctttata acggatacgt ttgtattttc cgcaggcaca ttcccagtct
    24301 tttgtaggac caaaaatccg ctcacagaac aacccttcac gttcaggttt taaggtacga
    24361 tagttaatcg tctcaggttt ttttacttca ccgtaagacc agcttctgat tttttcggga
    24421 gaagccaaac ctatttgcat actttcgaat ttatttacat cgatcaaaag gggttccctc
    24481 catttcattt aatggcaaac cgcaagacgg ctgatcttcg accgccttgc agctcataga
    24541 ccggattagt ctaaattatc ttctgcggtt tcaaagtttt ggatgacatc gtctttttca
    24601 tcttccactt gttcagcagc tttcttttct aattctttgg cagtttgctg ttcggcaaat
    24661 tttgtcaaag catcaacagt gatcaaatcg tcgtcttcat cgtccatgtc gcgaagttcg
    24721 atctctgttt cttcgatatc caagacacgc atgtccagac ctagtgattg caattctttc
    24781 accaatacgc ggaatgattc cggtacacct ggttttggaa ttggttcacc cttgacgatt
    24841 gcttcgtatg ttttcacacg tccgactacg tcatctgatt tgtatgtcaa gatttcttgt
    24901 aaagtataag ccgcaccgta agcttccagt gcccatactt ccatttcacc aaaacgttgt
    24961 ccaccaaatt gagctttacc tcccaatggt tgttgtgtaa ccaatgagta aggtccgatt
    25021 gaacgtgcat gcaatttgtc atcgaccatg tgagcaagtt tgatcatgta catcacaccg
    25081 acagacacac gtccatcaaa tggttcacct gtacgtccgt cgtacagaac agttttcgca
    25141 tcgctggcca taccggcttc acgaactgtt tcccatacgt cttcatcact tgcaccatca
    25201 aatactggcg ttgctacgtg gatacctaac tgacgtgcag ccatacccaa gtgtaattcc
    25261 aatacttgcc cgatgttcat acgtgaaggc acccctaaag gattcagcat gatatcgatt
    25321 ggtgttccat caggtaggaa tggcatatct tcttccggca taatacggga tacaacccct
    25381 ttatttccgt gacgaccggc cattttatcc ccttcatgga ttttacgttt ttgaacgata
    25441 taaacacgaa ctaacatgtt tacgcctggt gacaattcat ctccagcttc acgagtaaag
    25501 attttcacat cgtgaacgat accgccgccg ccatgtggta cacgtaatga tgtatcgcgg
    25561 acttcacgag ctttttcgcc aaagatcgca tgcaatagac gttcttctgc agataattct
    25621 gttacccctt ttggcgtgac tttccctaca agcaaatcgc catcttggac ttctgcacca
    25681 atacggatga tacccatttc gtctaaatct tttaatgcgt cttccccgac attagggatt
    25741 tcgcgtgtga tttcttcagg tcctaatttt gtatcgcggg cttctgattc atattcttca
    25801 atatggatag aagtgtatac gtcatctttt accaaacgac ggctcatgat gatcgcatct
    25861 tcatagttgt acccttccca agtcatgaaa ccaactaaaa cgttttggcc tagagccatt
    25921 tcgccttgtt ccatagatgg tccgtctgcc agagtatccc ctttttcgac tttctcaccc
    25981 aaatgaacga ttggacgttg gttgtagctt gttccggagt ttgaacgacg gaattttgtt
    26041 acatcgtatt tatctaatgc accgttgtcg cggcgtacgc ggatttcaga agcatcaacg
    26101 tattcaacga caccgtcatg tttacataac aaagcagctc ctgagtcatg cgctgatttg
    26161 tattccatac cagtaccaac ccatggtgct tgaggattga tcaacggcac tgcttgacgc
    26221 tgcatgttgg cacccatcaa ggcacggttg gagtcatcgt tttccaagaa aggaatacat
    26281 gctgtcgcaa cggcaactac ttgtttaggt gatacgtcca tgtagtctac tttatcaata
    26341 gatacttcta agttttcact ttgagcacga gccataacaa cgtcttgagc aaatgttcca
    26401 tcttcattca atgggctgtt tgcttgggct acgatgtaat ggtcttcgat atcagctgtc
    26461 aagtagtcga tttggtcagt aacacgacct gtttcacggt ctacacgacg ataaggtgtt
    26521 tcgataaatc cgaatttatt tactttcgca taactggaca aactattgat caacccgatg
    26581 tttgggcctt caggagtttc gatcggacac atacggccat agtgggagta gtgaacgtca
    26641 cgaacttcat aaccggcgcg gtcacgagtc aatccgccag gtcctaaggc tgaaagacga
    26701 cgtttgtgtg tcaattcgcc aagagggttt gtttgatcca tgaactgtga caactgagaa
    26761 gaaccaaaga attctttgat acttgccaca actggacgga tattgatcaa ttgttgaggt
    26821 gtcaatgttt cagtatcttg gatcgacatt ctttcacgaa ccacacgttc catacgtgct
    26881 agcccgatac ggaattggtt ttgtaaaagt tcaccgactg aacggatacg acgatttcct
    26941 aagtggtcga tatcatcgac attgccgata ccttccatca agttcaagaa atagctcatt
    27001 gaagcaatga tgtcggctgg acgaaccgtt tttacagctg cttctggata gccgtttccg
    27061 atcacgttta cttcacgttc agggtctctt ggtgaaaata ctttgatgac ttggacagtc
    27121 attggatctg tgaccacacc gtcttctgat ggatagtaag tcactgaatt taggccatta
    27181 tcaatgaaag gagctagtgt ttccatgact tggtgagtca gaactgttcc tttttcaacg
    27241 atgatctcgc ctgtttctgg atcaactaat gtttcagcta atgtcagatt caatagacgt
    27301 gttttcaaat ctaatttttt gtttactttg taacgaccaa cgtttgctaa gtcgtaacgt
    27361 tttggatcga agaaacgagc attcagcaag ttacgtgagc tgtctgctgt ttttggttcg
    27421 cctggacgca aacgttcgta aacgtctttc aaaccttctt ctgttcttga gtcgcttgcg
    27481 tttttatgca agtctttttc gactgtattg cgtaatgttt cgctgtcgcc gaagatttca
    27541 aagatcgtat catcggaacc aaatcctaac gcacgaacta atacggttaa aggaatctta
    27601 cgtgtacggt cgatacgtac ataagagatg tcttttgcat cagtttccat ttctagccaa
    27661 gctccacggt tagggataac agtagaaccg aatccttctt ttccgttttt gtcgactttt
    27721 ccatggaaat agacacctgg tgaacggact aactgagaaa cgattactcg ctctgccccg
    27781 ttgatgatga atgttccttg ttctgtcatt aatgggaaat caccgaagaa tacttcttgt
    27841 gctttgattt cgccagtctc gcggtttgtc aaacgcaatg ttacatgtaa tggtgcagag
    27901 tagtttgcat catgcgcacg agcttctgct actgtatact ttggttcttt caattcataa
    27961 tctacaaatt ccaaagatag attgccattg aagtcatcga ttggcaaaat gtcttcaaac
    28021 atttctctaa gtccctcatc taagaaccat tggtaagagt ctgtttgaat ttcgatcaaa
    28081 tttggtaatt ccaacacttc actgattctt gcgaaacttc tacgttcgcg atgttttccg
    28141 tattttacta cgtgtccagc caagctcttc acccctctaa aatgtcattc aaaaagacta
    28201 cccgatcaat tgttacaata atatttcaaa tattattttt aggtaacaac agacattttt
    28261 tgcggctttt aggcaaaaaa aaaccaaaaa tagaacattc tcttgaaacc ttacttcgtg
    28321 agcttctact tttgtttcct tatattaaag ccgaaacgga caatatttcg tttctgcctt
    28381 ttcttcatca gatagttttt gcaacttcat atactacact tattttctat aaaagtcaac
    28441 acttcctggc gtttcttctg ctttttcgca acattttctt aatgttttca attttatgtt
    28501 ttcaattatc tgacaattct taattgacag acctttttat ctcggttata ctttagaaaa
    28561 ttcattttgt ggaggaactt ctatgcgaac caacatcaac caaatcgcac caacacaact
    28621 gaataacttt tactttagct actttagata ggtttgtatt catcatgatg gatacaaacg
    28681 ttaaacgaca gtttgtatct atgatggcta aagaactttg tattgcagca aaaagccacg
    28741 tagacaaaat gctaagtggc tgagcagtga aaatacaaag taaaagtatc aagcattcta
    28801 ctttttcaat ttattttccg acttttcaat ccatttggac attttgtctg aatggatttt
    28861 ttgtttccca aaattaagga ggaaaaagaa atgaaaaaaa atataatcat cggattaggc
    28921 gctgccctgc tactagcttt aggaggttgc gcacaaaaag acaattccgc agaggcatca
    28981 gatggacaaa aaacaattgg cgtattgcaa cttgtacaac ataactcgtt agatgctgcc
    29041 tataaaggat tcaaagaagg attagcagaa gaagggtata aagaaggcga taatctaaca
    29101 atcgattatc aaaatgccca aaacaatcaa gacaacctga aaagtatgag tgagaaactc
    29161 gtcaaagaag atcctgattt gctacttgga atcgccactc cagcagcaca atctttatta
    29221 aatgaaacaa ccactatccc gatgactgtc acagcggtca cagacttgga acaagcccat
    29281 ttagtcacat ccaatgaaaa acctggcggc aatgtgactg gcacgacaga tatggtgcca
    29341 atcgaaaaac aaatcggctt actgttaaat atcgtgccag acgccaaaac aatcggcatc
    29401 atgtataaca acggtgaagc aaactctaag atccaagctg atttggcaga aaaagaattg
    29461 aaaaaagcgg gcgtcaaagt caaagtactg acagcaaatt ccacaaatga tgtccaacaa
    29521 gtgacgaaaa gtctagctaa agatgtagat ggaatctata ttcctactga taacacgttc
    29581 gcaagtgcgg caagtgttgt cggtgaagtc gcaaaagaaa caaaaacacc tgtcgttgct
    29641 ggcgcagttg atcaagtcaa agacggcggt ctagcaacag taggaatcga ctacgaaagc
    29701 ttgggcaaac aaacagggat catggcagca aaaatcttag acggcaagaa tccaagtgaa
    29761 cttcctgtcg aaacagcgaa tgatttggaa ctgtatgtca ataaagacat ggccaaagca
    29821 ttaggcatca atccagacag catcaaagaa gctgaataaa acacaggagg attctcatga
    29881 caaatttgct ctctcttttc ttatcatctg catcgcaagg ggtcttatgg gccctacttg
    29941 cgattggtgt atacgtcact ttccgtatct tggacattgc cgacttgact gctgaaggaa
    30001 gctttccttt aggtgcaggg atcgcggcag taagtattac aaatggctat catccattga
    30061 cagcttgtct tctaggcttt ttagggggtg cggcagcagg gcttgtttcc ggactgcttc
    30121 acacaaaatt gaaaattccc gcccttcttg cagggatcat caccatgact ggtttatatt
    30181 ctgtcacatc ccgtgtgatg ggtgcgccga acatctcttt attgggtcag ccaacgatct
    30241 ttcattgggt cgagtcattc ggcataacaa aagacaacgc tgtactgatc gtcggtctgc
    30301 tttttgcttt gatcgttgta tgtttacttg ttttgttctt acgtacagaa acaggacttg
    30361 ctattcgtgc aactggcgat aatctggcaa tgagcgaagc aaacgggatc aacacagata
    30421 atatgaagat catcggttat atgatttcta atggatgcat tgctctttct ggctccttac
    30481 ttgcgcaaaa caacggcttt gctgatttaa attctggtat tggaacgatc gtaattggac
    30541 ttgcttctat tatcatcgca gaagttttgt tacgtaacca gccactattt ctccgtctct
    30601 tgactgtcgt tttaggtgcg gtcatttatc gttttatttt agcgttagtc ttcgaactga
    30661 atgttgaacc aagtgattcg aaacttgctt ctgctttcgt gctagtgatc tgtctttctt
    30721 tcccgcagat ccagcaaaaa ttccatttac ctcaaaaaaa gaaaggcggt agtgtcaaat
    30781 gaatgacgtc ttaacattgg aaaatctcca tcataccttt gaaaaaggaa cgatcaacga
    30841 aaatcatgtc ctgaaaggca ttaatctttc cttagatccc ggcgacttcg tgacgatcat
    30901 cggtggaaat ggggctggta aatctacttt gctgaacagc atcgccggta cattttctgt
    30961 cgatcagggt caaattttat tgaatggaaa aaatatcacg aaaaaatctg ttgtcgaacg
    31021 ttcgaaatcc atcagtcgcg tattccaaga tcctaaacta gggacagccg ttcgtttaac
    31081 ggtagaagaa aatcttgctc ttgctatgaa acgaggaaaa aaacgcggct tcttccgtgg
    31141 tgtcaaacca caagaccgaa catttttcaa agaccagctg gttcggctaa atttggggtt
    31201 ggaaaatcgt ttatcctcag aaatcggttt gctgtctgga ggacaacgtc aagctattac
    31261 gttattgatg gccatattac agcgtccaga actgatctta ttagatgaac acactgcagc
    31321 gcttgaccct gctacttcca aaacggtgat ggaattgaca gaaaagctga tcactgagca
    31381 gaatctcact gcttttatgg tcactcatga catggaagac gcgatccgct atgggagtcg
    31441 actgatcatg ctgcatcaag gaaggatcgt cgtagatatc cagcaagagg aaaaacagca
    31501 gctcacagtg ccccaattat tagcgttgtt caaacaaaac agcggcacgg cattaaaaga
    31561 cgatcaagta cttttatctt cataatcaga aagaggatgg gacaaaaaat gaacgcccac
    31621 cctcttcttt tttctctttc cttctttcgt ttttgcttta tgtatcaacc gttgtttctt
    31681 cgaaaactat tttgttctct ccttcttcct gatagaatgg atgggaaagg agcttgatgc
    31741 catgacaacg aaagacaagg tacttcaatt attgaaaaag acaccagatt ttctttccgg
    31801 agaaaagttg gcacagcaac tggaagtttc ccggacaagt atttggaaag ccatcaaaga
    31861 attggaaaag gcaggctatc gttttgagca cgggcctaca gggtatcgtt acctgccctc
    31921 tgacgtacta gatgcaaaag aaatccagca agagctacgg tcgtttattc ctgatttgaa
    31981 agtacaaacg atgttgactt ctgaatctac gatgaaagat gccaaactgg cagcagctca
    32041 aggagattct gtttcgaagt tatttgttgc tgatacacaa gaacaagctc gtggacgttt
    32101 tggacggcct tttttcgctg aacctggacg aggaatctac atgagtctct tgctccatcc
    32161 agacaaacgg tttgatgaat tgcctcaata tacgatcatc gcagccagtg cctgtgtgaa
    32221 agccattgaa tctgtaaccg gtcgttctgc ttcgatcaaa tgggtcaatg atatctacat
    32281 ggatggaaaa aaagtctgtg ggattttatc tgaagcagtc agtgatatgg aaagcggcag
    32341 gatcagtcat atcatcattg ggatcgggct taatttttct gttcctcaaa atcattttcc
    32401 agctgatctc caagaaaaag ccatctctgt ctttcccgac ggacaagcta gtgtcacaag
    32461 gaatcagcta atcagcaaaa tctggcaaga attgttcact tatctttctc acccagaaaa
    32521 agcgttggac ttctataaag aaaaatcatt cgtattggga aaaaccgtgt cgttcaatca
    32581 aaaaggaacg acttatgtcg gtcttgcaaa agaaatcggt gatcatggcg aactgatcgt
    32641 ccaaacaaat gaacagaaga tgagtttgtc ttctggcgaa atcagtctca catctatccg
    32701 tacgcaataa ttatcagatt tcacaaaagg acacctgatc tcatacagtg tccttttttc
    32761 tgaattctct tcaaaagact ctccttattc tatcagctga cagtttctct atcctctaga
    32821 aaaaggtatt ctaaaaatat ctataaagca tggtacactt tctttaaatg aatcaatgca
    32881 aaaaacaaga gagtcatcag tttacacgat caaaagagat agcaggccgc ttcctttttc
    32941 aatcttatag atagaccatg ggtaacgata aaaacaaaaa agaacttgcg acttattttg
    33001 ccgtaagttc ttttttagct agtgccttaa tctcttaatc ccaacaaacg tctcgcatag
    33061 atcacctgac cagagtgcat agcagcatca tcaataatag aaacgagacg cattcctcgt
    33121 gtgactggtg gtgtccagtt ctcgtcaatt acttcatcta gctcggaagg ttccagcctt
    33181 cttaagtaag aaatagcaaa atcagcagct cctcgcaagt atcctagcag atcttctatt
    33241 tgattagctt ggatctgctt tgcttgttcc agactatgct tccaatcttg ttcgttatct
    33301 gctatcgtca tcggaaaccg gctttcccag tcttgactat gccaaattgg agaagttcct
    33361 gctaaatcag caatctggaa atctaattct cgagcggtat gccaagccag ccaagtcatt
    33421 gatttgatct gcggggctct atcttccact ggaaaacgat tggcctcctc aatagagacg
    33481 ccttcaaaca aacgttcaaa ccgatcgtat gcccgatcca cactctcgca tagcatatct
    33541 aaataatcca tttattttcc tcctttttat ttatcctact tactttttcc taattctagg
    33601 atacctactt atatgttact acacaccctt attttttaca ctttaacagt caccatcatc
    33661 aatagtttgg agattgaaag aaaaagatag tgtcgaacta tcaattgaac aagaagcaac
    33721 aatatgaacg tttcattaat attctttaaa taaacaatga tttttcaaac gaacagttta
    33781 agactaggag aaaaagagta taataggaaa atgtttgaaa ggaaaggaag gggaaaaatg
    33841 aaaaaaaggg caagtttact agtcctagtt gctttgttgt tcaatatctt ttcgtatggt
    33901 gtggccgttt cagcggaaac atacattcct aaagaccctg ggaatattta taatgatcta
    33961 actggtaact atggcttgtt aggaattgct agtcaattcc acgtatttgc taaaaataag
    34021 acaactgtga atgcacatac agacggaaat atagcaacac atgaacttga tgcaaaaaat
    34081 aattttggta cagagcttca tgaaggttta ttgaaccaag aaataaatta tatacaaaaa
    34141 actgattctt tcgctaattc ttcaggcata cctacaggta gcacaagaat gaataaattt
    34201 gtcgttggca gtaacgtgga tgtagcactt caagatggtc atcctacagt caatggatcc
    34261 aggcttgatc acttaactcc agatgatttt tataaagatc gtgctgggca tgaatatatt
    34321 gatttcgagc aggaatttgc taaattgaat gtagcagcaa atgatcttgc aacgatcact
    34381 ccagctaaaa catatactgc tgcagatttt ccagacatga acaatcggac aatcgattta
    34441 actgatttat cagataccgg gtttttattg gtaaatatcg atgcagaagt gttaacgatg
    34501 aatacgccac ttcaaatcat taatccaaat gatcaagtag ttgtatttaa tgtcatcaac
    34561 agttcttctg cattgaacgt acagtcacca atcaaataca atgatcgttc aaatcatgaa
    34621 acagaagatt tttcggatgc gaatatctct tggaattttg gaaatgagat gactgatttg
    34681 acaatcagtg ctccttttca aggaacaatc cttgcgccta atgctactat ccgcattact
    34741 caaaaccaag acggaacgat tataggaaca aatgtgattt tagatgcagc taccaatcgt
    34801 tgggatccta acgaaatatt tatcagcgac aatggtacag atacgacaga cacaagcgac
    34861 agtacagata catctaccac aagtgatagc tcagattcat caacttcaag cgacagtaca
    34921 gatactacct ctaccacaag tgatagcacg gatacatcag cttcaagcga cagtacagat
    34981 actacctcta ccacaagt
//
LOCUS       DAPCMT010000014        33864 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPCMT010000014 DAPCMT010000000
VERSION     DAPCMT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.014"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            157..2055
                     /locus_tag="RD743_000555"
     CDS             157..2055
                     /locus_tag="RD743_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDU0562413.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2354..3073)
                     /locus_tag="RD743_000556"
     CDS             complement(2354..3073)
                     /locus_tag="RD743_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDU0562414.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3410..3742
                     /locus_tag="RD743_000557"
     CDS             3410..3742
                     /locus_tag="RD743_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562415.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3729..5732
                     /locus_tag="RD743_000558"
     CDS             3729..5732
                     /locus_tag="RD743_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDU0562416.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5751..6221
                     /locus_tag="RD743_000559"
     CDS             5751..6221
                     /locus_tag="RD743_000559"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDU0562417.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6242..6826
                     /locus_tag="RD743_000560"
     CDS             6242..6826
                     /locus_tag="RD743_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDU0562418.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6841..7827
                     /locus_tag="RD743_000561"
     CDS             6841..7827
                     /locus_tag="RD743_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDU0562419.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7817..8128
                     /locus_tag="RD743_000562"
     CDS             7817..8128
                     /locus_tag="RD743_000562"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDU0562420.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8150..9931
                     /locus_tag="RD743_000563"
     CDS             8150..9931
                     /locus_tag="RD743_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDU0562421.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9924..11300
                     /locus_tag="RD743_000564"
     CDS             9924..11300
                     /locus_tag="RD743_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDU0562422.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11307..11942
                     /locus_tag="RD743_000565"
     CDS             11307..11942
                     /locus_tag="RD743_000565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDU0562423.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11972..13327
                     /locus_tag="RD743_000566"
     CDS             11972..13327
                     /locus_tag="RD743_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDU0562424.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13519..14913)
                     /locus_tag="RD743_000567"
     CDS             complement(13519..14913)
                     /locus_tag="RD743_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDU0562425.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            15005..15976
                     /locus_tag="RD743_000568"
     CDS             15005..15976
                     /locus_tag="RD743_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDU0562426.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15973..16755
                     /locus_tag="RD743_000569"
     CDS             15973..16755
                     /locus_tag="RD743_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDU0562427.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16924..17295)
                     /locus_tag="RD743_000570"
     CDS             complement(16924..17295)
                     /locus_tag="RD743_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0562428.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17429..17872
                     /locus_tag="RD743_000571"
     CDS             17429..17872
                     /locus_tag="RD743_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDU0562429.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17915..18724)
                     /gene="yidA"
                     /locus_tag="RD743_000572"
     CDS             complement(17915..18724)
                     /gene="yidA"
                     /locus_tag="RD743_000572"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDU0562430.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18743..20110)
                     /locus_tag="RD743_000573"
     CDS             complement(18743..20110)
                     /locus_tag="RD743_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDU0562431.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(20094..20924)
                     /locus_tag="RD743_000574"
     CDS             complement(20094..20924)
                     /locus_tag="RD743_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDU0562432.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            21087..21524
                     /locus_tag="RD743_000575"
     CDS             21087..21524
                     /locus_tag="RD743_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDU0562433.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21600..22220
                     /locus_tag="RD743_000576"
     CDS             21600..22220
                     /locus_tag="RD743_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDU0562434.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22409..22915
                     /locus_tag="RD743_000577"
     CDS             22409..22915
                     /locus_tag="RD743_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDU0562435.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22932..23831
                     /locus_tag="RD743_000578"
     CDS             22932..23831
                     /locus_tag="RD743_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDU0562436.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23918..24802
                     /locus_tag="RD743_000579"
     CDS             23918..24802
                     /locus_tag="RD743_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDU0562437.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24994..25065)
                     /locus_tag="RD743_000580"
     tRNA            complement(24994..25065)
                     /locus_tag="RD743_000580"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25030..25032),aa:Arg,seq:ccg)
     gene            25125..26459
                     /gene="rpoN"
                     /locus_tag="RD743_000581"
     CDS             25125..26459
                     /gene="rpoN"
                     /locus_tag="RD743_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDU0562438.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26608..27699
                     /locus_tag="RD743_000582"
     CDS             26608..27699
                     /locus_tag="RD743_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDU0562439.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            27740..28741
                     /gene="gap"
                     /locus_tag="RD743_000583"
     CDS             27740..28741
                     /gene="gap"
                     /locus_tag="RD743_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDU0562440.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28851..30041
                     /locus_tag="RD743_000584"
     CDS             28851..30041
                     /locus_tag="RD743_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDU0562441.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30145..30900
                     /locus_tag="RD743_000585"
     CDS             30145..30900
                     /locus_tag="RD743_000585"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDU0562442.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            31016..32314
                     /gene="eno"
                     /locus_tag="RD743_000586"
     CDS             31016..32314
                     /gene="eno"
                     /locus_tag="RD743_000586"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDU0562443.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            32713..33852
                     /locus_tag="RD743_000587"
     CDS             32713..33852
                     /locus_tag="RD743_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDU0562444.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        11186 a         5996 c         7210 g         9472 t
ORIGIN      
        1 gaaaaatata gaaggaagct ggacatttat tgttcccagc ttcttttttg tatagaccta
       61 tttctatttt tttattatat tggatatata tattcattga aaatagagca taacaacttt
      121 ataatgttga gtataccaat ttttggaggc gttttaatgg accgctattt agatttactt
      181 tcagagaagt tccctacaac ggaagatgtc atcacggaaa tcatcaatct cgaagcaatt
      241 ttgcagcttc caaaaggaac agagttattt ttgagcgata tacatggcga atttcctgct
      301 tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa agaccttttc
      361 tctgaacaaa tgacggaaac ggagatcagc caattcacta tttttacggc ttatccagaa
      421 tatgtgttga cgactgattg gtacaaaaaa caagataaat cccagctgat ccatcgtttg
      481 atcgatcttt tacggtttac gacagtcaaa tacactcgat caaaagttcg aaaaggattg
      541 ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgatcg tatcaacggg
      601 aaaaaagact atgtaaaaaa aatcattgaa caacttgtct tgatgaagga agaagaaaaa
      661 tttttaacaa aacttgcgca gacgatccaa aaatctgtca tcgatcattt gcatatcgtg
      721 ggtgatattt ttgatcgagg aactcaagca gccaaagtaa tggatcgaat catggatttt
      781 tcctctgtag atatccagtg ggggaatcat gatattttgt ggattggtgc gtattgcggc
      841 tctgaggcat gtttattgac gcttttacgg attgccgcac gctacaacta tttatttgaa
      901 ttagagaaag aatatggatt aaatttacgt ccattatttt cttttgccca tcaaacctat
      961 cagaagaatc cagtatttac ccctaaaaac aggaaagatc tatctgaaag agagcaagaa
     1021 gaattagaaa agatccatca agcactttct atcctccaat tcaaatcaga acatcagttg
     1081 ttgaatcgac gacctgaatt taaaatggac gatcgtaaat tgttggaaaa aatcaactat
     1141 gaggaatcaa cgatcgtttt agaaggtcag ctctatgagt tgaaagggac ttgttttcaa
     1201 acgatccagc cagatgatcc taaaaaattc ttggaagagg aacaaaaagt gatcgatagt
     1261 ttgatgtatt cattccagca ctcgttaaga atgcaaaaac acatgacgtt tttgatcgaa
     1321 aaagggggaa tgtatctcac gaacaatcag catgtactgt tccatggctg tatacctgta
     1381 gatgaaaagg ggcagctctt agcatttgaa ttagaccaat cttatcgcgg aaaagaactg
     1441 ctgggatttt ttgaaaaaca gataactgaa agcgcaaaag atttgactgc aaatgaagat
     1501 ttggcgaccg atttgatttg gtatgcgtgg agcgggccat tttcgccttt attcggcaag
     1561 agtaaaatgg caacttttga gcgctatttt ctcacagagc ctcatacgca cgtcgaaaaa
     1621 agcaatccct attatcactt gcgagatgaa gagtggttca gtgaaaaaat tttggcagaa
     1681 ttcgatgcaa aagaagaagg ttcagcaatc atcaatggac acacgccagt aaaagtaaaa
     1741 aaaggagagt caccgatcaa agcaaacgga aaagcatttg ttattgacgg aggtctatcc
     1801 aaagcttatc aaaaaacaac cgggattgct ggctactcat tactatgcaa ttcttacggt
     1861 ttccaaatcg tgacacatta tccgttcttg cctattgaac aacaatttgc aaaaaaatct
     1921 gatcaaacga atgtaaaaaa agtgatcgag cagcagctcc cacgcaaact taatcggaat
     1981 acgacaaaag gaatggaatt acagcaacag attactcaac tccaaaggct gttggattat
     2041 cgaaaggacg attgagaaaa atgctagcca acattgctca atagactaaa aagcgactgc
     2101 aacaaaaggc gtgtcgcagt ctccttcgga attagctcaa aaaacggtaa aatattagaa
     2161 gctatttttc tgtttatttc tctaatactc aggtcagggc gctgatttca cgacctcttc
     2221 cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca acaaacatga
     2281 gaagctgttt tttgaaattg ctccttgtga ctcagagttg aacggcaggc tcacgaccta
     2341 atcagaaaaa cgattaaaat tcattcacaa agaaatcgaa taaacgctgt tcagatgaat
     2401 ctacacgatg agaaagttcc ggatgccatt ggacaccaag gattttgtgc atattatctt
     2461 tactttggac tgcttcaatg agtccatcgt tggaaaaggc gatggctttt aacaaaggag
     2521 aaagatcttt gactgcctga tgatgatagg aattcacaaa gtatgaatca gggagaaggt
     2581 cacttaataa actgtccgaa acgattttga cttcgtgagt tgcaaactgc ggggcagtgg
     2641 gctgttgttc gtgttttacg gaccattttg gatataatga aagatcttga taaagtgttc
     2701 cctccaacac gacattcaat agttgaagtc ctcggcagac tgcaaaaatc ggttttcttt
     2761 gtttcaatgc ttccaagatc aatgcctgtt cgaattggtc tcgttgaata tttgtttccg
     2821 tcagttttgg atgagggtct tccatgtaaa gttgcggact aacatcctga ccgcctgcaa
     2881 gaatgagttt gtcgattttc tcaattagtt gaggcgctga atctggagac atgactggca
     2941 gaataagagg taatccttga gcatctaaaa ctgcatccac aaatccctgc ggtgtatagc
     3001 ttacttgatt tccttggaaa gtatctgtcg ctcgaatcag ttgattcccg gcaatgccga
     3061 tgattggttt catttttatt ccccctaaag tagactaatt gtaacaagga tttaaaaaaa
     3121 tttaaactat gtaagacttg cccctatact tatctgttct tgctgtgtta ctatctgtaa
     3181 agagaatttt cgtgtgaaat tcatgacgat agttgaaaat attgcttaat ttgttataat
     3241 aaaaagtaag cagaaataaa aaggttgggc gtaagcttca aggatctagt tcttcaaggg
     3301 gtgagaagga cattttattg aaggggcctg cctatactaa gttatcttta gaggtacata
     3361 ctttcaatga aaaaagaaag catgtacctc ttttttaaat cgaaaggaag tgaagcaagt
     3421 gttagaagct ttggaacaaa tccgaaaagc agaagaagaa aatgagaagc gtgctcgaac
     3481 cctacggcag cagttgaaga cctatgcaga agaaaaagag caagaattgg agataatcca
     3541 aacgaacagt cggcaacagc tgaaacagtt ggtagaagaa aaagaacaat tcgaaaagca
     3601 agaattagaa aaagaaaaaa tgaaattgaa agcagaagca gacaaggtgc aagcaacttt
     3661 gaaagaccaa tatcaaaagc atcagagtca agcaatcggt gcaatcattg aaagggtgaa
     3721 agaaacttat ggccgtcact aagatggaaa aagtgaccct catttcagat aaaagaaatc
     3781 aggagaggat tttacaagca gtacaaggaa tccaaaatgt tgaaatccgg gatctgtatc
     3841 aaaatagaac aaacaaccag tgggtagaaa aatattttcc agataccacg attattgata
     3901 aagaagcaaa attaagcgct ttaggcaatc ggctgaaaga catccaagaa gctatccaat
     3961 tcatacaaca ccacggcgat agccgtcaaa agaaactcca tttgaaacgt caagaacttt
     4021 ccttacaaga cttggaagca aattattccg aagaaaattt catgaataaa ttagaagaag
     4081 tccttgattt aaaagaaaag tgggaacagc taactgaaaa acgtgatcgt ttagaagaag
     4141 aggaagaatg gcttcttcat tggcaacagc tggatattgc tccaagtatg tatgcaagta
     4201 agacgaccat ttttgagatg gggactgtga gtgcagcaaa ttttgaaagt ttcaaggaag
     4261 aactaagtaa gcttgatgac gtttatatgg aagaaataga ttatacgcca aaacaagttt
     4321 atctttcgta tgttgcttta aaaaaatcag ttgctgctat agaagaaatt gctgggaaat
     4381 acgggttcaa taaagaaacg tatccttatg aacaaaatcc taaagaaaaa ttgcaagaaa
     4441 ccaaacagca attatccatt gttcacgagc agcaaaaaaa gataacgaca gcgttaggtc
     4501 agtgcagcgg ctatatccaa gaatttgaat gggtagaaga agtcacactt gccgttgcag
     4561 aacgggaaaa agtaaaagaa cggtttatcc atgccactta tctgatcgtt ttgcaaggtt
     4621 gggtagatgc agaagaaaaa cagaatttga tgcatgtgtt aaacgaaact gtatcagaag
     4681 aagatatata cgttagtttc gaaacaccaa actcagaaga gattcagcag gaagtcccta
     4741 ctaaattgaa aaaccatccg cttatcgaac cttttgaatt gctgactgaa atgtacagtc
     4801 tgccaaaata tgaagaagta gatccaacgc cttggatgac accattttat cttgtcttct
     4861 tcgggatgat ggtcgcagat gttggttacg gattgctgat gctcattggg acaatactag
     4921 ctcaaaaact atttgtttta ccccgcggga tgaaacgttt cgtaaaattc tttgagattc
     4981 tctcggtacc atcgatcatc tggggattga tctacagttc atttttcgga caggcattac
     5041 caaaagaact atttggtatc cgcttgcctt tcccaatcct atctacgacg gatgacgtca
     5101 atacgatttt gattttatcc gtgattttcg gtctgataca gatcttggtt ggattattcg
     5161 tggcagcaaa ggaaaatatc aggaggaaag attacttgag tgctgtcagt gatggttttg
     5221 cctggcaagg gatcctgatt gggatcgtta ttgcattgat tggtgtcatg ttgataaaaa
     5281 atacagcatt tgtttatctt ggcgcggggc tatcgatcat tgctgcttta tgtatcttaa
     5341 tcgtgccgat tatccaatcg ccgtctaaag tcaagggtgc tgccaaagga gcgtataatc
     5401 tttacggatt gactggttat atcggtgatt tagtcagtta tacacgtttg atggcactag
     5461 gtatttcagg cggaagtatt ggcgcggcat tcaatatgct agttgctttc atgccgccag
     5521 ccgctcgatt cagtgtaggg atccttttga tcatcgcttt acacgcactg aatatgtttt
     5581 tgacactgtt aagtgcctat gtccatggtg cacgattgca gtatgtcgaa ttttttggaa
     5641 aattttatac aggaggcgga cgtgcctttg acccgctgaa aacggcggaa aaatacgtca
     5701 acatcaatca taagaaaaaa aacaataaat aattggagga tttttataca atgatggatt
     5761 acttaattag tcaaaatggt ggaatggtat ttgcagtatt agcgatggca acagcgacga
     5821 ttttttcagg gattggttca gctaaaggtg ttggaatgac aggtgaggca gcagcagcgt
     5881 taacaacaag tcaacccgaa aaattcggac aggctttgat tctgcaattg cttccaggta
     5941 cgcaagggtt gtatggattc gttatcgcct ttttgatttt tatcaactta ggaaatgata
     6001 tgtcagttgt acaaggattg aactttctag gcgcttctct gccgattgct tttacaggtt
     6061 tgttttcagg tatcgcccaa gggaaagttg cagcagcagg tatccaaatt ttagcgaaaa
     6121 aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat ggttgaaaca tatgcgattt
     6181 taggtttcgt tatttccttc ctattagtat tgaatgccta agcataaagg aggaaaagca
     6241 gatgaacgcc attgaaaaaa tcatctctca gatgaatgaa gcagcagagc aagaaagagc
     6301 ggcgttagag caagaagagc gaatgaagat cgatcaaaac tttgaacaaa aacgtacgca
     6361 aatcgaaaca gaacatcaaa aacaaaaaga aaaacaaatc gaattacttg aaaaaaaata
     6421 ccgtcagctt aggaatcgcc aacaagtaga agtacgacaa gaaaatttga atgcgaagca
     6481 ggaattttta cgacgattgt ttgcagatgc aatagctgaa atggagaatt gggatgaatc
     6541 tgaacaaatc cagtttatca aaaattcatt gtattcatta cctttgacag gcaaagtaac
     6601 attcattgct ggagaaaaat cagcagctta tctttctcag acattgctgg atgaatggaa
     6661 caatgaactt ccctttatga tggttttaag tgatgaaact gttgctgatc aagcgggttt
     6721 tctgataaac gatcaaggag ttcagtataa ttttcttttc agcagccttg tacaagatat
     6781 ccaaggaacg atgagttttg agattgccaa tcaattattc gaataaggag ggatcagtgt
     6841 atggaatacc acgaactaaa tcccttgatt cgcggaagag agcttgaact tttgtccaat
     6901 gagacatttg aacggatgat tcaaacaaat tctgtcgaag cacttggtga aatattgaaa
     6961 tccacgattt acagtccata tatttatgac ggatatgaat atgacttcga acagaatctt
     7021 ttaaaggagc agagccgtct atttcgctgg ttaaaggaat cagcaccgga gcctgaaatc
     7081 gtttggatct atacgatgcg ctatactttc cataatctga aggttttgac aaaagccgag
     7141 atgacaaatc aaaatctgga ccatttgtat atcgacgatg gattttattc attagaaacc
     7201 ttgaaggatg ccattcatac gcaagtttca aatgaactcc ctgaaagcat tatggacagt
     7261 atacgagaag tacgtgatta ttttgaagag tctaccattc ttcagggaat tgacgtgatt
     7321 tatgatcgac agtttttgac agagcagcga cggcttgccg ataagctagg atatcaagaa
     7381 ttgctgacag aagtcattgc ttttattgac ttgaccaata tcacaacgat ggctcgagga
     7441 atattgcaga aaagaacata tgggttcatg acgacggtac tttccagctc gggaagtatc
     7501 gaaaaagaag tatttttgaa ctttgtagat aaagatatgg aagattacac gaattttcta
     7561 ttagagacag attatgctga ttttctgcgc cctgtcatcc gtgagggaaa tcttgatctc
     7621 gtaaagcttg aacagttgaa agatgattat ctaagttcat tgtatcaaga agcacaaacc
     7681 caggctttcg gaccactgcc actactagcc tttttgaacg caaaagaggt agaaagcaaa
     7741 aaccttcgtt tactggttat cggcaaaaga agccatttcg aaccagaaac aatcagaaag
     7801 cgggtgagac aagtctatga cgcataagat cggtgtagtt ggcgacaaag attctgtttt
     7861 gccattcaaa ttgtttggct ttgatgttcg ttatggtaca accaagcaaa ccgttcgtca
     7921 ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt tatgtcacag agcaatgtgc
     7981 agcattgagc ccagaaacga tcgaacgtta caaggaacaa atgatacctg ctatcgtatt
     8041 gattcccaat catcaaggat cattagggat agggctggaa gaaattcaaa aaaacgtaga
     8101 aaaagcagta ggtcaaaata tcttatagta tgaaaaggag atagaaattt tgcaaattgg
     8161 aaaaatcata aaagtctccg gacctcttgt aatggctgag aacatgtctg atgccagtat
     8221 ccaagacatg tgtctagtgg gagatctagg agttatcgga gaaattatcg agatgcgtgg
     8281 cgatgtggcg tctattcaag tatatgaaga gacttctgga attggtccag gcgaaccggt
     8341 tcgctctact ggtgaagctt tatctgttga attaggacca gggatcattt cacaaatgtt
     8401 tgacggaatc caacggccat tagatacctt tatggaaatc acacaaagta atttcttagg
     8461 gcgcggagtt caattgcccg cattggacca tgagaaaaaa tggtggttcg aaccaacagt
     8521 agaagccgga gaaacggtga gtgctggaga tgttatcggg atcgtagaag aaacaaaagt
     8581 tataaaacat aaaatcatgg tgccaaatgg gataaaagga acgatcaaaa caatcgaaaa
     8641 aggttcattc actattgatg aaacagtatg tgtcattgaa accgaaaatg gtgacaaaaa
     8701 tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg ccaatcaaag aaaaattaaa
     8761 cccggatgca cccatgatca ctggacagcg agtgattgat acattcttcc cagtgacaaa
     8821 aggaggagca gcagctgtac ctggtccttt tggtgcaggg aaaacagtcg ttcagcatca
     8881 aattgcaaaa tgggccgatg tggacatggt tgtctatgtg ggatgtggcg agcgtggaaa
     8941 tgagatgacg gatgttttaa atgaattccc tgaattgatt gaccctaata caggagaatc
     9001 gttgatggaa cgtacggtat tgattgctaa tacatcaaac atgccagtag cagctcgaga
     9061 agcttctatc tatacgggaa tcacaattgc ggaatatttc cgtgacatgg gttatgacgt
     9121 tgctatcatg gcagactcca cttcacgatg ggcagaagca cttcgtgaga tgagcggacg
     9181 tttggaagaa atgcctggtg atgaaggtta tccagcttat cttggttcac ggcttgcgga
     9241 atactatgaa cgatctggaa gagtcattgc attaggatca gaaggtcgcg aaggaagtat
     9301 aaccgcaatc agtgctgtat ccccgtctgg aggggatatt tcggaaccag tcacacaaaa
     9361 tacattgcgg gtcgtaaaag tattctgggg tcttgattcc agtttagccc aaaaaagaca
     9421 ttttccttcg atcgattgga tacaaagcta ttctctttat tcaacagaag tagggaagta
     9481 tatggatcat atccttcaag ctgactgggc atccatggtc accgagggca tgcgtatttt
     9541 gcaggaagaa cagcagctga atgaaatcgt gcgtttggta gggattgatt ccctttcaga
     9601 taatgaccgt ctgacattag aagtggcaaa atccattcgt gaagattatt tgcagcaaaa
     9661 tgcttttgat gatgtcgata cattcacttc aagagagaaa caattcaaaa tgttgaagat
     9721 gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg ttaggtgctt atttaagtga
     9781 gatcatggac ggtaccgtat cgattcgtga aaggatcgga cgaagcaaat atatcccgga
     9841 aacagaatta gaaaaactag atcaaattat tggtgaaatc aagcaaacga tgcaaacgat
     9901 cgtttcagaa ggaggcatga gcaatgatta aagaatatcg tacgatcggt gaagtcgtcg
     9961 gtccattgat ggcggttgaa aaagttgctg gtgtcaaata tgaagaatta atcgaagtcc
    10021 gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga agtccaagaa gataaagcaa
    10081 tggtccaaat attcgaagga acaagtggta tcaatttacg tgactcttct gtacgatttt
    10141 taggccatcc tttagaacta ggtgtatcgg aagacatgat cggtcgagta ttcgatggtt
    10201 tgggacgacc gaaagataac ggaccagaga tcctgcctga aaagcatttg gatattaacg
    10261 gagaagtaat caatccagta gctcgggatt atccagatga gtttatccaa acaggtatct
    10321 ctgcaatcga tcatttgaat acattagttc gtgggcaaaa attaccagta ttttctggct
    10381 ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg acaagcaacg gtactcgatt
    10441 catcagatga tttcgccgta gtctttgctg ctattggaat cacttttgaa gaagctgaat
    10501 ttttcatgga agattttcgt caaacgggcg caatcgaccg ttctgtcatg tttatgaatt
    10561 tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg tatggctttg actgctgcag
    10621 aatatcttgc ttatgaaaag ggaatgcatg ttttggttat catgacagac atgaccaact
    10681 atgcagaggc attgcgagag atttctgcag cacggcgaga agttccagga agaagaggat
    10741 atcctgggta tctttacacg aacttagcta ctttattcga acgagcagga agaatccgcg
    10801 gactaaaagg atcggttact caaattccga ttctaacaat gccagaagac gataaaactc
    10861 atccaatccc tgacttgact ggttatatta cagaaggaca gatcatcttg actagagagc
    10921 tttacaagag tgggattcag ccaccaattg atgtactccc gtctctttcg cggttaaaag
    10981 ataagggaac tggacccgga aagacaagag aagaccatgc agctacgatg aatcagcttt
    11041 ttgcagccta tgcacaagga aaacaggcaa aagaattagc cgtagtttta ggtgaatctg
    11101 ctttatctga tgtagacaag atctatgcga agtttgctga gcggtttgaa aaagaatatg
    11161 tcaatcaagg attctacacg aaccgttcga ttactgaaac acttgatcta gggtgggaac
    11221 tgctatcgat gttgccaaga accgaattaa aacgaatcaa agacgacatg ctggacaaat
    11281 atctgccaga agggaagtga gttctgatgg cacgattgaa tgtgaatccg acccgcatgg
    11341 aattgactcg attgaaaaaa cagttgacaa ctgccacaag agggcataaa ttattaaaag
    11401 ataagcaaga tgaactgatg cgtcaattca tcctgttgat tcgaaagaac aaccagctgc
    11461 gtcaagaaat ggaagagtct atgacagaag caatgtctga ttttgtattg gcaaatgctt
    11521 cgatcacgga acctttcatc gaagaattat ttgttttgcc ggcggaaaac gttgatttgt
    11581 ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt gatgaatttt gaatatgatg
    11641 aacagctgac agacacacct ttggaatacg ggtatttgaa ttccagtgcc gaactggatc
    11701 ggtcaatcga tcggtttact accctgcttc caaaactttt ggaactgaca gaaatcgaaa
    11761 aaacttgtca actgatggcg agtgaaatcg aaaagacacg tcggcgagtc aatgcattag
    11821 aatatatgac gatccctcaa ttagaagaaa cgatctatta tattcgtatg aagctggaag
    11881 aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa aaatatgggt accactgact
    11941 aagtggtatc tttttttaaa agaggaaaac gatgattaaa caaagcaaac aaataaagaa
    12001 acgtttgccg gctgttcagc ttctcgcatt aggatttttt ctcctgattt tagttggcgg
    12061 cggcctactt accctaccat tttttagtaa tagtgggcat gggacaaatt ttatcgatgc
    12121 gcttttcact gcaacttctg ctgtctgtgt aacgggattg acaacattga atacagcaga
    12181 gcattggaac agtgctggtc aatttttgat catgcttttg attgagatcg gcggtcttgg
    12241 ttttatgatg gttccgattc tatgctttgc attgataaag aagaaagtca gtctcagcac
    12301 acgtattata ttaaaagaag cgctgaatct agaagaagta tcaggtgtga tgaagctcat
    12361 gctttacatc ctaaaattgg cctttgttat ccaattgatt ggtacggttg ctttatcttt
    12421 tgtatttgtg cctgaatttg gctggttgaa aggtatctgg tacagtttgt tccatgcaat
    12481 ctctagtttt tgtaatgcag ggtttgattt gttaggagac agtttagcca atcaccagca
    12541 aaatgtttat ttgatcatgg tggtatctgc tttgatcatt tctggtgggt taggcttcat
    12601 cgtatggcgt gatttactgg aatatcatca gaaaaagaag atgacgattc attcaaaaat
    12661 cgcattaacc gttacaggta cccttttatt aggcggattc gttgtgttct ttttcacaga
    12721 gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa cgattggcaa acaccttttt
    12781 tatgagtgtg acacctagaa cggctggata ttattcagtt gattacctcc atatgagcca
    12841 tgcaggtctg attttaacga tgtttttgat gtacatcggc ggaacatctg ggtcgacggc
    12901 cggcggattg aaaacaacta cgttaggtgt gttgctgatc cagatgcgtt cgatgttgaa
    12961 aggacgtacg agagcggaag catttggccg taccatccga caaggcgcag tgttgcgggc
    13021 acttacttta ttttttgtta cgttgacgtt gtgtgtagtc gccatcatga ttttatcggt
    13081 gactgaaacg atacctaaaa cttctggcat cgaatatatt gcttttgagg tcttctctgc
    13141 gtttggtaca gtaggtctta cgatgggatt gacgccagac ttgactgcaa tagggaaatt
    13201 gatcattata gcgcttatgt acattggacg agttggtgta ttgactgtgg tcttctctct
    13261 gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat ccagaagaaa gtgtcatgat
    13321 tggataattt catcttctga cagaaaccta gtgatttagt aaaataagaa aataaaataa
    13381 tttttcttgc ttcttggata ctttaagtgc aaaatgttcg tttatttttt gaggcactac
    13441 ccgttgttga acctcgggta gtgcctcttt tttattgaaa ggaatcaata agatttgatt
    13501 cctttattta tcaataggtt ataattcata tttttttatt ttttctttta cgatacttag
    13561 tccagaagct aaaatttcaa ggttattaga ggatagggaa attcgaacga atgcatctgg
    13621 ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg ataccgttag caagaaagtc
    13681 attctcaatt atcttagggt caatctcagg atcaactgga agaattcgat agtaagggac
    13741 acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa agctgattgg ctctttttgt
    13801 aagacttaat ttagtttcta agatcctatt tgcttcattt gaatggagga tttgagtgat
    13861 gacttccaca tctaatccag aagttttcac atttatatta aaaattgctt gttctatctt
    13921 gctttggaca tgtactggaa ctacaagata agcaactcgc aatccagaac aaataaattt
    13981 tgtcattcca gctaagtaga ttgtttggtt aggtaataaa ttttggaaag gaggaatgat
    14041 cgtatgttga tagtaagttg ttaaaaatga atggatatca tcttcaataa cccatagacc
    14101 ttctttttca atcacttcgg ctaattgctc tcgtcttttg tgagagattt gaaaccctat
    14161 aggattatta caagaaggca taagaaagac accatgaatc ttcttttttc tacattcctg
    14221 aatcaatata tcaggtcgca ttccttgctc gtcaaaatca attggaacga tctctaaatg
    14281 atataactga gccaattcaa tgaaattagc gtatgtatat cggtctgttg ctattctatc
    14341 acctggagag aaaagtgcag aaagtgtgat agccaatcca ttttgaacac cggaaacaat
    14401 agatagagat tctaaggacg gaatgactcc ttgagttttt aaccaatttg ctgcagtagt
    14461 caattgaaaa tttgtgcctt tgggatcttg gtaattaagc aaaccaacaa gatttgggtt
    14521 attactagtt gattgaataa acgggacaac gaatttatta cattcttcaa aagaactaac
    14581 cagaccaaaa tcaatgatat gttcggttac ttcattggta gagatggtga tggactcaaa
    14641 gacattggga gaaataaatg tgccacttcc aatattcgta tacagcaaac ctttctcaat
    14701 tccatattta taagccttgc caacggtagt aaaatttaaa tctaaataat cagctagctc
    14761 tctttgggag ggtaactgag tgtttttctg taatttattc tttaaaatat cttgttctaa
    14821 ttgttgaatc aacgatcgat aataaggacg cttcaatttt tgtttgtctg gtcgccaagt
    14881 taacgggtaa ttgtcataag aattaatagg cattttctcc actccttaaa attgtatacc
    14941 atacaatttt accattgatt gtattgattt tagaagttaa tcttttgtga ggagggaatt
    15001 gtctatgaaa attattaatt ttgaggaagt agtagcttct tttagttttg aagaagcgat
    15061 tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt aaaatcagcc aaaatcctcg
    15121 attggtggaa attttacctg acggaaaagg caaaaatatg tttgcgatga tgcctgctta
    15181 tttgggggag aacagatatt ttggcacaaa agtcattacc gcttttccgg ataatcatca
    15241 gtatcattta ccttctcatt taggagagat tttcttgttt gatgctcaaa acggactacc
    15301 caaagcattg ataaacgcaa atgcagtcac ctggattcga acagctgctg tatctgcgct
    15361 ggcaacagat tacttagcaa atccgcatgc agaaacactt gctttaattg gagctggtca
    15421 acaagcattg tcgcatttga ctgcgatttc taccattcgt cccatcacta cagtgtttgt
    15481 ttacgatatt ttagaagaaa gacgaaatac ttttatcgct aaagctaaaa aacagtatcc
    15541 taaaataacg tttataaatg caatatcagt agaagatgca gtacgtccag cggaaatcat
    15601 ttgtacgttg acctctagta aagaggcatt tcttcaaaaa gagtgggtaa gggaagacgc
    15661 acatatcaat gctgtgggca catttactcc tgatacaaga gagataagta catcgttgat
    15721 ggaaacttct caaatctatg tagatgatta ctcagctgcc ttaacagaaa gcggggacct
    15781 gcttatagcc atggcagaag gagatttaga ggaatcttgt attgttggta cattaggcga
    15841 attagtttct gagaaaaaaa cattttataa aggagaatcg aggccgacga ttttcgacgc
    15901 agttggtcta gctattgaag atctttgttg tgcagaatat atttataaca aattagagaa
    15961 aggggcttta aaatgaactt agattattac gtagttgatg catttgcaga tgaggtgttc
    16021 aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc tgccagaagg tacgatgcaa
    16081 aaaattgcca ttgaaaacaa tttatctgaa acagcattta cagtgaagaa aaaccaagaa
    16141 tttgagctta gatggtttac gccagatcga gaaatcgatc tttgtggtca cgcgacatta
    16201 gcaacagcat ttgttttatt taactattac aaaatacctg atgaaacgat taaattttct
    16261 actcaaagcg gtaatctatt tgtgacaaga attaaagatt attattacat ggattttcct
    16321 agtattatgc cgaaaaaagt gccgatattg tctgaatatg aagaagcgat tggggctaag
    16381 attaaagaag cctacttagc acgtgattta tttttcgtat tagaagatga aaaaacggtg
    16441 gctaaattgc agccagattt tacagctatc aaaaattttg aattaggaat aggagtcatc
    16501 gtgacagcag aaagtgtgca aaaagacttt gtttctagaa ccttctttcc aaaattaaca
    16561 atcaatgaag atccagtatg tggatcagca cattctaatc ttattcctta ttgggcagaa
    16621 agaatgaata aaacaaaatt gtcagcttat caaatgtcat caaggggcgg ggatctagaa
    16681 tgtgaattaa aagaagatcg tgtgataata ggaggaaaag cagttttgtt ttctaaaggt
    16741 acctcgttta tttgatttct ttcaaatgaa ttaaaaataa attggtggag agtggcaacc
    16801 ttatagctac aatggttgtt aaaaaaagac gcaaactttt tcaatttaat gaaaagtttg
    16861 cgtctcattt taattattgg tgtttgtttt ttattcaatc aagagtaaaa tgccccatac
    16921 taactatcct cggatgactt cgattttata accatcaggg tcaatgacaa agtagtaaga
    16981 aggcgcagtg ccaggtaatc cttttaagtc agtaatctca agaccggcag ctttatgttg
    17041 ctcgtgtaat ttttctagat catcagctgc tatagcgata tgaccatagc cgtctcctag
    17101 atcatacgca ccatgatcat agttgtaagt caactctaat tcatagtcat ccccaggaag
    17161 tgtcaaataa acaagtgtaa atttgttttc tggaaaatca cggcggcggc tttcttcaaa
    17221 accaaatgcc ttttgataaa attcgattga tgcttcaaga tctttcactc gaacacatgt
    17281 gtgagccatt ttcatagatt catctctcct tttcctttta ctcaatcata acaaaaagaa
    17341 gtaaggaaat agttgatttg tgcttgttca tgagaaattt ctcatgtaaa atggatagaa
    17401 aataaaactg ttaaggtgga aactattcat ggataaacca atttttggtg aaaaaaagca
    17461 atttgctgat tatcaaaccc gttatgcagc atatattgtc atagaaagag atcagcagat
    17521 tgcagtaatc gaggctccaa atggtgcttt ttttcttcct ggcggggaaa ttgaaggaga
    17581 agagacgaaa gaagaagcga ttgtccgtga gctgatagaa gaaatgggaa ttgtggcaga
    17641 aatttcttct tatctgggag aagctgatga gtatttttac tcgaattatc gtgcaactta
    17701 ttattataat ccaggatatt tctttgttgc tgattcttgg aagcgtatag gagaaccaac
    17761 tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa gcgcttgtga aattgaaacg
    17821 tggtagccac cgatgggcag tcgagagatg gatcgaaaaa aataaaaact gaaagaaagc
    17881 gagtagatca gctttctctc agttttttta tttattacag tacatatttt ttgattgctt
    17941 cagctacacc gtgctcgtcg tttgatgaag tgatgacatt tgctgcttct ttgacaagag
    18001 aaacggcatt tcccattgct actcctagac cagcatattc aatcatggat aagtcgttct
    18061 cgttatctcc gatagccatg atttcatctt gatcaatacc aaggtgctgt gcgagattcg
    18121 caactgccgc acctttactt gcttctttat ttagtacttc gaagtaatag ggcgcacttt
    18181 tgaccgttgt gtatttatca cggaaagttt gcggtaaacg cgcaattgct gcatctaaaa
    18241 tttctggttc gtcgatcatc atcattttga cgatgttcat gtctggtgtc atttcttcag
    18301 gtgttcgata ttttagcggc atcttgacta aacttgcttc gtggatcgta taagcgctga
    18361 tatctcgatt aggcgtgtag atcgctttgt catcgatcga atggagatga ctgccgacac
    18421 ggcgtgccat cacttcgatc tctaggaaat cttcatgtgt cagtccgtga cgggcgatga
    18481 ttttacctga tttcgtttgt tgtacgagtg ccccgttata agtaataacg taatcattat
    18541 cttgaaaaag gttcaactcg tttaactgtt cctggacacc tggtaaggga cggccagtgc
    18601 ataaaacgat cgaaacacct tttgcaacag cagcggcaat cgcttctttt acttcttttg
    18661 ttacttctct tttctcattt aataaagtac catcgatgtc gatagcgact agtttgatag
    18721 acataatttc ctccaatata ttttaattta tttcaatcaa tgcgccatta cggatatggc
    18781 tggcaaattc ttgataagtt tcgtcaaata aatcataatg atcacgcaag gacggatcaa
    18841 ccatttcttt tggaaagaaa aatcgctcat ctccttgttc ttgtcccgct aatgcagcaa
    18901 caagctgact aacctgtgat aattctatca aagttccgtc atttcgcaaa atctcgattt
    18961 gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata aggtagatcg tagctagaat
    19021 tcacagcagt gtaataaaca ggattatatc cgaccttttc tactaacaaa gtgagttctt
    19081 ggactaattt tgaatcccgg ttgccagaaa aagtagcaga tttcagtggt ttacgattca
    19141 aaaaacgttt agctaaatca tttaaaatag gatcagcaga ttcagaccat tgagtaaagt
    19201 aagtgcccaa gaccccatca tcaagtttga ggtattcttc taaagtaaag cttccgtcaa
    19261 gaaatggcag aagtaattga gaatggagtt caaaactgct ggcatcttct tgatacaact
    19321 cgtgtgcgcg atgaagcaga tgctccaaaa ttacttccat accacgggaa acaggatgga
    19381 agtacacttg cacatacatt tgatatcggc taacgatata atcttccact gcatgcatgc
    19441 cactcatgga aaaagcgatc ccgccttcat aaggacgaat cacacgtaat attcgggtaa
    19501 gatcaaatgt tccatattct gttccagtga aataagcatc ccgaagcaaa tagtccattc
    19561 gatctgcatc gatctgacta gaaatcattt ggacaacttg tgggttgggg taagtttttt
    19621 ggatcacact agctaccttt tcaggaaaac cttcttctac tcggttaaga atctgataca
    19681 cttctgtttc tggagaagtg atgatctcta cagtgatggc ttcatgattc gtatggaaga
    19741 tatgttcaaa tgtatgagag taaggaccat gaccgacatc atgcaacagt gccgcacata
    19801 aagtcaccag ccgttcctga tcatcccagc ctccgttgcc tattttttct tttgaaaaat
    19861 ttcgagaaaa gatatcgcag attcgtcttg aaatctcata aacgcctaat gaatgagtaa
    19921 aacggctatg ttctgctccg tgaaaagtga aagaagaagt gcctaattgt ttaatgcgtc
    19981 gtagacgttg gacttctttc gaattgatca aatctaagat tacttgatgc tggacatgga
    20041 tatagttatg gactggatca cgaaaaactt tttccatagg cagcatttga tatttataag
    20101 gggttgtcat aatgtagctc ctttatgtta ttacgttgtt ccattctctg gatttggttg
    20161 ttccattgct ccagctggat ttcttttttt agccagtcgg tagaatcctg taaatcactt
    20221 gcagaataca gcttttcaaa agcttttgca aatgcttctt tgacttcttt tacaaaaagg
    20281 ggttggtcaa gcagttcttc aagtgttgcc attgtagcag gatccactgc aggatagcca
    20341 tttgtaccaa agtcttcttt taaagcagat tgataaaatc gcttcatgac ttgcccgcgt
    20401 ttttcctgat taccgttgac acttaagtac atcataacag aaatcccatt tttgatccga
    20461 cgctgagcga taccggcaaa ttttttgcca tggatgctta aatcataagt tccaggacaa
    20521 taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg gaaaagcgta tttagtcaat
    20581 aggagcattt gctcgtaagc gtcatctatg gttatttttt ttgtgtcact ttgggggaaa
    20641 atcaaagaaa tattcaaaat tccttcatct gatacgatcc ctaatccacc tgaatttctg
    20701 atcactggag cgtaacctgc atctttcagt gtttggatcc cgtcacttag aaaaggagtt
    20761 cgaacatcct tcatacctaa gatgactgtg cagtcatatt gccagaaatg taaaaagatt
    20821 tgctggtgtt ctttagaata ttccgttaat atatctgtta atgcaaaagg ggaaaagtcg
    20881 tttttttgat aaatgtgctg atctagtcga atcgcagggt tcattcaacc ggctcctttg
    20941 tataaattca tattttctac aatattatac tctaaaatca aagagttgtt ttggaaaaaa
    21001 ggttttctgt aaaaatggac aagttggttg acaaaacaaa agaaactagg aacaatgtag
    21061 ctatgagaag taaaggagaa tgaataatgg aattatcaca aggaacaccg atttcaatca
    21121 aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga tttttatttt gatttgaaag
    21181 ggcaaatggt gacaattggc gatacgctgt acatacgtta taaagagatt caggaggaga
    21241 cgggcgatga gattcctgta acgatcaaat tagttccaga tggtcatgta cagctgatcc
    21301 gtgcaggaga aatgcggatg cgtttgaaat ttggctataa agaacggtta gaaacatcct
    21361 atcgaacacc atacggtatg atccagattg ctacttttac gaaagaactg catgttagtt
    21421 tgaaagaccg tcccacagga gggaaggttc gaatcgatta tgatctgttc atgggaccgg
    21481 aaaaaatcgg cgaatattat ttgaccttgg attttacagc gtaaatataa aagaaaagtt
    21541 tgattttttg tgtctgattt tgtatgataa ggagtagact gtgaaaggac gtgtcctgat
    21601 tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt atcaatgatc gaagtagcac
    21661 acgcgatctt agaacaacgt ggcgatgtca tggatttttc tgatttagct aaccagatcc
    21721 aaaactatct tgggaaatca gacagtgaaa ttcgtgattc actcgcacaa ttttatactg
    21781 acttgaatat tgacggcagc tttatctcat taggggataa tcgttggggc ttgcgttcat
    21841 ggtatgctat cgattcgatc gatgaagaag taaaccatgg aatggacgaa gaggatgaag
    21901 atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt tatcaatgat gatgaagacg
    21961 cgatcgatta caatgacgat gacccagaag atacagatct gacagaagaa gatgacgatg
    22021 atctgtttga tgacgatgat gatgaagatg aagaaatcgc tgcttataat tctgacttgc
    22081 aagaaatcgg agcagatgac ccgacagatg atgaagaaga cttgccagga aatatcgaag
    22141 aagatttaag tatcatagac gaagacgatg atgatgaaga ttatgaagag gaagaatttt
    22201 ctgactctga aaaagaataa acattatttt cttacgagag gttgtgacag agacgatcag
    22261 cctatcagaa taaggaggtg tgacacgatt tttgtcacac cttctttttt gtatataaaa
    22321 atcccaagta aatacataaa aaataaaaaa attgaaactt ttagcgcacc ttgtacgtca
    22381 tattaagtga ggcagaaagg agtggagaat gatttttact ggatataaga aaaaacggca
    22441 gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa actgattatc aaaaaatgtt
    22501 ccgtatcacg atgaattacg tacatgataa agaagaggcc ttagatgtga tgcaagatag
    22561 ttttcataaa gccctgaact attttgaaaa agaaaaagag atcgagcatt ttagtgcatg
    22621 gttttatcgg atattgatcc gcacggcatt agatagttgg cgcagacaaa aaaggacgcc
    22681 tgtggactta tttgaaattc aagatatccc ccaacagtta cctttagaaa cttccgttgc
    22741 agagctccat atgattttag ataaaataga cagtccggaa aaagagattc tgatcctcta
    22801 cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc ttggatatga atgaaaacac
    22861 agttaaaacg aaaatgtacc gctcattgaa tgctttacgt caaatcctag aatgaaggag
    22921 agaagagtgc catgtattcc caaaaagaaa atttaaaaaa actaaagaaa acttataaag
    22981 aacaagcaat tcccaaaggg cttaaagagg agatccgcca gcgttttatt atcgaagagc
    23041 aatcatttat aaaagcaaag cggagaaaga gacgtctcca agggttcgga gtatctttgg
    23101 ctatcgtaat gattacaaca agtagccttc tttttaatag tcaagttcga agctttgcag
    23161 aagatttacc catcttagga tcagtcattg aattgatctt aggcgaacgc tttactgatc
    23221 gatctgaaaa aatcgatatt caggtaccga agatcaatac acaaaatgag aaagaaaata
    23281 aaacaattca tggattaaat caaaaatact ttcgcgaagg acaagcagat tttgaaaaag
    23341 ctgaaaaaga atatggaaat tttgaaacgg atcattacca agttttggga gattatcaga
    23401 aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca gatcacccaa acagcagcag
    23461 atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa aaacagtgtc cagctctcac
    23521 ttccactttt attcaaagat gacacctatc tatctgtttt gacaaaagaa gtcaaacggc
    23581 agatggccga acaagtcaag gaagatccat ctaagtacta ttggactgag caagatattc
    23641 aagaagggac gatagaaaaa ccaacacttg ttacgcctac acgaagtttt tatttgaaca
    23701 aagagcatca attagttttg actttttctc aatacgaaat tgctcctggt tatatgggta
    23761 caccagaatt tgtcattcct aagtccgtga caaaaacgat tttggcatcg gaagattatt
    23821 tggatcatta gacgagaagt agttgcgact agcagtttca attttcaagt aagtacaagt
    23881 aaataataag aagagaaaaa gagaaagaag agatatagtg aaaagaaatt atttagttat
    23941 cagtattatt gctttatccg tgcttttgtt tttcgaaatt acttcggtgg cctccttgat
    24001 caccgaagta aaaggacaaa cacactcttc ggagcattcg tcacaatctt tcaaacaatc
    24061 agaacaatca accattaaaa gccaatcaac tgaaacagct aacgtcaaag aagccattca
    24121 agaatcagcg gaaaaggtac aaagtcagtc atcacaatca acacaagaca gtacaaaaac
    24181 gagccgagat ccggctgtca aaagcgtcgc aatcagcttt gatgatggac caggagcgac
    24241 cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac gtgcatgcca cttttttcgt
    24301 cttaggagaa aatacggcgc agcatcctga aatcgtgaag cagacagctg aagcagggca
    24361 cgagattggc aattacacat acgatcatca gaatctggcc actctttctg cccagtcgat
    24421 gacggaggaa gtgacgaaag cagacactga gatcaaaaaa gtcactggca aaacccccac
    24481 ttttgttcgt ccgccctatg gttctgtcac gaatgtagga gcaacgatca tccagcgacc
    24541 gatcatcgaa tggtctgttg attcagaaga ttggaagaca agaaatcctg atttgatttt
    24601 gcagaagatc caagcaacag tatacgatgg ggcaattatt ttattccatg atatctatcc
    24661 tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg aaagagcaag ggtatcgcat
    24721 cacgacagtt ggggatttat tagggcatcc aacggctgtc gagaactatt atggcagaaa
    24781 cgatcatcgg ccagttcagt aagtgagaca agtatcttta ataaataaca cattttaata
    24841 agtagttgaa cacataacta ccttacttgc aaaggcttaa caatatctat acaggtacaa
    24901 caaaaaactg tccaccaatt ttctttggcg gacagttttc acttaaaaat aaagaagcaa
    24961 ctattcgtaa attatgctaa aatagcagta gaacgcgtcc tgagggaatc gaacccccgt
    25021 ctcaagaacc ggaatcttac gtgatatcca ctacactaag gacgcaagca ctgggataat
    25081 tgtaactaaa cagataaaaa attacaaggg gattggacaa aaaaatgaaa tttgagcaaa
    25141 atttttcaca aaaacagcag cagacacaga aactagccat gacgcaacag ttgcaacagt
    25201 ccatccaagt tttacaatat aacagtgagg agctgttggc attcgttgag aatcaagcaa
    25261 tggaaaatcc attggtggaa gttgttgagc cggaatggca gccagattac ataaaagctt
    25321 cttcatcttc ttatgaagga gaagagacaa attacctaaa tcagattcct gatacaaaag
    25381 ggtctttatt tgattcattg atcgaacaag tccatttgaa ttaccgagat acttttttga
    25441 ggaaaatcgt tctttatctt gtagaataca tcgatttgaa cggatttttg acgattagtt
    25501 tagaagaagc cggaaaagag attggagcta cgcctatcca aatgctcgat gcgttgactc
    25561 tgattcagca attagaacca gctggagtag gtgcacgtag tttgcaagaa tgtctcatgc
    25621 ttcaaacaga acgagatgat tatgcgccgg aattagctta tatcgtgttg gaagaatgtt
    25681 ttgaggaact ggtggagcga aaatggaaag aaatcgcaca acgtttttct gttgatctac
    25741 atgctgttca gcagatattc gactatttac aaacgttgag tccaagccct ggccggattt
    25801 tcgaccgtag cagcgaattg tacattattc ctgatgtccg ggtattagtg gacgacgata
    25861 aaaatgtaca agttatctca aaccgaaaaa atcagccaaa tatacgcttt caagagagtt
    25921 acttcaaaca aatgtcacaa caagcagata aagaaactga aaattattta aaagaacgga
    25981 aacaggaatt cgaatggttg aagaaaacga tcctacaacg tggggataca attttgcggg
    26041 ttgctcaagt cattgtttcc cggcaaaaag ctttctttat cgacaaggaa cgtccaatca
    26101 agcctcttac gttaaaggaa gttgcaactg agatagatgt gcatgaatcc acggtcagtc
    26161 gtgcagtcaa cgggaaatat ttggaaacag atttcggtgt atttgaatta aaaaaatttt
    26221 tcactacacg aattcctacg aatagtacag aacagacaga agacctttca gcagatacag
    26281 cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa aaacaagccg ttgtcggacc
    26341 agaaattggt tgaattgctg aaaaaagatg agatagcgat ctcaagacga acagtagcaa
    26401 aatatcgtga tttattagga atcccgagtt catcaaaaag aaaacgttat gataactaat
    26461 cagtcaaaag actagaaaaa gtatttcact ttttctagtc tttttagttg aaaaacgaga
    26521 gcgaaactgt tagacttaca gtcgtgaggt tgagatttcc ctttattgat gtgaaattcg
    26581 aatagttaat ttcttttttt atcgtatatg ggtcgtttta agtctacatt ggacgtaaag
    26641 tgtccaacta aggagatcgc tatgctggat gaaataaaaa tgattgaatc tgttgcacca
    26701 gatattatcg aagtgttgca agaacgctat aaaattttac gaaatatcta ttggatgcag
    26761 ccgattgggc gtcgaagttt atcggaaagt atggggatca cagaacgagt cttacgaaca
    26821 gaaacagacc ttttgaaaaa tctgaatttg atcgacacct caaaaagcgg tatgacgctg
    26881 acaactaagg gcgaagaagt ttatcaaagc cttgaaaact tcatggatca gcttttaggt
    26941 acgcatcaaa ccgaacaaca gttggcagaa tattttggta accaacgctg tatcgttgtt
    27001 tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat tcggcgaagc gctgagcgaa
    27061 gccttagacc gactacttcc agaaggcgag aatatcatcg ctgtgatggg aggaactact
    27121 atggctgtgg tagccgaaca attgtctaat ctggaaaata agaaacggca taacctgttt
    27181 gtccctgcaa gaggcgggat tggtgaagcg ataactgtac aggcaaattc tgtcagtgca
    27241 agaatggctg ctaaagcaaa cggtaaccac cgcgcacttt atgttccgga acaattaagt
    27301 ctggcaactt acaattcttt actcaatgag ccatctatcc aagaagtctt aaacttgatt
    27361 agtgaagcga attgtgttat tcatagtatt gggcgtgcat tacacatggc agcacgtcgt
    27421 aagatgactg aaaaagaatt agtcatgctg aaacaagcaa atgctgttgc ggaatctttt
    27481 ggctactttt tcaatgaaaa aggtgaagtc gtttataagg tccctagaat cggcatagaa
    27541 ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg cgggcggaaa atcaaaagct
    27601 aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa cgtggctcat cactgatgaa
    27661 gctgctgcaa atgagatttt aaaaggggta accctttaaa ataaatattt ttttgatttt
    27721 cataaggagg aaatcttaaa tgacagttaa agtaggtatt aatggttttg gacgtattgg
    27781 acgtctagca ttccgtcgta tccaagatgt agatggaatc gaagtagtag caatcaacga
    27841 cttgacagat gctaaaatgt tggcacactt gttaaaatac gacacaactc aaggacgttt
    27901 caacggaaca gttgaagttc atgaaggttc attcaacgta aacggaaaag aagttaaagt
    27961 attagctaac cgcaacccag aagaattacc atggggcgaa ctaggcgtag atatcgttct
    28021 agaatgtact ggtttcttca cttctaaatc agcagctgaa aaacatttaa cagctggtgc
    28081 taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt ccaacaatcg tttataacac
    28141 aaaccacgaa acattaactg gtaaagaaac agttatctca ggtgcttcat gtactactaa
    28201 ctgtttagct cctatggcta aaacattgaa cgacaaattt ggtgttgttg aaggattaat
    28261 gacaactatc cacgcttaca caggtgacca aatgactcta gacggacctc atcctaaagg
    28321 tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt cctaactcaa caggtgctgc
    28381 taaagctatc ggtttagtaa ttcccgaatt gaacggtaaa ttagacggag ctgctcaacg
    28441 tgttcctgta ccaacaggtt cattaacaga attagtaaca gttcttgaaa aagaagtaac
    28501 tgttgacgaa atcaatgcag caatgaaaga agcttcaaac gaatcttacg gatacaacac
    28561 agacgaaatc gtttcttcag atatcgttgg tatgacttac ggttcattat ttgatgctac
    28621 acaaactaaa gtaatgacag ttggcgacaa acaattagtt aaaactgttg cttggtacga
    28681 caacgaaatg tcatacactg cacaattagt acgtacttta gaatacttcg ctaacttata
    28741 agatagacaa acgtacaagt gtaaattata gacatgtaca tttaaaagcg gggaagcaat
    28801 cgcgcttctt ccgctttttt tgataataag tttattcagg aggaaataaa atggctaaga
    28861 aaacagtgaa agatatcgat ttaaaagaca aaaaagttct tgtccgtgtt gacttcaacg
    28921 ttcctttgaa agacggcgtg atcactgacg acactcgtat caaagcagca ttgccaacaa
    28981 tcaactacgt tctagaacaa ggcggaaaag caatcctttt ctctcacctt ggacgtgtaa
    29041 aaacagaaga agataaagaa ggaaaatctt tagctccagt tgcaaaacgt ctaggcgaat
    29101 tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg agaacaatta gaagaagcaa
    29161 tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa tacacgattt gaagatattg
    29221 acggtaaaaa agaaagcaaa aatgatacag aactaggtaa atactgggct tcattaggcg
    29281 atgtatttgt taacgatgct ttcggtacag ctcaccgtgc acatgcttct aacgtaggta
    29341 tcgcttcaac tggtatccca acagttgctg gattcttgat ggaaaaagaa atcaagttca
    29401 tcggtgaagc agtagaagaa ccaaaacgac caatgattgc aatccttggt ggcgcaaaag
    29461 tttctgataa aatcggcgta atcgaaaact tgattcctaa agcagataaa atcttgatcg
    29521 gtggcggaat gacttacaca ttctacgaag caaaaggaat caagatcggt aactctctag
    29581 tagaagctga taaagtggaa ctagcaaaag aattaatcga aaaagcaggc gacaaacttg
    29641 tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa tgatgttgaa acacaagtaa
    29701 tcgaaggaga cattcctgat ggtttgatgg ctttagacat cggcccagca tcagtaaaat
    29761 tatttgctga tacattacaa ggtgcaaaaa cagttgtttg gaacggacct atgggtgtat
    29821 ttgaaatgag taactttgct aaaggtacaa tcggtgtttg tgaagcaatc gctaacttag
    29881 aaggcgcaac aacaatcatc ggtggcggag actcagccgc agcagcagaa caattaggat
    29941 ttgctgacaa attcacacac atctcaacag gtggcggcgc aagcttggaa ttgcttgaag
    30001 gtaaagaatt accaggacta gcagcaatca acgataaata aacgaccaag tttgctcaat
    30061 tctgacgagt aatcgaacaa aaggtgaagg gagttttcat gactccctag ccatttttca
    30121 ataaattaag gagtgtgttc ctcaatgcgt aaacctatta tcgcaggtaa ctggaaaatg
    30181 aacaaaactg catcagaagc aaaagaattt gctgaagcag taaaaaacaa aatcccttca
    30241 aatgacaaag ttgattcagt catcggatct cctgcattat tcttacaaga attaatgaca
    30301 tcagctgaag gtacagaact aaaaatctca gcacaaaact gctactggga aaactcaggt
    30361 gcgttcactg gtgaaacttc accagcagct cttgctgatc taggtgttca ttatgtgatc
    30421 atcggacatt cagaacgtcg tgaatatttc catgaaacag acgaagacat caataaaaaa
    30481 gcgaaagcta tctttgcaaa tggtatgaca ccaatcctat gctgcggcga atctttagaa
    30541 acctatgaag caggcaaaac tgctgaatgg atcgaaggac aaattacagc tggtttagct
    30601 gatctttcag ctgaacaggt gtcaaacatg gttattgctt atgaaccaat ctgggcaatc
    30661 ggtactggta aatcagctga cgcacaaatc gctgacgaaa tttgtggagt tgtacgtcaa
    30721 acagttgaaa aactttatgg aaaagaagta tcagaagcag ttcgtatcca atacggcggt
    30781 tctgtaaaac cagaaaatat tgctgaatac atggcaaaag aaaatgttga cggtgcttta
    30841 gtcggtggcg caagcttgca agcagattca ttcttgtcat tgttagacgc tgtaaaataa
    30901 ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact aatgagcaaa attaactaaa
    30961 atcgtatatg agggaaatcc cttaggaaaa aacaaactca aaggagagac aaaacatgtc
    31021 aattattact gatgtttacg ctcgcgagat cttagactca cgtggtaacc caacaatcga
    31081 agtagaagta tacacagaaa gcggagcttt tggccgcgga atggttcctt caggtgcttc
    31141 aactggtgaa tacgaagcag ttgaattacg tgacggagac aaagctcgtt atggcggtaa
    31201 aggtgtaact aaagctgtcg acaacgtaaa caacatcatt gctgaagcaa tcattggcta
    31261 cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc gctttagacg gaactcctaa
    31321 caaaggtaaa ttaggtgcga acgctatttt aggtgtttct atcgctgttg cacgtgcagc
    31381 tgctgactac ctagaagtac ctttatacca ctacctaggc ggattcaaca caaaagtatt
    31441 gccaacacca atgatgaaca tcatcaacgg cggatcacat gctgataact caatcgactt
    31501 ccaagaattc atgatcatgc ctgttggcgc tccaacattc aaagaagctc tacgtatggg
    31561 tgctgaagta ttccacgcat tagcttcaat cttgaaagct cgcggattag caacttctgt
    31621 tggtgacgaa ggtggatttg ctccaaacct tggttcaaac gaagaaggtt ttgaagtaat
    31681 catcgaagca atcgaaaaag ctggctatgt acctggtaaa gacgttgttc ttgctatgga
    31741 tgctgcttct tcagaattct acgacaaaga aaaaggtgtt tacgtactag ctgactcagg
    31801 cgaaggcgaa aaaacaactg acgaaatgat caaattctac gaagaattag tttctaaata
    31861 tccaatcatc tcaatcgaag atggattaga cgaaaacgac tgggacggat tcaagaaatt
    31921 aactgacgta ttaggcgaca aagttcaatt agttggtgac gacttgttcg taacaaacac
    31981 tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac tcaatcctta tcaaagtgaa
    32041 ccaaatcggt acattaacag aaactttcga agctatcgaa atggctaaag aagctggcta
    32101 cacagcagtt gtatctcacc gttctggtga aacagaagat tcaacaatct ctgatatcgc
    32161 tgttgcaaca aacgctggcc aaatcaaaac tggttctcta tcacgtactg accgtattgc
    32221 taaatacaac caattactac gtatcgaaga ccaacttggt gaagttgcag aatacaaagg
    32281 tttgaaatct ttctacaact taaaaaacaa ataattcttt tctaatgaag atacatgatc
    32341 gactattgaa agatagtcga gtatgatcac ttagttaagt aatcgaactc tctacttgct
    32401 gaaatatcag caggtagaga gtttttttgc aactagcttc tgtatatgat gtttacaaag
    32461 aatggtacaa gaaaagatca tccttgaaaa aggaaagcta acaataacta gcatcatata
    32521 agagctttta aggatagaac agtagaatag gaatctatgg gcaagtcctc tcgtataaat
    32581 aaaataggag ggaagtagca gcatattttt cataaagtgg ctctctttct tcatttttac
    32641 tttggttgct cctatttgaa aattcgagca cactcaaata caataaaaag aaatggcaag
    32701 ggagaggatt agatgaaaaa agaagaaaaa atcgcgattt tacaagagat tatccgaatt
    32761 aaatcagtga atggaaacga aggtgaagta gcagcctatt tgaataagct tttggcaaag
    32821 catgacataa caggagagat tgtttcttac agagatggta gagataattt gattgctcgt
    32881 taccaaaagg gccaatcagg aaaagtgtta ggcctatctg gacatatgga tgtcgtggcg
    32941 gctggtgatg aatcgtcttg gacctatgca ccctttgcag cagaaatcca tgggaaccgt
    33001 ttgtacggac gtggtgctac agatatgaag agtggcttgg cagcaatggt gattgccatg
    33061 atcgaactga aagagtcagg aaaaccattt aacggaacag tcaaattatt agccactgtc
    33121 ggagaagaag tgggtgaact aggtggggaa cagctcacga aagcaggcta tgtagatgac
    33181 ttagatgcct tgatcatcgg agagccaacc aattacagtt tgatgtatac gcacatggga
    33241 tcaatcaact atacagtcac ctcgcacgga aaagaagcgc atagctctat gccagaccaa
    33301 ggttataatg caatcaatca cctaaatgaa ttcattacga aagcaaatgc tgaaatgaat
    33361 cacctcgctg aaacaatcga gaatcctgtc ctaggaaaaa caatccataa tgttacattg
    33421 atcagtggcg ggaatcaagt caacagcatc ccaagccatg cgcagctcca agggaacatt
    33481 cgatcgattc cagaataccc aaatgataaa atcattgcat tgctgcaatc aatcgtcaat
    33541 gaattgaatc aagagacaga ctatcatttg gaattgatga tcgattacaa caaaatacct
    33601 gtcaaagctg atccagattc tcctttgatt cattgcattc agcaacaatt cagccagcca
    33661 ttacctttag ttggagcggc agcaacaacg gatgcagccg aatttacgaa agctaaccat
    33721 tccttcgact ttgtcgtctt tgggcctggt gtcgttactt tgcctcatca agtagatgag
    33781 tatgttgaaa tcgataatta tttggatatg atcgaaaaat atcaaggaat tattttatcc
    33841 tacttagcgt aagatttttt taat
//
LOCUS       DAPCMT010000015        33130 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPCMT010000015 DAPCMT010000000
VERSION     DAPCMT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.015"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..578)
                     /locus_tag="RD743_000588"
     CDS             complement(<1..578)
                     /locus_tag="RD743_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDU0562445.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(655..1185)
                     /locus_tag="RD743_000589"
     CDS             complement(655..1185)
                     /locus_tag="RD743_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDU0562446.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1189..2049)
                     /gene="rlmB"
                     /locus_tag="RD743_000590"
     CDS             complement(1189..2049)
                     /gene="rlmB"
                     /locus_tag="RD743_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDU0562447.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2042..2437)
                     /locus_tag="RD743_000591"
     CDS             complement(2042..2437)
                     /locus_tag="RD743_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDU0562448.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2434..3846)
                     /locus_tag="RD743_000592"
     CDS             complement(2434..3846)
                     /locus_tag="RD743_000592"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDU0562449.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3843..4379)
                     /gene="cysE"
                     /locus_tag="RD743_000593"
     CDS             complement(3843..4379)
                     /gene="cysE"
                     /locus_tag="RD743_000593"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDU0562450.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4465..4704)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4701..6161)
                     /locus_tag="RD743_000594"
     CDS             complement(4701..6161)
                     /locus_tag="RD743_000594"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDU0562451.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6331..7488)
                     /locus_tag="RD743_000595"
     CDS             complement(6331..7488)
                     /locus_tag="RD743_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDU0562452.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7522..8892)
                     /gene="radA"
                     /locus_tag="RD743_000596"
     CDS             complement(7522..8892)
                     /gene="radA"
                     /locus_tag="RD743_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDU0562453.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8955..9482)
                     /locus_tag="RD743_000597"
     CDS             complement(8955..9482)
                     /locus_tag="RD743_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDU0562454.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9712..10518
                     /gene="proB"
                     /locus_tag="RD743_000598"
     CDS             9712..10518
                     /gene="proB"
                     /locus_tag="RD743_000598"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDU0562455.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10515..11762
                     /locus_tag="RD743_000599"
     CDS             10515..11762
                     /locus_tag="RD743_000599"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDU0562456.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11838..12182
                     /locus_tag="RD743_000600"
     CDS             11838..12182
                     /locus_tag="RD743_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562457.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12299..12625)
                     /locus_tag="RD743_000601"
     CDS             complement(12299..12625)
                     /locus_tag="RD743_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562458.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13240..14442)
                     /locus_tag="RD743_000602"
     CDS             complement(13240..14442)
                     /locus_tag="RD743_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0562459.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14559..15074
                     /locus_tag="RD743_000603"
     CDS             14559..15074
                     /locus_tag="RD743_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDU0562460.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15273..15719)
                     /locus_tag="RD743_000604"
     CDS             complement(15273..15719)
                     /locus_tag="RD743_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDU0562461.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15766..17244)
                     /gene="msr(C)"
                     /locus_tag="RD743_000605"
     CDS             complement(15766..17244)
                     /gene="msr(C)"
                     /locus_tag="RD743_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDU0562462.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17598..18227)
                     /locus_tag="RD743_000606"
     CDS             complement(17598..18227)
                     /locus_tag="RD743_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDU0562463.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18211..18675)
                     /locus_tag="RD743_000607"
     CDS             complement(18211..18675)
                     /locus_tag="RD743_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0562464.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19162..20430)
                     /locus_tag="RD743_000608"
     CDS             complement(19162..20430)
                     /locus_tag="RD743_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDU0562465.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20661..21398)
                     /locus_tag="RD743_000609"
     CDS             complement(20661..21398)
                     /locus_tag="RD743_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0562466.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21465..22034)
                     /locus_tag="RD743_000610"
     CDS             complement(21465..22034)
                     /locus_tag="RD743_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDU0562467.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22162..23034
                     /locus_tag="RD743_000611"
     CDS             22162..23034
                     /locus_tag="RD743_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0562468.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23376..24248
                     /locus_tag="RD743_000612"
     CDS             23376..24248
                     /locus_tag="RD743_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0562469.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24245..26203
                     /locus_tag="RD743_000613"
     CDS             24245..26203
                     /locus_tag="RD743_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0562470.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26535..27386)
                     /locus_tag="RD743_000614"
     CDS             complement(26535..27386)
                     /locus_tag="RD743_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0562471.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27509..27976
                     /locus_tag="RD743_000615"
     CDS             27509..27976
                     /locus_tag="RD743_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDU0562472.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28239..28721)
                     /locus_tag="RD743_000616"
     CDS             complement(28239..28721)
                     /locus_tag="RD743_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDU0562473.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28821..29684)
                     /locus_tag="RD743_000617"
     CDS             complement(28821..29684)
                     /locus_tag="RD743_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDU0562474.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30282..31157
                     /locus_tag="RD743_000618"
     CDS             30282..31157
                     /locus_tag="RD743_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562475.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(31302..31454)
                     /locus_tag="RD743_000619"
                     /pseudo
     CDS             complement(31302..31454)
                     /locus_tag="RD743_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(31722..33053)
                     /locus_tag="RD743_000620"
     CDS             complement(31722..33053)
                     /locus_tag="RD743_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0562476.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         9655 a         6923 c         5919 g        10633 t
ORIGIN      
        1 tattcctcat tataaaattt caagtgtttt tttattttga gttttactct cccgtaagcg
       61 ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa atcaacgcct
      121 tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc gcaattttcg
      181 attgcttctt ttaataaaag atgctgaatt gctaaaggtt cttgcaatgt gatctctgct
      241 aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc tttttgatca
      301 cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct tataacgctt
      361 ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa tgcctgatat
      421 aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa ataataggtg
      481 tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa tgcttcttcg
      541 tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct ccataaattc
      601 gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga tatgttattt
      661 attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct cttcattcca
      721 tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg ctattgtttt
      781 ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg ctccttggga
      841 aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg tcaaacggtc
      901 attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt ctttcgtaaa
      961 aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta gttgggcatc
     1021 gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac gatgtaaaag
     1081 agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt ccggccacga
     1141 accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct actccccctg
     1201 acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat ttaagctctg
     1261 gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca atcctggact
     1321 cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat tccatttttg
     1381 gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt tgagctgttg
     1441 cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg ccccagtgga
     1501 agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga tcacaccatc
     1561 gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat cttcgatgct
     1621 atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa tcagttggtc
     1681 aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca cgccgtgatc
     1741 gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt gttcacgtgc
     1801 gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa agagtttatt
     1861 ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga aattatcttc
     1921 taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct ttttatttga
     1981 atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt ttgttctttc
     2041 tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct tccatccgct
     2101 cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt gcgacccgat
     2161 acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt cctctgcgat
     2221 aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga atcaaaaagg
     2281 cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg ggttttgttt
     2341 ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct ccaacataag
     2401 ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc atcgggttcc
     2461 ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa tttcgtcgct
     2521 tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt gttcaatctc
     2581 ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc attgcgagaa
     2641 taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta gataagtgtt
     2701 cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa aatcatcgtc
     2761 catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat ccgcatcatc
     2821 tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg tattcttcag
     2881 tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga tcggacgacg
     2941 gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt tgatcatttc
     3001 atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt cgccaatcgt
     3061 tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg cttcactttg
     3121 tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat ggatatcgat
     3181 cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac cgggacgtcc
     3241 tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc ataaagcgaa
     3301 atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg ctccgacttc
     3361 cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac gggtacggta
     3421 atacacatca cctgaagatt cataagcata gcctttttca accaacactt gaataaatgc
     3481 taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggtt cgacattcaa
     3541 cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg gtgctgtaat
     3601 acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat tcgatacgaa
     3661 atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag caatggtgct
     3721 acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat acataccgac
     3781 atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat tgtagatttt
     3841 tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc tggtactcct
     3901 accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc gattttggcg
     3961 ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat ggctccttgt
     4021 ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt tacgccatgg
     4081 aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc cattccatgg
     4141 tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc agttaggaaa
     4201 cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata taaaaaatga
     4261 gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat ctcagctgtc
     4321 gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa ccaactcatg
     4381 actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata aagaaaagaa
     4441 gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc acttttcttc
     4501 ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct actttgtttc
     4561 ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag aggcatttcg
     4621 gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat cagctgctta
     4681 cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa tgatttttca
     4741 cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc tgatacagct
     4801 acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg aacagctttg
     4861 atggcagctt tgacagttgg agcatctact acttccattt gttctagctg tgctttgaat
     4921 gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc ttcattcaat
     4981 acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc atagctcatt
     5041 tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc atctgggttt
     5101 tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa tgcatctaga
     5161 tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc gaaagctgcg
     5221 ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg ttcgaagatt
     5281 tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat tgcatctggt
     5341 agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc acgtttgctt
     5401 agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt tggtggtgtc
     5461 cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg gtcatctcca
     5521 cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa attataagtt
     5581 ggcataccat cgcgtttttg gatgacaaag tcgccaccga tattatcgga ttcgaacgaa
     5641 atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac acggaaacgt
     5701 acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt caagttcgca
     5761 catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg ttctgcttct
     5821 aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag ctgatcaatc
     5881 agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc tccaggattt
     5941 tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg gcttttttcg
     6001 ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa gtctccatca
     6061 ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat atgcaggtgc
     6121 ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct cttctttcaa
     6181 actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat atcatttatt
     6241 taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc aactggttga
     6301 atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct ttttctctgt
     6361 ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga tcatgcgtcc
     6421 agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca tgtaatgctg
     6481 tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt gttggcgttc
     6541 tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga ctggttttac
     6601 ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag agactttatt
     6661 caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt tgatcaattt
     6721 gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag atataccctc
     6781 ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac gtttcaaact
     6841 atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga tcatcaaggt
     6901 tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga tcacgcttgt
     6961 atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca gttgactctc
     7021 aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa ctttcttcca
     7081 ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga tcatcagtaa
     7141 aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg tcgagatcac
     7201 aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat aagtcaagct
     7261 gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt tggccaacag
     7321 caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg tattattcag
     7381 ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg aaatacccaa
     7441 gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg tttttcctcc
     7501 gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag ccaaagttgc
     7561 tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt ttttaggtag
     7621 gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa tatgactgac
     7681 acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg gctgtgttcc
     7741 tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg ctggttcatt
     7801 taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa gcaacccagc
     7861 tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac ctgttgttgt
     7921 tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta ccaaaattgg
     7981 acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta ggcgttcttc
     8041 taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca tttcaaaaat
     8101 cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc gaaaactatg
     8161 atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca ttcttgggcc
     8221 ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc catttgtttt
     8281 agcggttttg agtagttctg cagtggtttc tcttacttgg ctgacacttc ctgccacact
     8341 tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat caggggagat
     8401 atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat aaaattctgt
     8461 gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt cttcgccgga
     8521 tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta acagcaaagt
     8581 ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca ccacacctcc
     8641 gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt ttttagggac
     8701 gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc gagttctgcg
     8761 gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc aatttgggca
     8821 gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt gtacttttga
     8881 tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa tcaagcttaa
     8941 gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg agcagtcgtt
     9001 tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg ctcgcctttt
     9061 ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg gccttcattt
     9121 gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa cactaaaaac
     9181 cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc tcccatgtaa
     9241 gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc tgatacatct
     9301 tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt ccagatactt
     9361 ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg tgtagcgcgc
     9421 tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc tctttttctc
     9481 accggttttc ctccctaaat aaactactcc tattctaaca tatccgaggc tgtaaaagac
     9541 tttctttaca aattcttcaa gaaattctgc tatcttaaag taaaccatta attcgttctt
     9601 tttccaaacg actgcaagtt gtacaaaaaa actatttttt acatgacttt actcgtcaaa
     9661 acaagtagaa tagaacgaaa ttaatgagtg gatatttgat tgaggtgtca catgcgtaaa
     9721 gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa caagtacatt gatttatccc
     9781 aatggaaata tcaatcttgc ggcaatcgat cagttagctt ttgtcctttc tgatcttaga
     9841 aatcggggaa aggaagtgat cttagtctca tcaggagctg taggcgttgg tatgcataaa
     9901 ttacatttgt cagaacgtcc gactttgatt cctgaacagc aagctgcggc ggctgttggt
     9961 caagccgaac tgatgaatat ctataatcaa agattcagtg tatataatca acagacggca
    10021 cagatcctcg tgacaagaga cgtggtagat tatcctgaaa gtcgtaaaaa tgtgaggaat
    10081 acattggaac agcttttgat tatggacacg atccctatcg tgaacgaaaa cgatactgta
    10141 gcagtggatg aattagatca tctgacaaaa tttggcgata atgatcagct gtcagccatc
    10201 atggcaaaac ttgtcgcggc agaccttttg atcgtccttt ctgatatcga cgggttttat
    10261 tcagataatc cgactacaaa tcccgaggct cagatgtatc gctatatcga taaaatcgat
    10321 gaaaagattc tggcacaagc aggtggtgct ggtagcacat ttggaacagg cggtatgcag
    10381 agtaaattaa aagccgcttc taggatattt gaagcaaaca gcgcaatgat tttagcaaat
    10441 ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa aagaagtagg tactttattt
    10501 aaggaggact tgaaatgact gacttgatgc aattaggaca acaggcaaaa gatagcgcgc
    10561 aacaactagc acttatgcct acaaaccgca aaaatgagct acttgttcaa atggcacaaa
    10621 ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca taaagactta gaaaaagcag
    10681 tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca gctgacacct gagcgaatca
    10741 atgccatggc tacagagatc gaaaaaattg cttcgttaga cgatcctatt ggaaaagtag
    10801 atgagatgtg gacgaacacc gacggattga gaatcggcaa aaagcgcgtg ccactaggcg
    10861 tgatcggcat catttacgaa tctcgtccaa atgtcacgac agatgcagct agtctagcat
    10921 tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga agcattcttc tcgaatcaat
    10981 tattagtcca attattgcag caagtactgg tcagtgctgg cgaatctcct tatgccatcc
    11041 agtttgtcga tgatacttct catgaaactg caggacagtt gatgcgattg acagaatact
    11101 tagatgtgct gattcctcga ggcggagcaa aattaatcca acgagtcaaa gaacaggcaa
    11161 cggtacctat cattgaaaca ggtacaggaa ataatcacgt atatatcgat aaagaagcac
    11221 agttatctat ggcagtgaac atcatcgtca atgcaaaagc ttcccgtcca tctgtctgta
    11281 acgcggcaga aacattgctg atccattctg agatcgcacc ttttttcttg ccagctattg
    11341 aaaaagaact ggtagaacac ggggtctctt tacgtgcgga tgctcgtgcg ttagaatatc
    11401 tagagacggc tgctttagca gaagacacag attgggatac tgaatatcta gattatatcc
    11461 ttgctgtcaa agtggtcgat tctttggatg aagcaattgc acatatcaat cggcataata
    11521 ctaaacattc agaaacgatc gtgacagata gttatgcggc tagtcaacgt ttcttaaatg
    11581 aagtggacgc ggcggctgtc tatgtcaatg cctccactcg ttttacagac ggatcggtat
    11641 ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt gcatgcccgt ggtccaatgg
    11701 ggttaaatga attaacttcc acaaaatata tcatttatgg cgatggacaa atccgcgaat
    11761 aatgtttcgt gtgaaacatt attcccgcct tttgcctttt ttctgtaaag gttctatcca
    11821 actagaaagg tgtcaatatg tttgaaattt taatgatttt ttttatttac cttatttctc
    11881 tgaatatcgc tgcttttctg ggagtcggta ttttatccct tttctttcaa ttcaaaaaac
    11941 gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag
    12001 ggctggttac acgcttgcac attagttaca tggttgcatt atgcttatta gcgacaatca
    12061 attactattc ttttttcgat cattcaatcg cttatacgat tacgctactg attgctggga
    12121 tttttcactt atcttataaa tatcagctga ataaaaacca cttgaatcgg actttccgct
    12181 aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt
    12241 ttagaggctg tgacaaagat tttgtcacag cctctttttc catataaacg gcgttcgcct
    12301 accagctgcc tggtaaatct gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt
    12361 cgccatcata ataacgaagc agctcatcca tggacaagtt catcgttttt tctttttctt
    12421 cttccgctga ttcagccagt agctgttccc gatacacctg atacatacca gctatttttt
    12481 gctgcatcgc ttttctagtt tcaccttccg cattgattat tggcaattct ttgatatagc
    12541 cacgataaat caaccgatca tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga
    12601 cttcctgaaa atattcttcc agcatctatt atccccccat tctttttctt aaatgaattt
    12661 tagcttacct actgaaaatt ttccagtttc attattttga gaaaaaaagg gaatgtgaca
    12721 aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc
    12781 ataagtcaca atattcaaca aacatgagaa actgtttttt gaaattgttc cttgtgattc
    12841 agagttaaac gacagtctca caacctcttg gtaaacggcg ttcaatcagc tgaccctcgg
    12901 tattagctca aaaaacggta aaatatgaga agctattttt ccgttttgtt tctctaatac
    12961 ttaggataaa acgctgattt tacaacctct gttaaacggt gttcaatcag ctgtccttcg
    13021 gtattagctc aaaaaacggc aaaatatgag gaactatttt tccgtttgtt tctctaatac
    13081 tcaggacaaa acgctgattt tacaacctcc tgataaacgg tgttcaaaag cttgcatctg
    13141 cggtaataag cccaaccaaa cgaaaaatat gagaagctat ttttgtttgg ctgttcttat
    13201 gacttgatgc aggacagctt tttcacaacc tctgatttct tattcttctt catttgtagc
    13261 aaaataatta cggtgataaa tgatacaaca tacactgata agaacaatgg cacctgcaaa
    13321 ataaaacgca tagctttcac caatatgttc accaatcaca cctgcaataa atggtgctaa
    13381 agctccaccg aaaaagcgga caaaactata gctggacgaa gcaacgtttc gttgcagacc
    13441 tggtatttcc atagcaatcg tcgtcaacag tgtattgata attccttgga aaaatcctgc
    13501 taacacaatg ctgatcggta caaggactgg catagaagtt ccagctccta ataacagcaa
    13561 acaaataatg aataagacaa atgctgctaa aattgtccga taagtcgtaa acttccgctc
    13621 aagagccggt gcaacgaaaa tcgaagcaat agccagaagc acaccccagc caaaaaatac
    13681 aaaaccgact tctaattcag aatagccaat caataaaaat ggcgcatacg caagtaaagt
    13741 aaagaatccg aaattatata acaatgcgat tagtccaact gaacgaagtt tatgattgct
    13801 taacgctttg acaccttccc aaaggcctac ttttctcttt ggtttctcag tttcaggtaa
    13861 aagtaccgct agggctataa aaccgcaaaa catcaaaaat gctaccccaa aaaatggata
    13921 acgccatgaa ccactgccca agaccccacc gactagaggg ccaacagcca ttccgcagcc
    13981 aagtgctgct tcatacatca tgattgcttt ttctgtgtta ccaactaaaa tactgacgat
    14041 cgttgctaaa gcagtagaga caaataaagc attcccgaca ccccagccgg cacgtaaccc
    14101 aatcagcatt ccgatattcc cagataatcc gccaagacca gcgaatacaa cgatgataaa
    14161 caatcctgtc atcaatgttt ttttcaaacc gatccgacta gataagaaac ctgtaaatag
    14221 catcaccaca ccagtgacta acatgtaact agtaaataac agggtcgttt cagctggcgt
    14281 agcgtttaac tgtgcagcaa tcgtttttaa gattgggtct actagaccca ctcccataaa
    14341 agcgatcata caggtagctg ctacaactag cgctgaactt ttcccctttg tttcttcatt
    14401 tgtttgttct tgtatatcta tttgattctc caatatttcc atccctttct ctctattagc
    14461 atcatacatg atttatttta catgtcaata tgcacatgta aaacgtggaa actttactgt
    14521 gatataataa agaaaataag acgagaggta ggatttttat gaatacttta gcctatatat
    14581 tattatgcgt gcttttatcc aaaccacgtt ctgggtatga attgaagcag ctgatcacaa
    14641 ttttttggga agcccatcac agtcagatct atacaacgct tgccaagtta gaccagcagg
    14701 gttatgtgac cgttttggac aaagacgagc actctcaaaa aaagatctac cacttgactg
    14761 atgaaggacg tgctcttgta gaggaatgga tcaaagagga aactcccgct cctacgcaaa
    14821 aagatgaatt tctagcaaaa atctatgctt tttcgacgct cgataaaaca acagctactg
    14881 gactgctatt cactagagaa caacagctga acaaaatcat gcaaaaaaat caaaaaaaat
    14941 tgaatgacct ctcgctagac aagaaacaag aatttggacg ttatgttgtg atccagcgca
    15001 gaattatgct ttgtcagcag gaactccaat ggtgtcagca agtaagagaa caaatgcagc
    15061 agttttttga gtaagggata aacagaacct ttctagaaat atctattgaa gagcaaatta
    15121 tcttcaataa aagagcaata cagttggaaa agaaaaagcg atccgaacga caggccaaaa
    15181 cctatcgttc ggatcactat ttttctagaa ctgcattcaa gagactatga caaaaagcat
    15241 gccaaggcct tttttcaaat aacagtgttc catcattcat tactttgaat tgcttttagc
    15301 acacttcttg aaagcgggcc aacgatgatc aactgcaacg gtaaagcggc aatcacattc
    15361 attttccaag cattcatata agcagagaat aattgatcag gtattccttg ttccatgatc
    15421 acaccgaata aagacataca tgtcaccatt cctaaaacca tcaagcatga aatagttaag
    15481 atcatttgga ccttgctttc tggatttact ggtagtttaa aggcaatttt tttcgccagc
    15541 acaccgatga tgaagacatc caaaatgaaa gccaccacga atccaggcac tagaccaatc
    15601 aacaaacttg ataaatgaag atcttgatgc agccataaat tatacgcgct catccccaag
    15661 accatcaaaa aacacatcat tgttgtaaaa attattcctt cttttttatt tgtaggcaat
    15721 ctgtaccact cctttaaatt tgtcacgaaa atcatcgtag cacatttaaa aatttctcgt
    15781 aagtactttt tttattttct gcccttttat ttcataaact tcatcagccg tacgctcgac
    15841 aaagtaagaa tcatgagaag taaacaaaat cgttcccgga tatatttgca ttagcttctc
    15901 taaagcttcg atcgttgcca tatcaataaa attagtcggt tcatctaaca gcaacacatt
    15961 acttggctta gtaaaaagca aagcaatcgt cagacgggtc gcttctcccc cacttaatgt
    16021 acataacggt ttcgtcaaag tttcctgagc aaaccctaag tgattcagca aactacggac
    16081 aagcgattct gaaaactctg tttgtctcat gacaaaacga atgatggatt cttcagatgt
    16141 catttgataa cccttttgct gatatacttg aaagcttact ttcggagaga gaaggattcc
    16201 ttctccttgt ttgcggatat gttctaataa ggttgtctta cctgaaccat tttcaccgac
    16261 aatcgcgatt tttttaccta aagaaaactg aaaatcacca tttaccagta atgttctccc
    16321 acttctttcc aattggacat tttgtcccat gatgggatag cggctgtgga gttcaagaga
    16381 ttttggtact ggaaaacgga tttgtttcat ctgctgcggc ttggttgttt ctcctatttt
    16441 ttggagtctt ctctctaatg ttttcgcatt cttttggatg gctttttgta cactgtcttt
    16501 ttgtttagag gaagacaacc gatccagtct gatttgttgc tttttctttt tactggacac
    16561 tttttccaac ttttccgctt gttttttctt ttcttggatc gatttttcca atcgtttttt
    16621 ctctttcatg atcgactctg cttctcgtag ctgagtcttt ttctccaatt ccttttgagt
    16681 gagataggca ctataattcc ccgaaaactc tcggatttct ccgtctttta cttcccagat
    16741 tttcgatgct agctcatcta gaaaatagcg atcatgacta acaaaaacca gcgtaccata
    16801 ataataacga agctcttcaa tcagtcgatc aatacttttt ctatctagat gggttgttgg
    16861 ctcgtctaga agcatccctt gttcataaga agaaagaaga cgtatcaact cgatctttcg
    16921 ttcttcccct ccgctccatt tccgctctga agtttcttcc agtcttaact ggaaatgact
    16981 ggctaattcc aaatttaggt cctttgcttc agcgagatag gttaattgag gcaggtagtt
    17041 gaaggtgatc tctgtttgga ttttcccatg atcgggaaaa agttcaccgg ctattagttt
    17101 gagcaaagtc gatttaccga caccattttc tcctatgatc cctatacgat cttgctgata
    17161 gacacgaaga gaatcaatag atagttctag ctggttgcca aagctgactt gtagatttgt
    17221 tatatttact gctaaatttt ccatatgttt tacactccta tttcatgtaa aacaacaatt
    17281 atcgatacta aaaaagagca gaaaaatctg tttgcaaaaa aactgcatca catcttttcc
    17341 tactctgaac gaaataacta tgactaaatc accaaatctg tcatagcttt cacctattct
    17401 actgtcgttc gaaaaaatgg acatactcca tccatgaaat tgttgtttat acatgtcttt
    17461 tgaatttatt tagttgttat tcaaaagttc gaaacgtaat ttcatcgatg cagtcatttt
    17521 tccgacctcc ttttttatta ggttaccaca cacgattcaa aaaaagcaat agctactgaa
    17581 tagtaactga aaacctttta ttcgaacata gtgatctttt ctggtgctaa acgaacagac
    17641 tcgtaacctt cttctaccgc ttttcctatt gataaaataa taactggaac ataacggtct
    17701 ttatccaaac caaatgcctc tgccaactga tctgcttcga atccaccaat tggatttgtt
    17761 tcatagccat gatcacgcgc aactaacatg aattgcatag ccgctaagct agaatcgact
    17821 ttgactacat cgttcatttc ttctcttgaa aaactcttgt aataaggaat gatcgcagct
    17881 aattgttgat ctctgacttc ttttggcatc ttccctgatt cgtaagcttg gttgtagatt
    17941 tcttcgccat attcgtaaca ttgcatatcg ccaaaaatca tgaccattgc agaagaagta
    18001 tcattttgtc tcgtattgaa acggatcaaa ggacgcaaga tttcttttgc ttcctcgctt
    18061 tcagcgacca caaagcgcca tggtttcata ttgacagaag aaggtgcagt cgtcgcttct
    18121 ttgatcattt ccagcatttc ttcgtgtggg attttgtatg tttcatcata tacgcgaacg
    18181 gatcttctac cagtcataat tgttgaaaaa tcattttttt gttccattat tattaacctc
    18241 tttcgtttct gttgttatat tgcaaagttg ggtattcaat ttatctaaaa gtgttagaaa
    18301 ttgtttcatt tctacatcgc tgaaagccgg ggatagcacg gaacctgtat ctttatggtc
    18361 catttcacaa tgcttgattt tcttctgggc tttttctgtt agcgctacat acacttcccg
    18421 gttgttctga ggatttctct gacgcgaaac atagccattt gcttccaata ccttcagatg
    18481 acgagtaact gctccttgat caatctgcaa cttttcttgc aaactgcttt gtagcaatgg
    18541 attttcttct ttcagtctca tcatgatctc gtatctagta agacttatac cgatattttc
    18601 ttcaaagatc tggacgattt tttgatcggc taatttgatt tgatataaaa ggtgactaat
    18661 ctcttctaaa gacataattt cctcctttct aataattgac tcgtcaacga ttgacttgtc
    18721 aataatagaa taaaaaaaga aaaaagtcaa agaatcagct gtgtaataaa aaaaacgctc
    18781 tcaatagaaa agtatagaga aaagaaattt tttgtagaat gaacggaaaa aatgataaag
    18841 aaagcacaac gctaaaacca agattattga ttcttatcaa actatagaag atagctcttc
    18901 tgcagtagat aattttacac cgccaaaaat ttccgaaatc gccttttgca agaatcccgt
    18961 cgaatattag aaattggatc agtatgctta aacagtaata aaaaagttat gacaagatgt
    19021 tgtcacaacc ttttttgagt aaacggtgtt caaaaacttg catctgcggt aataagttca
    19081 accaaacaaa aaacatgaaa atctgttttt gtttggctgt tcttactact tgatgcaggg
    19141 cagctttttc actacctctt tttaataacc ataaaatcct attccttcat atctccagcc
    19201 catcggtgtt agccaatctc tttccttagc actcaaggta tagtgatggg taccagtttt
    19261 ttcatttgga ttaaatagtc gataaattgg cttggaacca ccagaataaa aacttagacc
    19321 ttcataacgc caaccgattt ttgttaaaga gttgatctca gattgtgcca ttgtgtaatg
    19381 atggtctcca ctgtaaggat tgtataaacg gtagacaggt tgtccgctgt ttggtgcttg
    19441 ccaagcaatc ccttcatatt cccagcctac agttggcaga taattttttt ctccggcatt
    19501 caatgtatat aaatgttctc ctgtatgacg gttatacaga cgatacatcg gagaagttga
    19561 agttggtgct gtatcatact gtgtcagatt ataggtttcg attacattgt tcaatgtaga
    19621 accataatta ggcgcagtgg cataacgacc tgttagccaa gccgtagcat ctttgtaaga
    19681 gttcgtattg cttttccatg caccggaata gtagtaaacg cctggctgaa aagaagtcgt
    19741 cttcaatacc gttacgttat cttgcaatga ttctttgtac gaaggatatt ttctaaaagc
    19801 ggcattcacc gtaatccatt gcccattcac atattccgaa gtaggcatcg taacagattg
    19861 gccattatag cttcctttaa tcccaaatag attgtaatta ggggcagaag ccaaagtact
    19921 gttcccccag ccactttcaa cgatggcttg agcaatcatg actgatgcat ataagtcatt
    19981 ggcacttgcc aaactttgtg cagttgaggc aatactatca atgaacgctt gttgagaatt
    20041 agcagatgag cgagcataaa aagcatagcc agtatagttt tcgaattcag acaaatctgc
    20101 ttgatgggta tgtgtcaatg tttgcccatc tgtcaattgg ttcgtgattt cttctgcatt
    20161 ctctggattg ttctgagctt ctgctgatgt actgtcttcg gtttgtgttt gttctgttgt
    20221 ttcagttgtt ggttcttcgg ttgaaattgt attttctgag acagtagctg actgcaaagt
    20281 actttctgta tcgttttcaa ttcctgtttc tgaagaactc gatgtctgag ttgcgacagt
    20341 ttctgtcgat tcttcagcta gcactgttgt gttggcaact gctagatttc ctaaaagaat
    20401 aacgctaaat aataaattct ttgctttcat cttttttctc ctttgaatta tgaaactttt
    20461 cctaaataac agtgtaacta aaaaagtaaa agcaagtcaa tgtccatatt taaataacat
    20521 ttgtttcttt ttaataaatt tgctttttct gcataaaaag acgtaaatga agaatattct
    20581 tctccattca cgccttaaat tgatggtcgt ttgaataact atttaacaag taattctctc
    20641 tggatattat ccgttatgga ttatacttcc tgtgtcgtcg gacggataat catgttgctg
    20701 acagacatcc ggtctggcgt ttctatcgca aatgctacag cagaagctat atcttcagat
    20761 gttaatcccc attctttttg cgcttcatga agttcaagag cagccttttt gtcgctgatg
    20821 gtttgatata attcagtttg aacagctcct ggagagatga tcgtagattt gatgttattt
    20881 tgccgttgct cttgacgtaa tccttccatg attgcacgga cagcaaattt tgttccgcaa
    20941 taaacggcgg aatcaggata aacaacatgt cccgccacag aatcagttga aatgatatgc
    21001 ccagattttt gtttttccat gattggaagt acggcggaaa ttccatttaa cacacccatg
    21061 atgttgatat caagcatatt ttgccattca tcacggtgac cttcaactaa tggagcggtt
    21121 ggcatgattc ctgcattatt gtacaataca tcgattcgac cgaacttttc catcgctaaa
    21181 tcgataactg cttggacttg tgcaaaatct cttacatctg ctgtttgaat atatagctca
    21241 gcttctggta aagattcttt tattgctttt aggcgatctt ctcggcgtgc agctataact
    21301 agtttagccc ctttttctgc taatagcttc gttgttgctt caccgattcc gcttgaagcg
    21361 cccatgatga caatcacttt ttctgataaa tcattcattc tattctcctc ctgttttgaa
    21421 aaatctcttg cttatactca ctatctctta tttcatctta ctgttcaaat aatccgttcg
    21481 acaggtttga cgattttggc agggattcct gcaacgatgg tattgtctgg gacatctttc
    21541 gtaacagttg cattcgctgc aacaatggca ttctcgccaa tcgttacgcc tggcagaata
    21601 gttgctcctg ctccgatcca agcatttttc ttgatgtgga ttggctttac tgacaagcct
    21661 cttcgttctt ttggatcggt tagatggttc accgtgatga ggttgacacg aggaccaatc
    21721 aatactgaat cttctaaaca gatgccacct aagtcaacga acatcacttg acgattgata
    21781 aagacatttt tccctatact aatatgtttg ccgaaatctg tataaaaagg cagcagaaca
    21841 gtcaccgtat catctacttt gtcactaatg atattttcta gccgtttgcg gatatctgct
    21901 ggttcatggt acccagtatt cagcgctgct aaaggctctt cgttttccgc ttggatctga
    21961 tggatcactt caaagagcgg atccgttact cgaatctctt tattgacgat ttgtttttcg
    22021 atgaggcttg tcattttgct ccctcctttt atccttactt tataacggac ggcttatgat
    22081 gtctaatact tataaaacat acttaactat tccttttaac tatacttagt ggtatgcttt
    22141 tttaaaaaca ggagggaaaa aatggatatt cgtatactga cgtatttttt agcagtagct
    22201 agagaaaaaa atatcagccg agcagcaaaa tctctgcata tcacgcaacc tactctctca
    22261 aaacaactga aagatttaga agaagaactt ggtgtgacac ttttcactcg aggctcccga
    22321 gagatacagt tgacggattc tggtcgatat ctttattcaa aaggaaaaga aatcttgtcg
    22381 ttggtagaaa aaacggcgaa caatttaaca aatgacgcaa tcatctctgg agaaatctac
    22441 attggcggag gcgaaactaa agcaatctct ttcatttctg atagccttca ttcgttgatc
    22501 aaggcgcatc ctgatatcca gtttcattta tatagcggga atgcagatga tatcatagac
    22561 aaactggata gcggattact tgattttgga ctcgtaatcg aaccgacaaa caaacaaaaa
    22621 taccagtcat ttcgactgcc agcccaagat cgttggggac ttttgataca tcggaatcat
    22681 cctttagcaa agcaaacagt catccatccg caggaactga aaactgttcc tttgatcatt
    22741 tcccggcaaa cgtcagtgga cagtcaacta gctgaatggc taggtttcag catagatcag
    22801 ttatcgatcg tcggaacata taacttattg tacaatgctt ctttgatggt cgataacggg
    22861 acttttggtg cattagctct agatgggatc atcaatactt atgggacgga aaaagtattc
    22921 atcccactcg atccgccatt acaagcttca ctgaatctca tttggaaaaa agaccagcct
    22981 ctttctcctg ctgctcgtaa atttttagat gtcctaacgg agaaatacag ttaagtagag
    23041 tttttttcct tcgctttcag ctttttaaca acgaataagt taagcaaaag atccaatgat
    23101 taaataacat aagtttctta atttgttcct tccttttatt tctgataatt ccagtaaatt
    23161 actataatta taaaaaaaga attgacaaaa cataactgtc tatataaaat tgattacgtt
    23221 aacaataatt cgtttcatgt gactagatca tactaggcat gaaaaatctc tgaggaacat
    23281 ggtttcccta ggaggttttt cgtgtctatt tttgtttggc cgcaatcaac tggtctagca
    23341 aacgtgaaaa ccaaatcttc aaaaagggtg cgcgcatgaa aatcaaaaag atctttaatc
    23401 aaaatgcagt attggtagat gacggtggac aggaaaaagt agctattgga aaagggattg
    23461 gatttgacag aaaaagaaat gatttgattt ttgatcgtga tgtcgaacgg atatttgtca
    23521 tggaaccaga aggtcaaatc aagttgcaaa atttacttaa tcagattgat gaacgctttt
    23581 tatttgcagc agaaacgatc attgatcatg cggaaactgt tttgatggaa aagttgaatg
    23641 aacatgtatt gatcgctttg accgaccaca ttgctttttc tgcggagaac attaataacg
    23701 gtatcgtcat ccgcaacaaa cttttaagag aaatcgaagt gctttacagt gatgaattca
    23761 gtattgctca atgggcagta gagtatctga caaaagagct tgggattcca tatacttatg
    23821 acgaagcagg atatatcgca atccatattc acagcggacg aagcggtcgg aacaataacc
    23881 atcgcagtat ccgtgaagtg acgattgtat ctgacatcat ccatttagtg gaatcagaat
    23941 tggaaacaga tatccattca gaaccatttt cactgaatta ttcaagactg gtgaatcatt
    24001 tacgactgct tctacaacga acccatgcgc aacaatatgc tgttttagac acggaaatcg
    24061 tagaaatggt caaacgaaaa tacccagaaa gctataagat ttctaaaaaa atccgtgtgt
    24121 tgctgaccaa agaataccag ctcgcgatca cgaaagaaga actgggttac ttggccatcc
    24181 atatcgagcg tttaagaagc gcaatcgtac aaacaaatgt taacaatcat gaggaggaaa
    24241 aaaattgaaa gcatatctac aacgaatggg acgttccctt caacttccag ttgcggtctt
    24301 acctgctgct gctttactag tagggatcgg aaactggtgg gcatcttata gtaacgacat
    24361 tgtcgctcat tttttacaag ctggcggtaa cgcagtttta ggtcaattac ctttgctgtt
    24421 tgccgttggg ttagcactag ggatgtcagt ggacaaatca ggtgctgctg ccttggcagg
    24481 tttggtcgca tttgaagttc cagtaaatgt tttaaaaact gattcagtag ctactttatt
    24541 gaatatcaaa ccagaatcag ttgatccatc ttttgctcag atcagtaacg tatttattgg
    24601 tattattgcc ggattgattg cggcagctct ttataatcgt ttccacgaag caaaattacc
    24661 gatggcttta tctttcttta gcggcaagcg tctagtgcca atcttggctg cctttgtcat
    24721 gttgctgatc agtgcagtgc tactgctcgt atggcctccg gtttactctg ctttagtttc
    24781 ttttgctaaa ttcattttga gtttaggctg gggtggtgca ggtctttatg gcttcttcaa
    24841 ccgtttactg atccctacag gattgcatca agcaatgaac tccgtgttct ggtttgacgt
    24901 agcaggaatc aatgatattg gtaaattttt ggcttctgaa ggaacaaaag gcgtaacagg
    24961 aatgtatcaa gctggatttt tcccaatcat gatgttcggg cttccagcag gagcatatgc
    25021 tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca gcttccttga tgttagcagc
    25081 agcttttgct tcattcttta ctggtgtaac agaaccactt gaattctcat ttatgttcgt
    25141 tgcttggcca ttatatgtct tgcatgcaat tttcactggg atttctttag cagtgagtgc
    25201 attcttccat tggacagcag gatttgcctt tagtgccgga tttgtcgatt acttcctatc
    25261 tttgaaaaat ccgattgcga atcagccatt gatgttgatc gtccaaggat tagtaacagc
    25321 agtgatttac tactttggct ttaactttgc tatcaaaaaa ttccatctga tgactcctgg
    25381 ccgcgaggaa gcagatcttt cagatgacga tactgcaacg actaattcag acaataaata
    25441 tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga gccgacaatg taacttcgat
    25501 cgataactgt actacacgcc ttcgtttgca agtcaaagat acaggcctga tcgatcaaaa
    25561 taaaatcaaa gccactggcg tacctggtat gaagatcatc gacggaaaaa atgcgcaagt
    25621 catcgtaggt acagaagtac aattcgttgc agatgagatg gcaaaactac atggtggtgc
    25681 tgcaacacga cctgcacaaa caaacaccgt ggttaaaaca gaaacatttg ccggtggaac
    25741 gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg attccaatca ctgaagtttc
    25801 agatgatgta ttcgcggaaa aaatgatggg tgacggctat gctgtattac cagaaaatgg
    25861 agaaatcttc tctccaatcg ctggtacgat cacaaacatt ttcccaacga aacatgctgt
    25921 cggaatccaa acagatgctg gtatcgaagt cttgcttcat atgggcatca acactgttga
    25981 cttaaaaggc gaaccattca ctttatatgt aaaagaaggt caaaaagtcg caagaggtca
    26041 attgattgct ttagttgatc ttgccgctat tcaatctgca ggcaaaaaca cggatatggt
    26101 cgttgtattc acaaatggcg acaaagtcca aagcttagaa atcgaacccg cacgtgatgt
    26161 aaaagcaaat gataaaatcg gctctgtttc caacaaagcc tgagccatcg tcttccccag
    26221 acttttttat cgataaacaa acaggagctg agacaaaaat cttgtctcag ctcctttttc
    26281 cgaataaaaa cggcgttctc ctgccaactc cgcggtaata agtccagcca aacgtaacat
    26341 atgaagagct attttttatt tgactcttct tattacttga tgcagaacag cttttttaca
    26401 acctctgata aacggtgttc aaaaaccagt ttatcggtat gaagtcaaat ttatgcaaaa
    26461 atgtggaaag ccattttcac aaaatttttc ttcatactca aaactaatcg gctttttcac
    26521 aacctcttta tacattattc ctgatccaat atttctttta atgctgcttc aagagaagtt
    26581 aatggttttc ccaatacttt ttctaaatca tcagaggaaa tagctaactg atccttttta
    26641 atatcatgtt ggatcgctaa aaacatgtca gctgcttctt gagggaatcc ggcttctttt
    26701 agattttcga caaacgctgc atcacttgct tcaataattt ctaacttttt acctgtcgca
    26761 cgctctaatg ccttggctaa ttcttcatag tcagttagat tgccggataa ttctaagatt
    26821 tctggagagt cactttctac tagtactttt gctgcagctt ctgcatattc acgtttcaat
    26881 gcccaacctg tttttccatt tcctgcagca taaacgaatt gaccagtttt taaagcctgc
    26941 ccaatgatag gtaattcatt ctctacatac caattattcc gtaaaaatgt atgggaaata
    27001 cctgaatcct taatcatttt ttcagtgaat tggtgatctg gcgctaatac actgatcgag
    27061 tgatcggcat ctgcaaaact tgtataagcg atataagata ccccagctgc ttgtgctgcc
    27121 ttcactacat tcttatgttc ggcttgacga tttcctggaa cgccagagac aaaaagcaat
    27181 cgatctattc ctttcaacgc ttctttcatt gagtctaaat catcataatc accaatacgt
    27241 atattaaatc cagcttcttt cagtgcttct cctttttctt cactacgtac tagtacatag
    27301 atttcatttt gtggaaccat cttttttaag aattccagcg cataaccacc aaaacctcca
    27361 gttgctcctg ttactaaata atgcatattt gccactccct attcttttta tttgttaaga
    27421 tttacagctg tacatttata aattacacct ttatcataaa gagtgtggta agataagtca
    27481 agcaaaaaaa atgaaaggat gacaactgat gaagtattct ctgcaattta gtgatgcaat
    27541 ccatgtactt gcctatattg aaatgtttca agatacccga tcactttcta gtgagatgat
    27601 tgccagtagt atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc agctcaaaaa
    27661 atccggtttg atctttacaa aaaccggaaa accgcagcct tcccttgcca aaagtccaga
    27721 tcagatttct ttactagatg tctatcgcag catcgaaggt aatacacagc ttatccaagt
    27781 cgatccaaaa acaaatccaa attgcgtcgt tggtgcgaat atccaagaag ctttagaaga
    27841 aagctacgct cggctgcaga aaaaggcaga agaagaaatg gctaagatca cacttgatag
    27901 tctgatacat aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca caggcctcgt
    27961 agagagattt ttataattct ctctactgtg cctgcatttt tctgatcgga atcgtctaca
    28021 taaattcttt tattctccgg gggacaaaag ccttgtcatg cccctttttc ctctctaatg
    28081 ctcaggtcaa agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt agctgaccct
    28141 cggcattagt tctgaaaaat gcaaaatatg gaaagctatt tcccattttt cctctctaat
    28201 gctcaggtca aagcgctact ttcacgacct ctatattatc atggtttatt gactaccggt
    28261 atccagattt ctcccatata tttcccgttg gtaactccca tttcgactac agcatttgga
    28321 cccccaacat aggcgtaatc ttctacttct cttaaaacat gcccaaaggc cgtattcgtc
    28381 aaatcatttc ttaattcttc aggcgattct gctaaagcga ttacaacaag atacgttcct
    28441 tttgggaatg ggatgattcg ggtagtgttt ggaacatcct gactcgtttg aacaccgaca
    28501 tagtacatca ttttcccgtt atagggttcg ttgactgtaa agaaataatg attttcagca
    28561 gcttgtttca atgcatctat tgttccatta ccgataactt tttgctcaaa tgcttgtttc
    28621 tctgcagcca aaccgacaaa atccgtattg ctactcttca attccacacc aatacccgtc
    28681 accgtaaaat ctggtttttc ttcgatccta tactttgcca tttctcccac cctttctatt
    28741 atattaagtt catcttacat aaaattaaag ggaaatgaca cgattacgct cttttttcta
    28801 gggatatttt tattttttaa ctattttttt gctttcactg caatccaaat ctcactgtaa
    28861 acattactga aatcctctcc ccattttgta aaagagatct cgggagcttc gactaactca
    28921 taatctgaag aaggaagcca ttctgcatag attttccccc aagtatcttg taaagtttgg
    28981 ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt cttctatagc aagcatttct
    29041 aaatcagcgt acggattttc ttttgtcgta gcaaagccaa tcatgtgcat caaactgccc
    29101 ttttcttcca agcgggctcc atcaaatcca taagaaacgt tgattacttg atttggatat
    29161 aaatctccta acttatgcat ctcttccctt tgagtctccg tgatcgtctg cgctaacttt
    29221 tgtatttctt gattgactcc ttcaaatttg acaggaactt ttttcgccac acctgccaca
    29281 taaaactgtt ctttcttttc gatacgaaat tccatcgatt ttcctcctct gacatcaata
    29341 aaaaatgtca agcgtggaaa agatttttgt atgccgtatc tgctgatttc tgaaggcaga
    29401 tatcctgacc aatcacgaaa agctcttgaa aaaccttcga ctgtttcata tccatatcta
    29461 aacgccgttt ctgtcactct tgatccatct aggagatcgc gattagccaa agccaatcta
    29521 cggtttttga tatagtccat tagcgacatc cctgcaataa acgtaaagat gcgttttaat
    29581 tgatattcag agattcctgt attttcggct attattttat aggaaagtcc ttctgtcaaa
    29641 tgttcttcga tgtaatcgat caattggttc agatctttaa gcatatttat cttcctcctt
    29701 gcttgccttt ttatcatatt atcaaagaaa gtcaattact tgatgaatca ggaaccaatt
    29761 tagtcagatg tagaaaatgt tgtatggatc taaagaaaat tctcaagcag cgtttcgtaa
    29821 cggataattg gtattatctg cctatctatg ttttaccttt agaaaaataa attttgggga
    29881 agacacattc taagtaaaca aagcggtaaa aatatctttt attcccttag ttaatccgct
    29941 tactacctac tgtacaaagc aaattgttgt tcaaactgct gatcagaacg ctgcttggat
    30001 ttttctttgc gatacatcat acctctttta tcaattggaa caattattta tttctaacaa
    30061 aatcacttca tttttgtcag ataaataaaa aacaaaaaaa gttccatctt taaaacacgc
    30121 tattctttta ctttttctca cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa
    30181 ttcgagcaaa agtgattatc aaatatgtaa aatcagctac ttttttctta aacgtttctt
    30241 tatactaaat ataaataagt gtttcatgaa aggagaacta gatgaataat tggcttgagt
    30301 attttcctga aaacgttttg gaacgcggtt acagctatca tctgcacggt tttgtccgtc
    30361 atttgaacta tacaagcaag tacttgtccg caacagtttc tggcacggaa gattataagg
    30421 tagtgatcac ttgggatgaa aagaccaaca tgacttgtga ttgtctatat gccatagaag
    30481 gaaaaaaatg caaacacatg gctgctgtct tattcgctta cgaagaacgt ccaataaaaa
    30541 aatccaacta ctcactttcc gaactctcgt cccttgtttc ctccgctagt tcctctcttg
    30601 tacgggagct tttaacagag attcttatag aacatccgca atttatcgaa cgcttcaaag
    30661 ttaagatgcc atttcatgca ataaattatt cagataaatt aacaactatc atccataaat
    30721 acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc atggagatgc
    30781 gaaagttcat ccaagaagca gttgaatcgc tcattcagca aaatgcctat cttcccgcat
    30841 tcgaattgat caatgaagtg attgctactc tcgaaacatt ctattgggag cctgaggatg
    30901 aacgaaccct tcttctgata gaggactgtt attatttatg gaaagaatta ttagcagaag
    30961 cacctcatgc agaaaaaagg caaatgttct cttggttcgt ctgccaagtc gatcatactg
    31021 acgcctctta ttcaaaacgc tactccataa aaatcctgaa agaagacttt cgggaaaaag
    31081 aattttctaa ccaaaagaaa aaaatagata aaaaaactaa agaaacggta aagaaagacg
    31141 atttcaatga aaaatagcaa gtaaacgata acaaccatcc taaatccaca agaaatacag
    31201 cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt
    31261 acccaatatt agtcatttga ctattaggta cgcgttttgt tttataagtc atgacaaatt
    31321 tccttcgttg ctgggtagat tttccggcat tgttgataga ttttatgata cttttctaca
    31381 ttttgtaaga gaggttccac tgcatctgta tattggataa aattctgaca acaactttca
    31441 ggtgtgtcga accactctaa tctttaaaga taaatattaa aataacgatt aataatatta
    31501 acagtcttta tttttcacca ccaaaaacct atctttgcat atactaaata gacgtatata
    31561 cacagatagg tttgtcttgt gaaaaataat gattatcctt accaaaaaat gtttgttttt
    31621 ctacatgttc gtacttatca aaaaatatga tccttattga aattactggc tatttatatt
    31681 ccaacgactt cttgtgttaa tttttgttat ctcatagtaa attatgtttc attttcactt
    31741 aatttagttg tcacgtgttc gtcaattcgt aaaaacggta aatatataaa cacacttgcg
    31801 gctataatta atatttgtaa aacagctgct cgccagtctc cggctgttgc taaaaatccc
    31861 gatataactg gtggcgtagt ccaaggaatt tgcactacta catgattaat taatcccaca
    31921 gcagttgcaa aatatgctac tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc
    31981 agactgatat taaatacaat aggcaatcca aaaataatag gttcgcttat attgaatata
    32041 gcgggagtta ccgataattt agcaaagtcc ttataatctt ttctacgact gaagataaat
    32101 attgcaatca ataaagcaat cgtatttccc gatccaccca ttaccgcaaa ggtatcttta
    32161 aaggccatat tgataatatg tggaatttgc tctttgtttg cataagctgc catattttct
    32221 tgcatattct gaatcaaaaa cggatctaac aatgaacctg atattactgc ttgatggata
    32281 ccaaatccaa aaaatagatt agcaatagat gtaattaaca aaaatcctgg taaaccagtt
    32341 gtaattttac ttaatggctc tgtaactatc gtggtaatta gcttatttat atccatatta
    32401 aacaattgaa tgattataaa tgacaataaa gcaaacgagg taatcgtaat aatgattggg
    32461 attaatacat taaaagactt taaaacagcg ggaggaatat ttccggatag ctgaatctgt
    32521 agtttcttat tattagaaag tttaataaat aattctgtag ccgctaatcc aacaaatata
    32581 cctacaaaca ttcctgaagc tcctgtatag gatataggta tcacacccgt cacttcaaca
    32641 gctttatccg cattttcagg agtaaacatc atctgcatag gtgttaatac aacaaatgtc
    32701 gaaatagcaa ctaataccgc tgcaatatga tttgtataat ttctgcttgc acacatatga
    32761 taagatactg ctgctacaac taataatgta gttatgctta atgtaccatt ggcaattgaa
    32821 gtaccaattt gttgtaacgt ggtcaatgta ctagtatcga caatttcccc catgaatcct
    32881 gttggttcta agatcactga attaataagt gtcacaaatc ctgctaacat cataaatggc
    32941 ataaatgtag caaacgcatc tcgcaaagtt cttaaatgga tctgattccc caacttagtc
    33001 gcaactatac caaatttttc aaatacaatg tccttcctgc tacttttgcc catatttttt
    33061 ctcctttatg cttattgtaa tcgattactt tttccaccca aataatacat cacttatcaa
    33121 aaaaagtaaa
//
LOCUS       DAPCMT010000016        32169 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPCMT010000016 DAPCMT010000000
VERSION     DAPCMT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.016"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(107..487)
                     /gene="yajC"
                     /locus_tag="RD743_000621"
     CDS             complement(107..487)
                     /gene="yajC"
                     /locus_tag="RD743_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDU0562477.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(584..1729)
                     /gene="tgt"
                     /locus_tag="RD743_000622"
     CDS             complement(584..1729)
                     /gene="tgt"
                     /locus_tag="RD743_000622"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDU0562478.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1947..2645
                     /locus_tag="RD743_000623"
     CDS             1947..2645
                     /locus_tag="RD743_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDU0562479.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2813..5452)
                     /locus_tag="RD743_000624"
     CDS             complement(2813..5452)
                     /locus_tag="RD743_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDU0562480.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6071..7102)
                     /gene="queA"
                     /locus_tag="RD743_000625"
     CDS             complement(6071..7102)
                     /gene="queA"
                     /locus_tag="RD743_000625"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDU0562481.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7295..8470
                     /locus_tag="RD743_000626"
     CDS             7295..8470
                     /locus_tag="RD743_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDU0562482.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8474..9115
                     /locus_tag="RD743_000627"
     CDS             8474..9115
                     /locus_tag="RD743_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562483.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9112..10029
                     /locus_tag="RD743_000628"
     CDS             9112..10029
                     /locus_tag="RD743_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0562484.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10026..10697
                     /locus_tag="RD743_000629"
     CDS             10026..10697
                     /locus_tag="RD743_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562485.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11238..11726)
                     /locus_tag="RD743_000630"
     CDS             complement(11238..11726)
                     /locus_tag="RD743_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDU0562486.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11927..12193)
                     /locus_tag="RD743_000631"
     CDS             complement(11927..12193)
                     /locus_tag="RD743_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDU0562487.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12193..12456)
                     /locus_tag="RD743_000632"
     CDS             complement(12193..12456)
                     /locus_tag="RD743_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDU0562488.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12932..13117
                     /locus_tag="RD743_000633"
     CDS             12932..13117
                     /locus_tag="RD743_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDU0562489.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13259..15082)
                     /locus_tag="RD743_000634"
     CDS             complement(13259..15082)
                     /locus_tag="RD743_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDU0562490.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15680..18385)
                     /locus_tag="RD743_000635"
     CDS             complement(15680..18385)
                     /locus_tag="RD743_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDU0562491.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18691..19638)
                     /locus_tag="RD743_000636"
     CDS             complement(18691..19638)
                     /locus_tag="RD743_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDU0562492.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19828..20097
                     /gene="rpsN"
                     /locus_tag="RD743_000637"
     CDS             19828..20097
                     /gene="rpsN"
                     /locus_tag="RD743_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDU0562493.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20273..20635)
                     /locus_tag="RD743_000638"
     CDS             complement(20273..20635)
                     /locus_tag="RD743_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDU0562494.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20646..21767)
                     /locus_tag="RD743_000639"
     CDS             complement(20646..21767)
                     /locus_tag="RD743_000639"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDU0562495.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21783..22133)
                     /locus_tag="RD743_000640"
     CDS             complement(21783..22133)
                     /locus_tag="RD743_000640"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDU0562496.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22263..23774)
                     /locus_tag="RD743_000641"
     CDS             complement(22263..23774)
                     /locus_tag="RD743_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDU0562497.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24096..25448)
                     /locus_tag="RD743_000642"
     CDS             complement(24096..25448)
                     /locus_tag="RD743_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDU0562498.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25601..26677)
                     /locus_tag="RD743_000643"
     CDS             complement(25601..26677)
                     /locus_tag="RD743_000643"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDU0562499.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26873..28618
                     /locus_tag="RD743_000644"
     CDS             26873..28618
                     /locus_tag="RD743_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDU0562500.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28898..29335)
                     /locus_tag="RD743_000645"
     CDS             complement(28898..29335)
                     /locus_tag="RD743_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDU0562501.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29319..30692)
                     /locus_tag="RD743_000646"
     CDS             complement(29319..30692)
                     /locus_tag="RD743_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562502.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31014..31502)
                     /locus_tag="RD743_000647"
     CDS             complement(31014..31502)
                     /locus_tag="RD743_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0562503.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31524..32126)
                     /locus_tag="RD743_000648"
     CDS             complement(31524..32126)
                     /locus_tag="RD743_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDU0562504.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9195 a         6878 c         5940 g        10156 t
ORIGIN      
        1 tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg
       61 taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct
      121 tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg
      181 gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga
      241 ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg
      301 tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg
      361 aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg
      421 aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg
      481 ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg
      541 ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt
      601 tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat
      661 ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag
      721 aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg
      781 tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat
      841 atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga
      901 ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg
      961 aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag
     1021 tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg
     1081 agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa
     1141 accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca
     1201 atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt
     1261 catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga
     1321 tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat
     1381 gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg
     1441 aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta
     1501 ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac
     1561 ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt cctactggca
     1621 tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag
     1681 tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta
     1741 ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc
     1801 aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga
     1861 ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg
     1921 ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa
     1981 aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga
     2041 ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc accaccatat
     2101 atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg
     2161 gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt
     2221 atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg
     2281 gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta
     2341 caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc
     2401 aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg
     2461 atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag
     2521 atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat
     2581 ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata
     2641 tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag
     2701 gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca
     2761 cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct
     2821 cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga
     2881 tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt
     2941 cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt
     3001 ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat
     3061 tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat
     3121 gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa
     3181 acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag
     3241 caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct
     3301 ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca
     3361 cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg
     3421 aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc
     3481 cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg
     3541 cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca
     3601 acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc
     3661 aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt
     3721 tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt
     3781 ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc accacatgat
     3841 ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac
     3901 tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca
     3961 gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca
     4021 ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg
     4081 aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc
     4141 ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc
     4201 gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga
     4261 tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca
     4321 catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt
     4381 cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg
     4441 tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg
     4501 gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca
     4561 acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca
     4621 tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag
     4681 ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg
     4741 ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc
     4801 caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca
     4861 tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa
     4921 ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca
     4981 aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca
     5041 gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat
     5101 tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata
     5161 ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg
     5221 tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat
     5281 cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat
     5341 tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat
     5401 ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc
     5461 tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt
     5521 tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta
     5581 tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca
     5641 aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag
     5701 gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt
     5761 tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa
     5821 cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa
     5881 aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac
     5941 aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct
     6001 cggctttttc tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct
     6061 tttcataacc ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt
     6121 caatcgcatg ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca
     6181 tgactaaagt cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt
     6241 catagccagg cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa
     6301 atttcgttcc aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc
     6361 cgccattttt acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt
     6421 cactatgcat atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc
     6481 ctacatgtag tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt
     6541 ctttcgtaaa atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga
     6601 cagtttgata gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt
     6661 ctcccaatga ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc
     6721 cgccgtgttc caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc
     6781 ttgtcccaac tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg
     6841 tattcgttag aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac
     6901 gagcaggcag aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa
     6961 tgatttcatg gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac
     7021 gagaatgatc gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat
     7081 caaaatcttc tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta
     7141 tcatgttttt cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct
     7201 aaataaaaaa actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata
     7261 gttcttcatt gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa
     7321 aatttataaa ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga
     7381 gttcatttgt tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa
     7441 ccgaatgact gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat
     7501 tgatccagtg gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc
     7561 tcacatgacg atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga
     7621 agaacggaat aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt
     7681 agatcgctac ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc
     7741 ccttgcagcg aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat
     7801 cactcgtgat tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat
     7861 cgtatttgta acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat
     7921 gagtgaaggc cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa
     7981 cgatttcgta gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac
     8041 aagagtcggc gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca
     8101 agaagcaatc aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc
     8161 gaatgtatta aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc
     8221 cagtgtcggc gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg
     8281 tgataccttg caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca
     8341 gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat
     8401 ttggggagaa gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa
     8461 ggaggtctga cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg
     8521 ttttgaacat ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc
     8581 gcttggtgtc ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag
     8641 tgccttgcag acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt
     8701 aggtaaatta cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa
     8761 tacgtacatt gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg
     8821 gatgactgag ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat
     8881 ggctggtatt cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat
     8941 cggcgctggt ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct
     9001 cattttggca ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg
     9061 tctcttggaa caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt
     9121 atactaaaaa tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct
     9181 ggtcttgcca gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa
     9241 gcacagatct tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc
     9301 gcaatcatca acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat
     9361 gcttcaattt cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct
     9421 cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac
     9481 ggtcaaacgt ggttcccgtc ctatagtttt gaaaatacgt atgtctttct tgttcgaaaa
     9541 gacaccgcac aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa
     9601 ttagtagctg gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc
     9661 caagaaactt atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat
     9721 gacgcagtcg aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc
     9781 gccagttatg atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct
     9841 gctccagttg tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat
     9901 aagttaggtc ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat
     9961 aatctagtgg aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa
    10021 gggaagtgaa gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg
    10081 aaaatggctc ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg
    10141 tcctctttgc agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat
    10201 tagcaaactg ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga
    10261 tctccatttt aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt
    10321 tgtattccct cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa
    10381 acatccttga tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag
    10441 agttaccact ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca
    10501 tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc
    10561 gaggaaccaa tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat
    10621 taatggcgat catcacggac tggcttttag gtattttgga aagacggcta gatccagcca
    10681 gccgtacatc acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc
    10741 gcaaaggctt ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc
    10801 ttatgggtaa agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata
    10861 ctgccaacgg gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat
    10921 tttgccaatc cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac
    10981 tcgtctgctt gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt
    11041 taagcctttt ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt
    11101 gactgccgta tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc
    11161 cttggctaaa aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta
    11221 aaaccggtac acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga
    11281 tccttcacca gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag
    11341 acattggcgt aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg
    11401 gccgaaagcg ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc
    11461 gctttggata cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct
    11521 tttccaaaag accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg
    11581 tccattgctt ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc
    11641 ttcgcctcac tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct
    11701 ccaattttac tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa
    11761 cattccattc ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa
    11821 agaaatcatg caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt
    11881 ggggagactc tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg
    11941 tccaggcaga ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat
    12001 gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag
    12061 actgcgtggg catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct
    12121 ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg
    12181 tatagttact catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc
    12241 gtctttttcg cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc
    12301 ttgaatcgct ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact
    12361 aataatctcg atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt
    12421 acgagcagtg ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat
    12481 aattatagcg tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca
    12541 aacttttcct cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac
    12601 gctcacttct tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg
    12661 tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca
    12721 cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac
    12781 gaaagatttg taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa
    12841 aaatgattgc ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta
    12901 gaccgttggg tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg
    12961 gtcacttgac gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt
    13021 aaaggaagaa gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta
    13081 tgatcaagac atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca
    13141 tacgctgttc ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa
    13201 cagaccctct agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt
    13261 atttcttcaa atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca
    13321 atcttaaccg caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa
    13381 attgaggaat caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat
    13441 ctacataacg gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac
    13501 ggacatctgg gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt
    13561 cgtctagcga aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg
    13621 caccgatatt tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat
    13681 catgtagttt tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat
    13741 ggatcttata aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc
    13801 ctaaccgata gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat
    13861 tggcaatcga tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg
    13921 tttgagataa agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg
    13981 ttgagccatg gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat
    14041 tggaatagcc tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg
    14101 ccagattgta tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa
    14161 tcagcgctgt agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac
    14221 caatttgaga caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa
    14281 atgtgatccc cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg
    14341 cattttttgc tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag
    14401 ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca
    14461 cagccgctcc tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata
    14521 aaccaacaac gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga
    14581 acgatgcaga ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca
    14641 cagaaatagc gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac
    14701 ctgctgagac agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa
    14761 tcaagccagc attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag
    14821 gataagcatg gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa
    14881 aagcggcaat gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa
    14941 cgatttgttc cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag
    15001 cagcaaatcg ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta
    15061 acaacctttt taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt
    15121 tatagtcgaa aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca
    15181 ataaacttta agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc
    15241 tttaatacag caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa
    15301 aggatagaac ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa
    15361 gagagatgta aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt
    15421 cacaacctct tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa
    15481 ttaaaaacat aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt
    15541 ttttacaacc tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    15601 acggcaaaat atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact
    15661 gatttcacaa cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt
    15721 tcgtcatttt ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa
    15781 acaaaccgat catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag
    15841 attcccgtaa caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga
    15901 atttccctga gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga
    15961 tgataatagc gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca
    16021 tcatgaacgc taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat
    16081 ctactcctag taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg
    16141 tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt
    16201 tctttcctaa atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca
    16261 gacatagtgc agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg
    16321 cattggtttg tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt
    16381 tcaataattt gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg
    16441 ctgctttttg gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag
    16501 cgttcacagc tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga
    16561 tttcattttt tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt
    16621 gctgaatgat cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga
    16681 tggtatccat atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag
    16741 gcatttcttt ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg
    16801 agctgagaag cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc
    16861 caatcgtatc gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat
    16921 tgataccagc aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt
    16981 catcaatagc cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt
    17041 gcagattgct tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg
    17101 tatttccagc acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt
    17161 gttgttgaga tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat
    17221 catcagtcag cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg
    17281 ttgttgccac ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta
    17341 tcgcatcttt cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat
    17401 tttctaaatt tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt
    17461 gttttgcttc atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga
    17521 tctctgctag ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga
    17581 tgatttttgc ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac
    17641 ccatctcatc tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct
    17701 tgatttcagg tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt
    17761 ccaaattttc atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg
    17821 tatcgatgtc gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga
    17881 taaattcatt tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg
    17941 caatcgcatt gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac
    18001 tagtaaaact cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact
    18061 ttccggaacg gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt
    18121 gtttattttt atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa
    18181 actctgctgg cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg
    18241 gatcccataa tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga
    18301 tgatcagaag tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc
    18361 gaagcgtatt tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac
    18421 tttaccatgt atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga
    18481 catatcacgg caattgtctc attgtaatct cttacatatt gagaaataat taaggattac
    18541 agcctttact ttatcatgaa ataaaaggga aagataattt tatgccaaag tttttatcta
    18601 ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc
    18661 agcttttttc gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc
    18721 agcaggagga tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga
    18781 cagattcatt gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc
    18841 aaaggcttct gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt
    18901 tttttgccgc aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt
    18961 catgatctgg atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc
    19021 tgcctgatca gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc
    19081 tcctaatttt tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc
    19141 cttttcatgg agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat
    19201 tacagtatct gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc
    19261 tggcgtgacc attccggctg cttcttccca actcatttta tctggaattt ttgctacttt
    19321 tgctgatggc aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg
    19381 atcacctaca tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc
    19441 attacctggt acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa
    19501 ggcaacatca taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc
    19561 gacttctctg ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt
    19621 aattgcaaat cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgcctt
    19681 tattttgaca aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc
    19741 ttttttcgcg taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta
    19801 ataaaaaata attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa
    19861 aaacagcaag aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa
    19921 gattacgaag gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt
    19981 gatttaatcg atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa
    20041 ttccgtgaat tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc
    20101 aagtctggct gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa
    20161 gatacgttcg cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac
    20221 gcacagctgg tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag
    20281 taacaggagt cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca
    20341 taatgggtcg atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga
    20401 tctgctccaa caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa
    20461 tgtgagtcgg aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg
    20521 ggctaaaatg gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct
    20581 ccgatcccac gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat
    20641 cctccttatt cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag
    20701 tggatcgtac ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg
    20761 ccgataagcg tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga
    20821 tccatacata cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc
    20881 tgcatctttc gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct
    20941 tcaggagtga tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact
    21001 tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg
    21061 tgcccagagg gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt
    21121 tcatcatgcc ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga
    21181 agttttttca gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc
    21241 tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg
    21301 atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca
    21361 gtgtctgctt ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca
    21421 gctgctgctt gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct
    21481 acagtcagtg cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct
    21541 tctctcaact cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca
    21601 actgctgcct cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca
    21661 aacaattctt ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct
    21721 tttttatgta tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct
    21781 ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc
    21841 catacattgc cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa
    21901 atctcgacat cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc
    21961 tctttgcaag cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg
    22021 aatagtttca tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc
    22081 agccgagtga tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt
    22141 tctcttttca ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa
    22201 ataagtccac aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat
    22261 gtttaatcgt tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg
    22321 ccatcttttt ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa
    22381 ttaccgccgt cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat
    22441 ccttttttct gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct
    22501 ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct
    22561 aataatttat ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg
    22621 atttgttcaa ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt
    22681 aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt
    22741 ggtgtaacga aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacgg
    22801 tgaacgtagc tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa
    22861 atatctaatc cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta
    22921 aatgaacgca atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca
    22981 cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg
    23041 aagacgattg ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt
    23101 tcgtattctt ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct
    23161 ttgatcttca catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc
    23221 ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt
    23281 tcgatatctt ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt
    23341 tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta
    23401 cccacaacga tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg
    23461 ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt
    23521 gtttggatag ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct
    23581 ggatcgattt tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt
    23641 gcttgtccaa taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc
    23701 gttgcttctt caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt
    23761 tctttaaatt tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc
    23821 taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt
    23881 ttttatccat tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat
    23941 tagaaaagcc aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa
    24001 gttgtttcta tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta
    24061 acatagtttt tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct
    24121 accacttcaa ataagcccat gctgttgctg ccttttagca taatactatc tgttgcttca
    24181 agattttgtt tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt
    24241 agttgggggt atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg
    24301 aaaatcatgg cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa
    24361 gactctttcc ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc
    24421 agatttttaa aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg
    24481 ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa
    24541 acatgggcga gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta
    24601 tacgcaatca aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct
    24661 ccttctacta caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg
    24721 ccatttccaa taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc
    24781 aacggttcat ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc
    24841 attttggctt tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc
    24901 attgtgattg ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg
    24961 tgatccatgc ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg
    25021 taaggaagac caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca
    25081 gacaagacag acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca
    25141 acgacttctg cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa
    25201 ggatctgcta caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa
    25261 aatgaagcag atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta
    25321 ccagcaagag gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg
    25381 gtaatctttg tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt
    25441 agattcattg agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg
    25501 gttcgcatga ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa
    25561 cgcggttacg caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa
    25621 aagattgacg ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac
    25681 cgtatttcaa gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg
    25741 gcatagagtt taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc
    25801 ggctcaatcc gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt
    25861 gataaacttc ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatatt
    25921 ttgcttcata atcatagaat gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa
    25981 cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga
    26041 ttgctcgaga atcatactga taggctgttg ctaaagcatt ttgcagctct tctcggtttt
    26101 ctgcttttgt aatgccgaca ctagaaccca tattcgccgg tttgacaaac atcggataaa
    26161 gcaaagaacc ttcacattga tcaaatactt ttttaggatt ttctttccat tgattcttaa
    26221 gtactggtac ataaggaact tgcggcacac cagcagcttg taaaatatac ttggtcatga
    26281 ttttatccat ggcacatgca ctggtcaata cgcctgcgcc gacataaggc atattcaatg
    26341 tctctaagaa gccttggatc gttccatctt ccccgtttgg cccatgtaaa actggaaaaa
    26401 cgatggctcc ttcttctttg atttcgcccg gattgatcac ttttcctgta aagccttcct
    26461 ctgtctgtcc acttgggtcc catgaaagat gcaagacatc tttgctggca ggtttttctg
    26521 ttagtaatgg acctttgacc cattgtcctt cttttgtaat aaatacgagt tgaacttggt
    26581 aataattata ataaatggca tttaaaactg aaaatgcgga aagaatggac acttcatgct
    26641 ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa
    26701 atcgttcagt gatttactct catattttag cacaaaacaa aaataaacga ataacttatt
    26761 tataaaaaaa cgagccagct tctaaaagaa cgagcgtcat ttattttttg tcagctagat
    26821 tatttgtccc gttttttata aaaagttatg ataaagaaaa ggagggatga tgatggcgca
    26881 aacaaatcca cgcagccgct tggcagaaat cattcgagtg ttcatcaatt acaatgtagt
    26941 gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg aagaactagg
    27001 ccccaccttc atcaagatag gacagatgct ttctgttcga gaagatttgc tttcttctgc
    27061 cttcactcaa acattcaaaa cattacaaga tagtgtacct agcgacacat tttcaacggt
    27121 caaaaagacg atcgaaacag aactttctct ttcattatca gatatattcg atgatttttc
    27181 aaaatcgcct tttgcctctg cttctatggg gcaggctcat cgtgccaagc tgaagaacgg
    27241 tgacgcggta gtcgttaaga tccaacaccc taacatcgcc gaagagatcc gacttgatct
    27301 tcaactattt gaacgagcga tccctttgat caaatacatt cctgaaacta gcgtagttga
    27361 tctaaaaggg gtactccaag aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa
    27421 agaaagtcaa aatggcgaac aattctatca aaagaacaat ggctggaaag agatccgcag
    27481 ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg
    27541 taaaaacttg aatcatttaa tgaacgccga aaacaaaact gaaactttta tcactggaat
    27601 tcaaaacaag cagctgaaac aagaagttgc aaagctttta gttgagaact ttatgaaaca
    27661 ggtgtttgat gatggttttt tccatgctga tcctcatcca gggaatctgc tattccatgt
    27721 actaacaaaa gaagaacaga cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga
    27781 gttcggttcc tttgcttttc gagcctccac ttctgcagaa gaccctgttg cgccatatac
    27841 catcaactac atcgattttg gtatgatggg gcatctttct gcaggactta ggcaaaaact
    27901 cacacaagcc gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct
    27961 tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag aactgacaag
    28021 ttttcttgaa cagtattatg attctcctat tgatgaaatc aacttgcaag aagtattcac
    28081 acatgtcgtg acgatttgtc atcaaaataa tctgcagttc gatcgtgata tcacgctttt
    28141 gctgaaagca tttgggacat tagaaggggt gatccgcgtc ctcgatcctg aagtttccct
    28201 catggaagtc gcttcgcctt ttgctcaaca ttattttctc actcatctgg atgtagaaga
    28261 tacattgaaa caaagcggat tagacttatt ggaagggatg aaggctgcgc ctaagatccc
    28321 tcaacagctc caccatctat tagaaatgtg gacttcggga caaggaaaag tcaatttgga
    28381 attgaaaaag caagacaaat tattgagcag gatcgaaagt atgatcaatc ggcttgtttt
    28441 cggtatgatt ttagctgcat tgatcgtagg gtcctccctg cttgtccaag cagcacctgt
    28501 agaaaacgca gaggtcgttt ccttattagg gatatttacg tatgctattg ccgcattcgt
    28561 cattattttt ttggcaatcg atgcattgat ccaaatgtat aagaagagga aaaagtagaa
    28621 aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa ggggttttac ataaattgtt
    28681 tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag gctacttctg aaacaccgag
    28741 aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa aaagggaaca
    28801 tggaacacat ttatcctgtt tccctgttcc ctttttgatg tattcgagca taaataaaat
    28861 accttcgtta cctactatct tctgtacttg gtaaacctta gtcaattgcc ttttttaacg
    28921 cttgacgaaa agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc
    28981 cagcttttct taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt
    29041 cgtcaaattc ttttccagag tgatggaaca ctcgcacacc cttcggcaat acaagtgaag
    29101 ctaaaccata atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat
    29161 aatccgcact atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt
    29221 tggtcgtata atggtccaca ctggtcacga taaccacgtc aagaccatag gattctccta
    29281 attttatgac atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt
    29341 ttcgccttct tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg
    29401 atccattttg ccaagataag cagttgtaca ctttctatcg gcactgattc atggttcagc
    29461 gtgatcaata atagatggat tggctctccc atttcttttg ctaaaacaaa caaaagcgtc
    29521 atcaataaaa actggtggat cacactccct ttattttgag tagtatactt gacaacgatc
    29581 cggcgatcca ttggaatagc tgtatcagtt tgataatatt ccttatttag ccagctgcct
    29641 gttactacgt gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag
    29701 aggaccaata aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgttaaa
    29761 cgtttcaaaa tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa
    29821 tacataaaaa tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaat
    29881 aaaaaaagga aattccctag cattccattt agaaaagtaa acagatttag ccagcttggc
    29941 aacttccatt ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagcagcaag
    30001 aaaccataaa tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa
    30061 agaaacaata agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt
    30121 cttttgatgt ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc
    30181 atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac
    30241 tcaatcgctt gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc
    30301 atattgataa aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc
    30361 catgctgcac tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg
    30421 cctaatattc caagcaatga accagcacca ccaatcagca gagcgatcat cagcaacgtg
    30481 taactatcta gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca
    30541 taaaacagcg taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat
    30601 aaaagataag gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa
    30661 ttacgcccaa atcgtttgag tttttcgaac atgacaatgg ctccttaaac agtcttataa
    30721 ctaatctgct cttcgatttt agcattaaag gggaaataaa ccaattttac agaatatatt
    30781 cttgtaaaaa aaaatacaga aagaagcata agcttctttc tgcattttta cggtacttga
    30841 ttactcaagc agacaagccc ttctccacaa aagtcttgcc cgacacccct gcatccggca
    30901 ggtgataaag atagaaatca attatcctgt gcctccaaaa tcaattagga gctctgtaaa
    30961 ccagagtcct tctcgacccg tcgcataaat ttactttaac acagttccta tgtttatgcg
    31021 agagcttttt tcattttata atcgaccatt tgaaatcctt gaccgatatc tgaaataagt
    31081 tcttctgtaa tctcaaatcc ttcatgctta taaacctcga ttgcttgatg attcccctga
    31141 ttcactcgca aaaagatctc tctttccttt gtttgcgcag caagttcttc ataccagcga
    31201 aaaactgaag aagtcaatcc ttgaccacgt actttattta gcaaatataa tttacttaaa
    31261 taaatttttc catttatctc ttcataagca gtgtaaccga cagtcttttc ttcgcttttt
    31321 aataaaaagt aggacacacc ttctgtcagt tctttttgaa tctgtttttt tgactgataa
    31381 gttttcagca tgtaatccac ttgtttttga ccaatgatcg gcacaaaaac ttccggccat
    31441 acttcttgta tgactgccat cagctcatcc agttcatttt ctgttacttt tattttttcc
    31501 atacacccac gtcctttttt acattataaa tgaccttgcg cttgatggaa taaaaagagc
    31561 atgatcatgc tatacacacc caccgaccac ggtttcaaga aaatcacaat ctgcataaat
    31621 tccctaaaag ataatttcgt caaaccagct actagacaga tcaagtcgtc tggcgcaaag
    31681 ggaagcagca aggtaacaac tagtactttt ttaatatctt tcgtttttct tgtcagcaat
    31741 tcatcaaatt tctgatactt ttttggcgat aaaataagct ggacaaaggc tacgccatag
    31801 taacggacaa gcaaaaatcc gattgcttcc ccaatcacta ggccaataca actatataaa
    31861 agcccaaatc cattgccaaa catcagcatg cccgctactg aggagatccc tcccggcaaa
    31921 ataggaatga tcacttggat cacttgcaac agaacaaata tcatcactgc atacttgcca
    31981 aattgctgga taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg atgataagca
    32041 taaagaatca acacaaagaa aaaaaacaga ccaataatgg gcatgatctg aagacatttt
    32101 tgtaaaactg atcctttttt agacatctat attccccaca tcttttttca tttattaaaa
    32161 ctgcctcct
//
LOCUS       DAPCMT010000017        31311 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPCMT010000017 DAPCMT010000000
VERSION     DAPCMT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.017"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            165..1310
                     /locus_tag="RD743_000649"
     CDS             165..1310
                     /locus_tag="RD743_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDU0562505.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1447..1791
                     /locus_tag="RD743_000650"
     CDS             1447..1791
                     /locus_tag="RD743_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDU0562506.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2062..3348
                     /gene="efmA"
                     /locus_tag="RD743_000651"
     CDS             2062..3348
                     /gene="efmA"
                     /locus_tag="RD743_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDU0562507.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3486..4085)
                     /locus_tag="RD743_000652"
     CDS             complement(3486..4085)
                     /locus_tag="RD743_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDU0562508.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4489..5610
                     /gene="mnmA"
                     /locus_tag="RD743_000653"
     CDS             4489..5610
                     /gene="mnmA"
                     /locus_tag="RD743_000653"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDU0562509.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5856..7277
                     /gene="arcD"
                     /locus_tag="RD743_000654"
     CDS             5856..7277
                     /gene="arcD"
                     /locus_tag="RD743_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDU0562510.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7291..8409
                     /locus_tag="RD743_000655"
     CDS             7291..8409
                     /locus_tag="RD743_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDU0562511.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8469..9761)
                     /locus_tag="RD743_000656"
     CDS             complement(8469..9761)
                     /locus_tag="RD743_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDU0562512.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9893..10471
                     /locus_tag="RD743_000657"
     CDS             9893..10471
                     /locus_tag="RD743_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0562513.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10600..10977
                     /locus_tag="RD743_000658"
     CDS             10600..10977
                     /locus_tag="RD743_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDU0562514.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10910..12982
                     /locus_tag="RD743_000659"
     CDS             10910..12982
                     /locus_tag="RD743_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDU0562515.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13193..14140)
                     /locus_tag="RD743_000660"
     CDS             complement(13193..14140)
                     /locus_tag="RD743_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDU0562516.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14386..14850
                     /locus_tag="RD743_000661"
     CDS             14386..14850
                     /locus_tag="RD743_000661"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDU0562517.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14912..15955)
                     /locus_tag="RD743_000662"
     CDS             complement(14912..15955)
                     /locus_tag="RD743_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDU0562518.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16332..17297)
                     /locus_tag="RD743_000663"
     CDS             complement(16332..17297)
                     /locus_tag="RD743_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDU0562519.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17531..18376
                     /locus_tag="RD743_000664"
     CDS             17531..18376
                     /locus_tag="RD743_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0562520.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18567..19754
                     /locus_tag="RD743_000665"
     CDS             18567..19754
                     /locus_tag="RD743_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDU0562521.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19760..20116
                     /locus_tag="RD743_000666"
     CDS             19760..20116
                     /locus_tag="RD743_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDU0562522.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20118..20456
                     /locus_tag="RD743_000667"
     CDS             20118..20456
                     /locus_tag="RD743_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDU0562523.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    20532..20788
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20851..21885
                     /locus_tag="RD743_000668"
     CDS             20851..21885
                     /locus_tag="RD743_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0562524.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21878..22564
                     /locus_tag="RD743_000669"
     CDS             21878..22564
                     /locus_tag="RD743_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562525.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22588..23436
                     /locus_tag="RD743_000670"
     CDS             22588..23436
                     /locus_tag="RD743_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDU0562526.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23633..24406
                     /gene="sufC"
                     /locus_tag="RD743_000671"
     CDS             23633..24406
                     /gene="sufC"
                     /locus_tag="RD743_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDU0562527.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24419..25705
                     /gene="sufD"
                     /locus_tag="RD743_000672"
     CDS             24419..25705
                     /gene="sufD"
                     /locus_tag="RD743_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDU0562528.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25702..26937
                     /locus_tag="RD743_000673"
     CDS             25702..26937
                     /locus_tag="RD743_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDU0562529.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26924..27394
                     /locus_tag="RD743_000674"
     CDS             26924..27394
                     /locus_tag="RD743_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDU0562530.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27399..28790
                     /gene="sufB"
                     /locus_tag="RD743_000675"
     CDS             27399..28790
                     /gene="sufB"
                     /locus_tag="RD743_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDU0562531.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28885..30027)
                     /locus_tag="RD743_000676"
     CDS             complement(28885..30027)
                     /locus_tag="RD743_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0562532.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30066..30800)
                     /locus_tag="RD743_000677"
     CDS             complement(30066..30800)
                     /locus_tag="RD743_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0562533.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            31032..31307
                     /locus_tag="RD743_000678"
     CDS             31032..31307
                     /locus_tag="RD743_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0562534.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT        10185 a         5733 c         6606 g         8787 t
ORIGIN      
        1 aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt ttgtcacagc
       61 tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc tttggtttgt
      121 tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat gaagtgaaaa
      181 ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt gaagcgatga
      241 cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt ggtcgccacg
      301 cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa gcaaaacctc
      361 acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc ttagaaacag
      421 cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa catcctgctg
      481 ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat ctgcctgtag
      541 atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa gatacgatct
      601 tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc aaagaaatcg
      661 gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa gcgtacggaa
      721 accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct gctcataaaa
      781 tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat atccctgcat
      841 tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat ttagcaggga
      901 tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa gcacgtaaca
      961 acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac gaaatccctt
     1021 atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt cgcttaattg
     1081 gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct atttctactg
     1141 gttcagcttg tacagcagga aatatcgagc cttcacacgt attggaagca atgtatggag
     1201 aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga aataccgaag
     1261 acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa ctagtgaagt
     1321 cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa cttaactgaa
     1381 aaacctagta aaaatcagga taaactttta taataaagag aaactaattt tatcgaggtg
     1441 aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag ctatcgtttg
     1501 catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac aaacggaggc
     1561 aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg attcaaatta
     1621 aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac agcaaatgga
     1681 ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga taaattttat
     1741 tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg atctgtggat
     1801 ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt gtaaggacga
     1861 cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt ttaatatttt
     1921 gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat ttagggaaaa
     1981 aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct atacaaaatg
     2041 aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac gaattggaag
     2101 agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag tatggtcgtc
     2161 caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat tttgagtttt
     2221 gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg accgctagtg
     2281 gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc gattttcgcg
     2341 cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct agtttttgtg
     2401 tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca gtcgattatg
     2461 ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg aatggtccaa
     2521 tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt cgttcctgtc
     2581 aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt attgattttt
     2641 gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat ttttaaagat
     2701 gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat aacgatcaat
     2761 ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt gatgacgcta
     2821 gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta cgcagtcgga
     2881 atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg aatgaaacct
     2941 attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt ggttccaaac
     3001 gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg tgcaacgcca
     3061 tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa tgttttaggt
     3121 cgtgtcttag ggaacttcaa ctctttgatg aacctgacag gtccaattgg attattattt
     3181 gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc cggaatcggg
     3241 attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta tgataaagaa
     3301 ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta tggaatggag
     3361 ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt ggcaataagc
     3421 ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga tatagggcag
     3481 ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat ttgttgctca
     3541 gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg tgcgtctacg
     3601 cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc atgaagattg
     3661 cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata ttgttgatgg
     3721 tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc gtcagtaagc
     3781 ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt ccctttgatc
     3841 aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt tgctttttca
     3901 tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc ttttattact
     3961 gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa gagccgcgga
     4021 gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact gataaagtaa
     4081 ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta tctgagcaac
     4141 tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc gataagaagc
     4201 aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg aaaggctggc
     4261 cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt gattttttgg
     4321 ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta cgacttgata
     4381 attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa aagttgcgac
     4441 cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat gacagacaac
     4501 agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt aacagcactt
     4561 ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg ggatgacacg
     4621 gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa agtagctgct
     4681 caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga tcgtgtattt
     4741 gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt gatgtgcaac
     4801 aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc cgattatgta
     4861 gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca catgcttcgt
     4921 ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca agaacagctt
     4981 tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc aattgctgaa
     5041 cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt tattggtgaa
     5101 aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa tatggtcact
     5161 cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat tggtcaacgt
     5221 caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt tgtaggaaaa
     5281 gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc attgtatgcc
     5341 acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc aaaagaattc
     5401 aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt tcgcttattg
     5461 gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac tcctggacaa
     5521 gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga ccacgcatat
     5581 caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa acagctatgg
     5641 ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca ataaaattaa
     5701 tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc caataatatg
     5761 aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat cttttgcaag
     5821 tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg acgggtatta
     5881 gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt gtgtttggga
     5941 tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca tggataatcg
     6001 ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa aagcgtccag
     6061 atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta ggtggcttca
     6121 tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt gcaacgatgc
     6181 tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat gttccttcta
     6241 ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat ggagttgaag
     6301 gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta ttcctatttt
     6361 taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt tggggtaacg
     6421 tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg cagattaaag
     6481 gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt gttttagcca
     6541 accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg ctttgtttat
     6601 tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa gaagagttag
     6661 caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga aattggggag
     6721 cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg tcttggacga
     6781 tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg aaaggttggg
     6841 gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca atcttgacta
     6901 atctattctt attgactttc ttagttactg attatgccta tcaatttgcc tattctttat
     6961 gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc cagttttctt
     7021 taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt tttgaattga
     7081 tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc tatcttcctg
     7141 gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg ggaaaagaaa
     7201 aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg cttgctgtag
     7261 gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg tgtagaagaa
     7321 tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc gatcgaagca
     7381 ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt tttcgaaaaa
     7441 cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt caaacaagaa
     7501 gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa tggagcgact
     7561 ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt tgtttcgatg
     7621 cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc tgttgacttt
     7681 gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga agccttagtc
     7741 aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg cacattgcta
     7801 gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc atatgtattg
     7861 atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat cgtagatgtc
     7921 tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc aggtattcgt
     7981 attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta tcgagactac
     8041 acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt gaagaataaa
     8101 gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat tttaaaacaa
     8161 tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc tacttctttc
     8221 attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt agaagaaaca
     8281 ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg actaggttac
     8341 tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt cttgaaaaaa
     8401 caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc agtgtttttc
     8461 atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac tcctgaagct
     8521 ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac aagcaagaag
     8581 ttgaccaaaa caagaagaga agtcgaaaga aagacgccac gataaccaaa tgcactggaa
     8641 acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga ttggttataa
     8701 ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc ttggacagca
     8761 gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa ttgccaaaca
     8821 ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc aatcgcaagt
     8881 attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc tatcaaagtg
     8941 gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc atgtccatgc
     9001 agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga tgcttggatg
     9061 atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc tttgaaaatc
     9121 tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg gacgaaaaac
     9181 agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt agggcgatag
     9241 ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc tgtaacagaa
     9301 ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc agttgcgaca
     9361 agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg caatgcgact
     9421 aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat tcctaatgaa
     9481 gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca ccaaggagag
     9541 ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa gttcaattgt
     9601 gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat gactaaacta
     9661 aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg ttcccaagtt
     9721 tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc ttctttctga
     9781 aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca atgaaagtct
     9841 cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt ttatgagagg
     9901 aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact gctttcgaga
     9961 actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca aacgcttgga
    10021 tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca aaacagcgct
    10081 aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg tgggaaaaag
    10141 tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt tttcaaatca
    10201 agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt tattagatgg
    10261 aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat taacgaaaga
    10321 agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc tttccttacg
    10381 ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct atttttggat
    10441 gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta acaattttaa
    10501 gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt gaaaagtgac
    10561 taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt aattaccatg
    10621 ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat tgaagaagaa
    10681 gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg gaacgaaatc
    10741 catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt ttggaatgac
    10801 ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa gggacttgcg
    10861 ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta tgaaacaact
    10921 aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg gtgctaatca
    10981 aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc ttggtcattt
    11041 attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc aaatggacac
    11101 gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag gacatcaatt
    11161 aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca ttgcgacaga
    11221 tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc atttcaataa
    11281 accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa ctggattcaa
    11341 gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg cccgggcaaa
    11401 agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt acgcggataa
    11461 tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc aagaaaagaa
    11521 aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg gaaaagaaca
    11581 agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag cacaaaaact
    11641 tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa aattaaaaac
    11701 ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca atcaacgcta
    11761 tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga cccataagat
    11821 cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa gtacgatgaa
    11881 agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt tgaaaaacgg
    11941 agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc accatgcact
    12001 tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc gaaaaatcta
    12061 tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag tcagccaaac
    12121 aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc gtgtcgtcca
    12181 agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg aaaaaggaat
    12241 gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta aaccattgtc
    12301 agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag ctacgcgtgc
    12361 agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc aaggactgca
    12421 agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag taacaccaga
    12481 tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga aacaagcagg
    12541 aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga tcaaatcaag
    12601 caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat gtggggccaa
    12661 tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt cagattgcaa
    12721 ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat gtcaccggaa
    12781 aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg atggaacgac
    12841 agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg aagaacgacg
    12901 cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag cagcagcact
    12961 aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag tggtcagctt
    13021 caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa tttcggctta
    13081 tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg tgacaagact
    13141 tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta tattattggt
    13201 gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa acaggacgct
    13261 gtttcgtttc taagaagatt ttcccaatat atttcccgat gattccaaga cataacaatt
    13321 ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat gtaggatcac
    13381 caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg acacaagaca
    13441 aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg ataccatcaa
    13501 tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca cgttcgcggt
    13561 tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct tttgaaaatc
    13621 ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc aagcggaaat
    13681 cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg tagaattttt
    13741 tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg gtgccgacac
    13801 aatcgatatc agaagtagtg atccggttga tcatctcagg aagcagttcg ggcggatctt
    13861 gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat ccagcgtaca
    13921 gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa ggataagctg
    13981 aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca ataaacaaat
    14041 attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga ggaacagttt
    14101 tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc ctttcaatcg
    14161 tatgtttata tatggtttac attcaaaatt taataaacct taaataacac ctttaaaatt
    14221 tgattttacg taaaagaaca ggtattcttt ttgtaaacta acgtatttag tttaccatgt
    14281 ttcctcggtt tatcaaactt ttaagaacat tttttattag ttggcaagta gttttggata
    14341 gagaaaaagg aggaagttcc agtctattga ctaggagcaa acaaaatgaa aatacacaca
    14401 cctatcggct ttttatggtt agatgcagat gaatccggat tgactgctgt atcttttcag
    14461 ccaatttcag aaagcgaatc agcgaatcag cgaattttaa cgcgcacaca agaacagttt
    14521 gaagaatatt ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat aaagagaggt
    14581 acgccttttc agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg agaaatccga
    14641 acgtataaag aagtagccgt tgctgttgat agtccgaaag ctgtgcgagc gattgggcag
    14701 gcaaatcgaa ccaatccatt gccaatcgtt attccttgtc atcgagtgat cgggcaaaat
    14761 ggaaaactga caggatatat gggaaacgca atagatgggg ttgaaatcaa acgccaattg
    14821 ttgttgttag aaggatatct gcaaaattaa aaacagttgt gattcggaca ttgttccatg
    14881 cgagaatcac aactgttttt tcatgttcat tctaaacttt gatcagccaa ataaatagta
    14941 aagccatcac tgtttggaga agcccgacag ctaatccata agataagaag cgtgtacctt
    15001 ctttcatgaa ttttgcaaag tctaaacgca gaccaattgc cgctaaagca gtgatctcaa
    15061 accagctgct gataaaatgt gccgattggt caataactac aggtaaagaa aacaagctgt
    15121 tgaccaaaca aaagatcaca aaccctaata cataccaagg aagcggtaat tttcgttttt
    15181 tctgcgtatc agttggcgtt tcaaacagag ctggttgttt cttgtgcatg aatttttcaa
    15241 acagatagac aacaacaacg agcatcataa tacgtagaat cttaaatagc atggcgaatt
    15301 cgacaacaga attgtcaagt aagcttgccc cagcgactac ttgaccaaca gattggagtg
    15361 taccacccaa aagtgcactt ttttcaatga gatttccacc aaacaactgt aatccaagaa
    15421 aagggagagt caacatcatc acagtaccca gtagattgac tagtgtgatg atctgtcctt
    15481 tttcttcctc tttcgcatgg atggaagggg cgattgaagc aatagcagaa gaaccacaaa
    15541 cagcgtttcc tccagccatc atcaaagaag aagtatcgct gaattgcagt tttttaccaa
    15601 tcaagtaagc accgatgata gtgactgaca tttgcagtaa cacaaaaaca attcctttta
    15661 caccaatttg agcaatcgtt tgagctgtga cagttgcacc tagtaaaaaa acagagaatt
    15721 ctaatagttt gctttcagca aattttgtgc cgattgctag aacaggctgt ttaaaaaaag
    15781 tatttcctaa taaaataccc aataaaatag caatagtcgc tccacctaac tgtggcagat
    15841 aaatagcaag aagcctgctg aaggcagcga tggcaaaaga aagcagtaaa ccgggtaaga
    15901 tttcgcggac cgctgaatcc tttacagatt ttattggatg tgacaatgat ttcatttgat
    15961 cggttccttt cgtcaataaa tactaaaagc atactcgaac atagtagcac taaaccagta
    16021 ttttgacaaa agaacaagag gtcgtgacaa aagtcttgta cgacctcttg tttcgaataa
    16081 acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa atttgagaag
    16141 caattttcgt aaattccttc ttatttcttg aagctgacca cttcttttac gacctcttgg
    16201 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccagtaca aaaacatgaa
    16261 cagctgtttt tgtttggccg ttcttattac ttgatgcaga acagcttttt cacaacctat
    16321 tgaatcgatt attaagcgat ttttttagaa acaaattttc catgtttgct atgttcttta
    16381 attaagaaac gtacgtaatg agcaagaacg tagaagacaa ctacacttgc aaacagacat
    16441 tccgcaactt ttaagaaatc caatacgcta ctatagatac ctaaactgtt gaagtaatga
    16501 acagtagtaa atatagctgt tgcagagata acaagcggga atgtaaaggc tgcatagctt
    16561 ggataaaaac gaagcttgaa caaacgtgga agcatagaca gcacaacgaa atacaatact
    16621 tgagataaag cgaacaatgc tacgacaaga taaaaattag gttcagcaaa gtttttaaga
    16681 taacctgtta ggcaaagaga accaggtgct gcaacgatcg taatcagcgg acgtgtcgga
    16741 tgagccatat ttcttacgaa aaagacacgt ttgatgatga tcggcaataa gaacgcatag
    16801 cagatcaatg aaatccaaaa gaaaattttt ccaataagtg ggaagaagtt cccggcagtt
    16861 acagtgatga ccccaaatcc aacgtaaatg ataaaccagc ttggataaat tgcttcaatt
    16921 gaaagatcag ctttgataac atggtgataa ttgaaataaa agacaaggat cacttggaag
    16981 ataacagcgc acagccaaat gaatttgaca attggtgcaa tactttcata tgaaacaaaa
    17041 taagtacaaa taaccatgag tgacatcgta aaagttggtg aaacggaggc gatgattgga
    17101 tcatgcaacg tttgtttcgt gtgttcgaat aagataaaca atttaacaaa aatgaatatc
    17161 ataagaatca tagagatacc gccagttaga ttgcctaaag caacatggtg atagtcttta
    17221 aacagatttc caagggaagc taacccaagg atcaacccgc agatcgggat cggaataatt
    17281 ttcaaaaagt tcttcatata taatattctc ctttttattt aatagatttt agtaccaaga
    17341 tgaacgaata cattgtaaaa tgaataggaa tgtatgatta cgagcaagca gacagctgat
    17401 tgtcttcatc taagttacta gttggatcag caactgtttt gttgtacatt tctattatag
    17461 atggtttttt gtatatttaa atcaaatagt ttttatagta attatcataa aaagttatgg
    17521 aagtggtgcc atgtttaagt tattaacgac ttttcgagca gtatatgaaa caagaaattt
    17581 ttccagagca gcagaagtac tgtttttatc tcaaccagca gtctcgaatc aaatcaaaca
    17641 gttggagcgg gaattgaata tcgatctttt tcaacgtaac ggaagaaaag agatgcttcc
    17701 aactaagcaa gccgacttat tatatgaacg gacactggtt ttgttagaag aatgggaaga
    17761 aactcagcaa aaattgctca atgaacaaaa tgaagaagaa gtatgtcgga tcgttgcttc
    17821 ccacaccttt gctgtctatc ttttgcctca gttgatgaaa catctgatca aaaggtttcc
    17881 taaagtgcat tttgagatcg aaatcacgaa ttctcatgaa tcattagaac aagtagcgaa
    17941 acatgaatcc gattttggtt ttatcgaaaa gccattagag acttccggcg tacaaagata
    18001 ctctttgatg gaagaccagc tagtcatcgc tggggaccca aaaagcaaat tgtggcttgt
    18061 aagagaagca aactctggtg tttttcatta cacgcagcgt tattttgaag agatgaatat
    18121 ccaagataag aagatgatcg tgaaaagtaa cgatgtaatt gttgcgttgc tgaaggaagg
    18181 aatcggtcgc tcgattttgt ctaagcgctc tgttccagaa aatgtgccaa tgactccgtt
    18241 gaatttccat cgatattttt atttcatcca acgaaccaat ctggtttctg atcagctgct
    18301 agagattgcc gaagagatcc ttcgatacaa tgaaagcaca gaaacttctc acagcgagga
    18361 gaatctcagc tggtagataa ttgcatgtat caaaaaaagt tctatgatac actaaagata
    18421 agcagtaggc ttttgtactg catttgctca agcaactgca caaacggaaa gttaattttt
    18481 cccaaatgtc tatggaaaga ctttccatta tctaactgga ccgctataat aatcagcagt
    18541 atggttttta agaaaaggga tgaaacatgg aaaaaaaatt aaaagcttcg aacgataatc
    18601 gtattgatta tggcgtgatt ttaccagtct ttctgttatg tttgattggt ctgctctcat
    18661 tatatgtagc acttagccat gatccgaaca atccagcagt cttgaaaggc gtagggatgc
    18721 aagttgtctg gtatttagtt ggagcggcag caatcgttgt catcatgcat ttgaattcca
    18781 aatggatctg gaaactgact ccttaccttt atggtttggg gttagtggta atgggactct
    18841 tgctcaaatt ctatgatcag aatttagctg cttcaacagg gtctaaaaac tggtttcgat
    18901 tcggtgcgtt tactttccag ccagcagagt tgatgaagat tgcgtacatc ttgatgatgg
    18961 cactgatcgt aactaaacat aatacgcaag taaaagaacg gacgatgaaa tcggactttt
    19021 ggctgattgg gaagctcttg atcgtgacaa taccggtttt agcattgatc atggcccaag
    19081 atgactttgg aacgatgctt gtctttcttg cgatctttgg cgggattttc ctgatgtctg
    19141 gaatctcttg gcgcatcatc gcacctgtag taatcttggc agtagtcgtt ggagcaggaa
    19201 cgatcttctt ggttacgact gaaggcggaa gagacttgct ttataaagtt ggattcaaat
    19261 cttatcagtt tgcacgtatt gattcatggc tggacccttt ccatgatacg tcaggaatga
    19321 gttatcagcc ggcccaagga cttttggcaa ttggtaccgg tggcttgttt ggtaaaggat
    19381 tcaacgtaag caatatctat gtgcctgttc gtgagtccga tatgattttt accgttatcg
    19441 gtgaaaactt cggctttatc ggtggtgcat tcgtcatttt cttgtatttt attttaatct
    19501 atcgtatgat tcgggtgtgt ttcgatacga ataatgaatt ttacgcctat atcgcaagcg
    19561 gtttgatcat gatgctgctg ttccatgtct ttgagaatat tggagcaaat atcggattac
    19621 ttcctttaac agggatccca ttgcctttca ttagtcaggg aggatcctcg attttaggga
    19681 acatgatcgg tatcgggttg atattatcga tgagatacca aaatgaagca cctgcaagat
    19741 cttcaaggag gtaagaaaat tgtacacctt ttactggtac ccgaaatgtt caacatgcaa
    19801 aaaagccaaa gcgtggttag accagcatgg cgttagttat caaaccgttg atatgatcaa
    19861 agaagtaccg aaggcctcaa cgattgagac ttggctagaa acgagtcaag taccaatccg
    19921 tcgcctgttc aacacaagcg ggatgaaata tcgggaactt ggactgaaag atcaagtagg
    19981 cgagtttacg gcttcaaaag cgagccaagt cttatcgaca gatggtatgc tgatcaagcg
    20041 accactgatc ttacgagagg accagcttgt tgcaattgga ttcaatgaaa aaatttatga
    20101 aggagaactg atataaaatg gcaggcaagc atttgaagaa aaaggacaac ctttggatct
    20161 tatttaatgg aaaagaatac tgtgttggtt tgacaaatga agcgcaagaa gaacttggag
    20221 acatcacgtt tgccaatctg ccaaaagcag gacaatcgct aaaaaaagat gaacctctga
    20281 tcgaagttga agcggaaaaa gcggtcaatg aatttgccag cccgctaacg ggtacagttt
    20341 cttctgttaa tgataaaatc ggtgaagata tcaatatctt gaatgattca gatgaaatga
    20401 atgcgtggat cttaagtttc aaagatgttg atccaaaaga atttgacgaa ttataaaatc
    20461 agaaaatcac ttgaaaaaag ttgacactga ttcttccagt tgctataatc ataagcaatc
    20521 ctatatgaaa cgagaaagct ttgaaaagaa gagtacatgt cacggacgtt tgaagagagc
    20581 cctgtttgct gaaaatgggt aacgaaaatg agatggaaga tggtcttaga gcagagtaag
    20641 cgagcgtaag caaacttact acgggagtgc ccgttacagc gcgacagtat acggaaagaa
    20701 gctaagtcca agtactggaa gaggctgtta ttgcgaagta acggtaaatc aaggtggtaa
    20761 cacggaagtt caagctttcg tccttttgct taaggcaaga gggcgaaggc ttttttgttt
    20821 gaaaaatcat aagagaaaga tgtgaatggc atgtcattaa tccaactaac gaatgtcaaa
    20881 aagacattca atgggaaaaa cggaaacatc caagccgtca atgacgtttc actaaacgtt
    20941 gaaaaagggg atatttatgg aatcgtcggc tattctggcg caggaaaaag tacattagtc
    21001 cgtctcctga atggacttga gcttcccacg agcggcgaag tgatcgtaaa cgatcaagat
    21061 attacaaaat taaaaaacaa agaattacgt acattccgaa agaaaatcgg tatgatcttc
    21121 cagcatttca acctgttatg gtcaagaact gtacttgaaa atatccagct gccgctggaa
    21181 ttagctggcg taccaaagag caaacgtaaa gaacgggcag aagaactgtt gcgtttagtc
    21241 gggttagaag gacgcggaca agcatatcct tcacaacttt ccggcggaca gaaacaacgc
    21301 gtcggtatcg cacgagcatt ggcaaatgat cctgagatcc tgctttgtga tgaggctact
    21361 agcgcattgg acccgcaaac aacagaagaa gtattagact tattgttagc gatcaataag
    21421 aaattgaatt tgaccatcgt cttgatcaca catgaaatga atgtgatccg caaaatctgc
    21481 aacaaagtcg ctgtgatgga gttggggaaa gtcgtagaag aaggagacgt attgactgtc
    21541 ttcagacaac cgaaaaaaga agtaaccaaa cgctttgtac aacaagacat cgaaccagaa
    21601 gctgattcga cagaactatt agctgaactc atcaaagaaa atccaagcgg ccgtttggca
    21661 actttgacct ttagcgaaag aaatgccaac gagccagtca tttcccaagc gattcgacgc
    21721 tataacatcg atgtcaacgt tgtgtatgga aagatcaaac aagtgaaaga aggttccttt
    21781 ggttcattga ctgtcttgat gactggtgaa accaaagaat tagatcaagc agaagtgttc
    21841 atcaaagaac aaggagtagg aattgaggtg atccatcgtg gataaaagct tttcagaaac
    21901 atatctagat tttaaacaaa tcaacccgga aacgatgaat caggctatcc ttgatacact
    21961 atatatgaca ttaatctcaa tggtcttagt gacgatcatc ggattagttt tgggattgat
    22021 cctttactct attggacgca aaaacaaacc gtatgctcga atcatttatg gaatcgtatc
    22081 gatcatcagt aatattttcc ggtcaacgcc atttatgatc ctgatggttt tgatcattcc
    22141 atttactaaa gcaatcgtag gcactatgct aggtgcgcaa gcagccattc cagctcttgt
    22201 tttatccgca gcaccatttt atgcccgtct agtagagatt gcttttcgtg aagtcagccc
    22261 tggtgtactg gaagcagcag atgcaatggg agcaagttac tgggaaatca tctggaaggt
    22321 cttgatacct gaaagtattc ccgcattgat ttctggatta acagttacaa cgatctcgat
    22381 gatcggattt actgcgatgg ccggcgcgat cggcgcaggt ggacttggag gtcttgcttg
    22441 gcaggaaggc taccagcgtg gtaatctaac agtgacattc gttgcgactt taattatttt
    22501 actaatcgta tttatcgttc aaggaatcgg tgatttctta actaagaaaa cagacaaaag
    22561 ataataggat atagggggat gtaggatatg aaaaagaaaa tttttggatt tgcagcagcg
    22621 cttttgctga ctgtaggatt agcagcatgc ggtaacaaca gcgacagcaa agattcagca
    22681 aataaagcag acgacacgac attgaaagtc ggcgcttcac cgacaccgca cgcagagatt
    22741 ttggaacatg tgaaaccatt attgaaagat gaaggaatcg atcttgaaat cgtcaaattc
    22801 gatgactatg tattaccgaa ccaatctttg gaagaaggcg acattgatgc caattacttc
    22861 cagcatattc cttatttgaa caaagaaatc aaagaaaaag gatatgactt tgtcaatgcc
    22921 ggtgctgtcc acatcgaacc aatgggcttg tactctaaaa aagtcaaaga tgtttccgaa
    22981 ttaaaagatg gtgcaacaat cattacttca aattctgaat cagactgggg acgcatcatc
    23041 acaattctac aagatgctgg cttagtcaaa gtcaaagatg gagtagatct ggaaacagca
    23101 acattcgatg atattgaaga aaaccctaag aacttgaagt tcaaccatac gatcgaccca
    23161 tcacttttag cttctacgta ccaaaatgat gaaggtgacc tagttgcaat caacgcaaac
    23221 ttcgcttacg gcgcagggtt gaatccatta gaagatgccg tattgcttga aaaggacaac
    23281 tctccttacg tgaatattat tgcgactcgt aaaggtgatg aagacagtga caaaatcaaa
    23341 aaactgattg aagtgcttca ccgcaaagaa gtccaagaat ggatcacaga caaatggggc
    23401 ggctctgtta aaccagtaaa ggctgatgca aaataaggta tggaacaaaa aagatgagaa
    23461 ataagtctgt aatggcttat ttctcatctt ttttatacct attctaattt agaaatcaaa
    23521 attgtttaat aataaataaa agatatttga ttttagtaag gaaggtcatt gcgcttcatt
    23581 ctcaattagt ctaaaatgag tgagaacgaa agagaatttg ggggtataat gcatgtctgt
    23641 cttagaaata aaaaatctac atgtgtcgat cgaggaaaaa gaaatcctta aaggcgtaaa
    23701 cctaacaatg aaaaccggag aaatccatgc catcatggga cctaacggca caggaaaatc
    23761 taccttatct gccgctatca tgggcaatcc aaactatgaa gtaacagaag gcgaaatttt
    23821 gtttgacggg aaaaatgttt tagacttgga agtagatgaa cgtgcgcgac ttggcttatt
    23881 tttagctatg caatatccaa gtgagatacc aggtatcacg aatgcggaat tcatgcgtgc
    23941 agcgatcaat gcgaaacgtg atgaaaacga caaaatttct gttatggcat ttttgaaaaa
    24001 gttagatgaa aaaatggctt tactaaatat gccagaagaa atggctgaac gttatctgaa
    24061 cgaaggtttt tctggcggag aaaagaaacg gaatgagatc ctgcaattgt tgatgttaga
    24121 acctacattt gcaattttag acgaaatcga ttcaggtctg gatatcgatg ctttgaaagt
    24181 cgttgctaaa ggagtaaatg aaatgcgcgg agacaatttc ggtgcattga tcatcaccca
    24241 ctatcaacga ttattgaact atatcacacc tgatgtcgtt cacatcatga tggaaggccg
    24301 agtagtcatg acagggaatg ctgacctagc taaacgtctg gaagcagaag gctacgctgg
    24361 tatcagtaaa gaattaggaa tcgactataa agaagaagaa gcgtaagggg gagcaagaat
    24421 gaaagaaaaa aactggctcg attatcttga cgcggtaaat gatttttctt taagtaaagg
    24481 cgaacctgat tggatgagaa ccttccgtca agacgcacta gcgaaagcag atgaactgcc
    24541 attgccgcat atcgatcggg tgaaattcca tcgctggtcg ttatttgatg tgaaagaaac
    24601 acaaaccatc agtgaaacag gcacgattcc ggcatttgat gcaatgaaag acaatcctgt
    24661 gcttgtccaa caaggttcat ggacgatttt tgaacaactg cctgtggaat tagcagaaaa
    24721 aggagtcatt tttaccgact tgttcactgc aatgatcgaa tatccagaac tagtccaaga
    24781 atattatatg aaaaaagcag taaacatgaa cgaagaccaa ttgactgcac tccatgtagc
    24841 ttttatgaac agcggtattt tcctgtatgt gcctaaaaat gtcgtgatcg atgaaccact
    24901 cgagtcctta ttcattcaag atggcgcatc cgatgagcac ttcttcaagc atgtgttgat
    24961 cgtggcagat gaacatagtg aattttctta tctggaacgt ttccagacaa ccaaagaaca
    25021 agtagcaaaa tcatcaggta atatcatcgt ggaagtcatc gctaaagctg gttcaaaaat
    25081 caaatattca gccgtagatc aattaggaga aaatattact tcttatatga atcgccgtgg
    25141 gcacatttta cgagatgctt ctgtcgactg ggcaattggt gtcatgaatg acggtcatgt
    25201 gatcgctgac tttgactcag atttagcagg agaaggtgca cacgcagaag tcaaaatcgt
    25261 ggcaatcagc tcgggcagac aaatccaagg aatcgataca cgagtaacca ataaagcccc
    25321 tcatacgatc gggcatattt tgcaacacgg agtgatccga gaaaaaggaa cattgacctt
    25381 taatggtatc ggccacatct tgaaaggggc aaaaggtgca gatgcccaac aagaaagtcg
    25441 tgtcttgatg ctttctgata aagcacgtgg tgatgcgaat cctattcttt tgatcgatga
    25501 aaacgaagta actgctggac atgctgccag tgtaggacga gttgatccag aagaaatgta
    25561 ctatctgatg agtagaggat tgcataaaga agaagcagag cgcttagtga ttcgaggatt
    25621 cctcggttct gtcttaacag caatccctgt agaacaagtt cggaaagaat tagtcgaagt
    25681 aatcgaaggg aagttgaatg gatgatcgat gctaaaaagc tacgcaatga ttttcctatc
    25741 ttatttcaag acgtcaatga tgaaccttta gtttatctgg ataatgcagc gacgacccaa
    25801 aagccaaaac aagtactcca ggcattagcg tactattacg aacacgataa cgccaatgtt
    25861 catcgcggtg tccatacgtt agcagaacgc gcgacccatg attatgaaca agcaagagaa
    25921 aaagtacggg cgttcatcca tgcaaaagaa acagcagaag tgctgtttac tcgtggtacg
    25981 acgacaagct tgaattgggt agccagaacc ttcggcgacc gctatatcaa agcaggagac
    26041 gagatagtgc tctcttacat ggaacatcat tctaatgtta tcccgtggca gcagttggca
    26101 aaacgaacag gtgctgtctt gaaatatatc gaactaacag aagaaggctt cctggatatg
    26161 gaggatgcag cgaaaaagat tacggacaaa accaaaatcg tctccattgc tcatgtatcc
    26221 aatgtcttag gtgtcttcaa tcctgttcaa gaattagctg aacttgtcca tgaaaacggc
    26281 ggggtcatcg tagtagatgg tgctcaatct gccccgcaca tggccattga tgtccaagca
    26341 ctagactgtg atttctttgc tttcagcgga cacaaaatgt gcggaccaac aggtattggt
    26401 gttttatatg gtaaacgaaa atggttagaa gaaatggaac ctgttgaatt tggtggcgaa
    26461 atgatcgatt ttgtcgaatt atacgacagt acatggaaag aactaccttg gaaattcgaa
    26521 gcagggacac caaatatcgc cggcgctatc gcattaggcc atgcaatcga ttatttgcaa
    26581 aaactcggta tggacaatat ccatcgatat gaagaagaac tagccgctta tgtgttgcca
    26641 aaattacaag caattgatgg attgactgtt tatggtccac aagacccgaa ttttcggaca
    26701 ggagtcgttg cattcaattt ggatgggtta catccccatg atgtcgcgac agctttagac
    26761 atggaagggg tagccgtcag agcaggtcat cattgtgcgc aaccgctatt gaagtacctt
    26821 gaagtatcag cgactgctcg cgcgagcttt tatttctaca acacaacgca agacgccgac
    26881 cgtttagttg aagcaatcat ggcaacaaag gagtttttcc aacatgggac tatctaaatt
    26941 agacaattta taccgtcaag tgatcttgga tcattcttcc catccgcatc atcgtggaag
    27001 cttgactgac tcgacaagca agatcgaatt gaacaacccc acatgtggag atgtgatcga
    27061 attgcaagtc gatgtaaaag acggaatcat caaaaatatc gcctttgacg gcagtggctg
    27121 ctccatcagt acggcaagtg cctcgatgat gacagacgcc gtcatcggaa aatcaataga
    27181 agaagcagaa catttaactg tagactttct acagcttgtc caaggaaaag aagtcaaaga
    27241 tcccgaccaa ttaggcgatg ctgctatgct tggtggtgta gctaaatttc ccgctcggat
    27301 caaatgtgcg acacttgcct ggaaagcact aggaaaagca atggaaaacg atggtaccgc
    27361 agctttccat cattaccaca aggaagagga gtgagaacat gggcgtacca gaattagaag
    27421 aatataaatt cgggtttcac gatgatgtcg aaccggtttt cagtacagga gaaggcttga
    27481 cagaagaagt gatccgtgag atgtcaagaa tcaaaggcga accagaatgg atgcttgaat
    27541 tccgtctgaa atctctagaa acatttaaca aaatgccaat gcaaacatgg ggccccgacc
    27601 tttcagacat cgatttcgat gccatcaaat attaccaaaa accaagtgac aaacccgctc
    27661 gtgactggga agacgtacca gaaaaaatca aagatacatt tgaaaagatt ggtataccag
    27721 aagctgaacg agcttatcta gccggtgctt ccgcacaata tgaatcagaa gtcgtgtacc
    27781 ataatatgaa agatgaattc gaaaagttag gaatcgtctt tactgacact gactctgctt
    27841 tgaaagaata ccctgaatta ttcaaagagt acttttcaaa acttgtaccg ccaaccgaca
    27901 acaaattagc agcgctgaat tcagccgtat ggtcaggtgg gacctttatt tacgtgccaa
    27961 aaggcgtcaa agtcgatgtt ccattacaaa cgtacttccg gatcaatgcg gaaaacacgg
    28021 ggcaattcga acgtacgctg atcattgttg acgaaggagc aagcatccac tatgtcgaag
    28081 gttgtacggc acctacttat tcaagtaaca gcctgcatgc cgccatcgta gaaatcttta
    28141 caagaaaaga tgcgtattgc cgttacacaa cgatccaaaa ctggtcagac aatgtgtaca
    28201 acttagtaac caaacgagca aaagcatacg aaggcgcaac cgttgaatgg atcgacggaa
    28261 acctaggtgc aaaaacaacg atgaaatacc caagtgttta cttagatgga aaaggtgcac
    28321 gaggcaccat gctctccatc gcctttgcag gagcaaacca aatccaagat acgggtgcaa
    28381 aaatgatcca caatgcacca aatacatcta gctctatcgt ctctaaatca atttcaaaag
    28441 acggcggaga agtaaactat cgaggacaag taacctttgc caaaaacagt gcgggttcga
    28501 tctctcatat cgaatgtgac acgatcatca tggacgaatg gtcgaaatcc gatacgatcc
    28561 cattcaatga gatccacaac agccaagtct ctttagaaca cgaagccaaa gtctcaaaaa
    28621 tctcagaaga acaactctac tatctgatga gccgaggcct gaccgaatct gaagcaactg
    28681 aaatgatcgt catgggcttt gttgaaccat tcaccaaaga acttccaatg gaatatgcag
    28741 tcgaactcaa tcgattgatc agttatgaga tggaaggcag tgttgggtaa acctgttata
    28801 ggagctagaa cccttgatat ataagggttc tagctttttt gttttgcctg tatactagaa
    28861 atgaccagat tttgaccagc tcttttactg atgagccatc atatcagcaa agtcttgacc
    28921 tgttttctta ggtgcattat ttgtaatatg tgcatagatg tctaatgtca ctttactatt
    28981 ttcatgccct aatcgttgtt gaactacctt gacagattct cctgcttcta ataataaact
    29041 agcatgtgta tgtctaaacc catgaggtgt aatacgaggc aagttatatt ttttcaaaat
    29101 atatcttaac caatcattaa cagtgtttgg aatgtataga gtgtttttaa ctgttgtaaa
    29161 aacatgttgc tctttagagc tagtattgta tccaagtttt aaatactctt ctttttgctg
    29221 taatttccag ttgttgagga tagaaagagt ctcagtatct aaagagataa ctcgtctaga
    29281 agctcttgtt ttcggagatt gaattataac tttaccaaat tcatctacag caagagtttt
    29341 attgacatgt agttctttat taaacgtgtc tatgtctttc cactgtaaag cgagtacttc
    29401 actctttctc atacctgtgt atgccaataa tctgaagaag gcgtattgtt tcatgttacc
    29461 aaaatcttta aaagcatcca gtaaattatt aagctcctct tttgagtaaa atttacgatt
    29521 tttctcatac tctttttttc gtggaagaag tgttttttcc ataggattat tgtcaatata
    29581 ttgcattgca accccgtagc gtaaaatttg tgcagtctgt tttctcatat aatcgtaact
    29641 ttcatattta ctgtgccatt catctaccac tttttgacaa tatgggacag agatattcga
    29701 tagtttaagc ttaccaaatg ctggaagtat ttgattattt gcgtatctta ctgagattgc
    29761 gattgttgaa gctttaacac tggtacgctg atgagttatc cattcattat acaggtcttc
    29821 aaatgtcata tcaggtgctt tctttttaac accgtataat atttgatgtc tgtactcgtc
    29881 ataatctttt tgagcttctt gtttgctact aaaacctctt ctcgtagtat ttattttttc
    29941 accagttcgt ggatcggtgg caatgtgtcc tttatacatc cacgccactt gaccgttttt
    30001 ctttttgtac tttgatattt ttgccattaa atcatctcct cttagtttac aaggagtact
    30061 taccactaga tggtatcacc tcccttcatt tttttcttag tgtatttttt cccaattgat
    30121 gaaatcgttt gttctagctt gtttatatta tcagtaattt ctctcacagc ttcttcggga
    30181 taagacatgt ctttcaattg aataggtagc cattcatcta ccattctttc tagtagttct
    30241 tctatactgc tagtgaggaa aagaatgtta ttgtcaaatt cttctacaat agcattgatg
    30301 aataattctt ctatttttaa gatattgaaa agactaattt tttcattttt aactttatta
    30361 caagtcttgt tagcaataaa ttcttgcatc tctggttcta atttggaata tctatccagc
    30421 catgtatcta aattagagtg tactggtggg aactgttgac gaagatgctg gaacaggttt
    30481 ttatctggaa atgttatacc atctagtaca atgctatctc tataaagaat aattcccaaa
    30541 atatattctt ctacattgcc acaaactaac tcctctatac tcatatttcc caaatcagca
    30601 attcgggata atcgctctgg tattggtaaa taggttccct tctcccattc gtagatagtt
    30661 tttttgtcaa ttaattgacc gttgttaatc ataagaccaa atgctggttg tgttaatttt
    30721 ttactctttc ttatagcttt tatttttgtt ccgagtatct tttttcgttc taaataattt
    30781 attggtggac gttttatcat attgaccaca cctcctaaaa ttagtatacc tcaaaaaagg
    30841 ggtgttgcaa ataaatttta caaagctgtt gacacccctt ttttatcgag gtattatgtg
    30901 tatgtcgatg aactggtaat cattttttgt ttgattaatc ggttaataac aaaatacttt
    30961 tattttgagc aaaaaatacg aagataattc ttagaaaatt tgttcaagca atcacgaaag
    31021 gagggggaaa tatgtctcta caaatcattg atagcattgt ttctatacaa gttgaacaac
    31081 aagtgaaaga gataattaca gtgctaaagc ctatgctaga agacattatt tctggagtca
    31141 ctctagagta tatggactat cagcaggcaa gtgaatatct aggtgtatca gtaggaacga
    31201 ttagaaaata tgtttctcaa tacgggttac ctgttataaa aattgatacg gtcattagat
    31261 ttaaaaaatc tgatattgat agtttcatgg aacggtataa aggttgagta a
//
LOCUS       DAPCMT010000018        29432 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPCMT010000018 DAPCMT010000000
VERSION     DAPCMT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.018"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(36..602)
                     /locus_tag="RD743_000679"
     CDS             complement(36..602)
                     /locus_tag="RD743_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDU0562535.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(630..2132)
                     /gene="eat(A)"
                     /locus_tag="RD743_000680"
     CDS             complement(630..2132)
                     /gene="eat(A)"
                     /locus_tag="RD743_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDU0562536.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2390..3511)
                     /locus_tag="RD743_000681"
     CDS             complement(2390..3511)
                     /locus_tag="RD743_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDU0562537.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3637..4578
                     /locus_tag="RD743_000682"
     CDS             3637..4578
                     /locus_tag="RD743_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDU0562538.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4728..5624)
                     /locus_tag="RD743_000683"
     CDS             complement(4728..5624)
                     /locus_tag="RD743_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDU0562539.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5893..7233
                     /locus_tag="RD743_000684"
     CDS             5893..7233
                     /locus_tag="RD743_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0562540.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7451..7894
                     /locus_tag="RD743_000685"
     CDS             7451..7894
                     /locus_tag="RD743_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDU0562541.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8398..9816)
                     /gene="nhaC"
                     /locus_tag="RD743_000686"
     CDS             complement(8398..9816)
                     /gene="nhaC"
                     /locus_tag="RD743_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDU0562542.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10219..11442)
                     /locus_tag="RD743_000687"
     CDS             complement(10219..11442)
                     /locus_tag="RD743_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDU0562543.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11473..12312)
                     /locus_tag="RD743_000688"
     CDS             complement(11473..12312)
                     /locus_tag="RD743_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDU0562544.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12329..13009)
                     /locus_tag="RD743_000689"
     CDS             complement(12329..13009)
                     /locus_tag="RD743_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562545.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13011..14084)
                     /locus_tag="RD743_000690"
     CDS             complement(13011..14084)
                     /locus_tag="RD743_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0562546.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14140..14378)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14421..14654)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14731..15402)
                     /locus_tag="RD743_000691"
     CDS             complement(14731..15402)
                     /locus_tag="RD743_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDU0562547.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15489..18167)
                     /gene="mgtA"
                     /locus_tag="RD743_000692"
     CDS             complement(15489..18167)
                     /gene="mgtA"
                     /locus_tag="RD743_000692"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDU0562548.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            18826..19263
                     /gene="ndk"
                     /locus_tag="RD743_000693"
     CDS             18826..19263
                     /gene="ndk"
                     /locus_tag="RD743_000693"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDU0562549.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19285..20151)
                     /locus_tag="RD743_000694"
     CDS             complement(19285..20151)
                     /locus_tag="RD743_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDU0562550.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20311..22404)
                     /locus_tag="RD743_000695"
     CDS             complement(20311..22404)
                     /locus_tag="RD743_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDU0562551.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23153..23776)
                     /locus_tag="RD743_000696"
     CDS             complement(23153..23776)
                     /locus_tag="RD743_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562552.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(23935..25332)
                     /locus_tag="RD743_000697"
     CDS             complement(23935..25332)
                     /locus_tag="RD743_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0562553.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25364..26335)
                     /locus_tag="RD743_000698"
     CDS             complement(25364..26335)
                     /locus_tag="RD743_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0562554.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26709..27134
                     /locus_tag="RD743_000699"
     CDS             26709..27134
                     /locus_tag="RD743_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562555.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(27622..28323)
                     /locus_tag="RD743_000700"
     CDS             complement(27622..28323)
                     /locus_tag="RD743_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDU0562556.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(28339..28779)
                     /locus_tag="RD743_000701"
     CDS             complement(28339..28779)
                     /locus_tag="RD743_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDU0562557.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(28779..29354)
                     /locus_tag="RD743_000702"
     CDS             complement(28779..29354)
                     /locus_tag="RD743_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDU0562558.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         8450 a         6116 c         5086 g         9780 t
ORIGIN      
        1 ttgaaatatt ttatttaaat aacacaaaaa cataattact cataagaaac caattctgta
       61 ttatctacca tgaatgtaaa gaacccctca ttaggcatcg gatgaaaata agcattgatc
      121 actcttatca aggcattatg gcaaactaac agatagacat tttcttcgtc ttcgatgcat
      181 tctttcaata aaggaacgat ccgtgcatac acatctaaga ctgactcccc atttgggaat
      241 cgtacagcga acgctaaacg tgctttttga aagttctcat ctttgcttgg cagtccatca
      301 taatccccaa aatccatttc taccaatcgt tcatcaacgg tcagcggcaa gctgagacga
      361 tcagcaactg cttgtgctgt atctcttgct cgttgaagag gggaatggat gattttcgtg
      421 attggaacag gcaaatctac taccttttct gctagtttct gtgcctgcat ctgtcctttt
      481 tcagtcagcg gcacatctgc ccgtccgcaa actaatcctt gtgcgttcca agttgtttct
      541 ccgtgtctcg ttacataaag catgtgattc tctccttcgt tggcaaagtt ccccttaacc
      601 ataccttgtt gttcttgaga ttacaagggt tagattctct caagagaaat aatctcagta
      661 gatactttat ccaggaaggt ttgatcatgt tccactagta acatggcagg tttcacgttc
      721 aagatcagtt gttctaattg ttcttgattg aagacatcca aataattcaa tggttcatcc
      781 catgtatata gttcagctgg ctgtgataaa gatttagcca attccacttt tttccgttgg
      841 cccatactca tctgctcgat cttgttatga aaaacatctc tttccatccc aagctttcgg
      901 aggttgttca aaaatgcttg gtagtctact tggtttttct ctgcaaattc cgccaacgtt
      961 cctcgattgt cttcataatt ttggcttgca taactaatgc tcagatgttt tggctggtat
     1021 ttttctccta taaccttgcc gttgaatgcc cccagaagat aatggataat ggatgacttt
     1081 cctgcaccgt taggacctga aatcgccacc cgctgatgag gctcgattgt aaaatgaatt
     1141 ggctcgaata acagattttc ataccctaat tgaagatctt ctacgcttaa aagtcgctta
     1201 tggtgagacg cttggctatt catcgtcaaa gaatcgatat attcgatatc ttttaatagt
     1261 ttttctttct cgctgatctg ggtctccatc cgattcacga ttgctttgga tcgtttcatc
     1321 gtccgtgcag catcagcacc cactgctcct ttattcactc gtctagattc agtatcgatg
     1381 aatccgactt gtttctttgt tttatctcct tctcgggaac gagaccattc ggctttttca
     1441 gctgctgttt tctttagcct actgacttct tttttcaatt tttcattttg agccatttca
     1501 aattcatcac gaagtttttt ctgttcttca tagatagaga aattcccttg ataaagttcc
     1561 agttgacttt tttcgattgc taaaacatgg tccactactt catcgataaa tccccggtca
     1621 tggctgacca cgatgaagcc ttgttttttc tttttcaaat aagccgctac ttgttttcta
     1681 ccagagatat ccaaatgatt cgttggttca tcgattaacg ggaaatgagt gtcatccaca
     1741 aataaaagtg ccagtaggac ttttgtcttc tctccccccg atagtgtgct gaattctctc
     1801 cataagattt caggatctgt ttgcatcaat tggagctctc tttcgatttc ccatatctca
     1861 aaatccgtaa tatcatttaa cacgtaatag gttaaacgtt ctttatcttt tgtctgttgc
     1921 gggaaatagg caaattcttg ctgatggatt acttgccctt gataaggtag tttgttttgt
     1981 agaatattca gtaaagttgt ctttcctcga ccgtttcgtc cgataagtcc tagtttccat
     2041 tgtgtgtcaa aatttagatt tgcttgttca aataataatg tgccttggct gtcgtagccg
     2101 aatgtcagat tttttatttc gattttagac attttccatc acctcgtaaa aaaagaccag
     2161 tcctattttc agaactggct aaagaggatg caagggttgg atacacacaa aaagacccat
     2221 acatcgaaaa aaccaagcca atcccgaaaa tctgcacact gcttccttgt ctccacaagg
     2281 tacagtctga atgcagcttt catcaatcga cttaattttt caatgcacgg atccctcact
     2341 ttgtcctata tttgaaatta cgatagcata aagaaaatga ttttgtcaac tacttcggga
     2401 ggatggtgaa aaatctgcta agtgtctgtc ctaagatttt tgttttttct aatgtttgcc
     2461 tgtgttccat tgcttcttct aaacctattg gacccaaatg aatacttaag acaattggga
     2521 gaaaatcaga aagtccttct agctcttggt ctctacttcc gcaaatagcg attattggtt
     2581 ttcgatattt ttttgcaagc actgctacac cgtatggaac ttttccgtgg atcgtttgct
     2641 gatcgatccg cccttctcct gtaatgatga catctgcttg ttcgatcttt ttttccaatc
     2701 cagtgatttg gctgactaaa tcgaaaccag aaaccatctt cgctcccaac aaaaaaaggg
     2761 ctccaccaat accgcctgct gctccacttc ctggaatatc atctaaatcc acctcccaga
     2821 aatcttttat ttgctgagca atctttcctg cttgtttatc taagaaagct agcgtcgtat
     2881 ctattcctcc tttttgtctt ccaaattgcc aagcagctcc ttgttgacct gtatatggat
     2941 tggtaacatc tgcagcgata atcaatttta ctttagaaac aagaacgttt cttcgatcaa
     3001 aatgaagttt tttagtagat aataaaggat ttccttctgt gtacccttct aaagaagcac
     3061 ccaaggattg gagtaatcct agaccaccat cgctacaccc gcttcctcct aaagtgacaa
     3121 taatttgttc tacttcttga agcagtgcat ctttaataag ttctcctaag gcataactgg
     3181 aagcaagtcg gatagtttga tccgttggtt tatcaagtag atctaacccc aatgcttgtg
     3241 cagattcgat gactgcggtg ttttttcctt gccaagttgt caataaatag ctgatttttc
     3301 gctgacggaa taagagatcc atcccatcaa tggtacgcca tgttcccctg agcgcgtaat
     3361 agattgcttc tgcagtattt tctccgccat ctgcaatcgg cacattgatt tttcttcctt
     3421 tacgatcttc ccagccattt agaactgcct gatttagttc aaatgaagaa gcacttcctt
     3481 taaatgaatc aattgccgcc aaaatatcca tctgtatccc tcactttctt cttaagtaag
     3541 acactttttt tcattttttt caaacaaaac aatgctattt tcttccattt ttctatatac
     3601 taaaagggaa ctcattgaat aaaggagaga aaaaacatga aaatcgcaat agctggggcc
     3661 ggagcaatgg gcagccgaat cggattgatg ctacatcaaa gcggaaatga ggtcctcttg
     3721 atcgatcgtt ggcctgctca cattgaagcc attcgtacaa atgggttgat tgctgacttt
     3781 aacggtaaag aagtggtcgc aaaactgcca atctattcac cagaagaaat catcgaaagc
     3841 aacgaacacg tggacttgat cgtagcatta acaaaagcca atcaattaga tgatatgttt
     3901 tgttcgatcc aatccattat tactgacaat acttatgttt tgtgtttact aaatggatta
     3961 ggtcatgaag atgtactgga aaaatatgtg ccaaagaaaa atatcttatt tgggatcacg
     4021 atgtggacag ctggtctagc tggtcccgga aaagtcacat tgttaggtga tggagaaatc
     4081 gaactagaga acttggaacc agaaggcgaa gcatttacaa aaaaagttgt agaagtcttt
     4141 caagaagcaa atttaaatcc gatctatagc cataacgttc gttattccat ttggcgaaaa
     4201 gcttgtgtaa atggaacatt gaatggttta tgcacgattt tggattgtaa tattgctgaa
     4261 ctaggcgcgc aaaaagcggc agagagcatg gttcgaacaa tcgtatctga atttgcttct
     4321 atcgcagcaa aagaaggaat catactagat caagaagaag tgtaccaaca tattgcttct
     4381 acttatgatc ctgacaacat cgggttgcat tacccatcta tgtaccaaga cctgatcaaa
     4441 aaccatcgtt tgactgaaat cgactacatc aatggtgcca tttggcgaaa aggacaaaaa
     4501 tatgatattg ctacaccata ttgtgccttt ttgacccaat tagtccatgc aaaagaagga
     4561 atccttggcg cagaataaaa ggctctctga tgagaatcag ctgaatcagc agctggttct
     4621 ctttttttaa gcccaataaa agatcaaaaa gaaaacacaa ctctcatgat gccataagaa
     4681 ttgtgcttgt ttgtttcagt acataatctc ttcaaaatgt aggtttttta tttgttttca
     4741 acagaggaga ggtcatattc gtattgctca taaatagtgg aatcgttagt gataaatcgt
     4801 tttgcccaat tttctgcatc ataaaattcc aatagctctg ataggattgg cttaaatggt
     4861 gtatgcagaa attccggtaa ttcatctaaa tcacgttctc gaagcaaaaa aatattttca
     4921 ggacgataaa aatgataatg agcaatcgct tgattatccg tcactagttt cttttgaaaa
     4981 tacatggatt caaatgtccg caaagtcact ccttgctgac cctcccgcaa cagttccaga
     5041 atcccgcgcg attcccctgt ttttttcagg tactcttcat aagtaagata tccaggttgt
     5101 tcattccctc gatgtggaag aatgaaatag ttggtcgtaa catctctttt tataaattcc
     5161 tgcttatagc tttctgctcg ttcttttctc ccattattcg ttgcaccaaa aaatagatcc
     5221 gtctttggtt cgctagaaga aaaattcata tgtttcgaat aaaatgaact attatgtttc
     5281 agcccaagtt cttttgcttc caaaaaatca aaatgataaa tctcatcgat tgccggttca
     5341 tcttgtgcct gcttcagcat cgcctcgtgt tctgctttga agtgattcca aaaaaataac
     5401 acaatttttg cattggtaaa tgggcgcacg cgtcgagcca tctccatatc aaatccataa
     5461 tctaaaaaaa cgatcagctg ataatttgcc aactgttgtc tagctgattt aatgaattcc
     5521 gactgtagta aatcttttcc ggaccaaaca tctaaagagc ggatggaagg caaaaaataa
     5581 agtgatttat tttttgcaac tagcagtacg tcatttttgt tcatcttttc cgcctcactt
     5641 tctctgtcac tttttcctat gatagcctgt ttaaatagta aaaagccagt aaaacgtttc
     5701 ctatatttgt atgaaaattt aacatttgta tgaaaatatg atgaaataaa tcattcttct
     5761 attaaaattc gcctaaaagt tttctgaata gttgtaattg tctgaaaatt tgttatcatt
     5821 agtcattgtt aaatattgct atttattctt aatataaatt ttgattgtga atttaaaaga
     5881 aagaggtcat ttatggaaga taaaaaattg aaaaaacaac ttagctccag acatatcacc
     5941 atgttagctt taggtggagc aatcggtgca ggtctattta aaggaagtgg cgaagcgatt
     6001 gggatcgccg gcccttctgt attgattgct tttttgattg gtgggctgat tttatatatc
     6061 gtgatgaaag gtctgggaaa aatcgtttta agcggtggcg acacgcatca cggattatct
     6121 ggtttgatac gcccttatct aggtgctcat tctgctgatt ttactgattg ggtctactgg
     6181 tctatgtgga ttatcaatat catcgctgaa gctgttgcag ctgcttcctt tttgcagttg
     6241 tggttcccgc acattccagc ctgggtattt gtttttatgt tggctgtttt aactacgatc
     6301 atcaatcttt attcggtacg gttattcgca gaaacagaat attggctggc ttttgctaaa
     6361 atcagtgtca ttatcttact gatcattttt gctacttatc ttgtcggtca gcaaatgttg
     6421 ggcacaggcg ttttccctac ccttcagcaa ttgactgacc atggtgggtt tactcctcat
     6481 ggtatgaagg gaatcatcag ttctttgctt gtagttatct attcttacgg cggttctgaa
     6541 ctgattgcta tcacggtaag cgaagcagat gatcctaaac gtgcgattcc taaagcaatc
     6601 aaaggtgtca tgggaagaat catttccttt tacatcattc ctttgttttt gcttttgatt
     6661 ctttttcctt ggaatacttt agcaggcacg aatgtcagtc cttttgtgat ggtatttgaa
     6721 aaaatgaata tcccatttgc agcggatatc gtcaattttg tgattgtcct tgctttgttc
     6781 tcatctatca attcgggtgt gtatgcatct tcaagaattt tgttcttccg tttgaaggat
     6841 cgaaaaggtt cctctagaaa actagctgtt ttaaacaagc atcaagtacc gcaacgtgct
     6901 gttttatttt gtaccagtgt tctttatctt ggtgtagctc tttcttattt tgtcggtgac
     6961 aaattatttg gctatctggc aggttccctt tcgtatacag tattattgat ctggatcatt
     7021 atcagtgcgg catcctttgt actggcacta agaaaaggaa cattatggga taaaggaatc
     7081 agcttttttg cattagctgt cttaggattg attttccttg gtatcctgtt cacgaactcc
     7141 attggggtaa ctgtattgac tggcctgctt tacctgttca tttatttcag ttatcaaaaa
     7201 aagaatgatg cgttcgtatt gacgaatgaa tgaaatcgat agaaaatttt attttattta
     7261 tgtggaatag atggagctat tgaagaaagg agttgtgaca aaagtcttgt tgcaactcct
     7321 ttttccgaat aaacagtctc atgacctctt ggtaaacggt gttcaaaaac cctctgtgca
     7381 aaataattga ttgttctcca tgctcatgac atgatagaag aaaggaatga aaaaaaggag
     7441 tttgttttaa atgaaaaagt attggaagtt tttgttgatg atcggtgttt caacggtagt
     7501 tatgtttggt atgatgtact tgaatgtcta tgcgctcgat catatcttct tcagtcgtac
     7561 acgagtattc atggcgttga tgatgggtgc agtcatggca gtcatcatgc tgttgttcat
     7621 gtggaaaatg tatgaaaaca aaaagataaa tgttatgatc ttagccgcta gtcttcttgt
     7681 ttttgccggt tccttgttta tggtacgaag ccaaactact gtcaacgaca cagcttggat
     7741 gaaggccatg attcctcatc attctatcgc tatcctggca agtaaacgag ctgatttaaa
     7801 agatcctgaa gtaaaaaaat tagcggaaga aatcattcag gcacaagaag aagagatcga
     7861 caaaatgaaa aaaatgatca aagaaatgga atgaccatta tccgtccaaa taaaaaaaga
     7921 ggatgttcca gaagttatca gcttcaagaa ataagaagaa gctacttctg gaacgccgtg
     7981 aataaggggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata
     8041 gcttctccta tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg
     8101 tttattaaga ggttgtaaaa tcagcgttct gacctgagta ttagagagac aaatggaaaa
     8161 atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg attgaacacc
     8221 gtttatcgag aggttgtgaa aaagtcgctt agtttcgcgt attaagaaaa attttgtgaa
     8281 atggctttcc acatttttgt ataaatttgg cttaataccg agaaactggc ttttgaatac
     8341 cgtttattcg taaaagggat tgtgacaaaa gtcttgtcac aatccctttt tatgttttta
     8401 ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat
     8461 tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac
     8521 gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat
     8581 tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac
     8641 agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac
     8701 agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa
     8761 aatcaaattt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa
     8821 gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg
     8881 catcatactt tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt
     8941 tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg
     9001 aataaaaatc attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac
     9061 ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt
     9121 aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact
     9181 tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca
     9241 catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt
     9301 tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac
     9361 aaccagcgct ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt
     9421 tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac
     9481 agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat
     9541 gatccctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg
     9601 gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct
     9661 ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga
     9721 gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt
     9781 tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt
     9841 tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc
     9901 tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac
     9961 cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc
    10021 catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa
    10081 cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat
    10141 ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac
    10201 acacaagttt attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc
    10261 ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt
    10321 attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc
    10381 agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg
    10441 gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg
    10501 ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc
    10561 atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa
    10621 agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt
    10681 atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat
    10741 gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa
    10801 ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac
    10861 tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg
    10921 atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc
    10981 tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc
    11041 gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa
    11101 ttgcttcact atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc
    11161 atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt
    11221 attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca
    11281 aaacctttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact
    11341 tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg
    11401 gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc
    11461 tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga
    11521 tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata
    11581 cgttttattg ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg
    11641 gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc
    11701 tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc
    11761 atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc
    11821 ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct
    11881 gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac
    11941 atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac
    12001 taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt
    12061 caaatcaatc gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac
    12121 gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt
    12181 atcttctcca ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt
    12241 tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat
    12301 aatttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac
    12361 tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat
    12421 ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta
    12481 ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa
    12541 tgataccagg caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc
    12601 ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt
    12661 gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa
    12721 tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag
    12781 ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta
    12841 gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc
    12901 caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat
    12961 ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct
    13021 ccttcgatca ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca
    13081 ccagatttga tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta
    13141 acgatcaaac tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc
    13201 gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca
    13261 atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca
    13321 atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat
    13381 tcttcttcag gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct
    13441 tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg
    13501 acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc
    13561 gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc
    13621 aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca
    13681 gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc
    13741 tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt
    13801 tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct
    13861 ttgggagaga gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc
    13921 tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt
    13981 ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt
    14041 tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat
    14101 ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt
    14161 gttaccacct catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact
    14221 ctagcaagat atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc
    14281 gatggccatt ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa
    14341 aggttcttct atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt
    14401 actaacataa actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag
    14461 tttccttccc tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga
    14521 acagttccga atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc
    14581 ttacttgact cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt
    14641 ttcaacgaaa tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca
    14701 agtacgaaat tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc
    14761 aataacagtt cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac
    14821 ggtataattt cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga
    14881 agcagctgtt gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc
    14941 tgttcgttcc aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc
    15001 tccgaatgta aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc
    15061 tcttttcgta acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc
    15121 caatatttct tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa
    15181 atattcttca cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac
    15241 taaaatatca aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg
    15301 gatatggata tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt
    15361 gtcaactaac aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc
    15421 caaaacaata cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc
    15481 cttctcgttt ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca
    15541 agcgccagat aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc
    15601 atcaagccta attcagcacc aaaacttgta aaaggaagga cagaaccgac aataattcct
    15661 aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt
    15721 gtacgcagtg catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca
    15781 tggaataatg cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc
    15841 actacagcag gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa
    15901 cttgttggac caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc
    15961 tcgtccaaat attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag
    16021 atcagattca aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt
    16081 gcaatcagca cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc
    16141 atgatattcc caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct
    16201 ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa
    16261 ataccgatat cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg
    16321 acagtatgac catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat
    16381 tttacgaata cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag
    16441 attttctcac cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt
    16501 gtaactaatt cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct
    16561 ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa
    16621 accatgtctg actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt
    16681 gcgacaccaa tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct
    16741 tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg
    16801 ttcagcattt cttcaatcgc gccttttgtg atcatttgtg ttttaccagc tggatcagca
    16861 accacaacac tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa
    16921 ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa
    16981 tttttcagcc ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct
    17041 tcatttcctt cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc
    17101 gttttatccg tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg
    17161 atcactgttc cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc
    17221 attggaagca tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct
    17281 agccaatccc cttttgtaag cccattgatg acgataactg ctggagccat caatgacata
    17341 aatttgatca gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt
    17401 ttttcagata gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg
    17461 attccttccg cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt
    17521 gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc
    17581 cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct
    17641 ggtatcatat cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga
    17701 atctctgtgt actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt
    17761 ttttcagccg cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata
    17821 accatagtga agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca
    17881 tattccgtga tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg
    17941 tatgctttca atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca
    18001 ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg
    18061 aatagttcaa ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta
    18121 tctttctttt tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc
    18181 ttttggtctt tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag
    18241 atagataata gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt
    18301 tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag
    18361 ttatttttcc aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca
    18421 aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc
    18481 tcagatctca tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag
    18541 ctaccttatg aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg
    18601 tttttgggca gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt
    18661 ccttcgttaa tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata
    18721 ctttttttaa aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc
    18781 cacctcccgc tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt
    18841 gttattatca agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt
    18901 gaagcaaagg gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca
    18961 aaagaacatt atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg
    19021 acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt
    19081 aagatggttg gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac
    19141 gcgctgccgg ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa
    19201 gagatcgccc gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag
    19261 tgatacaaat tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt
    19321 ctctaaggac ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg
    19381 gtagtctttc gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc
    19441 catcaatgtt tcatagatca ctgtacttct ttctttatgt gctaagctcc gtttcatatc
    19501 ttcattcaca ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt
    19561 cattaaaaaa gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct
    19621 ttttttgatt ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc
    19681 agctagaata gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat
    19741 gattcggcat tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac
    19801 catgtgaaga ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt
    19861 attgctgatc tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct
    19921 ttttttcttt aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag
    19981 tgtcatgttc attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga
    20041 atttgtctca atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg
    20101 tgtcaacttc ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct
    20161 cctttttaat ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca
    20221 cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca accttcaatc attagaatgt
    20281 tgtctaacta ctgaaggttg gtttgattgt ttattgatta tttttttgct gttcaacgtc
    20341 tgttcttgtt actggatata agttggttgt tgttttatta ccattttttg aaaagacact
    20401 tgtcgaatgt tcgccttttt gcttttcgat tgcttcatta cgttccgctt catttttata
    20461 gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc gcaagagatt
    20521 ttcctgattc agcttatctg aaagacttaa cgcagtcttg actttttcct gatctttgga
    20581 gatttcctca tcgattcctg ctgctttcgt atcgatgatt tctcctgtct gattattgta
    20641 agcttttcct cctaggacgg tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac
    20701 tacttgatca tgctgagaag ataataagtc tgtaccaaaa tgaagataat ctttatcatc
    20761 gattcccaat agatgtagta atgtcggcaa aacatcgatt tctccaccgt attcatgatt
    20821 gactgtccct ttagtataac caggcatatg gatcatcaat ggcactcgtt gcatctgtgc
    20881 gttgtcaaat tcatcccatg tatctggatc tttaccaaga gcgcttgcta aatctttatt
    20941 atctgtattc gagatcccaa aatgatcccc gtaaatcaca aagattgaat tttggtaaat
    21001 accagatttt ttcaaatacg taaaaaattg ttccaatgat tgatccagat aatgtgccgt
    21061 tcgaacataa ttatctactg tgctattgcc agtattcaaa gatggaaagt cgaaattctt
    21121 gtcatccgta taataaggcg tgtggttggt tacggaaaga aatttcgtgt aaaacggctg
    21181 ttgtagatgc tcaagatatt gggcagattc tctgaataaa tctttatcta atatcccata
    21241 acctaacgtt tcgtcggatt catcaaaata agatcgatca aagaaattgt cataacctaa
    21301 gtttttatat acatgatctc tattccaaaa actagctaca ttcccatgga aaacagcgct
    21361 tgtatagcct tgcttttgtc caaggatcgc tggtgcggct tgaaagacat tgtctgaccc
    21421 aagttctgtg aataatgagc cttgaggtaa accaaatgtg ccagtttcca acatattttc
    21481 agcatcactt gtctttccct gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc
    21541 ttgattctgg aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt
    21601 aatcaaaaac tgctggaaac tttctaaatg gagaacgatc acatttttcc ctttagcgat
    21661 cccaaatgtt tcgggatttg gctctgcata atgtttcttt gtataatcta aaacttcatc
    21721 aatcccatta ctgcttgcat gcgcgcgaac actgctagtc attcctgttt tgattccgtc
    21781 ataaatagta tacgcatcga ttcctagata tttgacaata tatgaacggt caaatgttcg
    21841 ctgcagcaat tgtggacggt tggcttcgct cagcgctaga tttcctgaaa aaataagccc
    21901 tgaaagacag gtaacagcga tagccatcgg tttgccgaca gggttgcttt tgataggtat
    21961 tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca gccagtagat
    22021 gacatcatgg ggcttcatca aagaaaagga gcttcctgac aatccttgcg acactttcga
    22081 aaagcctaat acagatttga ctgtaatgaa atctgtgaat tcccgataaa aaataatatt
    22141 caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa agaatagcta acgctggctt
    22201 tggcttttta atatacaaag aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat
    22261 ccacaagata aagtactgga tcgtaccttc cacacctaat gaaaaatctg catagtaggc
    22321 aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa acgattgcta aacgattttg
    22381 gcacaatttt ttaagttgtg tcacgtatat cacttctctc tattcacatt ttttcttatt
    22441 ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat cttaagattt ttttattctt
    22501 tctaaagatt attccgtcca cttttcacag cataaaaccg ctgcgatgta agcgattaaa
    22561 ctaatgaaga gataaaagtc ttgtggtgac cgcttcactc cacggtattc aaaagccagt
    22621 ttctcggtat tcgtacaaaa aaagaggctg tgacaaaatt cttgtcgcag ctccttattt
    22681 cttgatgcag gacagctttt tcacaacttc ttccttaaac ggtgttctcc tgccaactcc
    22741 tcggccacaa gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg
    22801 tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac
    22861 cctcggtatt agattatgtc aggacgctaa ttttacaacc tcttggtaaa cggtgttcaa
    22921 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc tattttttga
    22981 aattgctcct tgtggctcag agttaaacgg caggctcaag acctcttgat acacggtgtt
    23041 caatcagctg accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc
    23101 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctgtt tttcattgtc
    23161 cataggattg atattgtcct tgtgatgact cattcgtaat aattgaggct tgattttctt
    23221 cacttcccgt tatcgtttgt ccactgccag aatctgaagg acttgttcct gtcgactcgt
    23281 ttttttgaga agagacagtt gttttcttga cttgttcatt tgtctttttc cattccgcta
    23341 aacgatcggc gaccactttt ttgtctactt tttcagaata agcggtgatc acttttccat
    23401 ttttcaaacg ggctactttg tcgccatcat gagtcaaaag caaaacggtc aaatgcttgt
    23461 ctttaaattg atattgtctg agagaaatcg cactaagtac gtcatcgata ttaataaatt
    23521 gattttcttc ttttcctttt ggcaaataaa tgtccaattc gcctttttcg taattggcag
    23581 ttattttata ctccgcccac caataagttg agaattcttt tttgaaatta tctactgatt
    23641 ctttgcttgc tacgattttt tctgtttctt tctgagcaag atctgctgtc atttttcctg
    23701 aaaatgctgc ggataaaata agcagtaaga aaaaaccgaa taaccaaaag agaagaaacg
    23761 gtcttttttt cctcatgctt tcctccactc ctatttaatt ttgactattc tattatatac
    23821 aaaaaatgaa atagaaagac aaaaaagttg aaaaagatcg taaataattg aacttctttt
    23881 tcacaaaatg gtaagaatgg agaatttcac gcatttgctc tcaactattc tttcttaagc
    23941 ctcaaatccg tttttatctg atacgtcttt gaaccaatga cccgattttt tgattgttct
    24001 ttttccatct tgtgctaagt ctactgaaat aaagccatat cgattcttat atgcattggt
    24061 ccacgaccag ttatccatac atgtccacat atgatagcct aaacaattgg caccttcttg
    24121 aatggcttga tggacatatt tcagatgaga ttggacaaat tcgatccgat aatcatcgtg
    24181 gatcatgcca tcttctttga taaatcgtgc ttcgttttct acacccatac cgttttcaga
    24241 aataaagcag tcgatatttc cataatgatc tcgtacattg atcaaaatat catagattcc
    24301 ttgttcatag atctcccatc cgcgataagg attcatttta cgaccaggca tttcataatt
    24361 gtcgaaataa tcctcaggca ttggaccgtt tttagtatcg attggtgttt cttttgcttt
    24421 tacccggcgt ggctggtaat aattgatacc taaaagatct actgtatttt ctttgataac
    24481 cgtcaaatcc tctgcatgga tcacaggcat gagatcaagt tcttttacta tctcaatcaa
    24541 ttcacttgga aactcgcctt ttacagctgg atcaaggaat gaacgattga agaacgcatc
    24601 tgctatttgc gctgctttaa catcttcagg gtttgattcg tctcttggat agcttggtgt
    24661 cagattcaag atgataccaa taacaccacc ttgctccatt tcatggtaag atttgatggc
    24721 taacgctgaa gccaacgctt catgatagcc aacttggatc gcttttttca tatctgactc
    24781 ttctggataa tgccatcgat ataaataacc tgcttcgact ggaacgattg gttcattatg
    24841 agtaaaccat ttttttactt tcgaaccaaa caattcaaaa caacttttgg catatttttc
    24901 ataggcttca accgtttctc ggttcaacca tccgcctttt tcttgcagat acatcggcat
    24961 atcgaaatga tacagattca caaagggttc gataccgttt tcaatcaact catcaaaata
    25021 acgatgatag aaacgaacgg cttcttcgtt gattgttcca gattcatcag gtatcaaacg
    25081 gctccattgg atagatgttc gaaatgaatt atgcccagtc tgtttcatca actgtacatc
    25141 ctcttgatac ttcgtataaa cttgcgaggt tttttcaggt cctacttgat caaagaactt
    25201 ttgcggttct tttttgtacc aataatcaaa aatgttctct ccttttccat cgcctgaaat
    25261 ccttccttct gtttgtgggc cacttgctgc agaaccccac caaaaatttg ctggaaattg
    25321 atattttttc atcaaagcac ctctttttct tttttagttc gttttagttg attccatgta
    25381 tgatattttc catcacaatc actttttcat tttcttcctc ttcttttttg attcgtttta
    25441 acagctgttc tgctgctcgt tccccgattt tatctgttgg tggcgtgatc gtgattttcg
    25501 gaaggatact cagatcgttt ttatctaaat aatcatagtg actgaacgca atatccttcc
    25561 cgattctgac accttcttga ttgaaacatt cgatggcacc aatcgacata ttgtaattgc
    25621 tgacaaaaat agcagttgcc cctttttcta ataatgcttt tgccccgtaa tagccgcttt
    25681 ttctagaata gtctcctgta aagacctcta attggatcga tggatcattt tggaccgctt
    25741 ctttggctcc tgctaaacgt tctcttgcta cgtagtctgt ttgagtggca gcaataaatc
    25801 cgattttttt atgtccttgt tcaccatgat gagcgatcac ttttgctaca ctttctcgat
    25861 cattcactaa tattgtatca atacctgctg gtccttctgg acaattcaat gcgataactg
    25921 gaatactcat ttttgtgagc atttcaaatc cagaccactc ttcttcggat aagaagacaa
    25981 tcagcccatc gatcacatgc ttcatcaggt attcaatttt ttgttcaatt ttttctggct
    26041 gatcggcaaa cccgcttaat aataagctat acccattttc ttcagctact ctttcgatcc
    26101 ctgagacaac ttccgaccca aaacggctgg aaatcccgtt tatcaaaagt ccaatagaga
    26161 agcttcgatt attcttcaac cctttagcaa tgatgttttc tttatagtcc aacgctgaaa
    26221 tcgcttctgc tatttttttc atgttctttt ccttcagttg ctgaccattc aagtaacggg
    26281 aaactgttcc cactgaaaca ccagccatct tagccacatc acggatcgat gccatacatt
    26341 ctcactcctg ccagttacat ttgatttctc tttcttgttg ttcgattatg atgaccgcat
    26401 tctttgcata gtgttgcttg tcatcaatat ccatataact aaagtcttca gtagagcaac
    26461 ttcctctcac tattaattat acttaaacaa gtatttgaac cggttcattt ttaatatagc
    26521 atttttttaa aagattgcaa cactttttta aaacgctttc tgaaagtggc ggattttttg
    26581 cctttttaaa attttattct aaaatttact ctatttgtgg gtgttttgtg cgtttttctc
    26641 ttccatccgt ttgaagtttt acattccaat ggtataattt ctttatccta tcaaatgggg
    26701 gaagtatttt gaagaaatta atcgtactat ttattttgtc tgcgggactt ttgacattga
    26761 ctggctgtga tttgtttggc ggctctgacc aatcctcgga cgaaacaacc gattcttctg
    26821 tcttaaaagc ggaaaaagca gataccacca acagtgtata tacgctaaaa gtacgtaaag
    26881 atatggattt atccgcggga cagttgacat ttacagataa aaaaatggaa tggacacgca
    26941 cgtatcagac acaaggaact gattcttctg aatcatctga agaaaaaaca gtgctgacag
    27001 atatcaagat cactaccaaa gatgacgaat atattatttc aggaaaagaa aataaaaaag
    27061 aaacaacagt catatttaca aaaatcggta attaccgttt gaaggatgag gatggcaaca
    27121 tctacagtct atagtgccgt ctgttggatc ttcttttgtt tctccccttc ttttctgcta
    27181 tagtaaagaa gcaagatgat tgtaaaggag acgaaatgga tgatcgatag tcctgattat
    27241 tgtacatgta ataacaacaa aaatatccat acagaattta atgaccaagg aagcttgagc
    27301 atatgcgaca actgtcacaa actagtcaaa gattcattta aaacatttga atcttatcct
    27361 gttcaaaatt atgtaaaaag tagaagaaga taaacaaatc caagcaaaac tcactatggc
    27421 tgatttttat gaacttcaaa ctcagcttct tcctttgatc aagcttcatc agtaaaacaa
    27481 acagtggatc atttcagttt acaaatcaaa gagtaaagat ttatttattt caaatatttg
    27541 aatataaaat aaccaaacga tagaaaggct tattctacat cacgtagtaa tttttctttc
    27601 ccgtcgtttg gttttttatt tttagcctaa ttcttgacct tttgcatgct ggttatccgt
    27661 aaaatatttt ccagcaggct ttccttccaa ttcagctaat ttttcttgga tctgacggat
    27721 cacctttttt gcatcttctg gattttctac aaaatcgaat ttatccccat ccacttgtat
    27781 tttaggacat acatcaaaat cttcatacca ttgattatag cgggtgttca acgtttcata
    27841 ataggtatat aacgtctcat cgctttctag ctgttcatag tcacgcccac gttttttgat
    27901 acgttctagc atcgtgtcca aagaaacttt gatatggacc atcaaatcag gccgtttttt
    27961 tggagcaaca tcatctaact cgtttagcat cgtatctaat aagctatcgt actgctgtac
    28021 ttcgatatcc gttacccgac ctaaatcagc gtttagatgg aacaacaggc tatcttcata
    28081 gatggatcgg tctagcacat tgtcatcgtt cactaaagca tttttcattg ctaaaaatcg
    28141 cttgttcaaa aagaagattt gcaataaaaa agcgtactgc tcaggatttg aataaaagag
    28201 cggcaatact tcgttatctt cgacattttc ataaaatggt ttagaatcaa tctcttttgc
    28261 aagcatatcc gtcaaactgc tcttacctgc accaatcgtt cctgaaatca aaatcactga
    28321 catacaaccc ttcctcttct ataagtactc gcctacgtaa gtttcaacag gcaatgtttc
    28381 aaaatcatat tcacgtacag cttggtctga ttttaaatac gtatggagac tttgtgtaat
    28441 catcaagttc gtaggtaccg tctcttcttg tacaatgatc atcggttttt tcaacatagt
    28501 tgccacacct aattcgtaag ccgttcctgg atcgattgtt tgatgctcaa agtcaaggat
    28561 agcaacaaca aattctgcat ttgtcagttc ttccatatct ttttcgtata cttcctgcgc
    28621 ccatccagcg ctgaatagtt cataattgct ttcttggtga tgacgtggag agtagaaact
    28681 tgtgactgtt ttgttttcct ccaacgcttt ttcgatacga cttactcgat cgatttgctc
    28741 ctctgaaaaa aatggtcctg ctaaatatat ttgactcatt attttgcctc cttcaataat
    28801 aactgatttt taatagatac tgctcgtaat tcattgacca gttccaatgg attgttggaa
    28861 aagattactt gatccaacca ttttttatcc ataaaatcct cttttgcaaa agtttctaat
    28921 aattgttgca agccgctgta ataaccgcca atattcaata ttccgattgg cttgtcgggc
    28981 gtcagaccga ttgctatcca agaaagaagt tgtgagaatt cctccaatgt acctagacct
    29041 ccagggaaaa cgaggacagc atctcccttt tcaaataata atacctttct ttcatcgatc
    29101 gtttctgtag gaataaatgt agtaacatct tttccaggca attcagtttc aaaaagccca
    29161 ttcggataga tacctgtgac ttttccgtta ttttccagta ctgttttact aatcactccc
    29221 ataatcccgg agtatgaacc accgtaaacc agttcgattt cttcttgtgc catgtatttt
    29281 cctaacgttt gcgcaataaa tttgtaactt tctttattac caaaacggga accgcaaaaa
    29341 acagtaagtt tcataaaaaa acactcccta actcaaaagt ctatcttcat tctacacgtg
    29401 tttttttaaa ataaaagact agattttgta gt
//
LOCUS       DAPCMT010000019        28377 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPCMT010000019 DAPCMT010000000
VERSION     DAPCMT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.019"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="RD743_000703"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="RD743_000703"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDU0562559.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="RD743_000704"
     CDS             complement(937..1518)
                     /locus_tag="RD743_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0562560.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="RD743_000705"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="RD743_000705"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDU0562561.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="RD743_000706"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="RD743_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDU0562562.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="RD743_000707"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="RD743_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDU0562563.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="RD743_000708"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="RD743_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDU0562564.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="RD743_000709"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="RD743_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDU0562565.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="RD743_000710"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="RD743_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDU0562566.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="RD743_000711"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="RD743_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDU0562567.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="RD743_000712"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="RD743_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDU0562568.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="RD743_000713"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="RD743_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDU0562569.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="RD743_000714"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="RD743_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDU0562570.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="RD743_000715"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="RD743_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDU0562571.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="RD743_000716"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="RD743_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDU0562572.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="RD743_000717"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="RD743_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDU0562573.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="RD743_000718"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="RD743_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDU0562574.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="RD743_000719"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="RD743_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDU0562575.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="RD743_000720"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="RD743_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDU0562576.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="RD743_000721"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="RD743_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDU0562577.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="RD743_000722"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="RD743_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDU0562578.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="RD743_000723"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="RD743_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDU0562579.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="RD743_000724"
     CDS             13972..14157
                     /locus_tag="RD743_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDU0562580.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="RD743_000725"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="RD743_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDU0562581.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="RD743_000726"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="RD743_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDU0562582.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="RD743_000727"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="RD743_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDU0562583.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="RD743_000728"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="RD743_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDU0562584.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="RD743_000729"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="RD743_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDU0562585.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="RD743_000730"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="RD743_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDU0562586.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="RD743_000731"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="RD743_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDU0562587.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="RD743_000732"
     CDS             18232..18879
                     /locus_tag="RD743_000732"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDU0562588.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="RD743_000733"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="RD743_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDU0562589.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="RD743_000734"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="RD743_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDU0562590.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="RD743_000735"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="RD743_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDU0562591.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="RD743_000736"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="RD743_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDU0562592.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="RD743_000737"
     CDS             20317..21255
                     /locus_tag="RD743_000737"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDU0562593.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="RD743_000738"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="RD743_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDU0562594.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="RD743_000739"
     CDS             21935..22774
                     /locus_tag="RD743_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDU0562595.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="RD743_000740"
     CDS             22750..23619
                     /locus_tag="RD743_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDU0562596.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="RD743_000741"
     CDS             23609..24406
                     /locus_tag="RD743_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDU0562597.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="RD743_000742"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="RD743_000742"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDU0562598.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="RD743_000743"
     CDS             complement(25688..28357)
                     /locus_tag="RD743_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDU0562599.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         9210 a         5281 c         6036 g         7850 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaattt
//
LOCUS       DAPCMT010000020        27911 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPCMT010000020 DAPCMT010000000
VERSION     DAPCMT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.020"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(424..1368)
                     /locus_tag="RD743_000744"
     CDS             complement(424..1368)
                     /locus_tag="RD743_000744"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDU0562600.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1664..2608)
                     /locus_tag="RD743_000745"
     CDS             complement(1664..2608)
                     /locus_tag="RD743_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0562601.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2829..4043
                     /locus_tag="RD743_000746"
     CDS             2829..4043
                     /locus_tag="RD743_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0562602.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4065..6080
                     /locus_tag="RD743_000747"
     CDS             4065..6080
                     /locus_tag="RD743_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDU0562603.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6163..7536)
                     /locus_tag="RD743_000748"
     CDS             complement(6163..7536)
                     /locus_tag="RD743_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDU0562604.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7562..7846)
                     /locus_tag="RD743_000749"
     CDS             complement(7562..7846)
                     /locus_tag="RD743_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0562605.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7848..9863)
                     /locus_tag="RD743_000750"
     CDS             complement(7848..9863)
                     /locus_tag="RD743_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDU0562606.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10042..10347)
                     /locus_tag="RD743_000751"
     CDS             complement(10042..10347)
                     /locus_tag="RD743_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDU0562607.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10436..11467)
                     /locus_tag="RD743_000752"
     CDS             complement(10436..11467)
                     /locus_tag="RD743_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0562608.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11674..12549
                     /locus_tag="RD743_000753"
     CDS             11674..12549
                     /locus_tag="RD743_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDU0562609.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12701..13543)
                     /locus_tag="RD743_000754"
     CDS             complement(12701..13543)
                     /locus_tag="RD743_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDU0562610.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13635..15512)
                     /locus_tag="RD743_000755"
     CDS             complement(13635..15512)
                     /locus_tag="RD743_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDU0562611.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15509..16930)
                     /locus_tag="RD743_000756"
     CDS             complement(15509..16930)
                     /locus_tag="RD743_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDU0562612.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16933..20322)
                     /locus_tag="RD743_000757"
     CDS             complement(16933..20322)
                     /locus_tag="RD743_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDU0562613.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20579..21970
                     /locus_tag="RD743_000758"
     CDS             20579..21970
                     /locus_tag="RD743_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0562614.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22043..22690)
                     /locus_tag="RD743_000759"
     CDS             complement(22043..22690)
                     /locus_tag="RD743_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDU0562615.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22698..23384)
                     /locus_tag="RD743_000760"
     CDS             complement(22698..23384)
                     /locus_tag="RD743_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562616.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23390..24463)
                     /locus_tag="RD743_000761"
     CDS             complement(23390..24463)
                     /locus_tag="RD743_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562617.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24685..25359
                     /locus_tag="RD743_000762"
     CDS             24685..25359
                     /locus_tag="RD743_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0562618.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25364..26704
                     /locus_tag="RD743_000763"
     CDS             25364..26704
                     /locus_tag="RD743_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0562619.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26853..27839)
                     /locus_tag="RD743_000764"
     CDS             complement(26853..27839)
                     /locus_tag="RD743_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDU0562620.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8143 a         5270 c         5057 g         9441 t
ORIGIN      
        1 aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa
       61 tctgcttatg caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa
      121 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
      181 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      241 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      301 cggaccgatt aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa
      361 ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt
      421 ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc
      481 tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat
      541 tgatggcaca cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac
      601 aggcaataca gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc
      661 aataccatag taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt
      721 atcagcaatc acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa
      781 cactggttta ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc
      841 acctaagata tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt
      901 acggaaacga gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc
      961 agattcgttc caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat
     1021 accgtcgaat cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt
     1081 tttaactaaa tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac
     1141 gatatcagca tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc
     1201 ccaagccata ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc
     1261 gatcaatact aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc
     1321 aacaaaacct gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct
     1381 ccttttcata actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca
     1441 acatgttaat tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc
     1501 ataacagata aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta
     1561 gaactgaaaa taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc
     1621 taaaacaatt ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc
     1681 attcattggg agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt
     1741 tatatccgat acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca
     1801 acgcaaagtc gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt
     1861 tcaatttatt actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg
     1921 tacaagaagt aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc
     1981 atccatctgc tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata
     2041 gtgatgtata gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca
     2101 gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt
     2161 gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg
     2221 tgcttgccaa atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga
     2281 ttaaaatatc ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca
     2341 ccacgtcact ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc
     2401 tatacatata attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac
     2461 ggtgtttact gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg
     2521 gtgtctgaag atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca
     2581 gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga
     2641 ctccctcctt tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata
     2701 tgcaaagcta tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga
     2761 ttccgctatt gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg
     2821 aaaggaacat gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta
     2881 ttttgacttc tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca
     2941 cgcgttcttt atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt
     3001 ttgttttgat tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg
     3061 gattagtgat tgcatcgatt tgtggtatcc tccccctctt ctcacaaagt tacagtattg
     3121 tcttagcttc aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca
     3181 gcatgattgc tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg
     3241 catttcaagg atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg
     3301 gttggcaata taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt
     3361 tgtttgttcc tacacctgaa acaactgaac ttcaatcgca tggcacagcc ccaacaagac
     3421 aagtaatcaa aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg
     3481 tggtatataa tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa
     3541 acgaaactaa tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag
     3601 tttgttttgg ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac
     3661 tgatgggaat cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg
     3721 gtgcgatcct aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa
     3781 gtatccaagc gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta
     3841 atttaggtac tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga
     3901 gtaaaacact tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta
     3961 gccttctagt agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa
     4021 aagaacaatt gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac
     4081 ataaagatag atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa
     4141 atttccaacg ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc
     4201 atcggaacgt caagtttaag agatattact ttcttgatca accttccatg tttaaagtga
     4261 cgatccaagg agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc
     4321 tgacttgtcc agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac
     4381 cagcgatgtt tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt
     4441 ttattgaaga atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa
     4501 atcaaatcta ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag
     4561 gaatcttatt cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg
     4621 accatatcga atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg
     4681 attattgcta ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt
     4741 ttcgctattt gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg
     4801 aatttttatc tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga
     4861 tctggtcagt atcagaagaa gccttcaagt tatgtagtga tttgtttttt atcgacggta
     4921 tcaaacgaga tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat
     4981 atcttttttt tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg
     5041 aaatcaagca gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg
     5101 aaaaccacta tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc
     5161 aatcattaat gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag
     5221 caaaagattg gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag
     5281 aacaagtctt attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg
     5341 aaacatatgg agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat
     5401 tgaaacaaaa cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct
     5461 atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt
     5521 ttgctaacaa agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca
     5581 cacagattac tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac
     5641 agacgaatat cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg
     5701 cggtaacttt ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt
     5761 atggagatcc ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt
     5821 taggacgcta ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac
     5881 aacctaatct agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga
     5941 tttcgcttga aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag
     6001 tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct
     6061 attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc
     6121 ttcttctgat attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga
     6181 aacaatgttt gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa
     6241 agcgatgatt ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag
     6301 tcctggtact agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa
     6361 tactggaagg ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa
     6421 taccgcccct cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa
     6481 gaaatgtcct actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat
     6541 ggatagtgtt cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata
     6601 tggaaaaacg ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat
     6661 cccttggaat gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac
     6721 aaacccagct gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt
     6781 gcttagttct ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat
     6841 cgacatcaag agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat
     6901 ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga
     6961 agcataaccg atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt
     7021 cataaacggt tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc
     7081 taataagatc atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag
     7141 acaagaaaca taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct
     7201 agcaagtaaa atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta
     7261 ttgttgcaaa gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag
     7321 agctgtttgg aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc
     7381 gatcagtact aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt
     7441 cttcttctga agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact
     7501 gacgattgac acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt
     7561 tttattcttt tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg
     7621 attgcaaagg tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct
     7681 caatgaagaa ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt
     7741 caaactcgtc cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca
     7801 taaaacttgt tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct
     7861 tttcttatga tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt
     7921 tgtttattag aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg
     7981 atagcagcta aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca
     8041 ggttgttgaa gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca
     8101 tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg
     8161 atcatagctt tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca
     8221 aatgtgattg cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt
     8281 aataattcag atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta
     8341 cgatttaata cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc
     8401 agtagcgccg acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct
     8461 ctttgtactt gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca
     8521 tagagagact ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa
     8581 gaagtttcgt caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc
     8641 gggaataatt gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag
     8701 acgatcacgg ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg
     8761 ccaaatagta tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt
     8821 tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt
     8881 tgatcgatca tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc
     8941 aagtgataaa agacattcat caataattca cgaaacgaag caaattctac ggccatcagg
     9001 aattccatgc gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc
     9061 tggacatctc cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt
     9121 tgatgttcgg aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga
     9181 gaagccaaca gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt
     9241 gtcaaactca aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat
     9301 agtgaaatcg ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt
     9361 gcggttaaat acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga
     9421 ttttttgctt ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta
     9481 gtatacgaca gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac
     9541 aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt
     9601 gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct
     9661 tcaaatcgag taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata
     9721 cggatacggg tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt
     9781 gtatttactt cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg
     9841 agataactaa ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta
     9901 gcgcttttaa atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta
     9961 ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt
    10021 ttatgaattg atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc
    10081 aaatttttct ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc
    10141 attaggcttt gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc
    10201 tgtttcacca gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt
    10261 agttcctgct aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt
    10321 gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca
    10381 tcgtacaata agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata
    10441 tttttccttc tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga
    10501 ttgtttcttc atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt
    10561 cgtagacacg gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt
    10621 gttcatctat ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat
    10681 gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga
    10741 acatcccttt ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg
    10801 attttatcga aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc
    10861 gtcctttccc taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag
    10921 aaagaacttg atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa
    10981 attcatgcgt attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta
    11041 aaatatcccc taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga
    11101 accgtctgtt ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact
    11161 cttctgcttc ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt
    11221 cgagtgccat ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt
    11281 ggatctcagg atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg
    11341 ttttccacac tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt
    11401 ttcttgaaaa aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg
    11461 taagcatgag tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa
    11521 agtatttata atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt
    11581 tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg
    11641 tttatcagta agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa
    11701 caagagttgt ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa
    11761 gctgcagtct acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct
    11821 atcattcgtt tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg
    11881 gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct
    11941 ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag
    12001 tcaataaaaa aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta
    12061 ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg
    12121 aaattccaga ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt
    12181 gattcttctt ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac
    12241 agcggtcgca tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct
    12301 accttgctga ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt
    12361 gttatatcgc aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc
    12421 ttcgattacg tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat
    12481 atccgcaact tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg
    12541 aaaacttaag aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt
    12601 tgatatcatc ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac
    12661 ggaacgattc gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt
    12721 tgaacgattg acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca
    12781 atccatacag gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt
    12841 ttgcatgata aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag
    12901 gtgtacaagt taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat
    12961 cagtcggttc taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt
    13021 cgatatagaa agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag
    13081 gcagtcctcg atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg
    13141 ttccttccaa taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg
    13201 gcaaatggac attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg
    13261 ttctatcctt tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac
    13321 cttcttttgc taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt
    13381 cttcattttc tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat
    13441 tattcagcgc gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga
    13501 aaagcatcag cagatctaga agtttccttt tctttggttt catttgtgtt ctccttgtga
    13561 tgactgtttg ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa
    13621 ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc
    13681 agtagaagga ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga
    13741 agtgtacctt tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca
    13801 taagattgtt catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc
    13861 gctttcgttt cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg
    13921 ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt
    13981 gtggatgggt cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat
    14041 tctgctccag caagtgtttg gtctgatgta acgtcaccat ctactttaac aaatcgtttg
    14101 cccccagtaa cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca
    14161 tttgcttggt tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa
    14221 acgaatttca atgtaccgcc aggagttaat gaaggaatat aattcggatc aactgaaacc
    14281 gtgaatccgt ctgtttgctc agtgacagaa taatttactg ggtcgatttc tttatttcca
    14341 tcatataagg cataagcata cgttcctgaa gaatcattat caaatgttaa agcagcgtca
    14401 tgagtatcga taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt atcagcgata
    14461 cctaatggga tattgacaga aatctcatat tgaatttttt caccgattgc gacatctttt
    14521 ccattcaatt gaggtgttgt tttatcaact ttagacgtat catttttgat ggtcttttct
    14581 actggtgttt ggctatttgc caggatctca aaaggcgtga ttgtttgctt ttcaatcatt
    14641 tccgcattat caggagcttt tacttcttct aaattatatt ttccaacttc taaatgatta
    14701 acgatcaact ggcctttttc aatagatgcc tcggcaaagt cattgttgcc gatgtcataa
    14761 gaatgaccag taatgaaatg tttggctttg gtttgatcag ttgtccaagt gtacaatcca
    14821 tctgtgacac tttggatgta ttttttgacg cttggtgttc cttcttcttt agaaataata
    14881 aattctgctc catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt
    14941 ccatcattac cgactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg
    15001 tatttataag agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg
    15061 tttgcggcag ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc
    15121 ccattttgtt ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa
    15181 gcaactggtg taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct
    15241 cctttatctc gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg
    15301 gctaatcctt ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga
    15361 tcgggtaaat cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca
    15421 aacacttttt ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct
    15481 aacataacgt ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa
    15541 acaaccagat tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg
    15601 tttcgtttgt ttttggaaga aatggacggc tattttcttt tttattgact actttcaata
    15661 tactgtccgc ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt
    15721 cacttaaaat ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca
    15781 acccggaaat tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt
    15841 tctttgtcca cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa
    15901 taaggaatac tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt
    15961 gcttactccc tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg
    16021 ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag
    16081 aaatggtcag tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa
    16141 atccgtgagg tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa
    16201 tcgttaaact attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact
    16261 tttgatagtc aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac
    16321 caatcgtaaa atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt
    16381 cgttttcttc atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat
    16441 gtacaggaag aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg
    16501 attcaatgaa aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag
    16561 aagcgatacc atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg
    16621 aagacgcacc gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc
    16681 gtagatggta aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc
    16741 gatacgtttg taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc
    16801 cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg
    16861 ctacctgagt aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa
    16921 gttttttcat atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa
    16981 cgcacaacca atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg
    17041 taatggtact tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg
    17101 tgacagaaca gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac
    17161 tatagtaact ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt
    17221 ataagttcca ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg
    17281 cgattctaag tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc
    17341 gttgtcttct tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag
    17401 cgtccataca ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt
    17461 atcgatttgg aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc
    17521 aacgactcga tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt
    17581 tgtttggctg tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc
    17641 tgctccttta agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac
    17701 aggaacttca acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac
    17761 tgtttttcca tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt
    17821 tttgcttaat ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt
    17881 ttgcaatttg acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt
    17941 atcaatagaa tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga
    18001 ggaatttttc gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat
    18061 tgcatctatt tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact
    18121 gaatgcttga ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc
    18181 gctatttgca gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc
    18241 tggtttaatg attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg
    18301 ttgaatctca aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca
    18361 taactttttg atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc
    18421 taattcattg gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa
    18481 atttggatgg aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc
    18541 ttggtctttt cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga
    18601 tggagtgacc gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata
    18661 aatgaatggt tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg
    18721 tacagctttt ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta
    18781 aagatcgcct ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt
    18841 tgagagacca gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc
    18901 tcgttctttg agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct
    18961 ggatagattg ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg
    19021 ccgatcttgc gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac
    19081 ccgctgtact ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt
    19141 atgtccattt ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt
    19201 accaccattt gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga
    19261 acccatctgt actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc
    19321 catattgatt ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc
    19381 aatctttact tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa
    19441 tacgagatca agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc
    19501 aaacaatccg ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt
    19561 accggttcct ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga
    19621 atttatacca tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt
    19681 atttcctgta ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc
    19741 tgtaggaatc gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt
    19801 aaatgggctg acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt
    19861 tggtactgac acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt
    19921 attatcgacg atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact
    19981 cagatcgata gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac
    20041 ctgataagca tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag
    20101 accaatttct ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag
    20161 tgcttttttt ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg
    20221 atcatctgta gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat
    20281 cagcatgaaa ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct
    20341 ttttttctgt tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt
    20401 tatttatttt aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt
    20461 cataccaatt ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa
    20521 gaaaagggtt gagggagaaa aacatatgat caggttgttg gaaggaaact atagaaagat
    20581 ggaaaaaatc attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc
    20641 caaacgaatt ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg
    20701 ctggggaacg gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt
    20761 gaatgtacca gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg
    20821 tttattaaat gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat
    20881 ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag
    20941 ctatttatta aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct
    21001 ccagttgcgt atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc
    21061 atttccaatc gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa
    21121 tttgtttcag gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca
    21181 tctcatgaga gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca
    21241 gttgataaat atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa
    21301 gtacagcagc tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc
    21361 ggagaaagaa aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt
    21421 ttcatccgct gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat
    21481 ttattttttc tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta
    21541 ctttgcacag cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact
    21601 tcaattttat tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt
    21661 ttggatgctt atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat
    21721 tatcagcgac ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct
    21781 tgctgtgcat aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata
    21841 caatcttctc gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc
    21901 tcatatattg attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt
    21961 cgagttatga ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac
    22021 aaatacacat agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc
    22081 gaaagtcttt taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt
    22141 tcattacgag ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg
    22201 agaaatgatt gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata
    22261 gttctctacg tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc
    22321 aatagatact ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa
    22381 agaaatgttg tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac
    22441 tgcttttgat cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac
    22501 cgcgcgacat ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg
    22561 agcaaaatat ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga
    22621 aaaaagtttc ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct
    22681 tcattgtcat gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc
    22741 attcgataaa cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc
    22801 gctttttgtt tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct
    22861 gtatccaaac tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg
    22921 cgagcaattg ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta
    22981 agtgactgga tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt
    23041 gaagtaactt ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta
    23101 gagctttgga gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga
    23161 agctcagtta gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct
    23221 cctgaaatgg taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat
    23281 tctccttcct ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa
    23341 ccaaatgttt tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta
    23401 ttgctgtaat tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg
    23461 cgacaatgat caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca
    23521 tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta
    23581 ccaccccaac aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc
    23641 cttgtgcttt cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa
    23701 taccgaccac ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat
    23761 tttgggcaga atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac
    23821 ccttcgatgc tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg
    23881 gctgttcttc ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta
    23941 gggacgcata gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg
    24001 tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag
    24061 ccagattttg agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt
    24121 ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc
    24181 ctttgattgc tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca
    24241 gatctcctct tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa
    24301 tctcttgtgc gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta
    24361 gtcctgacaa catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat
    24421 atcgtaattt agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat
    24481 gatagattga tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt
    24541 gtcacttgtg ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct
    24601 taacatgagt cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag
    24661 tgaccaaatc aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta
    24721 ttatccaata atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat
    24781 ggtagtgaag gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg
    24841 atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag
    24901 actccagtat tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa
    24961 cgaggtgcag atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg
    25021 aaagcattat tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat
    25081 atcagttgtg atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa
    25141 ggaaaagagt ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa
    25201 gagcagatat ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa
    25261 gtttatatga gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa
    25321 accttacgta atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac
    25381 acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt
    25441 tcttttggga ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct
    25501 gtcattagag aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca
    25561 gcaaggcgat ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca
    25621 gccattaatg gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa
    25681 tggtgagata ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa
    25741 aatggatacg aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc
    25801 atctgccctc tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc
    25861 ctacatccaa attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac
    25921 gattatgatt ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt
    25981 gtctagtcta agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga
    26041 gaatgcttct catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct
    26101 ttttaccaag cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc
    26161 agagacgagg cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc
    26221 tgatcagcag ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt
    26281 taaaccattt caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc
    26341 aacattagaa gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga
    26401 taatgcatta aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca
    26461 taaagaatgg ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc
    26521 aaggattttt gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata
    26581 cgggcttggc ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt
    26641 atccaattat cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa
    26701 ataatgctta tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg
    26761 cgaataaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg
    26821 accacttctg tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg
    26881 tgtacatcac cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt
    26941 tcaacatctt catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg
    27001 cttacatcta cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg
    27061 tctgttccct gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt
    27121 ttgaccggta cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct
    27181 ccagttgtca catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca
    27241 tcatctagat ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc
    27301 tcgccaggta cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt
    27361 tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca
    27421 gtccccattg tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt
    27481 tctggctgac gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta
    27541 tattgatata aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa
    27601 aaagtatctt ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca
    27661 tctgtttcaa catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca
    27721 gtagtataaa taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact
    27781 gataatgctt cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg
    27841 taaattcctc ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta
    27901 taggagacga t
//
LOCUS       DAPCMT010000021        27861 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPCMT010000021 DAPCMT010000000
VERSION     DAPCMT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.021"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="RD743_000765"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="RD743_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDU0562621.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="RD743_000766"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="RD743_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDU0562622.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="RD743_000767"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="RD743_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDU0562623.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="RD743_000768"
     CDS             1642..2274
                     /locus_tag="RD743_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDU0562624.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="RD743_000769"
     CDS             complement(2276..3181)
                     /locus_tag="RD743_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDU0562625.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="RD743_000770"
     CDS             complement(3266..3925)
                     /locus_tag="RD743_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDU0562626.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="RD743_000771"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="RD743_000771"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDU0562627.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="RD743_000772"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="RD743_000772"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDU0562628.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="RD743_000773"
     CDS             6168..7253
                     /locus_tag="RD743_000773"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDU0562629.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="RD743_000774"
     CDS             7250..8314
                     /locus_tag="RD743_000774"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDU0562630.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="RD743_000775"
     CDS             complement(8563..9366)
                     /locus_tag="RD743_000775"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDU0562631.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="RD743_000776"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="RD743_000776"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDU0562632.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="RD743_000777"
     CDS             complement(10244..11251)
                     /locus_tag="RD743_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDU0562633.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="RD743_000778"
     CDS             complement(11258..11902)
                     /locus_tag="RD743_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDU0562634.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="RD743_000779"
     CDS             complement(12002..12760)
                     /locus_tag="RD743_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDU0562635.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="RD743_000780"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="RD743_000780"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDU0562636.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="RD743_000781"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="RD743_000781"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDU0562637.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="RD743_000782"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="RD743_000782"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDU0562638.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="RD743_000783"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="RD743_000783"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDU0562639.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="RD743_000784"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="RD743_000784"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDU0562640.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="RD743_000785"
     CDS             18158..19027
                     /locus_tag="RD743_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDU0562641.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="RD743_000786"
     CDS             complement(19153..20052)
                     /locus_tag="RD743_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0562642.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="RD743_000787"
     CDS             complement(20217..21041)
                     /locus_tag="RD743_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDU0562643.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="RD743_000788"
     CDS             complement(21052..22347)
                     /locus_tag="RD743_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDU0562644.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="RD743_000789"
     CDS             complement(22363..22842)
                     /locus_tag="RD743_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDU0562645.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="RD743_000790"
     CDS             complement(22848..23876)
                     /locus_tag="RD743_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDU0562646.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="RD743_000791"
     CDS             complement(23893..24999)
                     /locus_tag="RD743_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDU0562647.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="RD743_000792"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="RD743_000792"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDU0562648.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7854 a         6122 c         5035 g         8850 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa a
//
LOCUS       DAPCMT010000022        26481 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPCMT010000022 DAPCMT010000000
VERSION     DAPCMT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.022"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            86..955
                     /locus_tag="RD743_000793"
     CDS             86..955
                     /locus_tag="RD743_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDU0562649.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1120..2391
                     /locus_tag="RD743_000794"
     CDS             1120..2391
                     /locus_tag="RD743_000794"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDU0562650.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2388..3683
                     /gene="rho"
                     /locus_tag="RD743_000795"
     CDS             2388..3683
                     /gene="rho"
                     /locus_tag="RD743_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDU0562651.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3782..4045
                     /locus_tag="RD743_000796"
     CDS             3782..4045
                     /locus_tag="RD743_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDU0562652.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4490..4939
                     /locus_tag="RD743_000797"
     CDS             4490..4939
                     /locus_tag="RD743_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0562653.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4996..6414
                     /locus_tag="RD743_000798"
     CDS             4996..6414
                     /locus_tag="RD743_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0562654.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6414..7274
                     /locus_tag="RD743_000799"
     CDS             6414..7274
                     /locus_tag="RD743_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDU0562655.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7448..8221
                     /locus_tag="RD743_000800"
     CDS             7448..8221
                     /locus_tag="RD743_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDU0562656.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8368..8733
                     /locus_tag="RD743_000801"
     CDS             8368..8733
                     /locus_tag="RD743_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDU0562657.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8881..10089)
                     /locus_tag="RD743_000802"
     CDS             complement(8881..10089)
                     /locus_tag="RD743_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0562658.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10108..10386)
                     /locus_tag="RD743_000803"
     CDS             complement(10108..10386)
                     /locus_tag="RD743_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562659.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10540..11235
                     /locus_tag="RD743_000804"
     CDS             10540..11235
                     /locus_tag="RD743_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDU0562660.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(11677..12474)
                     /locus_tag="RD743_000805"
     CDS             complement(11677..12474)
                     /locus_tag="RD743_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDU0562661.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12471..13655)
                     /locus_tag="RD743_000806"
     CDS             complement(12471..13655)
                     /locus_tag="RD743_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDU0562662.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13680..14927)
                     /locus_tag="RD743_000807"
     CDS             complement(13680..14927)
                     /locus_tag="RD743_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDU0562663.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15142..16887
                     /gene="dnaX"
                     /locus_tag="RD743_000808"
     CDS             15142..16887
                     /gene="dnaX"
                     /locus_tag="RD743_000808"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDU0562664.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16907..17221
                     /locus_tag="RD743_000809"
     CDS             16907..17221
                     /locus_tag="RD743_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDU0562665.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17246..17842
                     /gene="recR"
                     /locus_tag="RD743_000810"
     CDS             17246..17842
                     /gene="recR"
                     /locus_tag="RD743_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDU0562666.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17906..18550
                     /locus_tag="RD743_000811"
     CDS             17906..18550
                     /locus_tag="RD743_000811"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDU0562667.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18565..18894
                     /locus_tag="RD743_000812"
     CDS             18565..18894
                     /locus_tag="RD743_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDU0562668.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18908..19846
                     /gene="holB"
                     /locus_tag="RD743_000813"
     CDS             18908..19846
                     /gene="holB"
                     /locus_tag="RD743_000813"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDU0562669.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19882..20706
                     /locus_tag="RD743_000814"
     CDS             19882..20706
                     /locus_tag="RD743_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDU0562670.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20699..21046
                     /locus_tag="RD743_000815"
     CDS             20699..21046
                     /locus_tag="RD743_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDU0562671.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21115..21987
                     /gene="rsmI"
                     /locus_tag="RD743_000816"
     CDS             21115..21987
                     /gene="rsmI"
                     /locus_tag="RD743_000816"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDU0562672.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22096..23217
                     /gene="dinB"
                     /locus_tag="RD743_000817"
     CDS             22096..23217
                     /gene="dinB"
                     /locus_tag="RD743_000817"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDU0562673.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23271..23873)
                     /gene="nrdG"
                     /locus_tag="RD743_000818"
     CDS             complement(23271..23873)
                     /gene="nrdG"
                     /locus_tag="RD743_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDU0562674.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24188..26377)
                     /gene="nrdD"
                     /locus_tag="RD743_000819"
     CDS             complement(24188..26377)
                     /gene="nrdD"
                     /locus_tag="RD743_000819"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDU0562675.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKNQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT         8483 a         4827 c         5572 g         7599 t
ORIGIN      
        1 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
       61 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
      121 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
      181 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
      241 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
      301 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
      361 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
      421 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
      481 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
      541 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
      601 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
      661 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
      721 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
      781 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
      841 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
      901 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
      961 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     1021 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     1081 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     1141 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     1201 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     1261 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     1321 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     1381 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     1441 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     1501 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     1561 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     1621 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     1681 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     1741 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     1801 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     1861 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     1921 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     1981 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     2041 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     2101 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     2161 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     2221 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     2281 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     2341 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     2401 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     2461 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     2521 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     2581 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     2641 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     2701 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     2761 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     2821 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     2881 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     2941 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     3001 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     3061 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     3121 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     3181 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     3241 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     3301 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     3361 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     3421 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     3481 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     3541 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     3601 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     3661 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     3721 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     3781 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     3841 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     3901 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     3961 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     4021 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     4081 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     4141 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     4201 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     4261 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     4321 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     4381 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     4441 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     4501 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     4561 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     4621 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     4681 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     4741 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     4801 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     4861 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     4921 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     4981 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
     5041 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
     5101 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
     5161 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
     5221 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
     5281 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
     5341 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
     5401 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
     5461 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
     5521 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
     5581 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
     5641 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
     5701 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
     5761 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
     5821 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
     5881 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg
     5941 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
     6001 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
     6061 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
     6121 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
     6181 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
     6241 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
     6301 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
     6361 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
     6421 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
     6481 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
     6541 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
     6601 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
     6661 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
     6721 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
     6781 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
     6841 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
     6901 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
     6961 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
     7021 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
     7081 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
     7141 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
     7201 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
     7261 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
     7321 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
     7381 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
     7441 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
     7501 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
     7561 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
     7621 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
     7681 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
     7741 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
     7801 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
     7861 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
     7921 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
     7981 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
     8041 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
     8101 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
     8161 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
     8221 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
     8281 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
     8341 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
     8401 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
     8461 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
     8521 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
     8581 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
     8641 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
     8701 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
     8761 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
     8821 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
     8881 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
     8941 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
     9001 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
     9061 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
     9121 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
     9181 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
     9241 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
     9301 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
     9361 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
     9421 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
     9481 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
     9541 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
     9601 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
     9661 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
     9721 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
     9781 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
     9841 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
     9901 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
     9961 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    10021 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    10081 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    10141 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    10201 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    10261 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    10321 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    10381 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    10441 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    10501 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    10561 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    10621 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    10681 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    10741 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    10801 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    10861 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    10921 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    10981 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    11041 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    11101 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    11161 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    11221 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    11281 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    11341 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    11401 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    11461 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    11521 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    11581 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    11641 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    11701 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    11761 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    11821 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    11881 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    11941 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    12001 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    12061 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    12121 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    12181 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    12241 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    12301 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    12361 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    12421 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aactttttca tcatatcaag
    12481 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    12541 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    12601 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    12661 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    12721 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    12781 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    12841 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    12901 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    12961 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    13021 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    13081 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    13141 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    13201 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    13261 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    13321 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    13381 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    13441 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    13501 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    13561 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    13621 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    13681 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    13741 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    13801 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    13861 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    13921 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    13981 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    14041 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    14101 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    14161 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    14221 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    14281 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    14341 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    14401 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    14461 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    14521 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    14581 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    14641 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    14701 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    14761 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    14821 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    14881 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    14941 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    15001 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    15061 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    15121 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    15181 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    15241 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    15301 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    15361 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    15421 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    15481 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    15541 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    15601 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    15661 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    15721 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    15781 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    15841 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    15901 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    15961 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    16021 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    16081 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    16141 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    16201 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    16261 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    16321 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    16381 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    16441 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    16501 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    16561 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    16621 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    16681 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    16741 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    16801 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    16861 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    16921 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    16981 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    17041 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    17101 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    17161 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    17221 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    17281 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    17341 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    17401 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    17461 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    17521 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    17581 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    17641 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    17701 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    17761 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    17821 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    17881 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    17941 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    18001 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    18061 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    18121 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    18181 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    18241 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    18301 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    18361 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    18421 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    18481 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    18541 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    18601 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    18661 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    18721 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    18781 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    18841 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    18901 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    18961 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    19021 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    19081 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    19141 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    19201 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    19261 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    19321 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    19381 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    19441 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    19501 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    19561 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    19621 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    19681 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    19741 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    19801 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    19861 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    19921 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    19981 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    20041 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    20101 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    20161 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    20221 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    20281 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    20341 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    20401 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    20461 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    20521 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    20581 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    20641 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    20701 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    20761 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    20821 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    20881 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    20941 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    21001 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    21061 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    21121 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    21181 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    21241 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    21301 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    21361 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    21421 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    21481 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    21541 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    21601 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    21661 aattttttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    21721 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    21781 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    21841 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    21901 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    21961 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    22021 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    22081 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    22141 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    22201 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    22261 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    22321 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    22381 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    22441 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    22501 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    22561 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    22621 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    22681 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    22741 aaagacctat atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    22801 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    22861 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    22921 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    22981 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    23041 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    23101 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    23161 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    23221 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    23281 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    23341 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    23401 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    23461 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggcatagcct
    23521 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    23581 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    23641 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    23701 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    23761 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    23821 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    23881 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    23941 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    24001 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    24061 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    24121 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    24181 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    24241 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    24301 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    24361 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    24421 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    24481 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    24541 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    24601 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    24661 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    24721 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    24781 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    24841 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    24901 gacgtttccc gaaagaacca tacatatata aaattggcgc atttgctgga atggcttctt
    24961 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    25021 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    25081 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    25141 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    25201 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    25261 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    25321 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    25381 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    25441 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    25501 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    25561 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    25621 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    25681 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    25741 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    25801 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    25861 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    25921 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    25981 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    26041 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    26101 gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    26161 ttttgatttc ttggacaacc aagtctgcaa tcatttgatt tttttcataa gtaagtggtg
    26221 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    26281 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    26341 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    26401 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    26461 attcaatgtg ctgtataatt g
//
LOCUS       DAPCMT010000023        26358 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPCMT010000023 DAPCMT010000000
VERSION     DAPCMT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.023"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(232..600)
                     /locus_tag="RD743_000820"
     CDS             complement(232..600)
                     /locus_tag="RD743_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDU0562676.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            750..1232
                     /locus_tag="RD743_000821"
     CDS             750..1232
                     /locus_tag="RD743_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDU0562677.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1269..2279
                     /locus_tag="RD743_000822"
     CDS             1269..2279
                     /locus_tag="RD743_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDU0562678.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2394..3107)
                     /locus_tag="RD743_000823"
     CDS             complement(2394..3107)
                     /locus_tag="RD743_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562679.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3297..3887)
                     /locus_tag="RD743_000824"
     CDS             complement(3297..3887)
                     /locus_tag="RD743_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDU0562680.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4371..4598
                     /locus_tag="RD743_000825"
     CDS             4371..4598
                     /locus_tag="RD743_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDU0562681.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4614..5780
                     /locus_tag="RD743_000826"
     CDS             4614..5780
                     /locus_tag="RD743_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDU0562682.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6014..7057
                     /gene="hrcA"
                     /locus_tag="RD743_000827"
     CDS             6014..7057
                     /gene="hrcA"
                     /locus_tag="RD743_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDU0562683.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7151..7714
                     /gene="grpE"
                     /locus_tag="RD743_000828"
     CDS             7151..7714
                     /gene="grpE"
                     /locus_tag="RD743_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDU0562684.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7765..9594
                     /gene="dnaK"
                     /locus_tag="RD743_000829"
     CDS             7765..9594
                     /gene="dnaK"
                     /locus_tag="RD743_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDU0562685.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9745..10911
                     /gene="dnaJ"
                     /locus_tag="RD743_000830"
     CDS             9745..10911
                     /gene="dnaJ"
                     /locus_tag="RD743_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDU0562686.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10993..11658)
                     /locus_tag="RD743_000831"
     CDS             complement(10993..11658)
                     /locus_tag="RD743_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0562687.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            11634..11807
                     /locus_tag="RD743_000832"
     CDS             11634..11807
                     /locus_tag="RD743_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562688.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11852..12589)
                     /locus_tag="RD743_000833"
     CDS             complement(11852..12589)
                     /locus_tag="RD743_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDU0562689.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12591..14093)
                     /locus_tag="RD743_000834"
     CDS             complement(12591..14093)
                     /locus_tag="RD743_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDU0562690.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14352..16346
                     /gene="uvrB"
                     /locus_tag="RD743_000835"
     CDS             14352..16346
                     /gene="uvrB"
                     /locus_tag="RD743_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDU0562691.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16356..19172
                     /gene="uvrA"
                     /locus_tag="RD743_000836"
     CDS             16356..19172
                     /gene="uvrA"
                     /locus_tag="RD743_000836"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDU0562692.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19435..20700
                     /locus_tag="RD743_000837"
     CDS             19435..20700
                     /locus_tag="RD743_000837"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDU0562693.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20704..21435
                     /locus_tag="RD743_000838"
     CDS             20704..21435
                     /locus_tag="RD743_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDU0562694.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21570..22454
                     /gene="rapZ"
                     /locus_tag="RD743_000839"
     CDS             21570..22454
                     /gene="rapZ"
                     /locus_tag="RD743_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDU0562695.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22451..23449
                     /locus_tag="RD743_000840"
     CDS             22451..23449
                     /locus_tag="RD743_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDU0562696.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23483..24418
                     /gene="whiA"
                     /locus_tag="RD743_000841"
     CDS             23483..24418
                     /gene="whiA"
                     /locus_tag="RD743_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDU0562697.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24961..25611
                     /locus_tag="RD743_000842"
     CDS             24961..25611
                     /locus_tag="RD743_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562698.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25798..>26358
                     /locus_tag="RD743_000843"
     CDS             25798..>26358
                     /locus_tag="RD743_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDU0562699.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8708 a         4447 c         5600 g         7603 t
ORIGIN      
        1 attcaaaagt tttgcaggaa aattgctgaa aaaataattt tactatgtag aaaattcaaa
       61 gccattcaat cagtgatttt tttcagcaca agaaagactc cttataaaaa tagcagatac
      121 tttgtcaatt aagtgtttaa ccagggatga taaaaaggat aggattgaac aaaacctgta
      181 taaggtgatg aatagtttcc tcgccagtaa aaggtttatt cgtataaagc tttatcgcca
      241 aaatttatca tgaccttttt tatcttcaat attaaatgaa atcaattgtt caatcaattc
      301 ccagttgatt acacttttcc aaggaacttt gatgacgtta tccgtatata cgtagcctgc
      361 ttcttggata tttttttcaa gggcacgaat cgctgctttt tccggtgcga tagaaaaatg
      421 atttttagca aaactaaatg caataataaa ggtaccatgg tcagtaaaca taggttggtt
      481 ccattttaca gttgtgtcta gctggggata cgtttctgtt atcttagcaa aaaggtcttt
      541 gatcttttgt tgatgttctt tttctttgat ttgattgatg atcggctgaa atttttccat
      601 tctaaaacgc ctccattaag tggtttgatt tattttacca gaaaaacaat agttatgtat
      661 aaaaaacgaa caaaacacaa gtaatgttgt aaccgctata tttatatgat aagatgtctc
      721 aaataataaa atagaaaaga gacataaaaa tgattgactt ggcaaagaaa ttcttacacg
      781 aaaatatata ttttgagttg tatcatcatc gagccattta tacaaatgaa gatgctttgg
      841 taataaagaa agaacaagga tttacaggta cagagaccaa aagcttattc ttaaaagata
      901 aaaaagataa taactacgta tatctgactt ttactacgaa acaaacggat ttcaaagcat
      961 tagggaagat cgtcggtaaa aggctttcta ttgtttcatc agaacgcatg gaagaacaaa
     1021 cgggacaaaa gcagggcgct gtttctccgt ttggttatga aaaacagatt ccgattatta
     1081 ttgatgaaga gctcttatca aatgaaaaac ttgtttttgc accaggtaga ccagatcaaa
     1141 cgatggtcat ccaaacggtt gacttaaaca aaatcatcca gctacttgag atgccgatat
     1201 ttttgatgcc ggcaaacgaa aaaaacaatt aaccaagtaa aatagtgcat acttggaggt
     1261 gaagcaacat gataagttat ccattattag aaagaaatgc aacgattggg gttactgcac
     1321 catcctcagg cgtatcagag gaacaccatt cgttactcga atcagcttta aagagaatgg
     1381 aagaaaaatc tttccaagta gttactggaa agacgctttg gacgcaagat aaggaaaaat
     1441 caggtccagc aagaaaacga gcagatgaat tcaatcaaat gatgcaagat gaaaatatcc
     1501 aaatgatctt tccgacttgg ggaggagaat tattgattga gattctcgat tttgtcgatt
     1561 ttgatgctct acaaccaaaa tggatagtag gttattcaga tattagtacg ttgctacttg
     1621 ctgtcacatt aaaaacaggg atagccactg cacatggaac taaccttatt gatttaagag
     1681 gagagtactt ggatgaaacg acagcaatgt gggaaagtgt actgaaaaca agagaaggag
     1741 aatcgattct acagcaatct tctgaaaagt atcaaaaagt gtggccccat gagcattcat
     1801 catcttctgt tttccatctc actgaaccaa caaattggca atcagtcagc ggagatgcag
     1861 taaggatgaa gggacgatta ttaggtggtt gtattgatac gatcaggcat ttaatcggta
     1921 ctccttatgg agatgtccaa tcttttaaaa gaaagtataa tgatggaaag ccgatcttgt
     1981 ggtttctaga aaactgtgag ctatcgtcag ttgatttaag aagatcactt gttcagatga
     2041 aactggcagg ctggtttgat ggcagctcgg ggatcatgtt cggtagaagt gcggcagagg
     2101 atttgttaga ttataaaatg aaggatgtat acgaagatct gtcaaaggaa ttaaaactac
     2161 cgatcttgta tgatattgat tgtggtcatg taccgccaca actgacattg attaatggtg
     2221 cctatgcaga agtagcggta gaagatagaa aagcgactgt tttgcaaacg tttatttaaa
     2281 aaattttttc ctgccttgga gtctatcaaa gaccaataaa tctaaataga aaagaaagtg
     2341 aaagaagctg acaaaatgct gtcagcttct ttttgtataa catattagaa agtctaatta
     2401 tctataactg tcacatcgat taactgtttc acgtcattgg gatttcctgt aggaatagct
     2461 actacagatg cattatcaat gatttcttga gttgtttctc taatgaacgc tggataagat
     2521 gtttgatctg caggcatttg ccaatgtgaa ggaactggat aactagtgat tgtcccgtca
     2581 tggttacttg aatacactaa aactccctgg taactgtatt tcccagtgag cataaccaca
     2641 tctttcggat agactgcact ccctgcatca taaggggcga taggtgttcc tgcaggataa
     2701 tgaataacat gtaattcgaa atcacccgat gtatctctaa aagtattacc tctaatagct
     2761 agccaaacac gtgcgtactc aatttgtaga tcagaatatc ctgctaacgg atctattgac
     2821 tgagaagact gtgaggaggt tgtgtctatt gttgaagaag aaatatttgt ctcaactgta
     2881 ctactagatg tagaagaagt attgggctct gagactttac tgcttgttga agatgtattc
     2941 gtttcagtag aagctgattt ctgagtactt gatttttcag ctgaactggt ttgtctgatt
     3001 gaacttttgt ctgtggtcgc agatggatca ctattttctt tactaggaga cgtacatcct
     3061 acaaggaata aagagaccaa caataaaata aaacctaact ttttcatata aaatacctca
     3121 ttattttttt aataccagta tagcataatc ctgaattttt gaatccgtaa aaccttgtct
     3181 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataatc
     3241 atcagtaaaa ttacttgtca cactattgat aaggagtatt taattgaaga gaatgattag
     3301 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
     3361 gttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
     3421 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
     3481 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
     3541 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
     3601 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
     3661 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
     3721 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
     3781 gaagcagttt tgttttgcca tttctcattt tggtttagtg tgctagtaat ataagcacct
     3841 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
     3901 tttactatct ataacaagga caacagtttt aacctacaga cattcattaa aattgggtag
     3961 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagatagt caacttaatt
     4021 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
     4081 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaata ttaaaagaaa
     4141 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     4201 aaaacaaccc tagagatata taactaggta accagtttaa tgaaagaaaa aatcatccaa
     4261 gaagtcgatt cttggatttt ttagtggatt tgttcgccaa atacgtttag tatgcgtata
     4321 tgagaggttg taagcaaagc gttgtggtct gagcacttaa ggaggaatct gtgaaaatag
     4381 cttttcatat ttttgcagat tttgacttaa tgccgaaggt cagctttgcg attaccgttt
     4441 atcgagaggc tgtaaaagca ccgtgtagct ccgatgacaa taagcaagca tttttagagc
     4501 taggtttcct tgattctaaa tatgcggctt atttcaatga gttggtgctt gaacgcgatt
     4561 ttaagaaggt cgaaaacagg cacgactttt cgacgtaagg agaaagaatt tcaatgatag
     4621 aaacaataaa tcccgcagaa aacaaggttt ctgcgtacat tcatatccca ttttgcgagc
     4681 atatctgcta ttactgtgat tttaataaag tttttttaga agggcagcca gtagatgagt
     4741 atgtggaaat gctgctgaat gaaatgaaaa taatgctaga aagtcatccg acagcgaatt
     4801 tagaaacact ttacgtaggg ggcgggacac caacttcttt atctgctaaa caacttgatc
     4861 gactgttatc aggtgctaga gagatcctgc cttttcaaaa tggtaatgag tttactgtag
     4921 aagcgaatcc aggtgatttg acccgtgaaa aacttcaagt aatgaaaaac tatggcgtca
     4981 atcgtttatc catgggagtg cagacatttg ataatcgctt attgaaaaaa attggacgta
     5041 aacacaccgc agaagatgtg tatcaaacta tgcaattttt agaagcagaa aattttacca
     5101 atgtaagcat cgatttgatt tatgcattac cgggacaaac actagaagga tatctggata
     5161 ctttggaaaa agctttggct ttggatctgc ctcactattc tttgtactca ttgatcctcg
     5221 aaaacaaaac aatgttcatg aactgggtac gacaaggaag actcgaactg cctgatcagg
     5281 aaacggaaac cagaatgttt gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc
     5341 aatatgaaat aagtaacttt gggctctccg gacatgaatc tcaacataac ctcatgtact
     5401 ggaacaatga ccactatttt gggtttggag ctggtgctag cggttatctt ggagatattc
     5461 gttaccgcaa caaaggacca atccaacact atttacgccc attacgagca aatgaacgcc
     5521 cggtattgga agaagaaaaa ttgacacgaa aaaatcaaat agaagaagaa atgttcttag
     5581 gactaagaaa aaaaacaggg attactaaaa aacatttcta tgatcgctat caaaaaacaa
     5641 ttgaatcggt gtacggtgat gtgctaaggg aattagagca agaagggtta cttgttaatg
     5701 aagaagaccg tgtttatttg acaccaagag ggaccttttt ggggaacaat gtgtttgagc
     5761 gctttttgtt tgaaaaatag tttaaagaat atttgttttt tatctgaatt taaataaaaa
     5821 aagttcaaaa atacaataaa ggactattga aacgttgata taactggatt tacatcaatt
     5881 atttagaatt agcactcttg tgtttagagt gctaaaaaat ggagtctttc tcttgacagg
     5941 agaagggctt cttgctatat ttataagtgg attagcactt agaatatatg agtgctaatg
     6001 agaggtgagg aagatgttaa caaagcgaca aaacgacatt ttacgtctga tcatccaaca
     6061 ctatacgagt agtggcgtgc ccgttggttc caaaacattg atgtctgagg gagtcaaggc
     6121 cagctcagca acgattcgca atgatatgaa agcattagaa gatgaaggat tgctgttgaa
     6181 gactcactct tcttctgggc ggattccatc tgctttaggc tatcgttatt acgtggatca
     6241 tttgttaaaa cctgctcgcg tagcagaaga tgatttgctg ctgattcgtc agtctctagg
     6301 cagagaattt catgaaatca atgagattat taagcaatct gctgagatat tatctgagct
     6361 gacaagctat acgacttttt cattgggacc tgaaataaaa gaccgccgac tgactggctt
     6421 tcgtatcgta cccttgaata atcgacagat tattgctatc gtcgtaacag atatggggaa
     6481 tgtagaaagc caagtgttca ctcttcccga atatatggga agtcaggaac tagaaaaaat
     6541 ggtacagatt gtaaacgacc gtctagtcgg agatcctctg atgacggtgt accataaact
     6601 tcgtacggag attccgatga ttcttcataa atatttccaa acgacagaag ggatgtctca
     6661 gttatttgac accgttcttg gtcatgtttt tgaagataag gtttatgtca gtggacaaat
     6721 gaatctcttg gattatgagc cccgtcaaga tcttgatcag ttcaaatcga tgttttcttt
     6781 tatgaaagac tcagacggac ttacacaaat gatcactccg atggatagca atattcatat
     6841 caagattggt gctgaattag gcaatgattt attgcagaat atgagtatga ttcaagcaag
     6901 ttacgagata gatggtcatg gaagaggtac aatagccttg ttagggccta ctagtatgcc
     6961 ttattctcgg atgttcggtc tagtcgatgt ttatcgaaga gaactagcag ctaaacttgc
     7021 tgattactac cgttcgcttg acttgtcaaa ctcgtaatgt gaataacgaa gctatttgca
     7081 gctgatcgtt ctctctttct tagtaagagc aatcaaacag ctgactgaat aatgaaagga
     7141 agactgtatc gtgaaagaaa atcaagaaga actagataaa gaaatgacag atgctcagcc
     7201 agaaccggaa atcgatgtcg aagctgcaga agattcgggt atttcagaag ctgaagcgga
     7261 agaattcgaa acagcgaaat taaaagctga gttagaagaa atggaagata aatacttgcg
     7321 ggctcgtgct gaaatcgcaa atatggcaaa tcgaggaaaa aatgagcgtg agcaattaca
     7381 aaaataccgt tcacaagatt tagccaaaaa attacttcca tcaattgaca acctggaacg
     7441 tgcattagcc acagaagtgt cagatgatca aggggcaggt ttgaaaaaag gcgtggaaat
     7501 ggtcttggaa agtttgcgta acgcactgga agaagaagga attgaaaaaa tccctgctaa
     7561 aggagaagcc tttgatccta atcttcatca agcagtacaa actgttccag cgactgaaga
     7621 tacaccagcc gatacgattg tagaagtact acaagaaggc tacaaattac atgatcgcgt
     7681 gttaagacca accatggtca ttgttgcaca ataattgtgc ggatataaaa cttacacttt
     7741 caattaaaga aggagacgtt aattatgagt aaaattatcg gtattgactt aggtacgact
     7801 aactctgcag tagcagtatt ggaaggaaac gaagcaaaaa tcatcgctaa cccagaagga
     7861 aaccgtacaa caccttctgt tgtatcattc aaaaatggag aaatccaagt aggggaagtt
     7921 gcaaaacgtc aagctgtgac aaacccaaat acgatttcat caatcaaacg ccatatcggt
     7981 gaagcaggtt acaaagtaga tgtagaaggt aaatcatata caccacaaga aatctcagca
     8041 atgatccttc aatatatcaa aggattcgca gaagactatt taggtgaaaa agtcgaaaaa
     8101 gcagttatca ctgtgccagc ttactttaat gatgcacaac gtcaagcaac aaaagatgct
     8161 ggtaagatcg ctggtctaga agtagaacgt atcgtcaatg aaccaactgc agctgctctt
     8221 gcctatggtt tagataaaac agatcgcgac gaaaaaatct tagtattcga ccttggtggc
     8281 ggtacttttg acgtatctat ccttgaacta ggagacggtg tctttgatgt attgtcaaca
     8341 gctggcgaca accatctagg tggggacgac tttgataaca aaatcatcga ctacatggta
     8401 gcagaattca aaaaagaaaa cagcattgac ttgtctcaag acaaaatggc tttacaacgc
     8461 ttgaaagacg ctgctgaaaa agcgaaaaaa gatttatctg gcgtatcaag cacacaaatc
     8521 agcttgccat ttatcacagc aggagaatct ggaccattgc atttggaaat gacattgaca
     8581 cgtgcaaaat ttgatgaatt aacagctgac ttagtagaac gtacaaaagt accagtacgt
     8641 caagcgctaa aagatgcagg tttatcacaa tcagaaatcg acgaagttat tttagtcggt
     8701 ggatctactc gtatccctgc agttgtagaa gcagtacgta aagaaacagg aaaagaacca
     8761 aacaaatcag ttaaccctga tgaagtagtt gctatgggtg ctgctatcca aggtggcgtg
     8821 atcactggtg atgtgaaaga tgttgtttta cttgacgtta caccattgtc attaggtatc
     8881 gaaacaatgg gtggagtgtt cactaaattg attgaccgca atacaacgat tccaacaagt
     8941 aaatcacaag tattctcaac tgcggcagat aatcaaccag cagtagacat ccatgtatta
     9001 caaggtgaac gcccaatggc tgctgacaac aaaactctag gaagattcca attgacagat
     9061 attccagcag ctcctcgtgg tattccacaa atcgaagtaa cattcgatat tgataaaaac
     9121 gggattgtaa atgtatctgc gaaagatcta ggtacacaaa aagaacaaaa aatcacgatc
     9181 aaatcttctt caggtctaac agacgaagaa atcgaacgta tggtaaaaga tgcagaagca
     9241 aatgcagaag cagataaagc tcgtaaagag gaagttgacc tacgtaatga tgtagatgca
     9301 ttgctattct ctgtggacaa aacattgaaa gaattagaag gcaaagtgga cgaagaagaa
     9361 gtgaaaaaag cagaaacagc tcgcgatgaa ttgaaagccg ctgttgaagc aaacaacatc
     9421 gaagaaatga aaactaaacg cgatgcattg aacgaaatcg ttcaaaactt gactgttaaa
     9481 ctatacgaac aagcggcaca acaacaagct caagaaaacc cagaagcagc tcaaggcgga
     9541 gcagatgatg tcgttgatgc tgattttgaa gaagtcgatg gcgacgataa ataatcagtt
     9601 cactgaattt cgagagaagg ccaaagttgc tactttggct ttttcttttg aaaaatagtt
     9661 tgaagtatcg gtttttgttt caaacaaaat atgatatgat aacaagcgac gtgaaaatga
     9721 tgagaaccaa taggaggaaa gcctatggca acaaaacgtg attattatga agtcttaggg
     9781 ctctcaaaag gagcatcaga agatgaaata aagaaagctt atcgtaagct ttcgaaaaaa
     9841 taccatcctg acatcaataa agaacctgat gcggaagaaa aattcaaaga agtatcagaa
     9901 gcatatgaga ttttaagtga cccgcaaaaa cgcgctgcat atgaccaata cgggcatgca
     9961 ggcacagatc caaattacgg tgctggtgga gctggtggcg gatttggtgg attcggcggc
    10021 ttctctggtg gcggcttcgg tggttttgaa gatatcttcg actctttctt tggaggaggc
    10081 ggtcggtcag tcgatccaaa tgcgcctcgt caaggtgctg atttgcaata tacggtcgac
    10141 ttgtcttttg aagaagcgat ttttggtgta gaaaaagaaa tcaagtataa tcgggaagaa
    10201 atctgtcata cttgtggtgg gaacggtgca aaaccaggca cacaaccaac tacttgtcat
    10261 aaatgtcatg gtgcaggtac gattaatgta gaacgccaaa caccacttgg acgagtgatg
    10321 agccgtcaaa cttgtgatgt ttgtcatggg acagggaaag aaatcaaaga accttgtcca
    10381 acctgtcatg gctcaggaca tgagaagaaa gcacattcag ttaaagtgaa tgtaccagct
    10441 ggtgtagaag aaggacaaca aatgcgttta gctggtcaag gagaagctgg agaaaacggc
    10501 ggaccatttg gtgatttgta tgtggtcttc cgtgtagaag aaagtgatat ctttgatcgt
    10561 gaaggttctg aaatttacta tgaattgcct ttaagttttg tacaagcagc tttgggggat
    10621 gaagtccaag taccaactgt ccatggagat gtgaaattga agatccctgc tggaacacag
    10681 acaggtacga acttccgttt acgtggaaaa ggcgctccga agttacgtgg tggtgcaaca
    10741 ggcgaccaac atgtcaaagt aaaactgatc acaccgaaaa atctaaatga acaacaaaga
    10801 gaagcattac gtgcattcgc tgaagctggc ggcatcaaag ttgaagaaca gcaagaagac
    10861 ggtttcttcg ataagtttaa agatgctttt ggcgggaaaa agcggaagta gtttttctag
    10921 taaaaacgct aagtcattaa taattaaaaa ccagacgaac aatcatatgt tcgcctggtt
    10981 tttttgtagt tgtcatgttt gtaccaacaa aaaagccagc tttagaatat gataatcgtc
    11041 atagtttcta ggggtataac cagtgagaga atggaacttg ttcaatcgat attgcaaggt
    11101 gtttttatga ataaataatt cttctgagca atttttgatg cttccgttat ttttactgaa
    11161 gcatgccaaa gtcttcttga acaaatgtac ttcttcttct gtcaggcttt tcaaaatacg
    11221 ttttacatat aatgttcgtt gatctgacgg gattgctggt aaaacgatcc cgaggtctaa
    11281 atcttcaaaa aagcggatct cgttcacaga ttgatggaaa gctatccatt cgcaaacttg
    11341 tttggcatga aggaaagatt gcggtgtctc ttgctctgta taacaaagcg acccaattcc
    11401 aaagtttagc tgtaactgat acattttatt aaactttgtc tggattttat caagcaatga
    11461 tagcaatgag gctctggaaa tccgattgac taggataatg atttgttgtc tatgtacagt
    11521 gagcaaagcc tgtccgtaat aagagaagac cctttcgagc aactccaata aaggattgat
    11581 ccgttcttga cacaattgct gattgtcaga aagttgtccc acaactacat aatatggaga
    11641 agaaaaatca agctcaatta agtgagggtg gtatcgacta tgtacttgat atcatcaatg
    11701 aaccgatggt actttcttat gcgatggaac aggtagagcg attgcttgtt tgtgcaggaa
    11761 aacaagcgat gcggctgata cgattaggtg agaaaataag aagataaaaa tacacggtcc
    11821 cttcatggcg accgtgcatt tttggttttt attaaatatt taatacttta ttcaagaaat
    11881 cttttgtccg ttcattttgc ggattttcaa agatttgttg aggcgttcca tcttctaaaa
    11941 agtttccgtc gtcaatgaac atgacgcgat tggccacttc tttagcgaac cccatttcgt
    12001 gcgtaacgat aaccatcgtc ataccttgtt ctgctaactt tttcatgacg ttcaagacat
    12061 caccgaccat ttcaggatcg agtgcagaag taggctcatc aaaaagcatg atgtcagggt
    12121 tcatcgctag agcacgagca atagccacac gctgtttttg tccaccagaa agcatttcag
    12181 gatacatatc ttttttatct tctagaccaa ctgtctgcag taatttgatt gcttttttct
    12241 ctgcttcttc tctagattct ttcttcaggt ccatcggtgc taaaatgatg ttttccaaaa
    12301 tagaaagatg agggaatagg ttaaagtgtt ggaataccat tccgatatgt tgtctcacca
    12361 agttgatatc tgtgctttta tccatcaagt gggcgccgtc aataataatt tcaccgtcag
    12421 aaggttcttc taaacgattc aagcaacgca agaatgtact ttttcctgaa cctgaagggc
    12481 caatgataca gacgacatct ccttcgttga tggatacatt gatgtcgttt aatactgtgt
    12541 tatcgccgta ttttttgact aaatgctcaa ctaagatttt ttctgccatc ttacttcacc
    12601 ttcttttcta aaacttttgc taattttgtc aatgctgtga tcaaaatcaa gtacatgatc
    12661 gcaatgatca agtaaacata cgtactttgt gttgtacgtg caacaatgat cttaccagtt
    12721 tgcagcaatt cgactacacc gattgcggaa atgatcgttg tgtcttttaa tgagataacg
    12781 aactgattga cgaatgatgg gatcatgatg cgtatcgctt gcggcaaaat gattttttgc
    12841 attgtccgat tataagaaag acctaaactt ctggaagctt ccatttgtcc gacaggaacg
    12901 gcgttgatcc cgcctcgaac aatctctgcg atataagcac ttgcgttaag agtcaaggtg
    12961 ataacaccag caaggaagtc aggaatcgta aatccaacga taccaggcaa cccaaagaaa
    13021 ataaagaacg caagaaccat cattggaatt cctcggatga tatcgacata aatactagca
    13081 attgtgcgaa gagcgcgtat tggtgctaca ctgaataatc caaaaatcac accgattact
    13141 atagccaaag caaaggagat caaagttagg agaattgttc tccaaagtcc tttcaacaat
    13201 gcttgatagt tgttttggat aagtccaaga aatgttgatt catcagcagt ggaagactca
    13261 ctggaagttt ctgaaccatc cgctacatat tctttaataa tcttatcgta ttcgcctgtt
    13321 ctttttaatt ctttcaatcc ttcattgaac atattaagaa gctcaggatt ttgtcctttt
    13381 ttaacggcaa atgcatattt tccgccagat tcttttttga tcggtgtttc caattcttgt
    13441 ccttgtttga tcgcataacc gattaccgga taatcatcca tcattgcatc gattgcgcca
    13501 acttttaacc cgtcgtacaa ttgatcagca gcatcaaaat atttgataga atagccatat
    13561 tcagattcat ggtcgcttaa aaattcagca ctttcggtac caacttttgc tccgactgtt
    13621 tttcctttca aatcttcata agacttgatc gaagtatctc cttttttgac agctaattgg
    13681 atcccgctat caaagtaaga atcagaaaaa tcatagcttt tctttcgttc atctgttaca
    13741 gtcattccgg caatcattcc gtctgcttgt cctgattcca aagcttgcaa agcagcagag
    13801 aaaccacggt agtccattcg gatgttaaat ccttgcagtt tggcgatgtt ttccatcaat
    13861 tttacatcga tgccttcgta ctcgcccttt gcgttttgaa attcaaatgg agcaaaagca
    13921 gagtcactgg caattacgta tacgtctttt ttgggctcta ttttttgcat tgtctcgctt
    13981 gtgcttgact ctgccgttgt agtgttgtca gcttgtgcaa catgaccaaa cgcaaatgtc
    14041 acaagtcctg tcattaagaa aacaaatgag tgaaataaat gctttctttt cataatcaac
    14101 ctcctaaatt tccaatattt tattattata ggcgaaaagt gctgaatcag acaagactgt
    14161 aagctcgaaa aaatgaaaaa aatgcgagtt tctaacattt tatgttataa aacataacat
    14221 gtataataag ataaaaaagt ggcgaaagta tgttcggtgt ggtataattt tttagtaagg
    14281 aatgcctttg ctttcctttt ctttttattg gtcgtaaaat gaaaaggaat gaatgaaaag
    14341 gaggacgaag aatgattgag agagatacct cccgacactt tgagcttgtc tcgaaatacc
    14401 aaccggctgg tgatcagcct gaagcgatta accaattagt ggatggcgta gtcggcggga
    14461 aaaaagcaca aatcttatta ggtgcgactg gaacagggaa aacatatacg atttccaatc
    14521 ttattgagaa agtgaataaa ccaacactga ttattgccca taacaaaaca ttggctggtc
    14581 agctgtacgg ggaattcaag gaatttttcc ctaacaatgc tgttgaatat tttgtttctt
    14641 attatgatta ttatcagcct gaagcttatg tgccttcaag cgatacgtat atcgaaaaag
    14701 attcaagtgt aaatgatgag atcgacaaat tacgacattc ggctactagt tcattattag
    14761 aaagaaatga tgtgatcgtc attgcatccg tttcatgtat ttttggatta ggctcgcctt
    14821 ttgagtacca aaaacaagta gtttcgattc gacagggagc agaactagat cgcaatcagt
    14881 tgattcgtga cttagtcagt atccaattcg aaagaaatga tatagatttt cagcgcggtc
    14941 gttttcgagt gcgcggtgat gtcgtggaga tttttccggc ttcaagagat gaacgtgcct
    15001 tacgtgtgga gttttttgga gatgaggtcg aacgtatccg agaagtcaat gcactgacag
    15061 gtgaagtttt aggagaaacg gaacacgtag caatcttccc ggcgactcac tttgtgacga
    15121 atgatgaaca tatggagcat gccgtagcga atatcaaagc ggaattagaa cagcgtttaa
    15181 ctgtcttgcg aaatgaaaac aaacttctgg aagctcaacg tttagaacag cgaaccaatt
    15241 acgatattga aatgatgtta gaaatgggct acacgtcggg aattgaaaat tattctcggc
    15301 atatggacgg acgtaaagaa ggtgaaccgc cttatacgct attggatttc tttcctgaag
    15361 attttttgat cgtggcggac gaatcgcacg taaccatgcc tcaaatcaga ggaatgtata
    15421 atggtgaccg agcgagaaaa cagatgctag tagattacgg tttccgtttg ccatctgctt
    15481 tagacaatcg gccacttcgg ttagaagagt ttgaaaaaca tgtcaatcag atcatttatg
    15541 tctctgcgac gccaggacca tatgaacatg aacaaacaga taccgttatc caacaaatca
    15601 tccgtccgac agggctgctt gacccagtga tcgaagttcg tccaatcatg ggacaaatcg
    15661 atgatctagt aggcgaaata aatgaacgag tcgaaaaaga ccagcgggtg tttgtaacga
    15721 cattgacgaa aaaaatggca gaagacctca cagattattt caaagaatta gggatcaaag
    15781 tcaaatattt gcatagtgat atcaaaacgc tggaacgaac agaaatcatt cgagatcttc
    15841 gattaggtga atttgatgtg ttagttggaa tcaatttgct tcgtgaagga ttagacgtac
    15901 ctgaagtttc ccttgttgct atcttggatg cggataaaga aggcttttta cgtagtgaac
    15961 gttccttagt ccaaacgatt ggacgagctg ctcggaatga agaaggtaaa gtaatcatgt
    16021 acgcagacaa agtaacagat tctatgcgtc tagcgatgga tgaaacgtca agacgtcgta
    16081 cgattcagca gaagtacaat gaagaacatg ggatcgttcc aaaaacgatc attaaagaaa
    16141 tccgagattt gatttctatt acgaaagaat cggaagatga tacaaaagaa gcggtacaag
    16201 tctcgtacga agaaatgacg aaggaagaaa aagacacgtt attgatgaaa ttagaaaaag
    16261 aaatgaaaga tgcggcaaaa gctttggatt ttgaaacagc cgcgaatgtg cgagatatga
    16321 ttttagaatt aaaagcttca aagtaggagg acaacatggc taacgataaa attacgattc
    16381 atggtgcacg tgcccataac ttgaaaaata tagatgtaac gattccacga gataaattcg
    16441 tggtcgttac aggtttgtcc ggttctggaa aaagttccct agcatttgat acgctgtacg
    16501 ctgaagggca gcggagatac gtagagagcc tttctgctta tgctcgtcag tttttaggac
    16561 aaatggataa gccggacgta gatagcatcg acggattgag tccggcgatt tctattgatc
    16621 agaaaacaac aagtaaaaat cctcggtcga cagtaggtac tgtgacggaa atcaatgact
    16681 atttacgctt gttgtttgca cgtgtaggac atccgatttg tcccaacgat catatcgaga
    16741 ttaccagtca atcagtagat caaatggcag atcaagtaat ggctttgcct gagcgcacaa
    16801 agatccaagt gttggcaccg atcgtggtaa agaaaaaagg acaacataaa aaaatattcg
    16861 aacgaatcca aaaagaggga tacgtccgcg ttcgagtcga tggtgagatc tatgatttaa
    16921 gtgaagtgcc tgagctggag aaaaacaaaa aacatgatat cgcgatcgtc atcgaccgga
    16981 tcattgtgaa agaagggatt cgttctcgcc tatttgattc tttagaggcg gccctgcgat
    17041 tagcagacgg ctatgctctg gttgacgtga tcggtgaaga agaaatgttg tttagcgaac
    17101 actatgcctg tccttattgc ggatttactg ttggcgaact agaaccaaga ctgttctcat
    17161 tcaatgcgcc ttttggagct tgtccagact gtgatggttt aggtgtcaaa ttagaagtcg
    17221 atcaagattt agtgattcca gatagaacga agacacttag tcaaggtgct atcgttccat
    17281 ggaatccaat cagctctcag tattatcctc aaatgttagc gcaggcttgt gaaagtttcg
    17341 gtatcgattt agatacacca ttcgaagaat taccggaaga gcatcaggag atcatcttga
    17401 atgggacaga tgatcttttc catttccatt atgaaaatga ttttggtggc gttcgcgatg
    17461 tagaaacgac tttcgaaggg gtattgaaga atatcgagcg tcgttatcat gatacgaaca
    17521 gtgatttcac gcgtgagcaa atgcgtcttt atatgacaga attaacttgt cagacatgta
    17581 aaggttatcg attgaatccc caagctctat ctgttcaaat caatggaacg aatatcgggc
    17641 aaacaaatga gttgtctatt caaaaagcag tagatttctt ctcaaactta acgttatctg
    17701 aacaagagaa agtaattgcc aaaccgattt taaaagaagt aaatgacaga ctttcattct
    17761 tggaaaatgt gggactagac tatctaactt tgagtcgcgc ttccggaaca ttatctgggg
    17821 gagaagcgca gcgaatcaga ttggctacac aaattggatc gaatctttct ggagttttgt
    17881 atattttaga tgaaccatcg atcggattgc atcaacgcga caacgatcga ctgattggct
    17941 cattaaagaa aatgcgtgat cttggcaaca ccctcgttgt agtcgaacat gatgaagata
    18001 cgatgtgggc agcagattat ttgattgacg tcggacctgg ggccggagaa caaggcggac
    18061 aaatcgtggc tgcaggcact cctgaagaag tagctaatga tgaaaattct ttgacaggga
    18121 aatatctttc agggaaaaaa tcaatccctg taccaaaaga acgccgtaaa ggtaatggga
    18181 aagcaatcaa gatcactggt gcttcagaaa acaatctgaa aaatattagt gttgaatttc
    18241 cattaggaga atttgtagca gtcacaggtg tgtctggttc tggaaagagt acattggtca
    18301 atagtatctt aaagaaatcg ttagcgcaaa aattaaataa gaattctgct aagccaggta
    18361 aattcaagac aatttccggc tacgaaagta tcgaaaagat catcgatatc gatcaaagcc
    18421 caatcggccg gacgccgaga agtaatccag cgacttatac aagtgtattt gatgatatcc
    18481 gtgggttatt tgctcaaacg aacgaggcaa aaatgcgggg ttataagaaa gggcgtttta
    18541 gtttcaacgt aaaaggcggt cgttgtgaag cttgtcgcgg ggatggaatt attaagatcg
    18601 aaatgcactt tttgcctgat gtctatgttc cttgtgaagt atgtcatggt aaacgatata
    18661 attctgaaac attagaagtg cattacaaag gaaaaagcat tgctgatatt ttggaaatga
    18721 cagtagagga tgctgtagaa ttcttcaagc acattccaaa gattcatcgc aaactgcaaa
    18781 cgattgttga tgttggctta ggttatgtga cgatggggca accagcaacg acattgtccg
    18841 gtggtgaggc acaacggatg aaacttgcca gtgaattgca caaaatctct aatggaaaga
    18901 atttctatat actagatgaa ccaacgacag gacttcatag cgatgacatc gcccgcttgt
    18961 tgcatgtatt acaaagatta gtagatgctg gtaacacagt tttagtgatt gaacacaatc
    19021 tagatgtaat caaaacagca gattatatca ttgatttagg accagaaggt ggagaaggtg
    19081 gaggaacgat ccttgcgact ggaacaccag aagaaatcat taacgtaaaa gaaagttata
    19141 caggtcacta tttgaaaaaa ataatggtat aaagaggata gatagctgag atagttaaaa
    19201 aaacataaaa aaatgaattt ttgcttgttt tattaatatc agtgttctag aatcattaca
    19261 tggtagttct ttctacactg attggtatag ataaaaactt cctataagtt caatccgttc
    19321 aaaaaagaat tgagcaatcg gaggactttc ctttgattgc ttttttcttt atggtatgat
    19381 attccttgtg tagttggaaa agaaaaacaa gagattatga gtaagtgaga gataatgatg
    19441 aacagtattg aaaatgaaga atttatccct atattattag gaagcgacat gaacgtttat
    19501 ggtatggcac gttcttttaa tgaagcatac ggcaaaatct gccaagcata tgcttcggat
    19561 cagttagccc ctacccgcta cagtaaaatt gtcaatgtag aagttattcc aggatttgat
    19621 aaagaccctg tatttatcga gacgatgctt cgtttggcaa aagaaagata ttctgataag
    19681 agtaaaaaat atcttttgat tgcttgtggt gatggatatg cagaattgat ctcccaacat
    19741 aaacaagagc tgtcagaata ttttatctgt ccatatattg attattcttt atttgaaagg
    19801 ctgatcaata aagtaagctt ttatgaagtt tgtgaagaat atgatcttcc gtatcctaaa
    19861 acattgatcg tcagagaaga aatgttagta aatggacatt tggaacagga attgcctttt
    19921 gaattcccag ttgctttgaa acctgcaaac agtgtggagt atctttctgt ccaatttgaa
    19981 ggaagaaaaa aagcttttat tttagagact agagaagagt tcgatttaat tttaggccgg
    20041 atatacgaag ccggttataa aagtgaaatg attgtacaag attttatccc gggtgatgat
    20101 agtaatatgc gtgtgctaaa tgcgtatgta gatgaagatc atcaagttcg gatgatgtgc
    20161 cttggtcatc cactattaga agatcctaca ccagcatcga ttggtaatta cgttgtgatc
    20221 atgccagatt ataacgaaaa aatctatcaa accattaagg catttttgga aaaaatcgaa
    20281 tatacaggct ttgccaattt cgatatgaag tatgatccta gagatggaga atataaatta
    20341 tttgaaatca atttgagaca aggtcgtagc agtttctttg tgacattgaa cggcttgaat
    20401 ctggctcgtt ttgtcacaga agatcgagtg ttcaacaaac cctttgttga aacgacctat
    20461 ggaacgaatc aatcagataa agcaagatta tggatgggtg tgcccaaaaa aatcttttta
    20521 gaatatgctc gggaaaatga agacaaaaaa ctagctgagc aaatgatcaa agaaaatcgt
    20581 tacggtacta ctgtatttta tgaaaaagac cgatcaatca agcgctggtt gttgatgaaa
    20641 tatatgttcc ataactatat tccaagattc aaaaagtatt ttcatgtgaa agaaggctag
    20701 ttcatggaga attttttcag tattttaggc ggaatgggca cgatggcgac agaaagtttt
    20761 gttcgcttga tcaaccaccg aacgaaagct acaaaagatc aagaatattt gaattatgtc
    20821 ttattcaatc atgcaacagt tcctgatcgt acagcttata ttttagatcg atcagaagaa
    20881 aatccaatgc cgtttctgct ggatgatatt gagaaacaaa atttattgcg accaaacttc
    20941 attgtgttga cttgtaacac cgcacactac ttttttgaag agttacaagc ggcaacggat
    21001 attcctatct tgcatatgcc aagagaagca gcaaatgaac ttgtccgtca gcatacgaca
    21061 gggagagtag cgattctagg aacagaagga agtatgaaag ctggaatcta tgaacgcgaa
    21121 gtcaaaaatc ttggtttcga aacagtgatt cctgatactg cgcttcaaga aaaaatcaat
    21181 tacctgatct atcatgagat caaggaatcg gattatttga atcaagagct ctattacgaa
    21241 atcttagagg aagcggtaga gcgcctaaac tgtgaaaaag tcatcctagg ttgcacagaa
    21301 ttatctttga tgcatgaatt tgctgaagat aatcattacc cagtgatcga tgctcaatct
    21361 attttagcgg atcgaacgat cgaacgtgca ttagctgaaa gaagcgaggc attggataca
    21421 gcatcggaaa agtaaaaaaa ttggatttgg ggcaaatatc ctaaaagata tttggtccaa
    21481 atctttttgt aaaagaaaaa gaaaactggt aatgatacgc ccgggatgat ataattttat
    21541 tatgcacaat gtgagagggg ttcgtaaata tgacagataa tttgcaatta gtcatcataa
    21601 caggtatgag tggtgcaggg aaaacagtag ctatccaaag ttttgaagat atgggctatt
    21661 tttgtatcga taatatgccg cctcgattga ttccaaaatt ctgggaattg atcaaagaat
    21721 caggtaaagt cacaaaaatg gctttaggag tggatttgcg ttctcgttcc ttctttgaag
    21781 aaatccaaaa catgctgatc gaaatcgaaa atacgaactt catcgacact tctatcttat
    21841 ttttagatgc ttcagacgaa gaactagtgg ctcgctacaa agaaacaaga cgagcacatc
    21901 caatggcgat ggacggtttg atcactgaag ggatacgtaa agaacgggcg atactagacg
    21961 acttgaaagc ccaagcttcc gtgatcattg atacgacaac attgacacca cgacaattac
    22021 gtgaaaaaat caatgagtca ttcaaatcaa agggagacca aggatttcgg atcgaatttg
    22081 tatcatttgg ttttaaatac gggttgccga tcgattcgga tattgtgatg gatgtgcgtt
    22141 ttttgcccaa ccctcattat atcccggaac taagaccatt gaccggaaaa gatcagccag
    22201 tctatgatta tgtgatgtcc tttgatgaaa cgcaatcatt ctaccataaa tttttggatc
    22261 ttctggaaac tgtattgcct ggatacatta aagaaggaaa aagcagcttg acagttgcaa
    22321 tcggctgtac aggcggtcaa caccgttctg tggcattgac tgaacgtttg gcaaatgctt
    22381 tagggaaaaa ctataaagtc aatgtgacac atcgggacaa ggataaaagg aaagagacgg
    22441 tgaaccggtc gtgaaaatga aaacctaccg gataagaaaa ccaaagatcg tagtggtcgg
    22501 cggtggcact ggtctgccag ttatcctgaa aagtttacga aatcaaggag cagatatcac
    22561 agcagtagtg actgttgcag atgatggtgg aagcagcggt gcaatcagag aatcgatcgc
    22621 tatggctcct cctggtgact tgagaaatgt cttagtggcg ttatcggata tgccacaatt
    22681 ctatgaagat attttccaat accgtttcaa aaaggaagat cagtttttag ccaatcatac
    22741 gatcggtaat ctgatcattg ccgcagtttc cgagatgcgg ggaagcactt atgaggcgat
    22801 tcaattatta gcaaaaatga tgcatgtaga tggacatgtt tatccttctt cagaaactcc
    22861 tttgaccttg catgctgttt ttaaagacgg aacgactgca gtaggagaat ctaaaatcgc
    22921 gatcaaccgt aaaaccattg accgtgtgta tgtctgcaac acaaatgata aaagtgaagc
    22981 caaagcggct cgaaaagtcg tttcagcaat tatggacgcc gacatggtcg ttttgggacc
    23041 cggaagttta ttcaccagta tccttccaaa tttagtgata ccagaaatcg gcgaagcaat
    23101 gctcaagaca actgctgaaa ccgtctatat ctgtaatatc atgacccaaa aaggggaaac
    23161 ggaacatttc acagatgccg atcatatacg tgttttacac aagcatttga atcagccttt
    23221 tatcgataca gtattggtca atacggaaaa agtgcctgaa gattatatgg acccagagat
    23281 ctatgatgag tatctagtac aagtcgcaca cgacttcaac ggattgagaa atgaaggatg
    23341 tcgagtgatt tccaccgact ttttagaatt gaaagatggt ggtgtcttcc acgatggaga
    23401 caaagtagta gaagaattgt tccgtttagt tttcggttca aaatattaag caatttattg
    23461 ttggagaaag gaggagaata ggatgtcatt tgctgcagat gtgaagaaag aactaactgg
    23521 tttggaagtt catcgagaac atgcgaaagc ggaattagct gctttgctgc gaatgaacgg
    23581 ctctttaggt attgtgaacc agcagtttat tttgaacgtc caaacagaaa atgctgcaat
    23641 tgccagacgt atctattctc ttttgaaaga tcattatcag gtacgaagtg agctactcgt
    23701 tcgtaaaaaa atgaaattaa aaaagaacaa tgtctatatc gtccggttga agcaaggcac
    23761 aaaagatgtg ttgatggatc ttgatatcat ggatggtatg atgttcaatg gacacgtttc
    23821 ggacgagatt atgggaaatt cgcaaaaaat gcgttcttac ttacgtggtg cttttatggc
    23881 ctcaggttca gtcaataatc cggaaacgag tcgttatcat ttggaaatat tttctattta
    23941 tgaacagcat aaccaagaca tttgcgatat gttaaattat tacggtctac atgctcgaac
    24001 gcttgaacgg cgcaatggct atatttcgta tttaaaagga gcagaaaaaa ttgctgattt
    24061 cttaacattg attggtgcaa ccaactcaat gctccgattt gaagatgttc gaatcgtacg
    24121 ggatatgaga aattcggtca atcgtctagt caattgtgag acagcaaatc tgaacaagac
    24181 gattgatgcg gcttctaaac aaatcgaaaa catcgaattg atcgaagcaa aggtcggtct
    24241 acatgccctt cctgaaaaac ttcaggaaat cgcagaactt cggcttcaac atccagaggt
    24301 cagcttgaaa gaattagggg aaatgattcc ctcaggtgca atatcaaaat cgggaattaa
    24361 tcaccgaatt cgcaaaataa atgaatttgc ggaaaaactg cgtgaagaag cagtttaatc
    24421 caaagaaaaa actgatacat gaagcaggag atgcgaatgt atcagttttt tttaaaagct
    24481 atacttgctt ttcttccgtc aaactataat tccaacttta ctggagtaac ataatgttga
    24541 aaggcttcta ttgtttttta atacaatcgt ataaaaagcg gggagacaac tgaatgaaga
    24601 ttttagtttt atacataaac ttttgcttaa tatagaagtc ctgatataga taggatcaag
    24661 tatttatcta taactggaga ttaattaaaa actggaagga actgctcgat caaccttgag
    24721 aactgttttt cttacttctg gtcaagattc tcgtggttgg tggataagga tggttgataa
    24781 gttcattgga ttagctagtc gaaaacaacc aggtgtaatt aatgtagaag ttttaacaag
    24841 gggatggagt gatttattca gtgataatca taagttcata aatagtaatt gggatgtatg
    24901 gttggtatac gttatacctg aacttgaact atcaagattt ggtttagaat ttgagaatcg
    24961 atgtttataa agggagggca aaaaatgtgc ttttttacaa cttttactat cattatgatt
    25021 gcagtacttt ttatttttct agatatagca aagcgaaata cagcattttt attatatcgg
    25081 gttttacttc gagcaggtct cataacattt atatcgatcg ttggattttt tctattcaca
    25141 gttattgtat ttatttggag aactacagca ccaccattgc cagagattac atatggagaa
    25201 tttccgttta gtcttgaata tgaaatagat gaggaaagaa ttttgattga agatacggtg
    25261 attgcagaat ttataggaag ttttagaggg aatgtgacaa ctcaacctag acgaagttgg
    25321 agtataaaac ttttaaataa tagtggagag ttgaaagcat ttcattatga attcttaata
    25381 atagaaagtg aagatgtaac aatcacttta agtagtggct ctgctggata ttttatgaat
    25441 gatggtggaa gcaagccaca gctcacagtc cgtgatcgta ttgcaggttt tatgcatttt
    25501 tctcactatg acttagaaga agctcgtaaa atacttgcag aatatgggat cacattaatc
    25561 aattgggaat atgcgccacc gataagaaat tttttcaatg attattatta gtcttataaa
    25621 aaacagaatg taaaatttat gtgcgcgagt atttagtttt ccattttttg tttgatagat
    25681 atatagcatt gttgaagcat gggatagtgg tgccaatttt atcaatgagg ttggtcaggc
    25741 agttaacata attggtgatg ctataaatcc atttaattgg aattggagga attaggtgtg
    25801 gaaacagtga gattatggat attcatgaaa aattttcgtt cggctttgag atatgttatt
    25861 ctggaatcaa aatcaggtta ttatctgatt gacacagatc actatacttt ttggggaggc
    25921 tacctttttc cgatttttaa ttggataatc ccccataagg ttatgaaaat taacttgcct
    25981 gcagaagaaa taaatagatt aatactcaac aaagatttac aaaggaaaag agagaaagaa
    26041 cattctaatg ttgcttggat aggtccagca tttgcagtag tgacttcttc aattgtcggt
    26101 cctatgctag agagtattta tagtgatttt tctattggaa tacgttattt atttgttact
    26161 ttaagtatag ctgttctttt gtgcataaag ggttatgtta gcaagaagaa taaagatatt
    26221 ttaacactaa tcggtgtaaa caatttatct agtactaaaa ttatactgtt tcctgaatct
    26281 ataaaagaag gatcaaggtt gttgataatt tttataatgc ttatactgtt ttctgtttta
    26341 tttggggtga tgtttata
//
LOCUS       DAPCMT010000024        25918 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPCMT010000024 DAPCMT010000000
VERSION     DAPCMT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.024"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..200)
                     /locus_tag="RD743_000844"
     CDS             complement(<1..200)
                     /locus_tag="RD743_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0562700.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(505..1737)
                     /locus_tag="RD743_000845"
     CDS             complement(505..1737)
                     /locus_tag="RD743_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDU0562701.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1991..2560
                     /locus_tag="RD743_000846"
     CDS             1991..2560
                     /locus_tag="RD743_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0562702.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2738..3178)
                     /locus_tag="RD743_000847"
     CDS             complement(2738..3178)
                     /locus_tag="RD743_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDU0562703.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3336..4100
                     /gene="map"
                     /locus_tag="RD743_000848"
     CDS             3336..4100
                     /gene="map"
                     /locus_tag="RD743_000848"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDU0562704.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4132..5055
                     /locus_tag="RD743_000849"
     CDS             4132..5055
                     /locus_tag="RD743_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDU0562705.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5131..6270
                     /locus_tag="RD743_000850"
     CDS             5131..6270
                     /locus_tag="RD743_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDU0562706.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6263..7063
                     /locus_tag="RD743_000851"
     CDS             6263..7063
                     /locus_tag="RD743_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDU0562707.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7063..7890
                     /locus_tag="RD743_000852"
     CDS             7063..7890
                     /locus_tag="RD743_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDU0562708.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7868..8602
                     /locus_tag="RD743_000853"
     CDS             7868..8602
                     /locus_tag="RD743_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDU0562709.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8702..9568
                     /gene="rfbA"
                     /locus_tag="RD743_000854"
     CDS             8702..9568
                     /gene="rfbA"
                     /locus_tag="RD743_000854"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDU0562710.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9582..10154
                     /gene="rfbC"
                     /locus_tag="RD743_000855"
     CDS             9582..10154
                     /gene="rfbC"
                     /locus_tag="RD743_000855"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDU0562711.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10176..11204
                     /gene="rfbB"
                     /locus_tag="RD743_000856"
     CDS             10176..11204
                     /gene="rfbB"
                     /locus_tag="RD743_000856"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDU0562712.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11302..12153
                     /gene="rfbD"
                     /locus_tag="RD743_000857"
     CDS             11302..12153
                     /gene="rfbD"
                     /locus_tag="RD743_000857"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDU0562713.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12188..14221
                     /locus_tag="RD743_000858"
     CDS             12188..14221
                     /locus_tag="RD743_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562714.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14265..15545
                     /locus_tag="RD743_000859"
     CDS             14265..15545
                     /locus_tag="RD743_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDU0562715.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15755..16561
                     /locus_tag="RD743_000860"
     CDS             15755..16561
                     /locus_tag="RD743_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562716.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16573..17793
                     /locus_tag="RD743_000861"
     CDS             16573..17793
                     /locus_tag="RD743_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562717.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17783..19369
                     /locus_tag="RD743_000862"
     CDS             17783..19369
                     /locus_tag="RD743_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDU0562718.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19408..21546
                     /locus_tag="RD743_000863"
     CDS             19408..21546
                     /locus_tag="RD743_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDU0562719.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(21914..22897)
                     /locus_tag="RD743_000864"
     CDS             complement(21914..22897)
                     /locus_tag="RD743_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDU0562720.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23275..24666
                     /locus_tag="RD743_000865"
     CDS             23275..24666
                     /locus_tag="RD743_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDU0562721.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24682..25722
                     /locus_tag="RD743_000866"
     CDS             24682..25722
                     /locus_tag="RD743_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDU0562722.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25741..>25918
                     /locus_tag="RD743_000867"
     CDS             25741..>25918
                     /locus_tag="RD743_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDU0562723.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRI"
BASE COUNT         8223 a         4380 c         5283 g         8032 t
ORIGIN      
        1 tattttgtat aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg
       61 ctaagtatat ttactccttg ataaagtaaa atagatgcat gcgtatgtct tagtccatca
      121 ataaacagtc gaagagaaaa tagggaaaaa ttgattacta aagaaaaact tactaggtcg
      181 acggtgtagt tgattttcat acttcaatag attcgaatta aacgatttcg attggcttat
      241 accagtccta tccaacgaaa gatatatcgt tcttaactct agcggtataa cttgaagctt
      301 tacgtaaagc caatgggaat gaactggaga tgacttttta ttatttttag taatagctga
      361 tgggttaata cttacaactt tccttttcaa agtaccatat aaaaaaacgg tggggttgtt
      421 tagcgaaata aaggatctaa gccaatagct tagatccttt attctatgct ttatattatt
      481 ttttgcatgt gtgcgaaagt atactcaagc ccaatcgcca tttcggaaaa tcggtactgt
      541 agagccatct tcacggattc cgtcgatatc catcttgtca gatcctacca tgaaatctac
      601 gtgggtttga cttctattca atccagcttc tgctaattct tcatcggaca tttctgtacc
      661 gcctttcaca ctgaaggcat aagcagaacc tagtgctaag tgattcgatg cgttttcatc
      721 aaataatgta ttgaaaaaga tgatgccaga ttgagaaata ggtgaagggt caggtacaag
      781 tgcgacttct cctaaacgac gcgcaccttc atctgtatca agcagttttg ctaagacatc
      841 ttctccttgt tcggcagaaa aatcaacaac ttttccatct ttaaaagtaa atttcatacc
      901 cgagatgatg gtgccagcat aactcagtgg ctttgtactt gaaatgtaac catctactcg
      961 atgactgtca ggtgctgtga acacttcttc cgtcggcata tttgccatga atttttctcc
     1021 acgagcatta tagcttccag cgccttccca aagatggttt ttaggcaatc cgataatgat
     1081 atctgtaccg ggagcagtat agtgaagggc agaaaattgt tctcgattca attcttctgc
     1141 tttttttgct aatttttcat catgcttctt ccatgctaag acagggtctt cttcatagac
     1201 acgagtcgtt ttgaaaatct gatcccataa tgcctcaact tgtttttctt ctggcagttc
     1261 tgggaagact tttgctgccc attgtttacc agccgcagcc acgacagtcc aactgacttt
     1321 atttgcttga gttgccttgc gtaaattcat tagtgctttg ccattcgctg attgataaga
     1381 cgctactcga tggctatcta caccagcaaa tgcatcggga tcggcagaaa caacgctgat
     1441 cctgcttgct cctttttcaa gccattcatc cgcttgatcg attttagatt gtggtacatt
     1501 ctcaatacga tccgttgcag catgtaaaag aaattctctt tggatctggt cgtcagtcca
     1561 ttgaacgata acctcagcgg ctcctagttt ataagcttct tgtgtgatca atcgagcaag
     1621 gggtgcctgt tccacgctga tctgtaaaac aactgtgtgt cctttttctg tggctacgcc
     1681 agtttctgca attaagcgtg catatttttt tagaagttca ttaaaatcag ataacataat
     1741 aaaaattcct cctcaccatt ttttctggta actattagca tgataccatt taattatgat
     1801 tcaaacaatg aggctttaaa taaaaaaagg aggttatttt tttaaggtta agtttcatga
     1861 tatatgttac tacttgctat ttcagcttgt gaagaaaatc aaaaactaat tttgcatatc
     1921 aaaaaaacat gttacaatga aattaatcaa tggtggaagc gttataaaaa aattaaggag
     1981 attaaaaaat atggcaagaa aaaagacgat tactagagaa cagattttgg cagctgctta
     2041 tgaagtagct gccaccgatg gatttacacg atttactgcg agaaacatcg cgaacaaaat
     2101 gaattgttcc acgcaaccga tttatcttga gtttaaaaat atggatgact tgagacaagc
     2161 tctttttgag cagatctatg atcatttaca atatgaagtg ttcactgttg agcatacagg
     2221 gaatacgatt atcgatcttg cttttaatta tatccatttt gcaagaagag agaagaagtt
     2281 gtatcgttca ctgtacttag aagaatatgg tggaggaaaa gagatgcaga atttctctta
     2341 tacttacttt gtgaatacag tgaaacgcga tccagcttat gtgaatcttt cagaagagca
     2401 gatcgaatct ttgtataatg ggacatggat catagctact gggatcgcag ctttgatgtc
     2461 ttcaagcatc atccatccaa ctgatgaaca gattgaaaag atgatccaag acagtataga
     2521 tgctatcttg aaattcaaag aaccaatcga aatcgattga atatactcaa aagttccaca
     2581 aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag ttcgtttttc ttacagcgta
     2641 tgcattttta actcccgaag aactcttcca tgaatgcaat gaataaaaga gccatacaga
     2701 ttgaataaat ctggatggct cttttattgc aagtatatta aatttttgcg actagttctt
     2761 tagcgaaagc ttctaaattt tcgatatctt cttcttcagc attcagatcc actttaacat
     2821 tttcggctcc ttttgatgcg cctgtttttg caaaagcagc atcgaaatca tccaccgatt
     2881 tgcagaattc atcgtaaaaa gtatcaccag atccgcagac accgtaaatt tttcccaata
     2941 gatccaattc ttgcaagtct tcataaaagt ctacgatctc atcaggcaga tcgccatcat
     3001 cgtatgtata agtagctact acgcaaatat ctgcctcctc gaattcatct gcatctactt
     3061 gggtacattc gttgatttcg acttcgatat ccagattttc aaatgcttct gcaacaatat
     3121 cggcgatttc ttcagtgttt cctgtcatgc tagcatatac gatttttgct aaagccatga
     3181 tgtgcctcct cgtttataat tatttaacaa ttatgaatta taccaaaaca tcattgaaaa
     3241 gaaaataaaa cctaaagaca cctggggaat gtcatttggc taaatgtatg ccgctatgtt
     3301 aaaatgagag ggatttgtaa aaaggagacg aaaatatgat tactttaaaa tcagcacgtg
     3361 aaattgaaat gatggcagaa tctggagctt tattagcaga tgtacataaa aatctgcgtg
     3421 attttataaa accaggaatc acaagttggg atattgaggt atttgtcaga aactatatcg
     3481 agagtcatgg cggaattgct gcgcaaattg gctttgaagg gtatgaatat gcgacttgta
     3541 tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa aaagcctttg aaaaacggtg
     3601 atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg catttctgat tcttgttggg
     3661 cctatgtagt tggagaatct acacctgaga tcgaccgatt gatggaagta acgaagaaag
     3721 ctttgtacat cggtattgag caagcacaag tagggaatcg gattggtgac atcggtcatg
     3781 ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt tcgtgatttt attggtcatg
     3841 gcgttggtcc tacgattcat gaagaaccag cagttcctca ttacggagaa gctggaaaag
     3901 ggttgcggtt gaaagaaggc atggttatca cgatcgaacc tatggttaat accggaactt
     3961 ggaaaatgaa aatggatcct aacggctgga ctgcgtatac aagagacggc ggactttctt
     4021 gccaatatga acatacattg gctattacta aagatggtcc aagaattttg acatcacaag
     4081 gagaagaagg cacttattga tctatccagt caataggtac gggagaggta catgaaagtt
     4141 tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta ttgagacgac tcaaaagcgc
     4201 atcactgatt cagaaatgag tacgacctct gtcgtcgttg cttattattt actcttgtct
     4261 ttatttccat tgattattgc ttttggtaat attttaccgt ttctccacat cgatcaagag
     4321 actgtactga cctatttacg agaagtgatt cctgagaccg tttaccagtt tatcggtcct
     4381 gcaatcaaag accttttgac acaaagttca ggaggcttgt tatccgtttc tgcattagcg
     4441 gctttatggt ctgcaagtca aagtatcaat gctttgcaaa ttgcgatgaa taaagcgtat
     4501 ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct tctcactaat tgtcattgtc
     4561 ttgttcatga ttgctattag tggggtaact atcgttcttg ggttgggaca gctgattttg
     4621 gatgctattc agccaatcgt acaaataccc gtgaacttta tcgatcagtt ccaaactttg
     4681 aagtggccaa tcacattggt agctttattt gtcatcatgt tcttgattta tttaattgtc
     4741 cctaatgctc agttgaagtt gaagacgatc attccgggca cgatcttcgc tactgtcggc
     4801 tggatgctgt tatcacaagt gtttagtatc tatgctaggt attttgcaac gaggatcagc
     4861 ggttatcaga tcataggaag ttttattgta ctcatgcttt ggcttaattt tgctgctacg
     4921 atcatcattt tgggcggaat catcaatgca gttgttcaag aatacgtgac gggtcatcaa
     4981 atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata aattgaaaac taaattttct
     5041 cataaaaaaa agtagaaaca agagttctta ttgtaaattt atatcagtaa tttataataa
     5101 ttatgactct taattaaagt aggtgcccat atgcttgttt cttttagtat ggttataaga
     5161 ttttttctga caattttatt atcactgata ttaacacctg tattcaaaat catttcagta
     5221 caaacaggta tggtcgacaa accaaatgaa agaagaataa ataaagttcc aatgccttca
     5281 gcgggcggac tgcctatctt tatttcattt gttatatcga ctttattctt attccgtaaa
     5341 attatccctc aatcgtatat tgtaccgatc ttgattgctt ctttagtaat catagtcaca
     5401 ggagtgctcg atgataaata tgagctgaca cctaaacaaa aaagtatagg gatcttgatt
     5461 gcttctctta ttatttattt tgtagcagat atacgtattg attccttcac gcttcctttt
     5521 attggatata tccaactagg ctggcttagt tttcccgtaa cgattttttg gatcttcggt
     5581 atcacgaatg cggtcaatct gattgacggt ttggatgggc tggcggcagg gatttcattg
     5641 atcggtttaa ttacgatagg aatcattgga tattttttcc tccatgcttc tacagtgtat
     5701 attccagttg ttattttctg tcttgtagca agtattatcg gtttttttcc ttacaatttt
     5761 tatccggcaa agatttattt aggcgataca ggagctctat tcctagggtt tatgatggcc
     5821 gtcctttcac tacaagggct gaagaacgtg acttttgttt catcgatttc tttgctgatc
     5881 gtcatgggag tgccggtaac agacacgttt tttgctatca ttaggagaaa agcaaaccgt
     5941 gtttcttttt ctacagcaga taaaaaacat cttcatcacc gactaatcag tttaggcttt
     6001 actcataaag gtgctgtcct gacgatttat gcgatggctt tgatgttttc tttcacagct
     6061 atggtcatga attatactgg aagaatcgga acgatcgtat tgattattgc catgttgttt
     6121 gctgcaattt tattatttga attgattggt ctgatcaatg agaaacatca gtttatttta
     6181 aaaacattgc gatttttagg gaataaagag taccgtacgc aagtaaaaca gaaatatttg
     6241 aagaaatgga accgtcatat agatgaataa caaattaaac cagccgatcc ttattagtat
     6301 tgtgacggca aacagtgata aaatttttcg gacactagat aattttattt caactgtaaa
     6361 agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat tcacttcccg agtacaaaaa
     6421 aagactaaca gagtatagga aatatcccta catcgatatc tattttcatg atgaaaattc
     6481 cggatttggt tatggacata atcacaatct gatagctagc gattctcgct atggcattat
     6541 ttgtaaccca gatattttag tggatgaaga gacagttaca gcattggtct tgttattgaa
     6601 agaacatccg gaatgtgcga tgttagcacc taagattctt aatgaagatg gaacaactca
     6661 gcacctcatc cgtgagcagc tggctgtttt tgattacgtt ttgcgctttg tgcctttcaa
     6721 atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt gaatgccgag gattgcctac
     6781 tgatgctaat agttatgtac ggatgatctc aggtagtttt atggtagctg atattgataa
     6841 atttaaagag ataaaggggt ttgatgagcg tttcttcatg tactttgaag acaacgactt
     6901 gtgtttgagt tttgaaaaag caggttacaa gttattgtac acaccctttt atgcagttat
     6961 ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg tttcgcgtgt ttttgacttc
     7021 tatgatgaaa tttttcaata agtgggggtg gcgttttttc tgatgaaacc agaaatatta
     7081 gtagtcgttg tgctttatca gcaactattc agtcaatctc cagcttatgg accattaagc
     7141 aaagcactta gcgaaaagca aattcagctt attatttacg ataatagtcc tcaaaggcag
     7201 caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc atgatccgca aaatcctggt
     7261 ttagcggtgg cttataattt tgcgttgtct caagcagatg aaggaacacg ttgtctcgtc
     7321 actctggatc aggatacgag gctagtcgac gattattttg aactattgcg taaagtaacc
     7381 tttacagatg aatgtgttgc tgcagttccg atgatttttt ctgggagccg acaagtttct
     7441 cccctatatt cagatcgtta tatcaatcgt acagctcaag cagtagaagt tgaaggccca
     7501 acagctgaac gaatcatggc aatcaattca ggtgttgcct ggtctgtcaa atttctaaaa
     7561 aagattggcg ggtttaatcc tgctttttct ttagattttt tggatcattg gctgttttgg
     7621 aaagtggatc agttggaaaa aactgtggag atccttcctg tacgaatgga acatgatctt
     7681 tcggttttag actatgaaaa agtttcaagt aatcgatacc gttctatttt acaagcagaa
     7741 aatcgatttt atcatgaata tgatcaaagg catttatcca gtcaccgacg acaattattg
     7801 ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc atatctggcg gatgactttg
     7861 cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt tcggtttgta tagctacctt
     7921 taatggaggc agatatttaa gagagcagtt agatagtatt ttatctcagc tttcagcaga
     7981 agatgaagta gtcatttctg atgatgggtc agtagatgat acatgggata tattaactga
     8041 atacgcagaa aaagataagc gaatccagct tttttcgggg cctcgtcaag gattgatcgc
     8101 taattttggt tatgcgatcc agcagacgaa aggagatcta atctttttgg cagaccaaga
     8161 tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac tattttgagc agaatccgga
     8221 tcagctagta gtagtttctg atttaatcat tgtggacagc gagctgcaag agatccatga
     8281 ttcgtatttt tcgtttcgaa attccaaaga aggatggctt aataatgtga taagaagtca
     8341 ctatattggt gctggtatgg ctttccggtc ttctttaaaa cccgatattt tgccgatccc
     8401 gtcagaagtg ccgatgcatg atatgtggat tggtttgctg ggaggaaaga agactgggtt
     8461 cctaagagaa ccgttaactc tgtaccgaag acatgattta aacgcgagtg agattgaaac
     8521 acattctaaa ttacggcaaa aaatttcttg gcgtgttcat ctgattacgg cgttgatcaa
     8581 acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc ttaatatttc catgacaaat
     8641 ataacaattg atgagataat aacaaagtga aatttgaaat caaaaaggag agcagatatc
     8701 catgaaggga atcattcttg ctgggggcag cggaacacgt ctgtaccctt taacaaaggc
     8761 aacatcgaaa caattgatgc cgatttatga caaaccaatg atttattatc caatgtctac
     8821 tctgatgttg gcgggaatca aagaaattct gattatttca acgccacaag atacaccacg
     8881 tttcaaggaa ttatttggaa acggaaatga tttaggactg cagatcgaat atgcagtaca
     8941 agaaagtcca gatggattgg cacaagcgtt tatcattgga gaagaattta ttggagatga
     9001 tagtgtctgc ttggtattag gggacaatat ttactatggt ggcggtctgt ctaaaatgtt
     9061 gcaacgagca gcttcaaaag atacaggcgc gacagtattt ggctatcatg tcaatgatcc
     9121 agaacgcttt ggtgtagtag aatttgatga gaatatgcaa gctttgtcga ttgaagaaaa
     9181 gcctgctcag ccaaaatcaa attacgctgt aactggcctt tatttttatg acaatgaggt
     9241 aatttctatc gctaaaggta tcaaaccttc agaacgtgga gaactagaga ttacggatgt
     9301 caataaagca tatttggata aaggaaaact atccgtagaa cttatgggac gcggatttgc
     9361 ttggcttgat acaggaacac atgaatcatt gcttgaagca tctaccttta tcgaaacaat
     9421 tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa attgcttacc gtatgggcta
     9481 catcgataag gaacaattgc tggcccttgc acagccattg aaaaaaaatc aatacggtca
     9541 atatttattg aatttggccg ctgaataagg agtccttaca gatgaaagta acagatacaa
     9601 aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg cgatcatcgc ggtttcttca
     9661 cagaaagtta ttcaaaagca aaatttgcag aacatggatt gaaatatgac tttgttcaag
     9721 acaaccattc actatcagct gaagctggtg tccttcgggg gcttcatttc caaaaaggag
     9781 aagctgcaca gacaaaattg attcgggtag tcactggagc agttttagat gtagtagtgg
     9841 atatccgcaa aggtagcccg acttatggtc aatgggacgg atttatctta tctgaacata
     9901 atcatcgaca gttgcttgta cctaaaggat atgcccatgg attcgttact ttgacaccaa
     9961 atgtgaactt cctttataag tgtgataatt actataatgc agaagcagat gggggcatcg
    10021 catttgatga tccagattta gcgattgatt ggccaattga tccatcaaga gcgattatgt
    10081 cggaaaaaga tcaccgtcat ccgacattaa aagaatttga agcagaaaat ccatttgttt
    10141 acggagaaat ctaagaatta aggagaaatt ttcttatgaa aaacatcatt gttaccggag
    10201 gtgccggatt catcggctct aatttcgttc attacgtggt aaacaatcat ccagaagtgc
    10261 atgtcactgt tttggataaa ctgacttatg caggaaacaa agagaacttg gccggacttc
    10321 ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga tgcagaatta gtggaccgtc
    10381 tagtaaaaga agcggacgca gttgttcatt acgcggcaga atcccataac gataattcat
    10441 taaaagatcc tttcccattt gtacaaacga atatcattgg tacgtataca cttttagaag
    10501 cttgccgcaa gtatgatgta cgctatcatc acgtatctac tgacgaggtt tacggcgatc
    10561 ttccattacg tgaagacctg ccaggacatg gggaaggcga aggagaaaaa ttcactgcgg
    10621 aaacaccata taatccttca agtccatatt cttctacaaa agctggttca gatctgttag
    10681 taaaagcatg ggtgcgttct tttggcttac gtgcaaccat ttcaaactgt tcaaacaact
    10741 atggtcctta tcagcatatt gaaaaattta ttcctcgcca agtaacgagt atcttaagcg
    10801 gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg tgactggatc catacgaatg
    10861 atcattcttc agcagtgtgg ctgattttga cgaaaggcag aattggcgaa acctatctga
    10921 tcggtgcaga cggtgaagaa gataataaga ccgtgatgga attgatctta gaaatgatgg
    10981 gccaaccaaa agacgcatac gaccatgtaa acgaccgcgc agggcacgat ttgcgttatg
    11041 caatcgattc gacaaaattg cgagaagagc taggttggca gcctgagttt acgaacttcc
    11101 gtgatgggtt ggctgacacg atcaaatggt acgaagaaca tgaagactgg tggaaaaaag
    11161 aaaaagaggc cgttgaagca gcatatgcca aaaatggaca ataatacaga ggaaatttca
    11221 ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc tgtctctttt ttaaaataaa
    11281 aatcaaggag cggataacca tatgttttta ttaactggag gaaatggaca attaggtaca
    11341 gaattgcgcc atttattaga tgaaaaaaat ctgccatatg tttcaacaga tgcaaaagac
    11401 ttggatatca cagatgctga gaaaacaatg gcttatatta cagaattaaa acccgatgtt
    11461 attttccatt gcgcagctta cacagcagta gataaagcag aagaagaagc aaaagaatta
    11521 gatgagaaaa tcaatgtcga tggcacaaga aatgtagcac ttgcagcaaa agctgtgaat
    11581 gcacaattcg tctatatcag tacagactat gttttcgatg gaaaaaacaa aaatgtagaa
    11641 tacgaagtag acgaccaaac gaatccacta aatgaatatg gtcgtacgaa attattaggc
    11701 gaaaaagcag tagaagagat tttagaagac tattatatca tacgaacttc ttgggttttc
    11761 gggatctatg gacacaattt cgtcttcacg atgcaacgcc tagcggaaac ccgcgacaaa
    11821 ctgactgtag taaatgacca gtttgggcga ccaacttgga cacgtacttt ggcagaattt
    11881 atggtatatg tcatcgaaga aaaagcacca tttggtattt atcatttgtc gaacgaaaat
    11941 agctgttctt ggtatgagtt cgcaaaagaa atattgaaag atacagatgt agaagttgca
    12001 cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc cgcagtattc agtcatgagc
    12061 ctgaaaaaga cagaagcgtt aggttttgtc ataccaactt ggcaagaagc acttgcgcaa
    12121 atgctggaag ctgccaaaaa aatgaataag taatttcctt tttatcaaat agaatttgga
    12181 gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa aagcgaatca
    12241 ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta ttggcgtttt
    12301 agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg tttgggataa
    12361 atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac agaatcggga
    12421 agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag ctgaagagga
    12481 cttttcgttg gtgaacgagg attattcatt aaacggacaa aacatgatca ttgcgtataa
    12541 ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat tctttttcct
    12601 cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca tggtcttgct
    12661 tggattcgaa gtactgatga tattaacgaa gaggaaaaaa gctctatcgc taatcggtgc
    12721 atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg tgggcgattt
    12781 gattttcttt actttgggac tgatgattgc tttttatcat tatttctaca atcatgataa
    12841 aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg ggtttatttt
    12901 agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt attttgttgg
    12961 cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag tgttgattgg
    13021 aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt caaaagacgc
    13081 attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg gaggaaagtg
    13141 gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg aagatatcaa
    13201 ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg tattcctagc
    13261 aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg gacgtatttt
    13321 gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa aatggctagc
    13381 agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt ttagttttgc
    13441 ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg ttattcctat
    13501 ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg cgattacgca
    13561 ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg tgttgggaac
    13621 agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg cttttgtcgc
    13681 cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag gagcgatttt
    13741 tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg gtaaagtttg
    13801 gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat ccttcaatac
    13861 agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag aagaatacga
    13921 gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag atacaaagtt
    13981 cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg cagaaaaatt
    14041 agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat tatatgaagc
    14101 gcagttcacg cagctatatg gaccggataa agacggttat atgattttta aagtagaata
    14161 tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag tcgtacagta
    14221 ataggctagc tgagaataat cctgaaatat tagtagaaag gagagtgaag ataattcaag
    14281 caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg ttgctgacgc
    14341 tagtcttttt tgtttggaca gcaggggtag gttttccagc aactttgttt atatataaca
    14401 atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat gtttataaag
    14461 caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt ttcttttctt
    14521 actttcggga attaagaaat gagtctttat ttggagactt actgtttcct ttggttattt
    14581 tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt atttatctga
    14641 ttgtctttac ggtcttcctc ggaatcacgt tatatcgtgt atttacagag atccaagtac
    14701 cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg atcaatacga
    14761 atacaatcgg aagttctttg atgactcttg ctttattgat cagtggattt gccagttctt
    14821 ttgaacgatg gtatgttcga atcatcggtg ggatagcagt cgttgcagcc tttttaggta
    14881 tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg atattggatc
    14941 ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcctac acggttacga
    15001 ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc aatttgttta
    15061 ccgatcgaga agatatttgg cataaattct atcagacgct aggagaaaaa tctgaacaag
    15121 ttttactagg aatgaaaaca tttatcttcc agcgaggaaa ccaattttta ggaaaccaca
    15181 atagttataa ttctattttg aatatctatg gattgatagg atttgggatt gctgctttgc
    15241 tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac gggcagatga
    15301 cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat acattgactt
    15361 catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat cgatatgatg
    15421 aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat atgacttatg
    15481 atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc caacgctatc
    15541 attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt tttggtttat
    15601 tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg tatcatagaa
    15661 aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat ggcacttgac
    15721 tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt atttatttat
    15781 gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg acttcaaatc
    15841 aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc tatttactgt
    15901 attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg atgcaaaata
    15961 cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct cagatgcatt
    16021 tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag tcgtattcaa
    16081 tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccatt tattcttcat
    16141 tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa tgtctttaca
    16201 gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt ggctgacggc
    16261 atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc tgcagttgat
    16321 catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc ttgtaagcat
    16381 attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat ttttaagtga
    16441 agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca cgttagtaat
    16501 gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag atgtgctgta
    16561 aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa atcttataat
    16621 atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa gaaatcctat
    16681 catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg tgagatgatc
    16741 ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat tacgggagta
    16801 ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt gctagaacta
    16861 ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa tggaatggtt
    16921 ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga atttgctgac
    16981 atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt cgttcgtcta
    17041 gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga agcattggca
    17101 gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat caagaactcg
    17161 ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt ttgtgatcgt
    17221 tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga agtaacgacg
    17281 aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa taaaaaccaa
    17341 gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt gttggataaa
    17401 gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa agtaacttat
    17461 gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg aacagtcgac
    17521 aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc ttggaaaaaa
    17581 ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg tgaatattat
    17641 tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa gacaaagtac
    17701 atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt agaccataaa
    17761 tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag atgaacaaag
    17821 cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag agtttggtcc
    17881 cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag tgagcattgt
    17941 tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg gattttatgg
    18001 tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg attacgtctt
    18061 gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga aagtcagaga
    18121 atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc ataattctgt
    18181 catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc tagatgaaac
    18241 ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag cgggattatt
    18301 cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa aatcaactta
    18361 tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg gtgaagtcta
    18421 ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg cagagccaca
    18481 aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag atgaaacaat
    18541 ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt tccccgtatc
    18601 agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta tcgaattcag
    18661 cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag tcaaaacagt
    18721 aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa cggatatttc
    18781 tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga caccaacaat
    18841 gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc tttctgagaa
    18901 aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa gactagacca
    18961 tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg aatggacaat
    19021 aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag aaaaagaact
    19081 ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa aaatcatcgg
    19141 atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag ctgaccaatc
    19201 gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag aacagctcgc
    19261 aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac gttcattctt
    19321 agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa taatgattaa
    19381 cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt atttatcgat
    19441 caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc aattgatgaa
    19501 gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc tgaagcaaca
    19561 catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac ccaaagcggt
    19621 ttcaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt ccaaacagcg
    19681 agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga tgacggcatt
    19741 gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta tgcaaggact
    19801 catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg atcgatcgac
    19861 tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag gaaagaagca
    19921 caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa cgttgaaatc
    19981 aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca ttgggaacta
    20041 tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga atcctatcaa
    20101 gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat cagtctggca
    20161 actaattctg cattggaaat agcagaagga gaatttatag cactcttaga taatgatgat
    20221 gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca tccagaactg
    20281 gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt tgatccgcat
    20341 ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc tcacttaggc
    20401 gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta tgaaggttct
    20461 caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca tatttaccac
    20521 attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag taatggcgaa
    20581 gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct gtctagaaga
    20641 aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat tgcttatgac
    20701 gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta tgaagatcta
    20761 aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga aatcattatt
    20821 gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata caagcatcag
    20881 ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc tcgaattaac
    20941 aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa cgatacagaa
    21001 gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga tcgaatcggc
    21061 tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg agtattatta
    21121 ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca ttgtggttat
    21181 ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg tatgatggtc
    21241 aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt agcattcaac
    21301 gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta tgcacatcaa
    21361 gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc agaaaaacaa
    21421 agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata tattgcgcat
    21481 gatccatttt acaatgataa tttaacaaaa gacggaatcg gcgacttttc gttgagacca
    21541 gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac ggctttttat
    21601 agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg aggttgggac
    21661 agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt caaatttttg
    21721 tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt cgtttttaaa
    21781 gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct cagagcgcca
    21841 atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa tcgatatttt
    21901 attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca aaagccactc
    21961 tatcaattat catgggttta aaataagaag tgatggagat aaccagattg ttcttagcgt
    22021 caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc tcagaatcta
    22081 atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca ctggttctag
    22141 gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta tacatccctt
    22201 tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac aaaccatatc
    22261 ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga gacttatcca
    22321 ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg tttggtatcc
    22381 ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta cttgtctgat
    22441 acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg ccggttatac
    22501 cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt gaacagactt
    22561 ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag tgcttgattg
    22621 taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa taatctaata
    22681 ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc agcaaagata
    22741 caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa ccattccatt
    22801 catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata tttggttgtc
    22861 gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc actttcttct
    22921 ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg taatacaggt
    22981 attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata taaaggttca
    23041 ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta ccaacattag
    23101 tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt agaggtgtat
    23161 aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac taggttttta
    23221 aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt atatatgaat
    23281 aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga tgttattgta
    23341 tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat cccattacga
    23401 aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat tgcgttgaac
    23461 attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt gtttgtcaca
    23521 gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc gggccgctgg
    23581 tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct tttcttaagt
    23641 atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa aaaggtcgtc
    23701 attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc aaagaaaaac
    23761 aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat caaaaatatg
    23821 attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga aaaaatcaaa
    23881 atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac atttgaaaac
    23941 ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat tgaagcatct
    24001 ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat catcgtttca
    24061 cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt agtcaaagtg
    24121 acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca aaaggaattt
    24181 tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg accagtttta
    24241 gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc tgtccgcttt
    24301 gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt aggaccacgt
    24361 cctgaacgtt ctttcttcgt cgatcagttc aatgcagaaa acccttatta ctacttacgg
    24421 cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata tgtctctgat
    24481 tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt gatgcttgat
    24541 ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc tcaaggactt
    24601 gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga aaaaatcttt
    24661 cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt accagtatac
    24721 aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga ttatgaagag
    24781 tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat cattcaagaa
    24841 ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg gcccggatta
    24901 gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt cgatgcagat
    24961 gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa gagtccaaaa
    25021 gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag aatcgtttcc
    25081 gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt tataaaagac
    25141 atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg ctatcgtagc
    25201 gatattctga aaaataacca aattctattt aaaacttata atagttgtga ggatcgaata
    25261 tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa agtagaatat
    25321 atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa caagttcgaa
    25381 acatttaaag aattctattc attagcaaat actgtaacag acgatattaa taaagacggt
    25441 atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta tagtacctct
    25501 gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga tccagtgatc
    25561 cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac aaagttgctt
    25621 tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt cgttgaagtg
    25681 aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga ggaaaatata
    25741 atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga gtctttcatg
    25801 atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt gatttatgaa
    25861 gattatgcag atgttcaaga ctatcattca gagattgaaa gattaggagg ccgtattt
//
LOCUS       DAPCMT010000025        25685 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPCMT010000025 DAPCMT010000000
VERSION     DAPCMT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.025"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            137..379
                     /locus_tag="RD743_000868"
     CDS             137..379
                     /locus_tag="RD743_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDU0562724.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            397..699
                     /locus_tag="RD743_000869"
     CDS             397..699
                     /locus_tag="RD743_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562725.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            770..2824
                     /locus_tag="RD743_000870"
     CDS             770..2824
                     /locus_tag="RD743_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562726.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            2824..4656
                     /locus_tag="RD743_000871"
     CDS             2824..4656
                     /locus_tag="RD743_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDU0562727.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            5369..7267
                     /gene="ltrA"
                     /locus_tag="RD743_000872"
     CDS             5369..7267
                     /gene="ltrA"
                     /locus_tag="RD743_000872"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDU0562728.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            7384..8184
                     /locus_tag="RD743_000873"
                     /pseudo
     CDS             7384..8184
                     /locus_tag="RD743_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            8181..10712
                     /locus_tag="RD743_000874"
     CDS             8181..10712
                     /locus_tag="RD743_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562729.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            10762..11094
                     /locus_tag="RD743_000875"
     CDS             10762..11094
                     /locus_tag="RD743_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562730.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            11095..11688
                     /locus_tag="RD743_000876"
     CDS             11095..11688
                     /locus_tag="RD743_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562731.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            11851..13545
                     /locus_tag="RD743_000877"
     CDS             11851..13545
                     /locus_tag="RD743_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDU0562732.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            14233..16110
                     /gene="ltrA"
                     /locus_tag="RD743_000878"
     CDS             14233..16110
                     /gene="ltrA"
                     /locus_tag="RD743_000878"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDU0562733.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            16241..16618
                     /locus_tag="RD743_000879"
                     /pseudo
     CDS             16241..16618
                     /locus_tag="RD743_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            16618..17823
                     /locus_tag="RD743_000880"
     CDS             16618..17823
                     /locus_tag="RD743_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDU0562734.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            17839..18483
                     /locus_tag="RD743_000881"
     CDS             17839..18483
                     /locus_tag="RD743_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562735.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            18494..19300
                     /locus_tag="RD743_000882"
     CDS             18494..19300
                     /locus_tag="RD743_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562736.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            19322..19552
                     /locus_tag="RD743_000883"
     CDS             19322..19552
                     /locus_tag="RD743_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562737.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            20098..20373
                     /locus_tag="RD743_000884"
     CDS             20098..20373
                     /locus_tag="RD743_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562738.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            20415..20837
                     /locus_tag="RD743_000885"
     CDS             20415..20837
                     /locus_tag="RD743_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562739.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            20856..22448
                     /locus_tag="RD743_000886"
     CDS             20856..22448
                     /locus_tag="RD743_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562740.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            22460..22795
                     /locus_tag="RD743_000887"
     CDS             22460..22795
                     /locus_tag="RD743_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562741.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            22942..23136
                     /locus_tag="RD743_000888"
     CDS             22942..23136
                     /locus_tag="RD743_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562742.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            23168..25540
                     /locus_tag="RD743_000889"
     CDS             23168..25540
                     /locus_tag="RD743_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562743.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         9731 a         4041 c         5327 g         6586 t
ORIGIN      
        1 ttgttgttcc ctatgagaag gatggcgtaa aaaaaataat ttttcttgga tctagcaagg
       61 atgagaagcg aacgaaagag aaattaattc atgatggatc acaatcaaat aaatacaaat
      121 tacaaaagac tagcgagtga ttgctagtct ttttatttgt cctgaaggag gaatcacagt
      181 ggcaaaagct aaaacgccat ttgaaaaagc cgcgtctaaa atcttagaac gccgcggtgt
      241 atctgaaaca cagtggaaac aagaagtcct tgaaaaagca caatttgacc tattacgggg
      301 aaaagacaag gacttagaag gatatatcta tcaacgtgaa cgtgaaaagc ttgttttcga
      361 tgaaattatt aaacactaaa aggagcgaac aattttatgt ttgaatcaat tacaatgaat
      421 tttttaacac aaccgaaagt agtagctgcg gttgatattt ttagagcatt acaaactgcc
      481 gcaactagtt tgaaaaataa tctgatgatt tttgctgctg ttgcggcagt ggcttccatt
      541 gctcttggag gtattttctt cttctttggg cgaagaggag ctgaaaacgg gaagacacac
      601 gtaggttatg tattgttggg ggtaggagtt attttagctg ccgcttctat tgtagtttca
      661 atgttctcaa tgtttggaca aaccccacaa ggactataaa aaaacaagta gaaggagtaa
      721 aaagtacgtg gctacttttt actccttttt ttatggaagg agaatattca tgttttttac
      781 gatttcaggc aatgcaaaat tggataaaga gttaataaga aaagcaacga agaaagtggg
      841 aaagcgtact tcctttttac cgaaaacaat taaatattct gtcattgaaa cggaagaaca
      901 gctacatgtg ttaggacaag tgttagaaca agagaatcaa tcttctgtta aattgactct
      961 cacgttacat gataaagaag gaaaaataga aaaggctggc acagaatacg tttttggctc
     1021 actggattat caaatttcag aaggttttta tcctacattg attgatttaa tccgcctaga
     1081 tatatttaaa gatacggatg aaaccatgag tgtggaagag tcttatcgaa acgaaaaaat
     1141 tttacaagtt ttagaagaag agttagaaag cgaccaaaga aaagtgaatc aagtgagtga
     1201 agtcgaagca aacgaagaag taccatggga acgcccaatc tttccaaagg atgaatcaaa
     1261 agaaaaagag aaaggaatta ttgacgaaca agctgacatt ttgactcata aatcaatatt
     1321 tgaggaagag aacgaggtaa tgggtcaatt ggaggtagat ccctttgaag aaacaatgga
     1381 ggatgaagag acggccgaag tagaagttga tccagttgaa gctaatgaag acccaacatg
     1441 gactgaaggg gattctcagg aatatattga accacttatc agtgaagaag tagagaaaaa
     1501 tgaaacagag atacaaaagg caacggtatt tgattatatt ttggatcaat atccgccaga
     1561 gcatgaatgg atgaaagaaa aaaataaatt atttttaaat gaactttatt cgaaatatca
     1621 attacctcaa acctatcaag catttttaga gagtaaagaa gaattaatct cacagggtaa
     1681 aggcacactt ttagaacagt tagagtctgt ccaatcaaca aattggcatg agcttgcaca
     1741 agaagattta ttagctactt ttgaagaacg tcaacaagaa acagatcaag aaattaatga
     1801 gtatgcgacg gaacagtcgt cgaattggga gaaagcaaaa gaggaactaa ataaagaaga
     1861 acagcgagtg attgaagaag aagtacaaaa aattaaagag cggtacgaaa agaaacgtga
     1921 caatgcgtat tccttgtgtg tagaaaagat caacaatttt actcagacga atcaagaagc
     1981 attatcgaaa gaaaaagaaa acttattaaa agaacgtata aaagaacgaa aagaagaatt
     2041 ttaccgaggg ttgcgtactg ataagctaag gattagcaaa gaattaaatg aacaactgaa
     2101 taaactctat caaactacga ctgaagttct gatggaaaaa cacgagcaga tccaacaaga
     2161 attacaagca caaattccaa aatggaaaaa agagtatgaa attcaccaac aagaattgaa
     2221 agaacaagaa gaaaaacaac ggaaaaaaga aaaagagcgc gaacaattag aactaaaaaa
     2281 acgtgaacag gatcttgcgg aacaaaaaat ggcgatagaa aaacaacggc tagaacaaga
     2341 aaaggaaaaa gaagcagctg ctcgtaagga acgtcaagaa caaatggata cattgcgaca
     2401 agcacagtta aattatctga cacatcctca accagtagct gctgttcaaa cgccagtgaa
     2461 caaccaagca aatttaactc cttcacccca gcgttccttt aacggatgga tggtgggatg
     2521 tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta atcatatcaa
     2581 tcaatcttca caggctgatt ctgtagccac aacggcgtca atcaaggaag aagcccagga
     2641 acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta ccaatcagtc
     2701 aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg ctaaagaagg
     2761 atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
     2821 ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt agaaaagaat
     2881 aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc agaaaaacat
     2941 gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc tttagagtat
     3001 ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg aagcttacaa
     3061 ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga ttcagctggt
     3121 ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat ttcgacagtt
     3181 tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tcctctagca
     3241 gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca gatttatccg
     3301 caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat cacacattac
     3361 aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg tacctctcga
     3421 tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg tgcagaaaaa
     3481 caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag taaagatgaa
     3541 ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact gcaatcaatg
     3601 agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat agatactgca
     3661 acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc ttcagataac
     3721 aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc gttgttagaa
     3781 caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt tgagcttctt
     3841 tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa acagttgaat
     3901 gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa acaatatggg
     3961 gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc aatcacggga
     4021 ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac gattgattat
     4081 aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct tttaaatcaa
     4141 cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg tgtcaaagta
     4201 ggctataaag gatttagtga aatcaatttt gattgtcatt tagttgcagg tgatcaagtt
     4261 tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt ttctcaaaga
     4321 gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga aacccatttg
     4381 ccagaattag aaaaagaagc cgttctaaca ttagatgagg aaaaaagtaa cttattagta
     4441 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct tacacctgat
     4501 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata taaagaatta
     4561 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca ttttatttta
     4621 gatgaatttg ggaatagtgt gacctgttta gcctaaggat aaaagaggct agttccaacg
     4681 aacattggaa taactttact atcgagaaat caaatgacgg ggtaacgccc tgaagggttt
     4741 ccactgatac tgcgacatgc gttagtagga tgtgcatcca aacgcgtatg aagctcgctg
     4801 aagtcggcag aagggtaccg taaggaaaga cacgatacgt atctaccacg caagtgctct
     4861 agaggtaggg tatgtacctg atatgtcggg tgtcctaaaa agttcaataa ggtgagaatg
     4921 atttgattat ctgacaaact tgcgaatgga atgtctgaaa cgaataactc atagaaatgt
     4981 gagggggaag ggggatacgt cctctttcca ttgactcgag gttgagtaaa aatgtgtttc
     5041 tatgaaaaac cttgcatggt taaaggctat ggagtagtca acaggcaaaa gcaagcacct
     5101 aagttgaaat ctatggatag atagtaagga acaggggaag catctagcac ggagggagca
     5161 taccgaagat gtgtttctaa ggaaaaaccg aaatcggtta taacttaggt gaatagatgt
     5221 gaaagtagag ccacgaccag tgaaaatttc gtaatgaaat tggaggaaca ggctcaagtc
     5281 gttatttata atataagaaa cctatttaaa atcatggatt ctcgtaagac taaaaaggtc
     5341 acgaaccaga atggagcgtg ataaaaccat gactaacaca aaaggaatta gcttaaagaa
     5401 caaacaactc agacacgccg aatactacga catgactgag actttcgatg aattgcacgc
     5461 aatgagtgtt gcaaaatcaa attttaaaaa tctcatgaaa ttcattattg atgacaataa
     5521 tatctttcta gcgtatagaa atatcaaaaa gaataaagga agtgcaacgg ccggatacga
     5581 tggtgtcact ataaaagaca ttgaaaaatt ggcatgtggt gactttttaa ctattattaa
     5641 aaggcgattt aaggattaca gaccaagaaa agttaaaaga gtagagatcc cgaaaccaaa
     5701 cggaaaactt cgacctttag gaattccttc aatatgggac aggatagtcc agcaatgtat
     5761 tctacaaatt ttagaaccaa tatgcgaagc aaaatttaat aagcatagct atggattcag
     5821 accaaattgt tctacagaaa acgccatagc agattgtctt gctagaatca atcaaagtaa
     5881 gatgaattat gttgtagata ttgatataaa aggattcttt gacgaagtaa accataccaa
     5941 acttatgcgt caaatatgga caatggggat tcgagataaa caactccttg taattattcg
     6001 taagatgtta aaggctccga ttgttatgcg aaatggcaat gttgtttttc caaccaaggg
     6061 tactccacaa ggagggattt taagtccact gcttgcgaat atatgcctga atgaatttga
     6121 ttggtggata gctaatcaat gggaggaacg aactgtcaaa gagctgaaac accactatcg
     6181 aaaaaacgga gcatgggata aaggaaatat ctattcgaaa ttaagaagcc acacaagttt
     6241 aaaagagatg tatctggtac gctatgcaga tgattttaaa attttctgcc gaacaggaga
     6301 agaagcccaa agggcttttt atgccacaca aaaatggctc agagaaagat taaagctccc
     6361 aatttcaatg gagaaatcta aagttacgaa cctaaataaa gctgaaagtg aattcttggg
     6421 tttttcaata agactccaca aaaagggaaa aaagaaagtg gctttcacgt acgtttgtcg
     6481 caaagcagaa gaaaacatta aaaataaact aaaacaacag attaaaaaaa ttcaatacgc
     6541 tggagcaaaa aaaaataggc accgagcaat aggtgactac aacatgatgg tcatcggtat
     6601 ccacaattac tatcggatag cttcacattg tagtgtcaat ttcactgaga tttatagaag
     6661 tgttaggaca gtaatagaag caagactgaa agctagagga atgacgaaaa atggaatcta
     6721 tggacaagat catgaaggat accgtccgta tctaaagtct aagcaaatga gatatattga
     6781 aggttttccg atacttccaa ttggctatat taaaaaaaga actccactca acaagcggag
     6841 aaatctaaat aaatatacca aggaagggag aaagctcatt catcaagagc aacaagctat
     6901 agaaagctgg aaaatcaagt atcttgtaaa aggttttagt aacaataaga aatacacggt
     6961 ggaattttct gataacaaaa tatctaagtt tatttcccaa aaagggaaat gcgctatcac
     7021 aggaaacgaa cttttgatgg aagaaattga atgccatcac attaacccat atcatttgtc
     7081 caaagacgat agttacaaga accttgtcat tatctctaga gaaatccata gattaatcca
     7141 tgcaactagt gaagaaacta ttctgaaact gcttcggtta ctgaaactaa atcactatca
     7201 aattagaaaa ctaaacacgt atcgaaaaaa agcagctaat tttccaatat caatcaaaat
     7261 gaattagata ggaaacaaat tatattgtaa ttaacgatgg aacgccgtgt gcggtgaaag
     7321 tcgcacgcac ggtgtggacc gggggaaaag acggagataa tatcaaactc ttacctatcg
     7381 gaattgccac caatggataa tatggaagga atcatgacgg tcacagctgg ccgaaacatg
     7441 ttgtgggatc tgtttattca atcttatcag caactttatg cgaagtatgg agaacaaaac
     7501 gcagcgatta tcaaagaaaa ttgtcagaca catatttata ttatgtcgac agatgataat
     7561 accatcgaag aattttctaa aaaagtgggg aataagacgg ttgaacaaga gaacagtacc
     7621 atcaattcat taggtatgaa tactcatatc aatcgcaatg ttgattcaga tcgtatctta
     7681 actcctgaaa gaatttctac ttttttagaa ggtgaaacga ttgtactcgc tcctttaaaa
     7741 cgacaagatt tgaaaggtca taaaatacgg ccgtatccta tttttaatca tggggaaacg
     7801 aatcttcctt atgcttatca atatattgaa gatttcaatc catcgtggga tatcaatgat
     7861 atagacatac attgtacaca tgcggacttg gatcttaagg ctttacaaat tgattatgat
     7921 tatttcctac cagatgaata tgctagaaat ctctatcatg gactgcatga tcaattagaa
     7981 gaaaaaaacg aagaaaatat gttttataaa atcgttcaat ctcttgagaa tgagacggct
     8041 aaaaaattgt taattcaatt ttacgaagaa caagcagcag atcgacttca agaatatgtt
     8101 gaaaagttga tcaaaaatga agaaatttct gaaatgattg ggaaacggtt gttattggaa
     8161 tctgaaaagg tggtgaaaga atgatcattg gaggaatttt ttctggcata gccgactggt
     8221 ggtcaggctt attcggagaa ggggatgcaa accccaaaac cgtaacgaaa gtcttacaag
     8281 attatggtaa ttatctgcag tatcaagatg tgttcagttg gatttgtaat acttttttat
     8341 gggggttgat caaagggtta tataatctca atactttact agaaaaaacc atttatcaaa
     8401 gctttgattt aaaagactta ttgaacgccg ctggggtaaa tgaactattt caaagtttga
     8461 ttagtaaggt attggctctt ttttgtgtcg tcacattaat ttatttaggc ttgaaatttt
     8521 ttatgagtaa acacccacca aaagtcaaaa atatcttggt taatttattt atggccatga
     8581 tcttgatttt tggtggtagt tctttaatcg atgaagggtt aaatatcagt aaaagctttt
     8641 acggagacgt cactgcagca aataaagcag ataaaacggg ttcacctgct tttcaaatta
     8701 ttcaaaataa tgtgatcgat gtcagtacat tactagcaac tgacccgaat aaagtagaat
     8761 ccctgccaac agacaaaaga aatgctttaa cagcgaaaaa ttttaaaaca gcgaatatca
     8821 atgggattat tactcctaaa caagctaaag aaatggcgga taaagataag agtggtttat
     8881 cagatttaca aaaggatcgg ttaagagcgt tacaataccg actggaactg gatgataatg
     8941 gaaaagaaat tcctgtgcaa atcagtgatg aaggcttagc caaatatgtt tatacttcag
     9001 ggtatcgtcg atacattagt agtccaggga ttattttagc tggtgaatct tctttggcgg
     9061 tggcctattt atttatccta tttacgatcg ttacttgcat tattgagctg gtatttaaaa
     9121 aattctattt agtgattgcc gcgtcaacgg actttgagac aggacaacgg atgaaaagtg
     9181 ctatcgaaga tatttctcaa tccttcttgt tattggcatt tacaatttta gaattacgaa
     9241 tctatacctt aatgctttca ggtattggtg atttgcatgc ggcaggaaag atcaatggat
     9301 tcttgtatgt tgtcgctttg attgtactaa ccattgcgct attcaaaggc tctcaatcag
     9361 ttacgaaaat tttcggcgtt gatacgagtc tgaaaaacgg cagtaattct cttcttagca
     9421 tgtttgcctt aggaaatatt gcgaaaaatg tgggtagcgg agctaaaaac cttgcaagtg
     9481 ctggtaagag tggtttaagc catttaaatg atattcgtaa aaatggattt ggccatcgaa
     9541 aagatgttac ccctgataac ggggaagaac aatcagacga taaagctaca actgtaccta
     9601 atagtaagaa aacagtttcc gaaatgttcg ggaaagcgaa aaatggtgtt tctaaagccg
     9661 cagaaggtct tggttacgtc aatgaacgtg ggttaaaagg aacagctgaa gatcttacag
     9721 aaaaagccaa agacacctta gaagataaat tagacggcac gagagctaaa gaattggctg
     9781 aagctgtgaa gaatccatcc gaagcactag aaaaacaaaa agaaaaagct tcagaagtca
     9841 aagaaggact aaaagatgcg tatggtgaag gacaagtcaa tgcagcagtg aaagctaata
     9901 caacaaccga aaaagaaaat caagaaggta tagtgattcc ttcagtcaaa gatcaccaag
     9961 aagcgttgca ctctaatgaa aattcgattg aagagaaaaa ttccatagaa gaaagaaatt
    10021 cagaagaagg aaaaaatgga cagttaccaa aaactgatcc agaacaatat cgctataaac
    10081 ctaacagtga ccttgaaaat gagaagcgaa tttcagattt atcaaaagta aatcaagaag
    10141 acaaagcaga aaatgaggga cagttaccaa aaactgatcc agaacaatat cggtatagat
    10201 ctgttaactc cgaaaatgaa aaacgcattt cagatttacc aaaacgaaat caagaagtac
    10261 aaacggatga ggattccatc gttctgccaa atgataatgg aatcgaagtt ggaactgaaa
    10321 aatcaacgaa aattggttta cctaaggcac aaaatttacc aatgaaagat agtcgtttga
    10381 atcaattcaa ggataatgat caaagttttg ttcatcaatc acctgcaggt actgttcaaa
    10441 tgaaaccaag tcgtttgcct gaaaatggaa atctgacaac tgtttcttac aatttatctg
    10501 aaagtggtga gacagggaat gcgtccgttc aaaatgttgt gacaagagaa gctgcaccaa
    10561 aacctattca aaacgttcat acaggttaca agccagcaaa tgtggcattt actgcaccaa
    10621 caaaaataaa tgtgacaaga gaaactaaaa acaggaacaa tgcattgaaa gaacaactat
    10681 tgagggatat caaacaggat atcaaaccat aaaaggccta caagttaggc cttttcttta
    10741 tatgaaaagg agaaatcaat tatggataaa tatataaaca tgaaaaaccc tccaagattt
    10801 agtggagaat ggaagttttt cgcttttttc tttttagatc tattaacgct tggtggctgc
    10861 tttttttggg cgaaaatggt caataatctt gtccacttac cactcttttt tgcactcttg
    10921 aattatggct tatggattgc gatagcgtta gtgattgttt ggcgacccta taataaccca
    10981 ggtcaacgac aaatttttgc gattatcgag tctatttttt ttgcggacaa gaaccattat
    11041 catgcgttag atcctaaccg aaattatcat ggtacgtata aggaggatga ataaatgcga
    11101 gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc agtttggaaa accagaagag
    11161 cgctctatct tggacttatt tccgtataaa tcctgtacag aagagggagt cttaattact
    11221 agagaagatc attttcaacg ttattatcgt gtagtttcta cagacgtgga aggattaaat
    11281 gaacaagaga aactagaacg aatgaatcag ttgaccaccg tcatgcgtac gtatgtgcca
    11341 acaattaagt tagttagttt gaccacagag acggatctaa cggaacaaat cgtgcaaaag
    11401 cggcagttat tagataaaaa tcgtatggaa cagtccttag gaagaaatct acagcgatta
    11461 agaaaatatg aaaagaaatt agtagaggaa atccaggaat tgaaacgagc agaacaggaa
    11521 cgccctgatt taagcttctt tttcgtcatt gaaggaaaga caatcaagga tttgcaaacc
    11581 agaaaccgtc agttaatgcg atctagtggc attttagggt taaaaccact ctacaaaaaa
    11641 gaattgatca agattcttta tcgaatgaat aatatgaatg atgagtagga atggggaaga
    11701 gagaatgaaa tatccgttga tatggttaga aaaagatgtt ccgaaagaag agaccactag
    11761 aaaatttcct cacactttaa gcctgggtaa gttgggaaaa ggaaaatctt ttaaccaagt
    11821 aaagaatcaa acaagaagag gtgactgtac atgttagaag caattaaaac agctttttca
    11881 ccacaaaatg atcaaaaggt acttgatcca ttaaagcaac taggaaatcg ggaaatagaa
    11941 attttaaaat ccaaagggta tgatttagac tttttaaaac aagtgatgcc acaaggtgga
    12001 ttaaatttcg atgaagagta tgtgacctat ggaaatggtt attcacgatg tgtgacagtt
    12061 tatcactaca caaaaaatcc tacgcttttt tggttggcca atctcatgaa caatcctcat
    12121 acagtggcaa cactagatgt tagcacagat gaaaagcaaa aagtgattga tcaaatcaat
    12181 cgtgccttag atgaattaaa tgatcggtca gataatgaac gaaaagcgac tgaccgaaat
    12241 aaaagcgcgg aagaatacct agaactagcg gaatatgccc gaaaattgac ttctaatggg
    12301 gaaatttcta aacaagtcaa agtgagagta catgtttacg gaaacacgat tcaagaagta
    12361 gatgaacgag cacaggaact gatcgaaaat ctcaaaggga tggattatcg tgcagtgacc
    12421 tatctgcaca aaacgaaaag agactggcaa tcgatgttca ccatgttcga tgaacaagct
    12481 gaatggtttg gaaatatcaa agggcaatca ctaccatcgc taaacattgg cggaggtttc
    12541 ccgttccacc accaaacgct aaaagatcca ggtggaacac aatatgggga aaccatgaca
    12601 ggcggcccgt ttgtttggaa tgccacgtat aaaaatagtg tgcggttgtc taccaatacc
    12661 ttgcttctag gaatgatggg agctggtaaa tcgaccgttc ttaaaatgat cgcggaagcc
    12721 catttagctg caggagactt tatttggggc tttgaaaagg gaaaagactt tattcccttt
    12781 ttaaaggaat ataacggcat tatggtgcgt ttagacggat ctgacgggat gatcaatcct
    12841 ttagaaatat ttgccacacg tacgtatgat gaagcttcca gtttatatga cgatggatca
    12901 gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa cgcacttaga caaagtggtc
    12961 tatcaggtac agttagtgag tcctcagcta aaaggaacga tgaaagcaga atttaagacc
    13021 tatttaaatc gtttttatga agagtatgga acagtaccac gcggctttac ttcctcaaat
    13081 tcgcgttcaa atactgatac gcaagtcaca ggtaaagatc cagaagctta tccgacgttc
    13141 aaagaatttt tagacttttt aggacaactg gaattaccag gtgcttccca agagaaaaag
    13201 aatcgaaaag aagaaatgga atccatcgta gaatccttat gtgagacgta tggcatgatt
    13261 tttgatggtc atagtacgat tcgacacctt gaccaacaac agttggtctt ttttgacatt
    13321 gactctatta caggcttaga tcgaggggta caacaatgtc aaatgtacat ggctatgacg
    13381 ttgatttgga atcaggcatt gattcaagga agaagacaac gacgtttaat taaagaaaaa
    13441 caaatggaag aacaagactt caaatgcttc ttggcgatga ttgatgagtg ccataatggt
    13501 gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag tctaaaaact attgtaataa
    13561 ctcttataac gagaagtcaa atgagtgggt aacgccacaa agggcttcca ctgatactcc
    13621 gacatgcaac aagtagttta tgcagaccaa cgttgtgtga agctcggtga agtcggctga
    13681 aggataccgt aaggtaaaag aaatagtgaa taccacgaaa gttctctaga ggtaaagggt
    13741 gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt gagtatgtac aaacgtactg
    13801 acgaactagc gaatgtactg tccgaaaggc atagctccta gaaatgggag agacagtgag
    13861 caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa gtcgttgtta tgaaagacca
    13921 tgctgcgtta caggcgtagg agaagttagg gggcaaaagc tagcatctaa gttaaggcat
    13981 gtggatagat ataagggaac aggggaagcc atcaacatgg agagtgcata ctcgatgaag
    14041 tggtagtaag aaatatgtga atgacaaata acttacttta gtgatggtga aagtagagtc
    14101 acgactgatg aaggttctgt aatggaactg gaggaacagg ctcgattcgt ctgtaaaagt
    14161 catgaaaccc gttcaaccat gggattctcg tatgactaaa aaggtcacga gccaattgga
    14221 gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata ggaagttgcg gcatgcagaa
    14281 tattatggca tgacggaaat cttcgataag atatactcca aaagtttgaa gggagataat
    14341 ttcaagaatc taatgcagat tatcatttct gataacaaca ttgtcctagc atatcgtaat
    14401 atcaagagaa atcacggtag tgtgacaaga ggagaagatg gagtaacgat taaagatatt
    14461 gaaaatctca accgtaatga attcgtagaa attgttagga agcgattcgc acattataac
    14521 cctcggaaag taaggcgtgt agaaattcct aaatataatg gtaaattaag accgttgggt
    14581 attccatcaa tatgggatag aatcgcgcag caatgcatct tacaagtatt agaaccaata
    14641 tgtgaagcaa aattcaataa tcacagctat ggtttccgcc caaatagatc agcagaacac
    14701 gcaatagcag attgtatggc ccgaatgaat caaagtcaca tggaatatgt ggtagatgta
    14761 gatatcaaag gtttcttcga cgaagtcaat catactaaac tcatgagaca aatctggtct
    14821 atgggtatac gagacaaaca gcttctctta atcattagga aaatgttaag agcacccgta
    14881 gtacttccaa atgggaagtt gatataccca acaaaaggaa caccacaagg cggaatactt
    14941 agcccattac ttgcgaacat caatctcaac gaatttgact ggtggatagc aaatcagtgg
    15001 gaggaaaaag atgccaaaga gctaaaacat cattttagga aaaatggagc ttgggataaa
    15061 ggtaatatct atcacaagct cagaaagagt acaactctaa aagaaatata cctagtgaga
    15121 tacgcagatg attttaaaat tttctgtaga aattataatg aagcgaaaaa agttttctat
    15181 gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa tatctgaaga aaagtcaaaa
    15241 atcacaaatc tttcaaaaga agaaagtgaa tttttaggct ttacgatgaa attagtgacc
    15301 aaaggtaaca aaaaggttgt taatacgcat ataggtacaa aagcacaaag catgattaaa
    15361 gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc ctaatagcaa agataccttt
    15421 atctatattg gagtctataa cagtatggtt attgggattc ataattactt caaaattgcg
    15481 actcattgta gtgtagcaat gcgaaaaatt gcaagagaac tcagaccaag ttttgaaata
    15541 cgactacgag aacaaggaat ctcaaaagaa ggacaatacg gaaagtacga aggatacaag
    15601 ccttacctac ggtcaggaca aatacgatat ataagagggt ttccaatatt accagtaggg
    15661 tatattcaac acagacatcc gatgtgtaaa aaaagaaata ttaataaata caccgaagaa
    15721 ggtagggaag ccattcataa gaagcaacaa accgtagaag aatggaagat atcgtggttg
    15781 tgtagcaatc cagtgcttag taagagagca acgattgaat ttgcggacaa tcgaatttca
    15841 aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg aggagcttat cttatctgaa
    15901 atgcattgtc atcatattat cccttatcat gaaagcaaaa gtgatagcta tgaaaaccta
    15961 gtgattgtta ctgaggaggt tcatcgggta atacacgcta cacaaagtga aacgattgag
    16021 gaacttctaa aatatctaaa attaaatcca aaacaaaaag aaaaactcaa tgagttgaga
    16081 ttaaaagttg gtaacgaaga aatatcttaa taaatatttg taggacggga aaacagaatg
    16141 tcttttatag acgagggaac gccgtatgcg gtgaaagtcg cacgtacggt gtggactggg
    16201 ggaaaagacg gagataatat caaactctta cctatcagta tcgattaatc cagattttat
    16261 tgaaacggta aattatgtca caaactttca acgagaaatg cgtaaagtag aagcaatgac
    16321 tgttttagct acacaatccc cacaagaaat ggtgccagaa aatatgtctt ctgataactt
    16381 ctcgaaactg aagaaagtct ttgaattttc ttctacgaag atctttatgc gtatggatga
    16441 atctatttta cgtcatatag aaggattagt tggtaaatca atgacaaact cagaattaga
    16501 atccattcct caacaaaatc aaggagacgc tgtgatcaat tttggctcaa aagaaaccat
    16561 tcgtgttcat tttagtccga acaaacgtca attaaaattg tttgatggag gaaaataatg
    16621 gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc gtcgtgcaaa gcgacgctta
    16681 tttttgtggt tgtttggtac ttctgcaggt ttgattacta ttttgattac tgtgttcgtg
    16741 accctttttc taatacttgc ggcgggttcg attgataatt ctgattcaga ttcaagtagt
    16801 ggtggcgaag cttttacagg agaatattcg gaaggcttac cgatttataa agaaatcaaa
    16861 ggaagaggcc ctttttctga tgagattgcg caatatgctg taggtgcagc agtgaaatat
    16921 aaattattac caagtgtgat cctttcgcaa tacggttatg aatcagcgtt tggtacgtcg
    16981 gcgtccgcaa gaaatgatct caactatttt gggattactt ggtttgatgg ttgtctgttt
    17041 cctaaaggaa ctgcgcgagg aatcggtggt attgaaggtg gctggtacat gaagtttccc
    17101 aactcaaaag cagccttctc ctattacggc tttatggtag ccacacaatc caattttaat
    17161 gcatgtgtag gaaataaaag ccctggcgca agcttattga tcttaggacg aggtggttat
    17221 gccgctgcag gaattactga agatagtccg tattatacag gttgtatgtc cattatcact
    17281 tctaataaat taaccgaata tgatgaattc gccatcaaac attggggtga aggtggcaat
    17341 aacaacggca cgatcactgg tgaatggacg aatccattcc ctggcagcag tttagataaa
    17401 agttccttca gtggtggtca attatttgga acaaatccag gcggtgaatt ccgtccgaat
    17461 ggcttccatg atggattaga ctttggttca gtcgatcatc caggaagtga aattcacgcg
    17521 gtgcatggag gaaaagtcgt gtacgttggc aatcctggta tttcaggttt aggtgcatgt
    17581 gtgatcgtga ttaattacga cggtttgaat atggtatatc aagaatttgc gaatagtaca
    17641 ggaaattctc gtgtgaaggt tggcgatcaa gtgaaagttg gtcaagtaat cgggatacga
    17701 gatacagcac acttgcattt aggtttcact cgaatggatt ggcgtcaagc acaaggacac
    17761 gcattcacag acgatggtac atggattgat ccattaccat ctttgaatag cagcaaaaaa
    17821 tagatcagga gaaaaaatat ggaaaataaa actttaaaaa tcgtcttaac agctgcaatt
    17881 agcgttagtt taatcggcaa tatcgcgttg ttttggcagc tgaaagaaga acaagagaca
    17941 aataccttta aaattgaaga agtggctaaa aacgcgtctg agaagctcaa aaaaaattcc
    18001 tctgaggcac aggcgaagat cgacaagtta aatcaaacca tcaatgagaa aaaccaagaa
    18061 atcgatcagc tgaagcaaca agatggcaca aaagcaaaag aggaagtaac tgaagcacaa
    18121 agaaagacgg cagaagagtt cggtcaattg gccattgata aatcattaag tagaaatgaa
    18181 ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga tcgacaaatt aagtcctgca
    18241 gacgatgatc accaacatga tgattacgga agttactcct ttacattagg tgacgtgcaa
    18301 acttatgctc aaacaagcag tgtaaaagag gaacaaaatt ttgtcgtatt tgttgattat
    18361 acaatcggca atccgcaatt caaagatgtc aaaccacaaa aaataaaagg tggattaact
    18421 gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag actttagtta tttcactcgt
    18481 taggaggaag aaaatgagta agaaaaaagg agaaagacaa cgtttcaaaa aacgaaaaag
    18541 ttatcttcaa tggctgggaa tctttatgat tctcggcttt ttgattcttg ctagtccgat
    18601 ttatacgaat cagaaggtag atataccaga tgaggtaagc acgaacgcct ttttaaacac
    18661 gaaagagctg acaatggttt caaaagaaaa aggagcgaac cagcaattag tttttgaatt
    18721 tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg gctaatctta attatcgcgt
    18781 agaagtaaag acagctaaag gggattatca agcaatacga gccaaagtga tcaaggtgtc
    18841 tgatgattat tttgcagtga agctaacgca tgtaccagaa gaatatatcg ccatgcgttt
    18901 aaccattatt cctgaaaaaa ttgatccaaa agtggatatg caagaaccac aagatctgat
    18961 ttattacgtg cacgaagata aggttaaaga tcaagtgaaa gatgaagatt atgaacaaca
    19021 cgcgatcaat tacaaagtga aaggatacaa gaaagaacaa aaagcagctc aaaaacaaat
    19081 ggaatctttc caagcaacca ttgatttgaa tgaagaatta gtgaagaagc taaaagatca
    19141 attgccttat caagtcgcag atgatcagga aaatactgaa aacaagatta atagctacca
    19201 gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa gaagagatca ttcaaaaact
    19261 tcaagaaaaa attgatcgta tgacaaaaga aaagatttag aaggacaggt ggagtgaaaa
    19321 aatgaagcga acaaacttgg ctgacaaaga tactttcgct gagcagaaag tattgattaa
    19381 agcctatctc aaagttgtaa aagctaaaga aatatgtcga gaagtgaatt tatctaacac
    19441 aacaatatct gagttgaaga atggtcgaaa gaatttagat actttaaaat tcagatcatt
    19501 tgaagctcta tatgtagctg ctaaaaaata ttttacagct gataacaaat aaaaaacagg
    19561 agtattttat gtgaaaaaag tatataaaaa atgcttatga atcagctata tcaccttaac
    19621 catttgttaa tgagatggga gaaaacatta accacgattc taacgcaaac atcaagcaca
    19681 taaaacattt aacactcctc ctttctggct gaagatttca accaaacagg aagacagcgt
    19741 atcaattata gcatacactt tttcttaaat tcatttttca tatgacaaaa tatttccttc
    19801 taatcgacaa agttagaagg attttttatt gtctaaatac acaaataacc ttaattttta
    19861 aagagcataa ggaaacgtga tatcactttc tcacgaaagg tgttaggtag gctgtgccta
    19921 ccacgtttct aagaaagata aatgatatca aaattttgga tttgtgtgta gatttgtggg
    19981 taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt gtgtagcaaa atcaaaaaat
    20041 gtgtagggat atgtgtagct acacatatcc ctacacataa taaatttgga ggaaaatatg
    20101 gacttattaa ttagaaatat tgaagaaaag tatattaata aaattgacaa acgttgtgaa
    20161 gaacttacgg tcaagactgg caaaaaatgg agtcgaaatc aatacctaaa agttttgatc
    20221 gaaaatgatt ttgatcacgc tcttctgaat tataaaaaag accaatttga tcggttgtta
    20281 gaaaaatttg tggatattca aacttataat acgaaaactt tagaagaata tattgcaaca
    20341 aataatcaat taattggttt gttaattgaa taataaaaat agtattgtga atttataaaa
    20401 ggaggaaatc aattttggca aaagcaacaa gaaaacatgt atatctttac gatatgcata
    20461 tcgaagtact tgatgaaata atttctactc atgaagatat atctaattat agtgaagcag
    20521 tcagacattt gtgtatgatg tatgatccaa aaatcgaaga aaaaataaat ccaattcaaa
    20581 ttaaattgaa tgcaatggga aaagaaattt ctattttaac cgaaatggtt tcagagtttg
    20641 cggatcttca tttgaaagat acaggaatac taattggtac aaattcaact gtttatcaag
    20701 aagcagaaga acgtgtggaa ggaaaaatta aatccaatca aaccaaaaaa tattcttcta
    20761 agttaaagaa accagtgaat gaatctgttc ataccagagt agatccattt gatccaacga
    20821 agattctaaa aagataaaag tagaaggaga acgtgatgaa taaatcgcct gcagttattc
    20881 ttacttgtca atttaccttg cccaataaaa aatctttttc aacatacatt gattatatga
    20941 caagagaaaa agcactggaa gaaatggaac gacgtacgcc cgaagaagaa caggaactgc
    21001 aacgaatcaa gaatgcttta gaggattttt atattcctga aggagaaact ttttcggaaa
    21061 gtaagacgga taaagaattc agtgaaacgt cgaatgaagc tcaatcgttg atggaacatg
    21121 gaatcaattt tgacaagtta gaggaacaag attttaccaa atatctttct tatatgaccc
    21181 gtcactatgc gttagaacaa aagaaagaac tgacgcaaag ggaacaaaaa gaataccaac
    21241 ggttgaacca agcaatgaaa aaatatcaaa cagagccaca taaaaaagct tcaggaaatc
    21301 ttcctggagt tttttctatg gcttcagacg aagtgaaagg aagtgatctg aaggagatca
    21361 aacaaatctt ccaaagagga caacaaaaag gctcaatcat ttatcaagat gttatctctc
    21421 acgacaattc gtatcttgaa aaaatgggct tgtacgatcc caaaacagat accttagatg
    21481 aagaaggttt gaaagcagct ggaagaaaaa tgatggagac attattcgag aaagaacaat
    21541 tgaatgaaac aggttactgg atggcaacga ttcaccgtaa cactaagcat atccatattc
    21601 attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta tactgagatt gaaaatggtg
    21661 tgagttatat cgtaccgaag gggaagagaa gacaagcaac attagacgca atgaaaacca
    21721 attatgtaca tgagttagaa cgtttctctt atgaaaagga tcgaatgatc ggtatggaga
    21781 aacgaaattt attgacacga aaaagtgatt tgcgtaacac cttaacaaaa gtagtaaaag
    21841 aaccagcacg ttacgatcga cacgccatga acttattaaa tgaagtatat aaaaccctac
    21901 ctgaaaaacg ttcggaatgg aattatggag atgaaaatcg aaccaagctt tcaccattta
    21961 caaggggaaa acttgatgag ttaaccaaat atgttttaac aaaagaacct ggatatcaag
    22021 aatatataca gatttcaaaa gcattgaaag aagaagccaa aagcctttat ggggaaagca
    22081 aaagagaaac aaagaatgca gaaaaaaacg ccctctttga tttaaaaaag cgcacaggaa
    22141 atgcgatttt aacagaactg aagagacagg atagtcaaat aaaaccatta atagaaagtg
    22201 tcaatcagat gcaatctatt tacaatgaac gatcctttcg aacactcaaa cagttgccaa
    22261 caaatgaata caagtatagt tatcaagaat tcgtaaagcg taggaaacaa ttccaacaaa
    22321 gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac aattaatgca catgaagaaa
    22381 aataccacgc aagtaaagca tatgaagagc aacagcgaag aatccaaatg gaacaagaaa
    22441 gacaatagga ggaagagaaa tgaaacaata tttagatcaa tggaaagtca tagaaggatc
    22501 actaaaagaa gaacgaatcg aacaacttcc tgattgttta gaaaaagagc atttgtttca
    22561 gataagcgaa atgttgagaa acgaacagtt tgatccaaat caatttttag ttgttgagta
    22621 cccagctact ggtgtgtatt gttgtaatca tgtgaagggt gaaaaatatt ttattattca
    22681 agaatacgaa ggtaagttag cgccttatta tactacttgg gaaataaatg aagagggaat
    22741 caataatttc ccatgtgaaa gcattgaaga gtctatttct ctcacggaat gttaaataaa
    22801 tagagacgta aaagagtcct gaacgtgatt gtccaggact ctttttcaat gaattgtttt
    22861 ccgccaattc actgagtggt gcgattcaat tttattatat caaaagaaag tgagattcca
    22921 aagtgaaggg aggaagaaga aatgactcag acaacgattg gtgttgtggt tgaaaaaagc
    22981 agggataatc tcgtttttgt gacagaaatt caaacaggta gagcttttgt agtcacagac
    23041 aaagctgcaa aagcgtatca aaatggcaat attttaacgt taaatctgac tacgaagatg
    23101 ttcgttgatg cagtagaaaa ttatccattt gtttagacaa acgatatttt acaaggagga
    23161 aacggacatg gcagaagaaa aagaaccact agataatagt cgtttgggaa agtccaaaag
    23221 aaaattagta cggttgcaaa acgaattaaa taatcaattt caaaagctat ttgatcacca
    23281 aaaattgaca aatgggcaac caatgaatga caaacgaaat ggtcgttcat ggtttaacca
    23341 gcaagaaaaa atagaaaata aaatacaaag tttgagagaa gagatcaagc aacaagaaaa
    23401 acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa atgggttata acaagtacgg
    23461 tggcctggac atgacgatcg aaaatattcc aagaatcaaa gaagaaatcg aacgctttga
    23521 aaaaggtgaa tcgacttttt cagctgccac gatccgaaaa taccaacgaa aattggaaac
    23581 attagaacag ctgaaagaaa aatcagagaa aggaaaagaa aatcttcttc ctgaagtaca
    23641 agcgatcatt gacagcggac gagtgacgca atggaaaaag aatccaacta tctattttct
    23701 gaaaggctat cgaaaagtcg ctttagaact caatgaaaac gggggatttg aggaatctaa
    23761 aaaataccaa gccacgtcgg aagaagaaaa ggcagctgtt cgtgaattac ttgaagaagc
    23821 tacacctact gattctgtga aggagaaaga aaccgatcat ttctatcgag tagaattcaa
    23881 cgaaaatgat ccagcaaaag ggttgaaaag ttatggtggg gagatcgtaa cactagaatt
    23941 gatcgatcaa ctcaaaacac tggataacca atacgccgca gaagcaggct attataaatt
    24001 ctattttgaa gaagtgaaga atggagaagt agtcgatcag atacgaatgg atctagggga
    24061 tggcgtcaat agtaatcacc ccatctattt agagttagct agattgtgcg aacaatcacc
    24121 tgaaaaggaa caagaacaaa cagtcaaaaa tgaacctatt tcaaaagtac aaaacgagat
    24181 taaagagaca aaaacaaaac aaaagggtta tcaaaaacga acggcagaag agattaagca
    24241 agaagtcaaa tcactttccc aacaggcgct tgatgcggtg aaaaagcata ctcattcacc
    24301 tgaagacgtc aaagaacttt tgaattttat gtctcgtttt ccagaacgat cctttagaaa
    24361 tcaattattg atcgaggaac aatttccagg cgcaacggct tgtttaggtc gtgccgcgtt
    24421 gaaaaaagaa ggaatctata tcaaaaaagg agaacaaggg aacaagatct ttgtcagaaa
    24481 aacggtcaaa ggattttatg agaaaggacg tggctttgtt cgagaaacag aggctacacc
    24541 agaagataaa aaacgaatcg aaagtggtca aatcgaagtg atcaaaaaac cttactacac
    24601 gattgaaaaa gtgtttgata tcacacaaac acaattgaaa cctgaagact atccgaaaat
    24661 cttcccgaac cgcgtctttg attttcaatt ggatcaaaca gggcagacag aacttcaagt
    24721 tgggattcaa gcggtagcca aaaatattgg aattcaaatt cgagatatga cagaaagtga
    24781 agtctatcaa agagaattag gacaagcacg tggggcgtac gttcgtaacg aatttactgg
    24841 cgaagaagaa atcgtcatga atactcgaaa tactcctacg cagcatttag ccacaagtat
    24901 tcacgaatta gcccatgcta gaatgcacaa attttcagaa ttagatactg caacaaaaga
    24961 actacaggca gaaatgacct cctatattgt gtgcaagcat tttgggatgg acacctcgga
    25021 gaaagctatt ccttatattg cgacatggac gaagaatagt cagacgttgg atgataagga
    25081 agctgcggaa cgtgggaaaa tcatgaatga tgtcagtcgt gtcgcaaatg aattcattca
    25141 aaccatttct aatgaaatca atcaatacag agaaagagaa ccagagatcc agccagcaga
    25201 agtgaagcag gagcataaaa atactgtaga aacggcagaa caaaagaata ttgtgcccgt
    25261 tggctcactt tggatcgaca agaaagacgg gaaagtcctg gaagaagaca cgggccgaac
    25321 gttacatttg aaggatgcgg cgtttattga agatccgcag cataatcaag tgattttaaa
    25381 aggaaaaagt tatggaaaag agatccaaac ggcgttacct tcagaagaat ttgcgctagg
    25441 aaatccattc cgattttcac ttgagacagt gtcagaagtg gcaaaacgaa aaaatgaaat
    25501 ctattatgaa caacaattgt cacaaaaaga acgcgtataa aacaagcaag aaagactatc
    25561 tgaaaaatgg atagtctttt ttcttttggg aggtttgaaa atgacagaga aaaaaatgag
    25621 tctcattgat cgatgcaagc aaatggatat tgtggatttt gcacgaaaca atggtttagc
    25681 ggtgg
//
LOCUS       DAPCMT010000026        25060 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPCMT010000026 DAPCMT010000000
VERSION     DAPCMT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.026"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            12..84
                     /locus_tag="RD743_000890"
     tRNA            12..84
                     /locus_tag="RD743_000890"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="RD743_000891"
     tRNA            88..160
                     /locus_tag="RD743_000891"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="RD743_000892"
     tRNA            168..249
                     /locus_tag="RD743_000892"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="RD743_000893"
     tRNA            267..339
                     /locus_tag="RD743_000893"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="RD743_000894"
     tRNA            351..422
                     /locus_tag="RD743_000894"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="RD743_000895"
     tRNA            439..524
                     /locus_tag="RD743_000895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="RD743_000896"
     tRNA            535..608
                     /locus_tag="RD743_000896"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="RD743_000897"
     tRNA            618..691
                     /locus_tag="RD743_000897"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="RD743_000898"
     tRNA            711..783
                     /locus_tag="RD743_000898"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="RD743_000899"
     tRNA            811..884
                     /locus_tag="RD743_000899"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="RD743_000900"
     tRNA            898..971
                     /locus_tag="RD743_000900"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="RD743_000901"
     tRNA            995..1084
                     /locus_tag="RD743_000901"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="RD743_000902"
     tRNA            1097..1170
                     /locus_tag="RD743_000902"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="RD743_000903"
     tRNA            1189..1264
                     /locus_tag="RD743_000903"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="RD743_000904"
     tRNA            1266..1336
                     /locus_tag="RD743_000904"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="RD743_000905"
     tRNA            1350..1423
                     /locus_tag="RD743_000905"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="RD743_000906"
     tRNA            1448..1535
                     /locus_tag="RD743_000906"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="RD743_000907"
     tRNA            1557..1628
                     /locus_tag="RD743_000907"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="RD743_000908"
     tRNA            1651..1740
                     /locus_tag="RD743_000908"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="RD743_000909"
     tRNA            1753..1826
                     /locus_tag="RD743_000909"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="RD743_000910"
     tRNA            1845..1917
                     /locus_tag="RD743_000910"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="RD743_000911"
     tRNA            1921..1993
                     /locus_tag="RD743_000911"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="RD743_000912"
     tRNA            1998..2068
                     /locus_tag="RD743_000912"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="RD743_000913"
     tRNA            2081..2154
                     /locus_tag="RD743_000913"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="RD743_000914"
     tRNA            2166..2237
                     /locus_tag="RD743_000914"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="RD743_000915"
     CDS             2503..3696
                     /locus_tag="RD743_000915"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDU0562744.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="RD743_000916"
     CDS             3826..5298
                     /locus_tag="RD743_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0562745.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="RD743_000917"
     CDS             5381..6169
                     /locus_tag="RD743_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDU0562746.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="RD743_000918"
     CDS             complement(6236..6904)
                     /locus_tag="RD743_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDU0562747.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="RD743_000919"
     CDS             7019..8731
                     /locus_tag="RD743_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562748.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="RD743_000920"
     CDS             8734..10503
                     /locus_tag="RD743_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562749.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="RD743_000921"
     CDS             complement(10708..11274)
                     /locus_tag="RD743_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDU0562750.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="RD743_000922"
     CDS             complement(11274..12230)
                     /locus_tag="RD743_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDU0562751.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="RD743_000923"
     CDS             complement(12230..12886)
                     /locus_tag="RD743_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDU0562752.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="RD743_000924"
     CDS             13256..15274
                     /locus_tag="RD743_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDU0562753.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /locus_tag="RD743_000925"
     CDS             15701..18115
                     /locus_tag="RD743_000925"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDU0562754.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="RD743_000926"
     CDS             18463..20016
                     /locus_tag="RD743_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDU0562755.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="RD743_000927"
     CDS             complement(20397..22181)
                     /locus_tag="RD743_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562756.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="RD743_000928"
     CDS             complement(22196..23917)
                     /locus_tag="RD743_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562757.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="RD743_000929"
     CDS             24017..24778
                     /locus_tag="RD743_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDU0562758.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7735 a         4618 c         5342 g         7365 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta
//
LOCUS       DAPCMT010000027        24609 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPCMT010000027 DAPCMT010000000
VERSION     DAPCMT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.027"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            117..494
                     /locus_tag="RD743_000930"
     CDS             117..494
                     /locus_tag="RD743_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562759.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(674..1969)
                     /gene="purB"
                     /locus_tag="RD743_000931"
     CDS             complement(674..1969)
                     /gene="purB"
                     /locus_tag="RD743_000931"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDU0562760.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1982..3106)
                     /gene="purK"
                     /locus_tag="RD743_000932"
     CDS             complement(1982..3106)
                     /gene="purK"
                     /locus_tag="RD743_000932"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDU0562761.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(3099..3587)
                     /gene="purE"
                     /locus_tag="RD743_000933"
     CDS             complement(3099..3587)
                     /gene="purE"
                     /locus_tag="RD743_000933"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDU0562762.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3880..5187)
                     /locus_tag="RD743_000934"
     CDS             complement(3880..5187)
                     /locus_tag="RD743_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDU0562763.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5190..5771)
                     /locus_tag="RD743_000935"
     CDS             complement(5190..5771)
                     /locus_tag="RD743_000935"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDU0562764.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5887..5984)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6343..7635)
                     /locus_tag="RD743_000936"
     CDS             complement(6343..7635)
                     /locus_tag="RD743_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0562765.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7632..8255)
                     /locus_tag="RD743_000937"
     CDS             complement(7632..8255)
                     /locus_tag="RD743_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562766.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8258..9094)
                     /locus_tag="RD743_000938"
     CDS             complement(8258..9094)
                     /locus_tag="RD743_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0562767.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9111..9998)
                     /locus_tag="RD743_000939"
     CDS             complement(9111..9998)
                     /locus_tag="RD743_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0562768.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(10006..11241)
                     /locus_tag="RD743_000940"
                     /pseudo
     CDS             complement(10006..11241)
                     /locus_tag="RD743_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(11924..12607)
                     /locus_tag="RD743_000941"
     CDS             complement(11924..12607)
                     /locus_tag="RD743_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDU0562769.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12625..13242)
                     /locus_tag="RD743_000942"
     CDS             complement(12625..13242)
                     /locus_tag="RD743_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDU0562770.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(13252..14076)
                     /locus_tag="RD743_000943"
     CDS             complement(13252..14076)
                     /locus_tag="RD743_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDU0562771.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(14069..14629)
                     /locus_tag="RD743_000944"
     CDS             complement(14069..14629)
                     /locus_tag="RD743_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562772.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14803..15759
                     /locus_tag="RD743_000945"
     CDS             14803..15759
                     /locus_tag="RD743_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0562773.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(16064..17020)
                     /locus_tag="RD743_000946"
     CDS             complement(16064..17020)
                     /locus_tag="RD743_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDU0562774.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            17124..17726
                     /locus_tag="RD743_000947"
     CDS             17124..17726
                     /locus_tag="RD743_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDU0562775.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17814..19313)
                     /locus_tag="RD743_000948"
     CDS             complement(17814..19313)
                     /locus_tag="RD743_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDU0562776.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19329..19853)
                     /locus_tag="RD743_000949"
     CDS             complement(19329..19853)
                     /locus_tag="RD743_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDU0562777.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            20151..20900
                     /locus_tag="RD743_000950"
     CDS             20151..20900
                     /locus_tag="RD743_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDU0562778.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20891..21676
                     /locus_tag="RD743_000951"
     CDS             20891..21676
                     /locus_tag="RD743_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDU0562779.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21645..22361
                     /locus_tag="RD743_000952"
     CDS             21645..22361
                     /locus_tag="RD743_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDU0562780.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(22463..23497)
                     /locus_tag="RD743_000953"
     CDS             complement(22463..23497)
                     /locus_tag="RD743_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDU0562781.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23501..24559)
                     /locus_tag="RD743_000954"
     CDS             complement(23501..24559)
                     /locus_tag="RD743_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDU0562782.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7322 a         5275 c         4294 g         7718 t
ORIGIN      
        1 tgttttatca gaaacgaaaa tcttatacca tcaacaagcg tttacattta tacttaatga
       61 tggtatatta gaattaacta aaaatttgga taatgggttc gaggaggcac ttgccgatgg
      121 aagatgcaat aaaacaaggt cagctttttt tatcagaatt aacagctgaa gaacgttcct
      181 gtctatataa tgatttgctg tcgatcaaag acgtttattt atgcaataaa gatactagtt
      241 tttatacatt ccgaaaaatc cgccgagcaa ccaagcgctg tgctgaatgc cgtaaaagga
      301 cttttcttta tcagacagaa cacaaaggcg aactttcaat cagtcagatt catgaacaga
      361 agcgtttcaa aaatcattat cgtatccaga tcttattcaa tggacaagag atggaatacg
      421 cttttctcga aaacttgctg caacatctcc ataaccagca ttttagttat aatagccaag
      481 ccatttacgc ttaattcctc tattttttgt atgtaaagag gcacggtaaa gaggtagccg
      541 cttttaaaat caggttccta ttattgataa gaaaatataa taaactaaaa gaagcagaac
      601 cgctaacgga tctgcttctt ttttggttat ttttgctatt ctattttaat tgaattttgc
      661 aactcactct tctttattcc aaacctactc gtttgaatat cacgtcaacg tttttcaaat
      721 gatgatgata atcaaaggca tcgtctattt cttctttaga taataccgat gtgatggttg
      781 gatcttcttc taacagcggg cggaactgca cttggttgtc ccaagcgtac gcagtttttg
      841 gctgaacaag atcataagct tcttctcttg tcattccctt atcgatcaat ttcagcataa
      901 cgcgctggct ataaatcaaa ccaaatgttg cagacatatt ccgcttcatg ttttcaggaa
      961 agaccgttag atttttaacg atattgccaa aacgatttaa catgtaattc aataaaatcg
     1021 tggtatcagg caagatgatt cgttctgctg aagaatgaga aatatctctt tcatgccata
     1081 atgtgacatt ttcataggca gtgaccatgt gtccgcgcac cactctggct agaccagcca
     1141 tattttctga accgatcgga ttccttttgt gcggcatagc agaagaacct ttttgacctt
     1201 tcgcaaaaaa ttcttctact tcacgtgttt cggatttttg caatccgcgt atctctgttg
     1261 caaaacgttc aatacttgta gcaataagcg ccatcgctga gaaatattca gcatgtagat
     1321 cccgagggag aacttgagta gagatttctt gtggacggat ccccaattgt ttacatacat
     1381 actcttcaat aaatggcggg atatttgcga acgttcccac tgctcctgag atttttcctg
     1441 cttctactcc ttttgccgca tgttcaaatc gttcgatgtt ccgtttcatt tctgaatacc
     1501 atgtggcaag tttcaatcca aaagtagttg gttcagcatg gacaccgtgt gtccgaccca
     1561 tcatcactgt atatttgtgt tcttttgctt tttcgccaat gatttccgta aaacgtttca
     1621 agtcttcccg taaaatatca tttgcttgct tgatgagata gccataagca gtatctacga
     1681 catccgtact tgttagacca taatggaccc attttctttc ttctcccaat gtttcagaaa
     1741 ccgctcgtgt aaacgcaacg acatcatggc gtgttaattt ttcgatttcc aagatccgat
     1801 cgatatcaaa tgatgcatgc tcacgaattt ttttcacatc ttcttgcggg atttcaccaa
     1861 gttctgccca agcttcatct gctaaaatct cgacttcgag ccacgcacgg tatttgttct
     1921 cttccgtcca gatattcccc atttcagggc gtgtataacg ttctaacatg ttttttctcc
     1981 tctaatccca aatattcgtt tgtttgattt cttccagcgt ttgctggatg tcttctgtca
     2041 agatagtgat atgtcccatc ttgcgacctt tttttgcttg tttttttcca tagaaatgga
     2101 attgccagtc tggcttattc tcaatcaagc gatagctttc cagcagttgt tctcccaaaa
     2161 cattgaccat tactgcattg cttatcaaac gtactttttc tcctaatggc caaccacaaa
     2221 tacctcgaat atgggcatcg aactgactca tagaacaagc ttcaatagaa taatgtccag
     2281 aattgtgagg acgaggagct aattcattca catatagccc gcctgttttt gttaaaaaca
     2341 tttctacccc taaaacacct gaaaggctca ctgcttctgc aatcacacgg gcaatacgct
     2401 cagcctcctc gatcacatcc tgatcaatcg cggctggagc aatcgtttca tgaagaatat
     2461 tgttgtggtg gatgttttcc acgaccggaa aagtcgtgaa atctccttga ccatttcctg
     2521 ccaccatgat cgaaatctct ttttcaaacg ggatccatgc ttctaaaaca caggttcctt
     2581 ctcttaaaag gtccatggca ggagctagat ccgcccgact tttcaaaaca tactgcccct
     2641 tgccatcata gccgcctcga gtggttttca aaacacaagg atagccgatc ccgtcaatcg
     2701 catcttggat atccgtcgga ctgacgatcg ttgcataagg agcaatcacg atgttattcg
     2761 tttccaaaaa agatttttct aacaggcggt cttgcgtaat cgcaagcaag tctgtccctt
     2821 gaggaataaa tgacatgggt aaaatcgcat ttaacgcctc tacactgacg ttttcgaatt
     2881 cataggtaat gacatctgtt cttctggcaa gctctttcag cgcaaaggtg tcatcataga
     2941 cactttcaat gtgccaatct gcaacttgtg cggctggaca atcgctcaca ggatccagca
     3001 cacctacttt aaagcccatt tcttttgcac taatcgccat catacgacct aattgaccgc
     3061 caccgacgat cccgatcgtt gaaccaggca acaaaggttt aaccaagttg atcactactt
     3121 tctataacag ttttagccaa cattgaacgt ctttcagcca atttagcctc caattctaaa
     3181 tcatacatgg aaagcatctg tatcgcaagt agtccagcat taattgctcc cgcttttccg
     3241 attgctgtag tagctacagg tactcctcca ggcatttgga caatcgaaag aagcgaatcc
     3301 aaaccattta atgttcgtga ttggaccggt acaccgataa ccggcaaagt tgtttttgat
     3361 gcgaccatac ccggcagatg cgcggctccc cctgctcctg caataatgat tttgattcct
     3421 ttttttcgag cgttttgtgc atattcaaac atcaaatctg gggttcgatg agcagaaacg
     3481 accttttttt cataagacac accgaattca tccaatattt gacatgcttc tttcattgtt
     3541 tcccaatctg aagtactccc cataatcaca gagatttttg ctgtcattgt ttttcctccc
     3601 agtttttcta cattcatatt ttaacaatca ctttttttga tgtcaaagaa atttcgaatg
     3661 ttaacaaaga aaaacatttt attgttcgtg tttattaaag atgaaaaaaa ggaatgtgac
     3721 aaaagttagg tcacattcct ttttatggat aaacggtgtt caatcagctg accctcggta
     3781 ttagctcaaa caaacggcaa aatacgagga actatttttc cgtttgtttc tctaacaatc
     3841 aggtcaaaac gctgatttca caacctttct tttgctctat tacttcttga aggcttcttc
     3901 caatatttct tcatcttttt tcaatccatt aaagatcaaa ttcaatatga tagccgtaat
     3961 gctactcatt acgatgccat ttccagtgaa catttgcaat gtttgcggca tttgactgaa
     4021 taatgtcggc gtaacattga aacccagccc aaatccaaca gaaacagcaa caatcaatag
     4081 atttttatca ttgctgaaat caacttccaa cagcatacgg attccttgga ctgccaccat
     4141 cccgaacatg actaacattc ctccaccaag taccggctca gggatgattt gtgctaacgc
     4201 accgatttta gggaataatc ctaaaacaat caagaagaat gcagagaaat agatgggttt
     4261 tcttgttttg attcccgaaa gttgaaccaa tccgacattt tgagaaaagc cagtataagg
     4321 gaatgtattg aaaataccgc ctaagataac ggcaagtcct tctgcacgat atcccttttt
     4381 caattcttct tctccaacat ttttcccagt aatgtcacca agtgcaaaat atacacctgt
     4441 tgattctacc atgctgacca tggaaatgat gatcattagt aaaatcgacc atacgtcgaa
     4501 agttggttta ccaaaataaa agggttgtgg aacatggaac aacggcgcat ctccaatcgc
     4561 gctcagatca atcattccca tgaaagcagc taagatgctt cctccgacta aaccaatcaa
     4621 taccgcgatt gaacgaatga acccttttgc cagcatttgc acgcctacaa ttaggagaat
     4681 cgtcacaaat gcaagtagca gatttgtttt gtcaccaaaa tcagctgctg ttgcattacc
     4741 tccacccatt ttctcgattg ctacaggaat caaagtcaaa ccaataacgg tgataaccgt
     4801 tcctgttact ataggaggga atagtttttt aattttagaa aagacaccag aaactaacac
     4861 aacaaagatc ccagaagcga taatagatcc gtagatagct cctactcctt cattggttcc
     4921 gatcaagatc agtggagcta ctgcttggat cgcacatcct aaaacgacag gcaagccaat
     4981 tccgaaaaat cggttaactg tcaattgcaa taatgtcgct aatccacaca taaagatatc
     5041 aattgaaatc aaataagtca tctgttcttg attaaagcct agtcctgtac cgatcaaaag
     5101 aggcaccgct actgctcctg catacatcgc tagcagatgc tgcagcccta aaaccgcagc
     5161 ttttccattt tgtgtttctt tttccacgat tatgcgtcct cctcaagaaa ctcaacttgt
     5221 ccattcgtca aggaagcaat acgcgcaaga gaaacgactc tcaatcccat ttcttcaagt
     5281 aactgacgtc catcttggaa agacttttcg atgacgatcc cgattccttc tacttgcgct
     5341 ccagcttgct ggcataattc gatcaatcct tttgccgctt gtccgtttgc caagaaatcg
     5401 tcgataatca atactttgtc ttctgaagat aaaaatttac gagaaataga aatcgtactt
     5461 gtcacttgtt tcgtaaaaga ataaacagaa gctgtcaata attcttcatc catcgtcaaa
     5521 ctttttgctt ttctagcgaa gatcattgga acatctaatt ctcttgcagc aaataaagct
     5581 ggtgcgatcc cagaagcttc aatggtcaca actttcgtga ttccttgttc tttaaatact
     5641 tctgcaaacc gttttcccat ttgttccatc aatacagggt ctacttggtg agtgacgaag
     5701 ctgtcgactt ttaatacgcc ctcgcctaat atacgtccgt ctttttgaat acgttcaact
     5761 aattctttca ctttatttgc tcctttatca tttattaata ccgaaataac cgcttttgtg
     5821 caaaaaccta ctatgtttct gttaaagaaa aacaccctcc actttatcat aagaaaagaa
     5881 gggtgaaata gccaatcttt tacttatagt ccagcattta cggtactggc tagaaactgt
     5941 catccatatt atgacgatat ataagtaggt atgtaaaact aataatagtg taaattcatt
     6001 ttcttgcaac tgttttgcac gtgaaggcag tttgccatat ttgaataaaa aatgttgttt
     6061 caaatattga aaacagtcta acactacttt ctgaaactga caacgtggtt tacttatttt
     6121 tttgtaaaat ctaatgattt ttaatgcatt attccttttt gttcgggttt aaatagttat
     6181 ttttattttc tcctatctaa aaaagcagcc cacataaaaa gaaggcatga caaaaagcat
     6241 gtcatagccc ctttttccga ataaatagtg ttcaaaagac agcctatcgg caataaggca
     6301 agattttata aactacggga agtaatttcc gtaaatctat tcttattctt ttaagctgac
     6361 aagctcgtca atcactactg cttttcctga agcaatagaa tgatttgctg cgattcctgt
     6421 caaaatcgac aaaataccat ctacgtgagt tgctgcccga ttccacggat cttcttcttt
     6481 ttcttcacca aatagatcat ctaataaacg cggatctcct ccgccatgtc cgcccgcttc
     6541 ttgttcgacg attacctcat atggctcacc gaacatcgga tagacatgga tggactgaag
     6601 aacggctgct ccttcatcct ttttatctcc acctgcattg atgtaagact tttcgatcac
     6661 tttcatttcg atccgtcctt tgctgccatt gaaagaaaca ttgaatcctt cccacggcat
     6721 atacgcgttt agcgaatacg tcaaaatcgc accagtttca tatttgaccg ttacgcctag
     6781 tgtatcttca atagagatat catcggaaaa tacactgcgg tcacgaatat atccactgtc
     6841 tttttctgct tctaaataca tttctcgcaa ttcctcattg gctgacaggt ctatagcaaa
     6901 tggatcagct ttagctagtt cgcttccata gcatcgactg taaaatattt ctactccgcg
     6961 tttctccgca ttatgcatcc cataaaagtt taatccaccg aatgcaaata ccttttgggg
     7021 acggctgttc aaccaaaaat tcactagatc aaaatgatgg gtagatttat gaacaagaag
     7081 cccgccgcta ttgtgcttat ttcgatgcca tcttctgaaa taatctgccc cgtgttgcgt
     7141 atttaacagc cattcgaagt ggatagaaaa cacatcacca atcacatcac tttctatcag
     7201 ttcctttatc ttcgtgtgat acggtgcata acgataatta aaagtgactc gcaggcttct
     7261 tcccgtctgt ttttggaccg ttattatttc ttgagctttt tcctcatcga tagtcagagg
     7321 tttttcgcaa ataacatcat atcctttttt cattgcacga atgatataat catgatgcgt
     7381 tcgatctact gacgtaataa tgacttcagc cggccgtacg tcatccagta gttgctcaaa
     7441 ttgtgatggc atacatgtct ttactttttt ctgtccatac ttgttcacaa gtatccgatt
     7501 tgcataatcc atccttgttt gagatacatc acaaaaaccg actagttcac aagtttgatg
     7561 atacgtagag gcaattgcct cataaaaaaa acgcgctctc ccaccaagtc cgacttgtac
     7621 atattttttc ttcatttaaa gtcattctcc ttttctttta agcaaaatac tgtgacaaat
     7681 ggcagccctg gatacagtaa tgcagtgagt aaaaaatcag gctgtataca ccagaacagt
     7741 ccatacaaaa ggcataaata ccacagaaac cccttgacgt tttttgacaa cagccagata
     7801 ttccttttga tcaaataaac aagactatct gctttatctt ctttgcagat atcaattagc
     7861 gtactaaaaa aactatggga tacaaaaagc aacagacaca atagaaaagg aatcagatac
     7921 caagctccaa tctttcccac taataaatac caatcaactg caaaaataat gacaaacagc
     7981 tgaaacaacg taatgatgaa acacatccgt ttgattttcc ctttcctcca acataccttt
     8041 ttttctgcca agtaatggac agatagataa taagaaggat acatcatcaa aaaataaatc
     8101 agatagacaa cacgagcaga cagcactaat cctaaagtga acaaacatcc tagaactaaa
     8161 gcgaaaagag cattactgat aagactggtt aaaaaaatcc gacagacata gtagttgagc
     8221 ttgtaaagaa acatctcttc tattggcttt ttcatattca gcccttcaga ccgcttgtac
     8281 tgatcccctg gacaagatat ttattgaaga ataagaaaat cagaaaaacc ggtagcagcg
     8341 acaacgtaga catcgcaaac atactcgcgt aagtcgtact agatccagcg tctgcaaata
     8401 atttgatggc aatcgatacc gtgtaagatc tcggcttgtt taaataaatc aatggtccca
     8461 tgaagtcgtc ccatttccaa taaaactgga tgatgatcgt cgtgatgatc gaaggtttca
     8521 gcaaaggaaa aataattttg gtaaagacac tatacttgct gcatccgtca ataatcgctg
     8581 attcatctaa ctctttgggg atgctgacca tgaattgcat gatctggtaa atgaaaaatg
     8641 cttgtccaaa aaaatgtggt aaaatcagcg gcacatacgt cccaaccaaa ttcagccgat
     8701 tgtaaataat aaactgcgga atcaaaatca cttgtgcagg aatcatcatc gttactaaca
     8761 tgacgacgaa ccagaatctt tttcctttga aatcgattct cgataatgcg taagcaatac
     8821 aagtcgagct cagaactgtc cctatcgtcg aaactgcaga gatgacaaac gaattcctaa
     8881 agaaagtaaa aaatgaaatt ccagcaaacc ccgcccatcc ttttgcgtaa ttttcccatt
     8941 gaaaagtctc tggaatcaaa ttcagcgaac cactcataat ctcttgatcc gttttaaaag
     9001 aacctgcgat catccataag accggataaa tcatgactag acctaatagc aaaataaaac
     9061 cgtattgaaa ccatttcgtt actgtattat tcatttcttc ctcctctcat tcacacaacg
     9121 acccattttt ttgatgattt gaaaatcaga aaggtgacag ctcccataat caacagcatg
     9181 atccacgaaa tagcactagc ataccccata gaattaaaag aaaaagcttg gttatacaca
     9241 taaagagcat aaaagttcgt ggcatcattc ggaccacctt gggtaatgat aaatgcctgc
     9301 gtaaaagtca taaacgccgc gattgtctgc ataaccaagt tatacaaaat gatcggcgaa
     9361 agaagaggta acgtgatctt gtaaaacgtc tgccatttgt tcgctccatc gatctctgcc
     9421 gcttcataat acgagcctgg gatctctttg atacctgcag caaagatgat cattgccgaa
     9481 ccgaattgcc aagcagacat caaaacaaga gggatcatga ccagtttttg atcgctaaac
     9541 caggaaagcg caggtgcgcc taatcgttcc aggaaaatat tcactaaccc gtttttagaa
     9601 aacaactccc gccaaactag agcgatagaa atactgccac cgatcaaact aggcacataa
     9661 tacaaagaac gaaagaaagt cgttcctttg atattcctcg ttaaaagata agcaatcaat
     9721 agaccaaaga tcagtttcac aggtacagat gcaaatacat agagaaaagt gactcggatg
     9781 gagttcaaaa aacgggcatc actcagcatc tttatataat tatccaagcc gatccatgag
     9841 actggatttc ccaaacgata atcggtgaac gaataataaa gtgacaagat catcggtatc
     9901 actgttatca atagaaatcc taggatgaat ggagaagcaa atacgtaacc gaccacattg
     9961 tcttgatcca agaaccgttt tagtttttct tttttcatgc cttccttctg ctgttacttc
    10021 ttcataaatg actttttcaa ctaaaagatt gtattggtct tgaatttccg gattcaaagc
    10081 actatcaaag acattcactt cgccgttgtc catatcggaa atcatttcta aataatcaaa
    10141 tgtcgcttgc aaagttgcat catctgcacg agcgagctgt tctcgaatat tcgacatgat
    10201 tggtacacca cgttctcctt ttaaaatttc attggcttct tcactgtttt gaaagaaatc
    10261 aataaactta gcagcttctt ctttatgggc tgagcttttt gccatactca gcatttgcga
    10321 agaacgtaaa gggataccag attctccatc tgcttttctg cgaggtggat taatcaactg
    10381 aatctccata tcttcaggag cccctgacaa tatttctgtc aactggttac ttgatagaaa
    10441 caccattgcc gcttctctat ccctaaccgc ctgaaaagac agatccgtcg tacttgcagc
    10501 tccaccacct ggttctggat gggccccttt gtctaccaga tttttacgca gctggaaaaa
    10561 atctgttaga tagtctggat tttcaaatcc caagctgtta gatagatctt cccgattata
    10621 aaaattcaat cctttctcaa cttgtggtat ttgaataata cagccagcta taaaatcttc
    10681 gaatcttgaa atagcataga ttcctaattc ttcagtgatt tgatagctga tttgttcaaa
    10741 ttcatcccat gtccaatttg aagctggttc agggatattt aattgttcaa atatattttt
    10801 attgtaagca atcgcaaacg aattcacccc gttagacagt ccaactaatt gcccgtcgta
    10861 agtcgtcgtt gctaaatagg attcatctgc attagaaata tcaattatcc cttgctccac
    10921 atattcatct aatggttcaa tctgattgat gtattgagga aattgattgt tcagttggaa
    10981 aatgtcccag atatcatctg atgctgctag tgtacctaat tttgtgtaat atccttcata
    11041 atcataaaat tcataatcga tcgtcacatg gggatttttt tcttcataaa gctcgattac
    11101 ttttactgtt gcatcgtgtc ggacttgcga accccaccaa gccatgttca gcgtgatttt
    11161 tttgtcatct tcttgcccac aagccgtcaa gaagaataac ataacactta aagctaagat
    11221 gattctcatt cttttcttca ttctctgcac ctctacttcc attgttattt ttcaatatac
    11281 tgtattttta tacagaacat atttattaaa cgatcttttt caaggggaat aaaaaccaag
    11341 acttgcctta tcgttctgcc taggatgaac aactcaatac tttcaagaaa aattagatag
    11401 tgctgattct tctttcatcg ctaaaaacac ttcttgcttc aatgatggga ggataattac
    11461 gcaaagaaaa aaggatatca gacaactgcc caatatcctt gacatagcta aataagcatg
    11521 cttcttcgtg tgcgctcttc cctaaaaaac agaaaaataa ctagtaattc tgtatgaaat
    11581 taggtacaat atacacgtcg atgcacaagc attgactatg gcaacttgat tggtcagttc
    11641 ccgctgactt ttcaagcccc gacaatttag ctgctactaa attgtcgggt gcctttttta
    11701 gtttttattc gtttttcatt ctatagaaag cgcttcaata aatcaagaca tttttttgtt
    11761 agaatgctaa taataacaaa aggttttaac tcttcttatg tacattacag gtaaaaaaac
    11821 aaaaaaattt ctcttttttt tgaattctga acaaatagta ctggagtaca aaagacgagg
    11881 aagagccact atctgctctt cctcgtcttt tgattctcac ctgttattca tgattctttt
    11941 tctcagtttc ccgatatttt tccattttac gactaaaaag ctctccgcta cttggtggcg
    12001 tatatgtgat ggcatttgct cctgcttgaa tggtctcagc aatactttct tccgttggac
    12061 cacctgtcgc aatgatagga agttcaggat attttttacg aaaatggcgt actgcttctg
    12121 cagtttcttt acctgcactg atattgataa tcgaaatacc agctgctaac ttttcatcta
    12181 tttctgtata tttcgacgtt accgtactga taactggaat atcaactact cgacaaactt
    12241 cttttactgt ctctaaaggc gtaggaccgt tcaaaacgac ccctaacgaa ccttgcgctt
    12301 ctgcgaacat gctcatgtag gaagaacgag caccttgtgt caaaccacca cctacaccgg
    12361 agaaaactgg aatgtcagct gcctcaataa tgcttttagt aatcgctgga tgaggtgtaa
    12421 aaggatacac agcaattact gcattcgcat ttgtattacg tatgatcgca atatccgtag
    12481 taaagatgat cgaacggatc aattttccaa atatcttgat cccgcttgct tctaaaatga
    12541 tttccggtac tcttacgata tcttttctca attccgtcat gatttctggc gtaaattgtt
    12601 ctgccatcag ccacttctcc tttgctatct ccggtcatac tgattcaatt gatacgtctc
    12661 gatgacctgg atgatttttt gcgcataacc ggggtctgtc gcataacctg catcttgcaa
    12721 tgcttgtgcg gcttcctgat agttggtggc cgtaatcact ttttcataca gcgcaggatt
    12781 ccaatctact ccttggacga acagttgtgt atggtcatcc atggactgtt cccatgaatc
    12841 atacactttg aagtctcctt ggatagttat ccattcttcg ttaacaaatt cctttgtttc
    12901 caggcttact ttcttctgat ccccataagc cttgatcccg aaaagattat tgtatttgct
    12961 agccaaggta ctttttcccc aatttgattc taatatagct tgtcctaaaa tgatactagg
    13021 aagcactcca taaccatctt gtagttcttg ggcatgcgga acaagccgtt caataaattg
    13081 ctgatgggat tgcgcttcat tttcttgctg attatcagta aaaggagaag acagattctt
    13141 caaggaaaaa acaaaggcta agagtatcac tgctacaccg gcaaccactg ctggccaacg
    13201 gatattcttg cgcttttgct ttttccactt ttttttcgcc atgatcaatt cctacttttc
    13261 atttaattca ttcagatatt cttctagcgt caattggtgt ttggtgatat actcagcgtt
    13321 ttctttaccg acataacgta agtgccaagg ttcatatgtg atcttagtta tatcttcgtg
    13381 tccttttgga taacgaacga taaatccata ctgcgcagca ttatctgcga tccatttagc
    13441 tccaggctga tctccgtaac ttgcatccaa tacagtagaa ggataactgt tgtaccagtt
    13501 ttgatcgacg acatcgactg ccagacctgt gtggtgttcg ctgtatcctg gctccgtaat
    13561 cgtttcttta gtaatctttg tcgcctcctc ttctgataca ccttgacgac tcatcacatc
    13621 ttggacattt tgttcgaaaa cttgttgttg ggatgataca gaccggtaag ctgagaccat
    13681 cactaatgga aatccagctg cttcagcagc cttggcaaat gcttcatatt ctggagcaat
    13741 gcgtttgtcg atcaagtatc cgttacttaa tgatgccaat tgagtagact catcaatttc
    13801 tttttctaat ttgtgatctg gaccaaccaa aactagcttc caatcgtctg ttttaacatc
    13861 tggcaattgt ttggacgtac gatcttttgt ttctttgtcc tttaccttgc tgctggttgt
    13921 ttgactgttc ttttctttac tagcagttgt tttggtgctt gttcgttcga ctgcttgatt
    13981 gccggcttga acatgcgaat catcatcgct aaaaataaca tagcccattg ctgcgatcat
    14041 aatcacaact gctccaaatc ctaaaaattt attcacttga aaaatccttt cgttgttctt
    14101 cacgttcccc attcgcctca tgaacgtaag tttcttccag attttcattc acccaatcga
    14161 gaagttcgat tttttctcct tcgatctgtt tttgaacatc tgctggcaga cgaaaattca
    14221 aaatatcatc atagccccat tcatcatatt gaatacttac tttcaagtca aggtatctac
    14281 cagcctgctg attatttttc aaagggatca gctcgacttt tgctgctttg ctgctaagcc
    14341 atttttgcgc gatgcgtgtt ttgatttgct tgtactctgc aacggcaacg tttcgttttt
    14401 cgggaaaaat aatcgtttga cgcaaaatct tttgcgttag catccattca taatcagtga
    14461 aaagattttt tactttctgc atcgcttcag atggcagatt tttctcgcca tctttccatt
    14521 tttgccattg ttctggactg atcagcaagt attcatctaa aaaatttttt tcagtttcaa
    14581 actgttctaa taatacactc ataataagat caatatatac ttgatgcacg tttatcccct
    14641 ccttttaact attctactta agttttgatc aaaaagcacc tcttaaatcg ctgctttcat
    14701 tttttttaat gtttcaagtc ctctcctctt tattcttcgt gatttttgtt ataataatag
    14761 acagacaaga aactcaatcc aactatgaag gagcgttttc agatgaaaaa atatcaatgg
    14821 ggaatcgttg gtcttggtac catcgcacat gagtttgccg aaagcttcaa tcaagagacg
    14881 agcgaactaa cagcagttgc ttccagaact tcagaaaaag cggaaaactt cgctcaccgt
    14941 tacaatattc caaaagctta cggaagctat caagaaatgt tagacgatgc tgaaatcgac
    15001 atcgtgtaca ttgctgtacc aaatagacag catatcgacc atatcttggc agcattggaa
    15061 gcaggcaaac acgttctatg tgaaaaagcc atcacgatga ataaaaaaga actggctgac
    15121 gctatgcgct tagcagaaga aaagaatttg atcttagcag aagcgatgac aatcttcaac
    15181 atgccacttt atcagcagtt acgctcaata atggatacag gaaaactggg ggctttaaaa
    15241 atgatccaag cacctttcgg cagttacaaa gaaccagacc ctaaaaaccg gttcttcaat
    15301 ccagaattag ctggaggagc tctgttggat atcggtacgt atgctgtatc tttcgctcgt
    15361 ttctttttga gctctcaacc tgaagtagtt gcctctacaa tggttccttt tgaaacaggc
    15421 gtggacgaac aatccgtcac tatcttgcgt aacaaagaaa atgaacttgc tgctgtcagc
    15481 ttgacttttc aggcaaaaat gccaaaagtc ggtatcgttg cctttgagaa cggttatgtg
    15541 acgattgctg attacccgcg tgctgatcgt gcggagatct tatttacaga tggtacaaag
    15601 gaattcatcg aaagcgggaa tacatctgaa gcaatgaatt acgaaataaa gaatatggta
    15661 cacatgattg aaggtgactt gcccaatcgc tctctcttct tgacaaaaga tgtcatcgaa
    15721 atcttggatc aaatgcaagt tctatggaaa aatatgtaat tttaaaaggt tgtgaaaaag
    15781 ctgttctgca tcaagtaata agaacagcca aacaaaaata gctcctccta tttttcgttt
    15841 ggctggactt attaccgcag aggaaagctt ttgaacaccg tttaagaaag aggttgtgag
    15901 cctaccgttc agctccgagt cacaaggagc aatttcaaaa aacagcttct catgtttgtt
    15961 gaatattgtg acttgtgacc gaggagttga caggagaaca ccgtttaaag aagaggttgt
    16021 gaaatcagct ttttgacctg aatattagct gaattcacaa cctctatttt tggatttttt
    16081 gttgggagaa ttctgccttg atttcaataa aacttttaaa agactgcaaa aactggaaca
    16141 attctgctcg atcctcgaat tcagggcgac tagtcggcag aggcatttgc cgatacaata
    16201 cataaacttc ttcaatacgt gttaaaattt gtctgccatc atttgactct gaaaatgttt
    16261 ctgcagtata aatcaataaa ccatagatat gttttccata ataagctggt tgctggattc
    16321 tctctagatt ttccagcatg tctttaatca cattggcctg tgctcttctc atagagaagt
    16381 aagtttcgta gtatagcggc tgtctgagcc agtagttttc ttgatgttct ctggcatctt
    16441 cttggctttc gcgaatgaat gtcagtaaat gttcgcattt gatctgcaca tcttctttat
    16501 tttctgataa aagaaattct gccatttgtc tcaaaatatt tcgaaattct ttttcgatct
    16561 gtatttggtt ttccttcagc tgcttcgtac gatctggcat gtaaacattt gcaagcaaag
    16621 caaatactaa tccaatcgcc attaatcccg cttcattgcc gatcagctgc caagacatgt
    16681 tttcctccac taaataatga gtgaccaaaa ccgaatttac tactatccct tcggttaatt
    16741 tgaagcgaga ggcaagcggg atgaacagga ataaaaaaag actgaagcca aaaatagtat
    16801 acccaacagc aaaaaaactg aacagtgctg ttgccgtagc taatataaag gctgcgattc
    16861 gattcatgcc cgtacgcaat gtagagcgct tggtatttcc tacacttaat aatgcaatga
    16921 tccctgcagc tggccaatac aacagcgata agctacccgc tgtgaccatc gaaaagaaga
    16981 cactgatcac tgttttgact gttcgcattc ctatacgcat ttacttcccc cttttttatt
    17041 ttaattatac ttgaataatg aaaaactagc tatcagacaa aagtttaggt atgatagtaa
    17101 aaagaccaaa aaggagaatc tctatgcaat ttggccgttt tcgactaggt atgcgtacga
    17161 tcaaaagtgc cttagctgtt tttttatgta tcttgttttt tcatttgact caacgaggaa
    17221 tcccgatgat tgctgcatta tctgctgttt tttctttgcg tcaggacttg actacaaccg
    17281 tttcttttgg aaagtctagg atcatcggca atacattagg tggtacatta gctatcattt
    17341 atttttttgt caaaagctat tttcacaatg attttttggt agaattgatc ttgcttccta
    17401 ttctagttat tattgtgatc gtcgtgtcag atggtattaa caataattcc ggtatcattt
    17461 ccgcaatagc tacgttgttg ctgattgcgt tgagcatccc tcaaggcgaa tccgctttgt
    17521 atgctatcca acgtgtacta gatactttta tagggacatt cattgcaatc ggtatcaatt
    17581 tctttcttcg tcctcctgaa acagaaaaga aggaagaact atcagaagat ttggtcgaac
    17641 tccaaaagaa agaacgattg ctaagaacaa atttacaaga gatagaagct aagatccaaa
    17701 aacaaaagaa aaatagcgag gactaatttt cttttgcaat aaaataaaaa aatggagaga
    17761 agaactttaa aaagtcttcc tccatttttg attgaacggt gaagtgtact actttattct
    17821 tccttcatcg agttttgttc tgcctctttt ggcgcttcat acatatttac ttggctagta
    17881 gtcccacgtg tcgacaaatt ctctacgatt tccttcaagt caagtcctgt tgtcgctttt
    17941 agtgtttctt gagtagaaga aagcaaattt gtggcataat ttgtcactcg gttcgctcca
    18001 ctggtttctc ctgaactgct tcctgtatcg acaacagaga ttttttcgat atttcctagc
    18061 ggctgtgctg cttcgcgcat caattgcggt agcatatcga taaccatact caagacagct
    18121 gcttcgccat attccttgaa ggcattggcg atcttttgtt ttgcttcggc ttctgctttt
    18181 ccttttgcta gtgctgcttc ggcttctgct tgtccagcta aacgtgtctt attggcttcc
    18241 gcttctgcca gtgcttccac acggaatcgt tctgcttcgg cttcagtcac ttctttgact
    18301 ttttgagctt gtgcttcttg ttctttagcg taccgatcgg catctgcttt tttcttcact
    18361 tccgaatcat attgcttttc acgacgtgtg atttcttttt cttccagttc gatttgtttt
    18421 tggcgttcta cgattttaat ctgcatttcc tgttcgatta cttgttgttg tgctcgggca
    18481 ctttctaaat tgtaggcttg gtctgctttt gctttagcaa catcttgttc ttgtttgtag
    18541 gttgctaatt tcagttcttt ttcttttaat gattctgcga tctctgtttg tcgctgtaat
    18601 tcagctgctt gtgattcttt ttctgcttga gcttttttga tacgcgtttc ttttagagct
    18661 tccgcttctg ctatatctgc atctcttttg acttgggcga ttcttggttt ccctaaagag
    18721 tcaagataac cgtttttgtc tctgacctct ttgatagtga aagaaacgat gatcaacccc
    18781 atttttgcaa ggtctacaga agcgacttct tgtacgcttt gactgaactt atcgcgattt
    18841 tgatagattt cttctacagt catagaacca agaatcgagc gcaagtgtcc ttctaatact
    18901 tctcttgctt cattttccag ttcttctctt gtcttgccta aaaactgttc tgctgctgta
    18961 gcaatctctt ctacagatga accgattttg atgatcgaag taccatcaca catcaccggt
    19021 acgccttgtt cggtatacac ttcaggggtg gagacgtcca atttactcga taatagactg
    19081 atccgattcg aacgttgaaa aactggcaag acgaatgcgc cacctccacg cacaattttg
    19141 attttgttat tgctttcatc aatgtgcaca tttttgcttc ctaaataact tccactgatg
    19201 atcaacgctt catccggttt tgctgtctgg tacttcgcta caaagacgat cagcaacatc
    19261 agtaaaatca aagctgcgat tgcgatcgcg ataaaaacaa ctggtatttc catttaacac
    19321 acgctccttt aagcttttat catgctttca taaggcatga cataacagat tctttccttg
    19381 atctcaatga tcaatacttt cgttccccgc tcagctgtct gttcttgggg gctgtaaaaa
    19441 caagctggac gactaatgga accggtaaca cttttcaact gaatttctcc catacctcgt
    19501 acagggatcg gtgtgatcac aacagctact tgtccttcca gagttttttc agaagacgac
    19561 atcgttgatt cggcatgttt catcggcatc aatatataga aattaagcaa aaataccaac
    19621 actatcgata tgcctccact agcaataaat aaaaataaag atgattgatc tgttaaacgc
    19681 tcacctagaa aaccaaataa gcttgtgaaa gttaaccaag ggataataag catcggatca
    19741 agcgggccat caaaatcgaa tacatctcca aaaataatca ataggactgc caatcctgca
    19801 caaatcagca atgtatacca ataaatcgtc tcaagtgcga ttccccctat catatacacg
    19861 cctccttcca tttctacgtc tatcatacca atgtttgaaa attctgtata gcttctttta
    19921 ccgactcagt ggtgtttttt ataggaaggt tgtgtttatc gttgacaatt tgttaaattt
    19981 ttgttttcat gttaaaattt tcacaataaa ttctttcgag gtagcctatg tttccttttt
    20041 ttaagaaaaa accattaaag ctccagaaca cgatcatcct tgtcgtttgt ttctccacat
    20101 tcgtttcttt gattgtttct gccatattga tcaggaattt tattctttcg atgaaccgat
    20161 ctagtgtgtc agataaaatc tctagcattg ctaaaatcgt tgccaatgat aaactcatcc
    20221 aaaaaaagct gcagactggt gacccgacac atgcgattca aggaataaca aatgaaatca
    20281 tgattgcatc gaatttagat tttatcgttg tcatggaccc tgaaggcatt cgatattctc
    20341 acgcagaccc aagttacatc ggtcttcctt tctcaagtca tgaagatgtg aaaaaagtga
    20401 ttggaggaca agaatttttc tctgaaaaag aaggaatgtt agggttcggc ttccgctatt
    20461 tcgtccctat ttattatgat caagagatta ttggtgcagt ttgtgttggc ttaacaaaca
    20521 acacgattga caatcaagtc aaagatggac aaaaaatgat tgttttttca ttgattattg
    20581 gacttagcgt aggcatcata ggcgccgttt ttcttgccca aaaaatgaaa aaagtactgc
    20641 ttggacttga accacaagaa atagcagaac agttaagtga aaaaacgatt atcgaaaatg
    20701 aagtagatga aggaatctta gccatctctt ctgataaaac tatcctcttg atcaatacac
    20761 ctgcaaaaaa cattttctac caaacagagc agctattgga tttcgaagta ggaaaaaaat
    20821 cagtgataat ctatatgacg ttttgtttaa agagacgatt gagcatcaaa cgaccgttcg
    20881 gactcaatcc atgtatttaa atgggataga aactatttct agcgttacac ctctttttag
    20941 caatgaccgt tttttaggag cagtcgcaac tttacgaaat cgatcagaag taatgaaatt
    21001 aatggaggag cttgggggga cgaaacaata tataaactcc atgagagctc aaacccatga
    21061 attcatgaat aaacttcatg tcatttctgg tttgattgat ttgaagaagt atgaagaagt
    21121 cacccatttt atccagcagc taaatcaaaa ttataaaaaa gagattggcc aagtctcaag
    21181 tctgatcaaa gtacctgcaa ttgccggttt tttattaggc aaaataaacg aagccaaaga
    21241 acaatctgtt gaaatgctgt tagaaagcga ttcttttatc cctaaaatgg agatggatga
    21301 ctccatccat tcacttttgc aagtgttagg gaacttactt gataatgcaa aagaagccgt
    21361 gctacaaaaa catgaaagaa acggacaggt ccggattcat ttaaactatg acacagaagc
    21421 tgaagtattc atcgtgaccg tctgcgatgg cggtgtcggg atatcagaag aaatctcaga
    21481 aaaaatattt acgctaggtt tttcgacgaa gggaatcaat cgtgggtttg gattgaatct
    21541 catccattca atcgtcaaac gccacggtgg atttttagaa ttttccaaac aaacaaatgg
    21601 cggtacaatc gtctatctag aattaccaaa aaaggaagat caaaatgatg acaaatgtcc
    21661 tgataatcga agatgatcca atggttgcag aattgaatcg tacttttgtg gaaagcatcc
    21721 ctaatatgaa cgtaatcgaa agtttcagaa atgtagaaga aggaaaaaaa tatttcagac
    21781 aaaaaaacgc agatctgctg ctgcttgatg tatacatgcc tggcgaaagt ggtatccatt
    21841 ttttatcttg gcttagacag caaggaaatg atatcccagt gattttgatc actgctgcag
    21901 atgatatcga aaccataaag aatgtgatcc gctacggtgc cttagattat ttgatcaaac
    21961 cgttcacttt gcaacgtttt cagctggcga tcgaaaagta tcttgctttc tctcagacaa
    22021 tccaaacgac tgaaaaagcc gatcagaaat tgatcgatca agtattcaac cacaccactc
    22081 ccattcacga aataccatca ttcatcgatt tgcctaaagg tctgtcaaaa ctgactttaa
    22141 aaaaaataag tgcatttatt ctaaaagaag atactatttt ttcaacaaaa gaactggctc
    22201 aaaaaagctc actgtcacgt atatccgtca agaaatattt gaatttttta gtagaacaag
    22261 atatattgaa cgaaagaatc aaatatttgg aaatcggcca gccgctaacc tgttatgaat
    22321 tgacaaagaa tgggcggaaa aagctcacag attactgttg agtcatcgaa ttatcccata
    22381 caaaatagtc caagtataca gaagtaacaa ctgtttactt ggactatttt taaacatttt
    22441 attgacgaat atccatgttt tcttatctta attgacgggc tttaggaaga gatacctcat
    22501 ttttcgtttt tccagtttct attatttcac gaaagttctc gaaacttgtc gaaatcatat
    22561 ttgataatgc ctcatctgta tttgaaccta catgcggggt tacaagaact ctagggtaca
    22621 tttctactaa ttgctggata gcagggtctg gaatcgtctc ccatggcttg aaggatcgga
    22681 aaaaaacctc tgtttcattt gcaaaaacat cggttgcaaa accagctaaa tgattgcttt
    22741 tcaaagctct taaaatagct tcattatctt gaagctctcc acgggctgta ttaatcaaga
    22801 tgctaccttt tttcatttga gctaaaaatc cttcattgat catttgatcg ttttcacctg
    22861 ggatatgagg tacatgaata ctgacaatat cgcttttggc taataactct tcaagcgagt
    22921 ggaacgtcaa tactttttta gcagcttctg tctgatgaat atcgtagccg atcactttgg
    22981 ctcctagtcc cttaaacaaa acagcttccg tcaaccctat cctgcctgtt cctatgatcc
    23041 ctactgtgca atttctgatt tcacgactaa acatttgctc atccactcga aaatctttga
    23101 aagatgttcg gatcgtcgta tacgcagtat ttctcaaaag catcatggca aaagtcaaag
    23161 ataactctgc tatcgcattt ggcgaatacg atggtacacg ggctactgtc ataccaaaat
    23221 cagctgccgc ttgtagatcg atatgattga ctcccacagt ccgtgtaaaa acataacgaa
    23281 tcccatattc ctgcattttt gcaagattgg tctcatctgc tatacaattt cccctcaaca
    23341 atacagcatc atggtccttt gctgacacga tattgtcatg tgtcaacagt tcttcataaa
    23401 gactgagatc ataatggtaa atattcaatt tttcaaaaaa atctatctcg ttcggacgaa
    23461 caccgtaaca tgcgattctc atactttctt cctccatttc ttatgaaaat aaacctatgc
    23521 catgaatcat ttgtaataca acgatccata caggtgtcag taccacagca aaaatagttg
    23581 acagtaagga tgcattggag gctaaaagtg cctccctatc aaaactgata gcataggctg
    23641 ctgccactgt tgcagctggt gtagccatca tgataacgat tgttgccagt gcttcaaaat
    23701 tgactggtag aatattcgtt gccgtcaaaa ctgctagaat cacgatattc aatgcaggta
    23761 caaataccac tttgactaga ctgtaatacc atgaagtttt ttcagatgca gcttttttga
    23821 agcttacttc acctaatgtt gcgccgattg ctagccacgc caatggtgaa cttaaaccag
    23881 ctaaataatc catcggctga aatagccacg gtgcagtctg gtcaattcgc aaaaacgcta
    23941 cttggcttat ctctccttct gcacctgtaa ctgacacttg tggcagatac ccttggaaca
    24001 gccaaatgaa aaggccggca aaagtagcga taatgattgg attgacgaac atcgctttga
    24061 tatttttagc ttccatcttc aagccactca tttttatgta tccataagaa tacaaaaaga
    24121 ttcggtagcc aatattgaaa attgaggaat acatcacgcc gacaggacca taaatcgcac
    24181 tgacgatagg cgttccgaaa aaggtcgtag aaccaaagat cgtcaggaca cgcaacgttg
    24241 tttgcttatc ccctttatag cttaagaaaa aaggttttga aactagaata agaataatat
    24301 aaataactaa tccccaaatc aagacattga ttccttgcat cagtaattct gtattgatat
    24361 cttgcatgaa agctcgaaat gccagtgctg gcaaagccac tgttaagacg actttcgata
    24421 gaatttttcc aacagattca gaaaaaatcc ccttttttct acaaacaaac cctaataata
    24481 taataaaaac tgttgaagtg attgcactga caatcgacat atctgtcaaa gtcgatcgaa
    24541 aaatgtcaaa taagttcatc tttctcctcc taattatgtt catttattca caataaaaaa
    24601 agcttttat
//
LOCUS       DAPCMT010000028        23831 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPCMT010000028 DAPCMT010000000
VERSION     DAPCMT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.028"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(55..789)
                     /locus_tag="RD743_000955"
     CDS             complement(55..789)
                     /locus_tag="RD743_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDU0562783.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="RD743_000956"
     CDS             1116..1526
                     /locus_tag="RD743_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDU0562784.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="RD743_000957"
     CDS             1519..2220
                     /locus_tag="RD743_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDU0562785.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="RD743_000958"
     CDS             2220..3806
                     /locus_tag="RD743_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0562786.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="RD743_000959"
     CDS             3825..4046
                     /locus_tag="RD743_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDU0562787.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="RD743_000960"
     CDS             4043..4804
                     /locus_tag="RD743_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDU0562788.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="RD743_000961"
     CDS             5184..5693
                     /locus_tag="RD743_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDU0562789.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="RD743_000962"
     CDS             complement(5768..6307)
                     /locus_tag="RD743_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDU0562790.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="RD743_000963"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="RD743_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDU0562791.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="RD743_000964"
     CDS             7320..8171
                     /locus_tag="RD743_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDU0562792.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKTSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="RD743_000965"
     CDS             8199..8834
                     /locus_tag="RD743_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDU0562793.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="RD743_000966"
     CDS             8854..9627
                     /locus_tag="RD743_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0562794.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="RD743_000967"
     CDS             10035..10832
                     /locus_tag="RD743_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDU0562795.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="RD743_000968"
     CDS             10810..11223
                     /locus_tag="RD743_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDU0562796.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="RD743_000969"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="RD743_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDU0562797.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="RD743_000970"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="RD743_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDU0562798.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="RD743_000971"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="RD743_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDU0562799.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="RD743_000972"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="RD743_000972"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDU0562800.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="RD743_000973"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="RD743_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDU0562801.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="RD743_000974"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="RD743_000974"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDU0562802.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="RD743_000975"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="RD743_000975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDU0562803.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="RD743_000976"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="RD743_000976"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDU0562804.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="RD743_000977"
     CDS             21907..22485
                     /locus_tag="RD743_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0562805.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="RD743_000978"
     CDS             22537..23019
                     /locus_tag="RD743_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDU0562806.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="RD743_000979"
     CDS             23136..23510
                     /locus_tag="RD743_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562807.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7784 a         4284 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaacttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DAPCMT010000029        23383 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPCMT010000029 DAPCMT010000000
VERSION     DAPCMT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.029"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            12..1673
                     /locus_tag="RD743_000980"
     CDS             12..1673
                     /locus_tag="RD743_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0562808.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1743..2090)
                     /locus_tag="RD743_000981"
     CDS             complement(1743..2090)
                     /locus_tag="RD743_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDU0562809.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2174..3115
                     /locus_tag="RD743_000982"
     CDS             2174..3115
                     /locus_tag="RD743_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562810.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3116..4159
                     /locus_tag="RD743_000983"
     CDS             3116..4159
                     /locus_tag="RD743_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562811.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4165..5235
                     /locus_tag="RD743_000984"
     CDS             4165..5235
                     /locus_tag="RD743_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562812.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5225..6178
                     /locus_tag="RD743_000985"
     CDS             5225..6178
                     /locus_tag="RD743_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0562813.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6190..7200
                     /locus_tag="RD743_000986"
     CDS             6190..7200
                     /locus_tag="RD743_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0562814.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7485..8306)
                     /locus_tag="RD743_000987"
     CDS             complement(7485..8306)
                     /locus_tag="RD743_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDU0562815.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8403..8681)
                     /locus_tag="RD743_000988"
     CDS             complement(8403..8681)
                     /locus_tag="RD743_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562816.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8949..10958
                     /gene="metG"
                     /locus_tag="RD743_000989"
     CDS             8949..10958
                     /gene="metG"
                     /locus_tag="RD743_000989"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDU0562817.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11437..12024)
                     /locus_tag="RD743_000990"
     CDS             complement(11437..12024)
                     /locus_tag="RD743_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562818.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12199..13521
                     /locus_tag="RD743_000991"
     CDS             12199..13521
                     /locus_tag="RD743_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0562819.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13676..14452
                     /locus_tag="RD743_000992"
     CDS             13676..14452
                     /locus_tag="RD743_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDU0562820.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14445..15014
                     /gene="rnmV"
                     /locus_tag="RD743_000993"
     CDS             14445..15014
                     /gene="rnmV"
                     /locus_tag="RD743_000993"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDU0562821.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15007..15891
                     /gene="rsmA"
                     /locus_tag="RD743_000994"
     CDS             15007..15891
                     /gene="rsmA"
                     /locus_tag="RD743_000994"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDU0562822.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16342..16971
                     /locus_tag="RD743_000995"
     CDS             16342..16971
                     /locus_tag="RD743_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562823.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17095..18204)
                     /gene="ugpC"
                     /locus_tag="RD743_000996"
     CDS             complement(17095..18204)
                     /gene="ugpC"
                     /locus_tag="RD743_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDU0562824.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18413..18835
                     /gene="mgsA"
                     /locus_tag="RD743_000997"
     CDS             18413..18835
                     /gene="mgsA"
                     /locus_tag="RD743_000997"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDU0562825.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19158..19694
                     /locus_tag="RD743_000998"
     CDS             19158..19694
                     /locus_tag="RD743_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDU0562826.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19734..21497)
                     /locus_tag="RD743_000999"
     CDS             complement(19734..21497)
                     /locus_tag="RD743_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562827.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21500..23215)
                     /locus_tag="RD743_001000"
     CDS             complement(21500..23215)
                     /locus_tag="RD743_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562828.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7525 a         4285 c         4767 g         6806 t
ORIGIN      
        1 gggtacgtaa aatgaaaaag aaatttacat ttggtttggt tgcggtatgc ggccttgtac
       61 ttgctggatg ttatggcggc agttcagcca caagcagtag ttcagcaaaa agcggttcag
      121 cagatggagg aggcgtattt aacttagtcg tgcctcaaga aatgcctaca gctgacttat
      181 cagttgcaac agatacaatc agttttacag cattgaataa cgtttatgaa ggaatttacc
      241 gtcttgatga agacagcaaa ccacagccgg caggagctag cgagttggca gaagtcagtg
      301 aagacggatt aacttacaaa atcaaattac gagaagacgc aaaatggtca aatggtgatc
      361 ctgttacagc agcggattac gtttatggct ggcaacgaac agtagaccca gctacagctt
      421 ctgaatatgc ttacctgtat gcgccagttg aaaatgcaga agacattaca gctggaaaga
      481 aagataaatc agaactaggg atcaaagcag ttggtgacta tgaattagaa atcaaattga
      541 cgaaacaaac accatatttc caatatctgc ttgctttccc atcatttttc ccacaaaaac
      601 aatccgtagt ggaagaaaat ggggatgcct atgcatctgc aagtgataaa gctgtttata
      661 atggaccatt cacattagaa gattttgatg gaccaggaac agatacagaa tggacttata
      721 agaaaaatga tcattactgg gacaaagata cagtaaaact ttctgaaatc aaagtcagtg
      781 tagtaaaaga atcttctact gcattgaact tattcaaaga tggccaagca gatgacgtga
      841 tcttgtctgg tgagttagca cagcaaaacg caaacgatcc agcttatact tctgttaaag
      901 aagcgcggac aagctatatc gaattcaatc aacgtgaaaa gaattcacca ttcaacaatg
      961 tgaacctaag aaaagctatt tcttattcta tcgatcgtga agctttagta aaacaagtac
     1021 taggtgacgg atcagttgtc tctacaggat tgatcccatc agatatggta aaaagtccaa
     1081 caacgaacga agactttgca aaagaagctg gaaaattagt ttcttatgac aaagataaag
     1141 caaaagaata ttgggaaaaa gcgaaaaaag aattgggcgt tgattcttta gaatttgatt
     1201 tgatggcttc tgatgatgat tcatcgaaaa aagtaatcga atatgttcaa aactcaatcc
     1261 aagaaaactt ggatggcgta acagtcaaac caacacctgt tccattctct gtacgtcttg
     1321 accgctcaac ttcgggtgat tttgacgcag tactaggcgg atgggctgct gactatgcag
     1381 acccaagcag ctttactgat ttgtttgtca caggtaattc atacaaccgt ggtcgctgga
     1441 gcaatgcaga atatgacgaa gcagttaaag cagcttcatc aacagacgct ggcgatgaac
     1501 aagcccgttg ggatgatttc caaaaagcaa tcaagatcat ctctgatgat atgggtgtcg
     1561 ttccggtgta ccaaaaagca gaaggtcacc tagtgaatga aaaaatcaaa ggtatcgttc
     1621 accatgcggc aggcgcttca tgggattaca aatggacata cgttgaagaa taatcgttaa
     1681 taccgtgagc atgagaaaaa acgaggctgc ggcaaatgtc gcaacctcgt tctcatattc
     1741 acttatccta acaaagaaaa aagcatgaaa atagcagaaa gagcaattag tattccggca
     1801 atgatcagcc aaaagctgta cataccgtag ctggcagacg agagcgagga aaattctggt
     1861 tttttctttc ggttccttgt tcgtgcaaga tggaccgatc gctgaaaatg atcgaagaaa
     1921 cccgaagaaa agacccataa aaaaccgccg ataatcagaa acggcaatga ccataagaat
     1981 agatcattag ataattgaat gagtgtaaag gaatggttga tgagattttt tattaaaata
     2041 accagaatca gtagaaaagc tactgcgtag gggatagatt tttttttcat ttgattgctc
     2101 ctttattttt ggttcattat ctaataatgt agcgaaacat ctgtcagaag tcaaaagcta
     2161 gggggaaaca ttagtgaata gttatatcaa atatgtatta aaacgtgtct ttttcatgat
     2221 catcacgctt tggttaattg caacaatcac ctttttcttg atgcaattgc tgccgggcac
     2281 gccatatacg aatcaggaac gtttgagtcc tgaaacaatc gagatgctaa ataaacaagt
     2341 cggtttggat aaaccagtta tcgtacaata tgggatttat ctttctaatt tgcttcaagg
     2401 agattttgga atttccttcc aatttaaaaa ccagccagtc gcacatttgt tagctggaag
     2461 aatcggacca tccttgcagt tagggttaca ggcaattatc tttggaacag tcctagggac
     2521 gattttagga acgatctctg ccatgaagca aaatacatgg gcagatacat cttctacact
     2581 cgtagcgata ttaggtcgtt ccattccaaa ctttgtattt gctgttttat tacaatatat
     2641 ctttgcaatc aaattacaag ttttgccaat cgcaaaatgg gatggctttg tgtacacaat
     2701 cttaccaaca attgcgttgg ccatgtcgcc gcttgctgac tctgctcggt ttatccgtac
     2761 ggaaatggtg gaagtactgc atagtgatta tgtggaatta gcaaaagcaa aaggattgag
     2821 ccgttgggaa atcgccttta aacatggtct tcgaaatagt ttgattccat tgatgacttt
     2881 attaggaccg ttagctgtag cattgatgac cggatcactg gtggtagaaa atatctttgc
     2941 tattcctggt atcggggaac aattcgttaa gtcaatcaca acaaatgatt atcctacgat
     3001 catggctgta acgatccttt actcatttat gctgattttt gttattttga tcgtggatct
     3061 attgtacggt ctagttgatc caagaattcg tgtttcagaa gggagccgag gataaatgga
     3121 aatgatacca aagcgttatg aaacagttgc agatatccct gcggatgaat ttcttccatt
     3181 acaaaaaaat accgaagaag aacgtgaaca gattactgca ccttctttga attttatcca
     3241 agattcatgg cgccgtttaa agaaaaacaa agctgccgtt atttcaatgg ttttactatt
     3301 ggtcatcatt ttgatctctg tgatcacaat ctttgtttct cctcatgatc caactgccca
     3361 aaacgtggct tatatcaatt tgcctcctcg tattccggga atcaatatcg atggattgaa
     3421 tggaaaagca atggttggtg gtgaacttgt tgataaatat gctcaagcaa acgtcccttc
     3481 aaacgtaaat ttctttttag gaacagacgg tttgggacgt gatgtattaa gccgcttatt
     3541 tatgggaatg agaatctcgt tgctgatcgc atttattgct gcattactag atgtaacgat
     3601 tggtgtagct tacgggttgg tttctggttt gttaggcgga cgtgtcgata acgtgatgca
     3661 acgttttttg gaaatcttat caggaattcc aaacctcgtt gtcatgattt tgatgttggt
     3721 cgtttttgaa ccaggaattt tttcaatcgt ggctgctatg gccatcacaa actggatacc
     3781 aatggcacgt atcgttcgtg cacaaacaat gaaattaaaa gatcaagaat atgttttagc
     3841 tggattgaca ttaggtgaat caagaatgaa aattgcattc aaacatattt tgccgaatat
     3901 ctctagtgtc attattattc aaatgatgtt tagtattcca tctgctattt tcttcgaagc
     3961 ctttttaagc tttatcggtt taggtcttac gcctccgtca gcttcattag gaacgatgtt
     4021 gagcgaaggg tacaagacat tcctgtatct gccttatcta ttatggattc cggcagtaac
     4081 tttatcggtt atcatgattg gctttaactt acttgctgat ggattgcgtg atgcctttga
     4141 tcctaaaatg aaagagtgaa gacgatgacg aaaattttag aagtgaaaga cttagaaatc
     4201 tcttttgata cgtatgctgg aaaggtacga gcgattcgcg gtgtcaattt tcatttgaat
     4261 aaaggagaaa cattggcgat cgttggggaa tctggaagtg gaaaatcagt gacgaccaga
     4321 agcatcatgc gcttgctgtc aagcaacgcc aatatagatg ctggtcaaat cctctttaaa
     4381 ggtaacgata tcgttggaaa atcagaaaga gagatgcaaa aaatccgtgg taaagaaata
     4441 gcgatgattt tccaagatcc aatgacctct ttagatccga cgatgcccat tgggaagcaa
     4501 gtcgctgagt cccttagaaa gcataataaa gtttcaaaaa aagagggaca aaaagctgca
     4561 ttagaattgc ttaaattagt tggaataccc gaagctgaga aacgtatcaa cagttatccg
     4621 catcagttct caggaggtca gcgtcaacgg atcgtgatcg cgattgcact ggtatgttat
     4681 ccggaaatcc tgattgctga tgaacctaca acagcgcttg atgtgactat tcaagcacag
     4741 attttggagc tgctgaaaga aattcagaca aaaatcgata catcaattat ctttatcact
     4801 catgaccttg gtgtagtagc gaatgtcgct gatcgtgtcg ctgtcatgta tggaggaaga
     4861 atcgttgaag ttggaacatc ggaagaaatt ttctacaacc cgcaacatcc ctatacttgg
     4921 ggactgcttg gttcaatgcc gacattagat agtgcgaacg atcgattgta tgctattcct
     4981 ggttctccac cggatttatt gaacccgcca aaaggagacg cattctatcc aagaaatgaa
     5041 ttcgcgatga aaatcgatgc agaacaagaa ccaccatttt ttgaactatc aaaaacacac
     5101 aaggcagcaa cttggctact tgctccgcag gcacccaaag tgacaccgcc agaagaaatc
     5161 caacgtcgct gggctatctt taaagagaaa caaaaacata cttacggtca aggaggaatt
     5221 gctgatgcca aataaactac ttgaagttaa aggattgaaa cagtatttca atgtaggaaa
     5281 aaagaatgaa gtacatgctg taaatgacat cagctttcac atttatgaag gagaaacatt
     5341 cggtttagtt ggagaatctg gaagcggaaa atccactact ggccgtacga tcattcgctt
     5401 gaatgaacct actgatggtg agatattatt cgacggtcaa gatataacga agatcaaaga
     5461 taaaaaaggt ttgacgaaat tccggcatga tgttcagatg atctttcaag atccatacgc
     5521 ttcgttgaat cctcgtatga aagtcagaga tatcattgcc gaaggaattg atgttaatgg
     5581 actagctaaa tcatctgaag agcgtgcaaa aaaagtcgat gatctattga gaactgttgg
     5641 attgaatcca agccacggta ctcgttatcc tcatgaattt tcaggtgggc aacgccagcg
     5701 tattggtatt gcacgagcat tggcagtaaa accgcgtttt atcatttgtg atgaaccaat
     5761 ttctgcacta gatgtgtcga tccaagccca agtggtcaat ttgttgcaag atctccaaaa
     5821 agaacatcaa ttgacctatc tatttattgc acatgatttg tcgatggtta aacatattag
     5881 tgaccgaatc ggtgtaatgc ataacggctt gttattagag atgggaacaa gtgatgaaat
     5941 ctataatcac ggtgttcatc cttatacaga aagtttgtta tctgctattc ctctaccaga
     6001 tccagaccat gagagaaaac gcagaagaat caaatatcaa cctgaaccag atgacggaca
     6061 agtacgtcaa cttcgtgaaa tcgcaccaga acattttgtc tatgcaactg agcaggaagt
     6121 cccatattac gcaaaaaaac taaaacgaca aaaggaatct cttctggttg ctaactagga
     6181 gggacataga tgctaaaagc ttttaaattc cgtatgtatc cgacagaaga acaaaaacag
     6241 caattgattc gtacttttgg atgtgctcga tttacctata accatttgct gaaaaagcgg
     6301 cagaaaagtt ggcaacaaac aggagtagcc aatttttcgc tcacacccgc tacattaaaa
     6361 aaagaatacc cattcttaaa agaagtagac agtcttgctt tagcaaatgc acagcttaat
     6421 ttggatcggg cttttcgcaa ttatttcaag ggacgtgcca gcttcccaaa gcttaaaacg
     6481 aaaaaaagta tgtggcaatc ttatacgacc aacaaccaaa cccgtacgat ctatctggaa
     6541 aatggctact taaagttacc aaaacaaaaa gaactaatca agataaatca gcatcggcca
     6601 gttgaaggat cgattcgctc cgctacgatt tctgctcgat ataatgaaga attttatgtc
     6661 gcactacttt gtgatgtttc gccagtaaaa aaagaatctt tagctaaatg gatcgggatc
     6721 gcctatcatc cgaagacatt gatccaaaca tctcgacctc tagaagtaac tttaccaaaa
     6781 tttcaccaga cagaagaaaa gctgcaacat gcacagcgga aattgaatgt gaaagtccgc
     6841 tccgctcacc atagaaagat caggttagat caggcaagca attatcagaa acaaaagaga
     6901 aaagtaatgg atctttatct gaaacaaaag aaccagcgag aagattatct tgaacaatta
     6961 tctgggaaat tggtcaaaca atatgactac ttatttgtag aatcttttcc aaaagaagag
     7021 gcacatgctg atttttctat tcacgactgg cataaattaa taacaaaatt gcgttataag
     7081 tcccaatggt ataataaaaa atttcttttg atcaataccg acggagcaga agaaagcaat
     7141 tccgtaagaa agagccaagt agtagaaaaa atgggcagac attcggttat caagggataa
     7201 aaaatgaatg aaactgatag gagcgttagg gttagccttg ttaataaaaa gtcttaatga
     7261 cttttgtttc taggaatttt tcttttgaag aaaagctcac taattatgac aaaccaataa
     7321 aacaatagca gaaaaagttc gtacgagctt tttctgctat tgttaatttt ttattcagaa
     7381 agtttacgaa tacatcaaaa ttctgaaaca aaaaacgtaa ctcacaaaga gttgtttttg
     7441 cgttttttct tgaatgatta ggtctgaatc ttttgctcac aaccttattc atggtgcccc
     7501 ggcttgagct gatagatttt tgaaggacgg ccgattccta caggacgttc accgatctca
     7561 tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat cgatcgtttt ataatcgatc
     7621 cctaagaatt tagcaaatac ttttcgagct tccgtaatcg tgaaatcttt acctaaaact
     7681 tgtagaactt gcggctcgtg ttccatttta ttgactacac ggttgaatgc tttcaagatg
     7741 atttgactat ggtcaaaagc caaagtatct ttgccagaag actctccagt gactaaatca
     7801 agcgagatag ccacttctcc gctagataaa tggatcttgt tactatgacg ttctagtgtg
     7861 aaccagcgta cttccttggc atcatctccg gcgattagcg gttcttctcc aataaaggct
     7921 aagtagctga ctgtgacgac ccagcctcgc ggatctcgat taggcgtact gaatgtatgc
     7981 agttgctcaa tattttcttt tgaaataacc acaccagttt cttcctttgt ttctcttaag
     8041 acgctttctc cagttgactc atccttttgg acaaagccac caggcaatgc ccatgaattt
     8101 cgataaggat gcccttttct ttggatcaac aaaactttca gctgatcttc ttctttgtta
     8161 tagcacatca gtacgatatc gacagtcaaa gagggcttgt catattctgg acgttcctgt
     8221 ttttgatacc agttaagaaa ttcttcttca cttgctttat gttcataata taatttttct
     8281 tcttctttag aagcaaaacg gttcatctga ccacctccat tgacagtaaa agtacttttt
     8341 accttattgt agcagactct gaaaaaaaac caaggattta cttgaaaaat ctaggttatc
     8401 atttaaatat gaaaaataaa tggaagtatg agattgaata aaaagaaaag cagataaaac
     8461 ggcaatactt tataccgctc ttcacgaatg gcaaagaagc cacaaataac gatatatacg
     8521 tcaatgataa aacgcaaaat ccaagctgct gtcataaaac tgttcaaatg cccatggagg
     8581 ttaaaataac catacatacg aatataaaaa tagctgaata ttaaatgtgc taatgaaaga
     8641 aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca tgattgttcc agtcccttac
     8701 taagatagat agtctttatt ataccacgtg tgaggggaga aataaaaagc cgttttttag
     8761 tagatatgaa acaaaacaaa aacccgcatt tattcaacct attcatcctt ttaaaggata
     8821 gattgaacgt agatagaagt cgtttatcca gtctttttca ttttgaaaaa aagctaacag
     8881 catgcttttt ttatggtata ctagcaagga aacgaattcg catcatgcga taaaggagga
     8941 agagccccgt ggcagaaaag gaaacttttt atatcactac gccaatctat tatccaagcg
     9001 gcaaacttca tatcggcaac tcgtatacta cgattgcatg tgacgcaatt gctcgttata
     9061 aacggttgat gggctttgat gtgttttatc tcactggagt ggatgaacat ggtcaaaaaa
     9121 tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga atatgtagat aaaatggcgg
     9181 ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta tgataaattc attcgcacga
     9241 cagatgatta ccataaagca gctgttcaaa aaatatttga tcgtttatta gaacagggag
     9301 atatttactt aggtgaatat gaaggctggt actctgtttc tgacgaagaa ttctttacag
     9361 aaacacaact tgcagaagtg tacaaagatg acgaaggtaa cgtgatcggt ggtaaagcgc
     9421 caagtggaca cgaagtagaa ttggtaaaag aagaatcata cttcttccgc atgagcaaat
     9481 acgcagaccg tctattggaa tactatgaag aacatcccga attcatccag ccagaatctc
     9541 gtaaaaacga aatgatcaac aactttatca aaccaggatt agaggatctt gcagtttcac
     9601 gaacaacttt tacttgggga gtaccagtca agagtgatcc gaaacatgtg gtgtatgtat
     9661 ggatcgatgc attatccaat tatattactg cactaggata tggctccgaa gatgaaagtc
     9721 tattcgaaaa atactggcct gcagatgttc agatggtcgg taaagaaatc gttcgctttc
     9781 atacgatcta ttggccaatc atgctgatgg cccttgattt gcctctacct aaaaaagtat
     9841 ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc taaatcaaaa ggaaatgtcg
     9901 tttatcctga aatgcttgtg gaacgttatg gtttagatgc actccgttat tatttgcttc
     9961 gtgctgttcc gtttgggtca gatggtgtct ttacgccaga agattttgtt tcccgtgtca
    10021 attatgatct ggcaaatgat ctagggaact tattgaatcg aacgattgct atgatcaaca
    10081 aatattgtga tggactcgtt cctgactatg cctcacttgt cactccattt gatagtgaac
    10141 tgtctacgac ggctgctaac gttgtcggac gttaccatga tgcaatggaa aaaatggaat
    10201 tcaatacagc attggctgaa atctgggtgc tgatttctcg tgcaaacaaa tacattgatg
    10261 aaacagaacc atgggtatta gcaaaagacg aagaaaagaa agctgaattg aatagtgtca
    10321 tgatccattt agctgaatct ttgcgtatcg cagcgatatt acttcagcca atcatgacag
    10381 aaacgccaga gaagatcttc aatcaactag gattggactc tgaaacaatg aatcttgaag
    10441 gattacactt tggcgaattt ccttcaggaa caaaagtagt tgcaaaaggt acaccaatct
    10501 tcccacgttt agatatggaa gaagaagttg cttttattca agaaaaaatg tctgaaggta
    10561 ctcaaacaaa cgaagataca gtcaaatggg atccagaaga aacagaactt gtttcaacga
    10621 aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga actaaaagta gcagaagtca
    10681 tcaactgtca aaaagtagaa ggggcagata agttgctgca attccgacta gatgctggag
    10741 acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt ttatcctgat ccaagcgaat
    10801 tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc acgaaagatg cgtggacaaa
    10861 tcagtcaagg aatgatcctt tctgcagaag caccagatgg ctctttacaa gtaatcgaag
    10921 caccaaaatc catgccaaat ggctcagaaa tcgcatagaa attcaaaggg ggtatgacac
    10981 tagttgtgtc ataccccctt tttccgaaat ttgagaagca attttcgtaa attccttctt
    11041 attacttgaa gccaatcata taatacagaa acagagaact atccaaaata aatcctagaa
    11101 aaagaagtac gagcttcgct gatttttgtt aatttagaat aatactaata ggaatcgtaa
    11161 aaacagaaaa aaagcataat gataaagggt cgggcagatt ttaattgatt catctggtac
    11221 tcttttttat tgtccttaca ttcataaagt ttgaatgcaa tgactttatt acatctatat
    11281 ttatatctaa cagtagcgtt gatgtgttaa ttataagttc tatcacttta tgttcaatta
    11341 gtttgtgctc ttatatgtgt gattgaaaat atagataaaa aaacaatcat cggctttgac
    11401 ttcgatgatt gttttcttta ttttgtaaaa atcctatcac ttgattttat ggtaaccttt
    11461 tatttttgtc ttcaaacggc ttggtaagag agcagtaagc agtgcaaatg ggatcgaaaa
    11521 gacataccag aaaatcgtac gtccgatacc ataatgttga tagagctttg tcaacatcac
    11581 gtgtaacaag atcaacccta aaagcaagga gccagctata aaccaatgat tatcccaatg
    11641 catgacttca ctaagtgcca tcactccaaa aggatagaaa taaaaataga tcccgactaa
    11701 ggtcatccgc cagttctttg taaatgttcc gatattcaat aaaaagaaaa tcgctgaaag
    11761 caaaataccg ataggcggaa aaagcccaac caaaaaggaa tacaacatcc cccacaagat
    11821 catcacagga cctttgatca atgcaacgat tgcgatgaac aaagtcagca atattagttt
    11881 ttgtgataac gttgcaacag tcattaatcc aaataaaatt aatgtgaacc aatgccaatg
    11941 ggactgctgt tgttctttac gggaaaaatc gacccggttg gataaataat ctgctaattt
    12001 cggataagaa cgcttaaaat ccataaactg ttcctccttc gtggaaaagg taggtttagt
    12061 atacgaaaaa taaaaaagaa atacatccgt cttaagaggg agtttttcac tgaaagtgga
    12121 gaaaaaagaa tggtaaatta aaatattctc agttatactg aaaattatac caaatttcaa
    12181 agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag aaatcaatct gtttggcgca
    12241 ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct tcttcaaaac tgctgctgtt
    12301 actgctagta ctcagtctac aagtttgaca ttgctagctt ggcttttggg tggtttctta
    12361 acgatctgtg ctggattgac agtggctgaa ttagctacag caatcccgga aactggtgga
    12421 gctgtaaaat atattgaagc tgcgtatgga aaattgccca gctttttatt aggatgggcg
    12481 caaagcttga tttactttcc tgctaatatt gctgcattat cgattatctt tgcgacacaa
    12541 ttgacgaact tattacaatt atctaccgat tatcttttgt tgattgcagt gatcaccgct
    12601 gtttctgtga cagggttgaa tttgttaggg acaaaagtcg gtgcttctgt acaatcagtg
    12661 acgttgattg ttaaattaat accaattgct gtgattgtca tatggggatt attgacccca
    12721 ggacaaggaa ctgtccagtt atttccaatc gaagctggaa aagatgttac ttttgtcgaa
    12781 ggattgagca gtgcgttgtt agcaacgttg tttgcttatg acggctggtt aggtgtcggt
    12841 gcaatggcag gggaaatgaa acggccggaa aaagatttgc ctaaagcaat tattcttggt
    12901 ttgagttttg taacagtcgt ttatctattg atcaattttg tttttttgaa aactttgccg
    12961 attgatcatc ttgccggtaa tttgaatgca gcttctgaag catcagacgt catttttgga
    13021 ggaatcggtg gaaagctggt cactattggg atcttgatct cggtatacgg tgcattgaac
    13081 ggttatacac taactgggat ccgcgtgcct tatgcgatgg ctttagaaga cgagctacct
    13141 ttcagtaaac agctgacaaa gctttcaaag aagtttacgg ttccttatgt tcctgcggtt
    13201 ttccagttag cagtagcttg tattatgatg agccttggtt ctttcgactt tttaacagac
    13261 atgttgattt ttgtgatgtg gctattcagt ttactgattt ttatcggtgt ctttatttta
    13321 agaaaaaaag cgccggaatt gccacgtcca tataaagtac cactttatcc aatcgttccg
    13381 attattgcga ttctcggtgc tattttcatt ttaggtatga cgatgatgac gcaaacgaaa
    13441 ttagcattga tcggtatagg tgttaccttg ataggtatcc cagtctatta tcaaaaaaag
    13501 aaaaaacgct cagaagagta aaatatttca acaacagaca gaaaaagtac gaactaggtc
    13561 ctctagatct tcgtgctttt tctatttcaa taaaacaagt tcttctattt tgatttttgc
    13621 tacttctgca agtagtgctt atgtgctaaa gtaagaatga aaaggagaga taaacatgat
    13681 atttgattct catacccatt taaatgctga acagttcaat gaagatattc cagaaacgat
    13741 tcagcgagca caagaaatgg gggtaacgaa aatggcggtc gtgggcttcg atacgccaac
    13801 gattgaaaag tctttgcaat taagccatga ctatccaaat atatacagta ttatcggctg
    13861 gcatccgaca gaggctggca gttatacgaa agaaatcgaa aataaattac aagaacaatt
    13921 gactttacca aaggttgtag cattagggga aattggcttg gattatcatt ggatggaaga
    13981 cccgaaagaa gtccaagata aagtatttcg cagacagatt gctatcgcaa aagaaatgaa
    14041 cctaccaatc agtattcata ctagagaagc acttgaagac acgtatcaaa tcctaaaaga
    14101 agaagatgtt cgagatatcg gtggaatcat gcatagtttc agtggtgatt atgaatgggc
    14161 aaaacgattt cttgacttag ggatgcatat ttcatttagc ggtgtcgtca ctttcaaaaa
    14221 agcattagac gtccaagagg ctgcagcaaa tgtaccaatc gaacgattac ttgtggaaac
    14281 agatgcccct tatctggcac cagttccttt tagaggaaag cgaaatgaac caggctatac
    14341 tcgttataca gtagaaaaga ttgctgaact tcggaatctt ccgacagaag aagtggctgc
    14401 ccaaacatgg acaaatgcgc accgattatt tggtctaagc gaacatggat aagatgaaga
    14461 tccaagagat tatagtcgtt gaaggaaaag acgatacaag aaggctgcgg gaagtagttg
    14521 atgcagatac gatagaaaca atagggtctg caatcaatga agagatcctg atgcaaattg
    14581 aacatgccca agagactcgt ggagtgatcg tttttaccga tcctgatttt tcaggagaaa
    14641 aaataagaaa aatcattatg gaagctgttc ctcaggcaaa acatgctttt ttgccgcgca
    14701 cacaagctaa accaaagaaa aagggaagcc ttggtgtcga gcatgcaagt gatgctgcta
    14761 ttttagaagc attgaaaaag gtcgtgacgc cagacatttc ccaaacggct gtttctgaaa
    14821 tcactagaca agacctttta gactatggtt taatagctgg ggcacaagca aaagaaaaac
    14881 gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa cggcaaacaa ttggaaaaac
    14941 gtttgaagat gtttcgtatt acgccgaaag aactcgccca agcaatgaaa atagtggagg
    15001 aacaacatga gtgaatatcg tgaaattgcg acaccctcta gaacaaaaga gattttattg
    15061 aaacatggtt tttcatttaa aaaaagttta ggacaaaatt ttctgactga accaaatatt
    15121 ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata cgaacgtgat cgaagttgga
    15181 ccaggaatcg gtgcattgac ggaacaatta gccaagcatg caaaacaagt cgtagcattt
    15241 gagatcgatg atcgtttgat ccctgtattg gctgatacga tgcaacccta tgacaatgtg
    15301 actattgtac atcaagatat tttaaagacc gatttgtcaa cagctgttag agaaacattt
    15361 catgaggagc ttccgttaaa agtcgtagcg aatttacctt actatattac gacaccgatc
    15421 atgatgcatt ttcttgaatc agatttggtt gtggatgaac tagtcgtcat gatgcaaaaa
    15481 gaagtagctg atcgtatctc tgctgaacca ggaacgaaag cttatggttc attatcgatt
    15541 gctgttcaat attatatgga agccagttta gcattcatcg taccaaaaac agtatttgtc
    15601 cctcagccaa atgtagattc agcgattttg aaactgacaa gaagagatat accggcagtc
    15661 gaagtgacag acgaaaaagc atttttccga ttgacaaaag cggcatttca gcaaagaaga
    15721 aaaacgcttt ggaataactt gcagcattcg tatggcaaag atgaccagac aaaagcatgg
    15781 ttagcaaaaa gcttggaaac agctggaatc gatccaaaac gtcgtgggga gactttgtca
    15841 ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa atatgcagta aaaaaagagg
    15901 ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa
    15961 atagttcctc atgtttttcg tttgtttggg cttgttaccg cagatgcaag cttttgaaca
    16021 ccgtttaaga aagaggttgt gggcctgccg tttaactcta agtcacaagg aacaatttca
    16081 aaaaacagct tctcatgttt ttgagttagt tggtcttatt acttgatgca agcttttgac
    16141 cactattatg ccgaacaagg agatgtgaca caaattttgt cacatctcct atttttgttt
    16201 cttgatcaaa aaattcttat acttgatact cacactcatt ttgtatggat ttagaaaaga
    16261 atatgtgtct tgacagttat tagtaagtga gttatactaa gtcgacattt atgtgtcaag
    16321 acatattagg agagagaaaa aatgagaaaa aattcagttc gaaaactaac gatttctgct
    16381 ttattgattg ggatgggggt cattatccca atggtcatgc ctaaaatcat gattccacca
    16441 gcttccttta ctttggcgtc tcatgtgcca ttatttattg cgatgttttt tacaccagga
    16501 gttgcagtgg ctgttgcgct aggtacgaca tttggtttct ttttaacaac gccggttatc
    16561 atcgcattgc gagcattgtc acatcttgtg tttgcgttga ttggggcttg gtacttgcaa
    16621 aagcatccga gtatcgtttt aaaaaatgga cagtttactt ttgctaattg gcgtttccaa
    16681 gggttcaatt ttgtgatcgg tgtgattcat tctttagcag aaatgctagt agtcagcatc
    16741 ttttacttta ttggtaatat gcctgaaaca tattattctc aaggatattt ttatacagta
    16801 ttcatcttga tggggattgg tgggcttatc cacagtttag ttgattataa tattgcttat
    16861 tttatagcag gaacactcag caaatatttt gatatccccg tatttacggc agcaaaaaaa
    16921 gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa cgacatatta gacatgatct
    16981 ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc ctctaagatg agagtttttt
    17041 gtcctaaaaa gagctgcacc aacatcttct ggcacagctc taacaggggg caatttatgg
    17101 aagtttgatg acttgttctg tttctttatc aaagaagtgg cctttgctga tgttgaaggc
    17161 aaggtcgact atttcgcctg gtttatggaa atctcttgca tctacttttg agatgaactc
    17221 agtagaacca gtttttgtgt aaagcatcgt ttcagctcca agtaattcgg aaacgacaac
    17281 ttctgatttc actacggctt caggtgaagc ttcaagggct acttgttcac tgtggatgtc
    17341 ttctggacga ataccgaaga taacttcttt tccttcataa cctttttcaa ctaatacttt
    17401 gttttttcct tcaggaatac gaagcttaag cccatgtcca tctgtgataa caccattttt
    17461 caatgtcaca ttaaagaagt tcatggcagg agatccaata aagccggcaa cgaacatatt
    17521 gacgggtgta tcataaactt ctttcggtgt accgatttgt tggataaatc cgtctttcat
    17581 gataacgatt cggtcagcca tagtcattgc ttctgtctga tcgtgagtaa catagatggt
    17641 cgttgtttca agacgctggt gaagtttggc aatctcagca cgcatggcta cacgtaattt
    17701 agcatctaaa tttgataatg gctcatccat taagaagact tttgcatccc gaacgattgc
    17761 ccgtcccaat gccacacgtt ggcgttgtcc accagataaa gcagctggtt ttcgtttcaa
    17821 gtattctgtc agacctaaga tctctgctgc attgtccaca cgttttttga tgtctgcttt
    17881 atcatattta cgtaatttca agccaaaagc catattatca aaaacagtca tatgaggata
    17941 caatgcgtag ttttggaaaa ccatggcaat atcacggtct ttaggtgcca cgtcattcat
    18001 gacttttccg ccgatattta gttctccttc agagatatct tccagtcccg cgatcattcg
    18061 caaagtagtt gactttccac aaccggaagg accaacaaaa acgataaatt cacggtcttt
    18121 gattgataga ttaaaatcag ttacagaata attttctgca ttgtcatatt ttttatatac
    18181 gtttttcaga gccatttcta ccataatacg ttccacactt tcttttttag ttttattaac
    18241 tatttaaagt ataaatgaaa gcgttaacta acttcaatgt catgttgaac aagaattttt
    18301 tctttttttt gtcaatgtgc tagttttaag ctttaataaa tatccataag aaataaaaac
    18361 tgatacactg gagtaaataa gtatgttaac aatatcaaaa ggagtgtctc ttatgaaaat
    18421 cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg aaattagcaa cagcgtataa
    18481 aacaatattg gaaaaacatg aactttttgc taccgggacg actgggcaga ggatcacgga
    18541 agcaaccggc ttgccgatcc atcggtttaa atccggaccg ttaggcgggg atcagcaaat
    18601 tggagccatg atttcggagg atcagttaga tttggtgatt tttcttcgag acccgctggc
    18661 tgcacagccc catgaaccag atgtaacagc acttatccgt ttatctgatg tctatgagat
    18721 tccgttagct acgaatatcg gaacagctga ggtgctacta agaggactgg aagccggctt
    18781 cctagatttt cgaagaatcg tccatgaaat agacaagcat ccactttcat tttgatggat
    18841 aagcgtatgg aaaaataacg tttaatgttc gcctatatgt agctataaaa gctaaacaaa
    18901 ctgataatat gcgatataat acgaacgtat tggataatat aaacaataat attagttttt
    18961 cgtatttgca gtaaatgtga aaatatgcta tagtttctgt gggaaaagat cccaaaaata
    19021 atacagagta gaaaataaag cgttaagtgc taagaggatg ggatgttgcc tcttggacga
    19081 aggacgtaat gttcgcggtt ttattttcgc attcgctgca tgatacgatg tagcagctct
    19141 taaaggagat gctaaaaatg aagaaacacc ggttagacgc acgtatgatt gctattatgg
    19201 gcttattgat tgcattgatg gtcactttgt cccgactggt agcaatcgag accccattta
    19261 ttaaaatcag tgtcacattt attccacaag tgatcatggg cattcttttt ggtccttttt
    19321 ggtcagggat aggcgcagtt ttagctgatt tagtaggaat ggccctcttc tcgaaatctg
    19381 cattctttat tggatttaca ttgaatgcat ttattgaagg agcaatctac ggattctttt
    19441 tctatcgaaa agagatcact tggaaaaatg ccattctggc tacgcttagt gtcacgctga
    19501 tcatcaactt atttttgaca ccattatggc ttgcgttgat gtaccatgtg ccattgttca
    19561 gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat atggttaccg attcaatcaa
    19621 ttgccattta ttacgtagga cgtagtattc cttacaaaaa aattttaaga agtttagcga
    19681 tccacgctaa ataaaaaaca agcagtcttc ccatcccaga agactgcttg tttttattgt
    19741 gctgtgaatt gtgcttgata gagccgtcgg taataaccat ctttttgaag cagctcatca
    19801 tgggtgccca tttcgacaat agctccctga tccatcacta atatctggtc cgcatttttg
    19861 attgtggaca ggcgatgggc aatcacaaaa ctggtttttc cttccatcat tgctaagaag
    19921 gcttgttgga tttttttctc tgtcaatgta tcgactgagc tggtagcttc atctaagatc
    19981 aacatagggg gattactgat catcgttcga gcaatcgtca acagttggcg ttgtccatct
    20041 gatatcttta acccttgacc gccaatctta gtatctaatt tttccggcaa acgttctaca
    20101 aattcataca tataagaggc ttttagggca tcattgattt ctgcgtcact tgcttccgga
    20161 ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata gccaagtatc ttgtaacacc
    20221 attccaaaac ttttacgtaa tgtatcacgt tggatagagc ggatgtcatg gtcatcgact
    20281 gtaattgttc cttttgttgt gtcataaaaa cgcatcagca aattaacaag tgtggatttt
    20341 ccggcccctg tttttcctac tatagcaatg gtttgacctg gataagcagt aaaattgaaa
    20401 tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa catgatcaaa agagactttt
    20461 cctttgatat ttttgggttc caaggcatag gcatctggca aatcaggctt ttcttctggc
    20521 tgatcgatca aatcaaatgc tcgttctaga ccagaaaaag cggtttgcag ttgtgtcgtg
    20581 attccagaaa gttcgataaa tggtttagaa aactgacttg agtagatcgt aaagctggaa
    20641 atcacaccaa cagtgattga cgaaccactc ttcaatgcca ataggccgcc aactaaacca
    20701 atcgataaat aagcaaggtg atctacaaaa cgagaagacg ggtttgtcaa ggaagaagaa
    20761 aattgtgccc gttgtccttt ttcatataga tcagcattta gcagttcaaa ttgctgttga
    20821 ctagcttctt cttgctgaaa agctttcacg attttctggt taccgattcg ttcggtcaca
    20881 aagcccgaga tatccccaat aatcctttgc tgtctatcga aatttgtttg tgaagcacga
    20941 gcaacgatcc aactcacaag gaaaataatt ggtgtagaac aaagaacgac caatgtcaga
    21001 agtggactta gacgcatcat aaaaataaac gaaagaatga tgattgccac tccagaaaat
    21061 aattgattga aggccgcaga aattgcaatg gaaatattgt ccatatcatt tgtaaatcga
    21121 ctgacgatat ttccgtggga gttttgatca taataacgaa gtggcaatcg attcagatga
    21181 gcaaatgcat cttttcttaa ttttgcaacg gataagtaag ctaaccgatt gcttaacact
    21241 tggatgagcc attgactgac tacgttgatt aaaacgatac caccgaatag aataagtaaa
    21301 tggaacagtc gattgaagcg gacctgtcct tctccaatca tttgatcgat actttgacca
    21361 atttgtaacg tcatcacaac tgttgcaatc ccattgatga ttcctagtat cacagctagc
    21421 caaatgtctt ttgcataggc agtaaggtaa ggaatgaatc ggcggagcgt ctgcaaggat
    21481 aattttttta ctttcataac tattcttcct cctgtgatgc gacgatttcc tgatattctg
    21541 ctgattgagc aagtaattct tcatgggtac cctgagcagc aatcttacct tgatccatca
    21601 ctaagatatg atgtgcttct tgaatagagc ggacacgttg ggagataata atcactgtac
    21661 tgccaagata agtctgtagc gccttacgca aatccaagtc tgtttgataa tccaacgcac
    21721 ttaaagagtc atctaaaatc agtaatgctg gcttggcaat caaagctctt gcaatcgtca
    21781 gtcgctgttt ttgtccgcca gagaaatttt tcccattttc catgactttt gtatctaaac
    21841 catttggaag ctgacggaca aattcttctg cctgagcgat tttcagagct tcccaacatt
    21901 ctttttctgt ggcgtctttc ttaccccatt gaaggttttc acgaatagtc ccggtaaata
    21961 gtacagaagt ttggggaaca agagcaattt gctgacggag atgatccagc tgccaagaac
    22021 gaacatccat tccgtccatt ataactgtac cggaactgac atcatagaaa cgcggaatca
    22081 attgtgtcaa agtcgttttc ccgcttccgg ttggaccaat gattccaagg actgtatttc
    22141 gcttcagctg aaatgtaata ttttggagag aaagaccaga atcaggagta taacggaagt
    22201 caacatggtc aaaagcgata attgcttcag aagaaaaatc gggagtagta ctgacctcag
    22261 cttcaagaat cgaattttct gtgtctagca cctctttcac ccgatttcct gaagcttcgg
    22321 cacgtgtgaa gatcacgacg aggtttgaaa caacgatcag tgccaaaagc atttgattca
    22381 tataattgat caaagcaaga acttgtcctt gttgcaagct accaatattt actttgatcc
    22441 cgccaactgt cagcaaacag atgatcccga cattcatgat caaggttgta gcaggtgata
    22501 atagggcaga gatattggaa acacgtaaat aattgttcgc tagatcattt gtgcttttat
    22561 cgaacttctc tgtttctttt tttgtctgcg caaatgcgcg gatgacacga acaccgctaa
    22621 gattctgact aacgtgttca ttgagctgat ctagtttttc ttgcactttt tggtagagag
    22681 gaacggtttt tttgatgata aaaaataaaa tgattgagaa aataggcaat aatgctaaga
    22741 aaaacagacc tacttcccag tcgatcacaa aagccatgac cactgacccg atacttaaaa
    22801 acggtgctct gatcaataaa cgaatgacca ttgctaaagc tagctgaagc tgattgatgt
    22861 cgtttgtcat tcgggtgatc aaggtatctg ttccaaaatg attcaactcg ttgtgagaga
    22921 aagtattgat ttttttgatt agctgattac gtaactctgt accaaatcct tgtgaagcta
    22981 ctgaagcata gtattggcaa atgatagcac aaatcagacc aatcacagac atagcgacca
    23041 tccataagcc catttcaatg atttttcctc ggtcattcat ttttagtcca ttatcgatca
    23101 aggaagccat cattaaaggc aggacaagtt cgaagacagc ttcaagaaat ttgaagaacg
    23161 ggcccaaaat gatttgctta cgatagtctt ttgcataacg taataactga atcatatgat
    23221 tcctcctatt atttatttgt ccacaattga aaaaacaagt actaccacat tgtagcttag
    23281 gaaatttaga atgaaaagag aacaagcctt ttcataaatg aaagttttat tatgaagaag
    23341 ctttagaaat tgaatagact atttttttta aaaaaaagaa aat
//
LOCUS       DAPCMT010000030        23067 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPCMT010000030 DAPCMT010000000
VERSION     DAPCMT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.030"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(111..566)
                     /locus_tag="RD743_001001"
     CDS             complement(111..566)
                     /locus_tag="RD743_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562829.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="RD743_001002"
     CDS             complement(720..1928)
                     /locus_tag="RD743_001002"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDU0562830.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="RD743_001003"
     CDS             complement(2040..2372)
                     /locus_tag="RD743_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDU0562831.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="RD743_001004"
     CDS             2520..3395
                     /locus_tag="RD743_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDU0562832.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSGELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="RD743_001005"
     CDS             complement(3477..4058)
                     /locus_tag="RD743_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDU0562833.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="RD743_001006"
     CDS             complement(4064..5323)
                     /locus_tag="RD743_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDU0562834.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="RD743_001007"
     CDS             complement(5325..6371)
                     /locus_tag="RD743_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562835.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="RD743_001008"
     CDS             complement(6606..6881)
                     /locus_tag="RD743_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDU0562836.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="RD743_001009"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="RD743_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDU0562837.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="RD743_001010"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="RD743_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDU0562838.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="RD743_001011"
     CDS             complement(9980..10660)
                     /locus_tag="RD743_001011"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDU0562839.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="RD743_001012"
     CDS             complement(10709..11344)
                     /locus_tag="RD743_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDU0562840.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="RD743_001013"
     CDS             complement(11410..12813)
                     /locus_tag="RD743_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDU0562841.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="RD743_001014"
     CDS             complement(12800..13831)
                     /locus_tag="RD743_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0562842.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="RD743_001015"
     CDS             13862..14083
                     /locus_tag="RD743_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDU0562843.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="RD743_001016"
     CDS             complement(14248..14985)
                     /locus_tag="RD743_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDU0562844.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="RD743_001017"
     CDS             complement(15138..16478)
                     /locus_tag="RD743_001017"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDU0562845.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="RD743_001018"
     CDS             complement(16715..18238)
                     /locus_tag="RD743_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDU0562846.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="RD743_001019"
     CDS             complement(18616..19227)
                     /locus_tag="RD743_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDU0562847.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="RD743_001020"
     CDS             complement(19557..20273)
                     /locus_tag="RD743_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDU0562848.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="RD743_001021"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="RD743_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDU0562849.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="RD743_001022"
     CDS             complement(20832..21629)
                     /locus_tag="RD743_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDU0562850.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="RD743_001023"
     CDS             complement(21599..21970)
                     /locus_tag="RD743_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDU0562851.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="RD743_001024"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="RD743_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDU0562852.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6469 a         4926 c         3911 g         7761 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctggt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacaat tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt ttttcggctc tataatttat gggactgatg aatcagaatt gattcttcag
    23041 ccccattttc attttaggta ttaaaaa
//
LOCUS       DAPCMT010000031        22825 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPCMT010000031 DAPCMT010000000
VERSION     DAPCMT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.031"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            33..257
                     /gene="nrdH"
                     /locus_tag="RD743_001025"
     CDS             33..257
                     /gene="nrdH"
                     /locus_tag="RD743_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDU0562853.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            416..2575
                     /gene="nrdE"
                     /locus_tag="RD743_001026"
     CDS             416..2575
                     /gene="nrdE"
                     /locus_tag="RD743_001026"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDU0562854.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2625..3590
                     /gene="nrdF"
                     /locus_tag="RD743_001027"
     CDS             2625..3590
                     /gene="nrdF"
                     /locus_tag="RD743_001027"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDU0562855.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3679..4818)
                     /locus_tag="RD743_001028"
     CDS             complement(3679..4818)
                     /locus_tag="RD743_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDU0562856.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5127..5840)
                     /locus_tag="RD743_001029"
     CDS             complement(5127..5840)
                     /locus_tag="RD743_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDU0562857.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6094..6795)
                     /gene="nagB"
                     /locus_tag="RD743_001030"
     CDS             complement(6094..6795)
                     /gene="nagB"
                     /locus_tag="RD743_001030"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDU0562858.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7029..8561
                     /locus_tag="RD743_001031"
     CDS             7029..8561
                     /locus_tag="RD743_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDU0562859.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8752..9954)
                     /locus_tag="RD743_001032"
     CDS             complement(8752..9954)
                     /locus_tag="RD743_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0562860.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10036..10725)
                     /locus_tag="RD743_001033"
     CDS             complement(10036..10725)
                     /locus_tag="RD743_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDU0562861.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10831..11199)
                     /gene="rplL"
                     /locus_tag="RD743_001034"
     CDS             complement(10831..11199)
                     /gene="rplL"
                     /locus_tag="RD743_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDU0562862.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11252..11755)
                     /locus_tag="RD743_001035"
     CDS             complement(11252..11755)
                     /locus_tag="RD743_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDU0562863.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(11959..12086)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(12165..12854)
                     /gene="rplA"
                     /locus_tag="RD743_001036"
     CDS             complement(12165..12854)
                     /gene="rplA"
                     /locus_tag="RD743_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDU0562864.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12958..13380)
                     /gene="rplK"
                     /locus_tag="RD743_001037"
     CDS             complement(12958..13380)
                     /gene="rplK"
                     /locus_tag="RD743_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDU0562865.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13723..14391
                     /gene="sdaAB"
                     /locus_tag="RD743_001038"
     CDS             13723..14391
                     /gene="sdaAB"
                     /locus_tag="RD743_001038"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDU0562866.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14404..15276
                     /gene="sdaAA"
                     /locus_tag="RD743_001039"
     CDS             14404..15276
                     /gene="sdaAA"
                     /locus_tag="RD743_001039"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDU0562867.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15367..16386)
                     /locus_tag="RD743_001040"
     CDS             complement(15367..16386)
                     /locus_tag="RD743_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDU0562868.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16559..17464)
                     /locus_tag="RD743_001041"
     CDS             complement(16559..17464)
                     /locus_tag="RD743_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDU0562869.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(17523..18068)
                     /gene="nusG"
                     /locus_tag="RD743_001042"
     CDS             complement(17523..18068)
                     /gene="nusG"
                     /locus_tag="RD743_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDU0562870.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18169..18339)
                     /gene="secE"
                     /locus_tag="RD743_001043"
     CDS             complement(18169..18339)
                     /gene="secE"
                     /locus_tag="RD743_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDU0562871.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18363..18515)
                     /gene="rpmG"
                     /locus_tag="RD743_001044"
     CDS             complement(18363..18515)
                     /gene="rpmG"
                     /locus_tag="RD743_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDU0562872.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18677..19321
                     /locus_tag="RD743_001045"
     CDS             18677..19321
                     /locus_tag="RD743_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDU0562873.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19377..19880)
                     /locus_tag="RD743_001046"
     CDS             complement(19377..19880)
                     /locus_tag="RD743_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDU0562874.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19893..19939)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20477..21442
                     /locus_tag="RD743_001047"
     CDS             20477..21442
                     /locus_tag="RD743_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDU0562875.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21541..22713)
                     /locus_tag="RD743_001048"
     CDS             complement(21541..22713)
                     /locus_tag="RD743_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562876.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22700..>22825)
                     /locus_tag="RD743_001049"
     CDS             complement(22700..>22825)
                     /locus_tag="RD743_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562877.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         7135 a         4584 c         4105 g         7001 t
ORIGIN      
        1 ataataagaa tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata
       61 attgtatgca atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag
      121 aagtcaatat cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa
      181 gtgttcctgt cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc
      241 gtaaacttgc cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc
      301 ttgagacagc accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa
      361 tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag
      421 tcttaaaaat ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt
      481 caatggtcaa atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa
      541 tgtcaaacca aatacgatga cttttctatc tattatggaa aaaatcaatt acttgatcga
      601 tcaagaatat atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct
      661 atatcaattt ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt
      721 ttattctcaa tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga
      781 tcgagtagcc ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct
      841 tgcagacgaa atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg
      901 acgtaaacgt cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa
      961 ctctatcggg cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg
     1021 gattacttta tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc
     1081 aagtggtgtc gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt
     1141 aggtcagcgt caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc
     1201 tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct
     1261 tggcgtgatc gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt
     1321 atttagtcca tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac
     1381 agctgagtat gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg
     1441 tgatcttgaa aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa
     1501 tattgataca gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg
     1561 ttcagagatt ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt
     1621 attaggaacg gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag
     1681 ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc
     1741 atctgatatc gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg
     1801 attaggtgcc atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga
     1861 ggattcatta gactttacaa atatttactt catgctgttg aattattgga cacttgttga
     1921 aagcaacaat atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta
     1981 tgctgacggc acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa
     2041 agtcaaagca ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa
     2101 acaagcagta atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg
     2161 ctcaatttca tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga
     2221 agaacgccaa gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga
     2281 gacgattccg tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta
     2341 tgcaatggca caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga
     2401 aatcccagaa ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa
     2461 tattttaaga cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt
     2521 tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca
     2581 tagatagatt cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt
     2641 atgaagcaat aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc
     2701 tgacagagca attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc
     2761 gtacattgtc tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc
     2821 tagatactgt ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc
     2881 atgaagaagc cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt
     2941 cttctatttt tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa
     3001 atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta
     3061 caccgctaga aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt
     3121 tttatactcc tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca
     3181 aactaatcat ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag
     3241 gattcaacga gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc
     3301 tttatgagct ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct
     3361 ggacagaaga agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag
     3421 ggatggatcc actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga
     3481 tttcaacagg cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag
     3541 gtcatgtaga agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt
     3601 taattcaaaa atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca
     3661 ctgaactctt ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt
     3721 ttccgaaaca tgttccattc ttctcaataa atagattttc cggatattta atattatcgt
     3781 cttaatcact gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc
     3841 tgctccaatc aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa
     3901 caacggcttg atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata
     3961 caaagcttga aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa
     4021 agggccgaca tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata
     4081 aggctgatcg aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa
     4141 gatcgtactg ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa
     4201 ctctttacga atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc
     4261 cttgaacata aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat
     4321 aaatgaaatg atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt
     4381 actaagagat aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc
     4441 ccgctgcgcc aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg
     4501 ggtcaattga attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac
     4561 taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt
     4621 ttcgagaaaa acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg
     4681 taaaaaaact gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat
     4741 aaagtacacg ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg
     4801 tttaaaacgt gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt
     4861 aacaaagagt tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata
     4921 ttttaatgag tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat
     4981 actgcagtcc ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc
     5041 agccaaacga aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg
     5101 acatctattt tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg
     5161 atatttttgt gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga
     5221 tcaatcgtat agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc
     5281 acatcttcga ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct
     5341 tttctatgga taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc
     5401 actactgtga gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc
     5461 atgccgatgg cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc
     5521 gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc
     5581 acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct
     5641 aaagcatctc ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct
     5701 cccatacaaa ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca
     5761 aaaccgattg ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt
     5821 gtcaatacca tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag
     5881 cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt
     5941 catagtcctt tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa
     6001 acggcaaaat atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct
     6061 gatttcacaa cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc
     6121 gatgatcaca acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac
     6181 aggtccttca atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac
     6241 gatttcttta cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc
     6301 ttctgcttta tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac
     6361 tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc
     6421 gattcctaag atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca
     6481 tgctgcttcc agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt
     6541 atcaaataat tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc
     6601 aacgtactca tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat
     6661 ttctttatat aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt
     6721 gttcatacct tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac
     6781 acgaatgatt tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc
     6841 tttttttctt tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca
     6901 ataaatagga atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat
     6961 gaaaaccaag caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag
     7021 gagttattat gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc
     7081 tctatgaagg tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa
     7141 acggagccgg taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg
     7201 gacagatcca ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg
     7261 atgaaacatt aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg
     7321 aagaaaaaat tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg
     7381 aaaaagctgg gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct
     7441 tagttgctga gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga
     7501 aaaatctaag tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc
     7561 cagatgttct gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc
     7621 tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat
     7681 ttcttgatca gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata
     7741 ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc
     7801 aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag
     7861 ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc
     7921 tgacgcctcc agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca
     7981 cgacattagc agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac
     8041 ccatcaattt gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg
     8101 gaaaatctac tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc
     8161 acttcccaga taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat
     8221 tagaaacacc actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac
     8281 gtcgccaact agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat
     8341 taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt
     8401 tactgattct cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag
     8461 acgggataca gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc
     8521 aagatcttgt gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg
     8581 aaaaaaagga gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc
     8641 aattagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt
     8701 tggctattct tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt
     8761 attttcgaga tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg
     8821 aaaaacaagc ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc
     8881 tagcaatgaa ccagccgcac ctccaataaa gtacataaac ataaatacag tattatttcg
     8941 gttgcttgct gcttcaccta gcccttgtac tcgtgtttga ttggcgactt gaccaaattg
     9001 attcccgacg tctaataaaa taatagctaa aagcaaaatg gccaaatggc ttccgccaac
     9061 caataaagca ataaagctta ttgtttgcat aacaagtccc atatacacga tttttctttc
     9121 agaataagaa tctgccagtc gtcccacgat aggtgcagca actgctccag acaaaccaaa
     9181 aatcgctaat atccctgctt cgaatgttcc ccaattgtag gcgggactgc tgatataaaa
     9241 aatcaacgta gaccaaaaga tcgaaaatgt tccaaacatg aaaaatccac tgactgccgc
     9301 ttctcttaat agacgttgag aagcaactaa atgcggcaaa cttttaagtg aatcaaggta
     9361 tgaaacagct gtactacttt gctggacaga acgcggcatt ttccagtaaa gcagaagtgt
     9421 aagaattccg actgcacata aagcaaataa atagatcatc cgccaagaaa aaagactggc
     9481 aacaatacca gataccgtcc gagaaagcaa aatccccacg agtaaaccgc taagcaatgt
     9541 acccatgacc ttccctcttg cttttggtcc cgctagtacc gcaccgtaag gaatgatgat
     9601 ttgcggcacg atcgaaagta atcctattaa aaaagaagcg catgcaaata acgtaaacga
     9661 aggcgcaaaa aaagcggcca acagagagac ggcagataac gctgccattc gaatgatcaa
     9721 ttttggacga tccaccacgt cccctaaagg aactaaaaac agtaacccta acgcatagcc
     9781 taattgggtc aacatcgtca atatcccgac tgcacttgtg ctaacagata aactagctgc
     9841 tacttttgtt ccaatcggct ggatgtaata catattggca acaacaacgc cacaagtgat
     9901 tgataatagt aaaataagcg aagttgttaa aggttttttt tcattactct tcattgattt
     9961 cccccatttc ctatcttagt ttacaactta tggaaaagat tggaaataga aaaatacaac
    10021 tattcgccac acttattatt tgtgcttttg ttttccttgt tttttcttgc gatctttata
    10081 aatgaagaca actaacccta ctagcacagt aacacttgca accaacgtga ctgcagtttt
    10141 gttatatccg tgaaacagag cttctccacc acacgcatac aacacagacg aagggatgac
    10201 tccaatcacg atcgacaata tgatttgctg tagaggcaat ttcatcgcat ctgcagcaaa
    10261 attgaccata gaagatggaa taaccggtat catatagcct aacattacac ctatttttgg
    10321 atgtttcatt tgtcggattg cttgcaccca atgattcgtt tcggttttct tatctaaaaa
    10381 ttttaagcga tgcataagga aaattgcagt gatgttacca agagcattac ccaaaatgtt
    10441 gatgatcgca cctgtcagtg gtccaaagct caaaccagca atgactccaa ctactgaagt
    10501 aggaatacct ggaacagcac acatcaccgc tgtcaatacg atcaagagta tcgatgcaaa
    10561 agtcccatgc gaacgaatat gtgcgagtag ctctgcctta ctcgctctcg gatttaaaaa
    10621 taattggatg tctgtctgat attctaaata caaccgatag cccactacca gtatcagaat
    10681 gattcccaac gagatcaata tcaatcggat tattttcttt ttcatatttc ctcctgaaaa
    10741 aaatctatat tcattaaata atagtttaac ataaaaacaa aaaagcaccg aaatccaaca
    10801 taggatctca gtgcttttaa atagtaaaaa ttatttaaca gttacagatg cgccaacttc
    10861 ttcaagttta gctttcaatt cttcagcttc ttctttagat acgccttctt taactggtgc
    10921 aggagcgcca tcaactacag ctttagcttc tttcaagcct aaaccagttg cttcacgaac
    10981 tgctttgata actttaactt tttggtctcc agctgcagtt aattctacag tgaattctgt
    11041 ttgttcttca gcagcagcag cgccaccagc agcagctaca gctacaggag cagcagcaga
    11101 tacgccaaat tcttcttcaa tagctttaac taggtcgtta agttctagga tagttgcttc
    11161 ttttaactca gcaacgatgt tttcaatgtt caatgccatt ataatttcct ccgtttaatt
    11221 gttctttttt atttaatgct ttatgcggca attaagctgc atctccatct tctttgtctg
    11281 ccactgcttt gacagcgtaa gccacgttgc ggactggcgc ttgtagtaca gatagcaaca
    11341 tagatagaag tccttcgcga tttggcaatt ttgccaatgc tgtgatttct tccaatgaag
    11401 agactttacc ttcgatgata ccgcctttga tttctaatgc ttttgcttct ttagcaaatt
    11461 cgtccatgat tttcgctggt gcgactacat cttcgttact gaatgcaaca gctgtaggtc
    11521 cagtgaatac ttcgtccatg ccttcaaggc cagctttttt agctgcacgt gaaagaatag
    11581 aatttttgat aactttcatt tctacgttag catcacgaag ttgtttacgt aaacgtgtta
    11641 cttcatcaac tgttaatccg cggtagtcaa cgactactac tgatgcagct gctgtaaatt
    11701 tttcgcttac ttcatcaacg attgctgctt tttttgcgat aattgcttca ctcatttttg
    11761 agtttcacct cctgaatttt gatggaagag ttttttgcac tgaaaaggca agtcactttt
    11821 atagttactt tgctcaacca gtattattca tcacctattc acttcgttca tagtgattct
    11881 actcgctttg ctcgaaagga aactcctttc gctttgctca accagtattg ttcatcatct
    11941 cttcacttcg ttcatagtga ttcacaataa aaaactctat gccaccgcag acatagaggg
    12001 actattgaat atattcaata aacgtcctcg gtaggaaatt aaggcttgat gccacctact
    12061 gtcttcggta caagttaatt attaacctca actagcttag catgctaaac tagttgagtc
    12121 aagaattatt tttcagttgg atcaatcaac attctaatat ttaattagaa agaagcttgg
    12181 tcaacatgga taccaggtcc aaatgttgtt gtgatcgtta catttttcat gtattgacct
    12241 ttagctgctg atggtttagc ttttagcaat acatcatgga ttgctgcaaa gttttcaact
    12301 aatttttcat cgtcaaatga aacttttccg attggtacat ggatatttcc agctttgtcc
    12361 acacggtaag ttactttacc agctttcact tcgtttactg cttttgttac gtccattgta
    12421 actgtaccag ttttagggtt tggcattaag ccttttggtc ctaatactcg tcctagacga
    12481 cctacagtag ccatcatatc aggtgttgca acaactacat caaagtcaaa ccatccgttt
    12541 tggatttttt gaaccatgtc gtcgtcacct acgaaatctg caccagctgc ttcagcttct
    12601 tttgctttgt ctcctttagc aaaaactaaa acagtttgtg ttttaccagt tccgtttggt
    12661 aatacaacag caccacggat ttgttggtct gcttttttag ggtctacgtt caaacggtat
    12721 gcaacttcaa ctgttgcatc aaattttgca atgtttgttt cttttgctaa agctactgct
    12781 tcggcaacag gataaacttt acttgaatca acttttttta atgcatcttg catttttttg
    12841 ctctttttag ccattttggt tcctccttga ttgtggttat aacggttgta cctcccacgt
    12901 gttctatatc tttcaatata gagccaactg agaagctgct gtcttagggt gtatagatta
    12961 ttctacagtg atccccatgc ttcgtgcagt tccttcaacc atgcgcattg ctgcttcaac
    13021 gttagctgcg tttaggtctt ccatttttaa ttcagcgatt tctcttactt gatcgctaga
    13081 aactttcgca actttatttt tgtttggttc gcctgaacct ttttcgattt tagctgcttt
    13141 tttcaataga acagcagctg gcggtgtttt tgtaacgaat gtgaatgaac ggtcttcgta
    13201 tacagaaata acaaccggaa tgatcaaacc tgcttgatca gctgtacgag cgttgaattc
    13261 ttttgtgaat cccatgatat tgatacccgc ttgacctagt gcaggaccta ctggtggagc
    13321 tggagttgct ttacctgcag gaatttgcaa tttaacgatt ttttctactt tctttgccac
    13381 gagacatacc tccttaagtc cgtgatgtgg ttaattggag atgcatattt ctcctcccac
    13441 gttttatctg atctgaaaaa atccagacaa ataatgtgca aaacaatgca cactggtaca
    13501 gtatagcaaa tttatttgtt ttttcaagat ttttttctgc tatttttaca ttttgcttga
    13561 ataaacgcta aatttattct aaaaaaacaa caatttttcg ttctcatttc ttcttttatg
    13621 ttacacttgc gctagcatgg ttcaacagat gaaagcaaac aataacacgg ttttttagac
    13681 ccttgtacaa agattcctac ataacaaaat gaggtggaga aaatgaaaga tttaaggtat
    13741 agaagtgtct ttgatattat tggacctgtg atgattggtc caagtagctc tcacactgcc
    13801 ggagctgcta gaatcgggaa aatcatacgt agtatatttg gcgaacagcc tgattctgta
    13861 gatatttatc tatatgaatc atttgcaaaa acctatcggg ggcacggaac agatattgca
    13921 ttagttggcg gcctgttagg gatggaaccc gatgatgaac gtttagctga ttctttgaaa
    13981 ttggcatatg aagccggtat ggaagtcttg tttgttccta agagtgaaaa agccgaacat
    14041 ccaaactctg taaagatgct tctaaaaaaa ggaacacgaa agctttcagt cactggcatt
    14101 tcgatcggcg gtggcaatat ccagatttct gaattgaatg gattcaaaat ttctttaagc
    14161 atgggtactc ctaccttgat cgttgtccac caagatgttc caggtatgat tgctaaagtg
    14221 actagtatac tttctgagac caatacgaat atcggcacta tgacagtgac acgggaatca
    14281 aaaggtgaaa atgccattat gataattgaa gtagatgatc ctcaagtaga agaaacggta
    14341 aaaagactca aacagctgcc aaatatcgac agcgtaaatt atttcgatta aggagctaaa
    14401 aaaatgtttt tatcgattga agaattagta gatcaagcaa aagcttatcc aagtgtagca
    14461 gaattgatga ttgcagttga aatggaaatg agtggccgtt cccgtagtca catcatcgag
    14521 atcatggaaa aaaacctctc agtaatggaa caatcaatca acgagggaaa agaaggtgtt
    14581 acctccgtca caggaattac cggtggtgat gcaaaaaaat tagatgatta tttaaatgag
    14641 gcagactttt taagtggaga aacaatctta aaagctgtac gcaatgctgt agctgttaat
    14701 gaagtcaatg caaaaatggg gttgatttgt gctacaccga ctgccggaag cgcgggtgtt
    14761 gttcctggtg tgctgatggc tgtccgagat cggcttgatt tgacacatga acagcaattg
    14821 gaattcttat ttaccgctgg cgctttcggg ttagtcatcg caaacaacgc ttctatcagt
    14881 ggtgcagaag ggggatgtca agcagaagtt ggttctgcaa gtgcaatggc agcagcagct
    14941 ttggtttgtg caaaaaatgg gactcctcat caagcagcac aagcagtagc cattacattg
    15001 aaaaatatga tgggactgat ttgcgatccc gttgcaggct tagttgaagt cccgtgtgtc
    15061 aaacgtaatg ctttaggctc ttctcaagct tttatctctg ctgatatggc attagcgggt
    15121 atcgaaagtg tcattccgcc ggatgaagtc gttgctgcaa tgtaccaagt cggacgtcaa
    15181 atgccgtcga ttttcaaaga aacagcagaa ggtggcttag ctgttacgcc aactgctaaa
    15241 cgacttgcgg aagagatttt aaataagagg gcgtgataaa gctccttaat ttcgagtatt
    15301 aagagattag ttatcttcat aacaaaaaac gaagagagga ctaattgccc ttctcttcgt
    15361 tttttattaa tccatcaatt tcgtattttt tacttcatcg atcgtttttg ttcctgcaag
    15421 ttgcatcgtg atcgacaatt ctttgttcag gtgttcaaaa acagatgtta ctccttctgc
    15481 cccacctaga ttcaacccat aaatcactgg tcggccaatc gcaactaagt cagcaccact
    15541 tgccagagct ttgaagacat gctctcctcg acggacacca gagtcgaaaa tcactggtac
    15601 acgtttgttg actacttcgg caatttttgg caatacttca aaggacgcag gacctccatc
    15661 tagttgacgt ccaccatgat tggataccca gataccatct gcccctgcag agatagcgat
    15721 tactgcatct tctggcgatt gtattccttt tacgataact ggaagatggg tgatttcttt
    15781 gattgttttg atgtcatctg gtgtcaagcc ttgttttgca gctgcataaa tttctgcgat
    15841 tccttttcct tcgccattac cacttgcact ttgttcgcta tacgcagcta agttaggcat
    15901 tggtaatgga aattggaatt gattgatgac gtcttcttca cggtaaccac cgagcgttga
    15961 atctgccgtt aaaatgatag ctttagcccc tgcttctact gctttgttca aaatgaactc
    16021 attgaatctg tcgtctttac tcatatataa ttggaaaaac tgcggtgcgc ctggcgctgc
    16081 atcggctgcg tctttgattg tcgtatttgc ataagtgctg atggaaaaga ttgatccagc
    16141 agcagctact cctttagctg tatcagcttc ccctttttca tgtgccaatc cttgggccgc
    16201 tgatggcgct tggataatcg gtgtatctaa ttcaattccg aaaacagatg tatgcaaatc
    16261 ggctgagtcg attccgcgta atatccgtgg catgattttc tttgtattaa aagaagtcgt
    16321 attttctttc atcgtccact catcttcaga accaccgcga atatagccaa aagctcctgc
    16381 ttccatgcgc tcttttacac gaccttctag ttaggcaatg tttacaatat cgattggatg
    16441 ttcttcatta cttgtatgat aagtcatagt agatccttct ttcatcttct tgattacata
    16501 taaatcatac taaacttaca aaaagtaagc aaacatttac tctcagatac ttttatcgct
    16561 attctttcca aataaatcga cttacaagct gatctacttc ttggttttca tgaagcatac
    16621 tatgagaggc attttcacct gtcactactt tttcctctat ttcatttccg cgttgcttca
    16681 acaacgcata gaccgctaat gcactgttta acggtactgt tccatcactc aagtctgttt
    16741 catctagttg accagcaatc aagaaaaatc gagtggttat aggaatatta ccgatcattt
    16801 gctggtaatc ttgatagcgg ttagaaacga cagcaggccc ctttgctaac tcatctgtca
    16861 gagactgcgc actatcatcg acaaaatcat taaaaggcgc gccaattgct gttaatgtct
    16921 gaacttgtgg caatgacgta tcctgtccat acgttcctaa atagcgtagt gcactaactc
    16981 ctcccatgga atgccccact atgttgactt tatctatgcc gtagttttct ttcagataca
    17041 aaagacaagt tttgatccat tctgcttgat tccactcgtt atttttatta tcttcaaaca
    17101 atacttggac catcggattg tctgcttttc cagacaaatc tcctgttgct tgtatttgtc
    17161 catctgttcc tactgttaaa accaattctt ttactgcttg ctgatttttt tccaaacgcc
    17221 gcaacatccc tttaaaggaa ttttctgtcc cgctgtagcc gtggaaaaaa agtgttggaa
    17281 tagtggtttt ttctatcact ttactggatt ctgccgtttt ttctttatca gaaacttcag
    17341 tagtatttat cttcgtcgtc tggcaaccac ttaacgcaaa caaactgata aggactaaag
    17401 ctgctgcagt tgcacgtttg cataaatttt tgattggttc tgtcatacac ctcttccttt
    17461 ccatatagaa aaaagaaaga cttgaaaggc caatcatatg atcgacattt caagtctctt
    17521 tattaatcga ttttatctac ttgatcgaaa tcaagttctg tactggtttc acgtccaaac
    17581 atatcgatgt tgactttcaa tttttctttt tctccgtcaa cttctgttac ttgtccttct
    17641 aatccagcaa atgcgccttc gatgattttc acgacttcgc caacttctac ttctagatca
    17701 ttttgtcgca ggctcatacc gatagaacgc aagatatgat tgacttcttc tggaagtaat
    17761 ggtgctggtt tgcttccggc tccgtgtgaa ccaacgaatc ctgtcacacc aggtgtgttg
    17821 cggacaacat accatgaagc gtctgtcatg atcatttcaa caagtacgta tcctgggaat
    17881 gttttatgaa cgatttcttt ttctttccca tttttgactt cagtttcttt ttcttcagga
    17941 acaacgacac ggaaaataaa gtcggccatt ttcatacttt gcgcacgaga ttcgatattt
    18001 gcttttactt tgttttcata tcctgaatat gtatgcaaga cataccagtt tttttcaaaa
    18061 gattccattg aaggctcctc atttcattgt aaaataaaaa aacctcagta cacccgaagt
    18121 ttttctttcg cttcattata gcactaaaag tacgttgtgg ctagcgagtt attttaaaat
    18181 ccagccaaat aagctttgga tggctgtatc cattacgtaa aacatcaatc cgaaaataat
    18241 agaagtttcg atgactacta acgtatcttt acggagttgt tttctgcttg gccaagttac
    18301 ttgtttgatt tcatctttta cgctttttag aaatttcatg agacaacctc ccttgctact
    18361 gtttatttcg tttctttatg taatgtatat tgattacaat atttacaaaa cttattgatt
    18421 tccaaacgtt cgccgctttt tccttcactg actgattttg tgtagttgcg agacccacaa
    18481 acggaacaag cgagtgctgc ttttttcttt gccatttact tctgccccct tgctgagaat
    18541 cagacttata cttcataaca gtatcacgta actttttact tgtcaatttg tgaaataaac
    18601 tatctattgt tttggataaa attatgatat gattaatttg aaattaagct aaaatgagac
    18661 aattggaggt atagcgatgc tattaaaaac acttgtatat aagaaaaaag atctgacaac
    18721 cgttacagag actagtacat tagaagaagc attaaaaatt ttagaaactt ccggataccg
    18781 ttgtgtgccg atcttagatt cttcaggtaa tattttccgt ggaaacattt acaaaatgca
    18841 tatctatcgt cataaagcaa atggcggtga tatgacgctt cctgtcactc atcttttgaa
    18901 aaacgctaca aagttcattt acctggatac ttctttcttc aaagtattct ttacgatcaa
    18961 agagcttcct tatattgctg tccttgacag tgaaaatcat ttttatggta ttttgacaca
    19021 tagtacgttg cttaatatgc tctctcaatc ttggagcgta gaacaaggaa gctatgtact
    19081 gacaattgct tccacaggaa agcaaggaga tttggctgct attgcaaaaa tcatttcaaa
    19141 acacagcagc attgccagct gtattactct tgatatcgaa aaagacgaat acattcgacg
    19201 tacattgatc acgctaccgg ctaacactga agcgaaagta tgtgaaacga tcgtcaacca
    19261 cttagagaaa aagaattata aagtggtaga aattgaaaat ttgcaaaacc aagaagcata
    19321 atcaaaaagc gaaaaaagaa aacagctaat ctgctttctt ttttcgcttt ttataattag
    19381 acacgctttt caaaatcaag cgcgcgatct aaataataca tcaatggtgc tgaaacagcc
    19441 atgacgataa actcacttaa tgcaagcgtg ccataggttg tccaaaatgc ttggccacct
    19501 gttggaacta acatataagc aatcagtccc atgctgatgg taaagaagat cgtgttcaat
    19561 actaaacgca tctttacatt tttcactttg ttttgcaata aagctgtcag gcctaaagaa
    19621 agcaatgttt ggccgccacc aaaaaccaca tccatgatac caaaaccaaa gaaggcatta
    19681 tagacgatga ccccgcctaa aactccccaa agcaatttac ggttaaagac aactaaatga
    19741 ttcaaacttt ctgaaatacg aaactggatc ggccctgagg atacaggcgc aactaaaata
    19801 gttaacgcta gataaagagc catgatgatg ccatttacaa cgattacgga taaccgttgt
    19861 ctgcttttcg ttttttgcat tttctctcct cttttctcct agttttttta acgcgggatg
    19921 gttgatgaac cgcggttatg tgaaacaaac aataattata acagactttt catttatttc
    19981 aatccgtctt atctgctttt aaaaaagcaa aagcttaaac aatgtaaacg tttgctaaaa
    20041 atcgtgggat atggtataaa gtaagtgtaa ggggtcataa gaattcccag taaagcagac
    20101 gaagtaggat ctgcaagaac agaattgttg tgaaacttaa ataaataaaa aaggatgtgg
    20161 gaatttgagc aacctcttac tagagaacgg tctagcaagc agctgaagat ctaagtggac
    20221 atgccgaacc ttggaacttc tccttacgta agtaatggat catgcgcttg ctaggctttt
    20281 ttgtgttttt cggtcttcgg acctttttca ttttcctaga cttctgctat aataaccaag
    20341 aattaatttt gtaacagatt tttttgaaaa aacaagtttc tttcattcaa aaaatagaaa
    20401 aagcttgcta attattggtt aaccagcaaa caggagtatt aaaatatctt gttttattga
    20461 atagcaggag gaaatcatga aattccatta tcatatattg atcaatccat cggcaggcag
    20521 cggaaatggc tataaagtag cagaaagaat tttaccggta ttaaaaaata agcatattga
    20581 ctatacactt cattatagtg aatacaaagg acacgatgct gagattgctg aaactttggc
    20641 aaaagaaaca ttgattcctt ggatagaaga agaacagaaa acaatcgata ctttccccct
    20701 tttgatcatc gtaggaggtg acggcacctt acatcaggta cttgatactt tctatcagat
    20761 ggaagtagaa ttcccagttg cttatattcc agcaggttct ggagatgatt ttgctcgagg
    20821 ggcaggcctt ccaaaaaatc ccaaaaaagg acttcagctg attttagcag ctcaatcacc
    20881 agaaaaagtc cacataatgg cttatgaaga aaaaataagt gaaaaaaaag gaatagctgt
    20941 taataatttc ggtataggat tagatgcagc tatcgtccat gcgacaaatc attcttccac
    21001 aaaaaagcga ttgaacaaat acaacttagg atcattttcg tatttattct ctattctccg
    21061 tgctttattc actcaaaaag gatttccaat tctggtagat gcaggaggaa aacaattaag
    21121 tttttcaaac gcatttttat gtacagcaac gaagcatcct ttttttggcg gaggaatcgc
    21181 gattgttcca actgctgatg cgtctaaacc agtcattgat tttgtcgtgg tcgaacgaat
    21241 caatttcttt aaaatcttgt ggctcattct tttacttata cagaaaaaac aattgaaatc
    21301 gaaatatttc catcactaca caacaaacag attacgtatc gtttcaacaa caccgcaaca
    21361 tggtcaagaa gatggagaag aaatggaaaa acgacctttt gatatcacaa taaccacaac
    21421 taaacagttg ttttggtgtt aataaaagag gttttgtcag aagctacatc tggaacaccg
    21481 tttattcgga aaaaaggact gtgacacgac ttttgtcaca gtcctttttt tactatgcag
    21541 ttaattcttt gcccaattgg gttgccatcg atagtttctt tctttaccca aaataaattg
    21601 gatcattgaa aacaccgcag caaaagcaac taagacacaa ccattgaacc aaaaagattc
    21661 tgcaaatgtt tgattgtaac tgagtgtttc actgatcaag ttattatttt gaacaaacca
    21721 atatgttggt gtgaatgccg ctattttgct aaccatttct ggcaagtatt ctgctggtac
    21781 aaacacccca ccgataaagc aactgcctgt gataaaaata tctttgatcc caccgctggt
    21841 ttctgagttt tttaccaatg aagtgataca agccgaaaag ctaatgatcg gcaataaaaa
    21901 caagaaagta tttaaaatat gataccatac cgttatatcc cacgaggtgt ggatcactaa
    21961 cagaaccatt agaagtaaga agccactgaa caacagcagc gagtaactaa tcaaactaaa
    22021 ggttactttg cgattcaagt ttcgtttggt aatgggcgca ctctggtttc gtttactgat
    22081 ctctttgcga ttaaatgcta gattgatgac gctgaaaccg ctaaaaatgg atagaaacaa
    22141 cccataagac aagaaattaa aggttgtgcc cgtcaacagc tttttcatcc gctgactata
    22201 tcccgcactt agctcaactt ctccagtaac cgagagtgcc atttgtgtct gttccatagc
    22261 tgcatcgatt gttttatctt cttcttgaaa cagaatcagt gtttgcaaaa aggtgttcac
    22321 ttgctgtgta acaactgttt tagaaaaggc atctgggcga ctctgtatct tgatcgtcgg
    22381 catctttcct acttcgactt gttcggtaaa gtctgcaggc agatgtaaga taatgtttac
    22441 acgattaaaa taaagagcat catcgatttc tttttgtgag gttccatcca atgtaacgac
    22501 tgtttccttg tcattcaaat atttacgaag gccatcgact acaggcgact cttcttcact
    22561 aatcaaggcg attttcgcat cttgcagaga ggtattttca acatcaatat tttgcgcaaa
    22621 aaagaatgtg gcaacaacag tgataattac acctatcagt aaattcgatt tatttgcttt
    22681 aagcaatgtc caaatcgttt tataaagcgt cataacgcac ccctctttcc atccaagcat
    22741 ttcctaacgc aaaaaggaaa ataaatccga tcaaccatag aagattggta taaaaactag
    22801 ccaaatcagt gtagaaataa agttt
//
LOCUS       DAPCMT010000032        22656 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPCMT010000032 DAPCMT010000000
VERSION     DAPCMT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.032"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            37..849
                     /locus_tag="RD743_001050"
     CDS             37..849
                     /locus_tag="RD743_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDU0562878.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            846..1646
                     /locus_tag="RD743_001051"
     CDS             846..1646
                     /locus_tag="RD743_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDU0562879.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1807..3075
                     /gene="rseP"
                     /locus_tag="RD743_001052"
     CDS             1807..3075
                     /gene="rseP"
                     /locus_tag="RD743_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDU0562880.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3143..4852
                     /locus_tag="RD743_001053"
     CDS             3143..4852
                     /locus_tag="RD743_001053"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDU0562881.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5064..9422
                     /locus_tag="RD743_001054"
     CDS             5064..9422
                     /locus_tag="RD743_001054"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDU0562882.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9567..10040
                     /gene="rimP"
                     /locus_tag="RD743_001055"
     CDS             9567..10040
                     /gene="rimP"
                     /locus_tag="RD743_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDU0562883.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10063..11238
                     /gene="nusA"
                     /locus_tag="RD743_001056"
     CDS             10063..11238
                     /gene="nusA"
                     /locus_tag="RD743_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDU0562884.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11260..11553
                     /locus_tag="RD743_001057"
     CDS             11260..11553
                     /locus_tag="RD743_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDU0562885.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11550..11861
                     /locus_tag="RD743_001058"
     CDS             11550..11861
                     /locus_tag="RD743_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDU0562886.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11874..14180
                     /gene="infB"
                     /locus_tag="RD743_001059"
     CDS             11874..14180
                     /gene="infB"
                     /locus_tag="RD743_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDU0562887.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14207..14554
                     /gene="rbfA"
                     /locus_tag="RD743_001060"
     CDS             14207..14554
                     /gene="rbfA"
                     /locus_tag="RD743_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDU0562888.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14711..14884)
                     /locus_tag="RD743_001061"
     CDS             complement(14711..14884)
                     /locus_tag="RD743_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0562889.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15332..17694
                     /locus_tag="RD743_001062"
                     /pseudo
     CDS             15332..17694
                     /locus_tag="RD743_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18062..18985
                     /gene="truB"
                     /locus_tag="RD743_001063"
     CDS             18062..18985
                     /gene="truB"
                     /locus_tag="RD743_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDU0562890.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18989..19930
                     /gene="ribF"
                     /locus_tag="RD743_001064"
     CDS             18989..19930
                     /gene="ribF"
                     /locus_tag="RD743_001064"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDU0562891.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20282..21067
                     /locus_tag="RD743_001065"
     CDS             20282..21067
                     /locus_tag="RD743_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0562892.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21080..21931
                     /locus_tag="RD743_001066"
     CDS             21080..21931
                     /locus_tag="RD743_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDU0562893.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22055..22507)
                     /locus_tag="RD743_001067"
     CDS             complement(22055..22507)
                     /locus_tag="RD743_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0562894.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7684 a         3959 c         4956 g         6057 t
ORIGIN      
        1 gatagtggga atcttttaag tataggagga aaaagaatgt tacgtttttt tccgcaaaaa
       61 aacaaatata ttcaagaaaa aagcgaattt caatttaata ccgatggacc aattcctcag
      121 cacattgcca tcatcatgga cggcaatggt cgttgggcgc aaaaccgtcg tttgccacga
      181 gttgctggcc ataaagaagg catggaaaca gtaaagaaag tcacaaaaaa agcatcaagg
      241 cttggagtga aagtgttgac gctttatgcc ttttcgactg aaaactggaa gcgaccaaaa
      301 gatgaagtaa gttttctcat gcaactacct gtcgacttct ttgatacgtt cgtaccagaa
      361 ttgattaaag aaaatgtcaa agttcatgtc atgggttatg aaaacgtatt gccagaacat
      421 acgcaagatg ctgtaagacg tgcaattgag caaacaaaaa acaatactgg aatggtattg
      481 aattttgctt tgaattatgg aagcagagca gagatcgtga ctgctgtcca agaaattgcc
      541 gaagaagtag caaaaggaga aatacacgca gaagaaatcg atgatgaatt gattgctaag
      601 catttaatga caggtttctt gcctaaagag ttacaagatc ctgagctgat gatccgtaca
      661 agtggagaag agcgaattag taattttctg ttatggcaga tcgcatatag tgagctgtat
      721 tttacaaaag cgctttggcc tgattttgat ggtgcgcatc ttgaagaagc aatcgcctct
      781 tatcaaaatc gagaccgtcg attcggcggt gtaaaaaaaa cagaagatga gaaaggggat
      841 cagtcatgaa acaacgtgtg gtcacagcag ccgtggcgct agtcttgttt attccgatta
      901 tatggatcgg aggcatagcg gttgagctta cagcagcaat cttagcagta ataggagttt
      961 acgagctgtt tcggatgaaa ggattgacac tcttaagttt tgaaggagtt ttatccgccg
     1021 ttggtgcagt gttacttgtt ttgccaaaag aaagatggtt tttctttctt ccagagaatg
     1081 taagtacatt tatgttattt tatcttatag ttatgattct tttaggaaca tcagttattt
     1141 ctaaaaatac gtatacgatt gatgaagcag gttttcctgt gcttgtcagc ttgtatgtgg
     1201 gaataggttt ccagaatttt gtcaatgcac gaacagaaag cttggctgtt ttgatatttg
     1261 gtctatttat tgtttgggca actgatatcg gagcatatat ggttggtcga cgttatggtc
     1321 agcataagtt aagtcctgat atttcgccca acaaaacgat tgaaggtgcg ctcggtggta
     1381 ttggtagtgc tgttgtagtc gcatttcttt atttattgct atacccagca aaagaactat
     1441 ttaatcatgg tatgtttgtc atgcttctga tgacgattat tttttcagca gtcggacaat
     1501 ttggtgattt ggtcgaatct gcaatcaaac gtcattatga tgtgaaagac tcgggaaata
     1561 tcttaccagg ccatggtgga atcttagacc gtttcgacag tttgctgttt gtatttccta
     1621 tcatgcattt atttggcatt atttagtaag tatacctgcg tttctttgtg aatttcataa
     1681 aagacgcagg tttttatttt tcattatatt gatactattg gtgtctttgg tatactatgg
     1741 taaaattata gtgttttatc gttgttttat aaaaaacaca aaaaacaagt aaaggagaaa
     1801 aaagctatga aaacgattct gacatttatc atcgtttttg gtatattagt gattgttcat
     1861 gagtttggtc atttcttctt tgcgaaacga tcaggaatcc tcgttcgcga atttgctatc
     1921 ggtatgggtc cgaaaattta tgggcatcaa gcaaaagacg ggacaacata tacgcttcgc
     1981 ctattaccta ttggcggtta tgttcgaatg gcaggcaatg gggatgatga aacagaaatg
     2041 gcaccaggaa tgcctctttc tcttttacta aattctgacg gcatcgtaga gaagataaat
     2101 ttaagtaaga aaatccaatt gacaaatgct atcccgatgg aattgagccg ttatgattta
     2161 gaagatgagc tgactatcac tggttatgtg aatggagacg aaacagaagt agtaacctac
     2221 cctgttgatc acgatgcgac gattattgaa aacgacggaa cagaaatcag aattgcgcca
     2281 aaagacgtac agttccaatc ggctaaattg tggcagcgta tgctgaccaa ctttgctgga
     2341 cccatgaata actttatttt ggcaatcctg ttattcatca ttctggcatt tatgcaaggt
     2401 ggtgttcaag tgacgaacac taatcgcgta ggagagatca tgcctaatgg agctgcggca
     2461 gaagcaggct taaaagaaaa tgatgaggta gtcagtgtgg acggaaaaga aatccattca
     2521 tggaatgatc tgacaacagt cattacgaaa aatccggata aaacattaga ctttaagatc
     2581 gaacgagaag gtcaggtgca atctgtcgac gtgacaccga aatctgtaga gtcaaatggc
     2641 gaaaaagttg gccaacttgg tataaaagcc ccaatgaata caggttttat ggataagatc
     2701 attggaggga cgcgtcaagc tttcagcggt tcattggaaa ttttcaaagc cttaggatca
     2761 ctatttacag gtttcagttt agataaatta ggcggacctg tcatgatgta ccaattgtct
     2821 tcagaagcag caaatcaagg tgtaacaact gttattagtt tgatggcgct tctttcaatg
     2881 aatctcggaa tcgtcaatct gcttccgatt cctgccttag atggcgggaa attagtcttg
     2941 aatatttttg aagggattcg cgggaaacca ttaagtcaag aaaaagaagg aatattgaca
     3001 ttagctggct ttggattttt aatgttgttg atggtgctag ttacatggaa cgatattcaa
     3061 cgattctttt tctagaaaaa gttgacaaat agggtataat aacaaacgat aactattttg
     3121 aggaaataaa ggagtatata gaatgagaca atcaaaaatg ttgattccaa cattacgcga
     3181 agttccaaat gatgcagaag tattaagcca tcaaatcttg ctaagagctg gatatatccg
     3241 ccaagtatca gcgggtattt attcatatct gccattagca aatcgtgtat tggaaaaact
     3301 gaaacgcatt atgcgggaag aatttgaaaa aatcggagcg gtagaaatgc tgatgcctgc
     3361 catcttgcca gcagaacttt gggaagaatc cgggcgttac gaaacatatg gaccaaactt
     3421 gtatcgtttc aaagaccgca atgatcgaaa aatgatcctt ggaccaactc acgaagaaac
     3481 ttttactgat ttgatgcgta atgaaatcaa ttcatataag aaattacctt taaatcttta
     3541 tcaaatccaa cctaaatatc gtgacgaaaa acgacctcgc ttcggtttgc ttcgcggaag
     3601 agagtttatc atgaaagatg cgtattcttt ccatgcaacg gaagaaagtt tggacgagac
     3661 atataaagat tacgaaaaag cctacaccga agtattcaag cgttgtggct tagaatttcg
     3721 ctcgattatc ggagacggtg gcgcaatggg cgggaaagac tcaaaagaat tcatggcgat
     3781 ctcagaaatc ggggaggata cgatctgtta ttcgactgaa agtgattatg ctgcaaactt
     3841 ggagatggct acaagctact atgtatcaaa aaaatcgcat gaaacacagc ttgaattgga
     3901 gaaagtagct acacctaacg taaaatcaat tgacgaagta gcagatttct tttctgttga
     3961 acctcaaaaa atcatcaagt ctgtattgtt cattgcggat gaacaacctg tccttgtttt
     4021 ggtacgcgga gaccatgaag tcaatgacgt aaaactgaaa aactttttat ctgctgactt
     4081 tttagaagaa gcaacagaag aagaagcaca aaaatatcta ggtgctgagt ttggttctgt
     4141 cggaccagtc ggtgtttcag atgacgtaaa agtatatgca gaccgttatg tccaagattt
     4201 agcgaatgcc attactggag cgaacgaaac aggattccac tataaaaatg tgaatccaga
     4261 acgcgatttc actgtacttt cttatgaaga tcttcgtttt gtacaagaag gagatccttc
     4321 accagacaac aatggtgttc ttgcctttac tcgaggaatc gaaatcggac atatcttcaa
     4381 aatcggcact cgttacagtg aatctatggg agcaactgta ctagacgaaa atggacgaga
     4441 aaaatttgtg attatgggct gttatggtat cggtgtaagt cgtttattat cagcaatcgt
     4501 agaacaacat tcagatgaac aaggaatcaa ctggccaaaa ggaattgcgc catttgattt
     4561 gcatatcgtc caaatgaatc taaaagacga ataccaaaca aacctgacga atgaactaga
     4621 agaagcgatg acaaaagctg gctatgaagt actggttgat gacagaaatg aacgcgcagg
     4681 agtgaaattc gcggattctg atttgattgg ctgtccaatc agaatcactg tcggtaaaaa
     4741 agcagtggat ggaatcgtag aagtgaaaat caagaaaact ggcgaaatgg ttgaagtacg
     4801 taaagaagaa ttggtgaata ctttaccaat cctcttgaac caaggcgaat aataagcgtt
     4861 aaaacacaga aaataaaaat agctgaatca gctgtttttg acaatctgat gattcgcttt
     4921 taaaattgga actgcgattt gaatcgcagt tccaattttt taatgtaaaa ctcagaaaaa
     4981 acgagtatac taaaaggtac tgaaaaagaa gaggataaga tgcgatcaat tgcattgttt
     5041 ctcatcagaa agggcgtata tctttgtcag aaaaacgaga tttatttgaa aaactgttgg
     5101 aacaaatcca actagaggaa gcagaaaaaa atcatccact gcttactgct ggtgaaatgg
     5161 atagtgtagt ggtccatcgt aaatcgcgtt tatgggaatt tacgctgcat ttttccaaaa
     5221 ttttgcctat tatgctatat cggtcattga ctcagcatct gactatcgct tttaaagata
     5281 ttgcacaagt caaactgaat atcttagcag atgaccagac attcgatgaa cagctcttgc
     5341 aagattactg ggcccaagct ttggaaaatc agcaatgcga tacaccacta gcacagcaag
     5401 tattgaaaac gcaggtacct gtgataaaag atcgtaaagt catcttgcca atcgacagta
     5461 caggagctat ttcttatttg aagcaacaat acttgccatt agtagaagag ttgttcgtca
     5521 gttacggatt cccaaaattt cgtatcgaac cagaagtgga tgagcagcaa gcggaacgag
     5581 tattgaaact ttttgaagaa cgcaaacaag aacaggcaga agcctttatg aagcaagcag
     5641 ctgaaagtct agttgtccac gaacaaaaga aaaaggaaag aaaagagcaa tcgccagctc
     5701 ttgaaggacc catccacatc cagctaggac gaaatattcc agccgatgaa ccaacgacac
     5761 caatgatcag tatcgttgaa gaagaacgaa gagtcacact tgaggggtac gtattcgaca
     5821 aagaagtcag agaactacgt tcaaaacgca agatcttgac tttgaaaatc acagattata
     5881 cttcatcatt tatcgtcaaa aaattctcga acggcgaaaa agatgaacaa gtatttgatg
     5941 cgatcagtgt cggtagctgg ttaaaggtac gaggaagtat tcaagaagat acgtttgtcc
     6001 gtgatttggt catgaatgcg caagatatca tagaagtcaa acatacacct cgcaaagatt
     6061 atgctcctga aggggaaaaa cgagtagagc ttcatgttca tagcaatatg agtacgatgg
     6121 atgcaacaaa cagcatctct gatcttgtgg cacaagcagg gaaatgggga caccgagcta
     6181 tcgccattac agaccacggt ggtgcgcaag cctttccgga agcacatagt gctggaaaaa
     6241 aagcaggtgt gaaaatctta tacggcgtgg aagcgaatgt cgtagatgat ggtgtcccaa
     6301 ttgcttataa tgatgcacat gaagcattga gtgaagccac atatgtcgta tttgacgtgg
     6361 aaacgacagg attgtctgca gtatatgaca cgatcatcga gctagccgca gtcaaaatgt
     6421 ataaaggaaa tgtgatcgaa agctttgatg aatttatcga tccaggacat cctttgtcac
     6481 gaacaacggt agatctgact ggtatcacgg atggaatggt tcgaggatca aagtcggaag
     6541 aagaagtatt gcggatgttc cttgaatttt caaaagatac aattttggta gcccacaacg
     6601 cggcttttga tatggggttc ttgaatacta gctacgcaag gtatggtatt cctgaagcgg
     6661 ccaatccagt cattgatacg ttggaattag ctagatatct gtatcctcaa ttcaaacgtt
     6721 tcggattagg tgtgttatct aaaaaatttg gtgtcagctt ggaacagcat caccgagcaa
     6781 tctatgatgc tgaagcaact ggtcacttag catggatctt tgtgaaagag gcaatggaca
     6841 atcataacat gctttatcat gatcagctaa atgagcatat cggagaagga gattcttaca
     6901 aaagggctcg acctttccat gtaacgattt tagcaaaaaa tcaggcagga ctgaaagatt
     6961 tgttcaaact gatttcaatg tcgaatgtcg aatacttcga acgcgtccca cgaataccac
     7021 gctcccaatt gaaaaagatg cgtgaaaatc tattgatcgg ctcagcttgt gataagggag
     7081 aaatattcga agccatgatg caaaaagggg tagaagaggc aagaaaccga gcaaaattct
     7141 acgattatat tgaagttatg ccaaaagcgg tctatgcgcc tttgatcgag caagaattgg
     7201 taaaaaatga acatgatttg gaagaaatta tccaaaattt ggtagaaatc ggaaaaagtt
     7261 tggataaaat cgttgttgca acaggtaatg tacattatct gaacgaagaa gatgccattt
     7321 accgaaaaat tttgatcaat tctatgggcg gagcgaatcc gttgaatcgt catagtttgc
     7381 cggatgtcca tttccgaaca accgatgaga tgctaactgc atttcacttt ttaggggaag
     7441 aaacagcgaa agagatcgta gtagaaaata cgaataaaat cgcagatatc tgcgaagaag
     7501 tgattccagt caaagatgaa ctttatacac cgaagattcc aggttcagaa gatgaaatat
     7561 cagaattgag ttacacaaaa gcaaaacaaa tgtacggaga tccgttacct gagatcattc
     7621 aaaaacgatt aaaaaaagag ttgaattcta ttaatggtaa tggcttctcg gttatttatc
     7681 tgatcgccca aaaactagtg cataaaagta atgaagatgg ctatctagta ggttcacgtg
     7741 gctctgttgg ctctagcttt gttgcgacga tgacaggaat cactgaagtt aatccgttag
     7801 cccctcatta ttattgtcca gagtgccaat attccgagtt ttttgaagat ggtacgtatg
     7861 gttctggatt tgacatgcca gaaaaacaat gtccaaaatg tggtgcacgc ttaaacaaag
     7921 acggtcatga tattccattt gagacatttt taggattcca tggagacaaa gtgcccgata
     7981 ttgatttgaa cttctcaggt gattatcaag cagaagccca taactatacg aaagtattgt
     8041 ttggcgaaga ctatgtttac cgtgccggaa cgattggtac agtcgccgac aaaacagcat
     8101 atggctatgt aaaaggatac gaaagggaca ataatctgca gtttcgaagt gcagaagtcg
     8161 acagattagc aaaaggcgct actggagtca aacggacaac cggccagcat ccagggggga
     8221 tcatcgttat tcccgattat atggatgtct atgattttac gccgatccaa tatccagcag
     8281 acgatcaaaa ttcggaatgg aaaacgactc attttgattt tcactcgatt cacgataatg
     8341 tattgaaatt agatattcta gggcatgatg acccaactgt tattcggatg cttcaagatt
     8401 tatcagggat cgatccacaa acgatcccta cggatgaccc ggaagtgatg cggatatttg
     8461 ccggtcctga agtacttgga gtaagtcagg aacaaattta ttccaaaaca gggacattag
     8521 gtattccaga attcggcact cgttttgttc gagggatgct agaagaaacg catcctacaa
     8581 cttttgcaga actactgcaa atttccggac tatctcatgg tacagacgta tggttaggaa
     8641 atgcggaaga attgattcga cgaggagatg cgacactggc ggaagtaatc ggctgtcgtg
     8701 atgatattat ggtttatttg atccatgctg gattagatag cgggatggca tttaaaatca
     8761 tggaaactgt tcgtaaagga caatggaaca agatccctga tgaattgcga gaaacctatc
     8821 tatcggcgat gaaagaaaat aatgtgcccg actggtatat cgattcttgt tcaaaaatca
     8881 aatatatgtt tccgaaagcc catgctgcag cttacgtatt gatggctctt cgtgtcgctt
     8941 atttcaaggt ttatttccct attttgtatt actgtgcata cttttctgta cgtgcagacg
     9001 actttgatct agtctccatg tgcaaaggaa aagatgctgt gaaacaagca atgaaagaaa
     9061 tcacggacaa aggattagat gcttccgtaa aagaaaagaa tcaactgaca gtgctggagt
     9121 tagccaatga aatgctggaa cgaggattca aatttggaat gattgatttg tataaatcgg
     9181 atgccgttaa ctttgttatt gaaggtgata cgttgatcgc accatttaga gctgtcccaa
     9241 gtttgggaac caacgtggca aaacaaatcg ttgaagcaag aaaagacggt ccatttttat
     9301 ccaaagaaga tcttgccacc cgcggtaaag tttcaaaaac attgatcgaa tatatgaacg
     9361 acaatggcgt attgaaagat ctaccagatg aaaatcaatt atcgttattc gatatgctat
     9421 aagcatctgt tttcctgtct gcttgtaatt ttaagcagag tatggtatag ttcttatagg
     9481 taatgatact aactgtgagt gagcggattt ttcgctcact ctttttaatg gaattacgca
     9541 cttaattttt taaggaggcg aaacttttga gtagcgtcgt tgaaacagtc acagaaatgg
     9601 tgacaccgat tttagatgaa caaaattttg aacttgtcga agtcgaattt gtaaaagaag
     9661 gaaaaagctg gttcttacga gtatttattg acaaagaagg cggtatcgat atcgaagaat
     9721 gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc agcagatcca gatccgattc
     9781 ctcaagctta tttcctggag gtatcctccc cgggagcaga gcggccattg aaaaaagaaa
     9841 gcgactatga aaaggcactt ggtgattatg tccatgtctc cttataccaa ccagtagatg
     9901 gcgaaaaaca atatgaagga tttcttcaat catttgatgc tgaacagctt acattgaaaa
     9961 tacgcataaa aacacgagaa aaagaattga cttttgaccg aaaaaatatt gcaaaagctc
    10021 gcttagctat ccaattttaa tcacggagag gaatcagaaa aaatgagtaa agaaatgtta
    10081 aatgcgttag acgctttaga agctgaaaaa gggatctcaa aagaaatcgt gatcgatgct
    10141 ttagaagcag cattggtttc tgcatacaaa cgtcactatg gccaagcaca aaacgtcgaa
    10201 gtggaatttg atcagaaaaa aggaaagatc catgtctatg cggtaaaaga agtaacggaa
    10261 gaagtgatgg attcacagtt ggaagtctca ttgaaagatg cacttttaat caatccagca
    10321 tatgagatcg gtgatacgat ccgttttgaa gtaacaccaa aagattttgg gcggatcgct
    10381 gcgcaaacgg ctaaacaagt tattttacag cgtgtacgtg aagcagaaag aacgattatt
    10441 tataacgaat tcagtgctta tgaaaaagac atcatgcaag gaatcgtcga acgccaggat
    10501 aaacgttata tttatgtcaa tcttggtaaa attgaggcag ttctttctaa acaagatcaa
    10561 atgccaaatg aattttatca accacatgat cgaatcaaag tttatgtttc ccgagtagaa
    10621 aatacgtcta aaggtccgca agtatttgtt agccgcagcc atccagacct attgcgccgc
    10681 ttatttgagc aagaagtacc agaagtctat gatggaatcg ttgaaattgt cagcatcgcc
    10741 cgtgaagctg gcgatcgttc aaaagtggca gttcgctcaa atgatccaaa tatcgatgca
    10801 gtcggtacat gtgtcgggcc aaagggacaa cgagttcaag caatcgtcaa tgaattaaaa
    10861 ggcgaaaaca tggatatcgt cgaatggaat gaagatccag ctgtattcat tgcaaatgca
    10921 ttgaatcctg cacaagtcat ggatgtgatc ttcgatgaga caaatccaaa ggcctgcaca
    10981 gttgtcgttc cagactacca attatcgcta gcaatcggta aacgcggaca aaacgcacgt
    11041 ttagctgcta aattgactaa ccataaaatc gatatcaaat cagaatctga catgacggaa
    11101 ttttatgaaa atcaagaaca acaaaaagaa acagaagaac tgcatgatga agcaatcgtt
    11161 cagtcagatt taacagacga tgaatatgaa acaattgcat tcaacaatga aacggtcgaa
    11221 gaaaaaccag aagcttaact ttagtaagga ggcaaagaga tgaaacaaag gaaaattcct
    11281 ttacgcaaat ccgtggtttc aggtgaaatg aaacctaaaa aagagatggt ccgaatcacc
    11341 cgttcaaaag aaggggaagt atcgatcgat ccaactggaa aaatgcctgg acgaggagcg
    11401 tatgtctcac ttgagccgga agaagtacaa caagcttggg ataaaaaaat tctcgatcga
    11461 gtactcgaaa caacattaac agatgagttt tatcaagaac ttcttgacta tgtcactcac
    11521 caaaaagcac gtaaagagtt atttggaaaa tgaatacagt taatcggcaa aaagccatga
    11581 atctcatcgg tctagcgatg cgcgcgggaa aactaatcac gggtgaagaa ctgaccattg
    11641 ctgaaatccg caaacaaaaa gcaaaattgg tgtttgtggc aaccgatgca agcgagaata
    11701 caagaaaaaa gataaaagat aagagttcat attatgaagt tccttgcttt gagctatttt
    11761 ctgaagcaga aatcacgcag atgatcggaa aacctcgcaa ggtattcgga ataatggatg
    11821 ccggatttgc gaagaagacc aaggagctaa ttgaaggtta ggaaggtgat tgcatgggca
    11881 acaaaagaat ctatgaatta gcaaaagaac tcaaaaaatc tagtaaagac gtcgtggaaa
    11941 aagcacaaca acttggcatc gatgtgaaaa accatatggg tgcgattact agcaatgatg
    12001 agaagaaatt gcaacaagcg ttcaaaaacc caagcacgaa acaacaagct gcacaacaag
    12061 caagcagaaa accagccaat caacagccga acgatcaacg taaaaaagaa acaacgaaca
    12121 atcaacaaaa aaataaaaat aaccgcagtt atcaagatcg cggtcaaggg agcggtcaag
    12181 tgaatcaagg aaaaaaacca acaacaaacc aaaataatac gggaaatacc agcagccaaa
    12241 atcgtcaagg aacgagccaa ggcggcaatc aaaaccgtac taacaataac aacaaaccaa
    12301 acaataacaa tcgcggcaaa tttaataata ataaccgtaa tcgttataat aaaaaaggta
    12361 aaaaaggcaa acagcaacaa tcaactaaac cagctgtacc accacgcaaa ttccgtgaat
    12421 tgccagatgt attagagtat actgaaggta tgaacgtagc agatattgcg aaaaaaattc
    12481 atcgtgagcc agcagaaatc attaaaaaat tatttatgct tggtgtcatg gtcaatcaaa
    12541 accaagcact ggataaagat acgattgaac tgttagcgac ggactatggc atggaaccac
    12601 aagaaaaagt ccaagttgac attgctgata tcgataaatt cttcgaggca gaagaagtcg
    12661 atccagataa attagtctca cgtccgccag tcgtaacgat tatgggacac gtagaccatg
    12721 ggaaaacaac tttattggat acattacgcc attcacgtgt aacaagcggc gaagccggcg
    12781 gaatcacaca gcatatcggt gcctaccaga tcgatattga tggtaaacca atcactttct
    12841 tggatacacc aggacatgcg gcctttacaa gtatgcgtgc acgtggagca agtatcacag
    12901 atatcacgat cttagttgtt gctgcagatg atggcgtcat gccacaaaca gttgaagcaa
    12961 tcaaccatgc caaagctgca ggcgttccaa tcatcgtcgc tgtaaataaa attgataaac
    13021 caggtgcaaa ccctcaacat gttatgcaag aattaagtga gtatgagtta attcctgaag
    13081 catggggtgg agaaacgatc tttgttgaga tctcagcgaa attcggccaa aacatcgaag
    13141 aattgctgga aatgatcctg ttagtcgcag aagtagaaga tttaaaagca gatccaacac
    13201 aacgcgcaat cggaacagtg atcgaagcac gtcttgataa aggtaaaggt cctgtgtcaa
    13261 ccttacttgt ccaagaagga acacttcatg ttggtgaccc aatcgttgtc ggtaatactt
    13321 acggacgtgt ccgtgtaatg acaaacgatc ttggccgtcg cgataaagaa gcaggacctg
    13381 caacgccagt agaaatcact ggcttgaatg atgtacctca agcaggtgac cgtttcgtag
    13441 tcttcgaaga cgaaaagaca gcacgtgcag cgggtgaaga acgcgcgaaa cgtgcattat
    13501 tagaaaaccg tgctgccaca agccgtgtga ctttggataa cttatttgaa agcctgaaag
    13561 aaggcgaatt gaaagaagtt aacgtaatca tcaaagcaga tgtacaaggt tcagcagaag
    13621 cactggctgc atcattgaag aagattgatg tggaaggtgt ccgtgtaaaa atcgtccatt
    13681 cagctgtcgg agcaatcaat gaaagtgatg taaccttagc agcagccagc aacgctatta
    13741 tcatcggttt caacgttcgt cctacaccgc aggcaaaagt gcaagctgat actgaagaag
    13801 tcgatattcg tcttcaccgt atcatctata aagccatcga agaaatcgaa actgcgatga
    13861 aagggatgct agaccctgaa tttgaagaaa aaatcaccgg tcaaatgatc gttcgtgaaa
    13921 ccttcaaagt ttctaaagtt ggtacgattg caggtgccta tgtaacagaa ggatatattc
    13981 gtagagatag cggtgtccgt gtaatccgtg acggaatcgt aatctatgaa ggccaacttg
    14041 caagtctgaa acgattcaaa gatgacgtga aagaagtcaa aatgggttat gaatgtggtg
    14101 caatgattga aaaattcaat gacatcaaag tagatgacgt aattgaagga tttatcatgg
    14161 aagaagtcaa aaatgactaa aaactgataa aaggaggcca atcattatgg ctaactaccg
    14221 tgaccgccga gtcggtcaag aaatcttaaa agaagtcaat gatattttgc gcaaaaaggt
    14281 ccgtgaccct cgtgtggaga acgtaacgat cacagatgtg aaagttacgg gggacttaca
    14341 gcaagcaacg atctattaca gcattttatc tgacttagca tcagataaga aaacagcaca
    14401 agatggattg aataaagcat caggattgat tcgtcgagag ttagggcaca atctaagtat
    14461 ttataagaca cctgaattgt tctttgaact tgatgaatca gttgtctatg gcaaccatat
    14521 cgatgaattg cttcgcgact tgaacaaaga ctaatcaaaa actcccgcaa atcctgtgaa
    14581 atcaggggtt gcgggatttt tttgttttca ttaagacgta ttacctaaga gttccatgac
    14641 tttagtcttg cggcgtgtca gctaaccaaa aaagagaaaa agaaaagtaa aaaattcgac
    14701 tttctttttt ctagtagtat ataaattttt gcatatagat aaagatcaag ataccgataa
    14761 cggaagctag attgtaatct attttagtga cagtcaggta tagccaagta actagaagat
    14821 cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc gttgacagtt aaaagaaaaa
    14881 tcacgttgaa gttattgata tttccgatta cgccgcttcg atttggtcac gatccaaatt
    14941 ttggataatc cctgtagaaa ttagcatagt agctgggata ccaattcaca tattgacaaa
    15001 gataacagag acttttacat aaaacggatc aatcaggaaa ggaattgctc gatccagccc
    15061 cagttttcca gcaatgtgtt aactggccca gcgtcattca gcaaattatg catgatgagg
    15121 agcgaaatga actgcggaac agccattgtg ataacgacaa gttttttgga tcaatgtaaa
    15181 aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc tgttcatttg gctagatata
    15241 aaatccaaac aaatgaacag aagtgcttat taggtttcaa ttgggagtat ctgcgctaga
    15301 ataagagtaa taatgaaaag gagtgttatg tatggattat tctagtaaag aatactttga
    15361 taaaatgacg gcttggtggc gtgctgctaa ttatctttct gttgggcaac tttatcttaa
    15421 agataacccc ttactgagac gtacactgaa acccgaagat gtcaaaaaac atccaatcgg
    15481 tcattgggga acgattcctg gccaaaattt tatttatgtc catttgaacc gagtcatcaa
    15541 taaatatgat ttgaacatgt tttatattga agggccagga cacggcggac aagtcatggt
    15601 atcgaatgct tatttagatg gaagttacac agagatctat ccagaagtaa cggaagatga
    15661 gacaggaatg caaaaattat tcaaacgatt ttcatttcct ggaggcattg cttctcatgc
    15721 agcaccagaa acaccaggtt ctatccatga aggcggagag cttggatact ctctttcaca
    15781 tgcagtcgga gctgtattag acaatcctga agtaatctct gctgtcgtga ttggtgatgg
    15841 tgaagcagaa accggtcctt tagctggaag ctggttctca aatgtcttta tcaatccagt
    15901 gatagatgga gctgttttac cgatccttca tttaaatggt gcaaaaatag ccaacccaac
    15961 gatcttggca cgtaaatcag atggagaatt agcaaattac tttaacggac ttggctggga
    16021 acctttcttt attgaaggaa atgattcaga aaaattaaat cctgttatgg ctgaaaaaat
    16081 ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa gctcgcctga agacagcaac
    16141 agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc acaccaaaag gatggaccgg
    16201 tccaaaagaa tgggacgggg aaccaattga aggaaccttc cgagcacacc aagtaccgat
    16261 tcctgtagat caagaacata tggatcatgc agatgcttta ctccgttggc tgaaatccta
    16321 tgaaccagaa aaattatttg atgcacaagg acgtatttta gaagaaatca gagaaatcgc
    16381 gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc aatggtggaa tcgatccaag
    16441 accgcttatt atgccagatt ggaaaaaata tactttgcaa tttgaaaaac caggctccgt
    16501 taaagcagaa gatatgaccg aacttggtaa attcgttcgc gagatcattg agaaaaatcc
    16561 agaaaatttc cgtatatttg gaccagatga aacaaaatca aaccgattga atcaagtttt
    16621 caaaacgaca aatcgccaat ggatggaaaa aatcgaacca gaaaatgatg aatggctttc
    16681 gccatctggt cgcgtcatcg acagtcaatt atcggaacac caagatgaag ggttcttaga
    16741 agggtacgtt ctgactggac ggcatggttt tttgcaagtt acgaatcatt cttgcgagtg
    16801 gttgactcta tgttgactca gcacttcaaa tggatgcgga aatcacatga cctttcttgg
    16861 cgaaatgatt atccttcgct aaatctcatt gcaagctcaa cagtcttcca gcaagatcac
    16921 aatggatata gccaccaaga tccaggaata ttgacacatt tggcagaaaa gaaggcggag
    16981 ttcatccgtg aatatctgcc tgcggatgca aatacattgc tcgcagtcat ggataaagca
    17041 ttccgctcgt cagaaaaaat caatttgatc atctcttcca aacatccgcg tgctcaattt
    17101 tactcagcag aagaagcagc cgtcttagtg aatgaaggct tgaaaatcat tgattgggct
    17161 tcgacagcaa aagaggagga acctgaattg gtcattgctg ctgcaggaac ggaatctaac
    17221 ttagaagcat tagcagctgt aactttgctg ctggaagaat tcccaaaact aaaaattcgg
    17281 ttcatcaatg tcgtagattt attgaaatta cgtcatccaa gtcaagatcc gcgtgggttg
    17341 agcgatgaag aatttgatca atattttaca aaagacaaac cgatcttatt tgctttccac
    17401 ggttatgaaa cattggtccg cacgattttc tttgatcgtc ataaccatca tttaatgatt
    17461 catggctaca aagaaaatgg agatatcacg acaccatttg acatgcgtgt agtaaatgaa
    17521 cttgatcgtt atcatttggc aaaagacgca gctttaaaaa tcaaaggaag tcaagcagaa
    17581 gattttgcga aaaaaatgga tcagaaatta caagaacatc aaaactatat tcgtgaaaat
    17641 ggtattgatc tgccagaagt attggattgg aaatggaaaa atcttgacca ataaaatgat
    17701 aaaatcgatc ccgcaaaata gaatttttta cattgattaa gaaactgaaa aaataaataa
    17761 aaaaaacgga aaaaagactg gaagaatggc cagtcttttt tttcgttttt gggaagtgaa
    17821 aaccaaacta tttacctgta agccacccat aaatccaatc aaatggtgca aagaaaaaat
    17881 cttccattct attccctcct ttaaaaaagc ataacatgat ttctatttac gtgagcatga
    17941 attttgttac gtttttattg ccgaaaaata aaaaccgttc attatgatgt caaattcgtc
    18001 agtaattgat gcaatgtgtt ataataattc agttaaatca aaaatgaaat ggagaaaaga
    18061 catggacggg ttattacctt tatggaaaga acgcggaatg acaagtcatg attgcgtatt
    18121 caaattaaga aaaatcttac atacgaaaaa aatcggacat gggggtacgc ttgacccaga
    18181 cgtagacggt gtgttgccca tttgtattgg aaaagccaca aaagttatcg aatatttgac
    18241 agattctggg aaaatctatg taggcgagat aactctaggt ttcagtacaa cgacggaaga
    18301 tcgatccgga gaacgggtag aaacaaaacc agtagatatg tctttatctg aaaaaaaaat
    18361 agatgaagtg atggcttcat ttattggtga gatcacccag ataccaccaa tgtactcagc
    18421 ggtaaaagtc aacggtaagc ggttatatga atatgcccgc aataatgaaa cagtggagcg
    18481 tcctgttaga aaggctcaga tttaccgttt tatccggaca agtgaattga caagagacga
    18541 agtgacagga accatttctt ggcgcttcga agtagaatgc gggaaaggaa cctatgtccg
    18601 cacattagct gtagatacag gcaagaaact tggttaccct gcgcacatgt ctgatctgac
    18661 ccgaacagca agtgccggta tgtcccagaa ggatgctctg acattagaag aagtagctca
    18721 agctgtgaaa aatgggacaa tcgatgattg tttgctgcca atagaaaaag gagtggaacg
    18781 gttcaaacga gtagatatta ctgatgaagt atggcagaaa gtaaaaaacg gtatgagact
    18841 tgattatact gtatttgcgc tcccagaaat gccgtcagaa gaaattgctt tgttttataa
    18901 aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa aaaaacaaat taaaacctag
    18961 taaagtattg agaaatgagg aataacgaat gaatatcatc aagatcagac atccttacca
    19021 atcagaacaa attccagaag acgatgtcgt tttagtgctg ggatttttcg acggcgtgca
    19081 tatcggacac cagaaagtca taaagacagg tatggaaatt gcacaaaaag aaggattgaa
    19141 attagcattg atgactttca atcagcatcc atctattgtc tttaaaaaga tcgatcctag
    19201 cagcgtgaaa tatttgacta gccttaagca aaaagaagaa aaaatggccg ctttgggtat
    19261 agattattta tatgaagttg attttacttc ttcttttgct catttagcac cgcaggaatt
    19321 tgttgatcaa tacatcgtag ggttacatgc aaaatatgct gtttccggtt ttgattatac
    19381 gtatggacca aaagacattg cagatgtcgc tcattttcct gaatatgcca aaggaagatt
    19441 caaaatcgtc acagtttcta aagaagaaga acaaggtgaa aaaatcagct ctacccgtat
    19501 tcgtggatta ctagacagcg gacaagtaaa agaagcagca aaactattag gttgcacata
    19561 tgaggtagaa ggggtagttg tccatggcga tgcacgaggc agactactag gattccctac
    19621 agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa gaaggtgttt atgtgtcgga
    19681 aataaaaatt ggggaaacat ggtatccagc catgggttca attggacata atgatacatt
    19741 cggtgaagga cgggagctga ctgtagagtt gtatattttg gactttaatc aagatatcta
    19801 tggtgaacat gtcgcggtac gctggaatca atttttaaga gatcaagtga aattttctag
    19861 tgtagaggaa ttgattgaac aactcaagga ggatgaacaa gcaacagctg aatatttttc
    19921 tcagcaataa aacattatgc ataaaatctc ttgtttatgt ataactagca attacaataa
    19981 aatgggtgat aaataagccc aggaaagggg ctgtgacaaa agtcttgtca cagccttttt
    20041 ttccaaataa atggtttttc agaagtagct tcttcggaaa taagccgaaa tttcttgaag
    20101 ctgaccactt ctgtcacaac ctctttcttt atagaaataa aaggacacta gcaattcatg
    20161 aaaacgattg ctagagcgta tcatttgctt tagactatat atcttggtgt acactcactt
    20221 taaggcggaa ataaaagtta tccgtaataa caggtttagg tatgaaaaag gagtgggtac
    20281 tatgtacaag gaattagaaa acaaagtcgc agtagtaact ggtggctcaa aggggattgg
    20341 aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca actataactc
    20401 ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg gaaaagcagt
    20461 ctctgtacaa gcagatgtca gttctgaaga aggaatacag cgtttacttg atacggcttt
    20521 agaaaatttt gggacactgg acgtttgggt taataatgct gggatggaaa atcagcatcc
    20581 aacccatgaa cttagccttg aagattggga aagagtgtta aaagtcaatt tgactggcgt
    20641 ttttatcgga acgaaagcgg ctttaaatta ctttacagag aataataaaa aaggaaatat
    20701 catcaatatg tcttctgttc atgaacagat cccatggcca acgtttgctc attacgctgc
    20761 ttcaaaaggc ggggtaaaat tatttaccga aacggtagca atggagtatg caccgaaggg
    20821 aatcagaatc aatagtatag gccctggtgc tatccgtaca cctattaatg cggcaaaatt
    20881 tgatgatcct gaacaaaaag aactcttgga aagtatgatc ccactaggac gcgtaggtga
    20941 accaaaagaa atagcggcga cagctgcatg gttggcttca gacgaatcca gttatgttac
    21001 aggtattaca ctatttgcag atggtgggat gactttatat ccttctttcc aaggtggaaa
    21061 aggataaggg tgaataaaaa tgggtttatt gattgcattg atcccggcag ttggctgggg
    21121 gatccagcca ctgattttga aaaagattgg tggcagacca acaaatgaga tattaggaac
    21181 tggtattgga gcggtgcttg tcggcttact cgttcaatta ttcgcttctc cagctggtat
    21241 ttcagtgact acatttttga ttagtttact ttctggtgca ttttgggtta ttggacaaac
    21301 aggacagtat acatctttca acttgattgg tgtttctaaa acgatgccaa tttcgactgc
    21361 attacagtta gtcggaacct cgttgattgg tgtcttcgct tttggagaat ggtctggaag
    21421 tatggggaaa atcattggag cagcagcaat cattcttctt gtatttggtt ctgctttaac
    21481 ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca aaaggaatta gtctattagc
    21541 atcaacaagt attggttatt gggtatacag tgcattgcct aaactagttg atgcaagcgg
    21601 actttccatt ttctttccgc aaatgatcgg tgtcttttta ggggcagtga tttatgtgat
    21661 tttccgtaaa ccaaccgcat tttctgaaga caagagttgg aaagcaacga tcgttggagc
    21721 catctttagt gtgtcagcat tggcttatat tttttcagca aatgaaaatg gggttgctac
    21781 tgcatatatc attacacagc taaacgtagt tatctctaca ttaggcggta tgtacatcct
    21841 gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt atcggattat tgctgatcgt
    21901 agtaggaagc atggtcacgg tctttatttg aggataaaga tggtaaaatt tcatttatta
    21961 aaaaaatatt tgtttgaacc aaacagtaag tacttataaa aaaatagcgc tagaatttcc
    22021 aacatctctg gaatttctag cgctttttaa ttgtttaaga tacaagggaa atatctgtct
    22081 ggatcaataa tttttcattg atccattgac taaagtttac gacataattg aagttaccac
    22141 cgattagtat ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga cggtcttttt
    22201 tatttgcgta aacatcgtta tggtactgga ctaattgttc tcggaattga ttgattgctg
    22261 taaaatcacg atattgaagg ttgaaacgca tcgtgattgg tgcatttagc atgaatcgct
    22321 gcatttcttt tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa agattacgtt
    22381 cagaaaattt ccattgcccg ccaacctttg ttccttttag ttttccttca tgaagataat
    22441 ttctgataga acgtgtagta acacctaatt gttcggctaa ttcatttact gagtaaaaat
    22501 ctttcaatgt tcaccctcct atgtgaatgg ttctactgac ttagttgata tttgcttaat
    22561 cacgaaatgt agaaagaacg attttaatta aggtcattgt aaaaatcaag tcagaaaatt
    22621 ctaactttta tttctctaaa gaaattcgtt ctctcc
//
LOCUS       DAPCMT010000033        22028 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPCMT010000033 DAPCMT010000000
VERSION     DAPCMT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.033"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(61..1371)
                     /locus_tag="RD743_001068"
     CDS             complement(61..1371)
                     /locus_tag="RD743_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDU0562895.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="RD743_001069"
     CDS             complement(1383..2801)
                     /locus_tag="RD743_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0562896.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="RD743_001070"
     CDS             complement(3041..4609)
                     /locus_tag="RD743_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDU0562897.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="RD743_001071"
     CDS             complement(4613..4894)
                     /locus_tag="RD743_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDU0562898.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="RD743_001072"
     CDS             complement(4891..5814)
                     /locus_tag="RD743_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDU0562899.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="RD743_001073"
     CDS             complement(5811..6689)
                     /locus_tag="RD743_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDU0562900.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="RD743_001074"
     CDS             complement(6676..8013)
                     /locus_tag="RD743_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDU0562901.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="RD743_001075"
     CDS             complement(8016..9119)
                     /locus_tag="RD743_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDU0562902.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="RD743_001076"
     CDS             complement(9193..10701)
                     /locus_tag="RD743_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDU0562903.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="RD743_001077"
     CDS             complement(10667..11359)
                     /locus_tag="RD743_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDU0562904.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="RD743_001078"
     CDS             complement(11381..12355)
                     /locus_tag="RD743_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0562905.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="RD743_001079"
     CDS             12655..13527
                     /locus_tag="RD743_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0562906.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="RD743_001080"
     CDS             complement(14109..16307)
                     /locus_tag="RD743_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0562907.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="RD743_001081"
     CDS             16539..17531
                     /locus_tag="RD743_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0562908.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="RD743_001082"
     CDS             17591..19012
                     /locus_tag="RD743_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDU0562909.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="RD743_001083"
     CDS             19005..20504
                     /locus_tag="RD743_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDU0562910.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="RD743_001084"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="RD743_001084"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDU0562911.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaat
//
LOCUS       DAPCMT010000034        21912 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPCMT010000034 DAPCMT010000000
VERSION     DAPCMT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.034"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="RD743_001085"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="RD743_001085"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDU0562912.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="RD743_001086"
     CDS             complement(1172..1867)
                     /locus_tag="RD743_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDU0562913.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="RD743_001087"
     CDS             complement(1880..2596)
                     /locus_tag="RD743_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDU0562914.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="RD743_001088"
     CDS             2926..3534
                     /locus_tag="RD743_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDU0562915.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="RD743_001089"
     CDS             3859..4182
                     /locus_tag="RD743_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDU0562916.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="RD743_001090"
     CDS             4364..5734
                     /locus_tag="RD743_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDU0562917.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="RD743_001091"
     CDS             complement(5864..7165)
                     /locus_tag="RD743_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDU0562918.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="RD743_001092"
     CDS             complement(7519..9477)
                     /locus_tag="RD743_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDU0562919.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="RD743_001093"
     CDS             complement(9654..9920)
                     /locus_tag="RD743_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDU0562920.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="RD743_001094"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="RD743_001094"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDU0562921.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="RD743_001095"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="RD743_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDU0562922.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="RD743_001096"
     CDS             13264..13827
                     /locus_tag="RD743_001096"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDU0562923.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="RD743_001097"
     CDS             complement(14080..14601)
                     /locus_tag="RD743_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDU0562924.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="RD743_001098"
     CDS             complement(14604..15446)
                     /locus_tag="RD743_001098"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDU0562925.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="RD743_001099"
     CDS             complement(15439..16164)
                     /locus_tag="RD743_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDU0562926.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="RD743_001100"
     CDS             16340..16636
                     /locus_tag="RD743_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562927.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="RD743_001101"
     CDS             17204..18796
                     /locus_tag="RD743_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDU0562928.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="RD743_001102"
     CDS             18809..19549
                     /locus_tag="RD743_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDU0562929.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="RD743_001103"
     CDS             19717..21066
                     /locus_tag="RD743_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0562930.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="RD743_001104"
     CDS             complement(21194..21784)
                     /locus_tag="RD743_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDU0562931.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DAPCMT010000035        21712 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPCMT010000035 DAPCMT010000000
VERSION     DAPCMT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.035"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(205..1104)
                     /locus_tag="RD743_001105"
     CDS             complement(205..1104)
                     /locus_tag="RD743_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDU0562932.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="RD743_001106"
     CDS             complement(1114..1470)
                     /locus_tag="RD743_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDU0562933.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="RD743_001107"
     CDS             1825..2121
                     /locus_tag="RD743_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDU0562934.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="RD743_001108"
     CDS             complement(2765..3718)
                     /locus_tag="RD743_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0562935.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="RD743_001109"
     CDS             complement(3741..4496)
                     /locus_tag="RD743_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0562936.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="RD743_001110"
     CDS             complement(4493..5452)
                     /locus_tag="RD743_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDU0562937.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="RD743_001111"
     CDS             complement(5445..6395)
                     /locus_tag="RD743_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDU0562938.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="RD743_001112"
     CDS             6545..6997
                     /locus_tag="RD743_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDU0562939.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="RD743_001113"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="RD743_001113"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDU0562940.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="RD743_001114"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="RD743_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDU0562941.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="RD743_001115"
     CDS             complement(10549..11358)
                     /locus_tag="RD743_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0562942.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="RD743_001116"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="RD743_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDU0562943.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /locus_tag="RD743_001117"
     CDS             complement(14968..15654)
                     /locus_tag="RD743_001117"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDU0562944.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="RD743_001118"
     CDS             complement(16002..17783)
                     /locus_tag="RD743_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0562945.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="RD743_001119"
     CDS             complement(17809..18723)
                     /locus_tag="RD743_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562946.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /locus_tag="RD743_001120"
     CDS             complement(18750..19712)
                     /locus_tag="RD743_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0562947.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="RD743_001121"
     CDS             complement(19712..20656)
                     /locus_tag="RD743_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562948.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="RD743_001122"
     CDS             complement(20657..21661)
                     /locus_tag="RD743_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0562949.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6062 a         4635 c         3845 g         7170 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa gg
//
LOCUS       DAPCMT010000036        21189 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPCMT010000036 DAPCMT010000000
VERSION     DAPCMT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.036"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..640)
                     /locus_tag="RD743_001123"
     CDS             complement(<1..640)
                     /locus_tag="RD743_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562950.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLY"
     gene            complement(819..1538)
                     /locus_tag="RD743_001124"
     CDS             complement(819..1538)
                     /locus_tag="RD743_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0562951.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1669..2820)
                     /locus_tag="RD743_001125"
     CDS             complement(1669..2820)
                     /locus_tag="RD743_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDU0562952.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3037..3777)
                     /locus_tag="RD743_001126"
     CDS             complement(3037..3777)
                     /locus_tag="RD743_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDU0562953.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3781..4104)
                     /gene="rsfS"
                     /locus_tag="RD743_001127"
     CDS             complement(3781..4104)
                     /gene="rsfS"
                     /locus_tag="RD743_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDU0562954.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4161..4754)
                     /gene="yqeK"
                     /locus_tag="RD743_001128"
     CDS             complement(4161..4754)
                     /gene="yqeK"
                     /locus_tag="RD743_001128"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDU0562955.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4738..5388)
                     /locus_tag="RD743_001129"
     CDS             complement(4738..5388)
                     /locus_tag="RD743_001129"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDU0562956.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5398..5709)
                     /gene="yhbY"
                     /locus_tag="RD743_001130"
     CDS             complement(5398..5709)
                     /gene="yhbY"
                     /locus_tag="RD743_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDU0562957.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5725..6834)
                     /gene="yqeH"
                     /locus_tag="RD743_001131"
     CDS             complement(5725..6834)
                     /gene="yqeH"
                     /locus_tag="RD743_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDU0562958.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6831..7358)
                     /locus_tag="RD743_001132"
     CDS             complement(6831..7358)
                     /locus_tag="RD743_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDU0562959.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7538..8323)
                     /locus_tag="RD743_001133"
     CDS             complement(7538..8323)
                     /locus_tag="RD743_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDU0562960.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8316..9185)
                     /locus_tag="RD743_001134"
     CDS             complement(8316..9185)
                     /locus_tag="RD743_001134"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDU0562961.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9186..10565)
                     /locus_tag="RD743_001135"
     CDS             complement(9186..10565)
                     /locus_tag="RD743_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDU0562962.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10573..10992)
                     /gene="fabZ"
                     /locus_tag="RD743_001136"
     CDS             complement(10573..10992)
                     /gene="fabZ"
                     /locus_tag="RD743_001136"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDU0562963.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11017..11493)
                     /gene="accB"
                     /locus_tag="RD743_001137"
     CDS             complement(11017..11493)
                     /gene="accB"
                     /locus_tag="RD743_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDU0562964.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11496..12731)
                     /gene="fabF"
                     /locus_tag="RD743_001138"
     CDS             complement(11496..12731)
                     /gene="fabF"
                     /locus_tag="RD743_001138"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDU0562965.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12750..13487)
                     /gene="fabG"
                     /locus_tag="RD743_001139"
     CDS             complement(12750..13487)
                     /gene="fabG"
                     /locus_tag="RD743_001139"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDU0562966.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13499..14416)
                     /gene="fabD"
                     /locus_tag="RD743_001140"
     CDS             complement(13499..14416)
                     /gene="fabD"
                     /locus_tag="RD743_001140"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDU0562967.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14523..14747)
                     /locus_tag="RD743_001141"
     CDS             complement(14523..14747)
                     /locus_tag="RD743_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDU0562968.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14800..15762)
                     /locus_tag="RD743_001142"
     CDS             complement(14800..15762)
                     /locus_tag="RD743_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDU0562969.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15772..16212)
                     /locus_tag="RD743_001143"
     CDS             complement(15772..16212)
                     /locus_tag="RD743_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0562970.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            16518..17483
                     /locus_tag="RD743_001144"
     CDS             16518..17483
                     /locus_tag="RD743_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDU0562971.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(17540..17770)
                     /locus_tag="RD743_001145"
     CDS             complement(17540..17770)
                     /locus_tag="RD743_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDU0562972.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17856..18743)
                     /locus_tag="RD743_001146"
     CDS             complement(17856..18743)
                     /locus_tag="RD743_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDU0562973.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18832..20055)
                     /locus_tag="RD743_001147"
     CDS             complement(18832..20055)
                     /locus_tag="RD743_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDU0562974.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(20072..21118)
                     /locus_tag="RD743_001148"
     CDS             complement(20072..21118)
                     /locus_tag="RD743_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDU0562975.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         5735 a         4702 c         3589 g         7163 t
ORIGIN      
        1 tatataaatc caaatctgtt tcaggatatt ttaatttgac aagcgaaata atcattttca
       61 gttcttccat gttttctgga tattctgggt caaatacctt gctatgacgg ccaatcccgc
      121 cattgtcgat caatcggtaa attcgattcc attcaatgaa ttggtcaatt gattggaaat
      181 tgctgattgg atagccgcca caatccaaaa tctctgatac acgaaatgct aggtatgcac
      241 tatcgatcac tttatttttt ttcgtgtcta tcttctcatt tctttcgata agaatttttt
      301 caggacgatg tttcaagctc cactctaata gtagaaagaa agcctccatc cgatactctt
      361 cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc agataatttt
      421 gtctataaat cagcattggt gcgcggtcca ccatacactc taaataacgg ttcagatacc
      481 ctgtaccgac atagtttcct aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg
      541 ctaattttga acaatcagtt acgttcacag ctaactcttc tactttgcgg taaccgctta
      601 agatacgttc aaaaggttct aaagtgattt ttgcttccat attcttcccc gtccatcttc
      661 cacatacttc atatcatttc acatgatact tatgctctca tcataccaca ggttgaatta
      721 gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt
      781 tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa tgccacatta
      841 tggaacacat gctgtacatc ttctagatct tccagtgcat caatcatttt ttcaaatgtt
      901 tcttgatctt ctggagatag tgttacctcg ttttgaggga tcatttggat ctcagcgatt
      961 gaaaaatcag tgattccttt ttcttttaat gcttcttgta tactatggaa atccgctact
     1021 tcaccgtaaa cgatgatttg tccttcttct tccattacat cgcgaacatc gatgtctttt
     1081 tccattaggt attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta
     1141 ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt tcccgccatt tttcccaaat
     1201 gctgcccgta cgtctgctgc tgttcgattc acattgttcg tcaatgtatc aacaatcacc
     1261 attgagccgc ttggtccaaa tccttcatag cgcaattcag agtatgtttc atctcctgca
     1321 ccttttgctt tttcgattgc tcggtcaata atatgttttg gtacattata agttttagcg
     1381 cgatcaatga cgaaacgtag tttttggttt gcatgcggat caggatcacc tgattttgct
     1441 gccgcgtaga tttcaatccc aaattttgca taaatccggc tattattagc atctttagca
     1501 gttttctttg ctacgatatt tgcccattta cgtcccatat tttcacttcg ctttctgatt
     1561 tgatagtttc ataacgcaaa aacgtcctgt tccattatac ttgtgaatca cgattctgtg
     1621 aagctttttt tctttcttcc ttcattcttc tcatttttag tcaattatct atactcggat
     1681 tgggatttgc ccgaaattct gttccggaat attgcctccc aattgataga tttgatcact
     1741 tctaactaac agttccgctt cttgtgcctc tttttttccg attcgggaat aaatagaaat
     1801 ctcttttttt tcttttaaga atcttcttcc agcatcggta aatccaagta gtcgaagata
     1861 atttttattt tgttggacag gaatctcttg ctttttgata ttcagcaaga cataacaaga
     1921 taatcgttgg atccgtgtcc atgtatatct ttttgttttt acctttgaca caaaatcctc
     1981 aaaactatca gcagttgcag ctgctgtttt catgcgcgat tctattcctt cggtcatctg
     2041 atagatatct gtcagttcca gcaaagtaga agataacaac cgataacgca aatacggcca
     2101 ataagattcc caagtctgca gcgaacaagt tgaaagatct tcttctgtct gaggcggcac
     2161 aagtccagct attctttttc cttcttctaa cgcatggcga atggctgttg cactggctat
     2221 cctctgatct gtgatgattt tttcatgata gcctgcactt ttacgtcgga tcggatgtaa
     2281 ttccataggg tggacatact tggcattttc ctttgcataa cttaatccca agatatgatt
     2341 tggcgaagaa aaatcaagcg taatatcggg atacagactg cgaaaaacct cagtcatttt
     2401 ttgcggataa ctatacgttt gatcagacag ttcttgaaat gcctcttcta tcctatcttg
     2461 attctcttgg acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt cttctgttcc
     2521 aaaacacaaa ctgtcacagt ccagtgtttg caagattttt actgcacctt tagcaaaata
     2581 gtcagctgac tgaaccgccc attcgacggg aagctccact actaaatcca caccattttg
     2641 taaagcttca ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa
     2701 attcccgctc attacagcaa tgacgacctc tgctcctgtt atttttcgtg ccatttccac
     2761 atgatagcga tgtccattgt gaaaaggatt gtactctacg ataatcccgc agcttttcat
     2821 gcgcatcctc cttcatagtt atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa
     2881 aatcggaaaa aatacccgaa ctatataaac tattctcgca aacacaatac ttctgcgacg
     2941 aaaagctgca ctgcaagagc aatgcctata tcgttcggat atataattta tgttgaagat
     3001 ttattctttt cttgttaact cgagcaggca aaaccgctag tctttgtatg ctacaaagaa
     3061 ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt
     3121 cgaaaagccg ctgttttcca gcattcttaa ataactttcc aatggatagg tacgctcttg
     3181 atgtagttca tcttcacgaa taaatcgacc agacccttct tgttcttcaa cgaaaaaggt
     3241 aagaaaatgt tcgatgctgt gtttctcgct tcctggataa ctatcccaca aaaaagcaaa
     3301 attatcccct tgataatgat agctatattc tggaaaaacc tcatctactt gataggtgga
     3361 gtgtacgtca aataaaaatc ggccattttc ttctaacgct tgatagactt catcaaacac
     3421 ttgctgtacc tcttgtctat ttttcatata gcacagcgaa tcagaaaaac aggtaattgc
     3481 ctgatattgt ccgatttctg ataaatcgag catattgcct tccacaaatt ggatcgatgc
     3541 gtcttcttca tatgctcgct ggctggcaat catcaacatc tcttctgaaa ggtctaaccc
     3601 tgtcacttta tatccatctt ttgcaaaggc aaccgctaat gctccagtac cgcatgccag
     3661 ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta accattgctg
     3721 ataaagtgtt tcgtccatca cttcatcgta aatgaaagca aatgtttcat aagccatatg
     3781 ttaatccaac cattcactaa gatcgactag cggagcatct gaccatagtt tttccagatt
     3841 atagaaattt cgttctggcg catggaaaac atgtacgatc acatctccaa gatcgatcaa
     3901 tacccatttt ccgccttctt ttccttcaat acgttttact tcatatttgt tttcttcttc
     3961 tttatcaatg atttcttctg taattgcgtt gatttgacgt tcgctatttg ctgaacagat
     4021 catgaaataa tctgccaaca aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc
     4081 ccgttttgaa tctgctgctt caatcgcaat ttttaattgt tctttactat ctatgatgat
     4141 ttcctcctaa tatgagcagc ttattttttt gctacccagt aattatatgt ttcgattgtt
     4201 tttggataaa tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat
     4261 gctacagctt cgtctagatc agcccatgca atggctcgtg catcttgtac cccaggaaaa
     4321 tctcgacctg gttcaatata gtctgctaca taaatgattt tgtcaaggag actcatctta
     4381 gcagccccag tcgtatgcac ccggattgca tgaagaatct ctgcatcggt gattcctaat
     4441 tcccgctcta caaacgaagc gccaacaaga ccatgccaga ttgcattatt ataattcaat
     4501 aactccggat caaaaccgtc tcttacgatt accattttga actcctcatc tggtcgttct
     4561 tttgcataat catgtgtcaa tgcagcaata ctagcttttt caggagaagc accgtatttt
     4621 ttggcaagag ccacggctgt ttcttctact ccaagcacat gcttgaagcg ttgttcgctc
     4681 attctcatct gtattttttg catcaggttt tctctagaat aagcagtata ttcattacta
     4741 tagtccatat tcatacaacc cttcattttg aatataatca attactttat ctggaacaag
     4801 gtagcggata gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat
     4861 ctcaggtaca tctacccaaa ttacaggata tggagtatcc gttgtgtatc ctggtcgacg
     4921 tatacccaca aagttgacca tagaagtcag ctcatcgatt ttataccatt ttggcaaata
     4981 ttctaccata tccccaccga taataaagta ataatctgta tccggattgt tttgcgtcaa
     5041 ttccttcata gtatcatagg tgtaactttt cccaccacga gctaattcaa tcgtttcaat
     5101 ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc
     5161 aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc
     5221 aagacctaaa ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt
     5281 gaatgttccg cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc
     5341 aggaaataat tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta
     5401 tattgctttt acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa
     5461 tacaataaca cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg
     5521 agctgcttct tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct
     5581 tttttctaaa gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat
     5641 ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct
     5701 taaactcata attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc
     5761 ctttggcgcc cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc
     5821 aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc
     5881 ctcgacttca tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc
     5941 gtctgctttt tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa
     6001 tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg
     6061 cccttcattc agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa
     6121 atctttttta cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat
     6181 caaaaaatgc ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg
     6241 cgaagttgtg attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt
     6301 cgattttccg acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc
     6361 gattttttct aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc
     6421 tactggacga attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt
     6481 tttcaatgat ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc
     6541 gacaaaacgg tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt
     6601 gacgatcaat gcatcttttt gccctaattc attcagtaag cgcaaaaagt cgtcatccgt
     6661 caatgagaca tcttgggtct cattataatg gcgtaatctg aaacaacgct gacaatagac
     6721 ttccccggaa gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg
     6781 tttgtttgtt tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt
     6841 ccacctcgcc agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc
     6901 caaaaacgat taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg
     6961 ctacgtatcc ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt
     7021 actatctcat catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa
     7081 ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt
     7141 tttgaattat tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc
     7201 caagtaagca actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa
     7261 tcggtcaata ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg
     7321 gcttctacca tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa
     7381 atgaaggagc acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa
     7441 ctacctctat actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata
     7501 aagattgatc acagaaaaat ttagaaacaa aaaacaacta atattggcgg aacctcttgt
     7561 atcgtttttc cagtaattta tgctgatcta atcgaacaag ttccgtcaat ctttcaatca
     7621 acgctttttt cataatgtgt gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt
     7681 caggaataac tcgatcaacg attcctaact cgcgtagttc tggcgcagta attttcatca
     7741 actcagctgc ttctgcggcg cgactaccgt ctttccacaa aatcgacgca aatccttctg
     7801 gagaaagcac agcataaatc gtttcttcca gcatccagac ttcatccgct aaacctagag
     7861 caagggcacc gccacttcca ccttcaccaa tgatgatggc gatgatcggt acctttaaat
     7921 cagacatttc gagcagattt ctggcaatag cctctccttc gccgcgctct tctgctccta
     7981 caccgcaata tgcacctgct gtattgatca aggtaatcac cggtctattg aacttttccg
     8041 cttgtttcat cagtcgtaaa gcttttcgat agccttcagg atggggagaa ccaaaatttc
     8101 tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt accggtttgc
     8161 catctaatcg agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat
     8221 ggcattcctc gaaagaatcg aagatctctt caaaaaaatc caagcttgtc catcgttctt
     8281 tagctcttgc taactgaacg acatcatgtg ctgtcttatc cacgatctat ccatcctttc
     8341 tgtgtatgca attcaagaag cgtatggatt ttctgtttca attccatgcg gggaacaatc
     8401 tgatcgacaa aaccatgaga taataagaat tcagcctttt ggaaatcttc tggaagttcc
     8461 tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct ttggggttct
     8521 gctagaataa tatcgccttc cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc
     8581 gttaaaacag ttagataaaa aagaccttca ttgctatgcc gtttgacagc agcagagatt
     8641 tttgccatct gcattagaga aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg
     8701 aacaagacga ctggaagact ttcctttgtt gctcgttcga ataatctggt gatcttttct
     8761 ccgacaatcg ttcccatact tcccatgata aaattaggat ccatcactcc aataatgaag
     8821 ggaatcccct gaatggttgc tttaccagtg aggactgctt catgaagtcc tgttttatct
     8881 tgcatttttt taattttttc cgtatattcg ggaaatgcta gcggatcttt ggttacaaga
     8941 tccgtatccc attcttcaaa acttttttca tcaataatca aagctagacg ctgccatgca
     9001 ccgattcgaa aagcgtatcc gcaatgaggg catacttttt cttcaccgat gtctttcgta
     9061 taaaggatat gtttgcaatt tggacacttt gcccacatgt tatcaggaac agatggcgag
     9121 ttagcaggtt tgctctctct attaggattg atccgaatat atttcttttt cttaaacaga
     9181 gccatctatg cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt
     9241 aaaaatgctg tgctgtaatc acctgctatg actgcaggat gactaatcaa atccatctga
     9301 aattctgcgt tcgtgatgat tccttctgtg ataaattcac ttaaggctcg ctgcatcttc
     9361 attaatgctt caaaacgcgt atttccatgg acgattattt ttgcaatcat tgaatcataa
     9421 tatggcggga tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc catcccacct
     9481 gctggaagca gtaagttttg gatcttccct ggagaaggag caaaatggaa agcaggattt
     9541 tctgcattaa tccggcattc gattgcgtgt ccttggaata ctacttcttc ttgacacaca
     9601 ttcaaaggct caccggctgc tatctccacc tgttttttta ctaaatcgat tcctgtgacc
     9661 atttctgtta ctggatgttc cacttggatt cgagtattca tttccatgaa ataaaaatcg
     9721 ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt cataattgac tgctttagct
     9781 gctcgaacag cagcgtctcc tagtgcttct ctttttgttt gtgagatcac tacagaagga
     9841 gattcttcca gtactttttg attgttgcgt tgtaaagaac aatctcgctc gcctaaatga
     9901 atgacatgac caaatcgatc acctaagatc tgaacttcta tatggcgagc aggataaatg
     9961 attttttcca aatacatatc atcatttcca aatgccgctg ctgcttcttg ctgagctgaa
    10021 gaaaaatgct gtgggagctc ttcttttgat tgaactttcc gaatgccttt cccgccacca
    10081 cctgcagctg ctttcagcat cactggataa ccaattctgt ctgctatttc caatgcttct
    10141 tctactgtag ataatacacc agtacttcct ggaatgactg ggacatttgc ttcttgcatc
    10201 aattgacgcg cattgatctt atttcccatt gcatcgatcg ttgcactttt aggaccaata
    10261 aatgtgatat tgcactcttc acacatggag gcaaatcggc tattttcaga taaaaaacca
    10321 aatcctggat gaattgcttc ggcttttgtc acgattgcag cgctcagtac ttcctgaaca
    10381 ttcagatatg aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt
    10441 gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta cggtttggat ccctagttcg
    10501 cggcatgcac gaatgatacg tacagcgatt tcaccgcggt tagctatcaa tactttagaa
    10561 aacatatttc acctatccaa tcataaaggt taattccgct tcggctactt ttttaccgtc
    10621 tactttagcg attcctttgc ctattccggc tgaagctttc actttcaata tctcaacttc
    10681 aaggtataag gtatctcccg gtgtaacttt cttacggaat tttgctttgt ccaatccgcc
    10741 gaaataagcc gtctttccct tgaattgttc aagagaaagt aatgcaactg ctcctgcttg
    10801 agccattgct tcaacaatca atacccctgg cataacaggt tcttctggaa aatgtccttg
    10861 aaaaaagggc tcattgatcg ttacattttt cttagcaacg atccgttctc cttcaaccat
    10921 ttcttccact cgatcaatca aaagcattgg ataacgatgt ggaatgattt cttttatttc
    10981 ttgtatgttc attgttttcc ctctttcctg ctttgcttat ttttgctgaa tcctaaagag
    11041 tggctgtcca tattcgacaa ctgactcgtt ttctacaagg atttctgtga tcactccgtc
    11101 ggcctcacta gttatctcat tcatcagttt catcgcttca ataatgcaga taacttctcc
    11161 tttggcaact tgatcaccta cttttttgaa agctggtttg tcgggcgctg gttgcagata
    11221 aacgattccc acgattggcg aaacaacctc gattccatca aatactgccg attctttttc
    11281 tttcattatt tctggagcag ttgttgcttg ttcaggtatg acattgactg gctccgaaac
    11341 tggcaacgtt tcgtttgttt gaaccatatt catgcctcga cttgttttat ttttattcat
    11401 atacaattca aattgacctt cacgcaaatc gaattccgtt agcgaggatt gatcaaattg
    11461 actgactaat tctctgattt cattgatatc caaaattatt cctcccagcg tttcatgcac
    11521 agaaccgcat tatgtccgcc aaaccctaat gaattactca ttgcataagt aaaacttgca
    11581 ggtttgcttt cgtttttgac aactgtcaag tcaatctcag gatcttgttt ttctacattg
    11641 atcgtaggtg ggataaattg atgttctaat gccagcaaag ttgccaaggc ttcgatcccg
    11701 ccggctgctc ccaataaatg tccagtcatc gatttcgtgc tgctgacgta agtatctctg
    11761 aaattttcac ctaatgcgat ttggattgct tttgcttctg cctggtcatt ggctggtgta
    11821 cttgttccgt gagcattgat atatccgact tgttccggct gtacaccagc ttcttccatc
    11881 gcaagcttca ttgctttaga tgcaccagaa ccatctggag taggagctgt catatggtaa
    11941 gcgtcacagt ttgcaccgta tccaacgatt tctccataga ttttggcacc gcgagcttgt
    12001 gcatgttcta aagattcaag aactagaacg cctgctcctt cacccatcac aaaaccattc
    12061 cgattttcgt caaacggaat cgaaccacga ttaggatcag ttgattctga gagtgctgtt
    12121 aaagctgcaa atccagcaat ccccatctca caaatcgttg cttctgttcc tccagcaaga
    12181 atgacatcag aatagccatg tttgatgtta cggaatgctt ccccaattga attcgttccg
    12241 ctagcacaag ctgttacgat agctgtacaa ataccttttg cacctacacg caaagcaata
    12301 tttccagctg ccatgttggc aatcgccatt ggtacgaaca ttggagaaac tcgttttgca
    12361 cctttttcat gtagacgaat aacttggttt tcgattgttg gaagaccacc gatcccagat
    12421 ccgatgatga cgccaaaccg gtcttgattc attttttctt tatctaaacc gctcatctcc
    12481 attgcttcta aagcagcgta gattccatag atcgaaaaca tatccattcg tttgctatct
    12541 tttttaataa aatatttatc gtaagggaaa tctttgactt cacccgctaa ggtaatccct
    12601 gtttctgaag catcaaattt agtgatcggt gcgatgccat tcgtcccctc ttttaagcta
    12661 ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga ctccatagcc agtaattacc
    12721 actcgattca tttgtttgct ctccttttct tatccgtgca tgaccaatcc gccatcgaca
    12781 ttgatgactt gtccggtgat atatggattt tgtgctaaga aaactgctgt ttgtgcaata
    12841 tcttctactt gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc cttgactttg
    12901 tctgctaaaa cttccgtcat atcagttgtg atgaatcctg gcgcaatggc attacatgta
    12961 atgcctctag ttgcagcttc tcgtgccaca gattttgtta atcctactac gccagcttta
    13021 ctagctgcat aatttgcttg accaatattt ccaatcaacc cagatacact agagagatta
    13081 atgatggccc cttctcgctg cttcatcatt tttttcaata cgtgctgcgt catattgaag
    13141 gttccaatca aattgatatc cagacatttt ttgaaatctt ctgcatccat tcgcatcaca
    13201 agtttgtcat ttgtgattcc ggcattgttt actaacacat ggatcgatcc aagcttttct
    13261 tctgcttctt tgatcatttg tcccgctttc tcataatcag agatgtcacc ggaaactcct
    13321 acgcatttta caccgaatgc ttcgatttct tctatctttt cttttggaat ctctccgcga
    13381 ccattcaaaa tgatatttgc tccagctttt gcaaaagcta aggccatcgc tttgccgatt
    13441 cctcttgtac tacctgtgat aaatacattt tttcctttta catccataac gttcctcctt
    13501 atagtattgc tattgtttca tcaaatgtct gcttattctc aacacgcaga actggtattg
    13561 ttttatcgat ttttttcata aatccgctta atacttttcc ggggccgact tcgatgactt
    13621 gttcgatgcc gattgttttt aacttatgga tactctcata gaaacgaaca ggttgcatga
    13681 cttgctgttc aagtagtccc gcgattgttt ctttcttcat tatttccgtt gttgtgttgc
    13741 ttatgatagg aaaagaaggt tcagagaatt gaatctgact taggtccttc gccagttttt
    13801 ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct gacatttaga gggatcattc
    13861 ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc tactgctttt tcttcgccac
    13921 caatcacgat ctgctgaggt gtattataat ttgcaggaga tacgatgccg tacttgctag
    13981 cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt catcactgca accattttcc
    14041 cgcttccagc tggggcagct tctgtcatat aagcgcctct ttttgctact aacgcaactg
    14101 cttcagtgaa ggatagcgca ttactagcta ctaatgccga atattctcct aaactcagtc
    14161 ctaaagctgc atctgggatg attccatgct ccttcaataa gcgataatac gcgatgctga
    14221 ctgttagaat agctggctgt gtatattgcg tctgatccaa acgttcattt tctgtaaagc
    14281 aaagttcagc catatcgtag ccgagaatct cacttgcttc atcaaatgtc tgctttacaa
    14341 cggcttcttc atataaatct tttcccatac cttgatattg tgcaccttgg ccactaaata
    14401 agaatgctgt tttcatgttt ttctccaata tatccagaat atatgtttga ttcttcctat
    14461 tcaaaaaggc cgagacattc ctatttcagg aaatgtccca gcccccaacc taattttaat
    14521 gattatgcag tttgtttttc tacgtaagtc actaaatctt gaaccgtttt gattccgtct
    14581 tctgtttcga ttttcacgtc aaattcatct tcaatttcat tgatgatttg gaataaatcc
    14641 aagctgtcag cttctaagtc ttcttggatg tcagtagtca atttgatttc ttcagtttct
    14701 tttcctaatt cttctgcgat gatttcttga attttttcga ataccataat aatatattcc
    14761 tccgatattg tttgcttttt atttttataa tttaacagtc tacaactcca tgagaatact
    14821 tccccatgtt aaacctccgc catagcctgt aaatattact ttctgctgtt ttcctaaggt
    14881 gatctttcca ctagaaactg cttcatccaa taagataggg atgcttgcag cagatgtatt
    14941 cccatattta tccatattgg tcaggaactt ttcttgcggc atctttactt ttcgggcaat
    15001 tttctcaata attctgaggt ttgcctgatg caaaagcaga taatcaactg gtttgtcttt
    15061 catgacttct tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc
    15121 agtcatttgc aaataatacg aaccttctga atcttctgaa tagaacggat ttttattttg
    15181 ctggtagcca gcggtcaatg agctacttct ggtaccgtca gctgctaatt tttctttcag
    15241 caattttttc tgtggattcg cttctaagag aatcccacct gctccatcgc caaacaacac
    15301 agcagtcgta cgatcagacc aatcgattag cttagatagt gtttcaccac caatcaccag
    15361 tcccttagtt gagccgcagc ggatcatttt ttcagccatg cttaacgcat aaacaaatcc
    15421 tgagcacgca gcactgatat caaaagcgat agcatgagtt gctcccaatt tcccttgtac
    15481 ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt gttgctacta aaataaaatc
    15541 caaatcttta gggtttagat ttttctgttt tatcaatttt tccgccacta aaatgcataa
    15601 atcagaagtc tcctgatttg ttacacatct tcgttcacga atacccgtcc ttgacgaaat
    15661 ccattcatca gaggtatcca tcatcttgga aagatcatta ttagtaataa tcttttcagg
    15721 aaggacgctg gctgttgcag taatcattcc aaattgttcc attaaatatc ctcacttata
    15781 gtcttgcaag aagtcgtgca agtttttcag tgcatccagc aaggcatgct gttcctccgt
    15841 agccattccg tctaagatgt tcttgaccat ttcacgatga aaatgctgat ggacccgaaa
    15901 tagtaacttt ccttttttcg ttagtcccaa ctttacaacg cggcggtcat cttcactgcg
    15961 gatacgttcg acatagcctt tttttactaa cttgttaata gcagtggtca aggtcccaac
    16021 agtaattgat agttctttgg ctacttctga agttgttttt ttcttgtaca tcccgattgc
    16081 ctcgatcgtg tgcatctctg tgattgacag atctttgaat tgagattttt tcagctcaga
    16141 ctcttcgatt gtcaaaatat cattgaagac actaacaaga taatcattta ctgtttttaa
    16201 atctggttcc atgttccacc gtccactaat tagtttgatt atcaaatctt ttgatgttca
    16261 aactatatct taactttttt caaaatgcaa ccttaaaaga tcattttttt actatatgag
    16321 ctttttttaa gattttactc atttaaaaca actgataaat cagcatttat aatttctttt
    16381 attagaaaaa tcatctatct cacatatttt gattatcaaa gtattttctg tgaacagaac
    16441 tatagaattc cctaaaactt gtccattatt tcaaaatttg ttatagtctt tttattcaaa
    16501 gcgtaaaagg agatcctatg ttaaaagaaa aaatgaaaac tctccccatg ctgccgggtg
    16561 tctatcttat gaaagaccga gatggtaagg tcatctatgt tggtaaagca aaacggctca
    16621 aaaatagagt aagcagctat tttcatcaga ataaacagca ttcaaaaaaa gtattacgta
    16681 tgattcatca tataacagat tttgatttcg tcgtagtaga cacggaatta gatgctttat
    16741 tgctcgagtg tcaattgatc caacattacc gtccttttta taaccgacaa atgaattatt
    16801 tcagtaatta taattatgta catattacca ataagggatt tgtattaaca gatactccta
    16861 cggctcgaac atacgggcct tttcgtctct ataaaaaaat gccgtctatt ttgcgtataa
    16921 tggaagaaac ttatcaaatg ccttggttgt ctgaaattag tttgttagct ttgcgagtcc
    16981 agcttcctga cctacaggaa atgacttttg aacagaaaaa aaaggaactc caagggctgt
    17041 ttcaaggccg aaataaaaag ctactgactt acttgaaaaa aagacaacaa catttcatct
    17101 atcagttgaa tttcgaaaaa gccggtatgc ttcaaaaaga tatcgaatta gttacttatt
    17161 ttattagacg gatccaggaa caaaagcagt ttttaagaac accatcgctc accttcagca
    17221 tgccattagc agcagatgag agtcagaaaa agcactacct cgtttgttat ggtcaacttg
    17281 cagaaacaat gattacctct ggtgacgttt ctcctgactt ctattatgaa aagaaagaag
    17341 ctcatttatc tttgaaacgt caattgtcaa aagaagaaat cgatcctgta caaatcttga
    17401 tcagttaccg caaaaaatta gaaaaagaac agtttgaaat tgaactgtta aacaaaaaag
    17461 aagctgaaaa gcaactaaat tagttgcttc tcggcttctt ttaggcatct tcaacttcaa
    17521 atgtcaatta tcctaaaatt tattggatta agtcatagat ttccatcgct acgatatcga
    17581 tattatcaaa ttcgtattgc tgatgagtat ctgtttcagt taattcaaat gtatcggtcg
    17641 ctttatcata agttactgta cacttctcta cgccttcttt ttcaaaacgg cgaacttgca
    17701 gttcattatc tgctgactcg accatagcct ccagtcgttt tatgattgcg actaattgtg
    17761 aatgtgtcat cggaggtcct ccttttctta tttctgttat attgtatcaa aagcatagcc
    17821 tacttgcatt gattttctct aaaaaaactc gttttttatg attttttttc tggtattttt
    17881 ccttcaggcc accaaagttt gatcaaagct tgggttccgt cattctcaaa acctttatct
    17941 gccaactctt catatagttt caatgcttgg gtagttgctg gcaatacaag atccattttt
    18001 tctgcttctt ctaatgcaat ctttaaatct ttgataaaat gtttgacaaa aaaaccaggt
    18061 gtgtaatctt cttttaaaat acgaggagaa taattgctta aagaccagtt agctgcactt
    18121 ccacctgaaa gcgtctcaat cactttcttt aaatcaagtc cgtttttttg tgcatagaca
    18181 agcatttccg tcattcctgt catcgtacct gcaatcatga tctgattagc catttttgta
    18241 tgctgacctt tcccagcaga accatgcaat gtaaatgtcg taccaaattc tttgaataaa
    18301 ggcagtactt gatcataaac ggcttcttct ccaccgacca tgatcgtcag tgtaccattc
    18361 tttgctccta agtcaccacc ggataccggt gcatctaacg catctgcgcc tttttctttt
    18421 gctgtttttg caattttttc tgccaggctt ggtgtacttg tagtcaaatc aactaaaata
    18481 tgtcctgaga tatctgcaga aaaaatcccc gactgtccat agtagatttc ttctacatct
    18541 ttggggtaac ctaccattgt aaagagaata ttactttgtt ctgctaccgc ttttggtgta
    18601 tctgcccaga tagctccttc tgttactaaa ggatcagctt ttgcttttgt ccgattatag
    18661 acagctactt cgtgtcctgc ttctagtaga tggcgtgcga cagcacttcc catgacgcct
    18721 gttccgataa aaccaagttt cattttttcg acctcctaaa aatcaaaaat ccttgcttct
    18781 atgctagtat acgctagaaa caaggatttt ttcataaaaa atgtttgaat tttatctttt
    18841 aaatgaattt aattttattt tgattcgttc aatcgagtct tcttcttctt ttcttcgcat
    18901 ctcttcttca tgataaacaa ccatgtcttg ataaaattcg atcatagctt cgccgaaatg
    18961 tgtagatgaa atatcataaa gtttagcttc taaaactttt ggatctttag gaacttcact
    19021 ggaaatataa tcaataagtg ctggtgcaaa gtcttcgtcg gtttcaaaag ttttccctaa
    19081 tgaaggatga tcgaacaatc ggttcaaata agcatttcct tccacgacac aaggtgttcc
    19141 ggctgccatc gcttctgtat aagtaagccc ttgtgtttct gaagtagaag cactgacaaa
    19201 ataatctgcc gctttataat aaagtgctac atcattgttg tccacttctc ctgtgaactg
    19261 cacaaattct tctaactgca gttctgagat caattcctct aactcttcta cataaggtcc
    19321 ttttcctacg ataactaatc tggcgttagg ataagcggag atgacctgag gaaacccatt
    19381 aatcaaggct tggatatttt tctcataaga aatcctgctt aaagaaagaa tcatcaaatg
    19441 gttttcctga agacctagtg actcccttaa tttgctgata tcctgctgag tgatatcaga
    19501 acgtttgaat ttttcaatgt cgataccagt aggaatcacg cgaagcggtg ctattacacc
    19561 atagctgcgt aatgtatcaa taacacgttc acttggacaa actacacctg tcgtatgatt
    19621 tgcaaataat ctagacaagt attttacatg ggattgcctg atcacttttc ctttaccgat
    19681 ataatgaagg tagtcttcat acatcgtatg gtaagtatgg atcagaggta ttttcaactt
    19741 cttagctacc atcttcccaa gtatccctgc tccgaattct gtatgggtat gaataagatc
    19801 tagttctagt tctttggcaa ccaaataagc ataccacatg cctctaacaa caactcttcg
    19861 atccttgaaa gaaataaatg gtacacttgg cattcgaatc acatcttttt ccaacttatc
    19921 cgcattagga tcggtcgttg taaaaatata gacttcatgc ccatgtcttt ctaattcttg
    19981 ttttaatgtt ttgatcgatg tagcaactcc gctgacttgc ggaaagtagg tatcggtaaa
    20041 aaagccgatt ttcatgtttt tcgcctccaa actagtcctt gacctgattt acttttttta
    20101 atttgctaga gactttttca ttcagtacac tttcgtatac atttttaagt tcttcaccga
    20161 ttttattgat acttcgctct tttgctgttt gataaccggc ttgtgtcgta ctcggtagtt
    20221 cttgattggt aatctgttgg atcagtcgga taaattcttc gtttgtcttc cccatatagc
    20281 agttcttgct atcttctaac cagccattat acactggaat atctcgaaca agtacgtttt
    20341 gtttgcttgc taaagcttct aaaacaacga tcccttctgt ctcttcataa gaagggaaaa
    20401 agaatagatc tgcacctgaa tacgcacctt ctatgatctc tccacgaatg taaccaggaa
    20461 acaggacatt ttcaggatga tcttctttta caattttccg gatgttccga ggaatcgagt
    20521 acatgggaac atgaccaaac caaataaatg tgtattcggg catcttctta gcaatttcta
    20581 cgaaatcaat gattccttta cgttcgaaaa acaaaccgac acaaatgatt actttttgat
    20641 ccggagacag tttgaaatat tctctgaact tttgttcttt cattggatca gggtgatatt
    20701 tctctaagtc gatcccattg gaaatagctt gaatcggcac acggatacca tagctttcca
    20761 acaactgttt tgaataaggt gttggcgtga tcagatagtc cgctttttgg tacaatccta
    20821 ccagatattt ttttacgata ggagaaagct ggttcgatcc gataaaggaa tttcggaaat
    20881 cttcctctgt agaatgcgca tgatagacaa ctttttttcc ttctcttctg gctttattga
    20941 ccatattatg actattgatt ccgtatgtat tgatgtgcag gatgtcataa tcttcttttt
    21001 catctagtgt ataagagata ccgacttccg tcagagcacg tttttgatga tcaagcgcac
    21061 gcccgattcc agattttgca agcatctttt cactttcaaa atataataat accttcacct
    21121 tacgtccccc ttcacttata tagtataaca tagcctacaa actgtcactc aaacttaagc
    21181 cctattttt
//
LOCUS       DAPCMT010000037        20957 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPCMT010000037 DAPCMT010000000
VERSION     DAPCMT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.037"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            591..1280
                     /locus_tag="RD743_001149"
     CDS             591..1280
                     /locus_tag="RD743_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0562976.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="RD743_001150"
     CDS             1267..2475
                     /locus_tag="RD743_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0562977.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="RD743_001151"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="RD743_001151"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDU0562978.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="RD743_001152"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="RD743_001152"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDU0562979.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="RD743_001153"
     CDS             complement(6578..7273)
                     /locus_tag="RD743_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDU0562980.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="RD743_001154"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="RD743_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDU0562981.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="RD743_001155"
     CDS             complement(8472..8663)
                     /locus_tag="RD743_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDU0562982.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="RD743_001156"
     CDS             complement(8684..9574)
                     /locus_tag="RD743_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDU0562983.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="RD743_001157"
     CDS             complement(9561..10328)
                     /locus_tag="RD743_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDU0562984.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="RD743_001158"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="RD743_001158"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDU0562985.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="RD743_001159"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="RD743_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDU0562986.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="RD743_001160"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="RD743_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDU0562987.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="RD743_001161"
     CDS             14899..15282
                     /locus_tag="RD743_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562988.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="RD743_001162"
     CDS             complement(15450..16208)
                     /locus_tag="RD743_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDU0562989.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="RD743_001163"
     CDS             complement(16226..17044)
                     /locus_tag="RD743_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDU0562990.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="RD743_001164"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="RD743_001164"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDU0562991.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="RD743_001165"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="RD743_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDU0562992.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="RD743_001166"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="RD743_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDU0562993.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="RD743_001167"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="RD743_001167"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDU0562994.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5991 a         4264 c         3837 g         6865 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac ctttttt
//
LOCUS       DAPCMT010000038        20436 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPCMT010000038 DAPCMT010000000
VERSION     DAPCMT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.038"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            38..340
                     /locus_tag="RD743_001168"
     CDS             38..340
                     /locus_tag="RD743_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562995.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            337..498
                     /locus_tag="RD743_001169"
     CDS             337..498
                     /locus_tag="RD743_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDU0562996.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            495..800
                     /locus_tag="RD743_001170"
     CDS             495..800
                     /locus_tag="RD743_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562997.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            800..1156
                     /locus_tag="RD743_001171"
     CDS             800..1156
                     /locus_tag="RD743_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDU0562998.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            1143..1361
                     /locus_tag="RD743_001172"
     CDS             1143..1361
                     /locus_tag="RD743_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0562999.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            1358..1777
                     /locus_tag="RD743_001173"
     CDS             1358..1777
                     /locus_tag="RD743_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDU0563000.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            1774..2331
                     /locus_tag="RD743_001174"
     CDS             1774..2331
                     /locus_tag="RD743_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDU0563001.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            2328..2624
                     /locus_tag="RD743_001175"
     CDS             2328..2624
                     /locus_tag="RD743_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563002.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            2701..3114
                     /locus_tag="RD743_001176"
     CDS             2701..3114
                     /locus_tag="RD743_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDU0563003.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            3460..3834
                     /locus_tag="RD743_001177"
     CDS             3460..3834
                     /locus_tag="RD743_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563004.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            3871..4038
                     /locus_tag="RD743_001178"
     CDS             3871..4038
                     /locus_tag="RD743_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563005.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            4064..4408
                     /locus_tag="RD743_001179"
     CDS             4064..4408
                     /locus_tag="RD743_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDU0563006.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            4413..4694
                     /locus_tag="RD743_001180"
     CDS             4413..4694
                     /locus_tag="RD743_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563007.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            4797..5111
                     /locus_tag="RD743_001181"
     CDS             4797..5111
                     /locus_tag="RD743_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDU0563008.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            5089..6783
                     /locus_tag="RD743_001182"
     CDS             5089..6783
                     /locus_tag="RD743_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDU0563009.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            6803..7981
                     /locus_tag="RD743_001183"
     CDS             6803..7981
                     /locus_tag="RD743_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDU0563010.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            7944..8630
                     /locus_tag="RD743_001184"
     CDS             7944..8630
                     /locus_tag="RD743_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDU0563011.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            8630..9790
                     /locus_tag="RD743_001185"
     CDS             8630..9790
                     /locus_tag="RD743_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDU0563012.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            9800..10675
                     /locus_tag="RD743_001186"
     CDS             9800..10675
                     /locus_tag="RD743_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563013.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            10672..10983
                     /locus_tag="RD743_001187"
     CDS             10672..10983
                     /locus_tag="RD743_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563014.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            10973..11326
                     /locus_tag="RD743_001188"
     CDS             10973..11326
                     /locus_tag="RD743_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDU0563015.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            11316..11717
                     /locus_tag="RD743_001189"
     CDS             11316..11717
                     /locus_tag="RD743_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563016.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            11710..12114
                     /locus_tag="RD743_001190"
     CDS             11710..12114
                     /locus_tag="RD743_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563017.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            12126..12734
                     /locus_tag="RD743_001191"
     CDS             12126..12734
                     /locus_tag="RD743_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDU0563018.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            12754..13116
                     /locus_tag="RD743_001192"
     CDS             12754..13116
                     /locus_tag="RD743_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563019.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            13119..13301
                     /locus_tag="RD743_001193"
     CDS             13119..13301
                     /locus_tag="RD743_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563020.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            13318..16737
                     /locus_tag="RD743_001194"
     CDS             13318..16737
                     /locus_tag="RD743_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDU0563021.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            16788..17525
                     /locus_tag="RD743_001195"
     CDS             16788..17525
                     /locus_tag="RD743_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDU0563022.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            17535..19826
                     /locus_tag="RD743_001196"
     CDS             17535..19826
                     /locus_tag="RD743_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDU0563023.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            19850..>20436
                     /locus_tag="RD743_001197"
     CDS             19850..>20436
                     /locus_tag="RD743_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563024.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7322 a         2988 c         4392 g         5734 t
ORIGIN      
        1 gatcaagtac tcaaattact gaaaggagaa tgtcgggatg gataagaaag cggcaatgca
       61 gcgaattatc gaattgactt attcagaaga ttggcaaaat gacaaagaag ctgcttcaga
      121 agtgatgagg cttggaagag cgatgtgggc agacaagagc aacaagccaa gaccacgaaa
      181 aatcgcaatt tggcacggtg acaaacttct agtgataggg acagctgaac agttagcaag
      241 tctcacaggc ttgcacgaga aaatcgtgag gaaaagagca aggtgtggct acacagacgc
      301 taagaagaga acgtttagat acgtggagga atcatcatga caacagaaga agtgattcaa
      361 atgcgtattc gaagccttca gcgtgagatt gacgatctgg aacgaacaaa ggcagtgatg
      421 gtcaatgaaa cggcgagaaa ggcaatcgat ttgcacatag agaatttaag aagggaaatc
      481 catcgattgg aggaatgagc gtggataaga aagcagcaat gaaaagaatt gctgaattaa
      541 ccaagtcaga atcttggcaa gaagacaaag aaatagttgc agaagtccaa aagctcggta
      601 aatcaatgtg gactgaaaag cccaaacgga gaacgccgag aaaaattgca atctggcatg
      661 atgaccgaat tttagtaaca ggtactgctg aacagttatc tgaaattact ggattaagca
      721 aaaacattat ctgggataga gctaggagct tatggattga ttcaaaagga cgacagttta
      781 ggtatgtgga ggagagataa tggatctcat tacacaatac agtgacatca tcctcaagaa
      841 aatcatgatg aagattcaga aagataaaaa atcaaaagaa cgagctgaat tagttaagtt
      901 agaaatggct gaaacaggag caggagtgcg aagtagcagg cattggaaag cagcagcaaa
      961 cattgaattt tattacaacg aaattcaaaa agggttcgat cagatgcgtg agctggatcg
     1021 gcaaacaaat tggagcaaga aacttcatca agatcgtttc aaatttgtag agaagtataa
     1081 agaaatatta gaagagtatt tgaggaggac agcaaatgat aaaaaaactc gttcaattca
     1141 gcatggattt atatgatatc gaatcaggag caacactatc tgtggaatcg gaccatctaa
     1201 tcataaattt tggtggaaag cgccagatta ttttgtgggt agttgatgat gtactgtttc
     1261 cagaaattgt tcatgatttc gaagaatcaa aagcggttga gtttgaaata gtgaaaaaag
     1321 taatggaatt gattgaaaaa tacgaggagg acagcgaatg ataccgaagt ttagagcgtg
     1381 ggataaacga aagaacgtaa tgagagatgt agccgtcttg cattttacta aaaacggcaa
     1441 aacaaacttt attgaatatt ggataaatcc taccgaattg aaatcatatc acgtgcgaaa
     1501 catcgacctc atgcaatcca caggaatgaa agataagaat ggtgtggata tatttgaagg
     1561 ggatatagta ttagtcagcg tgcgaaatgg cttcgattac ttagataata aagtctgtat
     1621 tgtcaaaaat tcaatagatt attccggatt agtttgtgcc actgttgatg aagacttaga
     1681 gtatcaaatt tttaacacag agctgtttga agaatacacg tacgaagtca ccggaaatat
     1741 atacgagaat agcgagttat tggaggaaca gcgatgaata aacaggaagc aatcaaaaag
     1801 ttagaaagta ttaaagcgat aggaaatgat gcaatagctg cttgctataa cgagagtata
     1861 aattcaggta ttacgttaat gaaaaaaata gacgaaccgc agaaaccagt tattccacaa
     1921 ctcgtggccg gttggcttga gaaatctacg gaccctttta caaaagctga aaaaatagcg
     1981 tatttaatca aatctaaaga tggtgattca tattatttct gtgattggtt tgtacgagat
     2041 ggcatagtga cgcaagagca aggagaagaa ttactcgctt gggcaacaag acaatcatat
     2101 gaaacactat tgagcctata caacggctac gaggttgaga aagagccgtt atgggcaata
     2161 aagaatgccg atggaaacta tcttactaaa tgtgctttat ggggaaaaga tggagtaaat
     2221 tatagttttg aatgcaatcc atctcatcga ttgcttttca ctgataaagc aacagcggat
     2281 gctgcagcat tgttggtgaa tggaacagtg gaagaggtgg tagaaagatg aaactaaaag
     2341 acggatttta cgctagcagt cacggtatcg gcggtttaat gctagatatg ccgacaaaga
     2401 accctaaaac acgcaagaaa tcaaaattca aagtcggtga catggtccgc tgtgaagcag
     2461 aagagttcgt ttatccgttc agaggatacg ttaaaaagat actgtcaaac tcagcaatca
     2521 ttcgtattga aaatacaatg aagtgtgatc gttcgactgc aaaacacaag cattatttag
     2581 ctgtagcgag attagttgac atggaagtaa tcaagagcaa ataaaaaaag ccggatcgct
     2641 ccgactgatt caataaatcc aacacattta ttatatcaca taaaggagcg gtttgacttg
     2701 atgcaattgt tacgagaggt agatttcaaa cagacaagat gtaatgcgag agatgtgctg
     2761 aagaactttc ggcgtttgga gcggatggca ggtcgctctt tgatagatat taagtcgccg
     2821 attattacgg atatgccgaa ggcaccaaag cacggcaata aggcagaaga cgcgatcatt
     2881 cagatgatgg atatagaagc ggagagagat gcgattctag cggctttgat ggctcttagt
     2941 ctaattagtc gccagatact ctactacagc ttctgtgtgc cagatagctt ctcaaactac
     3001 aggattagcc gtgaagtggg ttattcagaa agaagtatac aacggatgaa gtcggaagct
     3061 ctaatagagt ttgcagaagc atataaacac ggaagaataa ttgcttataa ataatttggc
     3121 ggttttttgg cggaatgatg gcggttttta gctatttacc agtgatatta tggtagtgtc
     3181 gaaagattag tgataggtct aagacaaaat aaaatgtaag ggaggaaatc tccctcatcg
     3241 tttaattaag cttcgataga cagcagcaaa taaactaaag gatgtggggg tcagctccta
     3301 cagttagttc atgtgttgct gtctattaat tgcaactctt tcggttttat tgagtattta
     3361 ctcaacttaa aaaaactgtt gtttgtagat gtaagctgtt cctatttgta catagtaaaa
     3421 aatgatataa taaaaatatt acgaaagggg tgagagtgca tggaagacaa aatgtattta
     3481 agacaaaaag ttttcacaaa aaaagatatt gaaaatcaaa ttaatggatt tatacagaaa
     3541 ttagaaaatg caaattctta tttgataaat aaggaactaa ataaagctta tgaccagtgg
     3601 aaagaagtat atgatgagtt gaaattgatt caaaatgaaa caaaattagt acgaacggag
     3661 aaaaagaatg aaaatagttt cttttttgat ggatatgcta tattgatgtt ggaaactgta
     3721 gcaaaacaaa atataaaagc tcccaaaaaa gaactatctg acaatattga taacgcatta
     3781 gccgagttaa ggtattacgt tatgcaaata aaagatgtta ggatcactcg ttgagtggtc
     3841 tttttatttt gcacaaagga ggtaacaaca atgtatagac cacaatactt agaacagaag
     3901 tatgaagtaa tcactgtgca aaatggtaac ggagagatag tacgaaagta tagaagacca
     3961 ataaagagcg atacatataa acgaaaggaa agcaatgaag ttattccatt gtatggcaaa
     4021 agaatagcta agcattaaat aagattgtga aaggagacgg gacatgaacg aggaattcta
     4081 tagatggcta ttacagttga taagagaaga tcgtttggtt aagttctatc agtctcctaa
     4141 atggcgcagg cttagagaga aagcgatgaa acgagatcac tatgaatgcc aagagtgtag
     4201 aagactaggt aagtatcata gagtagagaa cgttcatcat ataaaggaag tcaaggatag
     4261 acctgactta gctttagatt tagataatct tatttgttta tgtgttgaac atcataatga
     4321 agttcatggc agatatctta cagcattgga taaacaagag aagaagatag aaagctttgc
     4381 taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat caatgataat ggcagagagt
     4441 atgatacaga aaagattgaa gagtattcat cttatactca gggattaatt aaacgtttga
     4501 tatacgttcg ctatgtaggt attagggatc tgttatcaga taactgttgt agtaaataca
     4561 aagtgaatca agtaagagaa gcgttgaata aagataataa cgtcgaaaga ataaaaaatg
     4621 tttttggata tagtattgaa gagattaatt attacattga cttcgctgaa gctttcattc
     4681 cgatggtgag ataacccccc cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg
     4741 agggggctgt caggaaaaga gattttttcg aactttatca tgaaaggagg gctaaaatgt
     4801 ttaaaaacga attgtctcaa aatcgctaca gagaaaaatt acgccgctct ttaataagcc
     4861 aattggaaag tcagaaaaca aatattgagc cattcttaga taatgttgat cgttatatca
     4921 gtttatggga aacggcgata tcactggaag aagatatatc cgagaacggc attagactgg
     4981 agaatggtaa aaagaatgaa tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat
     5041 tgatgttgga taaacttgcc attactcctg aattggtagg tgaagcaaat gaatcaattc
     5101 ctgagttata agcatattga aaattggttc aaagctatag aagaaggcac tatcaaggta
     5161 tgcaaagagc aattattgct aaaaaattat ctagaagaaa gagtctttac tagagaagat
     5221 atttacttcg ataagcagat ggtagaggat tcaatcaata taccagcaca atactttcca
     5281 ttcgaattaa ttccgtggga aaaatttcta caatgtttta tttatggtgt tcgatggaaa
     5341 aaagataaaa cactagtgtt caatagatat ctttcattaa tgggacgtgg taatggtaaa
     5401 actggttttg cttcttggaa caacttcttt ctactaaccg ctaaacacgg tattaaaaat
     5461 tatgatattg atatctatgc caataatgaa agccaagcaa agactagttt tgatgatgta
     5521 tttaaagtaa ttaaagatca tcctgattta gataaaaaag tatttaaagc tacgaaggaa
     5581 gttattcaaa atatcgctac aaacagcaaa cttcgttata acacggcaaa tgctagaaca
     5641 aaagatggga agcgaccagg tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat
     5701 tcaatgataa atgtggctac ttctggtggt ggtaaaattc gagattatag agaattttat
     5761 gatacaacta atggtcatgt tcgtggtggt ccgcttgatg acattataga agaatcaaaa
     5821 atgattcttt ctggagaact tggaattgac aaggatggag cagaattttc tagtttgttt
     5881 ccatttattt gtcgcttgga taacgataat gaagttgatg atcccgacat gtgggaaaaa
     5941 gcttgtccaa ctattaatta caatgcaaat ttaaaacgga aaatgtttca agaatactct
     6001 caaatgcaac gtaatgctgg tttaagactt acgttcatga ccaaacgaat gaacagacct
     6061 atggaagata cacgatttgc tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa
     6121 gaatttcctg aaaaaatgga tgaagtgata ggaacagtcg attttgctga tagacgagat
     6181 tttgccagcg ttgggttgct aggaaaatac gataaagatg tttattttac acaacatact
     6241 tttatccacg aatcagccct tcgattacaa aacatcaaac gagaggttat agatatttct
     6301 atagatcaag gaaaatcaca gatcgttcat ggaaaaaata tagaagctga ttatattgta
     6361 ggttggtttc ttgaaatgag taataaatat tatattaaaa aaatcgctat ggatatgtac
     6421 cgtgcaaaaa tattgaagcc cgctttagaa gaagcaggtt ttactgtgga aattgttcga
     6481 agcggatctg ttacacatgg tatgttaaaa gatctggttg atgacctttt tattaatcaa
     6541 cgtttatttt ttggtgacga tgcgattatg cgttggtatt gcatgaatgt atatgaagag
     6601 catatttcta atggaaatat acgctatgaa aaaatagaac ctgaaactag aaaaacggat
     6661 ggcttttttt cattccttca tggtttgaat tttttagatg atatttatga ttctgctcct
     6721 gtaacagtca caaatagctc agtagaaaat acaggaactg gatttactcc tctagtattc
     6781 taacttgaaa ggaggtgaga aagtggggat ttttcaaaag gcggtaggat acttcacaaa
     6841 aaaagcaacg gttcctttag aagaatactt ttgtaaattg caagttgatt ttgtgtatcg
     6901 aaaatttgcg attgaaactt gtattgattt gattgcaaat gcgatgagta aagcggaatt
     6961 caagtcatat gaagatggaa aaaataaaaa gaatgatctt tactataggc tgaatgtagc
     7021 tcctaataag aaaaataatg caacagaatt tagaaaaaaa ctgatcagga gattaatatt
     7081 ctacaatgaa gtattgatcg tttctccgtc taataattct agcgaaatat ttattgcgga
     7141 tagttgggat gtcacagaat atgcattgaa agatgatgtg ttttctcaag tgcaaattaa
     7201 caacatagtc cttgatagag aatttctaga aagtgatgtt atctatataa aatacgcaga
     7261 tcaacaaatt aggcaactag tcgatgcgta ttatcaagcg tatgggaaac tcatttctag
     7321 tgccatgaat gtttacaagc gctctaacgc tcgtagatac gtactgaaag ggaatttatt
     7381 ccgaccgcaa gacaatacaa cacaagatca aatcaataaa atgatgacat cacaatttaa
     7441 ggcttttatg gaagctgata atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt
     7501 agaagatttc agcggaaact tccaaagcaa ttcaagagat ataaaaaact taatagacga
     7561 catctttgag atgacagcag cagcgtttca cgttccgaaa aacctactaa agggagacat
     7621 gagtgggtta tcggatcaag tggacgcttt tttaatgttc gaaatcatac cgattgctga
     7681 acttattcag gatgcgttta acgctagtct ctatgaagta gaagaatact tgtcagggaa
     7741 ttttgtacgt gtggatacaa ctatgatcaa gattactagc ttcaaagatt tggttgacgc
     7801 tattgatgta ggcattagaa atggagtatt tacaatcaac gaaggaagag aacgcgttgg
     7861 aaatgatcgc tctgataagg cgatggcaga tgaaatattt ataactaaaa acaatcaaca
     7921 agtatcgaaa ggaggtgagg cgaatgacga caatgaaaac atttctagca gtaaagaatg
     7981 aaggcgcagt accgcaaatt tttattcagg gatttattgg ttctagttgg ttctttgaag
     8041 ggaatactga caagggaatc aaaaatattt tggatagtct aggtgatcaa gaagaaattg
     8101 aagtagtaat taattcaaac ggtggagacg tatttcaagg gattgctatt gggaacttac
     8161 ttaagtcaaa taaagcaaaa gttaacgttg tgattaacgg cttagccgct agtgctgctt
     8221 caattatcgc aatggctggc gatactataa aaatctacaa caatgcacaa ttgatgattc
     8281 accgcgcttc cacatacgga gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac
     8341 tggaatcaat tgataaatcg gtaaaggctt catataaaac acgattcaat ggcacagatg
     8401 aagcattgca agaacttctt gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt
     8461 atggattggt cgatgaaatt atcgatgcag aaaatagctc aggtactgaa gctaaaaaag
     8521 aacaaagcgt tgaagaaatt ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg
     8581 catttacagc agcattaaat aaaacatttg gacaaggaga tgtaaaataa tgacagttaa
     8641 aaatttaaaa ggtgtaacag ctgcaagcga ccaattgatg aaagctttta aagatggtaa
     8701 cgaagaatct tttagcgcag ctatggtaag cttatctaag gaaattcagg ataaaatttt
     8761 agaagaagca acagcaaaaa atcaagatca attagtatta atgaaccgtg gtcagcgtgt
     8821 attaactaca caagaaacaa aattctataa cgaagtagtg aaaaacgaag gttttgcagg
     8881 ggtcgaagaa ttagtgccag ctactgtatt tgaacgtgta tttgaagatt tagaacaatc
     8941 tcatccacta ttgcaaaaaa ttacttttgt taacacaact ggtgtaacag aatggattgt
     9001 gtcacgtgga gtcaatccag catggtgggg taaactttgc gaagctgtta aaaaagtttt
     9061 agataatggc tttgatgtaa ttaacatgaa gcagttcaag ctatcaggtt atattcctgt
     9121 atgtaaggca atgcttgatt taggtccagt atggttagat cgttatgtcc gtactgtttt
     9181 agtagaatca ttgagaattg cattagaaca agcaattgtt gatggtactg gtaaagatat
     9241 gccagtcgga atgatgcgtg acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa
     9301 agcagaacct attacagctt tagatgctgt aactatgggc cgtttgatgg cgcgactatc
     9361 aaaattcaat atcgaaggtg tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt
     9421 ggtcctaatt gtgaatccaa ctgattactg gtctaaagta ttcccagcta agactgtact
     9481 aactgctaat ggagaatacg tacaagtatt gccagtacca gtttcagatt tgcagtcaac
     9541 ggctgtgcca gaaggaaaag cagttattgg ggtagcctca gattacttca tgggtgtagg
     9601 atctacacta aaaattgaag cttcagatga ataccatttt gttgaagacg aacgcattta
     9661 tctagctaaa caatatgcaa acggtcaacc taaacgtaac gatagtttca ttgtattaga
     9721 tattagcgct ttgggaacta ctactacaac tacaaaacca acaaccacaa caactacaac
     9781 acaagcgtag gtgatcagaa tgaagtatat tctttgtcag ccggcaatca atcggtttaa
     9841 atgggagctt gaagtttgtt taactaatct gaagaaacta ggaatcaaag atatcgtatt
     9901 gcttttcagc agacacgatg atcagattcc tatttttttt gagaaggaat atggtgttga
     9961 agttcatgtg tacgatgatc tgcgggacga caaagagtat attccttcga ttaaaccata
    10021 tttatggtgg aaatatttag aagaagatca ttcgcgtgag gacgaccgat atttctatat
    10081 cgattcggat gtcattttca ataaaagaat taatttgcgc aaattgcctt ctaaagatga
    10141 tgtttggtat tgtagcgact gctgtagtta tctaagtctt gattatatta gaagctgtga
    10201 aaacggagaa aatattctaa aagatatggc aaacattgta aatgttacag tagaatcttt
    10261 ggaaactata aacactaatt caggaggcgc acagtgggtt attaaccgtc ctaaagcgaa
    10321 ttattggaaa aaggtttatc tggattctaa tcggctatat cgctacctta gagggcaaaa
    10381 aacaaatata caaatctgga cagccgagat gtgggcacgg ctttggaaca tgatgtattt
    10441 caatattggt cctaaagttc acgaggaatt agacttttgt tttgctactg atccaataga
    10501 aaaagttaaa gaagtaaaaa tcttacacaa tgctggagta acaacaaacg atgaagattt
    10561 atttttcaaa gggagatacg tgacttctac gccttttgat gaagatttat catttgtaaa
    10621 caagaaaaaa tgctcttacg catatgttaa agcaattaag gcggtggtta gatgacgcct
    10681 gaacaagtga ctgaagaatt gctaatagct gtgaaggata atatttacgt tacctggaac
    10741 gaagaagatg agtcaattaa aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa
    10801 gtgagtacaa cactttcttt ttctcctgaa agcttagaat acggattgct aatcgaaaga
    10861 tgtagatacg actggaatcg tgctttagat gagtttgaac aaaatttcgc tagtgagtta
    10921 ttaggtttca ttcaacatta tgcgctacaa gaatatattg caggtgatgt gaatggcgaa
    10981 taatcgtaga ctcgaagaaa cattcaacga tggttggtta aagattttga cgcaaactac
    11041 caaaagaaat gaactaggaa aaaagattgg tgtagaagat acagaaatca cttctttaaa
    11101 atttagaaat ctttccatga gagatagtga tataacagct atggatgcga tgggatcgaa
    11161 attaactaag aaagtaaaga ctccatttca tccaatcgcc aagaaattta ataaagatca
    11221 atattttatc gtaatcgata gtatgcgtta caacgttatc tatgccgatt acgataattt
    11281 ttatatctat ttttatcttg aaagtgtggg tgaatatggt gattgataat tctaaagaaa
    11341 aagaacgttt aaataagcaa atttctgcta tcaaaacttc cttagaagag cattttggcc
    11401 tcaaactctt tcaagactcc gtaggcgagg atgagctacc tgatgatttt aattacttca
    11461 ttctcgaaac aggagaaata gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc
    11521 tatatctaac tttctattca gaaaatagag aagatttaac aggagactca ctagatatta
    11581 tttcattgat tcaaaatcgt tcgattcgtt ttcagagaat ggatcccaat catttaaaac
    11641 tagagaacca agatcgctat atcgatcaat tggtatttac gtttagacga ttattgaaga
    11701 gtgattgtca tggctaaaaa tagttgggag ctaaaaataa atggacatga tgaacttctt
    11761 gtgcggatgg aacgctattc aagcgagagc gaacgactga ttaacgaagc attgaaatca
    11821 aaaggttcgg ctattgcagt ggataggatt acagaaaaaa ttcctgtttc tgaagcagat
    11881 ttaagaagag ggcaccaaca cgcaaaaaat agtcgtccac ttaagactca atacattaat
    11941 ttgggtttca tcattagacc tacaagaaaa tttgagtatt taaaatatcc tgatttgggg
    12001 ataggtactt ctaaaagaaa tcagccggac gaatttatga gaagaggatt aggtcttgca
    12061 cttgatccaa ttacagaact tctgattcgt caattcgata aattaaataa atagggggaa
    12121 caacaatggc taaaacaaaa actgtagtaa caacgttcga taacgtaagt atcaaacgaa
    12181 ttgcttttaa ttttaagaac gcaggaaatg caatcgcaac gaattgtaat ggacaattag
    12241 atggcgaaac agaaatgcaa acggtggtta aaaaatgtgg agcgacagaa gtaaaatcaa
    12301 aatctaaacc aatcaatatg acggtaacaa ttactgcaca tgtaccgatg gaagtttatc
    12361 gacgtttcaa tgggttgaaa caagatggac gtattaaacc aggcatttac tcttacggtc
    12421 ctgattccgt aggcgaagat ttctcacttg ctgcagagat cgtggatgac ttcgaagaaa
    12481 atagaaagtt agttggtatg ttagcatgca cttcgaatac aggattaaca ttctctattg
    12541 aaaatggtgc ggatgaagta gctgcgttag aactagaaac aaaagttatg caagatgaat
    12601 ttggtaaatt ttatcatgaa gcaattgttg cagaacttga agaagactta acagatcaat
    12661 ggatgacgaa tctatctgct gatgtgatta aaaaaacttc agttgtgaca actacggcca
    12721 ctcaatcaca gtaaaaaaaa cggaggtagc gaaatgaacg aagattactc aaaaattgaa
    12781 ctaaacgatg gaacaatttt gaatttagaa cctaaactga atatcaagaa attattgatg
    12841 atcaatagag attttaacac agacgagttt gcaaaaatga ctgtgggaaa aggatccatg
    12901 gatatttctg ttattcaagg tgcaaaggct gtgtatattg cttaccgcca agcgaacatg
    12961 actgattata tttcattcga tgaatttatc gataaatggg attttgatat ggctactgcc
    13021 agctatattt atcaattgat gatgttcaaa caagcacgcg atgcttatca aaaagaattt
    13081 gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat gccaaagctc ttagttgaaa
    13141 cgtgggtcga tgtctattcg atgttgaccg acgttttttc tatgccttca gatttggttt
    13201 taagcgatat ctgtttagat gacattttgc aaatggctta caacaagagt gcttatgaag
    13261 gatggaaaaa ctatgcaata aaccaatctc agaaaaacta aagaaaggag gtaaaaaatg
    13321 gctaaaaaga gaacagaagc agaagtaact ttcatagcta acgatgacgg attgaaatct
    13381 acgttaaaag aaatcagtgc tgaattaact aaaaatagag cagaattaaa actagaacaa
    13441 gctcaattac aacagactgg ttctgaatca gacaagttag gaagtaaatt atcttcttta
    13501 gaaaagcagt atgaattaca aagccaaaaa gttgaagtaa ctagccaacg tttagccaat
    13561 gccaaaaaat attatggaga aaattccacc gaagttcaga aacttgagag agaactgatt
    13621 aaccaacaaa cagcgcaaca acgtttgtca aacgaaattg ataaaacgag taatacacta
    13681 gctcaagcaa aaggcgaaat acagacgtac gagtctacaa tgcaacagtt ggatagtgaa
    13741 caaaaaaatg ttcaagctag tgcttctctg attgaatccg aatacaaaaa atggcaagta
    13801 actgctggtc aatcagcttc tgaatccgag aaattagcga aagcccaaga atatgtttct
    13861 caacaatctg aaaatgcaga gaaaacgata gatatcctga gacgacagtt agaagctaca
    13921 cagtctgagt ttggcgctac atccacagaa gcaatgcaga tggaggcgaa gcttaatgat
    13981 gctgaacgtg aatttgaaga gttaggacaa gctgctaaaa atgtagatac aactaacttg
    14041 gacgatatcg gaagcaaaat agatatgaat aatttaatgg aagcttctga cgttttaagc
    14101 gacattggcg ataagcttac agaattaggg aaacaagcag tggactctgc taacagtgta
    14161 ggtagttccc agagtaaaat acaagctaat tttggtttga ctaaacaaga ggctgaagaa
    14221 ttaacgaatg tagccagaga catttattat aaaggttttg gagaatcgtt agatcagtcc
    14281 acagatgcat tgattttggt aaagcgtaat ttaggcgatt taaataatca agatttacaa
    14341 aatattacgg aacaagctat ggtcctagaa aacaccatgg gcgctgatat ggatgaaacg
    14401 ttacgtggtg taaatggctt aatggtcaac ttcggattga gcgctcaaga tgcaatggat
    14461 ttaatggttt cgggtactca aaacggttta gataaaacgc acgaattagg cgacaatatg
    14521 gcagaatata gccaattatg gagtcaaatg ggatattcag ctgatgaaac gttcggaatg
    14581 cttcaaaatg gtttagatgc gggtgcttat aaccttgata aagtcaatga cttagttaag
    14641 gaaatgggaa tatcgttaac agatggtcga tttgagcaaa acatggatat gtttagtgaa
    14701 agtactagaa aagcttttga agagtggaaa aatggtggag gaacacaaaa agacgttatt
    14761 aattccatga ttcaagattt tagcaatatg gatggtcaat acgaccaatt aaataaagct
    14821 tctacaattt ggtctgcact tggcgaagat aacgcgatga aagttgtcca atctttgact
    14881 gatgttaacc atacatttga tgatgttagt ggatctgcac aaaaaatgaa tgaagattct
    14941 actactccgt tacaagaatt aaacggaaaa atagctgaat taaaggattc attagctcct
    15001 ataggcaaca caatcataga tgcactcgaa ccagtaattg attttctagg aaagatggct
    15061 gatgcgttta ataatcttcc acaaccagta caggattatg ccgtagcgat tggcggattg
    15121 actgctgcat ttactttatt aatgccaata atagttggct tcatggctct aggtggtcct
    15181 actacattaa taataggagc agttattact gctattgctg gagttatagc aattataaaa
    15241 aactggggcg caattactga ctggtttaag ggaatatgga gtaaattcac tgattggttg
    15301 ggtggtactt gggaaagcat aaaagaaggt gcctcatcag tttgggatgg agtcaaagaa
    15361 acctggtctg gatttgtaga ttgggttcaa gaaatttggc aaggagtttc tgattggttt
    15421 ggagagttat ggagcggatt agttgaagga gcttccaaca tctggcaagg agtccaagag
    15481 acttggcaag cattcgttga ttgggtttca aatatttgga acggagtcaa agaagtatgg
    15541 tcgattattt gggcagacat tgtaggaatt gttcaaatac catggacatt aataacgtca
    15601 ttgattcaag ctggtattaa tattatcgtg ggtatctttg atgtagctgg acagttatta
    15661 ggcgcagctt ggcaagctgt ttggacacct atttctgatt tccttaaaaa tacttgggat
    15721 actatgacac aatggataag catcgcttgg aatggaattg taactacatt ccatactata
    15781 tttgatccag tagtggcatg gtggaatggt atatggacat ctattagtac tacggcttca
    15841 aatatttgga actcaattag tgcaacagct tctagtattt ggaatagtat caagaataca
    15901 atcactagct tggtacaagc agctgctaca gtaattcaaa atatttggtc aactgtatct
    15961 agttggttag gtggaatttg gaattcaatc agctctacag catcaaatat ctggagtagc
    16021 gtgactagta gtataagcaa tgctataaac gcagctaaaa gtgccattca aagtgtttgg
    16081 aatagtatat cttcgtggat cagcggaatt tggaacggta tcaaaaacac tgctttgaat
    16141 ctttggaatg gaattacaag cactattagc tctaaagtaa acgatggaaa aaatgcaatt
    16201 tcaagcggtt ggtccaatct aacaggtatt gtttccgaca tattcaataa tgttaaaagc
    16261 acgatagcta atatatggga aggtatcaaa aagactgtta gcgctccgat tgattggatt
    16321 agagataaaa tcagtagtgt ctttgataat ttgaatattt ctataccaca tattccgtta
    16381 ccacatttta aattgagcgg agaattcaat ccattgaagg ggaaaatccc aacattgggt
    16441 gttgattggt atgcgaaagg tagtgtgttt aattctccaa atattatcgg tgtaggtgaa
    16501 tcaggacctg aagcagtttt acctttgaaa agatctgtgc tgcaagaaat tggtgatcgt
    16561 atcttgagta gcacctcagt ttcatctagg acacaagcgg ttcaacctgt gaacaattac
    16621 gaattcaatt tcacaattga tggtaacgca gatgaagtca ctatgaagca aacaactcaa
    16681 caaatcattg atagcattac aaaagttcaa aatgataatg cttcggcatg gcgttaaaca
    16741 ggagagtatt tctcctgttt ttttagtatt aaaaaggatg tgaaaaaatg actgattgta
    16801 tacattctat aatcgatgga tttcctgatt atttgcataa attggcttta gctgaaagac
    16861 caaccatacc ttctccaaaa agacagagag ttgaaacttc tgttttagga aggttaggtg
    16921 gcttagtgca agattactcg tttgaagaca tgtcgtttac attgcactat aactatttag
    16981 aggatgtgga agaccatcaa gcgttcaagc aatcgtttta tatcatgcgt cattggttaa
    17041 actatgcaaa gaaattagaa ttctctgacg atcccaacgt ctattacgtt atccagacta
    17101 tcgatattgg ggatgcagaa aacgatattg ttgaatgggg agagttcgat gtaaatatta
    17161 ctgcgaaacc attcgcaaga gttcaagaag atgtacctat aaccgtagat aaaccacagt
    17221 catttaactt gctgaataat agtttagaag aaagttttcc aaagattatc atcactcctt
    17281 cagctacttc atgccagttc atcttaaatg attatgtgtt tagtttcgaa ggcttagtag
    17341 caggaactga tgtagtcatt gatagtgatt tgatgctttg ctacgaagag caatcggacg
    17401 gagatatttt agatcggtcc aacaaaatga agaccatgca atatccgaca ttgcaagtgg
    17461 atatcaatca ttttaattgc actggtttga gcaaaataca aatttatcgt aacgggttaa
    17521 ggtaggtgaa atagatgatc gataatttaa taactattta cgataaaaat gacgcgaata
    17581 atttagctga acatttatat gatacgcaag gtttaggcgc tttgtcagac tggttaacag
    17641 ctactgttag caataaacta aacggagccg agatatttca gggtacttat ccaataagcg
    17701 gaactaatgc agatttgatt gtagaaggac gtattattca gtgttatgta gatgaaaatc
    17761 gagcaaaaca gcgtctacgg atttattatg caaaaacttc tgtaataggc aatacgatag
    17821 aagtaaaagc tgaacctatt ttcaatgata taagaaaatc ggtgttgaat aaatatgaca
    17881 gcggaacaga aaaaatcact gctactcagg catggcaaaa cgcaaaagct ttagcgaaac
    17941 cagctatccc ttcgcagttt tctttctcgt cattagtaga tacgcttgct aatgtgaaga
    18001 tagaaaaagc gaatttttta gaattctttg gtggaaaaga gggatctatt ctagatcgat
    18061 ttcatgggga atttcttaaa gataataaca cattacatca tgaaaaaagg ctaggtacgg
    18121 atcataaaat caaagcgatt tatactaaaa atttaactgg tcttgactta gagatagatg
    18181 ctcaaagcgt tttagttgga gtttatccat tcattagcag ttcttcagaa ggagaagacg
    18241 agatcactct accagaagaa gtcattttca cggattacgt ggatgattat cctgctggat
    18301 atgtttcttt tgttgatttt aaagacaaag cgactgatgt agccacatta agggaagctg
    18361 ctaaagactg gttgaaaaca aacatagata aacaaaaacc acaagtgagt ggttcgattg
    18421 aattagtacc attgaggcat caaagaggct atgaaaaatt tgttgatcta gaaaaagttt
    18481 cgatgggtga cggagtagat gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa
    18541 ttgtggaata tacgtttaat gttctaacca attcatacga taaattagtt gtaggaaacg
    18601 tcaaaacaaa cttcttagaa aacacagaga ataatgtaag caatttgatt aatgatgcca
    18661 ttgatcaatt gaaaaatggt ggcgaaatca gtgatttaat caatgatatt gtagatcatc
    18721 aaactgatat gattactggt caaaatggtg gttatgtttt attagatcct aaagaagcgc
    18781 ctagtcgtat tttgattatg gacacaccag ataagaatac cgcaaggaat gttttacaaa
    18841 tcaacaatgc tggtattggt ttttctaaaa ctggcattaa tggaacatat gaaaccgcat
    18901 ggacattaga tggcggattc aatgcctcgt ttattacggc tggtgaaata gtaggaatta
    18961 ctattagagg tactacatta attagtgatg gcactgatta tagaacaagt attgctaatg
    19021 gcaaaatgac ttggtactca aaaaaagtta acaaagatat tatggagcta gaagcacgtg
    19081 attatgtaag tgctgatgcc ggtattgtat catacaccat gaaaactggt ggtggtttca
    19141 tgattagaaa tccacagggt aacttggttt ttagtacgtg ggataatggt aataacagac
    19201 cgtttctatc ttttggtgcg cccaatttca ggtatagcaa tgctagttat gtaacttctg
    19261 gcgacggtag ttctttaagc attaatggta gtgcgggtaa ctcatgggaa tttaaggtag
    19321 ctggtaggac tatgaaattt actagtgatg gtatgctaac gttgccaggt tgtttttttg
    19381 gttcatggga agatgggaaa cttgctaggt ttgaacaatc aacggtacaa gtatataaag
    19441 attttactgt tagaggtact aaaaactcaa ccgtaccaac agaacattat ggacaacgac
    19501 tattaaacgc ttatgaaact ccagaatatt atttcgctga ttatggggaa gccgttacag
    19561 gtgatgatgg taaagttcgt gttgatattg accccatgtt tgctgagaca gtaaatctaa
    19621 gtcgatatat gacacatgtg acacctacag aactagtttt gtgtgctgtt actcatgaag
    19681 atattgacca tttcatcatt gaaactagta agccaaacgt attagttaga tggaatttag
    19741 tggcacaccg tctagggtat gaagatatta gattaaaaga ggatacagca tatgatagca
    19801 cagtgcttga ccaaaaacgt ttttaaaacg aagacaagga ggtatataaa tggctagcag
    19861 tttatataat ttggctctag atttcagcaa agaattaaac tacaccaaag ctattatggc
    19921 tcgtcaaggt gataaaggga ttacagtgac tgttaaacca tttttaaatg gcttgcagat
    19981 ggatacgagt ggcggaacat ttactttaaa aggaacaacg ccatctaacc gttacgtaga
    20041 tagtgtggca actagtgtaa ctagtgaaga agtcacgttt tctcttgatg gcacatttat
    20101 gagtgaagca ggatattata aacactgcta cgtagaatat agaaaagaca atcaaatttt
    20161 aacaacgcaa gatatcattt ttttctcact aggagtgtct gacatttcgc aaggccaagc
    20221 cgatgaatac gtttcgcaat tggaagagtt gattcaaaag tataacgaaa cttttgatgc
    20281 ttttatggct gaaatcgaag gtagagtgga cagcttaaat aaacagatta ctgatttaac
    20341 tggtcaagct aaaacactac aagacaagtt agacgctctg aaagaagaaa tttctaaatt
    20401 aggtaacttg caggtgatgt acagtaacag catcga
//
LOCUS       DAPCMT010000039        20376 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPCMT010000039 DAPCMT010000000
VERSION     DAPCMT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.039"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            20..1099
                     /locus_tag="RD743_001198"
     CDS             20..1099
                     /locus_tag="RD743_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0563025.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1296..1997)
                     /locus_tag="RD743_001199"
                     /pseudo
     CDS             complement(1296..1997)
                     /locus_tag="RD743_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2011..2935)
                     /locus_tag="RD743_001200"
                     /pseudo
     CDS             complement(2011..2935)
                     /locus_tag="RD743_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3052..4131
                     /locus_tag="RD743_001201"
     CDS             3052..4131
                     /locus_tag="RD743_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDU0563026.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4163..4756
                     /locus_tag="RD743_001202"
     CDS             4163..4756
                     /locus_tag="RD743_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0563027.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4866..5093)
                     /locus_tag="RD743_001203"
     CDS             complement(4866..5093)
                     /locus_tag="RD743_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0563028.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5196..7784)
                     /locus_tag="RD743_001204"
     CDS             complement(5196..7784)
                     /locus_tag="RD743_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDU0563029.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7804..8463)
                     /locus_tag="RD743_001205"
     CDS             complement(7804..8463)
                     /locus_tag="RD743_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDU0563030.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8512..9015)
                     /gene="trmL"
                     /locus_tag="RD743_001206"
     CDS             complement(8512..9015)
                     /gene="trmL"
                     /locus_tag="RD743_001206"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDU0563031.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9236..10102)
                     /locus_tag="RD743_001207"
     CDS             complement(9236..10102)
                     /locus_tag="RD743_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDU0563032.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10211..10852
                     /locus_tag="RD743_001208"
     CDS             10211..10852
                     /locus_tag="RD743_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDU0563033.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10914..12275)
                     /gene="mgtE"
                     /locus_tag="RD743_001209"
     CDS             complement(10914..12275)
                     /gene="mgtE"
                     /locus_tag="RD743_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDU0563034.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12293..13192)
                     /locus_tag="RD743_001210"
     CDS             complement(12293..13192)
                     /locus_tag="RD743_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDU0563035.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13194..13991)
                     /locus_tag="RD743_001211"
     CDS             complement(13194..13991)
                     /locus_tag="RD743_001211"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDU0563036.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13969..14673)
                     /locus_tag="RD743_001212"
     CDS             complement(13969..14673)
                     /locus_tag="RD743_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDU0563037.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14804..15379
                     /locus_tag="RD743_001213"
     CDS             14804..15379
                     /locus_tag="RD743_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDU0563038.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15497..16174
                     /locus_tag="RD743_001214"
     CDS             15497..16174
                     /locus_tag="RD743_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDU0563039.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16253..18061)
                     /gene="pepF"
                     /locus_tag="RD743_001215"
     CDS             complement(16253..18061)
                     /gene="pepF"
                     /locus_tag="RD743_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDU0563040.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSGSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18237..19235)
                     /locus_tag="RD743_001216"
     CDS             complement(18237..19235)
                     /locus_tag="RD743_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDU0563041.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19461..20114)
                     /locus_tag="RD743_001217"
     CDS             complement(19461..20114)
                     /locus_tag="RD743_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDU0563042.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5890 a         4438 c         3635 g         6413 t
ORIGIN      
        1 tagacaagga tgaattcaca tgacgcaaac ccatgataac acagtttcac gcaagggaaa
       61 acatctttcc tactcagaac gttgccaaat cgctatcctg aaaaaggaag gctattccaa
      121 ccgtcaggtt gccaaagtgc tgggacgtgt tccgcaaacc ataaacaatg agattgatcg
      181 cggaaccatc acccagttaa aacgccaaac gcaaaatgga aaagtgtatg acacctacta
      241 ttctgtttat gacgctgatg caggacaggc cttctatgaa aagcaacgac tgaactgtgg
      301 cagacgcccc aaatgggcga acacaaatgc cttcattgat tgggcagatg acaaaatgtt
      361 aaatgaaaaa tggtcgccgg acgtcgtggt cggatttgcc agaaaacacg agctgttcga
      421 ttgtgccatc attcctggca caaccgctct ttatcaatgg attgaccgcg gaatcatgcg
      481 aacaaagaac atggacttgc ttgaaaaact gtcccgtaaa atcaaagcgg tccgcccagg
      541 acaccgtccc aacaagcgtg ttctcgggac gtcaattgaa gaacgcccta aagaagttga
      601 aacacgggaa acgttcggtc attgggaaat cgacacggta gtgggaaaca agaccaagtc
      661 ggatgccgtg ctgctgacgc tggctgaacg gcagacacgc tttgaagtca tcctaaagtt
      721 gaacgggaag gacaccgaat cggtggacca agcggttcaa cagctaagag aacgggctgg
      781 cgctaacttc gatcaattat ttaagaccgt tacatccgac aatggctcgg agttttctgg
      841 gctacacgat gctctgcaag atgtagctga cgtctatttc agccacccct atgcttcttg
      901 ggagcgtggg acgagcgaaa accagcacaa attaattcgc cgtttcgtcc ccaagggaag
      961 cccgattagt gaggtgagcg agaaccagat tttgcggatc cagcgttgga tgaacgatta
     1021 cccgcggaaa attttgggtt atgcgacacc acatgataca ttcgtccagg cattcaagca
     1081 agaacggttg gtggcttgac ccattattcc ttgataacga tcctcactcg ggacatggct
     1141 tcatgtctgg aaagctggcc tggggccaga atcgtctgaa gactatcata cctcagttca
     1201 ttatcaaggc ctcactgatt tagaataacg aggcagttac ggtatcaaac aaatgagtta
     1261 cctgtagtgg ctaacttaaa cttgaaattt acggacgtaa agcttgctgc caaattagcg
     1321 attgaaacta acgattgctt tctaggaatc aaggacaagc gaataccatt tgacgattga
     1381 tttgcttgct gatcctgtac attgcggacc ccccacatcg tcccgtagag aatcgccatt
     1441 ccaatcagag tgaagaaata aaaacttttg aaactcatat tacgacctgc atgaacttct
     1501 tgaaaagata cttcttgttc aaaaacagtc atgatgggcg cgagatcaac gttctccact
     1561 tctgccactt tactcgaaat tataccaata cgctgtaaaa attgatccat gacgtttttc
     1621 atcaacgtct gactcattcc tgcttgaccc acatggagcg aaacagatga accatcaatg
     1681 agataaaaac cagccaaatc atctgcattt agcttttcat tcgcttctac ttccgatagt
     1741 tcctgtactt caaataatgg tttatcattg acttcgactt gttctaagac tgtggtaaaa
     1801 ttttccgaaa gcgctgtttg atccgctttg attacaattc cagtattaat cgtttcgaga
     1861 ttgccacctt gatcaatctc gccaaaggcc gccataaaca tcaatcctaa aagaattgga
     1921 aaggccaatg tccaaaacaa catggattta tttcgcagca gtatttttgt gcgatattta
     1981 taaaaatgcc aaaacattat ttcccctcct ctagtctctc aaatccttgc cagtaatctc
     2041 taaaaagaca tcattcaagg acgcttcttt ggttgctatt tttccgtaac tgatcttttc
     2101 ttcttgtaag attgtaagaa tcggcaacaa tactgtattt agttcagtag ttgttacttg
     2161 gagctgacct tttttataag cgattccatc agaagctatt gttcgctgac aacacgtttt
     2221 ctgggagtaa tgggatgtcg atcgtcaaac gatccgccgt ccgtaccata tcttttagag
     2281 attctttggt tcctttagca atcacctgtc cttgatccat gatcacgatc tggtcgcaaa
     2341 gaatttctac ttcttccatg taatgtgttg tatagacaat ggttgctcct tgtcgattca
     2401 gctccttgat actttctaaa attttattcc gactttgggg gtctacagct actgtcggct
     2461 catctaaaaa aattaaccgc ggcttgtggg caatcccaca tgcgatgttc aaccgacgtt
     2521 tcaagccacc actaagttct cctggacgaa acgaaacata tttttctaat cccactaatt
     2581 gaatcacctc ttcaatcaag gctttccttt tctctttttc ggaaacatat aaaccgcaaa
     2641 aataatcgat attttcttgc acacttagtt cgtcaaagac cgcaacgtct tgaggaacga
     2701 ccccaatcat tgccttgaca gcataatttt caggagtcat tgttgtcccg aacaatttaa
     2761 tggttccttt gtcataattt aacaaagaaa gcatacagtt gatggcagtc gatttcccac
     2821 tgccgtttgg tcctaacaga cctaagactt ctccttcttg tacttgcaga tcaaaatgat
     2881 tcaatgctac aaaatctcca tagcgtttga ctaatttatc aatttcgata atcatcatga
     2941 aaaactcctt ccaaatttct tgttgatctg actataataa agtgcaagaa aagaaactag
     3001 tgacgattgg ctaaaccaca catgacattt gtcacaatga aggagtctgc tatgcataaa
     3061 tctcggctac tcaacgaaac tagctttcta ctggttatct cattgctttt gctggtaagt
     3121 caaggattcg ttgctatgca agttatctat ttattgatcg ccgttttatt cactggtgtc
     3181 agttatttac tgcctacaaa gccaaattgg attttccaag ttattctatt tcttttatgt
     3241 gcctttttac ctgatcttgt cctgtttctg ccagcgacga tgcgagtgat cgcccctgaa
     3301 gaaagccacc ttgattactt gtttttgtta ggttttttgt tgatttcgac ggttggtaat
     3361 catcccccca tcaaggtcgc ctttgtcgta attttgctgt ttttttcctt ttacttacga
     3421 aaaaaagatc aacgaacgac cgatcttgag aaaaagtaca ttgtctccaa agatactcat
     3481 tgggaaaaac aacaagagtt atcggaacaa aataaagcat tggccctgtc acaagaaaca
     3541 caattagcct tgcaaattgt ggaagaacgg aaccgaattg cccgagacat ccatgacaat
     3601 gtcggtcatc tgctgtcaag tgcgatttta caaattggtg cgcttgaaat gatcaatcaa
     3661 gaaccaaaac taaaagaacc attagaaaat ttaagtgcca ccgttcacac tgggatggat
     3721 cggattcgtc aaagtgtcca tgacctccat gagacatcga tctcttttca acactcctta
     3781 gagtttctga tcgctgattt tccttcttct gttacgattg aaggaaactt gtttgaagga
     3841 ttgaatgaaa cgcagcaaaa ctgcttctta atggtcatca aagaagcctt aaccaacagt
     3901 atcaaacata gtcatgccaa aaccgttgtt ttacattttc gaacgttgcc ggccttttat
     3961 cgcttgcaaa tcacgaatga tggcaccagc ccacaaaaaa attctgctcc cggtattggt
     4021 ttgaaaacga tgcgtcaacg tgttcaagaa caaaagggac aagtccatat tttccaagaa
     4081 aaccagcaat ttcagttaac gatcatttta ccaagggagg gacaccaatg ataacagtag
     4141 tcattattga gatgatccat ttgtgacgaa atcgttacaa accattttag aaagcacgaa
     4201 agagatccgt gtgttaggca ttggtcattg tgccaaggat gcgctagtct tatacgaaac
     4261 ccatcgtccc gatgttttgc tgacggacat ccgcatgcct aaacaaacgg gcatcgatgc
     4321 tgccaaagaa attttggcta aatttcccaa agccatcctt ttgcttttga cgacattcaa
     4381 agatgaagaa tacatccgcg aagcattttc catcggtgtc aaagggtatt taatcaaaca
     4441 gaacttgcaa gcgattattc cttctgtcaa atccgcttat aatggacagg ccgtatttgg
     4501 taatgaaatc atcgagactt tcactcaaat gatgaagaat aaaccgacga ttgatagtca
     4561 tgcttatgct tcttttagtg aacgtgagct tgcaatcatc aaagaagtag ctgccgggaa
     4621 aaacaataaa gaaattgcag atgccttgta cttgagtgac ggcactgttc gcaattacat
     4681 cagccaatta ctagaaaagc tagatttacg ggatcgcacg caattggcta tctattttta
     4741 tcagcatttg caataagtct tatctaacac aaaaaaacct gaatgatgct ccttcgcatc
     4801 attcaggttt ttttcacatc ttctattcaa ttgtcccgca aaccatttcc tcacgattga
     4861 aagaatcaag gattcgcagg gctgccagga ttcgttggat tgttcggcgt gttaggcgga
     4921 aaaataggcg ccttgtctaa cgttttttct agttttgatt cttcacgatc gtctcgttct
     4981 acatcaggac tttcattcaa tttgaattct ttttccatct catctaactt tttaaagtca
     5041 ttgccagatt cttttgctaa tttcgctaat ttatcttcgg tttctttagc cataaagtaa
     5101 cgcccccctt ttgatttcag cttagcatat cacactgaaa aaacaaaaga aaacgctaac
     5161 caacgatcat ttttagctat ttctcatctt taaaatcaag cattttttgc cggtataaaa
     5221 tcgtagggag aactgttttc cataccaatc atcggatcta ctaactggtt atcgatcact
     5281 tctgctgtaa gaacgaaatc tttagaaaca gcaggttcta cctcttcttt cttttcttct
     5341 ccaacaaatg aagaaaacag atctggttcc actgtttcca ttacttggtt tttcgtttca
     5401 ttcgtcccac tgtcatcttc tacaggtagc ttttctttta caacccgtgg ctcttttgta
     5461 tagttcccat cttcaaatgg atcatttccg accaaagaat cttcataagc tgctagtttg
     5521 gtccggagag tgatattcat tttatcatta tccatgatcc ttgatttcaa cgtagtcatc
     5581 cggcttgctg attcattcgc tacacaagtg acataggctt gctcttcccc taacaaaatc
     5641 aacagttcct tactgcgagt cactgccgtg tagagcaaat tacgctgaag catccgctga
     5701 tattgatgga ccatgggcaa aataaccatc ttgaattcac tgccttgtga tttatggatt
     5761 gagcaacaat aagaaagagt gattttattc cattcgtttc ttttataagt gacctcattt
     5821 gcatcaaact ggataactaa ttcatccact ttatcttcag aatcttttgc caagatgata
     5881 cccgtgattt ctcccatatc tccattgaat acattcaatt cgggagtatt gactaattgg
     5941 aggactttgt ctcctatccg ataaacggtg tcgttccaat gtacttcctt ttttccaggt
     6001 ttccctggat tgaatatctc ctgcatcatt ttatttaacg catcgatccc tgcaggtccc
     6061 cgatacattg gtgctaatac ttggatatct tgtgcacgga atcccttatt tttcgctttt
     6121 tccacaattt tagagatcaa gggttcaatt tgataagcat cacttcggaa ataggaacga
     6181 tctttttgat tctttgtgaa atcttttggc agacgtcctt ctttgatctc atgggcaaga
     6241 ggaataatac tagaaccatc accttgccga taaatctgat tgagttccat tttgggaatt
     6301 tgttcgattt ccaaaagatc atggagtact tgaccaggac cgacagaggg aagctgatct
     6361 ttgtctccta ctaagataac ttgcatattc gtcggaatcg ctttgaataa tgtgttagcc
     6421 agccatgtat cgaccataga catctcatca acaatcaaaa gtccgccttc taattctttc
     6481 gtactgactg aaggtgtttt ttcccgtcca ttcagcccca gaagacgatg gatcgtactg
     6541 gcaggcagac ctgtcgtttc gttcattcgt ttagctgctc ggccagttgg tgctgcaagt
     6601 agaataggaa aaatttcttg ggtatatttt gaaggatcca attccagtcc gttcaactct
     6661 gcaaaaagat tgacgattcc gttgatcaca gtagttttcc ccgtacctgg tcctcctgtt
     6721 aaaataaata gaggggaacg gattgcttca gcgatagctt gttcttgcga aggtccatac
     6781 tgtatgccga atattttttc gatacgccga atattcttct gcacttcttt ttcaggataa
     6841 tggatttctt ttttacgact gagaaggcgc tggatcgaat taccgatccc ccattcagaa
     6901 aagtacaaac tgttttcgta aagattagtc tcttcttgtt gtatctttcc ttcttcaaca
     6961 agttggataa ttacagtagc aacctgatca ggctggattt caattgggcg acttgtttct
     7021 aaaatacgga tcgtttgctg aagcaatgtc tccgccggta cgtatgtatc tcctgtttga
     7081 actgcatgct gaaaaatctg atgcaagata gctgctcgaa ttcttctcgg tgaatctgct
     7141 gcaataccga tttgttcggc tagattatct gcacgtttga acccgattcc ttctatgtct
     7201 tcgactagct ggtacggatt ttcttcgatg atatccaaag actcgttctt ataggcctgg
     7261 tagatactga aagctaactg actaccgaac ccgtaacgat tcagtcccat gatcacttga
     7321 tccatcccgt ggtttagccg aatcgtctca gcaagcattt ctctttttgg cttcgtcaaa
     7381 ccggagattt gttttaatac cgaaggatca gcaatgattt tttcgatagc atcttctccc
     7441 agcaattcta caattttttc tgctgttttc ttcccgattc ctgggaattt ttcgctggaa
     7501 agatacgcta cgatcccatt tgcagttgtt ggcttttctt gctcatatct ttcgagttta
     7561 agctgccttc cgtatttagg atgatccact aattctccat aaaaacgata agtctcattt
     7621 tcttgaatat cgccgaaact tccggtgact acaatctctt tttcacggta atcagctgtg
     7681 gtttcactga tgtgtaccaa aagcactttg taaaaattac taggattttg aaaaaaaatc
     7741 gcggcaactg taccaacgaa ataaggcatt tcttctggct gcatcggaag ccgcctcctt
     7801 cttctatagt aaaggttctg gtgctgtttc agttcctaaa aaatctgttt ggttccatac
     7861 tttcagttca tatggcaata atctgcaagg ctcttcagtc tctaaaatcg tcaaactact
     7921 attgaacaat ccacccattt cacgcaactc acttagctct tttcctgtca tccattgaat
     7981 ggctgcagtt aaagaagctc catgtcctac gaataaaagt gggccatctt tatgttgggc
     8041 aaccgcatct gctacaacag tttcgatacg tgtgattgcc gattcaatcg tttctccgtg
     8101 aaaaggagaa ggatcataca gatctaaatg attacgcaaa ttcggcagat tatccggata
     8161 tttctggtac atttcatcga tcgactgacc ttctaaagta cccagcccta gttctcttag
     8221 cttatctgta taaaaaatct ctaccggacg agcaagctct ttgttgatac cttctgctgt
     8281 atcacgtgct cgctttgctg tactggaata aattgcttta aaaggaacat attttacctt
     8341 tttcccaaat gctttgattt cttcgtaact ttgtggcaaa agcggagaat cgccattgct
     8401 cccttgaaaa cgtctttcct gattccattg tgtcttccca tgtcgggtaa aatatagttc
     8461 catacgcagc aaactcctgt ctttctttta ttttactcta taataaactg tctaatccag
     8521 cttgtcattt tcatagtgat gcgtcaattc taaattaggg aatccttgct ggcgaagtgc
     8581 ttcatacact atcattgcag ctgtattcga taaattcaat gagcggacat gctcatcatt
     8641 catagggatg cgtaaacact tttcttcgtt ttcacgcata aatgtttctg gcaaaccggt
     8701 tgtttctttt ccaaataaaa agaagtgctc ttctccatca gcaaaatttt cgtcactata
     8761 tactttatgg gcaaattttg agatcaaatg tagtttacga ttacctaaaa atgttaaaaa
     8821 agcttctaaa ttactgtggt aagtaatatt tacatcattc caataatcca accctgcccg
     8881 ttttagatgt ttgtcatcag ttgaaaaacc tagtggctcg attaaatgaa gcggagtatt
     8941 cgtggctgca cacgtacggg caatatttcc agtgtttgca gggattaatg gttcaaataa
     9001 aacgatatga ttcatcagga tcattcctct catatttatg ttcgtttcta ttttctgaca
     9061 atttgatttt tgcataaaaa aaaacgtatc aactcggtgt cagtcactgc gagtcgttac
     9121 gttagtgaag ctcttctcac taacttttat cagaacagga acctgataaa aaccaatgaa
     9181 aaaacaactt gttgctaata taacaccctc aacttttttt tgcaacctct actcattaaa
     9241 aactgctaga aaaacgtttt ctcttagttc cttttaataa ttctatgtca attgtaatct
     9301 tcttcaaagg attcttatat aagtctgctt tgattcgctc ttctacctgc cattctaaag
     9361 gtgacatggc caaaagatct ttttgatttt cctctggtat ctcaaattca taggttacac
     9421 gctgacgttc agttaatttc attgcttcag caaacttggc tactacacgt tcattggaat
     9481 aactctgttt ttcaggttga ttcggataaa aagcctgacg tagttccttt aaataatttt
     9541 cttgtggcac caccttcaac acttctccat cttctgagag tacacgttgg aattcttggt
     9601 aatgagaagg cgaaaagata tttaaaaccg cgttgaatgt ttgatcagca aacggcaaat
     9661 tggtcaaatc agctacacac caaaatgctt cggcagattg ttctgtagct gcgtagactc
     9721 cttcttttga aatatcaaat ccaacagcat tttgaatctt tttcaatttt gaaagttcat
     9781 tcaagaagct accttctccg cagccaacat ctaatagcga gacttcttca ggtaaatgct
     9841 gcgcaatagt ctcaataacg ggctgataca tgccagattg gatcattcta cgccttggaa
     9901 cgaacattcc ttgatcgtat tctgttttaa ccgcatggga taaaaaataa agtgttcctt
     9961 ttttggaaag gtcaaaacgg tgattatttt tgcatatcaa gccattatca gactttttta
    10021 cttcttcttg gcaaaccgga cagcggaaca gctgatgatt ttctgccaaa aactggcgtc
    10081 ctcggtcaat tttttttaac atagttcccc ctcatttctg ttttatctta ccgttagatg
    10141 ctcactttgg caactgctaa aaatatgata caatgcctag aagaaagtga aagataaagg
    10201 agaagattag atgatcaaag aattttgcgc tgaaaactac acatctattc ctctagcaat
    10261 tgctagagga gccgatcgga tcgaattgtg tgacaactta gcggtcggtg gcacaacccc
    10321 gagtactggt gtgatcgaag aaacgttaag ttatgctggg gaaaaagata tccccgtaat
    10381 gacgattatt cgtcctagag ggggcgactt cgtctataac gatatcgaat tgaaaatcat
    10441 gcatactgat ttgattgaag caaagaaatt agggacggat ggcgtagtat ttggttgtct
    10501 tactccttca ggctggctag acgaagaggc cttagaacta ttgatcgaaa cagcagaagg
    10561 gttgcagatc accttccata tggcttttga cagtattcct gaagatcggc aattcgaagc
    10621 aatcgattgg ctggtagaac atggtgttga ccgtatcttg actcatggcg gtcctgctga
    10681 caaaactatt gtagaaaatt tcccacggct taaagaattg attgcctata cagatggccg
    10741 cttaatcatt ttgcctggtg gtggtgtcac tgcagagaat gcagcacatg tagctaaaga
    10801 attgaacgta agcgaagtac acggaaccaa aatcgtggat cttcagttat aatcttataa
    10861 acaaaaaaac atttgcaaca ggcataatgc ttattgcaaa tgtttttgtt tttttacata
    10921 aaatagccca taaataaaga agcaatattg aaataaatca gtacactcgt caaatcgctc
    10981 aaagtcgtga taaagggacc gcttgctact gctggatcaa atcctagacg atccattagc
    11041 atcgggatta aactacctgc aagattcgcc actgtgattg caaatagcat agccatccct
    11101 atgacaaacc ccaagataaa attatgtttc caaaaaccta cgattagaaa aatcgtgatc
    11161 cccgtaacag ctcctgtgac aacgccggtc aagacttcag ccaaaacaag tcgaacaaat
    11221 ccatgatctt tctcatcaga aatcgcaagt cgacgaacag ccacagctag cgactgggtt
    11281 ccagcattac cggcagttcc tgtaatcagc gaaatgaaga ctgctaaaat actcgcttca
    11341 cttaccaagt cttcatagtg actgattaaa gaagcggtcg acatacccaa aaacaacaga
    11401 gtgatcagcc aaggcaaacg tcgagacgct gcctttactg gattttcatt aatttcttcc
    11461 acattgaccc cggcaagtcc tgaataatcg ctagcagctt catcatcaat aacgtcgatg
    11521 atatcatcga ctgtgacgat tcctaacaaa tgatcatcat aatcggtaac aggaagagct
    11581 aagaagtcat aatctctaaa tgtttgtgcc acatcttctt ggtcgtcgcc tacatgaact
    11641 gaaagcactc gttcgctcat cagatcagaa atcattgcgt cgtcatcatt cacgattagg
    11701 tcgcgcagag aaataacccc tactagctga ccttcttgat taacgacata gatgtaatag
    11761 attgtttcag ctacatctgc ttcgttcttc agcacgtaca tcgcagagcg aacagtttga
    11821 ttggctacga tcgaaacaaa ttcagttgtc atgatcgcgc cagccgtttc atcctcgtaa
    11881 tggagaagtt ccttgatttc actggcatcg tctgccgaca tcaaacttaa atatttagca
    11941 atttgctttt tatccagcgt attaagcaag tcaacagcat tatctgtata catctcagaa
    12001 agcatatcag ctgcatagct aggacgcatt tcatctaggt attctttcat atgttcgttg
    12061 tcttcttcaa tcacatcgaa catatccgct aattctttag gcgacagata ggaatagatc
    12121 agctgcctgt tctcttctgt aagagattgg tagaattgcc cctgctcata gatatgcatc
    12181 tctaaaaaac tgtttctgaa tgcctgaata ttattttccc ttaaatcttg agtcaactgg
    12241 gcaaatcttt cctctaattc ttgattttca tccaaaccaa tctcacctcg ccttattctt
    12301 caaaccgtct cacaactgcc tccatgtctg cggctaaagg ctgcttgaac tccagcaact
    12361 cttctgtaaa tggatggtaa aaacgcagat cataacaatg aagggcttgt ctttttattc
    12421 ccagatccat tcgcccgcca tacatatcat caccgagcag ggcacagcca attgcggaaa
    12481 aatgaactct tatttgatgt gtccttcctg tatgcagctg gatatcaact aacgcgattt
    12541 ctgttagtcg cttctctatc cagtactcag tctgtgccga acgtccttca tcagagacca
    12601 ttcgcttcaa tagcgaagaa taatctcggg cgatcggcaa atcgatatgt ccatgtgccg
    12661 ataaatctga aagccttcca ctgactaagg cctgatattt tttcacaaac ttcttttcac
    12721 gaagttgcat atctaatttt gcgtgcgcaa atccatgctt tgcaaaaagc atcaaaccag
    12781 atgtatcccg atctagtcgc gtcaccacat ggattacttg gttttcgtaa ccttgttttt
    12841 tataataagc ctttacacga ttagccattg tcccatttgg atggtattgg gcaggaatag
    12901 acgaaacgcc tgctggttta tttactacca gaacgtgttc atcttcatac acgatatcga
    12961 tcggtgtttc atccaaaagt acggtttcat gttctccttc agcaggaatc acaatggtta
    13021 ccttatcatc cttagctaga gaaaacaaga cattttctac ttgatcatta actttgattt
    13081 gaccgccttg aaattttatt ttcgctaaca gaccctttga gatccctttt tcttttaaaa
    13141 aatattttac ttgctgagaa tgatctttct cataatccca tgtaaactgc atatcaatcc
    13201 tcactaataa aggcatcttt cacacgatgc caaaaatgca tatgtcgata agaagcaaaa
    13261 tggatccgtt cttctgctat tcgataataa attgagcgga tatttgcttt tgcgacattc
    13321 aattgatcaa ccgttacaag ataatcttct gtattttcca attttatttc tagccattca
    13381 tcatgggcaa tcacgatcgg agaacccaaa gtacgaaaca ctcgattatt cagtgaagca
    13441 atctctgcca actgaaaggc attgatacta ggatggatca cagcaccgcc gacactttta
    13501 ttatatgctg tcgaaccagt aggcgtagaa atcgtcaatc catctcctct aaaactttca
    13561 aacagctcgt ttttgatgta tacatcggct accatcgtac gattggcccg tttaatcgtt
    13621 gattcattca gtgctaaaaa atgctggtcc ggtgtttcat ccaaataact gatccgaaca
    13681 tccagcaagg gataactaac actttgttcc cggttcgttc gtaagctgtc tactagttct
    13741 tccagctcgt aatcccgcca atccgtgtaa aaccctaaat gacctgtatg cacgcccaag
    13801 aaacgcacat cattcagaca atgactgtag tgatggaacg ccgacaataa tgtgccgtct
    13861 ccgccgactg agatgaccaa ttctggctca tgatcatctc gttgaatccc tgcttgttct
    13921 aaaagtgcgt tcaaacgttc tgtcacttct atagtctttg cttctttatt atgaacgatc
    13981 gctactttca tcccctgcct cctctctttc gctttctttc ttcaaaagcg ttcgatagta
    14041 gacatcatct gattttcctc ttccatggga gaataaccgc tgtgcttctt ggatttcttc
    14101 acggatactg gacatctctt catccaactg ataggcagct tctgccgcac gctgaagtcg
    14161 ttcactcatt tcttctggaa aaacgccttg atatttataa ttcagcgaat gctcaatcgt
    14221 tgcccagaaa ttcatcgcaa gagtccgaat ctggatttcc gccaaaattt tcttctcacc
    14281 gctgatcagc tgtacaggat actcgatcac cagatgataa gaacggtagc cacttgcttt
    14341 tttatttgtg atgtaatctc gttcttggag aactttcatg tcttttcttt tacggatgat
    14401 ctcaacaact tgatggatat cttctacaaa ctgacacatg atccttaacc cggcaatatc
    14461 agacatttct tcttctaaac gtcccattgg aatatgccgt aattgtgatt ttgttacaat
    14521 actatcgact ggcttgactc tcccagtgac aaattcaatc gggacatgtc gattttgttc
    14581 acggaactgt ttgcggatcc cccgcagttt cactttcaat tctgatacag tttgttcata
    14641 tggtgctaaa aatgcttccc aatctcgttc catagctgat acctctactt cattaacatt
    14701 cattgtttat tctaccatat atgcaaagtt ttttcgtact cttgatttat gcattgacca
    14761 aaaaaagaaa attaggcaaa atagacaaaa aggagtcgat caaatgacac aaatgcttga
    14821 aatcgaatat aaatctatgc tgacaaagca agagtacgag atattcatcg ctcactatca
    14881 attaacagat aaagatttcc gtgtacagac taatatttat ttcgataccg ttgatgaaca
    14941 gctgaagaaa caaaattgtg gcctacggat ccgtttcgtt ggtcatcaag ctgaatatac
    15001 attaaaaaca cctgctgaaa aagggcgtct cgaaacaacg gataccttta taacagaaga
    15061 agcagaagca tttatcttcg aaaaacgtct gcctcgttca ggcgcagttt ttcaaaaact
    15121 ttctgatttg aatatcgatc ctgcttcatt aatccaaacc ggaaagctta caacaaaacg
    15181 ggctgaattc cctattgaag aaggactttt agctattgat gaaagctggg gagacaatct
    15241 acacgatttt gaactggaac tagaggtagg ggatgcatct gtgggaaaaa ttgcttttat
    15301 aaatttttta aaacgttttt ctttacctta tcgtcctgct aagaacaaga tccaacgtat
    15361 gcttgaagct aaaaattaga aacctttcat tttcacaagg cacttgagtt cattttttta
    15421 cttttttctg ttaagttata ggatgtgaaa gaatctagtc actagttgta tagaatctat
    15481 tttgggggag attcatatga tcgaaatcta tttatttgtt aatcctttag gggaatactg
    15541 tttcgaaatg gaacaacagt tattacaatt tatcgaagaa gagtacggga caccatcaaa
    15601 agaaaagatg caatttcgct ttcttccttt ggtcaatttg cagacaattg gggatgtcat
    15661 gcaacaaaaa ggaatttctc agaacaatct tgaggaacgt aatcacttat tctcaatgac
    15721 ttattctgca gcattggact gcaaagcggc gcagtttcaa ggaaagaaaa aaggccgccg
    15781 ttttttgatc aaattgcaag aagcagtcgg ctgtaatgac ataccatatt ctcaagaact
    15841 tgctgaatcc atatttgaag aaatcggcgg cgatttgaat atgtttaaag aagatcgtca
    15901 gtctgacttc gttaaagaag tattcttttc tgatcaaaaa attgctcggg aaatgggcat
    15961 cacaaaacac ccttctgcag tcgtttacaa ttatgcctgc gaccgtgatt ttggtgtttt
    16021 agtagaagta aaaggtacaa gtagcctttg gagaatcaca gagctgtgta aaacaacaga
    16081 taactcatat caagttttta atggtgaaca tttacataca aatgattctc agttccattc
    16141 aagaactagc catttacact tgctttctaa ttaaaaaaat acgtcgactc ccttacaaga
    16201 gagccgacgt atttttttcg tttctattcg agaaaatcaa aaatagaaag ccttataaac
    16261 gatcgatcaa ttcttctaat tcatttagac gttgttcaaa catcgacatt gcatcttcaa
    16321 tatatgcagc ctgtgtcata tcaactcctg cttttttcat tacttccaca ggataatcgc
    16381 tgttacctgc ttttaggtaa gccaagtagt tctctagtgc ctctggttcc tggtttagta
    16441 ttttcttcgc cagtgctgaa gcagcagaaa agccagtcga gtattggaaa acatagtaat
    16501 tataataaaa atgcggtatc cgtgaccatt cgaatttaat ttctgggtct tcttcgactg
    16561 ctggaccata atattttgca ttcaatttac cataactacc acttaggtat tcactagtca
    16621 atggcacacc tttttcatct tccgtatgca tgaaatgctc gaattcagcg aattgtgtct
    16681 ggcggaaaac tgttccttta aacccatcca agtaatggtt gagtacataa gcccgtacac
    16741 gagggtcttt ttctgtttcc aataaatact ccgttaggat attttcattc gttgtcgaag
    16801 caatttctgc taaaaagatg gagtagtcgc cgtacacata aggctggttc gaacgagtga
    16861 aataactatg aacactatgt cccatttcgt ggacaagcgt aaatagctga tccagcgtat
    16921 catgccaatt caataaaata tatggatttg tgtcatagct tcccgaagaa taagcaccgc
    16981 ttcgtttccc tttattttcg acaacatcga tccaacgttc agagaatgct ttttctacga
    17041 tggccatgta ttcttcaccc attggtttca gtgcttctaa agctttttct tttgcttctt
    17101 cgtacgtaaa ggtaattgga gcttccccta agaccggtgt ataaagatca tacatgtgca
    17161 gtttttccac ttctaataaa cgtttccgta attccatgta tcgatgtaac aaaggcaaat
    17221 gcttgtttac cacgtctacc aaagtatcgt atacactttc aggaatatga ttattgctca
    17281 aagacgcttc tctggcagag ctgtaattac ggactttcgc tttaaaatta tgtcctttta
    17341 tatgtgtgcc taaagtagaa gcaaatgtat ttctaaattg ttcgtaaaca ctgtacaatc
    17401 ccttaaatgc tgcttcgcgc acccttcgat ccgtgctttc tagcaactga ccatacacgc
    17461 catgagataa ttggactatt tcaccatttt ctccttcaat cgttggaaaa actagatctg
    17521 cattattcaa aacagcaaat gtatcacttg aagcatcaaa gatttcacct gctccagcaa
    17581 gaagagattc ctgctcagca gataagacat gggctcgatt atctactatt tgctggatat
    17641 aatggcgata gacttccaat tttggttctt ctttgaaata ttgccaaatc tggtcatctg
    17701 acaattgcaa tatttctggt tcaaaccagg aaacggcttc actaactttc gaaaacagac
    17761 tgcttgctct tgcataaagc gcttggtaat ctgtatttcc agtgtcttga tcgtttttaa
    17821 ggtgcgcata tacataaatg acttcagttt ggcgataaac cctcaatacg aattcaatcg
    17881 catttaaaaa ttgagaagcg ccttgatcaa gtgttccttt gtgtttttca gattgtttta
    17941 actcttctga taattccaaa tatttctcat caaactcttg gtcgcttgaa aagatcttgg
    18001 tcaagtccca agttaaattt tcaggcaatt cttctcgttt tggcaactgc tttacttcca
    18061 tgaaatccac tccttttctt cccttagctc tatctatcaa aatagatcat agtctaacat
    18121 ccacatgtca cagcatctaa cttttctact cattcatgtg tctgcttcca tcaattttag
    18181 cataactaaa tcttgtttta catagaaaac cgattcctag cctattttta tacttactat
    18241 tttttcaaag aagacaagtg tatgcattcg ttaaaaaact gctgatagac catctcttca
    18301 tcaatcatag gaaaaagcca ttccctttta tgttctttta tttggcaaag ccatgagttg
    18361 aaatcaggaa ctttctggtt ttccaaggct tcttcgtaca acattcgata aagaaaaacc
    18421 tgttcttgta aataaaagaa aaaccgactt ttttgataga tccacgatga tagatgcaaa
    18481 agatgtttat gacgcaagta gcattgttct tgtatggata ctgtattctt ttgcttgtga
    18541 ataagttggc gtgtcagcca tttcttccca tcttcactca ctttttctat ttttttcatt
    18601 tggttcattt taatttcgtt actagtgaat atttctttaa ggtccctact agaaaaagtc
    18661 cagcagctac tgcaaaagga aatatgccct gatacagtct ctgtaatatg atgatacaaa
    18721 taaagctttt gtttcttgag atcagctttc cagcaatgca ctcctctttt tctattatag
    18781 tagcagcaac ttttttctat caatgagaat tgcgattgtc caagaaaaga attaccaagt
    18841 atccaccata cttggtaact gttattcaaa taattttggg ttctttcaat aaatctctgg
    18901 tgagacagtc tagaacattg gatttctatt gctagattcg ccaacaaaat atccggcctt
    18961 tgccgcaacc tcggcaaata ggcttccaaa aatactggtt ctactgactg tcccagccaa
    19021 tccatgaagc attcctttaa ctgtaagtgc tcttttgatt ctctttcact aaagaccgaa
    19081 cagttatctg atttttgatg ggcaaaatga gcacattttt tctttccctt tttcaaaatc
    19141 actctttttc gacagccacg acaataatat ttctttcctt tttctgcatg tttagcaaaa
    19201 cagcacttat tatattcatt cattgcaagc agcatttttc tccctctgaa cttatttacg
    19261 tctaagaatt tatttacacg aaaaaagagg gcgtgaaaaa gctattctgc atcaagtaat
    19321 aagaacagct aaacaaaaat agttcccaat atttttcgtt tggttgggct tgttaccgca
    19381 gatgcaagct tttgaacaca tttatacgaa aaaaagactg cgacacaatt tttgtcacag
    19441 tctcttacta taatgctgat ttatacaaaa taaaatttcg ttcgctcgat agcattacgc
    19501 gccatcagac attgtccata ttcattcaat gtttctggtg tacgatcgct cttttcagca
    19561 aactccaaaa ttcgagcaat ctgattctct acatccgctt ggttgagatt ttccatccag
    19621 aagtgaacga ctaggtaata gcgctcttcg aataaataca aatcagtcca agcagactct
    19681 agttcgacat ctgttgcaag ttggatcact tgttcaaaat cttcaaaagc aaaagttgct
    19741 tgtctttcct gttcttctag ggcgcttcca gataattctt cctccggatc gctgccaata
    19801 atctgctgac gaagaaagtc actcacatct tctgaatcaa cagtagaatc ttctgattga
    19861 gaaatttctt catccggaag atttttactg ataaacagtt ctaatccgtc tccttttggc
    19921 aaaacttgga aagtgacagc ttcgcttcct cggaactcct cttcgatatc aacttcttcc
    19981 aaaatactat aaaagaaatt ttcgatttct ttgtgattgc caagtaaatc caaaaatgaa
    20041 acgccccgtt cctctaaatc ttcatgacca attagtacac ggatcgtatt ttcattaata
    20101 tgttccattt ccattttgct acacctcact ttgccacaat gtttaatata gattcattgt
    20161 agcgaaatca gcttaaatgt aaagaaaaaa aatctcgctg ttaagcgaat ttaataaaac
    20221 cagcggattt ttcaaccttt caagctatct tatttaacta taacattttt ggcagacgaa
    20281 tactatatag ccgacttttc agctaatttt ccctctctat ttttcgtgca atcatcatct
    20341 tctactacct ttttatacat ctaatcaaga atactt
//
LOCUS       DAPCMT010000040        19728 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPCMT010000040 DAPCMT010000000
VERSION     DAPCMT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.040"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            102..749
                     /locus_tag="RD743_001218"
     CDS             102..749
                     /locus_tag="RD743_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDU0563043.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            833..1777
                     /locus_tag="RD743_001219"
     CDS             833..1777
                     /locus_tag="RD743_001219"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDU0563044.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1912..3363
                     /locus_tag="RD743_001220"
     CDS             1912..3363
                     /locus_tag="RD743_001220"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDU0563045.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3384..4220
                     /locus_tag="RD743_001221"
     CDS             3384..4220
                     /locus_tag="RD743_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDU0563046.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4220..4879
                     /locus_tag="RD743_001222"
     CDS             4220..4879
                     /locus_tag="RD743_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDU0563047.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            5005..5694
                     /locus_tag="RD743_001223"
     CDS             5005..5694
                     /locus_tag="RD743_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563048.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5700..6656
                     /locus_tag="RD743_001224"
     CDS             5700..6656
                     /locus_tag="RD743_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDU0563049.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6700..7212
                     /locus_tag="RD743_001225"
     CDS             6700..7212
                     /locus_tag="RD743_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0563050.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7468..8016)
                     /locus_tag="RD743_001226"
     CDS             complement(7468..8016)
                     /locus_tag="RD743_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0563051.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8105..9286)
                     /locus_tag="RD743_001227"
     CDS             complement(8105..9286)
                     /locus_tag="RD743_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDU0563052.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9522..11030)
                     /locus_tag="RD743_001228"
     CDS             complement(9522..11030)
                     /locus_tag="RD743_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDU0563053.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11189..11380
                     /locus_tag="RD743_001229"
     CDS             11189..11380
                     /locus_tag="RD743_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563054.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11583..12500
                     /locus_tag="RD743_001230"
     CDS             11583..12500
                     /locus_tag="RD743_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDU0563055.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12493..13566
                     /locus_tag="RD743_001231"
     CDS             12493..13566
                     /locus_tag="RD743_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563056.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13714..14232
                     /locus_tag="RD743_001232"
     CDS             13714..14232
                     /locus_tag="RD743_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563057.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14213..14605
                     /locus_tag="RD743_001233"
     CDS             14213..14605
                     /locus_tag="RD743_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDU0563058.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14681..15616
                     /locus_tag="RD743_001234"
     CDS             14681..15616
                     /locus_tag="RD743_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDU0563059.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15749..16528
                     /locus_tag="RD743_001235"
     CDS             15749..16528
                     /locus_tag="RD743_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDU0563060.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16521..16838
                     /locus_tag="RD743_001236"
     CDS             16521..16838
                     /locus_tag="RD743_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDU0563061.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17082..18692
                     /locus_tag="RD743_001237"
     CDS             17082..18692
                     /locus_tag="RD743_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDU0563062.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18849..19697
                     /locus_tag="RD743_001238"
     CDS             18849..19697
                     /locus_tag="RD743_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDU0563063.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6377 a         3521 c         3965 g         5865 t
ORIGIN      
        1 ataataaaag tttggttctt taatttttaa acatgacaga aaattttttc ttcgttatac
       61 ttgtaataga atactatttt ataacaaagg ggatcgaatc aatgaaaaaa actgttattt
      121 atactagttt atttgcagct ttgtttttag ctgcttgtca atcaaatacc aatcaaggca
      181 catctgggaa tacgtcttcc agtagcgaac agcgctcttc acaggtatcg gactcctcaa
      241 caaaagcaag cacatcttcg tctagccaga caaaaagttc tagtgaagca agtactcagt
      301 cagatatcaa agtgtctgca gctcgtgcga tcgaattgtt ccaagataaa tacccagata
      361 ctgcaattac tagtttggaa ttagatagtg attggggatc ttacttctac aagatagaag
      421 gtgtcgatga tcaaaaagaa tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga
      481 cagagaatcc agaaactcta gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg
      541 gtctggatgt tgataaattg atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag
      601 taggaagcgg tacggcaaca gattgggatt tggataaaga tttaggtact acttattggg
      661 aagtcaaagt aaaagacggc aatcaaagta catcagtcaa gatcaatagc caaacaggcg
      721 aagtactgtc aactgaacaa gatgattaag aatttcttcg gaaaacaaga gttgattttt
      781 cttttagtca tgatacacta gacaaaataa cttgtttgaa ggagcgattt acttgagttt
      841 agaaacgacc tttgccaatc atatctttgc caatcctttg atgaatgcat ctggtgtcca
      901 ctgcatgact acccaggaat tagatgaact ggctcattca gaagcaggag catttatcac
      961 taagagctgt acgatcaatg aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc
     1021 tttagggagt atcaattcta tgggacttcc aaacttaggt ttttcttatt atctagagta
     1081 tgctcttgcg tatgaaaaag tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc
     1141 cgggatgagt gtccaggaaa acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa
     1201 tgggatcact gaattaaatt tatcttgtcc caatgtgcct ggaaaaccac aactagctta
     1261 cgattttgag gccacttatg aaacactgaa agaagtattc agcatctttt ctaagccgct
     1321 aggaatcaaa ttgcctcctt attttgattt tgctcatttt gatcagatgg ctgatatctt
     1381 gaatcagttt ccattgacct atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat
     1441 cgatacagaa caagaagcag tagtcatcaa accaaaagaa gggttcggcg gcattggcgg
     1501 agagtatatc aagccgactg ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc
     1561 agaaatccaa atcattggta caggtgggat tcggacagga caagatgcat ttgaacatct
     1621 gctatgtggc gcaagcatgt tacagattgg taccgagctt cataaagaag ggccggaaat
     1681 tttttctaga atcataaaag aattaacgca gattatgtca gaaaaaggat atacgtcaat
     1741 tgacgaattt aaaggcaaat tacgcaccat ttcatagcaa agagacgaag gtcatgattg
     1801 atcttcgtct ttttttataa aaaacactcg tctgttcatt cctgtgcaag cgtactcatt
     1861 gtatgttaga cgaacttgtg ggaaaataac agaggcaaaa aggaggataa tatgaatgat
     1921 gcaacagaat tagcaaaaga gcttaaagaa aaagaattta gttttcaaga actagttacg
     1981 gatatacaga aaaaaataaa gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa
     2041 ccattcgaga gtaattccca cgagctattg attccccagt cagaaaaagg gccattggct
     2101 ggtatcccat ttcctttaaa aatgctcggt caagaaaaaa agggatggct agctacttca
     2161 ggatctcgtt tatttgaaac acaccgagct tctcatacat ctaattatgt gcggcaagca
     2221 gaagcgatag gtctagttcc ttttggacaa accaatgcgc cagagtttgg tttcaaaaat
     2281 attacagacc cagtcatcta tggcccagca agaaaccctt ggaaccttga atgtacaccg
     2341 ggcggatcaa gtggaggagc tgctgctgca gtttcatcag gaatcgttcc tttagctggt
     2401 gctagtgatg gcggtggttc tattagaata cccgcttcgt tttgcggatt gattgggctg
     2461 aaaccttcgc gagggacgat gccagttggt ccatatgctt ggagaggatg gcaaggagct
     2521 gctattgatt tcggcttgac agtttctatg agagacacgg aggctttatt ttatgggatg
     2581 agaagtatcc attccggtgc gccatatcaa gtatcgccag ttgagtggca aacacatcca
     2641 agaaaaaaac ggttgaagat agctttttgt gttgattcac cgatccactc acagattagt
     2701 gaagaggcag tacttgctgt aaaaaaagct gtagatttcc ttggaaaaga agggcatgat
     2761 ttgacagaga ttccttaccc actagatggt cgtaaactga tcacgtctta ttatcagatg
     2821 aatggcgcag aaacagcagc aatgatccgc tcgattgaaa caggcatcca gcgagctgtc
     2881 agaaaagaag aacttgaacc catttcttgg actttgttcc aatatggtca gaagctctct
     2941 gccgctactt acatcgattc tctgcatgta tgggatgaag cagcaattgt catggaaaac
     3001 ctacatcaag aatacgatct attcctttca ccaacgactg catttaccgc ccctaaaatc
     3061 agcgaagatt tgcaaagtga tgcgataaga gcacagatgg aacacatcag tgattgttct
     3121 gaagtagaag gtttagacgt tgtttcttcc atgtttgagg agagcttgaa gatcacacct
     3181 tatacacagt tggctaatct gactggacaa ccagcaatca gcttgcctac tcatttgaca
     3241 aaagaaaaca tgcctctagg aatccaattt atggctgcca gaggaagaga agatttgcta
     3301 tttcaaatcg gctatctatt tgaagcaaat catcagttcc atttaccaaa tccatggaaa
     3361 tagcagaaac aaggagaaga agaatgaaga aaaaaagatt gtttgctcta ataactagtg
     3421 ccttttttat tttaagcgct tgcggtacag ctgccgctaa tcaggaagat aacagctgga
     3481 gtaatgtaga aaaaaagaaa gaattagtag tagcgacttc tggcacactg tttcccgcat
     3541 cttattataa tgatcaaaat gaattagttg gttatgatgt agatttggca aaagaggtgg
     3601 ctaaacggtt aaatgtcaaa atctccttta aagaatacaa cgtggatgga caaatcactt
     3661 ctgtttcaaa aagagaatca gattttgctg cgaacgattt tggtatcaca aaagaaagag
     3721 aaaaaaaatt ctccctttca gagactttca aatattcatt tgacagtatg atcgtacgaa
     3781 aaaaagatga ttcaggaatc cactcacttg aagatcttaa agggaaaaaa gcagctggcg
     3841 aacccaatac tagctatatg cgtttagcgg aaaaacacgg agctaaatta gtcacatatg
     3901 acaatgccac gaatgatcag tatttaagcg atgtagcaaa tggtcgaaca gatgttattc
     3961 taaatgatta ttatctgcag aaaatggccg tatctgcttt accagatatc cctgtaaaaa
     4021 tactggagaa cgtttatttc aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg
     4081 atgctctgaa aaagaaaatc gatggcgtat taaaagagat gaaagaagat gggacgacaa
     4141 agaaaatatc tgaaaaatat tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg
     4201 ctgagtcggt gagtgagtga tgtactttcc tctaattgat ttttccctca tgtggacttc
     4261 acttcctttc gtactgcaag gcttggtata cacattaggg attggcttcg tgtcgtttct
     4321 tttaggaaat ctggtagggt tgcttttgac agttttaggt cttttggatt ggctgcctct
     4381 gaatgtattt attcgttttt atttatcgtt ctttagaggc attcccgcac tagtcctgct
     4441 gtttctgtta tattttgggt taccttatca attaagtgcc ttaacggctt cagtcatctg
     4501 ctttacgatt accagcagtg catttattgg tgaaatctat cgaggatcac tagcaggcgt
     4561 ttcttcaggt cagtgggacg ctggtttagc ttctggtttt tctttctttg gggtattgaa
     4621 atatatcgtt ttgccgcaag cttttcgaat ctcagtaccg gcactcagta acgtagctat
     4681 ggatctagtg aaaggtactt cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa
     4741 agcaaagatc ataggcggtc ggacattcga ttatttttct ctctatattt tagttgcact
     4801 gatttattgg gtcatttgta tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc
     4861 tatgcatcag tcaatctgaa aaatagaaat ggtgctagcg tggctggcac catttctatt
     4921 ttttgtttct tgtatattct gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg
     4981 aaaatatgtt cggaggtaga aaacatgaag accccgctaa ccatcgaaat agaatcctcg
     5041 ttataccaat attgcatcga gcaaggcggc ttggttgtag aagaagtcac gatgcctgat
     5101 gaccaaggaa ttgtagatac gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg
     5161 cgatgctatg agctgaaagt gacaaaagca gattttcgct ctacagccaa actgtcattt
     5221 gttggacatt ataattattt tgttttacca agtaaactat atgaagaagt aaaaactgaa
     5281 atccctacag gaataggtgt tttagtgtat cgtccgtaca cggttgaaga ggagatgcct
     5341 gcctctggta catttctgat tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa
     5401 gaagctttga caaaccgctt catggcttct ttattcaggg aagtaagaaa agcaaagcaa
     5461 atggagcgag gcgtaaaata ttttccaaca gatcaattgt acaaagaatt gaaaagacgt
     5521 tcagaaaatc aagatccatt ttctaacgaa cgatattatg aacggttttt agaggaaata
     5581 gctagtcagg caatcaatga gttgcaagaa gaagttatcg cgcttcagca tgattatgaa
     5641 tatttgaggc aacaacagca gatccgtcgg ttgcctacag agccattgga gtgaaaaata
     5701 tgtactatcc ttatttaaga ggaaaacaat ttgacctatt agcattaaaa gaagcactgt
     5761 cgcgaggatt gctctccaat aaaatccaac cagtgatcga accagtacgt gattccgcta
     5821 cgcttaaaaa tgtgatcgaa ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa
     5881 atccccaagt aggacaattc aaattattcg atcagcatgt gcacagttgg acagtcaaag
     5941 aaaatagctc tgtcgtccct gcacagatct taacgccaga aaatctcagt gaagtactgg
     6001 attcacctcc agcgtttctt gttttcgatg gacagcacta cccaaaagat acagaggtgt
     6061 ggaaacagtt ggcaggagtt gattcgaaat ttttgatacc agatactagt cgctttcgca
     6121 tctggctgcc cgaaaacaaa attgtgatcc gtgactcttt tcaaacgaga aagcatgttg
     6181 aaagttatgc agataaaaca gatgactttt ttagtgatga ctatcttttt ttccacgcag
     6241 acggatatat tggattttct gatttcacga tcgaaggtag ccgttatttc gataaagggt
     6301 tccctagccg agcgcttgct atccatctaa cgtatattga tgcttacggc aatatacgcg
     6361 tgaaacattt tgtctctgac agtaacgact ctgctaaaga ccaagcattg aagtttcttg
     6421 aagcaggaga taaaatgaaa aaatggatca tgcgtcacca tcatcaactc ttgattactt
     6481 caggaatgat tgaattattg gctctttacc agcaacaaaa attccctgga ctgggagtgt
     6541 tgaaaaagtg gtcacttatg catcatttgg aacttattag tcacctgcta gatcatccaa
     6601 aagattggct taaatattat tcaaaagttc aggaactata tgaagtgatc caatgattag
     6661 ataatgtaaa tgaaaaagga atagaaaaga ggaaactgga tgtatattcg gcaaacaagg
     6721 aaagaagaag tagaagaagt ggtagaaatc attaatgccg cagcggattt cattgcttcg
     6781 caaggctctc ctcaatggca aaacgggcag aaaccaacaa aagaaacgat tttgaacgat
     6841 attgaaagaa aagaaagcta tgtattaata gtggaagatc aaattgctgg aacagctgca
     6901 ttagtctcaa gcgtggatcc cgtttacaca aaaatagatg gtagttggaa agaatcaact
     6961 ggtccttatt tatcgattca tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta
     7021 gcaaaaccgt tcttagaaca tttgttgacg attgcagcca gcaaaaatat ccaagatatc
     7081 cgtatcgata cttatccagc taatgaaccg atggaaaaga ccatctattc tgctggattc
     7141 acttatcaag ggatgattga atttcctttt gaacacggcg aaagaaaagc atatcaaata
     7201 atcatcaatt gactataaaa tcatctgagc aaagataaag acattaacac tactgaaaaa
     7261 aacaatcata tgatagccaa gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa
     7321 gaatttccga aaatgcttct caaatttttg tggaatttta gcgtatttta cgaaatagct
     7381 gctttctgaa actcccttca tcgagaggtc gtgacatagc ttttgtcacg acctctcggc
     7441 ttatcctacg agtattattc gatacattta tatcaataat ccttttttta ttttatctgc
     7501 agcttcttgg aacgctccat cgtttgacac gactaaaagt gaggggatag ctttcaaacg
     7561 tttcaactgt gctaaatctt ctacataaac cgccagtctg gctgaaatct gttcagatga
     7621 atctccacgt tttttcaggc gcgattgcaa tgtttcttta gagatcgtaa taaagatagg
     7681 acggactacc gtcccgaaat actcatataa atgccaaaaa ccatttgctg tcaaaacggc
     7741 gtaacaattt cctactgtca gtttcttttt aatttctgct ttactggaac catatttatg
     7801 ttgagcatat tggtcccact ctgcaaactc atttcccttg atcatctgat tgaatgtatt
     7861 gtcagaaaca aaataataat cctcttgatc tttttcccca ggtcttggtg atcgattcgt
     7921 gtaagtaatg atcttttgtt ccggctgaaa aatagcttct gcgagtgaag tttttccaga
     7981 accagaagga ccgataaata catagcaata attcattgtc atttctcctt tgatgattga
     8041 acaagtgaaa aagctttgga aaatatttcc aaagctttta aaagtaactt tgcttttttg
     8101 tatattatac cagcaagcca gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta
     8161 gcaaaatttt caatccgtgt tttgcgacaa gatttccttt ttcttcattt aatcccttca
     8221 tcgcaccaga aatgatcccg atagaagaga agttggcaaa tgaaacgagg aagacagata
     8281 taattgctgt tgtacgctca gtaaaatgga aattaccatt tgctaattct gtcatagcaa
     8341 caaattcatt tgtaaccagt ttagtcgcca tgatactacc ggcatccact gcttctttcc
     8401 atgggattcc gctgatgaag gcaagagggg caaagatgta acctaggatc tcttggaaag
     8461 tgatgccgaa tatcccgtgg aagatagcat tgatcatggc aatcaaagca acaaaaccga
     8521 taagcatagc agcaaccgtg atcgccacat ggaaaccatc aagaatgtat tctcccaaca
     8581 tttggaaaaa tgtctgttct ttgttttctt caatgatcag ctcatcttct ttttcttcta
     8641 atacataggg atttacaatt gaagccagaa tataaccgcc aaataagttc aaaacaaggg
     8701 cagtgatcac atatttagaa tcgatcagtg ccatataaga tccgacgatc gacatagaaa
     8761 ctgtagacat agccgataca ctcatggtga acaaacgttt ctcagatagg aagggtaatt
     8821 ccttttttat ggaaataaaa acttccgatt gtcctaaaat agcagaagct actccattat
     8881 aagattccaa tttgcccatc ccgttgattt tacttaatcc tagaccaaca gctttcataa
     8941 ataatggaag aatcttgata tagcgtagaa taccgataat agcagaaata aatacaattg
     9001 gcatcaatac ggaaagaaag aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa
     9061 agtcaacacc ttgcccagca aaagcaagaa gatgatcaaa catttttgcc aatacagaaa
     9121 caaccgctgt accaaaagtt gtacgtaata atacgaaacc aagaacaagc tgtaaaacga
     9181 acatcacaat tagtggtttc aatttgattg cttttttgtt actgctgatc aaaaaagcaa
     9241 gccctaatgt gacaaataaa ccaataatac ctaataaata actcaaataa tcactcctat
     9301 tccaaattcc gttttatttt acctgttttg caaataaaat actaatcttt agtgaaattt
     9361 tcattacttt tttagagtga aacgcattca ttttccatta gaaaataaag cgaacaaaat
     9421 aaaaggttta aaaagtgtaa cgtcttagta aagtctataa aaaaacgaaa ctatcgatta
     9481 agctaatagt ttcgttttca acatttataa gtaaactttt tctattggta atatagattt
     9541 tcggaagggt agacaggtgc gcaggtaata tcgattccta gttttttcaa tgtttgttca
     9601 tcatttttat tgaccatcac agtagagtgg gcttgtgctc cctcaagttc agctagttta
     9661 tcataagcaa cttgagcgat tggattcgtt actgcactga ttgataaagc gatcaagact
     9721 tcattgatgc ttagtgcatc caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt
     9781 gtttcaagaa ctaatggtga caataaagga atgtcatctg cgatatgggc taaatgtttt
     9841 actgcattta agataactgc cgctgacgca tccatcaatg aacttgtacg cccggtaatg
     9901 atgacttcat ctggtaattc gattgcaatc acagcagaag gatcttttgt aggactttgc
     9961 gcttgaatct tatctgcata gtcatgggca gggactacca cgtttcggtc ttcaggacgt
    10021 aaaccgactt cttccataat cagtttgatt cgattaactg cttcttcgtc gatcaatcct
    10081 tttttgaagt cgcattctgt ttggaaataa cgacggatga tttcttgttt agaggcttct
    10141 tggacaacgg tatcatccac gatcccaaat cctacacgat tcacacccat gtcagtagga
    10201 gattgataaa cagattcttt accagtaatt ttttcaataa tacgtttgat tacaggaaat
    10261 gtctcaacat cccgattata gttcacagct actttattgt aggcatcaaa gtggaaggaa
    10321 tcgatcatat tgacatcttt tagatcgact gtggctgctt cataagcaat gttcagcgga
    10381 tgtttcaacg gaacattcca tactgggaaa gtttcgaact tggagtaacc agctgcatgt
    10441 cctttttggc tttcatgata cagttggttc aaggaagtag cgagctttcc acttcctggg
    10501 ccaggagctg tcactacaac gattggttta gttgtttcaa tatactcatt ttttccaaaa
    10561 ccatcttctg agacgatttt ttctacgttg actggatagt cttcgatttc tgagtgttta
    10621 tagaccttga tgtttcgtct ttctagttta ttgataaaga ctttcgtggc aggctgtcca
    10681 ctatagcgag tgataacgac actgttgatt aataagccat aaccacgtaa ttcatcgatt
    10741 aaccgcaaga tatccatatc ataagtaatc ccataatcac cacggatctt attacgttca
    10801 atatctccag catagacgca aatcagtatt tcagcttgat ctttcaattt ttgcagcaat
    10861 ttgattttcg catcttcttc aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact
    10921 agcttcccac caaattctaa atataatttg tcatagtggt tcactcgttc caaaatatac
    10981 gctgactgct cttcaatata tttttctgaa tcaaaaccaa tttttttcac taggttccct
    11041 ccttaagtac taaagcattt tttcattttt ttgaggattc gtcaactgga agagcgaaat
    11101 tcctcaaaaa aacaaagaaa atggtttaaa ccaacttgtc tagatagtta ttcattggta
    11161 tgatagtttt tgataaggaa ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata
    11221 taaacactat atccgttcga tttttaccgg ttatgaaaga atgcttgaag atcccgaatt
    11281 ggaacaattg acgtttactt atctaaacga agaaacgcag ctaactcgtg atgagcatca
    11341 gcgcattcat ttcacaacaa gagatctgcc tagtaaataa aaaataagat aaaggtcatc
    11401 ggattcgatt ctgtcctatc agatggaaag actagttgat cgaaaaaaag gctgtaccaa
    11461 attttggtgc agcctgtttt atggagcatt gcccgctctt ttttataaca attttttaca
    11521 gaaaatggta ctattgaagt attaataagt gaaaaacaga tgagtaagag gaggcaagta
    11581 gtatgaaaga gccagataca aatgcaaatt atgagccaga cgaactagag gagacgaatg
    11641 cggatcagga tacaggacaa gaatcgtctt ctttttacga tatcaaagaa aaagcaagca
    11701 tgaatctttc ccaattatta gaagacttac aggcttttga aaaagctgtc cgcgaagaag
    11761 atatccctga gatctaccgt ttatacaatg gccgattgaa tgaagaatta aaaagaagct
    11821 caaatgaaaa tcacgagatc gatcaattgc tgatgcgtaa gatccatgat cgttttttac
    11881 aggaatttcc ttttatggaa caagtagaaa acatctcgcc aacgatgagc tattacaaaa
    11941 tcggcactta ttaccatgat cggcccacgg tcggtattga tgcaagtctg ccagaaattt
    12001 ttgttttgcc taagatcgat gaagagtggg agaaatattc ccaaccggag tcaacggact
    12061 atgacaaaaa aatcaatgag ctagacgcaa aggtgatcac tgctcagaca gaaattgaac
    12121 gtttagatga acaaataaaa gaaatcaatc gacaaatgac tgatttgaac gacaataaag
    12181 gattcttgaa tcgtaaaaaa atcgaggaag aaattcaaga attagagaag aagaagcaag
    12241 tactatctaa tgaaaaatta ggctggcttc cttatattga gacgcctgaa acaatccagc
    12301 agcaaaaaga agcattgaag caagaagcaa gagcagacca gcttcgtgca gccatcgtag
    12361 aaaaagaaca aaggcaaatc agacgctatt tcggtagcaa agagggattc ggtcaagcaa
    12421 tccatgaatt cttgatgaat tatcttggca atgagaatcc taataatcaa tctaatgaag
    12481 gaggttccga gtatgaatga acacgatgaa gcagaatgga caaaaaatga agaacaaaca
    12541 gaagatcatg aagaaacgaa cacttcattg gaagaacaaa caagtaatcc tctggatgac
    12601 acagaagaaa tccctatgct agaggaaaca aaaatacacg aaaaagaaaa agtaactccg
    12661 acgaatacga atactgcaga aaacacccat gcttacgaaa taactgaaaa tcccgggtat
    12721 ttagaaggaa aagagcaaga tcatcaagat atcgtcagtt tgattgaaac agcggaagca
    12781 gaattagcgg atttacgtat gagaaaagat ttgatcgaat cagattttcc acaattatta
    12841 gaatcatatg atcgttttat attgaatgaa gaaccaatct ccacgatcgg aaagacaagc
    12901 ctgatcgaat acttcacgta tgaattactg aaaccgttga atgatattgg tctggaacgg
    12961 tcaaattcag cttatgacac atggaaaaca agacattttt cgatcaatta tacgttgaat
    13021 ggacaaaacg aattagtctt ttcctttgta ttaccgacca tcgatcaacg ataccaagtg
    13081 gaatcgatga gtctcctaaa cgtatcacca gagacaatgg aaatcaatgt ccaagatgac
    13141 cgggtgcttt ctttgatccg acactggtca gtggatcgag tattctctgc agggcagatc
    13201 acgatattca atcacaaatt gaatcaatta ttagcccatg caagaacact tggttttacc
    13261 gttaatcaga cattactaga caatacgaag ccacttcgtt tgaatcttca gagcgaattt
    13321 gaattgacga atgaagtatt ggatgatatt tttatcgtgg caatgaaaaa tccatcctac
    13381 gatatggaaa agatcaacga aactacttat caagtacgat tagataaagg acagtcttta
    13441 acgataacaa caaacgaaaa agatcaaaca gtcttgtcga tctcatctgg agactaccca
    13501 cgatctgtca ttgatttctt tatcaattac gaattcttgg tcccattaat ggtgagaaag
    13561 tcctgattgg ataagttgga ttaccagatg tcattcagat aattctgttc caaaggactt
    13621 gcggcagtta tccagaataa ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa
    13681 taacttgctg atgaaggagg gatttcatta ggaatgtcca caaataaaat agcacagctt
    13741 agcttattgt ctgctgcttg tgtagcaggt aggatcgctt ttcaatttat tccgaatttc
    13801 cagccaatga ctgctatttt tttatttata gtactttact taagcttaaa agatgcgtta
    13861 attgtcatga gtctttctat tgcgatcagc agtttttatt ttggtgttgg accatgggtc
    13921 atcggccaat ggatcagcta tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga
    13981 aaaacagtgc aaaggaatct atggttaaag ggagtctgtt tttttctggc gggcatggtt
    14041 tacggaatag gtatgaccgt ttttgatacg ctgttatatc gtttgcctca accttggatc
    14101 tattaccttc aaggggtgtc gtttgatttg atgcacagta taggcaatgt tgtgttcttt
    14161 cttgtttttc tgccagtcgt caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa
    14221 aataatttat agtttgcttg caatgagtgt cagtatactc attttgacaa gttgcagtac
    14281 aacctataca caatcggctt ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac
    14341 aatcacgtta caagaaaatg gtcaaaactt ttcggaaaaa gaagtgttct tcaaaaaagg
    14401 cgatgattta ctgagtattt tgaaacgaaa ttttgaagtc aaagaagata acggcttcat
    14461 cacttcgctt gaagggcact cacaggatga gaaaaatcaa ctctattgga tgtttactgt
    14521 ggacgggaaa agtgctacta ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt
    14581 cattttcaat ttgtctaaac tataagtttc ataaaaaact gttaaagggc gaatattgcc
    14641 ataattcagt gagttcagtt gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag
    14701 cggaatgttt tccgtaattg ctgctctagg ggctaatatt ctagttgcta tcagcaaatt
    14761 tgtcggctat gcaatcagtg gcagtgccgc catgctaaac gaaagcatcc acagtatcgt
    14821 tgattgcagc aatcagattt tgctgttatt cggtgataaa caagccaata aaggacagag
    14881 cgagttgcat caatttggtg aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat
    14941 gatgcttttc ttcggcggtg gggcacttgg ggtgatggaa gcttttgaaa agttgacgca
    15001 tccatcccat gaagtgggta atccgatcat tgttatagca atactgttat ttggtttggt
    15061 aatcgaagga agttcgttac gcgtggcaat gaaagaaatc aaagaattga atactgaaaa
    15121 actttccacg atgcgctttt tgcgcgaaag tcgacacagt gaaatactga ttatttttac
    15181 ggaagacttg tgtgctgtaa tcggtttggt cttggcttta gcaggaacaa tattgactta
    15241 tgcaaccggg attgctgcat ttgatgcgat cagcggattg ctgatcggtt tcttgctgat
    15301 gggagccgcc attttcctag caaaagaatt ttatagtctg atcatcggtg aaagcgtgac
    15361 agcaagcgat ttatcaaaaa tcaagttagc ttttgaacgt cctgagatca gtcggctgat
    15421 cgatataaaa acagttcatt taagtccaac ggagatttta gttgcagcaa aagcagatat
    15481 cgttggtaac aaagagaatg aagcttactc ggtcatcaat gatattgaaa aaaatatgcg
    15541 tcagtctctt cctgacaaga agatgtatat atacattgaa acagataaat atgacccaaa
    15601 ttattctcgt aaataaaacg tttattcatg aattgaagca aaaatgagaa aaatataatc
    15661 ttgtttttaa ataaaacggc tacaatttag cgaatttatg ttacaatgag tccgctgttt
    15721 atttgttgta attaaaggag atattttaat gaacaaaaag aaaagtgcgc catggcttca
    15781 agctttgcat gcagtgatgc cactttgtat aagctatata ccagtaggat tagcttgcgg
    15841 agtattgttg caaaaagttg gatttgatcc tctcttatca ggactgttat cgattctggt
    15901 cttttccggt ggcgcgcagt ttttagttgc atctatgctt acgacgcaag catcatttgc
    15961 aacgacatta ttgatggttt tctttttaga attacgctat acattattag gagcaagtct
    16021 tgccggtttt atgaaaaaag aaaagcggac gtttttggct gtcttttctc aatcactgaa
    16081 tgatgaaaac tatgcggtga actacttaaa gttttcaact gacccttcct ggaataaaag
    16141 aaaggcttta tatgtcaatt ggttctcaat gactgcatgg gccggtagca atatgatcgg
    16201 taattttttc ggctcggtga ttcgagtaga tgctgacctt gtccattttg ctttgacggc
    16261 catgttcatt ttcatgttta tcatgcagat gaaaagcgca ctactgatct ttacaggttt
    16321 attctcaggc gtattagggg tcgtattcat ggtattattc caaaatactt tcggattgat
    16381 tgcagcaaca gtcattgctt catttgccgg atttatgctg gagaaggcat tcaaaaaaga
    16441 aaaagctaag aaactaagag accgcggtaa agatgtcaat gagccgctct tcaacttccc
    16501 ggaacaggag gccaatcacc atgactaaac tatatttgct acttttagtt gcttgtctgt
    16561 tcatcgtggc atatatcccg cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc
    16621 catcctggtt cagtgactgg atgaaatatg taccggtggc attatttgcg gcgttagctt
    16681 ttaaagatgt tttcattact catgaacatc tggatattgc ttggaatatc aaaatagctg
    16741 caatgattct agtagcaggc gtggcttaca aaacacgttc gatggcgttg tcagttttga
    16801 caggacttgc gtctgtcttt ttgctgtcta tgctttaaac tgttttattt tattaaaaac
    16861 agagattgcg acataagttg ttcaaactga tctatccgaa ctgcatttgc aacagtcaat
    16921 tctggatgca agtttattcg tagggataaa aaagattagt gttggcttat gatgcaagct
    16981 cttttttatt ttaggaatta gagagggatt tgtccttcta tgattttttg ctataatagg
    17041 gaagatgttt attttatgaa agaatagttg agggaattta aatgaataaa aaattgatgc
    17101 aaggaacatt ttggctaacc tttgctaatc ttctatgtaa agttttaggg gtagtctatt
    17161 tgattccctg gctaagtatg atggggaaca atcaagatgg tatgcttgct acgacattgt
    17221 ataatgttgg ctatcttcct tatggattgt tcctgatgct tggtacagtc ggatttccaa
    17281 atgcgattgc aaaaaaagta gctgtagcca caaaagaagg agataacgct gcttgtcggc
    17341 tgatttttag aagtacgatc aatatcatgt ttgtcatcgg tatcgcctct gctgcactga
    17401 tgtatttatt tgctccttta ctagccggaa tcagtccgat tgctaacgtt aataatggga
    17461 tactagctat cagaagttta tgcccatctt tgattgctat cccaattctt agtgcaatga
    17521 gaggatattt ccaaggaaaa aatgatctgc ggccttatgg aacttctttg attatcgaac
    17581 aggctgtgcg agtgatcgtt atcttggctg gtacttatta cttgcgcgta ttgactgacg
    17641 gaaccatatt agaagctgta ttgatcagta cagtcgcttc tttcttcggt gggttagctg
    17701 cgattatcca tatgtatatc gtcggacgca gaaaagactt cttcgaactt aaagattttt
    17761 tgatttccag ccgttatttc gaaagagaaa accgatctgc ctctgtctct atcattcggg
    17821 aaacactacc gtttattttt gtcggctcag tcattactat cgttcaaatg atcgaccaag
    17881 taacgatgaa accactgctt cactttttcc gtccagagat cgcagatcag caactggaat
    17941 tcttattcag ccgtgcttct gtcaatccaa ataaattgac attgatcctg atctccatgg
    18001 taggaacagt agctataagc agcttgccaa tattaagcac gatgaagaaa aaagatcgga
    18061 tacaaatcga aaaaacagta ggagatagtt tttcgattgc gttattgatt cttcttcctt
    18121 cattgactgg aatgtcttta ttagctggac cactatacac attgtttttt ggctatgatc
    18181 ctgaaagtgt cggatatttc caaatggctt tgctagcttc tttgtttttc tctttattca
    18241 cgatcctatc tacgatgctt caatctttga atcaccatcg ttttgcgatc cgattaactg
    18301 tagaagcaat tatcctgaaa gtcatcttcc aagtaatcgg attaggatta gttggcggat
    18361 acgggatgag cttatcaaat ggcttagcat tcggtattgt ctttgttcgc ggctatcact
    18421 ttatgtgcaa agaataccga atagcacctt tagcaaagat cagcaatttt ttcttaaaaa
    18481 ctttccgctc caccctgatt atgcttgcag tatgttttgc cgttttcttt ttgctgaatc
    18541 agcgattgtc aatggaatca aaaagctatg caatgattta ttgtgtggct gtaggaagta
    18601 ttggcggctt agtattttta ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa
    18661 atgttaaaca tcatagcaga agcaaagaat aggttaacta atcgtttttt gctgtcactt
    18721 gaaaaacggc aactcgtatg attacgcttg tcgtttttct attttccaat tttagtttca
    18781 tatctaatct tacttatgat atagttataa ggtctggaaa aattctttga aacgaggaaa
    18841 ttaaacacat gaaacttgca gtagttacag atagttcggc atatctgccg gaacgtatca
    18901 agaaccatcc cgacttattc gttattccaa tccctttgat tatggatggg aaaatttata
    18961 acgaaggaat cgatatcgaa gcggatgaat attatggact tttaaaaaac agtaaagatt
    19021 ttcctactac ttcacaacct gtcgtagggg aagttttggg tttatatgag gaattgaaag
    19081 agaaagggta tgacacgatt ttaagtatcc atctttcttc agggatttct ggctttgtca
    19141 acacattatt tgcgatgaaa gatgacatca gcggagtaac ggtcattcct tatgactcta
    19201 agatcactag tatgccgatg ggacacatgg tagaagctgc cttagactta aatgatgaag
    19261 gtcagtccct tgatgaaata cttgcacata tcgatcgaat ccgagataat acctatgctt
    19321 atctgattgt agatgatttg aacaatttag tacgaggagg acgtttgaca aatggtgcag
    19381 cgctgattgg cagcttatta aaaatcaaac cgatcctgac attttctgaa gggaaaattg
    19441 ttttattcga aaaaatccgt tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg
    19501 gtagaagaaa taacgaaata caaccgccag tcaaacttta cgtgatacat gcaaataatt
    19561 tagaagtagc gattgcggag aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg
    19621 aaattggtca tttcggacca gtcattggta cacatttagg tgaaaaagcc atcggcattg
    19681 ccatttcagc gcaataattc cttttattca aaaaacatac caaaaata
//
LOCUS       DAPCMT010000041        19661 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPCMT010000041 DAPCMT010000000
VERSION     DAPCMT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.041"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..504
                     /locus_tag="RD743_001239"
     CDS             <1..504
                     /locus_tag="RD743_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDU0563064.1"
                     /translation="FQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADF
                     RMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNE
                     VIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVR
                     YMFNKIT"
     gene            602..790
                     /locus_tag="RD743_001240"
     CDS             602..790
                     /locus_tag="RD743_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDU0563065.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(848..1201)
                     /locus_tag="RD743_001241"
     CDS             complement(848..1201)
                     /locus_tag="RD743_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0563066.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1732..2154
                     /locus_tag="RD743_001242"
     CDS             1732..2154
                     /locus_tag="RD743_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDU0563067.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2151..2381
                     /locus_tag="RD743_001243"
     CDS             2151..2381
                     /locus_tag="RD743_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0563068.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2879..3079
                     /locus_tag="RD743_001244"
     CDS             2879..3079
                     /locus_tag="RD743_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDU0563069.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3106..4299
                     /locus_tag="RD743_001245"
     CDS             3106..4299
                     /locus_tag="RD743_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0563070.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4367..5932)
                     /gene="guaA"
                     /locus_tag="RD743_001246"
     CDS             complement(4367..5932)
                     /gene="guaA"
                     /locus_tag="RD743_001246"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDU0563071.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5991..6914)
                     /locus_tag="RD743_001247"
     CDS             complement(5991..6914)
                     /locus_tag="RD743_001247"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDU0563072.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7110..7715
                     /locus_tag="RD743_001248"
     CDS             7110..7715
                     /locus_tag="RD743_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDU0563073.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8023..9039
                     /locus_tag="RD743_001249"
     CDS             8023..9039
                     /locus_tag="RD743_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDU0563074.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9180..10481
                     /locus_tag="RD743_001250"
     CDS             9180..10481
                     /locus_tag="RD743_001250"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDU0563075.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10527..11189
                     /gene="deoC"
                     /locus_tag="RD743_001251"
     CDS             10527..11189
                     /gene="deoC"
                     /locus_tag="RD743_001251"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDU0563076.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11210..11608
                     /locus_tag="RD743_001252"
     CDS             11210..11608
                     /locus_tag="RD743_001252"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDU0563077.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11674..12756
                     /locus_tag="RD743_001253"
     CDS             11674..12756
                     /locus_tag="RD743_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0563078.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12968..14530
                     /locus_tag="RD743_001254"
     CDS             12968..14530
                     /locus_tag="RD743_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0563079.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14523..15656
                     /locus_tag="RD743_001255"
     CDS             14523..15656
                     /locus_tag="RD743_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0563080.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15656..16612
                     /locus_tag="RD743_001256"
     CDS             15656..16612
                     /locus_tag="RD743_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0563081.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16711..17877
                     /gene="deoB"
                     /locus_tag="RD743_001257"
     CDS             16711..17877
                     /gene="deoB"
                     /locus_tag="RD743_001257"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDU0563082.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17901..18719
                     /locus_tag="RD743_001258"
     CDS             17901..18719
                     /locus_tag="RD743_001258"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDU0563083.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18736..19443
                     /gene="deoD"
                     /locus_tag="RD743_001259"
     CDS             18736..19443
                     /gene="deoD"
                     /locus_tag="RD743_001259"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDU0563084.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6281 a         3537 c         4233 g         5610 t
ORIGIN      
        1 tttcaaaatg cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg
       61 aatgtgacgg attgtaaaat ctgttttaag tacggtttat actatagccc tgttagtact
      121 ccagcagatt ttcggatgct tactcctatt gtactggagc aagcctttag aaaagctgga
      181 acagaattgt tagagccata tcttagtttt aaagtttatg caccacagga atatctttca
      241 cgggcatata acgatgctcc caaatattgt gcaaatatcg taaatactca actgaaaaat
      301 aatgaggtca ttattattgg agaaattcct gctcgatgta ttcaagatta tcgcaatgat
      361 ttaacttttt ttacaaatgg gcttagtgtt tgtttagcag agctaaaagg atatcaggtt
      421 accactggcg aacctgtttg ccagacccgt cgtctaaata gtcggataga taaagtaaga
      481 tatatgttca ataaaataac ttagtgcgtt ttatgttgtt atataaatat ggtttcttat
      541 taaataagat gaaatattct ttaatataga tttgaattaa agtggaaagg aggagattgt
      601 tattataaac tacaagtgga tattgtgtcc tatttgtgga aataaaacaa gactacgaat
      661 acgagtggat actatactta aaaatttccc tttatactgc cccaaatgta agaacgaaac
      721 tttaattaat gttcaaaaaa tgaatataat aacaatcaaa gagccagacg ccaagacgca
      781 gagccgataa tttgagaaat gaaactctca tcttatcggc tctttttgtt tatctgaatt
      841 ttactgacta gccttcaata tttcttgctt cattgatacc ttttgctagt gcttccatta
      901 aggatagttc tttgtctgta aagctatcca tgtatttttc tacctgtaac cttcgagtgc
      961 ttttaatcaa attatctgta ggtaagaaaa attcatcaat agatacatgg agtaatgata
     1021 caagatcata cagcacttgt gtacttgggt gttgtccttt gttttctata tttgttaaat
     1081 aacgtgggtc aatttcaatc attgttccca cttgttcacg agtgagaccc cgtttcattc
     1141 gagcctcttt aatcgctaaa ccaacggctc taaaatcgta tttatcttct tttttacgca
     1201 tactaaatca cctctataca ttttactgtt cctatataac cataaacagg tatagaaaaa
     1261 catgttatat agtttatgag ttcctataga acactgtgga atttattaat gcgatgaatc
     1321 caatttcaaa tagaaaggtc gctgatttta tttacctata caactttata aaatttagtc
     1381 atactcctag aaagtagata aaattggcga cttaatggag ggaaaagtcg ccatgagaaa
     1441 gataatctgt catctagttc tcttaattaa gttcttatca aaaaaactta tccacctgta
     1501 ctggatagat atgaccatca atgtggtttt gcagattaca tagatagaaa aatcaaagca
     1561 tatgaacaga gaaaacaatc tgtttatgtg cttttttggt cacttaataa atatctttta
     1621 ggatttctct tcatttatgc aacaaatccc ccgttcgtgt cggggtttag tatgaaggga
     1681 gataaagaag cacatgccac tccctttcac gacagaaagg gggtgaaaag catgaaacca
     1741 tcttcttttc aagaaaccat agaaaatcag tttgactata tctgcaaaca agttattgaa
     1801 gatgaacgaa aagattactt caaacatcta gacagactat cagcaaaaga agtctcgttt
     1861 tctgatatag ataactacct tgtcaactct ttttctactg ttgatacgta cgtcactgat
     1921 ttccaactat ttaaattatt tggattaaga atcaatgttg aaagtgattt attaagtgaa
     1981 gccctgcgtc atctttcaga gaaaaaacgg aacattattt tacttcatta ctttatggat
     2041 atgagtgatg tggagattgc ggagttacta aatttgaatc gttcgactgt ttatcgtcat
     2101 cgaataagtg gactagccat gattaaagaa ttcatgaagg agtgtaaaga atgaaaacag
     2161 cttatccaag ggttcccttc cccctcattg taaaggcgac agatggcgac gtggaagcaa
     2221 ttaatcagat tgttaaacat tatcgagggt atacctcaaa acgttcatta cgtcgcatga
     2281 cagacgaata tggaaattct catatggtta ttgatgaaac cctacgtggc agaatggaaa
     2341 cgaaactcat ctctaagatt ttatcgttcg aaattaaata gtcccatttt ttcgtggaag
     2401 cgtgtcgaat cgtatccacg cttcccgatt tgggaggttt gttattccat caaagcatat
     2461 tgtgtgttca gtaggttttg ataggctaac aagccaaacg ttctttgaca accaaataaa
     2521 ggcaatcgaa tacgtttcgg taagaaaaga gctgatgaac taacacgcca tgacctacaa
     2581 aattagtgag cgattcatgt tagtaattcg aaagtaatct ttagcaggat tgctgccatg
     2641 actttcttat cgtgataatg atactcccgt atagccatag tccgagcgtt agaagcgtca
     2701 caggcaatgg gtacggctac atgagaacca tgcagaagtg aaattcctgt gagctttgct
     2761 agagctgttc gattgctaag atattcttct cttgtcagtg tattattata aaactatttg
     2821 atataattat aggtgaatat tggagttttc ctagcctata attgggaaag gagtaataat
     2881 gataacggtc acaaaaaagg atttagttga gctaggatat ggaacttctt tttcagcaga
     2941 catcataaaa aaagcaaaag aattgatgat tacaaaaggg catatctact atcaatctcg
     3001 aaagctagat agagtccctc gtgaagctgt tgaagaaatt ctaggaattc ggttttcaaa
     3061 tgaaaacagg tctgagtagt ttttgcacag taaggagtaa aaactatggt taaagaccca
     3121 ataaaaaaag caaagaatgg aacctattat tttcgtgcaa atctaggtta tgattctaaa
     3181 gggaaaaaaa ttcaaaaata tcgtagtgga ttcactacta aaaaagaagc aagagaagaa
     3241 tattcgaaac tgctattgat gaaaccagaa gaattaagtg agaataagga taaaatgacg
     3301 tttaaacatt atatctttga aattttcctt ccatggtata agacacaggt aaagctcaga
     3361 acatatgaaa atcgcttacc aactattaag aagcatttct cttattttga taaaatggct
     3421 gtttccgata ttgagcctat tgatgtgcaa aattggcaac taaaattatc aaagaagtgt
     3481 aagtcttctt atgtaagagc tgttcaagga ctattttcag ttgctatgga tcgagcaatt
     3541 gtccttggga taaccaccac aaatccatct aagattattg gcaatgtgaa aaagcaaaag
     3601 tctaaaattg aattttggac aaaagaagaa tttgaaaaag taatctctct aatttacaaa
     3661 gaagattact atcaacactt tttatttatc tctctgtggt ttttatttat gactggaatg
     3721 cgaattggtg aagctactgc cattcaatgg gaagatattg attttgactc aggagtacta
     3781 accatcaaca aaacactcta ttacaagaat caatcgaatt atagatttgt tgaaccaaaa
     3841 acaaaggcga gcgttcgtca tattgtatta gataaatgca cattaactta tttatccgaa
     3901 tggaaggaag tacaacaaaa tctaattcag acagactttg taatgagcta taacggtatt
     3961 cctacccaaa aacatacact atcatatgct attgaacgct atgctaagct ggctggtatc
     4021 catcgaatca gaatccatgg gttacggcat tctcatgctt ccttgttaat cagtatggga
     4081 gaaaatccac ttatcattaa agatcgtcta ggacacgaag atattgagac gactttagga
     4141 acgtatggac atctttatcc taacagtaat ttcgaggtag ctaacaaatt aaatggagcc
     4201 atttccttta aagaagcaaa acaaaattat gatacttcac caaagaatca atttactgta
     4261 gactacttgc gaaacaaaga aatgaaaaaa gtgcaataaa agtgcagtaa aaagataaga
     4321 agagccagaa acccttggta taagaggatt tctagctctc ctacaactat tcccactcca
     4381 ctgttgctgg tggtttagaa gtgatgtcat acacgatacg gttgacgtgg gctacttcgt
     4441 ttacgatacg tacagagatt ttttgcaaga cttcccatgg gatacgggca aagtcagcgg
     4501 tcattccgtc gattgaagtt acagcacgga taccgactgt gtagtcgtaa gtacgtccgt
     4561 cacccataac accgacagag cggatccctg gaagaacagt gaaatattgc cagatatcac
     4621 ggtcaagtcc agcagctgcg atttcttcac gcaagatagc atcagattcg cgtacgattt
     4681 ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc aagaccagga ccagggaatg
     4741 gctgacgcca aacgattgaa tcaggcatgc ctaactgagt ccctaattca cggacttcgt
     4801 ctttgaataa tgtgtttaat ggttcgatca attcaaattg catatcttct ggcagtccac
     4861 ctacattatg gtgagatttg atcgtctgtg ctgtttctgt accagattcg ataacgtcag
     4921 tatacaacgt tccttgtgct aggaaagaga cgccttcttc accagcaagt tttgttgctt
     4981 cgtcatcaaa aacatatacg aattcattcc caatgatttt acgtttttgt tctgggtcag
     5041 atacaccttt caacttgtct aagaagcgat ctcttgcatc gactttgatg atgttcaagc
     5101 cgaattttcc gcctagactt tccattactt gttccgcttc gcctttgcgt agcagaccgt
     5161 ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga accccaacga
     5221 cactagaatc aacgccacct gaaagaccaa gcaagacttt tttgtcacca acttgttcac
     5281 ggattttttc tacttgcata tcgataaagt tatccattgt ccagttacct tcacagtgac
     5341 aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga cgtacttctg
     5401 catggaattg gataccataa aaattacgtg ttgtgtcttg gatagaagca atcggacaat
     5461 ctttgcttgt tccgactgtt tcaaagcctt caggaacttt agttaccaag tcaccatggc
     5521 tcatccatac tgtttgttgt tttggtgttt ctttaaacaa gacagcttga tcgcttgtta
     5581 cttctaacaa ggctttccca tattcgcggt ttttagcagg ctctacggaa ccgccaagtt
     5641 tgtacgtgat caattgcata ccatagcaaa tccctaatac aggaatacct aaattgaaga
     5701 tctcaggatc gatcccaaat gcattctcat catatacact gtttggtccg ccagaaagaa
     5761 tgattccctt tggattcatc gcttttactt cttctgcagt aatgcggtgg ctcaataatt
     5821 cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt
     5881 agtctaatac gataatcttt tcaacgttcg tcatatcggc aacgtttgtc aaatttatta
     5941 cccctatccc tttaaatttt atctcaaaat agtcaaacta ttttaaaatg ctaatatttt
     6001 cgcatgaaaa tcgaatcgat gatatgattt tcagttttat ggagaattat atcggctctt
     6061 cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat tcacattttt ccatacatct
     6121 tttgccattt taaaggcttc ttcacgatct ccgatagcaa aacgataata ataattctct
     6181 ggattttgga aagccgtatc cagtaacgca ccaaaacgat ccaaatacca tttttcgatc
     6241 agcacgggat cggcatcgac atagacagaa aaatcaaaaa agtcgctgac gtagatttgc
     6301 tgattcgcag gtaattgaag cgtattgatc ccctcaacga tcaagatatc aggctgctgg
     6361 atcaattcgt acttgtctgg aatgatatca tacacatcat gtgagtaaac aggtactttt
     6421 atctcttcct gaccagattt tacacggttc aagaaatcga tcagcatctc catatcataa
     6481 ctttcaggaa atcctttacg gtccatgatt ccacgctctt ttaatatacg atttggataa
     6541 agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc
     6601 gtctgcagca gtctggctgt tgtacttttt ccaacggcta cacttccggc gatcccgata
     6661 ataaaaggcg gtacttctac gtatttatga agaaataaac ctttactcaa gaacaaagat
     6721 tcaaattctt tcatataaag ataaatcaga tgagcaagag gtacatagat atctcgaaca
     6781 tcctgcatgg aaatgcggtc attcaaactt ttgatattat ctagttcatc ttgtgtcaaa
     6841 ggtgcttgcc cattacgata aaattctcgc cattcttctc ttgccacacg gtagtagttc
     6901 attggatcat tcatattagc ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa
     6961 ataaacgcaa accccaccac ttaagagaga ttgccgatct ttttgtataa tctttataat
     7021 agaaagaaac ggagtcttat tcttgacttg cgcacggata cataaaagga tagactgttg
     7081 tcatgaaaga aatgagaagg atggaagaca tgacaaatca ttactattct gaacgcccag
     7141 atacggcgca cgattttgaa caatgggctt ttgagttaaa aggcagaacc tttcaatttg
     7201 taacagattc tggtgtattt tcacgtgaaa cagtggatta tggttcacgt gtcctgattg
     7261 atgctttcga ttgggaagag ttacctgatg aaggaaagat cttagatgtg ggatgcgggt
     7321 atggaccgat tgggttagct ttggcatttg cgacacagcg gtttgttgaa atggtcgaca
     7381 ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg
     7441 ttgatatcca tcaatcgaat atctatgaag cagttcatga agaaacctat gcagctatcg
     7501 taagcaatcc gccgatccgt gctggaaaaa aagttgtaca tgaaattttg acaggcgcat
     7561 atcctcggtt gaagaagggc ggtacactga caattgtgat ccagaagaaa caaggagcgc
     7621 cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc acaaaagata
     7681 aaggatatta tatcatcaaa agtgtgaaag aataactagc tgaaaaatag tggagcgacg
     7741 aaagagtgga actgggcgtg catgtacggg tacgtatcag ttcttttttt gttgagaagt
     7801 ttttaaaaac aaagaactat tttttcttca aaaagaaata gtaggaacag agtcgttgtt
     7861 ttttacgata aaataaataa gatacagaac ttgtatagaa ggctgccaag tcggtagata
     7921 aaggaggttt ttatcaaaaa acatactttc cttttttctc tattcttttc ttgggggttt
     7981 tggtacactg atagggaatt tagatagaag ggagccaata ggatgaagct gaccatcaga
     8041 gatatcgcag aaatggcagg cgtatcagta acaactgtct cacagatttt gaataacaaa
     8101 ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat
     8161 tataagcccg attatttcgc ttcaaatgtg atcaatcgac attcaaagac catcggaatg
     8221 atcgtccctg atgtcactga tttcttcttc tccaaactga tcgaaggtgt agagagctat
     8281 ttgaatcctt tgggctatgt gatcatgtta tgcaattctc gtcatagcca agaaaatgag
     8341 atcaagtatt tacaagagct ttcccaccgc tcagtggacg gaatcttgct ggccacacca
     8401 aatattttac ctgagaaata tgcattagac agcggttttt atcaaaaaat gccaatcatt
     8461 ttaatcgatc gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag
     8521 ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc atacgaagat cggcatgctg
     8581 aaggaaacca ctggatacta tcaattagaa gaacgtttca acggatatcg tcatgcgctg
     8641 aaagatcatg gcctgccttt taatgggaaa tttgttgaac atggagagtt gactgtccaa
     8701 ggtggatata ctgcatcaaa aaaattactc aaacataaag atatcacggc tattttttgc
     8761 gggaatgatg ccatggcgat cggatgctat caggcgatcg atgaattggg caaaaaaatc
     8821 ccagaagata tctcagtcat aggattcgat ggattaaagc tgtcagaata catgattccg
     8881 caattgacga cagtgcagca gccaagtttt gatattggat tttatgctgc acgtttcctg
     8941 atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta
     9001 attattcgtg agagcgtaaa agtattgaca aagggctaac tcggcggtat attgcttatg
     9061 taagcattta caaaggctag ttagtgcttt ttttattttt agtaaaccag tttactaaag
     9121 tggtttacct ttcctaaaaa taatgtataa taccgaatga agacaaggag gatcaggcaa
     9181 tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa
     9241 tcgagtacat cgtcacgaac tatacgaatg gaaagattcc agattatcaa gtcagtgcat
     9301 tattgatggc aattttttat caagacatga caaatgaaga aatcacgaac ttaacattgg
     9361 cgattgctaa ttctggtgat gtcatcgatt tgagttctct tgaaggaata aaggtagata
     9421 agcattcgac aggtggcgtt ggtgacacga ccacattgat tttagccccc ctagtagcta
     9481 gtgtcggagt gacagtggct aaaatgtctg gacgcggtct aggttacaca ggtggtacac
     9541 tagacaagct agaagcaatt cccggatttc agatcgagct ttcggatgaa gaatttgtac
     9601 gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg
     9661 acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt tgattctctt ccgctgattg
     9721 ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga
     9781 ccactggaga cggtgctttc atgaagaaca tcgaagatgc aagacgtctg gcaaagacca
     9841 tgacgagcat tgggaaactg gctaaccgag aaacagtggc ggttatctca gatatgtcag
     9901 aacctcttgg cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc gaaacattac
     9961 aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc cttaggaagc caaatggttg
    10021 ttcttgctgg caaagctaag acaaccgatg aagcaagaac gttgcttcaa gaagctctgg
    10081 aatcaggtaa agcgttagca aaattcaaag aaatgatcca aaatcaaggg ggagacccag
    10141 cgatcgtgga acatccagaa cgaattttga ctgcacgcta cacaatggag cttcctgcta
    10201 aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc
    10261 taggtgctgg gcgtaaaacc aaagaagaag atattgatca cgctgttgga ttgaaactgc
    10321 ataaaaaaat tggtgacact gtgacaaaag gtgagtcact attgacgatc tatagtaacg
    10381 acgaagaaat cacttctgtc attgaattat tatataaaaa tattgaaatt ggtgaatcgg
    10441 caatgaagcc aacgttgatt catgacatca tcacagaata aaggaaaaaa gcggacttta
    10501 ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg aatgatcgac catactattt
    10561 tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat cgaagaagca aaaaaatacc
    10621 atttttattc tgtctgcatc aatccagcat gggttgcgct agcgaaagaa caattacaag
    10681 gtgagccagt agccgtatgt acagtaatcg ggtttcctct aggagcaaac acacccgaga
    10741 caaaagcatt tgaaacaaca gatgccatca ataacggtgc agatgaagtc gatatggtca
    10801 tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag
    10861 ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat tattgaaaca tcgttattag
    10921 atagagaaga aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca
    10981 aaacatcaac tggtttctca acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg
    11041 aaactgtagg accagaaatg ggtgtcaaag cttccggcgg tatccataac gaagaagaag
    11101 caatggcaat gatcgaagct ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg
    11161 tatctggttc aactggtgaa ggctattaat tttttcaaag gagaagctca tggcagaact
    11221 caaacaagaa tggatcaaca cagctattga agcgttagat aaagcctatg ttccttattc
    11281 ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa
    11341 cattgagaat gcatcatttg gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc
    11401 agtatctgaa ggagaaaaaa catttacgca tctagtcgta gcggggcaca cccccgatcc
    11461 tatctctcct tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc
    11521 ggttacgcta gtaggagaca atggtgtgac gaaggcaacg accgttcgtg cactgctgcc
    11581 ttatgcattt actgaaaaag atctataact catgttcatt cagtcagctg actgttgaaa
    11641 aataaaacaa ttattttagg aggctttaca gaaatgaaaa aagcaaaatt atttggttta
    11701 ggtgctgtcg cactagcagc agggttattt ttaggtgcat gcggcaacaa cggctctact
    11761 gattcatccg gtggcaaaag ctcatcagac acaacaactg cagcattgat cacagacaca
    11821 ggtggtgtag acgaccgttc attcaaccag tcagcttggg aaggactaga aaaatggggg
    11881 aaagatcaag gactttcacg aggaaatgat ggcttccaat atttccaatc ttcaaatgaa
    11941 tcagattaca tcccaaatat tgaccaagca ttgaatgctg gattcaaaac gatttttggt
    12001 attggttata aactaaaacc agcgatccaa gaacaagcaa caaacaatac aggtacgaac
    12061 tttgttatta tcgatgatgt catcgatgga ttagataacg ttgtatctgc aactttcaaa
    12121 gacaacgaag cttcttatct tgcaggtgtt gcagcagctt atacgactga aacaaacgta
    12181 gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa
    12241 gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat
    12301 gctggtgact tctctgctcc agataaagga cgttcaattg ctcaaggtat gtatgcacaa
    12361 aacgcagata tcatcttcca tgcttcaggc ggtacaggta acggtgtatt ccaagaagca
    12421 aaatcattga acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa
    12481 tcagacgaag gcgaatacac attaaacggc gaaaagaaaa acttcacatt gacatctaca
    12541 ctgaaagcag ttggaacagt agtagaagat ttggcacaaa aatcagcgga cggtaaattc
    12601 cctggcggcg aacacactgt ttatggattg aaagaagacg gtgttggtct aacagaagga
    12661 caactttctg acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt
    12721 gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc atttctatag ccttgttaga
    12781 cacatgtttg acaaggctat ggtttgatta gaacaagaat tgttcattta tccaagctgc
    12841 cttgtgacag tttggataag ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa
    12901 cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt taggatgtga
    12961 aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag aatattacca aagaatttgg
    13021 aacattcaag gcaaatgaca acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt
    13081 gcttggagaa aacggtgctg gcaaatctac attgatgaac gtgctttccg gattattaga
    13141 gccgacatca gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa
    13201 agccaaccaa ttagggattg ggatggtcca tcagcacttt atgcttgttg atgcctttac
    13261 tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc ttgaccataa
    13321 aaaagcacga aaagagatcc aaaaagtttc tgaacagtat ggattgtcag tcaacccaga
    13381 tgcttacgtt cgtgatattt cagttggaat ggaacaacgg gtagaaattt taaaaacact
    13441 ttaccgagga gcagatgtac tgatttttga tgagccgaca gctgtattga cccctcaaga
    13501 aattgatgaa ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt cgatcatttt
    13561 gattacgcat aagttagatg aaatcaaagc agtagctgac cgctgtacag ttatccgccg
    13621 tggaaaagga atcggtacag tcaacgttaa agacgtaacc tcacaacaat tagccgatat
    13681 gatggttgga agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt
    13741 cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa
    13801 cttgaactta gaggttcgtg ctggcgaagt acttggtatc gctggaatcg atggaaacgg
    13861 gcagtcggag ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa
    13921 gctaaaaggg gaagacatca ctaataaaaa accccgaaaa atcactgaac atggtgtagg
    13981 acacgtgcca gaagaccgtc ataaatacgg gttagtccta gatatgacat tatctgaaaa
    14041 tatcgcacta caaacctatc atcaaaaacc ttacagtaaa aacggcatgc tgaattattc
    14101 agtaatgaat gaacatgcca gagaattgat cgaagaatat gatgttcgaa caacgaatga
    14161 acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga
    14221 agtcgaccga gatcctgatc tgttgattgt tgcaaatcca actcgtggac tggatgtagg
    14281 tgcgatcgaa tttattcata aacgtctgat cgaacaaagg gacaaataca aagcagtgtt
    14341 actgatcagt ttcgaattag aagaaatttt aaatgtttcg gatcgtattg ctgttatcca
    14401 tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct
    14461 attgatggcg ggctatacct tagaagaagc acgaaaagag ttagaaaagg gggctggcgc
    14521 acatgaatga tcgcagcgaa aaattccgta atttattagt gcctgtactt tcagttgttt
    14581 taggattgat cttaggcgcg atattgatgg ctgcttttgg ttttcagccc atccaaggtt
    14641 attcttctat gctagatgca gcccttggtt cgcaaagagg gataggtgaa acgttaaggc
    14701 aagcaacgcc gttgattttt acagcattag gtttttctgt agctaactca gctgggtttt
    14761 tcaatattgg gctttctgga caagcacttt gcgggtggat cgtcagtgtc tgggtggcac
    14821 ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct
    14881 tagctggtgc gatggcagcg gctgttccag gtttacttcg agcatttttt ggaacaagtg
    14941 aagtgatcgt aacaatcatg atgaactata tcttattata cgtaagcaca tttgctttac
    15001 atgaagtgat gcctgaaagc tatcgctcaa gtttggactc ttccaatatg atcagtcaaa
    15061 atgcctcact gcggttacca tggttaacac aagcttttgg cggctctcgc gtaaactcag
    15121 gattattttt agcaatcatc gcgcttattt tcgtttggct gctgatgaag aaaacgactt
    15181 taggatttga aatccgttct gttggattga atccttttgc ttctgagtat gcaggaatga
    15241 gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca gggcttggcg
    15301 gtgtggttga aggattagga acgtatcaaa atttctttgt tcaaacgact tcgctatcta
    15361 ttggatttga cggaatggct gtttccttgc ttggttctgg atcagcaatc ggtattcttt
    15421 tatcagcgat cttgttcagt attttgaaga tcggtggact tggcatgcag acaggagcag
    15481 gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat tatcttcttt gtcgggatca
    15541 acttcctgat tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa gaagagatcg
    15601 ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa ctataatgtc
    15661 gactatcgaa ttaattgcct tgatccttac ttctacactg gtttattcaa caccgctaat
    15721 tctaacgtca ctaggcggaa ctttttctga acgaagcggg atcgtaaacg tagggctaga
    15781 gggaatcatg gtcatgggtg cttttagttc catcgttttc aacttgacga tgagtccatc
    15841 acttggttct atgacaccat ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt
    15901 ttctctgatt cacgcagtag caacagtcag tttgcgtgct gaccatatta tcagcgggac
    15961 agtaattaat ctgatggcac ctgctttagg tgttttcttg atcaaagcat ggtatggtaa
    16021 aggtcaaact gataacatca cgcagaatct ggggtatttt tctttcccag gattagcaga
    16081 tattccggtc attggaccaa ttttctttaa aaatacattc ttacctgctt attgtgctat
    16141 tttgattgct gtcctagctt gggtgatcat cttcaagacg aaattcggct tgcgcctccg
    16201 ttcagttggt gaaaatcctc aagctgccga cacattaggt atcaatgtat atgctatgcg
    16261 ttattcaggc gtactgattt ctggcttgtt aggcggtatg ggcggtgctg tttttgctca
    16321 gtcgatttcc ggtaactttt cagctggtac gatcgttgga caagggttca tctccatggc
    16381 tgctatgatc tttggtaagt ggaatccttt aggggcaatg ggtgcctcat tgttctttgg
    16441 ttttgctcaa agcttgagta tcattggcgc acaattacca gttatttctt cgattccgcc
    16501 agtattgctg caaattgctc catatttgct gacaatcatt gtgctggttg ttttcttagg
    16561 gaaagcttct ggtccaaaag caaacgggaa gaactatatc aaatcaaaat aatcttttag
    16621 cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc
    16681 aataaaaata aaaagaaaga aggaataatc atgtttaaac gcgtacatct gatcgtcatg
    16741 gattcagtag gaattggaga agcaccagat gccgataagt tcggtgatgt tggttctgat
    16801 acattaggac acattgcgaa agaagcagga ttgaacgttc cacatttaga gcagcttggt
    16861 ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga aagatcatca aggctatgcg
    16921 acgaaattag aagaagtttc tgtagggaaa gatacgatga caggccactg ggaaatcatg
    16981 ggattgaata tcaaaactcc atttcgtgtg tttcctaatg gattccctga tgatctgatc
    17041 aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta acaaacctta tagcggaaca
    17101 gctgttattg acgattacgg tgaacaccaa atgaaaacag gcgatttaat tgtatatact
    17161 tctgccgatc ctgtccttca gatcgcggca catgaagaaa tcattccttt agaagaatta
    17221 tatcggattt gccaattcgt tcgtgatata acaaaagatg atccgtatat gattggccga
    17281 atcattgcac gtccgtacgt gggtgaacca ggagacttca cacgaacaag caatcgccat
    17341 gattatgcgt tgaatccatt cggacacaca gtccttgatt ctttgaaaga agcaggaaag
    17401 gacgtgattg ctgttggaaa gatcaacgat attttcaacg gacaaggaat cactgaatct
    17461 gtccgcacga aaagcaatat ggatggcgtt gaccagctgc tgaaagttat gaaaaacgat
    17521 ttttcaggaa tcagctttac caatttagta gattttgatg cattatacgg tcatcgtcgg
    17581 gatgtagtcg gctatgcgca tgcgattgaa gaatttgact tacgaattcc agaattgctt
    17641 gaagcgatgg aagaagacga cttgctaatg attacagccg atcacggaaa cgatccgacg
    17701 tttactggta ccgatcatac gcgagaatat gtaccgttat tggtttacag taaaaaaatg
    17761 aaagaacaag gggcattgcc tcaaggatat tattcagata tttcagcaac gattgctgag
    17821 aactttagtg tttcttcgac tgaaaatggc gaaagcttct tgaaattatt aaaataatct
    17881 ttttaaatag gagaaagcaa atgaccaagt taagcgagat gttacaagaa acgacccagt
    17941 ttatccttga taaaggagta aaagaaattg attttggttt gattctaggt tcaggattag
    18001 gtgaattagc agatgaaatc gaagatgctg taatgatttc ttatgatgag atcccgttct
    18061 ttcctgtttc gacagtagtt gggcatgcag gaaagctagt ttatgggtct ctggcaggca
    18121 agaaagtgtt agccatgcaa gggagattcc actactatga aggtcattcc atgcaaacag
    18181 tgacctatcc agtacgtgtg atggccggac taggggccca ttcagtcatt gtgactaatg
    18241 catgtggtgg tgtcaacgaa tcatttgtac caggagattt gatgctgatt accgatcata
    18301 tcaattttac tggtgaaaat cctttgatcg gtccgaatga agatgcaatg ggccctagat
    18361 ttccagatat gagtcaagct tatacgcctg actatcaagc aacagcaaaa gcagcagcag
    18421 aaaaactagg tttggatctt aaagaaggcg tatatatggg ctattctgga ccaacttatg
    18481 aaacgcctgc ggagatccga atgtctcgga caataggagc cgatgcagta gggatgtcga
    18541 ctgttccaga agtcatcgta gcagctcaca gcggattaaa agtgttagga atctcttgta
    18601 ttaccaactt agcagcaggt atgcaagcga atctgagcca tgaagaagtt gtagaaacaa
    18661 ctcagagagt caaacaatct tttaaagcat tggttaaaga aacattgaca ttattgtaat
    18721 aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat cgccgataag
    18781 attttacttc cgggagatcc tttgcgagct aaatatattg cggagacttt tttggaagat
    18841 ccagtttgtt acaatcaggt acgcggtatg ttaggcttta ccggcaaata taaaggagaa
    18901 cgtatttctg ttcaaggaac aggaatggga atgccatcag ccaccatcta tgcccatgaa
    18961 ttgatccagt cgtatggtgt aaagaaattg attcgagtag gtacatgcgg tgcattgtca
    19021 aaagacgtcc atgttcgtga tttagtacta gcacaaggtg cagcgaccag ttcttcgatg
    19081 atcgaaaaaa atttccaagc ctttcatttt cctccaatca gtgatttcaa tctattactg
    19141 aaagcatatg agatagctaa agaaaagggc tatacggttc atgtaggaaa tgttctttcg
    19201 gaagattctt tctacaaaga tgatttgaca gagacgttcc aattagctga acttggcgtt
    19261 ttaggagtgg aaatggaagc agcggctctt tattacttag gcgcgaaata ccatgtgcag
    19321 acattaagtt tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac aacagctgca
    19381 gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc gatagcagaa
    19441 taagaaggaa atgaaaaagc tgccctgcat taagtaataa gaacatccaa gcaaaaatag
    19501 ctcctcatgt ttttgacttg gctggactta ttattgcaaa tgtaagcttt tgaacaccgt
    19561 ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa
    19621 cagcttttca tgtttgatga atattgtgac ttgtgaccga g
//
LOCUS       DAPCMT010000042        19431 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPCMT010000042 DAPCMT010000000
VERSION     DAPCMT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.042"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(407..766)
                     /locus_tag="RD743_001260"
     CDS             complement(407..766)
                     /locus_tag="RD743_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDU0563085.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="RD743_001261"
     CDS             complement(796..1137)
                     /locus_tag="RD743_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563086.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="RD743_001262"
     CDS             complement(1134..1808)
                     /locus_tag="RD743_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDU0563087.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="RD743_001263"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="RD743_001263"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDU0563088.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="RD743_001264"
     CDS             complement(4322..5512)
                     /locus_tag="RD743_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDU0563089.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="RD743_001265"
     CDS             complement(5533..6060)
                     /locus_tag="RD743_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDU0563090.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="RD743_001266"
     CDS             complement(6107..8896)
                     /locus_tag="RD743_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDU0563091.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="RD743_001267"
     CDS             complement(9043..9243)
                     /locus_tag="RD743_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0563092.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="RD743_001268"
     CDS             complement(9696..10016)
                     /locus_tag="RD743_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563093.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="RD743_001269"
     CDS             complement(10519..11637)
                     /locus_tag="RD743_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563094.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="RD743_001270"
     CDS             12108..13415
                     /locus_tag="RD743_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDU0563095.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="RD743_001271"
     CDS             13536..14735
                     /locus_tag="RD743_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDU0563096.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="RD743_001272"
     CDS             complement(14762..16030)
                     /locus_tag="RD743_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDU0563097.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="RD743_001273"
     CDS             complement(16200..16841)
                     /locus_tag="RD743_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDU0563098.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="RD743_001274"
     CDS             complement(17250..17753)
                     /locus_tag="RD743_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDU0563099.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="RD743_001275"
     CDS             complement(17876..18640)
                     /locus_tag="RD743_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDU0563100.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="RD743_001276"
     CDS             18748..19023
                     /locus_tag="RD743_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDU0563101.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         5573 a         4137 c         3453 g         6268 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt a
//
LOCUS       DAPCMT010000043        19169 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPCMT010000043 DAPCMT010000000
VERSION     DAPCMT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.043"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(97..822)
                     /locus_tag="RD743_001277"
     CDS             complement(97..822)
                     /locus_tag="RD743_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDU0563102.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(1183..1253)
                     /locus_tag="RD743_001278"
     tRNA            complement(1183..1253)
                     /locus_tag="RD743_001278"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1219..1221),aa:Gly,seq:ccc)
     gene            1370..2122
                     /locus_tag="RD743_001279"
     CDS             1370..2122
                     /locus_tag="RD743_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDU0563103.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            2138..2617
                     /locus_tag="RD743_001280"
     CDS             2138..2617
                     /locus_tag="RD743_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDU0563104.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(2868..4448)
                     /locus_tag="RD743_001281"
     CDS             complement(2868..4448)
                     /locus_tag="RD743_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDU0563105.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(4441..5184)
                     /locus_tag="RD743_001282"
     CDS             complement(4441..5184)
                     /locus_tag="RD743_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0563106.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(5360..6136)
                     /locus_tag="RD743_001283"
     CDS             complement(5360..6136)
                     /locus_tag="RD743_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0563107.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(6384..7220)
                     /locus_tag="RD743_001284"
     CDS             complement(6384..7220)
                     /locus_tag="RD743_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDU0563108.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(7231..8160)
                     /locus_tag="RD743_001285"
     CDS             complement(7231..8160)
                     /locus_tag="RD743_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDU0563109.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            8352..8705
                     /locus_tag="RD743_001286"
     CDS             8352..8705
                     /locus_tag="RD743_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDU0563110.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(8779..9435)
                     /locus_tag="RD743_001287"
     CDS             complement(8779..9435)
                     /locus_tag="RD743_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDU0563111.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            9661..11595
                     /locus_tag="RD743_001288"
     CDS             9661..11595
                     /locus_tag="RD743_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDU0563112.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(11681..12904)
                     /locus_tag="RD743_001289"
     CDS             complement(11681..12904)
                     /locus_tag="RD743_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDU0563113.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(12898..14796)
                     /locus_tag="RD743_001290"
     CDS             complement(12898..14796)
                     /locus_tag="RD743_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDU0563114.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(14790..15908)
                     /locus_tag="RD743_001291"
     CDS             complement(14790..15908)
                     /locus_tag="RD743_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDU0563115.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(15991..16824)
                     /locus_tag="RD743_001292"
     CDS             complement(15991..16824)
                     /locus_tag="RD743_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0563116.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(16817..17734)
                     /locus_tag="RD743_001293"
     CDS             complement(16817..17734)
                     /locus_tag="RD743_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0563117.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(17961..19085)
                     /locus_tag="RD743_001294"
     CDS             complement(17961..19085)
                     /locus_tag="RD743_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDU0563118.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         5974 a         3776 c         3200 g         6219 t
ORIGIN      
        1 aagtactctt ttcataaagt atacgctata tttctttgaa tgtatgtttt tacacaattt
       61 tttcataata ttcataaaaa attcgaatag ctgctactaa ttttttgttt ttgttgcttg
      121 tcctgtctca taattgatcg tgtagccatc ttgaacattg tagataaaga cattgaattc
      181 aatttttttg tcttcaatgc tttctgcttc caattgcact cccctggcta ccagttcatt
      241 cccctcaaaa tgaggagtga cacggtagcg tacatgatga tttgtttctt tgatatatgc
      301 cgcaatatca ttttcgtgag caagcatgta tggagcattc aacgaacgtg tccctgtcat
      361 caagttttta atgttgttat tttgaccagt cagctggaaa ccaattaaat ggctgcgatt
      421 atataaccat tgcccgtcac ttaatttctt ttgtttccaa cctgtcgggt caatatacaa
      481 aggctctctc ttttcagtag ggaacaaatc ttttccaagc atagcattgg cttgtcctac
      541 tctgttcaat ttatcaatat ttgaatagct ttcccatgtt cctttggaaa ggctaagatc
      601 ctcttttgta aattccggct gattcccatt gatttctatt tcttgatgtt ttccatcata
      661 ttctggcaca tccgctactt gagaagcttg tggtgtggaa tcaaaaatag ttgtaatctg
      721 ctgaaagata tctgaatctt tttgtgtggt ggaaacaccg aatgcccctg caatcacagc
      781 agcgacgatc cccacgatcg ccttggttgt ttttttgctc atttctttac tcctttacta
      841 acagctccct aaatcttaac atatgttccc ctataaataa atactagaaa gaaatagaaa
      901 cagcaaaaaa taaaacctgt tttaagatgg cttaaaaata caaaaaagag acggattcct
      961 cgtctctaat cagtcaccac tctatctgta aggtatttat tattattcaa aaattttctt
     1021 catactcaag acaatacact gatttcacaa ccgcttggta aacggtgcgc aaaagcttgc
     1081 atctgcggta ataagtccat ccaagttaaa aacataagaa gttgtttttg cttggctgtt
     1141 cttattactt gttgcagagc agctttttca caacctattt taagcgggtg acgggaatcg
     1201 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcaatacaa
     1261 aaacagtata cgcctctttt attccttcct caagtttttt tattctttac gcttctggta
     1321 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     1381 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     1441 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     1501 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     1561 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     1621 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     1681 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     1741 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     1801 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     1861 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     1921 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     1981 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     2041 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     2101 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     2161 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     2221 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     2281 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     2341 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     2401 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     2461 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     2521 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     2581 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     2641 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     2701 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     2761 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     2821 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     2881 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     2941 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     3001 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     3061 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     3121 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     3181 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     3241 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     3301 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     3361 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     3421 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     3481 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     3541 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     3601 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     3661 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     3721 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     3781 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     3841 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
     3901 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
     3961 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
     4021 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
     4081 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
     4141 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
     4201 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
     4261 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
     4321 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
     4381 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
     4441 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
     4501 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
     4561 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
     4621 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
     4681 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
     4741 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
     4801 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
     4861 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
     4921 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
     4981 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
     5041 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
     5101 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
     5161 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
     5221 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
     5281 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
     5341 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
     5401 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
     5461 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
     5521 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
     5581 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
     5641 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
     5701 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
     5761 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
     5821 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
     5881 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
     5941 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
     6001 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
     6061 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
     6121 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
     6181 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
     6241 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
     6301 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
     6361 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
     6421 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
     6481 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
     6541 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
     6601 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
     6661 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
     6721 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
     6781 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
     6841 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
     6901 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
     6961 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
     7021 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
     7081 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
     7141 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
     7201 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
     7261 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
     7321 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
     7381 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
     7441 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
     7501 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
     7561 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
     7621 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
     7681 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
     7741 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
     7801 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
     7861 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
     7921 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
     7981 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
     8041 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
     8101 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
     8161 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
     8221 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
     8281 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
     8341 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
     8401 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
     8461 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
     8521 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
     8581 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
     8641 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
     8701 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
     8761 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
     8821 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
     8881 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
     8941 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
     9001 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
     9061 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
     9121 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
     9181 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
     9241 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
     9301 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
     9361 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
     9421 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
     9481 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
     9541 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
     9601 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
     9661 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
     9721 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
     9781 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
     9841 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
     9901 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
     9961 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    10021 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    10081 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    10141 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    10201 caacaccctg acattttgat tctagatgaa ccaacgaacc atttagatat cgagacactt
    10261 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    10321 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    10381 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    10441 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    10501 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    10561 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    10621 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    10681 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    10741 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    10801 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    10861 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    10921 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    10981 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    11041 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    11101 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    11161 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    11221 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    11281 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    11341 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    11401 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    11461 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    11521 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    11581 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    11641 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    11701 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    11761 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    11821 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    11881 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    11941 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    12001 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    12061 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    12121 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    12181 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    12241 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    12301 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    12361 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    12421 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    12481 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    12541 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    12601 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    12661 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    12721 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    12781 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    12841 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    12901 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    12961 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    13021 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    13081 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    13141 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    13201 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    13261 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    13321 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    13381 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    13441 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    13501 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    13561 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    13621 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    13681 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    13741 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    13801 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    13861 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    13921 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    13981 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    14041 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    14101 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    14161 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    14221 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    14281 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    14341 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    14401 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    14461 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    14521 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    14581 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    14641 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    14701 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    14761 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    14821 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    14881 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    14941 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    15001 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    15061 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    15121 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    15181 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    15241 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    15301 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    15361 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    15421 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    15481 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    15541 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    15601 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    15661 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    15721 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    15781 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    15841 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    15901 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    15961 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    16021 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    16081 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    16141 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    16201 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    16261 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    16321 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    16381 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    16441 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    16501 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    16561 actggctgta gctaaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    16621 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    16681 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    16741 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    16801 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    16861 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    16921 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    16981 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    17041 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    17101 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    17161 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    17221 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    17281 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    17341 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    17401 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    17461 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    17521 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    17581 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    17641 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    17701 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    17761 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    17821 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    17881 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    17941 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    18001 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    18061 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    18121 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    18181 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    18241 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    18301 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    18361 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    18421 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    18481 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    18541 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    18601 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    18661 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    18721 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    18781 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    18841 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    18901 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    18961 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    19021 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    19081 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    19141 ttaacggaat tagtaacaca aatatttat
//
LOCUS       DAPCMT010000044        18960 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPCMT010000044 DAPCMT010000000
VERSION     DAPCMT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.044"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(154..318)
                     /locus_tag="RD743_001295"
     CDS             complement(154..318)
                     /locus_tag="RD743_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563119.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            613..1941
                     /locus_tag="RD743_001296"
     CDS             613..1941
                     /locus_tag="RD743_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDU0563120.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2143..2976
                     /locus_tag="RD743_001297"
     CDS             2143..2976
                     /locus_tag="RD743_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDU0563121.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2992..3729
                     /locus_tag="RD743_001298"
     CDS             2992..3729
                     /locus_tag="RD743_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDU0563122.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3884..4810)
                     /locus_tag="RD743_001299"
     CDS             complement(3884..4810)
                     /locus_tag="RD743_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDU0563123.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5085..5918
                     /locus_tag="RD743_001300"
     CDS             5085..5918
                     /locus_tag="RD743_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDU0563124.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5952..6791
                     /locus_tag="RD743_001301"
     CDS             5952..6791
                     /locus_tag="RD743_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0563125.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6817..7377
                     /locus_tag="RD743_001302"
     CDS             6817..7377
                     /locus_tag="RD743_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDU0563126.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7576..9297
                     /locus_tag="RD743_001303"
     CDS             7576..9297
                     /locus_tag="RD743_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDU0563127.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9462..10604
                     /locus_tag="RD743_001304"
     CDS             9462..10604
                     /locus_tag="RD743_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDU0563128.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10620..11831
                     /gene="thiI"
                     /locus_tag="RD743_001305"
     CDS             10620..11831
                     /gene="thiI"
                     /locus_tag="RD743_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDU0563129.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12404..13051
                     /locus_tag="RD743_001306"
     CDS             12404..13051
                     /locus_tag="RD743_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDU0563130.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13180..13396
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13444..16089
                     /locus_tag="RD743_001307"
     CDS             13444..16089
                     /locus_tag="RD743_001307"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDU0563131.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16399..17712
                     /locus_tag="RD743_001308"
     CDS             16399..17712
                     /locus_tag="RD743_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDU0563132.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17702..18355
                     /locus_tag="RD743_001309"
     CDS             17702..18355
                     /locus_tag="RD743_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDU0563133.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18410..>18960
                     /gene="radC"
                     /locus_tag="RD743_001310"
     CDS             18410..>18960
                     /gene="radC"
                     /locus_tag="RD743_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDU0563134.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNV"
BASE COUNT         6376 a         3207 c         4009 g         5368 t
ORIGIN      
        1 agtcctactg ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa tcacttgggt
       61 ttctttttct tttattatta tgatgaatca agctttctgt ttttgtcacg tatgttgtaa
      121 tctttacgaa agagtagagt tggctactca taatcaacca tacttcccat catcgctaaa
      181 tacgaaataa atcagcattc ctacaccaat caaagcacaa gctgcaccaa gaaaagggat
      241 gacattccca aagttatctg tattcataag tatgccacca cccacaaaca aaaagccgcc
      301 agcaatcagg tttttcattt tattcatctc ctttcttacg ttatagtatt tccttctttc
      361 aatgagtatt ttgaaagcta tcttattgta tgatgttctt ctaggcctct tctttattat
      421 acaggccatt ttcgttatct catagaaata ttccttttta tctgaaaact attgtatacc
      481 gtatgacatt tttcttcatg aagttgtaag tatattctca ttcaaatgaa aatttcatca
      541 tagaagagat tgtatttcta aattcttaag agtaaaatac gaagtataac ttggatttgg
      601 aaggatgatt ggatgaaagc tattacgcct gatatgacaa caaaatttaa caaacagttt
      661 caagcaaata tcaaacagca ggcattgcag cgggcagttg tgaagaacgg aattactgct
      721 tctgctgaaa atgtttcagc acatgtggag aatacaccgg tattttctat agatttggct
      781 acggggaaag tggctaatca aatgcaatcg gggcgttgct ggatgtttgc tgcactgaac
      841 acattccgtc ataagatgct gaatcaattc catttaaaag agtttgaatt atcacaaaat
      901 tacacttttt tctgggataa atatgaaaag gcaaattatt tctatgaaaa catcattgca
      961 acaggaaaag aggcaatcga tagccggaaa gtagctttct tactcgctac tcctcaacaa
     1021 gatggcggcc aatgggatat gatcgtttca ttattccaaa aatatggcgt tgtgcctaaa
     1081 tcggtgatgc cggaaagtgc gaatagttca aattctcgag atttgaataa ctacttgaac
     1141 aaattactgc gtaaagatgc agtagtgtta cggaaaatgg ttgctcaagg agaaacatta
     1201 gacgctattg aagaaaagaa agaagaaatg ctagaaagca tttataactt cttagctatc
     1261 tcattaggaa cacctccaaa agaattcgat tttgagtacc gcgatgaaga aaagaactat
     1321 catcttgacc gaggactgac accacaaatt ttttatgata aatacatcgg tgtaaaatta
     1381 gatgattatg ttagcgtgat caatgcacca acaaaagaca aaccattcaa tcgttcttat
     1441 acagtagaga tgctaggaaa tgtcgttggc gggaaagaag tcaaatatct gaatgttgat
     1501 atggaaactt tcaaaaaatt agcgattgct cagcttgaag aaggagaatc tgtttggttt
     1561 ggttgtgatg taggtcaatc ttctactaga actagtggca tcatggcctt agatgcttat
     1621 tcaatggatg atctatttga tacggatttt acaatgacta aagctgaacg tttagatttt
     1681 ggagaaagtc tgatgaccca tgcgatggta ttgaccggtg ttgacttagt agacgggcaa
     1741 tcaacaaaat ggaaagtcga aaatagctgg ggcgaaaaag ttggaaagaa tggcttcttt
     1801 gttatgagtg atgcctggat ggacgaatat acgtaccaaa tcgttgtaag aaaagattta
     1861 ttgacgaaag agcaattaaa tgcgttcaat gaagaaccaa ttgttctatc tccgtgggat
     1921 ccaatgggtg ctttagcata gacgagtatt tcaatgaaga gtatgtagta aagataaaaa
     1981 gagctggctt aagccagctc tttttatctt taaagtccat tccctgtttt ttatggtccc
     2041 tgtgctataa ttctaaataa aggataagga ttggaaataa tccttttatc attaaaaata
     2101 tgatgacgaa gtagaataaa attgggagta ggggggaaag tcatggatat ccaaactgtt
     2161 ttatctgatc tgaaattaaa tggtaaagtg attccagttg ttggtggcga tgttaatcag
     2221 acctatcgta taaaaaccga acatcgtgct tattttttaa aaatccatcc gaatgtaaaa
     2281 aaaggatttt ttgaagcaga agtagacgga ttaaaagaac tgtcagcatt cgtacgtgta
     2341 ccggatacgt atatgctagg tgaaacaagt gaaggcgctt atttgttaat ggaatggatc
     2401 gagccgggaa aaggggatca acgagatctt gctgcagctt tagctaatct ccatcagcaa
     2461 acagcgccac aatttggttt tcgtaaagat aactatttgg gtacacttgt tcagaaaaat
     2521 tcttttgaag aagattggtg gacttttttc tttaaagatc ggcttgaatc ccagatttct
     2581 ttggcagaag aaacgaatcg atggaatgta cagcgtcaag aaaagtatct gcgatttaaa
     2641 gaacgtgtat tgaaatcggt tgaaccaaaa aagatcactc cacgcctctt gcacggagac
     2701 ctgtggagtg gtaatgtgtt ctttgaccag caagggcatc cagtttttgt cgacccagct
     2761 gtttcttatg ggaatcggga acaagatatt gcaatgagcc agttattcgg tggtttccga
     2821 ccggaatttt tagatgctta tcaaactata tttccattag aaaagggatg gaaagaccga
     2881 ctgccgattt accagttata ttatcttttg gcacatttga atatgtttgg agaaagttac
     2941 ggttcccaag tagatcaatt actagaaaat ttttaagagg agggcgtgtt tttggcaaaa
     3001 aaatatacca aaaagcatga agtttcatat tatgagtgtg acatcaacca aacgatgaca
     3061 tttccttcga tgttaggagt agttatcaaa acgtctgaag atcaaagcga ccagttggga
     3121 agaggcagtg aattcgtcaa tagctttgga cttacttggg tcatcacgaa ttattccatg
     3181 gagattaatc gtctccctag agtcggtgaa gtaatctcag taacgactca agccatggag
     3241 tacaataagt ttttctgtta tcggaacttc tggatccatg atgaagatgg gaaagaatta
     3301 gtcaaaatag aatctgtttt cgtattgatg gattttgtga atcgaaaaat gagcagtgtt
     3361 aatgaagaga tcattgctcc ttttgagagt gagaagatca agaaaatcaa gcgtcaggaa
     3421 aaaattgaaa agattgattc gggcttcatg ttgccatatc gtgttcgttt ttatgatatc
     3481 gatagtaatc agcatgttaa taatgcgatg tattttaatt ggatcattga cgtattagga
     3541 tatgactttt tgactacgca tgttccagaa aaagtaatta ttcgatttga taaagaagta
     3601 gagtacggga atgagatcga aagccatttt gaacaaatcc ctatcgaaaa tggtgtcaaa
     3661 acaaaacatg aaatccgaat gcaagaacag ctgtattgtg aagcaaatat tatatggaaa
     3721 aataaataaa aaagaggttg tgacagaagg gggcaacttc aagaaataag aagaaattta
     3781 cgaaaattgt ttctcaaatt tttgtaaatt ttcgacttag ttttcgaaga agctattttt
     3841 gtcacagcct ctctttttaa gtttagctat tagataatga tgtttagttt tggataaacg
     3901 aaatcaattc ttcacccgac aaaccatagt ttacagcaat caatagtttt aaacgtgctt
     3961 tttgactatt aatactgtta cagaaaataa tccccatttt ttctagttct tttcccccgc
     4021 cttcataatc ataaacaggc tctgcgattc cattgaaaca acgagaaaca agtactacag
     4081 ggattttttg gacaagaagc ttttccaaag cagctaaggc ttgtggaggg agattccccg
     4141 cacctaaagc ttcgattacc aaaccgtcta ctttcgtatg ggcgatggct tccaataagt
     4201 ccccttgcat cccagcatat gctttaacga tcgggatagt cccatctaca gcggaaatgt
     4261 ccagtcgctt tgtttctaaa agttcctgca agaataaaat tcgattctta gttactaagc
     4321 cgatgggacc caatgtaggt gttcggaaag tagcaacatt tgtggtatgg gtctttgtca
     4381 cgtagcgtgc agaatggatc tcatcgttca tgaccaccaa aaccccctta tctagcgctt
     4441 cttcacaact tgcgacacga atcgcacttt caaaattata aaggccgtca cttccaagtt
     4501 cattacttga acgcattgcc ccagtcaaaa caattggtaa tttgttgcca atcgttgtat
     4561 caagaaaaaa agcagtttct tctaatgtat ctgtaccgtg tgtgatcacc actccggata
     4621 tacctgatgc ataagcggac aagatacgtt cttttaatac caacatgtga gaaggcatga
     4681 tgtgtggtga gggaagattg aagatatctt caactaataa ttctacatgc ttggggattg
     4741 tgatttctgc atctaataat gggtttgcca catcaggact aacaccgccc gtgagcatgt
     4801 tttctttcat ggcgatcgtt ccgccggtgt gtagtacaag tatttttttt cattttacag
     4861 acttccttac gaataatttt gcatagccgt taaatcttat tgtatactat ataaaagagt
     4921 tttgctatca gtgaagaata acgaatagcc taacttatca taaaaaagct gaccttatta
     4981 aaatggaaaa gactcaacct tgacctccta atacaatgga gtatctggtt gagtttcttt
     5041 tacaagaaag cagccaagga aatcaatcac gaaggtggcc ggaaatgatc agaatcggat
     5101 taacatcgtt taaagaacat gacaaattaa ctggtaaaaa gaataacact ctttatgaat
     5161 atgcagggtt tctaccctta gtggaattag atactgccta ttatggaatt ccccgaaagt
     5221 cctcagttga gaattggata agtgaagtac ctgaatcatt tcgctttgta gtcaaagtct
     5281 atcaaggaat cagcggtcaa ggagactgga agccttatta tcagaccgaa gaagagatga
     5341 ttgcgcattt tttagatgtt atgtcgccaa tggtagatgc tggaaagatg ttttgctttt
     5401 tagtccaatt cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac ttgcgcaaac
     5461 ttagaaagtg gtttgagggt tatcctgttt ctatagaatt gcgggattat tcttggtaca
     5521 gcgatgaatt tcttgaacgt acaagaaact ttatgtctag agccgctttt tctttagcca
     5581 tcatcgatga gccacagttg ctgaatacaa cgattccttt tgatccattt gtcaccaatc
     5641 cgaactttgc tctttttcga ttccatggta ggaatgcgca aggatggcaa gcaaatgata
     5701 aagagtggcg taaaaaaaga acgttatatc gttattcaga aaaagaactg caagaactag
     5761 cacatgaagt caaagtagtt gattctaaga caaaagaggt aggtgtgatt tttaataata
     5821 attctggtgg tgatgcggct gaaaatgcat tagcactgca aaaaatatta aatatcaagc
     5881 cagatggact caatccaacc caaatggatc tgttttaagg gcttatttta ttcaagaaag
     5941 gggcgaagtc tatgatcaaa gctatttttt ttgatattga cggaacattg gtcaccaatc
     6001 gttcaaaagc gttggaatca acaaaacaag caattgaata tgctcggaaa aacggcattt
     6061 tatgcggtgt agcaacgggg cgcagcccag taaagatcaa agaaatcatt gatgaattgg
     6121 aattagatat gtatgtcgtg tataatggtc aactggtttt tactgcagat cgcacgatta
     6181 tcgaccaccc ttttgaacaa aaagtattgg aacatattgt tgaatttgca gatgagaacc
     6241 atcgacaaat cgtttttggt gcgagaaatc ggttagatgg atcaacgacg atgctattag
     6301 gacaatcgat ttttatcaag cgactagtca gctttttacc gcgtaaattc ccggtacgtc
     6361 tgatgaaaaa aatcctccag gtttttagcc ctcaccgaca aaaggatcgt tatgaaacgt
     6421 tagagatatt aaaagaacca atttatcaat gtatattgct cagtccggag tcagaacaga
     6481 aaaagttaga acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg tatactgtag
     6541 atatcatccc aaaaggtggt tcaaaattac taggcatcca agctttcgca aatgctgtcg
     6601 aaattgaaat ggaagaaatt atggcattcg gcgatcatta caatgatatc gagatgctga
     6661 aaggtgtagg aattggagtg gccatgggga atgctcagat cgaagtaaaa catgctgcag
     6721 actttgtgac tcaatcaaat gaagaagatg ggatatacga aggactgaaa cacttcggtc
     6781 tgatttatta gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac accttttcaa
     6841 aaagtagaaa cattccacaa aacttttgat ccaagaagac cagagatacc cacggctttt
     6901 acagaaaatg aagctcatac acgtgctgcg ttcaaaatag aagaacttgt tgaatttgtt
     6961 gcagctactt cacctaataa agaacaactg catgaagctg ttatgctgct gcacgaagca
     7021 ttagataaag cagaagaaaa aatacttggt aaaacacagt ggggaagtga tccgctggtt
     7081 gaacaagtag atgcactgac agatatcctt tactttactt atggttcttt ttcattacta
     7141 ggaatagtgc ctgatcgaat attcgaaatc gtccatcagg caaatatggg gaaattattt
     7201 ccagatggaa aacctcatta tgaccctgta acaaataaag tgttgaaacc tgaaaactgg
     7261 cagatggaat atgctccaga acctaaaatc gcggcagaaa tccaacgtca gattcaagaa
     7321 gcgattgcta agaagaaatc cgctgatata ggcatgtgca caaatgaaaa agagtgatca
     7381 gagtttcact atttttgtta tctttatacg atggaaaaac aaaagatttt cgtttaagtt
     7441 agtgaatgca tgaaaatagt atgctataat ctagaataga tgtcttttgc acgagagacg
     7501 tattatttgg atttatgcca agcagaacgc catctgttgc ataaaagatg catactgaaa
     7561 acggaggaac cataaatggg aaataatgtt atcatcggaa tcgtcattgc aatcattata
     7621 gtcgccattg tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca agatcgattg
     7681 gatgccttag aaaaacgcaa ggaagaatta tttgacttgc cagtgattga agaagtcgat
     7741 gatgttaaaa aaatgcatct ggttgggcaa agccaaaata cctttcgtga atggagtcaa
     7801 aaatggaatg acatatctac ttcctcgttc gctgaactag aaagtcaaat tttcgaagtg
     7861 gaaaacctca atgaaagttt tcggttctta aaaggaaaaa aagcagttgt tgaagcagaa
     7921 gctacaatgg accaaatgga gaaacaagtg acggaaatcc gtgaaggtct aaaggaattg
     7981 cgggaaacgg aagaacgcaa ctcactagct gttcagcagg cacttgacgt ctatgaagaa
     8041 ctgaaagaaa aagtcagaga agacgagcat gaatacggac cagctctttt tgaattgcaa
     8101 aaacagatca aagtgatcga aaaagaattt acgcagtttg tgactttaaa cacttctgga
     8161 gaccctgttg aagcccgcga ggttttggat aaagcagaaa aacatacgta tgaagtggaa
     8221 gctctgatga aaaaaattcc accgctgtat gaggagctcc ataccacttt ccctgaacaa
     8281 cttgaagaaa tcagtgatac ttacgagaaa ttgatggaag aacaatatgt attcccagaa
     8341 gaagatcttg ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc attagccaat
     8401 cttgaaaaaa cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc tgatttgatt
     8461 gattcgttat atgatatctt agaacgggaa atggaagctc agagatatgt aaaaactaac
     8521 caatcaacga ttgcagagta tatcaaacat acaacaaaga ataaccgtca attattgatt
     8581 gagctggatc acacatctca aagctataca ttgaaccaca atgaaattgg ccgtgtacga
     8641 ggtttccaaa ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat tccacagatc
     8701 cgtcaacacg aaattcctta ttcagaagtg cgcacgttct ataaaacagt cttcaaagtc
     8761 ttagaagata tcgaaaagca acaagtagag atcgacgact cattacatga attgagaaaa
     8821 ggcgaaaaag aagcgcaaga aaagatcgat acattcgaat tcaaattgcg cagtttgaaa
     8881 cgttttgtag aaaaacaacg tttgcctggc ttgccgaatg attacctaga attctttttt
     8941 gttgctactg accgtattga agaactaagt gttgtcttga ataaaattcg agtaaacatg
     9001 gaagaggtca atcgtttagt tgctctatgt gaagaagatc tagaattatt agataagaag
     9061 acacatgatt tagtagatgc agcagcatta acggagcaaa tgctccaata tgcaaatcgt
     9121 tatcgtcata cacacgaaga agtgagagaa gcaattgata aaagctacta tttattcaat
     9181 aaagaatatc actatcaaga agcattagac gaaatcggta cagcattaga gcgagtagaa
     9241 cctggtgctt tcaaacgcat cgaagatttt tattttaatc atccagactt agtataaagc
     9301 aaaaaagtta gtaaaccagc aagagcaaga cggattattg tcttgttctt gtcttttttt
     9361 gtatatttga aaattgaaac aagtgaaaat ttcacctgct cttgaaagca ccttggacaa
     9421 ccgttataat agaaaagaat ttcaaaaaag aggggtttct tatgatatat tttgataaca
     9481 gtgcaacaac cccaatcttt ccgcaaagct tagatgcata tgtaaaaacc agtcagcgga
     9541 ttattgggaa tccatcaagc ttgcatgatt taggcaatca agctaaccgg ttgttgcagc
     9601 aagcaagaaa gcagatcgct gatttgatcc atgtagaacc agaagaaatc tattttactt
     9661 caggcggaac agaaggagat aactgggtat taaaaggaac gatgatcgaa aaaagagaat
     9721 atgggaatca tatgatcatt tcaggagtcg aacatccggc cgtcagcgag acagcagaac
     9781 aattaaaaga attaggaatc gaagtaagta tcgccccagt ggataaacgc ggatttgtac
     9841 gtgtcgacga attgaaagaa ttaataagaa aagagacagt attagtctct gttatggctg
     9901 tgaataatga agtaggatcg attcagccca tccaggaaat cagtgatctt ttggatgatt
     9961 ttcctaaaat tcatttccat gtagatgctg ttcaggcgat tggaaaagta ccattctcgc
    10021 aatggttgac agaaagagtt gattttgcaa cattttcagc tcataaattc catggcccac
    10081 ggggaactgg tttcatttat tggaaaaagg gtcgtcgttt agcaccatta ctgacaggtg
    10141 gagggcaaga aagaaatcag cgaagtggga cagaaaatgt tccaggtatt gtggctatgg
    10201 ttaaagcttt acgtttatca ttagagataa aggaacaaaa acctgagcag acacaacgct
    10261 taaaagtagc tctaatcaat gcgttgaaga cgtatgagaa agtgacgatt ttttcagaag
    10321 gaactgacta tgcaccgcat attttatgtt ttgctctaaa aggaattcgc ggcgaagttc
    10381 tcgtccatgc cttagaagaa aaacaaatat ttgtttctac tactagtgcc tgctcctcac
    10441 gtaaacatat ggcaagcagt acattgcacg caatgcatgt accaaatgat ctggctacat
    10501 ctgctgttcg aatcagctta gatgaaaaca attcgcttgc agaagtcgag caatttatga
    10561 ttattttcaa gcagctttat aagaaatttt ctgcaatcaa ttaaaagagg agtgttcttg
    10621 tgaaatacag tgaaattatg gtccgttatg gggaactttc gacaaaagga aagaatcgaa
    10681 aaagctttat catgcaacta gcacaaaatg tcagacaagc tttgaaagac tttccagaaa
    10741 tcaaaatcca tgctgatcgg gaccggatgc atcttttatt gaatggcgca gacgggcagc
    10801 tagttattcc aaaactagca aaaatatttg ggatccaaaa tttctctcca agtattcgtg
    10861 tagaaaaaga tgtcgaagtt ttgaaacaag ctgttcaaga catcatgaaa gaaatttata
    10921 ctgggaaaga aacatttaaa attatcgcta aaagaagcga tcaccaattt gaattgacaa
    10981 gtaatgaatt gaatcaaaca ttaggtaatg ctgtttttga tattttcccg catatccaag
    11041 ttcaaatgcg tcaaccagat attccattac gtgtagagat tcggcgtgat ggcgcttatc
    11101 tatcgtatga aacgatcaag ggagctggag gattgcccgt aggaacaagc ggaaaaggaa
    11161 tgcttatgct ttctgggggg attgattctc ctgtagcagg gtactttgcc atgaagcggg
    11221 gagtggaaat tgaagctgtt cattttgcta gtccgccgta tacaagtgaa caagcactgc
    11281 aaaaagcaaa agatctaaca gccaaactag caccttatgt gggaacgatc cattttatcg
    11341 aagtgccatt taccgagatt caagaagaaa tcaaaaagtc agtgccacaa ggatattgga
    11401 tgacaattac tcgtcgaatg atgttgcgtt tgaccgatga aattcgccgt attcgtcatg
    11461 gtttagtcat tttgaatggt gaatcattag gtcaagtggc ttcacaaact ttgcaaagta
    11521 tggtggctat caatgaagta acgaatacgc caatcattcg gcctgtagcg acaatggata
    11581 aacttgaaat cattgaagta gcagaacaga tcgatacatt taatctttct atccagcctt
    11641 ttgaagattg ctgtacgatt tttgctcctc cacagccaaa aacacgccct caactggaaa
    11701 aggtccatca atatgaagag cgtttagcaa tagatgaaat gatccaacga gcactagctg
    11761 gcttgaagat ggaagagata caagcagaat cgtctaatga aaaaactgaa gaattttcag
    11821 aattgctata agaggttatg aaaatagtgt tttgaccaga gtattagaga aacaaaacga
    11881 aaaaatagct ccatgtattt taccgttttt gaactaatgt cgaagattag ctgattgaat
    11941 accatttatc aagaagttat ggaaaagccg gttctttttt actaactgaa aaataaaatt
    12001 ttatttagag atcaagctac agttgataaa tgatataaaa aagagaattt cccatgaaat
    12061 agcgaggttg tgacagaagt gggcagcttc aagaaataag aaggaattta cgaaaattgc
    12121 ttttcaaatt tttgtgaaat ttcggcttat ttttcgaaga agttacttct ggaacaccgt
    12181 gtatcagagg ttgcgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat
    12241 agttcctcat attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc
    12301 gttttatttg tctcacaccc ttttccttcc tacttgtaac cttgctcccg tgttagaaac
    12361 atgttacact aaatatgtga aattattaac aaggagcgtc gttatgcgag aaaaaacaga
    12421 tacactgcca aaagcaactg cgaaaagatt acctttatat ttacgctact tgaagatgct
    12481 ggatgactca ggtataagta gaatcaaatc aaatgagttc agcgaaatca cccaaattcc
    12541 ttctgcaacg atccgcagag acttctcgca attaggcgag ctaggcagaa gcggatatgg
    12601 ctatgatgtc ccttttctta tcgatgtatt caataatatc cttaatacta aagaagaaaa
    12661 acgaatcgca ttagtaggat atggaaatct tggcaaagca ttgaaacaca acaattttag
    12721 aagaaatgaa aatctaaata tcgtgtgcgt ctttgataat gatccagcac tgatcaatcg
    12781 agtaattgat ggagaaatga tctatccgat tgatcgtttc gcggagatag ccaaagcaaa
    12841 aaatgtgaca gtagcaatct ctacggtacc aagtaaatat tcacaatctg ctattgatga
    12901 aattgtcaaa ggaaatgtaa ccgctatact taattttgcg cctgacagag taactgtccc
    12961 agcttatgtc aatgttcaat acattgattt gacaaccgag ttgcagacgc ttatttactt
    13021 tgatgagaat tactcagaag ttttttctta ggaaaatttg agtaggctta ttgatcttgc
    13081 gagaactcgt cgtaattctt tcaaaaattt tttagtacta aatgaagtgg ttttttcacg
    13141 accacttgtt gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct gaacaagagg
    13201 agtaactggc acattcattc ttagagagag aactatctgc tgaaaggttc tcaatgacaa
    13261 ccagtgaagg tagcttggga gtttctgtct tgaatggagt agagatagac gagtcgtgat
    13321 cgttaactca cttgagaggc aataatgatt gattattgca atttaggtgg taccgcgtaa
    13381 atcatacgtc ctagaatacg aaagtatttt aggacgtttt ttagtttaaa ggaggaaaac
    13441 agcatgacgg aagaaaataa cctatcaaca aaatataatc cgcaagaagt tgaagctgga
    13501 cgttatcagc attggttaga acaagatttg tttaaaccga gcggagataa aaaagcaaaa
    13561 ccttattcaa tcgtaatccc gcccccaaat gttactggaa aacttcattt agggcatgct
    13621 tgggatacga ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg atatgatact
    13681 ttatggctgc ctggtatgga ccatgcagga atcgcgacac aagcaaaagt agaagcgaag
    13741 cttagagagc aagggatttc tcgttatgat ctaggtcgtg aaaagttcat cgatcaagtg
    13801 tgggaatgga aagaagagta tgcgagccac atccgcgaac agtgggctaa aatgggctta
    13861 tctttagatt acagtaggga acgttttaca ttagatgatg gattatctga agcagtacgt
    13921 aaagtttttg ttaccttata cgaaaaaggt ttgatttatc gtggtgaata tatcatcaac
    13981 tgggatcctc aagcacgaac agctctatct gatatcgaag ttattcataa agatatcgaa
    14041 ggcgcctttt atcatatgag ctatgaacta gcagatggat caggcgtagt agaaattgcg
    14101 acaacccgtc cagaaacgat gttgggagat acggcgattg ctgttcatcc agaagacgag
    14161 cgttatcaag cgctcatcgg caaaaaagtg attctacctt tagtcaataa acaaatccca
    14221 atcattgcag atgaatatgt agatatggaa ttcgggacag gtgtagtaaa aatcactcca
    14281 gcacatgatc caaacgactt tgaggtaggt aatcgtcatg atcttccacg cgtaaatgtt
    14341 atggatgatg atggtacgat gaatgaacta gcaggaaaat atgctggaat ggaacgtttt
    14401 gctgctcgta aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat taaaatcgat
    14461 acgatgatcc acagtgtggg tcattccgag cggacaggtg tcgttgttga acctcggctg
    14521 tctactcaat ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga taaccaagaa
    14581 acagatcaag cagtggaatt cttccctcca agattcaatc aaacgttttt acgttggatg
    14641 gaaaatgtcc atgattgggt aatctcacga caattatggt ggggacatca aattcctgca
    14701 tggtatcaca aagaaacagg tgaaatgtat gtagggatgg aagcaccagc ggatagtgaa
    14761 aactgggtac aagatcctga tgttttagac acttggttta gttcagcttt gtggcctttc
    14821 tcaacaatgg gctggccaga tacggaaagc gaagattata agcgatactt ccctactagc
    14881 actttagtaa ccggttatga catcattttc ttctgggtaa gcagaatgat tttccaaagc
    14941 ttggaattta ctggcgaacg tccatttgag aatgtcttga ttcatggatt gattcgtgac
    15001 gaacaagggc ggaagatgag taaatcatta ggtaatggaa tcgatccgat ggaagtaatc
    15061 gaaaagtatg gagcagatgc tctccgctgg ttcttatcga atggttctgc accaggacaa
    15121 gatgtccgct tcagctatga aaaaatggat gcttcttgga actttatcaa caaaatctgg
    15181 aatgcttcac gtttcgttat catgaatatc gaaggaatga ccgtagaaga gatcgattta
    15241 tctggagaaa aatcagtggc tgaccggtgg atccttactc gtttgaatga aacgatcgaa
    15301 cgagtaactg agctatttga tcgttttgaa tttggtgaag caggtcgaca actgtataac
    15361 tttatctggg atgatttttg tgactggtac atcgaaatga gtaaagaagt actttatgga
    15421 gatgatgctg tttcaaaaca aaccacccgt agcatcttag tccatacact tgatcagatc
    15481 ctacgtttat tgcatccaat catgccattc gttacagaag aaatatggga acatattcct
    15541 catcagggaa attctctagt agtggcagaa tatcctgttg tacgtccaga gtttgatgat
    15601 gaaacagctt ctaaagggat ggaagtattg aaagaattga ttcgagccgt tcgaaatatt
    15661 cgttcagaag tgaatacacc attgtctaaa ccaattacgt tgctaattaa aacaaacgat
    15721 gctaaaatcg atcaattttt agttgaaaat acaagctata tcgaacgctt ctgtaatccc
    15781 gaagagttga cgatatcaag tgagatcact gcgccagatc tagcgatgtc agctgttctg
    15841 acaggtgcag aaatcttctt gccgttagca ggattgatca atattgaaga agaaatcaaa
    15901 cgattagaaa aagaactgga aaagtggaca agcgaagtaa aacgtgtcca aggaaaactt
    15961 tctaatgaac gttttgtttc taacgcgccg gaagaagtag tagaggcaga gcgtgcaaaa
    16021 gaaaaagatt atttagagaa gcaaacggct gtcaaagaac gaatcgaaag tttgcgtact
    16081 attcaataag aatagaaaaa gagagtgtga aatcagctag ctattactaa aagctagctg
    16141 attgaacatc gttacaggaa agagtttgag cctcactctt agcttcgatt cataaggaga
    16201 tgtgacataa attttgtcgc atctcttttt tagtctactc ttattatgta ggagaaggag
    16261 ggaggtggat catgtatcaa gagtcaataa agtagttgat aaaatcgttc ctctctctgt
    16321 ttcttttctc tttgtcatac ttttattttt cttatgtgtt ctatttgtta taataaaaga
    16381 gaaatggggg aattgttgat gacgattgaa gaagcaatag aatggatcca tagtcgatta
    16441 ccatttggtt cacgtcctgg ccttgaccga gtcgaagctt tattagaagt ggtagggcat
    16501 ccagagagaa aagtaaaaac gatccatatc gctggaacaa acggaaaagg ttctactgta
    16561 gcttatttga gaagcctatt agaagaggct gggctgacag taggtacatt tacttcacct
    16621 tatatcgaaa cgttcaatga acggatcgca atcaatggca aaagcattcc agacaatgat
    16681 ttgatccagc ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca atttgaagca
    16741 gtagctggta ttactgaatt cgagacatta acagcaatgg catttgatta ttttttgatg
    16801 aagaaagtgg acgtagcgat tatcgaagtt ggcttaggcg gcttattgga ttctaccaat
    16861 gtagctgtac caatgctgac gggaataaca acgattggac ttgatcatac cgaaatactt
    16921 ggagatacaa ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca aggcgttcca
    16981 gtagtaacag gaaatattgc cccagaagct tttactgtca tccaacaaac agctaaagca
    17041 aaaaatgcac cgctttatgt atacggggaa aattataaaa taaactatct gcatcctgat
    17101 gagatgtggg gagaagtatt tgaatttttt aatgaaatag gaaagatccc tcaattaacc
    17161 actccgcttt tagggcaaca tcaagctgaa aatgcaggag tagccatcga attgtatttt
    17221 gtttactgtc aattggaaaa gctgcctttc cgagaaaaag atgttttgaa gggattgaag
    17281 aaatctcgat ggccaggaag aatggagcgt ttgagtaacg agccattagt tgttttagat
    17341 ggtgcccata atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa agaattcaaa
    17401 ggatatcaaa tcaacatttt gttttccgcc ttacgaacta aaaatgttag agaaatgcta
    17461 gaacgattgc agcaagtacc aaatgtccat atttatttga cgacctttga acatccaaaa
    17521 gccatcgaat taagtgaagt atcagattta gcaagtgaga gagtatcaat cgtttcattg
    17581 tggcaattcg gattagcaga tatattggaa aagatgacaa atgaagacgt attagttgtg
    17641 accggttcac tttatttcat ttcagaagtt cgccatttat taatacaatt aggaggagat
    17701 catgaagtat aatgcagtga tttttgacat ggatggtttg ctgttcgaca cagagttagt
    17761 gtattacgaa gccacacaag tagtagcaga caagatgggt tttccttatg ataaagacct
    17821 gtatttaaaa ttcttaggtg tttcagatga agaagtatgg gcgaattatc atatgatttt
    17881 ctcggaattt ggcaaaacaa gtgtacaaag attcatagat gattcatacc aggaaacgct
    17941 tgaacgtttt tcgcaaggag cggtacaaat gaagcccggt gtgttggaac tcctctcttt
    18001 tttagaagaa catcagattc ccaaagttgt cgcttccagt aaccaacgag cagtcattga
    18061 acttttattg aagaagaaca atctgtttga acgatttgag acgattgttt ctgcagaaaa
    18121 tgttcaacga gcaaaaccag acccagaaat tttcttgtta gctaatcaga agctaggtac
    18181 agcgaagaaa gaaacattga ttttagaaga ttcacaaaat ggtattttag cagcttctgc
    18241 ggctgagatc ccggtagtca tggtaccaga tctgcttcca ccatcccaag aattgatcaa
    18301 aaaaacagct ggggttgttt cttctttaca tgaaatccct gcattgttaa aataaaaatc
    18361 catctctttt cgtatcttat tttagataag atagaaagga gatgtttttt tgggaaaacg
    18421 attgatcaaa gaagtaccta ctagctcttt accaagggaa cgtatggaaa tttatggcgc
    18481 ggaagcttta tcggatcagg agcttttggc aatcttactg cgtaccggtc aacaccctta
    18541 tagtgtgatg tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg cctctttacg
    18601 acaggctact ttgcatgagt tagaagagat tcgagggata ggacgcgtga aagcaatcga
    18661 aatcaaagcc ttgatcgaat taggtcgacg aattcagacg gatcatgaaa gagcggctcc
    18721 accggttcgt accagctacg aattggccca gcaattgatt ctggaaatga aagatcacaa
    18781 acaagaacat ttggtttgta tttatttgga cacgaagaat caagtgatct taaaaaaaac
    18841 agtgttcatc ggttcgctga atcagtcagt cgctcatcct agagaaattt tccattatgc
    18901 tgtacgttat tgtgcagcaa ggatcgtttt agcgcataat catccttctg gtaatgtttt
//
LOCUS       DAPCMT010000045        18486 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPCMT010000045 DAPCMT010000000
VERSION     DAPCMT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.045"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="RD743_001311"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="RD743_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDU0563135.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="RD743_001312"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="RD743_001312"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDU0563136.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="RD743_001313"
     CDS             complement(2169..2906)
                     /locus_tag="RD743_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDU0563137.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="RD743_001314"
     CDS             complement(2997..3524)
                     /locus_tag="RD743_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563138.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="RD743_001315"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="RD743_001315"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDU0563139.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="RD743_001316"
     CDS             complement(4401..4886)
                     /locus_tag="RD743_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDU0563140.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="RD743_001317"
     CDS             complement(5039..5236)
                     /locus_tag="RD743_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563141.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="RD743_001318"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="RD743_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDU0563142.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="RD743_001319"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="RD743_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDU0563143.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="RD743_001320"
     CDS             9211..9423
                     /locus_tag="RD743_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDU0563144.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="RD743_001321"
     CDS             9425..11104
                     /locus_tag="RD743_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDU0563145.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="RD743_001322"
     CDS             complement(11358..11558)
                     /locus_tag="RD743_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0563146.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11851)
                     /locus_tag="RD743_001323"
                     /pseudo
     CDS             complement(11708..11851)
                     /locus_tag="RD743_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12086..12748)
                     /locus_tag="RD743_001324"
     CDS             complement(12086..12748)
                     /locus_tag="RD743_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDU0563147.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13140..13199
                     /locus_tag="RD743_001325"
     CDS             13140..13199
                     /locus_tag="RD743_001325"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDU0563148.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            13257..13988
                     /gene="erm(A)"
                     /locus_tag="RD743_001326"
     CDS             13257..13988
                     /gene="erm(A)"
                     /locus_tag="RD743_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDU0563149.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(14114..14896)
                     /locus_tag="RD743_001327"
     CDS             complement(14114..14896)
                     /locus_tag="RD743_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDU0563150.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(15050..15427)
                     /locus_tag="RD743_001328"
     CDS             complement(15050..15427)
                     /locus_tag="RD743_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0563151.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            complement(15434..17326)
                     /locus_tag="RD743_001329"
     CDS             complement(15434..17326)
                     /locus_tag="RD743_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0563152.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            complement(17323..18408)
                     /locus_tag="RD743_001330"
     CDS             complement(17323..18408)
                     /locus_tag="RD743_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0563153.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
BASE COUNT         5512 a         3830 c         2965 g         6179 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa tttcatatta tcaagagtaa attacaaaag
    11881 aatcatttaa atcaatactt tcactttgaa tacatgtatt tgaagtggaa ggtacttatt
    11941 tcaaaaatag taaaacctgt atcttaaatt acttaatagt aacataagat acaggctgat
    12001 tttttattca ttgttattta tactaaagca cccgatagct ctgaaaacaa tcacaaattc
    12061 aacttttcaa agccacagct ttaagttatt ttgtccagac aacccccatt gcccaaccca
    12121 ttttatggaa ttggcatcca ggcaacaact tttcatataa atcgtaataa ttttgttcag
    12181 ataggtactt atctgaagct aaatgctcaa gccatgattt agatgtgttg tgattataaa
    12241 ttctaatcgc attttttatt ccaaatttga aacaatgcgg taaaaactct tggattgccc
    12301 ctaatttata aacataggta ggcggtgtct cgacttcaga tacattatca agaataacta
    12361 ttcttccttc ctcattcaac agttccttca tttgctgtat tacgctggct atatcatcca
    12421 aatgatgaaa ggttgttcgg cttacaataa aatcaaactt ctcattaaaa ttaagttgtt
    12481 ctgcattcat attcagatag accgtatttg ttagttgacg tttagatttg gcaagatcga
    12541 gcatttgatt agaaatatca atccctacca cttcatcata ataacttgct aatttctcca
    12601 ctaacaaacc cgagccacat ccgatatcta atgctctgcc tttctttgga gacatattag
    12661 acacaaagaa tgaataatca ttcaaaagct cattcacgaa atcgtaatct tctgcaacct
    12721 tatcaaactg tgattctatt gtattcaaaa agatccccca ttcctacttt atcgacattc
    12781 tttcattact taccacttta gatgtttttt cgttggggat aaaacttccc tttagacaat
    12841 tttatccaaa gacaatacaa cagtgcaact ttattaaagt cactgtcctt tatcgcagcc
    12901 tttacttttt agtaaagaca gtggcttctc ttatcaagtt tcaaaacata ttattttgaa
    12961 gaaaacgtcc atctgaagtg tcaagtgcaa aattacatat aaaggtttat tctaaaatga
    13021 aaagatgata caatcatatt cagttacata aggaggtttc aattatgtgc accagtatcg
    13081 cagtagtaga aattacttta tctcattcat aatgaaaaaa atggaaagga gataaaagta
    13141 tgggtacttt ttctatattt gttattaata aagttcgtta tcaaccaaat caaaattaat
    13201 tggttataat gaacgcttaa tgtcagttca ttataaccag taaggagaag gttataatga
    13261 accagaaaaa ccctaaagac acgcaaaatt ttattacttc taaaaagcat gtaaaagaaa
    13321 tattgaatca cacgaatatc agtaaacaag acaacgtaat agaaatcgga tcaggaaaag
    13381 gacattttac caaagagcta gtcaaaatga gtcgatcagt tactgctata gaaattgatg
    13441 gaggcttatg tcaagtgact aaagaagcgg taaacccctc tgagaatata aaagtgattc
    13501 aaacggatat tctaaaattt tccttcccaa aacatataaa ctataagata tatggtaata
    13561 ttccttataa catcagtacg gatattgtca aaagaattac ctttgaaagt caggctaaat
    13621 atagctatct tatcgttgag aagggatttg cgaaaagatt gcaaaatctg caacgagctt
    13681 tgggtttact attaatggtg gagatggata taaaaatgct caaaaaagta ccaccactat
    13741 attttcatcc taagccaagt gtagactctg tattgattgt tcttgaacga catcaaccat
    13801 tgatttcaaa gaaggactac aaaaagtatc gatcttttgt ttataagtgg gtaaaccgtg
    13861 aatatcgtgt tcttttcact aaaaaccaat tccgacaggc tttgaagcat gcaaatgtca
    13921 ctaatattaa taaactatcg aaggaacaat ttctttctat tttcaatagt tacaaattgt
    13981 ttcactaaat taaagtaata aagcgttctc taatttcaca agaggacgct ttattcttcc
    14041 caaaaattgt tcaatattta tcaataaatc agtagtttta aaagtaagca cctgttattg
    14101 caataaaatt agcctaattg agagaagttt ctatagaatt tttcatatac ttaacgagtg
    14161 ctttcacctt tgaatatagt ccttcccact tatcatcaca ctctccccga tagccttttc
    14221 tagctatatc cagtaaagtt acatgctctt taggtaaaag aggtatagcc cattctgcag
    14281 cgacatcttt cgaggtaatt tcaccagtag tcactgtttg ccacattcga gctagggtta
    14341 aaattacatt acgctcatca ccttttatcc cctcaattag ttctggcaaa gaatccttaa
    14401 ttgctcttcg aatatctgtc aaaggtacgg agacaagtat acttgaagaa tcaggaccaa
    14461 atagagaaat actattcttt cttgcttgtg ctaaaacaat agccaaatca ggatcatagc
    14521 ttggttcctg aatttgtcca ttctcaaatt cacccctgag ccactcaccg tatataaatt
    14581 ctctttttgg aggatattgc caagggacaa cttcactcct atttataacc gtaacttcaa
    14641 gtggtctaac agaatccgta tttccaatct ttcctgatat agtcattagt ctttctgtta
    14701 gtttttttcg agttaattga ggtaaactat gattcacgac gactagaaca tctacatcgc
    14761 tgttaatgcg taaaccacca tttactgctg aaccaaatag atatactcca actattgaac
    14821 ttccaaataa atcttttacg atttttaatg tttgaatcgc ttgatttggt atttttccgt
    14881 taatcaaatt gctcatgatt tcacctcgta actgattatg ttcatataaa gtttatattg
    14941 atactcaatt tacttaccct agattggaca tatacctaaa tgactgttca ataaagctga
    15001 ccgttagcgt ttaagtacat cctttcacaa tttgtctaca gattaataat tattctttat
    15061 tatacagatc tccatataat ttttgaattt ggttctgtaa ttttttattt tctttttcta
    15121 attccattac tcttcttttt aaggttttaa taaggatttc ctccgaacga gaacttttct
    15181 tgggttttga gactacattt gctgttattt gacgctcacg aagggattcg attctttgcc
    15241 taatatcgtg ttccttataa agccatgatt tagaaacatt agcttccttt gctattgaat
    15301 taaaattaat aactttacct tcaatcgaaa atttagaaat cgctttgtct actttttccc
    15361 ttgtcttttt tgatttctgc ttcgccaaac gtacaatttc tgttgtattt ctaacttgtt
    15421 tatccattga taattacccc gtcaaacttc caatgatttg ttctaaacgc tctttaacac
    15481 ggctattagt ctctacttgt ctttgccatt gtttatcctt agctatggct aataactctt
    15541 ctgtacgctc taactgttct tcgtgctgtg gtaagaattg cttactggta cagaagtgag
    15601 tgcaatctaa gcatgcattc gcatgtggac aaccacctgc tactactggc aatctacaat
    15661 aaccatttgg aagcacttgt gcatttatat ttttcttgaa ccattgaagc tctacatcat
    15721 cgacttcatt atcttcatct agatcaagca catctccatt attggtaacc agtttttcct
    15781 gaaatttagt aaattcattt tttagagttt catcaaagat atgagcgtat ctgcttgtca
    15841 tttctgggct ttcatgcccc aaaaatttct gcacaatatg ctggggcatc ccgttgttaa
    15901 tcattcttgt tcctactgta tggcgaaagg catgggcatg gaatctataa atctcacctg
    15961 atttatccac tatattttgc tcataagcta atttatttaa ctcacctcta aatgtttctt
    16021 gttttaatgg cgatccatct tttcttggaa agaggtattc actatctgga aattcctctg
    16081 aaactttatc ttcccgaact ttaataagta aagctacctc tttagatatt ggaactatat
    16141 gctccttttt cattttccat tgataatact ttaaaaagaa atctccatct ttgtcctcta
    16201 atagacagcc ttttttcaag gtgcacaatt cacttatcct cattccacat tcttgaacaa
    16261 tcatagtcat cgtagctata tattcgggta atttatcaag atgactgttc aattgctcta
    16321 ggacgaattc atctataaag cgtggttttg ctcttggtat tttcggatag tcctcagaat
    16381 aaattaatat tttggaagga acatcatccc attctagcct aagaagggta ctaaatagtc
    16441 cttccaatat agagatcctc ccagttattg tactaggttt tattcccatc atgtttagtt
    16501 cacttaaata tgcttcaatt tccactctcg ttaattggtg tactctctga acttgtttaa
    16561 atttcatgtc cagaaaatta aagaactctt taagtctttg ggcaatatca cttacatagg
    16621 aaaagctatc catgttcaat ctcaacttac aatatctttt tacaagttgt ttaaaatatg
    16681 tattccgaaa ccctttaaag ttaattgtat attcatattg tgttgggtta accttatcat
    16741 ctggcaaagg taagttacgt ctatcccaaa cgtctttatc ccactcctct ccatcaaaat
    16801 aaaagttctc ataaaactcc ataaattgtt ttagattagt aacatagtag gaattagctt
    16861 ttacaggtgt tttttcttga ttagcagtaa tcttataatt agtagtggta attctaacac
    16921 cccgttttgt caaataagtt ctatactccg tcattgcttt ttcaatagga acttcagtaa
    16981 ttgaagtaat gctaggatac tttaaatcta agaaatctaa cattttatta attactgttc
    17041 cttttctaat ccagacagtt tttgcattcc atattccatt gtttaaatgg taaaaataaa
    17101 aatatttcaa ttctgttctt aaccacagat ttttaacacg ttcaaaacga acccaacgat
    17161 tccttaaagc aggattctta cttaattcta tggcagaagg atgtggacat tttcttatat
    17221 cccaactatt attagcccaa aacccctgca tttcttcatt cattacagct atttttttgc
    17281 taatctcact ctgactaata attttccttt tactagaagc attcatttct tatgctcctt
    17341 tctctcgagg tatttattaa actcattttt catatcctga tctgaaagat gaacataggt
    17401 atttaacgtt gtctgaacat gtgcgtgacc taatctcttt tgaacgaacg caacatccca
    17461 tccttcccta attagctgcg ttgcgtgagt gtggcgaagc atatgtgatg taaattctat
    17521 tccagtcctt ttaactattc ttctaactag atcaagaaca ctttggtact ttagtggttt
    17581 cccaaaatag ccttctttta aggaaataaa aacataatca tgctccaatt cctcactata
    17641 ctcatatatc aagtaatctg tataaagtga cataagttct ttactcacat gtattgttct
    17701 ttccttcctt aatttaatat aagcttcatt aacattaaca tctctaggtg ttaaatggat
    17761 ttgattgtcc caagtgacaa tatcttcaag cctaagcgat aacacttcac cgattcttaa
    17821 accaccctca tacataagca ttaaaattaa tttatctctt ttcgtatgac aagcatcaat
    17881 aatttgctta acttcctctg atctcaatgt tcttatctgt ttctttttaa cccttaactt
    17941 taagacattc ttttggtatc tacccttatt aacatgatgt aaaaatcctt tgaaatttct
    18001 tccctttgct tgtttaaata catcaattga tttaaattct cctaatctac ttaaataatc
    18061 aagaaaactc ataactacat ttaaaattgt attcactgtc gtttcttctc ttatggcttt
    18121 ttttgactga agatcaatta catttgatgc tgaaggatat ctcaaccaac ctacgaagtc
    18181 tgctaacaac tcaaagttaa tatcattaag aataacacct ctctgttcca tgaactcgta
    18241 cagcaacttt aaatgatagc agtatgcttt aatggtatta ggagacttac cagtattatc
    18301 taagtattta ataaatttca ttactggttc tattagctgg tattctttat ctagtaataa
    18361 atacaatgga tacggcttat tctccacttc tatcctttga accttcacat gttcccacct
    18421 ctttaaatac tttaactact acaaattata aaccttgtta atttttattt aaagtaaaat
    18481 atccct
//
LOCUS       DAPCMT010000046        18189 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPCMT010000046 DAPCMT010000000
VERSION     DAPCMT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.046"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            163..2223
                     /locus_tag="RD743_001331"
     CDS             163..2223
                     /locus_tag="RD743_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDU0563154.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2306..2875
                     /locus_tag="RD743_001332"
     CDS             2306..2875
                     /locus_tag="RD743_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0563155.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3050..3856)
                     /locus_tag="RD743_001333"
     CDS             complement(3050..3856)
                     /locus_tag="RD743_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563156.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3994..5508
                     /locus_tag="RD743_001334"
     CDS             3994..5508
                     /locus_tag="RD743_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDU0563157.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5911..6894)
                     /locus_tag="RD743_001335"
     CDS             complement(5911..6894)
                     /locus_tag="RD743_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDU0563158.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6899..7546)
                     /locus_tag="RD743_001336"
     CDS             complement(6899..7546)
                     /locus_tag="RD743_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0563159.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7631..8263)
                     /locus_tag="RD743_001337"
     CDS             complement(7631..8263)
                     /locus_tag="RD743_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDU0563160.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8279..8830)
                     /gene="coaC"
                     /locus_tag="RD743_001338"
     CDS             complement(8279..8830)
                     /gene="coaC"
                     /locus_tag="RD743_001338"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDU0563161.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8827..9564)
                     /locus_tag="RD743_001339"
     CDS             complement(8827..9564)
                     /locus_tag="RD743_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDU0563162.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9561..10550)
                     /locus_tag="RD743_001340"
     CDS             complement(9561..10550)
                     /locus_tag="RD743_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDU0563163.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10808..11350
                     /locus_tag="RD743_001341"
     CDS             10808..11350
                     /locus_tag="RD743_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0563164.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11367..12452
                     /locus_tag="RD743_001342"
     CDS             11367..12452
                     /locus_tag="RD743_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0563165.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12464..13270
                     /locus_tag="RD743_001343"
     CDS             12464..13270
                     /locus_tag="RD743_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0563166.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13271..14098
                     /locus_tag="RD743_001344"
     CDS             13271..14098
                     /locus_tag="RD743_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0563167.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14095..15168
                     /locus_tag="RD743_001345"
     CDS             14095..15168
                     /locus_tag="RD743_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0563168.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15596..>18189
                     /locus_tag="RD743_001346"
     CDS             15596..>18189
                     /locus_tag="RD743_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDU0563169.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVS"
BASE COUNT         5873 a         3224 c         3715 g         5377 t
ORIGIN      
        1 aaaagtacat aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact
       61 ctcctaatag tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt
      121 actacttcat gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt
      181 tcaaaacaac taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa
      241 cactacgtta agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg
      301 catgtacccg ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag
      361 accactgatt cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta
      421 cttcctgcat tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg
      481 ttaattccta acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc
      541 ttttccgata tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg
      601 aatcatgctt tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa
      661 aaagattatt cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg
      721 atatctgatc tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa
      781 gtaatttcaa acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc
      841 ttgtcctatt ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt
      901 cgtgctgatc aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac
      961 gcccctaaag atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa
     1021 gaaatcaaaa tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta
     1081 acttatatag actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa
     1141 gcatctgctg cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa
     1201 cttcaagcag aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct
     1261 gaattttctg atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc
     1321 acttctattt ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag
     1381 atccttgaag tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt
     1441 acctttactt atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt
     1501 catccaaacg aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt
     1561 aaacccgtac cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt
     1621 tatttgacca aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca
     1681 ctccaagaac tccatgctca gtcactatta catgttttac cattaaacaa acagatagat
     1741 caaaaagaac aaaaaagacg agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat
     1801 gcagtttctc caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct
     1861 agaaaaatct cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt
     1921 tctggattgg aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt
     1981 gttgctaaac agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa
     2041 cgcttgtcaa ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt
     2101 ccgggaacac ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga
     2161 ggtatggcag gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc
     2221 tgatttatat ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat
     2281 aatggagacg aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg
     2341 gatgacaaga ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt
     2401 tcgattttat caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga
     2461 atttcaaacg acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga
     2521 agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg
     2581 taccgcttgg cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt
     2641 gaaaccagat taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga
     2701 atttcctacc ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca
     2761 ggcgattgct ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac
     2821 acctaatcca gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa
     2881 tggtgaaata agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac
     2941 ttttttgagt taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg
     3001 cagtgacaaa agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga
     3061 tttttttagg aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga
     3121 ggtaaccaaa tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag
     3181 tatccatgat tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat
     3241 ctgcattttg actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc
     3301 cgtctttact taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga
     3361 attgcagtaa tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg
     3421 aaaaacagaa ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt
     3481 tattgagcaa ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat
     3541 gtagaaaagt atcccacatt gaaaaatagc cataaaaatc acttaactct cctaaaactg
     3601 ttgaactaaa aataaaagat aacaaaatga tttctagcat atttggaatc tgtatcttta
     3661 atatcttttc cagaaaataa gggatttcaa ataaaattaa tgtcagtaat aaaagcattg
     3721 tatttcccca gtgttggttc cagacctgat ccactagcat aataacgacg atgatacgta
     3781 ataaaatata gattccgttt gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg
     3841 ttttcaaatg attcataaaa taactccttc atgtatatcc ttaattatac taagaagcgt
     3901 gtgtttacca aagctatatg acttaaagaa aaacagagtt gatattgtga aaaaaatcac
     3961 ttaatatagt ataaggagaa aagggggata caaatgaaaa aattagttgc tggtttatta
     4021 acagccgttg cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag
     4081 acaagttcta gtacgaagga atctgccgaa gtatcatcag gcgcttctga acaagtatat
     4141 acagatccaa aagaaatgaa agaatcttat gatgtcgtga ttattggagc tggtggtgca
     4201 gggatggcag cagctatcga agcaaaagat gctgggttga atccagtaat tttggaaaag
     4261 atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca
     4321 aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg ccttctatga agaaacgcta
     4381 aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat actttgtcga tcactctgct
     4441 gatgcggtcg attggctaga tcaaaacggt atcaaactgg acaacttaac gatcactgga
     4501 gggatgagtg aaaagaggac acatcgtccg tctgatggct cggctattgg tggatactta
     4561 gtggaggggt tattgaataa cgtccatgag cgggaaatcc ctatttttgt caacgcagat
     4621 gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca tgttcaagga
     4681 gaagaaccaa aagaggtcac cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc
     4741 agcaaagaat ttattgaaaa atatcgtccc gatttagctg attatattac gacaaatcaa
     4801 gaaggatcaa ctggagatgg tatcaccatg attgaaaaat taggtggaca aaccgttgat
     4861 atggataaaa tacaaattca tccaactgtg cagcaggatg agggggtctt gattggtgag
     4921 gttgttcgcg gagaaggagc aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa
     4981 atggatacaa gggataaagt atctgcagca attacagcat tgcctgaaaa atcagcttat
     5041 ttgatttttg accaaggcgt gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa
     5101 ggatatgtca caactggaaa gacaatggaa gaattagcag aaaaaatcga tttaccaaaa
     5161 gaaacactag caaaaacagt tgatggatgg aatacggatg ttgcggagaa aaaagatgct
     5221 caattcaacc gtgccacagc catggaacat gacttatcgt ctccgaatta ttatgcgatc
     5281 aaaatcgcac caggaatcca ttacacgatg ggcggtgtga aaatcaatac gaacactgaa
     5341 gtattagata aagaaaacca gccaattagg gggttatatg ctgctggtga agtcacaggt
     5401 ggcttacatg gaaacaaccg gatcggtgga aattcagttg cggagatcat tatttttggg
     5461 cgacaagcgg gcatacaagc agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa
     5521 ataaaaagag gttgtgaaat cagcgttttg agctaatacc gagggtcagc tgattgaaca
     5581 ctgtttagtg aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt
     5641 aaaaacagct tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg
     5701 aacaccgttt attcggaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc
     5761 tctaaacacg tataaacggc ggaagcagaa gaaattcctt cggaaataag ctgaaattca
     5821 caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct
     5881 gtcccagcct cttttttgat cagcatgatt ttattcagtt atcttcaaaa tgatttttcc
     5941 gatgtttttg tttgcttcca tatattcatg cgcttgtttg actgcgctga attggaatgt
     6001 acggtcaatg attggccgaa tcttgccatt ttctagaaaa ggcccagctt tttgcataaa
     6061 agtttgtgcg agctctgctt tatactgatc acttctaggt gtcaataatg taccaagcag
     6121 ttggacacgt ttcccaatca atacaccaag gtctatctca ggcacgatgg tcccacctag
     6181 cataccaatc aagacccaac gtccatcaac ggctatagaa gctaaattct tttcccagta
     6241 agaagcgccg atgaaatcca aaataacatc gactccccga ccttcagtga tttttcgtac
     6301 ctcttcttga aagtcttgtt ttttatagtt gatcaattca tctgcaccaa gttctttaca
     6361 aaaggcgagt ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct
     6421 agctaattgg atcgctgctg ttcccacacc acttgctcct gcatggatca agacagtttc
     6481 gccttcttgt aattttccaa gccagtaaag tgtttgataa gcagtcaaaa aaacttctga
     6541 gattccagca gcagaaacat agtctagcgt atcagggaga aggattgcac gatcggcagg
     6601 catcacagca tattcggcat aaccacctag attgactaat ccgtatactt ttgtcccagg
     6661 agtaaaagtg gagatatcac ttttattctc aactacctct ccagctactt cgactcccaa
     6721 aatagggtag ggaggtttca aagagactga ttctcttttc ataatatctg ttcgattgac
     6781 agctgctgta tggactttga tcagtaattc gccttgtttt ggtttaggaa caggcacttc
     6841 aatttgttct aatacatcta cagaaccagg atgcttgatt gaaatggcac gcatggatct
     6901 attcctcctt tgcccattct tggaatgctg tattcaactc tttgctacct tcagaaagta
     6961 ctagagtcgt acgagaaata tccgtttctt ccactagagc ggcaagtaca gcagcaactg
     7021 tagagcgagt gatcgttttt gatgccttct cattaggctt agttaattga atggaagtaa
     7081 cttcttcatc gttagtcaat gtgactggtt gaagaatgac aaaatctaag ttagttcgtt
     7141 tcagccattc atctgcataa tgtttagtaa tataataatc tgtcatggac tcaggccatt
     7201 ttgctggttc atctgcataa acagcactga ccatcagata gcgagagaca tgcgcatttt
     7261 cagctgcgat cactgttttg attgcgccat ccaagtctac ttgcagaaga ttctttcctt
     7321 gagagccggc agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc attttttcca
     7381 cggaccaagt taaatcaaaa gagacatacg aaacattctc tttttttact aatgtttggg
     7441 aagcaacatc tctcacccct gcaaaaattt catgggaact tggagctaaa tcttttatca
     7501 ggtgtcggcc aatttgtccg tttgcaccta caacaaaaat tttcatttgg atcatccttt
     7561 cgacgtcaaa tctttccatt caatagtaac accaaaagag aaaaaaatca gagatcggct
     7621 acgatgacta tcagttagta agagaagggt gcttgaagta tcttaacaag acattgcata
     7681 ctgctgcagt cgcaagacct gcgacgattc cttcaggtat accacttgta aaaataaccg
     7741 ctagaatagc tgaataaaca gcatttaaat ttgttccgat gactttggca taatcttgct
     7801 ggaataaaaa gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc
     7861 cagctaagaa taaagaaacg gaatctgctt tttgtttcat tagtttgcgc agacctttat
     7921 aaacgaaata aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt
     7981 tccagcttcc atgttctgtt ccgatgactg ggataaacgg agaaaagaca aaagacaaag
     8041 gtgtttggat aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag
     8101 cacctagtct tggtcctaaa acgatcgaag caatgattac aggaatatgc atggttgttg
     8161 cattgattgg accgataggg ataaaaccta aaggcgtaac agctaataaa atcaaaatag
     8221 ctaaaaacat cgcagttaat gtaaagtttt ttgtattttt cataagtact ccttttttct
     8281 aagcatttct ttcattaaga gtcagcatga cctgttcgat gatcgtctga atatcagcta
     8341 aggcaccttt tccaaaatct ccacaagcta aaagagattc tcttggttca atttcctgat
     8401 agccgacaga tctcaatgtt tccagattac gctgagtgat cgggttttga tacatattcg
     8461 tgttcatggc aggggcaatc aattttggtg ttcccactgg aagcgccatt gcgactgttg
     8521 aaagaagatc gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg
     8581 cgatgagaaa caagtcagct tgttttgcta gttcgatatg attgattacc tgcggatttg
     8641 attcttgcat gacgtctgta tggactgcgc gcttcgacag agattgcaag gttaatggcg
     8701 tgataaaatg tgtgctgctt ttagacataa tcacatctac ctgggcacct aatttgacga
     8761 attgacttgt aagatcagcg cttttgtaag cagaaatact tccagtaacg cctagtaaga
     8821 tctttttcat tttgattcct ccagtctaat tttctcagta atcaatgcag caatctcaga
     8881 tttactttga gcttcttcca ctgttccatc tttagataag agatagccgt ggtgtgtctc
     8941 gccatggatt tccgtcaaat catttgcgaa aataaaatca gttttgttat ttatcagact
     9001 attttttgcg actgccagaa gagattcctt tggtacatct actaatagtt tgaaaccaat
     9061 gatttttgct tttggctgcc attcacgtag ataagaaatg atttttggtg ttttcttcaa
     9121 aacgagcaca agatgctcgg tgttagaaga gatctttgaa acagtggtat ctgtctgttc
     9181 attttccgca aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc
     9241 cagctgttct tctgaaaaac taaaagcagg agtaaaatca ctgactgcca tactgtggat
     9301 aatcgcatcg tagtgttctt ttttcgttag ttcttctaat tgtaaaaata agtccagtgc
     9361 agattcgatc gtataaatcg ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt
     9421 agtcacgtaa tcgattgtgg tatccggatt ggcagctaaa taatcagcta atgcttgccc
     9481 taatcgtcct gtcgaatgat ttgtaatagc acgtacttga tcgatttttt cagtcgttcc
     9541 tccagctgtg attagaatct tcatttatgg tcacctacat ttactagtgt tcttccttgg
     9601 tgtgttccag caagtagttt agatgctgtt tcaggcaatt gatccagagt aatctcttgg
     9661 acgaccagtt catctgcaat ctgcaaatcc gccatctgtt gccataaaga aagacgttga
     9721 tctattggta cattgacaga atcaattcca atcaactgga tgtttcgcag aataaaaggc
     9781 aagacagtcg cgtttagttt gattccccca gcattgccac ataaagctac tgcaccgtta
     9841 taagagatca atggaagaag actgcttagc tgctcgccac ctactgtgtc gatgacatag
     9901 tcgatttgct gttttcctaa agcttttgtt gtctctggca gaaattcatc aggtcgtacg
     9961 acttgcaaag cacctagact ttttagccat gtgacagact cttcttttct ggaaaaggca
    10021 atgattgact ggaatcctag tttatgtaac agggctatcg ctgtactgcc tacaccgcca
    10081 gaagcaccag taacgacaac ttttttatcg gaggtcatac cttgacggat taaagcgttc
    10141 acacacaaca tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg cagcgtcata
    10201 ttttttggta gaggaactaa ccagtctcct ggtacttttt gatattggct ataaccgcct
    10261 ggatgggtga cgccaagccc aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt
    10321 ggattgttac tttggacgac agttccggat aaatcaatcc ctggagtcat cggatatgtt
    10381 cgtatcacct gaccagactc gctcattgcc aatgcatctt tataattgac atcagaatag
    10441 gcaattttta ccaagacttc attttcagcc aactcagtaa atggcagatc actgattgct
    10501 gtatggaaaa caggttcctt tattacctga aaagatcgaa aagcgttcat tcgtgagaca
    10561 ctccttttaa aattttcttt tatcatacca taaagcctac tcttttattc ttgttcttcc
    10621 gtaaatttag acaaaaatta ggtgaaaaat aaagaatcaa acaactagaa tttattccaa
    10681 aagataaaga aatattcagc aaaaaaaatt ttacaaaggg ttgccttttt caataaattt
    10741 cgtatactaa tacgagtggt agtaaacagg aagtttacta ttagaaaact tcgattcgga
    10801 ggaaattatg gaaattggtg agaagttaag aaatttgcgc atccaaaaga atttgacaca
    10861 agaagaacta ggcgaacgca cagatttgtc aaaagggtat atttcccagt tggaacgaga
    10921 tttaagttca ccatcaatgg aaacattctt ttctattctt gaagtcttag gtgtgactcc
    10981 agaagaattt tttcatcaag aagcagcgaa tctgcaagtg gtttattcaa aagaagatca
    11041 tacggtttat ttcgatgaag aaaacgggta tgaattggaa tggctcgtgg ctgattcgaa
    11101 tgaaaaagaa atggagcctg tcttgctgac ttttgaaggt gcaggagaat ataagacgtt
    11161 tgaaccttca ttatctgaaa cgtttatttt tgtattagaa ggggaattag agttaagctt
    11221 aggagagacg gtctatgtgg caaaagcagg acagtcgatc tattatcaag cgacaaaaca
    11281 acatcaatta aggaatcatc tcaaaaaacg aacaaaagta ttgattgttg caacgcaatc
    11341 atatttgtaa tatagggggt aaaaacgtgg ggaaaccaat catttcattt gatcaagtag
    11401 tgaaacgcta tgatgatgaa acagtattaa aaaaagtaag ttttgagatc gaacaaggaa
    11461 aattttatac actgcttggt ccttccggct gtggtaaaac aacgatccta cgtattatag
    11521 ctggattcac cgaagctaca gaaggggaca tctattttga agggaaacgg ttgaatgatt
    11581 tgccagctaa taagcgacaa gtaaatacag tcttccaaga ttatgcactt tttcctcata
    11641 tgaatgtttt tgataacgtt gcttttggat tgaagatcaa gaaaatgtcc aaatcagata
    11701 tcgaaaagaa ggtaaaagat gctttgcgta tggtccagct tcctggatac gaaaaacggg
    11761 agattagtga aatgtctggt ggtcaaagac agcgtgtagc gattgcacga gcaattgtaa
    11821 atgaaccgaa agtattgcta ttagatgaac cgttatcggc gcttgatttg aaactacgta
    11881 cagatatgca gtatgaactg cgggatctgc aacaacgatt aggaatcacg tttatttttg
    11941 ttactcatga ccaagaagaa gcgttggcaa tgagtgatga gatttttgtc atgaataaag
    12001 ggaaaatcgt ccaaagtggt acacccgtag atatttatga tgagccaatc aatcatttcg
    12061 ttgctgattt tgttggggag agcaatatcg tggacggtgt aatgatcgaa gataatctag
    12121 ttgagtttgt tggtaagcaa tttgaatgtg tcgatggcgg gatgcgtcta aatgaaccag
    12181 ttgaagttgt tttacgccca gaggatttga cgatcacgac accagataaa ggaaaactag
    12241 tcgtaacggt cgatactcag ctgtttcgtg gtgtccatta tgaaatcatc tgttatgatg
    12301 aacagcaaaa tgaatggatg gtccattcaa cgaaaaaagc taaagaagga agcaaagtag
    12361 gattggcctt tgagccagaa gatatccatg tcatgcgttt caacgagtct gaagaagaat
    12421 tcgatgcacg tcttgacagc tacgaagaat aggggggaca agaatgaaaa caatgcgcag
    12481 gatctattcc gtcccttata ttttgtggct cggtttattc gtgatcgcgc ctgtattaat
    12541 gatcatttat caatcttttt ttgatatgaa tgggcaattc acgctgaata attatctatc
    12601 ttattttacg tcggggacat cgctttctat gacgatcaat tctgtatggt atgcattttt
    12661 gatcacgttt ttcacgctga tcatcagtta cccgactgct tattttttaa caaaactgaa
    12721 gcacaagcaa ttatggctga tgctgattat tttgcctaca tgggtgaatc tgctgcttaa
    12781 agcttacgca tttattggga ttttcagtgt gcacggcagc gtgaatcatt tctttgattt
    12841 tatcgggatc gggccgaaac agatattatt cacagacttc agttttctgt tcgtggcaac
    12901 gtatatcgag attccattta tgattatgcc aattttcaat gcattggaag agatgaatcc
    12961 ttctttgatc agtgctagta gagatttagg cgcgaatagc tttgagacct tccgacgagt
    13021 gatttttccg ttatcattaa atggtgtgaa aagtggtgtc caagcagtat ttattccttc
    13081 tctttcttta ttcatgctga ctcgtctgat tggcggaaat cgggtgatca ctttaggaac
    13141 ggctatcgaa cagcatttct tggttacaca aaactggggg atgggttcta caatcggtgt
    13201 gattttaatc attgctatgt tcatcgtgat gctcctgaca ggcgagaaga aaaaaggagg
    13261 tcgctcataa atgaaaaaat ttaaatggtc gtatctttat ttagggatcg tctttttatt
    13321 cttatatgca ccgatcttct atctgatttt ttattccttc aatgaaggag gaacgatgaa
    13381 cagctttaca ggatttactt gggaaaatta tgaagctgtt tttgaagata cacgtttgat
    13441 cattatcgta ttgaatacgt tcatgctggc tttcttatct gcattgatcg ccacgatcat
    13501 cgggacattt ggggctatgg gaatctatta cacgaaaaaa cgacgtgcac ggacagcgtt
    13561 gttgagcatg aataacatct tattagtctc tcctgatgtt attatcggtg ctagtttctt
    13621 gattttcttt acactactag gattcggatt aggttttcag agtgtattgc tttcccatat
    13681 tgctttcagc attccaatcg tcgtcttgat ggtcttgcca aaattgcagg aaatgaacga
    13741 ttcaatggtc gatgcggcta gagaccttgg tgctaataat ttccaagtag tcaaaaatat
    13801 cattttgcct tatctttctc caggaatcat agctggatat tttatggcat ttacctattc
    13861 tcttgatgat tttgcggtta catttttcgt cacaggaaat ggctttacga cgctctcagt
    13921 agagatctat tctcgtgcaa gacaaggaat cagtttagaa atcaatgcgc taagtgcgtt
    13981 agttttcttg ttctccatga tattggtcat tggatactac tttattagta aagaaaaccg
    14041 tccaaaacgg ctgaagaaaa acagaagagt acgcgagggg gtggcacggc tgcgatgaaa
    14101 aaattacaat ctctgttttt aggtattctc atcattattg cggtgctgct gcttggtgtt
    14161 cgtcaacttg aaaaaacaag tggcatgagt ggagcaaagg tacttaccat ctataactgg
    14221 ggagattata tcgatcctag tttgatcacg aaatttgaaa aagaatatgg atacaaagtg
    14281 aattatgaaa catttgattc taatgaagcg atgttcacaa aaatccagca aggtgggaca
    14341 aactacgaca tcgctatacc aagtgaatat atgatccaaa aaatgatcaa agaaaaacta
    14401 gtacttccat tagatcattc gaagatcaaa ggattaaata atattgatcc acgattttta
    14461 gacttggatt ttgatccaga taatcagtat tcgattccat atttttgggg aactttaggg
    14521 atcgtatata acgataaatt ctttgatgca aatcaaatta agcattggga tgatttgtgg
    14581 aagccggaat taaaagacag tttgatgctg attgacggtg cacgagaagt tatggggctt
    14641 tctttgaata gtttaggcta ttctttgaat agtaagaata tggcacagtt gacagaagca
    14701 gcaaataaat tgaatcattt aacaccaaat gtcaaagcaa tcgtggcaga tgagatcaaa
    14761 atgtatatga tcaatgaaga agcctccgtg gcagtgacat tttctggcga agctgccgat
    14821 atgatgtggg aaaatgaaca tttacattat gtgatcccac cagaaggatc caatctttgg
    14881 tttgataata tcgttatgcc aaaaaccgtg aaaaataaag aaggtgccta tgatttcatc
    14941 aactttatgc tagaaccgga aaatgcggca caaaatgcag agtatattgg ttattctaca
    15001 ccaaatgaag aagcaaaagc gttattgcca aaagacattt cgtcggacga acaattctat
    15061 ccatctgatg acacgatctc ccatcttgaa gtctacaaag atctaggatc gaaataccta
    15121 ggcatctata atgacctatt cttagaattc aagatgtata ggagataatg gcaaaactca
    15181 gtaagaatgg ggacttatca acgttctaaa gagctaaaaa tcgtcctttt tctatctgag
    15241 ttttccaatc ttctaccaaa agcggatgaa acgccgcttt ttttgtgtta ttttattttt
    15301 tctaccacgt ttttctacca gagaaaagtg gacaaaacca ttgtaaaata gggcccgaga
    15361 cgggctagta aatctaagat tgaaaaagtt acagaaggat aaagtaattg gaaactatgc
    15421 aaatatactt aacccttctg taatctatta ataaaactaa tatatgaatc cagttcaata
    15481 tttgtagcta tgaaaaaaca taaaaatcat attagataaa aactaaattg gtagataaat
    15541 tattttgtca gatatctgag gcaaatattc gactatttgt gtttcaaagt aaaaattgga
    15601 atacaaatat agaatagaca tattgcagcc gatagtttat tatgctttta gacacattaa
    15661 actggaggaa aaaatgaaaa agagcgctaa agatcgagtt tttgatgagc tgatacgact
    15721 aacagggaat atgtcttctg tcactgccga gaaaatttcg aagaatctag gtatctctcg
    15781 tcagaatgcc agccattatt tgacgagatt agtggaagat aaaaaggtcg agaaattacc
    15841 gggaaagcct gttttgtgga agcctttaga tgaatatgcg gttgtagata atacggagca
    15901 aataaacgaa gcctttcatt ctgtagttgg tcatgacgga agtctgagag aggtcattca
    15961 aaaatgtata gccgctgtca aatatccgcc taacggactc agtgtattaa taaatggagc
    16021 cacgggtgtt ggaaaaagtt ttttagccac aaaaattttt gaatacgcta tccatgagca
    16081 aattattgaa aaagatgcac catttgctat cttaaactgt gccgattatg cggacaatcc
    16141 cgaactgctt tcggcaacac tgtttggata caagaagggg gcctttacgg gggctgaaaa
    16201 ggataccgat ggtttattgg caacggccaa caacggctat ctttttctcg atgaggtcca
    16261 tcgcctatcg aaagaaaatc aagaaaagct ctttctattt attgatactg gaaactatcg
    16321 tccagtcggt gaaaatgtaa attggcattc tgctaaggtt cgttttattt tcgccacgac
    16381 ggaaaaaggt gaaaattatt tattagacac ctttgatcgg cgtattcaaa ttagtgttat
    16441 gttgccaact tttgaagatc gccccattcg agaacgatta gagctgattc aattattttt
    16501 tcaaaatgaa gcggatgttt tacaaaaaga tattattgtt tcaagagaag ccttgtcttt
    16561 aatgttatct catcccttta gtgggaatat tggtaagctg aagaatatca ttaaaatcag
    16621 ttgtgcggat gtgtatagtc gaacacaaga agaaccatta acgattggga aaaatgagat
    16681 cttgattcag ttaaatatgc tggaatccga agtagaaagc ttgccgattg ctagtttatt
    16741 gataaagaaa aatcaaaaga taaaaacgga tcgaatcaaa ccgtttgatt atgattcgat
    16801 tttggaaaaa attactcagc tgataaatga aggtaatttc aatgaaaatt tttcttcgat
    16861 aaagaatgaa gtacagcatt tggtgcatca cattgataag gattcttttt tatcaatgag
    16921 tagaaaattg atgattcaga tgtttagaaa ggtctgggta tccgttttag gaaaaacgta
    16981 cggattgacg acgagtggac ctgttgcaga aattattagc agatcctatg tcatttcccc
    17041 aagctataag acagacttaa ccgggataaa tcgtacatta gctgcaaatc tacctcggac
    17101 gacttactta tgtaatcagt tcttggagca tttaccaccg ctctcagaaa aggacgagaa
    17161 ctttctaaga ctgcttattg tcgtgacttt atctgattat gtagatgaat ccatcgagct
    17221 gaagggcctg ttgcttgctc acggagatag tacggctagc agcattcaag cagtagtgaa
    17281 tcaattatgc ggcaattatg tttttgaagc gttggatatg ccagtagaga gtaatgtgtc
    17341 agagattgtc gaatcgacaa ggaatttcgt gaaacggcaa gtgaatacag atagtctagt
    17401 gctgatcgta gatatgggct ctttaaatca aatttacaca cagatcaaga acgatttaaa
    17461 aggggagtta cttcttctga ataatttgac gacttccatt gcacttgata ttggcttgaa
    17521 gatgacgatg aatgcgccat ttaaacagat tgcggaggat gcagcagaca agtattcaat
    17581 caatgttcaa tactttgaag gcttttctca aagtagcaac attatcattt cctgtatgtc
    17641 gggtttgggg atttcggaca aattgaaaga tgtttttcgt catcatttat cggatgaatc
    17701 catcgaagtt ttcacgaagg actacagaga actacgtgaa ttaatcgacc aaaatgatga
    17761 aacttatttt gacaagacaa aattggtcat cacgacttcg gatcttccac gttccttttc
    17821 aattcctaat attaatgttt atgacatctt agacggtgat ggagcagcca atttggagca
    17881 tattttgagt cctgaaatga agccggcaga atttaaccgc ttgattcagg agctagtaaa
    17941 attcttttcg ttggaaggaa ttgcggaccg tttgaacttt ctgaacccaa cgatcgtgat
    18001 gagtgaagtg gaaacagtca tttctaaata tgaaaattac tatcatttca ctttaaatgg
    18061 ccgtgtaaaa ctaaatctct atatgcatac ggcattgatg atggaacgtt tattcttggc
    18121 tcggacagaa aaatctgaag aaccgaatta tgatttgaaa ccagatgaag atgaatttta
    18181 ttatgtttc
//
LOCUS       DAPCMT010000047        18186 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPCMT010000047 DAPCMT010000000
VERSION     DAPCMT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.047"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..152
                     /locus_tag="RD743_001347"
     CDS             <1..152
                     /locus_tag="RD743_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0563170.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="RD743_001348"
     CDS             complement(230..763)
                     /locus_tag="RD743_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDU0563171.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="RD743_001349"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="RD743_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDU0563172.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="RD743_001350"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="RD743_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDU0563173.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="RD743_001351"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="RD743_001351"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDU0563174.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="RD743_001352"
     CDS             complement(4488..5066)
                     /locus_tag="RD743_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDU0563175.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="RD743_001353"
     CDS             5388..5858
                     /locus_tag="RD743_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDU0563176.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="RD743_001354"
     CDS             complement(5966..7192)
                     /locus_tag="RD743_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDU0563177.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="RD743_001355"
     CDS             complement(7946..8146)
                     /locus_tag="RD743_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0563178.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="RD743_001356"
     CDS             complement(8280..8888)
                     /locus_tag="RD743_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDU0563179.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="RD743_001357"
     CDS             complement(8973..9443)
                     /locus_tag="RD743_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDU0563180.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="RD743_001358"
     CDS             complement(9491..10405)
                     /locus_tag="RD743_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDU0563181.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="RD743_001359"
     CDS             10551..11354
                     /locus_tag="RD743_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDU0563182.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="RD743_001360"
     CDS             11532..12479
                     /locus_tag="RD743_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDU0563183.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="RD743_001361"
     CDS             complement(12547..12840)
                     /locus_tag="RD743_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDU0563184.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="RD743_001362"
     CDS             complement(12868..13533)
                     /locus_tag="RD743_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDU0563185.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="RD743_001363"
     CDS             complement(13673..14305)
                     /locus_tag="RD743_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0563186.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="RD743_001364"
     CDS             complement(14312..15379)
                     /locus_tag="RD743_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDU0563187.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="RD743_001365"
     CDS             complement(15376..16107)
                     /locus_tag="RD743_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDU0563188.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="RD743_001366"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="RD743_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDU0563189.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="RD743_001367"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="RD743_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDU0563190.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5399 a         3687 c         2906 g         6194 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg ttgatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actata
//
LOCUS       DAPCMT010000048        17224 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPCMT010000048 DAPCMT010000000
VERSION     DAPCMT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.048"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            1..369
                     /locus_tag="RD743_001368"
     CDS             1..369
                     /locus_tag="RD743_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDU0563191.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="RD743_001369"
     CDS             549..1490
                     /locus_tag="RD743_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDU0563192.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="RD743_001370"
     CDS             1559..2290
                     /locus_tag="RD743_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDU0563193.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="RD743_001371"
     CDS             2434..4614
                     /locus_tag="RD743_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDU0563194.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="RD743_001372"
     CDS             4634..5116
                     /locus_tag="RD743_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDU0563195.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="RD743_001373"
     CDS             5132..5893
                     /locus_tag="RD743_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDU0563196.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="RD743_001374"
     CDS             6024..7358
                     /locus_tag="RD743_001374"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDU0563197.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="RD743_001375"
     CDS             7665..8150
                     /locus_tag="RD743_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDU0563198.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="RD743_001376"
     CDS             8143..8826
                     /locus_tag="RD743_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDU0563199.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="RD743_001377"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="RD743_001377"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDU0563200.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="RD743_001378"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="RD743_001378"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDU0563201.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="RD743_001379"
     CDS             12442..13071
                     /locus_tag="RD743_001379"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDU0563202.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="RD743_001380"
     CDS             13123..13455
                     /locus_tag="RD743_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDU0563203.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="RD743_001381"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="RD743_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDU0563204.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="RD743_001382"
     CDS             14281..15654
                     /locus_tag="RD743_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDU0563205.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="RD743_001383"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="RD743_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDU0563206.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="RD743_001384"
     CDS             16709..17149
                     /locus_tag="RD743_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDU0563207.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5875 a         3008 c         3795 g         4546 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggtagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tggg
//
LOCUS       DAPCMT010000049        16853 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPCMT010000049 DAPCMT010000000
VERSION     DAPCMT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.049"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            303..1412
                     /locus_tag="RD743_001385"
     CDS             303..1412
                     /locus_tag="RD743_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDU0563208.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1495..2391
                     /locus_tag="RD743_001386"
                     /pseudo
     CDS             1495..2391
                     /locus_tag="RD743_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2407..3231
                     /locus_tag="RD743_001387"
     CDS             2407..3231
                     /locus_tag="RD743_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0563209.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3263..4573
                     /locus_tag="RD743_001388"
     CDS             3263..4573
                     /locus_tag="RD743_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDU0563210.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4721..5980
                     /locus_tag="RD743_001389"
     CDS             4721..5980
                     /locus_tag="RD743_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDU0563211.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5990..6862
                     /locus_tag="RD743_001390"
     CDS             5990..6862
                     /locus_tag="RD743_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0563212.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            6874..7704
                     /locus_tag="RD743_001391"
     CDS             6874..7704
                     /locus_tag="RD743_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0563213.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            7744..9927
                     /locus_tag="RD743_001392"
     CDS             7744..9927
                     /locus_tag="RD743_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDU0563214.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            9988..11208
                     /locus_tag="RD743_001393"
     CDS             9988..11208
                     /locus_tag="RD743_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDU0563215.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            11205..12665
                     /gene="gtfA"
                     /locus_tag="RD743_001394"
     CDS             11205..12665
                     /gene="gtfA"
                     /locus_tag="RD743_001394"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDU0563216.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            12742..13848
                     /locus_tag="RD743_001395"
                     /pseudo
     CDS             12742..13848
                     /locus_tag="RD743_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            14170..14379
                     /locus_tag="RD743_001396"
     CDS             14170..14379
                     /locus_tag="RD743_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDU0563217.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            14372..14521
                     /locus_tag="RD743_001397"
     CDS             14372..14521
                     /locus_tag="RD743_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDU0563218.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            14675..15340
                     /locus_tag="RD743_001398"
     CDS             14675..15340
                     /locus_tag="RD743_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0563219.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(15624..16709)
                     /gene="xerS"
                     /locus_tag="RD743_001399"
     CDS             complement(15624..16709)
                     /gene="xerS"
                     /locus_tag="RD743_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDU0563220.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         5702 a         2551 c         3256 g         5344 t
ORIGIN      
        1 atcgattaaa aaagtaaaca tccaaaaaag ggtgtttact tttttgttta ctaatatttt
       61 tagtaaaaaa tctcgacaaa acattaagta aacagttatg gtgttgttaa caaaaagaaa
      121 acgcattcaa aacaggagga attaagatga aacatttcaa aaaaatagct ttaacaggga
      181 tagctgtttt aactgggaca ttactcgcag catgtggtgg gggagatagt aattctaaag
      241 acgggcaagt aactttaagc tatgcgagtt gagatactga ccaagctcca ggactaagaa
      301 aaatgttaga tgaatttgaa aaacaaaatc ctgatatcaa agtagaaatg gagacaacgc
      361 cttgggatca atattgggtt aaattagaag ctgcaacaac gggaggtaat atgccagatg
      421 tagtaaccat gcactctagc gaatcctata aatatatgtc acagaatgct ttgatgggac
      481 ttgatgatgt tatcaaagaa aatgatattg acatgggaaa ctttactgaa ggaattgctg
      541 atttctatac gcttgaagac gaactatacg caatccctaa agatgcttct gtagtggggc
      601 tatggtacaa caaagaatta tttgataatg caggtgtcga ttatccagat gaaacatgga
      661 catgggaaac actcagacaa gctgcaattg atttaacaga tgaatcaaaa ggcatctatg
      721 gttttgcatc acatattgga acagagactg gctactggcc atttatttat caaaatgggg
      781 gagaagtatt tgccgatgac aatacaaaat caggtttgaa taccccagaa gcacaagaag
      841 ctttagcttt ttatcgagat attgcacttg ttgatcatgc ttcaccaaca attacagaat
      901 tacaagatcc tgataagtta agtcgttttc aatcaggtag attagcaatg atgattgaag
      961 gaaactggtt tgcaccggga tttttgaaaa atgactatct agttgaaaat gttgcttctg
     1021 ccgtgttacc taagggaaaa gttaatgcaa caattacaaa tggacttggt tgggctgcca
     1081 gtgcgagtac aaaataccca gaagaagtga aaaaactaat ggcattttta gctagtgagg
     1141 acgccaataa aatccaagcg gaaacggggg catcaatccc agcactaaaa ggctttgaag
     1201 acgagtgggc tgcttcttca gatacaattg atttgagtgc ttttggaaaa atgttggagt
     1261 atggagtccc tcgtccatac aataaagatg gattacgagc tgaacaagca gaagttcaaa
     1321 ttattaaccg tattctgtct ggtgaagtaa ctgttgaaga tggaaccgct caaattgaaa
     1381 aagaagtcaa tgcgatattg agcggaaatt aaattagtta attatacaat atacaggcga
     1441 aaggattcta tttcgtctgt atattaatgg atgaatacca aaaaggggga tattatgaaa
     1501 aatcgaattt ttggaaaata tcgtaaagat caagtgatat ggggctatat ttttattttt
     1561 ccaacggcat taggattaat tttattaaat atatggccag caattcaaac agcaatttta
     1621 agttttcaaa aaacggtagg gttcggtgat actgaatggg tcggattaag aaattaccaa
     1681 aaaatgtttg cagacggtga agtttttcag tctttattaa acacattaat ttatgcagtg
     1741 gtttcgatac ctagtattgt tgtattatcc ttgttggtgg cagttttaat gaatagaaaa
     1801 gttaaaggat tgtcgattta tcggacaatt tattttctac cagtcgttgc tgcaccatcc
     1861 gcagtcgcaa tgatttggcg ctggatgttc aataatgatt atggactgat taataacatg
     1921 ttggctaaaa taggtctcga tggaccagca tggttaacgg atccatcgat tgcgatttat
     1981 tctattatca ttgtaggagt ttggagtgct attggttata acatggtgtt attactagcc
     2041 ggcttataag aaattcctaa agattattat gaagcagcgg aaattgatgg tgctagtcca
     2101 atccagcagt ttttcaacat tacgttacca ttagttacac caacattatt ttttgtagtt
     2161 gtaacaacag ttatcaatgc tttccaagta tttgatgtta tttttatgat gattactcct
     2221 aatagtaccg ccatgtttaa atcacaatca ttagcctatt tattctataa acattcattt
     2281 attttaaatg acaagggata tggttcggca atcgttatgt tccttttagt gattatatta
     2341 attattactg caattcaaat gaagttgcag aaaaagtggg taaactatta aggaggctat
     2401 caaattatga aaaataaatt tttttcaaat gcgccaatcc atatcatgct tattttaggt
     2461 ggtattgcaa tggcattacc ttttatttgg atgattttaa cttcagggaa aacgttggtt
     2521 gaatctaccc aaattccacc tacaattttc cctgagaaat ttcagctaag taactatcaa
     2581 caagtacttg atttattacc attcatggct ttttatatca atacattttt aatggttgtt
     2641 ggacgagtga ttggctcggt tttattttca gcaatggctg catatgcttg tgcgcgtcta
     2701 agttttccag ggaaaaatat ccttttcagc ttagtactta ttcaaatgat gattcccggt
     2761 tccattttta ttactccaca gtatttatta gcacaaaaat tgggtatttt aaatacaatt
     2821 ccagcattaa tattaccagg ggtagttagt gcgtttggta cgtttttatt aagacaattt
     2881 ttcttaggtt tacctgatga attggaggaa gcggcctatt tagaaggtgc caatatttgg
     2941 acgatctttt tcaaaattat gttgccgctt gctaaatcag gattggttgc acttggaata
     3001 ttcacagcgt tgtttgcatt taaagattta atgtggccat tgattgtcaa tacttcaatt
     3061 gataagatgc ctttagctcc gggattagca aacttacaag gacaatatgc cacgaacttc
     3121 ccacaattaa tggcaggttc gttattagca atttggccaa tagttgtgat attcatcgtt
     3181 ttccaaagaa aattcattga aggaattgct acaacaggta gtaaattata aaactattaa
     3241 ttagtgattg gagagtaagt acatgacaaa aattacattt attggtgcag gaagtactat
     3301 ttttgcaaaa aacgtattag gcgatgcgat gttaactcca agtctacaag atgcacatat
     3361 tgcattgtac gatattgatg aaaaacgact aaaagaatca aatttaatgc ttaaaaatat
     3421 taataaaaat tcgaatgaaa atcgtgctga tattgtttct tattatgatc gcaaagaagc
     3481 gttaagagag gctgattttg ttatcaatgc tattcaagtg ggtggttata aaccttcaac
     3541 agtgattgat tttgatattc ctaaaaaata tgggttgcga caaactattg gtgacaccat
     3601 ggggattggt gggattttta gaaccctacg gacactacca gtaatgttag actttgctaa
     3661 agaaatggaa gaagtttgtc caaatgcttg gttattgaac tatacaaatc cgatggcgat
     3721 gttaaccatg ggtgttttaa aagcgacgag tattaagact gttggtttat gtcatagcgt
     3781 tcaagtatgc gtaccagaat tgtttgagca tttaggaata gccgatcaat atgacttaaa
     3841 tgactttcaa tggaaaattg caggcattaa tcatatggca ttcttgttgg aaattacaaa
     3901 aaatggggaa gatttctatc caacaattcg tcagttagct gcagaaaaac caaatccaca
     3961 tccagattct gtacgttttg aattgttgaa acgttttggt tactatatta cagaatcaag
     4021 tgaacacaat gcagaatacc atccatactt tattaaatca aaatacccag aattgattga
     4081 cgaattaggg attccgttgg atgaatactt acgtcgttgt gaacaacaaa ttaatgattg
     4141 ggaaagcatg cgagaggaca ttgtaaataa tggggagtta acacatacga gaagccgtga
     4201 atatgcttca tatatcatgg attctattac gacaggaact ccgtcagtga ttgcaggaaa
     4261 tgtcttaaat aaaggcttaa tcacaaactt accgacagat gtttgtgtgg aagtaccttg
     4321 tttagtcgat aaaaatggcg tgcaaccaac ctatgtcggt gaattgccaa cacaattagc
     4381 tgctttaaat cggacgaata tcaatgttca agaactgaca gtagaagccg ctttaacact
     4441 agataaatca aaaatttatc aagcagcgta tttagatcca catctatcat ctgaattgtc
     4501 tttaacagat atcaaaaatt tagtggatga tttaatcgaa gcacatgggg actatttacc
     4561 tgaatataat taaaaagaag ctgaaagagt aaggaatttt caattcctta ctcttttctt
     4621 agttaaattt agtaaaaata ttgacaaaac acttagtaaa cagttacatt agtgttaaca
     4681 aaaagaaaac gcattcaaat tgtataagga ggagcataaa atgaagtggt ctaaaaaaat
     4741 tattttagct tgtatagcac taggagcaac gatctctttg actgcttgtg gaaaaaatca
     4801 ggaagatgca agcggtaagg tagaaataga gttttttaat caaaaaaaag aaatgactca
     4861 aacgattcag gagattgcta aagattttga agcaaaaaac cctgatattc atgtaaaagt
     4921 tgtggatgta ccgaatgctg gtgaagtaat taaaacacgt atgcttgcag gcgatgtacc
     4981 agatgtcatt aatctttatc ctcaaagtat agaattgaaa gaatgggcga aagctggtta
     5041 tttagaggac ttaaccgaag aaccgtattt agagaatatc aaaaatggat atgcacaacg
     5101 ttttgctatt gaggatcgtg tatatagcat accattaacc gctaatgtct atggttttta
     5161 ctataacaaa actgcttttg aggaaatggg gattcaagca ccagaaactt gggcagaatt
     5221 tgaaaaaata gtagctgaga ttaaagatca aaacaaagta ccgtttgcga ttgcaggagc
     5281 agaaggctgg actttaaatg gttatcacca actagctata gcgactgctg ctggtggtga
     5341 agaggaagcg aacaatgttt ggcgtttctc agaagttaat ggaattaatg ctgaaagcaa
     5401 agaaatgcaa aaagatttta atcgattaga tttgttaaga gaaccaaaag ctttgcaaaa
     5461 caactggcaa ggtgccggtt ataacgatac agtagttact tttacaaaag gtgatgcgtt
     5521 gattatgccg aatggttcat gggcaatgcc gatgattcat tcacaaaatc cagattttga
     5581 agtaggaact tttccttttc cagcagatga agcaggtcaa agtctaacga ttggcgcagg
     5641 agacctagcc ttatcagttt ctgcaacttc tcaacataaa gaagaagcca aacgttttgt
     5701 agaatatatg acaacacctg aagcgatgca gaaatattat gatgtagatg gtgcaccgtg
     5761 tgctgttgag ggcgttatcg agaatactca ggattcacca ttaagtggtt taactgaact
     5821 tgcttttact gatcgtcatt tagtttggtt agccaaagat tggaatagtg aaaatgattt
     5881 ttatacctta acaacaaatt atttacacaa tggcaatcaa aaagccatga ttaacgcatt
     5941 aaatgctttc tttaatccaa tgaaagctga cggaaggtag gaaaataaaa tgaaaataaa
     6001 agaaatttta aatcgttact gggcaatcat ttttgtagca attccaatta ttttgcagat
     6061 gatttttttc tactttccaa tggttcaagg tgctttttac agtttaacta attggactgg
     6121 tctgacttat aattttgatt ttgtgggttt gaacaattat aagattcttt tttctgatcc
     6181 gaagtttatc aaatcgattg gcttcacttt aattgtgacg ctttgcatga ttgctggaca
     6241 aattggtttt ggtattttga ttgcgcgggc gttgaacgca aatattaaag ggaaaacatt
     6301 ctttagagca tggttctttt ttccagcggt cttatcgggc gtggttgttt ctttagtatt
     6361 taaacaaatt tttaactatg gtttaccaaa aattggtagt atgttaggca ttgaattttt
     6421 acaaaccagt cttttaggaa caacatgggg agcgattttt gcagtaattt ttgttttact
     6481 ttggcaagga attgccatgc cagtcatcat tttcttagcg ggttttcaaa gtattccaca
     6541 agaaattatt gaagctgcta gaatggatgg ggcgaatgat aagcaaattt tttggaatat
     6601 tgaaatcacc tacttattac caagtatttc aatggtattt attatggcat taaaaggtgg
     6661 cttaacagca tttgatcaaa tttttgcttt aactggtggt ggaccaagcg atgccacaac
     6721 atctttgggg ttacttgttt ataattatgc ttttaaaaac aatcagtttg gttatgcgaa
     6781 tgctattgct ttagtcttat ttatcttaat tgggattgtt tcactggcac aattaaaatt
     6841 atcgaaaaaa tttgaaatct aggaggtttc ccaatgaaaa catcaaaaga aaaaaatatt
     6901 tctaaatact tgcttttatt attagggggc ttcatgattt tagttccctt agtttcaacg
     6961 ctattcggtt catttaaaac aacacccgat attatgaata atttctttcg ttttcctaat
     7021 ccagcgactt tggaaaattt ccgaagattg ttagcggatg gaattggtca ttacttttgg
     7081 aattcggcaa tcatcacaat cattgcagta gttgctgtga cagcaattat tcctatggct
     7141 gcttattcac tagcaagaaa aatgactcgt gggaaagcat acggattgat gtatttattt
     7201 ttaattttag gaatttttgt tcctttccaa gtaattatga ttccgattac cgttatgatg
     7261 agtaaaattg gtttagcaaa tatgtgggga ttaatcttac tctatctcac ttatgccgta
     7321 ccacagacac tatttttata tgttggtttt attaaactaa atgtgcctat cagtctggat
     7381 gaggctgctg aaattgatgg ggctagtcac tttacaactt atttcaaaat tgttttccca
     7441 atgttaaaac caatgcatgc gacgactatt attcttaatg ctctatggtt ttggaatgat
     7501 ttcatgctac cattattaat tttaaatcgt gattctaata tgtggacact accattgttt
     7561 caatataatt atacgggaca atattttaac gactatggtc caagttttgc ttcctataca
     7621 gtaggaattg tcacgattac actagtttat cttgctttcc aaagaagtat tatagctgga
     7681 atgagcaatg gagcagtgaa ataaccaata aattattctc attataaagg agttcataaa
     7741 cttatgatta tatttgacaa tcacacacaa acatttcatt tacaaaataa aaaaattagc
     7801 tatctattag gaatcgaaga aaataattat ttaacgcatc tttattttgg aaaaaagatt
     7861 acgcactatt ctggtgggta tcgctacccc cgaacagatc gttccttctc acccaacaat
     7921 gcaaatgcgc aagatcgttt attatcttta gacacattac cattagaatt tccaggatat
     7981 ggacatggtg attttagaga accggcgcat aatattaaat taggaaacgg tagtcgtgta
     8041 aatgattttc gttatgatag ctacgaaatt caaaaaggaa agcccagctt agcgggattg
     8101 ccagccttat atacggaaga tgatgatgaa gctgaaacgc tgattattca attgaaagac
     8161 gcgattagtg gattgacttt acagttgttc tacacaattt atcaaaatca cagtgtaatt
     8221 attcgttcta cgcaattagt aaataatagt gatgaaacag tggagattaa ccgcttagct
     8281 agtcaatcaa ttgattttcc aaatcgtcca ctcgagttaa ttcatttaaa tggtacctgg
     8341 gcaagagaac gtcaaatgac tagagaaaaa attcatacag ggataaaagt attagatagt
     8401 aaacgcggat caagcagcca tcaacaaaat ccgtttgttg ctttagtaga tgccaaaaca
     8461 gatgaatttc aaggagaggc atatggattt aatttagttt atagcggtaa tcacgaaacg
     8521 gtgattcaaa aagatccatt tgaccaaaca cgggtcatta tgggaattaa ttcatttaat
     8581 tttggttggc aactaaaaac tggcgactca tttcaaacgc cagaagtcgt gatggtttat
     8641 agtaatgaag gtctgaatac gttatcacag acgtatcatg aattgtataa tcaccgttta
     8701 attcgtggaa actatcgttt aaaagaacgt cccacgttaa tcaacaactg ggaagcaacg
     8761 tattttgatt ttaacgaaga aaaaatcatg aatattgtcg atgatgcgaa gaacttgggc
     8821 attgaaatgt ttgttttaga tgatggttgg tttggcggtc gtgaaaatga cttcacttca
     8881 ttaggtgatt gggttgaaac agaaggcaaa cttgcttgtg gtttggaaaa tttagccaag
     8941 caagtgcatg ataacgggat gaaatttggg atttggtttg aaccggaaat gatttctgaa
     9001 gatagtgaac tatttcgagc acatccagat tgggcattgg gtattccagg tcgtgaccgc
     9061 tcaattagtc gaaatcaata tgttcttgat ttttcaagac aagatgttcg tgataatatc
     9121 tatcaacaaa tgacggcgat ttttgatcgt gtaccgattg attatgtgaa gtgggatatg
     9181 aatcgaaata tgacagaagt ctattctgtc ttgttggact caacgatgca aggtgaagtg
     9241 gctcatcgtt atatgttagg gttatatgaa tttttagaaa agttaacaac gaattatcca
     9301 actattttat ttgaaagttg ttcgggtgga ggcggtcgtt ttgatgcagg tttcttgtat
     9361 tacatgccac aaacttggac gagcgacaat accgatgccg tggctcgtct gaaaattcaa
     9421 tatggaacca gcttagtgta tccaatttca agtatgggtg cgcatgtttc agcagtccca
     9481 aatcatcaaa caggaagaga aacgagttta actattcgtg gcaatgctgc gatggctggt
     9541 gtttttggtt atgagctaga tctaacgcaa ttaacttctg aagaaaaagc agaaattaaa
     9601 gaacaagttg ccttttataa aaagcatcgc caacttttgc aatatggaac tttttatcgc
     9661 ttggaaagtc cgtttgaagg caatgatact gcatggatgt ttgtctcaaa agaccaaaca
     9721 gaagccatgg tcttttattt ccgagtatta gcagaagctg cgcatccatt agtcacattg
     9781 aaattagcag gattagcaga agaagcaact tatcaaatgg aagatgtaac atttgctggc
     9841 gatgaattga tgaatctcgg tttttatatt gactcagaac tacatggtga ttatgcgaca
     9901 caacgctttt atttgaaaca gatatagtag cttgaattta caatataagt gcaacaccaa
     9961 aagagactca ttccaaacga agaaaatatg ttttatttac acgcatttac taaaaagcag
    10021 cctgatttga attgggaaaa tgaagaagta cgtgaagaaa tctatgcaat gattaactat
    10081 tggttagata aaggactagg cggatttaga atcgatgcga tattaaacat caaaaaaaaa
    10141 ctagagtatg gcatctttga atctgatgga gaagatgggt tagccttcat tgggccctgg
    10201 attcttaatc agtcgggcat tgaagtgtgg ttaaaagaaa tgagagagcg gacatttaaa
    10261 ccacataata gtatgacggt agcagaagcc gatgttccta atgaacgttt agatgaatat
    10321 attggtgaag atggctatta ttcgatggtt tttgatttta gttatacgga tattgatgta
    10381 cctgaaactg gtgaatggtt taaagatagc caatggacat ggacggatat gcgtaccaat
    10441 atttttacga atcaattagt tacgcaagat aaaggttggg gcgccttata tttagaaaac
    10501 cacgatcaac cacgttcaat taataaatat attccagaag aatggatcaa tgattatagt
    10561 aaaaaaatgt tggcgacgct ttttatgtta ctaagaggta ccccttttat ttatcaagga
    10621 caagaactag gaatgaccaa catagaaatg gcatcattag aagattttga tgatgtagct
    10681 acgcattctc agtataaacg ggcattagaa tatggattgg caccagagca agcattgaaa
    10741 gctgtcaata aacgaagtcg ggataattct cgcacaccta tgcaatggaa tacgcaaaaa
    10801 aatgccagat tctcaacttc tgataatgta tggttaaaag taaatcctaa ttatccagaa
    10861 ctcaatgcag aagctcagtt aaccaatcaa cagtcggtat tcaattatta tcatcaatta
    10921 attaaattaa gaaaaaatag tgtatacaaa gacgtgctaa tctacggaca atttctacca
    10981 gtgaaagata aaaatgaaca tattttgctt tatgaacgcc aattagaaga taaaatcgta
    11041 ttagttatct taaacgttac agcaacagaa caagaatatt atgtagatga acagtaccaa
    11101 caagtattaa tcaataatta tgaagatgtt ttattagatc aaagtcgaaa attgcgttta
    11161 cgaccgtttg aaagtattgt attagctaat tatggaggac aattatgaaa attaaaaacg
    11221 aagcaatgct catcacctat gcagatagtt taggaaaaaa catgggcgaa ttaaaacaag
    11281 tcttagacaa acatttacaa ggtgttattg gtggtgtaca tctgttgcct ttttttccat
    11341 cgacaggtga tcgaggcttt gcgccgagtg attatacgac agttgatcca gctcttggaa
    11401 catgggaaga tgtggaagca ttaggtgaag attattattt gatgtttgat tttatgatta
    11461 atcacatttc tcgtgaatca aaatttttcc aagatttcaa aaaaaaccat gagcagtcac
    11521 catacaaaga aatgtttatc cgtattcatg aatttttccc agaaaatcga ccaacacaag
    11581 aagatattga tttaatctac aagcgtaaag acaaagcgcc gtttcaaacg gtagaatttg
    11641 cggatggtac gacagaagag gtttggaata cctttgggga agaacaaatt gatttagatg
    11701 tgaccaaaga agttgtgaaa gaatttattc gagaaaccat caaagatatg gcgaaccatg
    11761 gctgttcttt aattcgatta gatgcgtttg cttatgcgat taaaaaatta gataccaatg
    11821 atttcttcgt ggaaccagat atttgggaac ttttagacga agtgcgtcaa gaagcagcga
    11881 aatatgacgt cgaattatta ccagaaatcc atgagcatta ttccattcaa atgaaaattg
    11941 ccaatcatga ttattatatc tatgacttcg ccttgccaat ggtcactctg tattcacttt
    12001 atagtggaaa atcagaacgt ttagccaatt ggttgaaaat gagtccgatg aagcagttta
    12061 caacattgga tacgcatgat ggaattggtg ttgtggacgc tcgtgattta ttaacagatg
    12121 aagaattgga ttacacctct gaggaattgt ataaagtcgg ggcgaatgtg aagaaggtct
    12181 attcaagtgc caattacaat aatttagata tttatcaaat taacagcacc tattatagtg
    12241 cattaggcga taatgaccgt agctatctgt tagcacgtgc gattcaagta tttgcgccag
    12301 ggattccaca aatttattac gttgggttat tagcaggaaa aaatgatatt gaactgttgg
    12361 aagcaacgaa agaagggcgt aatattaatc gtcattacta tgatttagcc gaaattgaag
    12421 aagaagtgca acggccagtg attcaacaat tatttgcact tttaaaattt agaaatgaat
    12481 ctgcggcgtt tgatttggat ggaacgattg aagtagcaac gccatcagaa tcagaaatag
    12541 tgattacacg taaaaataaa aacggtagtg atagtgcaac attaacggct aacttggata
    12601 cgaaagaatt tatcattgag tctaaagata taaaaatttt cactagtgtt gcattaaaat
    12661 tttaacctat tccatagaac aaaaaatcct ttatactagc acttggatga cttatccaag
    12721 accaataaaa aggactcata catggataag tataaaacaa atacagcatt taataaatgg
    12781 ttttctgcca taaaattagg aaaactttct gtcaactccc aaaaatgtat ttttgattac
    12841 aattcgtatc gtaaaaaact gtcttttgaa gctgtgctca agctctttct ctacgcagtt
    12901 aatgaagaaa aagaaagttt gcgtgatttg agtacagctc ttatcgaccg atcgctccaa
    12961 ctagaaaccg atgtgtcatc gattagtcat acccaattgt ctcgagcatt taatgcactt
    13021 gaacctaaag tgttagaaga aatttttcaa caactgttag aaaaagttca acatcaaatc
    13081 aaaccaacca agcgaaatca tctgtatctg attgattcca gtacgttttc gcttagttta
    13141 aaacgtcata agtgggcgac tttccgtcaa acaaaatcag gtgtcaagtt acacttaaac
    13201 tattgttata tggacgatac tacgatgtat ccaactgatt ttaccttaac aaatgcccat
    13261 gaacatgatc tcaatcaact accagtattg gtgaatcaac cagaggcaac ttacgtgttt
    13321 gatcgtgggt attaggactt tgaaaagatg gatcagatgc attgggatgg ctatttcttc
    13381 gtcactcgga ttaaaaaaaa caccaaagtt cacgtgatag atactctgga aacatcgcca
    13441 gaaacagaaa ttctacgaga cgagctggtt cgtttaggat ctaaaaccta tctaaccgcc
    13501 aatttccgac tagtcactgt tcaagataag aacggacgtg tgtttcaatt tatcaccaat
    13561 cgaatggacg tctcttcaaa agaaatctct gatatgtatc acgctcgttg gcaaatcgaa
    13621 ttgtttttca agcacatcaa acaacacatg accattaaaa ccttcttttc tcaaagtgaa
    13681 aaaggcgtgc agaatcaatt gattttaacg atgatttccg ctctcctcac gtttctcatc
    13741 aaattggaga cgaaaacgga aaaatcagtg ttccaaatca aacgattctt tcgttattta
    13801 ctgtttcagc cctttgaatg ttgtctagaa aaattaattc ccacatgaga aaaaagaaat
    13861 ttggctcgtt acttttggtt ttcagttgaa taacagataa aaaattttct ggaaaaagtc
    13921 atcctctgcg tcagcacagt tttttttgac ttattttcag aggaaatagt tttcagaata
    13981 ttctaaaatg ttttatgcaa cactagtgat attttttaga attagatttc taatgtgcat
    14041 tttttgagta ttaaggagca agtatacaaa gatttataaa gctgattaaa tttgaataaa
    14101 aatatgcagg aacgctacta tttttagtag tgttcttttt tatatttgaa taaaaaatgt
    14161 ggtggttaaa tggtggttat aacaaaaaat gaattaatga aattaggtta tgggaagtat
    14221 cagtctgaga gaatcatacg acaggcaaaa gcaattatgg tttcaaaaag ctatacctat
    14281 tacgaaaata gaagattagg actagtacct gttgaatctg tagaagaagt tttaggtatg
    14341 aagatagagg tagaggagga ttttcagaat catggttaat tttaaaaaag ataaaaatgg
    14401 gaaatatcat tttactgcta gtttgggatt cgacaaacga acagggaaaa gagtacaaaa
    14461 aaacgtagag gggttaaaac agttaaagaa gaaaaaaagc gtataccgaa cttgtttcta
    14521 attatggaaa agaagcagaa acttctcaaa gtactatgtt gtttgaggag tttttttata
    14581 aattattcct accttattat caagggaaag tagaagaaag aacatataaa aatcggttat
    14641 ctacaatcaa aaaaactttt cttatttctt taaaatggag ttaagagaaa tacgtccaat
    14701 tgacattcaa aagtggcaaa ataatttatt gagcaaatat aaaaattctt atgtaagaaa
    14761 catgtatggg atttttcaga tgtcattaga tcgagcagtt gttttgggac tacttgaaaa
    14821 aaatcctgca aaagttattg gaaatgtgaa gaaaaggaaa actacagttg atttttggac
    14881 aaaagaagaa tttacaaaag ttatttcaac tttttatatt gatgatttgt accaacattt
    14941 tggttttatt tgtatgtggt tactttttat gaccgttatg cgtttgggag aagccactgc
    15001 attaacttgg aaagatattg attttgaaaa taaaaacttg cgtgtgaata aaacactcta
    15061 ttataagaga gccaaaacct atgagtttgt agaacccgaa acggcttcta gtattcgtac
    15121 aatcgaacta gacgacaata caattacctt tttaaaagct tggaaggagc gtcaagtttc
    15181 aataggaggc atgtcgtttg tactttctta taatggaaca cctactcaga aatatacaat
    15241 ccgtcatatt atgagaagac atgcaacgat cgttggtgtt catataatcc gcatacacgg
    15301 attaaggcat agtcatgctt ctttgcttat atcacaataa aaagatgcac ttcgaattaa
    15361 agaaaggtta aaacatgctg atgtacaaac gactttagga acttatggtc atttgtatct
    15421 aaattcattg agagagataa caaatgatcc cacagattat atccaaacta atttttctaa
    15481 aatcaaaccg acacctattt acgataataa aaaataaatg ataaacaaaa gtaggatcac
    15541 accaaaagaa acacactaga aaaactataa tacaggcttt tctagcgtgt tgtttcttat
    15601 ttccgcctat ttatacatta tactcataaa gcatttaatg catcgatcgt agccgcattg
    15661 tctatatggg tatataaagt tgttgtattg gtacttgaat gtcccaattg ttgggcagtt
    15721 aataccaatg aatttgtctg ttggtagagt ctagtagcta atgtatgacg cagtttgtga
    15781 gggctgactt gaattctaaa gccttttgaa tattttgcta ccatccgttc gattgcaata
    15841 ccggatatac gcttggcttc tccttggtaa attgctagaa ataaagcttt ttcgttttct
    15901 gttactttat atcgttcttt ccgaatagct agataattag ctaaatattc cattgcgaat
    15961 gctgctatat tgacagcatc tcttttattt ccttttctag ttactaccac attattctta
    16021 tctaaagata ggtcatctaa attaatattt ataacctcgg agagtctcaa tccagatcct
    16081 aaaatcagtg cattgatcgc caggtctctt tctacatctc gttgataata gatcaaagct
    16141 cgtttactca aagtattttt atattcattg gcaatataat ctagataatt ttgtgtatcc
    16201 ttatctagaa ataatttttt ctcaatggca ttggcacgtg cagctaacgt agttttatct
    16261 gtgatggttg ctacttgatc cataggatta ttgtctaagt atggttttcc tagaggatta
    16321 ttggaacttt tcgaaagata tttaaataga acctttaatg ccgtgatatt tctattgata
    16381 gtagaagtag atagacctaa gttattatta tctttgagct tagtttttgt tgtctctttt
    16441 tttacatttt cttttgtgcg acgtttcaaa tgagatttgt acaagtttag gtcataggca
    16501 gttaactgtc ttatcgaggt gatagaagta tcaggaacga tagactcact cagaagccat
    16561 tcataaaaga gcttataatt ttttagatat tctaaagtgg ttcgaggtga ttgatcttta
    16621 tcctcacgat cttgaacaaa atcttttata taatcgggca tatgaagtat gattgtatct
    16681 attttttgat atatttctgt atttttcatg tcattcatcc tttcaataag taggggagaa
    16741 gagtatctct ataattggac tataccaatt atagaatgtt ttgtcaattg gtatgctcta
    16801 aaatttatta atttgtttca aaaaaagcat ataaacaggt atacgtttaa taa
//
LOCUS       DAPCMT010000050        16820 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPCMT010000050 DAPCMT010000000
VERSION     DAPCMT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.050"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..456)
                     /locus_tag="RD743_001400"
     CDS             complement(<1..456)
                     /locus_tag="RD743_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDU0563221.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="RD743_001401"
     CDS             complement(561..1424)
                     /locus_tag="RD743_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDU0563222.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="RD743_001402"
     CDS             complement(1620..3719)
                     /locus_tag="RD743_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDU0563223.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="RD743_001403"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="RD743_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDU0563224.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="RD743_001404"
     CDS             complement(5072..6913)
                     /locus_tag="RD743_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDU0563225.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9410)
                     /locus_tag="RD743_001405"
     CDS             complement(7095..9410)
                     /locus_tag="RD743_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDU0563226.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="RD743_001406"
     CDS             complement(9595..10455)
                     /locus_tag="RD743_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDU0563227.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="RD743_001407"
     CDS             complement(10514..11035)
                     /locus_tag="RD743_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDU0563228.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11313..12074
                     /locus_tag="RD743_001408"
     CDS             11313..12074
                     /locus_tag="RD743_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0563229.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="RD743_001409"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="RD743_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDU0563230.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="RD743_001410"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="RD743_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDU0563231.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="RD743_001411"
     CDS             16434..16790
                     /locus_tag="RD743_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDU0563232.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3490 c         2946 g         5767 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa
//
LOCUS       DAPCMT010000051        16535 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPCMT010000051 DAPCMT010000000
VERSION     DAPCMT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.051"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            98..1270
                     /locus_tag="RD743_001412"
     CDS             98..1270
                     /locus_tag="RD743_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDU0563233.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1257..1961
                     /locus_tag="RD743_001413"
     CDS             1257..1961
                     /locus_tag="RD743_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDU0563234.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            1966..3126
                     /locus_tag="RD743_001414"
     CDS             1966..3126
                     /locus_tag="RD743_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDU0563235.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3202..3480
                     /locus_tag="RD743_001415"
     CDS             3202..3480
                     /locus_tag="RD743_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDU0563236.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3461..3832
                     /locus_tag="RD743_001416"
     CDS             3461..3832
                     /locus_tag="RD743_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDU0563237.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            3848..4252
                     /locus_tag="RD743_001417"
     CDS             3848..4252
                     /locus_tag="RD743_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563238.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4249..4659
                     /locus_tag="RD743_001418"
     CDS             4249..4659
                     /locus_tag="RD743_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563239.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            4720..5307
                     /locus_tag="RD743_001419"
     CDS             4720..5307
                     /locus_tag="RD743_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDU0563240.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5380..5847
                     /locus_tag="RD743_001420"
     CDS             5380..5847
                     /locus_tag="RD743_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDU0563241.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            5893..6201
                     /locus_tag="RD743_001421"
     CDS             5893..6201
                     /locus_tag="RD743_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563242.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6396..13130
                     /locus_tag="RD743_001422"
     CDS             6396..13130
                     /locus_tag="RD743_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDU0563243.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13134..14006
                     /locus_tag="RD743_001423"
     CDS             13134..14006
                     /locus_tag="RD743_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDU0563244.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            14003..15052
                     /locus_tag="RD743_001424"
     CDS             14003..15052
                     /locus_tag="RD743_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563245.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            15056..15880
                     /locus_tag="RD743_001425"
     CDS             15056..15880
                     /locus_tag="RD743_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563246.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            15898..>16535
                     /locus_tag="RD743_001426"
     CDS             15898..>16535
                     /locus_tag="RD743_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDU0563247.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         5911 a         2543 c         3530 g         4551 t
ORIGIN      
        1 aaaatagcta attttgaaag tttatataaa ctctttaatg aaactaagct ctagaaatag
       61 ggctttttta tttttctttg aaaggtggtg aaaacatgtg agcttatttg atatctttaa
      121 gcagtcaata agaaatgaag agccttctga gtggataccg gattttgtat atggagagga
      181 agtatctgct cgggcatacc tcaaaattat ggcaaaaaat actgttttag attttgttgc
      241 aagaactatg tcaacgttag aagttaagtt taaaaataaa gatgggacat cagactggga
      301 ttatattcta aatgttcgac ctaattctga tatgtcagca gctacatttt ggcaaaaatt
      361 tttttatcgg ctcttagatg acaacgaagt gttagtaata tttacagatg ataatcaatt
      421 gctgattgct gatgatttta gtcgaacgga gtatgccgtg tatgatgatg tttttacaaa
      481 tgtgatggta aaaaattttg tctttgaacg ttcatggaac atgtctgacg tcatttatat
      541 ggaatataat aacgaagaat tagaaaggtt tacacgaggt ttatttgaag attacgccgc
      601 gttgttcgga cggattctag aagttgcaat gcgtaacaac caaattcgtg ggtctgtatc
      661 cattgattca acgggaacgg cgaatgatga aaaaggaaaa gatggtaaga ctagatctca
      721 aagattacaa gaatatattg ataggatttg gaactctttt aaaactaact cagttgcaat
      781 tgttcccaaa gtgaaaggct ttgagtatga ggaatacacc aataaacaag gcgtatcgaa
      841 tcaatcgcta gatgaactga ataagatgaa gtcgtcttta attgatgatg ttgccaacgc
      901 cataggagta cctacggcgc ttatttatgg tgaaaaagct gaactggatt caaatattaa
      961 agcttttcgt aaattgtgta ttgctccact agttaaaaaa cttcaagacg aattggtggc
     1021 aaaaataatt agtcgtcaag agtacaaaaa cggggaacgt atagaagtta aaaaagttct
     1081 ccaaaaagat ctattagagt atgctactca aattgataaa ataacatctg gtggtgcgtt
     1141 ttatattgac gaagttcgtg atgaagttga ttatgatgag ttgccaaata atgaaggaaa
     1201 aattcgtatt cgtacgaaaa actatgaaga aactgtgaag ggaggtgaga acgacaatgc
     1261 cgaaagctaa aaaagtacca tttcagttta ctaacgaaat tcagaatggt aagcacattc
     1321 tcaccttgag tggaaatgta cagaaaaaat attggcgtga tgatgatgtc ataaacgcta
     1381 aagatattcg tgactctcta gatactgtaa ccgatgatat tgtaatcaag ttgaacagcc
     1441 caggcggtga tgtgtttgaa gggattgaga tttacaatta tttaaaagat cacccatcaa
     1501 atattactgt agaagttaca gggcttgccg caagcgcagc aacgtttatt gtcgctggtg
     1561 ccaatgatgt gattatgaat gttgggactt ctttgatgat tcatgaagct tctacatttg
     1621 cttgggggaa taaacaggac atccaaaaaa cgttaaacgc attggaaacg atcgatgatt
     1681 ctatcttagc tatttattca gataagactg gccaatctgt tgataagttg cgtgaatgga
     1741 tgaatgaaga aaaatggttt acagcagatg aagctgtcaa atatggattt gccaattctg
     1801 tgaagcgtga agaaccacaa aatgaacctc aaaatattgc agcgatgatt caagatgctg
     1861 ttgccgttgc aatagcgaat ttaaaggaac ctgttactaa taaaatcgaa caagaatcaa
     1921 aacaaaaatc attaatcgca cgattgcgaa aaggagaata aagatatgtt aaaaattaca
     1981 gataaaactg cagatgcaaa aaaggcattt aatgctattt cttcgaaaga agatgcaaca
     2041 cccgaacaaa ttaatgctgc tctagaagaa tatgtaaccg ccattgcaga agatgccggc
     2101 aaacaggtgc gtgctgaata tgaagaattg aaaaatgtga cggacaaccg tgtattagag
     2161 gctcgtggaa ttcctacact aacagcagaa gaaacaaagt tttataatga agccgtaaaa
     2221 tctggaggtt tcgattctga cgtagtttgg ccagaaacga ttttggaacg tgtttttgaa
     2281 aatcttcaaa acgatcatcc tattttaggc ctcattaact ttacaccaac tgtaggtcgt
     2341 gtcaaagtaa ttagagcacg tcgtaaaggt gttgctgttt ttggaccatt gcataaagac
     2401 cttgaaggcc aactagatgc tgaatttggt gcaactgaat ttgttcaact tgcgttgacc
     2461 gcatttttct taatttctaa tgatacctta gatttaggac cacgatggat tgaccgtttt
     2521 atcaatcttt ctttaagtga agcagtacgt gatgtttggg ctaaaaaaat tattacaggt
     2581 tctggtaaag atgagccaat tggattgctt aaagatctag atggtgcggt cacaagtggt
     2641 gtatatccag ataaagctag cgcagggaca ttaacttttg cgaaagagaa gattgttctt
     2701 gaacttgcag gggtgatgaa aaaattatct aaatacacat acaaagtaga tggtgacgat
     2761 gaaggtgaag tgaaataccg ttctgttgct ggacgagtgt atctaattgt taatcctgtt
     2821 gattactggg atatcgtagc tagagttaca tttgcaaatt taaacaatgt gtatgggact
     2881 acgatgccat ttatcgctgt tgatcatatc atcgaatctg tagacgtacc agaaagtaaa
     2941 tgtattgcat acgttgataa agagtatgaa gctactcaat caagagctga aaaagtctat
     3001 gtatataaag aaacttttgc aatgaaacgc gcaactttgt atgctgtcga catgcttggt
     3061 aatggctatc caacaaataa tgatgcagca caagtatatg acttggactt tacagcgggg
     3121 gagtaatgtc ccctgcaatt ggtgagataa caccgacgac tgatggggca gtcattaatc
     3181 tttcgtaaat ggggttagag tatgaacact gaagaattta taaaagaata taaggcccga
     3241 tttcgtattt tccatacttc cgaagatgag gatatcggga aacagttaga gagcgggttt
     3301 gccgatataa aatctctaat cggcagtttt gatccttcca cttatgagaa aggaaaagaa
     3361 ttagtttatg aacgtactcg gtatctacgg aacgaatcac tagagtactt ttacgacaat
     3421 tttcaaacaa tgattatgga tgcatcaatt gatctggcag gtgctggata tgccaattaa
     3481 atcaaattat aagaagccta aaattgtagc tggtgatcta aatacgccag ttactttttt
     3541 tgagtttcaa cctaataagg gtccagaacc tggtgatgaa gaaaaaaagg aactgtattt
     3601 ttgtacagca ctagtttaca acccatcaat gaaagatcgt gacattcttg gcacaaaaga
     3661 aggcgttacg ataaaaatac gggatcccca tcaatcttat attcctacta acaaacataa
     3721 agtaaggata ttagattatc gatatggaga taaagtttgg gaaataatcg atgtctcacc
     3781 ggatttggaa gaaaattcaa ttgtaaagat agtattagaa ttaattactt aaatttggtg
     3841 ataaatgatg agtaacgtaa agtttgaagg cgttcaagaa acaatccaag ctttagaaaa
     3901 aaagtatgga gaaaagaaaa ctaagacatt aacaaaaaaa gcaatcaatg ttggcgctga
     3961 aaaagtagaa aaacagttgc aaacggacat gaccgttttt aaagacaaag gttatacgat
     4021 cgctgaggtt gttcgcaaga acgctactta tagagattac aaggcagaag ctgaaatcgg
     4081 ttggaatggt ccacatcaac gatatagaat cattcatttg aatgaatggg gatatacacg
     4141 aaagggcaaa caaattagac cgagaggatt tggtgtaata acaaaatctc ttaagaattc
     4201 cgagctgttg tactttgatg ctgtaggaga ggaggttaaa aagagtttat gaaagatatg
     4261 cttaatatca tttacgaggc tctttgtttg aatgagtata ttcattcaat gacctacaac
     4321 gagaataccg agcaatatcg tattaaattt tatgagcaac ctgaaaccgc cgataaaaca
     4381 ggtccaatga tcacaattcg tcctgtagat acacctaacg aagcgtacca cggaagtaac
     4441 aaagagcttt ctgtggagca tatgatacag attgacgttg aatcgaaata tagagcaaca
     4501 tgcaagcaga ttcaatatga gataaaaaaa gagatgaaaa aactaggctt tggccaaatt
     4561 tccggtcaag ggttagatga atactttact gaaacaaacc gctttgtaga cgcgcgtcgt
     4621 tacgaaggaa atacaaaaat ttacgatacc gaatattaga aattaagaca cgaaaataac
     4681 gtgtcttttt ttattaccca aaattaggag gaaaatttta tgactttagt aggattcaaa
     4741 aaaatgacaa tcggggtctt tgacagcaca gggaagattc cagcagcaaa tttatatgta
     4801 attgaaggtg aacaaaacaa aggggcgact gtatctgctg aaatcagtgg gctatcaaaa
     4861 gaaccatcaa aagtttacgg atcgaacatc gcttactatg tctctcaaaa aggaacaggc
     4921 gatgtttcag ctacttttgg attgttagat ttaccaacag aagtaaatga caaaattctt
     4981 ggttacaaaa ccgatacaaa caaaatcagc ttcttgggag aagatactga gccaccatat
     5041 tgtgcaattt tgatggaatc ggaggatttg aacggcgata cagcaatgct tgcgatgttc
     5101 aaaggaaaat tcagtcgtga atctattaat cttaacacta cgacaaatga agctttcgaa
     5161 ccagaagcag aagaatatgt attttcagcg attgccaatg atgttgaagg tgatgctaaa
     5221 ggccaaacag tcgcaaaata tgttggagac gaagaagctt ctattactgg tttgaaagag
     5281 atggtatttc ctgcagcggg ggagtaacta gccccgttgt tggagcaatc actcccacta
     5341 ttaatggggc aacagttgaa ttaagttagg aggataacta tgccagatac atttaaaatc
     5401 tataaaaaaa atggtactga atttacaaaa gtcgcagagg gtgaaagtcc actttctatt
     5461 actggaattg cagcaaatac tcaagttgct aaaggagatt atcaagctac gcgtgtggtc
     5521 ggaggtcaag aatcagtaaa agtagatatt cctgcattca ccactcttcc aatttcagtt
     5581 actggtgtga cgttggataa aacgacagct gaagttgaac aaggcggaac cttaaaatta
     5641 actcctactg taacaccagc aaatgcaaca gacaaaacag gaagctggag cagttcgaat
     5701 acagctgtag ctacggttac tggaggcaac gttacagtaa aaacagacgc tgaagttggc
     5761 ggaactacag aagtttcatt tactactacg gatggcggta aagtagcaaa atgtactatt
     5821 acagttattg aaaaagcagc gggctagtct tcggactagc ttttttattt ggaaaataat
     5881 ggaggaatct aaatggcaaa agtaagaatt gaattgaaag aagaatcagg aaagaaagtc
     5941 tatgaaaaca tagacacaac agggaaagat tatcgaaaag ctttagaaac aatcaaaaag
     6001 ctaaatgaag acggagtaat gatttggaat cgtttagaca tttatttgga ttttgctgta
     6061 ggtatcttca gggatagcaa attgacagct gaacaaattc ttgatggctt accatctgag
     6121 aaggtaatga gcacattgga cgacatccta ggagaagtaa tggggattga aagtaatcca
     6181 gacccagacg caaaaaagta acacccgaag aagcagaaga aatgtactta aacctatgca
     6241 gagagttagt gaaagcggga tggtcactat ctgacataga agataattcc tttgaaatga
     6301 tgatgaaaat tgcgtgcact tcgcctaaga aagagaaaca aaaagaagtt gatcttaaag
     6361 acttcttgaa atccatgtag agaggaggta aacatatggc aaatggaaaa cctttaggca
     6421 atatgattat caaactagac ttagacagtt cagctttttc aaaaggtcta gccggagcta
     6481 aaaacgctgt taaccatcaa atgaaagcaa tgaaatcaca gatgcaagta atgaactctt
     6541 caggaaattc tctcggtgct ttacaagcaa aatacaatgg tttaggcgga gttctacaag
     6601 caaatgaaaa gcaagtagaa ttgctcacta aggcttataa ggatagtttt gactctaacg
     6661 gaaacgctac tgcttcgact gctaaatacg ctaatcaatt gaatcaagca acagctagat
     6721 cagcaagtta cgaatctcag atgaaaacaa ctgttggaca gattgctcgg atgaaagtgg
     6781 aaacggaagg gtttacgggg aaactaaagg ctaattctga tgtattgatt aattctggga
     6841 aaaaaattga agcctttggt aaaggagttt ctgggatagg atctgcccta actgttggag
     6901 taacagctcc aatagtggca ggcgcaactg ctgttactag tgcagctatt tcatgggaaa
     6961 gtgcttttgc aggtgtaaaa aagacaaatg atgaagttgt agactcgaat ggaaacgtag
     7021 tttattccta tagcgactta gagaaaggtc tgcgtaatct tgccacccaa ttacctggta
     7081 gccatcaaga aatagcaaat gtagccgaag cagccgggca attaggtata aagactgaaa
     7141 acgtagttag cttcactaaa acgatgatag acatgggtga atcgactaat atgtcggctg
     7201 aaactgcggc aacttcttta gcacgatttg ctaatattac ccaaatgtct caaaaagact
     7261 ttgataaatt aggatcagtc attgttgatc taggtaacaa ttttgctaca accgaatcag
     7321 aaatcactga aatgggattg cggttagcgg gagctggtaa acagattgga atgagccaag
     7381 gcgaaattct aggatttgct actgctctat cttctgttgg tgtcgaagct gaagcgggtg
     7441 ggtctgcctt ttcgaaggtc atgatccaga tgcaactagc tgtagaaaaa ggatttggcg
     7501 cttttgatca gctgaaacag atggcagaag aacaaggtgt tccgtgggtt aatttagtaa
     7561 atgctgtgag agacggtgga aaatcgttaa aagcagtatc agagcaaatg ggattcactt
     7621 catctgattt aaagaaaatg tataaagaag cggacaattc gaaaagttcg cttgaaaatt
     7681 ttgcgaatgt ggcaggtgtt acaagtgaac aattccagaa gttgttcaag agtgatcctt
     7741 cagaagcaat catcaagttc attcaagggc tgaaagattc agaaaaacat ggtacttcag
     7801 caatcaaagt tctggatgac atggatataa aagaagttcg actacgcgac agtttattga
     7861 gagctgcaaa tgctagtgac atattcaaag gagcaattga aaaaggaaac ggcgcgtgga
     7921 aacaaaatac agctttaaca aatgaagcta ataaaagata cgaaacaact gaatcaaagc
     7981 taaagatatt gaaaaacgaa gtgattgata cagcaatcga tcttggaggt ccatttgtag
     8041 acgcgttgcg tgatggtcta aatgtatcga aaccattgat aaaaaactta gggaatatgg
     8101 cgaaagcttt ctcagctatg agtgatgatc aacaaagaaa cattatcaaa tgggttggat
     8161 tagcagcagc agctggtcca gcgctgaagc ttttaggaac tggtacttca gtaataggta
     8221 aagttacttc aactactgga agattgacga aaagccttgt tgatttagca gcgaaagcag
     8281 cagaaaagaa ggctatggat gcctttgcat cgtcggtaac aacagctggt gctgcagctg
     8341 caaagacagc aggagctgga ggtattggcg ggttagtccc tatgatggga aaaacagcta
     8401 gtgctgcaag tactgctgcg ggcgccagcg gaataggcgc aatgacaacg tcactaggtt
     8461 tgttaggtcc tgctctactt ggtattgttg gcgttggtgg cgcactggca gttggctatg
     8521 gagcatggaa attatttggt gaagaagctt ggaattcctc gcaaagagta aagcaatggg
     8581 gaacagatgt cggtgaggag atagatagta ctcttgacgg agtacagaag aatattgaag
     8641 gtgcaaatgg tcaattcggt cttctcaaag agggttttac tgttgatgat gcaaataata
     8701 tggcggctaa tttcgcaacc gctggaacat ctttggaaac atctttaacg ggtagaataa
     8761 aggcattaga cgatgcacta aaagggctac ctcaatcagt tcaggatgcc atgaaagaag
     8821 tagctgaatc agaagaagaa accttagggc aatctctttc taaaataaaa gacaacaatg
     8881 aagaaataaa aaaaatcaga gaaagagccg caaatcagaa cagagacttg actgtttcag
     8941 aactacaaca gatacaggat ctaatgaaag gaacttctca agcgtatgtg gatacgctcg
     9001 atgtttctgc taaagacaga aaaaagatac tatcagccat gaatggtgat gttgcacaag
     9061 ctacagaaga agaagcgaag acgtggttac aatctttagc gaaacaaaaa gctgcaacta
     9121 ccgaaaatta tagacaaatg cttgaagaaa aaaaggcggc tttaaaggaa aagggagtaa
     9181 gcaaagaatt cattgatgca ttagaagatg aaatgaatga ttacatcaaa actacaaatc
     9241 aaggattcga tagccaaata gctgctataa caaataaata tccacaattg attaacgaag
     9301 tgagtttatc aaatggtcaa ttatttagca aaacggcagc tgctatggac aatactggtc
     9361 agtatacaaa agtttttctc gaaagtaatg aaaagatttt agcccaagcg aagaaaacat
     9421 cggatgaaat agcagataat gccgaaaaaa atgcagaaca acttggtatg gtagctgatg
     9481 aggctacgca tgctggatca acttggaatg gcttagtttt agacgaaaaa acaggaaaag
     9541 taaaaactaa tgtacaagaa gtagtgaatg aagcaacgaa ggatttcact aaatggaatg
     9601 aattgaaacc tgttgttcat gatgcgaaac taacaacgaa tgcaaaagat gtcatttcca
     9661 tagcggcagt acaaaatggc tattgggatg atatggaatg gaaagaaaaa gaagcattat
     9721 tagaagatga gtgttctaaa aatgtataca aggctttaga aaatgctggt aaatggaatg
     9781 agttagatat tccttctaaa gaagcaatcc taacttcaaa tactcctgaa gttatgggtg
     9841 aaactctcat gaatttggga ttatgggata cttacaaact tcaagtgaaa gatttagatt
     9901 tgaatgcgta taaagtatac gatgctatcg atggatcaga agaaaaatta aatagttggg
     9961 ctcaattaga taaccccaca aaagaattgt tattagataa ctcagatttt tctacaaaac
    10021 tttttaattc cgaagaaatg tttaaacgtt ggaatcagtt atcatctaat gcaaagtttc
    10081 tttcggctaa taatactaaa tttacagagg ttcttttatc atctgacaga atgtggaatc
    10141 aatgggtaaa ccttcctgat aatgagaaaa gattacttgc agataacgaa gatttagcta
    10201 ccactgtttt atcttcagaa gaaatatacg gaagatggac tcaattacca gataatcaaa
    10261 aatatatgtt agctgacaat actgacttag caaacaaaat attcgcttca gaagaatcat
    10321 ggaatgcatg gaaagcaatt ccagacacag ttaaaaactt gcttggtaat aatattgatt
    10381 tactggctaa agtgaaagac gggactgtaa gtgtagaaga atataacaaa gttatgccac
    10441 ttcttaaaac tctatatgga gataacgtta atttaaaaga ggcaatcagt ggggctaaaa
    10501 gctctataga gaattacaac aaaaatattt ttcctagtga taaaactgca gtaggacacg
    10561 atgaggcttc acaagctgct aaagatgcat ttgaagcgtt caacatcttc caaacaaaaa
    10621 taccagataa aattactaaa actgtatcag ctgactttat aggtcctatg ccgaaaaatg
    10681 cgaaaggaac aaacttccat cctggcggag cagctatggt aaacgatcaa aaagggccta
    10741 cttatgaaga gcttataacc ttgccaagcg gagaaacttt tatacctaaa ggacgtaatg
    10801 tcgttttaga cttaccaaga ggttcgaaag tactgaacgc cactaaaact aaacgtctag
    10861 tgcctaaata tgctgatggt ataggaaaca taacgacggt ttcatctacg ctaaacttag
    10921 atgctttaat tttagctatt aacgagctaa cgacggtatt gagaactcaa ctgatggcag
    10981 gaacgactga tgctaaagat actaaactat cagatgaaac aaaagttgcc gatccgatta
    11041 ttccggacag tctttctgaa aaatcagatc agtatctagg aattggagct caatggttaa
    11101 ctaatctaat gaatggttgg aactcagcgg ttcctcaata tatgaatagt gaaatggttt
    11161 ttattacaaa ctatctcaat gcgctaaaac ttcaaaacaa tcctaattat acgcaaggag
    11221 caacatggaa taagaactta ctgaatggat ggaatagttt aactgggacc tttattgcta
    11281 caatcaattc attttgtaat caagcaatgg tcacgcttag aaactacaac actcctatgt
    11341 acaacaatgg ccgaacgtgg cagcaaaata atcttaatgg ttggaattca ttgtatggat
    11401 cattcatagc ccgtgtaaat cagcttggta acgattcgat taacaacctt cgttctaaaa
    11461 gtggtggttt ttacaatgca gggacattct tgttgcaatc tttaataaac ggaatgaatt
    11521 cgttaggtaa ttctctttct actacaatga acaatgtcgc taatacaatg gttggtggta
    11581 taggcaaagg agttaatggc gtcatatcgg gagttaacta tgtgcttaag gaagtcgaat
    11641 caagtaagaa tatcggaaac tggccaattc cacaatacgc taagggaact gatggacatc
    11701 ctggtggact tgctatgatt aatgatcaaa aaggcctagt tcacgaagaa tatgttcaga
    11761 tgccagatgg acgtagtttt attgccaaag gtagagatct tcttgttaat ttgcctaaag
    11821 gtgctcaggt cttaaatgct tctttaacaa agaaattaaa ggaacgttta aatattccac
    11881 actacgaaaa aggtattggc aatttagata ttgttgattt gttagatgat gaaaaaagaa
    11941 tgttagaatt cttaactagc aaagttgatt tttcaggcat taatgaacca tggcttgata
    12001 tgactaaatc aggaacgtca ttaatgtcca aagctgcaaa tacaatgctt caatcaaaat
    12061 taagtgaatt cttcactcat ggcaattttg atggtgcagt aaatgccaat ggtgtttatc
    12121 aatatttagt tgatgtggca cagaaagtaa tgggtaagtt ccccggactt acagtaactt
    12181 ctggatatcg agcaggagat gcctactatc acggaaaacg gcaagctatc gacttggctt
    12241 atccaggtat ttctggagat ccgagatata cagcagcagc aaattacgct tttgaaaaat
    12301 tcccttcaaa aattgcgtat gtcattacga atggacgtgt acgtgaccgc atgggattat
    12361 ctggaacaag gtcaagcgga caatggacga attggccaga tggcgatcac ttcgatcata
    12421 ttcatttaaa cggttcgatg gggtctggcg atatattcaa tggaggagtt ggaggaagtg
    12481 gtgttgcacg ctggcgttct tatgtgtcta aagcattgaa aatgaatgga ttaccagcta
    12541 cagcagcgta tataaatgcc tggatgtccc aaatccaaac ggaatctggt ggtaatgaga
    12601 aagcaatcgg cggcaatgat gggctcgccg aaggcaatgc aacaggatta cttcaaacga
    12661 aacccggaac ttttgcagct aacgcttttc ctggtcacgg aaatattatg aacggatttg
    12721 ataacatgtt agcagcaatc aattatgcta agaaacgtta cggagttgca ggtatgttgc
    12781 aagttatcgg gaaaggacat ggatatgcca atggcggctt gattaccaaa gacggactat
    12841 atcgagcagg agaaggaaat aaaccagaaa tggttattcc attgacaaga aaaacaagag
    12901 ctatcgaact tatgggtcaa gcattagcct tcctttctgg aagcaataaa aaagaaacga
    12961 aaagtgagat taattctgta gataatagac atatcctaca agcgatagaa aaacagtctc
    13021 gtatgacaaa tgatctgcta tcattattac tctcgttttt taaaagtaac aatcgatcag
    13081 ataaagaact agctttagat attcagaaaa ttttaatcag gaggacatag tatttgagga
    13141 cagtaatatt aagaaataaa ttgaatgaag aaattgattt gtccacagaa gattattttg
    13201 ctactgaaat gtcgaatcta ggttttgaag ttacaaaaga gcatattgga caatggggga
    13261 gtttcagaga aataggagag agtattgaat tgtctgaatt tcaatcatca gttatgattt
    13321 ctgttcacgg atttagagaa caacaattat atggttcatt agttgaattt ctggctaatg
    13381 gaccttttga actagaattt tcttttgatt cagaaacaat gatcaggaaa tgtagtttga
    13441 aatcattatc taaaacagaa attgattccc aaagctcatt gctaacagat actttgaatc
    13501 tatattttac ttcaagctgg tattctatta agcgtgaaaa attaatacag aattcaactt
    13561 ctctcaatac acgtggaaaa gtttactcat ttgttcgacc atatgtctac accaaaaatg
    13621 tatgggaaaa gaaaggtgta tttaagttca gtaataattc tgtatatttg acaaatagta
    13681 cagagcgaat gtctccgtta aaaataagga ttattggaga gtgctcaaat ccatattggg
    13741 aagttatcca aaactctaaa gtagtagctt cagatggcta ttttattaat atgaaagaca
    13801 cacaaatatt agaagtgtct agtttgctag aagaccaaac ggcaatttta aaagatgtag
    13861 gtggagttga atcttctgtt tatcaacaac aagattttac taaatcaaac ttcgtccaag
    13921 cgcctctagg agaattcagc gttgtttttc atgttggaca tgcaaatgta gagattgaac
    13981 tttatgagga gcgtgatcta ttttgatttt agccattacc ctctttcacc gagatatgaa
    14041 tttatataac gaacgtttaa tttcgactga gttcgaattt ggaacggatg aaattggaga
    14101 agaagtaagt aattttatta cagataaata tgtccatgta aaatcaggag attttctact
    14161 agcaaaattt attcctagcg ggaagattgc ttattttgga gtgattagct cgcaagaaga
    14221 caaaaaaata aattgcaaag gattactcag tttggccgat agcgagatac cgactgtaaa
    14281 aatatcaggt gctaattatg aagagcatat tcgacgactg attgaatact acttattgaa
    14341 cgatccaaca aaacaactaa aaaatatttt ggaggtaaaa acagaaagcg ttacttctca
    14401 ttcttatcaa gctgttgaca caaataagag acgattgaat gcatatataa taaatgggtt
    14461 caaaaaatat aatgttaaat ggtatgtcaa agagattaaa ggtaaaaaaa tatatactgg
    14521 tattcgagca gttgatgatt caatttatat taaagataat tcatctgagt ttaatgactg
    14581 ggatgttttt gtgcaatctc ctggaccagg gaacgagaac aaattattga tcgtagataa
    14641 agcaatgaaa gatatagaaa atcccttggt attgtctacg tggtatttag atgacgaaaa
    14701 taatcttacg caagattcaa cgaataagaa cattgcattg cctaccgtta atttagtaaa
    14761 tatatatgat caaactcaag aagatagacc ttcttatgaa gaggtagcta aatcggagct
    14821 taaaggcaac gcctattcac atgaaattaa agttaatgtt gtgctaaatg ctagaaatat
    14881 caatgtagaa aatatcgaaa caggaatgtt agcgactatt gtttacaaag agcagactta
    14941 caaatctgtt ctaactgcct ggcgaatatc cagcagtaaa aagaatatag aattgacatt
    15001 tggtaatgtc cgaagtcgtt ttatggatta ttttgaagat aatgggggat aaaaaatggt
    15061 tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta agcgcagaaa atgatgctag
    15121 actttatcac gttttatata atcggaaaaa tcaggttatt gatggttacg atcagtctat
    15181 gaatttatct tcaagcggat taacagtaaa agttgctgca ggagcagcga ttattcaagg
    15241 tcgtatggtc gttgttcgac aagaagaaag tataacagtt ccagcaaact caagtggtta
    15301 tatagcatta acagtggatt tgacacaaga agttatacct ggatctattc ttccagaatc
    15361 ggaagaatat gaatggacta ataatcaagt caagctagaa tttataacaa aagttataaa
    15421 aggtaattta aacaatggtg ataaggtcta taatttacca ctatgctcag ttaattctac
    15481 tggatcaact gtttcaatct caaagatatc ggatagttac gagctgactc tctctaaagg
    15541 agaaattttg tggaggggga ctgcgttaat gcatgatact caaactgtcc aaccttcaaa
    15601 aaaaatttgg cagacagttt caggtttttt gttagtatgg ctcccatacg aaaacgggca
    15661 agcaattgag gatagatatg taactacgcc tttttataag gagcgtataa cttttactaa
    15721 tgttttagga gaaattgttt caggatttga tgactatcac aaaaagtggt ttagtaaacg
    15781 aataaactat aattcgaata ctaatatatt tacaggtgca gcaagcaatg caagtggaga
    15841 taacgcaaat atggtgctta ggtatatagt ttctttttag ttaggaggta tggaagagtg
    15901 gcaaatttag aaattaaatt atctgcaaat aagagacagc cttatctacg tcaccgtgtt
    15961 gttggtagag ttggtgatgg ggggctcaca acaatcaatg tacaacttct tgaggaagat
    16021 gaaattacac cttttgtaat taatctaaac ggtactttga aatttgtggg cgaagtttca
    16081 aacggtaact ataccgaggg agaaccagaa ataatcgatt cgactaatgg gttaattagt
    16141 tacacgttca ctaagtcaaa tttcagcacg agtcatcaat tcaaacaagc atattttgaa
    16201 tatgtagatc ctaacggcaa aaaagtaact tttcagaact tcatcataga cgtgttagaa
    16261 cgagtagata ttaattcgga gcaagcgaaa tactatattt cttcattgga aaaattacag
    16321 agtgaaatgc aaaccacttt caatcagttc attagtgata aacaggtcca atacgatcaa
    16381 atctactcga aatataacga attagtaaga ttgataaatg aatcagataa gcaagtaaat
    16441 gatcgtattg ataaggcaaa taatcgtatt gatcaaacca atcagcaaat cggcgatctc
    16501 ggcaagctga aaaagatgta cagtaacagc attga
//
LOCUS       DAPCMT010000052        16522 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPCMT010000052 DAPCMT010000000
VERSION     DAPCMT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.052"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..258)
                     /locus_tag="RD743_001427"
     CDS             complement(<1..258)
                     /locus_tag="RD743_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563248.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="RD743_001428"
     CDS             complement(274..924)
                     /locus_tag="RD743_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563249.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="RD743_001429"
     CDS             complement(935..2107)
                     /locus_tag="RD743_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDU0563250.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="RD743_001430"
     CDS             complement(2126..2998)
                     /locus_tag="RD743_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDU0563251.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="RD743_001431"
     CDS             complement(3020..3940)
                     /locus_tag="RD743_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563252.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="RD743_001432"
     CDS             complement(3940..4209)
                     /locus_tag="RD743_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563253.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="RD743_001433"
     CDS             complement(4206..4586)
                     /locus_tag="RD743_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDU0563254.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="RD743_001434"
     CDS             complement(5053..6516)
                     /locus_tag="RD743_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDU0563255.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="RD743_001435"
     CDS             complement(6580..9597)
                     /locus_tag="RD743_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDU0563256.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="RD743_001436"
     CDS             complement(9748..10587)
                     /locus_tag="RD743_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDU0563257.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="RD743_001437"
     CDS             10732..10977
                     /locus_tag="RD743_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563258.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="RD743_001438"
     CDS             11040..11462
                     /locus_tag="RD743_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563259.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="RD743_001439"
     CDS             complement(11556..11873)
                     /locus_tag="RD743_001439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563260.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="RD743_001440"
     CDS             complement(11900..12106)
                     /locus_tag="RD743_001440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563261.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="RD743_001441"
     CDS             complement(12087..12269)
                     /locus_tag="RD743_001441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563262.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="RD743_001442"
     CDS             complement(12283..12633)
                     /locus_tag="RD743_001442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563263.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="RD743_001443"
     CDS             complement(12659..12961)
                     /locus_tag="RD743_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563264.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="RD743_001444"
     CDS             complement(12964..13197)
                     /locus_tag="RD743_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563265.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="RD743_001445"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="RD743_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="RD743_001446"
     CDS             complement(14628..15743)
                     /locus_tag="RD743_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDU0563266.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="RD743_001447"
     CDS             complement(15749..16147)
                     /locus_tag="RD743_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDU0563267.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4382 a         3448 c         2575 g         6117 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt at
//
LOCUS       DAPCMT010000053        16501 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPCMT010000053 DAPCMT010000000
VERSION     DAPCMT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.053"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..546
                     /locus_tag="RD743_001448"
     CDS             <1..546
                     /locus_tag="RD743_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDU0563268.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="RD743_001449"
     CDS             735..1403
                     /locus_tag="RD743_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDU0563269.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1989)
                     /locus_tag="RD743_001450"
     CDS             complement(1603..1989)
                     /locus_tag="RD743_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563270.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1961..3343)
                     /locus_tag="RD743_001451"
     CDS             complement(1961..3343)
                     /locus_tag="RD743_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDU0563271.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3499..4686)
                     /locus_tag="RD743_001452"
     CDS             complement(3499..4686)
                     /locus_tag="RD743_001452"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDU0563272.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4693..5724)
                     /locus_tag="RD743_001453"
     CDS             complement(4693..5724)
                     /locus_tag="RD743_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDU0563273.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5740..7194)
                     /locus_tag="RD743_001454"
     CDS             complement(5740..7194)
                     /locus_tag="RD743_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0563274.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            7369..8274
                     /locus_tag="RD743_001455"
     CDS             7369..8274
                     /locus_tag="RD743_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDU0563275.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8365..9042
                     /locus_tag="RD743_001456"
     CDS             8365..9042
                     /locus_tag="RD743_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDU0563276.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9039..9209
                     /locus_tag="RD743_001457"
     CDS             9039..9209
                     /locus_tag="RD743_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563277.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9323..10219
                     /locus_tag="RD743_001458"
     CDS             9323..10219
                     /locus_tag="RD743_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDU0563278.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10274..11644)
                     /locus_tag="RD743_001459"
     CDS             complement(10274..11644)
                     /locus_tag="RD743_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0563279.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11882..12628
                     /locus_tag="RD743_001460"
     CDS             11882..12628
                     /locus_tag="RD743_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDU0563280.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12625..13464
                     /locus_tag="RD743_001461"
     CDS             12625..13464
                     /locus_tag="RD743_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDU0563281.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13465..14415
                     /locus_tag="RD743_001462"
     CDS             13465..14415
                     /locus_tag="RD743_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDU0563282.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14556..15308
                     /locus_tag="RD743_001463"
     CDS             14556..15308
                     /locus_tag="RD743_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563283.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15327..16478
                     /gene="queG"
                     /locus_tag="RD743_001464"
     CDS             15327..16478
                     /gene="queG"
                     /locus_tag="RD743_001464"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDU0563284.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5368 a         3091 c         3316 g         4726 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttctcttga aaatgccaaa catgttatcc
     1141 tagagaatgc tggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt tgataccgtt gactagttct tccatcgcaa aagcagtccc
     1921 ccattttaat ccaaatggcc gatctcgata ctcgttatag tcaatgaatt tttttgctgt
     1981 ccgtctcatc ttttccacct tctaatccac tcataccacc tgcatgcccg ccaacgagag
     2041 aactacgtgc gatggcgcgt cctccttgca agagactatg cgcatggatg attgattgaa
     2101 agccgtattt ttttctaatc gtatcgacaa caaaatcaac tttcgcttca tttatctgct
     2161 tttcaggatc ttcaaaaagg ttaagctgta aacctgtcgc gtatacgagg tcggaacagt
     2221 tcacgcctac acgccgaata tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag
     2281 catctgctag atctctgcta gcatttgtcg cttcgatttt catttgctgg tgaaacccgg
     2341 tcttgccatt tggatcaaga tagcctaaag agtatcctac ccataagctg actacttgtg
     2401 tttttgctgc agatcgtctc aagcgagtgc cgacttggtc ggccatttct ttgataacga
     2461 tctcgatttc ttctcgtctt gtataatctc tatctaggat ttgactattt ccgatacttt
     2521 ttgattttgg tgtatattct tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat
     2581 aaagctgagt accgatgacc cctagcttgc ttttcaagtg ataataatcc gcgtgagcta
     2641 aatcataaac agaatgtata cccatttgtt tcagacgttt tgctgttcgt ttgccgattc
     2701 cccaaaagtc agtgatatct gagattttcc ataacttttc tggtacatct tcataacgcc
     2761 attcagcaaa catttgcttt ccgtcttttg agccattgtc taaggctagt ttagcaagta
     2821 gcgggttatc gccaattcct atagtcgtgt agattccggt ctgctcaaac acatctttat
     2881 ggatcagttt agccatgtcg tacgcatttg ttttaccaaa gagtgccatg ctatttgtta
     2941 catcaaggaa gctttcatcg acggaataga catgatggtt cgcttcgtct gcatattttt
     3001 tatagataga attgatttct ctgttctttt ccatataata ggccatcctt ggggcaacaa
     3061 tatataaatc acttggatag gggaagggaa gatctctggc acggctgata ttggtgatgc
     3121 cgtaagcttt ttttgctgca ggactggaag ccaagattaa tccgctaccc cgtgtttctg
     3181 tcgaatcgga cggataggac atgactacta atttagttgt aaggggatca agatcctttt
     3241 caacgcactc tacggaggcg taaaaagatt tgcagtcaat acataaaata tctcttgaag
     3301 gttctttttc ataaacaaat accggatttt gattcgccca cataacaatc acctcttcaa
     3361 cacaattaga acacacgttc tctttccgtg tcaataaaaa acagcaattt tcaaatacga
     3421 gcaaaaggct cacatttgga aattgctgag aggttatttt tatctatttg tttttagctg
     3481 agatcgttga tttttttatc aaacatgaac agcttctgga tcgatctttg ttgtcccatt
     3541 tccaatgtgt gcaacataaa attccggagc atagccgaca actttcatgt attcatcata
     3601 gacattgttt ttgaaggcag gtatattttc ttctttgaca agagcaatcg cacagccacc
     3661 aaatccagct cctgtcattc gtgcacctaa gacaccttct tgtttttgag cagcatctac
     3721 tagtgtatct aattcgatcc cagttacttc ataatcatag cgaagagagt ggtgagaatc
     3781 atttaacagt tgaccaaatg cttctaagtt cccagcttct aattcagctt ttgccttcaa
     3841 agtccgctga ttttcagaaa cggcatggcg agctctgcgt atcaatgtat catcgccgat
     3901 ttggtcaata gttgattcaa attcttcttc atctaagtct cctaaagaag agataggcaa
     3961 agttttttgc aaacgtgcca aggcttcttc gcattcgcta cggcgttcat tgtattttga
     4021 atctgccaat tcccgccgct tattcgtatt catgatgacg attgcataac catctagttt
     4081 cactggaacc atttcgtatt tcagcgtatt tgtatctaat aagattgcat gatctagctc
     4141 accgaagccg atggcaaact gatccatgat acctgaattg acaccgatga attcattttc
     4201 tactttttta cctgtttgga ctagttccaa gcgatctgtt tttaaatcaa acagatcttc
     4261 taatacaaca cctactaata attctaatga agcgctactg gaaagtcctg caccattagg
     4321 gattgttcct tccactaaca actcaaaccc atgatcgatt gagtgtccag cttcttttaa
     4381 tttaaggacc attcctttta cgtaatttgc ccagtttgct tttttatcat attctagctc
     4441 atctaaagta aaactaatga caccgatatc gtcaaagttt gtggaataaa ctaatatttt
     4501 attatcgtct cgaggtgctg ctacaccttg cgttccataa gtgatggaag cggggaagac
     4561 atggcctcca ttgtaatctg tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta
     4621 ttgtgtagtt tttttaggtc cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat
     4681 ttccatgatg agttattcct ttcttgtttt gattttgaat tctgtcactg tttcaaatac
     4741 tgatccagct tttaaatgaa tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt
     4801 ttgagtctca aatgtgatgc caccgtgatg tgccagtttt cggttgcgca tttctggtgt
     4861 ttctgtgccg aaatttgcag tgaaaataac cacactagaa gcatctgtag caatacctac
     4921 ttggattctt ttatcggggc tagtcaagcg agcggcttct ttacccaagc cggtttcttt
     4981 taagaaaaat ggatgatcga ttccgtcaac aagatttttt tgatccaaat cactagcaaa
     5041 aacggacgct aattttttag gtatttgaaa atcgaaagca cttcctgcag cattttcttt
     5101 tacccctata ggaatactat ctgttcgcaa aggagcataa gcttcgctat tgagccaaag
     5161 ctcatgttga tcgattgact gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt
     5221 cggattgaat aaagtatctt ggtcactggt gccgcgagag gtcactcgcc agatattatc
     5281 ttttgtcaaa gtatagcgga tttccacatc cattgtacct ggaaaaccat gttctccgtc
     5341 gggacttgta gtagtaaaaa tgacagaagc ttcattttcg ccattcaaaa tgacataaga
     5401 ccattttttt aattcaaatc caggagcacc accatgcaga ttatgtccag tttttggatc
     5461 tgtggctaat tggtaagttt ttccattgag agaaaagcgg ccattttcga ttctccctgc
     5521 ggtgcgaccg atagaagcac cgatatacgg atctttttcg atatattctt cagcggtgtc
     5581 aaatcccaaa actaattctc gatcatttga ttttaaactg acgattctag cacctaaatc
     5641 agagatagaa agtgctagct tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc
     5701 aaaatctttt tctgtaatct tcattttcaa gatcctttct taatgggaag gaactactgc
     5761 atcgtgaagt tcatcttcca ttgtatcagc tggagccaat ttgttttcca attcctcaac
     5821 gatttgtgca tgttttttct ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa
     5881 aatcatcaaa gcagcaggag catagaatgc ataagctttg aaagtcgtta cgccatgagc
     5941 agtgatatca gcagcagtag cattaccagt catcccagaa gcgatagcga caaaccctac
     6001 gatcccgtta gacaaagcac cagcgatttt atctagtaaa gggcgaattg atagagtaac
     6061 agcttcgtta cggataccat ttttccattg accatattca acagagtctg tgatcgtcat
     6121 caaagcggat aagaaaatca attgttgagg gaagtagaac aatactagcc cgatgataac
     6181 gattggcaaa cttgaacctg cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat
     6241 ccctccaagg taaacgtagc gacgagagat gacgcgtgta agcattggga atagtggaac
     6301 agctactaaa ccaagtaagg cagcaactag accaacgatc caaaattcag ttggttggcc
     6361 aagtacatat ttgaagaagt agaataatac ggcagttgtt gctacattgg caatcgcata
     6421 agcgatataa gcaagggcaa gccacatcaa ctgatcattt tgagcaactg ctttgaacac
     6481 atctttgatc ccagcttttt cattttgctg acggatcgca gtatcgactt cttttgtgcc
     6541 caaggcagtg ataatcgcag taccgcctga aataacagca gcaatcacac cgaaccagaa
     6601 ccagcctgaa gcgccttcaa tatgtttatg agtaacaaga taggtaaaga aagtaacgat
     6661 agggacaacg actagagtag taccatttgc acctagagaa gagccgaaac gagcaaacgt
     6721 agcaaatttt tcacgttcct ttgtttccgt actcaaagct ggaatcattg accaaaaagc
     6781 gatatcctta aatgaataaa aactatccaa taagatgaaa acgattgcaa ataatatgat
     6841 aaataaagtc gtattatgag tagctaagcc aaagaaattg gtaaagattg ctaccaagag
     6901 tacggaacta acaacgccgc cgataactaa ccatggtttg aatttaccgt attttgtacg
     6961 agtattatcg atgatgccac caatcaaagg atcaaaaata atttcaacta agcggatacc
     7021 tacaactaag cttgtaactg tagcaatcat ttttgcattc gttgccttat ctgttcctgc
     7081 gaacatctgg cttgtcacga ataccataaa ataagtgctt aatgtaacat agaatgtgtc
     7141 gtgtccaaaa gcgcctaaag cgtaggacac acgttggatc attttgcttt tcataatctg
     7201 tattcctctc ctgttcaata tataatacta agtaaaccgg ttacaacgaa taacaaagat
     7261 cttttaatga cttaaactta acaagaaatc gcttcctttt taatatattt ctgtgacttt
     7321 tatttaactt tttgttgcat tatactaaag gagtaaggag ggatagacgt ggagcaatcg
     7381 atttttttaa aaaaggaaca tcaacaagta gaaaaagaat tgtattttga tttttgtggt
     7441 ttctctaaaa cgatgccgtt tcattctttt ggtcctgcta ttcgcaatga ttacatcctg
     7501 catgtcgtga tggaaggcaa aggatcttat catgtgaaaa atcaacaatt ccaattgaaa
     7561 aaaggtgatc tgttcttgat tcgtccagga gactcgacgt tttacttatc agatggagaa
     7621 gatccgtgga tgtattgctg gctttcattt ggtgggagtg ctgcagaaga aatcattcgg
     7681 cagtcgcttt tcagagaaga ccaatacacg atggtctctt ctgacataca aaaatatatc
     7741 gacattattt tagaatgcat gaaagatacg gaagaaacct tggaaaatga attacagtta
     7801 acagcattaa cctaccgttt tttatcagtc cttttaaaag atggcggaaa ggtcagcggt
     7861 ggtgtacaaa aggttcattc ctctttagct gttgaaacag tcaaatacat cgaggcacat
     7921 tatgctgaaa aattgtctgt tgaggaaatc gcccagcatt tagcggtcaa tcggagccat
     7981 ttgtcgcgag tattcaaaaa tcatatgggt accagtatca aggaatattt gatcggggta
     8041 cggatcaatc gaggggcgtt tcttctttct ctgacggatg aatccgtgga aagtattgcc
     8101 tatcaagtag ggttcaatag tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga
     8161 gaaacggcta cgaactatcg taaacgtatg aaaaacgaaa attttaagaa tattgctttg
     8221 gataccttga aaaaacagtt ggaaaaacaa accgcagttt cttggacaac ctgattagtc
     8281 tggttaaaat gacaagcatc ggcaaaagaa acttgtggcg gttatgctac actgtaaagc
     8341 gaagactaaa aaggaggaaa agggatgcgt acacttatca tcattagcca tccaacgatc
     8401 gaagaatctt taaatcagca atttttcaaa gaagcttctg ctggtttacc agctacttgg
     8461 catcatttgg aatcaagtta tccagacgga aaaatagata tagagactga actacagctc
     8521 ttggcccagc atgatcggat cattttccag tttccgtttt actggtatag ttctcctgct
     8581 catttaaaaa tttggcaaga taatgtcttg gaacgagcag cacatgtatt aaaagggaaa
     8641 gaactaggaa tcgtattgac aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa
     8701 gaagaatata cgatttccga atttttgagg ccgtatcagc gaatcgcaaa taaattccat
     8761 atgacctatc ttccgccatt cgttttagca caatttatgt atttatccca ggaaaaacgt
     8821 tgggagaagc taattgctta tcagcagtat ctctcattgg aaggcaaacc atcgctgact
     8881 cagcggattg actggtttat tcaaagagta caggaaaatc agaaaatgca ggaagaagaa
     8941 tctgaaaaac aagcatatat cgtagaagca ctgactgagg caaaagagca gatcgaagac
     9001 ttgaccttta cactgcaaga aatgaaagga acttcattat gacaaatgat tggctgatcc
     9061 aacaattaga tgaattaagc gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa
     9121 taaagaaact gatggctgag atagacgaac gaacaatgca gctgcaagga gaaatcgacg
     9181 gacaatcttg gaatcatctc aactggtgaa attttcttat aaataacaaa gataaaaatg
     9241 tgcacagcga caattatttg ctacaataag aatatgaagc gtttgataac gctattatac
     9301 ataaaaattg gtggtgaaaa aaatgtatga tgaaaatgag tggggagaaa atgttcggat
     9361 gacccgaaga agacagcaaa aacaggcagc tcgtaagcgg atgatcatga ttggggcagc
     9421 agttgccgtg tttttgatca tagcaggagg agttgcgggt ctatattttc atggaaaaaa
     9481 ttcgtcagaa gcgaaaagta cgaccataac ggaaacaacg acaaagaaaa cagagacgaa
     9541 acaaacaagt gagtcatcaa ctaaaacaaa agagacacaa acttctgaag aaaaaacagc
     9601 aaatggtttg ccagagcaac ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga
     9661 aaagacgcct gcattgattg aagaatacca gacagaaatt cagggaaatc aagagggaat
     9721 agctggttta tcagcagttg ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga
     9781 agggatcaga aaattacgtg cagcgtatca ggcagcagaa aataaagacg gtgtagactt
     9841 agatactttg atcaatcaat tatcggctaa ttatacgaat catgtcgcta agatttccga
     9901 tatttatttg cagactagcg cctctcttca agcagagtca acaagcacac aagacacaac
     9961 atcttctgat tctgagacag cagagtcggt agaagcttct gaaatgcaag ctcgaacaac
    10021 acaagacaca acagattcct ctgaaactaa taccaacagc gcgacttcac aggctgcgac
    10081 aaccgtagtt ttggaaggag aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt
    10141 agaaacgatc ttgtcgctga atgggatgag catggagaat tatttcctga ctcccggtga
    10201 gacgctaaaa ttgaattgat aaaagcaata aaaaagaacg aagcttccac taaggtgaag
    10261 cttcgttctt tttttatttt ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt
    10321 aaagcaagaa aacgagtcca ataatcaatg agattctcgt ttcaggattg ataaacatga
    10381 aaatcaatac gatagctaaa aaagcaagtg tcacataatt cgtataagga gatagtggca
    10441 ttttaaaagg atgatcatgc attttatctg cgtgcttctt tttgaagcga atctcgctga
    10501 tcaaaatcac tacccaagga accataccag gcaaaatact tgagctgtat acgaaaacga
    10561 aaatattttc tgactggttg atgaacattg gtagaagata atttaagatc agaccaatta
    10621 agattccacc tgagattgct acgacaggat aaaaaggcgc accgtgacga gatagtttca
    10681 gaaatttttt agacatcttt ccatttatgg caagtgtata gaccatacga cttgcgctga
    10741 aaatccctga attgcaacct gataatgccg ctgtaatcac gacaaaatta atcagtcctg
    10801 cagcaaatgt aatgccgact ttagaaaaag tttgtacaaa aggtgaacca atctcactca
    10861 attgattcca cggatagata ctcacaatga taaaaatagc accaatataa aaaatcaaga
    10921 ttcgaccgat agtggactgg acagcttcaa tgatcgtctt tttaggattc tctgcttcac
    10981 cagcagtcat tccgatcagt tcgattcctt gataggaagc aacaacgatc gataatgcaa
    11041 agaaaaatcc tttgacgcct ccagtgaaga atccaccatt tttccaaaga tttgaaatcc
    11101 ctactgcctg accatgatta ccaaatccaa ataagattac cccaagacct gcaatgatca
    11161 tcaaaatgat cgttatgact ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct
    11221 tgacagaaat aagatttgcc agcataagaa acaagacgac aatgattccg ggaataattg
    11281 ccggcaaatc aggccaccaa taattcatat aagatcctac agcaatcact tcacttattc
    11341 ctacgacaat atattgaaaa atattactcc aagcggtcaa gtagccagcc acaggatgga
    11401 tgtactcgct cgcgtaattt gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc
    11461 ctaaagcacg cataatcaaa tacaaaaaga atcctgcaat cgcataggct cccatcactg
    11521 agggccctgt ccaacgtatg gttgaggagg cacccataaa cagaccgaca ccgatcgttc
    11581 cacctagtgc gatcatttgg atatgacggg cactaagact tcgagttaac ttttcttctt
    11641 ccatacaaaa actcctttcg ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca
    11701 catgtttttc attattaggt tagccttatt gaactcttga tgtgattcta cagaaaattt
    11761 tcataaaatg cgaggaaaat tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt
    11821 ccattttagt taaactatat ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga
    11881 tttggaacgt acggccattg taattcaaga cctttcagcc gcttatcaag ggaaaactgt
    11941 tttgaaaact atcaacttga cgataaatcc acaaaaaatt accggtatta tcggaccaaa
    12001 cggtgcagga aaatcaacat ttatgaaatc tttgctagag ttgataccag ctactactgg
    12061 taaagtcacc ttttctggac agccggtaaa ctcagtccgt aaaaaaatag cctacgtgga
    12121 acagcgaagt gaattggatc tttcctttcc aattgatgta ttaggcgttg tacttttagg
    12181 aacatatcca tctttacgaa ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag
    12241 acaagctttg aaaaaagtag ggatggaaga atatgccaaa agacagatca gcgaactatc
    12301 gggtggacag cttcagagag tttttattgc aagagctcta gcccaaggag cagaatggat
    12361 ctttttagat gaaccattcg tagggattga tgcgttaagt gaacgaaaga tctttgacat
    12421 cttgcaggaa ttgaagaatt caggaaaaac gattttgatc gtccatcatg atcttcataa
    12481 agtagaagaa tatttcgatg aggttattct tttaaataaa cagctgatcg cttccggtcc
    12541 agtacaagaa gtctttacat cgaagaacct tcaattggcc tatggtgaga tcatcagaca
    12601 tttagtaaaa ggaggagaga aaaaatgatc caatccttta ttgatggatt aatgaattat
    12661 caattcctgc aatacgcttt ggtcacttcc atgcttgtag gacttgcttc tggagtcatc
    12721 ggttctttta ttattttacg agggatgtct ttgatgggag acgctatctc tcacgcagtt
    12781 cttccaggtg tagccatttc ttatatgttt ggttttagtt acatctttgg agccactgct
    12841 tttgggatgc taacagcagc agtgattggt ttcgtgactc agcggagccg attgaaaaat
    12901 gatacagcga ttggaattgt ctttagctcc ttttttgcat tagggattat cttgatttca
    12961 tatgcccaga gtgcgacaga tttgtatcat attttatttg ggaatgtctt agctgttagg
    13021 gaaagcgatt tgttgctgac agcactcgtt agtggaatcg tcttgatatt tgtctttttc
    13081 ttttataaag aattgaagat cacatcattc gatccgacaa tggcaaaagc ttatgggctg
    13141 aacacatcgc tgatccatta tcttttgatg ttctttttga cattagtggc tgtagtcagt
    13201 ttacagacag taggaacaat cttggtgatt gccatgttga tcacaccagc cgccacggct
    13261 tacttgctaa cgaaccattt actgaaaatg atcattacag ctgcaggaat cggtatgcta
    13321 agtgcagttg tcggtgtgtt tttcagttat agttacaatt ggccatcagg agctacgatc
    13381 gtgttagcat gtaccgcatt ttttatcctt gcttttttat tttctccaac aaaaggaatt
    13441 ttatttcaga aaggaagcag atagatgaaa acaaaaaaat cactcttttt aatcttggca
    13501 gttagtttct tggtattagc cggttgcgga aagcaggctt ctgaccaagc ggatgaagga
    13561 agtaaagaaa agttgtcagt cgtggctacc aattcgatct tggcggacat ggcaaaagag
    13621 gtcggaacag atcaaataga tatccacagt atcgtaccag tcggaacaga tccgcatgaa
    13681 tatgaagtac taccagaaga catcaaaaag gcaagcgatg ctgatgttat tttatacaac
    13741 ggtttgaatc ttgaaacagg taacagctgg ttcgataact tgatggaaac ggctaaaaaa
    13801 gaagaaggaa aagattattt tgcagttagc aaaaatgtag aacctcttta tttaactagc
    13861 ggtgaagaac atacaaaagc agatccccat gcttggctag acctatctaa cggaataaaa
    13921 tatgtggagg aaatcgcacg tatattctct gaaaaagatg cagaaaatgc gacactctat
    13981 aaaaaaaatg cagaagcata tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa
    14041 agttttgctt ctatcgaaga gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag
    14101 tatttttcac gagcatatga tttgccagca gcttatatat gggagatcaa tacagaaagt
    14161 caaggtacgc ctgatcaaat gaaagcgatc attgatcaga taagagcaaa agaagtaccg
    14221 gttttattcg tggaaaccag tgtagactca agaagcatgg aacgggtagc aaaagaaacg
    14281 ggtttgaaaa tctatgataa actgttcact gattccatag caaaagaagg agaacaagga
    14341 gattcttact atcagatgat gaaatggaat attgaaacga tccatgaagg tttgtcacaa
    14401 acaaaagaaa gctgaaaagt gcaaggtcta gcttgaggag cagaccttgc attttttata
    14461 agagatatga aaaaaaatta tgctgttttt cgtcgtacag ataccttttt tgaagaaatt
    14521 ccgttatagt tagagtaagg aaggaaggtg tggctatgaa ttttacacgt aaagataaat
    14581 atacgattaa agaagaagtc tttcgattga tcagccagca ggaaaatacc gacaaggatc
    14641 aagaccttca atttcttgaa gcagaattag ctaaattatt catagcaaat caaaatgaga
    14701 caacagaacc agatgaagag ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt
    14761 tagatgaaaa taccggtatc ggttattttg tgttcctttc tccagtcggc ttcacacctg
    14821 gcgtattagt taaacaaaac gacgaaataa ccgtcaaagc atggcctgca ttattttcag
    14881 tcatcaaagc agaaggagta atcaaaaaga tccagtcggc ctattataaa agtcaattta
    14941 ttgccaacag caaattaatg aatatgtttt ataatcgtct agtagaaatt gctaggcctg
    15001 ggaaaatccc gcaaacattc acgtacacac agcaaaaaaa tctctatgaa gaagtattgt
    15061 attcccgcga tctgtctgag ttttcgccat gggaagaggt aaggctccat gaactgttaa
    15121 gtacaaaacc agtttcattg aattttttta atgcattaga taacaaaaga aaattaattt
    15181 caacaaatta ttgggaagta tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg
    15241 ccgatcagca gatccagttt gtccagctga aaaaagaaaa actatattat gggttataca
    15301 gccgttaaca gcaagcgagg aagaccatga cattaaaaga agaaatcata caagaaagca
    15361 aacgattagg aattgacaaa atcgggttca ccacggcgga accttttgat tcattaaaag
    15421 aatcgttgga agaacaaaaa gcagctggcc atacttctgg ctttgaacat ccaaatatcg
    15481 atgagcgtct ttatccagaa atgacatttg atcagccgaa atcgatcatt tccattgcac
    15541 ttgcgtatcc tactaggatc catgaagaag tgcctaggga tgaaaaacgc gggcagtttg
    15601 ctcgtgcttc ttgggggatc gattaccatg atatactcag agaccgtttg aaccgattga
    15661 tcgaattcat ccaaacaaga gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc
    15721 cgcaagtaga tactggagaa ctgatcgatg tcgctgtagc acagcgagct ggtttaggat
    15781 ttattggacg aaacggattg ttgatcactg aagagttcgg ttcatttgtc tatcttggtg
    15841 agatcgttac taatattgta tttgagcctg ataaaccagg agaatttggt tgcggtgact
    15901 gtatgcgctg cgtgactgct tgtccgacaa aagcattgct tggtgacgga agaatgaatg
    15961 cccagcgatg tttgtcttat caaactcaga caaaagggat gatgccggaa gaatatcgta
    16021 aaaaaatgcg aaatgtcatt tatggatgtg atatctgcca gttagtctgc ccttacaatc
    16081 aaggaaaaga ttttcatttt catgaagaga tggaaccaaa agtagacgaa gtatatccaa
    16141 aattaaagcc aatgctttcg atgtctaata aagacttcaa acagcaattt gggcatttag
    16201 ctggttcatg gcgtgggaag aaacctttgc agagaaacgc attgattgct ttagctaatc
    16261 taggtgatcg aacggcattg cctgagattt cagaatgttt aacagatgtc cgccctgtca
    16321 tcagaggaac agcagcttgg gctattggac gtctaggaac aaaagaaccg gaaaaatggc
    16381 ttgatgtatt agatgaaata ttagctaaag aaacagaaga agtcgttata aacgaattgg
    16441 aacatgccca gaaattattg cggagaaagt taaaataaaa aaatagttaa cattattcaa
    16501 a
//
LOCUS       DAPCMT010000054        16463 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPCMT010000054 DAPCMT010000000
VERSION     DAPCMT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.054"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(27..725)
                     /locus_tag="RD743_001465"
     CDS             complement(27..725)
                     /locus_tag="RD743_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDU0563285.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="RD743_001466"
     CDS             complement(718..2070)
                     /locus_tag="RD743_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDU0563286.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="RD743_001467"
     CDS             complement(2293..3693)
                     /locus_tag="RD743_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDU0563287.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="RD743_001468"
     CDS             3854..4591
                     /locus_tag="RD743_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDU0563288.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="RD743_001469"
     CDS             4716..5195
                     /locus_tag="RD743_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDU0563289.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="RD743_001470"
     CDS             5192..5959
                     /locus_tag="RD743_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563290.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="RD743_001471"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="RD743_001471"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDU0563291.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="RD743_001472"
     CDS             complement(6862..7464)
                     /locus_tag="RD743_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDU0563292.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="RD743_001473"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="RD743_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="RD743_001474"
     CDS             8145..8729
                     /locus_tag="RD743_001474"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDU0563293.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="RD743_001475"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="RD743_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDU0563294.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="RD743_001476"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="RD743_001476"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDU0563295.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="RD743_001477"
     CDS             10630..10809
                     /locus_tag="RD743_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563296.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="RD743_001478"
     CDS             10831..11856
                     /locus_tag="RD743_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDU0563297.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="RD743_001479"
     CDS             12031..13275
                     /locus_tag="RD743_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDU0563298.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="RD743_001480"
     CDS             13428..14375
                     /locus_tag="RD743_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0563299.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="RD743_001481"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="RD743_001481"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDU0563300.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="RD743_001482"
     CDS             complement(15277..15519)
                     /locus_tag="RD743_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563301.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="RD743_001483"
     tRNA            15640..15712
                     /locus_tag="RD743_001483"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16424)
                     /locus_tag="RD743_001484"
     CDS             complement(15831..16424)
                     /locus_tag="RD743_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDU0563302.1"
                     /translation="MGTIVVVLGLLGLLFAPSIISIFSLTITGFIAFTLVFGKKDTKK
                     MFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIM
                     ILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGV
                     ARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5612 a         2941 c         3314 g         4596 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gtt
//
LOCUS       DAPCMT010000055        16440 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPCMT010000055 DAPCMT010000000
VERSION     DAPCMT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.055"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(153..551)
                     /locus_tag="RD743_001485"
     CDS             complement(153..551)
                     /locus_tag="RD743_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDU0563303.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="RD743_001486"
     CDS             complement(548..1321)
                     /locus_tag="RD743_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDU0563304.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="RD743_001487"
     CDS             complement(1318..2001)
                     /locus_tag="RD743_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDU0563305.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="RD743_001488"
     CDS             complement(2168..3055)
                     /locus_tag="RD743_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0563306.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="RD743_001489"
     CDS             complement(3052..3921)
                     /locus_tag="RD743_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0563307.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="RD743_001490"
     CDS             complement(4058..5344)
                     /locus_tag="RD743_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0563308.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="RD743_001491"
     CDS             5617..6195
                     /locus_tag="RD743_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDU0563309.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="RD743_001492"
     CDS             6195..7124
                     /locus_tag="RD743_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0563310.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="RD743_001493"
     CDS             7121..8857
                     /locus_tag="RD743_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDU0563311.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="RD743_001494"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="RD743_001494"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDU0563312.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="RD743_001495"
     CDS             complement(9753..10262)
                     /locus_tag="RD743_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDU0563313.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="RD743_001496"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="RD743_001496"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDU0563314.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="RD743_001497"
     CDS             complement(11563..12654)
                     /locus_tag="RD743_001497"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDU0563315.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="RD743_001498"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="RD743_001498"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDU0563316.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="RD743_001499"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="RD743_001499"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDU0563317.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="RD743_001500"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="RD743_001500"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDU0563318.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4752 a         3630 c         2917 g         5141 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttcctcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
//
LOCUS       DAPCMT010000056        16373 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPCMT010000056 DAPCMT010000000
VERSION     DAPCMT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.056"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="RD743_001501"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="RD743_001501"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDU0563319.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="RD743_001502"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="RD743_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDU0563320.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="RD743_001503"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="RD743_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDU0563321.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="RD743_001504"
     CDS             2857..3732
                     /locus_tag="RD743_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDU0563322.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="RD743_001505"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="RD743_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDU0563323.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="RD743_001506"
     CDS             4629..5063
                     /locus_tag="RD743_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDU0563324.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="RD743_001507"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="RD743_001507"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDU0563325.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="RD743_001508"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="RD743_001508"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDU0563326.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="RD743_001509"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="RD743_001509"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDU0563327.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="RD743_001510"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="RD743_001510"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDU0563328.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="RD743_001511"
     CDS             13334..14260
                     /locus_tag="RD743_001511"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDU0563329.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="RD743_001512"
     CDS             14442..15335
                     /locus_tag="RD743_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDU0563330.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="RD743_001513"
     CDS             15469..15594
                     /locus_tag="RD743_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0563331.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="RD743_001514"
     CDS             complement(15728..16360)
                     /locus_tag="RD743_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563332.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5815 a         2791 c         3492 g         4275 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aat
//
LOCUS       DAPCMT010000057        16289 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPCMT010000057 DAPCMT010000000
VERSION     DAPCMT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.057"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            69..1496
                     /locus_tag="RD743_001515"
     CDS             69..1496
                     /locus_tag="RD743_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0563333.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1588..1953)
                     /locus_tag="RD743_001516"
     CDS             complement(1588..1953)
                     /locus_tag="RD743_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDU0563334.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1950..2315)
                     /locus_tag="RD743_001517"
     CDS             complement(1950..2315)
                     /locus_tag="RD743_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDU0563335.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2507..2755)
                     /locus_tag="RD743_001518"
     CDS             complement(2507..2755)
                     /locus_tag="RD743_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563336.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2754..2882
                     /locus_tag="RD743_001519"
     CDS             2754..2882
                     /locus_tag="RD743_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDU0563337.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2939..4633
                     /locus_tag="RD743_001520"
     CDS             2939..4633
                     /locus_tag="RD743_001520"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDU0563338.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4637..4756
                     /locus_tag="RD743_001521"
                     /pseudo
     CDS             4637..4756
                     /locus_tag="RD743_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4733..4906
                     /locus_tag="RD743_001522"
     CDS             4733..4906
                     /locus_tag="RD743_001522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563339.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5284..6417
                     /locus_tag="RD743_001523"
     CDS             5284..6417
                     /locus_tag="RD743_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDU0563340.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6448..6768
                     /locus_tag="RD743_001524"
     CDS             6448..6768
                     /locus_tag="RD743_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDU0563341.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6797..7261)
                     /locus_tag="RD743_001525"
     CDS             complement(6797..7261)
                     /locus_tag="RD743_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0563342.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7393..8646)
                     /locus_tag="RD743_001526"
     CDS             complement(7393..8646)
                     /locus_tag="RD743_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0563343.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8675..8992)
                     /locus_tag="RD743_001527"
     CDS             complement(8675..8992)
                     /locus_tag="RD743_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDU0563344.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9018..9338)
                     /locus_tag="RD743_001528"
     CDS             complement(9018..9338)
                     /locus_tag="RD743_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0563345.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9802..11217)
                     /locus_tag="RD743_001529"
     CDS             complement(9802..11217)
                     /locus_tag="RD743_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0563346.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11234..12550)
                     /locus_tag="RD743_001530"
     CDS             complement(11234..12550)
                     /locus_tag="RD743_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0563347.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12700..13398)
                     /locus_tag="RD743_001531"
     CDS             complement(12700..13398)
                     /locus_tag="RD743_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDU0563348.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13687..14643)
                     /locus_tag="RD743_001532"
     CDS             complement(13687..14643)
                     /locus_tag="RD743_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDU0563349.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14756..15589)
                     /gene="msrA"
                     /locus_tag="RD743_001533"
     CDS             complement(14756..15589)
                     /gene="msrA"
                     /locus_tag="RD743_001533"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDU0563350.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15764..>16289)
                     /locus_tag="RD743_001534"
     CDS             complement(15764..>16289)
                     /locus_tag="RD743_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563351.1"
                     /translation="FYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTT
                     KGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVIT
                     LPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLT
                     AYLCLFFKIVFHKR"
BASE COUNT         5081 a         3278 c         2764 g         5166 t
ORIGIN      
        1 tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag
       61 gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg
      121 ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt
      181 gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg
      241 cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat
      301 gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt
      361 tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca
      421 aagcttattc attaaaaaaa caagtattag cttactttaa tgactgcggg atcgaaatcg
      481 accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt
      541 atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc
      601 gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa
      661 tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag
      721 attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat
      781 gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac
      841 tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac
      901 agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg
      961 aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc
     1021 tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa
     1081 atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc
     1141 agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga
     1201 gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg
     1261 ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg
     1321 caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc
     1381 gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc
     1441 ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg
     1501 ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat
     1561 ctcttgatac acgtcaggct cacaacctca cttattttgg acatctaggg gtccgatctc
     1621 tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat
     1681 atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa
     1741 acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc ttactcggtc
     1801 gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac
     1861 accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata
     1921 tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct
     1981 ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt
     2041 gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg
     2101 actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta
     2161 cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga
     2221 acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc
     2281 cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt
     2341 tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct
     2401 aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca
     2461 taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca
     2521 gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac
     2581 catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag
     2641 acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta
     2701 ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta
     2761 aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag
     2821 gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct
     2881 agcatcctat ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat
     2941 gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta
     3001 tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca
     3061 cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca
     3121 tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac
     3181 ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac
     3241 tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt
     3301 gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg
     3361 tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat
     3421 cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat
     3481 gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat
     3541 gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg
     3601 ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg
     3661 caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga
     3721 tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt
     3781 tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag attcagctac
     3841 tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct
     3901 ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga
     3961 tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa
     4021 tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac
     4081 cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca
     4141 accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc
     4201 aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct
     4261 agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa
     4321 agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc
     4381 cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac
     4441 caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca
     4501 aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg
     4561 tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa
     4621 aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac
     4681 caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc
     4741 ttttgaacac cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag taataagagc
     4801 agccaagcta aaacagcttc ttatgttttt gacttggtta gacttattac cgcagatgca
     4861 agcttttgaa caccgtttac caagaggtta tgaaaaaggc gtttagtttc gagtatgaag
     4921 aaaaattttg tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa taccgagaaa
     4981 ctgatccctt ctgtcataac ctctttttat ttatgtctgt ttctttttga aaacaactat
     5041 tttttcaacg tttactctct tgccaatcca acatcatcta acggatacaa ctatttcaat
     5101 gattaccatt acactaaaaa atcggaaaca cacctctccg aacacaaata cataagacta
     5161 tagaatgttt catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa
     5221 aatgaaatta tctgtagtac taataatgca gacaatttta tgaatctgaa aggagcatct
     5281 ccaatgaaag cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat
     5341 ccaaaaatat tagaaccaac agatgcaatc atccgtatca cagcaacagc catttgcggc
     5401 tccgatctgc atctttacca tcatggtgaa gcagttttgg aaaaagggta tgttgtagga
     5461 cacgaaccaa tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg
     5521 caacgtgtgg ttatcccttt taatattagt tgtggtcatt gtcatttctg cctgaatcac
     5581 atggaaagcc aatgtgatga atcaaatcca gatcagttat atggcgggct tttcggattt
     5641 ggcaagttga acggtaatca ttggggtggt caagcggaat atctaagagt tccttatgca
     5701 gactctactt catttgttgt accagataat gatcttcctg atgaaaaagt tttatttcta
     5761 tcagatattt tgcccacagc ttattggagc gttgaaaacg caggagtaaa aaaaggcgat
     5821 acagttgtga ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg
     5881 gctggagcag agcgcgtaat cgcagtagat ccagtacagc accgtctaga taaagcggct
     5941 aattacaatg gggttgaaac ttatttctta gaagacacgg cacaagctgg aacagaattg
     6001 tatgaactgg ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat ggacggatta
     6061 gagcctgtca aagaaaaagc gaaaaattta gtaagcttac aaagcggcac aatctctcca
     6121 ttacagatgg catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat
     6181 atgactccag cttcttctta cccgctgcaa gaatttttta tgcgggatat cgctgtcaag
     6241 catggacagg caccaattat ccatctaatg ccaaaaattt acgcaatgat tgcagaagga
     6301 ctattcgatc ctacacaaat cattactcat tcgctgccac tatcagaagc agcacaagca
     6361 tatgagatct ttgacaaaaa agaagataaa aatataaaag ttgttttgaa accttgaatt
     6421 cagtaaaaaa tacaggtgat tttctttatg cccaaaatca aacgaaatac ccatgagatc
     6481 gaaataggaa aagaagaaga tatcgaaata cgaggacgtc gcttccgctt ctatttccaa
     6541 aatcgttaca cattgatctc actggctgtc gatttattaa ccgggatttt ctatattttt
     6601 ggaagccttg cttccttaac taaaatcccc gatcgatatg gcacctattt ttatttggct
     6661 ggtgctattt tcttgacgat tcgtcctatt ctcagaatcg taaggaacat ttttatttac
     6721 gacgaaaaaa aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa
     6781 tctcatgcct cttttttcac cctatcgtat cactttgata cagacgagcg gtcgtcccac
     6841 tttcttcctt gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat
     6901 ctgtaattaa gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg atttccgcta
     6961 atatccgctt ggctatcccc tgcttacgct tttccggttc aacaaacagg gcacaaagat
     7021 ttggtgtcag atcagggcgg ttatggaagt cgttttcaat gacgcccgct cctccgacga
     7081 tggtttcttt ttcatctaag accacatacc attgaggaat actttcctta ttagcgattc
     7141 cagtacgaat actttcctca tacacaacag caggaactcc ccattttgat gaaaaccaat
     7201 tcgcgctttg ttttactaat tctgggtgtt ctcttaattt gatccagtta tatgtaacca
     7261 atgtttaaac ctccgactaa aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa
     7321 ttagcaatga caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg
     7381 ttttttgatt aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt
     7441 gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc
     7501 tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat
     7561 aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac
     7621 cattggtgtc aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa
     7681 catcgttggt tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat
     7741 atatggtttg tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc
     7801 tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct
     7861 atgtccagct gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat
     7921 caaaggatcg accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa
     7981 tgcaagcagt aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac
     8041 tgtatagaaa gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa
     8101 agcaaacgca gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc
     8161 cggtggcaca ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa
     8221 gatttctgtt gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc
     8281 taatgctccg cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc
     8341 ttttcggtag ccgttagcta aatgataagc aagcaagaaa gcaatcaaga cagaaagaat
     8401 ggcaaatgta ccgttccaag cagcagcccc gaatcctttc catgattcac caccaaaaat
     8461 tgagttcatc aattcttgga acccgggaat cggtaagtta ttgatcatta ctgctaatgc
     8521 tcctaagatc attaacggca tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg
     8581 ttgtgcacca atttttgatg ctacaggtat aaaatgtttc tccataaatg caacaaattt
     8641 atccataaat cttccctctt ttctttactg tagattacgc gttcaatcga cgatacagat
     8701 taatgatctc ttttgctaaa tctgtaaaag ctatagatgt catcagatga tcttgactgt
     8761 ggaccgtcaa aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg
     8821 tttgagagtg atgtgcttgg actaaagatt cctctgcatc tttgattttt tcatctgcta
     8881 aagtgaaatc accttttttc gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc
     8941 cgccaaacat gatcagaccc atgatggctt ctagattttt ttgatcttcc atttattgta
     9001 tcctccgata atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca
     9061 ccattcatca tgccatagtc agccatattg attacatcta aaggaatgcc tttaggtgct
     9121 agcttttcag caaattgatt ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct
     9181 acatttttag attctagttg attatccgca tctgaagcag atacagcaaa gatgtctgct
     9241 tccattcctt ttgcttctgc tgccttttgc atttttgtga ctaggagact tgtactcatt
     9301 ccagctgaac atacaagcat gattgttttc ttttccattt taaacacgtt cctttctata
     9361 cttacacttg tatataagca agtagcgtgc caactttaaa agcgctttaa aaagttgata
     9421 tcatcacatt taaggtctat accaagttgc atacacacta aaaaagaaag cgcacactaa
     9481 acacacaaat taatttttat tacctttttt cttttaacgt gcacaaaaaa agttgtgaca
     9541 caaaacttgt cacaactttt ttcaaataaa cggtattcaa aagcttgcat ctgcggtaat
     9601 aagtccaacc aagttaaaaa cataagatgt tgtttttact ttgctattct tattacttga
     9661 tgcaggacag ctttttcaca acctctcaat aaacggtgtt caatcagctg accctgggta
     9721 ttagctcaaa acgctggatt cacaacctct tttatttcaa ataaccagta aatacaatga
     9781 acatctacaa aaaacttcta tttatttata gatttcgtca ggcaaatcca attctctctt
     9841 atcaatcaaa gtctgatacc acgaagcaga ttttttcaat gttctgcttc gttcatcgtt
     9901 caattcgatt cgaactaatc catatcgatt cttaaatgca ttcattggtg acacacagtc
     9961 agtgaatgcc cataacatat agccttcgca atttgcgcct tcttctgttg ctttgatcaa
    10021 gtaatgcaaa tgctgtccga taaattcgat acggtaatca tcttgcacga ttcctttctc
    10081 atccatatac tgttcttcat attctcttcc cataccgttt tctgttatca gccaagggat
    10141 attatgataa ttttctttta gatacatggc tatatcatac atgattttcg gatagatttc
    10201 ccatcccctg gaattattca ttttcttccc aggtaaatca aagacttcat aatatcgttc
    10261 aggatggaaa ggtgtctctt cattccattg ataacgaggg gcgcgaacgc gtttaggata
    10321 atattgattg attccaagga aatctactgt atgatgctga atcgtttcta aatcctctgt
    10381 tgcaggatcg aaataaatcg attgtttttg acacaactcc aggaactctt ctgtgtagta
    10441 tccttttacc atcggatcaa aaaatactcg attataaaac aaatcataca tataagcagc
    10501 tttttggtct tctggagaat tggatcgagc atagaccatt tcaggattta aaatacagcc
    10561 aatccttcca ccataatttc cgctgcggta aatctctact gctttagccg ttgctaaaac
    10621 tttatgataa ttccacatca tccacttttt tgtgttttgt tcatgcggcc agcgaacagc
    10681 atccaaatag caacgcgttt ggggaacgat tggttcgttg aatgtaaacc agttttttac
    10741 cctatcaccg taacgatcaa aggcaatttg agcatactta gcataaaaat caactacttt
    10801 tcttgaagac cagccatcat atttttccaa aagaaaagct gggacttcat aatgttccaa
    10861 acaaatcatc ggttctacgt ctgcttcgat caaagagtcg atcatttcac ttatatgctg
    10921 agcatattct tcatctacta tacagttctc ataatcagta aaaaaacgag cccaattgat
    10981 agaagtccga taatgtgtca gatggatgga tttcatcaga tccacatcct gctggtattg
    11041 ttccataaaa ttagttgcta cggctgggcc atacccatta tgccaaacaa aatgttcatt
    11101 tttataccag ctatctataa acgaatcctg tccttctttt tttcctttcc aaccttccgt
    11161 ttgccaagca gaagatgcag caccaagaat aaaatttttt ggaatagtaa ttttcatgat
    11221 ttcctcctgt tttttagatc ccatcatttt ccacacgatt tgctactttt acaaatggca
    11281 gatagatcaa aatagaaaca ataatacaaa caagttgcgt aataactgca ccgatatttc
    11341 ctgctgttgc cagataagca ttgacgatcg gcggcattgg ccaagggacc attacaactg
    11401 ctttcgccgc aaatcctaaa gcggttgcaa aataaccaat cgttccagtg ataagaggcg
    11461 ttaagataaa cggaatagct aaaattggat tcagcatgat cggcatacca aaaataactg
    11521 gttcattgat gttgaagatc ccaggagcaa gggataactt ggtgatttgt ttcatatctt
    11581 cccgttttcc aactaacaat actgcaatca gtaaaccgat cgtgactcca gacccgccca
    11641 tactcatata catatcccaa aatggcatag taataatgtt cggtacttct tttccagctt
    11701 caaaagcttc cgtatttaca gcaattgccg ctaaaagcaa cggttcccgt atcggcttca
    11761 ccatttgatt tccatggata ccaatcaccc aaaagacttg cgcaataaac atcaatagca
    11821 aaatacccgg taacccttgt acgacacctt ctaatgggcg ttgtacgaca ttgtaaatga
    11881 tatcataaag gtacattcct gtaagttctt tgatgataaa tcccgcagcc gcgatacccg
    11941 atacagtcag aatcgtcggg aataaagcag aaaaactagc agaaacattc gttggcacgg
    12001 tatcaggcat tctcagctgt agtttcttct gtttcctcaa ccagcaataa agttcaatag
    12061 aaaggatgga gaccaacatt cccaaaaata accctttcgt atctgtatat tgtttagcca
    12121 atacgttttc tactataaca gtctgatttt cattcactaa ttcataaaca gttggattaa
    12181 cagataaata agacatcact gcgatgatac cagggaatac accatccact ttattcagct
    12241 tggctaattc gatccctatc agaaaaacag catagatggt caagaaactt agtgtgacat
    12301 agctgatggc agatgcgata ggctttaatt cttctaaaaa acgcaagaaa cttatctgtg
    12361 ctaagccatt ttccgagtca aagaccattg ctgaaaacaa tgtgccaaaa gcacccgtaa
    12421 taattaccgg aatcaatgca gcaaatgcgt tttttatcac catgatatac cttaaattat
    12481 tgattctcgt tgccacgttc cctaaaacat tggctaattt atccataaaa ctcattccag
    12541 atcccctcat tcctgcttct tctccttgac gtcatctctt tttgtctatt agtcagcgct
    12601 tacatttcga tgaacagaaa ctatttactc ctctttttta ccaaatataa aacaaaaatt
    12661 catctgatgt atttatcata aaatacatca gatgaactgt caattagttt ctttttctcc
    12721 tattattttc tgtaattctc tccgatttat tgccatatgg acagtcattg catcaaacgc
    12781 agcaagtgca ttccgccttt cgatggcctg aacgatctct gtatgaatat cgatcgtctc
    12841 gattttcaga tgctggatat cataattttt attaaacaaa gcaattgatt cattaatgat
    12901 tgggataata tgcagcaagg ctacgtttcc gctagcttct gcaatcatcg tatgaaaatc
    12961 gatgtctaat tgaacatgtt cttgtgtgcc ttctcgaaaa gaagtttcaa tcttttgcgt
    13021 taattttttc atttggacga tttgattctt cgttgcatat tccgctgcca aacctgccat
    13081 cttaggctcc aataaatatc tcagttcaaa taagtctcgt atcatccgat cctgatcttc
    13141 aatcaaagaa aaaccgagtg ggtcattggc aatccctcgc ttatcactga caaatgtccc
    13201 cgctccttgc cgaacttcaa ggatattcct ggaagctaga gcccgcacag cttctcgcac
    13261 cgtgcttcgc cccacctcta aataatcgga caattcatat tcattcggca gtttatctcc
    13321 tacttgcaac tgcatttcct ttaaatatct aaggatcttg tcagtagtta cttctactag
    13381 ggttttattt tcttccaaac cacatacctc cttttcatct gatgtatttt ttcgtaatta
    13441 ttataacacg tttgaatcat ctgatgattt tttgtataca aaattgatta atccgttcat
    13501 agacatacaa acgaagaaga tatttaccat aaaaaagact aggataaaat tcactcctag
    13561 ccttcgatca tttttatttt gaacgacaaa aaatatgatg ttcaataaca gacgatcttc
    13621 attcgattct tgatgtcatc ttttgttttt tttaatatat ggataaaaag tttgattaca
    13681 tcgtttctat tctagaattt ggtcgattct caattcttct tctggtgaga aatctaaatt
    13741 atccatcatt cgaacacttt cttgtaactg acttaaccga cttgctccga ccagtacact
    13801 tgtaacagat ttttgtcgca aattccatgc aagggccatt tgcgctaatg attgtcctcg
    13861 tgatacggcg attgtttgca gtgctttgat tttttccaga gttgatccaa cttgttcttc
    13921 tgaaaggaat ggtatttcct tacgatgtgc tctagaatct tcaggaatac catgtagata
    13981 acgatttgtc agcaaccctt gagcaagcgg actgaacgtg attgccccta gcccttcttc
    14041 ttctaatact ggagatagat cgtcttctag tagtgcacga gaaaacatat tgtagcgcat
    14101 ctgatgaata atgaacggtg cttcctttct ttttaaaatt tcagtcattt tttttgtatc
    14161 ttctccatta tagttggata taccaatata taatgctttt ccttgtctta caagttgcat
    14221 caaagcttct gctgtttctt ccaaaggtgt atcagggtct gggcgatgat gatagaagat
    14281 gtcaacataa tctagcccca ttctttggag actttggtca caacttgaaa tgatggattt
    14341 acgagagccc cattccccat atggcccttc ccacatatga taacctgctt tgctagaaat
    14401 aatcagttca tcacggtagg cttgaaaatc ttctctaaaa atacgcccaa agtttttctc
    14461 tgcagctcca gcagggcctc cataattatt cgcaaggtcg aaatgggtaa ttcccatgtc
    14521 gaatgcgcct cttaaaatct ctctttgatt atggatagga tcataatcac caaaattttg
    14581 ccataatccg agagataaaa tcggtaattt caaacctgaa tgaccggatt tacgatattc
    14641 cattttttca taacgtgcac tatctgcaat atacaatttt tctcctcctc atcaaatctt
    14701 tttaaaacgc tttctttttt ctatttttag tttactacat tttaaagaaa aggagttaat
    14761 caaaatgatt ttttcgtata tttttttgcc atttgttttt tactcgttga tagatcagaa
    14821 attgattacg tgcttgctgg atttttttat aacgtttcgg ttgttttttg taaaattgct
    14881 gatgatagtt ttctgctggc caaaacgtac cggctggttc gatttttgtg acgattggtt
    14941 ggtggaaagt tcctgaactg tctagtttct gttttgattg ttccgctaat tctttttgtc
    15001 gttcgacttg ataaaaaatc actggacgat attgcttccc tctatcttga aattgtccaa
    15061 atgtatctgt gggatcagta atttgccaat agattgtcag caactcgcta taacttattt
    15121 ctcttgtgtc ataaatgatc tccacagctt ccacatgacc tgtgtaacca ccacttactt
    15181 gatcataatt tggtttttca acatgtccgc ccgtgtatcc tgacaaaaca gataggatcc
    15241 cttttttcga ttcaaacggc tcgaccatac accagaaaca tccccctgca aatactgcac
    15301 gttcttgata gtcagcatct tgcatggcat gcttggaaaa gtcatatttt tgctccttat
    15361 caaatccttc cgtaatcaat aaataaaagt ccattacatc aggcgtcaga ttcattcgga
    15421 tagctaatgg acgtagtgtt gcttctagtt ttgataactg ttctttaacg gatatatttt
    15481 ctcttatgtc attctttgtc tgaatcaata cttttcgttc ccaatctcgt gtattcggat
    15541 tcaaaatcag attataaagt gtttgaatga atatttgttc ctcatccata gattctcctc
    15601 ctttccgaag aagacaagct agtgacttta gctgtaattt atcataaagt gaggaaataa
    15661 gcgattactt catcccccta cagatttttc gtataaaaaa gagagaatcc ttatcgacag
    15721 attctccttt ttaatcaatt tattttataa atgaaaagtt aatttacctt ttatggaaaa
    15781 ctattttaaa aaataaacat aaatatgcag ttaatgtaac aagataagaa ataatagtcg
    15841 ttactgtata acttgtgctt actttaatat cagcaactcc tttgactccg cttatttgta
    15901 ccaatccatt agcattcacc tgctccttta caggttgatg atttatttct acattcgttc
    15961 ctaaataagc atatacagga agtgtgatta cttggctctt ctcattagtt actctatata
    16021 tgatagaact accagttgtt ttataaggaa ggtttaccca ttgactgcca atctttgctt
    16081 cgtgttttat tatatgctcc ttattctcca atgaagcgat aggagaataa tcatataaaa
    16141 aataatccat tcctttagtg gtatacgcct ctactgtttc tttgtttaca acaatatgtc
    16201 ccggctgaga aattattttt tgattgaaat tttttttact agaaacagtt aaagtgatta
    16261 ataacaaaac agtcataaat aaataaaat
//
LOCUS       DAPCMT010000058        15683 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPCMT010000058 DAPCMT010000000
VERSION     DAPCMT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.058"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(243..609)
                     /gene="ssrA"
                     /locus_tag="RD743_001535"
     tmRNA           complement(243..609)
                     /gene="ssrA"
                     /locus_tag="RD743_001535"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(780..1553)
                     /locus_tag="RD743_001536"
     CDS             complement(780..1553)
                     /locus_tag="RD743_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0563352.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1697..2047
                     /locus_tag="RD743_001537"
     CDS             1697..2047
                     /locus_tag="RD743_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDU0563353.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2028..2744
                     /locus_tag="RD743_001538"
     CDS             2028..2744
                     /locus_tag="RD743_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0563354.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2744..4192
                     /locus_tag="RD743_001539"
     CDS             2744..4192
                     /locus_tag="RD743_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0563355.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4262..4771)
                     /locus_tag="RD743_001540"
     CDS             complement(4262..4771)
                     /locus_tag="RD743_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563356.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4761..5486)
                     /locus_tag="RD743_001541"
     CDS             complement(4761..5486)
                     /locus_tag="RD743_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563357.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5709..7829)
                     /locus_tag="RD743_001542"
     CDS             complement(5709..7829)
                     /locus_tag="RD743_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDU0563358.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8059..9237
                     /locus_tag="RD743_001543"
     CDS             8059..9237
                     /locus_tag="RD743_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDU0563359.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGMLIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9253..10011
                     /gene="aroD"
                     /locus_tag="RD743_001544"
     CDS             9253..10011
                     /gene="aroD"
                     /locus_tag="RD743_001544"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDU0563360.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10034..10519
                     /gene="ybaK"
                     /locus_tag="RD743_001545"
     CDS             10034..10519
                     /gene="ybaK"
                     /locus_tag="RD743_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDU0563361.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10590..11843)
                     /locus_tag="RD743_001546"
     CDS             complement(10590..11843)
                     /locus_tag="RD743_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDU0563362.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11961..13310
                     /gene="gdhA"
                     /locus_tag="RD743_001547"
     CDS             11961..13310
                     /gene="gdhA"
                     /locus_tag="RD743_001547"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDU0563363.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13422..14768
                     /locus_tag="RD743_001548"
     CDS             13422..14768
                     /locus_tag="RD743_001548"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDU0563364.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15076..15462)
                     /locus_tag="RD743_001549"
     CDS             complement(15076..15462)
                     /locus_tag="RD743_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDU0563365.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15511..>15683)
                     /locus_tag="RD743_001550"
     CDS             complement(15511..>15683)
                     /locus_tag="RD743_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDU0563366.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         5120 a         2809 c         3158 g         4596 t
ORIGIN      
        1 ttccttccat cagcataaaa gttcacctca aaaagagtgt acgaacaaaa gttctttttg
       61 taaaccttaa aatagatttt cctgaatacg tattggggat ttctaaatgt ttgaatgttt
      121 ttttgaatgc aaaatcaatg caaaaaattt tgtatttctc tacttttctc tccttgacca
      181 aaataaaaaa acgttgattt aacaacgttt ttcatacatg tttatctgtc tcaaccatac
      241 atatatggag acggcgggag tcgaacccgc gtccaaacac atcgccactt aaaaatctac
      301 gttcatagtc aactcattta aggattcact ttacagcttg ccgagtgaca ggcattctgt
      361 atcgctagtc tgatgttctc tttttcaact tacagacgga aagttgaaac gtatcccact
      421 aaagttagga cccttactcg agcacatggg cgatgccgag aggatctacg ctaactgttt
      481 ttaggcagct aaagcgtaag agttgttttc gtttttagca gttatattta actgtaacgt
      541 ttttacgtag acgtaaccta cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt
      601 aacgtccccg ttagctaaca agggttacgt gtatttttca tacaaaatta gtatagcata
      661 tattgggata aattcaataa aaaagcgaat catatcccat tatttatata ataactgccc
      721 ctttcttaag aaaaaataag cgattttctt aagaaaagag gtagcttcaa aaaaagatct
      781 taaatcagat caaaatactt caatgcgtgg acaattccgc cttctgtatt tttctttgtg
      841 ataaaggtag aaagttcttt taatccgtca taggcgttgc ccatagcgat tttgttgtcg
      901 caagcttcca acaaggcaaa atcattcgga ccatcgccaa atccaaaagt aggaactttt
      961 tccagctgaa gttctttttg cagacgtttg acacctgccc cttttgagat tcctttcttc
     1021 actgtatcga tcgagtaagg accattccga taaaaagtta gctcaggaaa atgttcgtga
     1081 tagtattcgt cacctgattg agataagatc agcaacatat ttactgtgtt ttctttaaag
     1141 cctaaaggat caatttccgg aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt
     1201 tggctatgac cggtacacca gattttctga tcgttatagt aagaaagttc atgaccgttc
     1261 aactgtacat gatcatacat tttttgacat tcttctgaag aaaatgtttg agaatagatt
     1321 tcttcgccgt ctactcgaat gaaagaacca ttcatggtga ccgcagaagt aatgcccgca
     1381 tcttccatga tagagtggat ttctgcttct gtacgtcctg tagcgatgac ggggagaaca
     1441 ttgttttctt ttaatgcgtt catagctttt gccacttcgg gtgtgatttt tgatttcgca
     1501 tctagtaatg ttccatcaag atcaaagaaa gtgattgcac gataatttga cacaaataaa
     1561 actccttttt tattggaata agtttatctt aacaaagaat gaacaaaaga caaacctttc
     1621 gtgaagaatt cctgaacata ttggttcagt caacaattta gagtatacta taagaagaga
     1681 atttaggagg gaaagaatga tttttttcag tattggtatc ttattcttaa taattggcag
     1741 tctttttcgg attctgccct caaaaggaaa tctgcctttt tatgggtatc attcgcctct
     1801 tgccgcaaaa acagacgctc attggcggct ggctcaaaag acaagcggaa actggttttt
     1861 tcttatgggc ttactgatgg cattgatcgg ctattattta aagacatctg ggcatacgaa
     1921 ttattttctg attgaaatgc ttctgctagt atttccaatt atgcctattt ttattatgac
     1981 agaaaagaaa ttacagaaat atgacctgga aactggaggg aacgataatg aatattttaa
     2041 tgattgaaga taatcaatct gtttctgaaa tgatgcagat gtttttctta aatgaaggtt
     2101 gggaagcaac tttcaagtat gatggaaaag aagggctcga tgccttttta gaaaacccac
     2161 aacattggga tatgattaca ctagacctga atttgcccac actggacggt atgctagtag
     2221 gaagagagat ccgcaaagtt tcaagtactg tgccgattat catgctgaca gctagagatt
     2281 ctgaaagcga tcaggtgatc ggcttagaga tgggggcaga tgattatgtc acaaaacctt
     2341 tcagtcctct gacgcttatt gctcgaatga aagctttgca tcgccgtagt gagttggcag
     2401 aagcaagaga agaagcagca gtttcagatg atcattttga tatcgagact agtcatttca
     2461 agatgaatac gaaaacacga gaagcgtatc tggacaaccg gccaattgaa ggactgacgc
     2521 ctaaagagtt cgatctattg tttactttag caaaaaaacc acgtcaagta ttctctcggg
     2581 agcagttgct tgaattagtc tgggattatc agtactttgg tgatgaacgg acagtggacg
     2641 cccatatcaa gaaattacga caaaaaatcg aaaaggtcgg accacaagtc atccaaactg
     2701 tttggggagt cggctacaaa ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca
     2761 gcaactctta gctttttgcg gaatggtcgc gttgatctta ttgatcgttg gagtttcttt
     2821 tacacaactg acaaaacaaa cgattgaaga aaacaattac cagcaactgt ttggatatgc
     2881 tgaatcagtt gaaaagacaa cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga
     2941 tcaggcgttt caaaatgcat tgctgctgac agagcaagta ctgacagaac aaaatgtgaa
     3001 ctttatattt atcgataaaa acgaacgtgt catttatccg actaatacag ctttactgaa
     3061 ttttacctta acagctgatc aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac
     3121 atcgaataaa aatattttag gacaagcaca ggcgacctct tacgcacttg tgccatttaa
     3181 tttgaatcat gaattctatg gagcacttgt agttagtcaa cctgcgcgaa atatcgataa
     3241 cagcgttcgt ccaattacac tgaacctgtt caaaggattt attttttcca gtatcgttgc
     3301 agttattgcc agctatgctt ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa
     3361 tgcgacgaaa gaagtgacga atggtaattt tgatgtacag ctccccgtac atgacaaaga
     3421 tgaatttgat gagttagcag atgatttcaa taaaatgacg aattcattga aagaatctca
     3481 agcagaaatt gaagaacaag aaaatcgtcg aagacaattt atggcagatg cttctcacga
     3541 gatgagaaca cctcttacga caatcaacgg acttctggaa gggttagaat acaatgcaat
     3601 ccctgaaaat cagcgagaaa acgcgattaa attgatgaaa aatgaaacag agcgtctgat
     3661 ccgtttggtg aatgaaaact tggattatga aaaaatccgt acgaatcaaa tcagtatggt
     3721 cattaaaaaa ttcaatggaa cagaaacatt gaaaaatatt gttgcacagt tagaagctaa
     3781 agcagaagca gcaggagata cattgacatt gaaagccgtt gatgatattg atgtttacgc
     3841 tgattatgac cgatttgtcc aaatcatggt caatattatt caaaacgcga tacagtttac
     3901 tgaaaacggc caaatcacga ttactttaga aaaaggctat cttgaaacga tcatcacgat
     3961 tgaagatacg ggaattggaa tgtctgaaca acaaatgaaa agtatctggg atcgttatta
     4021 taaagtagat ccttcaagaa aaaatacaaa atatggtgaa tcaggattag gtttaccaat
     4081 cgtgcagcaa cttgtacgct tgcataaagg aaaattagaa gtagaaagcg aactaggaaa
     4141 aggaacaaaa ttcgctgttc gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag
     4201 cggtataaaa gatcatgaat cgatcttcta taccgcttgt ttttactata aaatgattga
     4261 tttagcccgc gggctctaaa cgatcaatat aaggactttg agcaatagta gcatcatgag
     4321 taaagagtaa gataccttga ttttcttcat gagctaaata agaaagcaag tggagtagct
     4381 cttggatctc caatacacta agttcatcaa atgtatcttc aataacaata ttgtttcgct
     4441 tggaaagcaa ggcatgaagt aattgcagct ttactttttc aaaagacgtc aactcagctg
     4501 ccgcacaatt aagcaaggaa ggagagatat caagtttttc ttgataggcc aaacgatctt
     4561 gtttgatttc tttttccggt acatccaaca ggacatggtc tttaagattc agataaggaa
     4621 acatcggcca atttttttgg atgatcgcaa aattatccag ttttgcaaca agaacctctt
     4681 ttttcaagtg ctccaatatt ttatgctgtt tttcgagagg acacaaaata acggtcaagt
     4741 gttcagcatg aatcaaatat tcaggtttca ttacgtccag gcctcctact ttgcaaatga
     4801 tggataaaaa aagtgaaagg gaatagacta agaatgagac agcatccact gagcccaata
     4861 agagtttggg caaatccctt aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg
     4921 gtaaaaaaag tctgttgatc catcggaatt gtaagtccgc ttaccgaagt caagatatca
     4981 cggatatctg gcagttcagt agtctttaag acaagaaaat tgatcctttg aagaagcgct
     5041 tcataaactg actggaagct tacgatccac aataaaacca agatacaaca aagaaagaga
     5101 ggaaagaaca gctgccagaa aataaaaagg aataatttgg ttttagaaag accagtcagg
     5161 cgccaattga tcaattcctg cttgttttgc ctgactgaat agtaaaagaa aaagataaaa
     5221 aggaaggcaa atagaaaaaa taaactgaat atgcctttta tataaaatcg gctagctaat
     5281 tctaaagccg gaagaagctc tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg
     5341 acttgattgg ctaaagccga ttgcaaatgg taaagagtca ggcatgctat caataaaaaa
     5401 gcagaaaagc ttgaaaataa acaatagagt attgtctgct ttttgtaata aattaatcga
     5461 gcagtagcat agcggattat tttcatactt ttcacctcta taaaaagtat agaaaatgaa
     5521 tatgaataaa gtatgaaatt cctttagtga aatcacgaat taaaaccaga aaatattttg
     5581 taaaaagcaa gaggttgtga agaagctgcc ctattctatt accgtagatg caagcttttg
     5641 aacactgttt attcgaaaag aggagcagcg acaataattt tgtcgctgct ccttttaaat
     5701 ttattttgtc attcggaaga acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt
     5761 ttataaagat caacagttga ttgtccgttg tgttcactaa agacactagt tgacttgtca
     5821 ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc ataatcactg
     5881 gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc attattgatt
     5941 tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat ttcattctgt
     6001 acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat cgtaccaccg
     6061 tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag ctgttcatga
     6121 tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac acctagtaag
     6181 tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat tccgccctgt
     6241 gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt gtcgtagttt
     6301 gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct tgaattcgag
     6361 acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc gctttctttc
     6421 aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc aaagtaaccg
     6481 ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt ggtgaatttt
     6541 gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg ttgcagacgt
     6601 tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa
     6661 ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc taaattcttg
     6721 tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc actagtgtaa
     6781 ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc gcctaattgt
     6841 gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt ttccgcatcg
     6901 cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg
     6961 tggtaaagac tattgatgaa aggtgtaact tcatgctgga gaccattctc atctgtcaat
     7021 ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac attttttcct
     7081 ttagcgagcc cgaacaaatc gtcatttggt gctgcataat gttcttttac ataattttct
     7141 acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg gtaagtttga
     7201 acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata atcacgagag
     7261 aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt tcctgagaaa
     7321 atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc
     7381 aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat ccaatagatc
     7441 acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc tactttccca
     7501 gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata agtgacattc
     7561 gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata agcaactcta
     7621 gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat agggttgata
     7681 aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac taaataggca
     7741 aaaatcgttt tcaaccaaag taaaacgact agtaaagaaa aaaatccgag cctcttattc
     7801 aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact tcttaatcac
     7861 agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt actagtatga
     7921 caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt tctttaagct
     7981 tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg ggaagagaaa
     8041 gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa
     8101 aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa
     8161 tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag
     8221 aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca atcttttttt
     8281 gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga tgattctact
     8341 accgcttttc gtatattcaa tggagaagga gacggaatcg gcggtctgat tgttgattat
     8401 tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta
     8461 ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga gaaaattcga
     8521 tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc accggaaccc
     8581 ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg attaatgact
     8641 gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt cgcttctggg
     8701 aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc cgcaatggga
     8761 ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa
     8821 cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc
     8881 gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt attggatcca
     8941 ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta tggggaactg
     9001 gtcaaggata gtctggatat tttagcagaa gacgggatgc ttattgcgtc tacaaatgct
     9061 gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca tgccaaaaat
     9121 gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac gacggatacc
     9181 tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag
     9241 gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag accaaagatt
     9301 tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt tcttgcacgt
     9361 gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt tttttctttt
     9421 gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact gccgttatta
     9481 tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc
     9541 gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat cgagttgttc
     9601 aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa cattccttta
     9661 attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct ttaccgattg
     9721 aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc caataatgaa
     9781 cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt ctccatgcct
     9841 attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg cggaacgacg
     9901 ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc aggacaatta
     9961 ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta gaacaagttt
    10021 gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga
    10081 acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc acctaagtgc
    10141 gtcatcggtc gctgaacagc ttccagaaag ccaatctcgt atatttaaaa cactggttgc
    10201 tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg aattgaatct
    10261 aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt taaaagattt
    10321 ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga aaaaactatt
    10381 tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct cggctggcag
    10441 aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga gcggacaatt
    10501 tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg gatgttgttt
    10561 gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt cattcctgct
    10621 acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc attgacatcc
    10681 agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt tttaggataa
    10741 atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg aaaagttttg
    10801 ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg tttttgagag
    10861 gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac taacaatcgc
    10921 tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt acttttttct
    10981 gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc
    11041 actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg cagtgcagcc
    11101 ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt caatacacga
    11161 ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg aagtgttttc
    11221 tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag aggcgtcacc
    11281 gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt agatggataa
    11341 gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa ttccgtgcgt
    11401 ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga gaatagaagc
    11461 gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt tgatttattt
    11521 gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg tgattcaaag
    11581 aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa ctttccattt
    11641 ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt gcaccgacca
    11701 ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt
    11761 gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc gccgactacg
    11821 atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat atctgaagaa
    11881 agaaaacaga aaaaaaatgt tttgagccaa aagagaaaat gagctaaaat gagggaagat
    11941 aagcaaagga gcgtggaacg atgacaaacg caacagaata tgttcaagca attcaagaaa
    12001 agctacatca aaaggatcaa gggcaagaag aatttttaca agcaatcgat gaattcatgc
    12061 ccacggttat gtcttttttg gacactcatc ccgaatatat cgaaaagaat atcttagaac
    12121 ttttaacgga accagaaaga gttattcaat ttcgagtgcc ttggcaagat gatgaaggaa
    12181 actggcgagt aaatagaggg taccgtatcc aatacaactc agctatcggc ccttataaag
    12241 gtggattgcg cttccatcct agtgtcaatc taagtatctt gaaatttttg gcttttgaac
    12301 agattttcaa gaatagttta accggtctgc caatcggtgg aggaaaaggc ggaagtgact
    12361 tcgatcctaa aggaaaatct gataatgaga tcatgcgttt ttgtcaaagt ttcatgctgg
    12421 aattgtcaaa acatatcggc ccatcgattg atgttcctgc gggagatatc ggtgtaggag
    12481 ctagagaaat cggctatttg ttcggcgaat ataaacgatt gaaaaaatat gatactggtg
    12541 tgttgacagg taaacctttg gatttttggg gaagtaagat acgtacggaa gctactggtt
    12601 atggattagt gtattatgtc aaacatctat tgaacgaaga aaaagattca tttaatcaaa
    12661 aaacagtttt tgtttccgga agcgggaatg tagccattta tgcaatcgaa aaagtgcaag
    12721 agctaggcgg gaaagttgtt acatgttcag attctagcgg gtacatctat gatccagaag
    12781 gaatagatgt gaaactattg aaagaagtca aagaaatcaa tagacaacgt ttgacggaat
    12841 acgcaaacca acgtccttct gctatttatc atgctggaga atctgtttgg acattgaaag
    12901 aaaaagcgga tatcgctttg ccatgtgcta ctcaaaatga aatagacgaa agcttggccg
    12961 aaatcttagt agaaaatggg atcaaaatcg tagcagaagg cgctaatatg ccatgtacag
    13021 taaaagctgt ttctgttctt catgaagcag gcgtgtggta ttgtccagga aaagctgcta
    13081 atgctggagg agtagcagta tctgctttag aaatgagtca gaacgtccag cgattgactt
    13141 gggaaagaga gcaagtggat gagcagctgg atcaaatcat ggaaacgatt taccatacgt
    13201 gtcgccaaac agcagccgag tacggcgacg aaaaaaatct gcttcttgga gcaaatgttg
    13261 ctggatttga aaaagttgcc aaagcaatgg cagcagaagg tttagtttga aaaatgaaac
    13321 tttcttggaa atattgtcag cattttgttt atttgcttga aaaataataa aaaaaaacgt
    13381 aaagtattac ctgtagagta aacctataag gaggagaaca aatggcacac attcattttg
    13441 actattcaaa agtagcaccg tttgtcaacg aacaagagtt agggtacatg caaagccaag
    13501 taacagcagc ccacaacgaa ttacgggaag gtactggcgc aggaagcgac ttccgcggct
    13561 ggatcgattt accaacgaac tatgacaaag aagaatttgc acgtatcaaa gaagcagcta
    13621 aaaaaatcca atctgattct gaagtattag ttgttatcgg tatcggtggt tcttatttag
    13681 gcgctcgtgc agcgatcgac ttcttgaatc acacattctt caatttatta gatagcaaca
    13741 caagaaaagc accacaaatc ttctttgccg gaaattcaat cagctcaaca tatattgctg
    13801 acttgatcga agtaatcggt gatcgcgact tttcagtaaa cgttatttca aaatctggaa
    13861 caactactga accagcgatt gcgttccgtg tattcaaaga gcttcttgtt aagaaatatg
    13921 gacaagaaga agcaaacaaa cgtatctacg ctacaacaga taaagcaaaa ggtgcagtta
    13981 aagtagaagc agatgcagaa ggttgggaaa ctttcgtgat tcctgatgac atcggcggac
    14041 gtttctctgt attaactgct gttggtttgt taccaatcgc tgttagtggt gcagatatcg
    14101 atgcattgat gcaaggtgcc gctgatgcaa gcaaagcata ctcaagcgac aaactagaag
    14161 aaaacgaagc ttaccaatac gcagcaatgc gcaacattct ttaccgtaaa ggcaaagtaa
    14221 ctgagttgtt gatcaactat gaaccaggaa tgcaatactt ctcagaatgg tggaaacaat
    14281 tgtatggtga atctgaagga aaagaccaaa aaggcatcta tccttcaagc gcaaacttct
    14341 caactgattt gcactcttta ggacaattca tccaagaagg acgtcgcaat atttttgaaa
    14401 cagttgtgaa ggttgaaaaa cctcgtaaat caatcacaat tcctgaacaa gcagaagatt
    14461 tagacggtct aggctacttg caaggaaaag aagtggactt cgtaaataca aaagcatttg
    14521 aaggaacatt gcttgctcat acagacggtg acgtgcctaa cttgctagtc aaaattccag
    14581 aaatggatgc ttatacattg ggttatacaa tgtacttctt tgaaattgct gtaggtattt
    14641 caggttactt gaatggaatc aacccattcg accaaccagg tgtagaagct tacaagaaaa
    14701 acatgttcgc acttctaggc aaaccaggct ttgaagatct tgctaaagaa ttgaacgaac
    14761 gcctgtaaga taagggtttt tcgctagatt taaataatca gtgatagaaa catgttcgct
    14821 ttaaaatgaa aactggtacc catttaggta ctcaaacata ctgcatcgtt tattcagaat
    14881 aaggggctgt gacaagacgt ttgtcacagt cccttgtttg tatagaaatc gattgattgc
    14941 cttgctcttt agtttgtttt aggactttaa gaacagtgag gctgacttcg aaggttaatt
    15001 ctgttttttt gttttctaga atcagtaagc aaaaaagcac tcacttcgag tgcttttttg
    15061 cgatatattc ttagcttaag ataggcctaa tttttctttt acagatgagg gtaccttatc
    15121 gctggtaatt acttttttct ctatgatccg cttttcgctt gctttcaact gcaagatcgt
    15181 tccttcgggg taagcagaac cattggcaga gtcaccagaa gtaatgacat cgaaatctaa
    15241 ctgtcttttt tcaccgtttt cagcataagc atctacgttg tatttaaaac ctcttccaat
    15301 tacttcccca ttatctgctt tgatatcagt ttctgcaggc attggagctt tgactaccgc
    15361 ataataatct ttacctacat aagtagactg atagtagtcc caacctttcc atcctataaa
    15421 agcaatcaat atgataacga tcaaccctaa aatttttttc atgaaagatc tcctttttta
    15481 tttttattat acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc
    15541 ttcaaaacgt cgttgtgctc gacttaatag aacactgtaa gccagtaata tgctttcaaa
    15601 tctactctct agccctttaa cagaacgtga gttgaaattt tgacaaccta atttttctaa
    15661 ggaagaaaaa acagtctcaa ctg
//
LOCUS       DAPCMT010000059        15515 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPCMT010000059 DAPCMT010000000
VERSION     DAPCMT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.059"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            138..1475
                     /locus_tag="RD743_001551"
     CDS             138..1475
                     /locus_tag="RD743_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDU0563367.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="RD743_001552"
     CDS             1523..3652
                     /locus_tag="RD743_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDU0563368.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="RD743_001553"
     CDS             3627..4316
                     /locus_tag="RD743_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563369.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="RD743_001554"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="RD743_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="RD743_001555"
     CDS             complement(5546..5740)
                     /locus_tag="RD743_001555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563370.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="RD743_001556"
     CDS             complement(5790..6230)
                     /locus_tag="RD743_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDU0563371.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="RD743_001557"
     CDS             complement(6234..7079)
                     /locus_tag="RD743_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDU0563372.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="RD743_001558"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="RD743_001558"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDU0563373.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="RD743_001559"
     CDS             complement(8703..9650)
                     /locus_tag="RD743_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDU0563374.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="RD743_001560"
     CDS             complement(9873..10223)
                     /locus_tag="RD743_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDU0563375.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="RD743_001561"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="RD743_001561"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDU0563376.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="RD743_001562"
     CDS             11646..11966
                     /locus_tag="RD743_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDU0563377.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="RD743_001563"
     CDS             11988..12452
                     /locus_tag="RD743_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDU0563378.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="RD743_001564"
     CDS             12657..13262
                     /locus_tag="RD743_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDU0563379.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="RD743_001565"
     CDS             complement(13301..14590)
                     /locus_tag="RD743_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDU0563380.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="RD743_001566"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="RD743_001566"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDU0563381.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4843 a         2899 c         3182 g         4591 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaa
//
LOCUS       DAPCMT010000060        14968 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPCMT010000060 DAPCMT010000000
VERSION     DAPCMT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.060"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..761
                     /locus_tag="RD743_001567"
     CDS             <1..761
                     /locus_tag="RD743_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0563382.1"
                     /translation="NTVIHYHAFIRKALQEAVITELISTNVADKVRKPKKKQFVSQVY
                     NQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVIIEN
                     PENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILEDNEYV
                     FTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEWLG
                     HASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1335..1904
                     /locus_tag="RD743_001568"
     CDS             1335..1904
                     /locus_tag="RD743_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDU0563383.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1891..2751
                     /locus_tag="RD743_001569"
     CDS             1891..2751
                     /locus_tag="RD743_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDU0563384.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2941..3645
                     /locus_tag="RD743_001570"
     CDS             2941..3645
                     /locus_tag="RD743_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0563385.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3650..5485
                     /gene="walK"
                     /locus_tag="RD743_001571"
     CDS             3650..5485
                     /gene="walK"
                     /locus_tag="RD743_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDU0563386.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5482..6798
                     /locus_tag="RD743_001572"
     CDS             5482..6798
                     /locus_tag="RD743_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563387.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6799..7668
                     /locus_tag="RD743_001573"
     CDS             6799..7668
                     /locus_tag="RD743_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDU0563388.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7723..8532
                     /locus_tag="RD743_001574"
     CDS             7723..8532
                     /locus_tag="RD743_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDU0563389.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8635..9264)
                     /locus_tag="RD743_001575"
     CDS             complement(8635..9264)
                     /locus_tag="RD743_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDU0563390.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9434..10726
                     /locus_tag="RD743_001576"
     CDS             9434..10726
                     /locus_tag="RD743_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0563391.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10802..11464)
                     /locus_tag="RD743_001577"
     CDS             complement(10802..11464)
                     /locus_tag="RD743_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDU0563392.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11689..11973
                     /gene="groES"
                     /locus_tag="RD743_001578"
     CDS             11689..11973
                     /gene="groES"
                     /locus_tag="RD743_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDU0563393.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12024..13649
                     /gene="groL"
                     /locus_tag="RD743_001579"
     CDS             12024..13649
                     /gene="groL"
                     /locus_tag="RD743_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDU0563394.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13738..14886)
                     /locus_tag="RD743_001580"
     CDS             complement(13738..14886)
                     /locus_tag="RD743_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0563395.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
BASE COUNT         4940 a         2547 c         3139 g         4342 t
ORIGIN      
        1 caaatacggt tattcattat cacgcattta tacgcaaagc tcttcaagag gctgttatta
       61 cagaattgat aagtacaaat gtagcagata aagtaagaaa gccaaaaaag aaacaatttg
      121 tttctcaagt ttataatcaa caagagatct tgaaattact tgacattttg tcagaagaaa
      181 aactctattt ggtagttctt ttaacagcat tttatggatt gcgaagaagt gaagttttag
      241 gattaaaatg gtctgctatt gattttttaa acaaaaaaat catgataaac catgtaatca
      301 tagaaaatcc agagaatatg tcacagttaa taaagaaaga tcaaacaaag aactcttcca
      361 gctatcgtac attacctttg gttgaaacga ttgaacgagc attagtaaat caagctaaat
      421 ggcaacaaga taatcgtatt cttttaggag atgattatat tttagaagat aatgagtacg
      481 tatttactat ggaagatgga cggttgatga agcctcaata tgtaacgcat cgtttgagta
      541 agttgattaa gaaaaatgga ttgaaaaaaa ttcgctttca tgacttgcgt cattctaatg
      601 cttcaataat gttagatagt ggccagaata tgaaatccat tcaagaatgg ctgggacatg
      661 catcgtattc gacaactgct aatttataca cgcatttaac cgcaggagta aaagatcgtg
      721 cagcagaagc attagaaaac gcatttgggt ttaaagaata aaaaaacaaa aaacaatatc
      781 tttggggtat cgcttcttgt ttttagaatt tttttgttgg caatttgttg gcagtatgtt
      841 ggcaatgaaa aaattcacaa actaaaaacc tatttataaa gctttataaa acttttttca
      901 caaatagagc atttggttaa gagtcagctg ctctaccaac tgagctaacg gcccgtacaa
      961 aaagtactta ataatagtag cactaagaat tttaaaatgc aagactacat ttaaagaaaa
     1021 tcagaaaaat ctttacttcc ttataggaaa tcattcatag attattcaca tttgctcgct
     1081 attatataag cataccaaca aacaaagttc ttgcaagaga acaaacccta acactttttt
     1141 cattttttac tcctttcccg ccgtctcccc ggcgggtctc ctgttcttaa cgataaaaga
     1201 caagaaggct tagccttctt gttttttgtt ttcatggaat cccgctaatc aaatcaccta
     1261 gttaaagcaa ctagattgaa tgtttatcat aagtctgata tcatgtttat gaaatgaaaa
     1321 aaggagcggt tatgatggaa aaaatgcgtc agcaattaat ggagtggggg aaggaactag
     1381 aaacattccg tctgcctcac tgggaagaac tccctgactt agaactttat atggaccaag
     1441 tcagaacgtt agttgatcgt tatctttcac ctgtgataca aggagaaaag catccattgc
     1501 ttacctcttc tatggtaaac aattacgtta aactaggttt gataccagca ccagttaaaa
     1561 aaagatataa caaagaacac gtagcatttt tacttgcaat cactacgttg aaacaagtat
     1621 tgactattcc tgaaatcaaa gaagggatat tgttccaagg aaaaacagta ggaattcgtg
     1681 aagcctataa tctattttgc gatgaacaag aagcagctgt gtggatggtc agccaattag
     1741 cacaaggtaa atctcatccg caaaaattcg accaggcaac ccctgtagaa tatattgctg
     1801 taagagcggc aactttatcg tttgcaatga aacttcttgc ggaaaaaacg attgttctag
     1861 aaaccgaata tttaaaagag gagaaaacaa atgaaaaaca ataaaatcgc aattttagta
     1921 gattcaggta cggatgttcc agaagaattg ataaaaaaat acgagatgta tatgatccca
     1981 ttgaaaatta tttataaaga tcaggtatat acagataaaa ttgacattac ccctgaagag
     2041 atctatcaac gtttgccaga ggaaatccct ggtacatctt tgccggatgg tgagtcgatc
     2101 acagaaatct ttgagcaaat caaaaaagac ggttatgaaa aggtacttgc ggtaacaatt
     2161 tccagtggat tgagcgggac atataatgtg gttcgtttaa ttgcacaaca gcaagaagaa
     2221 ttggaagcat atgtactaga tacgaaaaat attggtattg gtgcaggttt ttctgcgatc
     2281 caagcagcaa aatggctgga agaaggagta gaatgggatc aactgatcag taatctgaat
     2341 gagttagtcg aaagaacaaa agttttcttt aatgttgcga cattggaata tcttcaaaaa
     2401 ggtggacgga tcggtttagt tgcttctatt gtaggaaccg ctttaaagct taacccaatc
     2461 atctcatgta atgaagaagg gatttattac acagtaggaa aagctagggg acgtaaaaaa
     2521 agtttggata aaacggttgc ttacgtcaaa gaaaaaatcg gtaatgcaaa ggtcttcaac
     2581 ttagctgttg cccatgggga tgcgaaagaa gaagcaattg aaatggaagc gagacttaaa
     2641 agagagttcc cacaagcaaa ccaaatctac tttggccaga tttctccggc attagtagtc
     2701 cacactggtc cgggattatt gggtgtcggt gtacaagttt tagaaggata aatcaaaaaa
     2761 aggaaacact gaggtgaaaa tcagtgtttt ccttttttat tgaagttaga acaaaaaata
     2821 ataaagtatt ttgcactttc attttaattt tctcttttgt tttataggca aagatgaaac
     2881 aaaagcagaa atacggtata atgagaaggt atgacaataa tatataagag gtggctcttt
     2941 atgaaaaaag ttttagttgt cgatgatgag aagccgattt cggatatcgt caaattcaat
     3001 ttagccaaag aaggctatga cgtttatact gcatatgatg gggaagaagc cttggaaaaa
     3061 gttgctgagg tagaacctga tttgatttta cttgatttga tgctacctaa aatggacggc
     3121 ttagaagttg ctcgtgaggt acgcaaaact tatgatatgc cgatcattat ggtaacagcc
     3181 aaagattctg aaattgataa agtcctaggc ttagagttag gcgccgatga ttacgtgacc
     3241 aagccatttt ccaaccgaga attagttgca cgtgtcaaag caaatcttcg tcgcggtgcc
     3301 acggctgcga aggaaccaga agaggcagca cctgcggaat tgacgattgg tgatttgacg
     3361 atccatccag aagcttacat ggtgacgaaa cgaggagaaa caatcgagtt aacacatcgt
     3421 gaattcgaac tactctttta cttggcgaag caccttggac aagtgatgac aagagaacat
     3481 ttattacaaa cggtttgggg ttatgattat tttggagatg tacgaacagt ggatgtaact
     3541 gtacgtcgtt tgcgtgaaaa gatcgaagat aatccaagcc atccaaatta tttagtgaca
     3601 cgtcgtggtg tcggttacta tttgcgtaac ccagaacagg agtaattgga tgaaaagaaa
     3661 agtccgcttt tttcgatcag tcaattttaa gatcgctatt acctttattc tgattttatt
     3721 gatctcgatc gaaattattg gtgcctactt tatacgtggt ttagaacgtt ccacgatcaa
     3781 tacatttatt aaagatatga atcaaacggt ggaatcatta gcaacaacaa tcagcccgga
     3841 attgaatcga aaagataatg cagatgatga agaggtaaat gcgaatatca aaagatttat
     3901 tgaaaacagt gcgactagcg atatcatcga aatacgggtc gttgacgaaa aagggatcat
     3961 ccgaggaaca accgatgtca atgaacaaag cgctgtggga aagaaaaatg attatgttga
     4021 tatcaatgat ttcacgacta aacgttatgt tgctttagat aacgacgata aacgggtgaa
     4081 tatcaatgtt caaccgatcc tgtccccgac cggcgatgcc gttatcgggg ctctttacgt
     4141 caaaagtaat atcgaacaaa aatattcgga aatcaataac acagcagtca ttttctttac
     4201 tgcctcacta attgcggcat ttatttcaat gatcgtatct gttttagttg ctcgttcgat
     4261 tacacagccg atcggtgaaa tgagagaaca agccattcga atcgctagag gagactacag
     4321 tcggaaagtc aaagtgcatg gacaagatga acttggacag ctggctgata ccttcaatca
     4381 gttagctgaa cgtattgaag aagctcaaga tacaatggaa gctgaacgca atcgtttgga
     4441 tagcgtgcta tcccatatga cagacggcgt cattgcgaca gatcgcaggg gtaaagtcat
     4501 tacgatcaat gatatggctg tttccttgct agatatcaaa aacgaggaag caatcggaca
     4561 atcgattttg acacttttag atatcgaaga agaatatacg ttgcggaaat tattagaaga
     4621 tccaaacgaa atggtacttg accgttcaac cagtacattg gaaagtgatc agatgatctt
     4681 gcgagtagac ttttctatga tccgtcgcga atcaggtttt atcagcggat tagtagctgt
     4741 cttgcatgat gtgacagaac aagaaaaaac agaacgggaa cgtcgtgaat tcgtctctaa
     4801 tgtttcccat gaacttcgta caccgttgac aagtatgcgt agttatatcg aagcattgag
     4861 cgaaggtgcg tggaaagacg aggaagtagc accaaatttt ttgaaggtaa cgctagatga
     4921 aacagaccga atgattcgaa tgatcaatga cctcttaaat ctatctcgca tggatacagg
     4981 taatacccag cttcaattag aatatgtcaa tttcaacgag ttagtgaatt ttgttcttga
     5041 ccggtttgat atgatggtgg gaaatcagga aaaaaattat aagatccgtc gtgaatttac
     5101 gcaaagagat ctttgggtcg aagtagatac agataaaatc atccaagtag tcgataatat
     5161 catgaataac gcgatcaagt attcaccaga tgggggaacg attacttgtc gcttattaga
     5221 aacgcataat aacgtgattt taagtatcac cgatcaagga ttggggattc ctaaaaaaga
     5281 tctgaaccga gtgttcgagc ggttctatcg agtagataaa gcacgtgcaa gagcacaagg
     5341 tgggacagga cttggattag ctatttctag agaagtgatt aaggcgcatc gaggcgctat
     5401 ttgggcagag agcaaggaag gaaaagggtc gactttttat atctcgctgc catatgaacc
     5461 ttatgaggag gaatggtggg aatgagaatc tctgaaaaag tcgttcgtat aggactcatt
     5521 ttgatggttg cgttaagcat atacttctct tatgcaatct ggctgagccc agcaggaaaa
     5581 agttcaatca acgtagatga gacaaacact caaatgatcg agtctcaaag caatcgaaaa
     5641 acttccgaag tctttcttcc gcttcatgtt acatggcacc attcgggaat gatccaagaa
     5701 accaacagtg aaaatctcgt gtaccgattg caattgatca tcgataaagc aagatttgga
     5761 aagctttctg aagtagtttc aggagacaaa tcacagtttg aacaggtgaa aaatctgaac
     5821 tctggttttg agttggccta taatgcgcct tttttgctaa gagaatacaa ggaagcattt
     5881 gatttagatt tagatgtgtc gaacttggca aaagaaagcg aaaatatgta ttttactaga
     5941 atacaattcg atcaaggaaa taaaaaaatc cgatttttga attattcgaa ccatctcgta
     6001 tatgaagcaa atatttcgtt gaattggaca gatatcaata aagaattaac agcaaaagac
     6061 acaaaatgga cagaaatgac aacagaacca tcgacgatcg acacgcaata tgctacgaaa
     6121 cattcgatca aactgaagaa gtatagttat atcctgtcac agcaacccta tacacttttt
     6181 agaaatgctt ttttccagaa accagatagc gtgaaaaata atgatgaaag caatgaattg
     6241 attttttacg gtgggaatga aacaatgtct atccattcgg aaacgcaagt agttgatttt
     6301 cgtggagagc tgccagagaa aaaagaaaat gctagtgtat tttctgaaag tttcccctat
     6361 attagcaaat taggcagttc tttagggaac ctcagatatt ttgaccgcag tgacaatacg
     6421 atcgattatc ggatattcgt ggaaggcttc ccggtttttg ggacagacag cagaggacag
     6481 attggtgtgg aagttggcgg tgaaacgaat ggttctgtgc aagtgaatat ccaaaccagt
     6541 ttgaatacga ttcaagtgcc gataccttca gacgaagaag tagaacttgc aagtacgaat
     6601 gaaatcatcc agcagttaaa agctaaggga gcagatagca acaagattgg ttcagtgatc
     6661 gttggctata catggcaaaa tgtcaaagaa acgaatcgtg tcgttgatct cttacctgaa
     6721 tggtatgtga aatataaaga taactggtat tcagctaacg accttctgga aaaattacct
     6781 ggattggagg cgaattaatt ggatttcaaa aaaatcgaat ggatattttt cgtcgctttt
     6841 ttagggttga atgtgttttt attcagcagt tatcatgacg cggtatacca agaaaacaat
     6901 gtcattcgct ctaatcagac tgaatcaatc caacaacgtc tggctagcga cgatatcact
     6961 tacacgggga agatttcgtc tgagaacaag caaggctatt atcttagcgc ggagcagacc
     7021 aatcttagca cttcattagc gaattatcgc aaggctggta aaagcggctt gctggcaagt
     7081 ggtgtgtttt tagacggtga tgttttgacg aaatctttat ccagttcaga tgctgaaaag
     7141 aacgtgatcg atccagataa aattagtagt tccttggaag attttttatc taacaaagac
     7201 aatattttat tcggtaatga ttatgtgtac acgaaaaact tttctcattt agaagaagaa
     7261 ccactagaaa tccaagcaag ccaagagtat gaaggcatcc cattcaatga tgatacagca
     7321 aaaatcgttc tttctttaga aaaaaacgat aatgattata gcatcacgaa atacatgcag
     7381 acgcatttat ctgatatcga gcagctgcgc gaaaagacgg aactgtatac ggaagaggaa
     7441 gcaatcaata cattgtacat caataacaaa ataccaagag gatcaaaaat tttatggcgt
     7501 cagctagctt attcctgtat tctgaaagtc cgggaaaaaa atgtgtatgt tcctgtttgg
     7561 catgtagcga tcgaaacaag cgataaagtg gtccaaattg agagcgtcaa cgctttcagc
     7621 aatacgatcg tgacaaacaa tactgtacca aaagtggaag atcaataaaa aagcgagtat
     7681 aatgaaaaaa gtttacaaag taagaaggga agtaggagtc aaatggctga agaaaaacca
     7741 gcttttaaaa tcagtatcct agcaagtgga agttcgggta attcacttta tatcgaaagt
     7801 gaaaaaaaac ggcttttggt cgatgcagga cttagcggta aaaaaatcac ttccttgatg
     7861 gctcaaattg atcggacacc ggaccaatta gatggaatac ttgtgacgca tgaacatcga
     7921 gatcatatac atggcgtggg tgtcttggct cgtaagtatc atttggatgt ttttgccaat
     7981 cagcagactt gggaagcgat ggaaccactg attggaaatg taccagtgga acaaaaacat
     8041 ctatttgaga tggggaaagt cagaacattc ggcgatatcg atattgagag tttcggcgtc
     8101 tcccatgacg cagcggctcc gcaattttat cgtttccata aagaaaatcg gtcattcgtc
     8161 atgttgacgg acaccggata ttgcagtgat tatatgcgag gtgtcattga aaatgccgat
     8221 ggttatttga tggaaagtaa tcatgatctt gagatgttac gcatgggacc ttatccgtgg
     8281 aatttaaaac agcgtatttt aggagataga ggacatcttt ccaacgaaga cggtgcactt
     8341 gtcatgactg atgtgatcgg taatcggacc aaacgaatct atcttggcca tcttagcaag
     8401 gaaaacaata tgaaagaact tgctcattta acaatggaaa atgttttgaa agaaaaagat
     8461 tttggcgttg gacatgattt tgaaatctat gatacagatc cagatgaagc aactgagcta
     8521 ttttcgattt aaacagttca atcaataaaa atataaaaag cagaagataa aatcaatcag
     8581 ggttggtttt atcttctatt tttatttttt tagcatatta aaaaaataac cgtattatcg
     8641 actgactaca ctgatgtgct gcttttcatg tttctcatcc agtatctcat ctactaatgc
     8701 aatcgcgtaa tctgccatgc tgatcgtact gtcgctagca gcgtttcttt gtaaataatc
     8761 atcacttaac aaataagtac ctgtacgttt gccatgtgga tcaaataaag aagctggact
     8821 gatataagtc caatgtagat ttttagattg tttcaatttt aacaatcctt cgtacatatt
     8881 gaaagctgtt ggatagaagg gtgcattttt aggtacttgg tcaatcatac gagtcgtctt
     8941 ttctgtatct aagaataacg aagaggcacc cccaacgaca attaagcgtg tatcggttcc
     9001 ttcaagcatc tcaatcaaat gggacaaaga agtttgatgc atttcttctt ttcctttggg
     9061 agcgttgaaa gcatcaacga caacatcaaa agatgcaaga tcagtagttg tcaactcaaa
     9121 taaatccttt tgtataaacg gaacatctga tggcaatttt tctggatgac gcacgattgc
     9181 cgtcacgtct aaatctctgg acaatgcttc gtctaaaata aatgaacctg catggccagt
     9241 agctccaatg agtgcaattt tcataaaaaa cctccttttt tattcctgtc cattgtaagc
     9301 agttgctgaa tattcgtcaa agaatccgat ttttgaaaag ctcagtgcgc attcctttca
     9361 tggttcgtga tcattgggta tagttaaatt aagaaaactg attgataaaa atagaagttg
     9421 aaggagataa gaaatgaaag agtggttaga tcgtgccttt ttaccaatga taacatctgt
     9481 tgtgaatctt agacccttaa ctgctttgcg taatggaatg acttacacga tcccaataat
     9541 tattgttggc tcgctgtttt tcttatttgc tcattttcct gtggattaca tcgctcagtg
     9601 gatagacaaa acaggatttt ctatgtattt tacacaagta taccaggcta catttgatgt
     9661 gatagccttt tggatcgttt ttgccattgc atacgcttat gcaaaagaag aagacatcga
     9721 tcgcttacca gtcggcataa cagccttgtc tgcttatcta ctgcttattc agccactaaa
     9781 tactcagata gatactgtct ggacaggtgc tgaagggata ctaggagcag ttacaactgg
     9841 gctcatcatc ggatggggct atgcacgttt agtaagactt gcgaaagggt ggaagcaaag
     9901 agagaagata cctgaaaatg tttggcagtc ttttacttca ttgattccaa tcattgttat
     9961 agtcactttt acgcttatcg gttcagcggt ttttcaacat ttgttcagaa tgactgttgt
    10021 tcaaggagta tggacatatg tccagcttcc cattcaatcg ttcatagaca cgttgtttgg
    10081 tgtgctttta ttaggttttt ttgttccgtt tttatggtta tttggtcttc atggatctgc
    10141 gatggtcaat gggttggtta gtcccgtctt gcaagcgaat tcattagcaa atgcagaaat
    10201 cttagcctca ggacaagagt tgacggtggc aaatggcgga catatcgtta ctcagcaatt
    10261 tctcgatcag ttcatgacgg ttacgggtgc tggattgacg ttaggggctg tattttttat
    10321 gacggttttt gcgaaatcga agaaatacag agaattagga aatcttgctc ttttaccagc
    10381 ctttttcaac atcaatgaat caattatttt ttccacaccg atcgtgatga acccgatgat
    10441 ggctgttcct tttattttcg caccggtcgt ttcaggattg attacctatt ccgctttata
    10501 ttttggattc gttcctttat ttacagcagt ccaagtccct tggacaacac cgccgatcct
    10561 ctcgggtttc ttagtaggcg gagtgcctgc agcgatttta caaggtatcg tgttgtcaat
    10621 tagttttttc atttatttcc catttttgaa aaaaatagat tctgcaaact gcaaaaagga
    10681 aagacagaca aaaaatgatg gagcagcaga aaagatgaac gactaaaaaa caaaaatact
    10741 cctgtcgctt tagattggga aaatcaccaa tctaggatga caggagtatt tatagtcatg
    10801 tttatactag ccctttttgg acagctaatt gaacaagaac aactaatgtg ttcattccta
    10861 cgtgtgtgat catcgatgtc cagatccgac cagttgcttt gtactctagg ctgaagaaga
    10921 aacctaggaa aaagtagatt agcagatgac catcattatg agcaaacgca aacagcaaag
    10981 agctgatcac tgctccgacc caaaaatttg aatatcgact gatgatcccc agtatactgc
    11041 ggcggaatac gaactcttcc atgatgggtc cagcaacagt tgtcgccaaa ataaaagcgg
    11101 gtgcttccat gatcatctgg atgatatttt gtgtattctc ggaaggggtt gctttgccaa
    11161 acaatacaga ttctaacatg acagcgatgc tttgcagaat caatgcgaca aagattccaa
    11221 ccaatccata aataacaatc tttggagcag aagctttttt taccgctgat tcgatcgcta
    11281 agggttcacg ttgcttaaaa taaaaaatag ctaggacaac ggcgcctagt atatacatga
    11341 ctgcagttgc tgtgatcaat tcagacgtag attttacaag tcctatcgaa gctaggatca
    11401 agggtgagat aaaaattaga ccatagcaaa aaatgctgag gaagctatat ttttttaaag
    11461 acatgtaatt cctcctttta agggataaca agcatgcaag gcaagcttat atcgattgat
    11521 ttcaattata tcataatttc aataagcagc tgtcagaata aaatttcttt tctttgattt
    11581 aaaagcttgc aaaaaagagg gagaatgagt attattaaaa atgtaggtta gcactcgaag
    11641 gttaagagtg ctaacagatt aaaaaaagag ttatcggagg gatttatcgt gttaaaacca
    11701 ttaggtgatc gcgtcattat cgaagtcgca aaagaagaag aaaaaactgt cggaggaatc
    11761 gttttagctt ctgctgctaa agagaaaccc caaactggga cagttgttgc agtcggtgaa
    11821 ggccgtttgt tagaaaacgg tgaaaaagtt cctgccgctg taaaagcagg cgaccaagtt
    11881 atgttcgaaa aatacgccgg tacagaagta aaatacgaag gaaaagaata tctgatcgtc
    11941 gcaggaaaag atatcatggc gattgttgag taaatttgga agataaaaaa ataatcaaaa
    12001 ataatcaatg aggtgaatca atcatggcaa aagaattgaa atttgcagaa gacgcacgcg
    12061 cagctatgtt acgcggggta gataaattag cagatacagt gaaagtaaca ttaggaccta
    12121 aaggccgaaa tgtcgtacta gaaaaatctt acggctctcc attgatcaca aatgatggtg
    12181 tgacgatcgc aaaagaaatc gaattagaag atcattttga aaacatgggt gcaaaacttg
    12241 tatcagaagt agcatcaaaa acaaatgata ttgcaggaga cggtacaacg acagcaactg
    12301 ttctgacaca agcaatcgtt cgcgaaggtt taaaaaacgt aactgcaggt gctaatcctc
    12361 taggcattcg tcgcgggatc gaattggcaa caaaagcagc agtcgaagaa ttgcataata
    12421 tctcaactgt tgttgattca aaagaagcca ttgcacaagt agcagctgtt tcttcgggtt
    12481 ctgataaagt tggacatttg attgctgatg caatggaaaa agttggaaac gacggtgtca
    12541 tcacaatcga agaatcaaaa gggatcgaaa cagaactaga tgttgtagaa ggtatgcagt
    12601 ttgatcgtgg ttacttatca caatatatgg taacagataa cgacaaaatg gaagctgttc
    12661 tagaaaaccc atatatcttg atcacagaca aaaaaatctc taacatccaa gatattctgc
    12721 cattgttaga acaaatcttg caacaatcac gtccattgtt gattattgct gacgatgtag
    12781 atggtgaagc attgccaact cttgtattga acaaaatccg tggaacattc aacgtagtag
    12841 ctgtaaaagc tcctggtttc ggcgatcgtc gtaaagcaat gcttgaagat atcgctattt
    12901 tgacaggtgg tacagtaatt actgacgatc taggtttaga attgaaagat gcaacaatcg
    12961 aaaacttagg aaatgcttct aaagttgttg tagataaaga caatacaacg atcgtagaag
    13021 gttctggaga aaaagaagca atcgaagctc gtgtgcaatt gatcaaaaac caaattgctg
    13081 aaacaacttc tgattttgac cgcgaaaaat tacaagaacg cttagctaaa ttagcaggtg
    13141 gtgtagctgt tgttaaagtt ggtgcagcta ctgaaacaga attgaaagaa ttaaaattac
    13201 gcattgaaga tgcattgaat gctacacgtg ccgcagtaga agaaggaatg gtctcaggtg
    13261 gtggtacagc tctagtgaac gttatcagca aagtttctgc tgtagaagca gaaggagacg
    13321 tggcaacagg tatcaagatc gttgttcgtg ctttagaaga accaattcgt caaatcgctg
    13381 aaaacgctgg ttatgaagga tcagtgattg ttgataaatt gaagaacgtg gaactaggca
    13441 ctggtttcaa tgctgctact ggagaatggg taaacatggt tgaagcaggt atcgtagacc
    13501 caactaaagt aacacgttct gccttgcaaa atgccgcttc cgtttctgcc ttgttattaa
    13561 cgacagaagc agtcgttgca gataaaccag aaccagcagc gccagcagct cctgcaatgg
    13621 atccatcaat gatgggcggt atgatgtagt aattaagaaa gaaccctatt ttaacagggt
    13681 tctttcttct ttttttgttt tattttaagc aaaaggggca aagaaggggc aaaaatatca
    13741 aaggctattt agtttatcaa ttacttgttg tttggctctt tttgttacat ggttataaat
    13801 agacagagtt gtgtttgcat cagaatgccc tacacgctcc ataatggctt taagaggtac
    13861 gcctaattca gataatagtg aaacgtggct atgtctgaat atatgtgagg atagactttt
    13921 ttctaattcc aactcttcct ctactttatg gagtatagcg ttaaatgagt gcagcgcaag
    13981 cggagtgcca cttgtagata taaatatata ttgatcggga tctgtgggtc tacctgcaag
    14041 aatattgtca gctatcacac tttcaatcaa ttcttttgca cgattgggta attgcacttc
    14101 acgttgcgaa taagtatttt ttggagttgt ttttatagca ttatccattt tcacagatgt
    14161 gtaatctaag gtcccattaa tggaaatttt cccatcctca tagtccttca tttgcaaagc
    14221 tagcaattcc ccatatctca aaccagttaa atatagaaac tcagctatta tgccgtgtag
    14281 ttttctgcga ggattggagt atagctgttt cagtatttga tcaatttctt ctttctccag
    14341 atatttttta ttcatagaaa gccttctttt ttcttcttcc acttttttag gatggatttt
    14401 aactgctagc gcagggtttc tttgaatgta ttttctatcg attgcatagt ttagcataac
    14461 agatagagtt gtttttgttt gttttgtgta gttcagtgag aggtcaccaa acgtatacat
    14521 atcttcaatt atcttattaa tgagtgtctc atcaatgttt ctaacgatcg tatcatcgct
    14581 tatgtgctta gaaacatgtt tcatcatcat tggaaccttc aaatagctag tacgtttaac
    14641 atgctgctta taatattcat accattcttt atacagctca ccaaaagtga tatctgattt
    14701 attgtaatct tcgagtgctt ctttaatttt tttatctaga attttctgag cttttttcca
    14761 cgcttgtggt gaattacttg taagtgttgt agattttttt cgtgtttttt ctgtataagg
    14821 atctacatat ctttcaataa acttgaattg gccatctttg gtctgttcaa cccacacttt
    14881 taacatctcc tatcatttgc tataataggc ataacaaata gacctatata ggtttgtttt
    14941 ctaaaagcac gctcttactc tggacggt
//
LOCUS       DAPCMT010000061        14815 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPCMT010000061 DAPCMT010000000
VERSION     DAPCMT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.061"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(296..1024)
                     /locus_tag="RD743_001581"
     CDS             complement(296..1024)
                     /locus_tag="RD743_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDU0563396.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="RD743_001582"
     CDS             complement(1002..2576)
                     /locus_tag="RD743_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDU0563397.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="RD743_001583"
     CDS             2805..3743
                     /locus_tag="RD743_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0563398.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="RD743_001584"
     CDS             3755..4666
                     /locus_tag="RD743_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0563399.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="RD743_001585"
     CDS             4690..6162
                     /locus_tag="RD743_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDU0563400.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="RD743_001586"
     CDS             6213..6824
                     /locus_tag="RD743_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDU0563401.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="RD743_001587"
     CDS             6854..8767
                     /locus_tag="RD743_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HDU0563402.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="RD743_001588"
     CDS             8895..11876
                     /locus_tag="RD743_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HDU0563403.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="RD743_001589"
     CDS             11873..13534
                     /locus_tag="RD743_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HDU0563404.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="RD743_001590"
     CDS             13574..14506
                     /locus_tag="RD743_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDU0563405.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAPCMT010000062        14688 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPCMT010000062 DAPCMT010000000
VERSION     DAPCMT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.062"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(13..726)
                     /locus_tag="RD743_001591"
     CDS             complement(13..726)
                     /locus_tag="RD743_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0563406.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            901..2325
                     /locus_tag="RD743_001592"
     CDS             901..2325
                     /locus_tag="RD743_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0563407.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2624..3331
                     /locus_tag="RD743_001593"
     CDS             2624..3331
                     /locus_tag="RD743_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDU0563408.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3318..3641
                     /locus_tag="RD743_001594"
     CDS             3318..3641
                     /locus_tag="RD743_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDU0563409.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3730..4767)
                     /locus_tag="RD743_001595"
     CDS             complement(3730..4767)
                     /locus_tag="RD743_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDU0563410.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5088..6071)
                     /locus_tag="RD743_001596"
     CDS             complement(5088..6071)
                     /locus_tag="RD743_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDU0563411.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6486..7256)
                     /locus_tag="RD743_001597"
     CDS             complement(6486..7256)
                     /locus_tag="RD743_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0563412.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7249..8184)
                     /locus_tag="RD743_001598"
     CDS             complement(7249..8184)
                     /locus_tag="RD743_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0563413.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8500..10149)
                     /locus_tag="RD743_001599"
     CDS             complement(8500..10149)
                     /locus_tag="RD743_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDU0563414.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10266..12869)
                     /locus_tag="RD743_001600"
     CDS             complement(10266..12869)
                     /locus_tag="RD743_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDU0563415.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12881..13930)
                     /locus_tag="RD743_001601"
     CDS             complement(12881..13930)
                     /locus_tag="RD743_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDU0563416.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13956..14270)
                     /locus_tag="RD743_001602"
     CDS             complement(13956..14270)
                     /locus_tag="RD743_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDU0563417.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14286..>14688)
                     /locus_tag="RD743_001603"
     CDS             complement(14286..>14688)
                     /locus_tag="RD743_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0563418.1"
                     /translation="NASDKKSVVEKLSKLLETNGFVSDHKQFQKDVFDRENHMTTGIG
                     KNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTDKGTNHLRIL
                     AELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         4343 a         3051 c         2484 g         4810 t
ORIGIN      
        1 attttcccga gcttattttt caacaaaaac agcttcaaaa acaaagtctt gatataaatg
       61 tctcgatacc gaatactcga aaggtttacc agacgataga gcaacaactt tgtctatctc
      121 aatcataaat tcacctggat ctatttttaa tatttttgcg tcaaattcat tcgcagtatc
      181 tcctttgata cgtatgtgcg aagtctttat ttccagaccc agttcatttg tgatataagc
      241 atatacagat ttctttaaat gttttggttc caagcctggt atgactgcta atggcatgaa
      301 tgtttgctca atcgagtatg gttgacaatc aataaaacgg cttcgtacaa tattatatac
      361 atagtctttt acactgatac ttaatttatc agctactttt ttcgatggaa gttcaattga
      421 gaaagtatgg atttctgaat ctacgtgttc ttcaccaacc acgctaaaca aaccttcgtt
      481 tggaccaaga actcgtgagt tatcaattgg tgttctgacg acttcagtcc catacccact
      541 tctacttcgc aaaactcctt cggaaacaag taagtctacg cctttcttta ctgttaactt
      601 gcttgtatga taaataactg ccaattcatc tcctgtagga agtttctcac caattttata
      661 ctctccattt acaatttttt tctttatatc taagtaaatt tccatatact tcgcaatttt
      721 tttcaatggt gaccctccaa tcaaaaacat atcttcatct atactttaac ataaataaac
      781 cgataaaatc cacatcaaaa ttatacacac aataaaaaag atatatatat ttaaacaaaa
      841 ctatttactt taagttgtaa gcggtgttat cattaagtgg aaacaggagg taaaaaaatt
      901 atgacaacta tgaaaaagct aacttttcca gatgattttt ggtggggttc cgcttggtca
      961 gctgagcaag cagaaggaaa aggagaaaca ggaaaagcag agactatctg ggatcgctgg
     1021 tttaaagaac aaccttaccg gttttatcaa cgaatcgatt cctctattac aaccgatcac
     1081 atccatcaat ataaagaaga tatagaacta atgaaaaaaa ctgggcataa ctcctttcgc
     1141 gtttctattt cttgggcacg aatgtttcca aatggcggta ccggaaaagt aaatcccaaa
     1201 gccatttctt tctatcgaaa attattttcc gaaatgaata ataacggaat caaagtattt
     1261 gccaacttat atcattttga catgcctgct actcttcaag ataaaggagg atgggaatca
     1321 cgtgaagtag ttgatgctta tgttcattac gctgaaactt gttttaaaga atttggtgac
     1381 ttagtttgtc attggtttac cttcaatgaa ccacttgggc ctattttagg tacttatctt
     1441 gaagattttc attatccaaa cctcattgac tttaaacgcg gagcgcaagc agcattcttc
     1501 actatacttg cacacgcaaa agcaatacaa gtatttaaag aactagctct tcctagtaaa
     1561 ataggcgtta ttctaaattt gagtcctact tttcctcgta gtcaaaatcc tgctgatgtg
     1621 aaagcagcag aaatcgctga tttattttat acgcggagct ttttagatcc tatggttaaa
     1681 gggaattttt ctaaaaagct tatcctgtta ttacaagaat ataatcagct tcctgaagat
     1741 tatacgaaag aagatcttgt atgtatctac gaaaataccg ctcaaattct tggactaaac
     1801 tattacgagc cacgtcgcgt caaatctcgt tcttctatgg tggatacatc tagtccattt
     1861 ttaccagagt ggtttttcga accacatatc atgcctggac gacgaatgaa cgaataccgg
     1921 ggatgggaaa tttacgaaaa aggaatttat gatttgtgca tggatatcaa gaaccattat
     1981 ggaaacatcg aagcatttat ctcggagaac ggaatgggcg ttgcggatga agaacgtttc
     2041 atggatgata cagggcaaat aattgatgat tatcggattg agtatatcaa agatcatctt
     2101 gcttacttat ggaaggcaat aaatgaagga tgtaacatta aggggtatca tctttggaca
     2161 tttattgatt gctggtcatg gatcaacgcc tataaaaacc gatatgggct ggtttcactc
     2221 gaccttacta ctcaaaaaag aacaattaaa aaaagtggcg aattttacaa gcaattaagt
     2281 gatacaaatg gatttagata cgatgtagac ttgttagttg aataatattc taaaattcca
     2341 cttgaagtga acacattaga aaacaagaat aatgagaaat accaataaca aatacttttt
     2401 cttcatactt catttatact tgtcagtata agagactgtg acaaatgtag tgatcctcag
     2461 aagttaggtc tggaaatcta acttgagaag atttttttaa actacgtcca gtctttttta
     2521 tttgtttaaa attgaagttg acaagtcttc attaaaaatt tagaatattc acaagtttga
     2581 cgacaattat gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga
     2641 tataaaaact gcgatcaaag acacgttacc taccgttttt ggttatatcg gtattggact
     2701 tgcatttggt atcgttggga aagctgccgg gtttcatccg ctggtcgtca cgttgatgtc
     2761 cctgctgatc tatgctggtt ctgcccaatt tatcacggtc agcatgcttg ctagccatag
     2821 tccactgctc tccatcgttc tctcgacctt tctagtcaat tcccgaatga ttctgatgag
     2881 tatgacgatt gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac
     2941 attgctgaca gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa
     3001 gttaaacttt cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc
     3061 ttctcttgtt ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga
     3121 tttcgccatc gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa
     3181 cacctctaaa cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg
     3241 actgattttt atcccaagta acttagtgat tttagttgta acactgattg ggtgcggatt
     3301 gggggtgtgg atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta
     3361 ctttagcaac ttggctttct aggatcctac catttgtctt actgaaaaaa ttcgatttgc
     3421 cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt
     3481 tcagcagtct atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt
     3541 tggcttcagt tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct
     3601 tggtaggaat tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta
     3661 tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg
     3721 tattcttgct tatccgttga acggaccact aacaacgact tttcctaata tatgtcctgt
     3781 ttctacgtct ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt
     3841 gctcaatgta ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc
     3901 tccttgcgtt tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa
     3961 gctgatagat tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt
     4021 tcccacatgt ccaaatggtc ggacaaggtg tttgatttga tcaaagtagc gggtaatatc
     4081 gaataataca gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa
     4141 gtcgtgatga taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt
     4201 cggacttgca gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga
     4261 accgacgccc ccagctccgt tgatcactaa gatttttttc ccgcggttgg cattttcttc
     4321 tggagtcaga ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa
     4381 ggctgcggct tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac
     4441 cagttggtat tcttgattgc ttcctgctct tgtcgttgta cctgcgtaaa aaacgcgatc
     4501 gccgacagaa aatttttgca cttcatcacc tacagcaaca actttcccga ctccatcaaa
     4561 ccctaagata cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg
     4621 attaacagat actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg
     4681 ttctttttca tcaataaagc tttttggatc atcgatcgac aacccttcat aaaaaccgac
     4741 taccttcatt aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc
     4801 atccatacag ttttcatttc aatatatata gtatgctatt tgttccactt ttattcatca
     4861 taattattct cttacttaaa gtaaactatt tgcacttttt tggttaaaaa agaggttgcg
     4921 acaagacttt tgtcacaacc tcttttaaac ggtgttcaat caactgaccc tcggtattcg
     4981 ctcaaaaaac gacaaaatat gaggagctat ttttgtttgg ctgttcttat tacttgatgc
     5041 aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca ttgttcactg
     5101 aaaagcgttg gatgttcttt ttcaaaggtg tctaagatct ctcgtacttc atcagtagat
     5161 ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt gatatagatt
     5221 ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata atgatcttgc
     5281 agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc cctcggttct
     5341 ttttcaaatg cataaggatt cttgaaaatc ccccggccaa tcatgatgcc atctacgcca
     5401 tatttttcgg ccaattcttc tcctgtctgg cgatctggga tatctccgtt gatcgtgatc
     5461 agtgtttgcg gagcgatttg gtcacgtaaa gcaatgattt tagggatgac ttcccaatgt
     5521 gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt ggctatatct
     5581 tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata gccgatacgc
     5641 gtcttgacgc tgacaggcaa accgccagct ttagccgctt caatcaattg tgccgctact
     5701 tctgggcgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg gactgggcag
     5761 cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct catttctcta
     5821 aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaag
     5881 gtcaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc tgagtttgta
     5941 aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg aaatacaaca
     6001 tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa
     6061 ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc aatctttctc
     6121 aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag cttaaaagat
     6181 gtttctgatt gagcaaaaaa agactgtgac aaaaatctta tcacaattcc ttttccggaa
     6241 taaatggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaaacga aaaataggag
     6301 gagctatttt tgtttggctg ttcttattac ttgatgcaga acagcttttt cacaacctct
     6361 ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaacaaacg acaaaatatg
     6421 aggaactatt ttcccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct
     6481 cttttctaac ttctcaatcc tcttccttta ttgatcaaat accaagtaac ggcgtacaaa
     6541 acaacgatga atagcacaag tacgatcatc gatgttgcta ctggcacatc gattgttcca
     6601 aggaatccgt aacggaaacc ggagatcata taaacgatcg gattgacttt cgaaacagct
     6661 tgccaaaatg gcggcaacat agagatggca tagaaaacgc cacctagata agttaacggc
     6721 tgcaagacaa atgtaggcac gatcgataca tcgtcataag actgagcaaa aatcccgttg
     6781 agcagtcctg ctaacgaaaa caagattgcc gtcatcaata atgtaatgat gaccatgaac
     6841 catgaataca catgaagtgg tacaaagaac aaggaaatga tcgttacgag agagccgacg
     6901 agaatactgc gacccactcc gccaatcaca aagccccaaa tgatcacatg cgtaggaaca
     6961 ggcgctacca agatttcttc gatgtttttc tggaattttt gagagaagaa ggacgaagaa
     7021 acgttggcat acgaactagt gatagcagac atcataatca aaccaggcac gatgaattcc
     7081 atatatgaaa aaccacccat gtcaccgatc cgaccaccga tcatttttcc gaaaataaca
     7141 aagtacaagg atgtcgtgat cacaggcgga accagtgttt ggacccagat acgcaaatag
     7201 cggttcgttt ccttgatagc taaacttttt aatgcagtaa aataaagatt aaacattttt
     7261 ctcctccgta tgattatttt cttctgtgat tttcaaaaac agttcttcta aacgattcga
     7321 cttattcctc atagaaagaa ctttgattcc ttgtcttgtc agctgatcga agattccgtt
     7381 caccccttga tttcgttcaa cttcgacagc gattgtctgt tcatcttcaa atgtacaagc
     7441 gtatccttca atcacaggtt tggtctcata tggcgctaaa tcaaagataa atgtttcaaa
     7501 ttgcagtttg gatagaagct ctttcatact agtattctcg atcagttcac ctgattggat
     7561 gatcccgatg ttgcgacaaa gcatttcagc ttcttctaaa taatgcgtcg tcaaaatgat
     7621 cgttgtacca ttttcgttca attctcggag aaactcccac atctcgcgtc tcaattcaat
     7681 atcgactcct gctgttggtt catccaagat cagcaaacgt ggttcatgca tcaatgccct
     7741 agcaatcatc agtcttcgct tcattccccc ggaaagcata cgtgcacgga catttctctt
     7801 ttcccaaaga ttggactgct tcaaatattt ttcgctgcgt ttcatcgctt ctttgcgaga
     7861 gacaccgtag tagcctgctt gattcacgac gatttgctgg accgtttcaa agggattgaa
     7921 attgaactct tgcgggacta atccaatctg ttgctttgct cggactagat ccgtatccag
     7981 atcataaccg aagacattca cttttccaga tgttttattg actaaagaag taatgattcc
     8041 gatcgttgta gacttcccag ctccatttgg tcctaatagc gcatagaaat ctccttcttt
     8101 cacggaaaga tcgattcctc gcaatgcttc gaccccactt ccataaacct ttttcaaatt
     8161 atttatttca agcgcatttg ccatttattt cctatctcct tatagacatg attctttatt
     8221 gtatcatgga atacagcaga tgaaaaaaca acttggttcc agcgaaaaat caggttgctg
     8281 ggctcaggaa aatctcctaa tgaaagacat caatcggacg aagtcagaca accttttcag
     8341 aaaaaacaaa tcgaagataa aggattacta ttcatatgtg caacaaaagg agcgtgagac
     8401 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcaaaagc
     8461 caagattttt gatccaataa atactttcga tcacttcgcc tatcccaata catgccactg
     8521 ccgcttttca tcaaataacg cggcttccgt ttttgcggtt tctactaaag tctctgggta
     8581 atccaaaact ttcagcaagg caatcgcatt tcgggtcctt gaaggtcctt cttttaattt
     8641 gaaatcaaaa ctgattcctt ttccttctgt tacttgttct tcaaagtgga cattctcaca
     8701 ataattcttt aaaatctctg tcaattcgat atcatgtgtg gcgacaaatg ctaaagatgg
     8761 atactccgca agccaccgaa cgactgaagc ggaagaagca atccgttcga tcgtatttgt
     8821 cccttttaaa atctcatcta cgaagcagta gcagcgttct ttggaagcca cttggtccaa
     8881 caaacgtttg atcgatttga tttctgaaac aaaataactg tcgccttcaa ataaatcatc
     8941 ttcgattgcc atggaagtca acacatgtcc aggttgcagg gtaaactgtt ctgcaatcgc
     9001 tgtttggatc gtctgtgcta gaatacagct gatggctata cttttgacat acgtagattt
     9061 tcctgatgca ttggaacccg tgactaatgt attcttctgc cagttgaccg gattcactac
     9121 cgcttctttt aacaatggat gatagatatc tttcgctgtc acaccgccct cagtaaactc
     9181 tggtcggcaa gtgatgggca tataagtacg gtaattcaat attgcagcgg ctacttctaa
     9241 ttctcccagc aattcccaca gcttgatggc ttcagttgaa tgcttttcta atttagtgag
     9301 aacgaaatga taggagataa atggcagcat gaacatgatg ttgacatatt caaaaatcaa
     9361 ctctgcttca ctcccactct tcatacgaaa tgaaaaacct aatttaggga tgtctcttaa
     9421 tggtttagtt tgctgtttca gctcttcttg aagtggtgtc ggcagttttg ctagctgatt
     9481 ggcggctgaa atcgttgaaa ccaaataact catgctattc agctctgttt ctagtgtttg
     9541 tttcttcact agataataga ccgtattgaa tactaaccca ccgattgcca aatagattcc
     9601 tagtacttgt cctaaaagaa gcaaacccaa acccaccagc ggaagcaagc caagaaaaat
     9661 aaataaacct acgcgtccaa tctttttact ttttccatcc gctagatatt gttttgcaaa
     9721 attatgatct ttttttccta aacgagcgaa ttgatattgg attttttctc tcagttgagg
     9781 attttcctga taaaaagcaa tcagtttttc taactgctga tcttcaccaa aatcaaagct
     9841 gcgtaaacgt tgatacaacg cttctgaacc tacactagaa taggtactgt tgattccttc
     9901 gaatacttcg aacatatcca ggtcatacca agtaagatca tcgatttcgc tatcccatga
     9961 acggaacttc ttctccgtga gccatgcttc cttcaagctt tcttctttat caaatctcgg
    10021 ttgataagga attttttccc atttgctacg aactgtttcc tttaatttca aacgtgaaaa
    10081 aatgtctaaa gcaactaata aaataacagc tgcaataata attaaaacga taaattctct
    10141 ttgacccata tccctctccg ttcctttatt tgttagcttt agtatagcac tgttgcgcta
    10201 tttgaaaaga gggtaaaaaa acacctttca aaataaaagg tgtttttttc ttgtatatta
    10261 catatttatc tctcatttat caaaattgac aaataatcat agggaggaat tgttgttaat
    10321 tcttgtttat tctccagtgc attgacaact gtcccaaggg aaaggatagc ctcaggtact
    10381 attttttctt gttctgttgg attagctaag cgaagaacaa atccttgttc atgaaaataa
    10441 gaaggataaa tcgatgatac aattaagtct tcaggtaatt caatcaatga aaatttacga
    10501 ggaacatcaa gttcattttg taatggccaa atcttgttat ctaacctgtg aataaataca
    10561 ttcagtttct gcttttgata agaaatagat tgttctaacc gtttttctgc ctgttttgcc
    10621 actttgaact cattaaatct tccttcttct acattaaatc caaaagtgac tttatactca
    10681 cctatctctt gagctagcgg tgtttccatc atgacatgcc cttcatttgt cgtatctcct
    10741 gaagctcgcc caggtcgcca agcaagatta ggttttccaa gttcccctgt tgttgaaaat
    10801 aaggttatga ttaattgatt ttctatacgt tcatactctt ttaaaccatc tgcataaaac
    10861 gtaaaataat gatcattttc tgccacagag acactcttat cgaatatttc taaattaaca
    10921 ggtttctcta ccatagtttc tgaccaattc ggaggaatga catcaggggt attttcaata
    10981 aatccgtttt gaatttgtgc aattgatttt tcagatttat ctaaaacatt aattgctaag
    11041 cgcatacgat gcgagtaaac agtattatga attttgagtg tgccttcaac gatttgactt
    11101 tctcccagac tgaacgatat ttcataagat atttcttcca tttcgggttt ttcagataaa
    11161 cgatctttta aatctttagg aagttgagct tttccgtaaa caactaatgt ttcttgtaaa
    11221 gaatctttat atactttagc tgtttctaat tttagagata gttctgtatc tccttctaat
    11281 ggtgaataat catacgtatc gccatcattt gctgaatcga tcaaatgaac gaaatcaaga
    11341 tattgtctat ctccaactat caaattcaat tgcccatctt taaaagaaag tttatattta
    11401 ttgttactta tctcagtttt gtctatctcg attgctgttt ctaatttttc attatttttc
    11461 tcaaattcaa caacagtgta tcctagtgct ggcacattta catttatttt tatgtcaagt
    11521 tcataataag ctggttcatc aaaatattcg aaacctgtag caatcatccg ataagcattt
    11581 ttccgttctg gaaaataaac ttctttttca atgatattat agggactacc tttgaagtgt
    11641 attttctttg aatcagctac tacatgcacg attttcgatc cactataatt ctcagtcgtt
    11701 gtgttgaaaa caaggacttc attcgatttt agctttaatg catcagccat ccgttttaca
    11761 ataaggtttt cgattccgtc agctatctca ttagcttctt tgattcgatg gaaaatatct
    11821 tccgcaacat tatctgaaac acagccacca atactatcat gagcctggtt ttgcaaaatc
    11881 tttttccata accgacgaag taggccttta ctgatatgga taccatttct ttctgcaatc
    11941 agcatgagcg gctcaactcg ttttagaatt ttttgttcca atttaaaatt ttctattttc
    12001 atttttgttc ttactgagcc aatagaacga tgaacacgag agtaaaccgg ctgacgaagt
    12061 tcacctttat attcaggtaa ttcttttttg tttctgataa cttctacata agaaggataa
    12121 tctgaaataa tattgtggaa tttactgtat ttattcaact tctctacgac ttgatcaaaa
    12181 tttaaaatca ttgacttttg atctacaccc gaaggtacta aaatatcttt gttttttcct
    12241 tgcatatgaa gcatttcaac tgcttcatcc aacttttctt ttgcaaattc aggaatatct
    12301 ttaattgcgt ctaaggtcat cattccagtc atataaccaa atggaaaatt catggcataa
    12361 acaaatttat ctcccaatcc tttccagata aaataaggag atggaaccat cgtttcaaag
    12421 tttaatcctc gccaacacat gaaattatcg atttctactt gatgcaaaag tgtaggcaaa
    12481 ttcgcattga acccaaacgt gtcaggcaaa taccctacca tcattggatc tccatattta
    12541 aggatcgtat cactgattcc aatcattaag tttcttaaaa ttgattcagc atccactaat
    12601 aaggcatctg tttgtgcata ccacggtccc acgaatagtt gtcctgtttt catcagtcgt
    12661 tggataacct taagcttttg cggattgatc tccaaatact catcgacgat tgaagtttgt
    12721 ccatctaaac aaaagtttac tttagggttc ctttccaatt catctagaac ttcagtaaag
    12781 acctgatcag acaatacgat ggcatcttca tttgtaaaat accattctct gtcccagtgc
    12841 gtgtgttgaa ttatatgtgc atttaccata tcagattctc ctactttcct tcttctactg
    12901 aatttacttg ttgttctttt ttatacttga tggattttag tgcattgacc attaacgctg
    12961 taaccactcc cccaataaca attgatacta aatataaagg tatattcgta acaaaaggaa
    13021 ttgccataaa ggttgaaagc attgtttctt gagtaacacc aaaaaatcca tttagaccac
    13081 atgctactgc agtgcccacc acgatgcttg gtattactcg taaaggatct ttcacggcaa
    13141 acgggatcgt cccttcagta atccccacaa ggcccataat catagctgtc tttgcattat
    13201 ctcgttccga ttcatcataa agttttttgt ttaaaattgt tgctaaaaac attccaaatg
    13261 gcggaatagc aattcctaat aacatggctg tactaggtcc aaagcttccg cttgaaaaag
    13321 aagcaacccc aaaagcatat gcgattttat tgataggtcc tcccatatca aacgccatca
    13381 ttcctcctaa aatagccgac atcaaagcag catttgctcc tgacatgcct tctagccaat
    13441 tagtcagtcc tgttgtcatt gcttgaatag gcgtagctag tacataccat aatatgattc
    13501 caacaactaa tgtagtaaca aaaggaatca gcataattga ttttagacta ataacttgca
    13561 tcggtacttt tacttttttc aatgcattag ctaaatagcc acaagctaat ccagcaacca
    13621 ttcctccaat aaatcccata ccaagatttt gacacatcat cccagtgata aaagctggtg
    13681 ctagaccggg acggtcagca attgaatatg cgatataggc tgaaatcatc ggtacgataa
    13741 actgtaatcc cgtctgacca atcattcgca ggactcccca gaatgtatcc gtctggtccc
    13801 aaacacctgt gccaccaaaa atgactgcaa tagaagttag taaccctgct gctacgacaa
    13861 gcgggatcat ataggaaacc ccactttgga aatgatttag aatttctctt ccttgtttct
    13921 tgaattccat tagtaactcc cccacgatat attttttatt tgttaattaa tgcaattgct
    13981 tcatcaatcg tctcttcacc atcttttact acctctgagg tccctactgt taaaatcggt
    14041 ttaccgtcaa agcgacctcg ttctgaaaca tttgtatcta ctgcaaatat gacaacatct
    14101 gcctcggaaa catccgaaat tgaaagtttg ttttctatcc ccatagcccc ctgtgtttct
    14161 actttaatat ctaacccttt cttcttcgca aacttttcta aattagcttt tgccatataa
    14221 gtatgggcaa tccctgcgat acatgctgtt actgctacta tttttgtcat atgaaattcc
    14281 tccttttaaa atgatagagc ttttaccaat tcttttttat ttctcgaatc ttttattctt
    14341 ttaacaaact cgtcatccat caatcttcca gataattctg ctaaaatacg taaatgatta
    14401 gtacctttat cagtgtcttt tattgctaac aaaaagacga gatcaactgg ttgatcatct
    14461 aatgcgcccc attcaacaga ccgctttaac ttggctacag ccactgttga ttcaattact
    14521 gctaggcttt tgccatgagg aatggcgata tttttcccaa ttcctgtggt catatggttt
    14581 tctctgtcaa atacgtcttt ttgaaattgt ttgtggtcag acacaaaccc attagtctct
    14641 aacaatttcg ataatttttc gactacactt tttttatcag atgcattt
//
LOCUS       DAPCMT010000063        14541 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPCMT010000063 DAPCMT010000000
VERSION     DAPCMT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.063"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..296
                     /locus_tag="RD743_001604"
     CDS             <1..296
                     /locus_tag="RD743_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDU0563419.1"
                     /translation="MFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVS
                     DKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            496..810
                     /locus_tag="RD743_001605"
     CDS             496..810
                     /locus_tag="RD743_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDU0563420.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            831..1208
                     /locus_tag="RD743_001606"
     CDS             831..1208
                     /locus_tag="RD743_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDU0563421.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1218..1991
                     /locus_tag="RD743_001607"
     CDS             1218..1991
                     /locus_tag="RD743_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563422.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2013..3416
                     /locus_tag="RD743_001608"
     CDS             2013..3416
                     /locus_tag="RD743_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDU0563423.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3598..4782
                     /locus_tag="RD743_001609"
     CDS             3598..4782
                     /locus_tag="RD743_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDU0563424.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4779..5069
                     /locus_tag="RD743_001610"
     CDS             4779..5069
                     /locus_tag="RD743_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563425.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5066..5287
                     /locus_tag="RD743_001611"
     CDS             5066..5287
                     /locus_tag="RD743_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563426.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5329..6108
                     /locus_tag="RD743_001612"
     CDS             5329..6108
                     /locus_tag="RD743_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDU0563427.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6175..6669
                     /locus_tag="RD743_001613"
     CDS             6175..6669
                     /locus_tag="RD743_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDU0563428.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6725..7363
                     /locus_tag="RD743_001614"
     CDS             6725..7363
                     /locus_tag="RD743_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563429.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7435..7830
                     /locus_tag="RD743_001615"
     CDS             7435..7830
                     /locus_tag="RD743_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0563430.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7814..10267
                     /locus_tag="RD743_001616"
     CDS             7814..10267
                     /locus_tag="RD743_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0563431.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10264..12291
                     /locus_tag="RD743_001617"
     CDS             10264..12291
                     /locus_tag="RD743_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563432.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12288..13310
                     /locus_tag="RD743_001618"
     CDS             12288..13310
                     /locus_tag="RD743_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDU0563433.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13327..14256
                     /locus_tag="RD743_001619"
     CDS             13327..14256
                     /locus_tag="RD743_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0563434.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         4927 a         2444 c         2887 g         4283 t
ORIGIN      
        1 agatgtttaa atctcgagat ggtatcacac ttactacagc acataaagtt aaaggaaatg
       61 agtatgatac tgttatcgct attaatcttc taaacgggaa aattcctcat tggaatgaaa
      121 tttttaatgt ttctgataaa ggaattagtt cagcgagaaa actactatac gtaatttgtt
      181 caagagcaaa aaataattta tttcttttct cagaggtggg ttattttacg agtaaaggag
      241 acttgcttta tcccactcaa ttattagata aaatagaatt tgattataat atttaaaata
      301 cctaggtagt cgttgtttta aatatagtag gaatcggacg ctcagtacgg agcgtctatt
      361 tttttaccca attttgaaag gaagtgaact tatgaaaata aaaaatcaag aatcaaaagg
      421 tcgctctccc ccctttaaaa ccatcacaca aataagattc attcaaacat aaaaacagaa
      481 aggataggta aaaatatgga acttaaatat gtcattccca atatggaaaa aacatttgga
      541 caattagaat atgctggtga agggaacatt gaacaacgtc gaataaatgg cagaccaacc
      601 attctctctc gtagttacaa tttatactca acgattcaac gagcagatga tattgttgtc
      661 attcttccct ccgaagctgg cgagaaacat tttgaatccg acgaaaaagt caaactcatc
      721 aacccacata ttaccgcaga aggttataaa attggcaatc gaggatttac caattacatt
      781 atgcacgcag acgatatggt gaaagcataa gaaaggaaag gtgaaaaaat atgagattag
      841 cagaaggtat tgtgattgaa aaagaccaga catttggcac attgaaattt tccgccttgc
      901 gtcgtgaggt tcgtatccag aatgaagatg gtacggtttc cgaagaaatt aaagaacgta
      961 cctatgactt aaaatcaaaa ggacaaggac gcatgataca agtcagtatc cccgcttctg
     1021 ttcctttgaa agaatttgac tacaacgcag aagtggaatt aatcaatcct gtggcagata
     1081 ccgtagcgac ggctactttt cgaggggcag acgttgattg gtacgtgaaa gcagacgatc
     1141 ttgtattaaa aggacagaca aaacctcatc agaaatataa aaaagaagcg tccgaaagtg
     1201 aaaaataggt gttgcttatg acaaacacac cattgatttt gaaagaaatt ccgaaggatg
     1261 aagccatttc ttttattcgg caataccatt attcaaagat actccctaga ttgtgtaaat
     1321 actttctagg gattttttca gaagaaaaat tattaggcgt tgtggaatta ggctggggta
     1381 cgcaacccct acaaaccata cgcaaactct ttcccgacag ttcccttcaa accacagatt
     1441 atttagaaat tggaaagatg tgttttcttc cagaaatgaa tcaaaccaat tattttggca
     1501 gtcaagctct ttcagctcta attaagtggt tgaaagaaca tacggactgt cattttttat
     1561 atacactggc agatggaatt gagggaaaat gtggctatgt ctatcaagcg tccaacttct
     1621 tttactgcgg gtatttcaag acaagtgtct atcgtgataa gcagtcatgg gaaaagatac
     1681 accctcgaag tgctcgcctt ttattagagg aaaatgctcg ctttgaacag gtagagaaaa
     1741 agcattggct ttcccaggca ttttgcgaat acaaagggat tgagaaaatc aatggacgca
     1801 tgtttcgcta tctctatccg ttgacaaaag aagcgaaaaa actgttagga cacactctct
     1861 atcgacgtca ttattatccg aaagaaaaaa atttacgctt tgaaaagcga atcgcttatc
     1921 agaaatatga agccatttct caaccaactt ttgataaaca ggctcgtatc tacaatacac
     1981 aattattttg ataggtggaa aggaggattt ttatgccatt gcttcatgac agaggagagc
     2041 gaatcaaagc caacgataag cacttggtct atcattttgt actaatttca ttgctggtag
     2101 tttttctagt cgtcctactc ccttttcatt ggagagcgtt acaatcggtg aactggcaag
     2161 cagttcagtt agatacattt ttctatcaac ttcatacgcc atacttttgg agcagtattc
     2221 tcactgctct ttttgtatgc ggacttatca gctggcttta ttatcactat cgtatagatt
     2281 ctataaaaca gttagagcac cgtcaaaagt tagctcgtat gattttagaa aatcattggt
     2341 atgaagccga agaaactcaa tcagagggat tctttaaaga tttacccagc aagaaaacca
     2401 aacaacgaat ccgccatttt cctaaaatat attatcgaat gaaagatgga ctaatccata
     2461 ttcatgtaga aatcacctta gggaaatatc aagatcagct tttgcaccta gaaaacaaac
     2521 tagaaacagg attgtattgt gaattagtgg aaaaaacgtt gaaagattct tatgtagagt
     2581 atgtcctact ttatgatact attggcaatc gaattggcat tgaagacgtg gtagctcaag
     2641 atggacgtgt caaattgatg gaatcggtct attgggagtt cgattctctc cctcacatgt
     2701 taattgctgg gggcactggt agtggaaaat cctattttat tctcacattg attgaatctc
     2761 tcctgcatac aaatgcaaag ctttatattc ttgaccctaa aaatgctgat ttggcagact
     2821 taggggcggt catgaataat gtctattacc gtaaagaaga tatgttggca tgtatcaatc
     2881 aattttacga agatatgatt gatcgcagtg aaaccatgaa acaacaccct aactataaaa
     2941 caggagaaaa ttatgcttat cttggtttgt ctgccaattt tcttattttt gatgaatatg
     3001 tggcaatgat ggatatgcta ggtcgtgaaa gtaccaccgt tattcataaa ctcaaacaaa
     3061 ttgtaatgtt aggacgacaa gcaggcttct ttttaattct tgcctgtcaa cgtccagacg
     3121 caaaatattt tagtgatggg attcgggaca actttaactt ccgtgtggct ttgggtcgta
     3181 tgtctgaaat gggctttggt atgatgttcg gaagcgatac acaaaaacaa tttttcttaa
     3241 aacccatcaa gggaagaggt tacgtggata caggaaaaag tgtgattagt gagttttata
     3301 caccccttgt accaaaaagg tacgatttct taggagaaat aggaaaagtc attcaaaaaa
     3361 agcaatctga gcctgtaccg cacgaagtga aaggtacagg ttcagacttg ctttgattgg
     3421 tgtggcgtta gccacgccaa tcctttagcc ccctctatct aacagggggg cacaaataca
     3481 actgaaaaca ggcaattctc atcaaccaat gttgatggta caaaggattg cggaaaatat
     3541 gaaaggtgta aactcaaggg tcatagttta catctttttt agattggagg tttgcgaatg
     3601 gaaaacgaaa aaatttggat taaggaattg aaagaaaagc gactggctta tggcgtttct
     3661 caaaataaac tggcggttgc ttctcatatt accagacctt acctatcgga tattgaaaca
     3721 ggcaaggcag tccctactca acaggtgaaa gaagatttac tgaacgcttt ggaacgcttc
     3781 aatcccgata acccattgga gatgttattc gactatgtac ggattcgatt ccctaccaat
     3841 gatgtggctc aaattatcgg tgaggtttta cgtttgaata tggattatat gctacacgaa
     3901 gactttggct attattctta tcccgaacac tatcgtttcg gtgatattgt tgtcctagtc
     3961 tctcatgatg taagtaaagg tgttttatta gaactgaaag gaaaaggctg tcggcaattt
     4021 gaaaactttc tattggctca acatcggagc tggtatgatt tctttcaaga ttgcatggaa
     4081 cataaaggta ttttcaaacg cttggattta gctatcaacg ataaaacagg gattttgaat
     4141 attccagatt tggcaaagaa atgtaaacag gaagaatgca tttccgtctt tcgaagcttt
     4201 aaaaattatc gcagtggtga attggttcac cgtgatgaaa aaccatacat gggaaacaca
     4261 ctttatattg gctcattaaa aagtgaagtc tatttctgta tatatgagaa agattatgag
     4321 caatacgtga aaaatgatgt tcccttagaa gacgcagaag taaagaatcg gtttgaaatt
     4381 cggttaaaaa atgaccgagc cacccaagcc atgaaagatt tattggcaca tcaacaggcg
     4441 gaaaaaacag cctttgaaat tatcaatcgc tatatccgct ttgcagataa agacgacact
     4501 aaacgtcgga gtgattggaa aactaatgaa cgttgggaat ggtttatcgg taaaaatcgt
     4561 ggagcattac gcctaaccac acagccagag ccttattctt ttgaacgaac attgaactgg
     4621 ttacatcatc aagtcgcccc tacattaaaa atcgcaagca ttttagatgt tctcaacggt
     4681 acaaccatta tctccaccat gattcaggaa gccaaactga cagaaaaaca tgagaaatta
     4741 attgaacaac aacatctagc catggaggat ttgattacat gagcctttta acaaaagaat
     4801 taaagaagtt agggtttcag tgtggcattg agtttcaagc gtatatacaa aatacaggga
     4861 aatatacgtc tctcattatt gagggaaaac ggcaagctgg tgataccatc tatacctatg
     4921 atttttacaa ggtgagattt tataataatt ataccaatcg agtaaccgtc tatggagagc
     4981 atttaacccc ttttcaatta ttaaggcgtg taaaaagcta tatctattat cgagaaaaat
     5041 atcttaagga aaggaggaca atcacatgaa ttttgggcag aatttgtaca actggttctt
     5101 ggataatgca cagtcattgg tattgttagc gattgttgtc attggactgt ttttaggttt
     5161 taaacgagaa ttttcaaaac tcattggctt cttgattatt gctctaatcg ccgtcggact
     5221 tgtgttcaat gcagctggcg tgaaagatgt gttattaaac ttatttaacc gaatcattgg
     5281 tgcttaacag atatttttca tgataaaata ggaagtatat attttgttat gaaaggattt
     5341 atgaatatgt ttacaaatga tcaatttaaa tctcttttaa ttaaaacatt agaaagaaag
     5401 agctcttcaa attattatga aggaggtaac gagattgttt ctaaaatgaa aatatcagag
     5461 gtaactttag atgagactga cgattttact tattataaag gatataaaaa gggctggaat
     5521 acgctttgca catatcttaa aataagagtt ccttttgatg atttagactt ttttgaaagt
     5581 cataaagatt tgattaccca gacagcaagc tccatatatg ataaacaagg tgataatgta
     5641 ttagtggata ctattttagt accacttcca gagaattacg aagtaattaa ctttagtcag
     5701 ttgaaaatta gtgatgtggt ttcacaagct attgaagacg ctgagtcttt tatgagtaat
     5761 ggtgaatatc aacgtgcctt tgacagagtt cacactgctt ttcatggata tttaattgaa
     5821 atactaaaaa aatatgaaat tacagttcct agagacgaaa atctatctaa attatatagt
     5881 aggatacaac aattaattga aaaagaaatt caacctactg aacttgctga tatagtgaag
     5941 acaacgataa ggtcatcaaa tggaatgatt agctctctta atgaagcaag aaataggcat
     6001 tcacttgcac accctaatac aaatattatt ggaaagagag aagcaaaact aataataggt
     6061 atttcaagta cagttacaga ttatattagc ggataccttg ataagtagcg tcattaactg
     6121 attgctactt tttttatgac tatttttata aaaaggatgt gataagaatt ggaaatgaaa
     6181 gtctatgtgg caaatttagg acggtataat gaaggtgaat tagtcggagc atggtttact
     6241 ccaccaattg atgaggaaga aatggcagaa agaatcggtt taaatgagga ctacgaagaa
     6301 tatgcaatac atgattttga gcttcctttt gatgtggacg aatacacgcc gattagtgaa
     6361 atcaaccgtc tatgtgaagc cattcaagaa atcgaaggta cacctattta taatgaactc
     6421 aaagaaattc aaggcatgtg gtttagtagc ttggaagaat tactggaaaa caaagaggat
     6481 attcattgtt atagtgattg tgattcgatg gaagatgtcg cccgttatta cgtggaagaa
     6541 acaggacagc ttggtgaagt cccttccaat ttacaaaatt atattgacta ccaatctttg
     6601 ggacgtgata tggaaataga gggtaattac ctagttacct ctcatggggt atttgaatat
     6661 tgtcagtaag tttaacaatc cgtgttataa ttaaattaat aaatagcacg gagaggtata
     6721 aattatggta aatgtatttg aaaatcttaa actattaaaa ctagacatga aagataaagg
     6781 ctgggtgata gattcctttt actttcgtta caaacagcaa aattatattg tacttgttaa
     6841 gctatttgaa aaagaggaaa aagttccaga atatgctctg ttaaaactag aatttttaaa
     6901 ggaaaacgat ttttcagata tgctagctgt ctatgctaat tcggtaaaat tatatacaga
     6961 cacgaaaaca ataagagaat attttggtat cgaatacagt agtaacttgg gagatgtttt
     7021 atttcaattt agccaaactc ttgctcgatt tattccgaca gaagttagtg agaagaaaaa
     7081 tgaagaccaa aaagaagcca tgtgtttctc attaagtcaa agtgattcag aagatcccag
     7141 aaaaaaatat tgtttttcag ttagaagaaa tcctttaaag ggaaatggtg agctgggaaa
     7201 acgaagtcct tttaatgaca ataaaacgag attgtataga cccagcttat atgaacgatt
     7261 agggtcagat accaatttaa gtttcagata ttcaatgaat ccagatgatg aagaaactga
     7321 tgaaggtatt attgcaaaat ggacaaaaaa taaaattgaa tagttaaagc attgaaagag
     7381 caatcaccta ttttgattgt tctttttcta tttttacgga aaggacgtga agacatgaaa
     7441 aaaataagaa gttacaccag tatttggtca gtagaaaaag tgatttatgc gattaatgat
     7501 ttacaactcc ctttcccaat tacctttacg caaatgacat ggtttatcgt ttctttgttt
     7561 gtcgtcatac tctttggcag tttaccacca ctttctctga ttgacggagc actactcaaa
     7621 tacttaggga ttcccattgt tctcactggg ttcatgtgcc aaaagacctt tgacggaaag
     7681 aaaccctatg gatttctaaa gtctatcatt acctatgctc tacgccctaa agtaacctac
     7741 gctggcaaac aaatacgatt aaaaaaagaa acaatgaatg aatcgattac gattgtaagg
     7801 agtgaacaac tagatggcat atccgattaa atatatggaa gataatctcg tatttaatca
     7861 tgatggcgaa gtattcgctt attatgaatt aattccctat aattacagtt ttttaagttc
     7921 cgatgaaaaa attcaagttc atgataattt ctgtcaattg attgcccaaa atcgtgatgg
     7981 caagattcat gctctgcaac tagcaaccga atcaagtatt cgagccacac aggaacaatc
     8041 gaaaaaagaa atcactggac gattaaaaga gatagcttac gaaaaaatag atgaacagac
     8101 cgaagcgtta atttctatga ttggtgacaa tcaagtagac tatcgctttt ttattggttt
     8161 taaactctac ctcaatgaac aggaagtatc cgtaaaaaat gttgggaaag aaattcaaaa
     8221 agcatttgcg gaattctttc atggtgtgaa tcacaaatta atgggcgatt ttgtttctat
     8281 gcctatggcg gaaatcgaac gcttttctaa gatggaacag ttgttagaaa gtaaattatc
     8341 cagacgcttt cagattcgta aattggagaa agatgatttt ggttatatta ttgaacatct
     8401 ttacgggcaa acaggtgtcc cttttgatga ctacgactac catttaccga aacgatacga
     8461 agataaaaaa gtgttggtaa agaaatacga tattctcaaa cccactcgct gtttaatcga
     8521 agaaaatcaa cgctatttac ggattgaaca ggaagatacc actacttatg tggcgtattt
     8581 tacaattaat agtattgttg gtgaattgga tttccctaat agtgaaattt tttattacca
     8641 gcaacagcag tttactttcc ccgttgatac cagtatgaat gttgaaattg tcgccaataa
     8701 aaaagcctta tctaccgttc gcaataaaaa gaaagaactg aaagatttag atagccacgc
     8761 atgggaaagc gacaacgaaa ccagtactaa tgtggtggac gctttagaat ctgtcgatga
     8821 attagaaagt aatttagacc aaaccaaaga atctatgtac aaactaagct atgtggtacg
     8881 ggtatcagca aagaatttgg acgagctgaa acaacgttgt gatgaagtaa aagattttta
     8941 tgatgatctg tctatcaaat tagtacgacc ttttggcgat atgctaggct tgcataatga
     9001 atttattccc tcaagcaagc gatatatcaa tgactatatt caatatgtca cgtcggattt
     9061 tttggctggt cttggtttcg gtgctacaca aatgattggt gaaaattctg gtatttacgt
     9121 cggttacaat ctcgatacag gcagaaatat ttatctccaa cctagccttg caagtcaagg
     9181 aattaaaggc tctgtaacaa atgcattagc gtccgctttt attggttcat taggtggcgg
     9241 aaaatcattc tctaacaata tgctggtcta ttattcagta ctctttggcg gacaggcaac
     9301 cattgttgac ccaaaagctg aacgtggtga atggaaagaa accttacctg atattgccca
     9361 tgaaatcaat attgttaatc tcacgagtga gaacgacaat aaagggttac ttgaccctta
     9421 tgtgatttta aaactcccga aagattcaga aagccttgcc attgatattc tcacgttctt
     9481 aacaggtatt tccagccgag atagcgagaa gtttcctgtc ctgcgtaaag ctattcggag
     9541 tgtaactcaa agcaaacaac gagggttgtt attggtgatt gatgaattgc gaaaagaaga
     9601 tacgcccatc agcaatagta ttgctgacca tatcgaaagc tttgtcgatt atgattttgc
     9661 ccatctgtta ttcagtgatg gcagtgtcac ccaatccatt agcttggaaa aacaactcaa
     9721 tattattcaa gtggcagatt tggtcttacc cgattccaat agctcttttg aagaatacac
     9781 taccatggaa ttattaagtg tggcaatgct gattgtcatt agtacctttg ctttagactt
     9841 tatccactct gaccgttcca tttttaaaat tgttgacctt gatgaggctt ggagtttttt
     9901 acaagtggca caaggaaaag ctctatctat gcgattggtt cgagcaggac gtgccatgaa
     9961 tgcaggggtc tatttcgtta ctcaaaacgc ggacgattta ctagatgaaa agatgaagaa
    10021 taatattggc ttgaaatttg cctttcgttc cactgaccta gtggaaatca aaaagacctt
    10081 agaatttttt ggtgtggaca aggaagatga aaacaatcag aaacgcttac gtgaattaga
    10141 aaatggacaa tgtttagttt cagacatcta tggtcgtgta ggggttatgc agtttcaccc
    10201 gatttttgaa gagttgcttc atgcctttga taccagaccg cctgtgcgaa atgaggtgga
    10261 agaatgacac aaaaacaaaa gaaatttctt cactatagtt ggataacgct cctttgtgtg
    10321 ggcgttattt tacttttatt aggcacgctg ggcaatgcag tacaagcgac aggcttagtt
    10381 gatgaaacca ttgatacaag caatgaatac tccaagtatg gtttgaatca atatcagctt
    10441 gattattatg tggacaatag ctggggctgg cttccatgga attggagtga tggcatagga
    10501 caatcggtga tgtatggatt gtatgccatt actaatttta tttggacaat ttctctttat
    10561 ctatcgaacg ccacaggtta tttggtgaaa gaagcctatg ccttagattt cattagtcaa
    10621 acatcccaag caattggcga aaacatgcag acgattgcag gcattacacc gaatggcttt
    10681 tccagttcag ggttttatgt cggtttcctc cttcttttta ttctcattgt tggaatctat
    10741 gttgcctata caggattggt taagcgagaa acgacgaaag tgatacgagc tgttttaaac
    10801 tttgtcgtgg tgtttattct atcggcttcc tttattgcgt atgcacccga ttatattgcg
    10861 aaaatcaatg atttttccaa agatgtcagt accgccagtc tgaatattgg gacaaaaatt
    10921 gttcttccta acacggatag tcaagggaaa gacagtgtgg acatgatacg tgacagctta
    10981 ttctctgttc aagtaaagca accatggcta ttgcttcaat atggctccac tgatgtagaa
    11041 gcgttagggg aagaacgtgt ggaaagttta ttgtctacca gtccagatac aaacaatggt
    11101 gaagatagag aaaatgtagt gattgaagaa attgaagatc atgacaatac ctatttaacc
    11161 ctaccgaaga cgatttccag attaggaaca gtcttctttt tgtttgtatt caatattggt
    11221 atctcgattt ttgttttcct tctaacagga atcatgattt tttcgcaagt gctatttatt
    11281 attttcgcta tgtttttgcc aatcagtttc ctattaagta tgttgccgag ttttgatggc
    11341 atgggcaggc atgccattac caagttattt aatgtgatta tgacaagggc tggtattacc
    11401 ttgattatta ccgttgcctt tagtatttcc actatgcttt attcgctgtc agcaagttct
    11461 ccctttttca tgattatgtt tttacaaatt gtgacctttg cagggattta ttttaaactg
    11521 ggcgatttaa tgagcctatt cactttacaa agcaacgact ctcaaaacat gggcagacaa
    11581 gtgtttcgca gacctcgtca aatgatgaat cgacaaacca gaagatttca acgcagtatg
    11641 agtaagattt ttcgtggaaa agcagggtca acgaagcaaa agacaatgac aacaaagcag
    11701 aatcaaacca ataaagctaa ccatactcgt caaaatgaac ggactacacc aaagaaagaa
    11761 acagcacttt ccaatgtgaa aaagcaacaa ggcggtatcg ggcaacgatt aggtgaaaag
    11821 acagcccatg tattggacac gaaaaatcgt ctagtcaata aaagaaagca ggcaaaacaa
    11881 cagatacagg cgacaccaac ccatgtcaaa tatgcccttc atcaaggaaa agagaatgtc
    11941 actcataatg taacagactt taaacaatct atggcagata ccaaagctca acgcaaacaa
    12001 cagcgtatga accagcagga gcaaaaacga aagaacattg ctcaaaagcg tttggagctg
    12061 gataaagcaa aagaaaaaag acgaaaagaa aacccatcat ctcctgttca gaataaccag
    12121 cagcatgttc gcaagcgtca gcaaaccgtc acgcctgttt tgtcacaaaa cactaaacgt
    12181 gaaaatcatc agcgaccatt gaaaaaagag caaaaacaaa agacaccacc gaagcaattc
    12241 aatcagagga agctaaataa aagaacggat aagcgaggac aacaaacatg aagctaaaaa
    12301 taacagtgat ttgtagtgtg gtcttctctc tcttcttttt tctcttatta ttcctagtca
    12361 ttttctttgc agatgatacg gacagtggcg aaaacaacaa ggattcttct attccacaag
    12421 gcggtgtgac cgtttcacca gaagtgttgg cacaccgtcc gttgattgaa aaatatggta
    12481 aggaatatgg gattgaagac tatgtttctt atatcttagc cattatgcaa gtggaatcgg
    12541 gcggtacagc agaagatgtt atgcaaagtt ccgaatcttt gggtttaccg cccaacagtt
    12601 tgagtaccga agagtccatc aaacaagggg ttaaatattt cagtgaatta ttaaccagtg
    12661 cagaacaaca aggagtggat atagattctg ttatccaatc ttataattat ggcggtggct
    12721 ttttaaatta tgtgaggagt catgggaaaa aatataccta tgaattagcc gaacagtttt
    12781 ctaaagagaa gtcgggtggt caaaaagcag actatcccaa tcctatagcc atacctgtga
    12841 atggtggttg gcgatataac tatggaaatc agttttatgt tcagttggtt tcacaatacc
    12901 taaccgacac ttccccaaca gaatttgatg atgaaaccgt gcaagtcatt atggacgaag
    12961 ccttgaaata cgaagggttt ccctatgtct ttggaggggc gtcgcctacc acctcttttg
    13021 attgtagtgg cttaatacaa tgggtgtatg acaaagctgg catttctctc cctcgtgtcg
    13081 cccaagatca gtatgacgct acgcaagaaa tctctatgga agaagcccaa gcaggcgacc
    13141 ttatcttctt tcattcaacc tataatgcag gcacatatgt gactcatgtg gcgatatatt
    13201 tagagggaaa tcgcttttat catgcaggcg acccaatcgg ttatggcgac ttaagcagtc
    13261 gttattggca agaccatctg attggggcta gacgtgttat tcataactaa gaaaggaatg
    13321 aatcaaatga ttataaaaat cgagcgaaaa gaaaagaagg agaaaccaaa gaaatctaaa
    13381 aaaccgagca agcaaagaaa agttccgatg attaaagttg gcacgcataa gaaattaacc
    13441 tttgttttat gggttttatt gattggtagt gtcggatttg gaatctataa aaattttact
    13501 gccattgaca ctcatactgt ccgtgaaaca gaaatcatta aacagcagat tgtcgatacc
    13561 aaccaagtag aaagttttgt gaaatccttt gcgaaagact acttttcgtg gcaacaatcc
    13621 caagaagcca ttgataaacg aaatgaaaaa ttgacccatt atttgacgga agaattgcag
    13681 gtattaaatg aagaaatgat tcgtaaggat attcctacta gttctagtgt gaacgatata
    13741 caggtgtggc aggtttctca agtgaatgag aacacctttg aggtactgtt ttctgttgaa
    13801 caagttatta ccgaagacaa agacaaggaa actatatcgt ctagctttca tgtggttgtc
    13861 catatagatg aatcagataa tatggttatt atcaaaaatc cgacaatgag taagaaacct
    13921 caaaaatcgg actaccaacc caaacagcta gaaagtgacc atacagtaga tacagaaacg
    13981 atggacgaaa tcactagctt tttagaaacg ttcttccagc tctatcctac cgccacagaa
    14041 aaagaattga cttactatgt aagtaatcat gtgttaccta tgattaacaa ggaatatgtt
    14101 ttcgaggaat tggtgaatcc tatctttacc agaaaagata accaagtaat cgtgaatgtc
    14161 gctgtgaaat atttagatca agaaacaaag gcaacccaaa tctcgcaatt tgagttgata
    14221 ttagaaaaac aagataattg gaagattgta aaataacaaa tattggtaca tgattacaga
    14281 tactttgtaa tcatgtactc tttttgataa aaaattggag attcctttac aaatatgctc
    14341 ttacgtgcta ttatttaagt gactatttaa aaggagttaa taaatatgcg gcaaggtatt
    14401 cttaaataaa ctgtcaattt gatagcggga acaaataatt agatgtcctt ttttaggagg
    14461 gcttagtttt ttgtacccag tttaagaata cctttatcat gtgattctaa agtatccaga
    14521 gaatatctgt atgctttgta t
//
LOCUS       DAPCMT010000064        14503 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPCMT010000064 DAPCMT010000000
VERSION     DAPCMT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.064"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..833
                     /locus_tag="RD743_001620"
     CDS             <1..833
                     /locus_tag="RD743_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0563435.1"
                     /translation="FFKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQG
                     WTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRS
                     IHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEK
                     KLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNG
                     LLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI
                     "
     gene            982..1320
                     /locus_tag="RD743_001621"
     CDS             982..1320
                     /locus_tag="RD743_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563436.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1525..2100)
                     /locus_tag="RD743_001622"
     CDS             complement(1525..2100)
                     /locus_tag="RD743_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDU0563437.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2832..3413)
                     /locus_tag="RD743_001623"
     CDS             complement(2832..3413)
                     /locus_tag="RD743_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0563438.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3596..4222)
                     /locus_tag="RD743_001624"
     CDS             complement(3596..4222)
                     /locus_tag="RD743_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDU0563439.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4244..5575)
                     /locus_tag="RD743_001625"
     CDS             complement(4244..5575)
                     /locus_tag="RD743_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDU0563440.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5730..6131)
                     /locus_tag="RD743_001626"
     CDS             complement(5730..6131)
                     /locus_tag="RD743_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDU0563441.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6217..6423)
                     /locus_tag="RD743_001627"
     CDS             complement(6217..6423)
                     /locus_tag="RD743_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563442.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6518..8020)
                     /locus_tag="RD743_001628"
     CDS             complement(6518..8020)
                     /locus_tag="RD743_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDU0563443.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8038..8493)
                     /gene="argR"
                     /locus_tag="RD743_001629"
     CDS             complement(8038..8493)
                     /gene="argR"
                     /locus_tag="RD743_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDU0563444.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8591..9208)
                     /locus_tag="RD743_001630"
     CDS             complement(8591..9208)
                     /locus_tag="RD743_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDU0563445.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9429..10802
                     /locus_tag="RD743_001631"
     CDS             9429..10802
                     /locus_tag="RD743_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0563446.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10877..11989
                     /locus_tag="RD743_001632"
     CDS             10877..11989
                     /locus_tag="RD743_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDU0563447.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(12061..13698)
                     /locus_tag="RD743_001633"
     CDS             complement(12061..13698)
                     /locus_tag="RD743_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDU0563448.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13866..14474)
                     /locus_tag="RD743_001634"
     CDS             complement(13866..14474)
                     /locus_tag="RD743_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDU0563449.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3969 a         3018 c         2612 g         4904 t
ORIGIN      
        1 tgttcttcaa gcaaggaaaa tcagccttag aatattatca acagtataag aaaaacaaat
       61 caaactgtgg tagacgtccg cttgttttac ccgaggaaca atcagaatat attcaaagaa
      121 aggttgttca aggatggaca cccgatgtga ttgttggtcg tgcagcgttt cctattagtt
      181 gttctgctcg taccatttat cgtatgttca aaaaggggct atttgattct tctgacttac
      241 cgatgaaagg caagcgtaaa ccgaatggac atcaagaaag acgtggaaaa caaactttcc
      301 gccgctctat tcatgaacgt gaaaaggatt atagccaatt ctcaaatgag tttggtcacc
      361 ttgaaggtga cactatcgta ggtctgaaac ataaaagtgc tgtaattacc ttagttgaac
      421 gattatcaaa agttatcatc acattgaaac cgtgtggtag acaagcgatt gatattgaaa
      481 aaaaattaaa tcaatggttt gaatctgtac cgaaaaacct attcaaatcc atcacttttg
      541 attgtggaaa ggaattttca aattggaaac agatcagtaa tgtcaatgat attgccattt
      601 atttcgctga tccaggaacg ccgtctcaaa gaggcctaaa cgagaattct aacggattgt
      661 tacgtagaga tggtttattg aaatctatgg atttcaattc agtagatgaa ttttttattc
      721 aatctgtcgc atctaaacga aataatattc ctagaaaatc actggattat cgaacacctt
      781 tggaagtatt tttgagttac gtaagtattg atgatctgtc taacttaatt tgacaattaa
      841 gaaactaaaa aaataatcca agaaacattg aacctatgga tccaaaagct acccaaatac
      901 tatagctaac cccccgctac cttcaatttt gcagataaaa cgctttggga gaaaatttca
      961 aagacaacag gccatcctat catgcccgat tataaacaaa aagctggcta ttccttttgc
     1021 tctgacaaat ttcaattggg aaagttcacg aaactagcag gtgaagaagt ttcaactgtt
     1081 cggatcaaag aatgcccgct tcaaatagaa acattggttc aacgaatttc aactagacaa
     1141 gactttgcta ttgtagaacg tgagatcgta gctatttttg tagaagaagg gattttacat
     1201 gatccaactc atatcaatgt ggataaatgg caacctctta tctataaatt cagagaatat
     1261 acaactgcca accagtcgtt aactttgaat tttagattcc aagaattcaa agagtcataa
     1321 tgataaacat cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa aacaaataga
     1381 agctatacat tagaaaggtc gactgtgacc ctatagatgc agtatatgca gcttatgagc
     1441 atttcttctg ttctctttgt ttttgggatc tgtgctgaag tctttttcgt tttttgccgc
     1501 tctttctttg aaaaatcgat tatgttatac cttctccatt actaattcag gcatctgctg
     1561 agtaatgatt tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt gttcttcagt
     1621 caagacaagc ggcattcggt catggattgg tgcgactgtg cggttaggtt cagtagtcat
     1681 tatgattgat tctgccattt tttcatcctg atggctacga taaaaaccgc caatatacac
     1741 tattttttcc ttatttgaaa acataaattt gtctttttct tcattccact cataaaagcc
     1801 actcattggg aaaacacaac gattttgcaa aaatggttga tgaaaggtct ttttttcttc
     1861 tactgtctct gcacgggcat taatcatcaa ttgtcctttt ttaaatccgg taaatcccca
     1921 cttcatgatt tccggagtga ggatgttttc ttcctctttt cttaacgtca caacaagatt
     1981 tgacggaaaa acttctccag ctgctaattc tgctgcctgc ttcggttgtt tcgtctgggc
     2041 ttctcgataa taattctgaa ggagctctga ctctagatca aaaaaataac gaccacacat
     2101 cctaatccct cccaatcttt ttttctatca tacactgcaa caagaagttc gcaccaacaa
     2161 aacgctttca atttttgtag aactaaaaaa gttgtgacaa gtgacaaact ctccagtgaa
     2221 tggtattctc ctgccaactc ctcggtcaca agtcacaata ttcaacaaac atgagaagct
     2281 gctttttgaa attgttcctt gtgactcgga gtgaaacggc aggcttacga cctctgggta
     2341 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtagaaag
     2401 ccattttcac aaaatttttc ttaatactcg aaactaagcg actttttcac aacctcttcc
     2461 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatatga
     2521 gcagctattt ttatttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
     2581 tccactaaac ggtgttcaat cagctaacct tcggtattag ctcaaaaaac ggcaaaatat
     2641 gaggaactat tttgccgttt gtttctctaa tactcaggtc aaagcgctga tttcacaacc
     2701 tctaatacac ggtgttccag aagtagcttc ttcgaaaaat aagccgaaat ttcacaaaaa
     2761 tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca
     2821 acctctaatt attaaagaac cttttgcgaa gttaacaatc tttctttgat caattgaaaa
     2881 ttctgcctaa gtagtgatag atcctgtgtg actttcaaca ataagatact tccctggatt
     2941 tcagcaatca acgctttggc ttgacggtct gcttcttcat cggacagttt ttcttcagaa
     3001 agtgccagga tattttgttt cgtatacttg acccattgat tgactaattc tagtagcaac
     3061 agacggtatt cttcatccac tgtactcaac tcaataatca aattacctac aggacagcca
     3121 tgataagtga tctgtgtctc atgatcagcg aaaatccagt ccaacatttc ttctaatgct
     3181 tcttctggct gttttccagc atttaagatg tcttcgaaca attctgtctg ccatacgtcc
     3241 atatgcgcat ggatcaccgc tagacaaact tctttttttg aagaaaagta ataataaaat
     3301 tgaccttttc ctgtatcaga agctgctaga atatctcgga ttgacgtttc atgatagcct
     3361 tttttataaa taagatcttt ggtcgcttcc aagatctttt cctttgtttt caatgatatt
     3421 ctcctttttc atttctcata gtcctatatt atacagtaaa ttcatcattt tttcttctta
     3481 atcgtttaaa aaagactgtg ccaaattatc tgacacagcc ctcactataa aactgttctc
     3541 atatagctag atgttcattg attcaagaac tgcgaggaca aaagcaattc ctatcttatg
     3601 atagttgctc taatacttcc tctgttgtca tcacttgatc tgcaataaag ccaaagttcg
     3661 tcaacgctgc atccattgct tcttggccag gagcaccaat tgcatctgtc gctaccacaa
     3721 cattgaatcc actctctacc aattcacgca tatgggaatc gctacacaag tttccgttca
     3781 ttccaccaag aattactgtg tcgattccat tttttctcaa ttggagcgcc aaatcattgc
     3841 tttctggtcc aaagatttta tgaggagagc tgatgatcgt attttcatcc aaataaggtt
     3901 tcaattcttc gataaaatcg gctcctgaac cttcttccac tgattcatat tgactttttc
     3961 tagcaaacat ctgctgctca agcatcatat gttctcctgc accgccaaat tgccatttat
     4021 ggtcatgtgg gtaaaaataa tgaggagaaa taaagagttt gtatcctttt tcttttgctt
     4081 gttttaccat ttcaatcata ttttcgatcg tattgtattt ttctaagata tcttttgtca
     4141 aaccaaagcc accgcctgct ggtttcaaga actcgacttg cgggtcagtg agaactactg
     4201 ctgtattttt attattgaac atggtggatt ctccattcgt tatttatttt aatacatcat
     4261 ttagagcttc actcattgtc ggatgagaat agatttgatc tctcaataac gtataaggaa
     4321 gttttccatt catagctagc gaaatcaagt tgatgacttc atgagaatct tccgcataga
     4381 tacttgcccc tagaatcaag tcagtttcag ggtcgactaa aactttgaat acacctttag
     4441 gatcttctaa tacttgtgct ttagggacac cgcttgtcat atacttgaat aatttgtaat
     4501 tgacgccttg tgctttggct tgtttttctg ttaaacctac attggataaa gtcggagtga
     4561 taaatacact gtaaggcaca gctttccggt tattcgttgt tcgtttattc tcgcccttca
     4621 actgatccat aatgattcga taatcatcta gagagatata agtgaactgc aatccgcctt
     4681 tgacgtctcc aatcgcccaa atgttgtctg catctgtacg aaggtattca tccacagcga
     4741 ttgctccgcg atctgttagt ttaacatctg tattttctaa tccaagtgat gctgtacttg
     4801 gttttcttcc agttgcagcc aaaacttttg acgcttggat tgttacttct tggccatctt
     4861 ctgtaaaggt gacggcagct gttgattctt ggtctgctac ttgatctaca gatacaccta
     4921 gatggaattc gattcccgca tttgtcatgt cttctaaaat catttgcgcg atatcttcat
     4981 cttcacgtgg aagaaattca gaatgggcat caagaacgac aactttagat ccgtattcat
     5041 tgaacattga tgcaaattcc aagccaatat atccagcccc gatgatcact aatttttccg
     5101 gtaattgttc ctgatccatc gcttgcgtag aatcaatcaa gtagcgactt gtattcaatc
     5161 ctttgattgg taaaatgatt ggttcagaac cagtattgat gaaaatccgt tcgccttcaa
     5221 caatcacttt ttcaccatta ttcgtcactt cgatttgatg gttagaaaga aaacgtgctg
     5281 taccgtccat cactgtccca gttgcctcat cgctgatcat atgataattt ttggcgcgaa
     5341 gcatgcccgt caatttttct ttccgagcaa cagcctctgt aaagtcgatt cctctttctc
     5401 cattgaaaat caatgattta gaaggaatac agccgatgtt gatacatgtt ccgccataca
     5461 tacgtgttga cttttcaacg actaaaaccg attcaccttt tgaagctaac gccttcgcta
     5521 aagtcttacc gccctttcca aaaccgatca caatattttg atatttttcc atcatttttc
     5581 cctcgcttct tttttaccta ccgattggta caaaaatatg ataaccgata attgtctatt
     5641 ctccaaatca tctgctttat ttgtcaaaaa aaaccagaca aaaagcggtc ctttcacgtt
     5701 tttagtctgg ttcgatttcc tttattcttc taaacaatcg ttacttctac atcgatattt
     5761 cctctagttg ctttggagta agggcagaat tcatgcgcat cttctgcaat tttttgggct
     5821 tcttcttcgc tgatgccatc gatatgcact tctaatacta caccgatctg atagccgcca
     5881 tcatctaaat tataaagaga gacacgtgct tttactgtgg actctgctgt cattcctgct
     5941 tctttgatca tatgttgtac cgccccattg aaacaagaag cataacctgc tgcaaataat
     6001 tgttctggat ttgttccttc tgcatggatt cctggaggag tgattttcat atgcatcgag
     6061 ccatttggtg cctcgacttc tccattgcgc ccgccatggt tgatcattgc tgtttcgtat
     6121 gcttttttca ttgtactgct cctttcgatt gaccatttgc tacttggcta taattatagt
     6181 gtatgacaag taaggcgctt tctacaaaaa ttctgcttat tcgaatacca gaatgccgtc
     6241 ttctactatt tttccaaaaa acaaaaaaat actactcgtg aacaacagcg tcatatttgt
     6301 tccaaaataa atagcaatct tttctacata atcttttata tgattgattt taagatacat
     6361 atataccaga aagttgaccc cataaaccaa tactgttaaa aaaataacca tattcgttac
     6421 cattttttcc tccttttgct ggtctcccta ccagcactcg atttaaccat accacttttt
     6481 ttgccgtcca agcaactagc ctttcaaaaa gactggttta ttctgctgtt aatgattcaa
     6541 gatcagaaaa atgtccgaat aattttgcta attttactaa cgccaacttg ttctttccga
     6601 attcatcatt ttctatctgt aatactagta acggctcatg taagcttaaa cggatcaaaa
     6661 accagccgtt tccgtatggc tcttctagat catatcgaat accttcttca ttttccgggt
     6721 cgaagttcat ccctttttct tcttccaaca gtcgcttcat ttcttctaat atcttgtttc
     6781 cttgttcgcg tggttcatcg ctgaacaatt ttaatcggat ttcttgtact tctacgggct
     6841 gtttcaactc tttgatttgt tcatctaatg tgataccttt tctttttaat tctggcaaaa
     6901 gcatcaggat ctttgcgatg acatatgcac catcatccag aaaatagttt tccttgaatg
     6961 cagcgtgtcc gcttgtttcg atggctagct gtgtatcttt cccaagacgg ttaagttcta
     7021 atgctttatt gattacattt cgatagccgg aaatataacg aacttggatg cctccttttt
     7081 cttcaatgaa tctttgtaga tgagaagaag taggggaatt cgtcacgatt cttgttcctg
     7141 gatgctcttt taatacgatt gtacttaata ctgcgatcaa attatttcga ttgatcactg
     7201 ttccagaagc actgacaact gcagaacgat ccacatctgt atcgaaaatg atcccaagat
     7261 ctgcttcatt tgctaaaaca gcctgcttga tactttccat cgcttcttta ttatcaggat
     7321 ttgggatatg attaggaaat cggccatctg gttccaaaaa ttgggaacct gttgaatccg
     7381 cacctaagct attcaatact ttttcagcaa agaatccacc agcaccattt ccggcatcaa
     7441 caatgatttt ccaaccattt agtggtttgc tttctagtgt tccaatcccc tttttaattt
     7501 ttgtgactaa atcttgcgca taaggtgtca aaaggtctgc tttttctacg cttccagttt
     7561 gttcgctttt tattttttgc tgctgtgata ggataaaagc aatatcttct ttttctgctc
     7621 ctccttctga ggtaaagaat tttattccat tataataata gggaagatgg ctagcagtca
     7681 gcataaccgc agcatcgcac gagaactgtt caaattgagt ggacataaac atagcaggcg
     7741 tagtagctaa ttcgaaatcg atgacatgta tcccttgttc tttcagactt tggatcaacg
     7801 cctctttcag tgctggtccg cttatcctac tgtcatgacc aatcccaatc gtccattttt
     7861 tatttggatc ggttgatttc ttctttttct ttatccagtt tgctagtccc cagccgatct
     7921 tttttaccat gtctgtcgtc aagtttactt catatccttc aactggtaat gcgattcctc
     7981 gaatatccga tccattttgc agcgctttca tatctttcat ataaaagtcc tcctcattta
     8041 ttccctcatt ttttccagct tttctgcaaa tcgctgtgct tcttcttctg agcggcaaat
     8101 aataatgaat gtatctacac cggccatcgt tccgacaact tcagggtaag ccacttcatc
     8161 tagccaattc gttaccacat cggcaccatt tcgttcagtc aatacgataa ccatgaactg
     8221 aacagactgg atacgcaaaa cctcgcgtct aattgctgta cgcaaacgtt cttcatttaa
     8281 agaaacagat ggctggttaa ataatgtata gcgtactatt ttgttttcat tttgactttt
     8341 tactaacttc atttctcgaa tgtctcgaga aatcgttgct tgagtcacat cggcgcctgc
     8401 ttcatgcagc cgcgcaagca gctcttcctg tgtcccaata ggttgttctg taatgatctg
     8461 gcggatcagt gcttgtcgct gtttcttttt catattagac acctctcaat gttgattata
     8521 gcaaaagata agtaatattt cttcatattt tggtattttt tacttttatt ttttgttttc
     8581 attcaggttg ctaacctttt tctattttaa cacgcaaaat cgtcttcatt ttttcaggtg
     8641 ctgattttct tggatcgctg atgtaaatct ctttgtgttc tttagaagct ctttcatagc
     8701 cttcttttgc gatgaatgct tctaatttct caaacgttgc tggttcttcg gcatagggcc
     8761 cgacgtgtaa gatttgggca caaagacctt cttccatctt tttgaatact agttgttctt
     8821 tcaagtcttc cggaagtttg ctagaacgct gaatggcttc ttgtgctatc gcttctgtaa
     8881 caaagtcagg ctgtcggatc atcaattgat aagaaaaatg ttcttttttc atcactggtt
     8941 catgcaaata cttttcttgc agtccccatt gtccttccag tggatagaca gtatacatct
     9001 gataatcagg aatcagccaa ttttttttag gcgacatgcg tattgcatag ctgactgcat
     9061 atagacaggc tactcttcgt ttaaagtctt cctgattcgg gtcgccgatc cctctgatgc
     9121 taaagaatgg ttttttagga acctctagta tttgcgggct ttttttcata ctatagctct
     9181 tgtctttacg ccattcaatt ttttccattt ttcttcctcc gcgaaattcc atttagttat
     9241 ttattatcat acctcttttt gagtgacttg gctaatcgtg ttgacactat ttttcagttt
     9301 gtgctagaat aagtgaagta aataaatcgt acagataaca cgtttcggcg tgtcgagaga
     9361 gaggcggttt ctatttttat aatagaaatc actctctttt tttacgaaat tttttagagg
     9421 tgatgtaaat gacaaaacaa aaacagctgc ttttagtacc tgcagtaatt gcaacaggga
     9481 tgatgtcttt tgcgggcgta ttgatcgaga cggccatgaa tgtaactttc ccaactctta
     9541 tcagacagtt tgatttaacg actgcacaag tccaatgggt aacaacgatt tatcttttga
     9601 tgatttcgat cattgttccg ctgtctactt atttgaatcg caattttagt ctcaaaaaac
     9661 tctttttagc ttcaaatctg cttttcttgg caggtataat gatcgatttc ttctctccta
     9721 ccttcatggt cctattgttt ggtcgattgc tccaaggtgc agcgacaggt atcgctcttc
     9781 cgttgatgtt ccatattatc ttgacatttg cccccctcaa tcgacgaggc gcgatgatgg
     9841 gtgtcggtac tttgacaacg gccattgctc cagcaattgg ccccacttat ggaggcatta
     9901 tgacttcaca ttttacatgg aatcatatct tcctgtttct tgtgcccgtt ttgattcttt
     9961 ctttgattat cggctggttt gctattcccg agatggcagt acaaaaaagt ggttatttgg
    10021 attggataag cgttttagga atttgtcttt tgtttagtgg aagtctaaca tttttgagta
    10081 cgttgggaac agtttccagc tggattgctt taatcatcgg cggtattggc tggttccttt
    10141 tttactatcg aagtaaaaaa gtggagcatc cactgatccg actgtctatt ctttcaaata
    10201 aaacattccg cttgtttttg attggcttct tagtttatca gtttttatta cttggtgtat
    10261 cttttgtctt gccgaatttc cttcagatcg ttcaaggaag tagtcctttt gtcgctggat
    10321 tagcgatgct tcccggcgca gcggttggtg ctttgctttc tcctttttcc gggaaaatgc
    10381 tggatcaata tggtccgaag aagccaattt ttgctgggct gattctttct ttgattggtt
    10441 ggctagcatt attcctactc ataggacacg cttcagtggg tgtattgatt gcttgtcatg
    10501 tgttttatat gatcggtatc ggattatctt acagcaatat gatgaccacc ggaatgaatg
    10561 cactgaatga agaactacaa ggagacggga acgcaatgtt caataccttg cagcagtttt
    10621 ctggcgctgt ggctacttca ttagtagcgg tcattatcaa ttacgtccag catcatactt
    10681 cccataacta tgaagtgtct actacattag gatcaaaagc agcattaggc gtcttgttgc
    10741 tgttgcttct cgtaagcttt gctcgattcg cctattatct gttctttgct aaaaaagcgt
    10801 agattccata tacggcaggt gttgcatctg ccgttttttt gtgtgaaaat ggttgtttgt
    10861 aaaaggaggc ttttttatgg aaaaaattgc cattatcggt ggcgggatca ttggtatgac
    10921 tctagccagt tacttagata catcaaaatt caatgttgct ttatttgatg aagggacagg
    10981 acaggcaact aaagctagcg ctgggattat ctccccttgg ttgtcgaaac gcagaaacaa
    11041 acaatggtat cgtctggcta aagacggcgc tgcttttttc cctaaactta taaaagattt
    11101 ccaacttgat caaacgattt acgcacaatc agggacactt ttgcttcgac ctgaaaaaga
    11161 tttagctgaa ttggctgttc ttgccgagaa acgaaaagaa gaagcgccag aaattggaaa
    11221 catcgaatgg ctttcacctg aacagacatc tcaagcactt ccgttattga aaccagcagt
    11281 ttcactgcgt atcagtggtg gtggtcgtct ggatggaaaa gcatatttag aacatcttgg
    11341 tcagcgttta gcacaaaaac aaatggagca gatccaggca aaagtcactt tgcataagaa
    11401 gagcgattct tggctcatca agggaccgtt tggagaatac acagctgatc gtgtaatttt
    11461 gacacctggc ccggcactta agcctttatt acaagaatta ggctatgctg cagatatacg
    11521 tccgcaaaaa gggcagctct tagcatttca gacatcttat aagatgagtc aagactggcc
    11581 tgttgctatg cttgacggag aagctgattt cattcctttc atggatggaa agatcctttt
    11641 aggtgctacc catgaaaacg atcaaggctg ggatttggct cctactagcg aagcttataa
    11701 gggattgact aagagtacag aacagttctt gactgatcca gatcagataa aaaactggcc
    11761 atacgagttc cgtgtaggta cccgtgctta tacgtcagat ttttccccat ttttcggcgt
    11821 actgccagat gatccaagct tactggctgc tagcggtcta ggctcttctg gcttgaccac
    11881 aggtccttat atcggctatc tattagccaa cttcttaaat actggtcatt ggtctacgga
    11941 agattatcaa aaggacatct ccacctatat tacgaaaaat ccatcataga aaaacagctt
    12001 tttcaagtaa ggatcccgag cgatattgcg catggattac ttcttgaaaa agctgttatt
    12061 ttagttcaat ttctgaatca aattctccaa actttctttt gcttcatctg gcaagagatc
    12121 ttttcctatc tgagcttcaa tgaatcgtaa acgaatatca agtcgttgtt tcaccagatt
    12181 tttataggac gtatcctcaa aagaaacagg aaaatctggt ataggtaaat agtggatgtc
    12241 ttcaaatgga ccaaacgact tgaattctgc cgtttgttgc acgattcttt caacgattga
    12301 caatgtcgtt tcaggtttga tttttttatc atcgaaatat ccagtattca ggacatagca
    12361 ggctgtttgc tgtttcaaaa acaactctcg aaaagcttga tagtcttccc taagaggata
    12421 gacacggaaa ggattagcaa atggttcaat gactagctca tctagctgtc tttttgtagt
    12481 gatattttcc gcgtctgatc gttttgttgc taatgttgca ccaaaagacg ctgctaaaac
    12541 tgggtcattg atcttgataa ttggcggaag cgaatcatct ttcatgatcc aaaaaacagc
    12601 atcgatcttt tcgctaagat gatcgactcg gtcgggtgtc gcataacgtg atttgactgt
    12661 ccgcccattt ccgtttcgta tatctcctgt tactaggact ttttctcctt tacttgtaca
    12721 tgtcacgccc acattctgac aagtaaggaa agaagaaatg gccggatcat cagacggata
    12781 gtcttgtgtt ttatcaaagt aagaaggttc caaggcagta gcaaaaccat tttttcgaga
    12841 aataacgaaa gcatcgtcat gcaagatcga aacagagtga ccttccgtat gttttgctaa
    12901 ggtaatcgtt gattttccag aaccggacaa cccaaataca gccatcgtat aggtctggtc
    12961 attactagca tattgtttca tcccaccgtg gcaggcaata aagccattgc gatttcccaa
    13021 tgcccaagca agcgtcaaag tcgctttttt taattctcca aagtaacgta atcctagcac
    13081 agccgctgta ttatggagag catcaaacaa aatgagtcca tttggaaatg tttcatgtgt
    13141 ccattcggga tcacaaaaga tataaagatc tccctcatca aaggaaacag acttttgata
    13201 agtctctaag atcttcgaat tcgctggctg aaaattcagc aaataagaat acaaattgtt
    13261 ctcaaatcct tctggaatca gcaaatgact ttgcacagaa aattcttcag ataggccaac
    13321 gattactttt gtatggtaaa actggcgctt gcgactttga tacacagctt ctcttaaaat
    13381 tttctcgtat ttgctttgtt ccttgtctaa gtcctcaaag atccgccgag cacttgctgt
    13441 tcttcccaca atgcttccat cattgaatac tagtgtccta acatcagctg gtaaatctag
    13501 ttcttcagca tgtgagattg ttaaatctgt gatgatcgtt cctggactcc cttttgctag
    13561 ttgataagct gtcttcctgt caaaaacttc atgaacatta ttaccgtaaa atgtcgtttc
    13621 tacaatcgat cgaatactgg ataacagtgg atttccactt gtgatttcgt ttaattcgag
    13681 actctctttt gtactcatgg ctcctcctca ttccctaaac aaaaaaacgt tttctttatt
    13741 atacattatt cgagttttta tttgtactgc tgcgctgtgg gaagcaaacg agttattttt
    13801 cacacaaaaa aaccgagaac ttgaataaac aagtttctcg gtttcatcat tttcctttta
    13861 tcggcttagt accagccgtt tgctaaccag aatgttttag cagcatccca tgaaccataa
    13921 cgagatgtta catattgttc tgctacacgt tcttgattgg cagctgaata gtcgccgttt
    13981 aagtatgaag catctaattg ataacggccg atgtaacgtc cgttagtagc tgtgtatgaa
    14041 ccgcctgatt ctttttgtgc gatccattct tttgcagagc ttgaagttgg cgctgttgca
    14101 gcagctgggg cagcaggagc tacttcaact gtttcaacag gtgcaacgta tgtatgttct
    14161 tgcgttgctg tttcagttgt agatgctgta ttttctgttg ttttgttttc tgttgttgct
    14221 gaagaatcat ttgaagttgg gattgttaat tgttcaccaa cataaatgcg gttgatgttt
    14281 gcgatcgaat ttttttcagc aattgcatcg atcattgagt tgtctcctgc aaatttttgt
    14341 gagatcttag ataatgaatc acctgatttt actgtataaa cttcatcagc gtgagcattt
    14401 gtagttccta caaacattgc agtagcacca gcagcaaaag ttgtacctaa aacaattttc
    14461 ttaaatgaat tcatattaaa ctcctttttt tctccgtttt tta
//
LOCUS       DAPCMT010000065        14326 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPCMT010000065 DAPCMT010000000
VERSION     DAPCMT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.065"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(135..1622)
                     /locus_tag="RD743_001635"
     CDS             complement(135..1622)
                     /locus_tag="RD743_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563450.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRTSVD"
     gene            complement(2177..2740)
                     /locus_tag="RD743_001636"
     CDS             complement(2177..2740)
                     /locus_tag="RD743_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDU0563451.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2755..3798)
                     /locus_tag="RD743_001637"
     CDS             complement(2755..3798)
                     /locus_tag="RD743_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563452.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(3931..4335)
                     /locus_tag="RD743_001638"
     CDS             complement(3931..4335)
                     /locus_tag="RD743_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDU0563453.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4568..5203)
                     /locus_tag="RD743_001639"
     CDS             complement(4568..5203)
                     /locus_tag="RD743_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDU0563454.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5469..6521)
                     /locus_tag="RD743_001640"
     CDS             complement(5469..6521)
                     /locus_tag="RD743_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0563455.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6534..7472)
                     /locus_tag="RD743_001641"
     CDS             complement(6534..7472)
                     /locus_tag="RD743_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDU0563456.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(7923..8573)
                     /locus_tag="RD743_001642"
     CDS             complement(7923..8573)
                     /locus_tag="RD743_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563457.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(8673..9182)
                     /locus_tag="RD743_001643"
     CDS             complement(8673..9182)
                     /locus_tag="RD743_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDU0563458.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(9383..10252)
                     /locus_tag="RD743_001644"
     CDS             complement(9383..10252)
                     /locus_tag="RD743_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0563459.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(10272..11393)
                     /locus_tag="RD743_001645"
     CDS             complement(10272..11393)
                     /locus_tag="RD743_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDU0563460.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(11395..11790)
                     /locus_tag="RD743_001646"
     CDS             complement(11395..11790)
                     /locus_tag="RD743_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDU0563461.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(11809..12615)
                     /locus_tag="RD743_001647"
     CDS             complement(11809..12615)
                     /locus_tag="RD743_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0563462.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(12608..13396)
                     /locus_tag="RD743_001648"
     CDS             complement(12608..13396)
                     /locus_tag="RD743_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0563463.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(13410..13886)
                     /locus_tag="RD743_001649"
     CDS             complement(13410..13886)
                     /locus_tag="RD743_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0563464.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(13958..>14326)
                     /locus_tag="RD743_001650"
     CDS             complement(13958..>14326)
                     /locus_tag="RD743_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0563465.1"
                     /translation="KVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDAIYILQNDLG
                     ISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHY
                     YSVDIPIHEVKYVYDYVKRA"
BASE COUNT         4541 a         2976 c         2168 g         4641 t
ORIGIN      
        1 ttatatgttg taacttcctg ataagaaaac taattaaaag aaagaggtaa ctacataaac
       61 taattaaaaa agctaacttc atagttggag gataaatgga agaaacttca atgattgttt
      121 ctccttgtgt tccttcaatc gacactagtc ctattatcaa tctctctttt ttgtccattt
      181 acttttgcga ctgttcccca ataatcaaat acaggaagcg ttattttttg tcctttagat
      241 tcatgtactg tatagactat tttcgaatcc atcacttgat aaggcatagt atgccattcc
      301 ttgccaattc ttacttcatg attctgcaaa tgttctttgt attgtaatgc ttccgtgggt
      361 gcatagtcat aaagtcctcc gggaaggagt gtcgtgtaac gaataagatt ttcttgattc
      421 attaccaagt gatctctctg agtgacaact ttatgttcat agtttgcttt tgttgcagca
      481 gatacagcca acagtaataa aacaattgcc gttatattaa ctacttttct ttgcttagct
      541 gatgtgctct tcatgaaaac gaacgataaa atcaacgagc caaacagtac ttgaaagcct
      601 aagattcgcc aaggaaattg aatcatttga atgggtgtat tttgtaataa agaccatgga
      661 aaaaatttgg tggataagag ccaagagact accgcactaa ggtaaatcac tttttctttg
      721 cgattaaatc ctttaaaaaa gaagagtgga cagatcaaag aaataatacc aatgattcct
      781 accgtaaagg ttgtcaggtc attattcatc gcaccaatca aactatccga gacatttaat
      841 gcttggtgtt gtaaggtcgt tttatctggc tcattaatcg attgaaccat gatttgtcgg
      901 aacatcggat accaaaagta tgctgttaaa agaatcgtca ttcctactgc ctttgacaac
      961 tccacaatca accggatgct ttcttttttt cttgtttcac ttactacaaa gccgcctaag
     1021 actagtaagc caacggctat tacactcata aaagtggaaa gaacatgtgt ataaatgagt
     1081 aagctcatac tgactgctaa tggaattgca catggctttt tctcataaat cagtgcgtaa
     1141 gcataataca taactatcgg tataaccgta agtgcaatcg cttcagcaat agcagaacga
     1201 tgataaatat caatcgtacg gtacccataa aatgtatata aacagctaaa tataaaagcc
     1261 gcatcgattc tttttaaaaa ttttagtcca taatgaaaca tcagcatgat agtacaaaca
     1321 ttcaatagcc aaacatataa gatataacta acgattaaat tattacttat ccaataaaaa
     1381 atgactgccg gaaaaaaggt caagtaggga taaaaataat tcactccgct cccataatga
     1441 ttaaacgtcg taaaattgat tggaccagaa aaaattgaag ataacccttt gactctagca
     1501 aaatggaagg caatatcccc cgatgtagaa atagtatgaa catttctaaa ataattgatt
     1561 gaataaagtc cagcaataat tgctaggaga atggacaata ttattttttt ctttacttgc
     1621 attacttaat tatatactcc tttcatggat tgctttatct agtataactt aaattttaaa
     1681 taaatcgtta tttattttta ctctcactta aaaaagcact aaaattataa gagagggagg
     1741 taatggtctg aacaccaaga attgttccaa catcgaattg cttaactaat gagttcaaag
     1801 cttaattatg tggctcttat atttaccgag cgtggattgc gatctcttta gcgaaacaaa
     1861 catctttcat aaaaaaaaca gccatatccg ctttcttgga tatgactatt tactactact
     1921 ttactatctt tcattcttct tgtctcactt atgataaggc gctgcgtatc atgagtgaat
     1981 gatattagga aaacttcata aaataaatct tttatacatt tccacttcta gatggtcatt
     2041 catgttagaa gaatcaaact atttttttac tgtcgtaata atattaaaac actagaacaa
     2101 atcaattaaa ttccataaaa tataaataat agacctagca tcatctggta taaaatggat
     2161 gctaggtccg ttatatttac tcaaaattga ttctacgaat agatcttctt atcatttcta
     2221 ttttagtagt catgcgttgt tgttcttcta aaggtggtaa tggtataagt agtttagata
     2281 aatttgtacc atttacattt ggttgcccag ttccgtaaga aattttttct aataacttcc
     2341 aatataatgg cgagtctaag aagcagtcta cgtattctga taataatttc tcaactaacc
     2401 taattcgaat caagtaagaa gcaaaaacag attcctcact tatttcctta accaagaaac
     2461 tttttcccat agttcctcct gtccttgcta tcaaaatgtc attttcttcc aatcttaaat
     2521 caactagttt agaattacta atatcacagt aaggcacact ggaccaattt acacgtccct
     2581 cttgtatatc tgtgattctc aaaaacttaa cattgccttg ttttttcgcc gaatctgtat
     2641 agccatattg aatcttagta gaaatacttc ctaaataaac ccatagtcga acatcgctag
     2701 atgatttttc ttgtcgttct ttatgaacaa cagtgatcat cttgagttgt ccgactattt
     2761 tttagttgca gcaatctttt tgggacgctt atttgcatga agcaacgttg ctaagtctag
     2821 taataaaaca ttggctaaca caggattggc tttctcgcga tacgctgcgg cgccactttc
     2881 aaacaatact gctagacgat cttctacaac aacgatttgt tccaaatacg gtggaaattt
     2941 gattcgtttt aggatccggc taggagagtc ccgctcggac actaagagtt ccgaggtata
     3001 aggaccataa gaacgactgg ctaagagata atggccataa aaagccacac cttgaatctt
     3061 atcagatgtt tttctgctca ttgtcacggt tggttggatt ttgtctttgc ggcgaattgc
     3121 tgttttcatt tcagctatcg tcggcaatag atagaaagtc aactgtcctt caccttttag
     3181 tgtgaagttg ccagatacca gttgatcatt gttttgagtc aaatacgatg cttgtggaat
     3241 ctctgagaga tcagttgtca gccaataagc gattggctgt cccgtctctt cagatgtatc
     3301 tgctaagata tcgatcaaac gtaaagcggc tactcgtcca gtagcagatc ctgtgattgt
     3361 caaccataaa agttcccgtt ttgtatcata tactaagccc cctacatgtg gccgtttagg
     3421 taaaatcagc agtttgatcg gctgtcctgt ttctctatcc agtacatgaa taatagaatg
     3481 atgctgatgg tcatagcagt aggcagaaat aaacacaaaa tcttgagaaa aagccaaccc
     3541 ttgcggcgtc atccattgac aaacctcatt ttcgccggtt tccggcacca ccattcttgt
     3601 ttctgttaat ccaggaatgt tatattgttt tgcttcagcc ttttcccaag ctgcttgaac
     3661 aataggatac cgttttaact ttttttcaat atcctgatgt atccgttgct gcttccaatg
     3721 cttaaacttt aggacatagt aaacaagaag gccaatgacc agcaacacta aaatcattat
     3781 tgctacccgc tgtgtcattt ttttgcctcc ttgttctatt cgatcaatcg tccatatacc
     3841 gctattaatt accgtaacta catgtttctt ttactagcgt ttctttatag gatctttcat
     3901 aatctcattt atagtaacat taagaatccc ttattctact atagtcactt ccacatcaat
     3961 attgcctctt gttgctttag agtatgggca aaatgcgtgg gcttcttctg cgattttctc
     4021 tgcttggtcc ttctcaagtc ctggcaaaga aacttctaaa acgaccccga tttggtagcc
     4081 cccatcttct aattgaaata aagatacccg tgctttgact tcagaatcag actcaagatt
     4141 attttcttcc agcatgtgtt gaaccgcgcc gttgaagcaa gatgcgtatc cagccgcaaa
     4201 aagttgttcc ggatttgtcc cttcactatg gattcctggg cggtcgatct tcatgtgcat
     4261 acttccgttt ggtgcctcaa cttcgccatc acggcctcca cgattgatca ttgctgtttc
     4321 gtacattttt ttcattgaga tcatcctttc tttcgtcgtt ctatattaag tgtaacgagt
     4381 attttttaat ctgacaaagg taatgcttgc attcggcgac atcagttaga caacatcgat
     4441 gatcagtgat agaagcagtt tatctataca aaaaaagaag ggttctatga tatttggtgt
     4501 ccttactcgc cttgagtaac aacaccaaat atcatagaac cacttaatca gcttcacact
     4561 gctttttcta ctttcgtagt tgaaaaaaac ttaaacaaat catcggcaaa tagttaaaga
     4621 aaaaacttaa tcgttgaaag aagcccaatt gcttaaagaa ggcaaactga ctaatcgacg
     4681 gaatccgtgc taatccgtaa agacctgcaa ttagtaaact aataactgtt aaaacaagat
     4741 aaaaatggct aagcgtacct tgaccacttt gacgataaag cagaacaagt aacaatggga
     4801 aaagtagaaa gcctgcatag cctagcccag aacctatatt atggatccaa gtcgatagat
     4861 tacaagagct ttcattcgta ttgatactga ataagccagt aaaaatacaa tcacctatcc
     4921 catataaacc aactgttgca acaacaagta ttgcaagtgt ttttgatgtt ccaacaaata
     4981 ggtgatacaa cgcaggcaac gaaagaacaa aaaaaagtcc agatacaact gaccagacca
     5041 agaaagcacg tctgactgga ctatctacgt caccaaacac actaattacg gtagtcattt
     5101 gattcagttt gggataaaaa aggccaagga tatacggtgt cgataaatca ctcatcacac
     5161 ctaataataa aaaataaaaa ccatattttt gcaaaaaaat cattttcttc ccctactttt
     5221 tagtgattat tcgatacatc caacaatcaa attttttctc attttaacac atatttttat
     5281 tctagttgtc ttttgaacaa tcaataacat aaaggtgagt ttcttctaca ttttaaaaaa
     5341 tcgaatggca ctgaagagtt cagttgtcag catcaatctc ctactcaaaa aggcaaaaaa
     5401 acgtaagtca gatgttgttg acatctcact tacgcatcca ttgattgaaa atttcttagt
     5461 aaaatctact agcctaaaaa tttgaagact tcttctcgat catacagttt aaatactttc
     5521 tcgcataaaa attgagtaat tagtgccgca aaaacaggga tgactaacca acaaagaaca
     5581 acaagaaagc catttagccc tgcatctaaa gaagccaacg gaccaacaag tccgaccacg
     5641 ccgaaaccag cagaagcagg cgtacctgaa atagaaaata gagcgacagg aatagctgta
     5701 atcactgcac taactaaaca aggaataaga atcacaggtt ttctaaatag attaggcatc
     5761 atcatcttca tagcgcctaa agcaatcgca attgtcacac ctgacttatt aaccttccaa
     5821 gagttcacta ctaaaacaaa agtggttgca gctacgccca tcgcagccgc accagcagaa
     5881 acaccatcta gttggattgc cattccgatt gctactgttg aaataggaga gataatcaaa
     5941 aaggcaaagg aacaaccaat caagatagac attaacagcg gttgtaaggt agtgaagtta
     6001 ttaatcaatg atccaattgc agaagtaatt gctgttacaa aagggaataa ataaaaacca
     6061 agtaaacctg cacctacacc tacaacaatc ggtgttaaca caatcgacac tgacccaaaa
     6121 ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg ctgtcagcat cgtattaata
     6181 atatcgcctg taccagcacc aacgtaaaca cccgtttcac ccaaatcagg tgtgaatttg
     6241 actacaccag aaccaacaaa agaagctccg gctacaacca tcattctcat cggatcaaat
     6301 ccaaattgta aggcaatcaa accaccaatg atcaaaggtg ttgctagttg aaaaataact
     6361 gctaactgtg cgatcgttac cgcaaaggga tacgcactaa aatattttaa aatggcacct
     6421 aatactgcat taggaatcaa tccaataatg attcctgttg ctgtaccagc gaggacttta
     6481 ttcagaaaaa ttttcggtga tattttttgt gaagtttcca ttcaatctct cctttatttt
     6541 acgttcaaac tatcttcttt tgcatggaca agctcagtca agaaatcaca ataaggcgtc
     6601 gcaacaccat atttcttccc ttttctagaa attgcgccat tgatataatc gatttctgtt
     6661 aatcgatggt tcttaatcaa gtcttgatac attgaaggat aatgtaaccc aattgtttcc
     6721 gggtcaaaac aagattcaca atgtgcaatg acttctggga catctagttc aatcttctct
     6781 actgctgcta ctttggcaaa ctcattgaca atcgtcgcca ccattttatg agcagtcgat
     6841 gtttttccta gctcagccat attaacgtct aagattgtgc atagtccgtt cattgttccg
     6901 ttcacacatg ctttgcgata aatagaatag tgaatattgt cagaaaaatg agcatttaat
     6961 cctgattcag acaatttgtc tgctaatttt tttgcagctg cttcttttcc atcacctaaa
     7021 ttttgtaatt ctactgaacc acttccaaat aatttgacct gaccaggacc ttcaagacct
     7081 gccgtccaca tggtatttcc aatatagata ttttccattg gtacaaattt ctcaataata
     7141 tcttcatgcc cgataccatt taatagacat aagacctctg tatctttttt gattaatgat
     7201 tggatatcct gcagcatttt ttccagctgc atcgctttgg taaatagaat aatcagatca
     7261 acttgatctt ctttttctac ttcggattga agaacgattg gtagttttgc ttctacctct
     7321 ttaccattaa aatttgcttg taatccatgt tctttgattt gttggacatg ttctgcccag
     7381 ccatcgatta ataatacttc gttcccactt tggtgaagca tcaacccaaa gcgacttccc
     7441 attgcgccag cgcctgcaat tgctattttc attgattctt cctccttttt ctttctatta
     7501 tactcatttg agaatctttt cacaacctcg ttttgttatt cttctcttat ttatcatgtt
     7561 tatatccacc attcaagtta ttttatcagc aaatcaataa aaactggaat agaatcccaa
     7621 tttcatcgta agagaaacta gactagcaat aagaaaatag aaacattcat agaaaaaatc
     7681 tcactcctct gattataaaa gagggggcat taacctcgtg tatactaaaa ccttccattc
     7741 attcgatgga tgattttctt tttgtgaaaa aatattcatt gtagtacgat gttgcgcaac
     7801 acagacggat ggtattgctt attatttatt aaaatcgaac aatacactta tttaaaaact
     7861 tctttcccag attttcttcc atttttacag ttataactag tattatcatg aaaaatagcc
     7921 ttttattgtc tatgggaata ataatttaaa attggtactt ttttattaat tagttcatcg
     7981 cccaataatc tctccagttt cttagcatct cctgactcaa ccggaattat ccaagcccag
     8041 tctaaataat tttttaaaat ctcatcatat tttgcaaata tttcattgcc atattccgtc
     8101 cttaaaatta tattttcagt gccagacaag tgatttatat tgagttgata aaaaagatct
     8161 ccatctaaaa aaatatcatt agaagaagat ttccccacag taatgaccat tccatcagct
     8221 ctatgtgtta catacaaata attgtatgac ctcatttcac ctaatgaatc aattatttct
     8281 tgccctttag ctctaatata ttgatctcta ttacctatct cactcttatt taacaaaagt
     8341 tttttcatgt caaatttaat cggctgtttt atttttgtaa attcgcttct actactaatc
     8401 caatccgaga ttaatttttc aacatgtccc agagtaaact ctgatttatt cgctaaatac
     8461 tttttttgtt gtattaaaga catctttccc cgaagctcaa aattgagcct acatcgattc
     8521 tctatctctt caaaaagcat cttatcttca tcattcttat attcatcatt cataaaacca
     8581 atccagctcc tcgattaaga taatatttta atctcataca aaaactagct ctcatcaaaa
     8641 caaccattgt tttgatttct catgaaattt ctttatactt ttctccgatt ataaattcct
     8701 aaccccttga gaaaattacg catatattta tctccgcaag gcttaaaatt tcgttggcct
     8761 tcttttctca aaaatgcccc gatttcgcct tttgatggat agatttctgc ctcagcaaaa
     8821 acgtctaaaa tatcatcagt ggtcatagac aaggcgattt ttaatttttt tagcaacaca
     8881 ttgttgatat atttgatttc cttgcttttt tgcggcgtgt ctactatcgg cttatctttt
     8941 tgcggaccac gcgccaagag aatcaggcca tttaggaatg cgtcaaaaac ttggttgttg
     9001 atggtttttt catacacatt attttctgca ttatctttgt tcttatcctg tactttggtc
     9061 agtaccgcct gagcttcttc tttagtcagt ggtacaccgc ctagctcaaa agctttaatc
     9121 aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca ataacctatc attgttattc
     9181 atttgttcct ccgaagtatt tagtctttac tttcttacgt actttttgtc ttgctaagat
     9241 ttttaggatc aatgataaaa accgcatcac ttatgataag gcgctgggta tcatgagtga
     9301 atgatattgc tgaatacaaa tagtccgacc acattaagtt gatcggactt cataagcatt
     9361 ttattgacta atactttttt gcttaagaat tgattctaac atcttttgct aaagttaagc
     9421 atcctatagt tcctgcatta tctgctaact taggtgtgac aatgtattct tcgatatttg
     9481 gcgtatcgac atagccgtta attagttcta aaaaacgtct ttgtactttc tttttcagat
     9541 gttcttgttg catgacacca ccaccaaaaa ttataatatc tggtgaaagc atcagggtcg
     9601 tattacaggc acattgtgca atataatccg cctcgatttc ccagaacgga tcttctatag
     9661 aaagatcttg tcccttggtt cccgtacgca tctcgatagc gggtccagat gccaaccctt
     9721 cgagacaatc atgatgaaaa ggacaagtac cagagaaagt atctcctgaa tgtctcttaa
     9781 ctaacgtatg ccccatttct ggatggctaa acccttcaac aaaaatccca tcttgtatag
     9841 ctcctccacc aattccagtt ccaacagtgt aataaacaac actggataaa ccttttccat
     9901 gacctgaaac atactccccg taacaagcag cattcacatc ggttgtccag gcaattggaa
     9961 tttcgaaatg cttattcaac gttccaacaa aatcaaaatt tttccaggca gtttttggag
    10021 tagaggtgat atgtccatag gtctttgatt cacgatggat atcaatcggt ccaaatgacc
    10081 ctactccaat gctttctaat tgttctttat agtgattaaa gaaatcaatt accaaactca
    10141 ttgtttcatt cggtgtagtt gtaggaaaac tcactctttc taaaaccttt aaatcatcat
    10201 tgccaatggc acaaacaaat ttagtcccac ctgcttctat tgagccatac atttcttact
    10261 ccaccgtcct tttatctagg aattcgttct tccctgtcta atagtttttc gaaaaactca
    10321 tgtggttcct tttggtacca ataggcaaca gaagaaatat cgtcttgacg ctcataaagc
    10381 ttgatgtcat catttcctag ggtttggaaa gtaacacgta aattttcttt gaaactaatg
    10441 gggtcgggta aatgccagcg gtacaaccca tgcatcggta aagcatcatc gccaaaacca
    10501 tgaactggat ttggattagg ttttccagca ctaaaataat cccgggtgtg atctctttta
    10561 gattgaaaag gatagcccat aaatagcgta ttaaaatttt gagcatatgc cctttctcca
    10621 aagttctttt tatggaaagc ccaagcgccg ccaaaataat cttctgctcc tgtagatgtt
    10681 attgtaggga aatcttcatc tccatctata taaaacttaa attctccttc cccccaccag
    10741 tatctttcca atgcgcacaa agcaaagtag gttcctacat aatacccagt gcctttgatt
    10801 ccatcaataa gggtgtaatc ttttcctttt gtcgtgatgg gttcacggtt ccatttagca
    10861 tgaaaataca tcgcatcatc cggtacttca gtcaatgcat aattaaatgt ataaaaaatg
    10921 tgcttaataa actcaggatg ttcattcgtt aaagtaatct tagctttttt tcgaaatggc
    10981 atctcaaaat agcaattcat ccctgatgtt ggattcacta cgattggtaa ggaattcact
    11041 tcgcatcttg tcccaaaccc attacaaaaa aaatctccta atggactttc tacagaagga
    11101 gtggattcat catcccaata aatacgcaaa attaaatcac gtaagacgaa actcccagcg
    11161 attgttttgt ctgtcactgt catccaaaaa tgacgtaaaa ttccagggcc ttccatttct
    11221 gctaaagtaa ttgtctctcc agcatttatc ggaacagaag gtgacccttt tctcccaatt
    11281 cctagatgac tcgctgtcat tgccgctttc cctttttcac catttttatt ttctggtgaa
    11341 atagttctac tttcctctcc cttgtggagc catatattat ttaaacaatt cattttattc
    11401 gtcctctatc ctttcttcac ttttttgatt attcatatag atgatttgtt gttgtgcctc
    11461 cgcaataatt gctccaatat ctaaattatc atttcctaac tgcaaagcga ttgacattgc
    11521 caagggaaga ttcattcctg caattacgtg cactctgtca gaaatatagg taaaaaactt
    11581 ttgatttaca cttcctccag ccatgtcagt taaaacaatt atttcgtctg tagtatccac
    11641 taactcttta atttgttcat ctatatcatc aatttcttcg acatatgcac ataagctact
    11701 aatttgatta tgcatacctg ttaaaaactc tagtgtctct ttaaaaccct cagctagtat
    11761 tgaatggcta gcaatgatca atcttctcat aattcaaatc cttttccttt acgctaatat
    11821 tccaaatgca gccataacca tcgcaaaaac aataacgagt agaatcaaaa tggtcatact
    11881 gatttttttc ttactgagta gataatatgc tgcagcagta ataattactg gaattaatgt
    11941 tggtaaaatt ttgtccaaca ttggttggat tttcatacta acttcaccaa aagtgaattt
    12001 aagtggcgtt gaaacactga taactgaagc aattaatgat ccaactactg ttagccccaa
    12061 tactgaagca gcttcagtta aataattaat cttttcaccg tattcagtaa caatcttcat
    12121 tccctgctta taaccaaatt caaagcctct cattcggaaa aagaagatta ccatgattaa
    12181 aatgaaccat aaaaagattc ctacaggatt attttctaaa gccatataag ccgcaattga
    12241 tcccatgatg gttgggatca tcgtcatcaa aagagagtcc ccaataccag caattggacc
    12301 catcgtacca actttaaagt cattaatcgc atcttttgct tctaatcctt gcttttcctc
    12361 cattgcaaca caagcaccta aaataatggc tgccagccaa ggctgtgtat tgtaatactg
    12421 aaagtgatta tttaaagctt cgatgtattc atcatcatct ttgtataatt ttcttaaaat
    12481 aggtgacaat gcgtaagtca cagatgcacc ttgctgtgtt tgataattaa aggtacaaac
    12541 tgacatcaac caacgatagt tcgctttcct aagatccttt ttcgttactt gatatttttc
    12601 gctcatttta ttcatcctcc ccaataaacc caacatcaga agctcccgca gaagcagtga
    12661 tttgtgcatc tttttcagct ctatgataca cattaaatgc taaagcgaat cctatgatcg
    12721 ccgctccaat aataggtacc ttcaaaaaag cggctaagaa aaatccaaca agtaaataac
    12781 taatgtatcg acctgtaggc ataaagcgta acaacatcgc aataccaaca gcaggcaaca
    12841 tttttccggc taatgttagc ccatccataa accaattagg taaaacatcc aaaacgtaat
    12901 taacagcaga ctgtcctaaa gtaacggcaa taaatactgg aattgctgca gataaaccaa
    12961 agaaaattgg ccccaaccac aaaacactat tcattttctt aaattctcgt ttattcgcat
    13021 attcctgtga tttgcgaaca acaaaaccat ttaagatttt gacaattaca tctagttgca
    13081 cccctaacat acctacaggt agtcctattg ctaatccggc ttctactcca agctggctac
    13141 caaatgccgt tgcaataatt gcgcacgttc catagtccgg tacagatgag ccaccaaaac
    13201 cggcaactcc taatgacatc aattgaactg taccgccaac tatcaaacct gtttttatat
    13261 cacccatcaa aattcctgtg aataatcccc aaaaaactgc gtaatttaca aagatcatcg
    13321 gaatcccata ataatcaaca tgtttaataa atgctaatat agtcagcata ataatttgaa
    13381 aaatagtaaa atgcattatc cttactcctt tatcctaatt ttgattgaat ttcctgtgcc
    13441 ttatccgccg gaacaaattg tgtaacaatt tctacaccag aatccaacaa ttttttatac
    13501 gtgctgatat tttcatctga tgcataggct ctagaagata gctgtaatgc attatcttta
    13561 acaacagtac cgccaacaat tagtcggtca attctaatac cgttatctac taaatgaagg
    13621 attgtttccg gctctttcac gatgacaaat actttttcct gatcgtattt ccccttttta
    13681 aaattcgcaa tcgctgtttc ttcagtgata attgatacag ccattccagc aggtctagct
    13741 aaacgcatac ttgatttttg gatgtcgtta tttgcgacag aatcatcgat caccatcact
    13801 ctgtttgctt tagacattgg tgcccattgc gtgacaataa tcccatgcaa taaacggtta
    13861 tcaactctcg acataacaat actcataaat acttcctcct ttatctttca ttttcttaca
    13921 agcaattacc atgccaaaac aaaaagaact tgataaatca agctcttttt acataatcat
    13981 aaacgtattt aacctcatga atcgggatgt cgacactata ataatgttca acgacactga
    14041 aagattgctt aaaatgtgtc tgaaattttt gaaattcctc actcgtttca ttgaaataaa
    14101 tttcatcttc caaagtattt tgttttacta atcgttcaat taagcaacta atgtgaacat
    14161 ataatccgaa acacgtatta ttatcaaatg aaattcctag atcattttgt aagatgtata
    14221 tagcatctga aacccgttct aataatttag tcggatttaa tatggtcaat tgctccatta
    14281 aattcgttag actaaaattc ttcagaatat tattgttcaa aacttt
//
LOCUS       DAPCMT010000066        14107 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPCMT010000066 DAPCMT010000000
VERSION     DAPCMT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.066"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            66..767
                     /locus_tag="RD743_001651"
     CDS             66..767
                     /locus_tag="RD743_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDU0563466.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="RD743_001652"
     CDS             764..1885
                     /locus_tag="RD743_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDU0563467.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="RD743_001653"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="RD743_001653"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDU0563468.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="RD743_001654"
     CDS             3264..4172
                     /locus_tag="RD743_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDU0563469.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="RD743_001655"
     CDS             4207..4479
                     /locus_tag="RD743_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDU0563470.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="RD743_001656"
     CDS             4490..5536
                     /locus_tag="RD743_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDU0563471.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="RD743_001657"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="RD743_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDU0563472.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="RD743_001658"
     CDS             complement(8547..9233)
                     /locus_tag="RD743_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563473.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="RD743_001659"
     CDS             complement(9211..9705)
                     /locus_tag="RD743_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDU0563474.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="RD743_001660"
     CDS             complement(9725..10945)
                     /locus_tag="RD743_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0563475.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="RD743_001661"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="RD743_001661"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDU0563476.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..>14107)
                     /locus_tag="RD743_001662"
     CDS             complement(13021..>14107)
                     /locus_tag="RD743_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0563477.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4554 a         2593 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacaccc cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DAPCMT010000067        13987 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPCMT010000067 DAPCMT010000000
VERSION     DAPCMT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.067"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            6..1643
                     /locus_tag="RD743_001663"
     CDS             6..1643
                     /locus_tag="RD743_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563478.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1788..2528)
                     /gene="truA"
                     /locus_tag="RD743_001664"
     CDS             complement(1788..2528)
                     /gene="truA"
                     /locus_tag="RD743_001664"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDU0563479.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2719..3354)
                     /locus_tag="RD743_001665"
     CDS             complement(2719..3354)
                     /locus_tag="RD743_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDU0563480.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3501..4742)
                     /gene="sstT"
                     /locus_tag="RD743_001666"
     CDS             complement(3501..4742)
                     /gene="sstT"
                     /locus_tag="RD743_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDU0563481.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4940..5938
                     /locus_tag="RD743_001667"
     CDS             4940..5938
                     /locus_tag="RD743_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563482.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5951..7969
                     /locus_tag="RD743_001668"
     CDS             5951..7969
                     /locus_tag="RD743_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDU0563483.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8029..8745)
                     /locus_tag="RD743_001669"
     CDS             complement(8029..8745)
                     /locus_tag="RD743_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDU0563484.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8972..9514)
                     /locus_tag="RD743_001670"
     CDS             complement(8972..9514)
                     /locus_tag="RD743_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDU0563485.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9519..9746)
                     /locus_tag="RD743_001671"
     CDS             complement(9519..9746)
                     /locus_tag="RD743_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDU0563486.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9825..11705)
                     /locus_tag="RD743_001672"
     CDS             complement(9825..11705)
                     /locus_tag="RD743_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDU0563487.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11894..12508)
                     /locus_tag="RD743_001673"
     CDS             complement(11894..12508)
                     /locus_tag="RD743_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0563488.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12650..13210
                     /locus_tag="RD743_001674"
     CDS             12650..13210
                     /locus_tag="RD743_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0563489.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13515..13829)
                     /locus_tag="RD743_001675"
                     /pseudo
     CDS             complement(13515..13829)
                     /locus_tag="RD743_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         4365 a         2721 c         2197 g         4704 t
ORIGIN      
        1 aaaaaatgaa aaaaaacaaa aaccaaataa tagatatcat ttttatgtta tttctaagtt
       61 ttgcttacgt catccctcta ttcatgtcta agggaatcta tcatagtgtc aatcaggata
      121 cttactttca tttatctcga atcataggac tagataatgt atggagttct ccagtaaact
      181 tcaataactt cgatcatcat ggaacaatga tgaatatttt ttatccatgg cttactcttt
      241 atccagcttt tttattttat aaaatgatgg gaaatcttgt tttaagttat aatatctact
      301 atttttttat cactttttta acaatggtcg tttcttattt ttctatgaaa caaataaaaa
      361 acaatcgtta tatttcgctg ttattttcga ttatttatac attttccgcc tatcgtgcca
      421 ttgacatatt ccatcgtgct tcattgggag aagctgtcgc tttaacattt ttgcctttga
      481 tactaatggg gtgttacgaa atttatatta gagattacca aaaatggtac tggttgtcta
      541 ttggcatgac gctagtagtg tatactcatt tactttctgt cgctatggtt tctgtattta
      601 ttggaggaac gttgtttctt tctttttatt tttgggacca aaaaatcgct agactcttaa
      661 gtcttttaaa agctaccgtc ctcacctttt ttttaagtgc gggattctta attcctttta
      721 ttcaacaatc tagagcacaa gaattgaaag tcccactggg aaaagaatta agtggtatgg
      781 ctcctagtga tatgctgaca catattttaa ataataacta taacaactat actattggat
      841 tattcttatt tctcggattg attggtgctt ttatttttat aaaaaaatta actgctgatg
      901 atttatttat tttctttctt ggtgtatttg ttcttttttg ttcaaccaat ttatttcctt
      961 ggcaactttt taaccataca ccagttaaga gtctacaatt tgtctggcgg ttaaatggtt
     1021 tttcttcttt atttatagcc tatacgatga gtatagtgat ctattatatt ttttctacta
     1081 aaaataattc atggaaaata atggtcttta cgtttcttgc actaatattg catctctcag
     1141 gagtaagcaa ttctatccat gctaacaaag attcattaac acttatagca cctgaagacg
     1201 ctgttgctat agctgaaaat tacaatcata ctgattatgc aaacaaagag tcaatctatc
     1261 atcctgatat gattaataat gatcaatatt tactaggtaa ccaggaaata aatccaacaa
     1321 ctcattttat gagtaacaaa cttacaattg aattagataa ttcgaataat aaaaatactg
     1381 tcttgacgac acctatttat cgttataaag ggcaagtggc ttctattaat ggcaaactag
     1441 tacaaacaaa attatctaag tttggcacaa ctgaattaac tattcctcca ggtataaata
     1501 aagtcgttat tacttatcag tacacaaaac tagcaattgc ttcaagatat ttatctatcg
     1561 ttactttgat tttattttta ctttatcgat ttactttttc taaacaaaaa caaccgagaa
     1621 gagaagtaca acattctcac tagattttta tttaagaaag actttttagc tgtacacata
     1681 aacaggctaa aaagtctttt tttatatttc tttaatttgc ttcatacatt atttaacatc
     1741 gatatttata ctgtgaaaca tgcgtttcat ccattatcaa agtattttta tcgataataa
     1801 acttcatcca aaaacagacc ttgtgcaggg acggtttctc ctgcatgttg ccgcactttg
     1861 ctttcaaata tttcatcgat tgtttctatc ggcatggcac ctgtgccgat ttctagtaat
     1921 gtccccatga taatccgcac cattttatat aagaatccgt ctccgacaaa agtaaaatga
     1981 agcatttctc cctttcgttc gatagataat tcatcaatcg tacgcactgt ggattttttt
     2041 gattttttca atgcagaaaa accaatgaaa tcatgtgtac ccactaattt ttcgcaagct
     2101 gcattcagct tgtccatatc caactcttga gggaaataaa agctgtggtt tcgctcaaat
     2161 gctgaaggaa tcacgttgtt ccaaacataa tagctgtatt ttttgcccac cgcattatat
     2221 cttgaatgga agcgctcagg catttcttcg attcttttga tgacaatatc ccgcggaagg
     2281 taacgattca taaagtcgat catttctgcc agtccaattt ctgtgtttgt tttaaaattt
     2341 gctacttgtc ctcgcgcatg ggtgccagca tctgttcttc ctgatccaat aatttctatc
     2401 tcttcatttg tcatctgatg caatactttt tcgatttttc cttgaatggt tttatcgtca
     2461 tctccaagtc tttgccatcc tgaatatctt tttccgtcat attcgattgt caacttgatg
     2521 tttctcatat tttagtggct cctctgtctt ccatttggtc ttctctctac ctttttaaga
     2581 ttgtagcaca aaatatagct ccttgcttct ctaagacaga agctatacaa gaaaactgtg
     2641 gaaatatata tttcagaaaa acaaaaaaca gccaccggat gaagtatccg ctgactgttc
     2701 aatttgacgg ctcaagtttt actgtttgag atgcaagata ctcaacctgg caatgggcac
     2761 ataattaaag aaaaaactga ttctttggca aaaacctatt ttattcagta atggccaaga
     2821 atttatagaa ggaattcttg ccagtccata aatcgccgca aaaagcaaac tgatcacaga
     2881 cagtccaagg tataagtggc ttacgtccat ttgcccattt tttcgataga tcaagaaaag
     2941 aagaactgga aacaataaaa aaccactgta acctaaacct gatccgatat tatggaccca
     3001 tgtcgagaca ttccacgaag attcatgggt attgatactg aacaaacctg taaagataca
     3061 atcacctata ccatagaacg cgatggctgc tgtaactagg gtagctaaca cacgagatgt
     3121 actaacaaat gcagcataca atgcaggcaa tgacaggatg aaaaagatcc ctgaaaccac
     3181 agaccaaata agaaacgctt ttcttacagg actggaaacc tctccaaata cactgatcac
     3241 gctcttcatt tgatctaatt caggataata caatcctaat acgtacggag tcaaaaaatc
     3301 actcagcacg cctaataata aaaagaaaaa accaaattta cgtaaaaaat ccatgatacg
     3361 cctccaataa caattaccta tgcttttaat tgttattata aacgacttta tcttgattta
     3421 acagaaacct ctatttacac gaacatctat ataactcatt aaaaactcga atcattttaa
     3481 aaatactaaa tatctaacgt tcaacgttca cttaatccat ctaaacaatc tttcaatttg
     3541 attttttccc cacttctttt acgcgcgcct agctctccta ttgcagtaaa caacagatca
     3601 cttgacgaat ttaaggcagt ttccattgaa tcctggataa caccgataat aaatccaata
     3661 ccgacgattt gcatcgcgac atcattagag ataccaaaca aactacatgc taatggaatc
     3721 aataacagtg aaccgcccgc aacacctgaa gctccacaag cagctaaagc agacaaaata
     3781 cataaaagga ctttcaaaac aaaaggtgct tcaatcccta aagaatgtac agcagtcaac
     3841 gtcatcaagg aaatggtaat cgcggcaccg cccatattga tcgtagcacc taacgggatc
     3901 gatatggaat aagactcttt tgtcaatttc aatctttcag ccaacatcat gtttatcggg
     3961 atatttgcgg cagaacttcg ggtaaagaaa gcgggaagtg cgctttcttt caaacaaaag
     4021 aaaatcaatg gatagggatt ttctttaata aacaaaaacg tgaggaatgg gtacacaact
     4081 aatgcgacaa acagcatcgt accgatcaaa acaagtaaaa gctgcagata ctctgccaat
     4141 ccagcgattc cagtcgtagc aattgattga taaaccaatc ctaaaatccc tatcggagca
     4201 aatgcaataa tcacttgtac gacttgtgac aacgctatag atatctgatc caccaactct
     4261 tttgtcgatt ctgaacgtgc tcgaaaagct agtcccaaca atacagccca gaataagacg
     4321 ccaaggtaat tcgcatcaat aagagattga acaggatttt gtgtgacatt cgtcaacata
     4381 gtagataaaa cactttccaa actttgcggt gctttttcag atactgtttc ttgtaaagta
     4441 ataggaacag taaaaattga acttacaata actgctacaa gtgcagaaag caatgttccc
     4501 actaaatata agatcaaaat aggcttcacg aatgtcttat tcccaatttt atgcttggca
     4561 attgacgcca tcgtcaagaa aaaaaccaga ataggtgcga ttgccttcaa gcaactgaca
     4621 aacaacgttc ccaatattga aataaatgtc catgtgggga aaaagatacc aaacaatatt
     4681 ccgactaaaa taccaacaac aatacgttga actaacgaca tatccaaaaa cttctctaac
     4741 aaatgaccac cctttcacaa ttttaacaat cattgtatca ttttatgcaa agaaactaaa
     4801 ggtataattt tctaaaaatt ctaacaatac aaacaaccat ttcttgctaa ttttgtactt
     4861 tttctgtcta cacgttgttt tttgagatat tctaagttag gatgttatta atgggcaact
     4921 tatcaggaaa cgttttctta tgtttttgtc caattttttt gtgaggtgtt tattcttgaa
     4981 aaaaataaag aaggagagtt atggcttcct gacaatcttt gtcgcttgta taatgatggt
     5041 tttacagcgt atattgattt cagcagtcac tattcctaca tatgcaacaa accctggtcg
     5101 attctacatc tatacatttt tacaaacttc cgttgttatt ggtgcaaatt tgatcccact
     5161 ttacatgggt taccaatatc aaaaaatcgc ccgtttaaaa gttttacatt atttaagtcg
     5221 ttttgccttt atattcctaa tagccacact agtttgcaat atcttttttt acgtacaagc
     5281 tggcagcttg aatattcgtg attattggct tatcctaaca cctatcagtc agaattactt
     5341 tacttatgct gtttcttgta ctttgctttt ttgtagtatt ccttatacag ttggattttt
     5401 aaataaatta tcgaaaacaa caattaaata tctactatta tttttaacaa ttggattagt
     5461 tggctgctct actctattta ataaagaccc gtttgcatta cagaatggac agagtatttt
     5521 atggattttt tatttatttt taatgggtta tggtttgaaa gaatggaact ggaaagaaaa
     5581 ggtccgctat aagcttcctc aagtatttat cagcttgatt gttttattcg gtttgattat
     5641 tttgatgaca caaatttcac tcatcatcag aggaaataca gatacagcgc tgaggttttc
     5701 taaaccatat tctctcttcg caatgtatta tactttctct tcgtttttat ttttagaaat
     5761 gctatctgat aaattaggta taaaaggaag aggaaatttt cttgctgtat ttttgattgt
     5821 cactcaagtt atatgtaatt tccctttagt ttcttatcgc gtatctacat ttatgaaacg
     5881 tgaatttcca aactcaggga aggcatggct gataaatatc gggcagtttt ttatttgata
     5941 tttattagca gtgtccctcc tagttatctt tttattttat atacaaaaaa tccgtttcat
     6001 tcatcgtttc atcgaatact tcgcttttga ttcgattgaa caattaactc agcgttggaa
     6061 aaaaagaatt cattggcttt ttgttcataa aagttatttt ttagttgcag cattttttta
     6121 ctgctttacc ttcgtgcaga ttttcgtttt agaaccaaaa gaaggttgga aagaaactat
     6181 ccaggttgcc ttaaaaatat ttactcagcg acaagcaccc atgatcctga caatcatcat
     6241 tattatggga tttttcttct tactattgtt gatttccaat cgtttttggt acgccttgac
     6301 tgctaccttg atcattaact tactgctcac tatttcgaca gtaatcaaaa tggaaatgcg
     6361 ggaagagcct gtctttcctt cagatttaaa aatgctgacc ggattgtcag aactattatc
     6421 gatggttagc ccagtcttat taatcgtggg cggattgatc cttctcctat tattgattac
     6481 tagtattatt gtacaacgta gactacaaaa acagtattcc ttgaagattc attggaaacg
     6541 tcgatttatc agtatcgctg tattattagg atgtttttcg ggtgtctttt tcattaatca
     6601 caaaaattcg ccttctttct tgctgtttaa tttatttaaa gtaaacaaga ctttctttaa
     6661 ccagcaaggc gccgtaaaag ataacggacc agtcattcaa ttcctaaaca atatcgacat
     6721 taaaatcatg gaagaacctg ctggctattc gaaagaaaaa attaatggaa taatgaaaaa
     6781 gtacaacaca aaggcagatg aaatcaacca aacaagaagt gactggttaa acaatcagac
     6841 gatcgttctc aatttaagtg aaagcttcag tgatcctgca cgtgtcccta atctgacagt
     6901 acctacagac cctattcctt acatcaaaag cattataaca gaaggaacag gaggattgat
     6961 gttatccgtc ggttatggtg gcggaacagc taacatggaa tgggaaggac tgacaggctt
     7021 gagtatcagt aacctgtctg cttcgttagt tacaccttat acccagctag tggagcgtca
     7081 gaaaataagt ccaaatatta ccgatctatt cgatgaaaaa atagccatcc atccttttac
     7141 agctgctctt tatcgcagaa aacaagtgtt tgataaattt ggttttgaca ggttctatta
     7201 tttagacagt ccggataaat taacttatac ggataaaata gaacaaagcc cacgcatatc
     7261 ggatgattca gcatacaaag aaaccttgaa agctttacaa agcaatacgg aaacgtcaca
     7321 attcatccaa ttatccacta tgcaaaacca tatgccttat gatgattact atagtgaatt
     7381 gaactacact gctgaaggaa ctgctgtcat tcctaataga agaaaagaat tacaaactta
     7441 tatgcaaggc cttcactata ccgatacagc agtaaaaaaa ttcatccaag aaatcgacaa
     7501 aatcgataaa ccgattacct ttgtttttta tggtgaccat ttacccgcaa tctatagtgg
     7561 gaatgattcg aaaaaatatg gattagaaca gcaccagaca gattatttca tttataacaa
     7621 caagtatagt cgtgaacaag cagctacgtt aaataaagaa gttgttgctc ctaataactt
     7681 tggtgctatg gctcttgagc atgcgaatat caaagtaact ccttactatg cgttgttgac
     7741 agaagtatct aatcatctac ctgcattaac aattgatcct acagaaagct tgtctaatcg
     7801 ttttaatgga aaacaggtct ttgttacaga ggatagtcaa attatccaag aaaaaaattt
     7861 aactgagaaa caaaaagaaa ttctagaaga ttatcgtctg atacaatatg atcttgttgc
     7921 aggtaaccag tacagtgcaa catgggcgac acaaaaaata aaagactaac aaataaactg
     7981 agcaaggggt tgtgataaaa gacatgtcac aaccccttgt ttatctcatt aatttgcttg
     8041 tccataaacg atttgattaa agccattttt caattcatta ttcgctgtct cgttgaagta
     8101 aagataatta tcgggattcc cctgattttg ttgtgtcacc aatacttttg gctgattttg
     8161 ctgaaacccg aatagataga cgtgcttttt cagatatggt tctgtctctg catcggaata
     8221 gacggctgtg atttgatagt ctgcttgacc tgtcccactt tctgaccatt ctgcagaaac
     8281 aggcactcca ccaatagcca tcttccactc cccgttcaaa atagcttgag ggactttaag
     8341 accatataag tccacactca tttgattcgt ataacttttg tattgttgtc ccattgtttg
     8401 gccccatttg gtcataaatg cctctaattg tgcggatttg tctgcattcc acagtgtttg
     8461 ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc tctacagaag aacttgaagt
     8521 agatgtttga gttgtttcag tcgttttgga agactctgac gaagtagagg tatctgctgt
     8581 ttctgtactt gctgcagttg acttgctctc tgtgctagtt gttgtagtag ttgcaactgt
     8641 tgctttcgct gtttcttttg ttgattcttt tttcgattca ttcccgttgg tacaaccagc
     8701 taacaaaaaa gctagaccaa ttgttactaa aatccttttt ttcaattaaa aaactccttt
     8761 cgattactcc cacttcttta ttctatcaaa taagcaatgt gattttattg atttttcgta
     8821 ttttctcatc aatctttcaa atcttctaaa aactgagaag cacccgctga tgttgtctgt
     8881 aatacatcag cgggtgcttc ttttatttta atcttcgtga ttttccaatt tacctcacgc
     8941 tttatatttg tatttgaaga aacatagact tttagtcatc atcgtcttcc tcatccaaat
     9001 cttgtcggac agtttttcct tggagtcttt gtacttggta gatgatttct ttattgaaac
     9061 ttaaaagttc aacaagcaat tgttgcaagc catatttttc ttctttttca gctaatttga
     9121 ttgcccgtgt gataaagaca ttttggaaat caaaatcttt tgcaaagttt tccaacattg
     9181 ttgaggctgg taaatatttg ttttgaccat gttcgtaaat ttttccataa cgcatgactt
     9241 cttctaaagt agacggaacg atttcatttt caatcactaa ctcatgagag attttattga
     9301 accatctcgc ttcttgatca gcatattctg aaaaaagggt acttaattct tgcgaagatg
     9361 tacccaaaac atacaattgt gcttggatca atttcatttc ttctaattta aaatttgcta
     9421 atacatgccc caccatggcg ccagcagttg gtttatgatg gtcgatatct gcttgttttg
     9481 tttcttgaac aaatttttct tccactatat tcattgattt acgcctcttt tacttttgct
     9541 gattggactt cataacctaa tttttccaca actgcagcta attgttcagg gtggacttca
     9601 tctggatcaa agtttgcttt gattttgctc gcattaaaca aaactttcgt attgcttacc
     9661 ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac agcttggaca agctagtgta
     9721 tctaattgca taataatttt tttcatcgta gttcttcctt cctttttctt gatagtctta
     9781 ttctagatta cagcttccat aaaaaaattg attcttatca agttttacga tttttcttct
     9841 ttgctttcgt gtccaaaata caataaacgc atcgcgttaa agataacgat caagatacta
     9901 gcttcgtgaa cgaacatccc actagccatg taaataaacc cggcaaataa gccgttgagt
     9961 aaaaataaga cgacaccaat ggcaatcact acattttcac gggtatttaa aacagttttc
    10021 ttagccaagc ggtaggcatg aaccaacgat tcaaaattag attgcatcaa gacaacgtca
    10081 ctcgtttcga tagcaacatc cgttcctgat cccatagcaa tcccgatatc agccgcagcc
    10141 aaagagggag aatcattgat tccatctcca ataaaagcaa cgcgcaatcc ttgttcttgg
    10201 tattttttca caaattgaac cttttcgtct ggtaataatt cagcatggac ttcatcaatg
    10261 cctaacatat cagctacata atcagcagtc atctgattat cacctgtcag catcactaac
    10321 tgttttgctc ctgcttgttt caacttagct aattgttcag cgacttctgg tcgaatcaca
    10381 tctgacacac ctatgatttg agtgattttt ttgtcgattg ctacaataat gaccgagctg
    10441 cctgtttgtt gtaattgatg taaatcttgt acttgctttg gtgtgagaga aagtttgtga
    10501 gcggcaatca atttggtgtt gcccgcacaa actgtatgac catcaatctc agctgtgatt
    10561 ccttgtccct tgattgtctg attatctttg actgctaatt gttgaaaatc aatgtctttc
    10621 tcctgagcat aatggatcac ggcgcgcccc agaggatgat cggataacgt ttctaatcga
    10681 gccactaaag ctaagctgtc cccttcatcc tctgtatagt tcttgatagt ggatacagca
    10741 gtagttcctt tggtcaatgt accagtttta tcaaagacaa aagtatcgat tttggaaaaa
    10801 gtatccattg cttcaccgcc tttgactaag atgccccgtt tcgctccatt accgattcct
    10861 gctacattcg aaacaggcgc accaatgact aaagcgccag gacaaccaag aaccaaaaca
    10921 gtgattgcta aacgaaaatc acgactaatc agaccaacaa ccaacgcaat aagtaaaacg
    10981 gttggagtat aatactgaga aaatcgatca atgaattttt ccgcatggga tttggtatct
    11041 tgtgcttctt ctactaattc gataattttg gcaaaggtcg tatcatctcc gaccttagtt
    11101 gcttcaatcg tcacgtagcc atctgctaaa atcgttccag agaagacttc atctccactg
    11161 attttgacga cttgtcgtga ttcaccagtc actgcggctt catccgcata ccctttcccg
    11221 tcaatgactc gtccgtccac aggaatagct ccaccagttt ttactaagac gatatcgcct
    11281 tcttccacgt catcgatttc cacttcttca gtcgttccat cttcttggac aacactagca
    11341 gtagatgggg ccatctcggt caaagccttg attgactccc gcgttttggt caatgttttt
    11401 tgttccagat agctaccaaa caaaaacaag aatgtaacga tagctgactc cttgtattct
    11461 ccgatcataa aggcaccaag cacagcaata gtaacgagta aatcaattga gataactttg
    11521 actttcactg cctgatacgc atggatcata ataggtatga ccgcaataat agaagctatg
    11581 gctagagcaa tgttatagaa caagacaaaa ccagtaaatt ttccgataaa accaaaaata
    11641 atcaacacag ctgataaggc ggtgatatga tttcgatgct cttggatcca cttttgtaat
    11701 ttcatctttc tccctctttt cctttcattc ttacgagact aagtatagat acttatccat
    11761 cataaaaaat tgaccactat caagtttagt caaaaaggcg gttttctttt ggtagaatac
    11821 actacaaaaa atagttagca agaagactca ctaaaagtga tcttgccaac tattgtcgtt
    11881 tctatcgaaa ttttcattga atgattttcc tgtacagcat acatcggaga tctatttctt
    11941 tatctaagat ttttatcttg ctaatttttt cctttagtct ttcaaatcca cgtcgttcat
    12001 agaattgata cgtacagcca ctatctgtaa aaaggaatat ttcttttcct ttctctcttt
    12061 tttcaaattc tgttagcaac ttggtaccga tgccttttgt tttcaaatca ggatttgcag
    12121 ctaaaaaaat gatctctcca tctggcgaat gcttctttaa atatgctgca agcatttgtt
    12181 catttgctgt ctcgtaagct cctttacttt ctttagcaaa tatattttgt aaaaaatcga
    12241 atatcttaac atatatttgc tgtccaaaag aacgatgttt ctttttttct ccttttatct
    12301 ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa agcaagaatt tgagttgctc
    12361 gattcaactc catatacaaa aaatatcttc cgtaaagatt cagcagaagt tcattttcca
    12421 tatatctctc aaagttcatt ccttcaacag caaaatagat agcttttttg taatctttct
    12481 tttctaaggg tttaaaagtt agtttcatca tttcgcctcc tcaacttcaa tgattgtgtt
    12541 atacttacat tataatagac acagtgtcta ctatctataa tcaatcatct ataagtttgt
    12601 cgattaagcg acagctttgc tttgatatgt cggataagga gataggtaaa tgaaaaagac
    12661 agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa acactatcca cattattagt
    12721 aaataaaaag attcaagcaa tcactatacg cgaactaaca gaaacggcag atgtacatcg
    12781 ttcgactttt tatacacatt ataaggatat ctatgatcta tatgatcaat tagaaagcaa
    12841 cttttttcgg gatctaaatg aaattctctc ttatgatcca gcccatagct atgaggaact
    12901 atacactcgc ttgattgact atgtttacgc aaatactttt ttatatcgct tatttatcct
    12961 caaccatggt gatatttcgt ttcagaagaa agttaaacaa gttattgaag aaaactatct
    13021 gaacatatgg ctatatgaag acaagaaaac gattatcaca gaagaaatga gattctttac
    13081 tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg tgggtagaaa gcaattacaa
    13141 gtactccaaa aaagaattat tagaactctt acagaaactg aatgatcaca tagagaaaat
    13201 catgccttga ataaaaacat actaagctta gttaagaaga accgtaacct tctcgtataa
    13261 caactgaatc ttatttagcc aaattgttta ctgaagattt tttgagtaaa atttctataa
    13321 ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc ctctctaagt gatatatctt
    13381 gttgtaccaa tcaaattcac ctcatcatta ataaattact tttaggctaa tttattacaa
    13441 cttataggtt gaagctgtaa ccaatttata gtcatattat atttttatca tccagtttat
    13501 tgaattcttc tcgtttacct caattcattt tcaaggggaa tcagctcatc atctaacatt
    13561 ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gtttcgcttc tggatgtttg
    13621 gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg aattggatct
    13681 tcacctggca tatattgcag attagaagca tgtaattttt tctcagaaac aaaccactca
    13741 ttggaaaaga catgaatcaa atcatcactt acttgtgtaa aatagcgacg tttaatgaca
    13801 aagatatctt cattgctatc tagtgtacct tcatttcttt gtgcttaaat tcaaagataa
    13861 ttttctcaat aaagattttt gattccagta agattccttt actcctttta caacatagcg
    13921 ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca ttaaaaatag cccaattctc
    13981 tataaag
//
LOCUS       DAPCMT010000068        13850 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPCMT010000068 DAPCMT010000000
VERSION     DAPCMT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.068"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(243..605)
                     /locus_tag="RD743_001676"
     CDS             complement(243..605)
                     /locus_tag="RD743_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563490.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(679..1059)
                     /locus_tag="RD743_001677"
     CDS             complement(679..1059)
                     /locus_tag="RD743_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDU0563491.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1123..2316
                     /locus_tag="RD743_001678"
     CDS             1123..2316
                     /locus_tag="RD743_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDU0563492.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2393..2977)
                     /locus_tag="RD743_001679"
     CDS             complement(2393..2977)
                     /locus_tag="RD743_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDU0563493.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3181..4179
                     /locus_tag="RD743_001680"
     CDS             3181..4179
                     /locus_tag="RD743_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDU0563494.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4291..4800
                     /locus_tag="RD743_001681"
     CDS             4291..4800
                     /locus_tag="RD743_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDU0563495.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4830..5489)
                     /locus_tag="RD743_001682"
     CDS             complement(4830..5489)
                     /locus_tag="RD743_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDU0563496.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5491..6255)
                     /locus_tag="RD743_001683"
     CDS             complement(5491..6255)
                     /locus_tag="RD743_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDU0563497.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6263..6940)
                     /locus_tag="RD743_001684"
     CDS             complement(6263..6940)
                     /locus_tag="RD743_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDU0563498.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6977..8362)
                     /locus_tag="RD743_001685"
     CDS             complement(6977..8362)
                     /locus_tag="RD743_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDU0563499.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8572..8656)
                     /gene="ffs"
                     /locus_tag="RD743_001686"
     ncRNA           complement(8572..8656)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RD743_001686"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8679..9194)
                     /gene="tadA"
                     /locus_tag="RD743_001687"
     CDS             complement(8679..9194)
                     /gene="tadA"
                     /locus_tag="RD743_001687"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDU0563500.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9301..10158
                     /locus_tag="RD743_001688"
     CDS             9301..10158
                     /locus_tag="RD743_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0563501.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10239..10574)
                     /locus_tag="RD743_001689"
     CDS             complement(10239..10574)
                     /locus_tag="RD743_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563502.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10641..12011)
                     /locus_tag="RD743_001690"
     CDS             complement(10641..12011)
                     /locus_tag="RD743_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDU0563503.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12089..12625)
                     /locus_tag="RD743_001691"
     CDS             complement(12089..12625)
                     /locus_tag="RD743_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0563504.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12934..>13850
                     /locus_tag="RD743_001692"
     CDS             12934..>13850
                     /locus_tag="RD743_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0563505.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMT"
BASE COUNT         4233 a         2773 c         2257 g         4587 t
ORIGIN      
        1 tcaacgctgt taaatttatc atgtatttct tccttggtca actataaatt aagcattact
       61 ttgtgaaatg atttttcgat caattattat tattcgttat acaaccgttt tcaacatatt
      121 ttgaaaataa aatgcactaa aaaactatct ttagcagaac atttcattgt attttttgga
      181 ccaaacaata aaaaaagaag tttgaaaaaa attcaaactt ctttgttcgc ttaatctgta
      241 ttttagaaga aaggcactcc ctctacttcg taagttccaa gaacagagcg catttgacct
      301 ggttcttccc aaatatatcc ttcgcctaat tcttctcttt gattcttttg ttcagtttca
      361 aagacaaaca acggaacatt ttcctcttta atatgtgtat ttgtcaattc catcaaacta
      421 atcttgctaa catcgatatg aactatttct tttaagaaat taagtatatt cgctaaacca
      481 gtctttcctt cagaaagttc cgctaacgca aattccaact tgtcatcaat tgaatggacc
      541 aaaaattttt tagtaccatc ctccaaatgc aacataattg taccagcaac tcttttgctt
      601 gccaaaattt ccacctcgat caacttgttc tatggcttac ataaaaattc tagcacattt
      661 tcagaaaaaa tacacttttt atttcttatt ttctttccgt tctagttcag ataatgcttc
      721 ttctatccaa ataggtaact gctccgcaat agtggtaaat ccgatcttac gatttttatt
      781 cgtgaaatac ctttttctgc gatatggaac agctggattt gttttttcta acgttcttaa
      841 agataaactg atttttttgg aatattcatc aatgtcgatg atctgaactg ttactggctg
      901 tccgactttt aacaatgaat ggatattttt cgtataaccg gattgtactt ctgaaacatg
      961 aataagtcct tgcgtctcgt tatcaagaga aacgaatgcg ccataaggct gtatccctgt
     1021 cacttttcct tcaattattt gtccaatttt ataagtcatc tacttccctc tcttcgcatt
     1081 tatcattata ctgtaaatag ctcaaaaaaa gaagcgacga aaatgactgg aggtcttaag
     1141 atgatcgaat ttgataaaca gatttcaaga aaagatacag aaagtgtaaa atgggacgca
     1201 attgctgaga catatcaaat ggatgactta cttcctttgt gggtagcaga tatggacttc
     1261 ttgtctcccg gtggggtagc taaagcgttt agtgaataca tcaaacatgg aatctttggt
     1321 tatgccactc tgtctgataa actatatcag gctatcattc attgggaacg ccaacaccat
     1381 gctgtggagt tgacgaaaga aaatatcgtt tttacaagtg gtgtgttaac cagtttagct
     1441 gcagcagtac aaacttttac aaaaccaggc gattctattt tgattcatga cccagtttat
     1501 cctccatttt cctctattat agaaacaaat caacgtcaca tcgttcgaag ctctttgcta
     1561 gaaaaaaata atcatttcat catggacttg tctgacatgg agaaaaaaat cgaagaaaac
     1621 aatgtaaaag caatgatttt atgcaatcct cacaatcctg gagggcgtgt ttggagcaaa
     1681 gaagaattga aacagttaag cgaattgtgt ttaaaacatc aagtgattct gtttagtgac
     1741 gagatccatc aagatttaac tttattcgat catacattta cttccatgct aacgattgat
     1801 cctaatttag atgatctact agttgctttt acttccgcaa ccaagacttt caatctagct
     1861 gcaatcaaaa attcaatggt atttatcaaa aatccagaga tgaaagctga ttttgaaaaa
     1921 catttagtga tgaaccaaca gcacgaaatc aacacttttg gactgattgg aacccaagcg
     1981 gcttatgaga cgggtggaga atggttaagc caactgattc cttatctaga aaaaaatgtt
     2041 caaacagtta atcagttttt tgaacagtat cttccgaaag tgagaataat gaaccctgaa
     2101 ggaacttact taatgtggtt agattttacc gcctatattt cagacgacca agaattggag
     2161 aacactcttg ttcggaaagg aaaagtcgta ttaaatcctg gcatcacgtt tggtccttcc
     2221 ggtcatgggc atatgcgctt gaatattgct tgtccagaag aaactttact ggaaggactt
     2281 aatcgaatca agtatgcttt agagacaatc gattaaatca tttattcaaa ataaaaggac
     2341 gagaccttac taggtttcgt cctttttcat aattactccg taccatacca aactattctg
     2401 aaatttcaat tgtttcaatc actacatcat gtacaggtcg gtcttgtgga ccacgttgta
     2461 catttgcgat ttcatctact gtgtccatcc cttctagtac atgtccaaag actgtatgtc
     2521 ggaaatctag ccaaggagtt ccgccacctt tatacgcttc aatgatttct gatgggaatc
     2581 cagcaccttc tagttgtcca agcatattag ctggcacatt tgtgttattt acaataaaga
     2641 attggcttcc gtttgtgttt ggacctgaat ttgccataga taaagctccg cgaaggttga
     2701 acagttcacg actgaattca tcttcaaaac tttcgccata aatactttcg ccacccattc
     2761 ctgttccagt tggatcgccg ccttggatca tgaaatcagg aataacgcgg tgaaaaatca
     2821 cgccatcata ataacctttt ttggaaagtt caatgaaatt ttgaaccgtc ttcggtgcta
     2881 gttcagggaa taattgaaca gtaatgtcac cacggtttgt tttgataaca gcttttggtc
     2941 ctttttcact ttttaaatct agttgtggga atgccatatt gtttcctcct aatatttgca
     3001 tacttcataa taataataat aacataatac ctaacctctc gtaaaaatac tagatttgtt
     3061 ttcttttatt tttcatcact agatacattc tataacttac cgtttcgatt tcgaattcaa
     3121 acatttttga gaagcattct caattaaatt ctgctatagt gtgaaaaggg aggaattttt
     3181 atggaaatct atgacattac aatcatcggt gcaggtcctg tagggatgtt tgccgctttt
     3241 tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca gtctacctca acttggtggt
     3301 caattagcaa ctttgtatcc cgaaaaatat atttacgata ttcctgggta tccagcaata
     3361 aaagcaagtg aattaatcga tcaattagaa aaacaattaa ctacttttaa ccatacattc
     3421 catttaaaag aagaagttct ctcgcttact cgtgaagacg aagttatcga gattactaca
     3481 aataaaggca tccattattc caaagcagtc attttagctt taggaaatgg ttcattccag
     3541 ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa accacggatt ggattatttc
     3601 gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg tggtgattct
     3661 gctatcgatt ggacgttgat gttagagcct attgcaagcg aagtttactt gattcatcgt
     3721 cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc atctgtcaac
     3781 ttgcttactc cctatctcat cgatggatta tcaggcaata atggggaact gacagatatc
     3841 cggttaaaaa aagtgaaaag tgacgagacg attgatttga tgattgattc tctgatcgtc
     3901 aattatggat tctcttctaa tttagaacat ctttcttctt ggggacttga tagtaccaga
     3961 aatgccatta ctgtaaaatc agatatgtct acttctattc caggcgttta tgcagctggt
     4021 gacatctgta cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct
     4081 actgcagtga acaacgcttt acactatatt aatccaaagg aacgaactca gcctggtcac
     4141 agcacaagct tatacgataa aaatcttcgt cctagttgat tttttatatc tctttgcatt
     4201 tcaagttaaa cccgaaagca gtttcttgca tttttttcat gttccgttaa catgaaatag
     4261 agaaagaaaa aataaagggg gagcaataat atggtcgtca aaactgagac tttagaagaa
     4321 aaggcaagag aactattgaa ggaacgtggc gtaactgtag aagatatcgc acaacttgtc
     4381 atgtttttac aaaaagacta tatttctgat ttgactttgg cggattgtac tgaaagtgtc
     4441 aacagcgtgc taacaaagcg tgaagtacat aacgccatta tcactggaat ccaattagat
     4501 atattagcag aaaagaaaaa attgatgagt ccgttgcaac atattatcga agatgacgaa
     4561 ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc
     4621 ggctttacga attatggtta tatcgacaag gtgaaacctg gtattttaaa agaactaaac
     4681 agccacgatg gcgttcatgt ccataccttc ttagatgata tcgtcggagc gatcgccgct
     4741 gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa
     4801 ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt ctttcttttt tgtttgtttc
     4861 aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata attctaataa tacaaaaaat
     4921 aagataaagc tatgcatgaa aaattcttct ggaagtacat tgatgattgg atcagtaacg
     4981 ggatcgaata accagtcatc gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga
     5041 tcaaatccta tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc gatttgaaac
     5101 ggacgaatca accgccaaag ccgatcattc ttttgcaaat atttgataaa caaaatactc
     5161 gggatcaaag tgatgaaaaa cacagcataa tccactaaaa acaaaatttt cacttcataa
     5221 aagtgatggg ctccgctagc agaaactggg aaatctggca agctgaggat ggttttgaag
     5281 ggattattga gataattcat taggtgatca aaatttttca gtaacgtctc ttgatctaaa
     5341 gaagtataat ccaaaatttg caaatgtccg atatcaaata cgtatagtgg tcgaaagttg
     5401 atcgtcacac caactgccag actgatcagt gtcagaaata ggctgaacaa tccagcatat
     5461 tccatccact gccatctcga atttttcata ttagaagctc cattcatcca gtgattgttt
     5521 cacataggta gctgggatag gcaacccagg tacatcttct tcttttgtaa aacctgataa
     5581 aacgagcaaa gtatctatat tattgcgtat cccagcttgg atatctgttt catagttatc
     5641 tccaaccatg attacttctt ctttttgcag ccctaatact tcaacggctt tatccataat
     5701 gatggcttct ggttttccga tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga
     5761 gatgactgac cctgctccag gaagaaggcc gcgttcagta ggtatattct tatctgggtt
     5821 cgttccaatg aaagtagcac ctttttgaat agcaagtgtt gctttaacta ctttttcata
     5881 ggtcagataa ttatctaagc ctaccactac atagtcagga gcctcttctt cccatgtaaa
     5941 tccggcagct aaaatcaagt caatcaatcc tgcttctccg ataacataga cttttttccc
     6001 ttttccatct gctttcataa aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg
     6061 agctacatga atgtcaaact catctgctag acgattagct actgtttctg gtgacttagt
     6121 cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg
     6181 tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat caattaaata
     6241 tcctttatag tccattcatc cactactctt ccctttttct gatcgtaaat gctgtttttg
     6301 ttttttctac ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt tttgctgact
     6361 ctgtttttcg atttttaata accggttttt tgcgattaga tgcgactggt ccttttttct
     6421 catcgatata cgcacgatta tggttttttt tcttttttcg gcgctgagtt gtcttttctt
     6481 ttttaccgcc gattctctcg atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa
     6541 gatagtcttc caaggaatcg attcgctgat ctggatgcgc ccgtcgatta tccgcttgga
     6601 aaaatccttt taggcgtagt tgttcataac cccaatctcc tacgatataa tcatatcggg
     6661 ctagtacatc actgtaacgt tctccaagct tttctgcatt aaatccttca cgataatcgg
     6721 acacaagccg ataatgtctt tcacctatca aaaaattact gtcatctaac agtgtcacta
     6781 gctcgccttt gactttttct gctttaggct cctcaacgat ttcttccaaa gcagctgtca
     6841 gttcttcggt gactgttgtt tcctgttcag ctgatgattt tttcttagca atttctgttt
     6901 tatgctcaac gttctgctgt tcattttgtt ttgcagacat gcgttcacca cctatctttt
     6961 ttattatacc ttgtttttat ttgattgggt agtgagcttt caagtaatct cctaccacac
     7021 ttcggataaa ttcagggaac aaaaattcat tttttccagc aatctcaatc gttggaaaaa
     7081 atggaacaaa catcaaatga tctacggtag taaaagtata atttttattc agatcgattg
     7141 gcttgttctt ccaaaagact tgatggttaa tagaatcata tgtgattcca ttatagacca
     7201 tctgcccaaa aatcttaccg cgaaatccca ttcccttcat aggatagtta cgtaaaaagt
     7261 tacgattttt ttctatttct aaaactaagc ggatcagatc atttccaccc aatgtgacat
     7321 ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg atcctgattg acccgccctt
     7381 ttggaatttc agttaaaaac agaccactgt ttaatattgc tgcttccgtc tttcctcgtt
     7441 ctttcaccgc ctcaaaggct acttggataa acgaatgctc attgaagata tctagactca
     7501 gagaaaatgg caaatctgct acttcttttt cttttaataa ttcatgccca cgttctagat
     7561 aactatttat ttcttgttca tcttctgtaa aagctgtcat tgtttcggta gggatggctt
     7621 tggctgaatg cttaaggatt tttttctctt catcgatggt taaatggact tcaccgacat
     7681 actgcccgta ctttccagcg gctgctattt ggacatcatt gattacgtga ccttctctga
     7741 ataaatgatg ggtgtgcgat cctaaaataa catcaattgc tggtatttct tttgcaattt
     7801 ggaaatcatc ctgaatcccg agatgactca tcaaaactaa aacatccact tgtgaacgta
     7861 aatcttcgac taattctggc aaaatatcat aaggattccg tatgtcccag ccattcggat
     7921 tataagtaag cggaaatgga gcagtaaatg caattagtcc gattttcgtc tggtgtttcg
     7981 ttgtaatgat tttatatggt ttcgtccatg ccggtggctg gagcgtattt ttatcgaata
     8041 agttatccag gatgatatca aactcagcat gatcgtataa atgattcaac tcttctttgg
     8101 aatttcctac gccttcattg tttcctatcg tcgctgcatc ataaccaact tcattcatca
     8161 gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga tccacaaaat
     8221 cacctagatc tactgcaatc gttgtcgttt tttctttctt ttcgttttct cgtttacgtt
     8281 gatccaaaaa ccttctgatc cttggccaat tttctaaatg agaatgcaga tcattcgtat
     8341 gtaaaatcac tattttttcc atgtaccctc tcgcctctct ttatctttat cataccatag
     8401 gcagcttttt cttccattga aacaagctct atttcagagt ttttcctgcc ttgaagcaaa
     8461 ttgtataagt acaatattcg ataaatcagt ctggctaaaa aatcaggaac attcattttt
     8521 acagtactat aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc attataatca
     8581 gacacatggc ggaacctgct tagtgctgct tccttccaga cctgacacgc ttcacaagcc
     8641 caccattgtc ctagcctttc ggcaaagaat agtatacctt atttttcatt cgttgaccag
     8701 tcttttttta atttcttttt tcgctggcgg agctttcgga aaaagtcagt caaaatcatc
     8761 ccacattcgt cttctaaaat gccaccttct acatatgctt ggtggttaaa ccgctcgtcc
     8821 tccaaaagat tcatcaaggt accggctgtt ccgccttttg gatcatacgc gccgaaataa
     8881 acttctgcta cacgagacaa aatcatcgcg ccgctgcaca taggacaagg ctctaaggta
     8941 acaaaaagtg cagcatcttc caagcgccag cttcccactt tttcacaggc ttccttgatt
     9001 gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt atatcctctt
     9061 ccaatcactt ttccatccaa tacaaccact gctccaatcg gaacttcatg aagcatttct
     9121 gctttctttg cttctgccaa tgcaaggcgc atccattttt ctttttctac tgcatcaaga
     9181 tttgtattta ccaattaccc ttccctgcct ttgttttttc ttgttttact ttagcatatg
     9241 ataaaaaggc tgtaactaac ttttttttct atggtaaaat aaatacaaga ggtgaacaag
     9301 atgaaaatgg ataaattact tgctctttac ccacaagcac agaaaaaaaa ctctccagca
     9361 tcagaagcgg atatcctttc ttttgcaatt gatgatgctt ttatctggat caaacaagat
     9421 tcattatctc aacaagaaac cagccttttg aaagcgttat tccctgtaat caacgatcaa
     9481 aaaaaacacc catggtatcg ctatcttttt aaagatgctt cttgtgttca agaaaaaagc
     9541 tttcgtttga tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa
     9601 gatactattt tggaattatt tcgctattct gctgatttct ttttttatac agacacagat
     9661 gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg ctactgaaat caacggtatt
     9721 tttctatctt tagatgctga ttttgatacg acaacgtctg tattcgttgg ttctttccat
     9781 tcacctggca ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt tttaacagag
     9841 caaaacaatc tttatactca cagccgtact ttttctatgc aagatgtggc tttacattac
     9901 tatacaaaag atactgtaaa agatagtaag ttgatcaaaa gttacataga actgatcaaa
     9961 caaaatgaaa tggaagtgat cattcaagaa ttatggaatg aattaggcaa tgtcagctct
    10021 gctgcaaaaa cactgtttct tcatcgtaat actctaaaat atcgaatcga aaaatttcaa
    10081 gaacagtctg gtttcaatct aaaaaaagcc aacgatttac tgttttgcta tcttttattg
    10141 cttcatgaac ataattaaag atagattaaa ggggttgtga caataatttt gtcacaaccc
    10201 cttatttcga atgaatagtg ttcaaaagct agtttctctt acatcatttt ttcaacaaaa
    10261 tgcattagcc ggtcttttac atcttcggtt gaatctttga ttgaatcacc ataaacagag
    10321 attttctttt ttcctgattt gatttttgct aacattccca tcaaagaatc caagtcacta
    10381 tctgaaagac catctacgat ttctagtaat ttttcatttc catcaaattt gtcatagacg
    10441 aattttttta ctttcgtccg agtagtcata tgatggatct tttccattac ttttcccgat
    10501 acaacgactg ctgtcgctac actggcagcg gctgctacgc ttaatccgat tgttactttt
    10561 gttgatgttt tcataagtat cagctccttt ttatctatct ccatcatagc atagacaaaa
    10621 aaataaacga actaatccac tcagtgaaat ctcaatcctt tagggaaaaa gttctttact
    10681 gttcctttta cttgtttccc aaatccgaca ggtatcttat ctaatactag caaaacccat
    10741 ccttgattcc catcacgttg aaatgtttct cctgctgtgt aagattgcca ttccttttga
    10801 gtgatcggca agcaaggtat attctctatc ttttttgtag caagcgctaa tgcatagcta
    10861 ggttcaaatc gattcttttt gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa
    10921 ccatcgagag aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt
    10981 cttccagtag cttcataatg aaaatcattt gaaaactccg tccagagttt ttcctgctct
    11041 tttgtcattt gcactttact tttttttcgt gtcttctttt ctttatgcat ctgattttgt
    11101 ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct gatctttatg aggccatata
    11161 cgtattgttt tttccaaacc agctacgctt ccccattcag aacgtcctga actaacggat
    11221 tgagtcaaag gaatctcctc aatcgttact ggataatttt ctacaagcca gctaatgatc
    11281 tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt
    11341 agcattttta ttgctgaaga caaaatctct tgctgacgtt tttgacagta tagcggagat
    11401 tcttctgtcc actcttttat tgcatttgga tcttttcgga acattccttc tcctgaacaa
    11461 ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt
    11521 gcatgatttg tcactattgc gttcgaaact ccccatcgtt ctatattttc tgataaaatc
    11581 ttcgcacgtt tcggaaagat ctcattcgtc actaataacc ctttcccctt catttgagca
    11641 gctaattggg ttgatttacc accgggagcc gcacacaaat ccaaaacttt ttcaccgggt
    11701 tttgctgcag cagctgttcc aacgatcatt gcacttggtt cttggctata ttcataccca
    11761 gcttgatgga ggaaagactt tccattgaca gtgccaagaa atccctcatt gctgtaggga
    11821 gcgggttgta attcttcgct atggtaagtt tggaccatgt ccaatcctgc cggttttagt
    11881 ggattccacc gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat
    11941 gcttcttcgc ctaaaagaag acgatatttt ttgataaatt gctgtggtaa cgttgtcgct
    12001 tcctccttca ttccaatgtt tcgttagaca ttttacaatc ttttttacga aaaataaagg
    12061 tggctgatct actttttcat gttgctgcct aaggtgactg ttcctttaag atattttcca
    12121 agtattcatc cgtcacctcg tttttatccc aaacagcgtt catcatttca agaaaatctt
    12181 gagaagaatc atctgagcgc aaacttccca atgcattaag ccataccaat tcaagcgact
    12241 tttcttcttt gatcttcgtg tgacctttga catatgagaa aaatggccgc ttaaatagcg
    12301 aatccatgtc taaatcagat tttgcaatga tttcacgaat actttcatgg atagaaaaaa
    12361 aatctttcaa taagtagaca atgcttaaaa catcatcgga actgcattca gctaagtagt
    12421 tttcaaaata aacagtgatt tcttctaata tcttctcttt taacccgctg attgatttga
    12481 aatgtgtata aatcgggtat gtagaacaac cgcagaactc tgctaactct cttgcagtaa
    12541 ttgacttcat tccatattct tccacgtata ccatactttt gaacagcaaa tattctttac
    12601 tataatttgt tactctcccc atcattctta cctccgtctc ttttcatcta cccataaata
    12661 aataacataa ataattctaa aaatattatt gataattatc aaaacatatt tagttatatc
    12721 cattaaaaat caatttttcc gccttaaaaa caagaatatc ttgacaaaat accctaggaa
    12781 ataaacaata gaataaacaa ggtaacaaaa aaattaaata acgttatata aaaaagaagg
    12841 cgacactctg tgagaataac acagactgaa atgactttcc actcacagtt tttgtctaac
    12901 tatatacaaa aacagaataa aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag
    12961 ttacttgttc tacaaatggt cattcaaaaa aataattgtt ctttacaaga attatccaat
    13021 gacttacata ttccaaaacg gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc
    13081 aagcaatttt tattgattga tgaatttatt tattctaatc ataaaggaga gatccaaacc
    13141 aaagaaaccg atcaaactaa aaagttagct gttttttcta agttaaaatt atgctaccta
    13201 aaagaatcca atcgttttaa ctatttgatg ttactaatca attatccaca aactgctgta
    13261 ccaaaaaaat atttacttga acaactttat atatcgccct cctatctcga aaaactgaca
    13321 aaacagctca atcattcctt aaaaaaattc catttacgga tcctatctgt aaaaaattgc
    13381 tgtactcttg aaggaaatga gttatctatc cgtctctatc tttacttttt tctttcagat
    13441 acatttggag gtaacgattg gccattttcc tcacttgaat taaatgcact aaaaatgaac
    13501 agtacagatg agcaaataga aaaaatttca aaacaagaaa aaaatatcta cttgctgtta
    13561 actttattca tattacgaaa caaacaacag gcatattttc caaaaacttc ttctgaaatc
    13621 actgaattga tggacttatt gcagcataca catgattatt cagaatactt tacttctttt
    13681 tcagtgagtc gcccttgtga agagacagaa acagagagag cctactttaa ttattttttc
    13741 tattgttttt ttccaaatca aatctctttt cgacaaaaac aggagattgg ttttttattt
    13801 tccacaagca aaaaccttat ttgtcgacaa gtcggtcaat tgatgactta
//
LOCUS       DAPCMT010000069        13795 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPCMT010000069 DAPCMT010000000
VERSION     DAPCMT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.069"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(22..882)
                     /locus_tag="RD743_001693"
     CDS             complement(22..882)
                     /locus_tag="RD743_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDU0563506.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            974..1402
                     /locus_tag="RD743_001694"
     CDS             974..1402
                     /locus_tag="RD743_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDU0563507.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1564..1740
                     /locus_tag="RD743_001695"
     CDS             1564..1740
                     /locus_tag="RD743_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDU0563508.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            1767..2213
                     /locus_tag="RD743_001696"
     CDS             1767..2213
                     /locus_tag="RD743_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDU0563509.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(2367..3746)
                     /gene="brnQ"
                     /locus_tag="RD743_001697"
     CDS             complement(2367..3746)
                     /gene="brnQ"
                     /locus_tag="RD743_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDU0563510.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            4451..5422
                     /locus_tag="RD743_001698"
     CDS             4451..5422
                     /locus_tag="RD743_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDU0563511.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            5446..7638
                     /locus_tag="RD743_001699"
     CDS             5446..7638
                     /locus_tag="RD743_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDU0563512.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            7655..8131
                     /gene="ybeY"
                     /locus_tag="RD743_001700"
     CDS             7655..8131
                     /gene="ybeY"
                     /locus_tag="RD743_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDU0563513.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            8115..8510
                     /locus_tag="RD743_001701"
     CDS             8115..8510
                     /locus_tag="RD743_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDU0563514.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            8525..9424
                     /gene="era"
                     /locus_tag="RD743_001702"
     CDS             8525..9424
                     /gene="era"
                     /locus_tag="RD743_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDU0563515.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            9567..10379
                     /gene="recO"
                     /locus_tag="RD743_001703"
     CDS             9567..10379
                     /gene="recO"
                     /locus_tag="RD743_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDU0563516.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            10763..11680
                     /gene="glyQ"
                     /locus_tag="RD743_001704"
     CDS             10763..11680
                     /gene="glyQ"
                     /locus_tag="RD743_001704"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDU0563517.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            11682..13757
                     /locus_tag="RD743_001705"
     CDS             11682..13757
                     /locus_tag="RD743_001705"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDU0563518.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         4647 a         2478 c         2892 g         3778 t
ORIGIN      
        1 ttaagaaaaa gacaaaaatg attaatcgct tttttctgtt gtttcagttc cataataact
       61 atgatcttct aggtctaaag cattcaaaat aagcgaagcc gtttcttcaa tagataaaga
      121 tgccacattg atcactacac atcctagttt ttcataaaga tcattcgcaa aatcgagttc
      181 agctctaatt ttttcgatat cggaataagc agtatcaggg tttaatccgt aagagcgcat
      241 ccgttcttta cggatattat ttaaaatatc tgggttattc gttagtccca cgattttttt
      301 aggatcgatt tcccacagct gtttgggaat atgtgcttgg ggaatcaaag gtaaattagc
      361 aacttttaaa tttttatttg ctaaaaacaa gctcaaaggc gttttggaag ttcgggatac
      421 gcctagcaaa acgacatctg cttctaaaaa accacgagga tctttcccat catcgtattt
      481 tacagcaaat tccattgctt tgatccgttt gaaatagttc tcattcaata aatggagggc
      541 accaggttgc cgaattggct tgataccagt taaacgttca atttctgata tcggtggcgt
      601 catcacatcg aaactaaata attgatgttc ttcacaaaaa gaacgtgtta tctcgatcag
      661 ttcttgattg attaatgtat gcaggacaag tgctttttct tctaatgcat cttgcaatgc
      721 ctgccttaat gttttttcat ctgtcacgaa cgttctgcgg aataggttga actcaacggt
      781 aggatactga gccatagtag cttgcgctag ttttgtcgca gtttctccag cagagtcgga
      841 gatgacaaaa aaagtaacga tttctgtaga atcggaagac atggataaaa cctcacttgc
      901 atttttttta tgtttattat accatactct agaatgcgta cttcgtaatc attttgattt
      961 ggaggaataa gtcatgaaaa aacagacatt gatcgaacaa tcattagaaa cattgaaaaa
     1021 taacggtttt aaatatacta aaaaaagaga agcattgtta acttatttag tgaagcgtaa
     1081 ccgctacgta tctgctaaag aagtttttga ctatatgaat caggaattca aaggggtaag
     1141 ttatgatacg atttaccgga atctccatga ttttgcagag atcgatttac tggaagaaac
     1201 cgatttaaat ggtgaaatga agtttcgttt tcattgctgt cagggggaaa tcggccatca
     1261 ccatcatttt atctgtacag tgtgcggaac aacgaaagaa atccatatgt gtccaatgaa
     1321 ttatttcgaa gaacagctag gtggatgcac gatcgaaggg catagattcg agattttcgg
     1381 aagatgtgaa aattgtcaat aaggatttaa ataaaataac atttgataat ttaaaaaaaa
     1441 agcaagattt ccttgacggt caaaacagca ttagatataa tatttacagg acagtcttgt
     1501 tttttgagat atatttcaat aacaattacg gttacttttt aagctcggag ggagggaatt
     1561 aacatgtcaa aaacagtcgt tcgcaaaaac gaatcacttg acgatgctct tcgtcgcttc
     1621 aaacgttccg tgtcaaaagc gggtacttta caagaatctc gtaaacgtga attctacgaa
     1681 aaaccaagtg taagacgcaa gaaaaaatct gaagcagcta gaaaacgcaa aaaattctag
     1741 ttctgtgatg aattggagtt ggtagtgtgt cactactaag tagattaaac gatgacatga
     1801 aagcagcgat gaaagcgaaa gataaggaat ctttacaagt catccgaatg atcaagtcat
     1861 ctatacaaaa cgagcagatt aaagaaggtc acgacttgac agaagaagaa gaactaacgg
     1921 tgttgtctcg cgaaatgaaa cagcgacgtg attctttgca tgagtttgaa gaagcaggac
     1981 gtgacgattt agctgaaaaa gtaaaaagtg aaattgtaat agtcgaaaaa tatatgccgg
     2041 aacaactttc cgacgaagaa attcgacaac ttgttcaaga agcaatcact caaactggtg
     2101 cttcatcaat gaaagaattt ggcaaagtta tgggtgcaat tatgcctaaa gtcaaaggaa
     2161 aagcagatgg aaaccaagtg aatgcgattg tgaaagaact attacaatct taattttatc
     2221 atttaaactg cggacagtga ttgtccgcag ttttttgtgt tcgtaaaaaa agaactaaaa
     2281 aacagctaga agaaagaata agattctttc ttctagctgt ttttttcctg tttaatatct
     2341 cttatgttaa attgtgtttg atttggttat tcttctggta gttcatttgc ttcttgttta
     2401 taccctgata ggcgataggc gtaataaaag agtaagctgc cagcgtaacc aagtaacgtt
     2461 gggaccatcc agccaagtcc taatgcagca aaaggaacgt gatgatgata aaatcctagt
     2521 aagcttgcta cgacagtttg ctgactcact aatgcaggcg aatttgccaa catatccaat
     2581 acggctggaa cggcagaaaa gaacatcgtt acttgataga caaatggact cttttggaag
     2641 aattttgctg ttaaggaaag caagattaac gcaagtgaca atggatacaa aagcatcaat
     2701 actggtaaag accattggat gatattatcc aatccggcat tcgcaacgac aaaagagaca
     2761 aaagtcgtta accgcagcca tgtcatataa ctgactttag ggaacagttt atggaaatct
     2821 tgtgcaaaag aaacaacgag tcccatagcg gtagtgaaga cacctaatgt gaccattacc
     2881 cctaagaagc tagcaccgaa gttacctaaa taaaaattag tgatctgaga aagtgcagtt
     2941 ccgccatttt ctgataaagg catcttattc aaactcatcg caccgagaag gaccagacca
     3001 aaataaatca tgacttctaa cgcaacgctc aggctaccag cttttgctgt taattctgtg
     3061 acttttttat ctttaaaacc aagatcacga actgcatgga caaaagtgac acttaaagca
     3121 agtaaagcta ctgcatcaac cgtgttgtat ccttctaaaa cgccatttgt caaagcgttc
     3181 gtctgataag aatctgcagc agattggttt agattcccca tgggttggaa taaagaaacg
     3241 acaaaaatga tagctaataa tgcaagaaac aaggagttta aatactttcc gacatatttg
     3301 accaaacgtg ttggatgtac agtaaggaaa taagcaagta aaaagaaaag agcagagaat
     3361 aaaaacatac ctaatgaatc atatttttca ggcaaaaagg gtttagcagc catttcaaaa
     3421 gcagttgctg ctgtccgcgg tgtaccaaag aatggtccga tcgttaaatg aacaaggatc
     3481 aagaacaagg cagcatacca ttttccgaca ggtttagcta agtcatacag accatcactt
     3541 tttgtcacta ctacagctaa gatagctaat aatgggaaaa gagcgcctga gatggcaaat
     3601 cctacaccag cgctgatcca gtttgatcca gccatttgac ctaagtggac aggaaagatc
     3661 agattacctg acccgaagaa catcccgaag ataagtgaac tgaccatcaa taattgcttc
     3721 catgaataag tttttgtttc ttccataaat ttttcctcca ataattatca ttttgagtaa
     3781 caaaataaac gttgcagaaa ctagaaatgc cactaatatc agattccatg aaaaccacca
     3841 ccttaaaata taaaaaaagc ccttttccac ttgggaaaag ggctctagat tctagaacgg
     3901 taccaccttt tttcgcttaa aaaagcttct tcattcacgg acgcttttcg ataaagttat
     3961 ccgctagcct gataatggag gcagccacaa tgatctacgt tttcgacatt gactcggaaa
     4021 tgatgttcgt ctattcgatt tattgtcatt tctcacttac atgactcact gggaataaat
     4081 cgctgaatag cctactgttt tcctcgacgc tttgctgtat atttatttgt tgacgttata
     4141 atacacctgt tttttttgaa atgcaagaat atttcaaata ttttgttttc tttttttgaa
     4201 acaagaaaaa tacccagaga atacttgcta agttagacgg aatacttgtc ctctatctta
     4261 tgcatagctt acatgatcag ctttttttat tcaaacgaga cctttagaga atggaagcag
     4321 ctatttttta acgtttcttg gtacttatgg ttaagcaact cttttagaac cttttcttat
     4381 ttgctatata gtttcttctg agtatggtaa aataggaaag aatgaatcga aaaaaagggg
     4441 atgtctattt ttgacggaag agtctagttc tttagagatt agattgacgg ctgctgatga
     4501 tgtcagcatg cttttaggtg cacacgacaa gcatataaaa ctgattgaag aaacaacaga
     4561 aacaaccatc catacacgtg gagaaatcat tcagatcatt ggcgaacaat cccagatttc
     4621 cttggctgcc tcagtcattc ggacattgca ggaattgatc aaacgaggaa tccatgtaag
     4681 tacaccagat gtcgtgacgg cactgaaaat ggggcaaaaa ggcacattag attattttgt
     4741 tgaaatgtat gaggaagaga tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa
     4801 cagcggccaa aagaaatatg tcgaatcggt agccaaacat gatatcgtat ttggcgtagg
     4861 accagccggg acaggaaaaa cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa
     4921 aggtgaagtg caaaaaatca ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg
     4981 ctttcttccc ggggatctaa aagaaaaagt agatccttat ctgcgtcctg tttatgatgc
     5041 tttataccaa atttttggat tagatcatac gaaccgattg atggaacgag gcgtgatcga
     5101 aattgctcct ttagcttaca tgcgggggcg gacattggat gatgcattcg ttattttgga
     5161 tgaagcacag aacacgacag ttgctcagat gaaaatgttc ctgacacgtc ttggttatca
     5221 atcaaaaatg attgtcaatg gagatacgag tcagattgat ctgcctcgag ggacaacaag
     5281 tggtcttgtt catgcagaac gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt
     5341 tgaagcaagt gacgttgttc ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa
     5401 ggcagatctc catcaagaat aagctttgct taggaggata agcacatgtt gaataaaaca
     5461 ctgcagaaac tacaagaaaa acttggaaag gcagcattgc ctttcctgct gttcttgttt
     5521 tttatcatta tctgcggcgt tacatttagt agtgtcaaac aaaaggccaa tgatttcaga
     5581 gaaggccaag tagcagaaga aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa
     5641 accgaacaaa aaagaaaact ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa
     5701 gatcttgcag atgaccagaa caatcggatc aagcaactat ttgaattgat cgataaaacg
     5761 aatgatgcca tcaacaaatc ttatgacgaa aagattgata aggcaaagga agatgaaaat
     5821 atcccaaagc ctactacaga agagcggatc gccgcgttga agaaaaattt tgaaaatgtg
     5881 aatgcagaaa atgttgcgtt ttatcaaaaa ctaccgaaca atttttattc gacgatattt
     5941 gaaatgactg aaactgagat ccatactgtg agggatgaga gtctgaagct cattgacgaa
     6001 cagatgagca aacaagttcg tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag
     6061 caaatccaat atttgaatgt cacaccagca caacaacaaa tgattcgcta cttagtggat
     6121 caaggtatcg tagtgaatga cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc
     6181 gcacgagaag ctgtacaacc agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt
     6241 aatcaaatcg atgcttcagc tatgaaaaaa ttggaattac taggactgac taatcaaaca
     6301 acttcgattt ttccgctaat tgccatggtt ttagctgttt tgcttcaaat cggcgtactg
     6361 atttataatt cacttcaatt ccaaaatgca ggagaaagaa caaaatacgt gcttttctat
     6421 gtgacagcta tgtcagtcag catagtgctg atgaaatttt tccaattatt ccaaacggaa
     6481 caagctactt atatcccgct gttttatccc gctgcttttg caccacttgt tttaagcttt
     6541 ttcttgaacc gaagagcagg gatcatggct ggtattttcc aagcagtatc tgcacttttc
     6601 attttttatg attcgattgg cacgaacttt ctaacgatta tcttgatgtc ttatgttttc
     6661 tctgggctca tggcaacgat tgtcagacgc aaacggatct cagaacaagg attatctgct
     6721 gccatgtggg tcattatttt tcctgtgatg atggatgtca ttttgatcgt ctatcaagga
     6781 atgagctttg gtgatgcaac gacttggtta atgcttgtct gcgggttcac tggtgcactc
     6841 ttctcattct tgctgacgat cggtctacac ccatatattg aattgatggt caatgatgac
     6901 agtgtaattg tattaaatga actcagcaat ccaaatcatc ctttattgaa acagttgctg
     6961 gaagaagcgc caggtactta tcatcatagt atgatggtag ctaatctaag tgctaacgct
     7021 gtagcagaaa tcggcggacg ttctttgcta actcgagtag cttgctatta tcatgatatc
     7081 ggcaagatca aacatgctag ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg
     7141 cacaatttct tattgccaga agacagtaaa catattatct ttggacatgt gacagatgga
     7201 gccaaaatat tggaagatta taatatgccg caaatggtga tcgatatttg caaacagcat
     7261 catggaacca cgctaatgcg ttatttctac atcaaagcga aagaacgcaa tccggaagta
     7321 acagaagagc aattccgtta tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc
     7381 aatattgctg acagttgtga ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa
     7441 atcgaaagtt ttgtccataa tcttatcgaa gagcgtattt ccgatggtca attagacgat
     7501 agtggactga ccttgaaaga aatccgaaaa gtggaaaaat cattgatcag cggtttaagt
     7561 agcaccttcc attctcggat caaatatcct aaaatgaaaa cagaagttga gaagataaag
     7621 gaagaacaag aagaatagaa agaagcgatg tataatggat attaccttta tggatgaaac
     7681 aaaccaagtc tcagaagaga aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc
     7741 ttatttaaag ctgccagagg aaacagaaat gtctgttact ttcatggaca atgcggcaat
     7801 ccaagtaatc aatcgtgatt accgtggaaa agatgcaccg acagacgtca tcagttttgc
     7861 tctggaagaa gaaggcgaag acgaaatccc agtgattttt gaagatggag agaatccatt
     7921 gcctcgtgta ctaggagaca tcatgatttc tattgaacgt gctgctgaac aagcagaagc
     7981 ttacggccat tcatatgatc gggaacttgg ctttttagcg gtacatggat tcttgcatat
     8041 caacgggtat gaccatatga caccagaaga tgagaaagaa atgtttggtt tacagaaaga
     8101 gatcttggat gcctatggac tcaaaagata aaaacgtaga gaaaaacaaa catttcctga
     8161 tgtctgtgga atttgctgtc caaggagtca aaaccgtttt tgaggaagaa cgcaatatga
     8221 ggaaacatgt tgcgttcggt atccttgctt tatttatggg cgtgattttc cagcttgatc
     8281 ggatagagtg gttatggctg cttttagctg tttttctcgt ttggatagta gagatcatca
     8341 atactgtttt tgaaaatgta gtggacatgt ttacggattt tcattttcat ccgattggga
     8401 aaaaaatcaa agatatggca gctggagcag ttttagtgac cgcttgtttt gctgtcatca
     8461 ttgggttgat tttatttgtc cctaaaatat atcatttgtt gtttaattaa aaaggagaac
     8521 gtatatgcca gaacataaat cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa
     8581 atccacatta ttgaaccgaa tcgtgggtca aaaaattgcc attatgagtg acaaggcaca
     8641 gacaactaga aataaaatcc aaggagtata tactacacca gaagctcagc tgatttttat
     8701 cgatacacca ggaatccata aaccaaagca tcgtctagga gattttatgg tagaagcagc
     8761 ttacagtgcg ttgaaagaag tggatgcagt cttatttatg atcagtgctg atcaaaaacg
     8821 cggccgggga gacgatttta ttattgaacg tctaaaaaac gtgcattctc cagtttatct
     8881 gattatcaat aaaatcgata agatccatcc agacgagttg ctgggaatca tcgaagatta
     8941 ttcgacgcag atgccttttg cacaagtgat tccgatctct gctacagaag gaaacaatgt
     9001 cgaacgctta atggatgtat tagtcagtga aatgccagag ggcccacaat attttccaga
     9061 tgatcaagtg actgatcatc ctgaatactt tatcgtttct gaattgatcc gtgagaaagt
     9121 cttgttttta acacgagacg aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa
     9181 gcgaaacgag aatgataaaa tccatatcca ggccacgatc atcgtcgaaa gagatagcca
     9241 aaaaggaatc attatcggca aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag
     9301 aaaggatatc gaaaatcttt taggagacaa agtatttcta gaactttggg tcaaagtcca
     9361 aaaagactgg cgagacaaaa aagtctatct gcaagacttt ggctaccgaa aagacgaata
     9421 ctgatattct gaacttctta taggttaagt atagaaataa actggataaa aatccgcacg
     9481 ttgagaaaaa cggcggaaga tcgaaacata aataatctgg atcggcagaa tctgcttttc
     9541 cagatttatt ttttaggagg gagaatatga gccagcttgc agaatcgaaa ggcttgattt
     9601 tatctagccg taattacaaa gaaaaagata aattagtcaa aatctttacg gaatcttctg
     9661 gcaaaatgat gttttatatc aaaggaatcc atcggcaaaa tcagccatta gctccggctt
     9721 tgctcccctt taccgaagca gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt
     9781 taagcagtgt caaagacgtc catgcgtttc ggaacatcca agaagatatc tttttgagcg
     9841 gttatggtac ctatttgcta aatctagtag atgcagcaat cgaagatcat caatatgacc
     9901 cgcatttatt ccaatttacg caacaagctc tgcagcggat ggatcaagga gatgatgcag
     9961 aaatcatcac gaatatcttt gaagttcaaa tactgcaacg cttcggaatc gcgccaattt
    10021 ggacgcactg tgtcgtatgc ggggaaacaa aaggaaaatt tgactattct tccaaatctg
    10081 gcggtgtgat atgcgagaaa cactggccaa tggacgaaca ccgttatcat gcagatcctc
    10141 gcgctgttta tttcgttcga atgttttcgg ctatttctta tgataagatc agtggaatcc
    10201 acttgaaaga agaaacgaag caggcaatcc gtgacctcat tgaccagttg tatgatgaat
    10261 atgtcggtat tcatctaaaa agcaaaaagt tcattgatca aatgaagtct tgggcacacg
    10321 tattaaagac gccagagaaa aatatgcctg aaaaagagat acctgaaaaa gaagaataaa
    10381 tgaaagatac aacaaaaaaa tcaaaactat ttgacaaata cagggacttc cgtctattat
    10441 taaccgtaga ttaaatagat cgttcactga gaggatctat tatataaaca agaaaactgc
    10501 aaaatgaata cgtacattga ccaagatcag taaaaaacac gtggtttagc gagttcggga
    10561 gagtggaagc cggacccaaa cgatttttga agaggcactt gcgagtacga taaatgaagc
    10621 tgccgaatct ttattcggaa gtagggtgga accgcgaaat tattcgtccc tatgtctgta
    10681 atcagacata gggacttttt gtcgttttct ttcctgtatt cttattgaca gcaacgctta
    10741 aaaattgaaa ggggcaaaac agatgagtaa aaaattgacc gtccaagata tgattttaac
    10801 attacaaaaa ttttggtcat caaacggctg catgctgatg caagcttatg acacagaaaa
    10861 aggagcaggt acgatgagcc catatacatt cttacgggca atcggaccgg aaccatggaa
    10921 tgctgcttat gtagagccat cccgccgacc agctgacggg cgttatggag aaaacccaaa
    10981 ccgtttgtat cagcatcatc aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca
    11041 agagctttat ttagaaagct tgcgtctttt aggaattgat ccattagaac acgatattcg
    11101 ttttgttgaa gacaactggg agaacccatc gatggggtgt gccggtgtgg gttgggaagt
    11161 atggcttgat gggatggaga tcacacaatt cacgtacttc cagcaagtcg gaggactacc
    11221 ttgtaaacca gttacttctg agatcactta tggactagaa cgtcttgctt catatattca
    11281 agaagttgaa agtgtctatg atctggaatg ggctgatggt gtgaaatatg gcgagatctt
    11341 caaacaacca gaatatgaac attccaaata ttcttttgaa gtaagtgacc aagacttgct
    11401 actaagcaac tttgaccgtt ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt
    11461 tcatccagcg tatgattata ttttgaaatg cagccataca ttcaatttat tagatgctcg
    11521 cggtgcagta tccgtaacag aacgagcagg ttatctttcc cgtatccgca acatggctcg
    11581 tgctgtagcc aaaatctttg tggcagaacg cgaaaaatta ggattccctt tattgaataa
    11641 aaacacagca aatccagaaa acatgaagga ggctgaataa gatggcaaaa gacttattac
    11701 ttgaaatcgg gctggaagaa atgcctgctc atgttgtcac accaagcatg aaacaattag
    11761 aagaaaaaac agcaaaattt ttagatgaaa accaattgac ttatgatacg atcgaaacgt
    11821 tttctacacc gcgccgctta gcaatcaaag taacaaacat tccagaacgt caagcagaca
    11881 gtgaagaaga agtcaaagga ccagccaaaa aaattgcttt ggacgcagat ggaaactgga
    11941 cgaaagcagc tgaaggattt gtccgcggac aaggtctgac gacagatgct atcactttta
    12001 gagaattaaa aggcgtggaa tatgtctatg taacgaaata cactcacggc aaagaagcga
    12061 aagaagtatt gacaggttta gcagatgtca ttaaaagcct cactttccca gtaacgatgc
    12121 attgggcaga atacgatttt gaatatatcc gccctattca ctggatcgtg gcattgttag
    12181 atgacgaagt aatcccgttt gagatcttag atgtcaaaac aggcagagcc tctcgaggac
    12241 atcgtttctt aggcgaagat gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga
    12301 atgaacaatt cgttattgct tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa
    12361 atgaattagc cgaaaaaaat cactgggtta ttgatctaga cgaagattta ctagaagaag
    12421 tcaacaattt agtcgaatat ccaactgtct tttctggcga cttcgaagaa aaatatcttt
    12481 ctgttcctga agaagtacta gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac
    12541 ggaacgatca aggtatgctg cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa
    12601 aattggaaaa tgtggttcga ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg
    12661 aattctttta taatgaagac aagaaagctt caatcgacca atacgttgaa aaactaaaaa
    12721 ctgttacttt ccatgaaaaa atcggtagta tctatgagaa aatgcagcgg gtacaagtca
    12781 ttgcccgtat cattggcgaa caagtaggac tatctgagga agaattgatg gatctgcaac
    12841 gagctgctga aatctataaa tttgatctag taaccaacat ggtaggggaa tttcctgaac
    12901 tacaagggat catgggcgaa aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa
    12961 cagcgattcg tgagcattac atgcctatat ccagtgaagg agagctacct cagtctacag
    13021 taggttctgt tttagcgatt gcagataaat tagatagcat tttttctttc tttgcagttg
    13081 ggatgattcc aagcgggtca aacgatcctt atgccttacg tcgccaagca tacggtgtcg
    13141 ttcggatcat cgaatcgaaa aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta
    13201 gtgatgttat cagtaaagat acggatcgtt tcggcattgg tttatcagaa gggcaacaac
    13261 aggtgatcga ctttatcaaa ggccgtctgc gccaattatt gacaacgaag aatattcgtc
    13321 atgatgtcat cgaagctgtt ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag
    13381 cagcacaatt gttcaagcag catcttgcag atgaagactt caaaccatcg atggaagctt
    13441 tgacacgtgt cgtgaactta gcgaaaaaag cggagcttga aaaacaatca gaagtagacc
    13501 ctgaactgtt cgaaaatgaa gcggagaaag aactgcacaa agcagtagag gcaatgaaaa
    13561 aaagctttgc tgaccaaacg atcaacgaaa attatgatca gcttgtaagc cttcgcccac
    13621 tgatcgaaaa ttattttgat cagacgatgg tcatggcaga agaaccagca gtacgtaaga
    13681 accgattaac gcaattaagt caaattgctg ccatggcttt gtcgctagca agtttagatc
    13741 aaatcatcac aaaataattt ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DAPCMT010000070        13750 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPCMT010000070 DAPCMT010000000
VERSION     DAPCMT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.070"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(191..625)
                     /locus_tag="RD743_001706"
     CDS             complement(191..625)
                     /locus_tag="RD743_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0563519.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            773..1914
                     /locus_tag="RD743_001707"
                     /pseudo
     CDS             773..1914
                     /locus_tag="RD743_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2040..3119)
                     /locus_tag="RD743_001708"
     CDS             complement(2040..3119)
                     /locus_tag="RD743_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDU0563520.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3222..3662)
                     /locus_tag="RD743_001709"
     CDS             complement(3222..3662)
                     /locus_tag="RD743_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0563521.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3719..4129)
                     /locus_tag="RD743_001710"
     CDS             complement(3719..4129)
                     /locus_tag="RD743_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDU0563522.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4309..4743)
                     /locus_tag="RD743_001711"
     CDS             complement(4309..4743)
                     /locus_tag="RD743_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0563523.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4843..5691)
                     /locus_tag="RD743_001712"
     CDS             complement(4843..5691)
                     /locus_tag="RD743_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDU0563524.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6080..7846
                     /locus_tag="RD743_001713"
     CDS             6080..7846
                     /locus_tag="RD743_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDU0563525.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7948..9207
                     /locus_tag="RD743_001714"
     CDS             7948..9207
                     /locus_tag="RD743_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDU0563526.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9354..10655
                     /locus_tag="RD743_001715"
     CDS             9354..10655
                     /locus_tag="RD743_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0563527.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10658..11509
                     /locus_tag="RD743_001716"
     CDS             10658..11509
                     /locus_tag="RD743_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0563528.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11522..12337
                     /locus_tag="RD743_001717"
     CDS             11522..12337
                     /locus_tag="RD743_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDU0563529.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12488..13654
                     /locus_tag="RD743_001718"
     CDS             12488..13654
                     /locus_tag="RD743_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDU0563530.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         4701 a         2453 c         2544 g         4052 t
ORIGIN      
        1 ggtcaagtac ttctttttta aaaaaagtat tcatcataaa agccactact tgcggatctt
       61 catcataaaa ttctgtagca gtacttgaaa agatgttcca ataaatatct aatgctgcat
      121 attgaatttg tacctctttt ccagaaaaag actttctgcc attattagaa aaggagatag
      181 acaaaccgta ctatgctaaa aatgcattaa tctttttcat tttcgtatac aaataagagg
      241 cactatagtt tgtttgctga ctgaaactga taatcgaatt tgtctgacag ctgaacaaag
      301 aagtcaagat ctgataactg actgattcgt tgcagtatct tccaaataga cgataatagc
      361 agtaagccgg atcaattcct tcaaaattca actttaaaaa aggtttttgc ttatcaatat
      421 gcagttctgt tagattgaga cgctcaaagt cttgttctaa ttctttgatg tatagtaaaa
      481 aatttgattc agacatatgg aaatgatctg ctaaatattg tgtagtaata aagaaacgat
      541 gttcagatag gaaaagaaaa atatctaatt ttgtattgct ttttttcttc aagataaacg
      601 ttttaatcgc ttcttgagtt tgcatgaaag aggctccttt ttacttaaat tgttaaaact
      661 tgaaaatatt actgtttttt tcataaattt agacaaaaaa acagtaaaca tataggtaga
      721 atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag ttatgagata
      781 ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta taaatcagac
      841 agtccacaca gaggagacag aaacagccaa atggacagaa accaagtggg ccaacgtcaa
      901 tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa aattaagaaa
      961 aggacaaaca gcgaccatca aagcaaaaat tgactattcg ggagacttag cacctatact
     1021 acaatcagct gttacatttg agaatgttga tcccactttg tcgattggct atgacgagaa
     1081 cactttgact atccaacaca agcaagcgat atttaccttc accgttacat taaatcagga
     1141 cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga acgatcatca
     1201 tttaacgccg tatagtcagc ggcagacaat tgagcaagat tcagccatcg actcaggagg
     1261 agatcttgtt gaagaaccaa cagataaacc agaaaatgaa aataagccag aagtaccgcc
     1321 tacagaaaat ccagacggag aacagaaacc ggaaatagaa cctggagaag aacccgatac
     1381 agaaactcaa ccagagccag acaataaatc aaaacctgaa ataacccctg gagaaaaacc
     1441 agatgtaact ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc
     1501 agacacagat aatgaaactg aaaatccaga aaaaccagag gtagatccag aagaaaaacc
     1561 agatgtgact ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa
     1621 caaaaaaaca attcaagagg atggaaaaaa agagagtaaa aaatcaaatc tagcaattct
     1681 aaaaataaat gaagagcagc tgaataaaaa atcacgaata tttgatagcg cccaatcagc
     1741 tgaaacctta aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa aaataaacag
     1801 ctaccaaaat ccggagagtc acaaaacaaa gttatactgt ggagcggaat cattctatta
     1861 agtatagcaa caatgctttc tgcaaaaaga tttaaacaaa acagatctct ctaacatttg
     1921 aataatgtct gtgaacgcat ttttcaaata aaaagaaata tccagaaaat gaccccaaaa
     1981 taccagattg aaatctgata tttggggtcg ttcatacttt atctttcttc atatttattt
     2041 taagggatat ctgtttcttt tggaagatgg ttcgggatct ttttcacttt caatacatct
     2101 aaaaagaagg taaagatcgg aatgccgaca atcaagcccc aaacgccaaa gaaccgttcg
     2161 ctaacaagta agaccacaaa tgtataaaaa atcggcagtt ctgttttgct ggacatcaac
     2221 ttcggattca gcacataaga ctcaattaga tggacaatca aaatcgtaat caggatataa
     2281 acaacatctc ggatgcctcc ttgggaataa gctatgaaag atagaggaat acaagaaatg
     2341 ataacacctg ctactgggat cagacttaaa atgaaaatca tgatcgctaa actcagcagc
     2401 tgatgaaagc caaatacagc caaggcgatt gtagtgagca acgtatttaa aagagcgaca
     2461 acaaattgag cttctaatac caaaccaaat gtattgacaa acttatcggc aaaatagtaa
     2521 atatcttgaa agaaccaaga aaattcactt tttaaaaaca atcttgagaa tacaattgtt
     2581 tttttctttt caatcataaa aaagaaactt aagatgaatg acatagcaaa cgccactgcc
     2641 atttttccta tatcctgtaa atagccaagc gcaatcgaag cgccattttg aagctgttca
     2701 aaaaaatcgt ttcgctcaat ataagtatgg atatattgta aaacaggatc tgcatctttt
     2761 ggctgatgct cgtaaaaatt aataacggac tggatcatgt caaatgtttg atgaataagc
     2821 atcgggatat actttgtgaa tgccagatag ataaggtaaa cgatcagtgt ataaatcatg
     2881 atagctaaaa cacctgtagg gattcggaca aatcgctgaa tgatttgaac aagatgaatc
     2941 atcaaataag taaagataaa tgtgagcaaa atggttgtaa tcatacttct aactaagaaa
     3001 agaacaaaaa cgacaaataa taaaacagtg agacggcgta ttttttcatt cgccaaaaaa
     3061 cgctgataaa cggtcaaatc gacaacctcc aaataacaaa aaataaacac gttattcatt
     3121 atatgatgga atatgaaaat aagaaagagc aaaaagaaaa accgctaaat gacgttatct
     3181 tatgagttgg ttttcctcat aaaatcgttc atctagcagt ttcagccctt tttctggtct
     3241 tgatgaaaat tatctagcat ttttcgggtc agatgttgca gtgtatccaa ttcttcctgt
     3301 tcaagtggaa gataagactt gatcttttgt ggaatacctt ctgcttgttt ttgcaacgca
     3361 gcaccttgag cagtcagcgt aacatttacg attctttcat cacccgtttc acgctgacga
     3421 ttaatataat ttttttctgc taactttttt agtaagggag tcagcgtacc agaatccaga
     3481 cacaaatcat ttcctagctc tttcactgaa gtctttttct tttcccataa aactaacatc
     3541 acaagatact gggtataggt aagattcagc ggcttgagga ggggagtgta gtgtctaatg
     3601 atttctttag aggcggcata taaaggaaaa catacttgat taactaaata tagatcttcc
     3661 atttttccac ctccgctgtt ttactatagt gtatcatagc ggatttacac aaaaaagtct
     3721 attcactaac tgctttcaga gtaacttcta tatttccagc tgtagctttt gaatacggac
     3781 acactttatg cgctgcctct aataattctt gcgtttcttc cagagaaaga ccttcgatag
     3841 atccttcaat ctctacacct aagacaaagc tcgtttcact ttctgaataa agggaaactt
     3901 gtgcactgac agtagaagcc ccagagatat ttttttgctg tttgatcaat tctaatgcac
     3961 tgttaaaaca tgcactgaaa ccagctgcaa acaattgttc agggttggtt gcattttcga
     4021 cgcgtttacc aggagctgca atattcaatt cgaatgtacg atcgggatta tgcacttctc
     4081 cagagcgtcc gcctgtgttg atcattttcg tagaatacat ttttttcata aagttaccac
     4141 ctttaattat attttataca attgaatttt acacaatata attgtggaaa gcaaagaaat
     4201 ggattggcat aagataaata agagaaagca catgaaacag acgcgctttc tcgaatagca
     4261 aacatataca aaaaatatca gagacaaaaa ataatctcat cataaacatt acatatattt
     4321 ctcaaaaagt tgttttcttt cttcaaaaat tacttcacga tggacctgat gaagcatcca
     4381 taaataacca aaaggatctc tgaacattga gttagtcaca ccaaagtcca tcatttctgt
     4441 catttcttga atctctacac agccatttcg taatgcttta ttatgtgtgt cctcgatatc
     4501 agagacgatg atattgaacc agatcgtatt tggatgttca gcagtaggag cagtcaaacc
     4561 aagagacgtg ttttcatcca gcatatggaa tctcgtacca tacaaggtaa aaatgacttc
     4621 attattgcct ctagggtaat ctgttgcctc aatcagttgt atttcatcaa agattgtttg
     4681 atacagttct agtgctttca aactatcatt taatacaaaa tcgatttccg ttcctttatg
     4741 cataaagatc tcctctcctt ttcagtcgaa cagttactag attcttttat tataccagct
     4801 aaagaaaacg cattcaaaaa aatagcggat ttacttacca actcattcga ttggatgata
     4861 agtaaatccg cgtcccatta cttctgctac gctgctgaca atgacaaagg cacttggatc
     4921 gatttcttct atcaatggtt tcattcggga aaattcatga tcagtcacaa cgatcaataa
     4981 aacctctttt gactttcgat tatacccgcc ttttgcctct agcagggtaa ggccacgtcc
     5041 aatctcagca cttaagcgaa ggcggatgtc ttccaaataa tgttcactca tgaccataac
     5101 agatttcttt cgattgatac ctgtttcgat ataagtcatg acaattgaag tgattgcaat
     5161 agataaaata gcataaagaa actcttctat tccaaaaata aacagattaa gagaaacaac
     5221 gaatgcgtct gtaaaaaata agccaatcga agtattcaag tggaaatatt ttttaaaaat
     5281 gaggggaggg atcgtcgtac cgccgctaga agagttgttt ttatataaaa tcgcaactcc
     5341 taaagcaaaa atcgcacttc caaaaatgac agacaacaac cgatctgaag taatcattgt
     5401 ttctggaact attgcaatga ttaaaggaaa aaccaagctt ccgaaaagta ctttaaaaaa
     5461 gggtttttta cctaaaaata gaagagctag taccaacata acgatattca acactaaaat
     5521 aactatagct cggtcaaagc ctaaagcagc ttccagtaat attcctagac cgctgacacc
     5581 tccagcagca atatgatgag gccctaaaaa catattgata gcgactccta atatgaagag
     5641 ggcaaaaata atcttaatgt atgtccatat aaattgtctc gtttctttca tttattcact
     5701 tccttaattc attaataata ctcttatctt attataactt acttttcacc ttaagttaac
     5761 tttaattaat aaaaaaatta ggctagtcta aattattatg tttcattctt ctagaatata
     5821 cttctacgcc aaaaactgtt aaaaaacttt tactttagaa aattggagaa attgatcgct
     5881 taagataaat tcaggacact aaatagtaca ggaagtagac taaaactttt aaaagtttta
     5941 agtctatcta tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac tgttaccgat
     6001 aacatttttg aaagcgttgt cttttgcttc tctatcactt atagtgatga cgtcatatta
     6061 aaacacaagg aggaaatcaa tggatacagc tgcaatttat catcgaccgg aaagtgaata
     6121 tgcatttcta tatacgaata caagatttcg tattcggctt cgtactagga aaaatgatat
     6181 caaaaaagta tatgtgctat gtggagatcc atataccata acaactgaaa aatggtatca
     6241 aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt catgattatt gggaaattga
     6301 agtgacgagc gaaacaagtc gcttacagta tgcgttccat gttgttgggg aagataacac
     6361 ggactgtttt tacggtgatc aaggaatttt tccttatcga gaaaatgtga ttactgaacc
     6421 aaatttttat tttcgaatac cttattttca tcaaatcgat cgattcacta ttccagaatg
     6481 ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga tttgcaaacg gggacaaagc
     6541 aaatgacccc gaacacactt taccttgggg aagtaaagat ccagaccgag aagacttttt
     6601 cgggggagat ttgcaaggtg tgatggatca tttggattac ttgacggacc ttggcatcaa
     6661 tggcatctat ttttgtccca tatttaaagc cacatccaat cataaatacg atacgattga
     6721 ttattatgag gtggatcccg cttttggcga taaaaaactt ttaaaaaact tgattgatga
     6781 agcacataaa agagggatac gtatcatgtt cgatgctgta ttcaatcata tgggtgtcca
     6841 ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata aagactggtt
     6901 ccacatccgt tttttcccag tagattctta tcagatgact gacttaccag aaactgctga
     6961 aaatatccca tatgatactt ttgcttttac tccattcatg ccgaaactaa atacagcaaa
     7021 tccagaagtg caaaaatatt tgctggatat cgcaacttat tggataaaag aattcgatat
     7081 tgatggatgg cgtcttgatg ttgctaatga agtcgatcat catttttgga aaaaattcag
     7141 agaagcagtt acagaaatca aaccagacat ctatatcctt ggagagattt ggcattcctc
     7201 tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg aattatgcat ttacagattc
     7261 gattaaagat tattttgcaa aaaagaaaat cacagcttca caaatggtca gtggaatgaa
     7321 tcatcagcaa atgctttaca gagatcaggt gaacgaagga acattcaact tattagattc
     7381 tcatgatact gccaggatct tgaccctttg ccaaggtaac aaagaattga tgaagtctgt
     7441 gatggccttt atgtttcttc aaaaagggtc tccttgtatc tattacggga cagaaatagg
     7501 gatgactggt gaagatgacc cggattgccg caaatgtatg atatgggaga aggaggaaca
     7561 agatctagag ctattcggtt ttataaaaga acttgtttct ctaaggaaac agttgtctaa
     7621 gattatttct gaaggttcta ctcagtggct aatagtaaat gatcgtgaag ataagctcta
     7681 tttcacacgg aaactagagg gacagatcat ctatgtttac tttaatcagt caaaagaacc
     7741 ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt tgttagaaga
     7801 cgggaaagca gagatacgac aatacggatt tttgatcatg tgttgaaaac tgttaccggt
     7861 aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat tataataagg gtgtaacagc
     7921 acgttgaata caaaggagga aatgaagatg aaaaaaagaa gtgtgaaaca gttgggttta
     7981 gggttaatct ctttaggaat cttaggcgga ttagcggctt gtggcaatgg taactcttca
     8041 gcagaagggg aagccgatga aggaaaaacg ttaacagttt ccgttgattc tggatataaa
     8101 gattatataa acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa aatcaagcta
     8161 gtagaaaagg acatgttcga tcagttggaa tcattagcgt tagatgggcc tgctggaaag
     8221 ggacctgacg ttatgatggc agcatatgac cggatcggtg cgttaggaca gcaaggacat
     8281 ctggcggaag taaaactggg aaatgaagca gcttacgatg aaacagacaa agcccaagtg
     8341 acttatgatg gaaaaatcta cggagaacct gcagtaatcg agacccttgt tttgtactat
     8401 aacaaagatt tagtcgatac agcaccatca acgtttaaag atttggagaa tctatccaaa
     8461 gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata caggattttt agctaaatgg
     8521 actgactttt attattccta tggtttaatt gcaggttacg gtggctatgt atttggtgat
     8581 gatggcacaa atccttcaga tatcggtttg aataatgcag gtgctgtaga aggaatctct
     8641 tatgcaacag attggttcaa aaatgtttgg ccaaagggaa tgcaagacat aaaaagtgca
     8701 ggagacttcg ccagccagca attcatgtcc aataaaactg cagcaatcat tgacggtcca
     8761 tggcaagcgc aaacatacaa agaaaacaat atcaattatg gtgtagccaa aattcctact
     8821 ctaaataatg gacagcctta tcaaccgttt ggcggaggaa aaggatgggt agtcagcaat
     8881 tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa tcaggaaaat
     8941 caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa accaacaagc tagggaaaca
     9001 gcaaaggcta aaaacgatga attaacaaca gctgtgattg aacaatatga aacagctcaa
     9061 gcgatgccaa acatcccaga aatgggcgaa gtttggacag gtgcagaaaa cttgatgttc
     9121 gatgcagcat caggttcaaa aacaccaaaa gaatccgcag atgaagcagt aaaaacaatc
     9181 agtgaagcca tagaacaaaa atactgatta aaatgataac cgtccatttt tataaaaaat
     9241 ggaacaggtg cttcagataa aagtggcaag agcgagatgg aaaattatct tgctcttgtt
     9301 gccattatct taaaagcagt ttgcggttta gcaagtttag agggggcatt cagatgcagc
     9361 aagataaaac aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt attccaggga
     9421 tggggcagtt tgtaaatggc caagctttta aaggtatcgt ttttttcgcc atattcgtcc
     9481 tattcattgg tcagatgctt ctgggcggat ttcatgcatt ggtaggattg gttactttgg
     9541 gaagtatacc aatggaagat cattcattat ttattttgat tgaaggcact ttgcaattca
     9601 tcattacaat cattttcttg attttttacg gtttgaatct tttagacgct tatcgagtag
     9661 cgaagatgcg tcaagagggc aaaaaaatca atcggacgat gaaggaagtc atacataata
     9721 taggtgatga aggttttcct tatctattga cacttcccgc ttatcttctc atgattttta
     9781 cgatcatttt cccagtccta gttacattgt tcactgcatt tactaattat gacttcaatc
     9841 atattccacc agccagttta attgattggg taggggctaa aaactttttt agtatcttct
     9901 ttttaagctc atatcgtaat actttttttg cagtcttttc gtggaccgtg atttggactt
     9961 tatgtgcaac aactttgcaa atcacccttg gtattctcac agcggttgtt gctcaccaga
    10021 aatttattaa attcaaacgt attttcggta tcattttctt attaccatgg gcggtcccag
    10081 cttttattac tatcatgagt ttttcgaata tattcaacga tagcgtaggt gccatcaata
    10141 ctcaagtgat tcctttcata aaccagcttc cttttgtaga tatcagtgca atctcttgga
    10201 aaacggaccc gaactggacg aaagtagcaa ttatcttagt gcagggctgg ttaggatttc
    10261 cctatatcta tgtgatggtt acaggaatct tacaatcgat ttcagaagac ttatatgaag
    10321 cagcaaaaat cgacggagca aatgcatggc agcgcttttc aaacatcacg cttcctgctg
    10381 tcttcctagt ggcagcgcca acatttatta cacagtatac ggggaatttc aataatttct
    10441 ccatgatcta tctgtttaac aatggcggtc ccggaagcgt aggcggagga gctggttcta
    10501 ctgatattct tatttcctgg atctataaac ttacgactgg aacttctccg cagtattcta
    10561 tggcttcagc aatcacgttg attatctccg tcatcgttat ctgtgtgtca ttgctggtat
    10621 ttaaaaagac aaatgcgttc aatatggggg attaacaatg aaaaaatttc attcgagtgc
    10681 tcgattccaa aaacgacttt ctcacttttt tacttacttt tttcttttaa tactgtcagt
    10741 cattatcatc tatccgcttt tgatcacagc aagtactgcc ttcaagtcag gaaatattgc
    10801 agcttttagt ttagatcttt caagtgactg gactttagga aactttactc ggctattcag
    10861 tgaaacactt tacgggactt ggtataaaaa tacattgatc atttctgtcc taacaatgat
    10921 cgtacaagtg actgtagtaa ctttggcggg ctatacttat agtcgctatc gttttatcgg
    10981 ccgcaaatca agtttgatgt tctttttagt gattcagatg gtaccgacta tggcagcatt
    11041 gactgccttt tatgtcatgg ctttcttatt agaagcgctc gataagtact ggttcttgac
    11101 aatgatctat ataggcggtg gaattccaat gaatacttgg ctgatgaaag gatactttga
    11161 cactgtacca atagatttag atgaatctgc taaacttgat ggggcagggc atttcaggat
    11221 ttttttccag attgtattac cgcttgtcaa accgatgctc gccgttcaag cattatgggc
    11281 ttttatggga ccttttgggg atttcatgtt agctaagttc ctgcttcgaa cacctgaaaa
    11341 tcagactgtt gctgtcggct tacaaacatt tatttctgat gtgacaaacc aaagagtctc
    11401 tctttttgct gctggggcaa ttttgattgc actaccgatt tgtctattat tcttcttttt
    11461 acaaaagaac tttgttaccg gcctgacatc tggcggaaca aaaggatagg agcagagtat
    11521 catgcaaaca tttccgttaa actatttttc cgcattacgt acgccaactc aagtgtttgc
    11581 gggacgcaaa ctattatcat ggcctaaatt tttcttgata tttgtatttt tagtttcctt
    11641 gatggtcatg cctgtgactc ttttttatgc caatcagatc caagcgattc cattagaaca
    11701 atttctatcc gttcattcgc tgatcgatga acaaggaacc caaaaatttt ctgaacttga
    11761 attatctgaa actgggttac aagcatcgca acaaacgata gctgtgaccc ctgaaatttt
    11821 ggtaggtgtg tctttatctg agaaacaaca atccgatcat ggaactttta ttgattttga
    11881 gaaggaacaa tgggtgatcc aacaaaagga taaatctggt atacgccgct atacgatgaa
    11941 ttatagccca tctttccaac cagattctgt acgtacgccc gaagatttcc aacgattttt
    12001 agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt ttatcttata gtttaagtct
    12061 tggtcttgtc ctatttgtca tgacgagtct cattttattc ggggcttcct ttttcttgtg
    12121 gatgacaaga aaaagccaac tttcatccat tcatacattc aaagagagtg caaatttgat
    12181 gctgaatgtt atgggaatag gttccatggc agcagcagtt gtagggttta tccactttga
    12241 ctttatcttg atgctaagtg tccaaacaac gataactgtc ttgctgcttt tatgggtttt
    12301 tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa gtttaagcga tttatgcgac
    12361 aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa aaatttctga aaaaattaaa
    12421 cttcttagtt gattcttttc ttttttagta ttaacgtaat agggtagttt ttttaaggag
    12481 gaaaatcatg ttagagaaaa ctttatacaa tcaattattt tcacgttctt tctcgctccc
    12541 tgtcgaagta aattattggg acggtacaac gaaacaatac ggagatacgg acactcctcc
    12601 ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa gagattacaa gtaacgcttc
    12661 tttggcacta ggagaagcct atatggatca tcgaatcgag atcgagggaa gtatccaagc
    12721 attgatcaac gatgcttatc agcaagcaaa cagcttttta cgaggaaaag attatctaaa
    12781 atggctgcca aagaaagaaa aacatacgaa acaaaaaaac aaagaagata tccatgcgca
    12841 ttatgactta ggtaacgact tttataaact ttggctagat ccaactttga cttattcttg
    12901 tgcttatttc aatacacctg aagatacgtt ggaacaagca caaatcaata aagtccatca
    12961 tatcttagac aaactcttta tcaaagaagg cgaaacgttg cttgatatcg gttgtggctg
    13021 gggaacattg atgttcactg ctaccaaaga gtataatgtc aaggcaactg ggatcacact
    13081 aagtgaagaa caatacgatt tgattaccaa aagaataaaa gaagaacatt tagaagataa
    13141 atgccgtgta ttattaatgg attaccgcga actaaaaggc gaaacgtttg atcatatcac
    13201 aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt gaaggttatt tcaaagtggt
    13261 aaaagattta ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc gacaacaagg
    13321 cggtgcgaca aatgcttgga tcaataaata tattttccct ggcggttatg tccctggtgt
    13381 tactgaatta gttgataata ttacaaaaaa cgatttgcaa ctgattgatt tggaaagctt
    13441 acgtcgtgac taccaattga cattagaaca ttggacaaag aattttcatc aagtccaaga
    13501 tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg tgggatctat atttacaagc
    13561 ttgtgcagct tcatttgaat caagtaatat cgatgtgatt caatatttgc ttgttcaacc
    13621 tggaaacaat gacatcccaa tgcatcgtgc ataattgagg caaaagtact gtttaaagac
    13681 tatttaaaaa gaagctaagt tttatgatgc tccagaagtg aacttttttg tctaacttct
    13741 aggattgtct
//
LOCUS       DAPCMT010000071        13729 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPCMT010000071 DAPCMT010000000
VERSION     DAPCMT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.071"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            38..1450
                     /locus_tag="RD743_001719"
     CDS             38..1450
                     /locus_tag="RD743_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDU0563531.1"
                     /translation="MSQLLIATPFHVLASENSTYKIAKERTETTEMSNSDVTAESSEL
                     LTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESST
                     ISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQS
                     QPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQL
                     AQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLT
                     KGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEV
                     KGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFH
                     YSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEIC
                     KDSSLSQSMLVDPKTLLFGD"
     gene            1799..2431
                     /locus_tag="RD743_001720"
     CDS             1799..2431
                     /locus_tag="RD743_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDU0563532.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3019..4458
                     /locus_tag="RD743_001721"
     CDS             3019..4458
                     /locus_tag="RD743_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0563533.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4753..4971
                     /locus_tag="RD743_001722"
                     /pseudo
     CDS             4753..4971
                     /locus_tag="RD743_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5220..5435
                     /locus_tag="RD743_001723"
     CDS             5220..5435
                     /locus_tag="RD743_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563534.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5652..5908)
                     /locus_tag="RD743_001724"
                     /pseudo
     CDS             complement(5652..5908)
                     /locus_tag="RD743_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6095..7006)
                     /locus_tag="RD743_001725"
     CDS             complement(6095..7006)
                     /locus_tag="RD743_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0563535.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7025..8029)
                     /locus_tag="RD743_001726"
     CDS             complement(7025..8029)
                     /locus_tag="RD743_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDU0563536.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8026..10149)
                     /locus_tag="RD743_001727"
     CDS             complement(8026..10149)
                     /locus_tag="RD743_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDU0563537.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10154..12601)
                     /locus_tag="RD743_001728"
     CDS             complement(10154..12601)
                     /locus_tag="RD743_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0563538.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12588..12977)
                     /locus_tag="RD743_001729"
     CDS             complement(12588..12977)
                     /locus_tag="RD743_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0563539.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13037..13540)
                     /locus_tag="RD743_001730"
     CDS             complement(13037..13540)
                     /locus_tag="RD743_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDU0563540.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13553..13714)
                     /locus_tag="RD743_001731"
     CDS             complement(13553..13714)
                     /locus_tag="RD743_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563541.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4220 a         2710 c         2509 g         4290 t
ORIGIN      
        1 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
       61 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
      121 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
      181 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
      241 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
      301 aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc
      361 ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca
      421 tatagaatct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac
      481 accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga
      541 agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc
      601 tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat
      661 cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg
      721 gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa
      781 tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt
      841 tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg
      901 agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta
      961 tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac
     1021 caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat
     1081 tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact
     1141 tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa
     1201 agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac
     1261 cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg
     1321 acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga
     1381 aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt
     1441 cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga
     1501 cctttgatca ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata
     1561 aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag
     1621 caggtttcac tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca
     1681 gtcttgtatt gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct
     1741 gatttattaa aagttaattg aaattatatg gtacttcttt cttgggaggg taggtgcagt
     1801 gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa
     1861 atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc
     1921 aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt
     1981 gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg
     2041 aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg
     2101 acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga
     2161 aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa
     2221 aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt
     2281 attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt
     2341 tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt
     2401 taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaaagag
     2461 gtactattta ggatcgtatg attctattaa gagtgcagct caagctagaa aagatgctga
     2521 aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat tattgtggat
     2581 aataattaac agtgatatat aaacatctat aatatattat tcatactatt gatatttagt
     2641 ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg gaaaacaaat
     2701 aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat ttgcttacgt
     2761 ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt tagaaattgc
     2821 tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa taaagcatct
     2881 ccaacttaca tggttacatg aggatatcaa atcgaaatta ctcaagcttc ctatggctag
     2941 agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct acacgatcat
     3001 tagaaaggaa atcctattat gtatagttat caagaagatg caaaagtcca aaaaaatcgc
     3061 tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga tggcagtatt
     3121 gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa tcaagcggaa
     3181 tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt tggtaaaatt
     3241 ggcgatagcc gtgggaaaat caaagtattt cggattggta cgctgatctt tgtcttggga
     3301 tcgctgttgt gtgggttcaa tcattcgcta ggtattttat tgttcgcacg ggtggtacaa
     3361 gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga agtctttccc
     3421 atacatgaac gtggtcgggc attgggagca ataggtgcgt ttgtttcatt aggctcgatt
     3481 gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta cattttttgg
     3541 atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt accaaaagat
     3601 atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc ggctacgatt
     3661 atcagctttt ttggagcaat ctttttgggg caagaaagtg gctttttcca tccagtcccc
     3721 cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt tgaaaagaaa
     3781 gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac catgagcttg
     3841 attacagcct tgttgatttt ttcttccaac ttctttgtca atgtagtgat tcctttttat
     3901 ctgcaaaatg ctcgcgggtt gagtcctagc tatgctgggc tgttgatgat ggtctttcct
     3961 ttgatgatgg tcgtcggggc gcctttaagc ggctacctga ccgaccgggt tggcccggag
     4021 attttggtat tagtaggctt gggcttattg tcggtaaccc aggtcatgta tatgtttttg
     4081 tcagtggact ctcctttgtg gctatacatt gtcgctactg ccatcatggg tgccggcaat
     4141 gccttgttcc agtcccccaa taatacgatg gttatgagct cggtcactat cgaaaacttg
     4201 ggactggcag gctcgatgaa ttcctttgcc cgaaatttgg ggatggtcag cgggatttcg
     4261 ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccagca agtaactaac
     4321 tatatcgcaa atcggccgga tgtcttttta tatggaatga agattacctt tttaggcagc
     4381 tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa acaaggaggt
     4441 attcagaaaa atgtctgaaa aaaccactgt tgaagaattt ttggaggcct gcgtatgggt
     4501 gagtgggatt tccatggatg atggaagatg aagtgaactt ctaaaagccg aaaaactcaa
     4561 gtattatctt caaaatacta gtgctcaatg agatgcttga tttgatcaca agaagactgc
     4621 gaaagccgct ggcaaccgca agctaggtat cccaccgatg gactacgttt tagaggctac
     4681 tggttatgaa tacggagcca acgctctcat ctgcataaga atacatcacc cagattatgt
     4741 aaagattttc gatacttctg tcaaaaaatc attaagatag ataaatgatt tccgtctagt
     4801 caaattggtt cagaatgtag acacacagat ggtaagaagt cactcacaat tcgagaatgg
     4861 acttgtccta tttgccgtat acatcataat cgaaacgtca acgatagtat caatattttg
     4921 accaagggct atgaatacaa gcattgcctt agaaaacatc aagaacctta ggaacaacgg
     4981 ggatagcttg gttaataaga gataccgctg tttaggcttg caagtatact cttttatcaa
     5041 gaattccgtg gctttagtca tgagaggctt aatactatga aaattttctt tagcattttt
     5101 gatttaggtt ccttttctaa ttataataag atgaccacta cagtaaccta ctgaaatata
     5161 taaacagcca ctgtagtacc attttcaaag tagtctatga gaggagaatc ttcattctta
     5221 tgaacgctgg aattgcggat atgaatttaa taaaaaaaac ccttaatgat tttacttcca
     5281 attcaatatc aaaaggtact ggtatcaatt taagcacaat aaaaaagctg aaaagtggtg
     5341 aaagatcagt ggaaaagctg aatcttttag atgctataaa aataacggaa tttgctatga
     5401 aaaatggaaa agctgagatt gaaatatgga gataattatc gtctagtgaa ttacttgcca
     5461 cttagctgaa tttaacgatc cttggtgctt gaagggggag attcttggga atagagcata
     5521 cttgcaagcc tatgtcttta ctaacaacga tatctcttat taaccaagct atccccgtag
     5581 ttctaaggct tttactttta tctaagcaat aatcgtattc aaaggaaatt tttgaccctt
     5641 agaaagtctc attttatcag acgtgagctt aggcattgct gaattttgtt aattcggttt
     5701 taggttgtgc tttgagcata atatgaaagt attctttatc acgatttcat tcatctaagg
     5761 taatgtgatg tgattctgaa attctttcaa aggcagtttt tgcataagct gacattacat
     5821 catcaatgac atgtttacaa tgtttcacta ctaaaataag ggggtaacca agaagaaaaa
     5881 ctgaatgatt atttgtatct aattccatgg tatatcaacc atttcctata tagactgatt
     5941 atgacatgga ttaaaataaa aaggcactat gtcttaattg tcggttttcg agttaccgaa
     6001 tggcattcat cacccaccta tagaggatgg gcgacttctg cctgaaataa gttacactac
     6061 ttataacttc taataaaatt agattgagtc attattattt aacatcaacg attttccagt
     6121 ttccattttt ttgtaatact aatttgtact gaaagtagct agtggttttt gatacattat
     6181 caaggtattt tatctttgta ctcactagaa aattttttcc gttttgtaga taaactggat
     6241 tctctaactc cgaaaacttt aagtcaatat tgattggtcg catcgcacca ttttcaacat
     6301 aatactccaa ttctttttca gaagctgatg gataaagttt aaaaaatgtt tctaaaaaat
     6361 cggtgatttc ttccgttgtt ttggcatcaa tttctgaatc gttttctact tgttttgctt
     6421 catattttgc cttgtctggc tttccaatca ttgttggcaa actagttact aaatgattcc
     6481 tgttttcatc ttcaaagatt gtcactcgat atgcagaaga aattgattgc gtcttttttc
     6541 cctctgtaat atgttgctcc actaaaaatg aaacgacaaa ttcttccgag tgtttttcaa
     6601 catctagaat ttttacagat tgaacttcgg aactcgtggg aatatctgct cgaaccatat
     6661 cttggattaa agcgagaccg ttttctgtaa ggtactgttc gagattactc attctctttt
     6721 caattgcttc tttgttattt ttccatgaaa aatattcttt cgcaaaatcc tctgtaaaac
     6781 tttcaacgcc agaagtattc actaccttct cttttataac cactttttca tgagttgtat
     6841 gttgatcaat ggcggtgaaa tttttataaa cgccaaaagc aagactacaa gacaataaaa
     6901 gccaaagggc aagcaccatt tttcgatgct tacccacgga tacaactcgt tcttttttct
     6961 tttttgggct cttttccttt tgattccgtt caatttttat cttcattctt tttcctcttt
     7021 tctcttattt gtatcggcca gctccgataa gatgctgccg ccaatagttt tccgttagat
     7081 tcgtccaccc cactggatct cctgcatgaa acatgcgatt ctcccctgcg taaatgccca
     7141 catgggtcac ataagtaccg gcatcatagg ttgaatgaaa gaaaactaaa tcacctggtt
     7201 tggcatcttt tagatcaata tgagtcatgg catcatattg atcctgagcc acccgaggaa
     7261 gtttcaggtc aactttggca taacaccatt gggtcaagcc actacaatca aaccccgttt
     7321 ctggacttga gccaccaaac acataggccg ttccttcata cttaaatgcc tcatcaaaaa
     7381 tttcttggac tattttgtca tcaaactgag ttgtagttag gtattgcttc actaataaac
     7441 agtaaaacat attgccataa ttataccgcc acccaccatt ttccttaatc gcgattgggt
     7501 tggggtaatc gactttttga ccacctgatt tttcctttga aaaagattca gctaaagcaa
     7561 aactatgctt cttcccgttt ttcccgacat gatctagata cccgccacca tagttatagg
     7621 cctgaataat actatcttga tcacacccta gttctttgga acgcttaacc aaagacgcaa
     7681 aatagagaca gccttgtttg atggatgcct cggtacttaa tgaattaggt ggtaatccgg
     7741 ctgactcaga actttgcata acgtcttcca ttgtgccacc agattccact tgaataatcg
     7801 ccaaaagaat tggaatatac tctgttatat ccgattcttt ggcatacttt tccaccattg
     7861 cacggtgttt caatacttct tctgagacac tagttccgcc aggagaagtg ccagaactac
     7921 catttccctc actgtcttca ccaaacaaca aacccacaca catcaaaata cccataaaag
     7981 agatggtcag aaaagttaat gaaataacac ccactttctt caagttcatt ttttctcacc
     8041 tgaccttgat tttggacgat ggttcattct tttttgtact ttttgattgg tctgctttgg
     8101 cagagtggac ctctgtttca cttgttggaa agaatttttc ttacttgctg attggctttt
     8161 cccttttgaa atgatttctt gactagttag ttttcgtttg atttcgggat tcagatttgc
     8221 tctcacaatc cgctttggtg cttctttttt cagcatcata tctttctgag gttgagctgc
     8281 tttatctatt ggaatattcg cccttctttt tccctgacga tttgttgctt cactcagtat
     8341 cttgcgtttt tcgtccattt tcgcatttct tcgttgtcgt tgtttttctc gcaaatccac
     8401 tcgatccgct ttttcttgga acagtccgcg cttgaattct tctggtgctt ttttcgactt
     8461 ttcgattcct ttatggagat tgtatttcaa attcgttggc gtattcctta cctgatcctt
     8521 tacttgttta gtcttatctt tcaccaaagc ttgggtatcc aaaactttgc cgatttttct
     8581 cccagccaaa ttcattcggg attgtttctt attatttgaa actggattct ccttagttaa
     8641 gtcagatagt atttcgtgat cgttcggtaa tcgtgaattc tttcgtagag aagaaccgga
     8701 tggcttaaat ttggattttg attgttcctt tgccaactta tttccgactg ctgcaccacc
     8761 accaaaagcc aaagctcgtc ccacatttcg attgagttgc cgcaattttc gattcattaa
     8821 catttgcggt tttcgcatga cgcgtcttcc cattgactgg ctatcattac tctggaggtt
     8881 gaacatactc ataatgtcgc ccaatttgaa atagataccg gcaaaggtca caatttgcag
     8941 aaaagcaacc atgaaaaatg gataggttgc cgaaatattg aaaaacatcg tggagatgct
     9001 aaatgcagtc gtaatcacca aagtaacacc cgctcgcatc ataatcgtat tgaataaacg
     9061 aataatcgcc ttctttccta aactttcata agtcggcaac atggacaata agaaactaat
     9121 tggcagaaac atcgcaaaga taatgaagag aatttgcgag aacaacataa tccctgttag
     9181 aagaaaaaca aagaaggaaa tgccaatatt gaacaatacc aaaaagacca cggtccctaa
     9241 acggttcatg gtcttggtaa tactgagatt cgcattgtca ttgtcttcaa tctctgcctt
     9301 gaccgcttct tcccgatctt tgcctttgtt ttcatctgga ctcacagata gaatcttgtt
     9361 gacccggtct tcaccaattt cttctatgtt ggaatcatca aattgcagta gtaaccaagg
     9421 ttgttggact tgaatcgaaa acaaactatc ccgaatcaaa tccacactat cttttccttg
     9481 gctttctgaa ttgggtacaa cgatctcggt tccaagggta agggccgctt cactaacatc
     9541 tgagctaaag tcattgattt ttgtgatata ggtaggcgca taagcaatga acgaacccga
     9601 tagtagaaaa atcatcacaa aattaatcac ggctcgaact gctttcgtgg tttctcgttt
     9661 taataatccg gtatacgcca catacacccc cagagctaaa atcatcaata atagaaaccc
     9721 aaaataaaat ccggaggtct ggagtccatt ttcagtaata ccagccaatg tttggatatt
     9781 ctttccaata ctttcagccg tatccgaaat gaaatctaat ttgtaagcct cttgaaccac
     9841 ataacccgtg gcgttcgaca aatacaaact caccgtccaa atgaaattag taatggcata
     9901 aagaccatac atcacgcttt tccctaagcc atcaccccaa ttccaaggca accaatccca
     9961 agagctatcc acaaagaaat ctagttgata gttgttcagt gaatatttgg aatatagatt
    10021 tcctgcttca actgtatcat caactagccc tgtcgcctcg gctaccgtac caactaaact
    10081 caatagaact atcagagtga gtatgctaat aaaaacgatt cccacaatcc gaaaaagttt
    10141 ctttttcatc ccctcactcc attctctctt gaacaggtgg acgggtatca aaggcatgga
    10201 agagttcttc aaagactgga tgtacctgaa ccacgcctac ccgaccatac aaatcctgca
    10261 tcaagcattg gccattttcc aaatctcgta ggcgtttttg attgctttca tcttctcgat
    10321 ccaagccaaa gaattccaag gttttcttga tttcatgaat atcggtactg cgaaacgcaa
    10381 acttcaaccc gatattattc ttcaagcgtt catccagtaa atcatcacta ttttgcgtga
    10441 caaagtaaac cccggcattc atagcccgcc cagcacgaac gagcttattc gataatgttt
    10501 tcccttgcgc tacttgaaga aaactccacg cctcatctaa gtctacaatt ttgaagattt
    10561 ctcgattact atggataaag tccaaggcaa aagtggaaat gacgattagc atggaaacac
    10621 taagcaattc catggtggta tattcttcaa aagtggtttc tgcatctggt aaaaccaaat
    10681 ccgctacttg aatgatattt aactgcctat ctaaactaat cgactgggta atcgaaccat
    10741 ctgagaaaag caaatgggca aaatcataat cggtaaagga ctcgatgtga tccgcgatat
    10801 tttccgcaac tggtgagcct tcttttcgaa gctcattaat tacttgtaat aatccccgtt
    10861 gattgctttg ggtaaccgtc cgaatggcct tacgcaacac tggaaacttc tccccatctc
    10921 gactagaaat tcctgttaaa aaggtcagaa tatcaattgc tagactttca gaatccttct
    10981 ttttctccat aatcacataa ggatcaagta atccttgatt ttttggctta ctagttaagt
    11041 tgagaatatt gatttcatcc gcaatttctg gcaaggtttc tttccagcca ccccgttccc
    11101 cttttggatc gacaatcaca gcttgtccac caaaaaggac ggcgtaataa actaagagat
    11161 tgttactaaa ggactttccg ccaccgagtg aaccgaggaa tgccgctgct agcgcattgg
    11221 taactgaatc cttgacccct tgtgaggcca atgccggttt caggtaaaca ttgcgccccg
    11281 tatctacgtt ataaccaaag taaatgcctt ccaactcccc taacatctga gtggcgccaa
    11341 aaccaagtcc ggcgagaaaa tcagacgtaa catactggat ataatcattc atgtatcgct
    11401 tactagaagg taagaattct tcatgtagcc ccatcatatc gccaaaaggc cggactagtt
    11461 ttacattggt atcatcataa aaatctagca cttcatcaca ccggcgcttt agttcatcta
    11521 ctgattcggc acttactcga accacatagc tcagtttgta catggattct ttggactgat
    11581 ccaaggtagt ttccaattca tctacggaat ccaatgcatc taagacattg gaatttgttt
    11641 cattgtcaga ttgataggca tggttgtcca agtcttttaa ttcctttttc ttattgcgaa
    11701 cggtagcaag tgcctttttg ttcgtcacaa tctccacatt catactagtg tcaattggga
    11761 atgtaaattg ttgttgctgg taatagaaaa gttctgagga gggaaactcc atttccccca
    11821 caattgtatt aattgttaag tacgctacat atgattcatg attctcatgt tccatgcata
    11881 agtaccgggg cttttcttca atcaaacaac gactgggacg taataagtcg taacgtttca
    11941 ccaaagtttc cgacttcagc tttttctttg gtagctgaaa ttcatattct tcaaatggtg
    12001 tacccttttc gccatagata tgttcaatca aatacatcag atcactcggt gtgactcgtc
    12061 tgactttaaa tcttcgagcc aatttacttt ccattagttt ttcaagcttg gtatatcggc
    12121 gaatttcttc attggataag gaaacaaaat cgcccatcaa atgatgattc actccataga
    12181 caaattcttg aaagccggag aagaaagatt tcttgagatt ctttagattg acctcttcat
    12241 ctgtcgcaat cagtttgaag ccaataaaaa agcgataatc aatctgacta tcgccaatca
    12301 tggatactaa tgcctctgtc tgtaaatcaa ttcgttgttt cgctacttct gttaatcgcc
    12361 cggtaatttc cttttttgat cgttcttgcg tggcacgaat actactttct gtggcaattt
    12421 gtaaggcatg aatcttgcct tcacgatttt gcgcaatcaa ttgacgaaag ttatcgtgga
    12481 cttgatattt ctgttcaggt gacaaaaagg agtaattgta aggtattaac tcatagtagg
    12541 caaaacattc tccctcttga ttaaagacaa gattattttc aatatactta atcgggtaca
    12601 tagttcacac tccttaccag agtaatgggt tcgtcccatc gtttcttctg ttctttgact
    12661 ttctttcctg cgaaagtgac cttaaatcgt aagtgatagc taataaaaga tcgtaaaaat
    12721 cccaatggtt tcttaccgtc aaacgttttt tgcgacatga accaagtaac tgccaccggt
    12781 atacctaagt attttaaaaa ggccccctct atcatagata gtggaggcac atgggcaaat
    12841 acgatgacaa acagcaagga taaaacaaac caagccatct ggttaaaagt cacaggaaaa
    12901 ggcagttgaa agtcattaat tgcataaatg accttttcaa ctgcccaaat actggtgtag
    12961 ctttttattt tcttcatcaa atcattcctt taaaaagaag gatagtttag aaatcaacta
    13021 cccttcaata attttgttaa tatggatact caaaaacacc tcgactcgtt accaaataat
    13081 tgcctccaat ctccaagtct ctaccacacg catcaaaatc aatatacgtc ttcaagtgtt
    13141 ctggtacttc tccaaaatca ccagcttcta caatcaagaa atatgctaca tcactcatat
    13201 catcgcaatc tggataatag ataatatcat ccacatgctc taccatctcc tcaaaagaat
    13261 tgaagaaagc atgttgaatc tcagaagcaa cttcaccaac tggcgttcct tcaagttcct
    13321 ccgctaagcc acacaaccga ttaatttcct caattggcgt atactcatca atctcaaagg
    13381 gaagttcata atcatgaatt gcatattcct cgtattcgtc atttaaccca attcgttctt
    13441 tcacttcatc aaaatcgaca ggtggggtaa accacgcccc caccaattca ccttcgttat
    13501 atttcccgag gttcgcaata taaacacgca tctgttccat cgtgcttccc ccttacgcac
    13561 cgacaattcg gttaaacaaa ctgagtaaga catccttcac cccagaagca ttaaagacca
    13621 atccgaccgc aatcaaagca atcaccaaaa agccaatcag tttggaaaac tcccgcttaa
    13681 atcctaaaaa caaaccaatg accacaattg ccagtaagac taaggattg
//
LOCUS       DAPCMT010000072        13703 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPCMT010000072 DAPCMT010000000
VERSION     DAPCMT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.072"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            361..630
                     /gene="rpsN"
                     /locus_tag="RD743_001732"
     CDS             361..630
                     /gene="rpsN"
                     /locus_tag="RD743_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDU0563542.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            645..824
                     /gene="rpmF"
                     /locus_tag="RD743_001733"
     CDS             645..824
                     /gene="rpmF"
                     /locus_tag="RD743_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDU0563543.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            856..1233
                     /locus_tag="RD743_001734"
     CDS             856..1233
                     /locus_tag="RD743_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563544.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1254..1403
                     /gene="rpmG"
                     /locus_tag="RD743_001735"
     CDS             1254..1403
                     /gene="rpmG"
                     /locus_tag="RD743_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDU0563545.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1427..2410
                     /locus_tag="RD743_001736"
     CDS             1427..2410
                     /locus_tag="RD743_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDU0563546.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2440..2562
                     /locus_tag="RD743_001737"
     CDS             2440..2562
                     /locus_tag="RD743_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDU0563547.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2580..4103
                     /locus_tag="RD743_001738"
     CDS             2580..4103
                     /locus_tag="RD743_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDU0563548.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4127..4366
                     /locus_tag="RD743_001739"
     CDS             4127..4366
                     /locus_tag="RD743_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0563549.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4559..5020)
                     /locus_tag="RD743_001740"
     CDS             complement(4559..5020)
                     /locus_tag="RD743_001740"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0563550.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5215..5433)
                     /locus_tag="RD743_001741"
     CDS             complement(5215..5433)
                     /locus_tag="RD743_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0563551.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5551..7167
                     /locus_tag="RD743_001742"
     CDS             5551..7167
                     /locus_tag="RD743_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDU0563552.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7408..8748)
                     /locus_tag="RD743_001743"
     CDS             complement(7408..8748)
                     /locus_tag="RD743_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDU0563553.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(8907..9347)
                     /locus_tag="RD743_001744"
     CDS             complement(8907..9347)
                     /locus_tag="RD743_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDU0563554.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            9507..10013
                     /locus_tag="RD743_001745"
     CDS             9507..10013
                     /locus_tag="RD743_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDU0563555.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            10181..12094
                     /locus_tag="RD743_001746"
     CDS             10181..12094
                     /locus_tag="RD743_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDU0563556.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            12099..13100
                     /locus_tag="RD743_001747"
     CDS             12099..13100
                     /locus_tag="RD743_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0563557.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(13302..13508)
                     /locus_tag="RD743_001748"
     CDS             complement(13302..13508)
                     /locus_tag="RD743_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDU0563558.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         4785 a         2124 c         2772 g         4022 t
ORIGIN      
        1 aaagttttaa aaaattggcg ataaaccagt taaacataaa agattgaata aaaagttata
       61 gtttctttca tattgccgtt ttaagtagag gaaaagctac ttaaaacggt atttgtgttt
      121 gattggacta ataaaagtga attcttattt ttcagagcct aaactgcata ttcttttaaa
      181 tgatttgatt atctctatgt accgataaat tcgttttcaa atagttatct attcatatga
      241 gttttatttc atgttattta ttgttgacaa gagcaactct tgttggtaaa gtatcagtgt
      301 caaaacgtaa cgattacgat ttgaggtgtc taaatcaaaa taaaaggagt ataaaggtat
      361 atggcaaaaa agtcgaaaat tgctaaagca aagaaacaaa tggcgatgat tgaaaagtat
      421 gcagataaac gtcaggaatt aaaagccgca ggagatcgca ccgctctcgc taaattgcca
      481 agagattcaa atccgaatcg tctgaggctt cgtgatcaga cggatggtcg tccaagaggg
      541 tatatgcgaa agttcggtat gtcccgtatt aagtttagag aacttgccca tcaagggttg
      601 attcctggag taaaaaaagc aagttggtaa aaggaggaaa tatcatggca gtaccagcaa
      661 gaaaaacatc taaggcgaag aaacggttaa gaagaactca tcaaactgta gtaaaaccag
      721 aaatttcatt tgacgaagcg aatggtgatt atagacgtag ccatcatgta tctttaaaag
      781 ggtactataa tggaaagaaa gtaattaaag gagcttcaaa atagtttaat atataacttt
      841 taggagggaa tcaaaatgga aattatttac ccacctttag ttgaagaagg attaaagtat
      901 tatcttgaaa cgacccagca atcgctagat aaatgcactt tttatcgttc gatggtagag
      961 agaggaatta ttactgaaac gggtttaccg acccaacagg caattgaaaa tggattagtc
     1021 aaagattact atgaggacca aggcttatca tttgatgatt ttttaagaat ctatccaatt
     1081 tttgaagagt atgacgagga gttatttcaa tgtatcgatg gttattggga gatacctgtt
     1141 gatatgaaag aaaatttagt ttcacaattg gaatctggcg agttaacttt tgaagatgca
     1201 caacaaattc aagcatatct tgaagatcga taagtattga aggaggaaga tttatgcgtc
     1261 aaaatattat tttggaatgt gtcgaaactg gagaacgtct gtatctcacc agcaaaaata
     1321 aacggaataa ccctgaacgg ttagaattaa aaaagtattc gccaaaattg cgcagacggg
     1381 caatttttaa agaagtgaaa tagctaagtg gtaaaaaggt gacaaaatgg gcataccagt
     1441 tacaatcgtt tctgggtttt taggggctgg aaaaacaacg ctaatcaatc aagccttgca
     1501 agtctcaccc taccccaggg aagaaattat tataattgaa aatgagtttg gtgaagttgg
     1561 gattgatcac aaattattag ttcatagcga ggaacgggtg ttacaactaa ataatggatg
     1621 tatgtgttgc agtcttcgca gtgatttatt agcaatattg atttctttac tggaaacaat
     1681 agaagaacat caacaaccaa tttcacaaat catcattgaa actacaggta tagcagatcc
     1741 ccaaccaatc attcaaacac ttttaacagc gcctcaacta agaggaagag tatatatcga
     1801 tagtttattg actgttattg atagtgacag ttttgaactt attgaaagag aaccacaagc
     1861 ccttaagcaa cttgtcatgg cagatcgcat ttttgtgtct agaaaacaaa acttgaattt
     1921 atctgcacta aaaaaattac agagagaaat caaaagtatt aaccctttat ccgatattcg
     1981 aatgttcaca catagtgaaa cgattcaaaa aaaggatttt tataatttga aaaaatttaa
     2041 tcatccctta gtttttgaaa gtgattcatc agaaaatcac tataatggca aatactatca
     2101 tcaacaccat caagtacatg agtttaaatc aattctctta acttcagatc aagacataaa
     2161 aaaagatttt ttactccaat ggattgactg gctgatttta accaaccaag ggagcctgta
     2221 tcgttttaaa ggattgattg ctttacaaga gcaggatttt atagttgcca tgcaagaggt
     2281 aaacgaacag gttaattttc aatacaccac tcgacaaaaa gaccaaacca ctattattct
     2341 aatcggaaaa atgttggatg aagaaaggat caaacagagt tttgataaat tactggcgga
     2401 aagcaaataa tgataagtag tgattggggg taatttctaa tggagaaaac ggatatatct
     2461 agtgcatatc gacgacttaa aagcccgaat attaaaacta gaaaaagggc attaaaaatt
     2521 atcaaagaac ataaacgaaa taaaatgaag aaattagcat aattaaggaa ggaaaaagaa
     2581 tgaagaaaat cagtgtagga cttattggga tagcagctct aggattattg ggagcttgct
     2641 ccagcactaa tgatgcaaaa gtatcgaatg ataaagatgg aaaattagaa attgtaacaa
     2701 cattctatcc aatgtatgat ttcaccaaaa acatcgtcgg cgatgaagcg aacgtggatt
     2761 taatggttcc agcgggatca gaacctcatg actatgagcc ctcagctaag gatatggcaa
     2821 aagctcatga tgctgatgtt tttgtatatc ataacgaaaa catggaatca tgggtaccaa
     2881 aagctaaaga aagttggaag aaagctgggc ctaatgtagt agaaggaaca aaagacatga
     2941 ttttattgcc aggtagtgaa gaagaagatc atgatcacgg agaagaagat caccatcatg
     3001 aactagatcc tcatacgtgg gtttcaccca aaatggcaat taaggaagtt tcaaatatca
     3061 aggatcagtt agttaaattg tatccgaaaa aagcaaaagt atttgagaca aatgctgaaa
     3121 agtatttgac taaactgaaa cggttggatg ctgactatac cactagtctt aaagaagcga
     3181 aacaaaaaag ttttgtgacg caacacgcag cctttggata tttagctttg gattatggat
     3241 tgaaacaagt tcctattgct ggactaagtc ctgaagaaga gccatcttct ggtcgattag
     3301 ctgaattaaa agagtatgtt aaaaaaaata agatcaatta tatttacttt gaaaagaatg
     3361 ctaatgataa aattgcaaaa actttggcca acgaagctgg gataaaattg gaagtactga
     3421 atcctcttga aagtcttaca aaagaacaaa tggataatgg tgaagattat gtttcagtaa
     3481 tggaagacaa tctaaaagcg ttagaaaaaa caactatggt tgctggtgaa gaggtagttc
     3541 cagaaaaaga agcgaaggat gaaaagacag ttgctaatgg gtactttaaa gatgccgatg
     3601 tgaaggatcg cgaattgagt gattatacag gcgaatggca atctgtttat ccgttgttaa
     3661 aagatggaat tttggatgaa gttttcgatt acaaagcaaa aatcaataaa gacatgacag
     3721 ctgccgagta caaagattat tatactactg gctataagac agatattgat actatcaaca
     3781 ttaaagacaa caccattgat tttgtagtca acggtgaaca tcatcaatac acatacaaat
     3841 atgttggata caaaattttg aattatgaaa aaggaaaccg tggtgttcgt tttaattttg
     3901 aaactgatga tgcaggtgct ggtcgtttta aatatattca atttagtgat catggaattg
     3961 caccaagtaa ggcagagcat ttccatattt tctttggtgg agaaagtcaa gaaaaactct
     4021 ataatgaatt gcataattgg cctactttct atccagcttc attgtctaaa catgaaattg
     4081 ctcaagaaat gatggctcat taaaattcaa tttttggagg aaatacatgg taagaaaagt
     4141 attattgtct atggattcca agaatattga tgcttatcgg cgttatcgat ttgcaaagca
     4201 aatgagatta aatgggagta cattagctga tattgctgca actttacatg ttagtattga
     4261 cacagttcat agagtattaa aacgggatcc tgattgttgt tttatcaagg tcaatactgg
     4321 aagaagtaga cgtttagcaa aacggcataa tagcttggcc aattaattga taataccaga
     4381 gtagatcatt tttcccttat ttggatttga tctgcttttt attatgtctt gaattgtcaa
     4441 atcaagcgca acactttatc aaagaagact tcataaggtg ttctccaatc taaacacttt
     4501 ctaggtctgt tattcagttt ttcaatcatc tgatttatcg ttgagttatc taccgtagct
     4561 atctctttcg attttggcaa gtattctcga atgagtctat tcgtatttta atttgttcat
     4621 tgttgccaag ggcatgagga tctggaaaat aaaagggcat attattcaat gcttctgtaa
     4681 ctgcgaaatg attggagaat tcttttcctc tatctggagt gatcgttttg accgcctcat
     4741 ttggaatact gcttaaaagt tcaatcattt tctcgctcac ataagcggct ttctttctgg
     4801 ggaccttctt tcctgctaaa aggaagcatg attttctgtc tgtcaaagtc actaaacaag
     4861 taccaccagt tttgccagct acaatatcgg ctttccaatg accaaaatca cttctatcat
     4921 ctgcggattt tggacggcca tggatcgaat gagtaaggca aatctttcca cgagactcga
     4981 catgattttt tgtatgtctt gtttttcctc gatggtgcaa tgaacggatc aggcctttat
     5041 tgccgtgtga gaggggttct gtatcgaaaa gatcattgca aattgcacgt tagatcgtga
     5101 taaaactaat tgaaaagtcc atatgttcat gcttcaaacg attcgagatt tcctctggcg
     5161 accactgaaa atcgacaaat aaatgacgaa caattgacca aactcacgga tcatttagaa
     5221 tgtgataacg tccgcatttt tttcttcgat aagcataatt tttttccgct ttggaaggtg
     5281 aatagttttt ttcttgtttc ttctcagttc atgtaaaata gtaaacgggc tatgctgaat
     5341 gtcgttgcca attatactaa tagaaaatcc ttcttcgtga agcagaaaga ttctttctcg
     5401 ttcgagtata gtaagatgac gatactggct cataattatt ttccttctag gagtgttctc
     5461 gacaaactta tactagttgg atatcgatta tgactcattt ttatgtgttg cacttaaagt
     5521 gtaaattcaa ggtatagaaa gaggttccct atgaaaaaaa tacctattac aatcttaact
     5581 ggctatcttg gttcaggaaa aacgatactt ctaaatcata ttctgtacgg tgatcacgga
     5641 aaggaaattg ctgtcattgt aaatgacata ggggaagtaa atgttgatgg ggtattagtc
     5701 gacaagaaag gttttaaaag aactggagaa aaacttgttt cgatgccgaa tggatgcatt
     5761 tgttgtacat tacgcgaaga tttgattgtt gaactagatc aattagtgca attttctgac
     5821 ttgaatcaaa ttgttattga agcttcagga atcagcaaac caattccaat tattcaagca
     5881 attatattag ctaaaaaccc tttagacatt gatttgaccc aacgattaga gattggtgcc
     5941 attattatag tagtggatgt aaaacgtttt tgggataatt tcgaaacggg tgaaaagatt
     6001 caggaaagaa aagctgaagg taatgtagtt gaagaagaac aggatattcg tggcttgtta
     6061 attgatcaat tcgagtgttg taacatatta cttttaaata atagtgactt agtgtcaacc
     6121 aagacgattt acaaaattaa cggtctaatt gctaagttac agtccaatgc caaaataatt
     6181 aggacaatta aaggtgaagt agacttatcg aaaattctaa atacaaatct ttttaatata
     6241 gatgagactt cctattcagt gggctggttc aaagagttag agcttggcta tcaaaatcat
     6301 attcctgaaa cagaagaata tggtatctca agttttatct atcgaaatcg gttactcctt
     6361 gacgcacaaa aatttgccca tcttacggat aaaaattttc ctggcaatat tacaagagca
     6421 aaagggtttc tttggtttgc ccaacatagt gatttttcga cgatctttga aacatcgggt
     6481 agctataaaa gtattgaatc attaaattat tgggtagcta gtttacctaa aattgagtgt
     6541 caaatgatta tacaaaaaaa tcccgaattg aaggaagcct ggcatccgtt gtttggtgaa
     6601 cgcattaata aactagtttt tattggtatg aagatggatc aacaatgtat taaggaacaa
     6661 cttgagggtt gtttaactga ttctcttaat gcagataatt tccgaaaaat ttcaccattt
     6721 ccatggaaaa agtctaatat gtttagccag atatcgttaa tgaaattttt aagaaaagct
     6781 ggtgtcaaga taggcgatgt cataattatt ggcattttga ttattacctc ttttattccc
     6841 ttatttgtat tctctataga aaaggagaat gaaacaactg aagatgctat caaatatgcc
     6901 atcgttcgaa ttgatggcaa ggaaatggat cgcttcgatt tggataagat caatcataaa
     6961 ctcgtgaatt atcatcctgc gaagggacaa tataatattg ttgagattaa agatggaaga
     7021 attcgagtta aagaagataa tagtcctgat caaatcgcgg ttagaactgg atggatctca
     7081 aaacccggtc aaacgagtat ttgtttaccc cataagttag ttattagtat tgagaaaaaa
     7141 gaaagcacag attactacat ttactaatct ggaaatagat gaggaggttt ttaataagaa
     7201 aaagatatta ttattaataa tagcaggggc tttattaagt gtgttaagcg cttgtggaca
     7261 agaaaaagaa gcggtgacaa caggcacaag tatctttaac gggacctgca atggtttagt
     7321 taacagtcgg ttagattact tattttggct cttcgtcaat gagtgttggt gaagaaattc
     7381 aatagaataa tcgagatact cacacggcta gacagctatc ttgtctagcc gtgttgttgt
     7441 tctggcttta gtcagttggg tagtgttctt agttttcttg aaatattttc ctcgatataa
     7501 aaagattcgt tctcggtagt tttgaaagtt tctaaagcca taggcaattc gttttagtac
     7561 tttgatatgg ttgttcaaac attctatagg gccgttggaa tagggaactc gcaacgctcg
     7621 tttgatttct gttcgatatt tcttgaaggt cgtgaacact ggttgatagt gctctggcaa
     7681 ctccttgtag tcttctttta ggagcgcgac gaattcagga acatcttgtc ttctgatagc
     7741 ggaaagaaaa acctggtaga cttcgtagcc tcgctttaag gagtcatcat aatataatag
     7801 ccgatcgact atctccgctt ctgtcagatg atctcgaaat gacggacgcc agtagcgatg
     7861 ctgaccattc aattcccacg cattcttttg aagtagtctc cagtatttct ttaagtgctt
     7921 cgaccgacga tccccttttt taaaggtctt cattacttga attcggtggt tggtaaaggc
     7981 ccgaccgatg tgttggctaa tatgaaagcg atcggcgacg attcgggcat tcggaaaaca
     8041 ctctttcacc aatgtgcgat agggtgtata gaaatctgaa accactagac gtacttgttc
     8101 acggactttt ctagggtagc gcaagaaata gtcgcgtaaa ggattgagtt gccgattttc
     8161 cacaatatca atcagttgat gggtcttgcc gtccatcatg acaaagctca tggctcccga
     8221 gacgtttttc accgatttga attcatcgaa acacaccact tctggtaacg gctggttcaa
     8281 agaaacggtt ttaggccgaa ggctgcgaag tattcgaatg acagtggtcg gagaaatgtg
     8341 cttcatacgt gcaatcaaag acatagaaat aggctcactt aatagctcaa gaatggcttg
     8401 tttgactctg cgggcaatgg agcagcgacg atcgctaact gaatcttctg ctaaaaaagt
     8461 ctttccacag tgtttacatt tgaagcgctg tttgttaatc cgcacaatgg tcttgtagtt
     8521 actgacatca ttcaataaga tggatacttt tttccagccc catttaatga tcgtttgctt
     8581 attttgatag tggcaatgga agcattcctc tggttgatag gtcaatgtgc catcaaaaac
     8641 aaaacatgtt tcgtcattaa tgcgctcttt gcgaaaggca tcttcatgaa aggtaagatt
     8701 taggtctaga atatctagta actctttggt aaactctgaa taggacatct tgaaacactc
     8761 ctttgaatgg ttttcgtcga ctttattcta aaggatttca aggtgtctgt ttgcgtttat
     8821 tagaaaaaag aaaaagtatt ggcaacagaa ggcgtcacca acactagata ttatagatcc
     8881 agaaaagcag attgtttatg ccaaaattaa gacattgcaa aaagattatt ttctttaaaa
     8941 tcgacaagat gaccagaaag caagagttcg ttgagctctt gctttctttt agttttagaa
     9001 aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa gttttgcaga ttggtcgtag
     9061 ataatcggtt ttttcaataa agaaggggat gtacgaatct caatatatag ttcactcaaa
     9121 ctgagttcat ctattctgtg aacaagattt ttataactaa tagaatgagt agagagcatt
     9181 tcggtcacgc cgttgtttgt caattttagt aagtggataa attcattttt ggttagtggt
     9241 tgacgaatta agttctgttc gataaacggt atatcatgga cgataagcca acgttttgcg
     9301 cgtcttgaag acgagcagcc agaacctgta tacaatttaa tcatcataca ttcctccaat
     9361 agctaagtat ttatgaaata ataacatgaa gaaatagtcg aaaagtgcca tttagaggtg
     9421 taaaataaga aaatgttgtc acgtataatg gaactagcta gataagaaat actattaaat
     9481 caagaaaaaa gaaagaaggc acaagtatgt caaacacagc cgacgcaata gtagtgatcg
     9541 atttacaaaa aggggtatgt tttgattctt cgccgataca cgaattagaa acagtgacag
     9601 cattagtgaa taaaagaatc aaaacatacg aaacgattgg gaaaccagtt attttcgttc
     9661 aacacgaaga tgctcaatta gtgccacaaa caaaaccatg ggagctattg tcggaattat
     9721 ctattcctga gcacagttat tttgtgagaa aaacccatgc gaattcattt tataagacta
     9781 atttgagtgt tttattaact gaattggact gtcattcgat cgaattatgt ggggcacaag
     9841 cagagtattg tatagatacg actgtgaagt ttgctcatgg attaggatac cagcttatga
     9901 tgtgtagagg agcaacgaca acttatgata atccttttat gaaagcaaag gaaacaatcg
     9961 ctttttacga gaaaatttgg gataaacgat ttctgacatt tttggagcaa taagttgttt
    10021 cttcgtttaa aaaaagcaga atggatactg gtttatccat tctgctttat tacgtaaaat
    10081 gagaaaataa tcctgttcta tttttatggg ataaaccatt tattttttgt ataaaaaatg
    10141 tttgcgttta catataattc tcctagaaac ataaaaagga gtgaatagct tgagaacaaa
    10201 acaagtatta aagggagcgt tggtcatctt atttgctgta agtacaagcg tcccgcaatg
    10261 gacagtatat ggagagagca tttcttccga atcggaatct cgggaggtag aaaccacaga
    10321 aactactgat gctggaaatg aaacgaaatc gagtacttca caaacagaaa caaatactga
    10381 aacaacggtt gagacatctg ttactagcac agatcaagaa acgaccgcta ccacagataa
    10441 ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac gacgaatcct cttctgctaa
    10501 agtaagtgac aaagagaatg ggacagaaaa tgatgatatc ggtaaaggtg aaaatttcaa
    10561 tggtaaagaa tatttgaatt acttcggtaa acagatgaca gaaatactta atggaccaac
    10621 agaagacatc aagtcaaaat taatagagtt attagtcact gagcttgctg ttaatgatta
    10681 tggtgtagat aaagagatta tcaaacatct gattgctact tatctattgt acgatatcgc
    10741 ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt acagatgatg ggacaacgcc
    10801 tacagaaaac acaggcttct atccaaatac aaaacaactt catcaaactg tactaaacaa
    10861 acaagatggc aaaatgatgg atgtatatgc atattatgtc gatcaaggtt cagataaaac
    10921 agtcatgatc catggcggat ttcgagggaa ttggaataac gggatcgtta cagaagaata
    10981 caatgatttt tataaggccg ggtataattt attatttgtt gattccagag cgactggtaa
    11041 tagcggaggc gattatgtga cctatggaca atatgagtca gatgacgtcc tttactggat
    11101 aaatcaagaa gttcgagagc ggccatctca aaagatttta ctttatggtg gttcgatggg
    11161 agcagctacg atgatgtctg tattagcgaa agatattcct gtcaatgtca aaggaattat
    11221 tgaaaattgc ggatttgcat caattgatga acaactacgt tttacgtaca gccagacagt
    11281 agcccccgca ctaccggatg ccataaaaaa tcagttagac atcattgggg atcaagagca
    11341 tgaagattta tttatgggcc ttttgaaaca atactatttt gatcaggaga tgcatttgga
    11401 tacaaaagct gctctaccta cgattggaat gtcagattca ttgcctaaat tgattatcca
    11461 tggaacagca gatgatgtag tacctgtgtc taacgcgcaa aaattatatg agttatcagg
    11521 agggtataaa gatcttttat tagtagaagg agcagggcat ggaaaggcac aggaagttga
    11581 ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa gtagtgttcc atgatcagat
    11641 aaacgtaaag tatgtggatg aaaacaataa aagtctactc aaagaccagg acgaaatcca
    11701 gctttacgga gcttatggag aaaattatat gacagagcaa aagacattcg aaggatacga
    11761 attagcaaat gttgaaggac cgactgaagg gatttttaat gagacgatac caactattat
    11821 tttctattac aaaaaaatcc cagtagctcc accaaaaaag caagatccga cagagaatgc
    11881 agacaataag ggaaagaatg aacctcgaga ggatcaatca agtgatgata cgcaaccgat
    11941 tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa aaaagaatgt tgccgaaaac
    12001 tggagaaaag aaacattcgc ttctcattta ttcaggtgga tttttatctg ttagcacgat
    12061 tatcacatgg atttggcgaa gaaagagagg gtaaatgaat gaaatggttc aagcgaatag
    12121 tgattgcagt accagttatc atcgtagtta ttttagtcac tgtatttttg attaatcaaa
    12181 tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt tgacacaagt gaaaaagaac
    12241 aagtatatgc ccgaccagat gatttccagc aaaaaatcga acaaatcgat ataaaaaaag
    12301 aaatatccta tccttcaaag tacaaaaata ataatatgga tatctacaca ccgaaagtgg
    12361 aaaagaaaaa attaccgatt cttttctgga tgcatggagg cgcatatgta ggaggggata
    12421 aaaatgattg tcgtgattat ttggaatatc tatgttcaga cacgcagcaa atcatcgtaa
    12481 atatagatta tgaacgatca ccggaagcaa aacatcctac accagttatc caattgaacg
    12541 aagccattca agcagctaaa aaagagataa aagatcaagc agattggtcg aatatttcga
    12601 ttggtggaaa ttcggcaggt gcacaaatta gtggagaata tttgttagca cttcaaaatg
    12661 aagaaattaa aaaagctgat catcttgatc cgactttgga gaacaagcag attacgaagt
    12721 ttatttcttt gtctggtctg ttggatcctt ctgcacttac tcaagtaagt gataaaatct
    12781 ctagctttct ttatgaaaaa tgtggatggg cttactttga taataaggac tttgaacaat
    12841 cagagaaggt caaaagttta gccttggtca gacatgcgga tcgttggacg caaaacacat
    12901 tcttaacgga tggaaataca aatacgttta cgaaacaaat ggaagaagta acttctgtat
    12961 ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc ggagaaaaat gctgttttga
    13021 accatgaata tcagtttgat ttcagcaata aacaagcgca agaaacatat caaaagttag
    13081 taatgttttt caaggagtaa agtcttatga tgcaaaagag gttgtaatct gtcacttagc
    13141 tccaagtcac aaggagcaat ttcaataaac aagcttttca tggtttatga atattgtaac
    13201 ttgtgactga ggagttagag atggcaaggg gcacctttta ttcgaaattt gggaatatca
    13261 cacgattatt gtcacgatct cttttacgta aattttataa gtcagatttg ttgttttgat
    13321 cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa gaagggacaa aaagccgagt
    13381 ggtaagcaaa agaatgctgg ttccactaat attccgttgt tattgatata aggctgggaa
    13441 caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa aaagcaaaga aatatggata
    13501 gctttcataa gcagcacctc cttttttata attgtatgat aaatagatta agaatctata
    13561 aatgtgacaa gtttgttagg atgtgaataa aatgatcatc tagcgaactt gaaacaaaga
    13621 tggctaatga taagcaatta atgattcagc aatcagatag gtacaaagtt taccatcata
    13681 cgtctagacc aataatagaa aat
//
LOCUS       DAPCMT010000073        13701 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPCMT010000073 DAPCMT010000000
VERSION     DAPCMT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.073"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..902)
                     /locus_tag="RD743_001749"
     CDS             complement(<1..902)
                     /locus_tag="RD743_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDU0563559.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="RD743_001750"
     CDS             1185..2153
                     /locus_tag="RD743_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0563560.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="RD743_001751"
     CDS             2266..2544
                     /locus_tag="RD743_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDU0563561.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="RD743_001752"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="RD743_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDU0563562.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="RD743_001753"
     CDS             3001..3756
                     /locus_tag="RD743_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDU0563563.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="RD743_001754"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="RD743_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDU0563564.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="RD743_001755"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="RD743_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDU0563565.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="RD743_001756"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="RD743_001756"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDU0563566.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="RD743_001757"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="RD743_001757"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDU0563567.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="RD743_001758"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="RD743_001758"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDU0563568.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="RD743_001759"
     CDS             8493..9035
                     /locus_tag="RD743_001759"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDU0563569.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="RD743_001760"
     CDS             9058..10614
                     /locus_tag="RD743_001760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDU0563570.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="RD743_001761"
     CDS             10628..11530
                     /locus_tag="RD743_001761"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDU0563571.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="RD743_001762"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="RD743_001762"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDU0563572.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="RD743_001763"
     CDS             12973..13395
                     /locus_tag="RD743_001763"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDU0563573.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2451 c         2988 g         3732 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag a
//
LOCUS       DAPCMT010000074        13429 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPCMT010000074 DAPCMT010000000
VERSION     DAPCMT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.074"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..914)
                     /locus_tag="RD743_001764"
     CDS             complement(<1..914)
                     /locus_tag="RD743_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDU0563574.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="RD743_001765"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="RD743_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDU0563575.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="RD743_001766"
     CDS             2006..2176
                     /locus_tag="RD743_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563576.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="RD743_001767"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="RD743_001767"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDU0563577.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="RD743_001768"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="RD743_001768"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDU0563578.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="RD743_001769"
     CDS             4440..5321
                     /locus_tag="RD743_001769"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDU0563579.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="RD743_001770"
     CDS             5318..6034
                     /locus_tag="RD743_001770"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDU0563580.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="RD743_001771"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="RD743_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDU0563581.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="RD743_001772"
     CDS             6751..7752
                     /locus_tag="RD743_001772"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDU0563582.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="RD743_001773"
     CDS             7749..9110
                     /locus_tag="RD743_001773"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDU0563583.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="RD743_001774"
     CDS             9070..10221
                     /locus_tag="RD743_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDU0563584.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="RD743_001775"
     CDS             10196..11365
                     /locus_tag="RD743_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDU0563585.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="RD743_001776"
     CDS             11540..12919
                     /locus_tag="RD743_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDU0563586.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="RD743_001777"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="RD743_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAPCMT010000075        13398 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPCMT010000075 DAPCMT010000000
VERSION     DAPCMT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.075"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(14..706)
                     /locus_tag="RD743_001778"
     CDS             complement(14..706)
                     /locus_tag="RD743_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDU0563587.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="RD743_001779"
     CDS             complement(1143..1712)
                     /locus_tag="RD743_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDU0563588.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="RD743_001780"
     CDS             complement(1786..2109)
                     /locus_tag="RD743_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563589.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="RD743_001781"
     CDS             complement(2263..3075)
                     /locus_tag="RD743_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563590.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="RD743_001782"
     CDS             3429..4277
                     /locus_tag="RD743_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDU0563591.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="RD743_001783"
     CDS             4422..5363
                     /locus_tag="RD743_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDU0563592.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="RD743_001784"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="RD743_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="RD743_001785"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="RD743_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDU0563593.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="RD743_001786"
     CDS             complement(9827..12013)
                     /locus_tag="RD743_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDU0563594.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="RD743_001787"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="RD743_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDU0563595.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="RD743_001788"
     CDS             complement(12235..12675)
                     /locus_tag="RD743_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDU0563596.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="RD743_001789"
     CDS             complement(12749..13288)
                     /locus_tag="RD743_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDU0563597.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DAPCMT010000076        13369 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPCMT010000076 DAPCMT010000000
VERSION     DAPCMT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.076"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            167..961
                     /locus_tag="RD743_001790"
     CDS             167..961
                     /locus_tag="RD743_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0563598.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            951..2993
                     /locus_tag="RD743_001791"
     CDS             951..2993
                     /locus_tag="RD743_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0563599.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            3274..3423
                     /locus_tag="RD743_001792"
     CDS             3274..3423
                     /locus_tag="RD743_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDU0563600.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            3434..4948
                     /gene="dltA"
                     /locus_tag="RD743_001793"
     CDS             3434..4948
                     /gene="dltA"
                     /locus_tag="RD743_001793"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDU0563601.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            4945..6150
                     /gene="dltB"
                     /locus_tag="RD743_001794"
     CDS             4945..6150
                     /gene="dltB"
                     /locus_tag="RD743_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDU0563602.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            6195..6428
                     /gene="dltC"
                     /locus_tag="RD743_001795"
     CDS             6195..6428
                     /gene="dltC"
                     /locus_tag="RD743_001795"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDU0563603.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            6431..7699
                     /gene="dltD"
                     /locus_tag="RD743_001796"
     CDS             6431..7699
                     /gene="dltD"
                     /locus_tag="RD743_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDU0563604.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            7840..8922
                     /locus_tag="RD743_001797"
     CDS             7840..8922
                     /locus_tag="RD743_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDU0563605.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            8922..9725
                     /locus_tag="RD743_001798"
     CDS             8922..9725
                     /locus_tag="RD743_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDU0563606.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            9795..10535
                     /locus_tag="RD743_001799"
     CDS             9795..10535
                     /locus_tag="RD743_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDU0563607.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            10546..11550
                     /locus_tag="RD743_001800"
     CDS             10546..11550
                     /locus_tag="RD743_001800"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDU0563608.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(11595..13292)
                     /locus_tag="RD743_001801"
     CDS             complement(11595..13292)
                     /locus_tag="RD743_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563609.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         4300 a         2190 c         2702 g         4177 t
ORIGIN      
        1 ttattgtgaa aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt
       61 tgctgaataa aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca
      121 taagagcaaa ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag
      181 aggaggagaa gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag
      241 caaactttct cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt
      301 tgttgggatt atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac
      361 gatcaatttg cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa
      421 aaacagtgag ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa
      481 tttgatcgat accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat
      541 gactaaagag gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga
      601 actgctcaaa cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc
      661 tagagcactt gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga
      721 ctcgaagtcg gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc
      781 aacgatactg ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt
      841 tattaaagat ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt
      901 tgagaaagta atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata
      961 aactagccgt taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt
     1021 tgactttatc cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag
     1081 tcagaagagc aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca
     1141 ttactgttgt tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc
     1201 aaaaggaaat aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat
     1261 atgtgttaga aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta
     1321 ttattttttc caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta
     1381 gtccattttt tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc
     1441 ttttagcagt atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt
     1501 ttggagaaaa agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac
     1561 tcgcagtcat cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg
     1621 aaatcacttc atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt
     1681 ttttaatttt tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt
     1741 tatttttgat catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa
     1801 tgggtaacac gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca
     1861 ttctcggaat ttctttaatg acaattggcg gcacggctag tatctctact attttatcct
     1921 accaggagaa agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa
     1981 tcaagccgat tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata
     2041 aagtcattgg gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc
     2101 tgatcacgga aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt
     2161 ctttgaaatc acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata
     2221 tttcattata tggatctagt attggactgc cagataatca agaattgaag attcaacaaa
     2281 tgcttccaag tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag
     2341 atgataccgt ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat
     2401 caggtggaaa taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc
     2461 tcaatgctgt ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca
     2521 caaagaaact tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt
     2581 tgaatttctc ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata
     2641 tttttgtcac actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag
     2701 tccggcaatt agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg
     2761 ggattccaca aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc
     2821 cacccatgat attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata
     2881 taaaaactgc cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc
     2941 ttttgcttta tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg
     3001 aataagacaa gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt
     3061 tgtgacacga tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat
     3121 tataaagaaa aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg
     3181 ttagtaaaaa aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac
     3241 actttgtgtg aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa
     3301 ttgaattatt ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc
     3361 ctgatttatc tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt
     3421 taggaggaag agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa
     3481 tgaacctgat cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca
     3541 agcatcagac gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt
     3601 cgtttttggc aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg
     3661 ccatgcctat ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg
     3721 ggtggctaaa ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt
     3781 tcctgtcata acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc
     3841 cagaaatagc gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg
     3901 agaaccaaaa ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat
     3961 ggaagacttt gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga
     4021 tttatcggta ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa
     4081 taaagcagtt gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa
     4141 tgtgtgggtt tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg
     4201 agaaaatgtg ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac
     4261 tgcagaaagt ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac
     4321 agaagcaact gtagctattt caagtattca gattgaccag catgtactag atagttatga
     4381 ccgactaccg attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa
     4441 acctgtttca aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg
     4501 ttacctgaac aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag
     4561 ttaccataca ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg
     4621 tatggatcag caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta
     4681 tctgctccaa gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa
     4741 agtacagcag ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca
     4801 gttgactaaa tctatcaaag aacaattact agaagttgta atggattata tgattcctca
     4861 gaagttccat tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa
     4921 attaacagct gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg
     4981 ttttattttt ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg
     5041 gaaaaaagat taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt
     5101 ggaggtaatt attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata
     5161 cttgtatggg gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc
     5221 gccgttattt tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat
     5281 ggaaaagcat cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa
     5341 atgatcatgg aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag
     5401 tttctgctgt tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa
     5461 aaagatcagc tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc
     5521 caaaatattt ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta
     5581 ctgcttcctg tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta
     5641 ggttatatgt acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt
     5701 gcagtcggta caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt
     5761 ttatcatgga atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc
     5821 cgtgattatg tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc
     5881 aagatcgtaa tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat
     5941 ggattgacgt ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca
     6001 gatgcatgga tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg
     6061 catggcttag ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca
     6121 ggatttttag ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa
     6181 aggagaaaag gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag
     6241 atgaagtaag aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag
     6301 catctgttca attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg
     6361 aattcgagcg agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga
     6421 agggttgagt atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg
     6481 cttactgttt gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat
     6541 tagacgtgcc tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta
     6601 tgcattatct gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag
     6661 tccatttcat ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct
     6721 gggagcaccc gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc
     6781 tatgaaaaac aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt
     6841 aaaaactgat tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc
     6901 aatgaagaaa gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa
     6961 agtaaaagag aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc
     7021 tttacctgaa tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga
     7081 agtattcagc aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg
     7141 attaccaaaa caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg
     7201 agaaacgacg aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc
     7261 ccacgagcca gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt
     7321 ttctgatttt cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat
     7381 catcccaccg gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca
     7441 aggatttgcc aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga
     7501 ttttgtcaat caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa
     7561 aggctggttg gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc
     7621 tagtaagtat catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga
     7681 taaactacaa ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt
     7741 gaatattctg aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg
     7801 gtataatcac aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag
     7861 ctacttattg atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa
     7921 gtattcagag aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt
     7981 gtgattgcca aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac
     8041 gaagttggtt ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt
     8101 ggaggttggt gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg
     8161 aaaatcgtcc acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga
     8221 aataaacctt atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa
     8281 gccaaagagt ggggcatacg tcctggagat atggtcactc catatattga atacaaacga
     8341 atgaatggca gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt
     8401 tctctgcgag tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga
     8461 agcgatgtgc aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat
     8521 cccgatattg cttttgcttt agatactggt acagcaggag acactccagg aatgacacca
     8581 aaagaagcag attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg
     8641 atcccgcata aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca
     8701 ttccaatata cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga
     8761 gatggtgttc cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca
     8821 gtgattcatg aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg
     8881 cttgatagtg aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta
     8941 tttctgaata tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt
     9001 ggcttgtttt cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca
     9061 gtccttttcc ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac
     9121 ttttattcgc attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca
     9181 gaccaaaaag taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg
     9241 gcatcaattt gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca
     9301 tgtttttaat cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac
     9361 ctacgggtgt catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga
     9421 tgatttttgt ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat
     9481 ggacaaagtt cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt
     9541 tattgaaaga tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt
     9601 tgctaggagc aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt
     9661 cagttgcaga attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta
     9721 cttagcatgt aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg
     9781 agggggaatg tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa
     9841 atcaagggac aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag
     9901 gtagtgctac gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga
     9961 tccttagcca ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc
    10021 aacttcatcc taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt
    10081 ccattcactt tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag
    10141 ctgaagaatt aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag
    10201 tatgttacgc catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg
    10261 attcaggtta tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag
    10321 atacctatct ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg
    10381 gagaacttgc gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg
    10441 gtgatttaga acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag
    10501 aacttgctgc agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca
    10561 aatgaaaacc acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg
    10621 cctttagatg aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga
    10681 aaccaaaata cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt
    10741 gtccgtcgct taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt
    10801 tttatcatgc ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc
    10861 atccaagcac ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta
    10921 gtcattgatg gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt
    10981 gctcatggaa caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta
    11041 aaaaaagaca aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc
    11101 aaaccatttc ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc
    11161 ttgttgaaaa ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat
    11221 gaagattgga aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat
    11281 tacgggaagt ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt
    11341 gaagtccatt tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc
    11401 ttcggactag gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa
    11461 gcatctctat caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa
    11521 ccagaagaat tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg
    11581 gattccgcta ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga
    11641 tcaatgaaag tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc
    11701 atcatcatta agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat
    11761 aggtttgtat cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg
    11821 tgtatcgaca aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag
    11881 gaatgctcta aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct
    11941 tcgggaagat ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa
    12001 cgcgctaaga taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt
    12061 tttgcttctt ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt
    12121 aaatcttgca gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg
    12181 atatgctgtc gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa
    12241 tctacttgtc tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata
    12301 ataacgatta gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata
    12361 cctaatgccg tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt
    12421 gtacaagcta cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg
    12481 actaaagtgc ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca
    12541 ggagtaaaag ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa
    12601 attccgtacc attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct
    12661 gacgttaaga taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata
    12721 ccacttaaaa tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag
    12781 agaataagtg agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag
    12841 gtccctatag cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa
    12901 taaataaaac taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca
    12961 tagttctgat aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt
    13021 aaatctatca cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg
    13081 tataaaatct tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa
    13141 tttgcttcgt ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc
    13201 acggtaggaa tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta
    13261 tagaaaaccc acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata
    13321 aaataaaaga aataagcaat tcctgttaaa aataacaatg ataatataa
//
LOCUS       DAPCMT010000077        13351 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPCMT010000077 DAPCMT010000000
VERSION     DAPCMT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.078"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            2..169
                     /locus_tag="RD743_001802"
     CDS             2..169
                     /locus_tag="RD743_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDU0563610.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(231..2042)
                     /locus_tag="RD743_001803"
     CDS             complement(231..2042)
                     /locus_tag="RD743_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDU0563611.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2039..2404)
                     /locus_tag="RD743_001804"
     CDS             complement(2039..2404)
                     /locus_tag="RD743_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDU0563612.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2567..2962)
                     /locus_tag="RD743_001805"
     CDS             complement(2567..2962)
                     /locus_tag="RD743_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDU0563613.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2980..3942)
                     /locus_tag="RD743_001806"
     CDS             complement(2980..3942)
                     /locus_tag="RD743_001806"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDU0563614.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3942..4154)
                     /locus_tag="RD743_001807"
     CDS             complement(3942..4154)
                     /locus_tag="RD743_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDU0563615.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4175..4873)
                     /locus_tag="RD743_001808"
     CDS             complement(4175..4873)
                     /locus_tag="RD743_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDU0563616.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4893..5435)
                     /locus_tag="RD743_001809"
     CDS             complement(4893..5435)
                     /locus_tag="RD743_001809"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDU0563617.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5567..6571)
                     /locus_tag="RD743_001810"
     CDS             complement(5567..6571)
                     /locus_tag="RD743_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDU0563618.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6568..7557)
                     /locus_tag="RD743_001811"
     CDS             complement(6568..7557)
                     /locus_tag="RD743_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDU0563619.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7554..8360)
                     /locus_tag="RD743_001812"
     CDS             complement(7554..8360)
                     /locus_tag="RD743_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0563620.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8526..9482
                     /locus_tag="RD743_001813"
     CDS             8526..9482
                     /locus_tag="RD743_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0563621.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9559..10077)
                     /locus_tag="RD743_001814"
     CDS             complement(9559..10077)
                     /locus_tag="RD743_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDU0563622.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10165..10314)
                     /gene="rpmG"
                     /locus_tag="RD743_001815"
     CDS             complement(10165..10314)
                     /gene="rpmG"
                     /locus_tag="RD743_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDU0563623.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10542..10988
                     /locus_tag="RD743_001816"
     CDS             10542..10988
                     /locus_tag="RD743_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDU0563624.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11183..13078)
                     /gene="asnB"
                     /locus_tag="RD743_001817"
     CDS             complement(11183..13078)
                     /gene="asnB"
                     /locus_tag="RD743_001817"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDU0563625.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4158 a         2831 c         2277 g         4085 t
ORIGIN      
        1 aatgaaaaaa tttgtatctg gaatattagt aggaagtctg gcaacagccg ctgccgttgc
       61 tggtttggtt gcttctgtga aaaaaacagt gatcgatcca attgacgaaa aagaagcaat
      121 gatcgaagaa aaccgtaaaa aagcaatgcg taaacgcgtt tctcgttaat aattatttaa
      181 aaatacgcat gaagactttc taaaaaacag ataagtcatc atgcgtattt ttatttgtca
      241 gtcaatataa gttgtgctat tttatctgcc tgtctcagat tccatggatc tgtataaggt
      301 ctagctactg ctgcagttag ccaatccaca ccttcaacag gtattccata gcaataaaag
      361 tcagactgga tttcccctga agcatcaatc acgcgattcg tacctcgctc aacatctata
      421 gcttctgaat gataacttcc tgtattatct gaaaatgaaa aagggcgaac aagtttgttt
      481 tccttcaaat tttttatgac aggattactt gaacgagaaa agcttgtttg tggcaatcga
      541 gcttcaatca aatagcgact gctgatttct ttatcttgta gagaaataac atagcggttg
      601 tgcttttgct gaatatcaaa tggcggttcg atcaaatgaa aaatacccgc atctatcagt
      661 gctgctaatt ctttcgtccg aatgattggc ggaccgatcg ttaaaaagct atttagatga
      721 gtaaaccatc cccatagtat ttctttgtat tccttagcag aaaaaacgtg ccattcaata
      781 gcctgccgga ttggatcacg ccagtctttt aatgcatcaa atgttgaagc aaatgctcct
      841 gtgcaattgc ctttttccgc ttcttttatc tgcccttcaa taaatattcg gctagcttct
      901 gaaaagggct cattctgata ggcaagagga gtttctaaat acgaccaatc ccatctataa
      961 gggattagtg ctggatgctt tccgagacac tcctctgaag agagggagag aaaatcatgc
     1021 tcaaaatttt tctttggaat cggcaagtga tattcttcga tgattttctt gtaataaaac
     1081 aactcaacgt cttttttcag atagtcaaaa aatgtttcgc cagtcaaaaa tcccgactga
     1141 tgccattgct ccaggttttc ttttgttagt aaccgtggcc atgccattgc tccgccttgc
     1201 ttttgatttc ttcctcgagg gtaataaggg agcccccttc ttgatccact gtatacaatc
     1261 ggttccttgc ctgatggatg atagaccagt tttccctttt cgttctcgaa gcgccctcct
     1321 cttgatactg taaacaatcc gacataatca aaaaaactta agcctagacc tcttaaaaag
     1381 acattttctt taggcggtat cttcgaaaga tctacatctg caggattaga aggaagctga
     1441 tacagtaaaa catttttctt tgcgtagtct acccacactc tttcttcttc tgtctcatca
     1501 ttttcccagt gaccgcttgc taaaacaagc tgatcgatag aatagctttg ctgatctgtc
     1561 tgtaaaagat aaccatcttt tcttttgctt acttttttta ctaaagaata aacaaactga
     1621 atagaagctg gaaattcttc ttgcaacttt gagaaaaacc agcgttgata gagtccgtat
     1681 aaacatcgag tagattgctg atctttttcc agacgattga tttcttctat aattagttgc
     1741 ttatttttgg ttaccgtctg ttcgatataa tctgtagcag gtcctttact ccattgaaac
     1801 aagttgggtc cctgtgttac caatcctcca ctggacaatg tttcatctgt aaaaagcgtc
     1861 acttgttgag aaacagaatt cattaataac tccggtggct gattcaaccg ccaaactcga
     1921 cctccaggac cattgggatc gaaaagaaaa agttcaagag aatgtctgtc ctgattatta
     1981 tggagaatcc tttctgctac acttaatcct ctaggtccgg caccaatgat ccctattttc
     2041 atttgcatgc ctccatttcg ataaattttt tcaataaaac agcatggttc tgatgttcat
     2101 tttttgcacc ataaacaaaa gtaatgactg gatgttcttt cataagttga ctgagtttaa
     2161 aaatagcttg cttcttttct tcttcgaaaa tcaattcttg ctggtatttc tttgcaaatg
     2221 aagcaaatcg ttctggtaca tggtcaaaag ttttgcgtgc ttcattacta ggtgcaattt
     2281 ctttcaacca cagatctaac tgcgcttttt cttttgaaat tcctcgtggc cagaatctat
     2341 ccacgagtat tctatagcca tcctcttttt cagcatcaag gtaaacacgt tttaatttga
     2401 tcatagttgt ttccttcttt ctaccttttc gcttagctta tatatgtaga taatagcaca
     2461 aaataaaaaa aatgaacggc acaactatcc attgcgccgt tcatgtgatc ttggtcaaaa
     2521 tctaatgatt gattcggttt ttaaagctgt catacaatca tctgttttat acttttttac
     2581 ttttgatttt tccagaccat ccgtcatatc cacccttaag taaataaatc tcttgataac
     2641 ctgctttacg taatttattt gctgtacgga tgctcaaact tttcttttga tcgtaaatat
     2701 agacaggctg gtcttttcga atagatccta atgaatcttt caaaacagtg taagggatgt
     2761 ttcttgcacc taaaatatgc ccagcatcaa acacatcttt ttcgcgaaca tcgattactt
     2821 gtgcttttct cattccttct cgaaattctt cttctgtcaa agtcgttgct gaacgtttgg
     2881 ccattactcg tagatagact tcccaaagga tgattgcaag cacaatcaat agcaaaatga
     2941 tattaattac ccataaaacc atgtaatgcc tccgtttttt taagcaaatt tcaaggctaa
     3001 tgaggcagca ccaatgacac cagcttcatt accaagctct gctaatttga tttttgttga
     3061 ttctcttact tgcgggaaag taaattcttt aaaatatttc tctacacggc tacgtaaaaa
     3121 ttctcctgct gccgatactc ctccacctaa cacgatactt gacggattta atgtattccc
     3181 caagtttccg caagctaaac ctaagtagaa gcatacgcga tcaacaacca tcaatgcaaa
     3241 cggatcgtct tcttgagcca tttcaaagat atctttactt gttacttctt ctccgttgtc
     3301 tagcattgct ttcagttttg aatcccctgc gtattcttct gccaagaaac gacctaggcg
     3361 taccacacct gtagcgctag atacagtttc taaacatccg cgtttaccgc aagtacattc
     3421 gaacccttca ggatccacag tgatatgtcc gatttctcct ccagctccgc tcacgccgtg
     3481 aatcaaattt ccgccagcaa cgatacctcc gccaacacct gttcctagag taacgaatac
     3541 aacgtctgga tcgttttctc cagcaccttt ccatcgttca cctaaagcag caacattcgc
     3601 gtcattatcg atagcgaatt tgattcctgt tcctgcctcg atcagttttt tcacaaattg
     3661 aacttctgtc cagtttaagt tatacgcacc aataaccgtt ccagcttcgc tgtccacgct
     3721 tcctggggta cccatgccga ttccgataaa gtcttcggct ttcatattat ataaattcaa
     3781 acgatgattg attgattcaa tgatttcagg aacgatatgc ttaccatcat ccaaaatatt
     3841 ggtatcgatg ctccattttt gctgaatctc tccttctggt gttaaaattg caaattttgc
     3901 tgttgttcca cctaaatcaa tcccaataat cttcttgtcc attacaaatt tcctttcttt
     3961 cggctttctt caattcggtg ctctcttttt aatatgcgcc acgctgtcat gtatgtatca
     4021 tgatcaatca aacggctatc atacagatta cgcaattcga tagtcattaa ttcaatgtca
     4081 tagatgcgtg cacccacata gataaagatg ccaaattttt tgaacagctg ctgtacatca
     4141 tataagctct ccatctttaa gcacttcctt catattatac aagtaaacca tattttttta
     4201 aacccagtaa aatacatatt acaaaaagaa atacaaaaac tagtgtagaa agaatccttt
     4261 ggtgtatcga atagcttgtc tgttgtttcg gtagacccaa aacattccct aacactaatc
     4321 cgccaattag cccaccgaca tgtccccaaa catctacaga ggtatccaga agaccaaaaa
     4381 caaatgtcaa cacaataaat aaaagaaaat gtctgactag ctgctggatc gctgggttat
     4441 gtttaaaatg aaaccctaag ataaacaacg caccgaacaa accgaatatc gaagtacttg
     4501 caccaccaga cagaacattc gcttcgttaa aagcaaagct tgctgtattt cccattatcc
     4561 cgctgaacat ataaatcaag aaaaaacgcc aatggccata gattgcctcg atttgctgcc
     4621 ccataaaata cagtatcagt gagttcatta cgaaatgcat cagtccaaaa tggatgaaga
     4681 ttggtgtcac taaacgccac cattcattga agtgaacaag ataaggacca tacattccgg
     4741 cataataagg gatctcaagt cgcggaaata attccatcat gaggaagacc gctgtctgta
     4801 tccctaaaaa agcataggtc cataacggtc gtcttaacca tgcacgcaat tttatttgct
     4861 gttgataatt catgaaaacc tcccatttca ttttagtcaa taaacaattg ttggatcttc
     4921 tgatcaaagc tctccggttg ccattgttca ttcaattgtt cacgaaaaac aaggctacat
     4981 gtttttttat gaaaattatt taaataccgg tcgtaataac caccgccaaa accgattcga
     5041 taaccggctc tattgtagat aatacctggt acaatgagga gatcgatcat ctccggcaaa
     5101 tagagcttct ctgattgtgg ctcaagaaca ccaaaactcg aacgtacaag aacggactgt
     5161 tcatccattt cataaaacgc catctgacgt ttcggcaaag ttttaggtac aactattttc
     5221 ttttgttctt tttctgctcg ttggataacc ggctgtgtat ccagttcaaa ttcattcgat
     5281 aaagtcgtcc ctatcacttt tgctgacttc cagtactcgc tggcgaatag ctgtttatag
     5341 attcgctgtt ctttttcctt tttcttgcca cttttggcca gatttttcag cttttcgatt
     5401 cctttccggc gtaatcttac tttcaattgg tccaaaaact cacccttcct tctctcataa
     5461 aatggtatag gaaaaaaaga aaaatagcaa tcgaactttc aaataaatgc ataaatagaa
     5521 taaaaagaag acctagaaat gattggcctt ccttttttaa tgaatttcaa ctgttcagcg
     5581 cgtgtttttg tctgataaaa agtgcataca gaaaatatgg cgcaccaaaa agggcaatca
     5641 aaattcctgt aggtacttcc ccacttggta gaatcactct agcaaccata tctgcactaa
     5701 gcagaaaaat ggaaccgaac aaaccgctca gaacaaaggt ccagttattt tctttacgta
     5761 cgactagctt cgcagcatga ggagctagta gtcctacaaa agataaacta cctgtcaccg
     5821 ctacactacc gcttgccaaa gcaacagcta acaacaaaag gattttttga cttttagcca
     5881 cgttaaggcc taatccttgc gcgcgttctt gaccaaatga tagcaataca atcctctgtg
     5941 cataaagtcc tcctagcgga attaagataa ccgcccatgg aagcaatgcc caaacataag
     6001 gccagctcgc accccagatt gatcccgcta accatgcatt cacaaatcca taattttcct
     6061 tagataattg gatcgtgcat agcaatgtta atgcagaaag gccggcatta acggcaatac
     6121 cggacaataa gattcgattc atacccaaaa agccatttcg atcataggcc gcaagataga
     6181 cacagcaagc agctaaaaca ccacctagaa aggctacaac tagcaataga aacggcagcg
     6241 aatagtgcga aaagaaacct aaataaagca tcactaaaaa tcctgctccg gcattaatgc
     6301 ccaatatact agaatctgcc aattcatttc ttgttactcc ttgcaagata taaccagaaa
     6361 cgccaagaca agctcctgct aataaactga tcagtaatcg aggaactcga aattctctaa
     6421 caatcagcgc caaagaagat gattctttcc ctatgaacgt ggcaaaaagc tgatccaggt
     6481 aaataaaagg cgtaccaatc attaaactac ctatagacag gatgacaagt acaaaaagca
     6541 agaaatagaa cgtccaatat atttttttca ccgttgttcc ctccttactt gtatcagtaa
     6601 aaacggaacg ccaatcaatg caatgactaa tccaaaaggt gtttcaaaag ggggattgat
     6661 catcctcgct gtaaaatccg ccaacataac caatccaccg ccaactaaaa aagagacagg
     6721 tagaacataa cgatagtccg ttccaaccat tccgcgaaca actggcggaa ccaacagccc
     6781 aataaatgaa acaggtccga ctaacgaaac ggcaatggct gctaacactg caactagaca
     6841 gaaaacaatc aaccgcgttc gtttaggatg acttccaaga gctaatgcat gtgtttcact
     6901 caaatgaatc aaagatattg cttttcctaa tgaaaaagaa accaaaacag caagtatata
     6961 taaaggaaac aatactgcta attgtttcca tgtgatattg gcagctccgc ctacaaacca
     7021 aaaagcaaga tcctgctgca gattaaagaa ttgggtaagt gcttggctga tagcagtaaa
     7081 aaaagaacta atagctacgc cagctaaaat cagctgtaaa ggattcatgc ccattttcga
     7141 aaactgcaaa aaaccaaata cgagcaaagt tacagcaaaa gaacctgcca atgacattag
     7201 aaacacagaa aaatcagaag ctttaggcca aaaagcaaaa ctaagggcca ttgccaacga
     7261 agcacccgcg ttgattccta gtaagcctga atctgctaaa ggatttcttg tcaccccttg
     7321 catcaatgca ccgctcaatg caaaacaact ccctacaaga aatgctcccg ccacacgagg
     7381 caaacgaata tttcgtatga tttggtgact ttgttttgat ctatcaaaat caaacaacgc
     7441 ctgccaaaaa tctgcttttt ccacagtcgc tgcgccattt gaaagagcaa caaaaaaaat
     7501 aatcaaaaga acaaaacaga ccaaaagcag gagtctattt ttcttcatag tcctcataat
     7561 tgatcctcct gctcttttgg caacaaatca taggttagaa aaactggttt atttgttctt
     7621 ggaagcgtga caatcgttgc atcaatctca aatatttttt tcatacactc ttttgtgatc
     7681 acttcttcag gtgtaccttc tgtgatcaac ttaccgtttt tcattgctaa taaataatct
     7741 gaaaaacgtg aagcatgatt aatgtcatga atcgacatca agatcgttgt ttgtttcatt
     7801 tgattgattt cagtcaataa ctgtaggatc tctaattggt gtgccggatc taaaaatgtt
     7861 gtcggttcat ccaagatcaa gatatctgtt tcctgagcaa gcgccatagc gatccagaca
     7921 cgctgggttt gtccaccaga cagcgttgcg atatcccgca tacgaaatcc agctagacct
     7981 gtagcatcca acgcccactc aattgcttca tgatcttgcg tgtccatgcc actgaacagt
     8041 ttttgataag ggtatcgtcc gtaagatact acttcttcta ctgtcatacc tgtagggtgt
     8101 tcactagttt gtgctaataa tgcaattttt tttgcaagtt cttttgaact gtatgtagag
     8161 atatcagagt tattgacaat gatctttcct gatttcacag gtagtatacg ggtaaaactt
     8221 ttcaataaag tagattttcc acttccattt ggcccaatca aactagtgat tttcccttca
     8281 ggaatcgtaa cagacatgtt ttcaatgatt tttttgtttt cgtagccggc tgcaatgtca
     8341 gctgctctta atctcgtcat ttcacccatc ctttcgctga aaatcattct caattatgac
     8401 gctgtttcaa catttcgtca agcgctactt tcatatctat agccaaatga agcggttgac
     8461 agcgttcgaa atatcaggaa taataaccaa tgagaaagat tctcaattat gttaggagga
     8521 aagaaatgaa attaaaaaca aaagtattca cattatctag catattgctg ctggctggag
     8581 tactgagcgc ttgctccccc tcttcagata aagaaacagc tacaactgcc aattcgacga
     8641 cttcatccaa tagtactcat acagtcacag atacacttgg acataaagta gagattccaa
     8701 acgcgcccaa acggatcatc ggcagttact tagaagacta tctgattgca ctaggggaaa
     8761 aacctatcgc ccagtggact gttggcagtg gttccattca agattatctg caagatgatt
     8821 taaaggatgt cccaacgatt tcttatgacc ttccttatga gaaagtgtta agctttgagc
     8881 ctgatttgct gttgatttct tcttccgcta ctgtcgaagg cgggaaatac gaacaatata
     8941 gcaaaatcgc gccaacttat gttgtaaaaa acggaacaga tgtcacatgg gaagaacaac
     9001 taaaagatgt cggcaaagct ttggcaaaag aaaaagaggc agaaaaaatc atttccgatt
     9061 atcaaaaatc catcaagaaa actcgagaag aactagcaga taaaatcgag aacaagacag
     9121 ctgcagtgtt gtgggtgacc aacaactcag cctttatggt cagcaaaaat cggtcaagtg
     9181 gaagaatcgt atacgacgat ctggaatttg gtgttcctga tctagtggaa gaggtctcca
     9241 aaaaagccac ttctgactgg tctgctgttt cctctgaaaa gctagctgaa ttagatgctg
     9301 actacatcat ccttgtgaac agtgacaaag atgctgccat gttcaatgaa ccaagctgga
     9361 agaatctaaa agcagtgaca gacaatcatg tactgcaatt cggacctgaa tctagttggc
     9421 tatataacgg acctattgcc agtcaggaaa tggtcaatga tatcaaagaa aaactaaatt
     9481 gattttaact aaaaaaagca tcttcacgat tagtcgtgag atgctttttt tcttattaac
     9541 aaactagacg catagatttt attcttcttt tgaagcaacc ctatttttta ctctttccca
     9601 tttttgaaaa cgatgggcat atagattttc ttgatccgtt atcccctcat actcctctat
     9661 aagggcgaag tctgaaaaat caatattccc aatatacgta tcgccttcaa acgtgctatc
     9721 gatcaatgtt cgccagatcc gaccagtgta aggaagaagt tcttgaaaaa tccgactacc
     9781 acccgaaaca tagatatctt ctgagatcgt ttgactatac gacaaaagtt catcgatcga
     9841 atgcattact tccgcatttt ctttcgtgcg aaaatttggt tgcgttgtca acacgatgat
     9901 atggcgataa ggaagtgaaa gatcccccat tccttcatag gtctttcttc ccatcactaa
     9961 aattcgatcc attgtatgtt cgcgaaagaa acgcatatca tttggcaatc gccatggtaa
    10021 caaaccattt ttgccgatca gaccattttt atcttgtgcc cacatggaaa taaacatatt
    10081 atccgctcct tatgatttta gatgttttga gataaaaaaa gtctcaattc tttgaccagc
    10141 aaggaattga gaactttaaa aacattattt tgtttcacgg tgcaaagtaa ctttgcgttc
    10201 gcgtgggcaa tatttttgtt tttccaaacg atcaggattg ttacgtttgt ttttgcttgt
    10261 tagatagtta cgttctttac aagaagtaca ctctaaagtg atgtttacgc gcatgttttt
    10321 ccctcccatt tttacaagaa tcctctaaga tgactctcag acttgattat cttaccatga
    10381 ttttttctag attgctagtg tttttcacga ttttgttaag taaaactact tgacgataaa
    10441 tacaacggtt ttacttctca aacaaaatct ttttcttttt gtataaatac ccttacactt
    10501 gtttatatag attatgtttt ttaataaagg gggatactaa tatgccatgg aacatgaagg
    10561 actttcctgc atcaatgaaa aacttagata aattgacaag aaaaaaagca atcgacatcg
    10621 caaatgcatt attagatgaa gggtaccctg ataaccgtgc gatcccaatt gccattgatc
    10681 aagcaaaaaa atggtatgaa gatgctagtg aatcagaaaa acgaactttc gaaaaagaaa
    10741 aaaatccaag caagacggat gaacacgata cgaatcctcg tgctggaaaa ctgcttgatt
    10801 cagatgtgat cgtagaatat gaggaagaac aatggatcgt gaaatctaaa ggggcaaaaa
    10861 aggcaagcaa ccattttgat acaaaaaaag aagcaatcga aaaaggaaag caagtagcac
    10921 aaaacaaaga atcaactttg ttcatttata aaaaagatgg gacaaaagaa aaagaaatcg
    10981 attattgaac atatgtaaag gggttgtgac gaaaatcgtg tcacaacccc cttatgttcg
    11041 aaacaatggt gttcctctgc caactccgaa ccataagtca taatatctat aaaacgggaa
    11101 gagctgtttt ttgaaattgt ttcttatcac tcagtgttaa acggtaggag aaaacctctt
    11161 ttttattgat atttgatttt catcaaacag tcacatagtc gattgcttct gcttgcggaa
    11221 tcgtttcatc tacaaagtag actttatacc atgtcaagaa actgaagatt gtccagattt
    11281 ttctttgctc ttctttacgt ccttcgtgat ggtcatctag caacttcatg attttttctt
    11341 gatcaaaaaa ttcttttgca aaatctgctt cgaataatgc tttgacttgt tcgtagccgt
    11401 gttcttcttt caaccaagct ttgattggca cagggaatcc taatttcaca cgattcgacc
    11461 attcttccgg aagatgtttg ttcgcagctt gtctgaaaat atcttttgtg ttgttttgat
    11521 tcaataaaaa cttacttggt atttgttgag ccaatttcat gacttcgata tccaataaag
    11581 gtactcggac ttccaaagaa cttgccatag aaagtttatc tgcctttaac aagatatctt
    11641 ttggcatcca ttgatgaaga tcgacatatt gcatcttatt gacttcatct ttgattcctt
    11701 ctgttttctc gtaatgaaca gtcatgattt catccacaga tggtgctgtt tgatattttg
    11761 gtgttaacac ttctgatgct tctgattctt cgaacactcg agcatgaccg ataaaatatt
    11821 cttttgcagg agcaagtgat tcatataaat ggatccgtcc atggaagttt ttcatttttc
    11881 cgattttacg tcctaaatta tagcgtgttc cttttggtaa ttttttcaat ccttgagcaa
    11941 tcacacggat gaattttgaa ttcgtatgga atccatacgt ttggtatccc gcaaacagtt
    12001 catccgcccc ttctccagat tggactaccc gcacactttg tgaagcaaga tttgctaaaa
    12061 agtacaaagg tacgcaagaa ggatttgaat caggttcatc taagtgatac tggatcaaag
    12121 ggaatgcttt gaacgacatg tctccgtcga tcactgctgc tgtattatct aagtccaata
    12181 gttcggttag ttttctggct tcaatcgctt cattataggt tttatcatca aacccgattg
    12241 agaaagaatg atctggtttc aatacagagg ttacataact ggaatcaacc ccgctggata
    12301 agaaagaacc aacttctaca tcactgaccg tatgtgcttc aatagaagct tgaacggttt
    12361 cgtcgatttt ttcgatccat tcttgtcggg aatgcgtttc ttttgtttca aaatctgcat
    12421 cccaatattg ctgaatgtct atttttccat ctttatacgt ataataatgg ccttcaggca
    12481 aacgatagac atctttgaag aatgtttcgc cgtttaatgg agaatattgg aaagtcatat
    12541 aaggtttcaa cgcttctttg ttcaattctt tgttaaaatc aggatgaggc agaaaacttt
    12601 tgatttctga accaaacata aatgttccgt tcatttctgc ataataatat ggtttgatcc
    12661 caaagtggtc tcttgcaccg aataactcat tcttttcatt atcccaaata gcaaaagcaa
    12721 acattccgcg gatcttttgc aataattctg ttccccattc ttcgtaacca tgcaaaagga
    12781 cttctgtatc tgcatgtgtt tgaaaaacgt ggccagctgc aatcaattct tctctcaaag
    12841 gctggtaatt atagatttcg ccattaaaaa taatgatctt tgtccgatct tcattgtaaa
    12901 taggctgagt accgccctct aagtcaataa tacttaatct tctgaatcct aaagcaacat
    12961 gtttatcgat atattctcct gaactattag gtccacggtg aacgattcga tccatcattg
    13021 tattaataat tgtttttttg ttgtccttgt cgttaacaaa acctacaata ccgcacattt
    13081 aactttcaca tcctaatata aaattactgt ttatttcatt catatgcatc ttatcataga
    13141 ataaatagcg gacgaatatt ttctctctta caatttcgtc acgtgaaaac aaaagatgaa
    13201 agtttctgtt tttacagaaa ataaagtccc aatatctaat ttaccatgtt ttcttttttg
    13261 aggctacctt attctcttcg tgttaaaaaa atattagtgg atgctatttc caaaaaaacc
    13321 agcaaaaaaa gacacgacct aaagatcgtg t
//
LOCUS       DAPCMT010000078        13241 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPCMT010000078 DAPCMT010000000
VERSION     DAPCMT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.079"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(12..1535)
                     /locus_tag="RD743_001818"
     CDS             complement(12..1535)
                     /locus_tag="RD743_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDU0563626.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="RD743_001819"
     CDS             1787..2968
                     /locus_tag="RD743_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDU0563627.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="RD743_001820"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="RD743_001820"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDU0563628.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="RD743_001821"
     CDS             complement(5053..7764)
                     /locus_tag="RD743_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0563629.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="RD743_001822"
     CDS             complement(7774..8475)
                     /locus_tag="RD743_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0563630.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="RD743_001823"
     CDS             8661..9260
                     /locus_tag="RD743_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDU0563631.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="RD743_001824"
     CDS             9341..10354
                     /locus_tag="RD743_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDU0563632.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="RD743_001825"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="RD743_001825"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDU0563633.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="RD743_001826"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="RD743_001826"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDU0563634.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="RD743_001827"
     CDS             11802..13175
                     /locus_tag="RD743_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0563635.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
BASE COUNT         3761 a         2628 c         2454 g         4398 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca t
//
LOCUS       DAPCMT010000079        13152 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPCMT010000079 DAPCMT010000000
VERSION     DAPCMT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.081"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..292
                     /locus_tag="RD743_001828"
     CDS             <1..292
                     /locus_tag="RD743_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDU0563636.1"
                     /translation="IITVDKEGRLTNVDKVRGRNKSIQTIVNETVKTIVAPLDQIVYV
                     AYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYGCIAIFSMGISR"
     gene            complement(421..2043)
                     /locus_tag="RD743_001829"
     CDS             complement(421..2043)
                     /locus_tag="RD743_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0563637.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2292..2813
                     /locus_tag="RD743_001830"
     CDS             2292..2813
                     /locus_tag="RD743_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDU0563638.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(2999..4729)
                     /gene="spxB"
                     /locus_tag="RD743_001831"
     CDS             complement(2999..4729)
                     /gene="spxB"
                     /locus_tag="RD743_001831"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDU0563639.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            4894..5712
                     /locus_tag="RD743_001832"
     CDS             4894..5712
                     /locus_tag="RD743_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDU0563640.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(5815..6012)
                     /locus_tag="RD743_001833"
     CDS             complement(5815..6012)
                     /locus_tag="RD743_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563641.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6181..6759
                     /locus_tag="RD743_001834"
     CDS             6181..6759
                     /locus_tag="RD743_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDU0563642.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            6854..6991
                     /locus_tag="RD743_001835"
     CDS             6854..6991
                     /locus_tag="RD743_001835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563643.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(7804..8307)
                     /locus_tag="RD743_001836"
     CDS             complement(7804..8307)
                     /locus_tag="RD743_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDU0563644.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            8666..9691
                     /locus_tag="RD743_001837"
     CDS             8666..9691
                     /locus_tag="RD743_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDU0563645.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10014..10892
                     /locus_tag="RD743_001838"
     CDS             10014..10892
                     /locus_tag="RD743_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDU0563646.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            11368..11484
                     /locus_tag="RD743_001839"
     CDS             11368..11484
                     /locus_tag="RD743_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDU0563647.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            12412..12750
                     /locus_tag="RD743_001840"
     CDS             12412..12750
                     /locus_tag="RD743_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563648.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            12844..13074
                     /locus_tag="RD743_001841"
     CDS             12844..13074
                     /locus_tag="RD743_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563649.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4170 a         2188 c         2499 g         4295 t
ORIGIN      
        1 tattattacg gtggataaag aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa
       61 caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac cacttgatca
      121 aatcgtttat gtggcttacg ctggggatct agagagcgcg gagaaggcaa aacggctttt
      181 ggaagagcag ataaagatga aagatgtgaa gctttatccc ttaggaccaa cgatcgccag
      241 tcacacaggc tatggctgta tcgcgatttt ttcgatggga atcagtcgat aaaagcagta
      301 actcattctg taaaaaagcc cagaaattga ttgtaacaaa aacagcgact cttaaaagat
      361 agatgagcat cctatctctt agaggtcgct gtttttttat tttttaagct aaataggcca
      421 ttatgcccat aactcgttca attgtttttg aatatctgca ttttccaaaa attcatcata
      481 tgtcgtatca gctcgatcga tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa
      541 tgtttggata aattgatggt catgtgacgc aaataagatt gagccagtaa atgccatcaa
      601 gccgtcattc aatgctgtga ttgattctaa gtctaaatgg ttcgttggat catctaatac
      661 aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg caacggactt tctctccacc
      721 agataaaaca ttcactggtt tcaatacttc ttcaccagaa aacaacattc ttcctaagaa
      781 gcttcttaag aaggtgttat catcttcttc tttacttgca tattgacgca accagtctaa
      841 aatcgttaga tcagtatcga attctttcgt tgtatccttt ggtaaataag aattacttgt
      901 tgtcacaccc caacgtactg ttccagtatc tggcgtaatt tcacccatga taactttgaa
      961 tagcgttgtt gttgttatat cattttctgc gacgaaagcg accttgtctt ctttgctcaa
     1021 attaaaagat atatcatcta aaattttctt tccatcaatc gttacactta cattttctac
     1081 ctgcaataag tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg gataacgtct
     1141 agaagaaggt tggatatcgt ctaaagtaat tttttcaagc atttttttac gtgaagttgc
     1201 ttgttttgat tttgatgcat tcgcactaaa gcgggcaata aattcttgga gttctttgat
     1261 ttgttcttct tttttggcat ttgcgtttgc ttgaagttta gccgctagtt gactagattc
     1321 caaccaaaaa tcataatttc ctacataaag tttgatttta ccaaaatcta gatccgccat
     1381 atgtgtacat actttgttca aaaagtgacg gtcatgggat acaacgatca cagtattttc
     1441 aaaattgatc aaaaattctt ccagccaatt gatcgattgg atatccagac cgtttgttgg
     1501 ttcgtctagc aaaagaacat caggtttacc aaatagtgtt tgtgctaata acacttttac
     1561 cttttgtcca ccagttaatt cactcatttg ctgatggtgc aattcttcag ggatgttcag
     1621 accttgcagt aaaacagctg cttctggttc agcttcccat ccattaagct cagcaaattc
     1681 tccttctaat tcagctgctc gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat
     1741 cgcatctttt tctttcagca cttcatacag acgcttgtgt cccatgatga ctgtttcaag
     1801 tacagtatag tcttcatagt cgtaatggtt ctgtttcaat acggccatcc gttcatctgg
     1861 tcccatagta acggaacctg tggaaggctg cagatcccct gataacactt taaggaacgt
     1921 tgatttacct gcaccatttg caccaatcaa gccatagcag tttccggggg taaatttaat
     1981 attcacatca tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat
     2041 caattttttt cctcactttt atcattatta gttttgacaa gtcttataga attatatagg
     2101 ggaaaggtaa aagattcaag gcaatcgaac attctttgtg tttcacgtga aacagaatga
     2161 aaaaaataaa aataaaagcg gagcgaaaat agctactata ggatttatta gtttgcaaca
     2221 gcataatttt tgacgttatt tataagaggg agtactataa aactgtaaaa gaaatgaaag
     2281 aaggattgaa aatggatgaa aaaagaacca tcgactgggg ctcattgatt ttgggaattt
     2341 tatttgtact tgtctcattg ttatcatttc aagaccctgt cggaaattta gttgcaattg
     2401 tagtagtctt tgcgattttt gcctttatta aagggatatt tgaactgttt gtacggaaca
     2461 gattaaaaga gctaacagga tataaaggaa agatgccctt aataatcggg atcatcgata
     2521 ttctagttgg cgtctttttc ttattcaata ttggtgcagg agtcgcagca ttgccttttg
     2581 tttttgcagt atggtttatt gctgattcag ttttagcgtt atttactgct gacttggcaa
     2641 gaggagtaag tgaaggatat tattggttta cgatcatcgt caatatttta ggaattttat
     2701 taggaatttt cttattgttt aatccaattt cttctgcgtt gacattaagc tttttagttg
     2761 gattctattt tatgttattt ggtatcacac atatcgtcta tgcatttaga taagtaaaat
     2821 caaacaaata aaaaagccgg cttccgatta cagttttact gttgtaattg gaagccggct
     2881 tttttatcat ggacaaatat gcacgaggtg atttttaggt gtcgagacaa atttatgcaa
     2941 aaatgtgaga agctatatac acaaatttta tcttaatact cgaagcatag cgcagttgtc
     3001 acaacctctt ttcatataat tcattcaata ctggcaaatc atggacttca tatttttctt
     3061 tgaatgcttg gatttcttct tcagaatatt ttcttggatc taaatgcaga tcttctactg
     3121 gcaatgggcg cttgttattg atcttcacat cgattactac aggacgatct gattttgcag
     3181 ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac tgtaaagcta tttgccccga
     3241 gcgcttctcc aactttaccg aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt
     3301 ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc attcgaaaag acaacattaa
     3361 taattggaag ctgatatttg acttgtgtaa tgatatcttg catgaccata gcaaacccgc
     3421 catcaccact taaggtaaat acttgttttt ctgggtagct tagttgtgcg gcaattcctc
     3481 ctggcacacc attacccata gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt
     3541 tcatattcaa taaacgaata gaatggatag tcgtgttacc gacatctgtg acgaaaatcg
     3601 catcgtcttc agcaatacga ttgatttctt tatagacagc ctctggacga atcggttctt
     3661 cctctcgatc ttcaaaagat ttcaaccaat ttacccattt ttctttattt tcttgatttg
     3721 ctaggtacca gctatcagca gggcgagcat cccctaactc gacaagtctt tgcaaagctg
     3781 tgtttgcatc accgaggatc gacaaagatg tttcatggcg tcgtccaaat tttgacgcat
     3841 cgatatcgat ttggataaat tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt
     3901 cgctaccgac aaataagatc agatcggctt ctgccaatgc ttcatttccg ggtttagtag
     3961 cgacacgtcc agcaaaacca agaaagtttt cataatcatc aggaataatt ccttttgcta
     4021 gaacggaagc gacaacagga actgaaaaat gttctgagaa ttctttgatt tgcggaaatc
     4081 cgtttcgcgt tccttgacca acataaagaa ctggtttttt tgctttttca agataaggca
     4141 aagcagctag caaatctttt tcttctggta atatcacact tgttttgtga gtgtgagcgg
     4201 tggagaaagg ttgttcgtca atttcttcaa aaccaagatc aacagggata gtcacgatag
     4261 ccacgccttt atgctcatag gcggctttaa tggcttcatc tactacatgc ggaagacttt
     4321 gtggtgtcat gactgtacga ttgtatacgc taacatctgc gaaaatagga ttttcattta
     4381 gttcctggaa agagttatag ttcatggatg tagaagcaac ttgtcctaag aacgctacta
     4441 gaggcacatg gtccatctgt gcatcataca aaccattgat caaatgtgtc gcaccaggtc
     4501 ctgcagagcc gaagacagcg ccgactttcc ccgttaattt tgcatctgca gcggctgcga
     4561 gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat ttcggttttt tcttgatata
     4621 gcgcatccat aattgaattg aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc
     4681 agctttcaag aacttttacc attgcgattc ctgcattgat ttttgacatg gtgtttcact
     4741 cctttagcaa tgatgataca taaatttttt gtatctagag tatagaactt tttatcgaaa
     4801 atcccaacta attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat atgctagagt
     4861 agtacataga tagattcaag agaggggaat ggcatggttg aatggctatt gagacttgaa
     4921 ccttggcagc aagcacttgc tgggacagct tttacttatt ttatgacagc tcttggtgcc
     4981 gggttggttt ttttctttaa agaaatcaaa aaagaagtgc taaacttgat gttaggattt
     5041 gcttctggtg tcatgattgc tgcaagtttt tggtctttgt tagatccggc tattacaaaa
     5101 gcagaagaaa atggagatat tgcttggcta gttgtaagta ttgggtttgg tttaggcggt
     5161 gtgtttttat atatggcaga taaaacactg ccccatatgc attttggacc acaacatgaa
     5221 gcagaaggat tgcctaccca tttaaaacgg acgatcttgc ttgttttttc tattacattg
     5281 cataacattc ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc aactgcggat
     5341 aatccgacag ccgctatatt agccgctgtt tcggtggcgc taggtattgg tatccaaaac
     5401 ttcccagaag gagcagctgt atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag
     5461 gcctttgttt acggacaagc ttctggaatc gttgaaccaa ttgctggagt gattggagcg
     5521 ctactagtaa cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc cgcaggagca
     5581 atgatttatg tcgtggtgga agaattgatc cctgaagcac agcagacatt gtccagcaaa
     5641 cgtcattttg cagtttttgg cgtcatgagt ggatttatca ttatgatgat tttggatgtg
     5701 gcattaggat aaataaaaaa aatatatatg atatatcaaa tagggccgcg aacttatatc
     5761 gcgaccctat ttttttagta agttatatca taatgaagtg aatggtatca tacattaata
     5821 atagtttttt ctatgtgtat ttcctttgat ccagcgaaca agccagatga taagtgcgat
     5881 tgggattaga actttgaccg caaaccataa aagcccgctt aggacactga caaaaagact
     5941 gatgacgaag ctagctgcaa ttagcactaa aagaaccatg aagatattta agaacatatt
     6001 tttttgacgc atacaaaacc ctcctcttgt tcattcttta ctatagaaaa agtataacag
     6061 ggattgttta aaaacacctc caactgaaga ctgattttta cttcatctac ttaaatgatc
     6121 aaacaattgc tttttctctg gtttatcatc taaaatagag cggaaaagga ggagaatatc
     6181 atgcctaaaa atcgtcttga agcatttaca gatgctgtaa tcgcgattat catgactatt
     6241 ttagttttag aacttcatga acctaacgga gacacgtggg aggcgctcgc tgcattaggt
     6301 taccgatttt ttatctatat tgtcagtttt atcattttag cgatttattg gaacaatcat
     6361 catcatttat ttcaagttgt acataagata aatggacggg tcttatgggc gaacagtttc
     6421 tttgtttttg cgctttcgct ttttccgttt gcaacttcat ggatcagtga gtacatcaat
     6481 agcctggctc cagaaatgac ttatggtttt gttaccttga ccgctaatat tgcgtattat
     6541 atcttggcaa aagaacttgt caaagcaaat aaaggccacg agaaagcatt gaaggcgatg
     6601 ttgaataatt acaagaaatc gtatctatcg attggtttga atgtgttagg aattgtctta
     6661 gggtatttag tagcgccagt agccgtgctg atcgcaaaca tcgtgattct agctgcttgg
     6721 atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt
     6781 aagagaaaaa tatagaaaaa ataaataaac gatcaaatat ccgagtgata ctagtattca
     6841 aagagggatg cagatggatc aaaaaagaat tcatgtagga ctgattacta gttttgtcac
     6901 gatgcttatt tatttggatc aaggtcattt gacttggaca gtagttggtg tcgtaggatt
     6961 tttgacatta ttcacagcaa taaaaaaata aacatcttta tgtaatctga tgttctatct
     7021 atttaagaaa agcatgttta cagcagtatg attctgttgt aaacgtgttt ttatttgtgg
     7081 aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag ttagtttttc cacagtggat
     7141 aacttcgtaa gagataaaaa taaaaaatta gtcaaagaag ggcgtataga tttcttttga
     7201 taggagatat acaacagaaa tgaataaata ttaacgtgtt ttcaaacagt ataaaacggt
     7261 gtggtttttg gaataaaaaa ataacatatc tcattttacg cttttgttat tttatactta
     7321 aaaaataacg acacagaaaa taccgtgtct cagatttctc tactacttct aaaaactact
     7381 gtttttttcc tttgaaatgc cttcaaaaag taggatcagg cagaatatac tggcagaatc
     7441 aaggagaagg gagcctaaat aaggagaaaa accatagaaa atataaagta attacttata
     7501 aaacaagctt tttatagaaa aataatatac ctgagtaggg tataaattaa gacggaattc
     7561 ctttctagat tgagatattg actaggaaga atgttaagat tttaagcctt taagctaaaa
     7621 aaaaaccctt tttgttaaat aaggggaata tagcctgtca gtttcaaact gcaagtcgtc
     7681 ctgctgcaat aatatagtag attagagatg aaaatagatt tttttaaacc aatgactgaa
     7741 taaaaagacc gtcatagata cacattggaa tgtgtctatt acggtctttt tttacgaacg
     7801 attttattga agacggacag ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa
     7861 taaaatgtat cccaaaataa tggctagacg gtctaactta tttgtcgata gatgtttgtt
     7921 tagaagttga taggcccata aacctatcag tcctcctgtt gtattagtta tcaaatcagt
     7981 gatgtccgct acgccaatac cgaataggaa ttgaatcaat tctgccaaga agcttacaag
     8041 gaaaataatc cctaattttt tagaaaaaga aagtctcttg aaattgacat tcaaaagcac
     8101 cccaaaagga aaaaagacaa tcaagttata agtggtctca cgtaataccg tcgtattttc
     8161 aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg taagaataag tgagaaattg
     8221 tggaagatta aatgatagct tgaataaaat caaccataat agaaagaata aataaatccc
     8281 taataaaaat tttgatacat accgcataga catctcctcc atttaaaaag tataccatcg
     8341 aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg accaacgtag ttatcgtacg
     8401 aaaattagaa gctttttttt gttgggaatc cggtttcttt aacaaagcta agatagcgag
     8461 ttgcttggaa aagttgctat cttttttctg tcaaaacgac tcggaatgtt cgttttttgt
     8521 tctaatttct ccaagttgtt tactttttga tactaagttt atgaaaaatc aagaaatagt
     8581 tgttatacta ggtaatgctg acaaacggga ttggcatttc atcttggttc agcttttttt
     8641 ccaattacta ggaaggaggg atagattgtt caagacttat tttcgtttca ataaaataca
     8701 agataatcct tttcgtatca tgaatctgat gctacttatc tttattgtat tatggttagg
     8761 ttacataaat catgattttt tgatttcaag tttttcatca ttagggatct ttactttttt
     8821 ctattatcaa aacataccga tgaaatattt gctgaaacgt atgtttctga tcggttgcgg
     8881 actgttggtt gctttcgttt tggggatctt atcgacttat gttttatggc ttgaaccatt
     8941 tgccgtagct ttggttgctt tttccagcag attcgtgttg cgattattcc atatttctaa
     9001 accaggtggc ttgttttttg cgatgctttc tgccatggga acgagcatgc agctgccaat
     9061 cgctcaatta ccgatcgtct cactttattt ttttatgggt gtcgtttttg ccctgattgc
     9121 ggcagtgatt acaaaattac tggattcacg gcctgagcag acaattgaaa aagcaacttt
     9181 aaaagaaaga tttcacgaag agcctttagt gatcattgat tctgttttct acagcgctgc
     9241 cttatttctt tccgtttatg tcagtcacgg cttgaatctg cataatcctt attggttgac
     9301 cttgtcatgt gcatcgattt tgctagcaga aaacttggat gcgatgaagc atcgacaagt
     9361 ccaatatctg attggatcga tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc
     9421 gtttacgcag ttgcagacca tttttctgat cacttttcta tatggaatcg cacaatttct
     9481 agttgctaga aattatgcag ttgctaatat ttttcttaat ccgatggcgt tgatgctatc
     9541 tacacttatt cgtggagcat acttgatttc tttgatcgaa tatcgttttt tagggatcgt
     9601 cataggaagt ttcatcggtt taggtgttgc gtgggtaatg acggttggtt tgcaacatta
     9661 tttaacggtt gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc aaaagaggtt
     9721 atgacaagat ttttgtcaca acctctaatt caaggtgttc aaaagccaga ttctcagcat
     9781 taagtcaaat tcacacaaaa atgtggaaag gaactttcaa aaaaatttta tatttattct
     9841 atcaaaaaat tttgtttatg atggtaactg aataaacttt cctgagatgg tcaatgcttt
     9901 ccttgaaagt cttacagata ttctttcttc atagaaagtt taagttttat agttagtgta
     9961 caataaaagt atgtttatac catatggtgt ccggataatc agtcatttaa acaatgaggt
    10021 tcaaagatat gaaaagggga aatttgatga agaaaataag caatgaaatc caaattctgg
    10081 cagaaacttt aactcttccc aaagtcgtta tagagcaaat gattgtacaa gcaaaaaaca
    10141 tccttgatga ggtgtttgag caatatagtc atgaactaat ggatcctaat aaagcaaagg
    10201 cgactgcaaa gaaaatagac aaagtctatg aagaggaacc ttttgcagca ttagctattt
    10261 atctatatgc agcttctcaa agttggaaaa aaatttatca accattgaag attccaagaa
    10321 ctatctattt ggcaacaatg aatgctttta ctagatttat tcatgaacat ttccaagtaa
    10381 caggaagcta tcgctttgac cgtggttttt ggatttggca ttacactagt ggcttgatct
    10441 ttcggattgg tgaattggag tacgaaatga catattttcc gaaagggtac acggatctaa
    10501 gattagaagg aaagcagtgc ctttctcttc atattccttc cgatgcagat ttatctcaga
    10561 ataagataag agatagttac gaaagtgcca ttcgtttttt tgaactttat tttcctgatt
    10621 accattacca agtgatgtac acagatactt ggttactatc acccaatctt actaaatggt
    10681 tgaaaaaaga gtcaaaaatc tgtctttttg cagccgatta tcgattatta tcagttgatg
    10741 aacaggacga tagtggagtc ccttggatct ttggacgagt agatgcccaa atacatgatt
    10801 atccagaaag tacgagttta cagcgacaag caaaagagca attgcttgca ggcgagcaca
    10861 ttggctctgg cttaggtatt cttacttcat aagtttagta cttcgaaata gtttaaaaga
    10921 gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
    10981 agcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc
    11041 gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttagtgtt
    11101 ctatcgaaca cacagtacgt tcaataggta aactagctat aacaatagat attaaagtta
    11161 acggacaaga gggccttttt taggtagcta aaacgtaata atgaaaattt atttccagag
    11221 ataggagtca atcagctagt ctgatcgact gttttaacaa gttgactttc attttaactt
    11281 ttgataccat ttaaaacaca aatcgtaatt gtttcgattt gtgtttttta gcatgactac
    11341 aatcaaagaa gaaagtgggt cgtccatatg gaaaaaacag atatctcaag tgcataccgc
    11401 cgcttgaaaa gcccaaatat caaaacacga aaacgtgcgc tgaagatcat caaagatgtg
    11461 aaacggaata gtgggaaaag gtaagaagaa ggaaacttaa ttaaaaaaat agccgaaaaa
    11521 cgtttttcgg ctattttcaa taatcggttt ttgtgtaagg tttccgaaag aactgctgtt
    11581 gattttccat ttttaacata tgataaattg gtagaatctt tcttttcaaa ttgataccat
    11641 tccctaattc cagttatata gttaactaca gaaccacaaa gcacgtacgt ttataaaagt
    11701 attatctgta gttatttttc aattcccttt caacctctta ttttgaccag tagtattgat
    11761 cactttacct tttaacacct ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg
    11821 taactacata atcaaaggtt ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc
    11881 aatacggttg caaataacca ttatcgttta ataagcagtt acgaaagctc ccaatatctc
    11941 taacgttcag ctcactccat ttcttttctt caaaaaaatc agtgattcgt atctctttct
    12001 atttgtcgaa aaagttaact ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa
    12061 agcagttact attgctagaa taacatcaag gaataataag ttttggtttt cagataatag
    12121 tcgataagtt gtgtattgga attttagata cagtaataaa gtatttttca tgaaattttg
    12181 tgagaatcac tattacaagt atctttaata aaattaatgg ttaaggagga acttaaagta
    12241 gaatgtaaat gaatagttgt agtcagtcga ttgtgatact agactcaaag ttgcaggaat
    12301 aagtttttca atttgtgatt ggaagataaa aaatgaaaaa gaagggcttt tttttatact
    12361 ttgttgattg tagtgtttat tttccctctg tttagccaag taacattttc tatgactcaa
    12421 ccagtttcaa gtgaggaaca tgataacctt tttcgtgcta cagaagagtt taagaaatat
    12481 caggaaaata ttgttccgga gactccatta gctagtgagc caattactga tattgatgga
    12541 aaccaaatag gatggtatat acagtacgaa gttatggtgg taaataatga ctcaatagta
    12601 aaaaatacag taggcttaga gctaacaccg attgaaaagg tgagttcatt gttaacgtat
    12661 atttatactg atgaaagtga cgagactgag atcattttaa cggattactc aaaaattgct
    12721 actgataaaa agtatatatt gtggatgtag acactagtga aatactttat gatatagaaa
    12781 ttgatgaaaa tttactagat gtagcacaag aaatcgatca tcaaaagcag gaagctattc
    12841 gatatgctgg tagaaaaggg aaaagaaaat atttattacg tgaatgtagc aaaagttaga
    12901 gaagatgaaa atgagtggaa agaattcaag agtcgctact caattaactc gactccgaca
    12961 tttacagttt acagagaagg atcgatagaa aaaactgttt tttggactaa agaaagtgga
    13021 atgtctttag ctgaagtaga agagtttttg gattatgtta gtatgcaaca gtaagggagt
    13081 tcttattatg aaaaattttg acacatatta tgttattgtt ttttcgatat ctttgctgtt
    13141 aatattacta ac
//
LOCUS       DAPCMT010000080        13130 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPCMT010000080 DAPCMT010000000
VERSION     DAPCMT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.082"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            19..1758
                     /gene="yfcC"
                     /locus_tag="RD743_001842"
     CDS             19..1758
                     /gene="yfcC"
                     /locus_tag="RD743_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDU0563650.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1915..3099
                     /gene="iadA"
                     /locus_tag="RD743_001843"
     CDS             1915..3099
                     /gene="iadA"
                     /locus_tag="RD743_001843"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDU0563651.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3210..3413
                     /locus_tag="RD743_001844"
     CDS             3210..3413
                     /locus_tag="RD743_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDU0563652.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3514..4176)
                     /locus_tag="RD743_001845"
     CDS             complement(3514..4176)
                     /locus_tag="RD743_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0563653.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4189..5691)
                     /locus_tag="RD743_001846"
     CDS             complement(4189..5691)
                     /locus_tag="RD743_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0563654.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6239..7918
                     /locus_tag="RD743_001847"
     CDS             6239..7918
                     /locus_tag="RD743_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDU0563655.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8045..8524
                     /locus_tag="RD743_001848"
     CDS             8045..8524
                     /locus_tag="RD743_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDU0563656.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8743..9423
                     /locus_tag="RD743_001849"
     CDS             8743..9423
                     /locus_tag="RD743_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDU0563657.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9431..10762
                     /locus_tag="RD743_001850"
     CDS             9431..10762
                     /locus_tag="RD743_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDU0563658.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10777..11739
                     /locus_tag="RD743_001851"
     CDS             10777..11739
                     /locus_tag="RD743_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDU0563659.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11850..12188
                     /locus_tag="RD743_001852"
     CDS             11850..12188
                     /locus_tag="RD743_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDU0563660.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12267..12974
                     /locus_tag="RD743_001853"
     CDS             12267..12974
                     /locus_tag="RD743_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDU0563661.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4165 a         2325 c         2894 g         3746 t
ORIGIN      
        1 agaaaggaag aaataaattt gaagaaaaca aattcttcag gaaaaccgaa acgcgacttc
       61 actaaattat cgacacccca tacgtatgtc attatttttg gtgtagtcat ctttgcatgg
      121 atactgactt ttgtcgttcc agcaggaaag ttcagcacgc aagatattga atacaaagat
      181 gcaaatggcg aaacaagtac tcgtactgtt ttacgtcaag attccttccg ttatgcttac
      241 gagctggata agtcgtatgt gtttgatcaa ttagaagaac tgcaagatca tccagcagaa
      301 cgtgaaaaac tggatgttcc ggaaaaagga ttggaaaaag tcattgctga cggagaaaaa
      361 aatcttacgc aggaaaaact agatgaaatc tccttgacag atgatgtgct ttacgatcag
      421 tacggcgaaa atatctatga tacttctaaa aaattgcata aaacagccaa aatatgggga
      481 acagatgatt ttggcggctt tggtttctta aattttgttt ttgaaggact agtatctgga
      541 gataagtatg gatctgctgt agggatcgct gcattgatcc ttgttgttgg aggtgctttt
      601 ggtattatta tgcgtaccgg agcgatcgac gcaggtatct atgcgtttat cagtaaaact
      661 aagggactgg agcgtttagc tttaccgcta ttattctttg ctttttcatt cggtggcgct
      721 acattcggga tggcagaaga ggtaattcca ttctcaatgg tgatggttcc atttgtcatt
      781 gcgttaggat atgactcgat cgtagcagta accgtgactt atgtcgcatc tcaagtcgga
      841 aatgcgacct cttggatgag cccgtttagc gtagcggtgg ctcaagggat tgctggaatc
      901 cccgtgttgt ctggtgcgac attccgattg attatgtggg tagttgttac tgcattagcc
      961 gctggttata tgatgatata tgcagaaaaa atccgtaaaa aaccagaaag ttcgttgact
     1021 tacaaatcag atgattattt ccgttctcat atcaaaaaga catctgatga aaataaaaaa
     1081 ttcatgcttg ggcataaatt gatcttatta gagatgttag ttgtccttgt ctggatcgtt
     1141 tggggcgtga ctcaaaaagg ctattacatt ccagaaatcg cttctcaatt ctttgtgatg
     1201 ggattagttg ctggaatcat tgctgttctt ttcaaattag atggaatgaa agtcaatgac
     1261 atcgcttctt ctttccaatc tggtgcagct gatttagctg gtacagcaat cgtagtaggt
     1321 atggccaaag ggattcttct tgtcttggga ggatctgatg catctgttcc ttcagctttg
     1381 aatacgattt tgcatgggat cgggacggcg ttgaccggtg ttccagctgt cattggtgct
     1441 tgggcgatgt acatcttcca aagtttgttc aacttggttg ttacatcaaa ttcaggacaa
     1501 gcagctttga ctatgccaat catggctcca ctggcagatt tagtaggtgt atccagacaa
     1561 gtagcagttc ttgcttatca gcttggttca ggttttatgg acgcttttac acccgtatca
     1621 gccagcttga ttggtgtgct aggtgttgca cggatcgaat gggcaaaatg ggcaagattc
     1681 caaatcaaga tgcaaggatt cctgtttgtt ttaggaacta ttttcatcat gattgccatt
     1741 gcaataggtc tgcagtagta ctggagacct gtaaataaaa aagtaatcca aacagtagat
     1801 aaggactcca agaaccgtca ttttacggta aaatcaaagg agtaaagatc ctatcgtttg
     1861 gattattttt taagatccta tcatcagtta tacttaaaga aagaggaaaa atgtatgaaa
     1921 atcattcgac aaattgaagt gtttgcacca gattacttag gaaaaatgga tgtactgatt
     1981 gccggtggga agattttggc tgtagaagaa cagctagaag gcggctacga aggagtggaa
     2041 gtagaagaac tttcaggtga gggaaaagta ctaaccccgg gtttcattga ctgtcatttc
     2101 catattttag gtggaggagg agaaggcggt tatcaaaatc gaacgccaga agtcacattg
     2161 agccaattga ctacagctgg tgtaacgaca gttgtcggtt gtttgggaac agacggagaa
     2221 ggtagagata tgactgcgtt gatcagcaaa gcgaaaggtc tggaagcaga aggaatctcc
     2281 acttatgtat acgaaggttc gtaccgattg ccggtaaaaa cagtaacaga ttctatcatc
     2341 aaagactttt tgacaattga taaaattatc ggaatcggag aaatcgcagt atccgatcac
     2401 cgttcttctc agccaagttt tgaagagttc gctcgtgcag tagcagatgc tcgtgtagga
     2461 ggcatgttat ccggaaaagc tgggatcatc aatgtccact taggcggagg aaaaagaaag
     2521 ctagagctat tgacacgaat cgtagaagaa acagaaattc cgatcacaca atttttgcct
     2581 actcatgcaa atcgcacacc agaattattt gaagcttgcg tggcatttgc caaacgtggt
     2641 ggcacgattg acttcactgc tagcgaagat cctgattttt gggagaagac agatggtgaa
     2701 gttcgcttca gcaaagcact gaaacgattg atcgaggaag atgtttcttt agataatttc
     2761 acgatgacct cagatggaca aggaagcttg ccttattttg atgaaaacaa tcatttccta
     2821 gggttaggag taggaagtgc caaggcattg ctagtaggga tcaaagaagc tgtgcaaaaa
     2881 gaaagtatcc ctttggaaat cgccttacga gctataacca gtaatccagc acgtatattg
     2941 aaattggata aaaaaggaaa aatcgaaatt ggtgctgatg cagatctgtg tatcttggat
     3001 aaagaaacgc ttgatattga tacagttatc gctaaaggag aaataatggt ccaagaaaaa
     3061 gaagtcaaag tatgggggac tttcgaaaaa agtttttaat caacagctct aactatcttt
     3121 tttagaaaac gagcgttctg tttgtctgta tctgttgttg atgttaccct gaaggtagat
     3181 caataaacaa actaagagga ggaagcacaa tggcagattt aaaaggacgt tctaaagata
     3241 tgaaagataa agtagtcggt gaaggaaaag aagcatacgg caaagcaaca gacgacaaga
     3301 gcaaagaaac agaagggaaa gcacaatcac taggtgctga catcaaagaa aaagctcgaa
     3361 atttaggcga cgaagtcaaa gacaagtttt ctcgaaaaga cgaaaaaaaa taaaaaccac
     3421 ttattactaa ttgaatcaag caaataaaaa atccgaacca tatctgcgag acagatatgg
     3481 ttcggatttt ttatttgatc attatcctgt tttttaaact tcgatttttt gaaatttttt
     3541 attttttagt tcaaagactt catcacagct ttttgctact tcaatctcat gagtcacgat
     3601 gataatacag cgattttgtt catgggcgat ttcttggaaa agtttgacga tatcttgtga
     3661 agttgtttca tctagatttc cagtaggctc atccgctacg attagttgat gttcacagca
     3721 gatagcacga acgatagcga ctcgctgctg ctgacctccc gaaagtttgg tcacctgttt
     3781 ttttgctaat atttcatcaa ttcctacacg agccagtcga tctaaagcaa cttgtcgata
     3841 attctcttgc ttggtcttag tgatttccat tgctgctatg acgttgtcta gagccgataa
     3901 ataaggaagc aagttgtaag cttggaaaat aatcgagaca tcatttttac gataacttct
     3961 taacccgatt ttgttcagtg aacgatcttg atagtatacc tttccttctt ttggtgcatc
     4021 tagtccagaa ataagcgaaa gaaaagtcgt ttttccagaa ccacttgctc ctaaaatagc
     4081 gtacattttt ccagcttcga aattcagttc tacatcttta taaagcgcat cttgcggatc
     4141 ttgataccaa aatcccaatt gttctgtccg taacatcaaa ttcctccttt agtttaatag
     4201 gatttttctt gggtttaatc ttaatatccc tatagaagct aaaaagacag aaaacagact
     4261 gattccaaat gctactccgg ctaatagtcc caattgtgca ggtttgacat tgatttccaa
     4321 ttcgctgact tgatcagaaa cagcaaatgg attattcgtg gttccttgtt gaccatcgcg
     4381 ttgaccgcct tgttcttgtc tgttacctgg catttgtcca ccgccaggag cttgctggtt
     4441 ttgtgtcgtg gtttctgtct gttggtcaag tagctgctgt cccacggcat tcgcgacgac
     4501 gtttccgcta agcgaagcaa ctcctaatgc aagaatcata caaatagcca cttcggtaaa
     4561 caattggaga atgattttac ttctggattc tcctaaagat agcaagacgc ctaactcatg
     4621 ccgacgctcc cgaatggtaa tcataatgat cagagtaagt atgataatac cggctacagc
     4681 cactagtaaa acaacatttt tagaaaaact tgccacgttg tttaaaggtt ccaacattga
     4741 ctgatacatg gaatcatttg attgtaagct aaatgtatct gtgtcgatca gcttctcagc
     4801 ttctttgaga aactcatcca ttttatctgg atcagataga gtgtaaatag cagaatctat
     4861 cgtgttatcg tcagatgtcc catttagttc atttgcaaaa gtgtaagaag tgtaaagtgt
     4921 attcgacgga ttcatgaagt tgaatttcat acccatactg ccgcttgttt cgctggattc
     4981 ataaattcct ttgatcgtca tttcataagt ggtatcttca tcttcagtac tcgtgattgt
     5041 gaaagtatct ccgactgcca ggtcatttgt ttctgctaga gtagaatcaa tgactacatt
     5101 gtttgtgcct ttgtcgctgt catctattcc ttcaccatcc gtgatttttg ctgttccatc
     5161 agaaaaatta ctattttgtg cagttgctga cacaccactg atttgaaagt ctgcttgagt
     5221 catttgcgga ttcccgccca ttcctccagc aggttcaccg cctgcatcag tagtttctga
     5281 actgtcttca cttgaatcag aactagtgat ggcagttatt ccatcttttg ccatagctga
     5341 ggcggaactt tcaaaggaat aactctttac attatctaat tcagctattt tttccgcgtc
     5401 cgttaaggaa actggtgtaa gagaaaaact gcctggatct ggacgttctt cagtggtaga
     5461 accgcttgaa gaagtttgtt cctgtttttt aaatgaagct tctcgattgg cacttaaagt
     5521 cactgttgct ccaacttctt tttgtgcttg attcgctgct tcttctgcag cgcttcgaat
     5581 cgtgaggcca gctagcacaa aaataagaat ggctgtgaac acggcaaaca ataacaatga
     5641 acgtccccat tttacttttg tacttttcca tgcacgtttc ataaaattca tattttgcat
     5701 ccctcctagt agatgcattt gataatagac aacctatctt aaagctagct taaatcttat
     5761 gctgtttgca caccatcaac atgaggttct gtatatataa aaagtcacta tagttattgc
     5821 aaaaaatgtg gtaaaatgaa aaaagtatgt ttcaaaccga cgaagaagaa atgaaagagg
     5881 aatagcaaaa gaaatcctag atgctacaga gggattttta cgtgcatagt tgttctaaca
     5941 attgctgata ctagcgattt agtttatttg ttgtataata atggggaaca gctgaatact
     6001 tatataagta ttctggctat agcgtttcgc aaaagatcca ttttgttaat gaagtatatc
     6061 caaaataaaa tgcccatcca actgtcagaa cgcggatgtg ctggtaattt tcctgctgat
     6121 tctccataag ttgagaaata gtgattttgc atatgcttca ttggaatggc tcatatattt
     6181 agataaagaa gaaaatcgac atctttaaac ttggatgtca taagaagagg tgaagtttgt
     6241 gagtaccatt aaaattatcc cattaggcgg cgttcgtgaa aatggaaaaa acatgtatgt
     6301 cgcagaagtg ggcgaggata tttttatttt agattgtggg ctaaaatatc ctgaaaatga
     6361 gttgttgggg atcgacgtcg taatccctga ttttacgtat ttagaagaaa acattgatcg
     6421 tgtagctggt gtctttttaa cacatggaca tgcagatgcg atcggggcac ttccttactt
     6481 gttgtcaaaa atttctgtac cagtattcgg gacagagttg acgatcgagt tagcaaaatt
     6541 gaatgtcagt cgtaatgaag cagccaaagg atttaaggat ttccatgtgg tagatgagca
     6601 tacagaaata gactttggtg aaacagtagt gagctttttc cgtaccactc atacgatacc
     6661 agattcagtg gggatcaatc tgaaaacaaa agaaggtagt atcgtttata cgggagattt
     6721 taaatttgac caaacggcta tcccaatgta tcaaacagat tatgcacgat tggcggaaat
     6781 cggcaaagaa ggagtcttag ccttactaag tgaatcggca aatgcagaaa atcctcagcc
     6841 tgtagcttct gaagcccaaa tagcagatga agtatttgat acgatcaaat attgggaagg
     6901 gcgtatcatc gtcgcttgtg tagcaagtaa tttgcaacga gtgcaacaag tattggatgc
     6961 agcagaaaaa gcacaacgaa aagttgtctt gactggtcaa gatttcgaac gaattattcg
     7021 cacagcgatg cgtttagaaa aacttcaatt accaagtgaa gatctgcttg ttacgttgaa
     7081 agacatgaaa aactatgatc cagaagaact gatcatttta gaaactggcc gaatgggtga
     7141 acccatcaag tctctgcaaa aaatggcaaa cggtacacac cgtaatgtca agattgaaga
     7201 aggagatttg gtttatatca ctactacgcc aagcatcgcg atggaaacca tcgtggcgaa
     7261 aacggaagat atcatttatc gagctggtgg aacagtgaag ttgatctctg agaatatgcg
     7321 cgtttctggc catgccaatc ctaatgactt gcaattaatg atcaatttga tgaaaccaac
     7381 atattttgtg ccagttcaag gagagtatcg tgaattagct gctcatgcag atttggctca
     7441 tgcagtaggg atgccataca aaaatatcta tattactgga cgcggagacg tgctggaata
     7501 taagaacaag cgtatgagtg ttgcaggaac tacaagtgca gaaaatgtaa tgattgatgg
     7561 attaggcata ggtgatatcg gaaacattgt tttacgtgat cgaaaaatct tgtcagaaga
     7621 cgggattttc gtagcggttg tcactatcag tcggcgagaa aagaaaatca tttcaaaacc
     7681 tcaaattact tcacggggat tcgtttacgt aaaagctagc cgtgatctga tgaaagaaag
     7741 cagcaatatg attgaaacaa tcgttgagaa acatttagaa agcaatgatt ttgagtggag
     7801 caaattaaag caagacatcc gagatcaatt aggccgttac ttatttgaac aaacaaaacg
     7861 ccgcccagtg atcttgccag tcattatgga agctactcaa cgtcgaaata aaaaataaac
     7921 aaaaagagta agatgaacct ctctatagag tgctcgtctt gctctttttt tgcgaaaaac
     7981 aaataatttt tgaatacagc atccctcgct ttataataaa cgatagaata aagggagtga
     8041 atcaatgatg agtcaaacaa aagtcgaacg ttatttagaa gaacaaaaga tcagctatca
     8101 accacttgat tttagtcatg tggtagaaga atctttcaca gaagaactac aaaaaagagg
     8161 gattgatcca gctttgatat gtaaaactct cgtagtgaaa ggaaacaaaa ctggggtgat
     8221 catcgcattg attcctttga acgatcactt agattacaaa aaaacgagac aagtgacgaa
     8281 agatcgtaaa gttggttttc ctggaatgga ttttgtatta atgcatacag gatatcccca
     8341 cggagcaaac acccctattg gaataaaact cgcacatcct gattattgtt ttttagcaga
     8401 cagttcttta aaagaaaaag aagaagtaat tgtgtcaagc ggtgaaatcg ggagaagcct
     8461 tcggataaaa gtagccgatt tagaaaagat cgtccatcct atttacgctg atttaattag
     8521 ttaagggacg agaagagaag ctaagcagag cagagacctc aaacttgaga ttatatctgc
     8581 ttggtacttg gtgcatcctg tattatctgt tacaattgct ttgtacatac tgtactatca
     8641 atccaatgaa aatatattta gcagctatat tttttcattg atgtgcaagt gatgaaatcg
     8701 tttttaataa aaaagataca gagaaaacgc gaggtgtaaa atatgaaaat tacgtatcat
     8761 ggacattcct gtttaacaat tgaaatggaa aatggccaac gcgtgatgat tgacccgttt
     8821 attacaggaa atccattgac agacttaact gcagaagaag taaagacaga ttggttgctg
     8881 attactcatg gtcatagtga ccacatagga gatatgattc ctattgcaaa agaaaatcaa
     8941 gccacaatta tcagtattgt tgagattgct gattatgcga acagcagagg agtagattca
     9001 ttcggtatga atatcggtgg aaaacatgcg tttccattcg gcgtggtaaa attcgtacat
     9061 gcacagcata gttcaagcta tgaagtagac ggtatcgttc agtatatggg cgagcctagc
     9121 ggaattatta ttcaagcaga aggaaaaacg atttatcatg caggggatac tgcttatttc
     9181 agtgatctag gattgcttgc agaagaattc gatattgatg tcgctttctt gccgattggc
     9241 gataactata cgatggggcc agaagatgca gcaaaggctg cgcaaacaat tcgtgccaag
     9301 aaagtagttc caatccatta caatactttc cctgtcattg aacaagatcc cgaatctttt
     9361 gtagagttac tggaagatag tgaaggaaaa ataatgagcg tctcggaaac gctggaagtg
     9421 taggcagaaa atggcgacaa aacatgattt gattttagaa tatattgaaa gtttacctgt
     9481 tggcaatcga atttctgtaa gaagcatcgc aaaagaattg aatgtcagtg aaggaacagc
     9541 gtaccgtgca atcaaagatg cagaaaatgt cggactagtt tcaacaattc aacgagtagg
     9601 gaccatccgt atcgaacgca aattaaaaaa acatattgaa cgtttgacat ttggcgaggt
     9661 cgtcaggatc attgaagggg acgttttagg cggggcggca ggattagaca aggtcttgaa
     9721 taaattcgtc atcggcgcga tgactaaaca tgccatggaa cgctatatta cgccaggatc
     9781 actaatgatc gtcggcaacc gggcggaagt ccaaaaatta gcattggaag atggcgccgc
     9841 agtattgatc acaggaggat tcaatacgac tgctgagatt gcaaagctag ctgatgatct
     9901 tgaaatgccg atcttacgca cctcttatga tacatttaca gtcgcaacca tgatcaaccg
     9961 agcgttaagc gaccaattga tcaaaaaaga tatcatgctt gtcagtgata tctatatgcc
    10021 agctgaaaag actcattatt tgcagcagac agatacgatt tctgattatc agcgtttgtc
    10081 agaagaaacg cagcattcgc gttttccagt cgtcaaccgc catcatcgtt tgatggggat
    10141 tgttacggca aaagatgttt tagggaaatc gccaaaccag atcatcgatc gggtgatgac
    10201 gaaagaacca atcagtgtaa agaaaacaat gagtattgcc tctgttagcc atcaaatgat
    10261 ctgggatggg ctagaagtca tgccggtcgt ttccgatgac ttgacattgg aaggcttgat
    10321 tacccggcaa gatgtaatga aagcgatgca gcttgtccag cgacaaccgc agattgcaga
    10381 tacgatttcg gatcagatat ctggaaatat ccatacgatt gatactgacc gtgaaggaaa
    10441 tcgtttggaa aaccctaagt tcaaattcgt agtagcacca caaatggtca atggcgttgg
    10501 aaccatttct tttggcgtac ttagcgaaat catttctgat gtcgcacaaa aaacaatggt
    10561 gatgaaccaa aaacgaaata tcttgattga acaagtgaac ctacattatt tgcggctgat
    10621 ccaattggaa agcgaattag acattcgtcc acgtgtattg gaaatcggca gacgttccgc
    10681 taaactggat atcgaagtgt ttattgaaaa caccatcgtt tccaaagcaa tcgttgtttg
    10741 tcaagtaatg gaacgttctt agaaagcagg agtgaaatga cagtacagca agaaatagta
    10801 gatacaataa aaaaatacga taaaatcatt atccacaggc atttgcgccc tgatcctgat
    10861 gcattaggat cacaagtagg attagccgaa ctattacggg aaagtttccc agaaaaagaa
    10921 atacgtcaag taggtgaaac gattgaaggg cttcgctttt tagcagaaat gcaagaagtt
    10981 gaagatgtct tctaccaagg agcgctagtc atcgtgactg atacagctaa ttcacccaga
    11041 atcagtgatc aaagatatca gttggcagaa aaactgatca aaatcgacca tcatcccaat
    11101 gatgaccctt atggagatct tgtgtgggta aatacaaaag caagtagctg tagtgagatg
    11161 attgcagaat ttgctttgat gtttcctgaa tctttaaaga tgaacgttaa ggctgcacgg
    11221 ttactttatg caggaatcgt cggagataca gggcgctttt tgtatccctc cacgacgtcc
    11281 cgtacattag agattgcttc gatattgaga aattatccat ttgatgcaag tgctttgaac
    11341 agagagattg aacaaatgcc gatgaaagtc gctaaacttt ccggttatct ttatcaaaat
    11401 atccaggtgg atgaaaatgg tgcaggaaga ataatccttc cgcaagaaat tcttgataaa
    11461 tatggaatcg atgattcaga gacagcttcg atcgtttcac ttcctggagt gattgacgaa
    11521 gtgctggctt gggggatttt tgttcagcag ccttcagggt attaccgcgt acgtttacgt
    11581 tctaaggggc cgatcatcaa tgaattggct aaacgccatc atggaggagg ccatccatta
    11641 gcaagtggtg cgaatgcaaa agatttagca gaagtagagg aaatctataa agagatccaa
    11701 gatatatgcc aatcgtataa gagcagaaaa acagaatgat acttcgttcg aaaacgtgcc
    11761 ttttgtagta aaaatcacaa atgtatcttt tcaagatctt ctttttccgt tacaatagtg
    11821 ctcactaaag attttaaagg agaaagaaga tgacattacc aaattgccca gaatgtggct
    11881 cagagtacac atatgaagat cgaggcttgt tgatttgccc agaatgcggg aacgaatgga
    11941 acaaagaaga acaagaaact gtcatagaag acggattagt ggtattagat gcaaatggaa
    12001 accgtctgtt agagggcgat agcgtgacag tgattaaaga cttgaaagta aaaggtgcgt
    12061 caggtgccat taaacaagga acaaaagtca agaacatccg tttagtagaa ggcgatcata
    12121 atatcgattg caaggtagaa ggatttggac cgatgaaact gaaatcggaa tttgtgaaaa
    12181 agaattaaag aatattcaag gtcttttcac attcttttcc tactactaat gataaaataa
    12241 agtaaacgaa aaggagtgaa acgaaaatgt atggttatgg aatgtatggt tttttagatc
    12301 cgacatttct tttggtcatt cttggattag ttatttccgg aattgcgtca gcgtacgtca
    12361 atagtacttt ccgaaaatac gatcaagtga gaagcaaaaa caatgtgact ggtacacaag
    12421 cagcacaata tatattacaa agtcaacaga taaataatgt cggtgttcaa caaattgccg
    12481 gagatctcac agataactac aattcaggta ataagatgtt gagtttgtct caagcaactg
    12541 cacagtctac ttcagttgct gcgataggtg tagcagctca tgaatgcgga catgcggtac
    12601 aagatgcttc gggttatgtc ccattgaaga ttcgtgcagc acttgttcca gcagctaact
    12661 ttggttcgat gatttcgttc cccttgatta tggtaggtgt attgcttagc tggaactcta
    12721 cgttaatcaa tatcgggatt ttcgcttttt ctttagcatt acttttccaa ttggtgacat
    12781 taccagtcga atttaacgcg tctcggcgag caatccaaat tttgagtgaa ggcggactac
    12841 tgacggaaga agaagtaccg atggcaagac acgtattatt tgcagcagca ctgacttatg
    12901 ttgcagcagc gctttcgaca ttcctgcagc tgttgcgctt gatcttattg ttcggcggca
    12961 accgaagaga ttaaaataaa gaagttgtga aatcagcgct ttgatctgag ctaataccga
    13021 gagttagctg attgaatacc atttatttgg aataagggct ctttgtcaat aaggactgat
    13081 gggttgtaca aaatcaaatc tgggtcagaa tgaacctcat tctggctcag
//
LOCUS       DAPCMT010000081        12933 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPCMT010000081 DAPCMT010000000
VERSION     DAPCMT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.083"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            133..3660
                     /locus_tag="RD743_001854"
     CDS             133..3660
                     /locus_tag="RD743_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDU0563662.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="RD743_001855"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="RD743_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDU0563663.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="RD743_001856"
     CDS             7448..7789
                     /locus_tag="RD743_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0563664.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="RD743_001857"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="RD743_001857"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDU0563665.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="RD743_001858"
     CDS             9146..11566
                     /locus_tag="RD743_001858"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDU0563666.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="RD743_001859"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="RD743_001859"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDU0563667.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4395 a         2247 c         2740 g         3551 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cag
//
LOCUS       DAPCMT010000082        12746 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPCMT010000082 DAPCMT010000000
VERSION     DAPCMT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.084"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..575)
                     /locus_tag="RD743_001860"
     CDS             complement(<1..575)
                     /locus_tag="RD743_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDU0563668.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFF"
     gene            717..1649
                     /gene="pfkB"
                     /locus_tag="RD743_001861"
     CDS             717..1649
                     /gene="pfkB"
                     /locus_tag="RD743_001861"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDU0563669.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            1631..3094
                     /locus_tag="RD743_001862"
     CDS             1631..3094
                     /locus_tag="RD743_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0563670.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            3091..3549
                     /locus_tag="RD743_001863"
     CDS             3091..3549
                     /locus_tag="RD743_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0563671.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            3546..4520
                     /gene="lacD"
                     /locus_tag="RD743_001864"
     CDS             3546..4520
                     /gene="lacD"
                     /locus_tag="RD743_001864"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDU0563672.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            4961..5689
                     /locus_tag="RD743_001865"
     CDS             4961..5689
                     /locus_tag="RD743_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0563673.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            5707..6903
                     /locus_tag="RD743_001866"
     CDS             5707..6903
                     /locus_tag="RD743_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0563674.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            6896..7891
                     /gene="lacD"
                     /locus_tag="RD743_001867"
     CDS             6896..7891
                     /gene="lacD"
                     /locus_tag="RD743_001867"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDU0563675.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            7888..8829
                     /locus_tag="RD743_001868"
     CDS             7888..8829
                     /locus_tag="RD743_001868"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDU0563676.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            9005..9484
                     /locus_tag="RD743_001869"
     CDS             9005..9484
                     /locus_tag="RD743_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0563677.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            9497..10387
                     /locus_tag="RD743_001870"
     CDS             9497..10387
                     /locus_tag="RD743_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0563678.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            10374..11183
                     /locus_tag="RD743_001871"
     CDS             10374..11183
                     /locus_tag="RD743_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0563679.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFVALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            11197..11598
                     /locus_tag="RD743_001872"
     CDS             11197..11598
                     /locus_tag="RD743_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDU0563680.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11644..12594)
                     /gene="yidC"
                     /locus_tag="RD743_001873"
     CDS             complement(11644..12594)
                     /gene="yidC"
                     /locus_tag="RD743_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDU0563681.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         4093 a         2058 c         2783 g         3812 t
ORIGIN      
        1 ttaaaaaatt gaaaaataaa gacataaatc ttatttttga tgtttttatt tgttttctca
       61 ccgttcaaag aaacaaaaat aacgctgtct ttcttagtta gtttccgact gatgcttttg
      121 ataatttctg gatcagtatg catctgacaa ttttttcccg ttaattgaag ttttaccagc
      181 atttcttggc caatcatttc tgaaaatcct cgggcaaaga caaaaacccg gtcagcaaat
      241 ttgattcttt gtattgcatc ttcaatgttc ccgatttcca acatattgat tgtacgtacc
      301 acttcttgtt cattttttag aatggctcgg cggattcctt tgtctacttt atctaaaaat
      361 gaaaaattga tgttcttgtt ttctccttct tttagatgat gcttaaacga agtaaaccct
      421 tcatagcctt tctttttcat tgtccgcaca atggtggctg ttgagacatt tgcttgttca
      481 cttagtttca cgatggaata gttagggatc tctaggatga ggtgttcgat aaagttccag
      541 aggtactgct ctgtttcact taatttgtga ttcatattct ttccttcttt caccaaaagt
      601 tagttaaaaa aagcacgacg agtttataaa tgtaaaggtt ttctttgtgt tgggacgatt
      661 aagaaaacat tttctatttc gcgtatttca taatagaatc aaggaggtcg ttggttatgt
      721 ctatctacac atgcacgatg aatttggcaa ttgatctatt tattgaaaca gatgagatgc
      781 atccttttat ggtaaaccgc acaaaagagg atgacattca ggcaaacgga aaaggggtga
      841 atgtgtcact agtattaaag atgctagatt tcccaagtac ggcattaggt ttttctgctg
      901 ggttcacagg aaagtatata gaagactatt tacagcagaa acaaatccaa acagagttta
      961 ttgaagtgcc tggtatgacg cgtatcaatg tatttacaca agtcaatcaa acgggagaag
     1021 aatacaaatt agtgaatcaa ggacctgaaa taccgcagac agctgttcat aattttttaa
     1081 atcaaataag agcattacaa gcaggggatt atctttgtgt ttctgggagt ttaccaagag
     1141 ggctgtctcc aaaggtttta atagaaatca gtcggatttg tttgattatt gatagtagtg
     1201 atcaagaagt catggattgt ttaccctatc gaccgttttt gctgaaacca aatgaggaag
     1261 aactttcttc ttggttcgga cgaaaaatgg ttacagatga agattacttt gtttatggac
     1321 aacgactagt agagtcaggc gcagaaaatg ttttgttatc cctcggtgag aaaggagcga
     1381 ttctttttac gaaagacagg gtgattcgag gaaactcgcc aaaaggaaaa gttgtcaata
     1441 cagcatgtgc aggtgatgcc atgttaggca cttttttagc aggatacatg aatcgacgat
     1501 cgcttgatga aacgttacgt aaaagcatag cagcaggcag ctctactgct tttcgtaaag
     1561 gactgactga ttttttggat gtggaggaat taagtaaaca aatcaaaatc aaggaagaga
     1621 ggttcgaaag atgggaaaat atcaactaat tgcagcaacc ggttgtccga caggaattgc
     1681 gcacacctac atggcgcaag aggcattaga gcaagcagct aggaaaaaag gcatcacgat
     1741 caaagtggag acccatggac aaattgggat cgagaatgaa ttaacggctg aagaaatcaa
     1801 acaggcagat gctgtcatta ttgccgcaga caaagatgtc cattcagaaa gatttgtggg
     1861 aaaacgagtc attgatgttt ctgtcagtac aggaatcaag gatgcacaac ggctgattga
     1921 cgaagcattg gcaggaaaag gtactgtctt agcagataat gaagctgctg atactttaga
     1981 accagaagcg ataagtggtg ctaattttgg tcgtagcatc tataaaaatt tgatgaacgg
     2041 tgtctctcat atgttgcctt tcgtggtagc tggcggagta ttgattgcta tttcatttgc
     2101 cgtttgggga atctattctt ctgatccaga tcatgcacaa tacaatgcta cagcggcaat
     2161 ggtaaaagca gttgacgatg cagcaatggg aatgatggtt ccagttttag cagcttatat
     2221 tgcagaaggg atcgcgaaac gtccagggtt agtcgttgga tttgttggtg gaatcgtagc
     2281 catgaatggc ggtactggat ttttaggagg tattttagct gggttcttag caggttattt
     2341 tatcttattt ttgcaaaagc tactgataat tttacctaaa caactagatg gcttgaaggc
     2401 aatcgtctta tatccagtta ttggtgtagc atttattggc atcgtgatgt ctttgctggc
     2461 aggtccaatg aaggctataa atgaaggaat gatgagcttt ttagctggat ttgaatattc
     2521 gaatccactt gttcttggcg tgattgttgg ctgtatgtgt gcgtttgata tgggaggacc
     2581 ggttaataag gcggcatatg taacaggaac agctcttttg acacaaggaa acacgacttt
     2641 tatggctggt gcgtcagcag cttgcattgc accaccgttg attactggtt ttgctacttt
     2701 attgtttggt aaatattttg atactaatga tcgtaatgca ggtttggtta attttatttt
     2761 aggatcaact catattacgg aaggagctat tccgtttgct gcgaaagacc cgatccgagt
     2821 attaccaacg atgatgttag gctcatcaat tgctgctgtc ttgacctatc tgtttggtgt
     2881 ccaagtacca gccccacatg gcggtttttt agtgttacct gtagtgacgg gaaaaatcca
     2941 atgggttaca gcgattctta ttggttcgat cgttggtgga gtgatactag gtttgatcca
     3001 aaagaaaaga gtaactgata aagacaagaa gagggataat aaagcaacaa atcgtacagt
     3061 tatcgaagaa ggaagagcag gaggtttatc atgacaatca ttcatgaaga gaacattttt
     3121 ttacaacagc cattagataa tcaagaagaa gcatttaagt ttttagcaaa gcaaacattc
     3181 tgtttaggaa tcgctcatga cgaaaaagaa gtatatcaaa aacttctgga aagagaaaaa
     3241 gaaggtacta caggaataat gtcaggtttt gctatccctc atgcgaagtc tcaaacaatc
     3301 aatgaaccta gtttgattat cgtcacactg aaaaaaggaa ttgaatggaa cagtttagat
     3361 agacagtcag ttgattttat ttttgcgtta tttatcccag attcagaagc aggaacgact
     3421 catttgaaat tattgtctct cgttgcacga tcattgatgc gaaaagaggt tacagaacag
     3481 ttgagagctg caaaatcaaa aatagaaata gctacaatat taactaaaga aataggtgaa
     3541 gatatatgaa gaaaatgagt atagaaaaac aggaacgttt gaagcgattg acggatgaag
     3601 ctggaattat tagcgcgcta gctatcgacc aacgtggtgc tttgaagaaa atgatcaacc
     3661 aatataaaga agctacatca gaagatatca tccaatttaa acagatggtt tctcagtacc
     3721 tgactccgta tgcttccgct attttactcg atccagaata tggtctgcct gcagcagcgc
     3781 ttcgagagaa agaatgtggt ttgttgctag cttatgaaaa aaccggctat gatgcgaaaa
     3841 tgccaggccg actaccagat ttactgaata gttggtcagt gaaaaggtta aaagaagtgg
     3901 gtgcggatgc ttgtaaattt ttattgtact atgacgttga tgaaccagta gagattaatg
     3961 tgcaaaaaca aatagttata gaacggattg gttcagaatg cgaagcagaa ggcatacctt
     4021 tctttttaga gctcctttct tacgatgcaa ctattggcga tccgtcttct cttgactatg
     4081 caaaagtaaa accgcataaa gtgattgaag ccatgcgaga attttcaaaa gaacgttatc
     4141 aagtggatgt attgaaagta gaagtaccag tgaatatgag atttgtggaa ggatatgggc
     4201 aagatacttg ttatagtcaa gcagaggcat ccgcgtattt ctatgaacaa tcgaaagtga
     4261 ctgaacaacc gtttattttt ttaagtgcgg gagtcagcgc tgcactattt caagatacat
     4321 tgcgttttgc aaaacaatca ggttcgacat tcaatggtgt cttatgtggg cgagcaacat
     4381 gggcgaaggg agtagcacct tttgttaccg aaggcgaaga aaaagcgagt agttggatgt
     4441 tagaagaagg aaaacaaaat attaaagaat taagtacact attagaagaa acagcaactt
     4501 ctatttatgt taacaagtaa gagaaaggat tgtctgagtg acagattcaa aaaataatta
     4561 gttatttaaa aaaatgtttg tcaaatttta tagaaaaaaa gtataaaact gcaaaagtta
     4621 tctaaaacta ataataacat ttgtcattaa taagctattt ttagttgttg taaatgatat
     4681 tggaagtaat aaaacgatag ttgtaggaat atcataggga ttaatgtgga tcacgctgct
     4741 cgataagtta gaccattttg acggatcgct tatgtggttc gtcttttttt ctgttctttt
     4801 attggtaact ggtatttttg aggtatcttt gtctattcaa gatataaagc tttaaagagc
     4861 tgttataata gcttttttgg tctaaaaaac ctttgacaaa tgattaataa ttcactattc
     4921 ttgtgttgtg gttataacca gtaaagaaga ggtgattttt atggagagct caaataaagg
     4981 aaaatcattg tattatcaac tagttgattt attgaaggat cgtatcgaaa cacagatgac
     5041 tccgcatgat aagctgttat ctgaaagaga actttgtcaa cactatgggg tgagtcgtac
     5101 caccgtacgt ctggctttac aggaattgga aaatcaaggt tatatttacc gaagacatgg
     5161 aaaaggtacc tatgtctctg atttgtctga taagtcatcg aacttagctg gagcatatag
     5221 ctttacagaa gaaatgcgcg aaatagggcg cgtccctaag acggtcattt tagaatttag
     5281 acaaatggaa gctaataaga gaattgctaa gaatttgaac atccatttag gtgaaactgt
     5341 atttaaactt aaaagattgc gtttggcaga tgaagagcca ttgatggttg agcgttctta
     5401 tctaccggcg aaactattta tgggacttga tttgccgtta cttgaaagta aaccgttgta
     5461 tgatgtgttt tcacaggatt atgaacaaca agttcaatta gctgatgaag agttttatgc
     5521 aagtatcgtg caaaaaaaag atgctgttgt gctacgaatc gctgatggcg ctcccgtctt
     5581 acatctgacg aggataacct ataacgtgaa aaatgagatt atcgaatata cagatagtgt
     5641 agcaagagcg gaccagtttc attataaaat tcggcatatc cgaaaatgat tgagagggag
     5701 aacaacatgt ttgagctatc gaagaacaaa ttaaaacaaa taggtgcagc aatcacgaca
     5761 caagaaataa aacaacaacc agatctctgg aaagaaacaa tcgatagatt gatgcaaagc
     5821 caagaaaatt tagctctatt ttttgatcaa ataaaaaaag aagcaaacgg aagaagaata
     5881 cgtgttattt ttacgggtgc aggaacgtct caatatattg gggatacgct cttaccctat
     5941 ctaaatgaac atggagacaa tcagtttttt atcttccaca gttttggcac gacagatatt
     6001 gtagcttcac caaaagaatt tttgtttcca aatgaaccga ctttattagt ttcatttgct
     6061 cgcagtggca acagtccaga aagtgtagcg gctgtgcaaa ttgcaaatga acaagttgaa
     6121 acattgtttc atttgacgat tacttgtgct aaagagggga tgttggcaaa aaacgcacag
     6181 gatgataaac agaattatct gttattgatg ccagagagag caaacgatgc aggttttgct
     6241 atgacaggaa gttttacgtg tatgttattg agtgctttat ggatctttga tcaaacagca
     6301 atggagaaaa aagaaagata tcttgaacaa ttagttaaaa tcggtcaaga tcttctttca
     6361 agagatcaag aaattcaaaa gttattacct gatttttttg aacggatcgc ttatctgggt
     6421 tcaggaagtt tggcaggtgc gacaagagaa gcacaattaa aaattttaga attaacagca
     6481 ggtaaagttg ctacaatttt tgattcttcg atggggtttc gtcatggacc aaagtctttt
     6541 ttaaatagcg agacactcgt tgtaggtttt gtgagcaatc aaccatatac gcgaaattat
     6601 gacttagata ttttagagga aataaaagaa gatcgggtca ctgccaatgt cattgcgatt
     6661 gcgcaaccgg gtactattga tttctcaggt atgactgtgc tattggaaga ctcggtgagt
     6721 aaattgcccg atgcctatct tgcattagcg atgattatga ctgcacaaat cattgcactc
     6781 tatacttcaa tcaaagtagc taataaacca gatacaccat ctccaacagg cacggtcaat
     6841 cgagtagtca agggcgtcat tattcataag tatgaaaccg aaggagaaat gacagatgaa
     6901 tgaagaaaaa aatagggcaa tggagagact atcaacaaaa gatgggatta ttagtgcatt
     6961 agcgatcgat cagcgtggtg ctctaaaaaa aatgatcaaa tcgttgggaa aagagccaag
     7021 tgaagaccaa atcagtgaat tcaaagaact agtatcagct gagctgacac cctatgcttc
     7081 tgctatttta ttggatccgg aatatggatt gccagcagca aaagtcagag caaaagagac
     7141 ggggttattg ttagcttatg agaaaaccgg ctatgacgca actacgcctg gtcgtctacc
     7201 tgatttatta gcggattggt cttctctcag attgaaagaa cagcaagcgg atgcagtaaa
     7261 attcttactt tactacgatg ttgatgaagc acccgaaatc aatcatttga agcatgtgtt
     7321 catcgaacgt ttaggtagtg agtgcgaagg agaaaatcgt cccttctatt tggagttagt
     7381 ttcttatgat gcacaaataa gcgatagttc ttctccagag tatgcgaaag tcaagcccca
     7441 caaagttatt gagatgatga aagaattttc tgatccaaaa tataaagttg atgtgttgaa
     7501 agtagaagta ccagtcaata tgaattatgt gaatggctat tccacaggtg aatttattca
     7561 cagcaaagaa gaggctgctg ccttctttaa ggagcaaagt gaagcgactc cattaccatt
     7621 catttttttg agtgggggtg tttcaacaga attatttcaa caaacactga tttttgctca
     7681 tgaatcgggt tccaccttca atggtgtatt gtgtggacgt gcgacatgga aagatggggt
     7741 caaagcatac gtcgaaaatg gaaaagacgg agctattcat tggttaagaa caaatgggag
     7801 agaaaacatc gagactttaa ataaagtaat aaatgaaacc gctacccctt ggttcaataa
     7861 aacaaaaagc aaaaaggtga agatgaaatg attacgacag ttaccttgaa tccttccatc
     7921 gatatcgcgt atacaattga agaattaaac ctcgacaaag tcaatcgagt tgaaacagtc
     7981 aataaaacag cgggaggaaa aggagtgaat gtggcgaggg ttctaaattt attaggagaa
     8041 gaggtgatag ctactggttt aataggtggc cagcacggtg aatatctaaa gaaccagcta
     8101 gatcagttag ggatcatgca tcagtttgtt ccaattaaag gagagacaag ggattctatt
     8161 gctatcctgc atagttcgag acaaacagag cttttggaac gaggcccctt cataacaaag
     8221 aaagaacaac agacatttac cgatcatttc gagcagctcc tcgaaatatc agatattatg
     8281 acgatctcag gtagtttacc ccaaggatgc cctcttgatt actataagca attggttgct
     8341 ttagctgata aaaaagggaa agacatatta ctagatactt ctggtaaatt tttgcttgaa
     8401 gtattggaag gtaaggagaa gccactactt atcaagccca atctggaaga attaggagaa
     8461 ctgattggcc aacgtatctg ttcttcgatt caattagaaa aaattcagtc cttattagct
     8521 catccaatat ttgaaggagt gccatgggtt gttgtttctt taggagagag aggtgctgtg
     8581 gcaaaagttg ctcagtgttt ttacgaagtc attattccta aaatatcagt tgttaatcct
     8641 gttggttcag gtgacgcgac ccttgcagga tcggcccatg cgttatcaaa aaggcaatca
     8701 atcgaagaga ttctcgcatt ttctatggct tgtggtatgg caaacgcgca acaagagaaa
     8761 actggctgga tcgaccctat gaaagttacg atgttgatga aacacattca agttaagaaa
     8821 atcaaatgaa cttaaatata aaagaaagcg ttttctttag ttgggaagga atttgttctt
     8881 agcttaaaaa tgaaatataa gaaagaaaaa atgaaaacgt tatctttgaa acagaaggga
     8941 tatttgatga tgtcacagtt ttaattgaac aataaataga aaaaatagat aaggagaatg
     9001 catgatggca attattggtg taagaatcga tggacgtttg atacatggac aagtagcaaa
     9061 tctttggaca acgaaactga atattagtcg aatcatggtc gtggatgatg aggtagccac
     9121 aaacacaatt gaaaaaagtg gattgaagtt agcgactcca gcaggggtaa aattaagcat
     9181 tttgccaatc gaaaaagcag caacaaatat tttagctggc aaatatgact ctcaacgagt
     9241 attgatcgta gcaaaaaagc cagatcgaat cttatcttta gtagaaaaag gagttcctat
     9301 caaagaagta aatgtgggga atatgtcaca aacgaatgaa acacggtcca ttacaaaatc
     9361 aatcaatgtg gtagatgcag atatcgaagt atttaaaaaa ttagatcagc ttggtataca
     9421 tttaattgct caaatggtac caaacgataa agcagaagac tttatgaatc ttttaatcaa
     9481 ataggaggaa tgatccatgg atattcaatg gtggcagatc ttattattaa cattatatgc
     9541 aggttatcaa attttagatg aattacagat ctattcttca atgagtgcgc cagtgtttgc
     9601 agggttatat gcaggtttag taatgggtga tttaaacgca ggtttgatta ttggtggcag
     9661 tatgcagttg acagttttag gagtggggac gtttggaggc gcatcaaaaa tagatgccaa
     9721 ctctgggacg attttagcaa ctgccttctc gatttctctt ggaatgaatc cagaacaagc
     9781 gattgctgct attgcagtgc ctgtagcaag cttaatgatc caattggata ttttagctag
     9841 attcgctaac acatactttg cccatcgtat tgaccgtcac gtagaagaca tgaattatca
     9901 agcaatcgaa cgcaatttct taatgggagc tttaccttgg tcgctctctc gtatgattcc
     9961 tgtattttta gctttagcat ttggtggtgg attagttgaa aatgttgttt ctgttttaaa
    10021 cgggaatcta aaatggctag gcgatggact ttcagtagca ggagctgttt taccagctgt
    10081 tgggtttgcc attcttctgc gctatttacc agtaaaaaaa catctatctt atcttatttt
    10141 aggatttacg atcacggcat tattagcaac gttattctct aatgttcaat tattaggaac
    10201 aagtgtggca agcgttgtta aagatcttcc ggcaactttc aattcgctgc cgatgcttgc
    10261 tgtagcattg attggtctag cactagcaat gatcagttat aagaatgatc aaaacaaaga
    10321 ccatcaacca ttagctagtg agccatcaca ttctgttgag ggggaaatcg aagatgacga
    10381 aatctaatta caaattaaca aaagaagatt ttaagcaaat caatcgaaga agtttgttca
    10441 cttttcaatt aggttggaac tatgagagaa tgcagggttc tggctattta tatactatct
    10501 tgccacaact aagaaaaata tatggagaca atacaccaga attaaaagag atgatgaaaa
    10561 cacatacgca attttttaat acttctaatt tttttaatac aatcattact gggattgatc
    10621 ttgcaatgga agaaaaagag ggaattgctt caaaggaaac cgtatcgggg ctgaaagctg
    10681 gattgatggg ttcctttgca gcgattggag attccgtatt tgttgctttg attccaacga
    10741 tttttggggc tttggcggcg aatatggcaa ctgatggaaa tccgataggt gtgtttatat
    10801 ggatttttgc tcagatcgtt gtcatggttt ttcgttggaa gcaacttgaa tttgcataca
    10861 aagaaggaat ttccttagtt actactatgc aatctcgctt aactgcatta acggacgcag
    10921 ccactttatt aggggtattc atggtagggg cattagttgc gacaatgatc aatgttaaat
    10981 tttcatgggc accaacgatt ggcgatgtga cattgaatat gcaaaactat atcgatatga
    11041 tcttacctaa aattttacct gctgggatcg tcggtggtgt ttattggtta ttaggaaaga
    11101 aaaacatgac tcctactcga gcgattttta tcgtattaat tgtgtgtgtt gctttatctg
    11161 cattgggtgt gatttcgaaa taagggagag cgaaaaatga gtagacaatt ggtattgatt
    11221 agtcacggaa aattttgtga ggaattgaaa aaaagtacag aaatgatcat ggggcctcaa
    11281 gaggatatct acacgttacc tttattacca gaagatggca ctgaaacata caaacaaaag
    11341 tttctcactg tcacagattc gttaaatgat tttgtggtac tatgcgatct tttaggtggt
    11401 acacctgcaa atatcatttc taaactaata atggaaggac aacagattac tctttatgct
    11461 gggatgaatc ttccgatggt cattgaattt atcaactcac aaatgattgg ttcagagccc
    11521 gagtttgttc gtgcaggatg tgaaagcatt gtctctgtca acgaattaat taatggaata
    11581 gcagaagagg aagagtaaca aaaaagatga gggcttagct ctcatctttt ttgtcctatg
    11641 tttttatttc cgcttctgct gctttcctgc gttgcggcct ttgttgtttg tactgttttt
    11701 tggttgtttg atttgttttg ttgcttgttt gttgttagct ggctttgctg attctgtcac
    11761 gtcttttctt ggagtgacga cttgttttgg cgggttttgt ttcaactctt ctgccacttg
    11821 tgctttcaaa cgtggtttca tcataacgtt tgtgatgaat gtttgcagac agctgaacac
    11881 accaccgact acccagtaaa gtgtaacacc ggctggcgca ctgatcgaca tgaagacgat
    11941 catagcagga gagacgatta gcatcgtacg catcgtcttt ttctgttctt caggaatacc
    12001 gatcgttgag atgtatcctt gaagcagata ggcgacccct gcaatgacaa caagtaccat
    12061 acttggtgag cctaaatcga tgccgtagaa agatgattcc cgaatacctt ctgtataacg
    12121 tgccgtaaag tacaaggcag agaagatcgg catttggatc aatagaggca gacagccgat
    12181 tccgccggtc atactcaatc cgttttcgcg gtatacttgc tgcatttctg cttgtgctgc
    12241 catttgttct tcacgtgttg ttgcagcttt caatttttgt tgggctgctt caacttgtgg
    12301 ttttaacgct tgcatttttt cagattggat cattgttttt ttcgattgac taatacctag
    12361 tggcaagatg atgatacgga cgatcacagt cattgcaata actgcccagc cataagacca
    12421 attgaaattc tcaactagat atgtaatcgc ttggcccata ggatggacga gtactcgata
    12481 aacgattccc gaagtgtcag ggtttccgtt tgaatccatc cgcacacagc cagataaaaa
    12541 gagcaggata ctgacaagtc cggatcccaa taaccagttt ttccatttat tcatagaata
    12601 cttcctcttc tataattttt acacaaactt aactatacta aaaaagcgca tggaattcaa
    12661 tcaacttcat agaaatttca tataaatatt cgttctttta cgtagcgaaa aattttgaaa
    12721 aaaagacaaa aaatcaagaa agaaat
//
LOCUS       DAPCMT010000083        12734 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPCMT010000083 DAPCMT010000000
VERSION     DAPCMT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.085"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="RD743_001874"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="RD743_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDU0563682.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="RD743_001875"
     CDS             complement(322..891)
                     /locus_tag="RD743_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDU0563683.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="RD743_001876"
     CDS             1077..1979
                     /locus_tag="RD743_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0563684.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="RD743_001877"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="RD743_001877"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDU0563685.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="RD743_001878"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="RD743_001878"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDU0563686.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="RD743_001879"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="RD743_001879"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDU0563687.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="RD743_001880"
     CDS             8547..8732
                     /locus_tag="RD743_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDU0563688.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="RD743_001881"
     CDS             complement(8794..9990)
                     /locus_tag="RD743_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDU0563689.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="RD743_001882"
     CDS             complement(10160..11596)
                     /locus_tag="RD743_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDU0563690.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="RD743_001883"
     CDS             11889..12698
                     /locus_tag="RD743_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0563691.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2675 c         2249 g         4007 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaacgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAPCMT010000084        12422 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPCMT010000084 DAPCMT010000000
VERSION     DAPCMT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.086"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            410..967
                     /locus_tag="RD743_001884"
                     /pseudo
     CDS             410..967
                     /locus_tag="RD743_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1025..3064
                     /locus_tag="RD743_001885"
     CDS             1025..3064
                     /locus_tag="RD743_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDU0563692.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3173..4324)
                     /locus_tag="RD743_001886"
     CDS             complement(3173..4324)
                     /locus_tag="RD743_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDU0563693.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4478..5008)
                     /locus_tag="RD743_001887"
     CDS             complement(4478..5008)
                     /locus_tag="RD743_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDU0563694.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5107..5457)
                     /locus_tag="RD743_001888"
     CDS             complement(5107..5457)
                     /locus_tag="RD743_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDU0563695.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5614..7143)
                     /gene="ahpF"
                     /locus_tag="RD743_001889"
     CDS             complement(5614..7143)
                     /gene="ahpF"
                     /locus_tag="RD743_001889"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDU0563696.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7153..7716)
                     /gene="ahpC"
                     /locus_tag="RD743_001890"
     CDS             complement(7153..7716)
                     /gene="ahpC"
                     /locus_tag="RD743_001890"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDU0563697.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7860..8522)
                     /locus_tag="RD743_001891"
     CDS             complement(7860..8522)
                     /locus_tag="RD743_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0563698.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8706..10352
                     /locus_tag="RD743_001892"
     CDS             8706..10352
                     /locus_tag="RD743_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDU0563699.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10343..11415
                     /gene="uxuA"
                     /locus_tag="RD743_001893"
                     /pseudo
     CDS             10343..11415
                     /gene="uxuA"
                     /locus_tag="RD743_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
     gene            complement(11523..>12422)
                     /locus_tag="RD743_001894"
     CDS             complement(11523..>12422)
                     /locus_tag="RD743_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HDU0563700.1"
                     /translation="YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDLSVDILRPNL
                     PICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTVFDL
                     FIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMD
                     FREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKI
                     EQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRIS
                     EYIFELKEEALMDQSKKENQVTS"
BASE COUNT         4150 a         2291 c         2328 g         3653 t
ORIGIN      
        1 aaatattaaa gattgttaaa tttcactatt tcatttcaaa ttccattgcc taatcattag
       61 gaaaagtcta tcatgaactc gtaaatagaa tatagtaata accttgttag gtgaggctcc
      121 tatacaaaca taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac
      181 tgttggatta aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa
      241 aactcggacg atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt
      301 tctttatatt tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt
      361 ggattgctgt gtgaggagga gatgcggtga aaaaagatcg attggtggag gtcgctcgga
      421 ttcacttctg gttgcacaat acaaccagaa aacacaacaa gcaaaactga tctctatcat
      481 gagagattca tatgtcgata ttccaggtta cggaatgaat aagataaatg cagcttactc
      541 ttatggggga gttgatttat taaatcaaac attaaaggaa aatttcaaat ttgaagctcc
      601 ctattatgca agtatcacat ttcaagattt tatcgattgc gttaatgaac tgtttcctgg
      661 tggagtcaag atcgatgcag aaaaatctct agatttagat ggcgtatata taaacaaagg
      721 agagcaagtg atggatggta atactttact ccagtatgct cgatttcggg aagacaaaga
      781 aggagacttc gggaggatca gaaggcagca gcaagtgatc aaagctatct ctcagcagct
      841 gaaagacgta acttcaatat tcaagctacc caaagcggtt gggaaattac ttggtagtat
      901 ccaaacgaac cttccagaaa gtgtgcttct ggattgtggt atggatttcc tcaaagacat
      961 atgttagaat aaacaggtat aaatagtgaa ataaaggaat gacaagcaag agaaggagga
     1021 aaaaatgaaa agaagtgaca agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc
     1081 agtgatctta atagcagctg cgggtggcgg ttatttttac taccagcact atcaagaaac
     1141 ccaagcagta gaagctggag aaaagacagt tgagcaattt gtccaagctt tgaacaaggg
     1201 agattataac aaagctgcag aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc
     1261 tgaaaaagag atcttagata aataccagaa tatatacggt gctgctgatg tcaaaggact
     1321 tcagatatcg aatctaaaag tagataaaaa agacgattct acttacagtt tttcatataa
     1381 agcaaagatg aatacctcat taggtgaatt gaaagatctt tcttataaag gaacattaga
     1441 cagaaatgat ggtcaaacca cgatcaactg gcaacccaac ttggtttttc cagaaatgga
     1501 aggaaatgac aaagtaagcc tgaccacaca agaagcagca agagggaata ttatagatcg
     1561 aaatggtgaa ccattagcaa caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa
     1621 acttggagat ggaggcgaaa aaacagccaa tatcaaagcg attgcttcct ccttcgactt
     1681 aacagaagat gctatcaatc aggcgatttc acaaagctgg gtacaacccg attactttgt
     1741 cccattgaaa atcattgatg gagcaacgcc agaacttcca gctggagcta ccattcaaga
     1801 agtagacggc agatattatc ctttgggtga agcagctgct cagctgattg gttacgtggg
     1861 agatatcaca gcagaagata ttgataagaa tccagaatta agcagtaatg gaaaaatcgg
     1921 tcgctcaggt ttggagatgg cttttgataa ggatcttcgt gggacaacag gtgggaaatt
     1981 aagcattaca gatgcagacg gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa
     2041 tggcaaagat atcaaattga caatcgatgc gaaggcacaa aaaatagctt ttgacagtct
     2101 aggaggaaaa gctgcatcaa ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct
     2161 tgctagctct ccaagctatg atccaaacaa aatgacaaac gggatctcac aagaagatta
     2221 caaagcttat gaagaaaatc ctgaacatcc attcatcagc cgatttgcga caggttatgc
     2281 tcctggatct acgtttaaaa tgattacagc agcaatcggt ctcgacaacg gcactatcga
     2341 tccaaatgaa gtgttgacga tcaacgggct taaatggcaa aaagacagtt cttggggatc
     2401 ttttaaaaca acgcgtgtca gtagtgatgt atcacaagta gacttaaaaa ctgctttgat
     2461 ctattccgat aatatatatg cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg
     2521 tacaggctta gataaattca tttttggtga agaccttgat ttgccaatta gtatgaatcc
     2581 agcacaaatt tctaatgaag atagctttaa ctcagatatc ttgctagctg atactggata
     2641 tggacagggc gaacttctaa ttactcctat ccagcaagca gcaatgtatt ctgtttttac
     2701 caacaatggc acacttgtct atcctaaatt gattgcagat aaagagacaa aagataagaa
     2761 gaatgtcatc agcgaaacag cagtacaaac gatcgtgcca gatctgagag aagttgtgca
     2821 agatgtaaat ggtacagcac attctctttc tgctttaggg attccgttgg cagcgaaaac
     2881 tggtacagcg gaaatcaaag aaaaacagga tgtaaagggg caagagaaca gtttcttgtt
     2941 tgctttcaat cttgataacc aaggttatat gatggtcagc atgttggaaa ataaagaaga
     3001 tgatgattca gcaaccaaac gagcatcgga actattgcaa tacctcaacc aaaattatca
     3061 ataaaaaatt tctttttaag tacagtaaaa ggactgtggc gttatcgtca cagtcctttt
     3121 ttcaaataaa cggttttcac gatgtctctt tcacaacttc tttttgattt actcacactt
     3181 cataaacttt ttttgtaaaa tatttgagaa caagtggaga aataagtgta cttaataaaa
     3241 tcacgataac taaaggtgaa tagtactgtg gttcgatcaa ttgactttgc tgtccaattt
     3301 gtaaaatgat caaagccatt tctccacgcg aaatcatacc agcaccgacc atcaatgaac
     3361 tatgttgaga aaaaccggca atctgagcac caaaataacc tccaagcaat ttggtcaaaa
     3421 tcgctacaag cgtcaatata gaaataaaga gtagttgttc actaaagtca gaaaaatcaa
     3481 cttctaatcc tacactgaca aaaaatactg gtataaatac tgcatatcct aaagcctcta
     3541 cattgtggta tacttcttct ttaacctttg tctgactgac agctatccca gcaaaaaaag
     3601 caccaatgac agagctaagc ccaacaagat ccgctacgta agacattcct aaacaaataa
     3661 ctagcgatac gatgataata gaagaatttg cgtacacttt ttctgcaaat gaaagtagga
     3721 aaggcgcaat ccatttgact aataagaaaa taaaaacaaa ataaattagc tgttcaatca
     3781 aaacaagcgg taaagaagca ttgctagaag cttcccccgt tagaaaagat aggctgaaac
     3841 ttagaactaa aacgactaaa atgtcatcta ccacagaagc acccaaaatt gttgatcctt
     3901 cttttgtatt cactacgttt agttctttta atacttctac tgaaatactg acagaagttg
     3961 ccgcaagaat aatcccaaag aaaatcgctt catttcccga aacttggaat gctttgcctg
     4021 tcaaccagcc aaaaacaacc gggaagatga tcccaagcaa agcaacgaac ataccaggtc
     4081 ggaaatattt tttcaaaaga gataaatcac tttccagacc agctaaaaac attaataaga
     4141 tcacaccaat ctccgaaaag tcatggacta gaatatcagg tttgacccaa ttcaatccag
     4201 cttggcctag caaaacacca acaagtaatt gtccaatcac tgctgggata cctaatctcc
     4261 gcgagatatg tgagccaatc gttgttgaaa tcaatatcag gcataaaata ccaataaatt
     4321 ccattctcat cattcctttg tataaaatat agcccgaact tagcataaca tacattattg
     4381 aaaaaatagg aaaatatact cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt
     4441 tggtttttat acggtctatt ttaataacta aaaatgatta acgtatagtc atttttctta
     4501 ttctgatgaa attgataatc tcctggatgc ccatgaaaat caaaataaag ccgaatattt
     4561 ggaatagaag catcaaactt ttgaatggat tgaacaataa gacgaatccg ccaatcaaca
     4621 acagaatact actataaaca ctccaacttt tagcttttgt attcatgctg aagaacaact
     4681 ggaacaagct gttgatgata acagctaatc caagaagaat cggcaggata gaaacgattg
     4741 caggtgcaaa agcatagacg atcagagcta agatcaggaa aaaaagacca ctgaacagac
     4801 cgatactcca agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc
     4861 caagcaaaac aagataggcg gtgatcagat aaccgataaa atgaaaaaca gcagaagggt
     4921 ttagcacaat cgcaatccca gaaataatat aaacagcagc tcttaataag gcgtattttt
     4981 gaatattttg tttaatcgtc tcaaacatat aagaaaacct cctttgactt attttaacgt
     5041 gaactagtca ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag aatatctctg
     5101 gaaaaatcaa cttctgagga tcttttccat ttttttgcct tttgctagct catcgatcat
     5161 tttatctaaa taacgaattt ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc
     5221 acaaacaaca cctgtaatca aggaacgtga aggattgggg acaggggctt ccgcaaaaaa
     5281 ttgttcgaaa gtcacttgtt tttgtagttg atattctagt tctgtttggg aataacccgt
     5341 cagccaacaa atgatttcat ctacttcctc ttttatacga tcttttctct ccgcttttgc
     5401 tacatacata ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat
     5461 cagtcacctt tctttttttc aaattattta ctacttatag tatataagaa aaagagaaga
     5521 aaaagcgatt attcaaaaaa acaaaaagga ttgtgacaaa attcttgtca ccatcctttt
     5581 ttccgctctc tgacttttta caatcttcat agctcaagtg cgcatcagat aatcgaatgc
     5641 tcctaaagct gcagtagctc ctgaacccat tgaaacaatg atttgtttgt atggactatc
     5701 tgtacaatca ccagctgcga aaacgccggg gatattcgtt gcgccatttt tctcggtcac
     5761 tatttcattt cttggattca tttcgatcgt tctatctagc caatccgtat tagggagtaa
     5821 gccgactaga atgaatactc ctgaaacagc tagtgtatgc atctccatcg tacggcgatc
     5881 gatataggag agagattcta cctgattcgt accgctgatt tctttcgtcg ctgcatgttt
     5941 gataacagtg acattcgcaa gagagactag tttatcctgt aagatttgat ctgcttttaa
     6001 ttctggtaag aattctagaa catatacatg ttttgctaac ccagccaaat cgattgctgc
     6061 ttcaacacca gaattaccac caccgataac agcaatctct tttcctttaa ataaagggcc
     6121 gtcacagtgt gggcaataag caattccttt atttttaaac tctttttcgc ctggtacatt
     6181 gatagaacgc cagtgcgcac ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc
     6241 gttagctaag ccaatattaa taaaatgttg ctctttattt aaggaaacgg cctgttgatt
     6301 catcataatg tcgatagggt aactacgcac atgactttct acttgctgca tcaattttgg
     6361 accttctgtg taagaggtac caatcatatt ttcaatccct agtgtttcaa cgacttgtcc
     6421 gccgaaagaa tcagcgacaa gtcctgtacg gattccttta cgtgctgcat agatagctgc
     6481 actactagct gctggtccac cgccaatcac taatacatca taaggttctt tttcaaacaa
     6541 ctcttcttca atcaaagggc cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc
     6601 gctactaaat tcttctccat ttaaaaacac agttggcact gccatgattt ttttatcatc
     6661 gatttctttt tggaacatcc caccttcaat catcgtatga gtaatatttg gatttaatac
     6721 agctaatata ttcaatgcct gtacaacgtc ggggcaatta tgacaagtca agctaacata
     6781 actgtcaaaa tgcaatgttt gtgttatttt tttgatttta agactcaaag attcactgat
     6841 tttcggtgcc cgtccactaa tttgtaacat agccagtaaa aaagaggaaa attcatgacc
     6901 gagggggata ccagcaaaaa cgattccact ttctttgcca actggattga tagaaaaact
     6961 aggtgtgcgc atcgaatctc ttttttccca agagatttta ggagacattt ctgctatttc
     7021 cttcgtaaat tcagctagtt tttgactatt ttcactatta tccaaactaa cagaaaaaac
     7081 aataggagat tcaatcagtg ctaaatattg ttcgagattt tgtttagttt ctttgtctaa
     7141 catatccacc acctaaatct ttccaacaag atcaaaactt ggtttcaatg tttcgccact
     7201 ttctttccat tttgccggac aaacttcacc tgggtgggta cgtacatatt gagcagcgcg
     7261 aatcttatca atcagtgcac tagcgtcacg accgattcca tctgcattga tttccatagc
     7321 ttgaacgatt ccgtctggat caataataaa tgttcctcgc tgagctaatc cttgctcttc
     7381 atccaaaaca tcaaaaatac gagaaatctg atgagacgga tcaccgatca tcacgtattc
     7441 gattttaccg atagcatctg atgtatcatg ccatgctttg tgagtaaagt gggtatcggt
     7501 agagcaagaa aagacttcaa cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc
     7561 ttctaattca gtaggacaaa caaaagtaaa atcagctgga tagaagcaaa gaatactcca
     7621 gttccccata agatcttttt cactgacttc taaaaattct ccttgatgat aagcatgtgc
     7681 attaaattct tcgatttttt tcccaatcaa tgacatagta aaacctccaa taataattat
     7741 ttatttataa ttattgtaat ttagttggag ttgcaaagtc aatggatatt ttgttattta
     7801 ataaagatta ctcaaaaacg aaggttaatt ataaaaatag aagaggtcgc aagaaaatgc
     7861 tactgctgga aatactcggg ataacgttga gtaacagttt cctgttcttt cagcatcatg
     7921 tgtaagtgca atcccgtttg aaatcgagct tcttctggtt ttttatcaac taaagccttg
     7981 agtaaatcat aatgttgttc catgatcgtt tcccatggaa gcccctgaat ggtcactcgt
     8041 aaccagcgga aacgttctaa atgggtatta tggtcatcta accagttcca aacttctttt
     8101 cttcctgcaa tttcgaaaca agcttcatga aataaattat ctgcacggaa aaaagcgcgt
     8161 tcgtcattta ttttgactgc tttttcttgt tcttctaaaa tcgtttctag cactattcgt
     8221 gtttgtgtgt ccaatttgga acaacattca atcatgattt gctcttccaa ctgttctcgg
     8281 acaaagcgag cattttctgc agaaacaaga ttaattttgg aaacatacgt accactttga
     8341 ggtatcgtat acacgagttc ttgctgacgt agttgaatca atgcttcgcg aatgggagtc
     8401 cggccgatag caagatgttc ctccagtgcc ttttcagata ttttccttcc aggttctaag
     8461 tcagcataga taatttgttt acgaatcgct tgataagctt gtgattgcaa gctgcttttc
     8521 atctttattc ctccaaaatc atttctatga acgtagtata acagaagagg tctgaaaaac
     8581 aatctggtat gtcacattaa ctagtaaatt tgtataaata gtaaaatttt ggttgacata
     8641 gcgattttta ttgcataaaa ttcacttgaa tacaaaaact aatatgttag tttaggaggg
     8701 gaaaaatgat tgatttaaac aactggagga actttatccc tgaattatct caaaaagaag
     8761 tagaaatacc tcattttgac cgtgcatcat taagagaaaa tggacaaaaa aatcctgtct
     8821 ggatccattt cggtggcgga aatctttatc gaggatttca tgcagaaatc gctcaacgtt
     8881 tagcggatct aggcgaattg tccactggtg tcattgtttg tgaaacattc gatgaagaaa
     8941 taattgaaag ggcttaccaa gattttgatg atttgattct tgaagtggtc atgccaagca
     9001 gtggagagtt cgtcaaacgt atattagctg cgacagctga cagtctgtat tgtcatccag
     9061 attgttctga acactttgat aagatgattc gacactttga aaatcctagt ttacaattag
     9121 ttacttttgc tattactgaa aaaggttatc gattgaaaga tacaaataat aaatatatag
     9181 gaccgattgc agtcgatata aaaaatggtc caaagaagcc atcacataca atgagtattc
     9241 ttgctgtgct actttataaa cgttttttat ctggagcttg gccgattgca cttgtctcta
     9301 cagataattt ttcacaaaac ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag
     9361 aatggcaaaa ggaaggttat gtacaaaata catttatcca atacttatcg aatgaagaaa
     9421 ttgttgcgtt tccatggacg atgattgatc gtatcacccc gaatccttcc ttgtcagtta
     9481 gcaaaatgtt ggcaaaagag ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa
     9541 ataatattgc tccatttgtc aatacagagg acatccatta tttaatcatg gaagatcatt
     9601 ttcccaatgg acgtccgaaa ttcgaaaaag ctggggtatt cttgactgat cgtgcaacgg
     9661 tagataaagc agatacgatg aaggtgacga cttgccttaa tcctttgcat acgtctttag
     9721 ctattatggg ttgtttactg gggtatagga gcatagctga ggagatgaaa gatccagatt
     9781 tatcgctgct agtttctgaa atcgggtaca atgaaggcct cccagttgtg gagtctccag
     9841 gtatcattga tccaaagatc tttctggaag aagtaattac tgtgcgcttt tctaatccaa
     9901 atattcctaa tatgcctcag cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt
     9961 atggtgagac gattaaaaaa tatatggaaa tagaagttaa agacccgcag acacttgtat
    10021 tcataccatt gactctcgct gcttggcttc gataccttat tggcattgat gatcaaggaa
    10081 aaccattcat cccgagtcca gacccactat tgacagacct tcagcataag ctttccgata
    10141 ttcaactggg tacgcaagat acatcacata ttcatgagaa aatggctcct attttgtcaa
    10201 ataaagaaat ttttggtgta gatctttacc aagcagagct tggcagaaaa atagaagaaa
    10261 tgttcatcaa gatggtagca ggaccaaatg ccgtacgaaa aacattacag gattacttac
    10321 aagaacatgg aggaaattta tcatggaaat gacttttaga tggtacggat cacaggaaga
    10381 aaagattacc ttagatgaaa tcagacagat tcctgcggtc aaagggattg taggcacatt
    10441 gatggatata ccagtaggag aggtatggcc tagggagagg atcaaagaac taaaagaaga
    10501 aattgagtct cgtgggttaa cactaaaagt aattgaaagc gtcaatgtgc atgatgccat
    10561 taaaattgga ttaccttccc gtgagcagta tatcgaaaac tataagcaga caatccgaaa
    10621 cttggcagaa tatggaatag aagtcatctg ctataatttt atgcctgtat ttgactgggt
    10681 gaaatctgac cttgactacc gcttagaaga cggctcatcc acgttagctt ttatatctga
    10741 tgatattcca gatgatccaa gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt
    10801 tggattgccg ggatgggaac ctgagcgctt agctcctatc aaatccttgc ttgaagccta
    10861 tgcatcagta gacgaagaaa aacttcgaga aaattttgcc tattttctcc aatcaatcat
    10921 cccaacatgt gaagaggtag gggtcaaaat ggctgtacat ccagatgatc cgccatactc
    10981 gatttttgga ttagctcgaa tcgtaaagaa tcgagaagat ttagattggt tgtgtcgcat
    11041 agtagacagc ccttataacg gaatcactct ttgtacaggg agtattgcag aagatccaga
    11101 aaatgatgta tatgggattt tagcagaatt ttgccaaaga gatcgaatcc catttgcaca
    11161 tgttcgaaat attaaatttt tagctaatcg agatttctat gaggcaccac ataaatccga
    11221 atacggttca ttagacatgt accgcatact aaaaacaatg tatgacaacg gatttaacgg
    11281 atacatccgt cctgatcatg gaagaatgat ctggaatgag acaggaagac caggatacgg
    11341 cttatatgat cgagcattag gttaaatgga ttatgggaag caatcgaaaa aggaaacaaa
    11401 agtaaagata attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa
    11461 attcttgata cataaatccg gaggttgtta ataagcattc ggatttatgt atcttatttg
    11521 gtctaggaag taacttgatt ttcttttttc gattgatcca tgagtgcttc ttcttttaat
    11581 tcaaagatat attctgagat cctcataatc acttttttcc agtcttctgg tgtgatgatt
    11641 ccttttaaaa tatagatttt tttgatatga gaaggcaatt gtttcaaagg gcagtcgctg
    11701 ataacgatgt tgcagttctc cataaaatca ttttgtattt tgatcgattc atttgaaaaa
    11761 atttgttcta tcttaacgat cagatcttta ctgatataaa aatcacgcgt atagttcaca
    11821 ccaatcacaa ttcgcggctg ttttttgtag cgatcataca aatgtactag gtcttcaacc
    11881 atccattgca cattttcctt ttctaaaata actggtaaca agtgttgatt ttgttctctg
    11941 aaatccatat aaaattgttc aatggcggtt tggacttctt tcaatacgct atgttcttga
    12001 taaagcatga aagtaggaag gggcgtattt ggttgattga agttaagaaa tccattataa
    12061 agtttatttc gtgcgaatgc caatgagtaa agaatctttt ctttttcagg tataaacaaa
    12121 tcaaatacag tacaaaggga ctccaccatt tctcttgaat aaagaacatg aggaacgttt
    12181 gcttttttta aaagtttgta ttgctccaag cttgtttctt ccgattcgat tttcgtgata
    12241 gaaagtcgag ctaaaatatt cactaagatg cgttgttcgc gacaaattgg cagattaggt
    12301 cgcaaaatat ctacagagag atcaataaaa aagtcaatat attcatactc tgtgaattct
    12361 ttttgtacgt ctttcacaga agttgcagga aaattctctt tacatatttt taatagaaaa
    12421 ta
//
LOCUS       DAPCMT010000085        12307 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPCMT010000085 DAPCMT010000000
VERSION     DAPCMT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.087"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            287..694
                     /locus_tag="RD743_001895"
     CDS             287..694
                     /locus_tag="RD743_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563701.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="RD743_001896"
     CDS             708..1109
                     /locus_tag="RD743_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563702.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="RD743_001897"
     CDS             1111..1482
                     /locus_tag="RD743_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563703.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1817)
                     /locus_tag="RD743_001898"
     CDS             complement(1518..1817)
                     /locus_tag="RD743_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563704.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1823..2053)
                     /locus_tag="RD743_001899"
     CDS             complement(1823..2053)
                     /locus_tag="RD743_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563705.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            2670..3332
                     /locus_tag="RD743_001900"
     CDS             2670..3332
                     /locus_tag="RD743_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563706.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            3411..6636
                     /gene="fss3"
                     /locus_tag="RD743_001901"
                     /pseudo
     CDS             3411..6636
                     /gene="fss3"
                     /locus_tag="RD743_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            6750..7064
                     /locus_tag="RD743_001902"
     CDS             6750..7064
                     /locus_tag="RD743_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDU0563707.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            7077..7451
                     /locus_tag="RD743_001903"
     CDS             7077..7451
                     /locus_tag="RD743_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDU0563708.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            7461..7805
                     /locus_tag="RD743_001904"
     CDS             7461..7805
                     /locus_tag="RD743_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDU0563709.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            7876..9228
                     /locus_tag="RD743_001905"
     CDS             7876..9228
                     /locus_tag="RD743_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDU0563710.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            9288..10430
                     /locus_tag="RD743_001906"
     CDS             9288..10430
                     /locus_tag="RD743_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDU0563711.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            10455..10709
                     /locus_tag="RD743_001907"
     CDS             10455..10709
                     /locus_tag="RD743_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563712.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            10965..12149
                     /locus_tag="RD743_001908"
     CDS             10965..12149
                     /locus_tag="RD743_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDU0563713.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            12146..12283
                     /locus_tag="RD743_001909"
     CDS             12146..12283
                     /locus_tag="RD743_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDU0563714.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         4168 a         2017 c         2646 g         3476 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgtaggat cgaatttaaa ccataattgt
     1801 gtctcattaa aaagcataac cattactccg atctgttttt aaaattagtt tctacctcta
     1861 atatatatcg agtttttatt ttgccttcag agaatactgt ttcttttttt gcagttacag
     1921 gttgtttatt ttcggaaaaa gctaatatag ctaaaattga aacatccatc tggaatttat
     1981 cttttttgct gctttgctca taaaagtctg catattcatc actgatattt tttctaataa
     2041 agtcttccat cataaaaatc acctcaaaac gattatacga acttacgttc tcgttgtaaa
     2101 gcgatatttg aggggcaaaa agggggcaaa aatacgttta tctttgatat atctcagatt
     2161 gaacatagtg tttattatat gtcggtattg ttgatttata gctgttttga gggtattaga
     2221 tacataatag aatatagtgg ttttgtttac gtgcattaca cggaaaagct gctcgtatca
     2281 aagaaatcag aagataaaca cggaaaactt ccttggaaat ctgaaatttg tgaaaagtcc
     2341 ttgtatatca agggcttttt cttttttctc tatcacagtt ttaagccatt tttggctgtt
     2401 ttggaccatt tttcaatcat attcccttaa aaattgtcct agattgtccg acattgacta
     2461 tcagtgtcgt caaaataggg caaaatttag tagtcagctt gacgacaaat ataatacgaa
     2521 aaataccaaa acatttaccg aatttagcaa cttttgaacc caagtttctc aaagggtcag
     2581 ctattagcaa aaaagacaaa acatgtactt ttctactaaa aagttgatgt gataggcttc
     2641 tatctaaata actattagga gtttttatcg tgaagcaaaa tgatggacgt atcatttgga
     2701 aaaatcaaag tgagttaaaa ctgattttaa caattaatga attcatcgaa aaacacggaa
     2761 ttacatctag ccgtcaatac cagaaaaagc tatcagaaaa tccaaattct gcaccaagta
     2821 tgtggttcat taacaaaaaa tatggatcat gggagaattt attgattagt ataggacgag
     2881 aaaatactgg gtatggaaaa tgggctagaa tgtcagaaca agaattattg gaaatagtag
     2941 aagcctttat taagtgcgag aagataacct ctcaacgaat gtacgaacaa aaatcagtag
     3001 ggaagaatat tccttcactt agtaccatta aaaagatgtt aggagatata cgtccacttt
     3061 ttaaagaaaa aaatgatgga tctcgattta ctgatttcga actgctgtta gaactaaaaa
     3121 atgaaataat ccgattaaaa ctacaggatg atttatcaat gacaaagttt cgaaaactag
     3181 ttcaatcccc aaaactacca tcagttgata caattatgaa aagaacgaac aaaaattggg
     3241 aggaattgat ggcagagatt ggatttgatt atcgaagaat caaaatttat aagcagagaa
     3301 acaacctatc caagacaaag aaaactaaat agtatcactc taaatcagac gttcaaaatt
     3361 aaaatttgga cgtctttttt attacccaaa aacgaaagga gatttatttt atgaaaaagc
     3421 caagatttaa gaattggcgt ttgctcgcaa cactgagtct attatgccaa acaattggtg
     3481 gatcgcttgg cccaacgatt gcctttgctg atgaaattac tcatccacaa acggtaactg
     3541 tggaattgga tttggctcac cagtatgctg tcgaaggtac atttagcgat ggtcgtccca
     3601 tgtcagaagt taccgttccg cactatgcag tttacaatgg tgtgaagcaa gatattttct
     3661 gtattgagcc aggggtgccg atttacaatg agttcactcc tggttatgag aaaaatccat
     3721 tgcctgatat gtcggagaaa gcgaaattag tttccgtttt atggaaggaa gctggtacgg
     3781 atgtggatac acatattgtg gcccagaaga tgatttggca agaagttaat ggctacgctt
     3841 cactcaatta aacgttctga tggtagtgca gtaaatatcg cagccattga gtcaaaaatc
     3901 aatcaagcca ttgcggatta tcaaaagaaa ccaagcttcc ataactccac agtgaaaacc
     3961 gtgttaggtc aatcgactac tgtgacagat acgaacggct tgaatttatc ggagtttgat
     4021 gaagttgtcg agaatatcgc aaatattgat tatcgtgtga acggcaatca actagtgatt
     4081 actccaaatg ccaattcaaa agagagtggt gaattaaccc taaagaagtc agctaataca
     4141 ggaacaccgg tagcttataa aatggctggt caacaaactt tgatggcggg ggcaattgat
     4201 aagcccaata cctacacaat caaaattgat gtggaaactg agggttcttt gaagattaaa
     4261 aaagtcgata aagaaacagg aaatgttgta ccgggaaccg ttttccattt agactttgga
     4321 aaaacattac ctgcaaaaga cgtgaccacc gataaagaag gcattgcgac attggatgag
     4381 attcctcatg gcgcgaaagt aaccattact gaaaaatcag tgccagctcc ttatacgatt
     4441 gacactacac caatcaccgc aaccattaaa tctggtgaga ctatttctgt gacttcaaaa
     4501 aatactcgcg aaaaaggtca aatcattttg aataaaatgg gggtggaaac aggaactgat
     4561 ctttggaacg acaaatactc attagccgga aatacgtttg ccattcgcaa agacagtcca
     4621 accggtgaaa ttgtccaaga aatgaccacg gatgaaaaag gtcatgcgga aacaccaaaa
     4681 gagattgcca atgcgttgga attgggaacc tattatgtga ccgaaaccaa agctagtaat
     4741 ggctttgtga atacctttaa accagtaaaa gtcgaattga actatgtcaa tcaaaccgtg
     4801 gctcttgtta ccagtaacgt aaaagggcaa aaccaagaaa tcactggtga aaccactttg
     4861 acaaaagaag acaaagatac cggtaatgaa actcaaggta aagctgagtt taaaggagct
     4921 gaatatactc tctttactgc aaaagatggt caagcggtca aatggagtga ggcttttaaa
     4981 ccagagttag tgaagggaac gaaagcttct gatgaaacgg tgactttgac tttagatgaa
     5041 aagaatcaag ttgctgttaa acacttagcg attaatgagt attactggca agaaaccaaa
     5101 gcacctgaag gatatacctt ggatgaaaca aagtaccctg tttccatcaa aaaggtggat
     5161 gataacgaaa aaaatgccgt gattacccga gatgttacgg caaaagaaca agtcattcgc
     5221 tttggctttg atttctttaa atttgctgga tcagctgctg ggactgccga aactggcttc
     5281 aatgatctga cctttaaagt atcgccattg gaaggaacca gtgagattac aggtgccgaa
     5341 gatgaagcaa ttacagctta taacgagcaa ctaggttttg atggctatgg caagtttgaa
     5401 aatcttcctt atggggatta tttacttgaa gaagtagagg ctccagaagg atttcaaaaa
     5461 attacgccat tagaaatccg ttctactttt aaagaaaata aagcggatta cactaagagt
     5521 gaatacattt ttacgattac cgaaaaagga caaaaacaac caattaaaac ggtgaccgtt
     5581 ccttacgaga aactgacgaa taaagcattt tctgttagct tgaatcgttt gatgctctat
     5641 gatttacctg aagaagaaga tagcttaact tctcttggga cttggaaaga cggaggcaaa
     5701 gaattgacag accttgattc taccgagcta gttgataaat tgagctataa cttgcatgaa
     5761 atcaaagaag actggtatgt ggtagcgcaa gccattgatg tggaagcgac aaaagctgcc
     5821 caagaaaaag atgaaaaagc caaaccagtg gtaattgccg aaacaaccgc aacgttggcg
     5881 aataaagaga aaacaggaac ttggaaaatt ttgcataaat taaccgcaga acaagttttg
     5941 gataaaacca tcgtcttatt caactatgtg tatgaaaaca agaaacccta tgaagcaggt
     6001 gatgatccag tagcgaaaga tgctagcttg aacaatcaag cccaaactgt caattgtacg
     6061 gtggaacgcc atgtttccat ccaaacaaaa gcccacctag aagatggttc gcaaaccttt
     6121 actcatgggg atgtggtgga tatgttcgat gatgtgtcga ttacccatga tgtactggat
     6181 ggctcaaaag aagcttttga aacaattctg tatgcattac ttccggataa tacgaccaaa
     6241 gaaatttgga aatctggcaa aattgattat gaagtgaatg acaaagaatt cactaaaacc
     6301 gtacttgcgg aaaaagtgga cacaagtaaa tatccagaag gtacaagttt taccttcaaa
     6361 gaaattaact atggcaaaga cggaaacatc aatgggaaac acaatgagga tctaaaagaa
     6421 aaaactcaaa ctttaacacc taaaaaagtt ccgacaacac caagtgcacc agaacaaccg
     6481 gaaataccaa ctgttccaag tgactctcaa gaatctagtc ctacagtaaa gacattccca
     6541 caaacagggg agaaaaattc taatgttctg ctgtttattg ggtttacctt gatctttgcg
     6601 actgctgggt attatttctg gaatcgccga aactaaggtg atgtgatgaa acaactaaat
     6661 aggaaaaacc aacgtgcgcg accgcctcct aaaaaaagtg gtcgcgctat ttttgtatcc
     6721 aaaactaaaa atgaaaagag gaaaagaaga tggaattgaa atttgttgtg ccagatatgg
     6781 ccgaaacctt tggaaagata aggtatgccg gcgaaggcga agttttaact gaaggatatg
     6841 ggcggaatac tacagtaatt ggtcgtagtt atcatttgta ttccagtaag caacgagccg
     6901 atgatattga agtggtggta gctgcggaag ccggcgaaaa ggattttgat caagatcaac
     6961 cactaaaagc cgtgaatccc catttggttg ccaaaggcta tgagattgag aatcgtgggt
     7021 ttaccgacta cgtgttgtat gtcgatgatt tagtgaaagc ataggaggaa aagaagatga
     7081 gattaacaga aggtattgtt gtagattcag gactaacgtt tgggaaatta cgattttccg
     7141 cattacgccg agaagtacgg aagcaaaatg aggatggaac ggttagtaac gaggtaaaag
     7201 aacggactta taatttgaaa tcctctgcgc aaggtcgaat gatccaagtt agtatcccag
     7261 caaatgtacc cttacgcgag tttgcctatg acgcagaggt agagttggtc aatccaatca
     7321 tggatacggt cgccaattat gttttccgag aaggaactac tgtcaattgg ttcattaagg
     7381 ccgatgattt agtcttgaaa cgacaaccag atcagggaaa ttctacaaac caaaatgaag
     7441 gaaagaaata ggtgaagcgt atggaaatta atgttgaaca tattttagat tgtctcgatc
     7501 aatatggaaa aggtgagcta acggaggaac aactcactaa agcattaacc tatgatgaaa
     7561 aaatgtttct gattatgcat caagggttac tggaagtagg taacgatgcc gaagaagatt
     7621 ttgatgtact gaattggtta ggggaagaag agtccttctt tatggttatc gaagtaaatg
     7681 aaagtctatg tcgagaagcc gaaaccgtat tggaagaaat tggtgtggaa atgccggatg
     7741 ccattgaagt ttttttaaag caactagtgg aaacgaaaca actccctata gcagtaaatg
     7801 actagcataa ctgcttgaaa ttggatcaat gaaatcctat gaagcttttt tcgattggag
     7861 gtggaaacca taaaaatgta caaagggcat cgtatacggg caggagatca gcacttggtt
     7921 tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag cgtcttttat
     7981 tttcaagagc ttagacaatt tgatatttct aagctatcac tttctactat agaaatcgtt
     8041 cgttctataa aagatttaat ctatttgcta ggaagtctcg ttctttcagg aagcacgatg
     8101 ttgctttata tacacttttt tcaggatcat tggcgcagtt tgtggcatcg acaaaagcta
     8161 gcccggatga ttctagaaaa tcattggtac gaagtaaaac aaactcagag tgaaggattt
     8221 ttcaaagacc tcaatagtag tcgaaccaaa gagacaatca gttacttccc caaaatctat
     8281 taccgtatga aggatggttt actttctatt cgcgttcaaa tttcactggg aaaatatcaa
     8341 gatcagcttt taaagctgga aaagaagtta gaaagtggtc tatattgtga actagtagaa
     8401 aaggaactca aagactccta tgtggagtac actttactgt atgatataat tgccaatcga
     8461 attgggatag acgaagtagt ggcagaaagt ggggctttgc gattgatgaa aaatcaagta
     8521 tgggcttatg attctttacc tcatatgtta atcgctggcg gcacaggtgg tgggaagact
     8581 tactttttac tcactatcat cgaggcttta ttaaaatctg atgccgagct ttttgttctt
     8641 gatcccaaaa atgcggactt agccgattta ggtacggtaa tgcctcatgt ctattcccaa
     8701 aaggaggaaa tttctgcttg tgtggaagat ttctatgagc gcatgatgac tcgtagcaag
     8761 gcaatgaaag aaatgcccaa ctacaaaaca ggagagaatt atgcgtttct tggacttcca
     8821 ccaaactttt taatctttga tgaatacgtg gtttatatgg aaatgttaac gacgaaggaa
     8881 agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt taggtcgtca atctggtttc
     8941 tttctgattc tagcttgtca aagaccggat gcgaaatatt taggagacgg gattcgcgat
     9001 caatttaatt tccgggtggc tttgggtcgt atgagtgaac tgggatattc catgatgttt
     9061 ggcgaggttg ataaaaaatt ctttatgaag cgtatcaaag gtcgaggcta tgtagatacg
     9121 ggagggagtg tgattagtga gttttatacg ccacttgtac caaaaggata tgatttctta
     9181 agggaaatta gcgacattgg tggattaaac gttaatacgg aaggataaaa taatagtatg
     9241 tagcctagga gtgtcaagaa tcctgggctt tttttacgag gtgatttatg acatttttag
     9301 atttattcgc tgggattggt ggcttttgct taggtatgga acaagcgggt caccagtgta
     9361 tcggcttttg tgaaattgat gattttgctc gccagagtta taaggcaatc catgatacaa
     9421 gtaaggaggt ggaaatgcat gacatcacaa gtgtatcaga cgagtttatt caatctctcg
     9481 gaccagtgga tatcctttgc ggtggatttc cgtgccaagc tttttcaatt gcgggaaagc
     9541 ggcaaggatt ttccgatact cgaggtactt tattctttga aatcgctcgg ttcgccgcta
     9601 ttctccaacc taagttttta ttccttgaga acgtccgggg attactcaat cacgaaggag
     9661 gggctacgtt cgagacgatc ctccgaacgc tggatggatt ggggtatgat gtggaatggc
     9721 aagtgcttaa ctcaaaggcc tacgttcccc aaaaccgtga gcggatcttc cttatcggac
     9781 attctcgaga cgcatgtacc gaacaagtat ttcctatcat tggatcgtct ccaacatctg
     9841 atcaaaacat cagaaactta ttaaatatca acccttccaa tcgaggaatg gggggacaag
     9901 tctatgggtc agatgacgta gcgcctacct tgaccggtga tgaaggaatc aaaatcgcat
     9961 tgccggtaga tgatggaatt tctgtagcgg gtatgttact gggaaatttt gaacaaggca
    10021 atcgggttta tgagataact ggaacagcgc ctactttgtc aacgaaacaa ggcggaacga
    10081 agatcatgct tcggaaaaac gaaacagaat atcaagaggt gaagccaggg aacagtgtga
    10141 acctggcttt tcctaattcc acaagtcgtc gaggccgtct gggaaaacaa agtgtgcata
    10201 ccttattaac aggagaccaa caagcagtcg tcacggatca gtatcaaatt cgaaagttaa
    10261 cacctaggga gtgctggcgg cttcagggct ttccggactg ggcttttgat cgagcttctc
    10321 aagtcaattc agatagtcag ttgtataagc aggctggaaa ttctgtaaca gttccggtca
    10381 tttttgatat tgctagaaga ttaaaggagg taaaaacaga tgatttatga tgagttgatt
    10441 ggggaatcta ctgggtgatt ggcaagattc agtcagatcc ggaattggaa gaagaactgt
    10501 accggctcaa ttttgatatt cgcaaaaacg gggtgaaagt ccctgatgat ccgtatgtaa
    10561 ttgatgagga aacggatgct cgaatggagc tgaatcaagt catcagtgaa ttggaacgaa
    10621 ttgcggactt tgctaaagaa cctgatatcc gccaatactt gtttgagata aaagcagagt
    10681 tggaaattga gggtattacc gcggagtaac ggccaaataa accactgata ggcactgacc
    10741 gcgggggatt tgcgaagcag aaatcctccg tggtgctgtg cctgtttatg gacggcgtta
    10801 gccggccatg atgtctcacc ccccgtatct aacagggggg tacaaaaaca caaaaaataa
    10861 gcaagcaacg ataaggaatg gctgtcctat caaggataat aagcaatttg tgtagatgtt
    10921 aactgatttg aaaaagttaa caccttaaat ttggaggtga gaacttggca caaaggaatt
    10981 tagattatcg gctattaaaa gaccggcgta atgaatatgg cgtttcacaa aataaattag
    11041 ctacgacttg tggacttagt cgcccgtact taaatcagat agaaaatggt ggcgtaaccg
    11101 catcgacaaa aaccatgagg aaaatttttg accaactgga aagtttcaat cccgagttac
    11161 ctttaacgtt actgtttgac tatgtaagga ttcgctttcc cacaacggat gcgaggaaaa
    11221 tcattcaaga gattctccat ttgaaatttg attatatgct ccacgaagat tacgcctttt
    11281 actcctatca agaacaatat gttatgggag atattgtggt gatgttgtca catgaagaag
    11341 ataaaggcgt ccttttagaa ttaaagggtc gtggttgtcg gcagtttgaa acttttttac
    11401 tcgcccaaaa gcgtagctgg tatgactttt tcgaagattg tctaaaagcc ggtggcgtga
    11461 tgaaacgctt agacctggca atcaatgatc gagttggcct gttagatatt cctgatttaa
    11521 cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg taccttcaaa agctatcgtt
    11581 ccggggaact gttgaaacct gatgataaag atggtatggg caatactttg tatattggca
    11641 gtcttaagag tgaggtctat ttctgtttgt acgaaaaaga ctatgagcaa tatatcaagt
    11701 tagggattcc tttagaccag accgaaacga aaaatcgttt tgaaatccga ctgaaaaatg
    11761 atcgagctta tcatgcaatt caagatttgt taaaaggtcg cagtattgaa agtaccacat
    11821 tttccattat taaccggtac ttgcgatttg cggataaggt ggaaggcaaa aggcgaacta
    11881 actggccctt gaatgaacaa tgggggcgct tcattggacg gaatcagaag gaaattcaat
    11941 taacttctga accgaagcct tataccattg aacgaacgtt gaactggctt ggacgacaag
    12001 tcgcccctac gtggaagatg gccaaggagt tggatcggtt gaatcagaca acgtacattc
    12061 aggatatggt aaataatact cgactatcag accggcacaa aaagattttg gagcaacaaa
    12121 gtatggcaat tgaaaatttg attatatgaa aagaggaatc gtgatggaaa ttgtgaaaga
    12181 tattttattg gtgtttggtg gcagtatgct tggtgttact gtgatgtgct tgatgcatgc
    12241 aagtgcagcg gcggaccgag caatggaaaa gaaaggaaag tgatgaaatg aatttcggtc
    12301 aaaattt
//
LOCUS       DAPCMT010000086        12132 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPCMT010000086 DAPCMT010000000
VERSION     DAPCMT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.088"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(85..642)
                     /gene="frr"
                     /locus_tag="RD743_001910"
     CDS             complement(85..642)
                     /gene="frr"
                     /locus_tag="RD743_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDU0563715.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(645..1367)
                     /locus_tag="RD743_001911"
     CDS             complement(645..1367)
                     /locus_tag="RD743_001911"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDU0563716.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1503..2384)
                     /locus_tag="RD743_001912"
     CDS             complement(1503..2384)
                     /locus_tag="RD743_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDU0563717.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2483..3265)
                     /gene="rpsB"
                     /locus_tag="RD743_001913"
     CDS             complement(2483..3265)
                     /gene="rpsB"
                     /locus_tag="RD743_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDU0563718.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3624..4103
                     /locus_tag="RD743_001914"
     CDS             3624..4103
                     /locus_tag="RD743_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDU0563719.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4320..5411
                     /gene="phnW"
                     /locus_tag="RD743_001915"
     CDS             4320..5411
                     /gene="phnW"
                     /locus_tag="RD743_001915"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDU0563720.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5404..5532
                     /locus_tag="RD743_001916"
     CDS             5404..5532
                     /locus_tag="RD743_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDU0563721.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5536..6081
                     /locus_tag="RD743_001917"
     CDS             5536..6081
                     /locus_tag="RD743_001917"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDU0563722.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6535..8226)
                     /locus_tag="RD743_001918"
     CDS             complement(6535..8226)
                     /locus_tag="RD743_001918"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDU0563723.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8646..9593)
                     /gene="arcC"
                     /locus_tag="RD743_001919"
     CDS             complement(8646..9593)
                     /gene="arcC"
                     /locus_tag="RD743_001919"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDU0563724.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9708..10727)
                     /gene="argF"
                     /locus_tag="RD743_001920"
     CDS             complement(9708..10727)
                     /gene="argF"
                     /locus_tag="RD743_001920"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDU0563725.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10818..12047)
                     /gene="arcA"
                     /locus_tag="RD743_001921"
     CDS             complement(10818..12047)
                     /gene="arcA"
                     /locus_tag="RD743_001921"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDU0563726.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3316 a         2519 c         2198 g         4099 t
ORIGIN      
        1 ttcttttttt aatttttaaa taaaagaaat ataaataaaa acagaggaag cggaatgatc
       61 cgcccctctg tttttatgat tttattaaac ttccaataat tctttttctt tatcagcagc
      121 gatcttatct acttctttga tgctgttatc tgtcaatgtt tgtacgtctt tttctaatcc
      181 gcgcaaatca tcttcggtaa tatcaccatt tttttgttgt tttttatatt catcgatcgc
      241 atcacgacgg atgttacgaa tcgcgatttt tgcattttct gcttcttttt taacatcttt
      301 agctaattct tttctgcgtt cttctgtcag ttgcgggata actaagcgaa tgacatttcc
      361 atcattggtt ggactgattc cgatatcact tgccataata gctttttcaa catcttgaag
      421 aatgctcttg tcaaaaggtg tgatcattaa aacacgtgct tctggaattt ggattgatgc
      481 catttggttc aatggtgttg gcgcaccgta gtagttcaca gtaatacggt ccaaaaggct
      541 agcgttcgca cggcctgcgc ggatttgccc aagttcacgt tgaagatttt gtgctgcttt
      601 tttcattttt tcttttgctt ctgtcataat tgcatctgcc atttttattt cccccttaca
      661 gttgttccga tattttcgcc taagattgca cggcgaatat ttccgtgttc atttaaattg
      721 aagacaagca gtggaatatc attatccatg cttagtgaac ttgcagttga atccatgact
      781 tgtaatcctt tggcaataac ttccagatga gtcagttctt caaattttac tgcatttgca
      841 tctactttcg gatcagcaga ataaacaccg tctacattgt ttttagccat caggataaca
      901 tctgcgccga tttctgccgc gcgtaatgct gctgttgtgt ctgtcgagaa gtacgggttg
      961 ccggtaccgc ctgcaaagat aacgatccgt cctttttcta aatgacgttc tgctttacgg
     1021 cgaatatatg gctctgcgat ttgacgcatt tcaatcgaag tttgcacgcg tgtcggtaca
     1081 cccacgtttt ccaacgtatc ctgtaaagct aaggcattca taactgtagc taacataccc
     1141 atgtaatcag cttgagcacg ttccatgccc atttgtgctc cgatttgtcc gcgccagata
     1201 tttccgccgc ccacaacgat tgccatttca acgcccagct cgtgaacttc tttgatttct
     1261 tggatgattt ctttgattac tggtggtttg attccaaatc ctgcgtcccc agctaaagct
     1321 tctccactca attttaagac tacacgctga tatttcggtg tgaccatgtt cattcctcca
     1381 atattttcta ttgccatttt accatactta gatgatttcg acactctaag actggttttt
     1441 cttgttttgc ttaaaaaaag ggagcgcaca aaccgtgcgt tcccgtctgt tattcaacag
     1501 aattattttt tcacttggct cataacttct tctacaaagt tatcttcgcg tttttcgata
     1561 ccttcgccaa cttcgaaacg tacgaatgtt ttaacagttg cgccttttga tgcaacgtat
     1621 ttttcaacag tcatatcagg atctttaacg aatggttggt ccaccaaagc gatttctgct
     1681 ttgaatttct tcaagcgtcc ttctaccatt ttttcaacga tattcgctgg tttgccttcg
     1741 ttcaatgctt gttctgtaag aacagttttt tcatgttcta attcagcttc tggaatttgt
     1801 gattcgttta cataacgagg attgatagct gctacgtgca tagctacgtc tcttgcaact
     1861 gtttcgtcag ttgttccatc tagaacagtc aatacggcaa tacgtccgcc catatgcaag
     1921 tatccaccaa atgcagcatt gtcgtctttt tctacaactt caaagcgacg gaagctgatt
     1981 ttttctccga taacttgagt tgcttcgatc aagtcagatt caattgtacc tttatctgtt
     2041 ttgattttca ttgcagcttc catgtctgct ggtttgtttt cagcaacaag ttctgcaatt
     2101 tcttttacta aatcttggaa catttcgttt ttagaaacaa agtctgtttc tgagtttact
     2161 tcaacgattg ctgcagtgtt acctttcaca gcaactgaag ccaatccttc agctgcaatg
     2221 cggtcatttt tcttcgcagc ctttgccatt cctttttcac gtaaaagatc aactgctttt
     2281 tccatgtcgc cttctacttc aactaatgct tttttcgcat ccatcattcc tacgccagtc
     2341 atttcgcgta gttcttttac cattttagct gtaacgtctg ccatgtttaa ctcctcctgt
     2401 gtgtttttct ttttaaaaaa agctgcttca aaggagagtg aggcttgtcg cccagccttt
     2461 gaaacagctc catcattcaa aattattctg ctgaagaatt gtcgccttca acaacatcta
     2521 cgatttcttc gattgatgtt gctgtgtttt cttctgtgaa atcttcttca actacttgat
     2581 cttcaccttg gtttccttcg atgaaagcat cagccatttt tgaagtaatc aatttaacgg
     2641 cacgaattgc atcgtcgttt gaagggatta ctacgtcgat ttcatctgga tcgcagtttg
     2701 tatcaaccat agctacgatt gggatattta atttgtgtgc ttcttgaaca gcaatacgtt
     2761 ctttacgtgg gtcaacgatg tacattacat ctgggattct tggcatatca gcgataccgc
     2821 ctaagaattt ttcaagacgt tcacgttctt tgttcaaacc agcaacttct tttttaggta
     2881 gaacttcaaa agttccatct tcttccattg cattgatttg tttcaaacgg ctgatacgtt
     2941 tttggatagt atcccagttt gtcaatgttc cgcctaacca acgatggtta acgaagtatt
     3001 gtcctgcacg agttgcttct tctttgattg cttcttgtgc ttgttttttt gtacctacga
     3061 ataatgctac gccgccttct tcagcaacgc tcttcatgta atcataagct gcatctacta
     3121 atttaactgt tttttgcaag tcaatgatgt agattccgtt tctttctgtg aagatatatt
     3181 tcttcatttt tggattccag cgacgagttt ggtgaccaaa gtgtacgccg gcttctagta
     3241 attgtttcat agaaattact gccatgtttg tttcctccga tttggtttta tttttcctct
     3301 tcttatcttc taccttgccg gcaaactttt tcaagcacct accgacaatc caataagaat
     3361 gtggatttgc tgatgcataa agcaccctca gctattatac agacaaaaga tccgtatcgc
     3421 aagagcaaat atacttttct gctcaacatt caatagttta cctgaaagat aaaaagaatt
     3481 caagaaaaac agtagatttc tctttgtttc tttattatcc tttttccata aaataatttt
     3541 aggtataaaa gacgaatatt attgggcaat ccttcttttt tttgttatga tacatgtgaa
     3601 atctttcgta aaggagaaag aaaatgcgca aagctcaaag acatttgctg attaaacaag
     3661 tcattgaaga attcccgatc cgtacgcaag aagaactatt aaacaaactt gggcattatg
     3721 gcgttaccgc cacgcaggca acgatttctc gagatatccg tgatctaaaa atcgtgaagg
     3781 cacctgatga aaatggcgtt tcccgcttcg tattgtttcg cgaaaaagga actcctgaga
     3841 ctaggactga ggaagaggat cggctggttc aaatgattga agacattgta ttgaaagtcg
     3901 aacgagtcca ctttttgacg atcgtcaata ccttgccaga caatgctcat ttatttgcat
     3961 ctgttttaga tgagatcaaa cctccctcta ttgtcagtac aattgctgga ttcgatacga
     4021 tcatggtcat ctcggaaaat gaggaggacg caaaacgagt agaagaattt cttcaccatc
     4081 caactgaagt atcacttttt taatcagagc acgtattcct aatatggaat gcgtgttttt
     4141 ttattttcaa tattttcctt attgaataaa cctttacact ctttacaaaa atacatcatt
     4201 ttttattcaa atttttcaac accttgattg aatcacaata atcatcattt atattggatg
     4261 ttaacaagag cagcttcttg ctctttgtgt atttcaaaaa agaaatgaaa ggaaatcata
     4321 tgtataaatt attaacttct ggaccattga cgacttctga aacagtgaaa aatgctatgc
     4381 tgatcgatca ttgtacttgg gatcaagaat acaaagaaat tacacaatgg ataagaaaaa
     4441 aattattgga tcttgctcaa gtatcggaag gaatatacac aacggtctta atgcaaggta
     4501 gcagctcctt cggggtagaa tcggtattaa gttctgtgat caggccagaa gatactgtac
     4561 tgatttgttc aaacggcgca tatagacaac gaatgatcaa catgtgcgaa tgtctgaaac
     4621 tgaactatac cgtttatgag gtatcggaac atcaaatcct ccaagctcat gtgattacac
     4681 agctgattga agagaatccg tctatctccg cactagcaat ggtccatagc gagactactt
     4741 ccggtatttt gaataatttg gaagagattg ctgctgtcac acaagaaaga gatcttttgt
     4801 ttatagtgga cgcgatgtca agctttggtg ggataccgat ttgtgtagcc gaactaggga
     4861 ttgatttttt gatttccagc tcaaacaaat gtattcaagg tgttccaggc ttttcttttg
     4921 tacttgctaa taaagcggaa ctggaaaaac gaaaaaatgt tagccgaagt ttgtcacttg
     4981 atctatatga ccagtaccgc gtgatggaaa acgagaatgg caaatggcgt ttcacttccc
     5041 ctacccatac ggttttggct tttcaacaag cgctgaagga actagaggca gaaggaggaa
     5101 tcgaaactcg atattcacgt tatctcgcca ataatcatgt gctacgacaa aaaatgacag
     5161 agatggggtt ccagcctttc attcaagaat atcaggggcc attcatcacc tcttttcttt
     5221 accctaagga ctggcgattt gattttaatc atttttatca gtatttaaag caaaatggat
     5281 ttgtgatcta tcccgggaaa atcagcgatc atgaatgttt tcgtatcgga aatattggcg
     5341 agatctatcc ggaagatatt ttggaactga cagcaattat caaagactat aaggagaacg
     5401 ttaatgatta aaactgtaat ttttgattgg gcaggaacaa ctgtggactt tggctgtatg
     5461 gctcctgtcc acgcttttag aaatgctttt ctagaaaaag gaatccaact gactgacaaa
     5521 gaaatccgct aaccgatggg aaaattaaaa tgggatcata tccaacagct tctttccctc
     5581 ccttctgtca aaaaacaatg gatagctatt tatggacaac tacctcagga aaaagaggtg
     5641 gaggaattgt atcaacaatt tgaacattat ctgtttaaag atttggtcaa gcattctacc
     5701 ttgaaacccg acacattgga aacgatcgaa cggttgagaa acgctgggat caatattggt
     5761 tccacgaatg gatttaatgc tcctatgatg acaatcgtag cagaaaccgc aaaaaaagaa
     5821 aggtattcac cagacttcat ggcaactttt gaagacgtcg aagggtatgg tcgaccgttt
     5881 ccttatatga tccataaaaa catgcagtac ttcaaaaaca agtcggttga tgaagtaatc
     5941 aaagtcggag atacggtctc agatatccag gaagggaaaa atgccggagt gttgacagta
     6001 ggtgtaattg aaggtagttc gctaatgggg atttcttatg atgagtacca aaaattagag
     6061 atggggaaaa agatcgttta aaacagtctg ttgctcttag attccaacaa gccggcgctg
     6121 atatttgtgt aaacaattta aaagagttaa cagaatacat tttgtacgct gacatgaatc
     6181 cagaatgact tttatacaaa ataagaggtt gtgaaaaagc tgtcctgtat caagtaataa
     6241 aaacagccaa gcaaaaatag ctcctcatat ttttcgtttg gttaggctta ttaccgcaga
     6301 tgcaagcttt tgaacaccgt ttattcgaaa aaaaaaaggg agctgtgaca cgacttttgt
     6361 cacagtcccc ttatttgcaa acaaacagtg tttaagagac aacctctcca ctaaacggtg
     6421 ttcattcagc tgactctcgg tatttgctca aaaaacgata aaatttgaaa agctattttc
     6481 tcttttgttt ctctaatact cggatcagaa cgctgatttc acattctctt tttcttacat
     6541 cttatctggt gcatgaagtc ctagcaaacg aaggtcttct ttcaataata cagtcactgc
     6601 ataaactaga gctaagcgtg cttctttttg ttcatcatca gctaaaatct tcgtatgagc
     6661 atagtatttg ttgaatgctt gtgctaattt gattgcatgt ttcgcaatca ctgacggttc
     6721 gtatttttcc ccagcagaca agaccgtttc agggtatttt tgaactaatt taaccacttc
     6781 ccagctggta tcgtcattca atgcatagtc tgctgtttct gaagggacaa atcctgcttt
     6841 ttccaacagg ctgactgcac gagcatgtgt atattgcaca taaggtccag tttctccttc
     6901 aaatcgaaca acttcttcca ggttaaagtc aaatgtattc agtcgatcgt ttttcaaatc
     6961 gtggaaaatg actgccccaa ccccaacttg tttagctaca gcatctttgt tttccaaatc
     7021 agggtttttc tcgctgattt gttctttagc agattggatt gcttcattta atacttcttc
     7081 taataagacg atctttcctt tacgagttga taatttttta ccaccttgcg tgatcaaacc
     7141 aaatggaata tggtgcatgt cgtcagacca gtcaaagccc atttccttca agacagcttt
     7201 caactgttta aaatgatagc tttgttcatt ccctactaca tatagagatt gtttgaagtc
     7261 gtaagtacgt ttacggtaca atgcagcagc taagtcacgt gtaatgtata gtgtcgcacc
     7321 gtctgacttc ttaatcaacg ctggattcag gtcataagca gataggtcaa cgatctctgc
     7381 acctttatcc tcgttcagca aatgtttttc ttctaaaagt ttgacgattt catccatctt
     7441 gtcattgtag aaagcttcac cgttcaacga atcgaaacgt acttccagca gatcatagat
     7501 tttattgaat tctttcatcg attcatcacg gaaccattgc cataattgaa tagcctcttt
     7561 atcgccttct tccagacgtt tgaaccaagc acgagcttct tcatttaatt ctggttctgt
     7621 ttcagctact tcatggaatt ggacgtataa acgaagcaat tcattgattg gttctgcttt
     7681 aacagcttct tctgtacccc attttttgta agcgacgatc agttttccaa actgtgttcc
     7741 ccagtcacct aaatggttga tgcggattgg ctgataaccg attttttcca taatgaaacc
     7801 gatcgaatta ccgataactg tcgaacgcaa atgtcccatt gagattggtt tggcaatatt
     7861 aggagaagac atgtcgattg gtactgttcc ttgattcccg atgttgctat ccccataatg
     7921 ttctttttct ttcactactg tttgcaaaac ttttttgctg ataagttctt tattcatgaa
     7981 aaagttcaag taaggtccga caacttcgat tttttcaaag ttggcagaat cgattttttc
     8041 tgctaaatct gcagcgattt gctgtggcgc ttttcggtaa acttttgcta atgaaaaggc
     8101 tggaaatgca acatctccgt gttcagctga ttttggattt tctagtaatt gctcgacttg
     8161 ttccagtgtt aaatcatctt tgacagcgtc atagacagct tttgcgacaa gatctttatt
     8221 attcatcttt attcctccta aagtgttccc ttgattgtta aacaaaaaaa atctctagcg
     8281 acggcatact tagtatgcca tcactagaga cgagatttcc cgcggtacca ctccaattgt
     8341 cctaaaaaag gaccagctcc atctgatatc ggtagacgac cgtctgttta aaacagaatt
     8401 ctccaagtgc gcttcgttgt atcttcttca ttcggcttcc accacttccg aattcgctag
     8461 ccaagaatga tacaagtact ctcttgttca acaaatctta gtcttattaa actttcttga
     8521 attatatcag aaaaaaaaac gaaaggctag tcgtttcata aaaaaacaat ccagatggcc
     8581 taaaggagaa gaaataccac ctggatagct ataaatcatt tcgtaaatac gaaagactta
     8641 acaaattaat cagctacgat aatcgttcct tcttcagaag ctaaaagatt ctcgatattt
     8701 tctaaagaag tgatgattgc cttagcgttt ggttttgctt caacaaatgc aatagctgct
     8761 tctactttcg gaagcatgct tcctggagcg aattgctttt catcaatgta ttgtttcatt
     8821 tctgaaacag taacagtttc taatttcttt tgatctggtt tttggtaatt gacatacaca
     8881 ttgtctactc ctgtcaatac gatcagcaaa tccgcatcga tgatttcggc taatttttct
     8941 gaagcaaaat ctttatcgat tacggcttct cgtccttcta atccagtagc tgtttctaca
     9001 acaggaatcc caccgccacc aacagagact gtgataactc cttgatcgac cagcgtttcg
     9061 atgacacgtg cttctttaat cgagatgggt ttaggggaag cgactacttt tctccatcca
     9121 cgtccagcat cttctttgaa tgtcacagtg gagtcagcat tcatttgttc tttggcttct
     9181 tcttccgtgt agaatggacc gactggttta cttggatttt tgaatgaagg atcattttca
     9241 tccacaataa cttgagtgac taaagaaacc acatctttat cgatcccttt ttccttcagt
     9301 acttcgccca tggcgttttg taaccagtaa ccgatagatc cttctgtcat tgcaacacat
     9361 gtatctaaag gcattgccgg tgttttctca gaatcagcag cttgctgctg aatcaatagg
     9421 ttaccgactt gcggaccatt tccgtgggag atgatcaatt catctccttg ttcgataaat
     9481 ttcactaaat actttgctgt ttccattaac gcttcttgtt gggcttttgc acttgcatcc
     9541 gtagacaaga tggcattgcc ccctaaagcg acaactactt ttcgtttggc catttcattc
     9601 gtcctttctt cagttctacc gaattttcta gataacaaaa cgagccagcg ttttgtgatt
     9661 ttccctcaat gctgactcgt tttttcgtga gatgaatgtg gttatgctta aacgcgaggg
     9721 ataaacaagt tacctaatgt tgctgccatg atcgctttga ttgagtgcat acgattttct
     9781 gcttggtcaa attgacgagc atatttgctg cggaagactt catctgttac ttccatttca
     9841 gtgatgccaa agcgttcttt catttgttca ccgtaaacag tgtttgtatc atggaaagct
     9901 ggcaagcagt gcaagaagat caaacgatct gtgttgtgtg ttttttctac catttccata
     9961 ttgatttggt aaggtttcaa caatttgata cgttcttcga atttatcttc ttcacccatt
    10021 gatacccata catcagaata aaggacgtct gctccgtcta cacctttatc aacatcgtcg
    10081 gtaatcatca attgtgcacc ggatttttca gcaaagcctt ctgccatttt gacgatttct
    10141 tcttcaggtt gtaattcttt tggtgcaacg atacgcatat tcgctcctaa aattgcacct
    10201 gtgactaata aagagttagc catattgttt cgaccatctc cgcagtaagc aacagtgata
    10261 ccttctactg ttccaaagtt ttcttggata gttaaaaagt cagcgatcat ttgtgttgga
    10321 tgccattcat cagtcaagcc attccaaact ggaacaccag agaattctgc tagttcctct
    10381 accatttttt gactgaatcc acggaattcg attccgtcaa acatacgtcc tagtacttta
    10441 gctgtatctt ctgtggattc ttttttacct aattggatat catttgcacc tagatattca
    10501 ggatgtgcac ctagatcgat cgctgcagta gtaaatgcag aacgagtacg tgtagatgtt
    10561 ttttcaaata aaagcgcaat attttttcct tctagatagt gatgtgggat accacgtttt
    10621 ttcaagtctt tcaagtgttc tgaaaaatcg atcaaatatt gtaattcttc ttttgtaaag
    10681 tctttttctg ctaataagct tcttccttgg aatactgatt ctttcatatt aaacattctc
    10741 ctttagattg tttttattta agattaaaga ggaaatcccg acggcagctg acagtaagac
    10801 tgccgtcagg aaacaaatta cttcaagtct tcacggacaa gtggttggct catgcagcgt
    10861 ggaccaccac gacctcgtga caattcactt gaattgattt ctaacacttt gatgccatag
    10921 ctacgaagca attcgttcga tacataattt cggttataag tgactacaac acctggagcg
    10981 attgccaatg tgtttgatcc atcattccat tgttctcgag gtgcaacgat ctcatctcca
    11041 ttaccagttg ggatcagtac aagatcatct aatccaagag cagctttcat cgtttcgtgt
    11101 aagttatctg aatgagtaat cttgatatca tcaccatctg gttcgattgt aaagacatcc
    11161 atcttaccgt ctttactttg aattgctgga tggatagtga atttatcgtg atctaccatt
    11221 gtaaatacgg tatccaaatg catcattgca cggttgttcg gaattttgat tgccaacact
    11281 ttttcaaatc ctgaattttt tgcgaatagg tggcgtgcta atttttctaa agctttcgca
    11341 ttagttcgtt gagaaatccc aacagctact actttatcgc ttaagatcaa ttcgtctccg
    11401 ccttcgatat gatctggatt ttcacgatcc aaccatactt cgattccttt atttgcaaat
    11461 cttggatgat gtttcaaaat atattctgta aacatcgatt cacgacgacg tgcttcaaaa
    11521 gtcatcttat tgactgtcat cccatttccc attgatgcgg aagggtcgcg tgtaaagtac
    11581 aagtttggca ttgggtccat gtaaaatgga tagtcatcgt ctgctgacag atcatataga
    11641 ctgctagaac gtacttcgat atcttttgta cgaaccccag ccatgatttt atctaccatt
    11701 tcttgcgttt ccattgatag aaggaactca tgaagaccct ctcttacagc attcgatgca
    11761 atatgagatt catccaacat tttattcaag aattgttctt tgacattgcc tgcgtcaatc
    11821 gcttctgccg ctaatttttc caagtaaaga gtctcgacgc cttcattttg caatgtcttc
    11881 gcaaagttgt catgttcttc ttgagcaatc ggaagatatg gaatatcatc gaacaacaga
    11941 cgatccataa tatctggtgt caaattttcc acttcttgtc caggccgttt cagtaaaact
    12001 gtctttaatt tccctatttc agagaaaacg tgaataggtt tatccatgtt gtgttgcctc
    12061 cttctttttg attacatgta taggatatcc tccttttgta atcactttca taattttgtc
    12121 tcattcaaaa tg
//
LOCUS       DAPCMT010000087        12063 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPCMT010000087 DAPCMT010000000
VERSION     DAPCMT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.089"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            24..266
                     /gene="yaaA"
                     /locus_tag="RD743_001922"
     CDS             24..266
                     /gene="yaaA"
                     /locus_tag="RD743_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDU0563727.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            253..1377
                     /gene="recF"
                     /locus_tag="RD743_001923"
     CDS             253..1377
                     /gene="recF"
                     /locus_tag="RD743_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDU0563728.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1374..3320
                     /gene="gyrB"
                     /locus_tag="RD743_001924"
     CDS             1374..3320
                     /gene="gyrB"
                     /locus_tag="RD743_001924"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDU0563729.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3340..5811
                     /gene="gyrA"
                     /locus_tag="RD743_001925"
     CDS             3340..5811
                     /gene="gyrA"
                     /locus_tag="RD743_001925"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDU0563730.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6047..6346
                     /locus_tag="RD743_001926"
     CDS             6047..6346
                     /locus_tag="RD743_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDU0563731.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6396..6908
                     /gene="ssb"
                     /locus_tag="RD743_001927"
     CDS             6396..6908
                     /gene="ssb"
                     /locus_tag="RD743_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDU0563732.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6934..7170
                     /locus_tag="RD743_001928"
     CDS             6934..7170
                     /locus_tag="RD743_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDU0563733.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7313..9286
                     /locus_tag="RD743_001929"
     CDS             7313..9286
                     /locus_tag="RD743_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDU0563734.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9292..9744
                     /gene="rplI"
                     /locus_tag="RD743_001930"
     CDS             9292..9744
                     /gene="rplI"
                     /locus_tag="RD743_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDU0563735.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10018..11385
                     /gene="dnaB"
                     /locus_tag="RD743_001931"
     CDS             10018..11385
                     /gene="dnaB"
                     /locus_tag="RD743_001931"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDU0563736.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         4022 a         2197 c         2661 g         3183 t
ORIGIN      
        1 ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga
       61 cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt
      121 atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac
      181 tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag
      241 gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag
      301 ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa
      361 acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt
      421 gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa
      481 ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc
      541 aaccatatcg aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt
      601 gcaccagaag atctttcttt agtaaaaggc agcccgcaga ccaggcggaa gtttttagat
      661 atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt
      721 ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt
      781 tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga
      841 gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat
      901 caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg
      961 caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt
     1021 tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag
     1081 aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa
     1141 ttagcagaaa tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat
     1201 gatgtgatga gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga
     1261 aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt
     1321 gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag
     1381 aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg
     1441 aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg
     1501 gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt
     1561 ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg
     1621 gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct
     1681 ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac
     1741 ttcacggagt aggatcttct gttgttaacg cgctttctac acagcttgat gtcaaagtat
     1801 acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa
     1861 agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa
     1921 tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg
     1981 ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca
     2041 tcaatgaata ccattatgac ggcgggatta aaagttatgt cgaatacttg aatgcaaaca
     2101 aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg
     2161 aagtctctat gcaatataca gacggctacc attccaatat catgagtttt gccaacaata
     2221 tccatacgta tgaaggcgga acacatgaat caggattcaa gactgcttta actcgtgtga
     2281 taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg
     2341 aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg
     2401 aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat
     2461 ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgt caaattgttg
     2521 aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga
     2581 gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca
     2641 acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta
     2701 agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg
     2761 ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg
     2821 ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag
     2881 ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt
     2941 tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt
     3001 tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag
     3061 aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt
     3121 ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca
     3181 atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa
     3241 tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg
     3301 tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa
     3361 aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg
     3421 agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc
     3481 cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct
     3541 gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacta tgcgatctat
     3601 gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat
     3661 ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg
     3721 atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa
     3781 agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt
     3841 ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac
     3901 ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat
     3961 gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt tatgggaaaa
     4021 tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt
     4081 gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg
     4141 gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa
     4201 gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc
     4261 cgtcgtgacg ccagcgcatc tgttatcttg aataacttat ataaattgac atccttgcaa
     4321 aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg
     4381 aaacaaattc ttgaaaacta tatcgaacat cagcgcgaag tcatcacaag acggacagtc
     4441 tttgaaaaag aaaaagcaga agcaagggca cacattttag aaggtttgcg tattgctttg
     4501 gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa
     4561 gcgacgatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg
     4621 cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc
     4681 ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc
     4741 atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta
     4801 ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt
     4861 attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa
     4921 cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat
     4981 ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat
     5041 cgggcaaaag gatacgaaat tccagaatac gggcgcacag ccaaaggaat accaattatc
     5101 aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa
     5161 gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct
     5221 gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac
     5281 gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat
     5341 aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc
     5401 ggggttagag gaatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag
     5461 gaagatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg
     5521 gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa
     5581 aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca
     5641 gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca
     5701 ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc
     5761 gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata gccatcaaga
     5821 ctaattgtgt aagctagcaa gaatgaccga ttaaaaaatc tggttattcc tgctggcttt
     5881 tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac
     5941 caaaatagag tgtataatca gagcttgtga gtagtctaat taattagata actctccttg
     6001 ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa
     6061 atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat
     6121 cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg
     6181 ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt
     6241 taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat
     6301 caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt
     6361 gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg
     6421 gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat
     6481 ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta
     6541 tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa
     6601 cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac
     6661 gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat
     6721 ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg
     6781 gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc
     6841 gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac
     6901 cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt
     6961 aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat
     7021 atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact
     7081 ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg
     7141 ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt
     7201 ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc
     7261 gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa
     7321 taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt
     7381 attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa
     7441 tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga
     7501 aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat
     7561 gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt
     7621 agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt
     7681 tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga
     7741 tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa
     7801 actccatcag acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga
     7861 tgccattgat aaattggatg acaaagagat ttcttatttg aactctttcg tcactacact
     7921 agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt
     7981 tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct
     8041 ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat
     8101 tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt
     8161 tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc
     8221 tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc
     8281 gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca
     8341 caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt
     8401 caaccagaaa aaagcttaca tcgttatcaa cgagaacgag atcattccag atgtagaacg
     8461 gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg
     8521 tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc
     8581 tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca
     8641 tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatatcgaat cttcagcttc
     8701 ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt
     8761 agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa
     8821 tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga
     8881 cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa
     8941 tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga
     9001 aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa
     9061 tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag
     9121 aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt
     9181 cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt
     9241 tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta
     9301 atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca
     9361 ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt
     9421 atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa
     9481 gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg
     9541 aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg
     9601 ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc
     9661 cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc
     9721 aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa
     9781 ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca
     9841 aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca
     9901 taaagaaaac gagtataata aaagagaact atttttttat tacactttcg gtacgaatga
     9961 tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg
    10021 aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta
    10081 ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa
    10141 gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt
    10201 aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa
    10261 gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat
    10321 atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca
    10381 gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat
    10441 gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct
    10501 attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa
    10561 gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag
    10621 cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg
    10681 aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa
    10741 atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc
    10801 catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg
    10861 agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc
    10921 cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat
    10981 tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc
    11041 tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag
    11101 ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa
    11161 tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat
    11221 gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa
    11281 gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa
    11341 gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag
    11401 ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta
    11461 gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct
    11521 ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa
    11581 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac
    11641 gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca
    11701 tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc
    11761 tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca
    11821 aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc
    11881 tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa
    11941 atttcacaaa aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc
    12001 acttctgtta caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag
    12061 cgt
//
LOCUS       DAPCMT010000088        11691 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPCMT010000088 DAPCMT010000000
VERSION     DAPCMT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.090"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(123..608)
                     /locus_tag="RD743_001932"
     CDS             complement(123..608)
                     /locus_tag="RD743_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDU0563737.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    698..1043
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1088..1729
                     /locus_tag="RD743_001933"
     CDS             1088..1729
                     /locus_tag="RD743_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDU0563738.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1748..2383
                     /locus_tag="RD743_001934"
     CDS             1748..2383
                     /locus_tag="RD743_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDU0563739.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2401..3216
                     /locus_tag="RD743_001935"
     CDS             2401..3216
                     /locus_tag="RD743_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0563740.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3535..4971
                     /locus_tag="RD743_001936"
     CDS             3535..4971
                     /locus_tag="RD743_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0563741.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5160..7607
                     /locus_tag="RD743_001937"
     CDS             5160..7607
                     /locus_tag="RD743_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDU0563742.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7837..8904
                     /gene="rlmN"
                     /locus_tag="RD743_001938"
     CDS             7837..8904
                     /gene="rlmN"
                     /locus_tag="RD743_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDU0563743.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9097..10200)
                     /locus_tag="RD743_001939"
     CDS             complement(9097..10200)
                     /locus_tag="RD743_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDU0563744.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10753..11301
                     /gene="aac(6')"
                     /locus_tag="RD743_001940"
     CDS             10753..11301
                     /gene="aac(6')"
                     /locus_tag="RD743_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDU0563745.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3874 a         1971 c         2518 g         3328 t
ORIGIN      
        1 ttagttttca gcgttcatac tagttgttac attgtttgat tggccaaaag aatttctgat
       61 atattttgtg ctaaataaag aaaaagacgc ttgaattggg aaataagaaa actactctat
      121 cattacaaat tttcataaac tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg
      181 gaaaaatcca taatcaacgt agtggtattg atatttggat gaaagcctac ggtatcttga
      241 cgatcaatag cagcatcgat cacgacttgg atttttttct ctttgtcgtg catcaatgct
      301 aatggagtca ctgtacctgc tggaacgcct aggaggtcag gaagctgttc ttcagaaaca
      361 aaagataagc gcttttcttt taattgttca gctagttgtt ttaaatcggc gtgcttttca
      421 tcaggcaaga tgacaagata gatttgtttt ccttttcttg atttcaataa tagatttttt
      481 acttgcggac caggtaggga aaaagggaca tttttgactg acgtaatcgc aggatgatcg
      541 actttttgaa aagagatacc cagttttttt agtagttcat aagcttcttg ttcggttgca
      601 cgactcatcg cgcatcaccc tccttttttt attagtgtat cataaaaaag agttgacaaa
      661 tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga aaagaagagt
      721 agccgttagg tgtttttaga gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga
      781 aacacatctt ggagtcgatt ttctgaagcg tatagtagga aaatccgggg agcccgttac
      841 agctgcggtc gttgctagat gaccagataa ggctggcttt tccgtgagga gtcggtaaaa
      901 aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga tcaaaggact ttttgttttc
      961 atctgaacgg caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga
     1021 accacgtata atacgtcctc ttggcatttg ccaagaggat ttttttattt taaaggagga
     1081 aaagaagatg gagtatttga ttgaagttat gccgcaatta ttagtaggag cactcacaac
     1141 attgaaatta tttttattta ccttgattgg ctcattacca ttagggattg ttttagcatt
     1201 aggattgtct ttgcatttta agccgctacg ctacttgctt caaacatatg tatgggttat
     1261 gcggggaaca ccgctgttgt tgcaactgat ttttattttt tatgggttgc cgacgattgg
     1321 gatcgttttt gatcgtttcg aagcagcact cattgccttt atcttaaact atgcagctta
     1381 ttttgcagag atctttcgtg gagggataca atcgattcct caaggtcaat atgaagcagc
     1441 gcaagttttg caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac
     1501 aaagattgtt ttaccttctg tagggaatga agttatcaac ttggtcaaag attcttcctt
     1561 gatctatata ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga
     1621 cgcgacttta ctgcctttgc taggcgtggc aatcatctat cttacattga ctggtattct
     1681 aacaatcata atgaaacaag ttgaaggaaa attaaattat taccgctaac tgataggagg
     1741 aagaaatatg attgaattga agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa
     1801 agatttgaat ctaatcattc cagatgggaa aacattagct atcgtaggtc cttccggtgg
     1861 cggtaaaacc acgcttttac gtatcttggc tggcttagaa agttctgaca gcggagaatt
     1921 ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac aagttgtagg
     1981 agtcgtattc caagacttcc agctatttcc acatctgaat atttttgaaa acattacctt
     2041 agcaccaaaa atggtattga aacagacaaa agaagaatac accaaaaaag cagaagaatt
     2101 agcggcttca ttagggatta gtgatttatt gaatatctat ccttatcagc tttctggcgg
     2161 acaaaaacag cgggtagcga ttgccagagc attggctatg aatccacggg tattggctta
     2221 tgatgaacca acaagtgctt tagatcctga attacgccag caagtcgagc gtttgatcct
     2281 atcattgaaa gaagatgggg taacccaaat tattgtcacc catgatttag tatttgcaga
     2341 aaatgtcggt gatcaattac taaaagtttc cccaattcat taagaaagga gaagatagag
     2401 atgaaaaaaa ccagttttct attcgctttg atcgcaggtc ttcttctttt tgcaggctgt
     2461 tcgaacaaaa aaacttcagc agataattgg gcgactatca acgaagagaa aaaaatcgtt
     2521 ataggtttag acgatacatt tgttcctatg ggatttagag ataaagacgg gaagctcaca
     2581 ggatttgata ttgatttagc caaagcagtc tttgaacaat atgggattac ggtagacttt
     2641 cagcccattg actggtcaat gaaggaattt gaactgaaca atggaacgat cgatttgata
     2701 tggaacggct attccaaaac gccggcaaga gaaaagaaag tgcaatttac aaaaccttat
     2761 atggaaaacg atcaagtgct gatcacacct aaagaatcag gaatcacaaa ctttggacaa
     2821 atgaataata agagattagg tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa
     2881 caaccagaag tattaaaaga gatagtcaaa aaccaagatg ctgttttgta tgatagtttt
     2941 aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat ggataaagta
     3001 tacgcagact attatttgaa acaaagaaac gaactagaag acttcaacat aacaaaaggt
     3061 tcttatgaga gtgaggattt tgcagttggt gcacgcaagt cagatagtac attagtgaaa
     3121 aaaatcaatg aagccctaga tcaactagag aaaacaggtg aattccaaaa aatatccgat
     3181 aaatggtttg gcgaagatgt tacgccgaga aattaacaaa taggggactg tgacaaaaga
     3241 cgtgtcacag tccctttttc cgaataaaca gtgttctcct gccaactcct tgtgactcgg
     3301 agctgaatgg caggctcaca acctcttggt aaacggtgtt caaaagccag tttttcggta
     3361 ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt cttactactc
     3421 gaaactaagc gactttttca caacctcctt tacataattt tattttgttg tggaaggggt
     3481 ttcgtgttat tctaaaagag gaacgattga aggttatagg aaaggaagag atgaatggaa
     3541 cacaaagaac ttacctcatt tccaaaagat tttctgtggg gctctgcttc tgcagcgtat
     3601 caagtagaag gagcttggca agaagatggg aaaggagaat cagtctggga tcgatttgta
     3661 cggatacctg gaaagacatt taaaggaacg aacggtgatt tggcagtaga ccactatcat
     3721 cgttataaag aagatattgc tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct
     3781 gtagcttgga cacggatttt tcctaatgga cgaggagaag tcaatcaagc tgggctcgct
     3841 ttttatgaga gattgatcga cgaattgatt gaaaacgata ttgaacctat gttgacttta
     3901 tatcattggg atcttccaca agcattgcaa gatgaatata atggttggga atcacgtcag
     3961 atcattgaag atttcactca ttatgcagaa acattatttg aggcattccg cgggaaagtc
     4021 cattactgga ttagtttgaa tgaacaaaat atcttcactt cattaggtta tttgttagca
     4081 gcacatccgc caggtgtgac agacccgaaa cgaatgtatg aagttaacca tattgctaat
     4141 ttagcaaatg catctgtcat caataaattt cacgagttag aaatgcctgg aaaaatcgga
     4201 ccaagctttg cgtatacgcc aaattatcca attgacagta atcctgaaaa tgttctggct
     4261 gctgaaaatg cagaagacct gatggccaat tattggatgg atgtctatat gtggggaaaa
     4321 tatccgattg ctgccatgag gttcttagaa gaaaaaggat gggcaccaac gattgaagct
     4381 ggcgatgcag aattgctaga atctgctaaa ccagattttc ttgggatcaa ttactatcaa
     4441 acggcgacga atgcattcaa tccattaaac ggagttggtg ctggaaaaat gaatacgaca
     4501 ggaaaaaaag gttcatcaga agaaactgga gttcctggaa tgtataaaaa agttgagaat
     4561 ccatttgtcg aacgaacaaa ttgggactgg gagattgacc cagaaggatt aaggatcgga
     4621 ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga ttacggaaaa tggcttagga
     4681 gagtatgaca agttgacaga agataaacaa atccatgacg actatcgaat caattactta
     4741 caaagccacg tcaaagccat caaagaagca atttcggatg gagcagaagt cctaggctat
     4801 tgcacctggt cttatacaga tttgcttagc tggctgaacg gttatcaaaa gcgatatgga
     4861 tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt tggaacgata taagaaagac
     4921 agttattact ggtaccaaaa actgattgaa gaaaatggag agaaccttta gttcacatac
     4981 ttaaaacaag aaggtacggc tggatatggc cgtgcctttt cttgataaaa tcatttagaa
     5041 attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa
     5101 aactttagtt cccttacttt tttttgtata atgaaacttg atgaaatgag ggataataaa
     5161 tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa
     5221 aacagcagca ggaacaaatg ctgacgattg gtgttgggtt tgccttgatt ttatttgctg
     5281 tcttcggctt tttaaaatta ggttttttag gaatattgat tgcaaacggc tttcggatca
     5341 ttgcaggaaa tacttaccaa atcctctgtc ttttattagc cgtattagga ttttggattg
     5401 tcattaagaa tacagagttt tcaattggta aaaaccgacg ctggtttggc ggcatactct
     5461 tttatttcgg tatcttatta ttgttacatg cccatttatt tggcaaactc catactgggg
     5521 aaccaaatat catggggacg acatgggatt tgttagccag tgatataaaa caaagtcaag
     5581 tagacaataa cgttggtggt gggatgattg gagcaatcct ttatcatttt acttacttct
     5641 tgattgcaca gcctggaagt tatttagcag cagtgctatt actggcagga ggagcattcc
     5701 tgttcagtaa tctggaaggc tatcagctgt taaatggtat ccaatcaatt ggagaacgag
     5761 tacaggaatt attggaaggt gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag
     5821 agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg cttagctgca aaagaagctg
     5881 cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt gagccagcaa agggaaaata
     5941 ctgctgatga atggcaggaa ccagaaaata cagagccgga gcagctaagt tttgttccaa
     6001 tcgacagttt tcaagaaaat atccatcctg cgaatctaga gaaaccagta ccagatacac
     6061 caaaacagac caatacagca gaaggttttt cagatgagtt gccagaggat gacggtacct
     6121 cattagaatt tgagattgaa gcagaacagg aaaatcaaga ctatgaatta ccgacagttg
     6181 atttgcttga ttctatacca actgtggatc aaagtgatga gtataaaaaa attgaaaaaa
     6241 atatcggcgt attagaacaa actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag
     6301 ccagcttagg accatccgtc acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa
     6361 gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga
     6421 tggaagcacc gattccaggt aaatcactga ttggtattga agtaccaaat ggaaagatca
     6481 gtatggtttc ctttagagaa atcatcgaag cacaaccaaa ccacccagat aaattactag
     6541 aagtaccttt aggacgtgat gtttctggac gggtacaaac ggctgatctt tcgaagatgc
     6601 ctcacttact cgtggctggt tcgactggta gtgggaagtc agtagcaatt aatgggatta
     6661 tcacaagtat attaatgcgt gctaagcccc atgaagtaaa actgatgatg atcgatccga
     6721 aaatggttga attgaatatg tacaacggca tacctcatct gttgacgccg gtcgtgacga
     6781 accctagaaa agcagcgcaa gccttacaaa aagtagtaca agaaatggaa gaacgatatg
     6841 aaaaattcgc ggcaaccgga gtaagaaata tttctggtta caatgaattt gttcaacaga
     6901 aaaacttgga aaacggaacg aaacatccaa ccttaccatt catcgtagtg atcgtagatg
     6961 aattagctga tttgatgatg gtagccagca atgaagttga agatgcgatt attcgtttag
     7021 cgcaaatggc tcgggcagcg gggattcata tgatcctagc tactcaacgt ccaagtgtgg
     7081 atgttatcac aggaatcatc aaagcaaatg taccttccag aatggcattt gcggtatcaa
     7141 gcggtacaga ttctcggacg atcattgatt ctaatggggc agaaaagctc ttagggcgag
     7201 gagatatgct attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca
     7261 tttctgacca cgaagtagaa cgggtcgttc aatttgttac agatcagcaa gaagctcatt
     7321 atgaagagaa aatgatgccg acagatgaag ttgaaacgac aggtgctcca gaacagccac
     7381 aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt
     7441 cccttctcca aagaagattt cggatcggtt acaaccgagc agcacgttta gtagatgaac
     7501 tggaagctca tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg
     7561 agcaggaaga aacatcagaa acgttgccgg acgataatga catgtaaaac ttgtgtgatg
     7621 aagggttgaa aaacgaataa ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa
     7681 tggttttttt ccatcttttc taatacggta tgatgtcttg tctttttagt ttttgtgaga
     7741 ttaaagttct attcgattta tttgtgaagt attgcattaa ggaaatgaat catgtatagt
     7801 atgtagcgaa actttgagaa aaagagggaa aatactttgg aaaaacagtc catttatggc
     7861 ttgacgaatg aagaattaat caattggttt atagaaaacg gcgagaaaaa attccgagct
     7921 gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat
     7981 ctatcgaaac aattgatcga aaaattatcg gagcatttta tcatcaatcc tctaaaacag
     8041 atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt tgtttgaact ccctgataaa
     8101 aacatgattg agactgtact gatgagacaa gaatatggct tgtcagtttg tgtgacgaca
     8161 caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt
     8221 gatctgactg ctggagaaat tgttgcacag atcatgatgg tccaacatta ttttgatgag
     8281 cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga gccatttgat
     8341 aattacgaca atgtaatgga ctttttgcat atcatcaatg atgccaaagg attggcaatt
     8401 ggtgctcggc acattacagt ttccactagt ggattagcac acaaaatcaa ggaattcgct
     8461 aataacggct tgcaagtcaa tctggctatt tccttgcacg cgccgaataa tgaggtgcga
     8521 acatcgatca tgcggatcaa ccgaagtttc ccaattgaga aattgatgga agctgtggat
     8581 gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta
     8641 aatgatagac cagagcatgc gcaacaatta gcagatctat taaaagataa gaaaaaatta
     8701 gcatatgtca acttgattcc atataatcca gtcagtgaac atgatcaata ttctcgcagt
     8761 ccaaaagctg atgtattgag attttatgat gtattgaaga aaaacggcgt aaactgtgtg
     8821 atacgtaaag aacatggtac agatattgat gcagcttgcg gacaattgcg aagcaagcaa
     8881 atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt ttttcaatta ccataaaagg
     8941 agcagttttc ttaattaagg aactgttcaa cttaaataag agaaaagatt ctatagcgaa
     9001 agctataaag ctttttttga caagaaaaac cctcatcaag caaagaaatg tcctcaattt
     9061 gatgacagcc tgcttgatgg gggttttagc aattatttaa ctgattttct tatatccttg
     9121 ctggctttct tgtgtagcat gataagtttc tacgtcaaga atggcctgtt taaatttttc
     9181 tcgggtgatt tcataaggca catgtttaat atcatttgtt gccatggctt tatctaagat
     9241 tgcttctaaa tcggtcgtga cattgagatt catgtcggtt aacgatactg gaagttggtt
     9301 ctttttcata aacacataga ttttatcaaa caaatcgaac tgctgatcca tcatcaataa
     9361 ggtcagtaca ccataactga cagatgctcc gtgaaggtat ccctccattt tttctagaac
     9421 agtcgtaccg taatagaatg agtgtgccat agcagaatta tagttattgt taactaaaac
     9481 agaaaccaat ccggtagtaa caatgatatc cgaaacgact tgttcaattg catatgaggg
     9541 acgatcgtcg gcactatctt tcaatgcttg cgtaccataa gtcaagagac gatcaatgca
     9601 catttttccg atttggattc ctaatctatc cgtgtagcct agatcatcgc cacgagcaga
     9661 aaaagtagat tcgacttctt tggataaagc atcgccaata cctgcccata agtatttatc
     9721 aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa
     9781 gaagagtccg tccattacgc cttcttcgtt atagatgaca cataccgcag tgacgggcga
     9841 acagttcgaa ccaatagtgg gaaaggtgaa gactggttta gctagttttt caccaatgac
     9901 ttttacagta tcgcaagcac ggccaccacc gactgcaaac aggacgtctg ctttttggac
     9961 actttcatgc tggatcaact gttcaatatt tccataagtt gaattacccc cgtaccagag
    10021 actggcgaca acctctattt cctgtgtcaa agctactttt attttgtttt ctgcctttga
    10081 aagtgcagtc ttccctccaa tcagcactac ttttgtccca tattctttga cgattccatt
    10141 tgctttttca tacgcatctg ttccaatggt ataatttggt aagaaaacac tttgactcat
    10201 attcatttcc ttctttcttc gttgaaacgg gttaaaattt taaagagtga tcgatctgac
    10261 tgcggctgta gatggagtag aggtgaacct aagacaggat aagtgatgaa taaggtggca
    10321 tgaacacctt gaaatcatca ataagtatcc taaaaacgaa caagtacaaa aacattgaaa
    10381 acgacaataa tgaatttttg ttcctcttag tatttctacc gattcccttt gcttttttgt
    10441 aattatctaa aaattctaaa aattaagatt attatatgaa aacaggtttt atttgtcaat
    10501 tcataattgg ttttttttaa agcaaaagat aatctatatc atgatgaaca aacagagttt
    10561 tttcctccaa ttgatggctt tcctgatctt tcctattttg aataaagtgt ttctgtataa
    10621 tttttttatt ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa
    10681 agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa
    10741 gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag
    10801 ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa
    10861 gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta
    10921 gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta
    10981 gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa
    11041 aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat
    11101 ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc
    11161 cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt
    11221 gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt
    11281 cctcgaccaa attctcaata ataaaaagag gttgtgacag aagtaatcag cttcaagaaa
    11341 taagaaggaa tttacgaaaa ttacttcttg aatttttgtg aaattccaac ttatttccga
    11401 agaagctact tttggaacac cgtgtaccag aggttgtgaa aaagtcgctt agtttcgagt
    11461 agtaagaaaa attttgtgaa aatggctttc tacatttttg cataaatttg gcttaatacc
    11521 gagaaactgg cttttgaaca ccgtttattc agataaggga ctgtgacaag acttttgtca
    11581 cagtttcttt ttgtttattc ttttttatct ttgtatggca ataatagaca atataacgaa
    11641 aattatttaa tggcttttgg aagaccatgg aaaaccttgt acaaagctat t
//
LOCUS       DAPCMT010000089        11564 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPCMT010000089 DAPCMT010000000
VERSION     DAPCMT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.091"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..521
                     /locus_tag="RD743_001941"
     CDS             <1..521
                     /locus_tag="RD743_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563746.1"
                     /translation="FTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKIS
                     LKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICG
                     KTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKL
                     SHETIKKLRIGG"
     gene            524..1354
                     /locus_tag="RD743_001942"
     CDS             524..1354
                     /locus_tag="RD743_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDU0563747.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1370..2221
                     /locus_tag="RD743_001943"
     CDS             1370..2221
                     /locus_tag="RD743_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0563748.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2218..2577
                     /locus_tag="RD743_001944"
     CDS             2218..2577
                     /locus_tag="RD743_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDU0563749.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2592..2753
                     /locus_tag="RD743_001945"
                     /pseudo
     CDS             2592..2753
                     /locus_tag="RD743_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2750..3055
                     /locus_tag="RD743_001946"
     CDS             2750..3055
                     /locus_tag="RD743_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563750.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            3055..3366
                     /locus_tag="RD743_001947"
     CDS             3055..3366
                     /locus_tag="RD743_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDU0563751.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3363..3536
                     /locus_tag="RD743_001948"
     CDS             3363..3536
                     /locus_tag="RD743_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563752.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3533..3949
                     /locus_tag="RD743_001949"
     CDS             3533..3949
                     /locus_tag="RD743_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDU0563753.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3949..4617
                     /locus_tag="RD743_001950"
     CDS             3949..4617
                     /locus_tag="RD743_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDU0563754.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4614..4991
                     /locus_tag="RD743_001951"
     CDS             4614..4991
                     /locus_tag="RD743_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563755.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4988..5287
                     /locus_tag="RD743_001952"
     CDS             4988..5287
                     /locus_tag="RD743_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563756.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5277..5435
                     /locus_tag="RD743_001953"
     CDS             5277..5435
                     /locus_tag="RD743_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563757.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5529..5945
                     /locus_tag="RD743_001954"
     CDS             5529..5945
                     /locus_tag="RD743_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDU0563758.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            6505..6693
                     /locus_tag="RD743_001955"
     CDS             6505..6693
                     /locus_tag="RD743_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563759.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            6683..6943
                     /locus_tag="RD743_001956"
     CDS             6683..6943
                     /locus_tag="RD743_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563760.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            7276..7848
                     /locus_tag="RD743_001957"
     CDS             7276..7848
                     /locus_tag="RD743_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563761.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            7849..8160
                     /locus_tag="RD743_001958"
     CDS             7849..8160
                     /locus_tag="RD743_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDU0563762.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            8151..8411
                     /locus_tag="RD743_001959"
     CDS             8151..8411
                     /locus_tag="RD743_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563763.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            8572..8931
                     /locus_tag="RD743_001960"
     CDS             8572..8931
                     /locus_tag="RD743_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563764.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            8928..10571
                     /locus_tag="RD743_001961"
     CDS             8928..10571
                     /locus_tag="RD743_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDU0563765.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            10606..>11564
                     /locus_tag="RD743_001962"
     CDS             10606..>11564
                     /locus_tag="RD743_001962"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563766.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTV"
BASE COUNT         4200 a         1676 c         2550 g         3138 t
ORIGIN      
        1 gtttcaccgt acagcaacga aacttcatct atgcgctgat aggcgatatt tttatcgata
       61 caggcatgcc aacggacttc tggaaggaat tcttctactt ccgttttgaa ggtgtcacag
      121 ggcgcaaaat aagcctcaaa gatgaatcga atacgactgt gagtgatgct aatgtcttag
      181 caaatatcat cttagatttc atctttgaac atcatattcc tttcaaagaa ggctatgaga
      241 ttttaccagc gaatcaagaa tattacttct acaaatgcat tacaaaaaga gtttgttgca
      301 tctgtggcaa aacaggagct gacatcgatc actttgacaa agcgctagga agacgaaagc
      361 gaaaagaagt tgatcattca gagtacacat ttgcagcact ctgcagaatc catcacacgg
      421 agaagcacaa aataggtgtg atcaatttta aaaataaata tcaaatcaaa ggaatcaagt
      481 taagtcatga aacgattaaa aagttaagga taggaggata aatttggctg agataagttg
      541 gatcaaactt aaaactacta tgtttgacga tgaaaaaata cggttaatcc aagctgttcc
      601 tgagtcggat gccatcatcg ttatatggat tcgattacta gttttagcag gaaagactaa
      661 cgacgatggt ctgatatata tccagaggaa catgccttat accgaagaaa tgcttgctac
      721 attgtttggc aaaaacgtaa atacggttcg cttagcgcta actacattgg caaatttcaa
      781 catgattgat ctaagcagtg atggactaat tgccatcagt aattgggaaa aacatcaaaa
      841 tatcgagggt atggataaag taaggctaaa aaatgctgaa agaaaccgta aatacaggga
      901 aagaaagaga caggaacgtc tcaaattgga aaatgacgtt agcgtgacgt cacgtgacgg
      961 tacagataaa gatatagaag aagataaaga tatagataaa gaagaaaaga aaggtaagta
     1021 ttctgacgaa cacttacgcc ttgctaaaaa aatgcaaagg aatttaactg aagattttcc
     1081 aaaagaaatg aacaaagtag atatcgaaaa atgggcagac acaatcaggt tgatggaaga
     1141 aagagataaa gcatctatag aagcgattga gtatgtgatc aattggctac ctacaaatga
     1201 attttggttt ggaaatatta gaagtgctaa gaaattgaga gaaaaatttg agaagctcaa
     1261 attcgaaatc aaagcagaca agaagaatca taaaaagcaa agtcaaaaac tacagtacag
     1321 caatcctagt gaatatgacg acttgccaat ttaaaaggag atgcatcaca tggaaagcct
     1381 agcaaatgct atggagaagc taataagaag agtattagtg caaagcggaa aatgtccaga
     1441 atgtagcgaa cctttgtata gttggcgagc taaaaataag gatggttcag aacgttgtaa
     1501 accaacatgt atgagttgtg gttataaagc gttacgtgtg aaagaggata tacagaccga
     1561 acggatatat aacgacagct taaaagcacg agcattgagt ttttttcaaa atggttcggt
     1621 attaacagat aaaactttgt ttaaatgcaa aatggagaat tatcacgtag tggaccaaga
     1681 aacgaaaatt gctttagaaa gagctaaaag ctatgtaaat gatgtcctac tgaaccatcc
     1741 tgcacatttc attctatcag ggaaatcagg aagcggaaaa agccacttat caatggcgac
     1801 agcttgggaa atacttgagc gctcaaatta tgacaagaaa atacttttta taagctatca
     1861 agagttatta gagcaaataa agttttctta taacaatgct gaactgagaa aagaaattga
     1921 aggatcgctt atagccgata tcaaaacaac tgatttggtg gtttttgacg atattggagc
     1981 tgaattaggt agcggggtat caaatagtag gcaatttaca aacaacacgt taaacacgct
     2041 cttagaagcc agacagaaca aggcaacgat catcacaaca aacttatctg gtcctgaact
     2101 aagagaagcc tacggtgaaa gaattgtttc taggatattt aagaattcag aaggttatgc
     2161 gctgaaattc caacaaacag cagacaagcg cataaaacca gtgaaaggta gtatcgcatg
     2221 aataaatacc gtaataaaaa aactgttcat cgaggtatca agtttgattc tatcgcagaa
     2281 gcagagtatt acgatctagc cttgtggcaa gctgaagcga acggctggaa agtaaaactt
     2341 caggaaagat ttgagctgat gccgaaattt gaactagacg gaaagaagta tcgcaagatc
     2401 gagtatattc ccgacttcac attttataaa aacggcaaac tagtcaaagt cgtagatgtt
     2461 aaagggatgc agacaaaaga tttcaaaatc aaagcgaaac tcttctgcag tcaatatcga
     2521 gtaccactga tcttagcgaa gaaatatcgg aatacgttca aggaagaacg gttttaacga
     2581 ggtgatccat catgacaaca gaagaagtga ttcaaatgcg tattcgaagc cttcagcgtg
     2641 agattgacga tctgtaacgg acaaaggcag tgatggtcaa tgaaacggcg aggaaggcaa
     2701 tcgatttgca catagaaaat ttaagaagga aaatccatcg attggaggaa tgagcgtgga
     2761 taagaaagca gcaatgaaac gaatcatcga actgacacat tctgagaatt ggcaagaaga
     2821 caaagaaata gttgcagaag tccaaaagct cggtaaatca atgtgggctg aaaagcctaa
     2881 acggaaaacg ccgagaaaaa ttgcaatctg gcatggtgat cgaattctag taacaggtac
     2941 tgctgaacag ttatctgaaa ttactggatt aagcaaaaac attatctggg atagagctag
     3001 gagcttatgg attgattcaa aaggacgaca gtttaggtat gtggaggaga aataatgaac
     3061 gaactaatca caaaagtaga gcagtgggct aaagacaagg gactggatca agcggatcca
     3121 aaagcacaat ttttgaaagt agctgaggaa ttcggagaaa ttgcatcggc aatggcaaga
     3181 agtaatgatg agctatttaa agatagcgta ggagacgtta tcgtcacgct gattattctt
     3241 tccatgcaaa aaggggcaaa catacaagag tgtttagaaa tggcgtataa cgaaatcaaa
     3301 ggacgcacag gaaaaatggt agatggtgta ttcgtgaagt cgagtgattt ggaggacagc
     3361 gaatgatgat gccaaaagaa atctattcag atgcattaag aggtgctaga aatcaattga
     3421 aaatggcaaa aagagcatac gaaattcggc ccacaatcga aaatgaacgc agagtaaaag
     3481 caattcgccg tagatgttca atttacggcg agttacagaa ggaggacagc aaatgatacc
     3541 gagatttcga gcttgggata aacgaaagaa cgtaatgaga gatgtagccg tcttgcattt
     3601 tactaaaaac ggcaaaacaa actttattga atattggata aatcctaccg aattgaaatc
     3661 atatcacgtg cgaaacatcg agctcatgca atcaacaggg ctgaaagata agaatggcat
     3721 tgagattttc gagtgggata ttgtatcagt cagcgtgcga aatggattcg attatttaga
     3781 taataaagtt tgtgttgtca aaaattcaat aggacattcg ggattagtgt gtgccactgt
     3841 tgatgaagat ttagagtatc aaatttttaa cacagagctg tttgaagaat acacgtatga
     3901 agtcatcgga aatatttggg agaatagcga gttattggag gaacagtgat gaataaacag
     3961 gaattgattg atgaattagc taaatatgta aagcgttatg agaacgttat ggatgagcat
     4021 ggccaaggaa ggtacggcgc ttatgaagta tctttaaagt tggtgaaaag actaaatgaa
     4081 tcaaaaatta cggacgaaca agcttggaat aaggtagctg aggcttatcc tgaatcggca
     4141 caaagcttga gaaacacttt agataatgct gtatttggta agactggtga accgcagaag
     4201 cacgttatgc cgaagtttgt ggcggagtgg atagaatacg ctaaaaagaa aggtgatagt
     4261 ctagctattt cattcaagcc gtggaacctc tatggtgttg agtatagcaa ggctgataga
     4321 tggattggag ataatcaaga aacgtttgct cgtgcttggc tagacggcta cgaggtcgag
     4381 aaagagccgt tgtattatgt cccgctacca tatgaagttt gggatgaaga ggcagcggag
     4441 cttaaaacag agtatttgta tctacattat gagatcacaa gtgatgaaac tagaatattt
     4501 cctacaaaag aacctagaaa aggttttgtc gcaaaattag atgaacttac gattaaatca
     4561 gcagatgaaa actactggcc gtttgctgta ccagtggaag aggtggtaga aggatgaaaa
     4621 aattgcacca actcatatct gagaaagaaa gcgaactcca aaatttagaa gatagtttgg
     4681 gattggggtt tccaattgtt gagcaagtta aaatggttca aatcagtcat ctacaacttg
     4741 aattggaaga tttaagacaa atagaagatc cgtatcaact caacgataac cagcaaatcg
     4801 tgttggagtg gttgaaacta actgcgtcaa caggaaagcc aatgcaagtt gtattttgga
     4861 tgatgaataa tgcagcatgg gggcatttag acgaattgag ggatcctttg atggaattaa
     4921 ctgacaaaca gcaattcgaa gtcctaacag cattcgctca atggggatta gaacaggagg
     4981 aaaaagaatg aaactaaaag acggatttta cgctagtagc cacggtatcg gcggtttaat
     5041 gctagacatg ccgacaaaga accctaaaac acgtaagaag ccaaaagtca aagtcggtga
     5101 catggttcgc tgtgaagcag aaggattcat ctatccattt cgtggatatg tagaacacgt
     5161 ctataatcac tcagcgatca ttcgcattga aaacacgatg gaatgcgaca agttattagc
     5221 gaaaagcaaa gataatttag cagtggtgag attggtggat atggaggtta taaacaatga
     5281 aatttaaaat ctttgaagag gacactcgct ataaattaga aaaaaaatta aatgaatttg
     5341 caaagaataa tgaaattcag catatatctt taacggcttc taagtcaggt tatacaactt
     5401 actatgcagc tgttgtgagc tacgtaattc aataaaagga actcggcaaa taaaaaagcc
     5461 ggatcactcc gactgatgta ataaatccga caagtttatt atatcacata aaggagcggt
     5521 ttgacttgat gcaattgtta cgagaggtag atttcaaaca gacaagatgt aatgcgagag
     5581 atgtgctgaa gaactttcgg cgcttggagc ggatggcagg tcgctctttg atagatatta
     5641 agtcgccgat tattacggat atgccgaagg caccgaagca cggcaataag gcagaagacg
     5701 cgatcattca gatgatggat atagaagcgg agagagacgc gattttagcg gctttgatgg
     5761 cgcttagtct gattagccgt cagatactct actacagctt ctgtgacgta aacaagcact
     5821 ctaattatga aatagggcaa ttgatacgag gatacggaga gaagaatgta gagaagctga
     5881 aatccatcgc attgatcgaa tttgctgaag catacaaaaa aggcgtgtta gttcagtatc
     5941 gttaattttg tagggttttt gtagggatag tgtagggttt ttgagcggtt taacgtgata
     6001 ttatgatagt gtcgaaagat tagtgatagg tctaagacaa aataataata aaaggaacat
     6061 cgttttatta ttgtttcaca attaagcttc gatagacagc agcggaaata ttaagaataa
     6121 ggatgtgaat tttaactcct tctaaattgt tcttattatc tatcatcagt tgctgtctat
     6181 tgtcattatg tcactgtggt ggaaaggtgt atcgctcatc taaaatttag gtgcaaactg
     6241 caaggttcga ttccttgcca gtgactttgg taaccgaggc atcggcggtt taaaaatata
     6301 ggggtgcgca atttcgtacg cgttttgtgc atcttgtagg ttgctattac ataattggtt
     6361 gggttagatt gagttttggg attcggtaca aatgaatcgt caaatgactc aagcacagga
     6421 tcggaaacgt ccctgcctgt gcattacata ttagatcact ctttgagtgg tctttttatt
     6481 ttaaaaaaga gaggattttg aaaaatggaa ttgattgtat ttactaataa cggacaaaca
     6541 tatcattttt atgaagtgga gaattttaag ccaacaacaa caggatttag ttttatttat
     6601 actgggaagg ctacgggagt tactcgttca gctacgttta acaatacatc aacagctggt
     6661 tatgcgcttg cggaggtaca aaatgaatca tgagaagttt atcgaaaaat gcaaggctat
     6721 tgtacgtgaa agaattgaaa atgagattgc tgacctaagt ggagcagtgc ctgagtttag
     6781 tattttcata gtctggtcat gcaaaacact gcaaaatagc aaagcattag tcagcgctag
     6841 cttaaaagga gcaccgtatt ttgaaattac gttgaacggg gacaaaggtg aaatctatgt
     6901 agatacttat ctcaaaaaat caaatgaatg tatcaaagtc tagcaggtgc taggcttttt
     6961 ctttacataa aggaggctgc ataatgagaa actactggta tgtatcacta actaatgaat
     7021 atcctcgaac cattggtgat tgttcagtgc gtgttgtgcg ttctgtacaa atcaaaggga
     7081 agtactctat tgtcgaaatg ctaagagaag ctacaccaaa cgaagtggat aaatgcaagc
     7141 tgatatattg cggtcatggc tattggaaag acgagtatat ccaatacaac attgagaggt
     7201 acatcaaatg aatgataact atgattacat caagttgtta gcaagtaatt attagatgtt
     7261 tttaggagga aaactatgaa attaaaagat tatatcagag aagggtataa cgttgtaact
     7321 gcacccaatc tagcttacaa aattcaagaa gattatccca atgctttagt gtttactgat
     7381 agagctttag atgccattcc tatagggaag ttattagtag atcgttttta tagcaataat
     7441 ccagctgttc ttagtgctaa gccacttcaa aacgcttatg ctattgaacg ctttacttgc
     7501 gagtttactg gatatgagac ggtagtacca atgacaggtg agacaggaag aaaagtaatt
     7561 caacagatca aaaagaggat tgctgaattg acgttagatg atggaagtaa tacgaacctt
     7621 ctagaaatca aactaagaga tactgactca gtaccagaag tttattataa aggtcaacga
     7681 atggatgaga tgccgatagg tcttgtcgat attacttatc attgggatac ggaagaatat
     7741 gaatgccaag taccaaaagg agctaacgac atcactattg aatattatga ttcacataat
     7801 aacaagtact tagatagaaa aatcattggg cacaagagag atatgtaaat gattgatgtt
     7861 tcaagtaaac aagcaagagc taggttctat ggatcaactg agtggaggaa acttagaagt
     7921 caatgcttag agcgagatca ttatgagtgc caatggtgta aagcagaggg tagactaaca
     7981 actcaacatg attcaattct tgaagtggat catataaagg agctggagta tcatccggat
     8041 ttagctctag atataaacaa cctaagaacc ttatgcaaag attgccataa caaaaggcac
     8101 aaccgtatga aatatcgtgg ccaacaaaag aaaagaaagt gggatgatga atggtggtaa
     8161 gaaaaacagc aaacagagat gatgtcaaac gagcagaagc aatcgctatg gttgataagg
     8221 ctattgataa gttggttcta tttaagagtc agctcattga aggaacaaca actgtagagg
     8281 atatatctat caacaataga ctaataggta acgctaatga agtaacaggg acatttaata
     8341 ttaatgtaga cattgattat accttgagtc catttgcaca tattgtagaa agcaacgagc
     8401 aagacggctg ataaatcagt ataaagcgaa gatatataga gtgaaatacc cccggtcaaa
     8461 agattttgag aagaaataaa atttttggga accggtggat ggggtcaact ctgcaaataa
     8521 atgatgctat tttcacacga ccccctcccc acgtagaaag gaagtgagtt gatggctgat
     8581 ctgcaagaga gaaataggct tgtttctgct gaagaaaggc gtctaaataa attgtttaag
     8641 gatattgata aggataaaaa gaaggtcgtt tctggtttga ttacgcaagc ggcacgctta
     8701 aaaattcttc ttgatgaaat gtggattgat atttccgaaa aaggtgatta tgagcttttc
     8761 tctcagtcag aaaatcaaaa accttacgag cgggaacgac cagttgctaa acagtataat
     8821 tcgagagacc aagcatatca acgtgtaatt aagcaactta ctgactattt gccagaagaa
     8881 aagcgcgaac ctacaaaaaa tgcagcgcta gatggtagtg atttgctgtg acaacattaa
     8941 agccattcta ttttgatgaa tacgtagatt tatacgaaaa aggggtaatc ccttttaata
     9001 aagaaagaat tcagttaatt acttatttaa aaaacgaagt gctagttcgg gatgatatct
     9061 attttgatga agaaatgatt caaaagttta tcaagtacac tgaaaaaaat ttttttcctt
     9121 tagctaaata tcaaaaattt atcacacctt tcattttctg ctatcaaaaa gaggacgacg
     9181 aggtgttttt tgatgaaatt ctaaatagta ttgcgcgagg cggcgggaaa aatgggttca
     9241 tgtcagcaag agactcattt tttattagcc ctctctacgg tgtcagaaat tatgatgtaa
     9301 cgattacagc gaattctgag aagcagggaa aggtatcttt taaagaagtt tatgaaatgg
     9361 tacaaatgcg caaactggaa aaacaatttt atttgactaa gatggctata gtcaacaatg
     9421 taactaattc gatttttagt tataggacaa acaatcctaa gactatggat agtgcaagag
     9481 atggctgctt agaatttgat gaaatccatc agtttgaatc ttcagaccta gttgatattc
     9541 agcgtagtgg gctagggaaa ataaaacatc ctcgaacttt ttataatggt acaaatggtc
     9601 atgttagaga aggtttttat gacaaaatgt tggaacgagc acaaaagatt tttagtggag
     9661 agaacaagaa tgatcgcttg tttcccttta tttgtaaact ggataacatt gatgaaatgg
     9721 ataatcctaa aatgtggtca aaggctaatc caatgttcga agaagattca gcatattcaa
     9781 aacgtctgtt ttctacagtg atgaaagaat ataaaaaact agaagatgag ccatcaggta
     9841 ggcgtgaatt tgttgttaaa cgaatgaatt ttacagaagg tgatgcagaa tcagatatta
     9901 cgactcatga aaaattaatg gctactaaac aagaattgcc aaatttgaat ggtcgttctt
     9961 gtgtggctgg atttgattat gcaagtattc gtgattttgc aacagtgggt ttgcttttta
    10021 aaattgatga taaatttatc tggcagcaac atagttttgc taggaaaaag tttctagata
    10081 cattcaaact gaaagcgcct attaaggatt gggagaaaaa aggtttagtg acaatcgttg
    10141 atgagccatc aattgatcct cgtcatttaa ttgattggtt aaatgaaaaa agaaaaatcc
    10201 atcaaataga aatcgtttgt gccgacggat ttcgtatgga tttgctgaaa cctcttctag
    10261 agaaagaatc atataatttt gagtttatca gaaatataag aggtgtgcag tcaaaagttg
    10321 ctcctattat tgaagatggt tttgcaaatg aacggtttat ttttggtgat gatcctatga
    10381 tgcgttggta tacaaataat tcgtttatca aagaagacaa attagggaat aaaacatttt
    10441 tgaaaaaaga gccagtaagg cgtaaaacag atgggtttca tgcttttttg gcagctttat
    10501 ataaacgaga agaaataact gatatggatt atgaggaagc atttgatatg ttggatgaga
    10561 ttgaatttta aggtatactc cttattgaaa ggggtagtta tagaagtgaa aaagagatat
    10621 tttctatttg ttacaatatt tataataata gcaatttgtg ttttgacagt atttttacca
    10681 caaattactg agtatttaaa ttatcggata tttaccacaa aagtagatga tagcgttact
    10741 agtttaagta ggctagaatt tataaaaata tgtgcatctg tgtttggtcc tattttgact
    10801 gtaatggtat ttgtgaatac tttaaatatg cagaaaaaaa cagagcaaaa gcaaaatgag
    10861 cttgaagtaa agcaagctaa ggctgagaac gagagagcaa aaaaagagct gttaacacag
    10921 atagatcgtg agttctattt tttattaaat atgtttattg aaatacaaaa agatcaaaat
    10981 gccaaacaag ctgttattgc tttacagaag ttggcaattt ttaacgaaaa gggttttaat
    11041 caagactatg catttacttc taaagattta tcagatagtg aaattgggag tacaattgaa
    11101 caatcaggac cgctgaaggg cacagttgaa tatataaaat ttgaaacatt tgatgtaagc
    11161 aagtcgatgg ggaattatca aacaaaaaca aaagacgttg ctacaagttt acacgatcag
    11221 aaagtagcta tattgaatga acaatatgaa aagatgcaaa tatatcttgg acggtacttc
    11281 aaaatgtttc accgaatagt taaaacatta aatgagtatt atgacgatta taatgatttt
    11341 gatgtgaaga ggtatacaaa atatataggg acattaagaa cacaaatttc tccagctgaa
    11401 ttccaagtga ttttattcaa ttctctatat ataaaacgag gttttggctt aggaattcaa
    11461 cttattggaa gtggtttttt tggagatgat tttgattttg aaactaatca acattttgaa
    11521 acaagtatca atgaacagtg gtttctatca ctatcaactg ttga
//
LOCUS       DAPCMT010000090        11424 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPCMT010000090 DAPCMT010000000
VERSION     DAPCMT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.092"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(26..571)
                     /locus_tag="RD743_001963"
     CDS             complement(26..571)
                     /locus_tag="RD743_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDU0563767.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(583..1275)
                     /locus_tag="RD743_001964"
     CDS             complement(583..1275)
                     /locus_tag="RD743_001964"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDU0563768.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1294..1578)
                     /locus_tag="RD743_001965"
     CDS             complement(1294..1578)
                     /locus_tag="RD743_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563769.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1591..2154)
                     /locus_tag="RD743_001966"
     CDS             complement(1591..2154)
                     /locus_tag="RD743_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDU0563770.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2382..3044
                     /locus_tag="RD743_001967"
     CDS             2382..3044
                     /locus_tag="RD743_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDU0563771.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3168..4364
                     /locus_tag="RD743_001968"
     CDS             3168..4364
                     /locus_tag="RD743_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDU0563772.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4465..5841)
                     /locus_tag="RD743_001969"
     CDS             complement(4465..5841)
                     /locus_tag="RD743_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0563773.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5878..6744)
                     /locus_tag="RD743_001970"
     CDS             complement(5878..6744)
                     /locus_tag="RD743_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDU0563774.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6833..7858)
                     /locus_tag="RD743_001971"
     CDS             complement(6833..7858)
                     /locus_tag="RD743_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDU0563775.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7876..8550)
                     /locus_tag="RD743_001972"
     CDS             complement(7876..8550)
                     /locus_tag="RD743_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0563776.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8734..9627)
                     /locus_tag="RD743_001973"
     CDS             complement(8734..9627)
                     /locus_tag="RD743_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDU0563777.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9701..11398)
                     /locus_tag="RD743_001974"
     CDS             complement(9701..11398)
                     /locus_tag="RD743_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDU0563778.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3386 a         2514 c         1962 g         3562 t
ORIGIN      
        1 tattttatca aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat
       61 gtctcaatgc ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat
      121 ggagtttgta tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac
      181 atacgccggt caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg
      241 ttccactaaa agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat
      301 agtcttggta taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat
      361 tatgcggagg aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa
      421 tggcaaaaac gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt
      481 tttcaattac ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat
      541 atgtgtagtc aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac
      601 aaattcgatc accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct
      661 ttgtgtcgcc gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt
      721 gaactgacga gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg
      781 gaaattcgtt aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac
      841 gatcaatcct tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg
      901 caaatatgtg cttgcttcat aatatagagg cattcctggt aattgtcctg gtttgtagcc
      961 aaatcctgtt gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac
     1021 ttgtaagcct tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta
     1081 ttgttggatc aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac
     1141 gacgatcact tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca
     1201 tgataatggt tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat
     1261 aataccgatt ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac
     1321 cacacataac attaggataa caataataag agatttcatc aaaaaagagt tccatttggt
     1381 cgtcttttcc cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt
     1441 acgataaaag ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg
     1501 tttttttaat gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat
     1561 aagcggtcct ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta
     1621 ttgaatcgca taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc
     1681 ctcttcaaga gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt
     1741 ctcgaccttc tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg
     1801 tgaaagatac atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc
     1861 taattctcta gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc
     1921 tggaatctcc aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg
     1981 atcttgttcg tcaaaagcaa tgatcccgac accaccagga tgaaatacca gttctctctt
     2041 cgaagttcct ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct
     2101 atagatttct ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc
     2161 tcctgctttc tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact
     2221 ccttattagt ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt
     2281 agtccgatac tctttctagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa
     2341 agaattcaat actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc
     2401 cgtggcttct agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg
     2461 ttctaggact tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca
     2521 ggaaaaaggg aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg
     2581 aaaaaagcgg tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa
     2641 aaaatgagat caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg
     2701 atctccgcca tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac
     2761 caaaccgact gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt
     2821 tgacaaataa gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa
     2881 aactagaaga aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc
     2941 aaaaattcgt tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga
     3001 gcttaaagcg tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa
     3061 agaagactcg gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag
     3121 acattcatgt aagaatcgag aacagattca acaaggagga attttttatg gatcataaac
     3181 cgactgaaac aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc
     3241 cgttttcaac tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc
     3301 aagagcaaca gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag
     3361 ctaaacagct agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat
     3421 cggctgccca agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac
     3481 aagatattgg tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca
     3541 atcctgacga actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca
     3601 aacaatcgat ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga
     3661 tttcagtgaa attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac
     3721 agctttacca gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag
     3781 aattgaaaat ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag
     3841 aaactggcga tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc
     3901 tggataaacg tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac
     3961 aaatccgttt gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg
     4021 ctacagccat tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa
     4081 aagatgcagt aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa
     4141 attcagaaat gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg
     4201 tggatatcga gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat
     4261 tgcgtatcca aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca
     4321 tggaagaaga tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt
     4381 tactaaaaca aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct
     4441 ttcttctaac ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata
     4501 gccaaaataa atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt
     4561 ttgcagacga agcatcaata caatacaagt caagatggag acgattggca atagcggaac
     4621 gaatggcact ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa
     4681 gcctagacta acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac
     4741 attcaatggg aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt
     4801 tttcggtgta cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt
     4861 gctgacagca aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag
     4921 cgttaaaatt gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat
     4981 tgcaaaagcc actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat
     5041 cagcgtcacg atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg
     5101 cacatctttt tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa
     5161 gaaagcaaaa aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa
     5221 tggctgccag ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat
     5281 accaaatttg acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga
     5341 cacgatgtaa gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt
     5401 ttccggattg tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa
     5461 accttgcata tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc
     5521 gatcaacaac caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc
     5581 atatggccca ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa
     5641 aatgacaacg attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt
     5701 ggccgcaacc cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat
     5761 gatcaaatca gacgttttta gttctttttt catctcactc gtatgattga gattgatctc
     5821 tttcttacga aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta
     5881 aactgttttc atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat
     5941 aacataaatg agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt
     6001 agagataatg atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac
     6061 tgttagaagc atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca
     6121 cacaaactga tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc
     6181 gatactttga ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt
     6241 atatacacca taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag
     6301 cattttatta tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc
     6361 gcttacaaga agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc
     6421 atggaagaaa acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg
     6481 tagccctaca gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact
     6541 ccaagctaag ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac
     6601 aacgaaaaag acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg
     6661 attggcatcg ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg
     6721 gactaatgct attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt
     6781 atcagcgaaa acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt
     6841 tttcattata aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag
     6901 ttgattcagc cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc
     6961 ctaatcctgt tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa
     7021 agatccgttt caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga
     7081 ctccttgcgc ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga
     7141 tggcattact aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag
     7201 tatgatcctc accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac
     7261 tgcgtatcac aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg
     7321 agaaactgtc gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata
     7381 attcattttc tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta
     7441 gcaatttaga agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt
     7501 tttggtcttg ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct
     7561 gattgatata ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt
     7621 gcaaagggga agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt
     7681 aaccacccag cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg
     7741 tgctcaaatc aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa
     7801 aaaacagcca tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct
     7861 taaccgctca tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc
     7921 ttggatacct gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt
     7981 gtcatctaca aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat
     8041 ctttccattg tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat
     8101 gatctctccg ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct
     8161 catcatttca ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt
     8221 tgcaatcagt acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat
     8281 agccatgatc atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga
     8341 tacttcacgt attttttgac accaataata gccatccaaa acgggaagat taatatctag
     8401 taatacaagc tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt
     8461 tccaaaggtt tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc
     8521 ttcgtcttcg actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac
     8581 tacaatatca aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc
     8641 aaacgttaaa aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt
     8701 aaatttatta tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt
     8761 ttgtctttta aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc
     8821 tagaaggagt ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga
     8881 gacttcttct gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt
     8941 gtaaggtgta caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt
     9001 tgtttttgtt ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata
     9061 cacttcaaga tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt
     9121 ctcagcaata ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa
     9181 agaggtgcct tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa
     9241 taaaggcaag ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta
     9301 atattctttt cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg
     9361 tcccattcct gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc
     9421 taattgttgc tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat
     9481 ccttctttga tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc
     9541 agctccggca agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg
     9601 aggtctatta cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc
     9661 aacctttata aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt
     9721 tagatttacg gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg
     9781 ccaagagccc gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt
     9841 ctggccctgg attttctact cctacgatct gaatttcttc attatagctt ccataagaga
     9901 tgataaattc agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg
     9961 tctctttcat ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat
    10021 ctgcgacaaa ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt
    10081 cataaacagc taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat
    10141 tccacaattc gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa
    10201 actttcttcc acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt
    10261 catcatttcg tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt
    10321 ctgtggaaac cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc
    10381 tacctaaagt atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc
    10441 cgttgcttac tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc
    10501 gcacttctat ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt
    10561 aatttttgat aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg
    10621 ctgtgatcgt gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt
    10681 gttctccagc ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct
    10741 gtttatctgc ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt
    10801 ctttccatgt agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca
    10861 taacaaccat tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa
    10921 gatagacttt atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg
    10981 cttctccagc ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc
    11041 aactttctgt gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt
    11101 ctcgttccgg tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa
    11161 acaaatcaaa atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc
    11221 tattcggtcg ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt
    11281 cacttggttt ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta
    11341 ttcccgcagt aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt
    11401 ttttctcccc gttctttttt tatt
//
LOCUS       DAPCMT010000091        11177 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPCMT010000091 DAPCMT010000000
VERSION     DAPCMT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.093"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(241..1089)
                     /locus_tag="RD743_001975"
     CDS             complement(241..1089)
                     /locus_tag="RD743_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDU0563779.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1088..2155
                     /gene="gnd"
                     /locus_tag="RD743_001976"
     CDS             1088..2155
                     /gene="gnd"
                     /locus_tag="RD743_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDU0563780.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="RD743_001977"
     CDS             2157..3158
                     /locus_tag="RD743_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDU0563781.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="RD743_001978"
     CDS             3155..3439
                     /locus_tag="RD743_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDU0563782.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="RD743_001979"
     CDS             3472..4269
                     /locus_tag="RD743_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDU0563783.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="RD743_001980"
     CDS             4283..5116
                     /locus_tag="RD743_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0563784.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="RD743_001981"
     CDS             5134..5991
                     /locus_tag="RD743_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0563785.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="RD743_001982"
     CDS             6013..6522
                     /locus_tag="RD743_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0563786.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="RD743_001983"
     CDS             6559..6960
                     /locus_tag="RD743_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563787.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="RD743_001984"
     CDS             7072..7425
                     /locus_tag="RD743_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563788.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="RD743_001985"
     CDS             7459..7902
                     /locus_tag="RD743_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDU0563789.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="RD743_001986"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="RD743_001986"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDU0563790.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="RD743_001987"
     CDS             8997..9710
                     /locus_tag="RD743_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDU0563791.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="RD743_001988"
     CDS             complement(9917..10066)
                     /locus_tag="RD743_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563792.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="RD743_001989"
     CDS             10462..10662
                     /locus_tag="RD743_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0563793.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3756 a         1745 c         2407 g         3269 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgtggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg gttttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaaag
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa attcattggt cgttgaatgg
    10741 gctgaatagt agtgtataaa acaattttgt ggagcgtgaa gcttttatgg tagaggaaaa
    10801 tacgaataaa gtgatgttga taaaaagatt cctatcttga atatgaatcg agatgtcttg
    10861 aaaatgaatg agtttgacca atcattatcc tatcgaaatt tacagtttcg ttcgattggt
    10921 ctgtcaatta atgaaaaagt aaaaaagaat atgttctagg aagagggcgg cacatctaaa
    10981 tttttaagta acggacagtt caaatatcaa gcattgaaag aggctatcct actgtttgaa
    11041 ggaatacgaa tatataaaaa cgacaaagag gaaaaagtag ttcattaaat cccttcaagt
    11101 gatgaaagaa tgagatctaa taaaacctga tctttcatct gtttttttaa taacgtaata
    11161 atttttttat tttttgt
//
LOCUS       DAPCMT010000092        10929 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPCMT010000092 DAPCMT010000000
VERSION     DAPCMT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.094"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..288)
                     /locus_tag="RD743_001990"
     CDS             complement(<1..288)
                     /locus_tag="RD743_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0563794.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="RD743_001991"
     CDS             430..1548
                     /locus_tag="RD743_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDU0563795.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="RD743_001992"
     CDS             1676..3052
                     /locus_tag="RD743_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDU0563796.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="RD743_001993"
     CDS             3453..5033
                     /locus_tag="RD743_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDU0563797.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="RD743_001994"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="RD743_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5659..5979)
                     /locus_tag="RD743_001995"
     CDS             complement(5659..5979)
                     /locus_tag="RD743_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDU0563798.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6127..8361)
                     /locus_tag="RD743_001996"
     CDS             complement(6127..8361)
                     /locus_tag="RD743_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0563799.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8840..9106
                     /locus_tag="RD743_001997"
     CDS             8840..9106
                     /locus_tag="RD743_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDU0563800.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9106..10833
                     /gene="ptsP"
                     /locus_tag="RD743_001998"
     CDS             9106..10833
                     /gene="ptsP"
                     /locus_tag="RD743_001998"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDU0563801.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2216 g         3322 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5641 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5701 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5761 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5821 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5881 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5941 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     6001 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     6061 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     6121 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     6181 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     6241 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     6301 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     6361 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6421 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6481 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6541 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6601 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6661 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6721 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6781 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6841 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6901 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6961 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     7021 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     7081 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     7141 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     7201 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     7261 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     7321 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     7381 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7441 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7501 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7561 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7621 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7681 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7741 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7801 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7861 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7921 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7981 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     8041 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     8101 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     8161 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     8221 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     8281 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     8341 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     8401 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8461 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8521 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8581 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8641 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8701 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8761 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8821 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8881 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8941 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     9001 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     9061 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     9121 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     9181 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     9241 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     9301 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     9361 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9421 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9481 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9541 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9601 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9661 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9721 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9781 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9841 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9901 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9961 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
    10021 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
    10081 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
    10141 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
    10201 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
    10261 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
    10321 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
    10381 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10441 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10501 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10561 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10621 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10681 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10741 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10801 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10861 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10921 tagacaaaa
//
LOCUS       DAPCMT010000093        10922 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPCMT010000093 DAPCMT010000000
VERSION     DAPCMT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.095"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..132)
                     /locus_tag="RD743_001999"
     CDS             complement(<1..132)
                     /locus_tag="RD743_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDU0563802.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="RD743_002000"
     CDS             542..841
                     /locus_tag="RD743_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0563803.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="RD743_002001"
     CDS             914..2275
                     /locus_tag="RD743_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDU0563804.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="RD743_002002"
     CDS             2297..2557
                     /locus_tag="RD743_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563805.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="RD743_002003"
     CDS             2571..3323
                     /locus_tag="RD743_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDU0563806.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="RD743_002004"
     CDS             complement(3417..4022)
                     /locus_tag="RD743_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0563807.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(4075..5049)
                     /locus_tag="RD743_002005"
     CDS             complement(4075..5049)
                     /locus_tag="RD743_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDU0563808.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5427..6146)
                     /locus_tag="RD743_002006"
     CDS             complement(5427..6146)
                     /locus_tag="RD743_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0563809.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(6273..7544)
                     /gene="celB"
                     /locus_tag="RD743_002007"
     CDS             complement(6273..7544)
                     /gene="celB"
                     /locus_tag="RD743_002007"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDU0563810.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7697..9094
                     /locus_tag="RD743_002008"
     CDS             7697..9094
                     /locus_tag="RD743_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0563811.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9175..9615
                     /locus_tag="RD743_002009"
     CDS             9175..9615
                     /locus_tag="RD743_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDU0563812.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9763..10089
                     /locus_tag="RD743_002010"
     CDS             9763..10089
                     /locus_tag="RD743_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDU0563813.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10090..10839
                     /locus_tag="RD743_002011"
     CDS             10090..10839
                     /locus_tag="RD743_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563814.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3485 a         1927 c         2249 g         3261 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttagaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc
     4081 acgtttttta tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg
     4141 acgttcttct aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc
     4201 ttcaaggaat ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa
     4261 gtactcagat tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt
     4321 gattacttga atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg
     4381 gatatttcca aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct
     4441 tgcaccattt cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc
     4501 tttgaatcct aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac
     4561 aaagacaggt ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg
     4621 aatgccagct gctacacctt gattgttttc gtcagagatc aattgataat actgattatc
     4681 gaaatcttca aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac
     4741 gtcatatttt tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa
     4801 agaagcacga gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa
     4861 gaaagtcata tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc
     4921 gatgtcatct tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa
     4981 gcggtagatt tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg
     5041 tgtatccatg ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat
     5101 agtatagagg gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa
     5161 tcaagaatgg aaaagaggag ggacggatca ataaaataaa aagacttact actagctgta
     5221 tcgatttcaa aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa
     5281 gtctttttat gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg
     5341 ttgatttcac gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt
     5401 aaacggcttt ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc
     5461 cgaacatcaa atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag
     5521 acgacttgct gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca
     5581 tattcatcac ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat
     5641 tcttgatgaa gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca
     5701 agatggactg gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac
     5761 cggtgaatat tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga
     5821 ttagggaatt caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc
     5881 atttggagac tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga
     5941 tgattcaaga ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag
     6001 atattgatgg cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact
     6061 tgattaggca aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata
     6121 tttgcgattt cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt
     6181 atgcttccga caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg
     6241 acaagttttt gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta
     6301 tctagtactt tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc
     6361 agcataccac caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc
     6421 catggaacag agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg
     6481 aacgtcgtta cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg
     6541 atcggtagac cgaagatcgc tggttcattt acattgaaaa taccaggtgc taaagctaat
     6601 cgtccaacat cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct
     6661 aaagtcattc cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg
     6721 atgtgtggta attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc
     6781 caaatcgggt ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag
     6841 aattggacaa agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat
     6901 ggtttttgga tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc
     6961 acagcattga ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat
     7021 gattttgtta ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg
     7081 attctgcagt agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt
     7141 gcgccaagac gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt
     7201 gcaaaaggcg taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca
     7261 acgtcataag attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg
     7321 ctcattgttg cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca
     7381 ctgaaaaaag gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag
     7441 gaaagcatga aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct
     7501 aagggcatga ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa
     7561 tttatttgca atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt
     7621 ttcataaaaa gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa
     7681 gagaggaagt aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc
     7741 tgctagcgga ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg
     7801 ggatcattgg tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac
     7861 ttcgcaagtt tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa
     7921 cacatttcgt acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa
     7981 tcaaaaagct gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga
     8041 accttttgtg aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg
     8101 gttatcaaga gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt
     8161 tggagatctg gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg
     8221 ctacttgtat caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg
     8281 ctatcatgag aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga
     8341 tggtgagatc ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc
     8401 agaagatgta aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc
     8461 tgcagtgaaa ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat
     8521 gccagagatc catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg
     8581 aatcaattac tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca
     8641 tagccccatg ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc
     8701 atatcgtgga tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga
     8761 aaactatggt aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga
     8821 acgatttgtt aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga
     8881 tcatttgaaa tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat
     8941 gtggacatgt atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat
     9001 cgctgttgat ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa
     9061 aacggtctct gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa
     9121 tgtttgttct aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta
     9181 gatacttatc gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt
     9241 cttgaggcaa tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat
     9301 gaagtaacag gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa
     9361 ttggatgagg ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc
     9421 ctgacagata tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc
     9481 catgaatcga aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta
     9541 gaacgtgcac tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc
     9601 atggttgtaa aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg
     9661 ctttctgttc cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta
     9721 ttctaattac aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga
     9781 atacgcggat acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga
     9841 tatgaaattt ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca
     9901 acactttact ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga
     9961 gaagtgactc atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt
    10021 ttagaagaaa aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag
    10081 ggaaattaaa tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa
    10141 agtaccgaga tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat
    10201 gaattgatgg gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt
    10261 ggttcaatcg atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag
    10321 acggaaacaa acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac
    10381 agagcggcaa gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt
    10441 tttcttttct tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt
    10501 atagcattag ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac
    10561 tttttgcacc atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa
    10621 gagtatcagc gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca
    10681 cttttcccac ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata
    10741 ttttttgtta cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat
    10801 tcttatcgtc aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa
    10861 tattctattt atccggcgta gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt
    10921 at
//
LOCUS       DAPCMT010000094        10483 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPCMT010000094 DAPCMT010000000
VERSION     DAPCMT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.096"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            23..646
                     /locus_tag="RD743_002012"
     CDS             23..646
                     /locus_tag="RD743_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563815.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="RD743_002013"
     CDS             1096..2145
                     /locus_tag="RD743_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0563816.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="RD743_002014"
     CDS             2142..3023
                     /locus_tag="RD743_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0563817.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="RD743_002015"
     CDS             3026..3796
                     /locus_tag="RD743_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0563818.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="RD743_002016"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="RD743_002016"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDU0563819.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="RD743_002017"
     CDS             5107..6270
                     /locus_tag="RD743_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDU0563820.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="RD743_002018"
     CDS             6387..6797
                     /locus_tag="RD743_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0563821.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="RD743_002019"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="RD743_002019"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDU0563822.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="RD743_002020"
     CDS             9370..9654
                     /locus_tag="RD743_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563823.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="RD743_002021"
     CDS             9814..10050
                     /locus_tag="RD743_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDU0563824.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="RD743_002022"
     CDS             10047..10445
                     /locus_tag="RD743_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDU0563825.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3651 a         1765 c         2224 g         2843 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atg
//
LOCUS       DAPCMT010000095        10413 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPCMT010000095 DAPCMT010000000
VERSION     DAPCMT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.097"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(36..932)
                     /locus_tag="RD743_002023"
     CDS             complement(36..932)
                     /locus_tag="RD743_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0563826.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(925..1605)
                     /locus_tag="RD743_002024"
     CDS             complement(925..1605)
                     /locus_tag="RD743_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0563827.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1606..2388)
                     /locus_tag="RD743_002025"
     CDS             complement(1606..2388)
                     /locus_tag="RD743_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0563828.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2381..3298)
                     /locus_tag="RD743_002026"
     CDS             complement(2381..3298)
                     /locus_tag="RD743_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0563829.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3462..3785)
                     /locus_tag="RD743_002027"
     CDS             complement(3462..3785)
                     /locus_tag="RD743_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563830.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4573..7800
                     /gene="fss3"
                     /locus_tag="RD743_002028"
     CDS             4573..7800
                     /gene="fss3"
                     /locus_tag="RD743_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDU0563831.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7913..8227
                     /locus_tag="RD743_002029"
     CDS             7913..8227
                     /locus_tag="RD743_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDU0563832.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8240..8614
                     /locus_tag="RD743_002030"
     CDS             8240..8614
                     /locus_tag="RD743_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDU0563833.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8624..8959
                     /locus_tag="RD743_002031"
     CDS             8624..8959
                     /locus_tag="RD743_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDU0563834.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9042..10382
                     /locus_tag="RD743_002032"
     CDS             9042..10382
                     /locus_tag="RD743_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDU0563835.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
BASE COUNT         3306 a         1974 c         2099 g         3034 t
ORIGIN      
        1 acatttttct actagttctc actaatcttg tataattaaa ccctccgcaa cttgacggag
       61 atggagaaga tgcccccgtc taatctagcg gtcatctctc ctcccattcc ttccaccaat
      121 ttttttgaca ggtaaagccc gattccgcta gattccgtat ttgtgcggct ggtattttcg
      181 gtataaaaac gctggaagac tttttcaaca gaggtttgcg ttcctggttt gataggattg
      241 gccactgtta atcgaatgaa tggatcctct tttgaaaatg tcacctttgc ttgtccatct
      301 gcataacgca ataaattctg tatgatattt tgcagcactc gcagcaacat gagcggatct
      361 gctaatacag ttgtatttat cgagtgttca gcgaacgcta cctcgatatt tttctcagaa
      421 aattgttcat aaaaagagaa aaatacttct tccacctctt tagaaaaaga aagcgtatgg
      481 agatccattt ctttttgctt cgtctctatt cgagcaagat cataaaactg atccgtcata
      541 ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat acttctcccg tttctccgta
      601 tcctctacct gttccagtaa ttgtacataa ccgttaatag cagtcaaagg agttctgaga
      661 tcatgagaaa ggccgacgat cgatagtctg atgttttctt ccatttcttg aaatgcttgt
      721 cctttttgtt cattttcatc caaccatgta ttcagtgcat ctactaggtc cattagattc
      781 ttttctcgaa attcgactga aagacgacca ccatattctt gtcgttcaga aagagtggcg
      841 attttcttag ttaatctctt gatttccctt tgcaggaaga aaataaaact tcccataaag
      901 ataacaacga gaagcaacag ccatttaatc aaatctaaac cccactcccc acaccgtatg
      961 aatatacaca gcatgtgcct ttgctagctt ccctcgaaga ttggaaatat gcatattcac
     1021 tgttttctca ctatcaaaat atggttcttg ccaaactgtt tcataaagat ttgctcgcga
     1081 aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa
     1141 atggacagcc acaccattca catttacatc cctcgactct ttgtccagta agatttcctt
     1201 gtaatagatg cgttgcgaca gatcttttac ttggacagaa gtctgatgac gaagttggat
     1261 gccgattcgt aacaaaagtt cttcgatatc aaatggtttc gtcacatagt catccgctcc
     1321 tgctgccaac aaggaaactt tatctctttg atcgcttttt gctgaaagaa cgatgatcgg
     1381 aacattcgat gtctgtcgga tttctgctaa caatgcttca cctgaaagcc ctggaagcat
     1441 gagatctaga aggatcaaat cgacaggata tgtgcttaac aataattttc cttccgtacc
     1501 tgaataagca tcaagaagtt tgtatctctg ttcaagaacc tcctccagta aggaatgaac
     1561 agcttcatcg tcttcgataa tcaaaatagt ttccagcagg accacctact taatgtcaga
     1621 tttttggaaa ataaacattc ctagagctga tgataccacg attgttacta gactgaccag
     1681 aatcattttc ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat acatgagcca
     1741 cgtttccgta aaccactccg ttagaaattc caagcctttg atttttgcga aaacaccggg
     1801 aatgaaggcc gttcccatga tataaagaaa agaaagaagc aaactgacac tggtacttct
     1861 agtgatgaat gtcaaagcca ctggaacaga taatgtccct aatgtcagca gaagcaatac
     1921 aggaaatact tttagaacat aatttccata gtttttgatt ccctcaactt caccaaatcc
     1981 gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc aaaaccacgc caagtgttgt
     2041 gatcaaaagc gtaacgagaa gtttcgacac ataataagtc attcgattcc gtcctaaaga
     2101 taacgtattg cgaatcgtac cgttttgaaa ttcttcccca atataaaagc acgcaaatgc
     2161 tgcgaaagcc agaagaatcc atgcagccat tttttctacc attaaaatcc ctgttgcacc
     2221 gttgatgact gtatccgttg cttggtttaa ttcattgccg tattttgtat gcatatacca
     2281 ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt cctagtgaga cccacatttt
     2341 acggctcttc catactttta ttttttcagc agttaataga ttaaacatgt ggattgcctc
     2401 cagttaattg taagaaataa tcttccagtt tttgcccaac taggtgaata ccatccacag
     2461 gaacttttgc ttctaccaat gcttgattga tggctgctac ttggtcaatt ccttcaaaaa
     2521 gctggataac acggctagat tgtacaaaat aatccctata accaagagag tcgagaaccg
     2581 ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca
     2641 attcttcttt cgataattct ttgatcaagt gtccttcatg caaaatacca tagtgagtgg
     2701 cgatctgtga caattcatct agcaagtggc tagaaagcaa gacggtgatg ccccgttctt
     2761 ttaccaaacg ttggatgatt tctcgcattt ctgccatccc tgctggatcc atcccattcg
     2821 tcggttcatc caaaatcaaa aattctggat tcgtgatcag tgcatttgca atagctaaac
     2881 gctggcgcat acctaaagag aaattctttg ctttcttttt ccctgtattt tccaatcgca
     2941 tcattttcag caggtcttcg atttctctat ccgacacacc gccgttcgct gcttggatac
     3001 gtaggttatc tcttgcagtt aattctggat aaagagcagg ggtttcaatc gattgaccca
     3061 tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa
     3121 tcgagatcaa acccataata gttctcataa aggtagactt acccgctcca ttttccccaa
     3181 tcaatccata gatcattcca cgtttgattt cgattgatac tttatctaat gctttggcag
     3241 caccgtattt tttggttata ccagttgctt tcaagaccgt ttcatttcca aaatccatac
     3301 ttatccttct ttcttattga tgagtgaagt atagttgacg ggtataaaga aagcagccag
     3361 aaatgtataa agaaagtgta aattttccaa ttgaatgtca aaaaaaacaa tcggacaata
     3421 cgtgtctttc ctattatcag gcagtttgtt atacgcttgt atcagtttcc tgattctttc
     3481 ttttcgacca aagtcgtttc tctgtattcc tcttcactga aaatttttgc ttctttcaga
     3541 tcaagcttga gtaattttac cagaccggtt ataaggataa ctatagcaag actccctccc
     3601 agaaaaatta gactgtctcg cagacgtggt tgcgttgtct tccaatcaaa gagcgcacca
     3661 aacagtgcta atccaagtgg actggccacc atggtaatcg ttgttatcgt tgacataagt
     3721 cgtccttgta tatgcgttgg aacttctcgc tgaagccaga ccatataaaa agcaaaagaa
     3781 aacattaatc cttaaaaacg gacttatgaa aaataataca tgccgtttgg gatttacaat
     3841 tagctaccaa ataagaggtt atgacaaaag acttttcaca atcccttctt ccgaataaac
     3901 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ttatcaaaca tgagaagcaa
     3961 tttttgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcagaagcg
     4021 gataaataat attgtttgat gatactttat ctttttcccg tttgtttata gatcgaatga
     4081 tattatagac caagaatcct acctattaac atagctaaaa acacaaaaaa ggcactttcc
     4141 aaattgtgga aagtgccaga aaagttagga ttaaggtaac tgcgctcagc attgcttcgc
     4201 ccagtacgat tctcgtaact cgtgaactcg aacgataatc gcaattaacg ttttgagaac
     4261 tgtgaagctt tacgggcttt cttaagacct ggttttttct tcgtatcatt gaataagttt
     4321 gaaaaacctt gatttataaa ggttttcccg taaatatatt ttattatatt tgattaaatt
     4381 tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc agtatatttc gtattttaga
     4441 ttaaattttt aggctcattc attctcaaat ccattctatt cagtcaataa aaattttaaa
     4501 aagactttat aaacaaagat gttcaagcga gcatcttatt tttttgtgct taatttgaaa
     4561 ggagaacatt tcatgtataa aaaacaaaaa aagaaattat gtgtattaac tattttgagt
     4621 ttggctgttc aagcaattgt tggagcaatt gctcctacga ttacttttgc tgatgaaatt
     4681 acccatccac aaacggtaac cgtagaacta gatttagcac atcaatatgc tgttgaagga
     4741 acttttagtg atggtcgtcc aatgtcagaa gtcactgttc cgcattatgc agtgtacaac
     4801 ggctttaaac aagatatttt ctgtattgaa ccaggagtcc cgatttacaa tgagttcact
     4861 cctggctatg agaaaaaccc gttaccagat atgtccgata aagccaaatt agtttccgtt
     4921 ttatggaaaa aagtaggtac agatgcagat actcatattg tggcacagaa gatgatctgg
     4981 caagaagtta atgggtatac ccttcactca ataaaacgat cagatggtag tgccgtaaat
     5041 atcgcagcca ttgaggcaaa aataaatcaa gccattgacg attatcagaa gaaaccaacc
     5101 ttccataata gtacggctaa aacagtatta gggcaatcga ctactgtgac ggatacgaat
     5161 aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg ccgcaaacat tgattatcgt
     5221 gtgaatggta atcagttgat aatcacccca aatgccaatt caaaagaaag tggtgtgctg
     5281 acgcttaaga aatctgctgg taccggaaca cctgtcgcct ataaaaaggc tggccaacag
     5341 actttaatgg ccggggcgat tgataaacct aatacctaca caatcaaaat tgatgtggaa
     5401 acggagggtt ctttaaagat caaaaaagtc gataaagaat cgggtgatgt tgtaccagga
     5461 acggttttcc atttagactt tggaaaaact ttacctgcaa aagacgtgac tactgacaaa
     5521 gaaggcattg ctacattgga tgagattcct catggtacaa aagtaaccat taccgaaaaa
     5581 tcagtaccgg cgccttatac gattgatacc acccccatga ctaccaccat taaagcaggc
     5641 gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag ggcaaatcat tctggataaa
     5701 agcggagttg aaacagggag tgatctttgg aatgacaatt attccttagc tggtaatacg
     5761 tttgccattc gcaaagacag tccaaccggt gaaatcgttc aagaaatgac cacggatgaa
     5821 aacggtcatg cggaaacacc aaaagagatt gctaatgcgt tggaattggg aacctattat
     5881 gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt tcaaacctgt aaaagtcgaa
     5941 ttgaagtatg ccaatcaaac cgtagcccct gttaccagta acgtaaaagg gcaaaatcaa
     6001 gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag atactgggga taaagcacaa
     6061 ggtaaggcag tctttgaggg tgctgaatac accttgttta ctgctaagga tggaaaagct
     6121 gttaaatgga gtgaggcttt taaaccagag ttagcgaagg gaacgaaagg ttctgatgaa
     6181 acggtcactt tgactttaga cgaaaagaac caagttgccg ttaaacattt agctatcaac
     6241 gagtattatt ggcaagaaac aaaagcgccc gaaggatata gccttgatga aacaaagtat
     6301 cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg ccgtgattac ccgaaatgtt
     6361 acggcaaaag aacaagtgat tcgctttggc tttgatttct ttaaatttgc tggatcagct
     6421 gcgggcacta ccgaaactgg cttcaatgat ctgaccttta aagtgtcgcc attggaaggg
     6481 accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag cttataacga gcaattaggg
     6541 tttgatggtt atggcaagtt tgaaaatctt ccttatgggg attatttact tgaagaagta
     6601 gaagccccag aaggttttca aaagattacg ccattagaaa tccgttctac ttttaaggaa
     6661 aacaaagaag actttgtgaa gagtgagtat gtctttacca ttaccgaaca agaccaaaaa
     6721 caaccaatta aaacggtgac cgttccttac gagaagctga cgaacaaagc attttctgtt
     6781 agtttgaacc gtttaatgct ctatgatttg cctgaggaag aagatagttt aacttctctt
     6841 gcgacttgga aagacggaaa caaagaatta acttctctgg attctaccga gctagttgat
     6901 aaattgagtt acaatctaca tgaaatcaaa gaagactggt atgtggtagc gcaagccatt
     6961 gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa aagccaaacc agtggtgatt
     7021 gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag gaacttggaa aattcagcat
     7081 aaattaaccg ccgaacaagt tttaaataaa actattgtct tattcaacta tgtgtatgaa
     7141 aataaggaag cttttgaagc aggcgataag ccagtagcga aggatgttag cttgaataac
     7201 caagcccaaa ctgtcagttg tacagtggaa caccatgttt ctatccaaac aaaggcccac
     7261 ctagaaaacg gttctcagac ctttactcat ggtgatgtgg tggatatgtt tgatgatgtg
     7321 tcgattactc atgatgtact ggatggctca aaagaagctt tcgaaacaat tctgtatgct
     7381 ttattaccgg atggtacaaa caaagaaatt tggaaatctg gcaagattga ttatgaagta
     7441 aatgataaag aattcaccaa aactgtgctt gcgaaaaaag tagataccgg aaaatatcca
     7501 gaaggaacca agtttacttt tgcggaaatc aattacgata aagatgggaa tatcaatgga
     7561 aaacacaatg aagatttgaa agaaaaatct caaaccttaa caccaaaaga agttccaacc
     7621 atactaagta cgccgaaaca accggaaaca ccaactgttc caagtggctc tcaagaatct
     7681 agtcccacag tgaagacctt ccctcaaacc ggggagaaaa attccaatgt tttactgttc
     7741 attggtttta ctttgatttt tgcgacggcg ggctattatt tctggaatcg ccggaactaa
     7801 ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg cgcgaccgcc tcctaaaaaa
     7861 gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa gaggaaaaga agatggaatt
     7921 gaaatttgtc gtgccagata tggctgaaac ctttggaaag ttaagttatg ccggtgaagg
     7981 agaaattata acagaaggat atggacggaa tactacggtg attggtcgca gttatcattt
     8041 gtattccagt aagcaacggg ccgatgatat tgaggtggta gtagctgcgg aagccggtga
     8101 aaaggatttt gatcaagacc aaccgctaaa agccgtgaat ccccatttgg ttgccaaagg
     8161 ctatgagatt gagaatcgtg ggtttactga ttacgtgttg tatgttgatg atttagtgaa
     8221 agcataggag gaaaagaaga tgagattaac agaaggtatt gtcgtagatt caggtttaac
     8281 atttgggaag ttgcgttttt ccgcattacg ccgagaagta cggaagcaaa atgaggatgg
     8341 aacaattggt aatgaggtaa aagaacgaac ctataattta aaatcctctg cacaagggcg
     8401 aatgattcaa gttagtatcc cagctaatgt acccttacgc gagtttgctt atgatgcaga
     8461 ggtagagttg gtcaatccaa tcgtggatac cgttgccaat tatgtttttc gagaaggaac
     8521 taccgttaat tggttcatta aggcggatga tttagtcttg aagcggcagc cgaatcaagg
     8581 aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag attatggaaa ttaatgttga
     8641 acatatttta gattgtctcg atcaatatgg aaaaggcgag ctgactgagg aacaactcgt
     8701 taaagcatta acctctgatg aaaaaatgtt tctgattata catcaaggtt tacttggaga
     8761 aaatagcgac agtgaagaag actctgatgt actagactgg ttaggagaag aagagtcttt
     8821 ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag gcagaatcca ttttggaaga
     8881 aattggtgtg gagatgccgg atgctattga aggcttttta aatcaattgg ttgaaacgaa
     8941 acaactcccc tcctcgtagc agtaaatgac tagcataact ttttgaaatt gcctaatgaa
     9001 atcctattgg gctttttcga ttggaggtgg aaaccatcaa aatgtacaaa ggacatcgca
     9061 tacgcgcagg agatcagcac ttggtttatc actttgttct tggctggcta ctcgctctgt
     9121 tcatcggttg gatgagcgtc ttttattttc aagagtttag acaatttgat atttctaagc
     9181 tatcactttc tactattgaa atcgtttggt ctataaaaga tttagtatgt ctgcttggaa
     9241 gtctcgcttt ttcaggagct atgatattgc tttatattca cttttttctt gatcattggc
     9301 gaagcttgtg gcatcgacaa aagctagctc ggatgattct agagaaccat tggtatgaag
     9361 tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa tagtagccga accagagaga
     9421 ctatcagtta cttccccaaa atctgttatc gaatgaaaga tggattactg tctattcgcg
     9481 ttcaaatttc acttgggaaa taccaagatc agcttttaaa attggaaaag aagttagaaa
     9541 gcggactgta ttgtgagtta gtagaaaagg aactcaaaga ctcctatgtg gagtatactt
     9601 tactgtatga tatgatcgcc aatcgaattg ggatagacga agtagtggca gaaaatggga
     9661 ctctgcgatt gatgaaaaat caagtatggg cttatgattc tttaccccat atgttaatcg
     9721 ctggtggcac gggtggaggg aagacctatt tcctcctcac cattatcgaa gcactattga
     9781 agtcagatgc agaattgttt atcctcgatc ccaaaaatgc agatttagct gatttaggta
     9841 cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc tgcttgtgtg gaagatttct
     9901 atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat gtctaactac aaaacaggag
     9961 agaattatgc ctatcttgga cttccaccca actttttaat ctttgatgaa tacgtggctt
    10021 atatggagat gttaacgacg aaagaaagtg cggtgatttt gaataagctc aaacaaatcg
    10081 tgatgttggg tcgtcaatct ggtttctttc tgattttggc ttgccaaagg ccggatgcga
    10141 aatatttagg agacggaatc cgcgatcaat tcaactttcg cgtggctttg ggtcgtatga
    10201 gtgaactcgg atattccatg atgtttgggg aggtcgaaaa aaacttcttt atgaagcaca
    10261 tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat tagtgagttt tatacgccac
    10321 ttgtaccaaa agggtatgat ttcttagatt caattagtaa attagaaaag atgaatgatt
    10381 gagtgatctt caatactgac tttatacttc aag
//
LOCUS       DAPCMT010000096        10394 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPCMT010000096 DAPCMT010000000
VERSION     DAPCMT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.098"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            256..531
                     /locus_tag="RD743_002033"
     CDS             256..531
                     /locus_tag="RD743_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563836.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="RD743_002034"
     CDS             544..2616
                     /locus_tag="RD743_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDU0563837.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="RD743_002035"
     CDS             2781..4667
                     /locus_tag="RD743_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDU0563838.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="RD743_002036"
     CDS             4679..5626
                     /locus_tag="RD743_002036"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDU0563839.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="RD743_002037"
     CDS             5646..6173
                     /locus_tag="RD743_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDU0563840.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="RD743_002038"
     CDS             complement(6236..6889)
                     /locus_tag="RD743_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDU0563841.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="RD743_002039"
     CDS             7021..7863
                     /locus_tag="RD743_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDU0563842.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="RD743_002040"
     CDS             8021..8917
                     /locus_tag="RD743_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDU0563843.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="RD743_002041"
     CDS             8920..9633
                     /locus_tag="RD743_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDU0563844.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="RD743_002042"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="RD743_002042"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDU0563845.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..>10394
                     /locus_tag="RD743_002043"
     CDS             10168..>10394
                     /locus_tag="RD743_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDU0563846.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
BASE COUNT         3606 a         1774 c         2152 g         2862 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tata
//
LOCUS       DAPCMT010000097        10311 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPCMT010000097 DAPCMT010000000
VERSION     DAPCMT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.099"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..277)
                     /locus_tag="RD743_002044"
     CDS             complement(<1..277)
                     /locus_tag="RD743_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDU0563847.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
     gene            605..1084
                     /locus_tag="RD743_002045"
     CDS             605..1084
                     /locus_tag="RD743_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563848.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            1117..3345
                     /locus_tag="RD743_002046"
     CDS             1117..3345
                     /locus_tag="RD743_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0563849.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3474..5117
                     /locus_tag="RD743_002047"
     CDS             3474..5117
                     /locus_tag="RD743_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDU0563850.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(5190..5735)
                     /locus_tag="RD743_002048"
     CDS             complement(5190..5735)
                     /locus_tag="RD743_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDU0563851.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5758..6243)
                     /locus_tag="RD743_002049"
     CDS             complement(5758..6243)
                     /locus_tag="RD743_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDU0563852.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(6233..7045)
                     /locus_tag="RD743_002050"
     CDS             complement(6233..7045)
                     /locus_tag="RD743_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDU0563853.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(7184..8716)
                     /locus_tag="RD743_002051"
     CDS             complement(7184..8716)
                     /locus_tag="RD743_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDU0563854.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8825..9856)
                     /locus_tag="RD743_002052"
     CDS             complement(8825..9856)
                     /locus_tag="RD743_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0563855.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3422 a         1909 c         1978 g         3002 t
ORIGIN      
        1 acttacattt aaatcgttgt ttcttcagct ccagataagt gcgatactca gacacatcat
       61 tcagtaacaa ccgaacggtt gtcgtccccc aacgaatgat cctagagggg acgacacaaa
      121 gacaatggga acactcttct ggcttgtaca gaagagttcc tttgtaaatc aagcaaattt
      181 gccctttgta tttttcttta gttaaacaat tttcctcgaa atagatattt aggtctagaa
      241 tatttagggt atctttgata agctgagtat gagacatctt gaatcctcct atgaattggt
      301 agccaaatta attctaaagg atttcagggt gtcttttcta tattttttta tacaaaaaag
      361 atgttggtga agttcaccaa catcaaaaag tatagagcca aaatattcca acatcatctt
      421 ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat
      481 caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt
      541 tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat gaggagttaa
      601 aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag aagactatga
      661 taaagttgat ggaagaaatg cattaaatat ggatgcaaaa gggctttcac tgggccttgc
      721 tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac atactggaga
      781 aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt tagcgatctt
      841 agctgcgcaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat tttatgatcc
      901 agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg tagaagtaga
      961 taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac tacaaaaaga
     1021 cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg agctaggata
     1081 ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc actaacagaa
     1141 aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa aagccatcgg
     1201 acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca agatcctgaa
     1261 atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac acaattagcc
     1321 aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt tgcagatatg
     1381 gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa ggaacaagac
     1441 caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact gacactgatc
     1501 cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga gctagttcaa
     1561 aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc tgaaatcgtt
     1621 cgagcgattg aaaaaggcta tttattagag attcaagagc cgactgtcat ccgagatgcc
     1681 tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca
     1741 ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg aaattaccaa
     1801 ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga aaaaatggat
     1861 cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa
     1921 gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc aaaagctaac
     1981 gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac ctataagcaa
     2041 ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc cactgattca
     2101 gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact ggaacttcag
     2161 gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa agaaagaacc
     2221 attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga aattctgcgc
     2281 ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa
     2341 gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag tagcgatatc
     2401 gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat gtctgcttct
     2461 gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc gcgacaaatc
     2521 atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc tttccaagga
     2581 aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga atcacttagc
     2641 cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa aggaaaatac
     2701 gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa ttttgacaaa
     2761 aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga tgaatctggg
     2821 tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa
     2881 ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga ccgttcgtca
     2941 agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca atggttaaat
     3001 gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc acttcgttta
     3061 gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa tattagtgac
     3121 ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct
     3181 gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat tggccaagac
     3241 aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat
     3301 gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcag
     3361 cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa caaggatact
     3421 tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac
     3481 aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag
     3541 atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga
     3601 aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata
     3661 atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt
     3721 ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca
     3781 tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc
     3841 cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga
     3901 catcgatttt cggcggttct gcttgggagt attctcatga aactggacag tattatctgc
     3961 atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac
     4021 tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga
     4081 tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct
     4141 ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt
     4201 acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg
     4261 tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc
     4321 aagaaaaaga agggcaatta gatgtcctta aattgaaaca tgcattaagc gcttggcaaa
     4381 cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg
     4441 ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca aaagcaatcg
     4501 ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa
     4561 tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt
     4621 ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa
     4681 caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt
     4741 ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac
     4801 aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa
     4861 ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt
     4921 atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat
     4981 cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa
     5041 attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa gctcggttat
     5101 ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt gcgaggaagt
     5161 ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc accgatatat
     5221 ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg
     5281 acaggtactt ggacatgctt gatatgcata ccgattgatg tgtcaccgat atcgataccg
     5341 gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa agcagcaaca
     5401 gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa attttttgct
     5461 tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc ttggacagct
     5521 agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca ttgaccaact
     5581 tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa
     5641 acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac tatttcagtc
     5701 aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct ttttcaatta
     5761 atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg caagtcccat
     5821 tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg gccaatcata
     5881 taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta ataaaagccc
     5941 taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc cttgtaaccc
     6001 gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat cgatcaatag
     6061 accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag cagcggtata
     6121 gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt
     6181 taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt gcttagtttt
     6241 catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta tgatcgttca
     6301 tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct actgctttgg
     6361 taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca caaccggccc
     6421 cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga tcaattgaat
     6481 agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc acaatgtttt
     6541 ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac gaagagggaa
     6601 gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg
     6661 gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct tcagtttctt
     6721 tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt atgaagtctc
     6781 gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca tctaatgaag
     6841 tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata gattccagca
     6901 taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt ccgaatagac
     6961 caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat ggatcagaac
     7021 cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt ataacgaaat
     7081 tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt aaaaaaacaa
     7141 aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac ttttgtgatt
     7201 ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc gggattgcat
     7261 caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc tcccaccatc
     7321 gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga gaatgatttt
     7381 tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa aagtcttcaa
     7441 tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc aaaaataggt
     7501 ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca taaacatttt
     7561 cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac agtaattgta
     7621 tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc cgagcgcttg
     7681 tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa ctctctagtg
     7741 tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga agaaataccg
     7801 catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa
     7861 aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta gcagtaaaac
     7921 gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga gcttccaaca
     7981 tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat cccatggaca
     8041 tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga aaagcgatta
     8101 atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc catcccatag
     8161 aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta tgggtcatat
     8221 aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt tgttccatta
     8281 aataaagaat aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt
     8341 ttgtctcatc atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg
     8401 ttgttgttgg tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa
     8461 cagctagaag gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt
     8521 tcactccaag atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt
     8581 aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg
     8641 tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg
     8701 ttatggtctc catcattcac actccattat cttttgttaa gtaatataca aattcaagat
     8761 aatcgtaaca taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt
     8821 tgttttatat ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca
     8881 tcttttccca gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat
     8941 ttaccgcgtc agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata
     9001 gttctggcga tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag
     9061 gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg
     9121 ctgttaactt aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat
     9181 tcagtctgtt ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac
     9241 gtttaagttc tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg
     9301 caaggttcct cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt
     9361 caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag
     9421 ctgctttggt tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc
     9481 ctttttgata caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt
     9541 aatagctatt ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag
     9601 tatgtcgata gattttagct aagaaattct gataatcttg tttttctaaa tggataatca
     9661 gctgatcatc cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat
     9721 cttttgcgta ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact
     9781 gttgaaagac ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc
     9841 tagttttgct gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca
     9901 tttcatcaaa tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt
     9961 actagctaaa agatatgaag taagaagata catttttagt tattttatta gtgcagtcat
    10021 tcttttgatc ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat
    10081 gaaatggatt tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag
    10141 aaaacatttt ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat
    10201 attgttggga taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg
    10261 aagatttatt ctatggtagg agcaattata ttgagaatta atcctttttt a
//
LOCUS       DAPCMT010000098        10180 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPCMT010000098 DAPCMT010000000
VERSION     DAPCMT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.100"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            102..2543
                     /locus_tag="RD743_002053"
     CDS             102..2543
                     /locus_tag="RD743_002053"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDU0563856.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="RD743_002054"
     CDS             complement(2873..3577)
                     /locus_tag="RD743_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563857.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="RD743_002055"
     CDS             complement(3764..4009)
                     /locus_tag="RD743_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563858.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="RD743_002056"
     CDS             complement(4026..4877)
                     /locus_tag="RD743_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDU0563859.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="RD743_002057"
     CDS             5043..6047
                     /locus_tag="RD743_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDU0563860.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6830..7387)
                     /locus_tag="RD743_002058"
     CDS             complement(6830..7387)
                     /locus_tag="RD743_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563861.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(7566..8177)
                     /locus_tag="RD743_002059"
     CDS             complement(7566..8177)
                     /locus_tag="RD743_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563862.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(8290..8547)
                     /locus_tag="RD743_002060"
     CDS             complement(8290..8547)
                     /locus_tag="RD743_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563863.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(9144..9422)
                     /locus_tag="RD743_002061"
     CDS             complement(9144..9422)
                     /locus_tag="RD743_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563864.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(9456..9707)
                     /locus_tag="RD743_002062"
     CDS             complement(9456..9707)
                     /locus_tag="RD743_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563865.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         3198 a         1892 c         1840 g         3250 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctcctta ttccgaataa atggtgtcca aaagcttgca tctgccagat
     6301 tatctcattt cttatgacaa gctaagcaaa ttatctccac caaataattt attcatattt
     6361 ttgacttgcg catactaact aattcttaaa ttttcataca aaatttctca atttccctta
     6421 acgagtaata tttatgtacg gtgattctca ctagtatccc aatcactttt taagttatcc
     6481 cgatttcaat tctacttcaa aaatagttgc ttttatgtat gctgttagtt gattagatgg
     6541 tcatttgttg agctaggtcg tttattttaa acaagttgtt tctttagctt atacctatct
     6601 cgtctaccaa acgcaagatg gcaaacacaa atgcatacgc atctgtattt gccactttga
     6661 ttcgtataaa gctaacaact attaactcct ctcagctaat agttgctctc ttttttattc
     6721 agattcattt ttgctgatca cagcctagta tttcaatggt tttgaacact aaaccaaatt
     6781 gttccattac aacattcact ggctgatgaa atggattagt agatttcgat tattcaaagc
     6841 gattttctat tggtggatcg tgttcccaac taataagacg gattccaaac ctatcgtaca
     6901 gatcttctaa accacttata aattttgcac ctatttcagg tgttttattg cgtggatatt
     6961 ctattgttac gccattaaat ggataccaat taggattagg ctctcttttt ttctgtacat
     7021 ctcccataga atagttagca ctttctggga cataatatat tcttcgacca tcctctaatt
     7081 catctaataa gatatttcgt gatcgtctat cacttgctaa atgtagacgc cacgtgcgat
     7141 accatgccat gctacctgca tctgcagaaa atccatcaaa taatgcaaca attgtatctt
     7201 ctatcacaat cctttcccca tctacttcat attctaatct aaaatcaaac tcccctcggg
     7261 taatttctgg tcttggtggc ttaggaccca atattattct acttaaaacc gcattcgttc
     7321 ccagatgaaa cacagctata acaacaataa tgattaatat tttgtttcta tttgataaat
     7381 ttctcattat tcgcacatca cctctacatt ctcacatatt agttcgtaat actcctttaa
     7441 tatcaaaata tctactggtc gtaataatat tcgctatgaa ttcctactac tcgtataaga
     7501 aaggccaaat catcagcttt tacttgctga tgatttggct tgtttctatt tatcgtaatt
     7561 gttttctaat caccaaaatt gttaacaatc ggttcagata tttcccagtt gattaaggta
     7621 attccgtatt gtgtcaaaac ttcttgcgct tctccgaaat taagtacagt tcttatgtat
     7681 ccactttctc ctaatataaa gtctacctct cttttaaaac cttcctcgag atttctaagg
     7741 gctatatgtg gttttaatga cattgattgt acattttccc ccatataata ttgcgcactt
     7801 cctcgactaa aatagattcc tagatcgtcc ccaaaaggta gatcttctct accactagct
     7861 aatctacttc tccatcttcg ttctcttttc atagaacctg cgctgaactc acacccgtta
     7921 aactcagcta tgacagtatc ttcaattatg ttaatctgtc catctagttc gtattcaatc
     7981 ctaaatggaa attctccata ggtgatttcc ggttcgggta ttttaggtga gattaatgca
     8041 aagaaaggaa atattaagct tggcaacatc aaaaaaactc ctcctatgat cacaattccc
     8101 gatgtaatcc aaaatataat ttgtacaatt ctcattgatc cattgtattc cattttattc
     8161 cccctattac tattcatatc cgcctatcta tatcgatctt ccaatgcaga gataattgct
     8221 tccatacctt gattttttac tgagttacct atgcgagata tcattctcca ctaaggtgca
     8281 tcatattgtc tatctaacat gattaattct ccaataggtg tatactcctc caaaaacaac
     8341 atttaatgca tctccctcga caacataagc taccatgttt gctgtatatg tgtttgagtc
     8401 taattcataa gcagataaat ttactgtcgc tggattaaat ataatcgcat tcgtatttat
     8461 agctactgaa tttgctgctt cagcaccacc ttttgaatgt ccgatcattg tgatttcaat
     8521 agtaggattt acgtggacag ccctcaccgt ttaccctgac cattatagaa atcgcttact
     8581 tttttgaact acgcaacaaa ggatacaatg ctcctaactt cactgtctcc atttttgtcc
     8641 actaaggaat ataaaaaata ggcattaatt catttaattc ggtcacaaca cgttaccttt
     8701 taaatcatta tactgtatct catacattat tatcaataaa ctattataaa aatgcaaaaa
     8761 atgctttatc aatctatttt gtaattggtc ataatatcat tgaaatcata ccctgtatcg
     8821 atttttccta tgaatctcgt ctatttgtga ttgacgaaaa agaggcaaga cagacactgg
     8881 aatggtaatt ttacccacta ttttgctcca actatttgta atcgtaccca cttttgaaat
     8941 accgagatga tcattttaat tgatatgctg attcccaagc aaaaccccaa gaaattaaat
     9001 gctctatcgc atctagcttc ttgggttttg cttatataaa ttccaacatt taaatttttt
     9061 atgctgttcg gtctcagaaa tgccgttttt gcttatatca caatatcaag atgtcaaatt
     9121 attcgtgttg ccgatgactt tgattaattt ttttcaaatt gtttattaag actaatgctg
     9181 tctttttgca gataccaatg ttcgaacttt ttaacattat catatgatcc tgcaagtttt
     9241 aacttcttac cattagatgt tatcaaagtt ataattaacc ttgccttttt cccattagga
     9301 tcttttattt tatgaatttt ctgcaggtaa tctctcctaa tggcagctat ttttacctca
     9361 tcagtgctga taaaccattg atcatccaca tacatactgc ctgttatgtt gtgtttttcc
     9421 atttcttccg agaacgaaaa gtttaaggct cgttcttact tccaaagttg aatgagtcca
     9481 tggattggac ttagtgtgag aattgagaac aacaataaga atccatatcc tcccgcaaac
     9541 atccctaagg ctatactgtc tctccatcca taagtaggat tagaagagaa aagagtcacc
     9601 gtttgtaata atatgaaaag gaataatcca gcacaaacac caaagataac aactccaaac
     9661 attgaatttt tatacaatga ccatttattt ataggctttt ctttcatttt acttccccct
     9721 actgttctgt tcacacggaa tgaattcaaa gttcataagc cctcctcttt gaacatatga
     9781 atacgacttg ctaactatac tttaaaaaag cttgttttaa gcggttatag gcgagttgta
     9841 atctctattc atctcattag caaataactt gccgaaaagt ctactgaaat taaagcagat
     9901 agtggaagaa ttatttagcc aggatagaca ctatttgtga aacacatgtc tttacttttg
     9961 aaattctatg atgaaaagcc ttggaataca gtagctctta attggtaata ctttttccat
    10021 tataaataac ctcttttcat tttatattta tcccctctaa aaataatatc taatctaata
    10081 ttttaaactt atatttccct cgctgtgcta atagagcatt actaaataaa tagaaactag
    10141 aaaaaagcat tacgcctgcc agaataaacc aattatttgt
//
LOCUS       DAPCMT010000099        10086 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPCMT010000099 DAPCMT010000000
VERSION     DAPCMT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.101"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(247..759)
                     /locus_tag="RD743_002063"
     CDS             complement(247..759)
                     /locus_tag="RD743_002063"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDU0563866.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="RD743_002064"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="RD743_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDU0563867.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="RD743_002065"
     CDS             complement(3212..3655)
                     /locus_tag="RD743_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDU0563868.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="RD743_002066"
     CDS             complement(3656..4444)
                     /locus_tag="RD743_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0563869.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="RD743_002067"
     CDS             complement(4441..5382)
                     /locus_tag="RD743_002067"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDU0563870.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="RD743_002068"
     CDS             complement(5559..6146)
                     /locus_tag="RD743_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDU0563871.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="RD743_002069"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="RD743_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDU0563872.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="RD743_002070"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="RD743_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDU0563873.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="RD743_002071"
     CDS             complement(9063..9998)
                     /locus_tag="RD743_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0563874.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAPCMT010000100        10039 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPCMT010000100 DAPCMT010000000
VERSION     DAPCMT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.102"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(505..729)
                     /locus_tag="RD743_002072"
     CDS             complement(505..729)
                     /locus_tag="RD743_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563875.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            920..3697
                     /locus_tag="RD743_002073"
     CDS             920..3697
                     /locus_tag="RD743_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDU0563876.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3778..4197
                     /locus_tag="RD743_002074"
     CDS             3778..4197
                     /locus_tag="RD743_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDU0563877.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4209..4679
                     /locus_tag="RD743_002075"
     CDS             4209..4679
                     /locus_tag="RD743_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0563878.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4696..5475
                     /locus_tag="RD743_002076"
     CDS             4696..5475
                     /locus_tag="RD743_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0563879.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5462..6283
                     /locus_tag="RD743_002077"
     CDS             5462..6283
                     /locus_tag="RD743_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0563880.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6300..7349
                     /locus_tag="RD743_002078"
     CDS             6300..7349
                     /locus_tag="RD743_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0563881.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7362..8366
                     /locus_tag="RD743_002079"
     CDS             7362..8366
                     /locus_tag="RD743_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0563882.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8999..10018
                     /locus_tag="RD743_002080"
     CDS             8999..10018
                     /locus_tag="RD743_002080"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDU0563883.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3365 a         1655 c         2093 g         2926 t
ORIGIN      
        1 actctattta cttctttttt aatttgttct tgcaatcttt tttgctacgt aatttttgta
       61 aaaaagtggg ttgagagaaa tctaattgat tttcaaagaa ggagtatgaa aatgagaaaa
      121 atatttattc gtttattgtg ggtactatct tagaggaata aataatagtg gagaaaacta
      181 tggtttggcg cagattctat caggtttgat ggaacgaaga caacctcccg acatcaagtc
      241 aaaagctact tgagattttg atctgaaaat aaaaaaagaa aacaaggtag gaacatcaat
      301 gtttctacct tgttttcgta atcaatggga aaaaactaag agtggtgtac gtaatcaata
      361 aaaaggatgg tgacaacagt acctctttac tctctgaaag catcataact cgtttttcct
      421 cattatgcca ataaattgtt gttataccac atataaggat ttgtaatagc taaaaacaga
      481 caggaaagtt gaaatctgtg aatcttattt tgtatggaag gatttataga acgcaaaaat
      541 caagataaag atgaataaaa atcctgtttc ggctatatat tgcgtatcta cgccaataaa
      601 acgagtttgt tctgtcatag ccatataaag caaaggcaaa aatacaacta cagcgagata
      661 agatccgatg atttctcgaa aagttttcca ggaagtatgt ttcctgtttt tatatttatc
      721 cgatttcatt gaatagtcat agcctcctta ttctttcata tgttgaaatt ccagtataaa
      781 aactggaagg aactgtacgt tttttagaat aaaaattata agggataagt aggctgctgt
      841 ctataataag atatattttt caattcttta ttaaagattt tattgacaaa atgaatccgt
      901 tttctatgat ggaggagggg tgaataagtt gttgaagaag gaaattttat gttacttaga
      961 gaaccagaca gcctttttcg atttagaaca tatgaacgag gtctttacag cacaaaggtt
     1021 agctgatcat tttggggtaa aaagaaacac agtcagtcat tatttgaacc agttaaatga
     1081 tgagcaacta ctagtcaaaa tcaattctcg accagtgatt tatttccaca aagaagcttt
     1141 tgaaaaacaa ttttttaagt tacgcacgaa tttttatttt tctatcgatg atctcaaaga
     1201 ggaacagcct tttttcgcgc aaccaaaaga cttattctct tttatgatcg gatacgacgc
     1261 cagtttgaaa gaaagtatcg aacaaatcaa aacagctctt tactatccag atggcggatt
     1321 accacttttg attacaggcg aaagtggcac aggtaaaagt tatttagtta atttggtcta
     1381 tcagtattgt ctgcttcatg atctgctaga agattctgca ccattcatca cttttaattg
     1441 tgcacaatat gcagataatc ctgaactttt gactagcaat ttgtttggac atgttaaggg
     1501 agcgtacact ggtgcagaag aaaataaaaa aggggctttt gaagcagcag atggtgggat
     1561 cttgttttta gacgaggtac atcgcttaac accagaaggt caggaaaaac tgttcaccta
     1621 tcttgatcaa ggtgtgattt atcgtatggg agaacctaat ttgtcacgcc ggatcaaaac
     1681 acgtctcttt tttgctacga ctgaagagat tactagtcat ttcttaacaa ctttcattcg
     1741 aagaatacct atccgaatcg aactacctgc acttaatcag cgtagtcgta atgaacggct
     1801 agaactgatt tactcttttt ttctcgaaga acaaagaaaa atggggagcc ctttgtcagt
     1861 cagtggacca gctttgtcac ttttaacaag tcagaaatta tccggaaaca taggagagct
     1921 gaaaaatatc gtcaaagttt cagcagcaaa agcatatgct gaacagcgtg aacagcctga
     1981 aatccatgtc acgatccatc atttgcaaaa agaactactt gcgcagccta ttactaaaaa
     2041 tacagcacag caaggaatct atattacgca agaccagacc ttagagcaat taatggagaa
     2101 gcaacagccc gaacagcagc gaatcgttaa aagttttgag caaatcctaa ttgatttcaa
     2161 gaaaaataac tgtctgctat attcttgcga aggaaaatta aagcaagaag tgattcaatt
     2221 atttgatttt ttgctgtttg agaccagtag acaagaaaaa cacgagctgc tggtttattg
     2281 gacacaatct atccgagaga cctttcgaca aatggaaact gcctatcaaa tcaaattcga
     2341 tggaaacagt gtctatgcct tgagttacta tctctatcag cgaagcacta tcaaatggtt
     2401 accggaagaa catgatatta tccaactaat caaagaatta gaacaacaag tttctcagtc
     2461 ttatccatct agttatcact atgtccaacg aattctggaa ttaagtaaga tcaaattaga
     2521 tattgaagac acaagtatgg atcggatttt actgacgatc tatctaaaaa aagcgaaatg
     2581 gtcaaaagaa aaacgactgc caaaagcgat gattgctgct cacggttatg caactgctag
     2641 cagtatggca aatgtagtaa atcgtttatt acaagaggat ctatttgaat catttgatat
     2701 gccactagat gtcacaccac agcagattgc ttcagaaatc cttgattatt gcgaaaacag
     2761 cgatgtttca aatggattga tcatattagt agacatggga tctctgaaag aaatccatca
     2821 gttttttaaa aaacagttat ccgtaccgct tttgattttg aataatgtga cgacaccttt
     2881 agcaatcaca gtaggggaat gcctgcaaaa aaatgtatca ttagaagaga tagcagaaga
     2941 gactgttcga cagatccagc cagaatggcg actgctctat ccagaggaaa acaaacccaa
     3001 agcactcatt actacttgct tcactggaat cggtacggcg gtccacttaa gtgaactgct
     3061 agaaaaaagc ttacctacgg cttgtcagtt gaaaatcatc ccatatgaat atcagcaatt
     3121 aaaagataag aaaaacagtg acccattatt ttccatatac gaagtcgtag gaatgattgg
     3181 caccacagat cctgatatca ccaatatcga ctatctttca ttagaagacc tcatctcggg
     3241 ggaaaagatg tctgtactag ctgaatggtt ggacactact atgaatgctt cagagaagga
     3301 aatattcaat cagcgcatca ttcgtaattt ttcattggaa aaagtactgg attccgtgac
     3361 tattctagat acagagaagg tgatgggaga aatcgatcat tttatgagag aattagaggt
     3421 gcagttgaat cgaagcatca gcaatcccaa aaaattagcg ttgtatgtcc atgtgagctg
     3481 tctcattgaa cgattgatcc gacaaatgcc aatcgaaacc tatcaaggat tagagaaatg
     3541 gaagcagtgt caaaaagaag ggttatctgc tattaaatct gcgtttagtg tcattgaaga
     3601 gcattatagt gtcatgatcc ccaattctga gattgcctat atctatgata ttttgagtga
     3661 agatacagag tttctctcga tagaagaaga attttgaaca taagcaagtt gttgccacta
     3721 attttggcat gcaacttgct tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg
     3781 gatagagaaa ttatcttagc ttcccatgga aaatttgctt cagggatatt agattcatta
     3841 gagctgatct atgggaaaaa tcattctatt actgtattcg attgctatac tgatgagaat
     3901 tttgatttag cggaaacagt aaggcagctt tttatgaggt acaaagataa agaaatcatt
     3961 gttctgacgg atctttttgg cggaagtgtc aacaacgaat tcttacaata tatcaatcgg
     4021 gatcatgtct atctcgttgc cggattgaat ttgccactat tgattgagct agtatctcgg
     4081 ttggacgctg aagtagagac aactgttttg atccagcagg tgctaactga ttcaaaaaaa
     4141 atgatccagt tttgtaacga gcggctcaat cagaaaatag aagaagacga attttaggag
     4201 gaagaaaaat gattgtattg acaagagtcg accatcggtt attacacgga caagtagctt
     4261 tttcatggac acaaacaata ggcgcagact gtattttgat tgctaatgat gatgtaccta
     4321 cgaatgagat acgaaagaca acgatcaaat tagccaagcc acaaggagtc aaactagtga
     4381 tcaaatcaat tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat aaatataagt
     4441 tattcatcgt agtggaatcg atcgaggatg catataagct ggcagaagcg tatccagaaa
     4501 tcaaaaccat caacttagga gggatcaagg caaaacctgg tactcgcagc atcggaaaag
     4561 cggtaaatat tttaccagaa gaggaggaac gcttgaaacg attaattgac aaacaaatag
     4621 aagtagaaat ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa gttttgtaag
     4681 aaaggaagaa cctcaatgat tgtacaagca gtcttattag gaattgttgc atttgtcgca
     4741 caatcagaat atgcactagg gacatcgttg ctttctcgtc cgattgtgac ggggctgttt
     4801 acaggaatcg tgttaggtga tgtaaaaact gggatcatta tgggcgcaac tttagagtta
     4861 gcatttatcg gatcgttctc agtaggtgct tcgattccac ctgatgttgt gaccggtggg
     4921 attttaggga cggcttttgc tattacagct ggagccggta cagaaaccgc gcttttacta
     4981 ggtttgccga ttgctacgtt gacattgata ttgaaaaata tttacttagg gcttttgatt
     5041 ccaattatga accataaagc agacaattat gcagaagaag gaaattataa aggagtcgag
     5101 catatgcact tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt
     5161 tcttatatgg tgggaagcaa tacgatcgga aatctattaa atatgattcc tgacttcgtt
     5221 caaaaggggt tgtcagtagc tacagggttg attccagctc ttggttttgc gatgcttgct
     5281 cgtttgctga tcaacaaaca agttgcacca tattttttct taggttttgc tatagcttct
     5341 tacttagaga ttcctgtgac cggagtagct atttttggtg cgattttagc agttgtggta
     5401 gtcaatatta tgaatgtccg tcaactacca cttcaaacaa ctgcagggga ggtagaagaa
     5461 gatgaagaat tctgagatca aaaacagcaa aataacgaaa aaagagctaa atcacgtatt
     5521 ttggcgttct tttcaaatgg agttttcttg gaattatgaa cgtcagatga atatggctta
     5581 tgtttatgca atgattccga ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc
     5641 agctttgaaa cggcatctag agttttttaa cacgacccca catatcgtta cactgatttt
     5701 aggaatcacg gctgcaatgg aggaagaaaa taaggatgat cctgaatttg atgtgactgc
     5761 gatcgatagt atcaaaacat ctttgatggg acctcttgca gggttaggag attctttctt
     5821 ttggggaact ttgcgattga ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa
     5881 tattttaggc cctatcttgt tcattttgat tttcaatatt ccgcacattc tgtttcgcta
     5941 tctagcaaca ggttggggat atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa
     6001 cggtatgatg gaaagtttga cattaggcgc gtctattatt ggactgatgg tcgttggagc
     6061 aatgactgcc acgatgatca ccatcaatat tcctttaaaa atcggttcag gagaaaatgc
     6121 tgtgactgtt caaagtattt ttgatgatat cgttccaaat atcttatgtc ttggagcatt
     6181 tggcatagtg ttttatctat tgaagaaaga agtgaaacca ttaacgatct tgatcggact
     6241 agctttcttt ggaatttttg gttcgtggat agggattttt taaaaaagga gcagagaaaa
     6301 tgtttacgat gcaagattat atctatgaag aaaaggaagt actaagtacg atcttgaaaa
     6361 agaatgattt ttcgacaaga gaacatatga aaaaaacagt gaatttactg atcttagcaa
     6421 cgggttcatc ttataacgct tgtttagcag cgaagccagc attggaatct tatggtgatc
     6481 ttactgtcga tattcaagaa cctttttatt ttttcaatta tggaaaactt tctccttcaa
     6541 tcgatactgt gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa
     6601 aaatgataca aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg caaagtccac
     6661 ttgctttaga agctgcacaa attattgatt taggtgccgg ggtagaatca gtaggcttcg
     6721 ttacaaaagg ctattctgcg actgttttac agttactact cttagggata ggaattggaa
     6781 taagtaaaaa taaaatcagc aaaaaaatag aacaagacta catgcaacag ttgcgcaaaa
     6841 taatcaacca tttaccagcg attattcaaa aaacggaaga attttttgat ggatatcaaa
     6901 gtttatttcg tttagcacag cgtttcgtca ccattggata tggtccaaac tggggaacag
     6961 ctaaagaagc agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt
     7021 tagaagctta tatgcacggc ccatacctgg aggcagatgc ttcgcatttg ctattcttta
     7081 ttgaagggga aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt
     7141 acgttggaga gactctcacc attaccacaa aaaaagcaag aaacgagaaa acacttggat
     7201 tagcaataga atgtgatgaa tatctgtctg tattagcgct tgtcgttccg tttcaactat
     7261 tcgcttataa aaccgcagta gctaaaggta ttgacttgaa caaaaagatt tttgaagact
     7321 ttgatacagt cttgaaaagt aaattataag aggtggaaat catgctgaaa ttcaatgaag
     7381 aagaacaaat caaagcaata aaaggcgcac tagctttacg tccgcaagta gaagaaatca
     7441 tcgataaact ttatgcggaa aaatttgacg ctgtctatta tctaggaatc ggtggaacgt
     7501 atgcttcctc gatgcaagct gtcacttata tgaatgggaa aagcaacttg cctgtctttg
     7561 tacagcatgc tgcagaatat tacactactg gaaacaaaag attaacaaaa gattctatcg
     7621 ttattttgtc ctctgttacc ggtacaactc aagaagtagt gaaagcagta gaacaaatca
     7681 agaaagtagg agcgacactg attggattta tcgataaagc gaacagtaag ttgtctcagc
     7741 tatgcgattt tgtggtcact tatccagctc ctggaacaga acaaatcaaa ttttttatgg
     7801 cagcagatcg attgatgtat ttgaatggag aattcgaagc ttattcagaa tactatgaac
     7861 agctcgaacg ttatttacca acagggttag tggaagcaga gaaaaaagca gatgcatttg
     7921 gcttatcatt tgcagaaaaa catcgacgtg atagtatgca ttattttatt ggagcaggga
     7981 atcaatgggg agctgtttat tcatatgcca tgtgttactg ggaagaacaa agctggcttc
     8041 catcgaaatc tatccatgct gccgagtttc ttcacgggac gttagagatc gtagaagaaa
     8101 caaccccagt cacattattt ttaggcgaag atgaacagag agtgttatct gaacgtgtgg
     8161 ctaaactttt gccaaaaatt tgcagcaatt atacattgat agacacaaaa gactatccag
     8221 tagaaggtat cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg atgcatgtag
     8281 tgacacaacg catcgatgct catgtggagc agttgaactg ccatccatta gagatacgcc
     8341 gctactatcg tcagtttgat tattaggata gactggataa aaagaatata aaaaataaag
     8401 agagcagcga taactgctcc ctttattttt ttaaaatatt ggcatcaatt acgttataaa
     8461 acacattttt tgcattatct acagttcaac ttataatctg tttaatggga catgtgggtg
     8521 ccaaccattt tttgttatgc aatagtacta tttagttgtt gcatggatcc gtagtaaaag
     8581 tccaagtaca acgagttgcg ccagtttaca tagcgttttg tatccagaat gttgactaaa
     8641 acaaaagttc ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg acctgacaga
     8701 tttagaaatg ttcaatctgt ctcttatgta ttttaaatca atactcacta ggagaagttt
     8761 cctaacctcc taatagtaat gctggtagta acattgtcaa aactgcttcc tcttaggaac
     8821 aagcaaagca taacgagtgt aaaaataaaa tgtagactat tgtaacatat cattgattat
     8881 ttcaagtaaa aacgataaaa gaagtaaaca aacacttaaa aacaatggaa attagtccca
     8941 actttaagaa atgtatacta taatggaaat tatcaattat ttatttggag gaatatccat
     9001 gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc actgctttag cacaaacatt
     9061 ggcagaaaac gggcatgatg tccgtatttg gggaaatgta cctgaacaga ttgatgaaat
     9121 caataagtat catacaaatc agcatttttt acctgatttg acgatcccag aatcgattat
     9181 cggatataaa gaattggcag aagcagtgga taacgcagat gctattttat ttgttgttcc
     9241 aacaaaagcg attcgttcag tcgcacagga acttattggt aaaatgaata ctaagccaat
     9301 cattatacat gcaagtaaag gactagaaca agatacgcat aaacgaatct cagaagtttt
     9361 ggctgaagaa attccagaag aaaaaagaca ggcaattgtt gtattatctg gtcctagcca
     9421 tgctgaggaa gtggcagtac atgatatcac aacaattaca gctgcaagta ttgatcaaaa
     9481 agcagcagcc tatgtacaaa aactgttcat gaatgattat ttcaggattt atacaaataa
     9541 tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg
     9601 cgctatccat ggattaggat tcggagatga tgcaaaagca gcaatcatga ctcgaggatt
     9661 ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat cctctgacat ttatcggttt
     9721 gagtggtgtg ggggacttga tcgttacttg tacaagtgtt cattctcgca attggcgtgc
     9781 cggtaacttg ttaggacagg gacataagct ggaagaagtt ctagaaaaca tgggaatggt
     9841 cgttgagggt gtggcaacga caaaagcagc agtagagcta gctcaacagc taggtgttga
     9901 gatgccaatt actcaaacaa tctataatgt tttgtataat ggagaagata tcaaaaaagc
     9961 agcaaaagaa atcatgttac gtgacggaaa aatggagaat gaattttctc ttcgttaaat
    10021 agatgatcaa tagttaatc
//
LOCUS       DAPCMT010000101         9881 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPCMT010000101 DAPCMT010000000
VERSION     DAPCMT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.103"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            278..787
                     /locus_tag="RD743_002081"
     CDS             278..787
                     /locus_tag="RD743_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0563884.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="RD743_002082"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="RD743_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDU0563885.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="RD743_002083"
     CDS             2322..3302
                     /locus_tag="RD743_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0563886.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="RD743_002084"
     CDS             3424..4842
                     /locus_tag="RD743_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDU0563887.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="RD743_002085"
     CDS             4913..6382
                     /locus_tag="RD743_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0563888.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="RD743_002086"
     CDS             6392..6859
                     /locus_tag="RD743_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0563889.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="RD743_002087"
     CDS             6895..7767
                     /locus_tag="RD743_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0563890.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>9881
                     /locus_tag="RD743_002088"
     CDS             7848..>9881
                     /locus_tag="RD743_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDU0563891.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFK"
BASE COUNT         3424 a         1432 c         1975 g         3050 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa g
//
LOCUS       DAPCMT010000102         9762 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPCMT010000102 DAPCMT010000000
VERSION     DAPCMT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.104"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..201)
                     /locus_tag="RD743_002089"
     CDS             complement(<1..201)
                     /locus_tag="RD743_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563892.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFN"
     gene            complement(217..831)
                     /locus_tag="RD743_002090"
     CDS             complement(217..831)
                     /locus_tag="RD743_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDU0563893.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(978..1661)
                     /locus_tag="RD743_002091"
     CDS             complement(978..1661)
                     /locus_tag="RD743_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDU0563894.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1770..2228)
                     /locus_tag="RD743_002092"
     CDS             complement(1770..2228)
                     /locus_tag="RD743_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDU0563895.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2312..4075)
                     /locus_tag="RD743_002093"
     CDS             complement(2312..4075)
                     /locus_tag="RD743_002093"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDU0563896.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4296..5756)
                     /locus_tag="RD743_002094"
     CDS             complement(4296..5756)
                     /locus_tag="RD743_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563897.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5964..6890)
                     /gene="glsA"
                     /locus_tag="RD743_002095"
     CDS             complement(5964..6890)
                     /gene="glsA"
                     /locus_tag="RD743_002095"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDU0563898.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6913..8340)
                     /locus_tag="RD743_002096"
     CDS             complement(6913..8340)
                     /locus_tag="RD743_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0563899.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8371..9585)
                     /locus_tag="RD743_002097"
     CDS             complement(8371..9585)
                     /locus_tag="RD743_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDU0563900.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2895 a         2036 c         1622 g         3209 t
ORIGIN      
        1 gttaaagtaa ttaaacaatc cgctaatcgt ttgatctggc atttctattt ccttaccatc
       61 taaagtaaca gtagtaacga caaaatggtc ttgcaatggc ggaacttcta agtactgccc
      121 ttgcagaaaa tcattcactg tttcatattc ttttttcatt tcttttgaat ctgtcgatgg
      181 tttatagctt atttttaaca taactagtgc tcctttttat tcgggacgtg caatcgtgaa
      241 aatttctcct attgctgcat aatcatttcc tgccaaacgg cttacagcat tcaattttct
      301 tggatcgatc cttcctgtct tatcatcata gactgtttca tttaaatgat atttctgtac
      361 ttctagcaaa aataaatcag ctgttacttt atttttattc cgtatctcga catgttcata
      421 taaggatgtt tcaaaacgaa cttttgcttc tgtcaatgct ggtactgaaa tgattactga
      481 atcagttaaa gtcaagtcag tcaggtacag ttcgctttcc tcattaggca gattggctgc
      541 tgttttattt acttcttcta cgttgtcctc atcaaggata tggataacag cttctcttgt
      601 ctcaagaata ttctttgctg tgtcttttac tgcatcttgt tttctctgga tcgccaagga
      661 taagatgggt cgattcgaac tgacgatatt gaagtaactg aagggtgcga tattcactat
      721 atcttttgct gatttagtcg ttactactgc tactggtcta gggatgactg aaccaatcag
      781 caacttgtaa ttttcacgtt ctgtcaattc tgaaggactc cgttcaaaca tggtttcgcc
      841 ttctttctat aaaattcgtt aaaaagcaga tcttgaagac aaaatgaaaa gtaatgcttt
      901 tttcatcttc ttagaatctg ctttaaacta tctatcaatt tttctttttt gtcaagaatc
      961 tgtgccgtgc gtgtttttta gttatttgct ttacttgcgg cttcgacata tcttttagtg
     1021 atcaattgcg gacgggtgat aatagatcca actacgactg aaaaagcacc taattcatat
     1081 gctttctcta cttgttcagg agagtcagta tgtccttcca taattactgg gatatcgact
     1141 tgttctatga tttgattcaa taaatcaaaa tcaggtgtct gcgtctttcg tgaatattct
     1201 gtatagcctg ctaaagtcgt agacaaaaga tcaaaaccat cttcttgcgc ttgaagcgct
     1261 tcctcaaaag tcgaaatatc tgccatagct aaccgaccgt gttcgtgaat catttggaca
     1321 agttcagcag ttgttttttc tgtcggacgt ttacgagaag ttgcatccag tgcaatgatt
     1381 tctgcttgcg tctccaaaag ttcttccact tctttttctg tcgcagtaat aaacacttct
     1441 gacccatcgt aatctctttt gataatccct attaccggta aatcaacgat tcgcttgatt
     1501 tcattgatat cttctgtcgt atttgctcga ataccaactg ctccgccttc ttttgcagcc
     1561 aaagccattc tccccataat aaaagaactg tgtagcggct cgttttccaa agcttgacaa
     1621 gaaacgacga ctccgccttt gatttgttcc agtgtatcca caatatctcc tccagtaatg
     1681 ttatataaaa atacagcttc atataaaaat gtaacccttt tctattcagg agacaataat
     1741 gagctatttc tttcagcaga cctttcttac taaaagaaaa ttacttcaaa ttcttcgtat
     1801 acgaccagct tctcttctgt aaattctatg ttcaacgctt ctttataagc atgtttaaaa
     1861 aactcagcat ggactttccg ccaataagct aatgtccgat ccccttctcc ttctttccaa
     1921 gcatgctctt tcgtgacttc actaaaaggc aagatggtca catttatatt tttgatgata
     1981 caaactggtt cattttttcc attcaggacc acgtctaatt gaccaacttt tggtaaaggc
     2041 tcattctctt gtttatacag atcgtaacca cttgatgtac cagtctttat cttttttaca
     2101 actagttcgc tcaactcgtc tgccattttt tctgtatccc caaatgaaaa gaaacctaat
     2161 ttttctgatt gataactagg atatttttct tggaactttt tgacatattc ttctacttgc
     2221 tgatacataa catccacccc tttgcttttt ataatgaaaa aaccatccag atactagtat
     2281 aaacaattat ctggatggtg agcataatga attattgaat attcaagaca cgcagactat
     2341 cttcaacgat ttgttttggt gttaaatgat aacgttcata aagctgccct acaggaacgc
     2401 tatccgtgaa ttctttctct gcaccaaaat ttaatacgtt cattgaagaa tcaccataat
     2461 aacgactgat attttcgcca aatcctcctg acaaactacc atcttctaat gtgatgacta
     2521 attcgtgtgt ttcttttaat ttatctaatg tttctacgtc attttctgtg atcgttcctg
     2581 gattgatcaa cgttgcttcg attccttttt ttctaaaagc ttttactgtt tcttttccta
     2641 attcaagaaa accgcctaaa gctaaaattg ctacttttcc tccattattt actgtttgat
     2701 aggctggaat actataatca tttgcgatag ctgtaccatg ttctacacca tgtgtaggaa
     2761 ttcggattgc tacaggctgc gctgtttgtt cgaacgccca atctagcata gagatcatat
     2821 cctcttttgt tgctggagca agatatttga tgttcgggat acttgataag taaggaatgt
     2881 caaatgttcc ttggtgtgtt cggtcactag ctgagatcgt tccgccaccg actaaaataa
     2941 tcactggatt ctcgttgatc gctaaatcat gtgataattg atcatatgcc cgttgcaaga
     3001 aagtacttga ttggaagacg actggacgta ctccctgaga agccaaagca gaagcgaaag
     3061 tgatagagtg ctgttcagcg atccccacgt caaaataatg atctggatgt ttttcttgga
     3121 atgttttcag tccaaaggcg cctggtatcg ctgcattgac tgcggcaata ggagctgtac
     3181 ttgtttgcat tttcttatcc aaataatcca tgatgatcat attaaatgtt tctccagaag
     3241 ttacttgctt tgcctccccg gtggacagat caaatggtac atgccaatga tatttcattt
     3301 tattatcgat tgccggctga taaccgcgac ctttttccgt atggacgtga agtacaatcg
     3361 gatgatcaat atcttttact tcttcaaata aacggatcac cgtttccaga tcgtttccat
     3421 tatccacgta acgataatca aatcccattg ctttaaatag gttattgctg cttttaccgt
     3481 tggtttgacg aagctcagct aagtttttat ataaactgcc atgattctca tcaatagaca
     3541 tctcattatc gtttactaat acgattagat tggattgtaa aacaccacca ttgttcaatc
     3601 cttcaaaagc taagccacca cttagggagc cgtcaccgat aaatgctacc acatttcctg
     3661 attctttctt cagatctctc gctttagcta aacctgttgc taagctgata gatgtagatg
     3721 tgtgccccac tgtaaaatag tcatgtttac tttcatgttg gttggaatat ccgctcactt
     3781 cacggtaatg tcctttttca aaaccatttt ttcgaccagt taaaagctta tgaccataag
     3841 actgatggga aacatcccaa acgattttat cctttggtga atcaaataca taatgaaacg
     3901 caatcgttaa ttctactaca cctagattcg gaccaacgtg cccacctaca tcgctgtcct
     3961 tttgcaagat tttttggcgc atctcttctg ccaactgttt catttcctca acagataatt
     4021 ttttgacatc tgcggggcta ttgattgcat ccaatacatg taatactgta ctcataatac
     4081 attctctcct ccgtactatc ttcttttaat gaaaatagat ttgttacact ttatctattc
     4141 tactacttag agttgactct aagtcaatag cattttaaaa aaatttcaga gaaagcttgt
     4201 tatataagta gatcttcgca cgaatcagga ctcaaataaa aaccttctac agacaattaa
     4261 aaagctttgt agaaggtctc ctaattaagg tctcatcaag aaatatgttt ttttaatagg
     4321 tgaagttcct gagcaatatg atacaaatgg atgtttgcac cttttaatga gattggatag
     4381 atctttgtct tttcagtttc taatccattt tgatagctag cagcatcgca caaaaccact
     4441 ctttttttcg cacatagaca ttcatcaggc aattcttcta atgaacgaaa taaaatagga
     4501 tttatttcag cgatgccttc ttccgcatac aaatgaaatt gggttcgata aaaaaattgt
     4561 ttgactgaac tttctgaaac aacaaaaatt tctatttcct gtctccccgc ttgttcactg
     4621 ttgatcaaag aaagactttt gacgaatttt cgaatgaaat tttcattcca tcttagttgg
     4681 atttgatttt tctttttcca ctcttccaat atttctacaa tttttccgta cagaagttgt
     4741 tctttttctg atagaagata gatcctttcc ggtaaaaata cttgtccatc tgcccacaac
     4801 gtacggacaa attgtaaaaa ggattgcttg aaaataaaat tttctgcatc gtcgatttca
     4861 gcgatactaa taatctgtaa aatcaattca ttcagtgata ggtcattgag aacaaaattt
     4921 ttataaacaa cttcgaaatc ttttcttaac agctccatgt tctcattggt ataatttcta
     4981 ctgttgaaca aagaaaaaat ataatagaca tcttcttctt gtaagtctag aattcctttt
     5041 tgctcttcca atccttgaca gactaattga aataacggct tcgtttctgc gcttttctta
     5101 tcttcttctt tcagtccaag gggaaatgcc tttcttccga ttgcgatctc tattcctcgt
     5161 atgataaagg acttttcatc ttccgaatat cttcttttaa aaaaatgagt ctctacataa
     5221 tcaatcaact cgttacaagc ttcttctaca tacggatttt tattttcata gccttcccaa
     5281 ttgacataac gtactaaagc tagcaagatg ttccgcgagt caaattctgg gataatgatt
     5341 tctttatttt tcaaataatc attttcttta aaaaaatcca atacgatttt tttgattgta
     5401 tatactttgc tcaaagaaat aaattcaaga tcagccagtt ctgtactcgt gaatatacca
     5461 ttaaaataat aacttaaaaa ctttaaaaaa taagattgac cgtacagaaa ctgtgtgagc
     5521 tcagaaagag caataccatg tttgaatacg agtttatact tatgcttctc tcttacttgt
     5581 tcttttatac aatattctag acactcggct agactttcta gatcgtgaag gatcgttggt
     5641 gtagtcaccc ttagagaaat agctgtttgt tcgatgtcta tttcttggaa ttcaaataat
     5701 tgttcacaca gatacacttt tctaaatata tttcgctcga tgtaattttc cagcattcct
     5761 gatacacgcc tccagttttt cttatgattt tatttaatca tatctgtaaa tcgtattttt
     5821 gtttcctttt tatttagaaa agccctttca aaaaccaata aaaaaagaaa ctgggacaaa
     5881 agtcaaacaa gctcacaatt tcagcctcat tgtaggtagc tttgagccgt ttggactttt
     5941 accccagcct ctttcaagaa tggttattca aagatgtcag cgtctagttc tttgactaca
     6001 tctttcaaca attggatacc agctgcgctg ttaccaaatg gatctagtgc cgggctgaag
     6061 acacctatgc cgtaacgatt tggcacagct gccatcaacc cgccgcctac accgcttttt
     6121 gcaggtacac caacatggac ggaaaactct cctgattcat catacaaacc agcagttgtc
     6181 ataattgatt tgacgattgt agcactttct tcagtaatga tttgtttgcc attccaagga
     6241 gcaattcctt tatttgccag cacagcagct aattttgcta tgcctgtagc ggtgacattg
     6301 agggagcatt gtctgaaata agcatcgagt atctccggta cgtctccttc gatcatttga
     6361 ttacctttca tatagtaagc aagagagcga ttgatatcac ctgttcttga ttcagaatga
     6421 tagatttctt cattcaatgt gatattagga tcatcacaga tctctttcat aaatgacaag
     6481 attcgctcaa atttttcatc tgcatcttta ccggcaatca acgaggttgt tgcaatcgct
     6541 cctgcattaa caaaaggatt cattggatgt ttgcgatgat tgatttccat attcatgata
     6601 gaattgaagg caaaaccggt tggttccgtg ttgattgttt gaaagacttt ttctattccg
     6661 ttgtctttga ttgctaataa caatacggga acttttgaca tgctttcgat ggcaaagcgc
     6721 acatcggcgt ctcctccctc aatcagttgt tttgtatcca aatcataaat cgccatacct
     6781 aattgtttgg gatccacttc agctaatgct ggaatgtaat ccgccacttg tcctgtttga
     6841 taatagtgga aattttttcc aatcaattgt tctaattctt ttacttccat ttgaataacc
     6901 agctttcatt ttttattttt ctgcatgctg gaatttatga cggcatgcgt agatcgtaaa
     6961 tggaataact aagacgataa gataagaaac aatcagaatc gtttcataaa tcccatgctg
     7021 tccagctgcg atactagcag gtggaacaaa tgagatacaa aaagcaaaaa gtgaagtcaa
     7081 aagacctaaa caagcaacga ttacttttac aacttttccg cctggaacct gataggctct
     7141 ttcaagactg tcatctttaa aaaccagctt caaatagccc gcgaaaaaca taaagtacaa
     7201 catcaggtag attacaacag tcaatgacat cgctgcaaga aatgaaagat tattcccccc
     7261 gccgcttaat gtaaagacgg ctgcccagat tgttgcaacg atcccatttg cgatcatcaa
     7321 atgagcagga acaccgtgtg tgttttcttt tgcaaaatat tcaggaagta atccttcttc
     7381 tgctgctacg tgaagtgctt ttgaaggaga ggttacccaa gaagcaattt ctccgatgac
     7441 acctaaacaa acaagaatcg caagaatttt cgcaatccat gcaagatttg gatcaatgtg
     7501 tcttaatagg aattctaacg attgaatcac ccctgtatta agagacaaac catgttgagg
     7561 tactgtagta gcgacggtca atccgccaaa agtatctaaa atgattgcca gaataaccaa
     7621 tacaaacatt gctaatggat aatccttttt agggttttcc atttcatttg catgactggc
     7681 agatgtttct acgcccatat atgctaaaat aaaggaaaca aaaacaacca gcgtaggcaa
     7741 tttcgaaaaa tcaggaatca atgttttcat agacagtatc gtttgtagtg gagcgcctga
     7801 agcgacataa tggatcccta agccaaacaa gactaatgct ggcaacaaga taccagctac
     7861 aaaaccgatc cgagcaatca atgaagtgta tttggttcct tttaactgtg agagtaaaat
     7921 agcccagaaa ataatcaata ggattgccaa tttcagatat ttattgtttt gtatagcacc
     7981 aatacccgtt gcataggata atgcaccagt taagaaatac agcatggtca agtagccaac
     8041 tgttatctca aaccattgga aaaagattgc ggcaaaaccg aatcgttttc ccaatgtgcg
     8101 actgacccaa gtatagacac cgcctttttc ccatcctttc accgttgcca tctctgctgc
     8161 gcacaatgcc acagggataa accatagcaa accgcctaat aacaagaaaa atactaagct
     8221 gaagcctgag gtcgcgaatg tgggatattc atataatgaa acgaccattg agatggtcat
     8281 agccaaaaag ccaaagaacg ttaatttttt tgcaggaatt ttcgttttgc tttgttgcat
     8341 gaatcttcct cttttctatt tgaatttgtt tcaattgagt ttgttgccaa agaagaaaaa
     8401 gtccaatcct tcttcttgtt cttcttttgt agtagtgatc gttgtaaagt gcatcgccga
     8461 ttttaaaatc actgttgtca gcacataggc atacacggca gtaatgatga caccaatcac
     8521 ttgaatccct aagaagtacc agctgcttgg attcgttagt aaatggctgt tagatgcaaa
     8581 gatccctatc agaatcgtac ctgtgaatcc gcccatgcca tgaacacccc acacatcaag
     8641 tgcatcatcc cattgtttct ttttacgaaa atcaacagca gcatgacaga cgatacccgc
     8701 aatgatccca atcagcatgg cactttttgc ttctacataa ccggcacaag gcgtaatggc
     8761 tgccaatcca gcaacggatc ctgttagtac gtcgacaaaa tcaaagccat ttccttttac
     8821 ttttgcaatg atcagccaag taatcatcgc aaatgcaaga gcgatgaatg ttgaaccaaa
     8881 tgcattgact gcttgttcat ctgctcgaag tgcaccaccg gaattaaaac cgaaccagcc
     8941 aaaccaaagc agtcctgtac cgatagcagc tgccataaga ctgctatgct gacttctttc
     9001 tcctaaagga atctttcttt gaccaagtgt gaggatacat gctaaagagc cgaatccagc
     9061 tgtcgtgtgg atgactgctc caccagcgaa atctctaaat cccatctgat ccaaaaatcc
     9121 gccaccccat atccagtggc aaacagggaa ataaatcagc agattccaaa aaataacaag
     9181 tataatatag ccttttaaat tcaatctccc tgcaaaagct cctgtcatca aaggaacggt
     9241 aatcacgata aacatcagtt ggtaaagaaa gaacattaaa aatggaatcg ttgcaccatg
     9301 aaacatgttg gggaagcttg taacatgtct catcaaaaag aaatctgccg ggtttccaat
     9361 aacaccgccg atatcccgtc caaaggccaa tccgaatcca ccaaataccc acataatcgt
     9421 tgttatccca acggagagaa aagattgaga catgatcgtt agtatattat tcttccgtgc
     9481 taatccgccg taaaaaaaag ctagaccagg agtcatcagg caaaccattg ctgtacataa
     9541 aatcatgaat gctgtatctc ctgtattgat aggtaatgta ttcatttttt tcgcctcctt
     9601 tctacgatta cgagtataaa agaaggtgta gttgtttttt tcttctgttt ttttgaatta
     9661 atacttgcat ttataaaaga cagacaatta ataaaagctt gatgtgttgt gctctttttc
     9721 gtttttcaca caaataaaga caaaaaataa acggtcttct tt
//
LOCUS       DAPCMT010000103         9680 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPCMT010000103 DAPCMT010000000
VERSION     DAPCMT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.105"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            34..771
                     /locus_tag="RD743_002098"
     CDS             34..771
                     /locus_tag="RD743_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDU0563901.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            785..1600
                     /locus_tag="RD743_002099"
     CDS             785..1600
                     /locus_tag="RD743_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDU0563902.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1613..2260
                     /locus_tag="RD743_002100"
     CDS             1613..2260
                     /locus_tag="RD743_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDU0563903.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2276..2932
                     /locus_tag="RD743_002101"
     CDS             2276..2932
                     /locus_tag="RD743_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDU0563904.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3094..3915
                     /gene="racE"
                     /locus_tag="RD743_002102"
     CDS             3094..3915
                     /gene="racE"
                     /locus_tag="RD743_002102"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDU0563905.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3918..5273
                     /gene="rph"
                     /locus_tag="RD743_002103"
     CDS             3918..5273
                     /gene="rph"
                     /locus_tag="RD743_002103"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDU0563906.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5274..5792
                     /locus_tag="RD743_002104"
     CDS             5274..5792
                     /locus_tag="RD743_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDU0563907.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5899..6399
                     /locus_tag="RD743_002105"
     CDS             5899..6399
                     /locus_tag="RD743_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDU0563908.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6679..7599)
                     /locus_tag="RD743_002106"
     CDS             complement(6679..7599)
                     /locus_tag="RD743_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDU0563909.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7764..8777
                     /locus_tag="RD743_002107"
     CDS             7764..8777
                     /locus_tag="RD743_002107"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDU0563910.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8887..9642)
                     /gene="fabI"
                     /locus_tag="RD743_002108"
     CDS             complement(8887..9642)
                     /gene="fabI"
                     /locus_tag="RD743_002108"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDU0563911.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         3130 a         1701 c         2081 g         2768 t
ORIGIN      
        1 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
       61 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
      121 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
      181 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
      241 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
      301 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
      361 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
      421 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
      481 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
      541 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
      601 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
      661 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
      721 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
      781 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
      841 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
      901 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
      961 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
     1021 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
     1081 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
     1141 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
     1201 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
     1261 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
     1321 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
     1381 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
     1441 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
     1501 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
     1561 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
     1621 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
     1681 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
     1741 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
     1801 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
     1861 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
     1921 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
     1981 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
     2041 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
     2101 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
     2161 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
     2221 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
     2281 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
     2341 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
     2401 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
     2461 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
     2521 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
     2581 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
     2641 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
     2701 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
     2761 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
     2821 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
     2881 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
     2941 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
     3001 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
     3061 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
     3121 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
     3181 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
     3241 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
     3301 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
     3361 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
     3421 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
     3481 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
     3541 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
     3601 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
     3661 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
     3721 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
     3781 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
     3841 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
     3901 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
     3961 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
     4021 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
     4081 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
     4141 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
     4201 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
     4261 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
     4321 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
     4381 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
     4441 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
     4501 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
     4561 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
     4621 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
     4681 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
     4741 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
     4801 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
     4861 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
     4921 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
     4981 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
     5041 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
     5101 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
     5161 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
     5221 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
     5281 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
     5341 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
     5401 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
     5461 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
     5521 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
     5581 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
     5641 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
     5701 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
     5761 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
     5821 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
     5881 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
     5941 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
     6001 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
     6061 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
     6121 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
     6181 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
     6241 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
     6301 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
     6361 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
     6421 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
     6481 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
     6541 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
     6601 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
     6661 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
     6721 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
     6781 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
     6841 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
     6901 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
     6961 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
     7021 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
     7081 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
     7141 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
     7201 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
     7261 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
     7321 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
     7381 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
     7441 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
     7501 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
     7561 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
     7621 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
     7681 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
     7741 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
     7801 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
     7861 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
     7921 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
     7981 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
     8041 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
     8101 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
     8161 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
     8221 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
     8281 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
     8341 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
     8401 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
     8461 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
     8521 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
     8581 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
     8641 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
     8701 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
     8761 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
     8821 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
     8881 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
     8941 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
     9001 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
     9061 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
     9121 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
     9181 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
     9241 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
     9301 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
     9361 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
     9421 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
     9481 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
     9541 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
     9601 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
     9661 ctgataaaaa tttgatagtc
//
LOCUS       DAPCMT010000104         9342 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPCMT010000104 DAPCMT010000000
VERSION     DAPCMT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.106"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..378)
                     /locus_tag="RD743_002109"
     CDS             complement(<1..378)
                     /locus_tag="RD743_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDU0563912.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKT"
     gene            complement(368..2542)
                     /locus_tag="RD743_002110"
     CDS             complement(368..2542)
                     /locus_tag="RD743_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HDU0563913.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2558..4900)
                     /locus_tag="RD743_002111"
     CDS             complement(2558..4900)
                     /locus_tag="RD743_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563914.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4922..7465)
                     /locus_tag="RD743_002112"
     CDS             complement(4922..7465)
                     /locus_tag="RD743_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0563915.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7475..7873)
                     /locus_tag="RD743_002113"
     CDS             complement(7475..7873)
                     /locus_tag="RD743_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0563916.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7954..8238)
                     /locus_tag="RD743_002114"
     CDS             complement(7954..8238)
                     /locus_tag="RD743_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563917.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8262..9260)
                     /locus_tag="RD743_002115"
     CDS             complement(8262..9260)
                     /locus_tag="RD743_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0563918.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2653 a         2002 c         1462 g         3225 t
ORIGIN      
        1 tgtttttctc tcaatttttt tgacgtttct atcatgaatc acgagaaaac ttgggacact
       61 tttaacatgg taacgttgta aaaaagtttg aagttcttct tgtcttggtt cttttcttgt
      121 ttcaattcga tatacattcc tagagaattt tttgtcgtat ctttccaaga aagattcaac
      181 ttcttggcat tttttacaat catttcttgc tagatacaca aaaaatgatt gattcgtttt
      241 ttgaagttct agtacttctt ctaaattact attgattagt tttccatttg attcttcgtt
      301 agtttttatc agtttaaaac aaccaaataa gaggaaacta acaataagta tcctaacaat
      361 aactttctta tttcgcatat tgtttctcca aatttggttt ccgaatatag acgactcgcg
      421 gtggttcctg gacgtcttca gctgttcgat acaccacaaa ttcgcctgtt ctcaatgctc
      481 gaatgcgatt cggattgaat ttaaagcggt ctacgtctcg tttcgtgcct ctttctcctg
      541 tccaatcaac ctttcccata tcagaataac ctgcttcttg tgtcgtcatt tccgttaatt
      601 caatgtcttc ttctgtcccc attaaatctg cccaataatc aatttctgtc gggtcatttg
      661 tcttcccaat aaagaacgta ttcacattcc caatcaactg ttttcgaagg acaggatcaa
      721 ttctatcaat gtccgcaagc gtttgagggg catacgccgc atgcacccca gcgcctcgtc
      781 ccttattgac taagtctata aggttatcat ttagataaga agctggttcg tcaaatatta
      841 ctaggacttt cgagcgattg gctgtttctc ctgcttgttt atacacttct gacgcaaaga
      901 aattaatatc tcccacaagc atttgagcca atgtccgaat aaactcacta taaatcaatc
      961 cgtttaacga gatataaaca atctttcctt ctttaacaac ttcttgaata tctaattctc
     1021 tttttcctcc agaagtatca aagagttcac caagctcact atccaataac atattgatat
     1081 tggtctgtaa attctcagaa gtcgcataca aataaaacgg gttcttctta tgttcgtaac
     1141 gaacaaacaa ccgtttaaac aacgtttgct cgatttctgt catgagagaa atgttttctt
     1201 ttaataatag ataatacgtt tctaagtcaa cggttttacg atcatgttca acgattcgct
     1261 cttcacttaa ctgttccaca tcggcaaaat cacgttctgt tttgtccatc gcttcattct
     1321 ttttcgtttt tttcttcgtg gttttcttct tttctacgac cactttttct ttaaataaat
     1381 ctggattttt caactgaata cgatccgcaa acaaatgtaa gacgtttcgt ggcaataaat
     1441 aatgttgtaa ccaaactaga tttctttcaa ttttatggct cttttggaac tcgtctaaaa
     1501 gctgaatcgt tacctgtaat aacgctttgg ctgctgtcga ataatacacg ctttctgttt
     1561 ctgctagatt ggttaattta tctcgaatgg caatatcatt gccatttttt acaggattat
     1621 aacgcaaatc gccaccgtcc acaaagactt caatcgggcg attgtatgcc tctgccactt
     1681 cttttaacgc ttgtaaagtc tctcctgctc ctttcccatc gactaagata acggggatac
     1741 gaaacttaca cgcatgttct gctagtaatt ggatcaccgt cgtttttcct gaacctgtag
     1801 tcgctggaat aatcacatgt tggttcaatt cactaaagtt catcacataa gggagcccat
     1861 actcatctgt tcctaataga atcactttgg ctaatgaagc cagttgttta ttccgcaaag
     1921 caacgtcttt tatgacaatt gctacctggt acgcttgctg atactctgct gtatattttt
     1981 gggactcctg aaatttattc ttccgacgtt ccttgtattc tttactattt agcaaagctg
     2041 tttgccgttc tttctttgaa acaacttttc ttttttcaat aaaagacgca tacgcaatca
     2101 aacaacttcc tgcacctgtc ccacctaaaa ggagggcaaa aatcgtttgt ccattaaact
     2161 gcaacgcatg agaaaacaag tttaatggaa ataataagta acacgacaaa taccctctag
     2221 gaagaacaaa aataaaacaa aatagattta atagcgtaaa acctaacgca aaaataaaaa
     2281 aaagagagct tcttcgtttt aagaagctct catactcaaa gtcataaata tgtgcacgtg
     2341 tctctttttt atctatcata aagacaatcg ctaaaggaat actgcctaca ataaaaaatg
     2401 gatatagaca aacacctaat aaagtgacga taaaataaag ttgttgaaac cgactttttg
     2461 gcgtttcttt ttcttgtttt cttttgcgat cgacagaaaa catttgctca aaatgtgtcg
     2521 gtcgttttgg tgcattcatc atactatcga tttcctccta tcgttttcgt tgtttcattt
     2581 ctcttcgata aatcggttgt ttttctcgtt ttcgtttcat ttttgcttga gttgctcgaa
     2641 actcactccg tgtcttcggt gcttgttttg gtgattttgt ttgtttcatg gttgagtatg
     2701 ctttagatac ggttgaaggc gctttggttt gtgctgttac tcgattgact gctgacatat
     2761 gttcccgaac cccctctgga cgtcgaacag atttcggtgt ggtagaacct gttgctcgtg
     2821 gtacattccc ttttcttagt ggtttttgta ccgcatgttt ttgtcctgca actgtccggg
     2881 ttttcatcga acttgttttt gttacgggag taagtgcctc cttccgacga cgacttgttt
     2941 gtccctgacg acgttttcga tcgttcaatt ctgttctagg cgtatgcttc tggccataag
     3001 acttgtgttt ttgctttgga ttcgctaatg acaccaccat tgcagaaccc tgattgcgat
     3061 acggactacg agacgcacct tttctacttc tttctcttag actaggattg ccttgcaatt
     3121 ctcgttgtaa taaatcggct gtttttcttg cctgtttctc cattgctgtt cgttgcatac
     3181 gttcttgact ttgctcttct gcttgtttcg cttgatcatg caagtctctt gctttttgtg
     3241 ccatacgttc catacgattt ggtgctttat gacgaggttg ttgtgcggat tcacgccgaa
     3301 aagcacttcg tgtggcttgt ttcgccgttt gtggcatacg aacgtctgag ccacccccac
     3361 gttttcgttg acgttcttgt ctcttccgtg cgttttcttt ttctttttgt ttctgtgctt
     3421 ttctcgcttc tttgcgttct ttcaaccgtt gtttggcttc ccttctcaat tttgcttctg
     3481 ccccaaatgg atttcccgca aaagctaacg ctcctcgaac acccattctt ccagtaaata
     3541 gactgcctaa aaagcctcgg aacaaataaa ttcccaacgg cgcaaacaaa taaacaatga
     3601 tcaagagaat tttttcccat ggatcgtctt gtgtactact taattggaac ccgtaactaa
     3661 gaaaagaaga aaaaaggata catgcaaaac ctgtcattcc ttttaaaaac gtcaaaatac
     3721 caaaaacttt ggcatagtta ccaaacacat tcattgtgac aagaaacaat cctaaaaaaa
     3781 gtaagaacgg caacatcggt actaacaata gttgcatagt tccaataatc aatcgaaaca
     3841 tacttaagac aaaataaaca acggtttgaa tcagattcac gacaaaataa aacaaggcaa
     3901 atgctgtatt tttccaacta ttgtaatatt gtatgttctt gtttttcagt gtatctgctt
     3961 catactctgt gactttgtca tgaattttct cccctatttt tgtacctaaa tcattgtcca
     4021 ataaaagatt gattcgatca tattcttttc cagcatattt gactttcttc tcacgaattg
     4081 tgtctacatt ggttgtgcca taatttaaca acagatatgg ttcataaaca ttactataaa
     4141 agacccgagc cgccatcaat ttgcctgccg tttccacttt tttatccgct gttacctctt
     4201 ctgtttcttt tgtttcataa tttgtttctt cttcaggtgt taacactgga ttgaccttca
     4261 cgaacgccgt ttccactgta gaatctaaag aaaaaagaac atcaaaaaga ttcttatttg
     4321 tcccagaatc ttttaatacg cttaacccag taaaaataag gatcgtcata agaaagacac
     4381 ggagtgcttg tttcattcga gcctctccag catatttgat tgccatcata attgccgaaa
     4441 aactgattcc aatagttcct gcaatactta acatattgtc cgcaattttt tgtgttaagt
     4501 tttgaatggg ttctcgaatg gcggaaataa tatctaattc aaagagaaat tcaaccattg
     4561 aagcattgaa ttctcctaac atttttacgc cactccaaac aaaatttttc atcactcctg
     4621 caacattgta cgatagcttt tttttaaggt tcattgactc atagtcattg gtcatcaatt
     4681 caaacgaatt ctttttgtaa tttttataca attgatccac atcagtcgtt acttctttta
     4741 cttctacttt atcgttggca ttctctcctt cagaacgatc ggtttcagtg acacccgcac
     4801 catcattcat ttgatcggca gaaacagtag ccccttgtag acagaaaaaa agacccccta
     4861 ataaaaggag cgtccaccat ttcagagatt tttttcgcaa aaaaaccacc tcttttttgt
     4921 tttatcgttg tttttcattt tctaacattg cttgaatttt ttcatcttga ttttcagtag
     4981 aagaaacagc caataaccaa ctatcaaaca acacatcaat ggcaatcggt tgatttcttc
     5041 ccaaatgatc ctggaataaa caggttcctg tttgtaattg ttccatcatt tctaaattgg
     5101 ctgaattcac ttccatatca aagaaggaca atagtttctc tcgtgcttct ttttgtttcg
     5161 gacgaaacgc aaatttgtaa gaaattaact cttttttatc ttcttgatcg taatctttaa
     5221 acgcttgcac aacaaggtaa atatccgtat tattcgcacg tccttttcgc aagctatcat
     5281 caatcaaata gcttccttgt gcggtatctt catacccttt tgcctcgtca taaataatcg
     5341 cagcgtcttc ttctttgttt gtagaaaaga tatacgtaaa cttcataatg acttccataa
     5401 ttgccattcc tgcaagttgt tcgctattta atcgacccga atcaatctct ttttgtgtcg
     5461 gaatgcgtat tccttgtgtc cctaacacgt tgatttgtga atcaaaacgt atcggcgtac
     5521 tgtgctcatt cccaatgaga atctttccta aaccaacgct atgccctaaa atcaatttag
     5581 ctagttcttt gtcacttttt cgaatttctt cgataacttg acttaacgaa tgcttacctg
     5641 gcatggtgta aactacatcg atggcgtctt ggatcaccgt tttcttgtgg ctcgctgttt
     5701 ctcgattggt atctacttct cctaacattc ctaacacatt tttagcggtt tgaattgcct
     5761 cttcttttgg taaaaacaat agaggatcaa gcatccctcg acagctttca tctgaagata
     5821 acgaaataaa attaattcgt tcatacatag ccacaaactc tggtatgtgt ccgaattttt
     5881 caagtgcctt taaaaagaac ttttgggttt catttttcgg atcaatataa agaatttttt
     5941 gacctaaaaa catcagccat aaaaaaatat attttacaag gaccgatttt cctttacctg
     6001 gtggaccaat aatcaacgta tttccattgg tatgttccgc gccttcaata cttttctttg
     6061 ttaaattggg ataataccat actagtttac tagatagcgc taacgcttct ttcgcagatt
     6121 taaacttttt tgcaataaca atccgtccta aaggaaatcc tttcttattt cctacttctt
     6181 tctttaagtc caagccataa tctgccacaa aacctgtcgt agcgacctgc tcgtaactac
     6241 gaaaacgttg cgtacttcca attaagcatt gattaaacaa cgttagctga tccgctaccg
     6301 atcgataaaa tttgaaatct gtagactcaa tcgcaaattc aatatctttg actcgttctt
     6361 ccaattcttc ttttgaagaa gcagatacaa ccagcgtgac ggtagaataa cacaatcgtt
     6421 tttctttgcg cttcagctta tcttttacat ccattaaccg ttctgtacca aaatttaata
     6481 catcgtctga atctaaaatg ccgtcaacgg tctcatattc tttttcttgt tcataaatcc
     6541 gtccagccat tttattaatt tttcgttcat cagattttga atgctcaaat cgaagtcgaa
     6601 tatgcgtatc aattggaaaa gataagctat cctgaagatt ttgaataaag ctactcccat
     6661 acatggctaa aggagcgtcc gtttgactta aaaacgtcga ataatgcgtt tgattcaact
     6721 gttcaatcgc aatgtatcct gcttcattcg aaatgacgcc ctcgcaaatt tccattggcg
     6781 taagctgtct ttcaggcaat ttttcttctg atcgatggaa aaaataataa taaatttgtc
     6841 caatctcttg ggcagataag cgctctatcg gcttataatt ggacaattcc gtatagagct
     6901 ccttttctaa tgtttcatac gttggtaaca cactacttat aggaactcgt tgtcccgtaa
     6961 tacgatagat aaacttttta aaaacatgct taagcaattg aaaatattcg atcggatcag
     7021 acactttcat ttctgtagtg aaacgcacaa acagatacgt tcgatactcg tttaattgca
     7081 cttcgtcttt taaaatcttt cctgcctgtt caaagtacac tttcccaaga tctgcgcatt
     7141 ctccctttac caaatctcga atggtttctt caatgtgcgt atctaggtta aattgacgtg
     7201 gaatattgat aaaatgatag tcatattcat catgtccaag tatgccttcg ccatcttgaa
     7261 gatagttctt gaaaaaatca atgtcattta aaggaaatac ttgggaagaa agttgatagc
     7321 ctacccacac atcatcattt ttggtcaaaa caaaatttcg gataatggcg tctatcggaa
     7381 attctaatcg aggaagcttc gtttttttct ttttgaactg ccaaatcccg ataataaaga
     7441 cgaccaaaag aaacaaaaac gccatttact cgactcactc cttccatttg tgtagggaat
     7501 acgtcacttt ttcatggaaa aacggcacat acatttcatg ttcaaagcgt tgtttccgtt
     7561 tctgataata ggacgttcgc ccaattaaat actcgaaaaa gtgtttattg tcccatttca
     7621 ttgaaaacaa tgtccaaacg agaacaccaa tcccaaaaat cataatcaac caggactttc
     7681 gaaacagtgt ctgcaagaaa ctaatttgat tcacatacgc aatcaaaaat aggatcaaaa
     7741 gaatgaagag aaatacccca cctatgacta attgttccat gcgaatgcca ttagaaatcc
     7801 agggaatttt ttgtccttta atcccgtaaa ttttgtgagg gtgcttaaac tcctttgtat
     7861 agttgtattc catgttcact ctacctcctt ttacaaaaaa agaagcgacc agaagttcta
     7921 atcttctgat cgcttttgca gtttgtcttt ttcttatgat aattgtatcc agggcacaat
     7981 catttgcgtt acccccacgt tctcttcact atccttaata aagataatgt tctttagcca
     8041 ttcaccaata tcaacggcat tcgataacaa taagacgagc attatcaaaa taaagacgcc
     8101 aataatggca aacgctgccc gcgcaaagcc ttgggtcgca taagcaccaa ccattagaac
     8161 aatcgcaacg attgccaata gccaaatgac ttgtccagat acatcatcaa ataacgattt
     8221 aagatcaaaa agtttcattt ccattctcct ctaagttcat tttattcatt taacgtagta
     8281 acaaaataac tatcgccact tgtttttact tccattgaaa agtttgattg gaactcgttt
     8341 cctttctcaa aatagaaacg atacttccct tgcaaaaagt agtttccttc tttatttttt
     8401 actgcatgat ccagttgtac attgatcaat tcgccgcctt ctaacccttt ttcgttagaa
     8461 atcaatgcca atttttcatc atttttgaca tataactcaa aaaaccgttt cacaaatgct
     8521 tccagcttga tttcttcgct ttcagtgacc gcttgcccag aagaataaaa tcgattttcc
     8581 tgatagttca cagaattggt attcttactc tcagaaagat tcaaaaattg aacgccatca
     8641 agtactttta aaacattgtt ttgataactg actggaaaag aaagttgact gcttttccaa
     8701 tgttctcctt ctgtaaaagt aacctcataa aataggcgat acattgcttg tgtcaaatcg
     8761 atttcttcta gtttaataaa gcgaacatct ttcgctactc gattggtagc cgtcgttaca
     8821 aagcctagat cagacgcttc caatccttgt gctaaataat gattgacgga agcctcccat
     8881 tcgtttcgtt tttctggcga ataagtaaat aacctttcaa taaaatgttg tccctcgtac
     8941 ttcaattgtt ccgtagaaga caaggtttgg ttgacttcag ttaacaatgt ttctggattg
     9001 acttgttttt tcactgctaa acgcgttaat gtatccactc gtccaaagtt taggaggagc
     9061 acgacaccca ttaacagaaa caaacaaaaa aaagcgcctg aaaatacact catggcactt
     9121 ttttgcgaaa attggatcat tcgatctgac ggttctgttt tctttttttg gaacaaaaaa
     9181 cgtttctttt gtttcttttc cttctgttgt ttcggtggct tagtttggaa cgcttgtgga
     9241 ttaaattttg cattactcat gagaaatgcc ataccgttct aacaataatt tcggtatttt
     9301 gtccacaatc actacttcgt ggacaaccac acttgccccc tc
//
LOCUS       DAPCMT010000105         9332 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPCMT010000105 DAPCMT010000000
VERSION     DAPCMT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.107"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            46..240
                     /locus_tag="RD743_002116"
     CDS             46..240
                     /locus_tag="RD743_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563919.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            509..808
                     /locus_tag="RD743_002117"
     CDS             509..808
                     /locus_tag="RD743_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563920.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(905..1339)
                     /locus_tag="RD743_002118"
     CDS             complement(905..1339)
                     /locus_tag="RD743_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0563921.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1571..2281)
                     /locus_tag="RD743_002119"
     CDS             complement(1571..2281)
                     /locus_tag="RD743_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDU0563922.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2271..3131)
                     /locus_tag="RD743_002120"
     CDS             complement(2271..3131)
                     /locus_tag="RD743_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDU0563923.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3136..3774)
                     /locus_tag="RD743_002121"
     CDS             complement(3136..3774)
                     /locus_tag="RD743_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDU0563924.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3789..4088)
                     /locus_tag="RD743_002122"
     CDS             complement(3789..4088)
                     /locus_tag="RD743_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0563925.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4131..5615)
                     /locus_tag="RD743_002123"
     CDS             complement(4131..5615)
                     /locus_tag="RD743_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDU0563926.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5635..6096)
                     /locus_tag="RD743_002124"
     CDS             complement(5635..6096)
                     /locus_tag="RD743_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0563927.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6114..7181)
                     /gene="ulaG"
                     /locus_tag="RD743_002125"
     CDS             complement(6114..7181)
                     /gene="ulaG"
                     /locus_tag="RD743_002125"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDU0563928.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7399..8169
                     /locus_tag="RD743_002126"
     CDS             7399..8169
                     /locus_tag="RD743_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDU0563929.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8442..9269)
                     /locus_tag="RD743_002127"
     CDS             complement(8442..9269)
                     /locus_tag="RD743_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0563930.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2893 a         1920 c         1472 g         3047 t
ORIGIN      
        1 tatatttgaa tattaactaa aaacagcaag aaagaagagg atcaaatgta ttatgtagaa
       61 gtaaaaacaa aaggcgtcaa aaacaaacaa tatgtgaaag gtatcagtaa cgaatatcct
      121 ttacttggtt catggaaaga agctgctcct ttttccaaac catgtgccat aaaaataaag
      181 aacgagctag aaaaagaact gacttgtggt aaagctgttg tagatatcat cgaaaagtaa
      241 atccaatgta tagaaaggaa gatcacacat gaaaagcgac gtacaaattg ctaagattat
      301 agaattagaa gaagaagacg cagtgttgac aattgaaaaa aaataaatgt ttgtatacca
      361 tatgattatt tcaatatgta tcctggaatt ggagatctcg tagagctata tcaagatgaa
      421 tcaaatatac ttgtcattca tcatgaaata tagagactta taaaattgtt tatttttata
      481 atgtatcagt atagaaagga aataaaacat ggattatact gttgaatttg acagtgaact
      541 acttaagcaa tttccacaag aagaacattt catgtataaa gaagcaattt cccacaaagt
      601 gacagagtat ttggagcata atttttttcg tataaagcca gtgcgtactc gttcggcaaa
      661 aggaatatac gagatgaaag tcaaaattaa taaaaaaaat tatcgcatag catttgcttt
      721 aaaaaaaaat catgtgttca ttttttatat tagttcgaat ttgcaaaaaa ttgtatttga
      781 taaagaggtg agtaaaaaaa taaaatagct gagcattgga ggatagattg agtgatacta
      841 agtctatcct cttgtttttc taaagaacta agctagaatt ttttcttttg tacgttatta
      901 tatgttattt gtcattacca aaaagcacct tttttcttct agtatctatg acataagaaa
      961 taccacccat ttcctttact tgaacaaatc ctactgattc ataaaatttt tttgttttct
     1021 cagtatcatc tgtcaataat tcgatttgga ctggtccata actagtgatc ttctccaaag
     1081 cttcattcaa aagcactttg cctattcctt ggcgctgata cctcggatga atgaggatat
     1141 cttgaataaa tgcgatcgtc gcaaaatcag tgatccaacg aacgattccg ataatcttcc
     1201 cttccccatt acgctttatc agagattcag attgttcaaa tgctctagct aaccttgcag
     1261 tgtcttttgt gtaattcgtc caaccaacgc tcttatataa ttcagaaatc tcatcgatca
     1321 taagggaatc aatattcatt tttttcttcc tttcttatac aattctccct ctaataatac
     1381 agttctttga ctatttaatc tatcacatat aactctcctg taaagattct ctcgattcct
     1441 taaaattcct ctttcctttt agaatgcata ctttttgata aataaaaagg ttgtgataaa
     1501 ttttcttcac aacctctgtt tctctctatc aagtgttatc ttttaatttt ctacccttac
     1561 cctagcatct ctattgcccg tagtatgcat actttccatg tttgcgaaaa taatgtttat
     1621 ctagtaaata ttgcgggatt tcacatatat tggggttgat cagctcgttt tctttggcca
     1681 tcagacaaat ttcatctaag atcaaactgt tttctactgc tttcataggt gaatcccccc
     1741 atgtgaacgg tccatgtcca tagacaagta ctccagggac ttcgtttgga tctaattttc
     1801 tttccttaaa cgtctcgaca atcacattcc ctgtatgaac ttcgtaagct tctcttactt
     1861 cttctttcgt taattggcgt gtacaaggaa ccttaccata aaaagcatca gcatgagtcg
     1921 tgccataagc tggcaaatcg cgtcctgctt gcgcccacat gacggagtgc gtggaatgag
     1981 tatgagtaat agcagttata tcttcaaaag tttgatacaa aacaacatgt gtggggagat
     2041 ctgaagatgg ttttaagcta tcttcttcta ttatatttcc cgacaaatct gttaccacca
     2101 tttgatctgc ttgcatgcat tcatatctga caccgcttgg cttaatgacg atgactccta
     2161 gctcacggtc cacttcactc acattgcccc aagtcaattt gactaatccg tatcgcggca
     2221 atgccagatt tgcttcaagg acacgttgct tcattgctgc aattttttct tcacaagaca
     2281 tcatagccag cctccttcaa tttagaactt aagtagtctt ttgcctgctg gatttctctt
     2341 cgaaagtctt ggcttttttc actccacatt tcaatcagaa atgttccatc ataatctaga
     2401 cgtttcaaat gtcttaataa tccaagaaaa tcaacacaac cgctaccaaa aggaacatca
     2461 cgaaactggc ctgaatattc ttttgttaca gggtaagtat cttttaagtg gatagcagtg
     2521 atataatcga tccctttctc aagttctcga gccggattat tttccggcca tgcggacaaa
     2581 ttgcctaggt ctggatatac ttgtagaaaa ggtgaatgaa tctgttcttt gatttctaaa
     2641 aattttgaaa tggagttcat aaatggatca tccatgattt ccacggaaag aatgatgcca
     2701 tatttagaag cttcctttac gctttctttt attccttcga taaagtcttc tctggaattc
     2761 attgatttct cttcataata aacatcgtat ccagctattt ggattacttt gacgcttaga
     2821 ttatgcgcca aatggattgc ttttttcatt agttgtttcg ctgctttttt cttttctgga
     2881 tcagcagaac caaacggaaa tcgccgatgc gcacttaaac aaatcgaatg gatacgtaca
     2941 ccggagctga acatctcttc tctcagctca gctatctttt cttcagacca atctaatcgt
     3001 gctaaccgct catccgtctc atcaatcgac atctcgataa aatcgaagtc catttctttt
     3061 acaagggaaa aacgttcttt ccatgaaata tctttgggta acgctttttc ataaatccca
     3121 atttgagcca tttccttacc cccagatacg aatgatctca tctttgaagt tttgtgcggc
     3181 cttcatcgga tcatttgctg ctgtaatacc acgaccagtg ataaaggtat acacatcgat
     3241 cccttcaaat aagtgtaatg tgtgtggatt taatccacca gttacagaaa cattaaagcc
     3301 tagttcaatc agttttttga tcttcgataa atctttttca ccccaagttt caccagataa
     3361 cagcgcatca cggctttgat ggtagattac ctgccggata ccgatgttat gccaatccat
     3421 ggcttgttca aaagtccaat tgccgtagag ttctacttgc aattcaccta tttctttttg
     3481 tgctgcctcc atagttggaa tcgttgcaca acaaatgact gtcatccagt ctgctcctgc
     3541 ttttgcaaca ttactcgcca ctgttcctcc agcgtctgca catttcgtat cagcgactag
     3601 ttttttatct gggaacatac tgcgaataca gcgaatcgct ttttgtcctt cctgtaagca
     3661 aagaattgtg cccacttcaa taatatctac tacatcacca actttcataa catctcctaa
     3721 tgcatgttct aagtcattgt gatctaacgc aatttgcaaa ttcggcctgc tcatcttagt
     3781 tccttccttt acttgttttt ttggtaccac tttgtttcag caaatttttc actgatttct
     3841 ttgactgaca tcacattttt gataccgata actgtaactc cttttttctc tgcctgagta
     3901 aacatattca aaaaattgac tggcgtaaaa actacatcgt attggccagc tgaacttttt
     3961 ccctctgaaa tggcacaatg atggattttg gtaattggta cgtttagttc tttcattgct
     4021 ttttccacac ttctcatcat catcaagctt gttcctgatc catttgcaca cgatactaaa
     4081 attctcatct taatccctcc attatgagtg ttgtctaatc agattttctt ttatactctc
     4141 tgcttttctg catatgtgtc atagtcttct acaatcaaaa aataaccatc aggatttttc
     4201 cgatattgta actgcggaat agccaataaa ccaatgacaa ctacggcaat tccagcataa
     4261 cctaggactt tcatgattac tgtaaacaat ggccaagctg ttgcccagtc gaacatcccg
     4321 atataaccac cgtatgttga catcccaatc catgtagcga ataatgctga accgcctact
     4381 tgtatgattc ctgaaagaaa cgggaacagg caagcggctt tgaatccacc tcggttattg
     4441 gcataaactg ctataacggc attatcaaag aacaacggaa tgaaaccggc tatgacgatc
     4501 gttggtgatt tcaataaaat cagtaaacca atcatcaaaa actgtcccaa agcaccaaac
     4561 aagaacccaa tcgttaccgc atttggcgaa ccaaagccaa aagtagcagc tacgtcaatt
     4621 cctggaacag ctcctggtaa aatcgtgttt gagattcctt ggaaagattg agttagttca
     4681 tcgacgaatg tccgaacacc taattgcaag atcgccagat ataccgcaaa atttaaagaa
     4741 gtcgtcataa tgtagaataa gaaactctgc ccttcttgca tgaattgcgc ttgaatcaaa
     4801 taatctttgc ctaatacaat cagaatgatc ccaaagaaaa atagcatgag taacgaagta
     4861 gcaaccatat tctcattgaa aatcgacaag aatcctggta atttgatatc ttcaattttt
     4921 ttgtcttgct tgattgatct atttttcttt tcatcccgct ttttcatcca ctcagctaat
     4981 tttgcaaata taaagattcc gaacatttgc tgatgtgcca ctgcaaatcc ggcaccttct
     5041 gtcaattcct gcgtgattcc tactgttaag ttagaagcta ctgtccaata acaacctaaa
     5101 atcagtccca taaaaatcaa tgcttgtatc ctacctagat caggaaagca aaacagtaaa
     5161 atccaaaaag cggtagctgc ttgttgaacc tgaacgtttc cggtagtgaa aacggaacgc
     5221 agtttcgtat atttctgaaa acggactaat aagatattta aaacaaatgc aatcagtaat
     5281 aaaatcatca catctccaaa ggtacggcca aatgtctcca ttaatccagc atccacagca
     5341 ttttgtccaa aataaggatc tgtaaccatg gcatccaaat tgaaccgttc ctttagtcca
     5401 accaagattg gccgaaaatt attgaccaaa ccaccagagc caacagtcaa aataaagtaa
     5461 ccgaccgttg cctttaagaa tccagcgatg cattcatgaa gcgggcgttt cagcaaaata
     5521 tagcctaata acacgataaa accaatcagg tatgctggct gcgtcaagat atttgttgca
     5581 aaatacgtcc aaatgcttaa caaaaattcc cccatctttt ttctcctcta tcaattaatt
     5641 atattctgcc atcacaactt ggtaatcatc gatggagttc gtagagatga gacgctcaac
     5701 caatccatct gtcattaata attcggataa tttttggata ttttctagat gttcttctgg
     5761 atcttttgct gcgagtgtaa aaaacaaact agccgttttc tcctcattct ccccttcact
     5821 ttcaaaacgg attggctgtt tcaacttggt aaatgaaata gctgttccaa aaacaccatc
     5881 acttttttct gaagaatgcg gcattgctac ttgaggtgcg atcacaatat aaggaccgta
     5941 tttttctaca caatggatga tctcctccac atacgtttca tcaataattt ccttatcgat
     6001 caatacaaga cagctttcgg cgatagcttc acgccaatcc gtgatttctt tatctgggta
     6061 atgaatcaat tgatggtcaa aaaagtattt cagcaagaaa ctccccttct ttcttataaa
     6121 aacgacggaa acggtgtatc attttcaatc gagaagacat cttcaaatcc tcgataaaaa
     6181 ttaaactcaa gatcatcttt gtttgtcgga tagatgaatt tcccgcctac ttgccagata
     6241 taaggtttaa atccatactt caggcgatcc tttttgaatt tccaaatttc agtgatctct
     6301 tttggatcag ccataaaatt agaccaaata tcgtagtgga caggaatcac tacttttgca
     6361 ttcaatgact ccgccatgcg cagcatatca acagaagtaa ctttgtctgt tatccctcgt
     6421 gggttttcac cataggcccc taaacagaca tcgattttgt gttcattacc atgtttggca
     6481 aataaattgg agtaatgtga atctcctgca tgataaagat tccctcctga tgtttcgaat
     6541 aaatagttta ctgcaatttg atccatctcc tgaggcattt ttccttttaa tacctcttct
     6601 ggatcttcac aagtgaccaa cgctgtccga tcaaacgctt ctaaagctac tacattgatg
     6661 tctttgattt tgactgcatc acctggcttt actgtaatcg ttttttcttc aggaattccc
     6721 catttttgcc acgtgcgaac cacttctttt ggcccaatga atgttgcttc aggacaattt
     6781 tgatgaactg ctgcggctgt atggatatct agatgatcgg aatggatatg tgtaacgatt
     6841 aaagcgtcta cattttttat cgcaaaagga tcgattacga aaggctgagt acgtaaatta
     6901 ggctgcatat tttgaacgcc actcatacgc atcatctgat ggcctttttt catcatgcca
     6961 ttcccatgac tacgttttcc cgtcccacac catagatcac ataaaatatt cgttccttca
     7021 tgacttttca gccaaattcc cgtacagccc agccaccaca tagcaaaagt tccaggatcc
     7081 actacttctg tttcgatttc ttcattcaag tatgtgcccc actcaggaaa tgtttctact
     7141 aaccactttt cttttgttac ttcattgaca gttttactca tttgcttgct ccccccatat
     7201 cgttgttcca acttacaagt aaatattaat gtgtatcagc atccatgaaa acccttttat
     7261 atgaccgttt gttttttata tgattttctt tcagtaatca atgattgttt cagtataata
     7321 aagacagata tgaatgttta aatgaacaac tagtcaaata aatccatgga tcaagatgtc
     7381 cctcaggagg caaaagtcat gaaaaactca cttgtcaata ttgaaaaacg tcaaaaagaa
     7441 attttggaac tgcttcagca gactgaacaa ctatcaactt tcgaattagc tgaatccctt
     7501 gatgtatcta ttagtacgat acgaagagat ttaaatttat tagaagaaaa aaacgagatc
     7561 atacgcaatt acggttattg ttcttataat tacaaaaacc agactgattt tgatcaatct
     7621 ggtcctgaaa ggattaaaca agcgattgcc agagaagcta gtaaatacat taacgattat
     7681 gatacgctgt ttatcaattc tagctcgact gcgttgaaaa cactggattt tcttcaatct
     7741 aaccatttga cagtcattac aaacaacgtg aaaatcaatt actctccgca caaagataat
     7801 tgtagttata ttttaactgg tggagagatg cgctttccaa aagaagcact agtgggcgac
     7861 atcgcattaa atacgatttc ttcagtcaat gcagacattt gtatcattgg ctgtagcgga
     7921 gtcgatgtag tacatggggt cacaacaaag atattaaatg aatcaacgat caacgaaatg
     7981 atggtcaaca agacaatcaa atgtaagatt ttagtcgctg atcatcgaaa aatcggactg
     8041 acatcaaagt tcaagattgc tgatatttca gtctttgatt atttgatcac tgataaattt
     8101 tgttctgaaa aagtattaga ggagatcaga aatacgggga taaaaaccat tcaagtagat
     8161 tcattttaag aaccaggacg taaatatgta aataggatta tcaaaaaaat gaataacaaa
     8221 tttatcgata aatgaaaaac acgctacgct caaattgata aaggaatccc aagatgtatc
     8281 catccaatcg ataccatcat aatctattgc ttcttttata accgttatat gttgcctaaa
     8341 taggaatcaa acaatatcga aaaaaggagg tatgacaaaa gtcacacctc cttttttcga
     8401 ataaacggta ttcaaatcag aacactgatt tcacaaccac tttatttcag gtcgttttca
     8461 atcaccttta ccgcgtaatc atttccgttg aaacgcgcta ttttttttgc gtccatatat
     8521 ctttctaaat aatcatcttc tcctaattgc ttatggcaag aagcaatttc ataaagcaag
     8581 tattccaatg cataagtcga tttatgtttc ttattatact caataccctt ctcacataat
     8641 tggattgctt tatcataatc ctcgatttta cggtaatagg tggcgatatc ataatataca
     8701 atgcagacca tcagattatc aaaatcagtc tcactcaata ttttaagtgt acgctccaca
     8761 taaactttcg caaaatctaa ttctcctttt gacaagtaga tcattgccac gtttgcagct
     8821 gcttgtaagt gatggatatc tgtttcggtc gtttgttcca acacttgatt aaaataaaat
     8881 aatgctgcat ctggtttttt cgcataaaga tgatcaagca gtccttgata aaataaaatt
     8941 tgatttaaaa gatcagaatt acttactttt tcctgatcga tcgtttgtaa aattttataa
     9001 gattcttggt attttgatac agaattcaat gattgaatct catttatttt tttcctgatt
     9061 ttgtcttctt ctgtttctac taatatatcg gagatctcga cattcagctt tttgcaaagc
     9121 gcacttaaga tattgatgcc tggcgacata ttcttgtttt cgatattgct gacagttgct
     9181 tgttcacaaa tgccttctgc taattgtact tgtgttaacc cctttttctt tcggtattct
     9241 tttagtaatg ctccatctac tttaatcaaa acaatttcct tccctttaaa aaattatttc
     9301 tcctttatat catacacaaa aaaaagttct aa
//
LOCUS       DAPCMT010000106         9281 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPCMT010000106 DAPCMT010000000
VERSION     DAPCMT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.108"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(12..680)
                     /locus_tag="RD743_002128"
     CDS             complement(12..680)
                     /locus_tag="RD743_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDU0563931.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            814..1101
                     /locus_tag="RD743_002129"
     CDS             814..1101
                     /locus_tag="RD743_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563932.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1517..3055
                     /locus_tag="RD743_002130"
     CDS             1517..3055
                     /locus_tag="RD743_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDU0563933.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3052..4017
                     /locus_tag="RD743_002131"
     CDS             3052..4017
                     /locus_tag="RD743_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0563934.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4004..5326
                     /locus_tag="RD743_002132"
     CDS             4004..5326
                     /locus_tag="RD743_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDU0563935.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5389..5493
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5567..6310
                     /locus_tag="RD743_002133"
     CDS             5567..6310
                     /locus_tag="RD743_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0563936.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6325..7317
                     /locus_tag="RD743_002134"
     CDS             6325..7317
                     /locus_tag="RD743_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0563937.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7329..8066
                     /locus_tag="RD743_002135"
     CDS             7329..8066
                     /locus_tag="RD743_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0563938.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8056..8778
                     /locus_tag="RD743_002136"
     CDS             8056..8778
                     /locus_tag="RD743_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDU0563939.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8862..>9281)
                     /locus_tag="RD743_002137"
     CDS             complement(8862..>9281)
                     /locus_tag="RD743_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDU0563940.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3037 a         1603 c         1963 g         2678 t
ORIGIN      
        1 aaaaataaga atcagtgctc taactgttca agaggatcaa gtggttcaaa tgtttctgta
       61 gtctgttttg cttccaattg gggcaaaaca gatccaattt ccagatactt cactttattc
      121 aatgtataag atagaataga acagctaagt aatgtataca gaaaatgtgt gagatccaaa
      181 taaagcaagg agacaactaa aataccaaaa gtaatcaaga aaagactttt gccaatctta
      241 atatgaaaat atttttcaat ggcttgataa atgatatctg ttccgcctgt gcttccgccg
      301 aatcgaaaga cgagaccgca accaatgcca gaaagaatac ctgtcatcaa actattgatc
      361 agtagactat ctacagcaaa atgtatatga aggctctccc ataaatgtaa aaagaaaact
      421 agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt ttccaaaaag aaacaatgag
      481 ataagcaata aaggtaaatt aaaaagcagt gaagtgacag aaggagcgaa tccaaaaaga
      541 ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg ccagcttaga tggaatattg
      601 attcctgtca cagctaatcc gtagattcct gcgcctaaaa tgattccgca caattctgta
      661 aataaattta attttttcat ttctagcctc ttttcttttg aaaattttca gcaacttctt
      721 tattatactg acttatactc ttacttcaag aggaaaatga aaaaaaatta tttttggctg
      781 tttcttttta tttatcaaat aaggagtgga aaaatgcgag cagcagactt attgacaatt
      841 cttacaaaat cacccaaaaa aacagagtat caaggagttt ttttggctca agaacaaaaa
      901 ctggtgcctg tgacaatggt ccagataaat aaagcaaatc aatttatctt tttatttgaa
      961 cacggcaaag caccacttgc tatgaaagac atctttcttt ttttgatgaa gaaccgtgag
     1021 aaagctgttt attatcagtc aaaagaagaa ctcattcctc tttatggagt gaaagagata
     1081 gaaaataaag tggtgatata agtaaaaata agaaaacaag acgtttatga tgtcaaaaca
     1141 accatttttt acatttttgg caaaaaagtg tcatgataca gtataatatt cgttgaacag
     1201 gccaataaag gtgttctccc ccacgagatt tctactttat tggctgtttc tttttattaa
     1261 aataaaagtt gtgtgtgtaa aaaagatata gctgattcca agtgacaatt tcgtatagat
     1321 ttttatggta ttcgtagtga aaaaacgaga accaaaaagt tgatccgact ttgttgcttg
     1381 gattcttttt ttgtcagcat atttagaggt atttttttct tttttttgtt cgtgctgtaa
     1441 ggttaaggca agacaagtta gttgggaaaa aggaaagaaa tgtttatact taaagaataa
     1501 cagaaaggaa ggtcgcatga ataattttat acaaacatta agtgaaagaa aaggcgagtt
     1561 gctggaagct gcatttcaac atctgtccat ttctctcatt tctttgctga ttgcagcact
     1621 gattgctatt cctttagcga tttgggcagc caatcataaa aaaatggcag aagtcttatt
     1681 gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc ttattaggtt tacttatccc
     1741 atttgtaggg atcggaactg ttccagcatt gattgcactt gttatttacg ctttgcttcc
     1801 gattttccaa aacacgtata ttggtctagc tgaaattgac ccgtctattg aagaagcagc
     1861 ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa gtagagcttc ccatcgcatt
     1921 acctgtcatt atctcaggta tccgaactgc cttagtttta atcatcggta cggctacttt
     1981 ggcagcattg atcggagcag gtggattagg gacatttatt ttgttaggta ttgaccgaaa
     2041 tacacctgtt ttgacattga ttggtgcgat cagttctgcc ttgcttgcaa ttatattcag
     2101 cggactgatt cgctggttgc agcataagaa acctcgatat tccatcatta ctttagcagc
     2161 agtggtgtgt ttatttggcg gcgtttcttt atatcagtct gccttgttta aaaatgaaac
     2221 catcacgatt gctggaaaat taggagcgga acctgaaatc ctgatccaaa tgtataaaga
     2281 attgattgaa gacaacacaa aaacagaagt tgaattaaaa ccgaattttg gcaagacgag
     2341 ttttttgttc aatgcactgg atcaaggtca aatcgatatc tatcctgagt tcacaggtac
     2401 tgtcctagaa agcttggtga aggtacctga agcaatgaaa agtgctgatt tgacaaaaga
     2461 aaaaacatat gacgaagcag aagaattatt gaataagcaa ttcgatatga cattattaaa
     2521 accaatggct taccaaaata cgtatgcctt ggcagtcaag agatcttttg cgaacgaaca
     2581 tcaacttaaa aagatttccg atttggcagc tatctctgat cagatcaaag cgggattcac
     2641 actggaattt attgatcgtt cagatggata taaaggaatc caagaagcat atggcttgaa
     2701 tttttcaagt gtacaaagca tggagccaag tttgcgttac caagcaatca ataatggaga
     2761 tgtcaatatt gtggatgcct attccacaga tagccagctc aaggagtacg atctagtgac
     2821 tctagaagat gatcgagggt tattcccagc ttatcaaggc gcaccgctga tgaaaaaaga
     2881 ttttgctaaa gaacatcctg aaatcgttaa agcactgaat aaattaagcg gaaaaataac
     2941 ggaggatcaa atgattgata tgaattacca agtcaatgtc gaaaagaaaa agccgacaga
     3001 agtagcacga atcttcctga aaaatgaagg attagtaaag gaggaagatc agtgactaaa
     3061 ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa agtatgtcat cgatgaccta
     3121 aatctggcaa tcaaaaaagg agaaatcttt gttttggttg gtccttccgg tagtggaaaa
     3181 acaactactt tgaaaatgat caatggatta tctaaaccat cagcaggaga catctacttc
     3241 aaaggaaaat cgttaaatga atataatctg cagaaaatgc ggtggaatat gggatatgtg
     3301 ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc aaaatattga agtcatccct
     3361 gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg tagatgaact tcttcaaaaa
     3421 gttggcttat ctcctgatat ttatcgagat cgtatgccta gagagttgtc tggaggagaa
     3481 cagcaacgga tcgggatcat ccgagctatt gctgcaagcc cagatgtgat tttgatggac
     3541 gagccattta gcgcattaga tccgatttca agaaactcat tacaagaatt ggttttgtcc
     3601 ttacacgaag aattgggcac gaccatcgtt ttcgttacgc acaatatgga agaagcaatc
     3661 aagctaggtg atcgtattgc atttatgaag gatggagaaa ttatccaatg tgacacacca
     3721 gagcagttat tgatgaatcc taaaaacgac tacgttcggc atttctttga tgaaccgaaa
     3781 cagacgaaag agtggcgagt agaagattta gtcgtcaatg gatatttctt aaatgaaata
     3841 ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta tgaaagaagt atattcgctt
     3901 ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa gaagtattgg gtcgctgaca
     3961 agtaaagaaa tctttgcttt tttaagtcga gaggaggaag gaaatgaaaa catatgatgc
     4021 aatcatcatc ggttcaggtg tgagcggctt aagtgctgct tatggattga aagaagcagg
     4081 taaaacagta ctagtggtcg aagaagatct atggggcggg acttgtccta accgtggatg
     4141 tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt aatcgggtaa aacaattgag
     4201 tggaaaaggt tttaacgaga tcccaactgc caactgggaa gaacttcaaa aattcaaacg
     4261 aacatttacg gatccagtcc cagaaagccg aaaaaaacaa ttagcagaag ctgaaatcga
     4321 tcatctttca ggaaccgctc gttttttgga tgattcatct attgaagtca acgaagaagt
     4381 cttccatgcg gattacttag ttttggcaac cgggcaaaga cctacgatct taccagtgga
     4441 gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca ttgcctgtac tgcctaaaga
     4501 aatcattttt atcggtggtg gttatattgc ttttgagcta gctacgattg ccaatgctgc
     4561 agggagcaaa gtaaccattg tccatcataa tcaacggcca ttaaaagaat ttgaggcgtc
     4621 tttggtagag gaagccgtcc atcaaatgga agcatctggc attcagtttg cttttggtgt
     4681 tgaaacccaa aagatcattt cagaaggtac tcgctatcgt ttagtcggaa aagagacgga
     4741 attagtagca gatatgattt tttgtgcgac tgggagacag ccgaatacag aatctttagc
     4801 acttgagcaa gcgaatatcg tctttgataa acacggaatt gcagtcaatg attatctgca
     4861 aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt tcaaggaaaa ccccaaaatt
     4921 gacaccagtc gccacttttg aaggaaatta tgtagctaag cggataacgg atgcaacaag
     4981 cgaacctatc aaatatccga ttatacctac gatcgtctac gccagtccta aacttgctga
     5041 agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa gtggtagaga tggatcttac
     5101 cagttggttc acctatcatc gtgtcaatga accagttgct aaggctgaat tgacctttga
     5161 ccagcaaaat tatttgattg gtgctgcagt gattagcgag caagcagacg aattgatcga
     5221 tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa gagttggata gctatatcat
     5281 ggggtatccg acattggcaa gtgatctctc ctatttattg aaataatcag catgataact
     5341 aaagcgcttg ttgctttttc cttcttgtgc tacactttaa aaaatgaata cctgtttctg
     5401 gggatgtcgt agacgacatg agagtaagtt gtgaacttag acccattgaa cctgtcagtt
     5461 aatactggtg aagggagaaa catagcagac gcttgaagcg ggttactatg tcgtgcccat
     5521 ttcaagcgtt ttttgttatt catattagaa gaaaggaggt aacaaaatga agaaaaatct
     5581 ttatccagca gtcagtatgg cattcatttt aattatttgg cagattgttg tcatagtttg
     5641 gaaaatacct gcctttattt tacccggacc tgctgctgta ttttctgcat tagcggcaga
     5701 tgcacaagta ttactttccc attctgtcac gacattaaat gaagcagtgc ttggtttggt
     5761 aatagctagc gttctttctt ttattactgc cttggcaatg gattattgga agtttttaga
     5821 aatgtctttt tatccgttac tagtgatttc acaaatgttg ccaatcatgg tgttagggcc
     5881 attacttacc ctatggtttg gtttcggtat gcttccaaaa gtgatccttg ttgtcttgat
     5941 aagctatttt ccgatcgtag tttctttttc ggatacattg aggaaagtat caaaagagca
     6001 aatcatcttt ttgaaaacga tgggggcaga tacagggaag atttatcgaa tatataaaat
     6061 accagcgggt ttatccggct tcttttccgg tttaaaagtg gctgctactt attgtgtcag
     6121 cggagcagtc gttggagaat ggctaagcgc tcaagcaggc ttagggtatt acatgatacg
     6181 cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg atcatttgtg tcattctatt
     6241 gagtttattg ctgaatggcg cttgttcatt attgaaaaaa gggtattatc gtatgcttta
     6301 tattttttaa gagaagggga tcaaatgaaa aaattatggc ttttattacc gctactgttg
     6361 cttttatccg cttgtggcac tgcaaaagaa acaagtagca aacaagaaat aaaggatttg
     6421 aaaaaagtga cacttgtttt ggattatgta ccgaatacta atcatactgg catctaccta
     6481 gcaaaagaaa aaggctatta taaagaggct ggtttaaatg tccaaatcat cgaaccagga
     6541 gataattcga caagtattgg tctagtaggt gcggacaaag cacaatttgg tgtaagttat
     6601 caagaagatg tcacttatgc tcacgcagat ggacagaata taccagtcaa agcaattgct
     6661 acagtgatca aacataatac ttctggattc gccaccctat cagacagtaa catccattca
     6721 ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa gtccaagtga agaagctgtc
     6781 ttaaaagctg tgatggaaaa agacggcggg gacttttcaa aactgacaat ggtaggaagc
     6841 aatggagaag ggccagaaag cttaggaaaa tcctcagata tccaatggta ctttgaaggt
     6901 tgggatatga tcaaagcaaa agaagcgggt attgaactaa actatatccc attgaaagaa
     6961 ttagatgaac ggttagatta ctatactccg gtaattatta cgaatgatca actgatcaaa
     7021 agtgatccag aattggtaca gtcttttatg gatgccacga aaaaaggcta tcaggaagct
     7081 attaaggatc caaatgacag tgctaaactg ttgcaaaaat atgcaaaaga aaatgatcgg
     7141 acgtttttag aagaatcaca agcattcttg tcaaaaaatt atacggatga tcctgagaat
     7201 tggggattga tggaagaaaa agtttggcgc aattatacag cttttatgca ggaaaacgga
     7261 ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta atcagtttat aaaataggag
     7321 tgatagatat gtcaatccaa atcagccact tgaatgtagt cattgaaaac ctgaaaatat
     7381 tagaagacat ccatttcact atccctaaag atacctttac atcgattgtc gcgccaagcg
     7441 gagccggtaa gtcaacattg ctgaaaactt taacaggagt ccagcctatg acttctggaa
     7501 cgatcaaagt agacgatcaa aaagtaactg gtctccatac agcatttagt tatatgccgc
     7561 aagaagatat gttacttcct tggctgagcg tctatcaaaa cgttacgtta tatcaaaaaa
     7621 tcaatcatct aacaatcaat gaagaacaag taagggatta tctggatatc tttgggttaa
     7681 acggctatga acattttttg ccggaacaat tatctggcgg gatgaagcag cgaacggcct
     7741 tgttacgaac aatcatgaat ccttcttctt acttgcttct tgatgaacca tttggagcgt
     7801 tagatgctat gaccagaggg cagatgcaag actggatgtt gaagctgcct aaaaaagcaa
     7861 agcggactac tttacttgtg acgcatgata tcgaagaagc aatttattta tcagacagga
     7921 ttcttgtgct ttccgcccgt ccggcacatg tgattgctga gattcaagtg cctgaaaaac
     7981 gacggtcacg tgaatggcta ctgcagcaat cagagttgaa gcaaacgatt tatcagttat
     8041 tggcaggtga attagatgtt aagtgatgct cattgtcatt tagatgggag tcagagttta
     8101 gcactccttc agcaggaaca cacgattttg acgatcatca attgtgattc gccagaagaa
     8161 tggaaggaaa acagacagct tgcagcaagt aagactcaag ccttaagtta cggtatccat
     8221 ccttggaaag cagatagcta tacgtttgaa caagttgagc cttttttgaa aaaagcaagg
     8281 atcatcggtg aaatagggtt agataatatt tggacgaatg taccgatgac tacacaaaaa
     8341 aaagtatttg agcgacagct tgcttttgca gccataaatg agaaacctgt tgtactgcac
     8401 acaaaaggat gcgaaaaaga gatcttaaat tatatccaag cttaccctaa tcactatttg
     8461 atccattggt attcttcttt ggactatcaa caagactata ttgatttagg ctgttatttc
     8521 agcattggtc ttgatttgaa aaagaatcca gcagtctggc aactagctaa agcagtccca
     8581 atcgaccact tacttattga aacggatgga ctacatgcag cagagtgggc tttagggggg
     8641 attttttcag aaaaagatta tgtctcactg cttgaagaac agttaaagac aatcgctgag
     8701 atcaaagagc tatcagttga agaagttaaa aaaaatactt atcaaaactt ggaacgtttc
     8761 attagattag gtaaataaaa aagagatgtg acacaactag tgtcgcacct ctttttggct
     8821 ttctttgtat aaaagtaaac tgtttgatta ataaatgaat cttagtacca accgtttgct
     8881 tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg aagtaacata ttgttctgct
     8941 actctttctt ggtttgcagc agagtagtca ccatttaagt aagaagaatc taattggtaa
     9001 cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc ttgattcttt ttgtgcgatc
     9061 cattcttttg cagagcttgt gcttgctgga gtagcagcag tttctgttac agcaggagca
     9121 ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt gaaccgtttc ttgtacaaca
     9181 ggttgttcag cttggactgg tgcttcttga acggctgctt gaggttcagc agctgcttgt
     9241 gcagagcctt ctgttgggat tgttaattgt tgtcctgagt a
//
LOCUS       DAPCMT010000107         9277 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPCMT010000107 DAPCMT010000000
VERSION     DAPCMT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.109"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..1049)
                     /gene="recA"
                     /locus_tag="RD743_002138"
     CDS             complement(<1..1049)
                     /gene="recA"
                     /locus_tag="RD743_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDU0563941.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1158..2399)
                     /locus_tag="RD743_002139"
     CDS             complement(1158..2399)
                     /locus_tag="RD743_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDU0563942.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2639..3217)
                     /gene="pgsA"
                     /locus_tag="RD743_002140"
     CDS             complement(2639..3217)
                     /gene="pgsA"
                     /locus_tag="RD743_002140"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDU0563943.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3426..4349)
                     /locus_tag="RD743_002141"
     CDS             complement(3426..4349)
                     /locus_tag="RD743_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0563944.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5088..6374)
                     /locus_tag="RD743_002142"
     CDS             complement(5088..6374)
                     /locus_tag="RD743_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDU0563945.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6367..7635)
                     /locus_tag="RD743_002143"
     CDS             complement(6367..7635)
                     /locus_tag="RD743_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDU0563946.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7887..8348
                     /gene="mscL"
                     /locus_tag="RD743_002144"
     CDS             7887..8348
                     /gene="mscL"
                     /locus_tag="RD743_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDU0563947.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8408..8854)
                     /locus_tag="RD743_002145"
     CDS             complement(8408..8854)
                     /locus_tag="RD743_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0563948.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(8947..9192)
                     /locus_tag="RD743_002146"
     CDS             complement(8947..9192)
                     /locus_tag="RD743_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDU0563949.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         2570 a         2009 c         1646 g         3052 t
ORIGIN      
        1 tattcttcta ctaaaggcaa ttcttcttgt cctttttcat cttctgggac agcgacttct
       61 tcaccaatac cataagctgc tctaactaga gcagaaactt ctgccatcat ttcaggatga
      121 ttagccatat agattttagc gttttcccgt ccttgaccaa tccgatcttc tttataagaa
      181 taccaagcac cgcttttatc aacaatgtct ttttctactg ccatgtctag aagttctcct
      241 tcttgtgaga tccctaaacc atacatgaca tctacttcag cgattttgaa aggaggtgct
      301 actttgtttt ttacgacttt gatctttgta cggttaccaa caatatctgt accttgtttc
      361 aattgttctg cacgacgtac ttccaaacgg atcgttgcgt agaatttcaa tgcgcgtcct
      421 ccaggagtga tttctggatt cccaaacatc acgcctactt tttcacggat ttggttgatg
      481 aaaatagcga ttgtttttgt tttgttgatc gaaccagaga gtttacgcaa tgcttgagac
      541 atcaaacgtg cttgtaaccc gacatgtgag tcacccattt cgccgtcgat ttctgctcgt
      601 ggaactaaag cagcaactga atcgactact acgatatcta cagccccact tgatactaaa
      661 gcatcagcga tctctagacc ttgttctcct gtgtcaggct gtgaaagaag tagttcatcg
      721 atattcacac ctaatttttg tgcatattgc ggatctaacg catgctcagc atcaatgaaa
      781 gcggccgttc cgccattttt ttgtacttct gcaatagcgt gtagtgcaac tgttgtttta
      841 cctgaacttt caggtccata tacttcgatg atacgtccac gggggtaacc gccgacacct
      901 aaagcgacat ctaacgcaag agagccactt ggaattgtag agatttgttg gtcgattttt
      961 tcgcccaatt tcataataga gcctttacca tagctctttt caattttttt cagtgcagca
     1021 tctaaggctg ctttacgatc atctgccaat acgataatcc tccttatatt tacaaatcag
     1081 agcaacattt cattatcctt atcatatctt ttttagtgaa aaaaagcaag aaaaaaccga
     1141 acaaatattc gcatttatca ttttcttaaa agctgttttc tcaaaagatc aaatccttgc
     1201 ataaccgcat tatatctgat agcagcacga tctctggcaa agtgatattt ttccgaaacc
     1261 acgccgtctt tagatgaaat agcgatccat actgttcctg cagattgacc ttctaacgtg
     1321 tccggtccag ccactccggt gaaagctaca ctgtaatctg ttcttgctat ctcacgtgct
     1381 tgaatggcca tctgctccac acattctttg ctgactgttc catattctgc taatagctcc
     1441 ggatcaatct ccagaaaacc agcttttgtt tggagcgagt aggtaacgaa tccgcctgga
     1501 aatacttcag atacaccagc aatgtcgcct aatgcggctt ggaaggcgcc ggctgtcaaa
     1561 ctttccgctg ctgttacagt ctgcttgttt tctttcagta gttccactac gacttttgct
     1621 agcgaattat catcgccgta tccataaaaa tattccccta ctcgttcttg gattttttct
     1681 tctaatgcta aaagagcttg attccctgcc tgaacatcat ttgttttcac tgtcagtcgt
     1741 aatgtcactt catttggttt ggcatatggt gcgatcgttg gattgatctg tgtctcaatc
     1801 agatctttta gctcagtgac cagtcgcgat tcaccaatcc cataaaagcg caaaacttta
     1861 gaaattagtt tttcttcaga aggaaaacgt tcctctagta aaggacgtac ttgttccaca
     1921 aacattggtt tcaactcatt aggcggaccg ggaagaagga tatacgcatg tgtatctgtt
     1981 tgataaaaag tccctaaagc aagccctgta cgatttggta gaggaacccc gccttcgata
     2041 atctgggact gttgcagatt gtttttggtc atttttcgat gggttgtttc aaaatatgcc
     2101 aataattttt tgtatccttc cgtattttgt atgagagact taccgaggtg agccgcagtt
     2161 gcttcttttg tcaggtcatc ttctgtcggt cctaatccgc cacataagag tatgagttca
     2221 cttcttgttt cagctagagc aatcaactct tctaaacgct gctgattatc tcctacaact
     2281 gtttgataat aaacttcgat acctagatca gctagctgtt ctgacaagaa agtggcattc
     2341 gtattcacga cttgtcctag taaaagttct gttccgacag ctattatttc tgctttcatt
     2401 acttcccgct cccttatata gtatacgctg tatttttata tttttttatt taaaaagagg
     2461 gcaaggagct gtgacaaaaa tcttgtcaca gctccttgtt ccaaataaac agtgttctcc
     2521 tgccaaaacc tcggtcataa gtcacaatat acataaaaca tgaaaagctg ttttttgaaa
     2581 ttatcccttg tgactcagag ttagacggca ggctcacaac ctctccacta aacgatactt
     2641 acattgatcc tttgaacaca ccgctgtttt tgatgaagta atccacacct gaatagatag
     2701 taaagatcag acaagcataa agcatgattt gatctactgg aataccgatc aggctgaatg
     2761 ggatattatt gataaacagc aagataatag caaccatttg tgtagccgtc ttgacttttc
     2821 ccggccaagc tgcagccatg acttctccat gttcgacaag tagtaaacgc aagcctgtga
     2881 cggctaattc acgacagaca atgatcgcta ctacccaacc aggggccttt ccttggtcaa
     2941 ccaacatgat aaaagcagtc ataactagca ttttatctgc caacgggtcc gcaaacttgc
     3001 caaaattagt gacaaggcct cgcgcacggg caatttttcc atctaaccag tctgtgatac
     3061 tagctaaagc aaaaataact gcacctacta gctgtgtgac agccagtgtg gtgtctccta
     3121 cgttaaattg tccccaatct aaaggtaaac ctacaaccaa aatgaaaatc gggatcatac
     3181 aaattctaag tacagttaat ttatttggta aattcacact atcgactcct ttcttcccta
     3241 ttctacgtga ctatcagcga agtttccacc gatattattt acttttccaa atgaatttat
     3301 tcaaaggagt atttttattg aaattgatct ttttgaatta acgaagaagg accgcgacac
     3361 agatttgtca cgatccttct tttttatacc tacaagtaaa cagatgcgta aactaattta
     3421 tacgatcatt ctgcgtattg aagagtaagg ttcacatttt ttggactctg attgttttcc
     3481 cctggattaa ctggtacgga ttctccattc gctttgatcg ttgcattgct tgctgcacca
     3541 atcgtgatgg tcgcatttgt tgctgtttcc ggtaacacgg tactctgtgt ttcatttgct
     3601 gccagtgtcc cttgataaac ataggcattg tctactagca cacctaacca tacacgattg
     3661 acagcagtaa attcaagggt tacaggcttg gtagcgtctg tgatatttac tgcgatggca
     3721 ctgccagaat cttctcctgt agctattgcc attttctttt cttcacttga ttttgttgtt
     3781 gattctactg tagaagactc tttggtctgc gtgctttgct ctgaggaaga ggactcctcg
     3841 actgatcctt ctactttaag cgaagaagct gtgtctccga ttattggatc agcttgacga
     3901 tcttgccaag tcatatagcc cacgacaacg acaatcgcca aagcaaccaa tcctaataaa
     3961 atgacaggaa gacttgtcca aaagcgactt ggatgcgatt cttcctgatg cattgctttt
     4021 ctagaagtac ttactgtttc aggctgcgga cgttcaggga ccgatcctgt caatttgctt
     4081 ttcccatcaa aaacatcgac taaatggtct ccatcttctc ctacagcttc agcgtattgc
     4141 cgaataaatg cccgcacgta aaatttacca ggcaattggt caaaatcatc attgtcgatt
     4201 gcttccaaat aacgtttttg aatttttgta atctgctgta attcatctaa agatattttt
     4261 ttattcaggc gtgcttgccg taatttttct ccgatggttt caaaagccac tcgttcatct
     4321 tctccagtct gtttgattct ccgaagcatc tgctgttgca acgaaagacg ttatgtttgg
     4381 gtgctgtgac aaaagtcatg tcacagctcc ttttttccga ataaacattg ttctcctgcc
     4441 aactcctcgg tcacaagtca caatatccaa taaatataag aagctgtttt ttgaacttgt
     4501 tccttgtgac tcggagttaa acggcaggct cacgacctct ttcttaaacg gtgttcaatc
     4561 agctgttcct cggtattagc tcaaaaaccg gcaaaatata aggagatatt tttccgtatg
     4621 tttctctaat actcaggtca aaacgctgat ttcacaacct cttccttaaa cggtgttcaa
     4681 aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa cattaagaag ctgtttttac
     4741 ttggctgttc ttattacttg atgcagggca gctttttcac aacctcttgg tacacggtgt
     4801 tccagaagta gcttcttcgg caataagccg aaatatcaca aaaatttgag aagcaatttt
     4861 cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct ttgattactt
     4921 cgtttgattt tattttaata taagttgatt aaaagagagc atgatttgtt ttattttctc
     4981 actaattttt ccaacacaac taagaatacc ataaaagctg attttggagg aggttcctac
     5041 tacaagtcta aaacgaaatt taagactttt cctcataaaa tactggttta ttttggtctc
     5101 atgtaaaact cgctgaaggc ctcttcactt ataaatgctt ccccagctgc cagtacgtct
     5161 ttcatttgga tcgaatcgat gatttcagga agatcaaata aagttcgatc gccaaataaa
     5221 ctttgcgtaa attgattggc aatatattcg atcgaattta atgattggaa atattgtcca
     5281 agcattttct tcttcagcaa atcgagattt gtttctgaca cttccggatc atcagcgaat
     5341 ccaagaatga tttctttgac tttttctgct gctttctctg gttcgtccgt atctccgctg
     5401 aagtccgcaa agtgaaattc acgatccaag ctgaattcaa aaccaaagct gtcatcgata
     5461 attccttgat tgtacatcgc tagatagttt cgagaagtat tgcctaaaat catttggaac
     5521 aacagattga ttgctgtttt atagcgtaac agttctgttc cttcttgcgg caatgtgtct
     5581 aatcctttaa taccaagtac aaatttatct cgtgtaatgg ctgcttccaa tgcactttgt
     5641 ttgatgattt cttttgtgtt ttcagggaaa taacgtacaa tttcttgctt tggagggaaa
     5701 ttttttgctt cttggttttc ccgaataagt ttcatcaatt tctcaggctc catcttacca
     5761 actacaaata aaaccatatt gcttggctga taaaacgtcc gatagcacgt atacaagtct
     5821 tgagctgtga ttttgtcgat actttcaacc gtgcccgcaa tatcgatatg caatgggtgt
     5881 gtaggataca agttatttag tatgccaaaa aacatccgcc agttagggtc gtcttcatac
     5941 atttggattt cctgaccgat gattcctttt tccttgttta cagtttcttc tgtgaagtaa
     6001 ggtgcttgta caaaatcgat cagagtcgtc agattctttt ctacctgatc cgtcgtagaa
     6061 aacagatagc tggttttcgt aaaacttgtg aaagcattgg cggaagctcc ttgtttccca
     6121 aataattgaa agacatcacc atcttctttt tcaaacaact tatgttctaa aaaatgggca
     6181 attccatctg gaactttcac cttttctttt tcaccatagg gaataaattc gttatcgatc
     6241 gaaccataat tcgtactgaa caaaccatat gtcttgtgat agtcattttt aggcaacaaa
     6301 tacacggtca aaccgtttgg cagtacttca tgataaagtg tttcattgat ttgctcatat
     6361 tctgttttat tcatttgcat ttcctcctgc taaaaagaag attgcctgca attcgatttg
     6421 ctcagctact tgttgtatct ctttgatcgt tacgctctcc atacgtttca accattcttc
     6481 atcagataat tttgtttccg gcagccagct gtccaaaaaa gaagcttcga ttgccgcttg
     6541 cggagagtct agagaaagca gatattggtt tctcagcatt gcttttgtct gagacagttc
     6601 caactctgtt atttcaccat ttcgcaaact ttctaattgc tcgtgaatca gatgaagtac
     6661 ttgatttcgg ttcttttcat cgataccggt ttggacactc atgaatcctc ggaacgtatc
     6721 cacgctacta gaggcataat aagctagact ttctttctct ctgacattca tgaaaagttt
     6781 agagtgagga aaaccgccaa ataaaccgtt aaagaccatt agcgcaaaac gttctggttc
     6841 atcgtaatac acatttgtct gataagctag gttcagcttt gcttgaacga ttggctcacg
     6901 tacttgtcgt tccttcacga tattcacttg aggttgggta taaaacagat ctggataaac
     6961 tgtttcccgc gactcaaatg gtaattgccc aactgcttca gctgctttgt ccggatcaac
     7021 atcgccgaca acaaaaatgt cgatttgatc atttgccatc atttcttgat agtaggacgc
     7081 tagcgaacta gcagtgatct tgtccaaatc ttcgactacg cccaaccctg ggatcttttg
     7141 ttccgggctg ttttcaaaat aaagttcttg caaggcatag gaagcaaaag tttgtttatc
     7201 ttccttcaaa ctttccaaat atagacgcag attgttcttt tccaagtcaa acgtcaactg
     7261 atcaaactga tgatttttaa tatttggata aaataaaacc tcttttaaaa aatctaccgc
     7321 ttgagaaaaa aggtttggat cattgacata ttcaccattc acaaaactga tccctgcgtt
     7381 tagccaatgt aaatttcctt tttttctaac tgataatcca aagcttgcgc cataaagctc
     7441 tgcaagttgc gcacttaact ttgtctgatc cgaataattc aaactatttg tttccaacat
     7501 gctgctgaga agtgaacgtt tcgtgatgac ttcttgttgc agacgcgcag taaaacggat
     7561 aaaaatccgg atcgttttgt attgttttgt tggtaagaca tgaagattca cgccttctgc
     7621 caattgatac accatattta tatgctcctt ttcacagttg attgcccgct tactctttta
     7681 atgggtcaac ctgccttttt tacttattcc tctgcttgaa atttataagt tcaggcaatt
     7741 cgccgttttt tctaaatgat gttacacttt atagtatagc atagatgttt tttatcaaaa
     7801 gaaacagacg gctaaaagcg tatattgttt ttttattttt tatttttcga tatactaaag
     7861 acaaataaaa gaagggaggg tcattcatga tcaaagaatt caaagagttc atcatgcgtg
     7921 gagatgtctt ggatcttgct gtcggcgttg ttattggtag tgcgtttaca agcatcgtaa
     7981 accaagtggt gcaaggcttg atcacaccac taataggttg gtttgtcgca atgatcacag
     8041 gtacttctga tttagaagga gcactttcaa ttcttgactg gtcgccgaca aaaggcgtca
     8101 catttgcgtt tggtaacgtt attagtgcta tcattacatt tctgatcact ggttttgtct
     8161 tattcttagt tgtcaaagcg gcaaaccgtg cgaagaattt acgctccaaa gaagagcaag
     8221 tggaagtgga agaaactcct actgcagaag attatcttag tgacatacgc gatcttcttg
     8281 ccaaacaagt ggcagaagac aatacacatc cagtagagca aacaaatctt tccgatactt
     8341 ctaaataata aagaatctaa aaaaaaagaa agccgatttt cagtgtcggc tttctttttt
     8401 tagattctta acttgctact tgttcctttt tcatgtcgat atgtggaata tcatcttcca
     8461 aatacacatc tgagattgcc ttgaatccaa acgactcata aaaagcttgt aggtattctt
     8521 gtgcgccgat cacgattggt ttttctggaa aatttttctg gatcatgtta atcgtttctt
     8581 gaacaatctt tcttcctaaa ccatctttac ggttgtcctg atgaacaagg acacgtccaa
     8641 aatggatact ctccgcttct tcatatactc gcgtgtaggc aaggatcttc ccatcagtat
     8701 cttgaaaaaa cgtatgccaa gcttctccat caatgtcatc aacttcttga taaggacact
     8761 gttgttccac aacaaaaact gcgattcgtt ctttgacgat cgcgtaaaac tctgctgttg
     8821 acaattcttc aaatttcttt acctgatatt ccactaagca aacgccccct gcttttttat
     8881 tcttagcata ccgattcgca cacaaaaaag caaacgaacc ttacgaatct caggctcgtt
     8941 tgctttttaa actgcttcag ttaaaaattc gatctcaact gtccgagtaa gtacatctga
     9001 atagctgtaa gatactcgtt caaaagaatt ttcttcagga tccaaatcaa caacgaatac
     9061 tgaaggatag gtttctgtta aaacgccgcg gcgttctgtt tgacgctttc ttcctgtctg
     9121 agcaaccaac atgattgggc tgccgatacg accttctaaa tctttcttga ttgaagctaa
     9181 agttgttggc atacagttca cctctactat caactatacc acatattttt aaaattttca
     9241 agtttaccat aaaaaaaaaa agaaatgcaa taaaaat
//
LOCUS       DAPCMT010000108         9224 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPCMT010000108 DAPCMT010000000
VERSION     DAPCMT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.110"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(91..1026)
                     /gene="murB"
                     /locus_tag="RD743_002147"
     CDS             complement(91..1026)
                     /gene="murB"
                     /locus_tag="RD743_002147"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDU0563950.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1099..2019)
                     /locus_tag="RD743_002148"
     CDS             complement(1099..2019)
                     /locus_tag="RD743_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0563951.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2132..2884
                     /gene="xth"
                     /locus_tag="RD743_002149"
     CDS             2132..2884
                     /gene="xth"
                     /locus_tag="RD743_002149"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDU0563952.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3748..4281
                     /locus_tag="RD743_002150"
     CDS             3748..4281
                     /locus_tag="RD743_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDU0563953.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4771..5295)
                     /locus_tag="RD743_002151"
     CDS             complement(4771..5295)
                     /locus_tag="RD743_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0563954.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5288..5761)
                     /gene="tsaE"
                     /locus_tag="RD743_002152"
     CDS             complement(5288..5761)
                     /gene="tsaE"
                     /locus_tag="RD743_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDU0563955.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5884..6864)
                     /gene="pta"
                     /locus_tag="RD743_002153"
     CDS             complement(5884..6864)
                     /gene="pta"
                     /locus_tag="RD743_002153"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDU0563956.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7017..7697)
                     /locus_tag="RD743_002154"
     CDS             complement(7017..7697)
                     /locus_tag="RD743_002154"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDU0563957.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7823..8692
                     /locus_tag="RD743_002155"
     CDS             7823..8692
                     /locus_tag="RD743_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0563958.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8718..>9224
                     /locus_tag="RD743_002156"
     CDS             8718..>9224
                     /locus_tag="RD743_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563959.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLN"
BASE COUNT         2667 a         1895 c         1675 g         2987 t
ORIGIN      
        1 caagatttgc gccatttgcg taaacaacca caaaaaagag caggacggcc accgtcctgc
       61 tcttttactt attttaagtt ccattactta ttaattggct aaacttctgc ttgtttctga
      121 tattgcttgt ttttctccaa tgatccgcac ttctgtttct aaagacacat caaatttttc
      181 tttgattact tcttggatgt gcgcaatcaa ttcaatataa tctgtcgctg tcgcatgatc
      241 gatattgacg atgaatccag catgtttttc agagacttgt gcaccgcccc atttcaggcc
      301 ttgcaatcct gcttgttgga tcagctgacc tgtatagtgt cctactgggc gcttaaagac
      361 acttccgcat gaagggtatt ctaatggctg ttttgattga cgcaattcgg tcaactcatc
      421 cattcttgct ttgattgctt cataatcccc tgtttgcaaa ctgaatcgag cttccaatac
      481 gatagcacgt aattcttgga ctttactgtg acgataagaa aataccattt cgtcttttgt
      541 caaagttttc aatgttccat cttcaagcaa aagatccact tcagcaaaaa catctttgat
      601 ctctccgcca tacgcacctg cattcatata aaccgctcca ccgacacttc ccgggatgcc
      661 gcaagcaaat tcaaatccag tcaatgattc tgccaaagct gcataagtcg tatcaatcag
      721 tgaggcacct gcttccgcaa tgaccatcgt tcctttgaca tgaatctgtt tcatttcagt
      781 cagcatgatg actacaccgc gaatcccgcc atcttgaacg atcagattgc ttgcatttcc
      841 taataccatc caaggtatat catggattcg gcaataatcg atgatttgct ttacttcttc
      901 ttgttttttc gggaatgcaa ggacatcagc tggtccacct gttttcgtaa acgtatagtt
      961 catcaatggt tcatcaaaca atatagtaat cgtggctaag ctttcaacta tttcttcttt
     1021 attcactgag gattttcctt tcttttctca aacgtcctca ttattctatc acgcttaata
     1081 caatcttgcc agtcttgttc aaagttgatg acggtagtga tctttaagca cttcctcaca
     1141 taattcatga cttcttacaa cttctttttg tttaagcaat tctgtttccg atttacctaa
     1201 acggatctga gaaaagaagg cctctaccat tggctcaaac ccgcgtttga ctagcgtgtt
     1261 tgtccagtcg ccaaattctt tcactttttt tccatcagcc tcttgaatga caagctgggt
     1321 cagatcgttt acttcataag taccattttt acttgttact tgatattttt ctatattagc
     1381 gccacttgct aaatccatcg ttaaaatagc cgtttgattt tgtgtttcta attgtaaaat
     1441 ggcatattcc aaaaattcct ttgtttctct tatctggtat tttgttctca aaatgggttc
     1501 atctaataaa tacacagctg tatcaataag atgtaaaaac aaatcataaa ggacgaactc
     1561 tgtcgtttct tttgccgcta ttcgattttt ttgcaggtgg atcaaccgct tatctgggat
     1621 acgtttcaat tcttctacca taggagcaaa gcgccggttg aaaccgatca tcaaaattat
     1681 ttggttctta tcagctaatt cttgcaattc acgaacttca tttagatgga cactcagtgg
     1741 tttgtcaata taaacatgga taccattttc tagacatttt ttggctaatt cataatgtac
     1801 ttttgttgct gcgtggatca tacatgcttc gattttttct tctaaaagct catcgatcgt
     1861 ttcatataga tgttggaacc cgtactcttt ttgaattctt tcttttgttt ctttatttct
     1921 tgttgcaaaa taaaaatcaa gtgttccttg ttttttcgca taagttggta aatatgcttt
     1981 ttctgcgata ttccctacac cgattacacc aatcttcatc tactttcctc ctggatcatt
     2041 tagttacttt catcttacac cattttttac taaaatgagg tacaatagta tcgcaaaggt
     2101 aaggaaatta attcgaagga gtgtgctttt attgaaattt atctcttgga acgtaaacgg
     2161 gcttcgtgcc atcgtaaata aaaatttttt agaagttttt catgagttag acgccgattt
     2221 tttctgtctg caggaaacga aactccaagc aggacaaatt gatttagatc ttccaggtta
     2281 ttatcaatat tggaattacg cggaacgaaa aggctattcc ggcacagcga tttttgctaa
     2341 aaaaccagca ttgaatgcca cttacggaat gggtatcgat atccatgata cagaaggccg
     2401 tctgattact ttggaatatt ccgatttctt tcttgtaacc tgttacacgc caaattctca
     2461 aagcgaatta aaacgtttag attaccgatt agaatgggaa gaagcctttt ataattattt
     2521 ggaaaatcta aaaaaacaaa aaccagtcat cgtgtgtggg gatctaaatg tcgctcatca
     2581 aaagattgat ttgaaaaact ggaaaacgaa tcaaaaaaat gctggattca ctccagaaga
     2641 aagagcggct ctttctcgac tgttggataa cgggtttatc gatacattcc gttattttta
     2701 tccaacccag gaaggtgtct actcttggtg gaactaccga ttcaattcaa ggaaaaacaa
     2761 tgctggttgg cggatcgatt actttttgac gagcaaagat ttagagccag gactagctga
     2821 tgcaaagatc catacggaca ttatgggcag tgatcattgt ccagtagaat tagatttgaa
     2881 ataaagaggt tgtgaaaaag tcgtttagtt tcgagtatta agaaaaattt tgtgaaaatg
     2941 gctttccaca ttttgcataa atttggcata ataccgagaa actggctttt gaacactgtt
     3001 tatcgagaga ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
     3061 agttcctcat atttttcgtt tggttgggct tattaccgca gatacaagct tttgaacacc
     3121 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3181 aatagttcct catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
     3241 accgtttatt aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac
     3301 aaaaatagtt cctcatattt ttcgtttggt tggacttatt accgcagatg caagcttttg
     3361 aacaccgttt attgagaagt tgtgaaatca gcgtcctgac ctgagtatta gagaaacaaa
     3421 tggcaaaata gttcctcata ttttgctatt ttttgagcta ataccgaggg tcagctgatt
     3481 gaacaccgtt tagtggagaa gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt
     3541 ttttgaaaat ggttcctcac aactttgcat aaatttgact taataccgag aagtcatgat
     3601 ccccatctaa ctccaaatca caaggaatga gttcaaaaac tagcttttct caacaaatat
     3661 tacataaaaa attctatgtt ttttgttaaa tttatttgct tttttcaaaa gtatgttaca
     3721 gtaatttccc aaggaggttt tacagaaatg aattttgttg ccatggattt tgaaactgcc
     3781 aatcatcagc cttatagtgc ttgttctctt gcgctagtca tggtaaaaaa tagccagatc
     3841 gttgatgaat tctatacatt gatacagcct gagactcctt ttttctggcg aaatgtccaa
     3901 attcacggaa tccatcaaga agacgtacgc aatgcgccga aattcccaga agtctggcaa
     3961 actatccaac catattttca aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat
     4021 acaaaggtct tagcgggatg cttagattat tatcatcttg aacagcctaa ttatctttct
     4081 ttatgtacag tgaaaagtag ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac
     4141 actgtgtgcg aaaatttgaa catccaatta gatcatcacc atgatgcatt ggaagatagt
     4201 cgtgcatgtg cggaaatcct gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa
     4261 aaacttgtga ctataaagta aaagaatact tatgaaacac acaaagaggt cgtgagcctg
     4321 ccgtttcact ccgagtcaca aggagcaatt tcaaaaaata gcttctcatg tttattaaat
     4381 attgtgactg gtgaccgagg agctggcagg agaacaccgt ttattggaga ggttgtgaaa
     4441 aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttca
     4501 tttggttgga cttattaccg cagatgcaag cttttggaca ccgtttacca agaggttgtg
     4561 aaatcagcgt tttgacctga gtattagaga aacaaacggc aaaatagttc ctcatatttt
     4621 gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttatt caaaaaaaat
     4681 ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt tttctgcctt ctcaaaagga
     4741 tgacagtttc tcactctttt cctatttctt ctattcaatc aaataactca ttttatatac
     4801 atctaaaaat tcacctagat ctgttcttgc ccctctaggc atgaccgctt cgatttggaa
     4861 gcccattttt cggtaaaggt gtacggctct ttcattacga acttgaacgt caagctccaa
     4921 tcgaaaaagt acacccattt ccttcgccca actgatgatt tcttctagca taagagtgcc
     4981 tagtcccatt ccccaatatt cctgcagaat actgataccg acttctccca cgtgagacaa
     5041 acgaaattga ctgtctgcct tgacagatgc tgttccaata atcgtccctt catagattgc
     5101 aagcagcaat aaattattat tgctttcgta tatataatcc aactcttctt ctaaggtttc
     5161 tggtggtaac aggagttctg cttcatccaa aactaaaaat tctgtctcct tattaacttg
     5221 tcccatcatc aaaagaatgt cttttgcatc tcctggcact gcttcccgca agatgatccc
     5281 aacttcttca ctcatgattt tcagcccatt tttctagaat tcgctgtttg aaggtttcac
     5341 tttgctcccc gtatgagatt agtttcacat accgcttttc taaatctgta tcactttttt
     5401 ctaaaatcag ttccaaataa tcctccggca gtgcttcttc taatagattt ccccattcaa
     5461 caacggaaag cccatcacct tcaaaatatt catctaaccc taaatctgct ccgctagctg
     5521 ctacccgata aatatccata tgatacaacg gaatccgacc ttgactatat tctctaataa
     5581 tcgtgtatgt cggacttttg atcatttgcg ttatccctaa tccttgggca attcctttcg
     5641 tcaacgttgt tttacccgca cctagatcac cagtcaatat cagattgtca cctggcattg
     5701 cggaatgacc aattgcctct gcgaattgtt ctgtcattgt taatcctgat agttcgatca
     5761 tttttcatct tctcccgctc ttctttcttt ttgccagtat atcgcgtaat gaagtttttt
     5821 taaagaaaaa atgaactagt caagaacagc agctgctgtt tttggctagt tcgcgtgtat
     5881 ttgttaatca atcaatgttt gtgctgctgt gataatcgat aatttatata catcttcttc
     5941 atttgctcca cgagacaaat cagaaaccgg tttgttcaag ccttgtagaa ttggtccgat
     6001 tgcttcaaag ttaccgaaac gctgagcaat tttatagccg atatttcctg actgcaattc
     6061 tggaaataca aagactgttg cttgaccagc tacttctgaa tctggcgctt tcagttgtgc
     6121 aactgaagga acataagaag catcaaattg caattcgccg tcgatcatgt attgtggcgc
     6181 tagtttttta gcgattttag ttgcttcaac tactttgtct acttctggcg atttcgctga
     6241 gccttttgtc gagaagctca tcattgctac ttttggttcg atatcgaaca attctgctgt
     6301 ttttgcagaa tctacggcga tttcagccaa ttcttgcgca gttggattca cgttgatcgc
     6361 acaatccgaa aagacatatt tttcttgatc tcttccacgt accatgatca tcgctccgct
     6421 tgtccgactg atgccaggct ttgttttgat gatctgaagt gctgggcgaa cagtgtcgcc
     6481 agtcgaatgg atcgctccag agaccattcc gtctgcaatc cccatatacg taagcatcgt
     6541 accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt tctttggatg cctttccatt
     6601 tctacgttca acaaaagtat ccaccatttc atcccactta tcgtaattat cagggctgat
     6661 gatcgtaaag ctagatgttt tgattcctct ggcatgcgct gcatttctta tttcttcttc
     6721 gttccctact aaaatgggtt ccacaagttc ttctgctttc aaccgggcag ctgcgcctaa
     6781 aatacgagca tcagtagctt ccgggaaaac gattttgatc ccacggcgaa caattttaaa
     6841 tcttaaacta tcaaacaatt ccacaagatg acctccagac gtttttatat gtattatcat
     6901 acactattcc agaaaaaaag aacagtttat tttatctgta tcagtcaaaa atgctggaaa
     6961 aattaagttt ttcacataca aagcgaactg cactataaca ggaaagcgct gttttattac
     7021 actgtttcag gcagttgcca atcaatcggt tcttctccta acgcaaggag cgcatcattc
     7081 gttttcgaga agggtttgga accaaaaaag ccacgacttg cagataacgg actgggatgt
     7141 ggagatgtta tgatgatatg gcgactctta tcgatcattt tgattttttc ttgtgccggt
     7201 tttccccaca agataaacac gattggtttt tcccgttcat ttaatttttc aatgatcgtg
     7261 tctgttaatc tttcccaacc tttcccgcga tgagaatacg cttgcccttc ccgaacggta
     7321 agaacggtgt tgagtaaaag gactccctgt ttagcccatg acactagatt tccatgttga
     7381 acaggtgcga ttccaagatc atcataaagc tctttataga tatttcttaa cgatggcggg
     7441 atctttacgc caggttgcac agaaaaagat aagccatgag cttgattttc tccatgataa
     7501 ggatcttgtc ctaaaatcac cactttaact tcttcataag gagtcaattc caatgcttca
     7561 tagatatgat acatatctgg atggatcttt tgggacgcat attctttttt taaaaattct
     7621 cttagatgca gataataatc ttttgaaaat tctgaggaca aaacttcttg ccaactgtta
     7681 tgaatgattt ctttcaaaaa ctccacctcc taataaaaag aataactcac attttcttca
     7741 atcacaaggg acaatcttca ccctcagcac ttgattaata tggtaaacta ggaacaagaa
     7801 gcagaaagga acgataatca ttatgatcaa attaattgca tcagatatgg acggcacatt
     7861 attagatgcc catatgagtg tttctcctga aaatgccgag gcgatacgtt ttgcaaatga
     7921 tgccggtgtt gagttcatgg tcgctactgg tcgaaactat caagaagctc gcgcagcttt
     7981 agatgaggta ggaatcgatt gtgcgatgat cactttgaac ggggcacagg tatttgataa
     8041 agaaggaaat tcattgttta ccgtgtcgat tccaaataat caagcaatct ctgttttaga
     8101 tattctagat gctaacggta tttactatga agtagcaacc aacgatggtc tttattctga
     8161 aaatcaagcg aaacgaattg aaagttttgc ttccatggtg gctacccatt tacctcacct
     8221 tacgtataaa atggcgattg ccatggcctc agctaagctg gagttattgc acatcacata
     8281 cgtggacagt attcgtgcta ttttggaaga aaagaatctg gaagttttaa aaattatttg
     8341 ttttcataca gaagggccag ctgtattagg tccagttgga aagcagattt ctcttttagg
     8401 cgatctagcc gttacttcct ctggtcaaaa caatatcgaa gtgaaccata aaaacgctca
     8461 aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg atttctttag aagaagtcat
     8521 gacgattggc gataacttca atgatgtcag catgctgcaa gtagcaggag tcagtttcgc
     8581 aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa tatacaacgg acacaaacct
     8641 tgaatctggc gtaggaaaag ccatattgcg cgcgattgca gaagacttgt agttttatct
     8701 agaaaagagg gatatagatg tcagaatttt tcattcagga aaagcagctt ggacgtgcaa
     8761 ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt tttgatggtt ggtcgctggg
     8821 gaacaaaggg cgatgcattg tctctttatg ccatgaatgg cgatctcgtt gctcacatca
     8881 agcagataag cctgacattc ggtacgcgtt ttgagctgta taaagagttc gaaaaggtgg
     8941 gaaccatgca gaaaatattc aattggcccg gtgactttta ttatattcgt agacttcact
     9001 ggacagtcca aggtgatatc tataatcatc aatatcaaat ccatcatttc aatcagcaaa
     9061 tcatgaaaat ggataaagca acactttttt caggagatta ctatgtattg gatgtctcaa
     9121 atgatgaaga tgctccagtt tgtatttgta ttgctgcggt tttagattac tggctctacc
     9181 gtaaagacaa aagtcgcgat aagcctgttt cttaccgact taac
//
LOCUS       DAPCMT010000109         9107 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPCMT010000109 DAPCMT010000000
VERSION     DAPCMT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.111"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(28..852)
                     /locus_tag="RD743_002157"
     CDS             complement(28..852)
                     /locus_tag="RD743_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDU0563960.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(825..1505)
                     /locus_tag="RD743_002158"
     CDS             complement(825..1505)
                     /locus_tag="RD743_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDU0563961.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1528..2466)
                     /locus_tag="RD743_002159"
     CDS             complement(1528..2466)
                     /locus_tag="RD743_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDU0563962.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2711..3574)
                     /locus_tag="RD743_002160"
     CDS             complement(2711..3574)
                     /locus_tag="RD743_002160"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDU0563963.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3716..6289)
                     /gene="mprF"
                     /locus_tag="RD743_002161"
     CDS             complement(3716..6289)
                     /gene="mprF"
                     /locus_tag="RD743_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDU0563964.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6430..7236
                     /gene="proC"
                     /locus_tag="RD743_002162"
     CDS             6430..7236
                     /gene="proC"
                     /locus_tag="RD743_002162"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDU0563965.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7315..8871)
                     /gene="rny"
                     /locus_tag="RD743_002163"
     CDS             complement(7315..8871)
                     /gene="rny"
                     /locus_tag="RD743_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDU0563966.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2683 a         2015 c         1568 g         2841 t
ORIGIN      
        1 ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg caaccaatat
       61 tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga ttgttgctcc
      121 tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta gaaatcctac
      181 acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac cgattgctgc
      241 gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca tcacagcaat
      301 agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta gcccatccac
      361 gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata aaaagaacaa
      421 tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg taatggtcac
      481 gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc tcaaattcat
      541 caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga tttctgaata
      601 ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa cgactagcat
      661 ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg ctaaagagac
      721 atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca cacctagcat
      781 cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga attcatatga
      841 aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc cgcatactgt
      901 ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac atgcgcacta
      961 tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt tgttggttca
     1021 tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat tcgctgcttt
     1081 tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc tacagcttct
     1141 aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca gcggcctctt
     1201 ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc attgaaagat
     1261 gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt tttctcagca
     1321 atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac caaggttgac
     1381 ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac atgataagag
     1441 acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat ttcgatataa
     1501 cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag ctttcagatt
     1561 tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag tcaagctttc
     1621 taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct cagctacctt
     1681 cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca caaattcttt
     1741 tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg aaatcgctac
     1801 ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag tgacaaatgt
     1861 gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt caagcttttt
     1921 gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt tttcgcctaa
     1981 ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc aaacatgtgg
     2041 atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt ctgcttcttg
     2101 cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt tgctatccac
     2161 tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca cgaaaacatc
     2221 tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg gctccgttcc
     2281 agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg taaaatcata
     2341 catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt ccttgttcgt
     2401 atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca aaagatattt
     2461 tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat taacgtgaac
     2521 taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg atttaagcat
     2581 gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag ccatttcact
     2641 acaataaaaa actgaagaat accattttag gattcctcag tttttcatca cctattttac
     2701 ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa ccttttaacc
     2761 cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg gttggtccgc
     2821 ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg atttgatgga
     2881 tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt ttgatcatct
     2941 gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga tgttggatgg
     3001 cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt ggtttgacca
     3061 acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct ttgccaccga
     3121 caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc aaaccgattt
     3181 ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct cttagagcag
     3241 ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc ttcttaacaa
     3301 agatcttgac accggttttg atattgtatt tattttttag caataaagca gcttgataaa
     3361 cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg attttgtctt
     3421 ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc accatttcca
     3481 actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt atcttggctg
     3541 gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc gcaagtattc
     3601 tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt ttgtcatcgt
     3661 ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt gattcttata
     3721 attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt cctatcagac
     3781 tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca aaataacgtc
     3841 cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag gaatagaaaa
     3901 aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa aaatctgttt
     3961 ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc attttctcgt
     4021 atacaataaa cagattggct aacagatagt cagctaattc ctcagattct ttaggtaaga
     4081 cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg atgaagccaa
     4141 cgactttttg atctttacgg acaataccga caccgctaga taatacatat gatttatcaa
     4201 atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt gaaacttcat
     4261 ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg tatccttctt
     4321 tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct gttgctagat
     4381 caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga agcatcacta
     4441 cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct gcttgtttca
     4501 taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct cctaaaacaa
     4561 agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg tcttcttggt
     4621 aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg cctccgtatg
     4681 tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc gtagtatctt
     4741 cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa ccaataagcc
     4801 ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt attcgaccga
     4861 ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa aagccgaaca
     4921 agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga taaaattctt
     4981 ttctggataa ccacacagct aacagaatca agacatatac caatatcaac tgccaagata
     5041 acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta ggcagaaatg
     5101 cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag ccaaccaata
     5161 gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga aatagccgac
     5221 tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc gcaaaataga
     5281 cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga atgttatcta
     5341 aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc gtcataaaag
     5401 gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca tcttgccgaa
     5461 cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt ccacctggga
     5521 ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc gaaagcatcg
     5581 aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct cccgtccatt
     5641 gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg aagaacaatg
     5701 tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta ccgccgaaaa
     5761 gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca tcaataaatg
     5821 taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt gatacagtgg
     5881 ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga cttgctccta
     5941 ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca gcaagaagaa
     6001 actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc gtcacccaat
     6061 catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc aggatcgtca
     6121 tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca ttagcaatat
     6181 ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt tttaaaatca
     6241 gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca ttttcccatt
     6301 cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt aatttttcca
     6361 ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa tgctaaagga
     6421 gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc tattattgat
     6481 ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag tggacatttt
     6541 gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag taatcaggaa
     6601 gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat tgaaaaagta
     6661 ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc ttcaggaaaa
     6721 tcattaatag atttagaaac agcgcttgaa gatcctcagg cctccatcgt acgtgtgatg
     6781 ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa tgaagctgcc
     6841 acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag cgtctatcct
     6901 ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc cgcattcgtt
     6961 tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc caaacagaca
     7021 gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct ttcaagtgat
     7081 aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac tgttgcaggt
     7141 gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat caatacgacg
     7201 atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt tttcgtgcct
     7261 tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt tttattattt
     7321 agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc caggataatc
     7381 cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt ctaaatccga
     7441 gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga
     7501 ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac ggatgtagtt
     7561 ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg ctgcagctac
     7621 aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga tcgcgttgat
     7681 tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa cgtgagatcc
     7741 ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac gttttgctaa
     7801 ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa cctcgaccga
     7861 atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga tgatcttgat
     7921 tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg
     7981 aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac gcgctggatg
     8041 gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc ttcggattgg
     8101 atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat cgataccagt
     8161 cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac ctttcatttc
     8221 atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg
     8281 ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt tggcacgctg
     8341 ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag tcgatttcat
     8401 gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt ctttttgttg
     8461 atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct gttttgaact
     8521 aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt catctttacg
     8581 attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt ctaacttact
     8641 ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct tagcttctaa
     8701 caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga ttccagcagc
     8761 tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca taaaccctaa
     8821 accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca taaaatccac
     8881 ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa ttattctaaa
     8941 ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact ttattctaat
     9001 gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta cttattcatt
     9061 aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaac
//
LOCUS       DAPCMT010000110         8919 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPCMT010000110 DAPCMT010000000
VERSION     DAPCMT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.112"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..488
                     /locus_tag="RD743_002164"
     CDS             <1..488
                     /locus_tag="RD743_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDU0563967.1"
                     /translation="IFEKPQIQQLTNIVSPAVRGVFLKFLEKAESEKIPIQVVIPVEV
                     KTIPIDLVVFIRCLSIFISNAFDHRVADQSPITINLLEEKEGSRFSIANKANPTNLTL
                     SEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFFVEMYLASRKSKSA
                     Y"
     gene            810..1037
                     /locus_tag="RD743_002165"
     CDS             810..1037
                     /locus_tag="RD743_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDU0563968.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            1160..1486
                     /locus_tag="RD743_002166"
     CDS             1160..1486
                     /locus_tag="RD743_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDU0563969.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1554..2267
                     /locus_tag="RD743_002167"
     CDS             1554..2267
                     /locus_tag="RD743_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDU0563970.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2577..3428
                     /locus_tag="RD743_002168"
     CDS             2577..3428
                     /locus_tag="RD743_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0563971.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            3549..5414
                     /locus_tag="RD743_002169"
     CDS             3549..5414
                     /locus_tag="RD743_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0563972.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5462..6871
                     /locus_tag="RD743_002170"
     CDS             5462..6871
                     /locus_tag="RD743_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDU0563973.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            6979..7680
                     /locus_tag="RD743_002171"
     CDS             6979..7680
                     /locus_tag="RD743_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0563974.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            7839..8318
                     /locus_tag="RD743_002172"
                     /pseudo
     CDS             7839..8318
                     /locus_tag="RD743_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         3006 a         1471 c         1963 g         2479 t
ORIGIN      
        1 ctatttttga aaaaccgcaa atacaacagc taacaaatat tgtatcccca gctgtcaggg
       61 gtgtcttttt aaaatttcta gaaaaagcag aatcggagaa aatcccgatc caagtagtta
      121 ttcctgtcga agtgaaaaca atcccgattg atctggttgt ttttattcgc tgtttatcta
      181 tttttatttc taacgcattc gatcatcgtg tagccgatca gagtccgatt acgatcaatc
      241 tgctggaaga aaaagaggga tcacgtttta gtatagcgaa caaggcaaat ccaacgaatt
      301 tgacgctatc ggagatggtc aaaagaggaa aaacctctaa aaaaaatggc gggttaggtc
      361 tatacagtgc gaaaaaaatg ttggatgttt acgaaaatgc agaactaaaa atcgaatttt
      421 caaaacagaa tcactatttt tttgtagaaa tgtatctggc atctagaaag agcaaatcag
      481 cctactgact tactgtcaaa ggataagttt gaggaaagtc tacacggaga tagagacaac
      541 aacacagcat attcaaaaga aacagaagcg tgatgaccgt tgtcaccctt ctgtttcttt
      601 tgctatgtag ttaaacaaag tagttgacac ttccgctact tagtattagt acgaaagtat
      661 acttcatgat actaagtaga taagaagtga aaagcggaac ccacgatcgt caaaatgcta
      721 actgctttgc ttacaccaga taaagggaag agcaatatcg cttattttag gcgtggagaa
      781 gcaagcaaat ccctacgatc acaaaaaaaa tgctgaccca gcagttaaga gagttagaac
      841 aagatgaaag tatccatcga aaagcctata accaagtccc gcctaaagta gagtactcgc
      901 tcaccgaaga aggaaaatca ttaagagaga ttctagtagc gttgtctgtt tgggaagaag
      961 aacgtatcga aaaacaaaaa gcctcaaggg aagaagacac gctactgcat acacatggtg
     1021 gctacctaaa ttattgattt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     1081 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaagtg
     1141 attggaagaa ggcaagcaaa tgaatagtat ggaaaaagtc aaccagttta gagatgaacg
     1201 taattggcga caatttcata acgaaaaaga tttggctatc tctatttctt tggaagcatc
     1261 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     1321 acgaatcaaa gaagaactgg cagatgtatt gatctattca tacatgatgg cagacaacct
     1381 tcacctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     1441 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttgtagaaat gtgtcattcg
     1501 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     1561 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     1621 gactagcttt gattttcaat gaacagatca aggattattt tgtcagagag acaggagata
     1681 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     1741 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcaaatga
     1801 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     1861 cggttttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     1921 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     1981 tgctgtttac accgctagaa aatttagaga tgggtgccaa gatctatttg acagatttag
     2041 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     2101 tactgaatga agtcgaggga aaagaaattg tcacactaat tacttgtggc gacatggacg
     2161 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacatcgata aaagatgcga
     2221 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     2281 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     2341 tgaaaaaaag agaaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     2401 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     2461 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     2521 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     2581 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     2641 tcgtttgtat gggaaaagga ctagcttttc aaaaaaagat tggggacgag atttcgccag
     2701 aagcggtcca aaaagaattt gttttaagag attcttttgc taaaaaccaa ttccagcagc
     2761 tgatggctga tattccggca gaggagatcg aatgggtcaa acaagtagtc gaactggctg
     2821 aagataaact aaaagttgaa ttttcgccta atatttatct gacattgacc gatcatcttc
     2881 attatgcgat cacacgagca aaagaaaata tccaactgcc taatccatta ttatttgaaa
     2941 caaaaaaatt ttatccgaaa gaatatcaag cagcgaaaga ggctcttttg ttcgtgaaac
     3001 aaaagacggg tgtggaactt tctgaggatg aagccggttt tattgccttt cattttgtga
     3061 atagccaaca aggaaacgaa gacatgcaag tgacgatgac cgctaccaca atggtaagag
     3121 atatactgag tatcatcagt aaattcttcg gcatcgtgtt cgatgaagaa tctttgaatt
     3181 atcagcgtat cattactcat ctgcagtttt ttgcccagcg ttatctcaaa ggggaaggat
     3241 ctgacgagca agatgaattt ctctacgcat tagttcaagg aaagtatcca aaagcgtttc
     3301 attgtgtcga acggatcaat gagtatcttc gacaaacgca aaaacaggaa atggggatcg
     3361 cagagcagat ttatttaacg attcatattc aaagagtagt aagtgagaaa aaaagcatcc
     3421 aatcataagg attgttactg tatggcaggc aaaacctgga ttgaaaaaat aggttcacga
     3481 atgtttgtga ctgttttttt caatctgggt ttttattttt taaataaaaa agaaagaagg
     3541 aaaaaacaat ggataatcaa gcagttggtc gtcgtgtatg ggaagcggta ggaggacaaa
     3601 agaatgtcaa aagtttagta cattgtgcta cgcggttgcg attcagatta aaagatgaat
     3661 ctttagcaga tacgcaaaag ttaaaagaag acccggacgt gatccaagtc gtacaaagtg
     3721 gcggtcagta tcaagtagtg attggcagta atgtagcaga tgtttatcaa tcgatcgtgg
     3781 atgaggaagg tcttgcagtt tcggaggatg gaaaagaaga gtccaaaaat attttgaatc
     3841 gtctgatcga tattatctct agtatattta caccttttct aggggcgatg gcagccgccg
     3901 gggtattgaa aggattctta tcattggcaa cggtgatggg ctggctgaca gctgatagcg
     3961 gtacttatca aatattgttt gctgcagcag atggtgtctt taccttctta cctgtcatgc
     4021 ttgcttttac agcagctaaa aagttcaaag ccaaccagtt tttagcagta gcaattgcta
     4081 tggcacttgt gtatccagct atcacagcag ttgctggtgc gggagaagct atcagtttct
     4141 ttgggatccc agtgattctt tcaccatctg gttatacttc ttctgtcata ccaatcattc
     4201 tagccgtatg ggttcaaagt aaattagagc cattcgtaaa aaaagtgatt ccgcaatttc
     4261 tgcaaatgat tttagttccg cttgtcgttt tagtagtgat ggttccatta acatttcttg
     4321 ctttaggacc aatcgggacg gttgccggta atgctttagg cggtctcttc aactcgattt
     4381 atggatttag tccaatcgta gcaggtctaa tcatgggaag tttatggcaa gtattcgtga
     4441 tgttcggcat gcattgggga ttcgtaccga tcatgttttt gaatatcgaa caatatgggt
     4501 ttgatgtatt gatgccaatg cttcttccag ccatcttggc acaaggtggg gctgctttag
     4561 ctgtagcatt gcgaacaaaa gatacgaaac ttcgagcatt agggatttct tctacagtga
     4621 cttcgttatt tggaatcact gaaccaactg tgtatggtgt gacattacct ttgaagaaac
     4681 cattcattgc cgcttgtatt tctggaggta ttggtggagc tattatcgga ttttccggag
     4741 taaaagcttt ctccagcagt ttggtcagct tattgacgat tccgactttc attaatacgg
     4801 tggatggtgt agagtcaaat gtgacagtag cagtaatagc tacaggaatt gcttttgttc
     4861 ttgcattcgt aggtacattg attttaggat ttgacgaaca aacacaagac aatcaattag
     4921 aaaacaaaca tgcgaatgca ggaaaaccga ttacttcagc acggcatacc ttaaaaagtc
     4981 ctttaacggg taaagtactg ccattgagtg aagtaccaga ccaagttttt tcttctggcg
     5041 tgatgggaaa aggtatcgct attgatccag aagtaggaga actagttgca cctgcagacg
     5101 gagagatcac gactattttt ccaactggac atgctgtagg gatcacaacg actgacggag
     5161 cggagatttt gattcatatc gggatggata cagtagaatt aaatgggaat ggctttgaga
     5221 tcttagtcaa acaaggggat ctcgtcaaag caggagattt attgattcgt tttgacatcg
     5281 aagcaattag agcagctggc tatagcgtaa tcactccagt tgtcattacg aatactgatg
     5341 catttgccga tatacttgaa ttagaccaaa aagagattat tgcaaacgaa gatgttctgg
     5401 caattgtaaa ataaggagga aatcaggatg caatcacgtt tttcagaaaa atttttatgg
     5461 ggtggagcaa ctgctgccaa atcaattaga aggggcttat gatcgagacg gaaaaggact
     5521 atccgttgca gatgctatgc ctggaggtaa gcagcgcttt gcgattatcg gcagtgaaga
     5581 atttgactgg acgattgatc aagaaaaata tatttacccg aatcatcgag gaatcgatca
     5641 ttacgatcgt ttcaaagaag atcttgcctt gtttgcggaa atgggattca aatgttatcg
     5701 tttttcgatt gcctggacac gaatttttcc aaatggagac gaaggtactc ctaacgaagc
     5761 gggattagaa ttttatgatc agctgattga tgagtgctta aaatatgata ttgaacctgt
     5821 gatcacgatt tctcattacg aaatgcctct tcatttggca aaagaatacg gtggctggaa
     5881 aaatcgaaaa ctgatcgact tctatgaacg atttgctcag acagtattag agagatacag
     5941 cagtaaagtc aaatattgga tgactttcaa tgaaatcaat tcggcctttc actttccagc
     6001 tttaagtcaa ggattagtaa aaagcaatgg agcaggagag taccaaaata ttttccaagc
     6061 atggcataat caattcgttg caagtagcaa agccgtaaaa atcggccacg aattgcgttc
     6121 ggatatccaa atcggctgca tgattatcta tgcaacaact tacagcatcg atgccaatcc
     6181 agtcaatcag gcagcaacaa tgatccaaaa ccaagaattc aattttttct gcacggatgt
     6241 ccaagtacgt ggagaatacc ctgcatacac agcgcgtacc cataaaaaat atggagtcga
     6301 tccggaaaaa ttagagcaaa cggaaaaaga cttccagttg ctaaaagaat atccagtgga
     6361 ctacatcgga tttagttatt atatgtctac ggcaatcaat gagactgatc cacaagcagc
     6421 aacttcagaa gggaacttgc tgggaggagt aaaaaatccg tttcttgagg caagtgaatg
     6481 gggctggcag atcgatccag aagggctgag aatcgcattg aatgaattat acaatcgtta
     6541 ccagaaacca ttattcattg ttgaaaacgg tcttggagcc attgatcata tggaagagga
     6601 tggcacgatt attgatgatt atcggatcga ttatttacgt cgtcatatcg aagcgatggc
     6661 taatgcagta gcagacggtg tagatttgat gggctatacg ccttggggat gtatcgatct
     6721 tgtcagtgca tcaacaggag aaatgagtaa acgttacggc tttatctatg tggatctaga
     6781 cgatgaagga aatgggacac tgaatcgatc aaagaaaaaa tcttttgatt ggtataaaga
     6841 agtaattgcc tcaaatggcg taaacttgta attaaagcag ctgaatgaaa aagaacacac
     6901 ttctcacatg tgatttgtgt tcttttttta ttttttatgt gctattcttt tagtaatagt
     6961 aaagcaaagg gtgacgttat gaaaaggaca cgtgtactat acttggaagt cgcagataaa
     7021 attaaagaag atattttctc aggaaaatat ccagtcggtt cgatgttgcc gacagaaagt
     7081 gaattagaag aactgtttca agtcagcaaa atcactgtcc gaaaggcaat cgaactgctg
     7141 gcatcagatg aatatgtcgc aaagaaaagt ggtcgtggga cgacggttct aagcaatcgt
     7201 ccttacaatc gattatccaa agcggcaagc tttacgcaaa tcttggagaa ctcccgatat
     7261 gaagtccgga aagaaacgct ggatttgcaa ttggtccata taaagaagga agatcctttg
     7321 ttttcctttt tcggtgagca agctgtttgt tttaagcgct tatattattt gaacaatcag
     7381 ccttatattt attttgttca ttatcttcca caagaaatga tgcaattcag cagagaagaa
     7441 tttggcaaag aatctttgta tcgtttgctg acgaaaaaag gatatactat tgactcattt
     7501 tctgatgatt tctcggcagt atttttaaca gaagaagaac aaggaattct gaaaacagac
     7561 gcacagatcg gcatcaaacg gatacggaaa tcgtctacgc aaattgggga aatgattgaa
     7621 tattcggagg cggtctacca tacggagctg catccttacc atattgatta tgaaacataa
     7681 ataaaaaaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     7741 aatagcttct catatttttc gtttggttag gcttattacc gcagatacac ggctgtttct
     7801 ggattaccat ttattctgga taagggggtg tggcacgatt ttcagaagga atctatgaac
     7861 caatcactgt attgatttta gcttcgatgg taaccccatt cggcttgacg atcgcttatt
     7921 tcttagggaa aatcattcgt aaaaatatac tcaatcgtca agaaatcgat acattgaaga
     7981 ccgcttttcc tatggggatc tgtcagatca cagagggatg tttccctatc gtattgaacg
     8041 acttggcacg caatgtcata gcaacgggag tcggtgggac agtaggtggc ggattaagca
     8101 tgttttgggg cgcagatagc cgtattccgg catcgggaat gtttgcagtc gctacgatga
     8161 ccagaccatg ggcattcatt ggtgcattgt tagctggttc ttttgtcaca ggaataacca
     8221 ttttattatt gaaaaaacca gtagatccga atgcagaaat catccaagaa gaaaaagagg
     8281 aagaagacat atcttgggat gatttgacaa ttagttagga gaggcagaat ggattacaag
     8341 aaaaaagtac aatagatgta ccttgagaca ggaatggtat gaaagaatca gagttagaaa
     8401 gaatcgaata tacagatttt ggtttaggag cgatcgaaac tgtccagagc atcggcaccc
     8461 tccttgaaat gacgatgaag gaaaaatgag aacctttcgt tgtcgaaagg gtctggtata
     8521 ccttatgtcg aaggaaagga aacaaaaaaa ctttcctgta ctattccaaa aaggaaagaa
     8581 agttattata cagtaaaaaa agaaattatt cttcatgcta gagatcagta cacgttcgag
     8641 cccaatatca aacactagtt ccaggcaggc gcaacaggtg cggttatcac tgaattttct
     8701 tccagcagtg acgattcaag tgatattttt acaaatccag ctatttatcg ttaacaaaaa
     8761 gatttttagg agaagctgat gaacaaaaat gatcaaattt tgatcgttta gttcgtcggc
     8821 ttttttattt agagaaacat catttatcag tgaaacataa cacgagaaca aaatgatttt
     8881 ttcgttaata aaagaaacaa gtatttgttg tttaaaata
//
LOCUS       DAPCMT010000111         8851 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPCMT010000111 DAPCMT010000000
VERSION     DAPCMT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.113"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            63..1277
                     /locus_tag="RD743_002173"
                     /pseudo
     CDS             63..1277
                     /locus_tag="RD743_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1785)
                     /locus_tag="RD743_002174"
     CDS             complement(1456..1785)
                     /locus_tag="RD743_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDU0563975.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="RD743_002175"
     CDS             complement(1775..2047)
                     /locus_tag="RD743_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDU0563976.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="RD743_002176"
     CDS             complement(2498..3301)
                     /locus_tag="RD743_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDU0563977.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="RD743_002177"
     CDS             complement(3279..4403)
                     /locus_tag="RD743_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0563978.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="RD743_002178"
     CDS             complement(4419..5234)
                     /locus_tag="RD743_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0563979.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6096)
                     /locus_tag="RD743_002179"
     CDS             complement(5224..6096)
                     /locus_tag="RD743_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0563980.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="RD743_002180"
     CDS             complement(6181..7470)
                     /locus_tag="RD743_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0563981.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="RD743_002181"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="RD743_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2742 a         1883 c         1410 g         2816 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca g
//
LOCUS       DAPCMT010000112         8829 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPCMT010000112 DAPCMT010000000
VERSION     DAPCMT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.114"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(37..216)
                     /locus_tag="RD743_002182"
     CDS             complement(37..216)
                     /locus_tag="RD743_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563982.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="RD743_002183"
     CDS             complement(597..1304)
                     /locus_tag="RD743_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563983.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="RD743_002184"
     CDS             complement(1297..1734)
                     /locus_tag="RD743_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563984.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="RD743_002185"
     CDS             complement(1749..2000)
                     /locus_tag="RD743_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563985.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="RD743_002186"
     CDS             complement(2000..2206)
                     /locus_tag="RD743_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDU0563986.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="RD743_002187"
     CDS             complement(2643..2903)
                     /locus_tag="RD743_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563987.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="RD743_002188"
     CDS             2978..3181
                     /locus_tag="RD743_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDU0563988.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="RD743_002189"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="RD743_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDU0563989.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="RD743_002190"
     CDS             complement(3810..4709)
                     /locus_tag="RD743_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDU0563990.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="RD743_002191"
     CDS             complement(4722..5315)
                     /locus_tag="RD743_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDU0563991.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="RD743_002192"
     CDS             complement(5391..5540)
                     /locus_tag="RD743_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0563992.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="RD743_002193"
     CDS             complement(5556..7760)
                     /locus_tag="RD743_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDU0563993.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..8793)
                     /locus_tag="RD743_002194"
     CDS             complement(7771..8793)
                     /locus_tag="RD743_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDU0563994.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQG
                     RQQERE"
BASE COUNT         2253 a         1823 c         1507 g         3246 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctgg
//
LOCUS       DAPCMT010000113         8740 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPCMT010000113 DAPCMT010000000
VERSION     DAPCMT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.115"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            38..1099
                     /locus_tag="RD743_002195"
     CDS             38..1099
                     /locus_tag="RD743_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDU0563995.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1102..1998
                     /gene="murQ"
                     /locus_tag="RD743_002196"
     CDS             1102..1998
                     /gene="murQ"
                     /locus_tag="RD743_002196"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDU0563996.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2012..3994
                     /locus_tag="RD743_002197"
     CDS             2012..3994
                     /locus_tag="RD743_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0563997.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4059..4505)
                     /gene="nrdI"
                     /locus_tag="RD743_002198"
     CDS             complement(4059..4505)
                     /gene="nrdI"
                     /locus_tag="RD743_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDU0563998.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4716..6140
                     /locus_tag="RD743_002199"
     CDS             4716..6140
                     /locus_tag="RD743_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0563999.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6321..7094)
                     /locus_tag="RD743_002200"
     CDS             complement(6321..7094)
                     /locus_tag="RD743_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDU0564000.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7091..7996)
                     /locus_tag="RD743_002201"
     CDS             complement(7091..7996)
                     /locus_tag="RD743_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0564001.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8150..8470)
                     /locus_tag="RD743_002202"
     CDS             complement(8150..8470)
                     /locus_tag="RD743_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDU0564002.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2743 a         1572 c         1868 g         2557 t
ORIGIN      
        1 atagaaatga agacggatac agaaaaagag ggataatatg tttggctttt caatatttat
       61 gaacgattct ttgtcagaag agaagaaaca gtatatccgc caaatggcat ccaatggctt
      121 tgtcggtatt ttcacttcca tgcatatacc agaagatgat gtttcggcat atcaaaagcg
      181 attgatcgaa ctaggaagcg aagcgaaaaa gaataaacta gaattgatgg tcgatatttc
      241 aggagaggca ttggaacgag cgggtttttc attagaagat ctaacgcctt tgaaagcaat
      301 tggtgtaaca ggtttaagaa tggattatca tatttccaat cagcagattg ccgactggtc
      361 acatcaattg aaaatcagtt tgaatgctag cacgatcacg cctaaagata tcgatgaatt
      421 gaaagaagca gaagctgatt tctcacagat tgaagcttgg cataattatt atccacgtcc
      481 cgaaacaggg ctggacaaag aatggtatca gaaaaaaaat caatggctca aaattcaagg
      541 tctccttgtc caaggttttg taccaggaga tacagagttg agaggcccac tatatcaagg
      601 attgccaact ttggaagaac atcgagggat ccatcctctt gcaggcgctc tagatctatt
      661 agcaagtaac acggatattg tttacatcgg agatgcaggt ctgtcagaaa acgttcagga
      721 gcaatttgct tctttccaaa aagaacagac agtcttgctc catacggaac ctgtagatga
      781 agaattttac gaatatatat tagggaagca tacgaatcgg caagacgatg caagagatgt
      841 catacgaagc gtagatgcac gttttcgaga gattccgcct atcccagcaa gaaatacagc
      901 tacgcgtatg aaagggtcta ttactttaga taacgaaaag tatttgcgct atatgggtga
      961 gatccaatta accaaatatg atttgccagc ggatgaaaaa gtgaatgtag tagctaaagt
     1021 tatcaaagaa gagctaccat tgatcaacca aatcaaagct ggtatgaact atcaatttat
     1081 aagaaaagaa ggaagataaa aatgagcaaa atcaatttag agaacctaac aacggaacgc
     1141 agaaaccaag aaacttttgg attagatgag atgagtattt ctcaagccgt tcaactaatg
     1201 aacaaagaag atcagaaagt ggccgctgcg gttgaaaaag aactgacaag tatcgaacca
     1261 gtgattgcta acacgattga gtcctttaaa aaaggtggac gtctgattta tatgggagcc
     1321 ggaacaagtg gaagactagg tgtgttagat gctgctgaat gcgtaccgac attcggtgta
     1381 gaaccggaaa tggtcatagg tctaattgct ggtggagaag aagcaatgac tgtagctgtc
     1441 gaaggggccg aagattctta tgagctaggg caagaagatc taatcaagct gaatttgaca
     1501 aaaaatgata tagttattgg tatcgcagct agcggtcgta caccatatgt gatcggtgga
     1561 ttagattacg caaaatcgat cggtgctgtc acaggaacga tttcttgcaa taaacaagcc
     1621 gaaatcagta aacacgcgga tttcccaatc gaagtcgatg caggtccaga atttttgaca
     1681 ggctctactc gattgaagtc aggaacagca caaaaactta tcttgaatat gatttctact
     1741 atcagtatga ttggtatagg taaagtattc aataacttga tggtcgatgt caaaccaaca
     1801 aatgaaaaac taatcgaacg ctcaaaacga atcatcatgc aagcgacaga agctgattac
     1861 gaaacagctg aaaagtactt tgaagaagca gatcaaaatg ttaagctggc tatcgtcatg
     1921 attttgacaa atagtgataa agaggaagct caagcaaaat tagctgaagc aaacggattt
     1981 gtaaaaggga ccttatgagg gggaaaagac catggcaaaa aacgaactga ctttggaaga
     2041 actggcaaaa caaatttata aaggggcagg cggcatgggc aatgtagaaa gcattgtcca
     2101 ctgcatgact cgtgtgcgta tgaagatcat caatgatgat cttgtaaatg tctctgaatt
     2161 aaaagcgatt ccaggagtgc ttggtgtagt agaagatgaa cagcttcaag tcatcatcgg
     2221 acctgggaaa gtgaacaaag tagcacagca aatggttgat ttggcaggcg tgaaattagg
     2281 agaaaaactt tcttctgtaa gtacagctac agcaaattca tcagcatctg gaaaagaccg
     2341 tgtaaacgaa cgggcacaag aaatgaaagc ggcacaaaaa tcgaaacaaa aaccatctaa
     2401 aatcaaatcg gtattaaaag acatttctaa tatctttgtg ccaatgattc ctgcatttgt
     2461 cggtactggg atcgtggcag ggattgccgc agttttagca aacttagtca cagccggaac
     2521 acttgatgca gcaacatggc aacaatatat cgatgtgatg aacatcttga aaaacgggat
     2581 gtttagctac ctagttatct atacaggtat caatgctgca caagtttttg gtgcaacacc
     2641 aactcttggt ggagttatcg gggccgttgt gatgctgaca gggatgaatc cggaagcacc
     2701 actgaaaaac ttatttactg gtgaagctct tgcagctgga caaggtggga tcctaggtgt
     2761 catcttcgct gtttggctat tgtcaatcgt tgagaaaaaa ctgcataaag tagtacctga
     2821 tgcggtagat atcatcgtta caccaactct ttcattgatt gccatcggtc tgattgaaat
     2881 tttcttcatc atgcctttag caggtttcat ttctgatggc atggtcggtg ggatcaactg
     2941 ggtacttggc gttggtggag catttgccgg cttcgttcta ggcacattat tcttgccgat
     3001 ggttatgttc ggactgcatc aaattttgac tccaatccat gtgcaaatga ttgatgaaac
     3061 tggtcgtacg ctattgcttc caattcttgc gatggctggt gcaggacaag tgggtgcagc
     3121 attggcatta tggattcgct gcaaaaaaga taaagaatta acagaaatga tcaaaggagc
     3181 tttaccagtc gggatcctag gaatcggcga accattgatt tacggagtaa cattacctct
     3241 aggacgccca tttattactg cttgtatcgg tggaggtatc ggcggggcag tgatcggaat
     3301 gatcggaaat gtcggggcta tcgctattgg accttcagga gccgctttga tcccattgat
     3361 ctctgacggt aaatggttag gctacatcct tggattattg gcagcttatg ctggtggctt
     3421 cgttgctaca ttcttcttcg gtattcctaa agaccgttta gaaaaagaag aattagcgac
     3481 tgaaacagcc acagtaaatg aagtgcctga aaaagtagta cctcaaactt cagctgaaag
     3541 tgtgactatc ctctcatcag ttgctgacgg aacagttgtt tctttagata aagcaagtga
     3601 tccagttttt gcacaaaaga tgatgggtga aggctacttt gtagaaccag aaaacggaca
     3661 tatctattct ccagttactg gggtagtcag ctctgtcttc ccaacgaaac acgctattgg
     3721 gatcacaacg ccaaatggtt tagaaatctt gcttcatatg ggaatcaaca cagttgactt
     3781 aggcggtacg ccatttgatg tgaaagtaac agaagggcag caagtgacac ctgatacgct
     3841 tgtagcagaa gctgacttag aagcgatcaa agcagcaggc aaagaaacat ccatgatcgt
     3901 cttggtaacc aatatggatc gtgtaaaaaa ctttgtcttg gaaaaaacag gtaaagcaaa
     3961 agcaaaagca cctgtgatgg atgtcaaagc ttaatatgat agaaacaata gaaacgaaag
     4021 aagaaactga aatctatttt tcagcttctt cttttttatt atttattgtt ttctgctaaa
     4081 atctgataaa ttcgtgtcac atcagatggt gttccccgta attcataatc tgccaagaaa
     4141 ggaaagccaa atgtttgtgc atattgtttg gctgttagac aatactgatt attgaagttt
     4201 ttatttccgc taccgactac accaagacaa agtttatgat tgtcttcatg ttccagatat
     4261 tcacgcatcg tttcggtcaa gatttctgta tcaccgttat ctaaaccatt tccgccgtct
     4321 aaataagtag gaacgaaagt aaaaaatggt tctgtttctt tcgtaaaatc actattttca
     4381 tggatttctt ttaaagtaac ttcaggattg acaggatctt ctgcatgcat tttttcagca
     4441 tattctgcta gatgtttggc aaaggcacgc gtatttcctg aaatcgatat atataaaatg
     4501 ttcatggatc atcctccaat tctttccttt tagtatagca tcttttttaa gttttttggt
     4561 ggtgtaattt ggaaagggac taggcgcttt gcttgtataa tgagaaaaaa gccggtagaa
     4621 ttatatttct attaaagttt ttattaatta agtaaaaata aaaaaggttt gttatacttt
     4681 ttgataaaag cggttttatt ttggaggatg cttacatgga tacagagcaa cagaaaaaag
     4741 gaattggttt aattccactt gctgccttag taattggttc agcgattgga ggtggcgtat
     4801 ttggtattat gacggacatc tccggatcgg ctggcggccc agctattttc agttgggtac
     4861 tcgtaggaac agcaatgttg atgctctcct tgtcgatcaa taatatcaat aaaaaacgtc
     4921 cagatttaaa aggcgggatc tttagttacg cagaagaagg ttttggccgt ttcgctggtt
     4981 ttatcagcgg ttggggttac tggctcacat gctggttagg caacgtagct tttgcaacat
     5041 tgttgatgag cgcattagga tatttcttcc cagtatttga aggtgggcaa aatctggcat
     5101 cgatcgtgat gagtacagtc tttttatggg gatatgcatg gctagtgaat cggggagtcg
     5161 aaaatgctag tttcgtcaat gctatcgtca cattctgtaa attagtgcca ttatttttat
     5221 ttattgtagt agctatcagc acgttttcga tccaacaatt cattgataac ttttctagcg
     5281 acattatatt gaatgcgaat ggacagccga tcccgttttc cagtcaagta ttgagttgca
     5341 tcatggtgat gctttgggta tttgttggta ttgaaggagc ctctgtcgta ggttcacgag
     5401 caaaaaaaca atcagatgtt gggaaagcaa cgattctagg gatattcggc ttgattatta
     5461 tttacgtctt ggtttccatg cttccatatg ggacaatgac gatgaatcag ctgcaaacga
     5521 tcaaggcgca gcctgctatg ggctatgtgt ttgaaatgat ggttggtaaa tggggagcag
     5581 tcgtgatcaa cggcggtttg atcatttctt tagttggtgt ttggctctca tggacgatct
     5641 taccgattga aacaatggga aacatggctg aagacggact tttgcctaaa gcatggggaa
     5701 aactcaacaa aaatggggca ccaacttatg ctatcgtatt gacgacgtta tgtacgaatg
     5761 tctttttact ttcattatta ttcactgaca gtgcttataa ctttgcttac acacttggta
     5821 cagcagctat tttcttcacg tggcttttct taggattgta ccaaatgaaa ctttcttatc
     5881 agcggaaaga atgggtccaa tttttagtag ggcttttagc aagtgccttt caagtatggg
     5941 ctatgctagt cgtagccttt aatgaagtaa tgcttgttct tgttcttttc ttgccaggca
     6001 tttattttta cctcaaagca cgtaaggagc aaaacgagcc tgtacaacat atccacttag
     6061 ataagatttt acttataggt gtgacgatgg tcggagtgat tgcaatggtt ttatttgcca
     6121 ctgggatgct tcacgtatag aagtataaaa gaagtataaa gagaggttgt gagcctgtcg
     6181 tttattcaaa aacatactag gaaaggagct gtgacacaaa tgttgtcaca gctccttttt
     6241 cagaataaac ggtgtttaag agacagccaa tcggcaataa ggctgaacgt ctctttcaca
     6301 gcctcttttt tgatgagtca tcatacattt tttcgcttaa atacgagata tcctaatgct
     6361 aagaacaaaa cgatgtaagc taaattaccg aatagcattt gattagtaga aagatgagtc
     6421 aattgcttca tgatctcttc gtttccaatt tggttttgca gattcaacat actgatcgga
     6481 ttccatttga tccactccca tttttggatg agtgcaataa ggataccaga tatgattgac
     6541 gaagcaaaat aaaagacgat tcctgctgag atggatgcgc cggaactatt gataaagcaa
     6601 gcaatcatca acactaaact taaaatcagc cataaaccga tataactacc caaagtataa
     6661 gtgaacaagc tttggatcag tgtttgacca gtagaaacct tctccgtgaa ggaaatcgaa
     6721 gggaaaagaa tcagtttcat caacacagtg acaataatag ctaaaaggta taaataaaca
     6781 gaataaataa ctaatgtgat ccatttgctt actaatattt ctcctcgaga atactttcga
     6841 taaaggagat ttttcaatgt accatattgt gcttccatcg aaatgatcgt gcttgcagct
     6901 gcaatcatga taaagacaat ccaagatgtg gcactaaaaa gctgagcagt catttcaact
     6961 ggatcaatga tcgtcgtgta ttttttggct agaagtgctg ttcctatgag caaaacaact
     7021 aatagaacag ataaaacagc agttgacttt ttcttgatca atttaaataa ttcttgtttt
     7081 acaagcaatg tcatttttct tcttccccct ttactgaatc cagaatttcc aatagagaag
     7141 attccaaatc attcgtcaga tgctggacgt ctgtcacatg gatattttgt gcagacaata
     7201 gctggatcac ggttcctaat aattcttcgt tttcttttaa aagcttgatt ttatcaggat
     7261 cttccaaagt atatccagct tctgtcaaga tcgttcgagc agctagatca tttgttgtag
     7321 agataacgat gaaatcttgc gctgtttggt tcaattcagc cattgtacat tgtttgatga
     7381 tctttccttg attgatcagg atcagctcat cgaccatttt ttcaagctcg ctgagtaagt
     7441 gactggagat caagaaagag gtgccctgag tagccaagtc ggtaatgatt tttcgcacat
     7501 ctcttgttgc ttgtggatcc aaaccgttca tcggctcatc taaaataacg atagacggat
     7561 ggttcagtaa ggcaagagca attcccaatt tttgtttcat tcccaacgag taggatttcg
     7621 cttttttgtg aatgtacttt tccatcttga gctgattcac gatagtgtcg atagcagata
     7681 catcgttggt ttcagagaat aacttcagat gatcatagcc tgttaagaat ggatagatac
     7741 ccgggtattc gatcaatgct cctacttgtt ccaacccttt atgactagag ggagaaatct
     7801 cattttcacc aatacagatt gttccatctg agtaatggat cagtcctaaa atagctttca
     7861 tgatcgttgt ttttccagcg ccattaggac ctactaatcc aacgattttg ccaggggata
     7921 agctaaaact gacatcgctg agggcttgtt tgtgaccaaa gttcttggat aattcttgga
     7981 ccgaaagact tttcaaaaat attcactcct tttctataga tagtacaagc atacacctga
     8041 tgagtggaga agtcgctagg tcattggact tatttcttat tttttcataa aaaaattgaa
     8101 tattaaataa aaaggcttat tttttctgat cagtacttat tccaagtgtc tagtggccgc
     8161 tagtaatctt gatgccaatg atactgatga gtagcatccc cacaaagaac caagtggaaa
     8221 gattcagctt atcatgaaac aagaccacac caattatgac cgaaccaact gcgccaatcc
     8281 ctgtccagat aggataagag agactcatcg gcaaactttt gattgccttt gacaaaaagt
     8341 aaaaactagc aatcatgcca atcactgtgt aaaaagaata attgatttta gaaaaaccat
     8401 cactccattt catcatcgtt gaccagaata cttccaaaat tcctgctatg attaattcca
     8461 tccatgccat acttacattc ctccttaact ataaaaaaat acacaaaaag aggaaaatca
     8521 tcccttcaaa gacctaatgg aatgatcatc ctctttatcc tacccggtgg cagattgtat
     8581 atctttttct gtagtatagc acacctatcc agatatcggc aatctcatac tttttagtaa
     8641 agtgtaccaa gcactgacgg atatatcgtt agcggaaaag aagatgaatg ctttgcaatt
     8701 tcatagaaag aagttgttat ttattaatca tttaaaaaaa
//
LOCUS       DAPCMT010000114         8574 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPCMT010000114 DAPCMT010000000
VERSION     DAPCMT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.116"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="RD743_002203"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="RD743_002203"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDU0564003.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1592..2938)
                     /gene="gor"
                     /locus_tag="RD743_002204"
     CDS             complement(1592..2938)
                     /gene="gor"
                     /locus_tag="RD743_002204"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDU0564004.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3072..4325)
                     /locus_tag="RD743_002205"
     CDS             complement(3072..4325)
                     /locus_tag="RD743_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDU0564005.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4331..5254)
                     /locus_tag="RD743_002206"
     CDS             complement(4331..5254)
                     /locus_tag="RD743_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDU0564006.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5475..7967)
                     /locus_tag="RD743_002207"
     CDS             complement(5475..7967)
                     /locus_tag="RD743_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDU0564007.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7974..8441)
                     /locus_tag="RD743_002208"
     CDS             complement(7974..8441)
                     /locus_tag="RD743_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDU0564008.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2355 a         1814 c         1422 g         2983 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1081 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1141 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1201 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1261 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1321 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1381 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1441 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1501 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1561 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1621 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1681 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1741 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1801 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1861 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     1921 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     1981 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2041 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2101 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2161 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2221 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2281 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2341 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2401 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2461 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2521 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2581 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2641 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2701 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2761 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2821 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     2881 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     2941 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3001 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3061 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3121 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3181 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3241 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3301 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3361 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3421 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3481 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3541 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3601 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3661 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3721 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3781 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3841 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     3901 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     3961 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4021 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4081 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4141 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4201 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4261 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4321 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4381 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4441 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4501 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4561 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4621 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4681 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4741 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4801 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4861 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     4921 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     4981 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5041 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5101 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5161 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5221 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5281 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5341 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5401 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5461 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5521 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5581 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5641 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5701 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5761 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5821 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     5881 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     5941 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6001 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6061 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6121 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6181 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6241 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6301 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6361 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6421 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6481 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6541 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6601 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6661 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6721 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6781 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6841 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     6901 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     6961 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7021 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7081 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7141 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7201 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7261 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7321 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7381 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7441 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7501 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7561 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7621 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7681 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7741 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7801 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7861 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     7921 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     7981 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8041 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8101 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8161 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8221 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8281 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8341 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8401 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8461 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8521 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DAPCMT010000115         8572 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPCMT010000115 DAPCMT010000000
VERSION     DAPCMT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.117"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(50..133)
                     /locus_tag="RD743_002209"
     tRNA            complement(50..133)
                     /locus_tag="RD743_002209"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Leu,seq:cag)
     gene            661..1161
                     /locus_tag="RD743_002210"
     CDS             661..1161
                     /locus_tag="RD743_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0564009.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1351..1695)
                     /locus_tag="RD743_002211"
     CDS             complement(1351..1695)
                     /locus_tag="RD743_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDU0564010.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1849..2769)
                     /locus_tag="RD743_002212"
     CDS             complement(1849..2769)
                     /locus_tag="RD743_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDU0564011.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2956..3447
                     /locus_tag="RD743_002213"
     CDS             2956..3447
                     /locus_tag="RD743_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDU0564012.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3471..5846)
                     /locus_tag="RD743_002214"
     CDS             complement(3471..5846)
                     /locus_tag="RD743_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDU0564013.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6172..6798
                     /locus_tag="RD743_002215"
     CDS             6172..6798
                     /locus_tag="RD743_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDU0564014.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6821..7525
                     /locus_tag="RD743_002216"
     CDS             6821..7525
                     /locus_tag="RD743_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDU0564015.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7717..8550
                     /locus_tag="RD743_002217"
     CDS             7717..8550
                     /locus_tag="RD743_002217"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDU0564016.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2606 a         1641 c         1528 g         2797 t
ORIGIN      
        1 tctttctcat cgctccaggt gtaccttctc tttataaaaa aacaaattat gcggctaaga
       61 agactcgaac ttccacgagg tcaccctcac tagcgcctga agctagcgcg tctgccaatt
      121 ccgccatagc cgcatcacat cttttagttt aggattacat gtgttttttg tcaagaacat
      181 atttttgttc tttttcattt ctgtacgccc tcccttatgc catctacaca aataattcat
      241 ccgagcaact ccatcatcta ttgagggttg gtgaaaaaat ctgttttatc catatgatcg
      301 atttcaatgt taaacgaacg actctgggat tagtaactat tttatttttt tcttcatttc
      361 ttttttcctc tttttctcga ttcagctatc ttctttcatt tggatattga ttatttcttc
      421 tgttgattac tatccttctg gaattctaga cgctgaagta attcacgttt tctttcttct
      481 agagatcata agtaacgaat gaacgaactt caaataattt ttttattgta attcatattt
      541 gtattgttcg atgattctta atacgatatg acacaataaa taaaaatagg catggctatt
      601 tacaacaata gtgctatagt agaaaacaaa tgaataaggt tacttaaaga ggtgaatacg
      661 atgaaactga tcaaactatg ggaagcagat ttaaagaaag cttatcagct acaaactagc
      721 tttggaccaa atgagagtgg atttatcaac aatgcttatg gctatacatt tgctgaattt
      781 ttagattatg taaaaaaatg cgaaaaaacg tctttaggaa tcgatcttcc tgagagttac
      841 gttcccgcaa ccgtctttat tttagaaagt gatgaacatg agtatgtagg aatttttaat
      901 ttgcgccata aactgaacga aacattaaga aacggcgctg gccatattgg ttacggcatc
      961 tcccctaatt atcgtaaaaa aggatatgcc acacaaggat tagcattagt actacaagag
     1021 gcaaaaaaaa tcggcattga tgaggcactt ttgtctgtaa ataaaaaaaa tcttggcagt
     1081 cttaaagcac aacaaaaaaa tggggctgcg atttatcgtg aagatgagaa agaatactat
     1141 acgaaaatca aactgatgta aaaaagaagg attccaaaag gaaagggatt tgaatgtcct
     1201 ttatcaatag aacacattta tttaattaac ttataatata gcagacaaaa ttttataaac
     1261 aaagaaacct tttatctctt ttcttctaga aaggataaaa ggcttttttg tttttcctat
     1321 ttgcttctca tagctagtca gccaagattt ctatcgtatc cccaacgcaa tccgtgcata
     1381 acggctcatc ttatctgtcg tccatgctgg ataccagact agtttgactt ctgctttcgt
     1441 tacttctggc acttctttta atgcgtcatg gatagattct gttaaaatat ctgctaatgg
     1501 acagcccata gttgttagag tcatcttaat gactgtttct ccattttcag gattgaattc
     1561 tatatcataa atcaagccta aattgacgat gtcgattcct aattcaggat caatcaccat
     1621 ttccaaggca gcaagaatac gttctttgat tagttctatt tcttgtccag accgttcttg
     1681 gtttgcttcg ctcatcgtac gacactcctt aacacgattt ttatttatca tactcttttt
     1741 ttagctgctt gtaaatgaga atttagaagt aagattctcg tttaaattta taaaaatgtt
     1801 cttattagat gtaatagatt aaatttattc taaaataaac ctgattcctc attttttttg
     1861 tgaacgattt tttatttctg gcaatacgcc agttgttcga tacatttttt gttcgttttc
     1921 atttgcaaac cggccaaata ctgaatgagt tggtaaaata ttgataaatg catcgtcgtc
     1981 gatttcatga acgatctgat caagatcata catttcgtag cgggtgatga ccatcatgat
     2041 catgttccct tgtttttttg aatagccacc gacagacggc agtaatgtca ttccacgtac
     2101 catccgtttt gctagttctt ctgttacctc ttctggcttg acggtaacga tcattgctgt
     2161 tactttttga tgactcgtgt ggatggcatc aattacttga gtcattgcat aaatggaaat
     2221 aatcgtatac aaggcacttt cccagttaaa gaagaagcct gcgatgacta caatgatacc
     2281 atttagaatc agcatgtagt ttccgactgt ttttccagtt gtttgtgaaa gtacaagaga
     2341 gatgatatcc atcccaccag ttgtaaagcc catttttaaa gataatccta cgccaagacc
     2401 taataaaaca ccgcctacta gggcattcat caaaatatta tcagtgatct gtccgactgg
     2461 aagcagcatc gtcatcaccg atacacatac aacattcaca aaactaagga tcgttgctcg
     2521 ttttcctact ttgagaaacc ctaatacaaa cacagggata ttcaaaagaa aaataaatgt
     2581 tcctgtgtct atcttgatat gaaaccattc atataaaagc gaggaaatga tttgagcgat
     2641 cccattcatc cctgcagaaa agacattggc tggaatcaaa aaataattta aagcgaccgc
     2701 agcggtcaat ccggttatca aaataaccac tattttcttt aatctttcat ttttatgata
     2761 cgcagtcata attttactca caagcacctc tcccctactt tggtattcca aatgtaagaa
     2821 taacagttta tctattaaaa taaaatactt cgaactcaaa tgagaagaat tttagcaaac
     2881 aggtaagaat ctgccctaat cctatgataa gatagatgta tggtaaatat ttatatgtat
     2941 atcaggaggg ttcctatggc aaaagatgct agcttcgaca ttgtatcaga agtcaataca
     3001 gaagaagtaa aaaatgcgat tcagcaaaca acaaaagaat tgtccaatcg ctttgacttc
     3061 aaggattcta cagtagaaat caaattagag aacagcaatc tagttgtatt gagcgatgat
     3121 gatttcaaaa tcgaacagat caaagatgta ttatttggta agctgatcaa aagaaatgtg
     3181 ccaatcaaaa atatccattt ttccgaatca gaacacgctc taggtggcaa agctcgacaa
     3241 actgccgatt tggtcaacgg aatcgatcgt gaaaacgcga agaaaatcac tacagctatc
     3301 aaaaatgaaa aactgaaagt aaaagcacaa atccaagaag atcagattag agtgacaggt
     3361 aagaaccgtg atgatctaca agctgtgatt gctttattac gtaaacttga cttaccaatc
     3421 gagttgcaat tcactaacta tcgttaatca aaaaacgagg ctgacgtctg tcattcctcg
     3481 tttttttgat ttactgcttc ttttgataac caaaaacgct gtattccttc ttccaattcc
     3541 ccaacacttc gtacttctct tgctgtttgc caatgttgtg ggaataaacg tcgataagaa
     3601 aaactagaaa atcgttgatc tgccaataca acgattcctt gatccgccat gccacgaatc
     3661 actcgaccac cagcttgcaa gactttgttc attccaggaa gctgataagc gtaagcaaat
     3721 ccaaggtgtt ccttctcatc ataatatgat ttgatcagtt cctgctcatg attcatctgc
     3781 ggcaaaccta cacctacgat catactgccg atcaatcggt cccctctcaa atcgattcct
     3841 tctgaaaaaa tcccgccaag tacgcaaaat ccaatcaatg tttcagcggg atttgtttga
     3901 aattttgtta aaaatcgttc gcgttcttcc tcattcatgt ccgtttcttg gattagcaca
     3961 ttgctgtcgg gatattgctg tctgaatgct tccaccgcaa gatctaaata ttgataagaa
     4021 ggaaagaaaa cgaaataatt cccgaccttt ccatcagcaa aagcatgaat cgtctctact
     4081 aattttgcca gactgtgttc cctttttcga taagtcgtct ctaggtaatt tgcgatgaaa
     4141 acttgctggt ttccttctgg gaaaggactc ggcagtttgt aagccagact tttttgtccc
     4201 cccagtgttt cctgataata agagagtggt gaaaaactag cagaaaaaag aatactgctt
     4261 tttcctttat ccaacgactg ctcaagaaac aagcttggat caatacaaaa ctcttttact
     4321 atcagatcgc gatacgtttt ttcgactgtt gtttcgtagt gatcatcata aaattcactg
     4381 accttcaaaa aatgaagcaa atcaaaataa taaggaagca attgttcttg tgatgggtgc
     4441 tcaggaaatt ctgccagcca ttctttaatt ttttcgctta attgataacc tgcctttact
     4501 aaagattcag ccggtgcttt ttgatgatga taatcccaat gatcttcttt tgctatttct
     4561 cggatcgaat caaattcttt taatagtttg ttgaaacggc gatgaagttt tttgaattcc
     4621 tttggtatag cttccttact tcgtttcgtt ttttcataag atagttctgc cgaatacatc
     4681 tctcgggaac gattcaccaa attatgtgct tcgtcaatca aaaacagata gtcctcgttt
     4741 ttttcatctt caaaaaaacg tcttaaataa acagttggat caaataaata attgtaatcc
     4801 ccaatgatta cgtcacacca gatactgaca tctaatgata gttcgaaagg acaaagtgta
     4861 tgctttcttg cataagtttc gatgacttca cgagtgatct gattctcatg attcaaaaga
     4921 tcccataaac cttcattaat ccgattgtaa taaccattgg catatgggca ctgatctgga
     4981 ttacaggtcg tctcatctaa aaagcagatc ttgtctttcg ccgtcaaagt gacacttttt
     5041 acttcagctc ctgtatcttt taatgctgtc atagcatctt cagctacttg tctagttatt
     5101 gttttagccg ttagataaaa gatcttttct ccctcttctt cccctacagc tttcaaagcg
     5161 gggaataacg tagagattgt ttttcctgtt ccggtaggtg cttctacaaa caatttctgt
     5221 ttcgtacgga tcgttttata agcagctacc gctagttcac gctgaccttt cctatactca
     5281 tcatggggga atttcaaagc catcaaagag gcattgcgta cagtgcgcca gttttcttga
     5341 aaaacaagcc attctttata atcttcagtc agctttttaa aaaaagtttc taactgctct
     5401 cttgtctgat gttcaacctt tctcgtgatc acttcttctg ccgtctggaa gtaagtcaat
     5461 tgcagattta tttcttgtaa atcatgttct tgactataga tataggcata cacccttgct
     5521 tgatgaaaga aaagttccac ttgttctggt tctaaatctt caaatcgtgg ttcggaggtt
     5581 ttgatttcat ctatataata aataccgtct cttttgaaaa taccgtctgc acggccttca
     5641 acgatcaggt aatattcatc taattgaact gttgttttta aaaagacttc tttttcatag
     5701 ttttctcccg catctttttg gagcttgcgg tggattctcg ctccttctaa tgcagtatgg
     5761 ttactttttt tacgattgtc gatacttcca cgtcgcaaaa taaattctac taagtgccgc
     5821 acagatattt ttgactgttt gatcatttcc accacctctt tctctctaag attacctgaa
     5881 aaaacctagt ttagcaaaaa gagctctctt tccaaacata aaaaaacacc ctgctgatta
     5941 ggcagggata taggatatag aatttggatt tttggatgaa tctaatgtaa caaggacata
     6001 ttaaaatgct atgaaaactt actttccttc tctttagaat atccaaatac ttttcatata
     6061 gtctgataat tcagataagt aaagagaaat aagtacctgt ttttgtttcc tagcaatgaa
     6121 tgcgttatac taatggatga aagaagtttt ggctatcaag gaggaatgca catgcatatc
     6181 gaaagattaa aaacaggaat gattgaagaa aactgttatt tggtctacaa cgaagaagca
     6241 cttttgatca ttgaccctgg agaagatgcg gaacgaatca aacaagaaat caaaaaaaca
     6301 aaccaacagc cggtagcgat tttgcttacc catactcact acgatcatat cggtgcagtg
     6361 gaacctttac gcgcgtatta tcaaatccca gtttatgtca gcccactaga gcaaaaatgg
     6421 ctaggtgatc cgattttgaa tctttctgga ctcggtagac atgatgatat tgctgatgtt
     6481 attgtctcgc ctgcagaata tgaatttgaa atgaaaactt atactttagg aaacatgtct
     6541 tttcgcgtcg tccctacccc agggcattct ataggaagcg tcagctttat ttttgatgat
     6601 tttgtcgttt ctggcgacgc cctgtttaga ggaagtatcg gtagaaccga tctgtataca
     6661 gggaatttag aacaattact tcatagtatt cggacccagc tgtttgtatt acctgatgaa
     6721 tttgcagtct atccaggaca tggcgatgca acgacgattg aacaggaaaa aaggacaaat
     6781 ccttttttca atcgttaaaa cgacttagga ggagaataaa atgacagaaa ctacacttta
     6841 tatcgtacga cacggaaaaa cgatgttcaa tacaattgaa cgagtacaag gctggtgtga
     6901 tacacccctc acaaagcaag gacaagaagg cattcattat ttaggtcgcg ggttaaaaaa
     6961 tgtcgacttt tcatttgctt attctagtga cagtggtcgt gcaatcgaaa ctgcccgtat
     7021 tattctagga gaacattcta agggaaaaga aattccttat tttattgaaa aaaggattag
     7081 agaatggtgt ttcgggtctc ttgaaggtgg atatgacatg gaaatgtggg gtgtgatccc
     7141 tcgtgtattg aattttaaaa catatgatga aatgtttacc acagaagttt cttttgaaca
     7201 aattgctaat gctgtataca acttagatac cgctgggtgg gcggaaactt atgaaacatt
     7261 gcgagaccgt gtatggacag gatttgaaga tatcgcccat cactgcgaaa aaaatggtgg
     7321 tggaaatgtt ttagtcgtat ctcatggttt gacgatcgcc tttttgctta atctgatcga
     7381 tcctaaacag cctgttcgtg ctgggctttt aaatggcagc gtaacaaagg tcacttatgc
     7441 aaacggtcga ttttctatcc acggcatcaa tgatacgtct tatatcgata acgggaaaac
     7501 acatagtcgc gactcgtttc tataatgggg atattttaat ttttaccaga gaatcataaa
     7561 aggcaaagtc tgaacgaggg ctttgtcttc ttttttctct tcgtgtactt atctgcttta
     7621 tttctcaagt ttttcttaat attaaaaaac agattgggtt tctcaaaaaa taacggtata
     7681 ctataagatg tatcttaatt gggaaggaag gaatttatgt tttttgcaaa tattatcaaa
     7741 gcagtgattc tcggtattat tgaaggaata acggaatggc tgcccatcag tagtactggt
     7801 catttgatcc tagcagatga attcatcaaa ctggatgcca gttcacaatt tatgtcgatg
     7861 ttcaatgtcg ttatccaatt aggggcgatc ttggctgtcg tcgttcttta ttttcacaag
     7921 ttaaacccat tttctccaag taaatcaacg gtagaaaaaa aagatacgtg gacactttgg
     7981 tttaaagtga tcgttgcttg cataccagcc atcatcattg gtttgccttt ggatgattgg
     8041 ttagaagcaa atttccacaa gttcttacca gttgccattg ttttgatcgt ttacggtatt
     8101 gcctttatta ttgtcgaaaa acgcaacaaa aacaaaacac ctcgttggac ttctctgaat
     8161 gactttacgt accaagcagc tcttcttgtt gggttattcc aagtcttggc acttgttccc
     8221 ggaacatccc gttctggcgc aacgatccta ggatctatct tgattggtgc ttcgcgtttt
     8281 gtcgctacag agttttcatt tttcttaggg attcctgtca tgtttggcgc aagcggcttg
     8341 aagatcgtca aatatttagc tgaagggaat tcattcaaaa tggaagaaac tgttgttctt
     8401 cttgtaggaa cattcgtatc ttttgtggtt tccgtcttag ctatcaaatt cttactaagc
     8461 tatttgaaga aaaacgactt taccgctttt ggctggtatc gtattatcct tggtatcatt
     8521 ttgattggtt attggtttat tgcaatgtag aaaaaaccag aacacttctc tt
//
LOCUS       DAPCMT010000116         8494 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPCMT010000116 DAPCMT010000000
VERSION     DAPCMT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.118"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..173)
                     /locus_tag="RD743_002218"
     CDS             complement(<1..173)
                     /locus_tag="RD743_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0564017.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="RD743_002219"
     CDS             complement(593..1714)
                     /locus_tag="RD743_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDU0564018.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="RD743_002220"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="RD743_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDU0564019.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="RD743_002221"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="RD743_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDU0564020.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="RD743_002222"
     CDS             complement(4304..5215)
                     /locus_tag="RD743_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0564021.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="RD743_002223"
     CDS             complement(5232..6239)
                     /locus_tag="RD743_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDU0564022.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="RD743_002224"
     CDS             complement(6236..8365)
                     /locus_tag="RD743_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDU0564023.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="RD743_002225"
     CDS             complement(8370..>8494)
                     /locus_tag="RD743_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0564024.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAPCMT010000117         8350 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPCMT010000117 DAPCMT010000000
VERSION     DAPCMT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.119"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..450)
                     /locus_tag="RD743_002226"
     CDS             complement(<1..450)
                     /locus_tag="RD743_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDU0564025.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDL"
     gene            complement(580..1290)
                     /gene="budA"
                     /locus_tag="RD743_002227"
     CDS             complement(580..1290)
                     /gene="budA"
                     /locus_tag="RD743_002227"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDU0564026.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1303..2967)
                     /gene="alsS"
                     /locus_tag="RD743_002228"
     CDS             complement(1303..2967)
                     /gene="alsS"
                     /locus_tag="RD743_002228"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDU0564027.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3175..3837)
                     /locus_tag="RD743_002229"
     CDS             complement(3175..3837)
                     /locus_tag="RD743_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDU0564028.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3847..4662)
                     /locus_tag="RD743_002230"
     CDS             complement(3847..4662)
                     /locus_tag="RD743_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0564029.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4923..5366)
                     /locus_tag="RD743_002231"
     CDS             complement(4923..5366)
                     /locus_tag="RD743_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDU0564030.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5500..5838)
                     /locus_tag="RD743_002232"
                     /pseudo
     CDS             complement(5500..5838)
                     /locus_tag="RD743_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5826..6203)
                     /locus_tag="RD743_002233"
     CDS             complement(5826..6203)
                     /locus_tag="RD743_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDU0564031.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6427..7620
                     /locus_tag="RD743_002234"
     CDS             6427..7620
                     /locus_tag="RD743_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0564032.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7786..8208)
                     /locus_tag="RD743_002235"
     CDS             complement(7786..8208)
                     /locus_tag="RD743_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564033.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2449 a         1688 c         1413 g         2800 t
ORIGIN      
        1 aagatctaca tatttttcta gaaatgcttg atcgatttca tcataccctt tcgttactcc
       61 gcaatcgata tctaaaacac cgattaaatt accttctttt accattggca ctacgatttc
      121 tgattgtgcg gctgaatcgc aggaaatgta atttgcatgg gttttgacat catcaacgat
      181 caatgtttct tgtttttcag ctgattctcc acaaactcct tttcccatct tgatacgtgt
      241 gcaagaaact cgcccttgga acggtcctaa aatcaattca ttaccatcga ataaataata
      301 tcctgcaaag acagtctcag gcaaagtctc attcaacaat gcagatgaat ttgctaaatt
      361 tgctaaccag ttatcttctg cttctaataa tccttgttgc tgtaataata aaatttcgta
      421 tgcatctttt ttctgttttt ctgtccacat gataaaaacc gctccttttt gatctatgga
      481 gaagattata gctcagtttt tttgtaaacg ctaggtttcc tcctcaaaaa aacgagatac
      541 gtcaggttaa tcaccttgaa cgcatctcgc tgtctttttc tattctgctt gttggatttc
      601 tgctgctaaa tcagcataat caatcgtttc gtgaacaaaa gtcggatcat caactggaaa
      661 atgctgtaca agggacgaga tgcggctaat ctcaactttt ccttcagcca cttcaaaatc
      721 cattacatgt ccgccaaatg tatgtgtatc atcgataaaa tgcaggtgaa atcccgctgc
      781 agcaactcct tggaataatt caggcgtaaa gaacccgact aatgttcctt ggatattgct
      841 ttgttggaat tctggttgag actctgaaat ttttgctagt cttctgtaag gtttttcttg
      901 cttcggcatg atacggatgt gcatattttt gaataatcca gttatcttca ctgccaaaaa
      961 taggttttcc ccggcttctc ttactaatat gtcttttttc aattgttctc catctgcagt
     1021 ttttgagaca gaaaaactag tatctggcgt aaaatctgta attgctgcat agggggtaag
     1081 ttcacttcca tctaattggg caactgagcc atctgatctt ccttgataag ctacgccatc
     1141 taaaaagatg acttcaccat caagaccatg caatgttccc aatcccagag ttcctttttc
     1201 taatatatga gcaatcgttt ctgttccatc catcaagccg gccatcaaag ctcctaatgt
     1261 cccgtgctgg tataagattt tttcagtcat gtcgacactt ccttaataga attgatctgg
     1321 taagattgtt tctcccaatt tctcattatc acggtaatca atcggaatat caataattac
     1381 gggtccttct gtcgcaaatc cttcttctaa agctttttct aattcctcta cacttgtagc
     1441 tcggattcct ttagcgccaa aagcttctgc atatttcaca aaatcaactg gaccaaagtc
     1501 tacacctgac gcccgctgat atttcatttt ttcttggaac tctaccatat tgtagtgtcc
     1561 atcattccaa atcaaatgaa cgatattcaa ttttttacgt accgccgttt ctaaatcctg
     1621 ggcagagaat aaaaatcccc catcgccgga aactgatacg atttgtgtat ttggacgcac
     1681 caatgcagca gaaatcgccc atggcaatgc aacaccaagc gtttgcatac cattactgaa
     1741 taacaaatga cgaggttcat aacttctaaa gtggcgtgcc atccaaatat aatgactacc
     1801 tacatctacg gtaacagtca tatcatctgt tacttttgac tgaagcgtgt taataacttc
     1861 taaaggatgc aagatacctg cttctccttt tgattgaaca atgtcccgct ccgttaattt
     1921 tgcttgaaga gatgctaaat attcttttgc atcttctgac acttgttgag gctctaatga
     1981 acctgtcaat aaatcaagag tggctgagat atcaccgatc aattctcttt caggctgcat
     2041 aaaaggatcg atttcagctg gcgcttcatc gatcacaatg atccgcgcat ctttttccgc
     2101 attccagttt cttgcttcat attcgattgg atcatagcca attgcaataa ctagatcact
     2161 tcgtttcaaa agcatatctc ctggttggtt tctaaataaa cccacacgac caaagaaatg
     2221 cgcttctaat tcacgtgaga tcactccagc tgcttggaat gtctctacaa caggcaatgc
     2281 tgtcttttca actaattgcc tgatggctaa cgtttctttt tcacttgatc ctcgcatacc
     2341 tactaatagt accggtagtt ttgcttctct aatgcgttca gctaggtaac ggatatcatc
     2401 tgcagaagca gaacctaatt taggatcgct caatggcttg atgacattac cttggacagg
     2461 tgcatccact acatcttgcg ggatactgat aaagctcgcg cctttttttg aactcttagc
     2521 catacgataa gcattggcaa tcacttctga aattgtttct ggatcttgga tttctgcact
     2581 gtattttgtg attggctgga acagtgctgc attatccatg ctttgatggg tcaacttcaa
     2641 tagatcgctt cgttttactt gtccggcaat tgctaaaact gggtcacctt ctgctgtcgc
     2701 tgttaccaaa cctgtagcta agttactagc accaggacca cttgttgcga taactactcc
     2761 tggctctcct gtaatacgtc cgattgcttg tgccataaat gcagcatttt gttcgtgacg
     2821 agtcacgatt aattcaggac cttgatcctc taattcatta aatacaccat cgatttttgc
     2881 acctggtata ccgaatacat aatcaacatg atgattaatc aaactttcta ctacagattt
     2941 cgaaccttgt ttcatttttt cagccattga acatcgtcct ttttatgctt tatttttaat
     3001 tattagttag aaataatcac aaataagtga tacagtttct aaacctgttt catacaaaag
     3061 tatagcacgt tttatttcat ttgtttagcc atatgtttca aaaaatgaaa aagaatgaca
     3121 gtttttacat aaaaaaaact gtcattctta attgaaagtt tcaatttata aagtctacag
     3181 atccatagtt ctcatcaatt tacgattgtt gaagtaaatc aaaccaaata cgatagaagg
     3241 acctagtaga atacaccatt ggggctttac gaaaaagaag agaaacaaac ttgtcaatag
     3301 taaaaaatct agtaaactca cataccattt tttcaacgta tagtagaacg ctatccgtag
     3361 aacttgattt ggtttcgacg ctggatttct cacctgaaaa taccagcagt tggtgctaaa
     3421 ggaaactgat aaacaagcaa gtaaaacaaa caacggaatc aaccattttc cataagaaaa
     3481 acgtataaaa aagaaacaat caaaaatcat tatcacacta actatccaag caaatagcca
     3541 ataaaaaaat ccctttagcc aaaattggcg aaacatcttt atgaacaaac gtactggtga
     3601 aaaactattt tcttttgcta attcttctgt catacctatc atcacgatgc aacctggacc
     3661 aataaaaagt aaagaaaaaa taaaggaaaa gacattcctt ccatctatcg acaaaaggaa
     3721 cgcagctgct atatacaaga tattgacaca ccaaaaacaa aggctacttg ccaatgattg
     3781 atacaaccaa taaaagaatt ccatataaac atttttgtca aaacgctgct ctttcataat
     3841 cacctcctat tccttcattc ctgatgaagc gatcccttct acaaaatatt tttgcatcgc
     3901 taaaaatata aaagcaacag gaagaacaga aacaactgag gcagccatga ttaatgcgta
     3961 ctctgcatca aatagcccaa taaacatttt caaccctaac tggattgttt tattttccgt
     4021 agaagacaaa taaatgaatg gccccatata atcgttccat gtattaacga atgtgatgat
     4081 cgtcaaactg gcaatcgccg gttttgtaag aggtaaaatg atcttgcgat agattcccca
     4141 ctctgataag ccatcgatcc gcgcactctc acataaagaa tctgggatac tactgtaata
     4201 ttgcttcaac aagaacacac caaaagcatt gaacgcttgt aatagcacca acgaccacaa
     4261 cgtatctgtt aaacccactt ttctcatcat aatgaactgc gggatcatat aagactgcca
     4321 agggacagca atcgtgccga tatatgctaa aaataaaata tcgcgccctt taaaatttag
     4381 tttagaaaac ccgtaagcag caaaactaga agtaaacaac tgaatcaacg tgatcacaac
     4441 gcttaaaaaa gctgtattct taaaaaatgt cactaaagga atacgagacc aaataaccag
     4501 ataattctct aaatgccaag ttttagggat ccattgaatc ggaatcgtga acacttcatt
     4561 atttgctttg aatgaagcag aaaccatcca taaaaaagga atgatcgtca ataaagctaa
     4621 tacaatcagc agaatcatac ccaaccattg aataaagggc atgttttttg aacgaatttt
     4681 tgcttttttt ctttcatttc gaacacctcc aaagttataa tcttcatttc tttgtaatga
     4741 ttacaatcca gaacaacggt tattatagct gattcatttc cgttcttctt atttttgtaa
     4801 gcgcttattt aacaagttat gtaaatctct ttattttttt gatttaaaaa tgaaaaaata
     4861 caaaagcaag actgtcatac gtctcgcttt tgtattgaaa tttcttcgat tagagatact
     4921 tattactgtt caaaagcttc tttgaaacgg tgaatcaatt cattgactac aggtgataaa
     4981 atccgattct ttttccagac tagcatgcag ttcgtttcaa tcattgggtc aagcggaacg
     5041 aatgtcactc gctttgaatg atgacttaca gcaccttcta tgactaaagc agcacctacc
     5101 tgattctcaa ttagcgcaaa tacattgaaa atcaaattgt acgttcctgt gatcagcaac
     5161 tgctcctttt ttatgcctaa ccagtcagca aacattcgct gtatttctgg acgctttgag
     5221 atcaaaagag gtacattcgc aaagtcttct tttgttatca cgtctttttt tgctaaaaca
     5281 gaattttcta aaatgagcaa tccccatttt tcctttcttg gtaacaaaat cgtttcgtat
     5341 ttctctgtat ctactggatc tagcaaaatc gctaaatcta acaggctttt ttctagtcac
     5401 tccgtgatat catcacttgt tccgctgtgg atatgaaatt gaacgctggg ataatcacgg
     5461 atcaactttt ctagtaatag tgccaatgta ttcgaactat cagcttccac acagccgatc
     5521 gtaaatcggc cgctgaataa ttgttttttt ctgttctcga attcttgagt ggtttgattc
     5581 gttaaatcta aaatttcttc tgctcggctt ttttagaaaa taacctgctt ctgtcaagat
     5641 cattttgcgg ttttcgcgta caaacagttc tgttcctagt tactcttcta actccttcag
     5701 ttgtcggctt aaggttggtt gtgtaagatg tagaacttct gcggcttgag agattgttcc
     5761 ttcttctgca attgtccaaa aatattttag taagcgtaac tccatagcga tgctccttga
     5821 tttttttact tgatacattc cattttattt ctagataaca cacgatcata tttcttgatt
     5881 tttccttgta aacgttccaa acattcttgc atttcagcaa tttttttcgt caaaagttca
     5941 ctttcttcaa tcaaaagctt acgccgctca aatacagttg cttctccttc gctagatagg
     6001 cgaatatatt caatcaaaga ctcgatcgat aatccggcat ttcgcataca gatcgtaaag
     6061 tcgacccatt ttaggtcttc ttctgtataa tcacggattc cgccttttgt ccgattaact
     6121 ggtggaatca acccgattcg ttcataatag cgcaatgtat cggccgaaat gcccttttct
     6181 tcactgactt gtttgatatt catctgatca ctctttctct taatttcttg ccactttact
     6241 tatgtatttt atttattcta caactttaag tgtactccaa gtcaatactc atttctatta
     6301 aaactaagct ggattatcta tatcggttgt ttgtgtatta tttcaaataa aaaagtcttg
     6361 tttttttatt cttttttgga tatactttta acaatttagg gaaattttag gaaaaggggt
     6421 ataaaaatga caacgtatca gcgaaaggta ttagtatcga catcggcggg aattgcttta
     6481 gagaatatgg atattatgtt tttagctttt tcattatctt caatgattac ttcttttcat
     6541 attagtggta cgcaagcggg tttcatttca acgatcacta atttggggat gctcattgga
     6601 ggtatttttt ttggcatcat agctgataaa tatggacgtg tgaaagtatt ttctcaaacg
     6661 gtccttctgt tttcgattgc ttctttattg atgtattttg cttcaaacat ctacttggtt
     6721 tatctctttc gctttatcgc aggtgtagga gcgggaggag aatatggggc gtgtatgtcc
     6781 ttgatttccg aaagtttttc aaaaaaacaa attggacgag cttcttcggt tgctggtatt
     6841 ggtgcgcagg tcggtgcggc tttggctgcg attttagctg ctgtgatcat tccaacgttt
     6901 ggttggaaga tgttgtatgt cgtaggtgta ctgccggtgt tgatggtctt gttcattcgt
     6961 cgcggcttga ctgaaccaaa ggaattcaaa tctgtacaat ctgccgaaaa gaaaaccaaa
     7021 cttagcagac tgtttgaatc aaaacatctt gcttggcaaa catgtggcct tagtatcatg
     7081 gtaactgtcc aaattgccgg ctattttggg ttaatgaatt ggctaccttc aattatgcaa
     7141 aatcgattag ggctttcggt ttctgggtct tcattgtgga tggtcagtac tattttaggc
     7201 atgtctttgg ggatgatgac ttttggcata atcatggata aattggggcc taaactagcc
     7261 ttttctttgt ttttaatttg ttcatcctta tctgtctatc tacttgtgtt agcccatagc
     7321 cagtggagtt tggtagcagc cgctgtgata gtcggctact ttatcaatgg catgtatggt
     7381 ggatatggcg cagtgattag tagtttgtat ccaacagaaa tccgcgcgac ggcgaataat
     7441 ttcgttatga accttggacg agctgttggt ggtttttctt ctatcgtcat cggtttcttg
     7501 atggatcatt atagtttaac tgccgtaatc ttgtttttga gcggtattta cctagtcagt
     7561 ctgctggtct tgacaactct tactggtgtt acagcattaa gggaaagctt gcagcattga
     7621 tagttgagtc tgtaaatgac aagaaagagg tgtggaaaaa gcttgcatct gcggtaataa
     7681 gaccagccaa atcaaaaaca tgagacactg tttccgtttg gctggtcgta ttacttgatg
     7741 cagggcagct ttttcaactg ctaatttcct tctatctggc ggcgactatt ctttgacgtc
     7801 cgtcatttca aagtacttta ctaataactt agcataacga aaaactgtgg atacactcgt
     7861 atattgagcg tctgcataat cttccaaatt tttctcagga tgttgataca tgaattcaat
     7921 caaatgataa ttatatgatt ttctgtagaa atgaagataa ataatgtaga ttgggaagtt
     7981 ctcctgcttt tccatagaaa agcgccgatt attctcattc ttcaaaatga ccttatcagg
     8041 atattgttgg aacaattctt ctaaatctgt tagataattc aagatagact tacttgatac
     8101 cttcaattcg ttttgtagtt cttctattga acaccaacgt tctgtttcaa gcagtaaatg
     8161 aatcatttta attttctttt gagaaacttt tgttaaaaaa gtctgcatat tttctcctcc
     8221 tgtatctact ttactatact acatacaaga aaaaaacgga tacaaatatc atattgtcac
     8281 accgtcaata gtgcataaac ctgttattat ttctcttttt ttcctaagta ttctattgat
     8341 atgaatcatt
//
LOCUS       DAPCMT010000118         8340 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPCMT010000118 DAPCMT010000000
VERSION     DAPCMT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.120"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..446
                     /locus_tag="RD743_002236"
     CDS             <1..446
                     /locus_tag="RD743_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDU0564034.1"
                     /translation="RYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPI
                     FDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKK
                     GITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            461..1213
                     /locus_tag="RD743_002237"
     CDS             461..1213
                     /locus_tag="RD743_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0564035.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1916..2131)
                     /locus_tag="RD743_002238"
     CDS             complement(1916..2131)
                     /locus_tag="RD743_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HDU0564036.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2150..2386)
                     /locus_tag="RD743_002239"
     CDS             complement(2150..2386)
                     /locus_tag="RD743_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HDU0564037.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2644..4797
                     /locus_tag="RD743_002240"
     CDS             2644..4797
                     /locus_tag="RD743_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HDU0564038.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGE
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4766..6181
                     /locus_tag="RD743_002241"
     CDS             4766..6181
                     /locus_tag="RD743_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDU0564039.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6214..7248
                     /gene="sppA"
                     /locus_tag="RD743_002242"
     CDS             6214..7248
                     /gene="sppA"
                     /locus_tag="RD743_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDU0564040.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7250..7771
                     /locus_tag="RD743_002243"
     CDS             7250..7771
                     /locus_tag="RD743_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDU0564041.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7768..8154
                     /locus_tag="RD743_002244"
     CDS             7768..8154
                     /locus_tag="RD743_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDU0564042.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2977 a         1198 c         1499 g         2666 t
ORIGIN      
        1 atcgttattt aaaaaatata aaaaatgaat atttgaaaag tttgttgata gcgaagctat
       61 accaagcaaa taacaataag atttgttgta aattcgaatg tccaagtact attattgaag
      121 ttcctgttcc aatatttgac tgtattaggg tgcttggaat tattctcgat aatgcaattg
      181 aagcatctat agagagtcaa gatagagaac tgtctctagc tatttaccga gatgataaac
      241 agatagaaat ttcgatatcg aatagttgta gagagatact actctctata gatactctaa
      301 tgaaaaaagg aataacaaca aaaaagggac atcaagggtt aggtttatct agtatagagg
      361 aaatcaataa aaagaatccc aatatgtttg tgaactatca aaacgatccg tatcaattta
      421 ctactcaagt tattttgttg ttttgaggga aggaggattt atgagttatc caataatatt
      481 atgtgaagat caaattatac aattaaatca gttagaacga attattgata atttcattct
      541 ttttcacgat aaagtattta agatagtatt gaaaacacaa agtccgttag aagttaaaaa
      601 atacctcaaa caattccgac caaagcaagg aatatatttc ttagatattg atttaaatca
      661 tgaagttaac ggtatagaat tagcagaagt aatcagaaaa tatgatgttc aagcaaaaat
      721 catttttaca actactcatg atgagatgtt acccgtaaca ataaaaagaa gagttgaaac
      781 gttaggattt gtaacaaaag atcaaacact agatgagtat cgaaacgaga ttgttgagtt
      841 attgttatta gcgcaagaaa ggatagatgc aacaaaacag gttacgaatc aagcatttgt
      901 tttttccata ggttctcaaa catttacttt tgatataaat gacatttatt ttgtagaagc
      961 ttcaaaatta ccacatagat tatcattgtg tactaaagat gggcagtacg aattttatgg
     1021 aagaattagt gagcttgaaa agaaatatcc tatgttaaca agaattagtc gagcgtgttt
     1081 agtaaatatc tttaacgtta aagaaataga ttttaaaaaa cgttcactat attttgattc
     1141 agagttggcc agaaacttta ctttagggaa agcacaaaaa attaaggaga agttgaaaga
     1201 aagcactgtt tagtggtttt ttctttaggg agattgcatt tttctaataa aatagattcc
     1261 ttcactagtg caaatttttc tgaatcctat taaagttgta ccaattttta tagctgttat
     1321 tagttacgtg cataacagca atattagtta ctggaggaat atttctatgg tctggaacca
     1381 aaaaagggcc aaatgaatag ttcttttctt gtttttatta agtaccataa aggggaatta
     1441 aaattaaatg tatggtggaa gatgcgaaag atttggcaaa ttctactaat ttttagaaag
     1501 tgtttttcat tttttagtta aaaaatttgt cctgtcaagt gcaatcctat gattgtactt
     1561 gataggattt gagataatta ctttcctgtt tttataaatt taacttgcag gaacaggctt
     1621 taatgcacat ccgacaagta cattaccaat acctcctgta acggccgaaa taggtcctcc
     1681 tgcagcacca actacagcgc tgcaagtcct tttgtaaaaa tatctctacc attacatttc
     1741 caaagaattt ttatctgact ttaataaaag ataaaaattt aatataatta taaaaactag
     1801 cacaagaagc tttagtaaaa agttctgtcc attccaaact tttgaaaaga taaagaacat
     1861 acctatgttc gtaatcgaca caaaccaatc ttgaggtttg atatttttta attgtttacg
     1921 caccggtcca catgctgaat ccagcaccta ataaagctcc acctacacca caagcagcta
     1981 ttgcataagg tcctccggct gaacaaagtt ttactcctgc tgttgcccca gaaaaagtag
     2041 tgcctaaaac tcccacggca ctcccacctt gaatattttt caactcttct gaagataata
     2101 taaatgttgt ttttgtgttt ttaatatgca tactacttat tctccttttt tatttttttc
     2161 gagcatccca gccagctgca gcacctccag cgacagctcc atataaccct ccaacaacac
     2221 cgcaagctgg tccgcaaatt gctgttccta aacctgcacc acctactgcc cctgtggtaa
     2281 gtccacctac aacttgcccc aattttcccc aagcattatt gcctccacca acagtttgtg
     2341 catttccaca atacttagtt cctttacatt ttgcatgttt aaacacgctt ctttaaataa
     2401 tattttttac ggtgctactg taacatacgt atttgattaa cagtaaaaat cttcctgaaa
     2461 cttatcaagt ttattcttga atagtaaaat ttccaaaata aatttgattc aggaatgaaa
     2521 aagttagtgt tcaagaactg ttttcaaagt ttttgtggaa ttatgagaca atattttcac
     2581 tgtattaaag ctatattgag aatttttcta taaaaatttt ttagaaggag ttgtgttaga
     2641 taagtgataa aatcatttcc ttttgtgcag caacaagatg aaaaagattg tggtgttgct
     2701 tgcctttcga tgattttaaa atattttaaa actgatatcc cgattcataa attacgggac
     2761 ctatctggga ctgatttaga gggtacttca gcatttggat tgaaaaagac atttgaaaaa
     2821 ttgtcttttg attgtatggc tattcaagca gatagtgaga tctggaaaga aagagatctt
     2881 atcttaccat taattgcaca tatattggtt gataacagct atatgcacta cgttgtaatt
     2941 tataaaacga agggaaacgt actatggatt gcagatcccg ctaatggaaa aaatagaaaa
     3001 acgattgaag cgtttcaaaa agaatggaca ggtattcttt tattacctat gcctagagaa
     3061 acttatattc ctattaaaga aaaagtagct ggattaagtt cttttttgcc tataatttgg
     3121 aaacaaaaag gattagtatt ccacatcgtt ttagcatcga ttttcattac gttttttggc
     3181 ataggaagct catattattt tcaaggtatt cttgattttt tcatccctaa tcaagcacgt
     3241 tcaactttaa atataatttc cattggcttg atcgctgtct atttatttcg ggtattgttt
     3301 gaatatagtc gtagttatct tcttattatt ttaggacagc gaatgagtat ggcaattatg
     3361 cttcagtatt tcaaacatgt gttaacttta ccaatgaatt tttttgctac gagaaaatca
     3421 ggggaaatta tttctcgttt tcttgatgcc agtaagatta ttgatgcttt agcaagtgct
     3481 acgttatcaa tatttttaga tattggtatg gtgttgcttg ttggggttac gttagctatt
     3541 cagaatagta cgttgttttt aatcactcta tgctctttgc ctttttatgt agttgcaata
     3601 ttcgctttcg tgaaaagcta tgaaaaatcg aacagggaag aaatggcagc cggtgcagct
     3661 gtaaattcta gtatcataga aagtcttaaa ggaatagaaa caattaaagc atatagcggt
     3721 gaagagaaag tatataaccg tatagaccat gaatttgtta aattaatgaa aaaatcattt
     3781 cgtacggtta cgcttgataa tattcaacaa ggaattaaac atttgattca attaattagc
     3841 agtgcattaa ttttatggct aggttcttat tttgttatgg ataatagaat tagtctaggt
     3901 caacttatca catacaatgc tttactagta tttttgacag atcctttaca aaacattatt
     3961 aatttacaag tgaaaatgca aacggctcaa gttgcaaata atcgtttaaa tgaaattttc
     4021 gcgattgatt cagaaaaatc gaaccagtct tttgaatata tgctttctga acaattattt
     4081 caacaagata tcactgtaaa aaacgtttca ttctcgtata gcatgaaggc cccaacgttg
     4141 aacgaaattt catgtacaat tccttcgcac agtaaaacgg ctatagttgg agttagtggt
     4201 tctggaaaat caactcttgc aaagttattt gttcgttttt atgaagcatc agaaggatcg
     4261 atccattatg gaaacattaa tcacttagac attccttttc agatattacg cgatcgagtg
     4321 acatatttgc ctcaagagtc atttttcttt agtggaacaa tcattgagaa tcttatattc
     4381 ggtttagata gcccaccaag tttcgaacaa ataatagaag tttgtgagat agtccaacta
     4441 aatgaattta ttaatcaaca gccattacgt tacgagacat tgttggaaga aggtggaaat
     4501 aatctttcag gagagcaacg acaacgatta gccttagcac gggctttatt aaaaaatccg
     4561 gacatattaa ttttagatga agcaactagt ggattagata ccttgttaga acatgcaatt
     4621 atgaattatc tcctcaattt aaaagaaaaa acgattattt ttattgcgca tcatttacca
     4681 atagcaaaag catgtgatca aattttagtt ctccatgaag ggcatttggt agaaaaagga
     4741 acgcacgatg aacttcgttt tgccgatggt atctatcaaa aattatggga aatctaggag
     4801 agggaaacaa tttatgcaga ataataattg gttcgaacat tctagtatct atactaaaca
     4861 acatcatagc ttctaccgtt gggtgttata ccctgtaatt ctttttttat gtattattgg
     4921 attgttttta acctttgcta aaaaagaggt tgttattcgg acaacggctc aattgaaccc
     4981 cgaaaaaata gagaagttac aagtgccatt agaagctaaa ataatggaaa acttcctaaa
     5041 agaaaatcaa tttgttcata aggacgatgt gttggttaga ttggattgtt ctttaataga
     5101 gaatgaaaag gctcagatag aacaagaaaa ccaacgtatt actcaacaaa taaagatggc
     5161 tcagctattt attgaaagta taagtaaagg aaaaaatttg ttttcaacgg atgacagttt
     5221 tggctacagt aatcaattaa agagcatgtt gtcagaaaaa gaatcactcc gctacgcttt
     5281 gaagcaaagt gaattaaatg atcaaaagca attagaagta tacgaaaaga caaaaagaca
     5341 actagaaaaa caaattgaga gttcagatag taaattacaa gaatggcaac aagtacaggt
     5401 agcttggagt aataatcaat cattaaaaga tttttcaaaa gaaatgatgg caaactatga
     5461 gaattggcaa gaacaactaa ataatgtttc tgatgatcaa aaaaatcaag tgaaactgac
     5521 aatttcagca agcataaatg aacaaattga gcaactaaaa aaagaagtag aacagtatca
     5581 gtcagaaaaa gctaaattag ttaaaccaac tacttctgag aatgacagaa ttagtcaaac
     5641 ggaaaaagga aagcaagagc tagaacaagc aataacaact gtaaaacaaa cagttacaga
     5701 gctaaatgaa aaacaagaga agaaccagtc agttatcaaa agtttggacg atcaactttc
     5761 aaaaggaata ctaaaagctc ctgtatctgg aacggttcat ttaaatgaag aaacaaaggg
     5821 acaagcagaa ctagccaaag gaacagtgct tgcagaaatt tatccgatcc acgaaaaatc
     5881 aaaaatgaaa tttacagcac ttttacctgc gaatgaaagt acctatatta aagaagggat
     5941 gaaggttcat tttaaattgg atcaaaaagg tggggctccg attacgatcg acggaacttt
     6001 agatgaaatt tcagaaaatt ctactgccac tgagaaaggt gttttttatg cagtgaaagg
     6061 aacgttaaaa acaactcaat cgtttccgtt tcgttatgga ttaactggag agttgtcttt
     6121 aattgtagga gaaaaaacat attggcaaca aattgtcgat gtgtttctaa ataaaaaata
     6181 attattttta ctaagaatat aggaggagtt tatatgaata aaagaagatg gattgctgtg
     6241 ggagtagcgt gcggattatt gttactatcc attatcgtcg cgctaattcc aggaaaagat
     6301 aaagaggaag catcaaatac aaccctaaca ggaataaata agatttttta cggatctaac
     6361 gaaatcacag aagaaacgtt agaagaaggc gcatcaaata aaaagattgt caaactatcc
     6421 gtcaatggag ttattgcaga tacaggtgaa agcaatcttt ttagtaggga acaatataac
     6481 catcagaatt ttttaacaca gctaaagaaa attcaagaag acaaagcggt taagggagtg
     6541 ttattagaag taaattcacc tgggggagga atatatgaaa gtgctgagat tgctaaagaa
     6601 atggcaaaaa ttaaaaagct agatattcct atctatactg catttaaaaa tacagcggca
     6661 agtggagggt actacatttc tgctggttca gataaaatat ttgctacgga agaaacaaca
     6721 actggatcaa ttggtgtcat tatttctggt ttaaattatt ctggcttatt agaaaaactt
     6781 ggcgtaactg atgcgacgta taaaagtggt gcattaaaag atatgatgtc acctcaacac
     6841 aaaccttcag aggaagagaa taaagttata caggaatttg tgatgagtgc ttatgatcgt
     6901 tttgtcaatg ttgtagcaaa aggaagaaat atggatacta atgcagtgaa agaacttgcg
     6961 gatggaagaa tttatgatgg taaccaagca gtagaaaatg ggttggtgga tcaaattggt
     7021 tactctgaag atgcattaga ttccctaaaa aaagagaaga aattaactga tgcgacggtt
     7081 atagaatata aaaatgacac aactggcttt gcttcttctt ggttaggtaa taagattgct
     7141 gaatggcaag ggctaaaggc aactacatca gatcgtgtaa tttcagtttt tgaaaaactt
     7201 ggattttctg atactccaaa accaatgtat tactatggag ggctataaaa tgaataagaa
     7261 gagtggcatt gaaactttgc ctaattatgt atttgcggga ttttggttac gattttttgc
     7321 ttttcttatt gatttaattt gtattagcat catcacccat gctactatag gtctctttta
     7381 caaaatcatg ggactggaat ctgacaaatc cattattact ttttatggta tagcgtcatt
     7441 agctatttat ttaacctatt ttgtactttt gactaagctg aattcaggac aaacaattgg
     7501 gaaaatgatt tttgggataa gagtagtgag ctttgaggaa aaagagctaa gttggcaaac
     7561 tatctttatt cgtgagtgta tatgtaggtt tatattaaaa aattttgtat ttcaaattgg
     7621 gtatttggtg gctgctttca cgcccaagaa acaacatatt ggggattatt tttctaacac
     7681 gtcagtggtt gttattcgtt cattagaagc tattaactat ggtcaaaaaa tttctaattc
     7741 taaaacactt aatctggaga gaattaaatg aataagagaa aaacagagtt tattttgatt
     7801 ttaattagtg gaataacagg gattttatgt agtcttttag cattgatggg tggtatgttt
     7861 ctatcttcta ttgattcaac tactttaaat actacagaac taaccagtaa ggatatagat
     7921 atgttagtca atctaagcgg gaatgttgtt ttatttggag gaattaccct agttttttcg
     7981 ataatgattt tcataatagg ttttttagta agaaaaaatc aaaaaacagt ggtctatggt
     8041 attagtttac tcattttatc tattattcca ttttttcttt taacattatt gtgggtaatt
     8101 cctggagttt taggtattat tgcgggattg atgcttctaa taagaaaatc ttaattaggc
     8161 aatgtgtaga caacgtgaaa cgattaatca attaaaagct aaatacgttt tgagaaatga
     8221 taacttgtag tcgatggaag acatgtagat aaaaaaacta cgtgtctttt atttattcta
     8281 attttttcga aaaaaagaca ctctctgatt gttgtattaa tggaaaaggg gaaagcagca
//
LOCUS       DAPCMT010000119         8318 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPCMT010000119 DAPCMT010000000
VERSION     DAPCMT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.121"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            125..676
                     /gene="lepB"
                     /locus_tag="RD743_002245"
     CDS             125..676
                     /gene="lepB"
                     /locus_tag="RD743_002245"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDU0564043.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(759..1259)
                     /locus_tag="RD743_002246"
     CDS             complement(759..1259)
                     /locus_tag="RD743_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDU0564044.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(1538..1936)
                     /locus_tag="RD743_002247"
     CDS             complement(1538..1936)
                     /locus_tag="RD743_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0564045.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2196..3056
                     /gene="ylqF"
                     /locus_tag="RD743_002248"
     CDS             2196..3056
                     /gene="ylqF"
                     /locus_tag="RD743_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDU0564046.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            3049..3816
                     /locus_tag="RD743_002249"
     CDS             3049..3816
                     /locus_tag="RD743_002249"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDU0564047.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            3872..4729
                     /gene="dprA"
                     /locus_tag="RD743_002250"
     CDS             3872..4729
                     /gene="dprA"
                     /locus_tag="RD743_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDU0564048.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            4851..6929
                     /gene="topA"
                     /locus_tag="RD743_002251"
     CDS             4851..6929
                     /gene="topA"
                     /locus_tag="RD743_002251"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDU0564049.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            6898..8274
                     /gene="trmFO"
                     /locus_tag="RD743_002252"
     CDS             6898..8274
                     /gene="trmFO"
                     /locus_tag="RD743_002252"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDU0564050.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2902 a         1455 c         1742 g         2219 t
ORIGIN      
        1 aaagtttttt gtccaatagc ctaattgaaa gaattatttt tatttcagct atctagtcgc
       61 atttttttga tatttttctt ataataaaga aagaatgaaa aaaagaggga tggattgata
      121 gagattgaca aaaaaacaac gctatattaa tcgcttctgg cttgtattca aatacttact
      181 tgtttctgta ttccttgcgt tcatgttgcg gggattttta ttcatcccgg taccagtgga
      241 aggcaattca atggaaaacg tgttgaagca aggtgatatg gttgtgatgg aaaaattttc
      301 ggagatcaga cgatttgata ttgttgtttt tcaattagca gatggcacga tttatatcaa
      361 acgagtaatc ggtcttccag gagaaaatgt cagctaccaa aatgatcaat tgaagatcaa
      421 tgggaaagta gttaaggaac cttatctaac taaaaacctg aaatcagatc atgcaaatgc
      481 atcctatacg acggacttta cactacaaga gctaacggga caatccaagc ttccggaaga
      541 taattatttt gtacttggag ataatcgaag agtttcaaaa gatagtcgtt cgtttggcac
      601 gatcaataaa actgatattc taggtaaagc aagatttgtc tattatccac ttgatgaaat
      661 aaaatggatc cagtgagaaa caataaagcg tgcagtctgt tgagactgca cgctttattg
      721 tttaatccaa aatcagttta ctaccttagt acgtaaaatc aaacagctcg tttaaacact
      781 tttctagcct ctaaagtttt gataaatgaa taaacaacca ttatttgaat agggatttgg
      841 atcatattcc tgatgaatct acctagaaaa attactttga aatctgcacc aaacaaaaca
      901 gacatcagcc aagaattgat aaaaaggctg actgggatca taatcaataa ttgggcagcg
      961 atgacggaag gcaggtttat ctttttatga tataaaaata agccattaat aaatccacca
     1021 aggaaagccg tgataagaaa agcagcttga aacgttcctg aaccaaatat cgtgaaattt
     1081 aaagtgttcg acaacatttc aagcaaacca gcccagatcg gtccatatag ataacccata
     1141 agcgccatac ttaaaaatcc gaatgtgatt tgaaaataag caggaccaat cgctatcttg
     1201 tataagatga catcaagggc taataaaaga gcagaggtcg acaacaatct tgcattcatt
     1261 tcgggcaatc taaaaacgtc ttttgcaatc aaagtgttca aaatattctc tcctttgtca
     1321 gttaataata aaaggatacc ataaaatcac caatgtgtca tgacacattg gtgatttaca
     1381 tacactggca tctaacaatt aaaaaaaggg tgagaagact gcttttataa actgtaaaaa
     1441 agagattgta cgaataaaaa aaatagaggc ggagaatact tatcgtatca tccgcctttg
     1501 tttttttgtt ataaaaaacg tcagattagt tatctgctta catcttcatc catacgggtt
     1561 ctaatgtatt tccgtctaga tcttggactt ctaatgaaaa catttcttct tcaggcattc
     1621 ccatatctac ttcgtggaaa gtaccaccat tttcttttgc aatttgagca aattttttta
     1681 cttcatccgc gttttcaaca ctaaacgaaa tcaaagcacc acttgttttt ttattatcag
     1741 ctatttcttt atcttttata aattttttat aaaaatcatg agagagaagc attatccaaa
     1801 aagtgtcatc ccataccatt gaaccagctt cttcagtaga aaattcttca ttttttttaa
     1861 aacccagctt ttcataaaaa gcaacggaac gattgacatc agcaacagga aaattaacaa
     1921 aaaccatact attcatttgt ttcgtctcct ttctgttttg ttcgtctatc atctcttatt
     1981 taggaaacgc tgtcaaaaat ttagattcat atatagaaac caaaataaaa atttgcatca
     2041 tcaaaaaagt gaagatgtga tagaatgagg actggttcac tttcacttaa ctaatagatg
     2101 gaatttcttt ataataaaaa agtgaattta tattgaaaaa gaaaaaaaca agtaaagccg
     2161 gggaatattc cggtatgaaa ggaatggaga gaacaatgac catccaatgg tttccgggac
     2221 atatggcaaa agcccgacga gaagtttctg aaaaaatgaa atatgtggac atcgtatttg
     2281 aattggtaga cgcacgatta cctttatctt ctcgtaatcc aatgctagat cagatcattc
     2341 agcaaaaacc tcgccttgtt ttattgaata aagcggatct ggcagatcca caacagacaa
     2401 agctgtggca agaatatttt cgtaaacaag gccatcttgc attagctatc acggcacaag
     2461 aaagtaaagg aattaaaggt ctagtacctg cggctaaaga agcattgaaa gaaaaacggg
     2521 caagagatgc tgctcggggg atcaagcctc gcgctattcg ggcaatggtc ttaggtattc
     2581 caaatgttgg gaaatctacg ttgatgaatc gactagtagg caagaaaatt gcacagacag
     2641 gaaataagcc tggcgtaaca aaaggtcagc agtggcttcg tttaggtaat gagttggaat
     2701 tgttagacac accagggatt ttatggccca aatttgaaga tgaagaaatc ggcaaaaaac
     2761 tagcgctgac aggcgcgatc aaagaccagc ttgtccattt agatgattta gcgatttacg
     2821 gattagactt ttttgcccga tattattcag gaagaatcag cgatcgttat ggtttatcaa
     2881 tcgagcaaga agaaaaattg gcaccggaat tactgatgga tattacagca aaacgtgggt
     2941 ataaagaaga ctatgatcgt ggcagcgaat tagtcgtgca tgatattcgt caaggaaaac
     3001 ttggacgata cacactggat cgttgcgaag aaatggagca aaaaagtgat gactgaatcc
     3061 atccaaagta tcaaagaacg attgaaaagc atacagagaa tagatgatcc tacactagaa
     3121 tcattaagaa atgatccacg caaaggcgtg cagcaagcac tggcttcttt tgaacgacga
     3181 ttgaaaaagg aagcattaat taaggaaaaa tatgaagaaa tgttttgctt tgaaaagcaa
     3241 gggtacgcac aaggtcacca atggattgcc ggcattgatg aagtaggaag aggaccatta
     3301 gctggacctg tcgtggcagc tgcagttatc ttgccgaaaa atgagatcat attagggctg
     3361 aatgactcca aacaattatc agagaaaaag cgagaacttc tttatcaaca agttcaagaa
     3421 aaagccctgg caatcgggat cgggatcgtt gatcagcaga caatcgatga tgtgaatatt
     3481 tatgaagcaa gtaaattagc gatgatccaa gcagtagaaa aattgtccat tgtaccaagc
     3541 tatttgttga tcgatgcgat gaccttggat ttgcctattc agcaggaaaa aataatcaaa
     3601 ggggatgcac gatctgtgtc gattgctgct gcaagtatca ttgctaaagt atatcgagac
     3661 caattgatga aagaatatga cgtgctttat cctggatatg gtttctcgca taatgcaggt
     3721 tatggaacaa aggaacactt agaaggactt gaacgacaag gaattacgcc aatccatcgt
     3781 aaaagttttg cacctgtaaa aaaatattta caataaaaaa cagccgcttt tacgtatctt
     3841 attagaaaga gagacgaaag gcggttttat gatgtatcaa atagaagaaa atttattgaa
     3901 acttgcttat acaaaaggaa tcagtagtca aggaaaatgg gcacttgcta actggatgat
     3961 gcagtatcct tataaagaaa tcggatttga agacatcatt aagataggta aaataaccaa
     4021 acatcgggag ctgtttcgca aaagttggca ggaaattcac gctaactggg gcaaaatcca
     4081 atcgaaacag tcgtttatca cttgctttga tcccaaatat ccccctcagc tgcttcactt
     4141 gacctatccg ccaattgtac tgttttataa tggtgatcta tctttacttt cttgtaacat
     4201 gttaagcgtt gttggtggaa gacacacttc tgctttagcc aaaaaaacag tttattattt
     4261 actaaaacca gtgatagaag ctggatatgt gatcgtcagt ggctgcgcga aagggataga
     4321 tacatacggt caccaagcag caatctctca tggtggacgg acaatcgcag tcattggaac
     4381 agggatagag caagtctatc ctgctgaaaa tcgtgacttg caagaggtga ttgcaaatga
     4441 tcatcttctt ttgagtgaat atcctcctca atccggcccg aaaaagcatt gttttccaat
     4501 gagaaatcgg attatcgcag ctttgtctaa aggaacatgt gtctttgaag ccaagaaaaa
     4561 aagcggctct ctgataacag cacagcaagc attggatctt ggaagaacag ttttttctgt
     4621 tccaggaaat attttaacag aacattctac cggatgtcat catttgatcc aagacggtgc
     4681 agtttgcacg gtttcgggtg aagatatctt ggcagaattt aaagaataac accatacttt
     4741 tcatttgctt ttcttgacaa acgttttctg aatggatatg attagctacg atttgtatga
     4801 atcctctttt aatagtagga acacgacaat ggaaaggagt ccattcagta atggcgtata
     4861 aatatttagt gattgtagaa tcacctgcaa aagccaagac aattgagaaa tatcttggac
     4921 gaaactataa agtaatggcc agtgttggcc atataagaga tttaccgaaa agtaaaatgg
     4981 gtatcgattt tgaaaacaat tatgaacccc attatatttc tatacgcgga aaaggcgatg
     5041 tcatcaaaag cctgaaagcc gcagcaaaaa aagctcaaaa agtttacttg gcaagtgacc
     5101 cggatagaga aggagaagcg attgcttggc atttagcgta cctacttggg ttggatctga
     5161 aagaaaaaaa tcgggtggtc ttcaatgaaa tcacaaaaga cgcagtcaaa gcagctttta
     5221 aggaaccaag aacgatcgat gtagatttag tagatgcaca gcaagctcgt cgtaccttag
     5281 acagaatcgt tggttattcg atcagtccta ttctctggcg taaggtcaag aaagggttaa
     5341 gtgcaggacg tgtccaatct gtcgctttaa aaattattat tgaccgtgaa aaagagatcc
     5401 gagaatttgt tccagaagaa tattggagca tcgacggtaa ttttaaaaaa gctcgcaaga
     5461 aattcaaagc aaatttctgg ggaatcgacg gtaagaaaaa gaaattacca gatgcacaaa
     5521 gtgtaaaaga agtcactgct agaattgatg gaacagactt cgaagttaaa aaagtagaaa
     5581 agaaagagcg caaaagaaac cctgcgaatc ctttcactac aagtagcctg caacaagaag
     5641 ctgcccgtaa gttaaacttt cgaacaagaa aaaccatgat ggtagcacag caattatatg
     5701 aaggaatcac tttgggtaaa caaggaacag tcggcttgat tacatacatg cgtactgatt
     5761 ctacccggat tgccgataca gcaaaagcag aggccgcagc atttattgaa gatacgtatg
     5821 gaaaagaatt ttctagacat gatcaccgaa agatcaaaaa tgctcaaggc gcacaagatg
     5881 cccatgaagc tgtgcgtcca acgagtgtgc ttcgtacacc agatgaactc aaacaatatt
     5941 tagataaaga tcaattgaaa ctttatacat tgatctggtc acgttttgtg gctagtcaga
     6001 tgacaccagc tattttagat acaatgaaag tgacattaga acaaaatggt gtcatcttca
     6061 ttgcgaacgg ttcaaaaatc aaattcaaag gattcatgca agtgtatgtg gaaggtagag
     6121 atgacggcaa agaagagaaa gaaaatattc ttccagaact tgaagaaggc gatgtagtcc
     6181 aatctgttga tattgaacca aaacaacatt ttactcaacc accagcacgt tttagtgagg
     6241 ctacgttgat tcgtgcacta gaagaaaacg gggtagggcg accttccact tatgcaccaa
     6301 cacttgatac gatccaaaga cgctactatg taaaattgac acaaaaaaga tttgagccga
     6361 ctgaactagg agaaattgtc aattcattaa tctgtgaatt cttccctcaa atcgtcgata
     6421 tccatttcac agcagaaatg gaaggagatt tggataaaat tgaagaaggc acggaagcgt
     6481 gggtcaaagt ggttgatcgt ttctataaac cgtttgaaaa agaactgacg aatgcggaag
     6541 aaaaaatcga aaaaattcaa atcaaagatg aaccagcagg attcgattgt gatgtctgtg
     6601 gacatccgat ggtgataaaa ttagggaaat atgggaagtt ttatgcttgc agtaattttc
     6661 ctgattgtcg gaatacgaaa ccaatcgtaa aagaaatcgg tgtgacttgc ccagtatgtc
     6721 atgaaggaca agtcattgaa cgaaaatcta agaaaaacag actattctat ggttgttccc
     6781 gtttcccaga atgtgaattc acttcttggg ataaaccagt gggcagaaat tgtccaaaat
     6841 gtgacggata tctggtcgag aagaaagtca aaggcggtaa acaagtagtc tgtgtaaatg
     6901 gagattacga agaagacatc caaaaataat agaaaagagg ttctttcatt gaaaatggta
     6961 aatgttgttg gagcagggct tgccggaagt gaagctgctt ggcagatcgc acaagcagga
     7021 gtccctgtcc gtttatatga aatgcgtcca gtcaaatcga ctgaggcgca tcacacaagt
     7081 aattttgctg aactagtatg ttccaattca ttaagaggaa acagtttggc taatgcagta
     7141 gggttattga aagaagaaat gcgccgattg aattcggtag taatcacttc ggcagatgaa
     7201 acagcagtgc cagctggtgg tgccttagct gttgaccgcg attctttttc tgaaacaatt
     7261 acccgaaaag tcaaagagca tccattagta acagtgataa acgaagaaat tactgaaata
     7321 ccggaaggaa tcaccgtagt agcaacagga cctttgactt ctcatccatt agcagaaagc
     7381 atcaaagcat tcaatggttc agatggtttt tatttctatg atgcagctgc accaatcgta
     7441 gacaaagcta cgatcgatat ggataaagta tatttgaagt cccgctatga caaaggagaa
     7501 gcagcttact tgaactgtcc gatgacagaa gaggaattca aagctttcca tgaggcacta
     7561 atcaatgccg aagtggcaga acttaaatct tttgagaaag aaaaatattt tgaaggatgt
     7621 atgccaattg aagtaatggc acagcgagga ttcaaaacga tgcttttcgg acctatgaaa
     7681 ccagttggat tggaagatcc taaaactggc aaacgtcctt atgctgttat ccagctgcgt
     7741 caagacaatg ctgcagcttc tttatacaac atcgtgggct ttcagactca cttgaaatgg
     7801 ggcgagcaaa aacgtgtttt ccggatgatt cctgggttag aaaatgctga attcgttcgc
     7861 tatggtgtga tgcaccgaaa cagctttatg aattcgccag aattgctgaa accgacttac
     7921 caatcaacga aacgcgaaga tctgttcttt gcaggtcaga tgactggtgt agaaggctat
     7981 gttgaaagtg cagcgagcgg actacttgct ggaatcaacg ccgcacgctt agcaaaagaa
     8041 atggaaccag tcgagtttcc acaagaaaca gctattggaa gtatggctta ctacattact
     8101 catgcggaag gaaaacattt ccaaccgatg aatgccaact atggtctgtt tcctgaactg
     8161 ccagagcgca ttcgagacaa aaaagcaaga tatgaagcaa ttgcacaacg agctttaaaa
     8221 gctaatgagg caataaaaac agaattagaa aaaactgcaa tcccaaatga gtaaggaaac
     8281 tccatgaaga gggtgagacg gatagtctcg ctcttttt
//
LOCUS       DAPCMT010000120         8254 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPCMT010000120 DAPCMT010000000
VERSION     DAPCMT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.122"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(679..2310)
                     /locus_tag="RD743_002253"
     CDS             complement(679..2310)
                     /locus_tag="RD743_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDU0564051.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2324..3997)
                     /locus_tag="RD743_002254"
     CDS             complement(2324..3997)
                     /locus_tag="RD743_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDU0564052.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4010..4327)
                     /locus_tag="RD743_002255"
     CDS             complement(4010..4327)
                     /locus_tag="RD743_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDU0564053.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4352..5752)
                     /gene="lacG"
                     /locus_tag="RD743_002256"
     CDS             complement(4352..5752)
                     /gene="lacG"
                     /locus_tag="RD743_002256"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDU0564054.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5989..6774
                     /locus_tag="RD743_002257"
     CDS             5989..6774
                     /locus_tag="RD743_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDU0564055.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6777..7469
                     /locus_tag="RD743_002258"
     CDS             6777..7469
                     /locus_tag="RD743_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDU0564056.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7743..8114)
                     /locus_tag="RD743_002259"
     CDS             complement(7743..8114)
                     /locus_tag="RD743_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDU0564057.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2521 a         1814 c         1422 g         2497 t
ORIGIN      
        1 taaccaaata ttggattcag aatctagttt tcatatataa acagagtatg attaggtgca
       61 aaaattgcac ttttattgca cttttatttt tatttatagt gttttactgt tctgtatatt
      121 gttttaactc ccttgatagt atcgcattct gttttataca ctttacgttg aaaacagagt
      181 gggaataaag agccaacata atatggaggg ggaatcccta taaatcaaca ttcttcaata
      241 gctaaaatgc ttgattttca ttaaaaataa gatggttccc taccaaaaga gactataata
      301 taagttaatt tgtttcattc ttatcataaa aagaagttct cgaaaaacga actgttttcc
      361 gagaactttt ttctatccat ctaagtgctc aaatcatatt cacgacggaa ttctaaggtt
      421 accctttctt aaacaaactt tatgttatct gcacaacttt ttgcaatttt ttcattgctt
      481 cttacgaaat cgttgaactc tctttagcat aatacaaagt gtagatgaag tctggtgctt
      541 tactcgtttt ggtaaaaatg actggaaatc gtttctggtt atttttggca aaggcgaggt
      601 gcaggttcga ttcctgcctt ctacgctaac gatgtggctg aagggaacct ccgatcttga
      661 atcgaaacga tccgcagtct attgtttcac caatgaaaca aaatcaacgt tcgtccaagc
      721 atttcctgaa gccttgctat aaaccccaat ttcacaaaca cctgttgtaa ttggaatgtc
      781 tttgattgta atttttgtcc attcacttgt tttaacaacc gcagtattta gttccgctcc
      841 accataattt ttgatatata actgattagt atcaaactct cccccagaca ttacataggc
      901 actcaatgtg taagttcctt tgcttatgcc actaacattt tgataagctg atgcctcgta
      961 tgcttgatcc tgccaaaaag ttagtttgta gtccccaaaa aaagtgcccg cttccgtttt
     1021 gatcgtaccc ttagcggttc cttgttgaaa ccaggtttgc caattggaag gactggtcgt
     1081 tggtccatcg ctttcgaaac ttgcattttt aactagattt atttgcgccc ctgcttgttt
     1141 aaacgcacgc aagcttggca aggcgtttcc agcaaaatca aacatcgctt ggttgtccca
     1201 ttcgtttccc atagtggatt gaacactgag ataattcata cctgcttgtg ttgcccaact
     1261 cacatttccg ttgtaccaaa gaggttccca atagaagaag cccgccccgc gattattcgg
     1321 tactgcctga atccgatcgg caagatccag caaatactct gcttgtcctg tcggtgttgg
     1381 ttcatatccc cccgcagctg cttcggcttc accaaaagca tttggcatag tatcggcatt
     1441 cgctgttgta tgcccataag ctgtttcaac tacgatcact tctttaccat accgttggct
     1501 gatcgcattc atattggttt gaagttcatt cattgaaccg tgccaatacg gataatacga
     1561 cactccaatt aaatcaaatg acacacctcg actcgtgatt tcatcaaacc accaagtaaa
     1621 tgctcctgta tcgccaccat cagctaagtg gagcattact ggagttgttt taggttgagt
     1681 gtctcgaaca gcttggatac cagacttcaa aaaagcagcc aatcgatcaa attctccccc
     1741 gccttctccc caagacttgc cataaggcca gagcattccg ctattcagtt cattgccgat
     1801 ctgaaccata tcaggataaa cgtccagctg cttcatttcc gacagaacgt tctttgtata
     1861 gtcgtacaat gccgtattca tttcttcaaa ggtcagacct tgccatgctt taggaagatt
     1921 ctgtttccca ggatcaaccc aaaaatcact gtagtgaaaa tccagcagca ccttaagtcc
     1981 cagatttttt gctcgttttg caaggtcaat cgtcgtttcc agatcatttg tccctgctcc
     2041 ataactttct ccattactat tataaggatc ctgccacaaa cgcagtcgaa catagttgac
     2101 accattattt tttaaaatct ctaaggcatc ttttgaaata tcgtcttcat aatattttgc
     2161 accagatttt tccatatctg acaggatcga aatatctgct ccgcgaataa attcgctggc
     2221 agatgcttca acagtttttg aaacagcccc caaagcccct aacacagctg caaataaaac
     2281 agctttacgc aaccaaattc ccattttcat tatggctcct cctttattga aattggttca
     2341 gaataaactg aacagctttt tcaggatctc tcgttaatcc gatatattct gctccttttg
     2401 tcgcaaccag cttgattcct aagcgatcgg tgtcttcttt gatatcatca tagtaagaat
     2461 tgacttgcgg tgctaagacc accatatcaa aatcctgcag catttcgtaa tgagaaccat
     2521 aagcacccgc cattgcactg atcggtgctt tcgattcaga tgcacctttt tttaaagcat
     2581 tagctaacat cgcacttgtc cctgcgccag cacaaagtac caacacattg atttctttat
     2641 ttgcatcgct gataacttga ctcgctgcca caggtacttc ctcacgtaca tcggcagaag
     2701 caccttcctc ggttgcgatc acctctgctt tttgtgcttc tttctctact aagacagcat
     2761 cgtatgcttt gcaaaatggt agatagatca ggaagtcaac gacgattaga accgctgcta
     2821 gaacaaaagc taagataccc atgcccgttc ctagaaccaa accgatcggt gctggagttg
     2881 cccaaggcaa aacgtacata aagctgttca tgcccaatac atcgacaaaa aacttgaaca
     2941 ggcaaacgtt tacaacaggt gttaacaaaa atggaataaa aaaataaggg ttcaaaatca
     3001 tgggtgccgc aaatagcaat ggttcgttga cagcgaaact tactggaata aaggaagctt
     3061 tcccaactgc cttcaactgc ttagacttag caaagagtag gaagataaaa ggaacaacca
     3121 atgttgcccc agttcctccc atagttccta caaaattccc taaacccacc gtcagtactt
     3181 tagaagcttg ttcaccggaa ttgaacaatt gcagattggt ttccacattg gcatagataa
     3241 ttgccgcaat tgccggctcc acgatcgacg gtccatgaac accgacaaac caaaacagcg
     3301 ccatcgctcc ccagataata gcgattccta agtacccgtc agctgctgta aatagcggct
     3361 gcagcaccgt aatgattgct tctgcaaatg agatttggaa gatttgtcga atgcctaaat
     3421 caattaaaac ggccgcaaat actgaaaaag agaaaggaaa aatatctcgg aaggtttgcg
     3481 aaatcgttcc aggaacttct ttaggcattt taatggtaat atctttcagc acacaaaact
     3541 tatataaatt aactgttaaa aatgcagcga caaaggaagc cagtagtccc tttgtcccca
     3601 tatattccgt tgaaaatcca ccttcaatcg gagagacgct taacaacagg aaacttacca
     3661 cagaggccag catcacagag gtggtgttga ttactttgtt tttcggtaag cgacgattca
     3721 tcgaatcagc caagcagcga gcagtggttg ctgaaacgag taaccctaca acacccatcg
     3781 aataattata gactttccat agccatgtgg agacagattc aggccattgg taaccaaaaa
     3841 tctcaggaac cgcagcaatc aaaatgaaaa tgcttgaaaa taaaatagct ggcatcgctg
     3901 ttaaaaatcc gtcacgaacg gctcctaaat aactattacg tgagattttt tcaaaaaatg
     3961 gtttcccttt ttcgatttgt ttaatgattc catccatagc gttcttctcc tatccttgat
     4021 tctgtctgta cagtacaata aagtcttgaa tcagatctct cagcaataaa gtggtcatta
     4081 aatgatcctg cccatggata aaaatgaatc ccatctccat ctctttccca gatgcttctt
     4141 ccgctaaaat ttttgtttgc gaattatgtg caagggttaa attttcgtcg gcttctttca
     4201 acgccgaatc aacatgttca aagtttcctt gtcttacttc tttcaataat ttcaataatg
     4261 tactgcgagc gtcgcctgaa tatgcaacga tctcaaaacc aagcatttgt aattcctctt
     4321 tattcatctt ttcttcctcc attatcactt attaaataat cgttttggtt tcactgacca
     4381 acttgtacca gtaagctgat tcttttgggt aacgcttctg ggtttcaaaa tcaacataaa
     4441 acaagccata ccttttgtta tacccatttg tccatgagaa aagatccata agcgaccaca
     4501 aaaaataccc cttcacattt accccggctg ttatcgcctt gcttaaggat tccaaataga
     4561 ctctcaaata gtcgattctc ggctgatcca taataatgcc atcttcaaac tgatctttat
     4621 accccatccc gttctcagtg atatagattt tgttgtaatg tgggtaatcg cttttaattc
     4681 gcagcaacaa gtcatataga ccttctggat aaatcagcca gtcccaatca gttcgaggaa
     4741 ttccttcttt ataaatacgt tctccgattc ccttcacttt ataaacagag gtcccttttt
     4801 cacccgtacc gttatgatgg attgcatttt ctccatcgta agctttgaca aaatggcatt
     4861 ggtagtgatt gatccctaag tagtcattac gcgtggatgc ttttttcaat tcaacgaaat
     4921 cttcttcagg aaaatgatag ctggcctgat ttgcttcaca tatctcatca agagcagtca
     4981 aggtttcagt tgaataatag cctaaatagg tagcatctaa taagaaacgg atcgacaatg
     5041 catcgtctaa aaaggcagca tgcttatctt ccggcgcgtc tgttgccgca tatttcgtct
     5101 ctaatgagtg aactacacca atttcacccg gaagttcgtt ttctttgaaa tagttcacta
     5161 cacgggcatg agcaaccatc atattatgaa gacaggcgac gatctttgta aaatcatatt
     5221 tgattcctgg tgggaaaaca cctaacaaat attgattggt tgcaactgga tagatttcat
     5281 taaaggtact ccacaccttg acttctttga attcatgaaa acaaaaaatg gcataggaaa
     5341 caaatgcttc tattgtttct cgattcaaaa aatctccatg gtcaaacaaa cgtttagggg
     5401 tatcaaaatg atgcaacgtc acaaacggcg ttacgtttct tttcttacat tcttcgaaca
     5461 cacgatgata aaaagcgatt ccttctggat ttggtttccc agctccatca ggaaaaatgc
     5521 ggctccaagc aatggataat cttagaccat ttacaccaaa acgttcacac aattcaatgt
     5581 cctttgcata ttgatgataa aaatcacttg ccggatcggg actgaatcgc ccttgttcct
     5641 ctaaaaagtc atcccaagca acagcgcctt tgccaccttc cttagtggct ccttcaacct
     5701 gataggctgc tgtggctccg ccaaaaataa aatcttctgg taatttaagc atgtacatct
     5761 ctcctcaaat ctataatcag ttttcgctct ttctaactga ttgcattgta aacgcatcca
     5821 aaacatttgt caacaaaaac aaacacaaat aatcataaaa accagaaaac agcaataaaa
     5881 tctattgtaa caaacataaa aaccacccta aatgtttact tttgttcgaa ttagattgac
     5941 tgttgtccgt ttatatgata gattatcctt aaattagagg tgaagacaat gttgaaaaaa
     6001 gaaagattgc ttactatcgt agaaatggtc aataaaaaag gaatccttac cgttaatgaa
     6061 atcatcaatc aattagatgt ttctgatatg accgttcgca gagatttaga tgaattagaa
     6121 aaatcgggca agctgctgcg tgtccatgga ggggctcaaa gtctttccta cactttagat
     6181 caagaacttt ctcacactga aaaagccact ctgcagattc aagaaaaaaa agaaattgct
     6241 gccacagcag cctccttgat tcacgaggga gaaacgattt tcttaggacc aggtacaaca
     6301 atcgaactat tggcgacgca tttactgaac aagaaaattc ggatcattac aaataattat
     6361 ccagtttttg atattcttgc tcaagcagat ggaccagaaa ttctactgat tggcggtgat
     6421 ttccggaaaa ataccggcgc atttgtcgga ccgatcacca acgacaattt aagaagacta
     6481 aagtttacaa aagcatttat cagttcaaat ggcgttcaca acgaagcgat ctctacttac
     6541 agtaccgaag aaggagaagc acagcagatc gctctaaata attcccgtaa gaaatatcta
     6601 ctcgttgata ataaaaaatt caatcgagat gatttttatg tgttttataa tttgcatgat
     6661 ttcgatctgc tcattacgga tcgccagatc tcgcgggagg ttcaagagca ctatgcacaa
     6721 tatgtcgatc tgcaaattgc tgattcaacc gaaaatacca ctataggagc gtaactatga
     6781 aagggattat atttgatttc aacggaacaa tgtttttgga ctctcatctg catgaagctg
     6841 cttggctgca tatgatacat gatcatacaa aagatggttt atcagacgag gatattttaa
     6901 aaaatattca tggtcggacg aatacggaga ttttgaaaca tttcatttca gagcatttga
     6961 ccgcctctga gatcgccgta ttgtcagaag aaaaagaaag ctattatcgt atgctttgct
     7021 tgcaaaatga aaacgagctt cagttgacaa aagggcttga agcgactctt gaccagctga
     7081 ctgaccaaca gattccatta acgattgcca cggctactgt caaagagaat gttgcttttt
     7141 attttgatat tttcgattta gcccgctggt ttgactggga caagattgtc tatgacgacg
     7201 gctctttccc tgggaaaccg caaccggata tttttcttaa agcggcagac aaactgagct
     7261 tagctccggc tgattgtctt gttatcgaag atgcttactc aggattactt gctgccaaac
     7321 gtgcggggat cggaacaatt attgcaattg atccattcgg taaaaacaaa acagtatttg
     7381 aacaggcaca gctaaataaa gatggaacca tcaaagattt tactggtttt tggacagagc
     7441 acctgtctgc cacatccgtt aattcttaaa cgattcaact aactaccaaa gaaaagcaac
     7501 tgaaatgcac acctcctttt ataaaaaact gccagtgaaa cgacatgaaa gatacactgc
     7561 tatatcctta gtgaaatata gcagtgtatc tttgactttt tcattctttt aaccatatga
     7621 cgcgttcatc tctatctata attaacatta aaaatagtgt tttcacatta tttctcaaac
     7681 caatcttaaa aatcatcagg agtacttttt tgatatactt ttattaatgc tgggcgtaat
     7741 ggttaaaaga tttccactag ttcctctcca ccaagctgag ttgaaaaatg cttatctgta
     7801 aacaacatag acaaagagga aataaacgtt tccgtatctt tctctgtccc aaggatatta
     7861 attaattttc ctactaggta agtatcatta aacttaagat ctgccttcac atcaaaagtt
     7921 ttgataaaag cttttatcat gcgtacaatt ttgtctttat cgccataatt gatttcacca
     7981 ctcatatcaa tatagtcact aatccctgaa gaaataatgg caactgcttc ttctttcgcc
     8041 ttatcagata attcatttaa tgactgggat tgattgatga cttcttgagt tttatctgtt
     8101 acgtaatctc tcattatcct atcggcatac tccgcaaagt aatcttgaaa aaattcttct
     8161 acagggtaac caacttcgtt ttcccaacca ggagaaattt cattatcagt acttattaaa
     8221 tctattaaaa accaacccgt tctctgagtg ttga
//
LOCUS       DAPCMT010000121         8208 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPCMT010000121 DAPCMT010000000
VERSION     DAPCMT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.123"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..671)
                     /locus_tag="RD743_002260"
     CDS             complement(<1..671)
                     /locus_tag="RD743_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0564058.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKH"
     gene            732..1472
                     /locus_tag="RD743_002261"
     CDS             732..1472
                     /locus_tag="RD743_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0564059.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(1558..2316)
                     /locus_tag="RD743_002262"
     CDS             complement(1558..2316)
                     /locus_tag="RD743_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDU0564060.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(2313..3341)
                     /locus_tag="RD743_002263"
                     /pseudo
     CDS             complement(2313..3341)
                     /locus_tag="RD743_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(3361..6528)
                     /locus_tag="RD743_002264"
     CDS             complement(3361..6528)
                     /locus_tag="RD743_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564061.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(6752..7636)
                     /locus_tag="RD743_002265"
     CDS             complement(6752..7636)
                     /locus_tag="RD743_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDU0564062.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(7731..8168)
                     /locus_tag="RD743_002266"
     CDS             complement(7731..8168)
                     /locus_tag="RD743_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564063.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT         2357 a         1732 c         1412 g         2707 t
ORIGIN      
        1 tcatgtttga attcctctag atgatggtaa tattcttcat ttatttgata cagttgaagc
       61 gattcctgat gttgtctttc ttttacttgg aaactgatca acataatgga aaaaaacaaa
      121 gaaaaaaaac tgatgactat cgacaaaaga gatagaagca gataattaga tgagcggaag
      181 tgaacacttc ccaataccaa gtaaatcacg ccccagctta ataaaataca aatacctcca
      241 aaatagtgtt cagttggata agtaaaaagc aagttgagaa atctcgtctg tttgtctata
      301 ctttttacta atagatagaa aataaatgcg ataagtagtg caaaaaaact tgctgttcca
      361 agatgtctct ttcctaaata atccatcaga tcaaaaacca gaaaaaagct gagtccagtg
      421 atagcaaaaa aaagattact gatcaaacag ctgataaaca ggtttttcgt agtaaaataa
      481 aaaagaaaca tcaataataa gccaactagg taaggaaagc tcactttagg gataggaagt
      541 aaaagtgtca ccaccatcaa agctatacaa ccaaagctgt tttttactag cagttgtttc
      601 gaaaagaata aatagatacc taaatctaaa atcaaagtaa aggtcaaata aagtgtcact
      661 gttgcgacca tcttattcct ccattgtttt gtttcaaaat gtacacatat ttttttaata
      721 ggagcctgca tatgaaaaag tattttatca tagaagataa ccagtttcac agcgaatgga
      781 tcatcagaga attggtcaat catcatatcg agaatatcgt gcagctcact tctatctctc
      841 aaatcaatca gttgatccag ttgggcacta ttgataatca cgatgttttc tttatcgata
      901 tcgacttgaa gcaatcactc acaggtattg atgtcgccga acaaatacgt aaaaaaaatg
      961 cacatagtaa tattgttttt ttcaccgctt atacgaattt tgcagtagag atcatcaatc
     1021 ggaaaatcct tccgattgga tatatcagaa aagacaggaa tttatctgaa caaattcgtc
     1081 aagtgctaga acagatcaat tggaaagaac agcagatcgt aaatgtcgat aaaatcttta
     1141 ttgatcaaaa aggtggaagt ctcgtcctgt atcctcggga aattttatat attgaaagta
     1201 tcaaaggttt gaaaaaccaa gttatggtca aaacaactac agaagataaa ttagtgaata
     1261 cttctttaaa acaattgaag acagagttag aaaagtatgc ttacttttta cttttaaaat
     1321 cttactgtat caacttatcg caattgcaga aaattgattc tgctcattat gtgttagaat
     1381 ttttcaacgg tgactcctta ctcgttggtc gaaagatctt tgaaaagaca aaggaacgtt
     1441 tccatgactt ccaaaaaact gcttcgagtt gatgaataag agctgagacg taagcatagc
     1501 agatttcagg gagtgggcct tgaatcggct ccactccctg aaatcgttga tatgatttta
     1561 cgcttcctct tctgtaggag catctgttaa cgaccatgtg aatgttgaaa cgtagttgtc
     1621 tacagcataa ttggatacag gaacaaataa ttccactgat tgtctccctt gttccacatt
     1681 ctctcctaat gaataaatcc atgtgccaat cccttcattt gctcctgcag ttactagatc
     1741 tactgcctga tctgctggga tttcgattcc ttcagcgcca gttccagaaa cggtagatgg
     1801 cattccactg ctattagttg cgctatttcg aatcacggcg tttttgaatt tcacctttga
     1861 accagttagt ttattcgtat cagatgttgt accaataaat tctgttcgtt tgactgataa
     1921 tttccaaccg gcataatttc cgcttttatc cgttacttgt acgaaattct gtacttgtgt
     1981 ttgtgctcca gtactgtccg ttccgctttg taatgcagca tatactgttt ttgctgtacc
     2041 taacttgatt gttccaaaat caagatctgg agcttggtca atcgtcagta atccggatgt
     2101 tccctgattt ggttcgtcag gtgtaaaagg agtatcagga tcatttggat caacaggagg
     2161 aactggaaca acgtcttcag gatttgcagc atctggtttg gtcacagaaa tcgttccttc
     2221 tgtagtggct gagctcttat tctcagcaaa aacactttgt gtacataata atagacttcc
     2281 agctagaacg gtacttaatg caattgtttt tttcatttct ttttccttct ttcttttaaa
     2341 ataaataatc cagcaaataa gccaataaga atgactccaa caagtatcag ccatcgccag
     2401 tcaaatgttt ctggctgatt ttttaaatca atatcctttt cattcaattg gtccgctgca
     2461 tcatcagtta tttcaaaatc ggtatggaga ttccagcgtt tttctttcca agcacccttc
     2521 acaaccgctt gataatgtcc cggagctaat ttttttcctt ctaatgggac aagtaaatca
     2581 aaatttgtat tgggtgctac atctattttt tcttttttta ttgaaaggta aggctctttt
     2641 gtttcttttt tgtagatttc aaccgatgta gtcaagccga acatataaga agcatttgga
     2701 ttttgaagat tcgccttaat cacatttctt ccatcgcttt gatccgcata agcttttttt
     2761 aatttcaaat ctggggaaga ctctttttcg ctatttcaaa tcaagattgc tttcacttga
     2821 gaaaactgat tgacgacacc aactgtactt ttgttagaaa cgttctcttt tttttcttga
     2881 aacgtgatcc ctcctgcgac cacaccagcc agctcttttt ctggcgcttt cagtgaaagt
     2941 gtctcagtga ccgcactttg aggaggaaca gttatttctc ccactgtttt tgctaagttt
     3001 ttcatatcat aacttaatgt ttcatcaaat ttactttttg attctccata ttcgatcaca
     3061 ccgtttgaat tcgtcgtagc ggagtggatc aatgtctcaa ctgttactga tttatctgtt
     3121 cggttaaaga ccgtgtaggg gatttcctgt gttttcccag gctccatcct tacatttata
     3181 tatgttttgt tcttatcaat ctgattagcc ggtaattcaa aatctaccgc aaaattgaat
     3241 tcggaggcat ggacaggtgt ctgccaaagg atgaatccaa ggagaaggat cataagtaaa
     3301 gagcagttgt ttcgatgact tttattccat attcgattca tcaatcatcc tccttgatct
     3361 ttattctgtc ggcgcatctg ctaatgtcca tgttatctct gctgagtaat taccagctat
     3421 ctgattttct ttggttaaga caagtttaaa cgcccgaccg ttatttaaat attctgaata
     3481 atctgcttgc acattcgttg aattttgtcc tctgacaatt tccagttctt cttcattcac
     3541 taattgatac tcctctcctt tcttgaaata taggacatcc tgcatagaaa atcctgcatc
     3601 atttgtgaaa ggctgagata attttatcgt taagatccac ggatttttcg tttctcgctg
     3661 atcataaatc cataattttt cctgtatatc catttggcca aatgcttctt gtgtcaaacc
     3721 agttgctttg atctgaccaa aatcgagttt ggaaggggca agaaatgaga gaccaccttc
     3781 tactacgatc ttcgtagcag gtgcaaaagg cggggcatca tgataaacaa ctggcgtatc
     3841 acttggattg tgattcccaa tttcatcgtt ggtacttggc ttgttctgca cacccttagc
     3901 agtagcttct cctgcttggt cgtttaatac aatttctaat acatctcctg caaaaagttg
     3961 attttctggg atagaaagaa caaattcccc tgtatcatca atagttgttt ctgacaaaac
     4021 agcttgattt tttttcaatg aaaccaaggc tcctgcttca cctgtacctg tgatcgtgcg
     4081 agaagctgca ttgatcaatg tggtacttaa attagcaggt tcaggaggaa tcacatcgac
     4141 tgtcgttttg gcagagtcca gttctgtcac ttcttggcca cctcttactt ttttagcagc
     4201 aacaacttgc acttgatcct ccgtttttaa tctctcatca ttgggtactt gaacttcgaa
     4261 caagccgcct tgttcgcctt ctccccatgg gttatatcct gccccttcat tttcatctgt
     4321 ttgtccttgc gtgagagcag taatttctac agatgatttc gtcgtatctg cgtaagtaat
     4381 acgtaaagtc acttctactt cgcctgtcca agcatctctt ggctttgcct cgacaccttc
     4441 gggaacaacg acatgcccat agattttttt atctgcattc gttggcactc ttaaggattc
     4501 aatcatcggg agctgattat tggcactgat tcgattataa tttttcattc cggcacctgt
     4561 ctgagcaaat tcatttgtta aacctgcact agggcttgat acgaaattgc ctaagtcggt
     4621 cccattgatc gataagtctg tcatatcaga aaaataatcc gggtcttttt caaagttatc
     4681 gaatgttgcg cctgtttttc gccataaagc gatttctgaa tgggcgatct ttaaagaaga
     4741 atctccattt ccttggaata cccagccgcc tatgccattg gtctcgcttc caatccgttt
     4801 gttttggaaa tcaaaatagg taggagattc taagtccaca ttcaacacat ccccactgat
     4861 gatgccggtc ttatcttccc ctgttttccc gaccatgact aaataagttt tttcacctgc
     4921 cgtaaaattt aaatcaatat ctccagttgt atctacagtt gctccggaat ctgcgcgtat
     4981 atcgatattt gatttttctc cagtgactgt aaaatcagct gaacctggac tttctagaaa
     5041 accagaagcg tcataaaatt ggatcgcttg atttccttga ttgagtctgt tcacgtaaga
     5101 tttgccatca cctgtatttc ttaccctcaa tgagcctcca ttggttactg tcagcttttg
     5161 cgtaccattc tcaaaacgaa ctgcgctgga acgcccactc tgcttatcga tgtcgataac
     5221 tgatccggct ccttctacga tcaaagaaag gccttttcct acaaaacgca tcccattgtt
     5281 cccgggttga tcgtcatctc cagccatttt gaccgagatt tgtccaccac ttgatacatg
     5341 gatttgacta gaatctcctt gcaagcgaaa tccgcccgta gttgctgtat tgatggtgat
     5401 tttcccacca ttcgatacag tgactttcga tcctgaacct ctcatttgga tgattccgcg
     5461 attgttgtca ttggtaatat tgatctttgt atttaccgaa aattcgcttc ccgaaccact
     5521 gacttcaaac atgacattac caggtagcgt atcgactgcc tctccttcat cgctaacgcc
     5581 atctgcccga tcataatcaa gagagacctt tgaaccgctt aagatatgga ttcctggcgc
     5641 tgtttttcca ctaccagtct catctcctga atctagaaaa ccgtagaatt tccctagata
     5701 cggtccataa agttgactac cattgatcac attcaattga tagcttctac aaaaataggc
     5761 tttacccgga gcggcattca gtccttcctt cactggttgg acatccaaca caccggaaac
     5821 accatctaat gtgacagttg cacgaggagc gtaaatcagt cctaatttgg acccatcata
     5881 caaatcaagt gtcccggtaa attttatttc tcccgtaaaa tgatatctgc ttccccaaaa
     5941 atcggtcata taagcaatga taatggcatt tccatcgata tttggtgtca atccacctgg
     6001 atgtgcagct gttcctttaa aattctggaa ctcgatcgac tgaggtatct cattgatata
     6061 aatatggtgt gtgccgaaat tgtgagcgaa tccaccaaaa tcaataacga tatctcgttt
     6121 gatctctaat tccctgtcta aagtcaaagg ctgaatcagt cggatcttag ttactgattc
     6181 gtctgcgaga agctgcgcca gttcttctgt tgtggcagct tcgctgtacc catccgctga
     6241 aacagctctt gccagtgccg cttcactgct atcagacgtt tgttcagcta cttctgaggt
     6301 ctcacttgtc ttattttctt cttgcctttt attagtagaa gcctcacttt gagattctgt
     6361 agtttcagaa gtaatcgatt cttctgaaaa agtagtcttg tcaaaacttt gattctctga
     6421 ttgcgttgat tcgtttgttt tctctacatc ggcaaaaacg gatgtcacca tcaagagagg
     6481 gaagacaaac aaaatcgacg caatcagctt ttttacacat tgattcattc tccaccatcc
     6541 tctctgacgt tttgacaatt ttattttatc gttttaatct atttccaatt gtactttcat
     6601 atttaaaata tgtattttta tggaaaaatt tttctattag gtgtaaattt cttttttgta
     6661 caaaaaaaag gctgtgacaa aagtcttgtc acagtccctt atcccgaaca agcggtgttc
     6721 tcccgccaac tcctcggtca caacctccat tttaacaata aacctgtaaa tgtgagggca
     6781 atactttgat tgtcacaggt aatttatccc cttcatctcc atcaacattc gtcgtcaatt
     6841 cttcctcatc agctaatgac acaactaatt gctcacaagt aaggtaggct agatttttcg
     6901 tagattcatc tactcctttt aataggtctg gcaatgtttt aagcgtatcg atcaaagaag
     6961 aatcttttaa ataaaccaaa tggagcttac catcatccac ttttgcattt ggaagtaatg
     7021 tttcaaaacc gccgactgag ttagttagtc ctatcagcaa ggtactgctc tcaatttctt
     7081 ccttctttcc atcaacttct acgtgaaacg agtaggattg tgtggaagct aactgtttga
     7141 ttcctgacat gaaataagct agttttccaa atttcgtctt cttctccgca tctacatcat
     7201 tgattgattc agggatagag ccaattgcga cgacattcat aaaatagtcg tcatttattt
     7261 ttccaatatc caatggcttg actgattcaa tcgaaaaatg attgatcgct tcctcaggtt
     7321 ctagcggtat ccctaatgct cgagccaagt cattcaccgt acccaatggg aaaaatccga
     7381 aatttggccg atgttcttgt tcagcgattc cactgattcc ttcgttgact gttccatctc
     7441 cgcccatgac aaacacacta tgataccctt ccgtagccgc ttcgcgagta aagttttttg
     7501 catcccctgc tttttttgta tgcaagacga ccacttcatc aaacaccgat tccaattttg
     7561 ctatcgctaa ttgctcaaat tcctttgctt gttcaccgcc tgaactgggg tttaccacta
     7621 ataaaacttt tttcatcact ttctttccct tctgattgat ttctttaata taacgatagc
     7681 ataaagaaaa aaattaggct gtgatgaagt actgttcatg aaacattcat ttattttttg
     7741 acaagataaa acatccatac tatgaaactg aggaaaatta aaacaatgat ataggaaata
     7801 aaagaaaatg ataggcattg tatacctaat aaagtaaaaa tcttaggtgg cagataaaaa
     7861 cacaaaaata gcaagagaat gtaatagcgt ttcttccact ttttgttatc agaccttata
     7921 gcgatcagag gaataccaaa tgcataactg aaaaactctt gatgtactgt taatatgtca
     7981 cggccttgaa taataaatgc gacaatcccc gctataatta gtgtgcctaa caccaaaacg
     8041 gaaaattgat gaatatattt tatatacaaa tgatccattg tacttcctgc ctttttattt
     8101 gtgagaaaaa caaatccgta tacgtgaata acttctagaa ataaaagaaa tttgcaaaac
     8161 cactccattt atctacttct acgtgcattt tttgatattg ttttcttt
//
LOCUS       DAPCMT010000122         8052 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPCMT010000122 DAPCMT010000000
VERSION     DAPCMT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.125"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(73..921)
                     /locus_tag="RD743_002267"
     CDS             complement(73..921)
                     /locus_tag="RD743_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDU0564064.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1145..3085)
                     /gene="thrS"
                     /locus_tag="RD743_002268"
     CDS             complement(1145..3085)
                     /gene="thrS"
                     /locus_tag="RD743_002268"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDU0564065.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3422..3601)
                     /locus_tag="RD743_002269"
     CDS             complement(3422..3601)
                     /locus_tag="RD743_002269"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDU0564066.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3648..5471)
                     /gene="cadA"
                     /locus_tag="RD743_002270"
     CDS             complement(3648..5471)
                     /gene="cadA"
                     /locus_tag="RD743_002270"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDU0564067.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5749..8028
                     /locus_tag="RD743_002271"
     CDS             5749..8028
                     /locus_tag="RD743_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0564068.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2361 a         1709 c         1457 g         2525 t
ORIGIN      
        1 tgatggatca tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa
       61 atagtaaggt ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt
      121 cactttttcg ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt
      181 agcaaattgg gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct
      241 agatgtttgt acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa
      301 cgcaatgatt tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact
      361 tctacctgag tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg
      421 gagcaagacg tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg
      481 cagaatatct tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc
      541 cgattccaaa cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc
      601 ttttatcgct acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt
      661 tgcttctata tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat
      721 tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc
      781 actcaacccg gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt
      841 accgataatg tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc
      901 aatacgacta attacgctca taagttcctc caccctttta atgatagtct atgtaagtat
      961 accatatcag acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc
     1021 caacagaata tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg
     1081 aatacctgtt atctattgtt cggattgctt tctatctact acttttttac aggaaattta
     1141 cctattagtt ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat
     1201 tcaaatcttc tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt
     1261 ccaattcctt gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta
     1321 tcttatagcc cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca
     1381 aacgttcttt gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg
     1441 ttgcttgtat aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag
     1501 cgacaaaacg ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat
     1561 tttccccgtc ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt
     1621 gtatagttga taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg
     1681 gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag
     1741 cggctttcag cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg
     1801 tattgtttgg atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat
     1861 atactgccac catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa
     1921 caaatgtatg accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac
     1981 ctgatttttc atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat
     2041 aagaatggat cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa
     2101 caagcatttc gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc
     2161 agtgtccaga tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt
     2221 aacctagact aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc
     2281 cttttggaag ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa
     2341 gttcttttcc tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt
     2401 ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt
     2461 ttgagtttcc acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc
     2521 cagtggatgg aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag
     2581 taatcacttc ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa
     2641 aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga
     2701 cgattttcat catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc
     2761 cattatctgt atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat
     2821 ttggatagag gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc
     2881 caagtgcatc ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac
     2941 gagtcaaatc aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt
     3001 ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac
     3061 catctggaaa agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag
     3121 atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa
     3181 aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc
     3241 cgaccgccct tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa
     3301 aagtttcagc ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca
     3361 tcaatcaatt atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa
     3421 cttatttttt ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga
     3481 catggatgtg ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca
     3541 tcatattttc taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca
     3601 taatgctctc tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt
     3661 gtcttgggta ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa
     3721 atagagaccg tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc
     3781 gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc
     3841 gctattctca tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct
     3901 ttcaagatga ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca
     3961 ataccgatat ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca
     4021 acaggtcttt gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta
     4081 cgggcatgta cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca
     4141 tccccggtga tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt
     4201 gctgcttctg ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt
     4261 gttacataga ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt
     4321 ccaatcttta cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc
     4381 agttgactaa caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag
     4441 atatggctag attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt
     4501 ccattgactg gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta
     4561 tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg
     4621 atcccgtttt tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc
     4681 agtggacaag gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac
     4741 caccaagaaa taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca
     4801 gctaaacgga caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt
     4861 tgatactggc tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt
     4921 aaagaccctg acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc
     4981 aaagaagatt catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt
     5041 tcgccaggct tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc
     5101 aattttcctt ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt
     5161 tctttacccg ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc
     5221 aatatcatca aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc
     5281 gctaatacat ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc
     5341 aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga
     5401 aaaacaaatt cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg
     5461 aaatgtgtca ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc
     5521 cagctgcctc tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct
     5581 taattgaaaa caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa
     5641 tattcagcaa ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact
     5701 atgctatact tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa
     5761 caactagaac aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg
     5821 ctggatacaa aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct
     5881 aatcacaaga ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc
     5941 caacatgagc aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg
     6001 caaacgttgt ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac
     6061 cgtgaaacag aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca
     6121 atcgttattg actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc
     6181 gcattttatg aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt
     6241 aaaatccaag aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac
     6301 tttttgctag agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg
     6361 atacaaaaag cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa
     6421 ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat
     6481 cataatcgca agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt
     6541 tctgattaca tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca
     6601 ttcaaaaatt atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa
     6661 gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc
     6721 ttagtcgatc agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt
     6781 gatatgaaaa taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa
     6841 gaaacaaatg aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg
     6901 aaaaaattag gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg
     6961 gaagaacagc tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag
     7021 gagcgttcat tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc
     7081 catcgaggga tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta
     7141 caaactgtgc cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa
     7201 catttgacaa ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta
     7261 ctattctttt tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat
     7321 atctttattg atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg
     7381 ttcccaaaag caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa
     7441 acgatcgttg gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta
     7501 acgacaagct atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa
     7561 gaacatcaag tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca
     7621 acagatgata tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc
     7681 cggtattggc gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct
     7741 caactgccag acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg
     7801 aagcgttcca ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt
     7861 ttcttgtggg atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac
     7921 accatgtgta cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct
     7981 ttgttaaaca atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa
     8041 ctgctatcat ta
//
LOCUS       DAPCMT010000123         8013 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPCMT010000123 DAPCMT010000000
VERSION     DAPCMT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.126"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            24..1178
                     /locus_tag="RD743_002272"
     CDS             24..1178
                     /locus_tag="RD743_002272"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDU0564069.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1200..1952)
                     /locus_tag="RD743_002273"
     CDS             complement(1200..1952)
                     /locus_tag="RD743_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDU0564070.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2316..4982)
                     /locus_tag="RD743_002274"
     CDS             complement(2316..4982)
                     /locus_tag="RD743_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDU0564071.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGLGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5323..6774
                     /gene="cls"
                     /locus_tag="RD743_002275"
     CDS             5323..6774
                     /gene="cls"
                     /locus_tag="RD743_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDU0564072.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7103..7918)
                     /gene="thiD"
                     /locus_tag="RD743_002276"
     CDS             complement(7103..7918)
                     /gene="thiD"
                     /locus_tag="RD743_002276"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDU0564073.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2324 a         1676 c         1442 g         2571 t
ORIGIN      
        1 atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc ttttttattc
       61 ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat cctgcaaaat
      121 accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa gacatcatta
      181 cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag ctaatcgaca
      241 tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg gtaattatcc
      301 accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag gcttgctacg
      361 gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca gaacgaaaag
      421 tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt gaagtgacgc
      481 aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta tcgattgaag
      541 acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat tatagcgaat
      601 ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc caaaaagttt
      661 ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg attgctttcc
      721 atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac caaacagacg
      781 aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac agccgacgaa
      841 tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg gaaaattcga
      901 aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt gcagtaagtg
      961 aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca caatctcatc
     1021 aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg attttcagtg
     1081 agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct ttttcgatta
     1141 caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa aagaaccctt
     1201 cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc
     1261 ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca
     1321 acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc
     1381 tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt
     1441 tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc
     1501 ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat cacgcaccat
     1561 ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct
     1621 gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg
     1681 ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt
     1741 cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatctg
     1801 ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga
     1861 taatttttta catcttcagc aatagttact tgcgattctt gctggatttc ataatattct
     1921 tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg
     1981 ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct tttgatgaat
     2041 aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt
     2101 gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca
     2161 aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca
     2221 agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga
     2281 attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca
     2341 atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta
     2401 aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc
     2461 attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag
     2521 aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc
     2581 cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg
     2641 ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa
     2701 atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta
     2761 gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg
     2821 atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg
     2881 atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta
     2941 tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc
     3001 aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg
     3061 tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga
     3121 cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa
     3181 acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc
     3241 ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct
     3301 gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt
     3361 accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc
     3421 aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct
     3481 agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat
     3541 tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg
     3601 atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg tccagtagac
     3661 attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt gtttcggatt
     3721 tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg
     3781 cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc
     3841 aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca
     3901 ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct
     3961 gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca
     4021 tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat
     4081 gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct
     4141 gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc
     4201 aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc cagacctcgt
     4261 tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc
     4321 gtattattcc cagttgaagt cactaagaat ttaccgagtc cgtaagttac gatcgttcca
     4381 ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct
     4441 ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca
     4501 tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct
     4561 accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg
     4621 ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca
     4681 ctgattatag aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc
     4741 atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt
     4801 tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct
     4861 ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc
     4921 tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc
     4981 aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aagagaccta
     5041 agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg
     5101 ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt
     5161 caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta
     5221 cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat
     5281 catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata
     5341 accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga
     5401 gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt
     5461 gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa
     5521 agtgacttga aagaattaga aaactttcag tcttttagag gagataccat tgaacattac
     5581 gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc ttcattaaat
     5641 cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg
     5701 gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca
     5761 tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa
     5821 ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa agcaaaagat
     5881 ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaagggca
     5941 ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag
     6001 attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc
     6061 ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt
     6121 cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg
     6181 aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag
     6241 cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg
     6301 attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt
     6361 gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc
     6421 aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc
     6481 aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata
     6541 atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg
     6601 aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag
     6661 ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct
     6721 aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt
     6781 tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag
     6841 tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
     6901 caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc tggacggcag
     6961 gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac
     7021 caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg atgcagggca
     7081 actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga ttgatggggc
     7141 catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag tcttttgcca
     7201 atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata catgcagata
     7261 gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt aaccaaaatg
     7321 attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca tttttagcat
     7381 gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt gctgcttgca
     7441 caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca taaaaattag
     7501 gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa gcagattctt
     7561 ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat ggtccaaaat
     7621 catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct agcatacctg
     7681 ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg tcgataaaat
     7741 ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca catgcggtca
     7801 tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct tgcatccctg
     7861 cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga tcttccatca
     7921 aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt ttctatcata
     7981 tgtttttcaa agtatccctc agtaaatata cca
//
LOCUS       DAPCMT010000124         7844 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPCMT010000124 DAPCMT010000000
VERSION     DAPCMT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.127"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            17..718
                     /locus_tag="RD743_002277"
     CDS             17..718
                     /locus_tag="RD743_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDU0564074.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1327..2343)
                     /gene="tsaD"
                     /locus_tag="RD743_002278"
     CDS             complement(1327..2343)
                     /gene="tsaD"
                     /locus_tag="RD743_002278"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDU0564075.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2340..2804)
                     /gene="rimI"
                     /locus_tag="RD743_002279"
     CDS             complement(2340..2804)
                     /gene="rimI"
                     /locus_tag="RD743_002279"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDU0564076.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2811..3353)
                     /gene="rimI"
                     /locus_tag="RD743_002280"
     CDS             complement(2811..3353)
                     /gene="rimI"
                     /locus_tag="RD743_002280"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDU0564077.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3337..4059)
                     /gene="tsaB"
                     /locus_tag="RD743_002281"
     CDS             complement(3337..4059)
                     /gene="tsaB"
                     /locus_tag="RD743_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDU0564078.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4250..5230)
                     /locus_tag="RD743_002282"
     CDS             complement(4250..5230)
                     /locus_tag="RD743_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0564079.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5254..6738)
                     /gene="galT"
                     /locus_tag="RD743_002283"
     CDS             complement(5254..6738)
                     /gene="galT"
                     /locus_tag="RD743_002283"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDU0564080.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6750..7739)
                     /gene="galE"
                     /locus_tag="RD743_002284"
     CDS             complement(6750..7739)
                     /gene="galE"
                     /locus_tag="RD743_002284"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDU0564081.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2193 a         1694 c         1393 g         2564 t
ORIGIN      
        1 aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa aactcagata
       61 tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg aacatactgt
      121 tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca gacaacggtt
      181 gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg caacttatta
      241 ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt ttggagaaga
      301 gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc ttcctgtttc
      361 ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga tacaaaaaat
      421 ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct ctagcgcctt
      481 tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac caacaaaaga
      541 tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga atagcggcac
      601 gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccgggc tgttagatta
      661 tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga gagaataacg
      721 cgaacaaaaa taagcagtga ccctgataaa tatcagggtc actgcttatt ttcctcccta
      781 aaacttctga aagatccggc agttttagat cactggacaa gactcaaaga ttttctatgt
      841 gtgtttcagt tatgtaatat tcactgtttg caaaaaatac tgttagatta actagatgaa
      901 gcgtcttgtc ctcatccgat ctagattatt cgatattcct cgacaataaa cgattactca
      961 gtgagggcaa tcagaggaac ggataatgat aaataagctg taaaaaatag caaaataaca
     1021 ataatgatta aaagtatgta aaaaataggg ctttgacaaa aacgtgtcac agccctattt
     1081 cgattaaacg gtgtgtacct gtcaactctt cggtcacaag tcataatgtt cataaacatg
     1141 aggagctgtt ttttgaaatt gttccttgtg aatcagagtt aaacgacagg cttacgaact
     1201 cttgattaaa cggcgttcaa aagcttgcat acgcggtaat aagaacagcc aggtcaaaaa
     1261 catgagaagc cgtttttcct gggctgttct tattacttga tgcagggcag ctttttcaca
     1321 acctctttat tcagataaaa ccaagcttgg atcagcattc aactgatagc tagcaaaatt
     1381 tcttttttgc atttccacat gtgctgctgc tccaatcatt gcagcattat ctccacaaag
     1441 tcgaagaggg ggaatcacta gttctacctc agggagttta gctgccaaag catcctgcaa
     1501 tccttcacgc aacccttgat tggccgctac acctccagct ataatcagtt gcttgactgg
     1561 ataattttga caagctcgaa gtgttttagt aatcaataca tcgatcacac ttgcttgaaa
     1621 gctagctgct aaatcatttt tgtctagatc ttctccacgc tgttgtgcat tatgcaccaa
     1681 attgataaat gcacttttta atccgctaaa actgaaatca taattatctt catggatcat
     1741 cgctcgcgga aaatggtaat tatcttttcc ctgatgagct agctgatcga tttcttttcc
     1801 gcttgggtaa gaaagaccta atactcttcc aactttgtca taagcttctc ctgctgcgtc
     1861 atctcttgtc tccccaatga tttcatatga tccatcttct tgcatataga ctaattctgt
     1921 atgtcctccg ctaacaagaa gtgccatcaa tggaaactgg aatggtttaa ccagacgggc
     1981 tgcatagata tgccctgcca tatggtttac aggaataagc ggcaattggt gtgcccaagc
     2041 aaaagcctta gctgcagaaa taccaatcag caacgagccg acaagtcctg gtccataagt
     2101 gacagcgact gccgataaat cttctgccga cacaccagct tctaccagtg catcttccaa
     2161 gcataaggtg atttgttcta cgtgatggcg gctagctact tcaggtacta cgccaccaaa
     2221 acgtttatga ctgtttattt gagaagcaac gatgttagat aagatctctg ttccatttct
     2281 aacgaccgct acactcgttt catcacaact tgattcaatc gccagaatca attcttcatt
     2341 catttcatcg tctccgtttt taactttctg ctcataatga tcgcatcttc cgtcggattg
     2401 cgataatatg atttccgttt acctaatata cgaaactttt ctgattcata aagttttctt
     2461 gctgcttcat tagatagacg tacttctaaa aaaacatgca gcgaatcttt tatttgttcc
     2521 gtaatcagta attgtaacaa atgccgtgca tgcccttttc cttgttctga aactgcaaca
     2581 gcaatatttg tgatctcgac ttcatctatt actttcgagt atccaataaa gccacagatc
     2641 tggttatctt cagctaaaag cagataatca gagtgaggct gttggatgtc tgccaaaaac
     2701 tgcattttcg tccaaggtga accatgtgga taagcatttt cgctaatcat ccatacgcat
     2761 tctgccaagt acgcttcatc aaaatctttt tttctgcaaa tcaactaatc tcactcattt
     2821 tctaaaagtt ttatcatatc caacgcatct tcttttgttt cagtgtaata tctttttttg
     2881 atcgttgttg cttgaaaccc caatttccga taaagtcttt gtgcatcttg attactcata
     2941 cgcacttcta atgacatcgt ctcacagcga ttcattatag caaagttctc gatttcgtca
     3001 ataagcagcg aaccgattcg tttacgctga tatgccggtc gaacggcaat attcgtgata
     3061 tggcaatcta aaccgaacgc tcttgaaccg ataaaaccaa tcattcttcc attatcaaag
     3121 atacctagat atagatgagg aagcggtgac ttcaattctg atataaaggc actttttgtc
     3181 catggcaatt cgccattata gacttctcgt tcgacatcca gcagctcttt gatatcttct
     3241 gtagtcaatt ctcttagtac gtaacgacga tcgatttgtt cgactgtttt ttctgtataa
     3301 atcattttat cttgaaaaaa gcttctggca tacgctctaa attttttcaa cataactttc
     3361 gtctccaggt gtgtgcgtct ccaaccattt ttcttctgct tctactcgtt ttaaatagcg
     3421 aggaagaaaa gcttgtttct ctgcaactgg ttcaactaaa gcacctaatt gagccacagt
     3481 tacaccactt ggaatatccc atgctggaac tgtattaatc atcgcactag gcaatatttc
     3541 ctttatttca gagaagaatt tgtgacaatc actcccgacg aaataaatag cttcattcag
     3601 cttttgtagt tgtgtgaaca aatcagaagc agaaatatgt acatcttcaa gtacagtttc
     3661 taatctattt tctttccagc ggtatgcccc agcgtataca ttttttcttc gagcatcaaa
     3721 catgggcaca ataatattat ccacaccgat gcaattagcc gcgatggttt tcagactaga
     3781 tactcctact agctctttat ctaatgtata agcaatcgtt tttgctgtcg ttacacctat
     3841 acgaagccct gtataagaac cgggaccatc agaaaccgcg attcggtcaa tgtcttttgg
     3901 acttatttga agatctgcaa acaattggtc gattgcaggc atcagtgtta cactgtggtt
     3961 gcgtttgata tttgtttgta tttgtccaag aatttgttcg tcttctacca caccgatcgc
     4021 caaagtttgg tttgcagtat ctattcctaa tgtaatcata tatttgatcc tttctgctat
     4081 gacattctcg ccttccattg tagcatactt ttttattctg aggatgaaaa taccaaaggc
     4141 aaaactagta caataataca tgattatctg ctggcgggca aattttgccc cttcctgaaa
     4201 cacaaaaaag ctgcggtatc actccatttt cccacagctt ttccatcttt tatttcgttg
     4261 attctcgaat aatcaactct gtttccacaa taatctttct tgaaaccggt gctttggaat
     4321 tacttaattg aagaatcgtc tccactgcta gctctcccat ccattcggta taaactttta
     4381 cagtagaaag cgagggcgtt acatatttcg ctacactgac atcattgaag cccatcacag
     4441 aaatatcttc tggtaccact aaaccttttt cttgaatcgc tttcagtgcg ccaatcgcta
     4501 atgcatcatt agctgcaaaa agcgcatcag gaacatgcgg attcgttttt aaatattgct
     4561 tcactgcttg atatcctgac tcgacagaaa aatctgcttc gataattgga ccttcgtcgt
     4621 agcctttttc tgccaaaact tgttgaaatg ccataattct gggatctttt agccgctgat
     4681 ggtttttctt cgtatattct gttccagcaa gcatagcgat atgttgcgct ttatgggaga
     4741 caaagtgttc aatgactttt tgaacacttg acttaaagtc gacaactaga gagtcgattc
     4801 cttgagaagt gccgtcagaa tctaccaaaa gcaaggcatt ttgggatcct ttcatttcta
     4861 ataactgagc ttcgtcgaat tttcctagtg cgatcgttcc atctgccgga agatcactaa
     4921 tggaatccca cgtttctttc aataaagtaa cgtttgcttc ttctgccttt ttttcgattc
     4981 ctaggcgaat agataaataa taaagatctt caagctcttc cgtatcgtcg taccactgga
     5041 tcaggcgaag cgtttgctta gtatttgctg catttttacg atgtttcgta taatttaagc
     5101 gttctgctgc ttcaaaaatt ttttgctttg tggctaagcc aacggaaaga tcaggatcat
     5161 agttgagaac tcttgaaaca gtagcaggag aaactcctgc caactgcgca atgtctttaa
     5221 ttgttgccat ttgtctctcc tcctttttta cgtttataaa atcgcagtaa atcgttcaaa
     5281 cgctgcctgt cctttttcat ctcgcttgaa tactccagcg tcttccagta cacgtgcaaa
     5341 aacctgacct acagattttt gtacgacatc ggtgaccgta tctttcgtca aatcaggatg
     5401 ttcttttttt aattgttctg cccacggacg atgatattca gccatctggt tttcttctcc
     5461 taataagaaa tgctctactt ctactagttc tggtttcaaa cgaggtggta aaacagcaag
     5521 ccccattact tcgatcaacc caatgttctc ctttttgata tgttggacat ctttatgtgg
     5581 atgaaaaatc ccatcaggat gttcttctga aacattgttg tctcgaagga caaggtccaa
     5641 ctcaaagtaa ttgtcccttc ttcgtgcaat tggcgtaatc gtatggtgtg gtgtaccatc
     5701 tgctgaaaaa gctctgatag atactgtttc atccgaatat gctttccatt tttccaatac
     5761 tgagttagct gcttcaataa gcaagttttt ctctgttgat tgcaaacgga tcacagacat
     5821 cggccatttc acgactcctg cgatgacttg cggataatct tctagtttga taagtttttc
     5881 tatcggtgct tttgccatag gaaattcatg acgtcctgcc tgataatgat catgagaaag
     5941 aatcgatcct ccaacaattg gtaagtcagc attcgaacca acaaagtaat gaggcaagac
     6001 ttcagtaatc cgaagcagtc tagcaaatgt ttcctttgtg attttcatcg ggcggtgttg
     6061 ttctgataaa atgatcgaat gttcattgta ataagcataa ggggaatatt ggaatcccca
     6121 gctttcaccg tctagattca ttcgaataat ccggtgattg gtgcgggctg gatagttcaa
     6181 gcgacctcta tatccttcgt tttccatgca aagcatgcag gtaggatagt ctacttttac
     6241 cgcctcgcgt tctgctgcga tttgttttgg atctttttct ggcttagata aattgattgt
     6301 gatttccagt tcaccatact ccgactctgc cggaaatttg atgttttttg cgatggctct
     6361 tgttttgatg tagtcatttt tcttacttaa attaaagaaa tactcagttg cttcctctgg
     6421 gcttttcgaa tagtgctgtg caaagaatgc attgacgact gaaggtggcg gtgtgagaaa
     6481 atccattaat tgcgcttcaa aaatctcttg ttctgctggc aattctgaaa gaacaccatt
     6541 ttctcttgcc ttagcgacaa gctgatccaa taagtctaca gattcttcct taactggtct
     6601 tacttcaatg ctttctagag catcttctcc taccatccct aaaagacgat tctgtaaata
     6661 taagcggtcc atttccatcc atccgccagc ttcgatcgca agtgtggcaa aatcacaaat
     6721 cgtttgactg attgacatat ctcattcccc tattcttcat aaccatttgg atggctgaca
     6781 tgccaatccc atgctgtttt gataatcgct ttgatatctg tgtattcagg tttccagccc
     6841 agaatctctc tcgctttttc acttgaagcg actaatcggc taggatctcc agctcttctt
     6901 ggtgctattt ctgctggaat ttcttttcct gtcacttctc tagctgcttc tagcatttcc
     6961 ttgactgagt atcctttgct gctgcctaaa ttgaagaagt tgctttcatt tccttctttc
     7021 aagtattcca aagctaaaat atgtgcagca atcaaatctt ctacttgaac ataatctctg
     7081 atacatgttc catctggtgt atcataatcg tctccataaa cagccaaggc ttttctttga
     7141 ccaagagcta cttgtaaaat gattgggaca agatgcgttt ctggtgtatg atcttctccg
     7201 atagatgcat ctgcttttgc tccggctaca ttgaagtagc gcaaagcgac atagcgcatg
     7261 ccatatgctt ggtcacacca cttcatcatt ttttccatca tcaatttact ttcaccatac
     7321 ggatttttag gattagctgg tgtatcttct gtgattggag actctttcgg ctctccataa
     7381 gttgctgcag tagaagagaa tacgatttta ttcacgttga actcgtgcat gacttcaagt
     7441 aagatttgca taccgtatac gttgttgttg aaatacatca atggtttttc gacagattct
     7501 cctactaaag agcttgcagc aaaatggatt actccttcaa tcggttcctt ttcaaatacg
     7561 gaacgtagaa attctttatc ccggatgtct ccttcataaa aatgagcatc tggatgtact
     7621 gcttgtttgt gacctgtgag caaattatct accacagcta cttgataccc cttttgaact
     7681 agctggtcta cagcgtgcga accgatataa ccggctccgc ctagtactaa aattgtcata
     7741 aaaaacactc ctttgattag taaaattgtg tttgttgatt ctatataatt taaaaaacga
     7801 agtttacttt tatggtgaaa acaaacaaag taaaatttcg taaa
//
LOCUS       DAPCMT010000125         7842 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPCMT010000125 DAPCMT010000000
VERSION     DAPCMT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.128"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(19..993)
                     /locus_tag="RD743_002285"
     CDS             complement(19..993)
                     /locus_tag="RD743_002285"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDU0564082.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1073..1948)
                     /locus_tag="RD743_002286"
     CDS             complement(1073..1948)
                     /locus_tag="RD743_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDU0564083.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2078..3985
                     /locus_tag="RD743_002287"
     CDS             2078..3985
                     /locus_tag="RD743_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDU0564084.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4526..7804)
                     /locus_tag="RD743_002288"
     CDS             complement(4526..7804)
                     /locus_tag="RD743_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDU0564085.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2209 a         1660 c         1461 g         2512 t
ORIGIN      
        1 tttccctctt ttttttgttt attcgaattc tttttcttcc agacggaata acggactcat
       61 cggtgtattt tcatggatgc gtttgaccgc ttcacccatt aattctgaac atgtaacgat
      121 tgttaagttt tctggatgac gattttcttc tgtgaaaaca gaatcagtga tacaaatatc
      181 tttgattggt gcattgtcta aaatactttt cgcatgatcg gacaataatc catgtgacgc
      241 acaggcatat acttcttttg cgccgttttc ttttaataca tttgctgctc gtgcaagcgt
      301 tgctcctgtg ttcaagatat catctaccat gatacatgtc tttccttgaa catcaccgat
      361 cacataacca ccctcagttg aagcatcatc tgcatgatct acgatggcta atgtgctgtt
      421 caaatattct gaaagactac gtgcacgctg aacaccgctg ttttttggcg aaacaactac
      481 gatgtcatct cccgtcagac ctaattgacg gtaataatga gcaaacaacg gcatcgtaaa
      541 taaattatct acaggaatat caaagaatcc ttgtacttga accgtgtgca gatctaaagt
      601 cagtactcga gttgcccctg cttcttcgat caagttagcg atcaatttcg cagtgatcgg
      661 ttcatgaggt ttcgccgtgc ggtcttgcct agcataccca tagtaaggca atacgacatt
      721 gatcgttttc gcacttgctc gcttcattgc atcgatcatg atcagtagtt ccatatagta
      781 gtcattgaca ggtgcattcg ttgattgaac gatatacaca tgatcccctc gaatactttc
      841 ttcgatattg atggaaatct cgccatcact aaattgcttc acgacgcttt tacctaattc
      901 tgttccgaac actttcgcga ttttttcagc taatggacgg ttgccattca aactaaaaat
      961 cttcaacgtg tcatcctgat acttattact catgtagtcc tccccaaaac ttctctattc
     1021 ttcgatagtt taacatattt ttccaagatt ttctgttaat ttcagcaaat tcttaaggaa
     1081 gtgaaactgt tccgttactc caactggcag gaatgacttt atgataatcg ccccagttgt
     1141 cgtgtacttt gtaataacgt tctcctgttt cagtttcaaa ataagcgtac gctgtcaccc
     1201 aatgattgcc atatgtactg ccaagtactt tcaaaatacc aatcatcaca ggtttgcctt
     1261 cacgtatacg ttttgtagct cgctgccatg aacctagaag agttgctcgt gcacgctcat
     1321 gattgccatt cttctttaaa aaactagaaa tccctgtact aacctgaaaa ggaatcgtag
     1381 gaagtcctag tggttggatc tggctacgca atgccttcgt caaaaccaac gaatcagccg
     1441 tatttttctt gcgaatctca tttggaagca tccacccctt acgatagtct tgataatagg
     1501 caaggagaac tgctgccgcg taagtaccac aaagatagcc tgagggggta gcccatttct
     1561 tatacctcaa aaaacgttct tctggcaatc ccacccagca atttgcgagt tcctttcctg
     1621 accttttttc ttcgatcaaa acgggaatat cttcttttct gttgatccat ctagataact
     1681 cgattggctc ttcattgatc agcttccatt ttttacaaga aagaccctct tgataagtat
     1741 attttgctgg agaatattct accggatgtc ctgattgatc gataatcaca taactttgat
     1801 cttcaaaaac cagagctaaa aaaagcaatt tactttttct tgactgcagc tgctgagtgc
     1861 tggctaattt tttaattttt ttatctactt tgaaatataa aggtttatcc actacttgat
     1921 tcagttggga tcgcctcctt tttttcatta taagtgaagg attgaaaaaa acaaatcagt
     1981 ttgattgttc gaattttcaa ttttttatgt tctatttgtt aattccttca ttttgtaaga
     2041 taatgggcac acgaaacaaa aaaggagcga tcgatgaatg aacaaagaac gattgaaaca
     2101 agatttctat gaagcagtca atgaagaatg gcaaaaaaca gcaaaaatcc ctgcagataa
     2161 accagctaca ggcggtttcc aagatttagt tgacgggatc gaccaacttt tgatgggaga
     2221 aacagacgat ctgcttgccc atccagaaaa agtgtcttca gaaaaaatga aacatttcct
     2281 tgcatattat caactggcaa acgattatga ccgaagaaat gagttgcatg ctcaaccgct
     2341 tcaacctata atcgaaaaag ttgaatcatt gcagtctttc gacgaattga ataagcaatt
     2401 tccagaatgg ctgttggata cattaccatt gccatttaat ctggatgtag atgcagatat
     2461 gaagaatgcg caaacgaacg ccttctttgc ttatccgcct tctttattcc ttccagataa
     2521 gacgtattat gcatcagacc atcccaacgg tgcacaacta ataggcgttt tctttgatat
     2581 ggctgttcag ttattagagc ttgccggcaa agaaaaaagt caggcagaag caatcacgga
     2641 acaagcagtg caatttgata aattgattgc tcctcatgta aaaagtgcag aagaaaacgc
     2701 agattacagc aaaatgtata atccgcaatc ttttgataca tttattaaat acacagataa
     2761 actggacttg aaaaaacttg tcacgggttt gattgggaca actccagata aagtgatcgt
     2821 aactgatcca gtgtacttcg atcatttgaa tgacatcctg acagaagctc attttccgtt
     2881 attcaaaagc tggatgatca ttaaaacagt acgttctctt agtggttatc tatcagaaga
     2941 attccgccaa gtcagcggaa tctattctcg cgtattgtct ggaacagacg aagcaatgcc
     3001 tcagaaaaaa gcagcgtatt accttgcctc tggacaattc gatcaagtag ttggcgatta
     3061 ctacggaaaa aaatattttg gcgaagctgc taaaaaagac gtagaacaaa tggtgaaaaa
     3121 aatgatcgcc gtttataaaa aacgtctaga agaaaatgac tggcttgggg atgcaacaaa
     3181 agaaaaagcc gtcataaaat taaataactt aggtatccaa gtaggctacc ccgataagat
     3241 cccagcatta ttcgaccagt tcaaaacagt acctgcagat cagggtggta ctttgctttc
     3301 aaatactctt gttttcagca aactcacatt aaaagatcgt ttcagcaaat ggaacaaacc
     3361 agtggatcgt tcagaatggg aaatgagtgc tgacacggtc aatgcttatt atcatccatt
     3421 tcggaatgtg atcgtctttc cggcagcgat ccttcaagca ccattttaca gtctagaaca
     3481 atcaagctct gctaattatg gtggaatcgg agctgttatc gctcatgaga tttcccatgc
     3541 atttgataat aacggcgctt tatttgacga atatggcaac ttgaacaact ggtggacaga
     3601 agaagatctt acacaatttc aaaacaaagc acaagcaatg atcgaagaat tcgatggtct
     3661 gccttttgct gacggaaaag taaacggaaa attaaccgtt tcggaaaata ttgctgatgc
     3721 aggcggtcta agctgtgcat tggaagcagc aaaaacggaa tcagatcttt ctatcgagga
     3781 attctttgtc aattgggcaa cgatttggcg tacaaaagca aaaaaagaat accaacaact
     3841 tttactgcaa atcgatgtcc atgcccctgc taaactccga gcaaacgtgc aaccaaaaaa
     3901 ccttgacgaa ttctatgaaa catttggtat cacggaacaa gacgctatgt atctagcgcc
     3961 agaaaaaaga gtgcgaatct ggtaagaggt tgtgccagaa gtggtcagct tcaagaaata
     4021 agaaggaatt tgggaaaatc gcttctcaaa tttttgtgac atttcggctt atttttcgaa
     4081 gaagttactt ctggaatacc atgtattaga ggttgtgaaa aagctgctta gtttcaagta
     4141 gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttga cttactaccg
     4201 agaaactggc ttttgaacac cgtttatcga gaggttgtga aatcagcgtc ctgacctgag
     4261 tattagagaa aaaaacggaa aaatagttcc tcatattttg ccgtttgttg agctaatacc
     4321 gagggtcagc tgattgaaca ccgtttagtg gagagattgt gaaaaagctg tcctgcatca
     4381 agtaataaga acagccaatc aaaagcagca aggagaacct catctataaa aagatagaag
     4441 gaagtgagac atctattggt ctcatttcct tctattttta ctttcctcag tcaattgttt
     4501 tacatcataa aaggagaaat tccacttatt tttttcgctg tttcatgaca taaacaacta
     4561 caccgattaa caaaattcct ccagctaatg ctagcaacgg gttattaaat tctcccgttt
     4621 ttgggagtcc ttgagtgcca tttttttcac tttcttttgc tgtcctatcc ggatgagaag
     4681 gttttccatt tggttttcca tttggtgatt catttcctgg attttctctt tctggtttgt
     4741 catttcccgg gggcacatcg gcagtttctc gcacagtgat cgctttttgc tgggtaacag
     4801 attctgtatg agcagaccat ttttccccat caaatatttc cgaacgataa gtgacttcta
     4861 gcgtatattc tccttttgaa ggtgctgaaa aaatagcggt atgattagaa aattgagcac
     4921 ttgactgtgt ttcaacgatt tggatctttt ctgggaaata acgcatatct tcaaagacag
     4981 gcgttccttg atttggtgtg aaagttgaca caaaaactgt aaaaggcttc agttcaatga
     5041 tatttccttc tgcattctcc gccacaccat ttgcttcttg tataataatc ggattatttt
     5101 gttcatttgg ttttgtctga tttggttctt gtacgattgc atactctgga tattccgaag
     5161 acggtgaggt ttctccgtca aaaatagcac ctacagataa tggaagacca tccactcttt
     5221 ccttttctgt ttgacgattc cccggtttaa tctgcatcag tccatgaaca tggatacttc
     5281 cattttcata gcgcgtaaca ggctgctgac cttctttcga acatgtattt agagactcga
     5341 tccaatcttt tgatatggta tgtccaagag tattttctga ttgtttctct cgttcattcc
     5401 aatagtagtt caacgagtgg tagatttgat cttcgttttg attcaatggg cggatctgct
     5461 ctctcatctc aagattctct gttcggaaag ataagatacc agaagcatgg tagtctgtgt
     5521 ttcctgctga agaggtataa agagccacat tatatgaacc gttattaaaa cttgtactgc
     5581 tattgacgat aatatctgtt ccgctattgg aatcgattcc tttcgcaaga ttttcgaaag
     5641 acaaactatt gatcaattca tgagggcccg ttaaagaaga accgcccatt ttgaatccat
     5701 taccgttacc ttctccctca attcctggta cccagccgtt tcgataagca actgagtttt
     5761 tgatgattac ttttccgatc gaacctgttt catttttagc aaaaaggtcc caaccgtcat
     5821 ctgcattatg atacgcgata caaccgtcaa atacatttcc ttctcctatt gtcagtttgg
     5881 ccgcaaatcc atcggcatct tcaaatcctg gatcggcatt ttcaaaagat gtacagttct
     5941 tgactaagtt atgcgcgggc catgcttcga aaggatcatt tgagctaccg ctgatttgga
     6001 ttcctgtatt tccattccga taagcatgaa tctcttcgat caaattatga tttccagaaa
     6061 tctgtaatcc tttttgcata gctgcactat ttgtaatgtc aaaccctcgt aaaccccagt
     6121 agtgactgaa cagtgtcatc ccatttccag ttcctttaaa atcaagtaca ggacgcttgt
     6181 tttctggatt tttctctgca atcaactgaa tcgggttttc tcttgtcccg ttaacgcctt
     6241 ttggaatcgt caatcctctt tcgaaggaat aagttccagg cgccatgacg attgtctgcc
     6301 cagcatatgc gtatctcaat gcttgtgtca gagataatgg attttgtctg cttccgtttc
     6361 ctgcatctga cccttctggt gttacataaa ctgtcgaata tttaggttta cggtagtcca
     6421 ctgtatgaag aatctctttt gtttcataac tagtcatctc tacgtattct tctggcggat
     6481 agtttttatc tggcgtaaaa ctgaaagtaa attcgttcgt tccttccatc agttttgtct
     6541 gataatcaaa ttcccgattc gcagtgatag ccaagttttt tgcgttctta agcacctctc
     6601 ctgttgcttt atttttaatt gtcaataatc cgtcagcatt tgctctaaag gacatagtat
     6661 gatcttggtt tcctgaatga ttactagagg tcacagtgta aatcggatca atcaagcgag
     6721 taggtctttc ttgcccagga ggatctgtcg caggatctga gtaagcaact tcgattcctt
     6781 ctaccttgat cgtcatattc cttgctgcag caaatcctgc ataaataacc gattcatctg
     6841 aagcgtataa tttttcccag tcatacatga tgtcagaagc catttcttct cctgtttcag
     6901 gatcgtaata atatgactct aaaccagtat tcgttttttt catctctacg tccagtttag
     6961 taatcgagtt cgtggccgga aacaaattcc catgaccgtt tccgaatata ttataagaac
     7021 ctgtttcttt ttccaagaac cctgctgaag tctctaaagg tgttgtttcc actgagacac
     7081 tcccaggagc tggcgcagct tgcggatctt cagatgatat tcctgtgatt gcccgtgtcc
     7141 ccaatccaag tcgcatcgta tatttgctgc cgctcgtgtt gaccacttct tgtctatccg
     7201 agtcccagac atattcgatc cgactgccta gtatggcaaa tgaatttgaa taaaatgcct
     7261 gcccaaacaa atgatttttt ggtactgcgt ctcttaccat taaagcaaat ccttcttggg
     7321 cattcgtata ggtccaagat tctatttcga tcgtcgcccg catcctaaag ttgatgtctt
     7381 tggggattgc ttgataataa taggtcattc catctgatcc gcttgactgg aattttccgc
     7441 cgttattcgt ggaagagagc cgcacgccgt cttctaatgg ttcaatggca tacctactag
     7501 cacttgtgct ttctccgaaa acaacccctt tccactcttc atttgcttct tcagattgct
     7561 tttttgtctt ttcattttct gattctttca tcataggatc atttccttct tccgtatcca
     7621 tatcttcact agttcttggt acttcctcca ttgttgacat cccttcaatg ttcacgtgtg
     7681 cattttcttc gttcacagac tgttgttcgt tagcagcaac ttcaacgaca gaaattaaaa
     7741 ccggggtact gagcataagg agattcatca ttagcgcact gtattttata cgttttttca
     7801 acaagtgtat tcctcccgta acaaattaga aagcgtttac tt
//
LOCUS       DAPCMT010000126         7750 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPCMT010000126 DAPCMT010000000
VERSION     DAPCMT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.129"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(46..978)
                     /locus_tag="RD743_002289"
     CDS             complement(46..978)
                     /locus_tag="RD743_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0564086.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTEELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(1469..2104)
                     /locus_tag="RD743_002290"
     CDS             complement(1469..2104)
                     /locus_tag="RD743_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0564087.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGTNIEASDLYLIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNHFDDEDE"
     gene            2613..3755
                     /locus_tag="RD743_002291"
     CDS             2613..3755
                     /locus_tag="RD743_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDU0564088.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            3748..3954
                     /locus_tag="RD743_002292"
     CDS             3748..3954
                     /locus_tag="RD743_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDU0564089.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            4033..5226
                     /locus_tag="RD743_002293"
     CDS             4033..5226
                     /locus_tag="RD743_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0564090.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            5531..7696
                     /gene="acm"
                     /locus_tag="RD743_002294"
     CDS             5531..7696
                     /gene="acm"
                     /locus_tag="RD743_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDU0564091.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT         2637 a         1398 c         1523 g         2192 t
ORIGIN      
        1 agagtacgtg attacaaaat agctgtaata atgtaccaat atttgttatt ctataatctt
       61 ccaattactc ccgttctttt caagtaccaa atcaaattga gatacctgcg ttgctttggt
      121 ctgctggtcg atatactcca ctgtcagcga taccgtgact tgattatcct tacgattgtg
      181 aataggattt accagttctt gaaagatgta ctcttttccg attggtttta atatcccgtc
      241 attcacatag taggaaagtt cactggctgt cgctgtagga tagagcttga agaacgtcgt
      301 taaaaactca ttgatttcat tggttgtaat ggaatcaacc gtcccctcac tttcaatggc
      361 ttttggttta taacttgatt tcttaggtat gttggtaatg gtcggattct taaccagtac
      421 catatttcca gaaccatcta catagacact cactatataa gcagagtgga cggtctttgt
      481 attttctccc tctgtaatga gctggtctac actgtaggtt acattaaact cattgtcgcc
      541 agttggctct accgtccata tctgaaatcc tcttacagaa gacgatacag gaatatcttt
      601 gcgtactgta tcaacattga gagcttgaag ttcctctgtc agatagcctt ttagactttc
      661 cattcgatta tcaatggact tatcggattg ctcccatgaa tagtagactt tcgcaaagtt
      721 ctctacaaaa ttttctacat gatgagtatc aacgtattcc ttttctatga tagttgtttc
      781 gtgaatagta tgagtatcta tagctgtaaa gtgcttgaat atcgcaaagc tgaaactaag
      841 ccctaaaagt acccacaagg caatcacaac ctttttatga ggattgacct tatagacacg
      901 aggtttcttt tcctttggta tctgtttttc tttattctga ttttttctaa atttcatcat
      961 taaatcttcc tttctcattg tttgattcgt tctgctccca gttcttcttg ggataatcct
     1021 ttggtttttc tcaaagaaat caatttttcg ttaaaattca tattcagcac tcctttttgc
     1081 ttttttattg tggtttaatt ataccaaaat cgaggtttaa cattctacca ataagaggtg
     1141 gaaatctgtc aaccgctaat aacattcgct caaaagaata cacaagcgtt aaaatatcgt
     1201 taattgttta cttccataat aaaacttagg gcatagtggg ctggtatcta tcaatccttg
     1261 tgtgttactt tatggcacgt gtagcacaag ctcaaacttg ctagttgtgt tgtctgattt
     1321 atcataaatt aggttttatc cctttcttcc tcttgattac caaccacaga aaagaaaaag
     1381 ccctgtcaag agcttgccac cattggtggt gctttgcact ctttactggg ctttttgttt
     1441 tctgtatctt cttgcaagag gaaaaagatt actcgtcctc atcatcaaaa tggttattcc
     1501 ttaatacttc tcgtaacagt tccatatctt cttttgaatt tgggtttatc attttcacga
     1561 cttgataagg tgtcttatac ttatgtccct ctatgctttc cccaaacata tccatgctgt
     1621 ataaatcatc atagcggtct agtagttttt ggaactttaa atgctggata aattctttga
     1681 ttaggtaaag gtcagacgct tcaatgtttg ttccattgat gtagtcggta atgtattccc
     1741 gtaacttttc taactcatat tccagccatt cttcctcgct gtcaaagaag ttatcatagt
     1801 gtccatgttt cagctctgca tttagtcgtt cttctgctct taaaaactgg cagacttcct
     1861 tttcggattg attgacatat ttccattccc ccgacaacaa ttcattcggc gtaacgtcca
     1921 gcacttccat tatcttttcc agcgtttcat aagcaggata attcaatcct cgttcaatct
     1981 tggaaaggct ctgcatattg ataccgattt tgtccgcaag ttcctgttgt ttcattcctc
     2041 taaattttct tatggtctgt atattatttc ctaaatggct tattttcatt gcataaccct
     2101 ccatatctcg ttatatcttc gcggttataa gtctatcatg cttaatatga tttgtaaagg
     2161 caaaaaaaga tattgacaaa taagcatata agaattacta tgttcttgca attaaataag
     2221 cacaatagaa tgaaatacaa aaatattcaa aaagaaacac ctatctgatt aaacccgtag
     2281 atatgaatat tggttaaaat ggaacttgtt agaaagacaa aaacaggact ggctgacaaa
     2341 gcattgtagg actgctggcg tgcgtcagca acaacgccat gacggcttgc cgtcaaagga
     2401 cggaaatgct ttgtcggctg gcgagcctgc gagccttgac tgtgcccccg ttatctaaca
     2461 ggggggcact atttcacaaa agacaagtca aaaaccatac acccccaaac ctgtgagtag
     2521 taatgagagg ttttaagaag atagtacata tcacactttt tgaaaggtgg tttacaggta
     2581 tttcaaggct attgtgggat tggaggaata caatgaacaa tttagaattt gcacttgaaa
     2641 tgaagaacaa acgtcttgcc agcggtcttt cacaaggaga acttgcaagt cttacccatg
     2701 tatcaagata tagtatcaac cgctttgaga acggaaaagc taacgccagc aaggaaacac
     2761 agaacattat cctgcgttgt ctgaattact atatctgcga taagcctttt tatctgctcg
     2821 ttgattatct gtctgtccgt tttccaacga ctgacgcatt ggaagttatc cgaaaggtgc
     2881 ttggcatgaa agcagattat tttatccatt atgactatgg gtattatggc tataaggagc
     2941 attatgctta cggggaaatc aaggtcatgg cttctgatga tgaacacatg ggcgtatttt
     3001 tagaattaaa aggggcaggc tcacgcaaca tggaatatgt cttacaggca cagaacaggg
     3061 actggtattc attcttaaac cgctgtcttg actgtggcgg tgttatccga cgttttgact
     3121 tggcaatcaa tgatatgtgc ggtctgctgg atattcctgt gctatctgaa aaatacagaa
     3181 atggtggtgc ggaatgtcgc tgtaaaaact atgaaaatgt acaaggtgga aaattaagcg
     3241 gaaaaaaccg taatttagca agtacccttt atatcggctc aaaggcaagc acaaaatatt
     3301 tctgcctgta tgaaaaacaa aaggaacagg caacgaaaaa gaaacatacc gatattatca
     3361 accgttttga aattcgtctg cgtgacaaaa aggcggtaca ggcagttgaa gaactgttat
     3421 tgacgtataa ccctcatggg ctggtgtttt atctcattac cgattttgtg gaatttcctg
     3481 actatccatt gtgggagata ttcatttccc atgacagttt accttttgaa atgaaccctg
     3541 tacctgtcaa tatggaacgc actctgcaat ggctggaaag acaggtcatg ccgtcaatcg
     3601 tgatgataga ggaaatcgac aggctgacag gttcaaacta catgaaaatg attgatgaat
     3661 gtacccacct ttctgaaaaa caggaaatgc ttgtggaaca gatgtgtacg gacatatcag
     3721 acgtaatcga aagtgagggg gtgttttatg agtaatacac aggatattcc cgtatgggaa
     3781 aaatacaccc ttaccattga agaagcgtca aagtatttcc gcattggaga aaacaagtta
     3841 agacgattgg cagaggaaaa caaggacgct ggctggctca ttatgaatgg caaccgcata
     3901 cagattaaac gccgacagtt tgaaaaggtt attgataaat tggacgcaat ctaatgcaaa
     3961 tgagccttgt atgtgttatg atgaacacaa gtcatatcaa ggctctttcc aacaaggaaa
     4021 ggagcagaca ccatgaaaga aaaaagacgg gataacaaag gtcgtatcct gcatactgga
     4081 gagagccaac gaacagacgg aaaatactta tataaatatg tagacgcatt gggaaacaca
     4141 aaatatgtgt atgcttggag attgacaccc acagacccga caccaaaggg aaaacgggaa
     4201 aaaccctcac ttcgagaact ggaacagcag ataagacggg atattgagga cggtatcgac
     4261 agcacaggca agaaaatgac gctttgccaa ctctatgcca aacagaacgc acagaggaca
     4321 aatgtgaaga aaagcacaca gaaacaacgg gaacaactca tgcggttatt aaaagaggac
     4381 aagttaggtg ctaggagcat tgatacgata aaaccctctg acgctaaaga atgggcgtta
     4441 cgcatgaaag acaaaggctt ttcctacaat accattaaca accataaacg ctcgttaaaa
     4501 gcgtcattct acatagccat acaagatgat tatgtgagga aaaacccttt tgatttcaag
     4561 ctaagtgaag tcatagaaaa tgataccaaa gagaaagtcg cattgacaga ggaacaggag
     4621 caagccttat tgtcatttat taagacggac aacgtgtatc acaagcatta tgatgatgtg
     4681 ctgatactgt taaagacagg acttcgtatc tcggaactgt gcggactgac agtagccgac
     4741 attgatttca agaatgaagt tgtgattatc gaccaccagt tactaaagag taaggaacag
     4801 ggctattata ttgaaacgcc taagacgaaa agcgggataa ggcaagtgcc attaagcaaa
     4861 gaaacgatac aggcatttca acgggttatc aacagccgac caaagataaa gtcgatagag
     4921 gtagacggac acagcggttt tgtgtttatc aatccgaaag gtaatccaaa agttgccatt
     4981 gattataacg ccttatttat ccgtatggta aagaaataca acaagcacca caaggacaat
     5041 cccttgccac atattacacc gcatacgcta cgccatacat tctgcacaag gctggcaagc
     5101 aagaacatga acccgaaaga tttacagtat atcatggggc attcaaacat cagtatcaca
     5161 atgaactggt acgctcatgc gtccatagat accgcaaaat cagaggttca gcgtctaatc
     5221 gcatagaagt atttaccacg attttaacca cgattgacag ggaaaatata agaagataga
     5281 cctagatatg cgaggtttac cacaaaagca aaatgcccgt agagccgata aaataaggct
     5341 ttgcggacat ttgagaagat ataaaaagat agtcaaaaag acatatataa ttttttataa
     5401 atatttgtaa aataactatc aattttttag ttaaaagatt ggaaatgagt gggggaggaa
     5461 ttgaagaaag gatttttgag agatgatata gtaggtttta tttttagtta aaatagggga
     5521 gatagaaaaa atgaaaaagt gtagaaatat tattttatca atgttattta ttattactaa
     5581 tataacatca ctgattcctg ttcatgtcta tgcggatgca ggcagagata tcagcagtaa
     5641 tgtcacttcg ttgaccgtgg acccaacgaa tattacagat ggaggaaaca taaaagtaaa
     5701 attctcgttt gacgaaaaaa aacaaaatat ccaaccaggt gattatcttt ggatcaattg
     5761 gccaagtgaa ggaaatattc gcggtgaagg attccaaaaa gaaatcccat taatgattga
     5821 aaataaaaat gttggaactt taacggtcag aaaagattct gcgcaggttg tgtttaatga
     5881 aaatattaaa aacctagact cagtagaagg ttgggggcaa tttgagatac aggccagaaa
     5941 cgtaaccgat accggtgaag aaaatacggg accattcacc gtgacgagcg gtgataaaac
     6001 agctactgta aatgtgacaa agccagcttc tggttcttct tctagcgttt tttattataa
     6061 aacaggagac atgctacctg aagatactaa gcacattcga tggtttttga atattaacaa
     6121 taacggcact tatgttgaac aaccagttaa aatcagtgat gaaatccaaa gtggacaaag
     6181 attagatcca agtacgtttg aaattaatca aatacactta ggtgaacaga aggtttatag
     6241 aggagaagag ggaatccaac aatttttaca agatttcccc agcgctacat ttaatttttc
     6301 agtaactgat aattacattg aaataactat accaaaaaac tttgtcaatc ttaggaaaat
     6361 aatggtgagt tataagacga tcatagaaaa cccagaacaa ataaattttg agaatcattc
     6421 ggaagcttgg tttaaagaat ttaataaacc tgctgttgat ggagaaagtt tcaatcatac
     6481 agttaaaaat atttcagctt ctggtggagt caacggtaca gttagaggcg aattgaagat
     6541 tttcaaatat atcaatgaca cagagattgg tatcccaaat gtgactttcg aattaagacg
     6601 agcagatgaa cagcctattc aaggacagtc tagtatcctg ttgacttcga atgaacaggg
     6661 agaagctagt atcaaaggcc tgcaagtagg agactatgtt gtaaaagaaa aagaagcacc
     6721 caattggatt gattttgatc cactaagttc aaatgaactc aaattctcca taaacgaaaa
     6781 tgatacagag ggagtcagtt tacctattta taacaagaaa aaggttacga acatcacagc
     6841 aactaaaatt tggaacggtg gaaccactcc aagaccgagt atttatttta aattattcag
     6901 agcaagtaca aacaattggg aaccagttcc tgatgcagaa acaaaacgat tagacaatgg
     6961 aataacttca gtgacttggg aagatatcca acagtatgat gatagtggaa acgaatatac
     7021 gtttaaagtg caagaagtag atcaaaacgg aaatgattat gtcccttcag gttatcaaaa
     7081 aattgaaaac gggctagtcg ttacaaatga gaataaagaa gtcatttcag tctcaggaca
     7141 aaaaacatgg gaagacaatg agaaccaaga cggcaaacga ccgacgacga tcacagtaaa
     7201 tctattagcc gacggtcaac cgatccaaca caaagaagta agtgaaaaag atgattggcg
     7261 ctatcgtttc acaaatctgc caaaatacag agacgggcaa gaaatcatct atactgtaac
     7321 ggaagacaac gtgcccgaat acagtacgac gattgacggg tataacatca aaaacagtta
     7381 tacaccggaa aaaacaaata tatctatacc aggtagccat ataacaccgt ggaaaaattt
     7441 tccagagaag acagagagag agaaaattag aaatctattc tctcaaaaat tgcaaatatt
     7501 tgctcataca ggagaatcca gcgaacaaag aagcaattat attagtaaga gagctgtacc
     7561 taaacagata acaaataaat caaaaagcat tcttcctaaa acaagtagta aaaaatctag
     7621 ttttgctgta atcattggtt tattgatagt aactatctgt gctgggatat tcttacaaaa
     7681 atataaaaaa gtttagttag aatataatat tttttgttaa atatgactga aatcaaaaga
     7741 ataaaattcc
//
LOCUS       DAPCMT010000127         7674 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPCMT010000127 DAPCMT010000000
VERSION     DAPCMT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.130"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            46..2373
                     /locus_tag="RD743_002295"
     CDS             46..2373
                     /locus_tag="RD743_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDU0564092.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2499..2828
                     /locus_tag="RD743_002296"
     CDS             2499..2828
                     /locus_tag="RD743_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDU0564093.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2868..3872
                     /locus_tag="RD743_002297"
     CDS             2868..3872
                     /locus_tag="RD743_002297"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDU0564094.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3869..4315
                     /locus_tag="RD743_002298"
     CDS             3869..4315
                     /locus_tag="RD743_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDU0564095.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4465..5280
                     /locus_tag="RD743_002299"
     CDS             4465..5280
                     /locus_tag="RD743_002299"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDU0564096.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5486..6658
                     /locus_tag="RD743_002300"
     CDS             5486..6658
                     /locus_tag="RD743_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDU0564097.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6820..7314)
                     /locus_tag="RD743_002301"
     CDS             complement(6820..7314)
                     /locus_tag="RD743_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564098.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
BASE COUNT         2583 a         1451 c         1531 g         2109 t
ORIGIN      
        1 ataacgtgta aaggattatt tgatgatttt ttattgagag gagggatgaa aatgattaag
       61 aaaactcaaa ataaaatcca acgtatatgt caaaaacatt ttgtttctag actcaaagct
      121 gtatctaaaa gaaaatactt acatcccatt atgatttcga tcatcttgat ttttactttc
      181 ttagggagct tatcaagtat ttatgtaaac gtttatacag aaacgtcaaa ttttgctaac
      241 caaacgacag aggagacaaa tagtccaccc tattctgaaa tacagtccat agaaagcacc
      301 aatcagtcta gcaacaacga gacaacctca accacatcct cgactcaact gattgatccg
      361 acagaaaaag aaatcacagc agcaaaagca gcaggggaga aagcttattc agaaacaggt
      421 agaagccaga catttagtac tgttgcggcc actgcgaatt tcaacggaca taccatacca
      481 gatggatata cagtgatata tgccaaagat ggagtcattg ttgcggttaa cggatcgatg
      541 agtgcggata aaactgcgtt acaaggcgta accaaagcaa tcgcttttat tgataaccgt
      601 ccagatatcc ataatctgtt tattggctcc acagtaaatg gtactactat cacattccca
      661 aaaagtatct atatgggggt ggctaccgct ggaaatacga tcgattcagt tacacccaat
      721 gcggataaaa gttattcttt tacaccctca acatggggag ggcttcctgc cggaggaggt
      781 atgagtactt tttcaatcgg tatacagtta gcaaatgata ttccattcac ggggtactcg
      841 atcaaaaatg ggcagatcgt tgatggtaag ggaaatgttt caaattttcc aacctatcaa
      901 gcagcaacgg ttcaggtcaa tgcgaatcgg acaggaactt tttcttatac agtcaatggt
      961 ggagcagcaa aaatcggaac gtacgggact acattatctg gaatccatgt gggtgacaaa
     1021 gtggttatca ctccaacagc agtaacgggc tattcttttg cacaaacaaa cggttctttc
     1081 acagccctag aaagtaacgt cgatacggtg acctatacag gcaaacaaac accaatcact
     1141 attaagtatc aagactattt tggacagatg attgctcctt ctaaaataat gaatcttact
     1201 tacggaagtg ctgcacaaga cctgacgact aatacgccta ctatcaacgg atataccttt
     1261 acagctgttt cagcgataga aacaaagaac caatcagcaa tgagtgtttt agctagttta
     1321 gatacaaatg gaaatgtaat cgtaaaggac gcaaatggaa aacagatttc tgaagtcatc
     1381 ctatattata agacaaatgc aacagtatca atcaatgcaa atggcagtaa gtactatgat
     1441 ggtctttctg ttgtaccgat cgttaaatat gcctttaatg ataaaacaga atcaacatcc
     1501 cttttaaaca atgatctcag tattgatcaa atcacatgga atccaactga tttcaaagcg
     1561 atgaatgaaa agggagttga agtaactgcg cctactgaaa ttggaaccta tacgtcttgg
     1621 caattgacaa aaagcggact agctaaacta gctgccagaa caaactattt atttactatc
     1681 gtgcagacat ctgatgtcta tacgattaag caaatcagcg gtgcggtaga gctcagtggc
     1741 agtaagacat atgatggaaa agcaggtgtc ccgtcaatcc atgtcaagct agcagagggt
     1801 gtcactagtt cgcttactcc agtacaagtg gcacttagca gtatggatta cacagcagat
     1861 gcacaatcag caaaaaatat ggttaatgct ggcagctata caattaaact aacgaatagc
     1921 ggaatagaca aagtcaaagc agcaaatccc aagttgtcat ttactgatat agccaacaca
     1981 tcaggaactt ataccatcta taaagctaac gcagtgatta ctgtcgatga cgctgccttt
     2041 aattatgatg cccaaagtca ctcgatttcg gttggaaatc tccacgttac cggtgttgtc
     2101 ttaggcgaat cattggatta taccctaaca gttaacagcc ggacagatat tggatcacaa
     2161 atagtaacga ttagtttagg cgaaggaagt gtaaacaata attatacgat tatcgcaaca
     2221 gataccgcta agttgacgat caatccgact ctcttgcttc cttcgacagg tgggataggt
     2281 ctactgccct ttgtcttttt aggactgatc tttatttttt ctggcttctt ttattttatc
     2341 catcgtaaga aaaaagcagg tgaacagcga tgaaaaaaac aaaaaatgta cgtgtggata
     2401 tatttgctgt tctctttttt atcctactgg tcttttttat aggttctccg actgtgtcag
     2461 ctagagaatc acctattaca tttactttgc ataatcgaat gttcgaaagc gataagatgc
     2521 caagtatcag tcaaaacgac ggaataaata tcccggataa ctttcaagga gtaaaagggt
     2581 tgaacggcgt tactttcgct atatatgatg tctcggatga attttacaag ctgcggtcag
     2641 aaggaagtag tgtggaagac gcacaaagaa aactagcaca aaaaagtgat agtgaaaaaa
     2701 tactggcaga aaatgtgaca aaaacagtag acgaggaaga gggcattgct tcttttagtg
     2761 catcggacaa agatgagcaa ggtcgtgacg ctgtttaccg atttacggaa ataaagactt
     2821 cagattaagt aaaagaaaaa tcagcaccat ttgtcgttgt gcttcctgtg acagattcat
     2881 ctaatcataa attgacgacc atccacttgt atccaaagag cgaacaaaaa atacctgctg
     2941 cactcacatt aacaaaaaca gtagagaata agcagacaga ttttgcagat ggggataaag
     3001 tcccttatct catcaccgta acaattcctg agaacatcaa cgagatagga acctatacga
     3061 tcaaagatac agctgatcct cagttatggc tagaaccaga aacgatcgat tttttgattg
     3121 gtggagacaa gattcataca tttcacacac aaaaaataaa acatggtttt gtcttatcag
     3181 attctggaaa agatcttagt tcgtttgctg gaaaaaaatt aacgatccgt taccaaatga
     3241 cattgaaaga aaacagtgaa aaaacgacat ttgataacga tgtacgtttg acgactgaca
     3301 atcaaacagt agaaacacat cttgccatcc aaacaggagg caaacaattt gtcaaagtgg
     3361 atcggcaaac gaaacaaaaa ctagcagatg ctcaatttct agtcaaaaaa aatgaccaat
     3421 acttagcgca agaaaacggg ataaatcatt gggtagccaa agatgattca agagcaacga
     3481 tttttacttc cagtaaagat ggaacatttt ctgtccatgg attgtctttt ggaagttacg
     3541 aactagtgga gattaaaccg cctaaaggat atatattgag tcaatcaacc attctctttg
     3601 aagttgaaaa aggcagttat gatccgcttc atcctatacc attggaaatc atcaacgaac
     3661 caaaagcaaa tccgacgaat aatggaaaaa cgccagttca gatgcagaca aatggtccat
     3721 caaatgaaaa aaatggagga agctttccaa aaacaagcga agagatgctt ggaggtttct
     3781 caattttagg gttattgcta gtcctttcaa cagggacagc ttggttttat aaaaaacaac
     3841 aaaaaggaaa caataggagg gaaataaaat gaaaaacatc aaaacaaaaa tatggacgtt
     3901 tttaggaact gctatcatgc tgcttccttt cgttctcggc ctaggaactg cagaggtttc
     3961 tgcagccgta tcacctacac cagaaaatgt tacggttaat ttgcataaat tgaaattcac
     4021 ttcagcacca gaaaatcaaa taaacaacgg aacggaattg acatttccga atagcgaacc
     4081 gttgaacggc gttgaattca acgtatatga catcactgct acgtactatc caagtaaaga
     4141 cacagcagtt ccagcagacg ctacaccctt cgcaagtgta acgacttctg gtgaaggatt
     4201 agctaacctg actcttccag gtaaatctga tgggaaagat gctgtatacg tctttgtaga
     4261 aacaccaaag cccggtgtcg aaacgtcgcc aaatatcgtg ttaagcttac cctagaccct
     4321 gacaaacctt tagacacgat tcacctatat gctaaaaatg tcatcaaaga aagcaatatc
     4381 aaaatccaga aagctgggaa cttttcaggg gtagataagt tagcaggggc gcaattccag
     4441 atcaaaaatt cagagaatca attcgtgacc ggttttgatg aaaacactgg tgtcgctctc
     4501 tatgatcctg ctgagtcagc gttgacattc acaactgata ctgaaggatc catcagtgtc
     4561 aatggattaa tagacggcaa ttatacttta gttgaaacaa aagcaccaga tggttatcaa
     4621 ttaaccaatc cacaaaaaga aactactttt atagtaaaaa acggaaaatt gcaacaaaca
     4681 accggtatca tccatggcgc agatgaagcc aatcttggga atccaaacgg tgtagtcaat
     4741 cgggaaaata caactacgga taacacgatt actgtagaaa atgtccaaaa ctacggtgat
     4801 ttccacttca tcaaacaaga tgtgaacacg caagaaaaat tagccgatgc agaattttct
     4861 attctaagag caaaagatcc aactgttaaa ttaatgaaaa aaatagcaaa ccttgcctca
     4921 ggagaatacc catatgcttg gtcagatgaa gtcacaggtt ctgactggga agaagtgaaa
     4981 ttgatttctg attcagaagg gaaattttca atcgaagggc tgaaacaagg ggcatactgc
     5041 cttcaagaaa caaaagcacc agaaggctac gcattaccta agaatgaagc tgcgtacact
     5101 gaatttacag tagatgatga tccaacaact tctgatgaaa caacgatcga taaccaacct
     5161 aaaggaatct tgccttcaac tggtggtatg gggatcatcg tattcatctt ggtaggtact
     5221 gctttagttg gaggagcagt gatctatttc aaaaaacgcc atcaagaaac agaagcttaa
     5281 actattggaa acaaaaatca tacgataaat aaacgatagt gattcaaatg gggagaaagg
     5341 ttgtgacaag tggtcggtct cttggtattc gctcaacaaa cggcaaaata ggaggaacta
     5401 ttttgccgtt tgcttcgcta atactcaggt caaggcgctg atttcacaac ctcttcatat
     5461 acatattaga aaataggtgg acatgatgaa taaaagcaga ataagaatac tggacatctt
     5521 catggcaatc attttagtcg ttgggatcgg gatcttttct tatccttttg tagaagattc
     5581 actgaatgat tttcttgccc agcaaatgat catccattat caaaagcaag catcgaataa
     5641 gaattcagag gaaataaaaa aacagcagga aaaaatgaca aagaaaaacc aacagttggc
     5701 agagcaaaac gtatcaccag ggataggcag tttcaaccaa gcagttgatg caaaagcatt
     5761 aaaagattta cctagtaatg ccttttttat ggaacatatg cttggagtca tcgaaatccc
     5821 aaaaatcaat gtcagcttac ctatattcga tcagacaacg gaaatatttt tgcaaaaagg
     5881 aacgtcatta ttggaaggaa gttcttatcc gactggcggg aaaaacaccc atgccgttct
     5941 ttctgggcat cgtggactcc cagaagcaaa attatttaca gatttgccta agttgaaaaa
     6001 aggagaccag tttttcatcc agatcaatgg aaagacattg gcgtatcaag tagaaaaaat
     6061 ccaagtggtc ttacctgacg aagtagattc tttgggtatc caaaaaggca gagatctcgt
     6121 gactttgctt acctgcactc cttatatggt caatacacat cgactgcttg tgacggggca
     6181 tcgtattcct tatcaagcaa aagaggcaaa aaaagcgatc caaggaattg atcaatggaa
     6241 aaaatggaaa ttttttattt ggtttatcgg tatactactt ggatcaatcg gcttagtttg
     6301 gctactcatc gcttatttag attctttggc catcgctaag cgaaactatc ctttatcttt
     6361 ttatgtaaag aacacaaacg gacgaccaat cgaaggaatg gtcttttctg taaaaacgct
     6421 caacggcaaa cactatatta ctagagaaaa agttccattt gtgaaagctt ctgatgaata
     6481 cggactagtg agattttctg atttaaaagg caggaactat cgcttgcaac atgaggagct
     6541 cctgcttaaa atccatgtaa aacataaaca ttcgaaacag ttttcaatga aattgaaaaa
     6601 aggaagatat aaactgcgaa aagaaaaaga ggtgtactat ttgattgaga aagaatgaat
     6661 gaagatgtcc aaaaaagcag agggaacaga ctaaatgaaa aaagagagaa gctgacgaaa
     6721 tggacgtgta aactgctatt tttggtttac tatctggaac gccagcttct atttttttat
     6781 gtttcagaag aattttatta ttatagtata acttttgaat tagtttccat aacatttgga
     6841 gcaaggggtt aaaccacgag cttgagcttc gcttaaagtg gcaggggtat acgtaccatt
     6901 tccacattta tgtgtatggt attttgaacc ggtcggtgtg acatagacag tttgttcatt
     6961 attttgctca ggagctgcgg cttgtgcctg ctgagaagcc tgttcagctg ctgcttgctg
     7021 tcgcgcctgt tctgcttgtt gctgtgcgat tgcttcttgc ctcgcctgtt ctgcttgttg
     7081 ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt cgtgcaattt cttcttgctt
     7141 tttccgttct tcttctgctt cttttgcttt gattgtggaa tcaattgcag caagacggct
     7201 tgtcaactct tgtttgtttt caggtagcgc ttggatagct gctgaagcag ttgcatagtt
     7261 ttcttttgta ggatttgctt cagcttgtga caaagcagat tccgcttctg tcactttttt
     7321 ttctagtgca gctttttccg ctttttgttt ctcttcttct tttttctttt caatttgttc
     7381 tttcgatgaa tctgccgtac tttcggagat cgtggtactg ctgctggaag cctgagctga
     7441 acttgtgctt tgagtagtag acgtagtagc accacaccca ctcaacaaac ttcctgcgac
     7501 caaaaacaac gcaactaatt ttttcactaa atttcctcct taagtaaaaa acaaccgttt
     7561 tcaataaatg tccttttcaa ttgtttactt acttgttata ataattgtgt tatgaatagt
     7621 gattgaggaa acttaatcta atcccgtgtt gatacacggg attttttgct atag
//
LOCUS       DAPCMT010000128         7632 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPCMT010000128 DAPCMT010000000
VERSION     DAPCMT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.131"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(62..790)
                     /locus_tag="RD743_002302"
     CDS             complement(62..790)
                     /locus_tag="RD743_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0564099.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(803..2083)
                     /locus_tag="RD743_002303"
     CDS             complement(803..2083)
                     /locus_tag="RD743_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDU0564100.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2224..2826)
                     /locus_tag="RD743_002304"
     CDS             complement(2224..2826)
                     /locus_tag="RD743_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDU0564101.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3071..3277
                     /locus_tag="RD743_002305"
     CDS             3071..3277
                     /locus_tag="RD743_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564102.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3589..4308
                     /locus_tag="RD743_002306"
     CDS             3589..4308
                     /locus_tag="RD743_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDU0564103.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4389..5072)
                     /locus_tag="RD743_002307"
     CDS             complement(4389..5072)
                     /locus_tag="RD743_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDU0564104.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5290..7476
                     /locus_tag="RD743_002308"
     CDS             5290..7476
                     /locus_tag="RD743_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDU0564105.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2735 a         1284 c         1438 g         2175 t
ORIGIN      
        1 aaaagaaccg aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt
       61 tttatgaaat caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca
      121 acgtgacttc tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg
      181 atttatgaca acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga
      241 attgtaattg ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag
      301 aaaaatattg gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga
      361 agatatcttt ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct
      421 cgacattttc ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt
      481 tgctttttaa gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta
      541 aaggatctaa ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta
      601 gttcgatatc taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa
      661 gttcttccgg atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc
      721 tatttttgat caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa
      781 tgggaatcat atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct
      841 ggttttcaat tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga
      901 taaacaagcc aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca
      961 actggtttaa cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat
     1021 tcttcttgag tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg
     1081 cattgtccaa gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt
     1141 cgattggctc aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt
     1201 ttaatgacag taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg
     1261 ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta
     1321 atccttctaa gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat
     1381 catgtttgaa ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt
     1441 gcatcctgac ttgttgctct aataactgat cagctaattt cttattagag attttccaat
     1501 aataaaccaa gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag
     1561 ctaagacatc aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa
     1621 ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct
     1681 ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt
     1741 gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgt
     1801 cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa
     1861 ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa
     1921 ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca
     1981 gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa
     2041 ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt
     2101 gatttttacc aaattatagc acaattagcg tattattttt ttaatttttc agggattttg
     2161 aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat
     2221 tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat
     2281 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
     2341 agctgagaaa aataaagtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
     2401 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
     2461 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
     2521 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
     2581 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
     2641 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
     2701 tagttttcca agattattca ataaaacttc taaggcaaat tttacttgga catacaccat
     2761 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
     2821 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt
     2881 tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt
     2941 tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca
     3001 agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg
     3061 agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc
     3121 tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat
     3181 gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca
     3241 gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca
     3301 taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag
     3361 cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta
     3421 tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt
     3481 acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt
     3541 tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt
     3601 gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc
     3661 caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta
     3721 ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc
     3781 tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac
     3841 gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta
     3901 aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa
     3961 aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc
     4021 aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat
     4081 aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata
     4141 gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta
     4201 caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc
     4261 tacactacaa ataacccgaa agcaaaggga atctttacaa ataaataata taaaaactaa
     4321 gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt
     4381 cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga
     4441 gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga
     4501 tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag
     4561 tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt
     4621 gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta
     4681 tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga
     4741 tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca
     4801 aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga
     4861 agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct
     4921 ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca
     4981 ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct
     5041 attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt
     5101 tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa
     5161 tcatatcgct tcccattttc atttttagaa aggtctgtta gtataaagaa aaaatagaca
     5221 acgaaacttt aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa
     5281 atcaatcagt tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag
     5341 aacaagtata aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat
     5401 gaagcggctt atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt
     5461 gaaggaaaca gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa
     5521 gaacaaaaag cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct
     5581 gggagcggca aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa
     5641 gtagacgaag aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa
     5701 aaacgagtag ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt
     5761 atctttttta ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga
     5821 atacaaggag acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg
     5881 atacgtaaat ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg
     5941 ttagactatt ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa
     6001 aaatatgatt ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg
     6061 ctcacggaac tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc
     6121 caaaatctga aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat
     6181 gattatctat tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa
     6241 cggatgacaa aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc
     6301 tatggattca gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc
     6361 aaaactcttt tcttattgac taattatcgt tctcgggcag ctattgtcca aggagcaaat
     6421 agattgatca gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa
     6481 gaagcaggtg ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta
     6541 atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta
     6601 taccgatcag cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct
     6661 tttgtactgg aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat
     6721 ttctatcaaa aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc
     6781 taccagcaat tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat
     6841 caatactttt ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc
     6901 cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca
     6961 gaccagcatc aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct
     7021 aaggaaatcg accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact
     7081 ttcagaagac tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc
     7141 cgcttgattg cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa
     7201 agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct
     7261 tataacggag cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagtg
     7321 acgacgatag aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt
     7381 ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc
     7441 aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt
     7501 ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat
     7561 ttcagcttat tttcgaagaa gctatttctg gaacacagtt tattcggaaa agggaggtgt
     7621 gacaagaatt tt
//
LOCUS       DAPCMT010000129         7551 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPCMT010000129 DAPCMT010000000
VERSION     DAPCMT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.132"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..704)
                     /locus_tag="RD743_002309"
     CDS             complement(<1..704)
                     /locus_tag="RD743_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDU0564106.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDM"
     gene            complement(704..1840)
                     /locus_tag="RD743_002310"
     CDS             complement(704..1840)
                     /locus_tag="RD743_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDU0564107.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1872..3323)
                     /locus_tag="RD743_002311"
     CDS             complement(1872..3323)
                     /locus_tag="RD743_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDU0564108.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3367..4131)
                     /locus_tag="RD743_002312"
     CDS             complement(3367..4131)
                     /locus_tag="RD743_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDU0564109.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4159..4857)
                     /locus_tag="RD743_002313"
     CDS             complement(4159..4857)
                     /locus_tag="RD743_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDU0564110.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4869..5648)
                     /locus_tag="RD743_002314"
     CDS             complement(4869..5648)
                     /locus_tag="RD743_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDU0564111.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5664..6608)
                     /locus_tag="RD743_002315"
     CDS             complement(5664..6608)
                     /locus_tag="RD743_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDU0564112.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6654..7433)
                     /locus_tag="RD743_002316"
     CDS             complement(6654..7433)
                     /locus_tag="RD743_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564113.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2110 a         1606 c         1163 g         2672 t
ORIGIN      
        1 tccatatcac cagttccaat aattaataat acggaattat tcttaatagc gtgaaattct
       61 ttaaaaattt caatcaaaaa acgatgattt ttagaatatg aaattcttcc aatatgtccc
      121 attacaaacg tctgacttga aattcctaat ttcattcgta catcatttct aatactttca
      181 tcataataaa attttttagc atccactgca tttggaatca aagtgaattt atcatttcga
      241 aacaaaaaat cacctgctac ttttccacat gaaaattcat gtgtcaaaac agacgttaac
      301 ttacatcgat aatattgctt taaaagatat ttaaaatctc tatctatcga tgtattatgg
      361 ctatgtgcta tccgtatcgg aatcccagct ttttttgcag cttttaaagg taaataactc
      421 atcgcatcaa tatgagaatg aacaatctca tattctggat gatccttaaa aaattgttcc
      481 atccatttaa aatatttagg ataattttgt ggataaagtc tcggtgcata atagaccttt
      541 cctccaagac ttattatttc ctcatcataa tcacttttat atggacgatg tgtcaaaaaa
      601 tcaaactgaa ttttagttcg atctatattc ctataatagt tcattagcat cgtctccaac
      661 cctgcacgat gcatatcatt cacacactgt aatattcgaa tcattatata tttcccctat
      721 acaaatttaa tgtttcttgt acgattttat cccaattata tttactacaa ataaactcag
      781 tagcttgatt cttcaaaact ttaaccattt ccgattgatt gcatgcctct tctaatctta
      841 tctttaaatc acttacatcc gatttcttaa aaatcatagc cttgtcttct actacttccg
      901 tacactctga gatatttgaa actatacaac agttcccata actcattgct tctaacaaac
      961 ttaatggcat tccttctaaa tcacttggaa gtgtatagat atacgcatta ctatataatt
     1021 cttctaattc ctgtccttgt acaaatccag taaatataat tcgctcatca ccttttgcta
     1081 attcctttag ttcatttgca aattcatctg tatcagagct tcctccggct attataagtt
     1141 ttttatctgt ctgaacatct ttaaatgcct caattagata ccgaattcct ttttcgggaa
     1201 ccaatcgccc caaaaacaaa atataagaat cttttgttaa tccatacttt tctgtaatca
     1261 atcctgctga ttttatttca atttttttaa cgccatttgg tataaacctt gtttttcttc
     1321 catatatatc ttcaaaataa ttttggacgc tttcactaag tacaattatt tcatctgcaa
     1381 attttacagc acacttttcc cctaacatga tgtatgtact agcaagcttg ttccattttt
     1441 ctctttgatg atctagacca tgaattgttg caacacatct tttcccaaaa agttttggca
     1501 accacaacat agcacatggt ccttctgcat gaaaatgaac aacatcatat tttccgaatg
     1561 ctgctctaat tccaccaaat actgaagaac tcattgctgc aattcctttt atatttaatg
     1621 taaaaatagt ctttaatttt acacctttat attctttatt tttatgctgg tcaaattcct
     1681 ttccgctaac atgatgacca cttcgattat agcaagtcac ttcatgccca agtgcaacca
     1741 ttcgcgttgc caattcttct accactatct caattccacc ttctcttgat ggaattcttt
     1801 tatgacctag catacagatt tttaattttt ttcttgccat cacgttttac tccttctata
     1861 gtatctttat cttacatgga accgtttcta tgaagcacag ttttgactgt cctaaacaaa
     1921 attctaaaat ctaatcccat actccaattc ctgatatatt ctgcatcaag ttttacaacc
     1981 tcttcgaagt ctgtgatatc acttctacca ctcacctgcc acattccagt aatcccaggt
     2041 ttcatcgcaa ttctcgcacg atgatgaaaa tcatacttct catattctga cttcaagcaa
     2101 ggtctaaacc ccaccaggct catttttccc attaggacat tataaaattg tggaaactca
     2161 tccaatgatg tagatcgaat aaattgtcca attcctgtct tctttgttcc atcaggaagt
     2221 attttattgc caatgattct tgggtcaaaa tccatcttaa acatgagatt attactcatc
     2281 ttattttctt tcatcaactc tgctttgcgt tcttctgcat ccatatacat actacggaac
     2341 ttatacatct taaatgtctt accattttga ccaatacgtt cttgtgcaaa gaaaatcggc
     2401 cctggtgatt tgatgtaaat aactggtgct acgaagataa aaataacacc tgttgcaata
     2461 catccaataa ttccagcaag aatgtcaatc gttctcttta taaatgcttg cctcatcgtc
     2521 ataacattaa tactagatgt aataacagta taatcaccaa ctcggccaat aaattgtttt
     2581 ccacgtgtac tatcagacac ttttgtaaga ttcatatgta cagcaagtcc catttccgaa
     2641 catctggcga ttaattcttt gggatatgga acactttcgt caacattgat gaatacttca
     2701 tctacccaat tctgtaaaag atattccgct gcattatcag catttgcaac tactgggaca
     2761 cctccgatgg tagttccata catatctctg tctactacaa tcacaccact aagtttatac
     2821 atgccataat tcttctcttt aagattacga actacatttt ttgcaatctt ttgagatgtt
     2881 acaataacta acgatacatc ttcaccgttt ttcatgcggt gatgcaacaa ccctttccaa
     2941 agtatacgaa ttatatatgt taataatcca tacaccaccc ccattataaa taaagaaact
     3001 ctggaataat catttccatc ctgcattgta ataaggtata aactgcacac aagtaacagc
     3061 ataaaagatt gctttataga agctacagcc tctttataat atcctctttt taaaacattt
     3121 ttatatgcct caaataaaaa tataatcgta agattctcaa gttctataaa aattgccatg
     3181 tggcgataaa taggaatcac atacggattc cagtcaccct gtcgcattac atatgatata
     3241 taaaaagcaa gctggacaca aaataagtct aataaaataa aatcaatatg tttagaccaa
     3301 ccacttgagt tttttctata catctctcac tctctcctca tttccttttt atctacgaat
     3361 aaagaactaa aataaaccaa acttcttctt ttttacctct tcatagattg gataattgat
     3421 ctcatccccg ttgatcagat cctttgcgac ttgtttcatc tgtactactt tttccttacc
     3481 aaactccttg gcaatctgct catagcactc ttttaagtaa aaggtgcgct tagagacccc
     3541 gtgtgcatca gaagcagcca tctgcacgag attatgtttg accattgttt tggctgtttt
     3601 ctggatatct ttgccaaata caccaacata acttggcgca gtaagttggg ccaaacatcc
     3661 catatctagg aacggaagca aatgatttgg gtcttctcga aaatgcgcgt tgcgttctgg
     3721 atggacgatc actggtatct ttcctagttc taacaactca aaaaaaagcc gttctgcata
     3781 taacggcact tccagtgtcg gaaattcgat cagaagatat gtatcatcta aatctgtaaa
     3841 taaaatttca tctcggtgga tgtcttcgat gagcgttccg gtaatacgta cttcttgtcc
     3901 ttccaataac gtcaaaggca actgtctttt ctctaattcg gcttgtaacg aagcgactag
     3961 agaaatgact tgtgattttt cattgctgta ttttccatta ttgtggtgtg gtgtgcataa
     4021 aatatgcgta atgccttgct gaatcgcttt ttcagccatt gcaatacttg cttctaagtt
     4081 ttctgccccg tcatcgattc ctggtaggat atggcaatgt agatcgatca tttctcttgt
     4141 ctcctctctt cttattcctt aatttccgta gtaataacca gcatctttgc tgtgttctgc
     4201 tccgttgtat acgacaccta agactcgtgc ttgtaccata tctaacagtt cttttgcttt
     4261 agtcaatgat tcttttcgtg acgtattttc ccgtacgacc aataaggtgc catccgtttt
     4321 tgaagacatg atctgtgcat ctgttacggc aacgactggc ggcatgtcga agatcactac
     4381 atcaaatagt tgtcttgctt ctgctaaaat ctgatccatt cttggcgaac ttagtagttc
     4441 tgatggattt ggtggtttag gtccacttgg cagaatcgac aaattgtcga ctggtgttcg
     4501 ttggatctca tctgctacac ttcctgatga actaagaaca gtacttaatc cgcttgcgtt
     4561 gttcaacttg aacgttttat agatgacggg cttacgtaag tctgcatcga ctagcagcac
     4621 ccgttgacca gatttggcaa aaacaacggc aatgtttgct gcagtggtcg atttcccttc
     4681 tccaggtcct gatgaagtca caacaatcgt tttgatttgc tgaccagctg cggaggcaaa
     4741 ctggatattc gtccgaatcg tacgatattg ttcagcgatt ggtgaagagg gattggtgag
     4801 tgtgaccaaa ctgactgcat gagtttgggc tgctttttgt ttctgtgttc gtgccatttt
     4861 ctttcctcct agactcttga gcggcttctg cgtgatggcg ctgttttatc gtttgtagct
     4921 tttggttgtg tctgagctgg tcctttttta cggataccag cattcaattc tttcgctgac
     4981 atttctggaa ctgttcctaa gattgggaat cctaatgtat ctgtcacata tttttcatct
     5041 ttcactgtcc gatcgagtaa ttccaataag aatgccagac ctacacctac catcattcct
     5101 aacattaagc cagtcattaa attcaattta ttatttggcg aaacaggtgt cgtattggca
     5161 actgcacctg aaatgatcga gattttatca acattcatca catcttttgc gttctcttgg
     5221 aaaacttgtg aagttgtgtt agcgatctgt tgagcagcca ctgcattcgt acttattgcc
     5281 tgaatcgaga acatttgtga attttctgcc tgattaactg agatggcatc tttgatttgt
     5341 tcagcagtca ttttcacatt gtcttctgtt tctaaacgat ctttgacttc attgagcact
     5401 aaatcactga cgatcatatc tttatacgta ttgatcattt gcaggttcgt attgacatca
     5461 ttgacatttg cagtgtctga ttgtggcagt gtcacgatca attgtgcttg tgaactgtat
     5521 tttggtgtca agatgaaaaa tgttacgatt cctgagatgg ccaagccagc aaacattgag
     5581 atgatgatta ttaccaagtg tttgcgtaat gtttcaaaaa tttctttcaa gctgattgtc
     5641 tcttccattt tttctccact ttcctattta ttctctagat tcatttgatc ttttaattct
     5701 tttttcacac gatcaagttc ttgatcactg actttttgat aggagatatt atcttgcatg
     5761 aagccttctc cttgcagctg atcttgctta atcgtaccaa atgcctcccg ataatctaaa
     5821 gcaatttttt tcatttcatc aaagttcatg tctgttttca tattcgactc tagagcattc
     5881 aaaacggatt gatagttgct gacaccgttt agactcaata cttttttcgc tacgccttct
     5941 acgatctttc tttggcgccc ttgtctaccg taatcaccat ttgggtcttc ataacgcata
     6001 cgagaataag ataatgcctg atccccgttc aatgtaattt taccgattgg aaagtcatag
     6061 ccatcttgtg agaaagtgag gtcattattg acttcgatac caccaactgc atcaacgagt
     6121 tcctttagcc ctttcatatt gatagagaca tagtgatcga ttgggatgtc tacatatttt
     6181 tgtacggtat ccatcgccat ggcagctccg ccgaatgcgt aagcgtggtt gatcttgtct
     6241 tttgtgtcac gacctacgat gtcgacatac gtatcacgag gaatgctgac gatcgttgtc
     6301 tgtttgtctt caggactgac tgtggcaacc atcatcgtat ctgatcttcc ttgatcgacc
     6361 cgacctaaat ctccagtatc aatccccatc aatagaacag agaaagattc ttgtttgtcc
     6421 agatcaactg gtttcgttcg ttctccttgg gttcgttcca ctgattcgta agttttctca
     6481 atagaatgtg agagatccag atataatttt gctcctactc ctacaattgc taatactaat
     6541 acgataagaa ctgctaaaat aatcagaaat atttttttcc ctcttgattt tcttcttcgt
     6601 cttgccatag tttcctccgt ttgctaacct tgcttgaatc aacgttttaa ctgtcaatag
     6661 cttatattgc tatagcccat ttgctcagca acagtttgtg cttttgttgc gtatgctgcg
     6721 cttatttttt cattccggat ctgctctatt tgactgagca ccgtcaaata ttgatcgtaa
     6781 gtagcggtgg cagtcacttg accatccttt aacatcgtat cgaataactg atctagttgc
     6841 gtgacttgcg cgatctgaat atcagcgaat cctagatatt gcataacaac cgttttccaa
     6901 ggactatctt taaaaaagct catattttca cgtacacgag cgacatctgc acttgtcacc
     6961 ttttctttga tgatgacatc gtctacagct tgctgccagt ttgatacatt tttagtaaat
     7021 aaatcgttga cagctgtctg gatcttcagt tttgattctg cactatctaa ctgtgtcacg
     7081 attgctttct tctcatcttg gattgcaaca gcttctttag gcaattctga ttctttgatg
     7141 tcgtaatcat ctgctgaaag tttgacagag gatagcttat ttgacaaact tgtgattgtt
     7201 tcttctgtta tttcagggtt tagaaaaatt ttttcttttg tactatacag cttgtctact
     7261 tcttttgata aagaagtcaa ggtgtctttt tgattggtga tggcagattc tgcataattg
     7321 atccgttgct gatgttgccg ctgattattg atatggtatc caaacaaaca gatcgcaata
     7381 gcacctgcac ctaccgccac agcagtcaat gacttgacga atggcttttc catagattca
     7441 ctcctaagta ttgatgatag ataaattaag atataattca cttacataaa aatgacagct
     7501 ttttatttaa aaaaacataa ttaaactaat atattcacag aaatatttgt a
//
LOCUS       DAPCMT010000130         7453 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPCMT010000130 DAPCMT010000000
VERSION     DAPCMT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.133"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            216..1208
                     /locus_tag="RD743_002317"
     CDS             216..1208
                     /locus_tag="RD743_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0564114.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1224..2108
                     /locus_tag="RD743_002318"
     CDS             1224..2108
                     /locus_tag="RD743_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0564115.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            2157..3764
                     /locus_tag="RD743_002319"
     CDS             2157..3764
                     /locus_tag="RD743_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0564116.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3765..5249
                     /locus_tag="RD743_002320"
     CDS             3765..5249
                     /locus_tag="RD743_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDU0564117.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            5426..6418
                     /locus_tag="RD743_002321"
     CDS             5426..6418
                     /locus_tag="RD743_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0564118.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            6470..7339
                     /locus_tag="RD743_002322"
     CDS             6470..7339
                     /locus_tag="RD743_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0564119.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         2385 a         1191 c         1646 g         2231 t
ORIGIN      
        1 tcaatgaggc cgcagaaaag ggtgcttaaa aataatctgt tgtttaaaat attattatca
       61 gaatacttag atacaaaatt ctggcacaaa gtaaaaaaaa tattgactta aaaaaatgga
      121 tatggtaatc tataaacgaa aatagtggta acgtttccaa aatgcatctt cgttaaattt
      181 ttattttttt aaacgaaaat ggaaacgttt ctaaaatgta ttgggaggag attgaattgg
      241 aaaagacatt gggaccaggt ggtgagataa gggcggtaca acgggataaa aaaaatattt
      301 cgaagtggga aaaattaaag aaacagttgc ggcgtgataa gtggatgtac ttgttgctct
      361 taccaggtat catcatgacg tttatcttta agtatgtgcc aatgtacggt gctgtgattg
      421 catttaaaga ctataatcct atgctaggaa ttttggaaag tccttgggta ggactagatc
      481 actttaggga atttatcagt tcaccaaatt ttacgctttt gctgagcaac acattaaaaa
      541 ttagtattta tggtttattg ttaggttttt ttccaccaat tattttagca ctgagcttga
      601 atcaaattat caataaaaaa ataaaacaga aaatccagtt gattctatat gcgccgaatt
      661 ttatctcagt agttattatt gtggggatgc tgtttttgtt cctatccgca aatggaccta
      721 ttaatgggtt agtagaaaag gttacggggc aaccaattat gttcatgtct aatccagctt
      781 atttcagaac gatttatatt ttatcgggaa tttggcaagg aatcggctgg tcatccattc
      841 tttacaccgc aacgctggcg ggagtcagta ccgatttata tgaggcagca aatatcgatg
      901 gggcaaatat tttacaaaag atttggcata tcgatttgcc tgcattgagg cctgtgatgg
      961 tgatcagctt catcttggcc gccggcggta ttatgaatgt aggatatgaa aaagcctact
     1021 tgatgcaaac ttcaatgaac atccctgcct cagaaatcat ttccacttat gtatacaaag
     1081 tcggcttgca atcaggtgat tatgcctact ctgctgctgt tggtctgttc aatatgatta
     1141 ttaatgtcgt tttattactg attgtcaaca atataaccaa gcggctaaac gatggtcaag
     1201 gcttgtaatg aaaggagaaa attatgaata gtgctttatc aaaaatcgac cgtcaaatct
     1261 tatggttgaa tcgagtgatt gtaacttttt tagtcgtcat caccattgtt ccgctgattt
     1321 atgtactggt agcttctttt atggacccag ctactttgat caataaaggt ataagcttca
     1381 atccgaaaga ttggacaata cagggatatc aacgggtatt tgcagatgat tccattttac
     1441 gcggttttgt aaattcgttg ttttactctt ttagcttcag cttattgact gtatttatta
     1501 cgatgatcac tgcgtatcct ttgtctcgac ctaacttggc ggggaaaaag attattatga
     1561 ctttttttgt cattacaatg ttcgtgggtg gtggcttgat acctacctat ttattggtaa
     1621 agaacttggg catgttagat agtgtatggg cgattatcgt accaggtgct ataaatgtgt
     1681 ggaatattat cttagcccgt acctatttta gtgctttgcc aaaagaactt tctgaagctg
     1741 ctactatcga tggcgccaat gacttgcaaa tactgttcaa agtcatgctt ccattagcga
     1801 aaccaattat gttcgtattg tttttatatg cctttgttgg gcaatggaac tcatactttg
     1861 atgccatgat ttacatcaaa gatcccgact tacagccgtt gcaactcgta ttaagaaata
     1921 ttttgatcca aaaccaacct tcacaagaca tggtgggagc caatactgcc atggcagaaa
     1981 tgaaacaaat tgctgagttg atcaaatacg caacgatcgt catttctagt ctgccattaa
     2041 tcataatgta tcctttcttc caaaaatatt ttgataaagg tgttttagtt ggttctgtta
     2101 aagggtaagt attgttcact atttgtatga ataaaaatta taaaggagat tttaagatga
     2161 aagcaaaatg gatgctgcca atcgttttta cttccgtact aattttgggg gcttgtggag
     2221 gctcaaaaga aaagagtaaa tcaagccctg attatgaact agaaaatgtc tcttttccat
     2281 tgaaagaaaa agtatcttta aaaatgatgt ctcaaagtgc ccctttggct cccaatgatc
     2341 ctaatgacaa attgattttc caacgattag aagaagagac caatctccat attgattgga
     2401 caaactacag tagtgatttt gccgaaaaaa gaaatttgga tatcgctagt ggtgatttac
     2461 cagatgccat cttcaacgcc gctgcaggtg actatgattt attaaactgg gcggaaagtg
     2521 gcgttattgt accgattgaa gatttgatcg aagattatat gccaaatctg aagaaaattt
     2581 ttgatgaaaa ccccgaatac cgacaaatgt ctactgcacc tgatggtcac atttactcta
     2641 tgccttggat tgaagaatta ggcgaaggaa aagagtcgat tcatacagta aatgggatgg
     2701 cttggatcaa caaggagtgg ttggataatt tgggtcttga aatgccgcaa accactgatc
     2761 agttgatgac tgtgttggag gctttcaaga cgcaagatcc aaatggtaat ggtgaagcag
     2821 atgaaattcc ttttagcttt attaacaatg gaggaaacga agacttaaaa tttttaatgg
     2881 gggcattcgg tctaggtgat aatgatgacc acttagtcgt tgataacgat ggtcaggtac
     2941 aatttactgc taacaaagaa gggtataaag aagccatcaa atatttcaat gaaatgaata
     3001 ataaaggtct tattgataaa gaggctttcg aacaagattg gaatacgtat gttgccaaag
     3061 ggaaagaaca taagtatggc gtgtacttta cttgggataa agcaaatatc acgggtgcag
     3121 atgatagtta tgacgtcttg ccagtcttag cgggaccaga tggaacgaaa catgttactc
     3181 gtacgaatgg catgggtttc tcaagagatc gtttcgtaat tactagtgcg aataaaaact
     3241 tggaacttac agctaggtgg atagataaaa tgtatgaacc gttacagtct gtccaaaata
     3301 actggggaac ctacggtgat gaagcacagg cgaatatttt tgaattaaag gatggtatgt
     3361 taaaacattt gcctttagag gggacagcac cgggggaact aagacaaaaa accgaagctg
     3421 gaggaccatt agcagttctt gattcttatt acggcacagt cactactatg ccagatgatg
     3481 cgaagtggcg tttagatttg atgcatgaca cgtatttgcc atatattaca gaagacaata
     3541 tctatccacg tgtcttttta gaaaaagaag aaacagatcg attagcaaaa atcgaaacgg
     3601 atatgtggga ttacatctat cgcaaaagag cagagtggat caccggcggt aaaatcgatg
     3661 aagaatggga agaatacctt gctgaattgg aacgtgtagg tttggaagaa tggattcaaa
     3721 taaaacaaac tggctatgat aattttttga agggagcaca ataagtgtta gtagaagaaa
     3781 cggcgatttt ggataaagca aatcgattta ttgaaatgat gcgaggaaaa gttaaccctc
     3841 agtatcgaca aaactatcat ttttccgcac ccatcgggtg gataaatgac cccaatgggt
     3901 ttatatatta caaagggcat taccatctct tctatcaata ctatccttac gatagtcgat
     3961 gggggcccat gcattggggg catgcgcgca gtagtgattt ggtccattgg gaagatctac
     4021 cagtagcgtt agccccagat caaccttatg acagcggcgg gtgtttctca ggaagtgcaa
     4081 ttgaaagaga cggaaaacta tatttatttt atacaggctg tcaagaaatt gatggtatta
     4141 gccatcagca tcaatgtttg gcggtctctg aagatggtat tcattttagc aaatacagtg
     4201 gtaatcctgt ggtagcagaa aaacagctag gtgaaaatgg gctaatacaa gaatttcgtg
     4261 atcctaaagt aattgttcac aacgatggtt actatatggt agtagctact aaatctacac
     4321 agtctactgg tagaatagtg atgtttcaat cgtcagattt gtatacttgg cacttttact
     4381 ctgtgctgct agaagggact tcagagcaag gaataatgtg ggaatgtcct gatttattcc
     4441 atttagacgg acgagatgtc ttaatcatgt ctccgataca aatcaagaag aacggactgg
     4501 aatatcataa tatcagttct accatggctt gtattggtga ggtaaactgg gaaacaggtc
     4561 agatgatagt tgataatttc catgaaatgg attttggaat ggatttttat gcaccacaaa
     4621 caacaagaga tcctaatggt ggacgggtga tgattgcttg gatgcagatg tgggatcgta
     4681 cattgcctac acatgagtta ggccatcttt gggcaggctc tatgagtttg ccccgagagt
     4741 taagagtgaa aaaaaatcga ttggtacaaa aacccatctc aactgtatat agtcatttag
     4801 tttatcaaca aggcagtgaa aatattgcag tgactgacac atcagtcgta tttcgaaacg
     4861 cgctgtctga ttcttcatat ttacatctag tcgcagattt ggagaaagct gaagttttta
     4921 caattgaatt actgcggtct aagacggatt gtctcaaact agcctatgac actgctacac
     4981 aggtttttga attggatcgt cagaaactag gaagagagct tacaggggcg gaagaagacc
     5041 ctctgaatgt acgaaaagtt gaagtacctt tgattgataa tcagctcgtt cttgagattt
     5101 ttagagatac ctcatcaatt gaagtcttta ttaatgggtt agaagttatg acggctactt
     5161 tctatgagct ggaaaaagct cgagatatct tgtttcattc gatcggagaa agtcgtattc
     5221 aagcttttga aacagcgagt gtgaaataaa tgtttgattg cttaggcgat gaatcaatta
     5281 tttaataggt tttgaaggat aatgttttta gaaaagaaca gatagctaga tttcccatgg
     5341 atttgctatt agtattttga aaaaattagt aatttatgac aaaattcatt atactaaact
     5401 tgtgcagaaa tgtgagggat caactatgaa agcaacaatg aaagatgtcg caaaattagc
     5461 aggtgttgga gtaggaactg tttcccgggt attgaataac ggttctgtta aagcttctac
     5521 tagagaaaaa gttgaagcag caatgacaga gttaaactat caaccagatt tttatgcccg
     5581 ggggttaaag atgaagcata cgaacacggt tgctttgatt ctacccacgg tatggcatcc
     5641 attttttggc gaatttgcct accatgtaga aacagcatta gaaagacaaa actacaagct
     5701 ttatttgtgt aactccagtg gtaatagtga aaaagaacaa gaatatatcc agatggtcag
     5761 ccaaaataaa gtagacggca tcattggaat tacttattca gatatcgatg aatacatttc
     5821 gagtaatctg ccttttgtta gtattgaccg ttatttcact gaagaggtga tttatgtgac
     5881 cgctgacaat gcggccggtg gacgtttggc tgcaaatgaa ctttatcgcc gaggatgtca
     5941 gaatgtagca tatatcggtg ggtaccaaga aacaccgaat gaaacgaaga gtcgtcgaaa
     6001 atattttgaa atcgagtgcc gtcaactggg gatttcctat aaagcgttag atatgttaga
     6061 acctttagac catctctcag aacgtgtaac aaattttttg ttagagaatc ccgatatcga
     6121 tgggattttt acgatcaacg atccaatggc attagctgtc attgatatgc ttgaaaaaat
     6181 agggaaaaac gtaccagagg atgtccaagt tatcggattt gatggtcaaa agatgacaga
     6241 aggagatcct tatctagtct caacgattgc ccaacctaca aagcaaatgg cggaagtggc
     6301 agtggcaaaa ttaattgctg tcattaatgg agagacagta gaaaaacgta ccgtattgcc
     6361 gattgaattt ggcgaaggtg acacaacgaa acctttagtg agaaacgatt ttgcttagtg
     6421 caaatacgtt tctttttttg aaaatgagtg aatgaaaggt gtagataaaa tgttatatgg
     6481 tggaatagaa gccggtggaa caaagtttgt ctgtgggatt ggtaatgaac aattacaagt
     6541 gatagaacgg aaaagttttc ctaccacaac accagaagaa acgatggagc aagtgtttga
     6601 tttctttgat aagtacaaag gaaatatcgc atcaattggt gtaggagcat ttgggcctat
     6661 tgatattcaa atcgaatcaa gaacttacgg atatatcacc aacacaccga agttagcttg
     6721 gcaaaattat cattttgtag gagctttgga aaacgccttg tcggttccca tctattggac
     6781 taccgatgta aatgctgctt gttatggaga atatatggca gggtatggaa aaggtaaaaa
     6841 tagtgttctg tattacaccg ttggtacagg tgttggtggt ggtgctgtag tggatgggaa
     6901 aatagtcgca ggttttagtc atcctgaaat gggacatatg ctcttacaac gtcataatga
     6961 agatcaattt gaaggaatct gtccttttca tcatgattgt ttggaagggt tagcatcagg
     7021 acctgcgatt gaaaaacgct ttggaagaaa agcccagact atctcatttg atcatcctta
     7081 ttggacgatt gaggcagatt atcttgctca gtgtgtgtat aacacaacgt tactatatgc
     7141 accggaagta ataattttag gcgggggtgt aatgaaacag ccgcaactac tcgagttgat
     7201 tcgccaatcg tttaaagaaa aaatgggttc ctacgtggat acgcctactc tcgaagaata
     7261 tctactaact ccaaggcttg ctgacaatgc gggcttgatt ggctgcctag ctttagcaat
     7321 aaagaaactc aatcattaga atagtattta ttagaactat tcataacata ccggaagttt
     7381 aaaagggtat tctgaaactt aaatagtctc aattcattct tctttcttga gaatctgagt
     7441 agagactttt att
//
LOCUS       DAPCMT010000131         7321 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPCMT010000131 DAPCMT010000000
VERSION     DAPCMT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.134"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(26..178)
                     /locus_tag="RD743_002323"
     CDS             complement(26..178)
                     /locus_tag="RD743_002323"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDU0564120.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(369..1247)
                     /locus_tag="RD743_002324"
     CDS             complement(369..1247)
                     /locus_tag="RD743_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0564121.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1286..2404)
                     /locus_tag="RD743_002325"
     CDS             complement(1286..2404)
                     /locus_tag="RD743_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDU0564122.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2401..4500)
                     /locus_tag="RD743_002326"
     CDS             complement(2401..4500)
                     /locus_tag="RD743_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDU0564123.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4551..5372)
                     /locus_tag="RD743_002327"
     CDS             complement(4551..5372)
                     /locus_tag="RD743_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0564124.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5369..6178)
                     /locus_tag="RD743_002328"
     CDS             complement(5369..6178)
                     /locus_tag="RD743_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0564125.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6199..6693)
                     /locus_tag="RD743_002329"
     CDS             complement(6199..6693)
                     /locus_tag="RD743_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0564126.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6705..7193)
                     /locus_tag="RD743_002330"
     CDS             complement(6705..7193)
                     /locus_tag="RD743_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0564127.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
BASE COUNT         2228 a         1688 c         1223 g         2182 t
ORIGIN      
        1 tattttatac ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta
       61 tgaagtcaac atttcgaaaa atgaacatac attggtaagc aactataact attaccacag
      121 acataaagta acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg
      181 tatacagcgc acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta
      241 atgaaaacaa cacgatttga cttagagcct ttttatttga attcattttc gctttatcca
      301 acttcatttg atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca
      361 ttcattaatt atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt
      421 attccggcat tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca
      481 acatatccat ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt
      541 ttcattacac caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat
      601 gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc
      661 tggcctttta cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa
      721 tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata
      781 tgccccattt ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct
      841 ccgatacctg ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca
      901 acatattcac catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa
      961 tattgcttta gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg
     1021 atatgaccga aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg
     1081 atgcctgcca attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca
     1141 actggagttg ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact
     1201 gcacagacaa atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc
     1261 cttcgaatat gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac
     1321 gtttagtatt tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt
     1381 gccgctcaaa taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt
     1441 cggtaaaaca gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt
     1501 cgtcaccaaa ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat
     1561 ctctatttga ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac
     1621 gtccgaactc atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg
     1681 ttgaggttac ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc
     1741 cccaccaata acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac
     1801 cctttaatcg atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc
     1861 aatttgcgtg aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg
     1921 tataaaaaaa tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg
     1981 tttggaaagg catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca
     2041 acgagttcac ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt
     2101 caacagcagg gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa
     2161 aactgccaaa ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc
     2221 cagtaatatc cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt
     2281 tgcggcttgg tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat
     2341 tttcgggagt gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac
     2401 tcatcttttt ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt
     2461 cgtatcactt attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt
     2521 acattcaatc gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt
     2581 atcaacagga tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat
     2641 cattaggctg acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac
     2701 gacttctcct tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc
     2761 tgccaaacaa attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct
     2821 cattaaaaca gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc
     2881 tttgatttcc tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg
     2941 atataaatga tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc
     3001 ttctccatca tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat
     3061 taacccttgc tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc
     3121 atcaccgatt ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca
     3181 aattgattcc tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc
     3241 tagggcttca aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc
     3301 ggtatgatac tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg
     3361 ttcccctgac cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac
     3421 ctgcggacac cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag
     3481 taacgcactg gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc
     3541 aggaaactta acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc
     3601 aaacatggtg aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc
     3661 aggatagctg ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga
     3721 aaagctttga taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag
     3781 ctgcccaaat agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga
     3841 ttcctgaaaa gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct
     3901 tgccacttcc tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg
     3961 ctgatccgaa tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc
     4021 gtgtgtttca ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag
     4081 tatgggagca aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg
     4141 caaacagtct tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac
     4201 aatcccgttt acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac
     4261 ccaaacacct ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat
     4321 cgtcccgcaa acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc
     4381 ctttacatca ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc
     4441 gtttaactca gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat
     4501 cttttccctc cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt
     4561 ccaagtaatg acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg
     4621 acttttttat ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta
     4681 aaaatactat cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc
     4741 aaaacggtat tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag
     4801 atactagcag cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat
     4861 ttagagccta atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata
     4921 ttgaacataa ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc
     4981 gctagtcccg cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc
     5041 aatggtccca tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt
     5101 tcagaatttt ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg
     5161 gtcgtcgaaa aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg
     5221 tagaaatgat taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc
     5281 ggattcaccg aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc
     5341 tcatctttag aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa
     5401 gcgttgcatt tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta
     5461 gatctctttg ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg
     5521 gtaactcgag atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac
     5581 tccaaagcat ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg
     5641 ttaatccgtt catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc
     5701 caaataaaac ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa
     5761 agaagtgcag attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag
     5821 cagccatcgg tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg
     5881 ctagtggaaa gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg
     5941 ctgtagccag cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga
     6001 cacccataaa gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca
     6061 tcaacgctcc tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca
     6121 tttcatgtcc agcaattcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa
     6181 tttttctcct tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat
     6241 cgccggtgta ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg
     6301 aatttctgac gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg
     6361 tgtcattttc ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc
     6421 aacttttacc gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg
     6481 cggatctttt aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga
     6541 tggtgcagcc atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc
     6601 tactactaac ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat
     6661 taatcgttca tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc
     6721 tcttcattta ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta
     6781 tttaccagtt cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc
     6841 agaattggca gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt
     6901 ttggtaatct tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc
     6961 tgctcgttca cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca
     7021 taagcattaa tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga
     7081 aagcctgaag ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct
     7141 ttctttctgt tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta
     7201 atcacgttaa ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt agacactttt
     7261 ttagacactt tatttttgaa tatacggtcc taataattca tagagcagcg aaagctcata
     7321 a
//
LOCUS       DAPCMT010000132         7176 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPCMT010000132 DAPCMT010000000
VERSION     DAPCMT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.135"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            179..1036
                     /locus_tag="RD743_002331"
     CDS             179..1036
                     /locus_tag="RD743_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDU0564128.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1029..1931
                     /locus_tag="RD743_002332"
     CDS             1029..1931
                     /locus_tag="RD743_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDU0564129.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1964..2314
                     /locus_tag="RD743_002333"
     CDS             1964..2314
                     /locus_tag="RD743_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDU0564130.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2363..3285
                     /locus_tag="RD743_002334"
                     /pseudo
     CDS             2363..3285
                     /locus_tag="RD743_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            3363..4256
                     /locus_tag="RD743_002335"
     CDS             3363..4256
                     /locus_tag="RD743_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0564131.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4294..4857
                     /gene="amaP"
                     /locus_tag="RD743_002336"
     CDS             4294..4857
                     /gene="amaP"
                     /locus_tag="RD743_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDU0564132.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4871..5059
                     /locus_tag="RD743_002337"
     CDS             4871..5059
                     /locus_tag="RD743_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDU0564133.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5072..5974
                     /locus_tag="RD743_002338"
     CDS             5072..5974
                     /locus_tag="RD743_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDU0564134.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6006..6248
                     /locus_tag="RD743_002339"
     CDS             6006..6248
                     /locus_tag="RD743_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDU0564135.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6504..6875)
                     /locus_tag="RD743_002340"
     CDS             complement(6504..6875)
                     /locus_tag="RD743_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564136.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2631 a         1210 c         1418 g         1917 t
ORIGIN      
        1 atacctttga ataaaaagcc atgaaaaacg aatagttagc gcttaccgat gcgttttgtg
       61 ataaaaaaat tttaaaatat tcaatgaaaa caaagcatat attttgcttt aaaaccaaat
      121 gaggaatatt ctagatgtag aacaggttag aaaaagatta gaaaaaggag tgattttgat
      181 ggagaagaga tacggcgtag ttcctgtggt aactgcttca gacgaaaatt atgctcctta
      241 cttgagcgtg atgatcgcaa ctgcattaga aaattgcaat aaaacaagac gcataaaatt
      301 ttatgtgatt gacgatggtc tatcagaata tagtaaagaa ggattggaag aaacggtaaa
      361 taaatattca agtaatgcaa gtatccaatt cctgacagtg gaaaaagata tctacgaaga
      421 cttcctagtc agtgaccata tcacaactac cgcttactta agaatttctt taccaaactt
      481 attagctaaa gaggattata agaaagtcct ttacttagat tcagatgtgt tagtactaga
      541 cgatatcgta aaactatatg atgagccgtt aaatggaaaa acaatcggcg ctattatcga
      601 tccaggccaa gtgaaagctt tggaaagatt aggaatcgat tccgatgact tgtacttcaa
      661 ttcaggagtg atggtgattg acattgatca atggaataaa aaagaaatta cagaaaaaac
      721 tatccattac ctcagtgaaa atggcgatcg aatcatctat cacgaccaag atgctttgaa
      781 tgctgtactt tatgaagatt gggaacaact tcatccaaaa tggaacatgc agacttcgct
      841 gatttttgaa cgtcatccag caccaaatga aaaatatgaa cgtcagtata aagaaggaaa
      901 tgaaaaacct tcgatcgttc actttacagg acatgataaa ccttggaata cgttgaaaga
      961 tcatccgtat accaatttat acttaaaaaa attagcacac agtactctaa cgaaagtagg
     1021 cgagatcaat gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca aaattcgtac
     1081 cacatttagc tgcattattt gtctcagtat tagataattg caacccgtct aaatttgttc
     1141 ggttctatgt gattgacgat gatatcgatt ttgaaagtaa acagctttta cgcttctcag
     1201 tgaaaaacgc acgaatgaac tcagatgtgg aattcttgaa aatcaataaa gaattcttta
     1261 caaatgtggt aatcagcgat cgtattccag aaacagctta ttatcgtatt gcgattccgg
     1321 aattgttccg cggcacagaa gttgaacgta tcttatatat ggattgcgac atgatcgctt
     1381 tacaagatat ctctaaatta tggcgtcttg acttcggtga ttcaatcgta gcagctgttg
     1441 aagacgctgg gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa tcaatgcgtt
     1501 atttcaactc aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa aatattacgc
     1561 aaaaagtatt agatttcatc gaacataatc cagaaaaatt acgattccat gaccaagatg
     1621 cattaaatgc tatcttacat gaccgttggt taccacttca tccgcgttgg aatgcacaag
     1681 gctatatcat ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt gaatacgaag
     1741 aaactcgcaa caatccttac attatccatt tttcaggaca tgtgaaaccg tggagcaagg
     1801 attttgaagg gccaacgaag aaatattacg aaaaatatgc agggatgacg gctttccgtt
     1861 gtgtagctaa attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa acagatgtag
     1921 ttgttaaata atagaagaca acaactgaag agggggagta ggaatggaaa ttacaatgtc
     1981 taaaaatgca gtagagacgt taattgaaaa ggttggggaa aatacaaaaa tcgcactcgc
     2041 gcttatcaat gattccgatc catttctaag ggacaaaggt gcttttgcaa aaggcagctt
     2101 ttttcaaatc attccttttg tttcggaatt tggagaatat gcgactaaaa tcgagcatcc
     2161 attattggac atctatacat ctaaattaga gcaaaattat ttcggtaaac gattgaacat
     2221 ggacttcaac aaacagcttg acagcttttc attagaaaat gaaatcgcag tactcgatta
     2281 taatatcaaa ttaaagaact gcttcttcag ctaatagttt cgagaaagtc caaaaaggag
     2341 gtatacattg agatactact ttgtggatga agaaaagaat gtaatagaaa cagatgaaaa
     2401 aaaatataat tggctcgtac ttgatagtgc aaagcccgaa gagattgagc aagcaatcga
     2461 agaattcgag cttcctgatg atattttcgt aggaaccgaa tatccggaag aagtttctcg
     2521 gatggagcgc ctttatgaga cgaaactaga gcatccattt tctttggtag tgattaatct
     2581 agatcagtca gataatcgta tcgaaaaaaa ttaacgccaa tatcttttgt actttccgat
     2641 aatctcttga ttacttgtat cgatagaaaa accgaatttg tggatcggct gctgacaaag
     2701 catggaaatc atttaaactc gtttgaaaag atcatcacgt atacattatt agatatttac
     2761 acccattacg tgaaagaact aagagagatg aaaaagcgaa ttgacgcttt ggataaagca
     2821 gctcgtaaaa caacggaaaa tgaagaactg tataaacagg cagatcttga aagagacatc
     2881 gtatatatcg atcatacctt aagagatcaa cgggaaacaa tggatcaatt atggaattct
     2941 gaaactttca tcaaacgttt agacgatgaa cagctactct atgatgtcca tttgagacag
     3001 cgacaaacag aaaaaatgat cgagatctat cgagatttat tagagagtat tggtgatcta
     3061 ttcaacggga tgatggacaa taatctaaat catatcatga aatatctaga ttcagctgca
     3121 ttaatcattt ccgttccggc gttgttcgca gggatttggg gaatgaatac aggaggatta
     3181 ccgggtaaaa gttctgctct aggctttata ctcgtagtct ttggctcttt gatagcagca
     3241 gtagttttag gttaccattt ataccggaaa gattatacaa attaaagaat ctatttaaaa
     3301 caggtatcaa acaaagaaat ttgttttggc aatacaattt aaaaactagg aggaatacag
     3361 caatgacaga accaaaatta atcgacccgc gaaacttgta ccatacggag aaatttccta
     3421 aacaagatca ggatacacca gctttacagc agaatatgat tcctgttcct gattgtggag
     3481 aagaatccta tgaaggcaat aatcaactag aaaaccgccg tgtactaatc actggtggag
     3541 attcagggat cggtcgtgca gcagcgattg catttgctag agaaggggca gatatcgctc
     3601 ttcaattctt cccaggagaa gaaaaagatg cagaggaagt agcaaaatac gtgaaagatg
     3661 caggacgtaa gattgtctta ctaccttttg atcttcgtga cgaacaagca cctaaagaaa
     3721 tcatagataa agccgtgagt gaacttggcg ggctagatac gatggtacta aacgcaggac
     3781 agcaaatttc ttgtcagtct attgctgagt taccacttga gcaagtcaaa gacacattta
     3841 tgataaacat catcgctatg tttgctttag tgaaagaagc tgttccgcat atcccagcag
     3901 gaggagcaat cgtaacaaca acttctgttc aagcattcaa tccaagcgaa catttattag
     3961 attacgcagc aacaaaagct tcaatcgcta actttacgat cggtttagca aaacagcttg
     4021 cacctaaagg aatccgtgta aatggtgtag cacctggtcc aatctggaca ccattacaac
     4081 tagacgaagg acaaccttct gattcgatcc ctgaatttgg tcagcaaagt ttactagaac
     4141 gtgcaggtca gccaggagaa ttagcaccag tttatgtttt cttagcatcg aataaagcaa
     4201 gttatgttac agcccaaatt tacggagtaa ctggtggaga agcgatcaac ttataagaag
     4261 ttgatcgttt cacccctatg aggaggtgcg aaaatgaata aaggtgtaaa aacgatcgga
     4321 gtgatcggaa cattactttt gctatctgtg cttttgtttg cgatactatt aagtgcaacc
     4381 tatccattgc cttttcagct agagaagttt cgattctttt ccatcactaa ttcatacgtg
     4441 caacaatatg tattttggct tgccgttgca tttgctattt tgacaattat cacgttactt
     4501 gttttcctgt tttatccaaa aacaatcgga aactttctac tgaaacatgg tgatggggag
     4561 ttgacgttgg ataaaaaagc aattgaagga atggttcgtt ctcacttgca tgaagatgaa
     4621 ttcatccatt caccaaaagt aaatgtcaaa gcaacgaaaa acaagatcgc cgtcaatgta
     4681 aaaggggagt tgaaacgaac ttcagcactc attggtaaaa caggaacatt gatgaaagaa
     4741 atcgaaaatg aagtacaaca catattggga acaaaagagc cagtgaaagt atcggtgaaa
     4801 tacacaggct ttgaagaacc agctaaacga agcaatgacc gcaaacgagt agaatagggg
     4861 gggaccaaaa atgaaagaac tattttcgac atacaagctc cctatcatct ttggggtact
     4921 tggattgata ttagccgttt tatggatcac cgtgggcttt ttcaagacct tattgatctt
     4981 gatcatggca gcgatcggcg tcgctatcgg tttttatctg aaacaaacga aaatcttaga
     5041 tcactatttt aaatcttagg aggaaacatc catgaataac aataacaaaa ataataacaa
     5101 taaaatgaac aaccaaaaac cagtgtctgc acatccagac ccaacgacac caaaccaatc
     5161 aaatggtgtg aacgatgaaa agaacaaaac agcaccagta tctgcacatc cagatccagt
     5221 agtccctaac gttcaacaag gagtagaaga gaagaaacat gatgcagaaa agcatgcacc
     5281 tcatcatcca ggaactccag aatctaaagt tccagaacaa catgatgcaa tcgatccttc
     5341 taaacctgtg gaagagcaaa aacatccttc tgaccatgcg aaagcacaag aaaaacttag
     5401 ttctgaccac acaaacggac aaggaaaact tggttctgat catacaaata accaaggaaa
     5461 gcatgattct gaacaggaag attcaatgcg cggtgagctg aattatgaag ataaagttgt
     5521 gcaaaaaatc atcggaatcg caattgaaca agtagacggt ttattatctg caaacggtgg
     5581 tttcttctct aatgtggcag gaaaacttgt gaatacagat aatgtaacgg ctgggatcga
     5641 aacagaagtc ggtaaaaaac aagtagcagt tgatatggat gtcattgtgg aatatggcaa
     5701 agatatcgaa aaaatctttg aagaaatgca agaagtaatc ggcaaagaag taaaaaatat
     5761 gacacattta gaagtgatcg aagtcaacgc taacgtagtg gacatcaaaa caaaagaaga
     5821 atttgaaaaa gatcaagaaa cggtgcaaga caaagtaact gacgcagcga aaaaaaccgg
     5881 tgaatttgct tcgaatcaaa cagataaagt aaaatcagca gctggaagcg gcgcacaaaa
     5941 agtaaaagaa aatacagaac cacgggttca gtaataagga agtaaaacat taggaggaac
     6001 gaaacatggg atttatttgg tcacttatcg tcggaggaat tatcggagca attgcaggag
     6061 caatcacgaa taaaggttct tcgatgggga ttattgcaaa tgtcgtagca ggtttagttg
     6121 gatcagcact tggtcaagca ttattaggtt cttggggacc aagtattgca ggaatggcaa
     6181 tcgtgccatc attgattggt gccatcattt tagtactaat cgtatcattc tttgctcgaa
     6241 aactgtaaaa agacttttaa aaagaggtcg tgaaaaagct gttcttcatc aagtaataag
     6301 aacagccaaa caaaaatagc ttctcatatt tttcatttgg ttgggcatat tatcgaagat
     6361 gcaagctttt gaacactgtt tatacggaat aagaggctgt gacaagactt ttgtcacagc
     6421 ctcttattta tgcgggcaaa ttcccaatta gatttttaaa agtttttcct taaatgtgtc
     6481 tttattgata cagagaagct tctttaataa aacaatgcct ccctaaattt tcttgagata
     6541 ggaaaggtga acatacaaaa taatacgacc actttgaaaa cccagatgat ccacaaatgg
     6601 atcgtatgat actgatgaag taaaaagcaa tggatcagaa actcaaagag attgaaaaag
     6661 acaaggaaga aataatatct cttttttact cttgtctgtt ttagccaata atagctcgta
     6721 aagtaatcta gacaaacaaa tagaagtaaa caaagtttcg taagagcagc aatcgtatca
     6781 tgttgctctg ctagacgatt gacgagcagg aaaaatgttg gtaatggaat atagatcacg
     6841 agtaatgaaa gaaaaaagta aacaataaat ggcatactat tcctcctaat tgtgtgagtt
     6901 cgccctcatt ttaaaataac gatatttgtt tgtgaagctt tttcgtgtcg taacaaatgt
     6961 tatctaatgt atttttaaat aaataatcat tattatcaaa ataatgttaa aaataaaaaa
     7021 gaattgtcca tcactgttat tcaaatcatt ttatcaaaac tacatagtaa atgttaaaaa
     7081 gcaacatgac ataatatata gaaggcttta cagtatatat gggagcttac taaaaaagaa
     7141 tggagtaaga gttaacacaa tgggccatta gctgaa
//
LOCUS       DAPCMT010000133         7141 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPCMT010000133 DAPCMT010000000
VERSION     DAPCMT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.136"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            336..809
                     /locus_tag="RD743_002341"
     CDS             336..809
                     /locus_tag="RD743_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDU0564137.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            806..2938
                     /gene="feoB"
                     /locus_tag="RD743_002342"
     CDS             806..2938
                     /gene="feoB"
                     /locus_tag="RD743_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDU0564138.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2940..3074
                     /locus_tag="RD743_002343"
     CDS             2940..3074
                     /locus_tag="RD743_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDU0564139.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3168..3812
                     /locus_tag="RD743_002344"
     CDS             3168..3812
                     /locus_tag="RD743_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0564140.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3999..5192
                     /locus_tag="RD743_002345"
     CDS             3999..5192
                     /locus_tag="RD743_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDU0564141.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5185..6912
                     /locus_tag="RD743_002346"
     CDS             5185..6912
                     /locus_tag="RD743_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDU0564142.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2314 a         1246 c         1591 g         1990 t
ORIGIN      
        1 agtcaaacaa caaacactac atattgtgtt gtggaaactg tggaatacta agttttgtgt
       61 ttttgaaaaa agagctgaaa acaagtatcg tgtgggaaaa tagcaaattt aatagagaaa
      121 aaattgtgga aaactatgaa gaaatatttt tttctcattt ttatctatca atactaaaga
      181 ttcgctcata atatcacaag tataattgac attctcaatt accgagagta ttctatgtga
      241 gaatgattct catttatttg cgtaacacgt aagaaaatta gaacatataa tgaatgattt
      301 taatacatga tttatttcac aagagaggaa tcaatatgca gaccttagcg caggcaaagc
      361 agaatttcgt ttatcaagtc gaacgaatcg aaacagaaga aaaaacaaaa cgccatttgc
      421 aaaatatcgg gatcgttgcc ggaagtaaaa tcgtcgtcgt caatttatca ggcaacaatg
      481 ccattttact ggtaaaaagt catcgagtgg cagtaagtcg ggaaatacta ggtcaaatct
      541 ttgtcaataa aaagactgct tcctctgaaa gctggacttc tcttgatcaa ttaaaagcag
      601 gagagactgg gatcgtcgta gcaatccacg gacaaggcgc agtgaagcga cggttaatgg
      661 atatgggatt gacgcgaaat gtaaaaatag ttatccgaaa actagcccct ctgggagatc
      721 caatcgaact gacagtgcga gggtatgaac tgacgttacg aaagtcagag gccgaactaa
      781 tcttgataca aagggaggaa aaagaatgaa tatcgcgtta gtcggaaatc caaacagcgg
      841 gaaaactagc ttgttcaatc tcttgactgg ttcgaatcaa tatgtaggga attggccggg
      901 cgtaacagta gaaaaaaaag aaggatcgtt gaaaaaaaat ccgaacattc atattcagga
      961 cctacccggc atctattctt tgtctcctta tacaccagaa gaagtgattg caagagatta
     1021 tttggttcag gaacagcctg aactgatcat caacattatt gatgctacca atttagaacg
     1081 aaatctttat ttaactacgc agttattgga tttagacatt cctatgatca ttggtctgaa
     1141 tatgatggat gtcatcaaaa aagaaggaaa aaaaatcaat ctagaaaagc tttcctatgg
     1201 tcttggtgta ccagttttac caatcagcgt attgaaaaag caaggaatcg aaaaactaat
     1261 cgaaaagatt caggagtctg caaaaaagcg gccttctatt cctgttccct gtacatatga
     1321 tccacgttta gaagcagctt tacatgaaat tgaagatgta ctaggagaac aaagcggcaa
     1381 gaaacgctgg tatgcaatga aatattttga acgagatcag aaggtaagag aggattacga
     1441 gatagcagtt tctcagcaaa aagagatcga gcaactgatc cagttaacag agaaactatt
     1501 ggacgacgat agtgagacga ttctggtcaa tgaacgttat gaatttatta cccaactatg
     1561 tacactctgt gttgtgtcaa atgattcttt ccaattatcg atgagtgaca aaatcgatca
     1621 gattgcaacg aatcgttggt tagccttgcc gatcttcgca tttgtgatgt ggctgatcta
     1681 ttatctggct attcaaacag taggaacaat ggggacagat tggatcaatg acacattgtt
     1741 tggtacatgg ttaccagaac atgttggcag attgttagct gaatggcaag tagcgggatg
     1801 gatgcaggag ctgatcttga acggcatcat cgcaggcgtg ggagcagtgt taggatttct
     1861 accgcaattg attatattgt ttttatgctt gagctttctg gaagactgcg gatatatggc
     1921 gaggatcgct tttgtcatgg atcgactttt caggaaattc ggtctttcag gaaaatcctt
     1981 cattcctatg ctgattgcta caggatgtgg agtgccaggc gtcatggcca gtcgcacgat
     2041 tgaaaacgag caagatcgtc gtttgacgat catggtcaca accttcatgc cttgttctgc
     2101 aaagttgccg attattgcct tagtagcagg ggcctttttt cctcatcaat cttgggtagc
     2161 tccttctgcc tattttcttg gtatgactgc aattattttc tctggaatca gcttgaaaaa
     2221 aacacgtttg tttgctggag atacagctcc ttttatcatg gaattgcccg catatcattt
     2281 tcctcaatgg tcagcggtcc taagacaaac ctatgaccgc agcaagtctt ttgttaaaaa
     2341 agcaggaacg attatctttg tttctagtat cgtgatctgg ttcagctctt cttataattt
     2401 ttatgctcaa cctgtttctg aagaccaaag tatcttagcc tttttcgggc gaatcctggc
     2461 agttgttttt cagccgcttg gttggggaaa ctggcaagga acagtcgcag cgattacagg
     2521 attggtcgct aaagaaaata ttattggcac atttggtatt ttgtttggtc atgcggaagt
     2581 atccgaaaat ggcagagaaa tctggcaagt gctacaacac acatatactc ccgcagcagc
     2641 ttattcattg ttagccttca atttactttg tgcgccttgt tttgcggcaa ttggggctat
     2701 ccatcgggaa atgaatgcca agaaatggac ttggatcgcc ataggctatc aatgcggttt
     2761 agcttattta gttagttttg tgatttatca gttgggacac ttattggagg gtggacaggt
     2821 agcgttaggg acgtatcttg ctattttact gatgatagga ctcgtttatg ttttgttccg
     2881 tcagccaaaa tccaagaacc agttttatac tatattgtca ttagaaggag aggaatagga
     2941 tggcgacatt cttattaagt gtgttaatct ttggaagtgc aggcgcaatt gtctatactc
     3001 gtttaaaatc cggtaagaat tgtgatgact gtcaaacagc ttgcccagta aaaaaggaac
     3061 agatcaagcg atgatcagtc gttgcaagag gctattctcc ccttttttgc ttattttgct
     3121 agagcatagt aagatgaggg aaagtaaaaa aagaaagagg aaagaaaatg tctggaaaga
     3181 aaaaaatcgt ccgcccgcgc taccagcaga ttgctgttga tttagccgaa cgtatcgttg
     3241 aaaaccgata taaagtgggt gagaagatcc atgcccgctc aacacttgcc agtacgtaca
     3301 atgcttctcc tgagactacc cgcaaggcta tcaatgtgct ggtagatctt ggaatcatgg
     3361 aagtacggca tggaagtggg gcatttgttg cttcgaagga aaaagcaaaa gatttcctat
     3421 tgcaatatca agatgtacaa tccatccaag atacaaaagc agaaatcatg gcaagtgtgg
     3481 agaagcaaca agatgagctg aatcattttt ctcaattatt ggatcagctg gtcaatcaaa
     3541 cgaagcgaat acataagatg aatcctttac ttccgtttga attatcttta tcagaaaaag
     3601 caatgcatct ggaccgttca attagcgagt tgaatatctg gcaggcgact ggcgccacag
     3661 ttatcgcaat ttctcataat gaagaactgc ttgtttcacc tggcccctat gctaaattta
     3721 cggcagggga catcgttcat tttattggaa atgaatatac actacagcgt atgacaaact
     3781 ttttttaccc ggagcatcag cttgcagaat gattgtgtga aatattaaaa aaatattaac
     3841 ttggctaaac cttttattta caaagattta gccaagtttt tttcagtttg gacaagcaaa
     3901 tttctttcat ttttgacaaa gtgactacat gatgttaaca tagtgtggtc acttttttca
     3961 gaaagaaaaa tgaatttaca taaaaaagga gaatttgttt ggtaaaagta gaagtaaaac
     4021 atttaacaaa gatatttggt aaaaagacac aagccgcttt agatatgatg aatgatcacc
     4081 agccgaaaac ggagatttta aagaaaaccg gtgcaacggt tggtgtgtat gacgtgaatt
     4141 ttgacgtaaa agaaggagaa atcttcgtga tcatggggct ttccgggagt gggaaatcca
     4201 cattgatccg tctattgaat cgtctgattg agccaacttc gggcagtatc tatattgatg
     4261 gagaagatgt atcgaagtta agcaaagaag aattgcgaga agtccgtcgt cataaaatca
     4321 atatggtttt ccaaaacttc ggtctgtttc cacatcgaac gatcttggaa aatacagaat
     4381 acgggttaga agtccgtggt gtacctaaag aagaacgaca agaaaaagca gaaaaagcat
     4441 tagaaaattc tagtttgctt tcatttaaag accaatatcc tagtcagtta tctggtggga
     4501 tgcaacaacg ggttggccta gccagagctt tagcaaatga tcctgagatc ttgctgatgg
     4561 acgaagcgtt ctcggctctt gatccgctga tccggcgaga aatgcaagac gaattactgg
     4621 acctgcaagc caatgttcaa aaaacgatta ttttcattac ccatgatttg aatgaagctt
     4681 tacggattgg ggacagaatc gccttgatga aagacggcga aatcatgcag atcggaacag
     4741 gtgaagaaat attgaccaat ccagcaaatg attatgtacg tgaatttgtg gaagaagtcg
     4801 atcgttccaa agtgcttacg gctcaaaaca tcatggtacc tgcattgacg acgaatatcg
     4861 aatctgatgg accaaacgtt gcattgacaa ggatgcgtaa tgaagaagtc agcatgctga
     4921 tggctgttga cagaaagaga cacctgaaag ggatcatcac tgcagatcaa gcgttagaag
     4981 ctaggaagca aaaacgtcca ttgatcgact tcctagatga aaatgtgacg gtgattggga
     5041 aagacatggt ggtaagtgat attttcaata ttatttatga ctcaccgact ccacttgcag
     5101 ttgtagagga tggaaaatta aaaggtgtcg tcatccgagg tagtgtgatt gaagcattag
     5161 cagaaacaag cgaggtgagt gaacatgaat aatctattga actatcaatt accagtggca
     5221 aactgggtag aaaatatcac agaatggttt acgacaactt tttctggcct attttcattt
     5281 ttacaaacga ttggacaggc agtgatgtcg gggattacaa atttactgtt agtgattcca
     5341 gctcctttgt ttatcttgtt attgacggta gcagctttct ttatttctaa gaaacgtcca
     5401 ggacttacgt tgttcacatt aatcggtcta tggtttattt ataaccaagg tctatggaat
     5461 gatttgatga atactgtcac acttgttctt ttatctagtg tgatctcgat catcattggg
     5521 gtaccattag ggatcttgat ggcaaaaagc agtaaagccc aaagtattat caaaccgatc
     5581 cttgacttta tgcagacaat gccaggattc gtttatttga ttccagcagt tgcatttttc
     5641 ggtatcggga tggttccagg ggtatttgcc tcagtcatct ttgctctgcc accaaccgtt
     5701 cgttttacga atttagggat tcgacaagtt cctaaagaat tagtagaagc ttctgattca
     5761 ttcgggagca caagtcgtca aaaactattc aaactagaat tgcctttagc taaaagtacg
     5821 atcatggcag ggatcaatca aacaacaatg ctgtcacttt caatggtcgt tattgcctct
     5881 atgatcggtg cacccggctt aggacgcggc gtattatcag cattgcaaag agcacaagtc
     5941 gggaatggat ttgtcaacgg ggttgcatta gttatcttag caatcatcat tgaccgtttt
     6001 acacagcatt tgaaccagcc aaacaataaa aaagctgcag gagcaacaac acagaaaagt
     6061 aaaaaacgtc aaggactgat cattggtgct gtagtagtag tgatcttagg tgcgattggt
     6121 atagggagtt tttcatctgc caaagaaaca aaacgtatca acttatctta tgttgaatgg
     6181 gatacagaag ttgcttcaac aaatgtagtc ggtgaagtat tgaaacaaat gggctatgat
     6241 gtaacgatga cgccattaga taattcaatc atgtggaaat ctgtttcaaa tggcgagtca
     6301 gatgccatgg tttcagcttg gctacctaaa actcatggtt cacaatatgc acaatataaa
     6361 gatcaagtcg aagacctagg tgccaatttg acaggagcaa aagttggttt agcagtgcca
     6421 gcctacatgg atgtaaattc gatcgatgag ttaaccgatc aggcaggtaa gaaaatcata
     6481 ggaatcgagc caggagctgg tgtagttacg gctgctgaaa acacgatcca aaaatatgac
     6541 aacttgaaag actggaaagt agagacatct tcttcaggag caatgaccgt tgcactaggt
     6601 caagcgatca aaaaacacga accaattgta gttacaggat ggacacctca ttggatgttt
     6661 gccaaatatg atctgaaata cttagaagat cctgaaaacg gaatgggaag cgaagagcag
     6721 atccatacga tggttcgaaa aggattgaaa gaagaccagc cagaagctta caaagtatta
     6781 gacaatttcc attggtcaga aaaagatatg gaaaaagtta tgttggaaat caacaatgga
     6841 aaagatccac agcaagcagc aaaagactgg atcaaagaga accaagaatt agtcgaaagc
     6901 tggaaaaaat aaaaagacag acacgaaata aatggagctt ccaaaattgg atcaaatatc
     6961 caaatttgga agctccatgt tcgtctctat cttttttgtc tataaaaaaa gagaatgcgt
     7021 agctcatctt cgaaacgtcc aaagacgccg acaaactatt atgaatttat gggtgcgagt
     7081 caaagagatc gatcgtaggt atgaaagact aatgtgatta aagtttatta tgtacgaagt
     7141 g
//
LOCUS       DAPCMT010000134         7011 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPCMT010000134 DAPCMT010000000
VERSION     DAPCMT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.137"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(60..653)
                     /locus_tag="RD743_002347"
     CDS             complement(60..653)
                     /locus_tag="RD743_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0564143.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(678..857)
                     /locus_tag="RD743_002348"
     CDS             complement(678..857)
                     /locus_tag="RD743_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564144.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(934..1443)
                     /locus_tag="RD743_002349"
     CDS             complement(934..1443)
                     /locus_tag="RD743_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564145.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(1539..1919)
                     /locus_tag="RD743_002350"
     CDS             complement(1539..1919)
                     /locus_tag="RD743_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0564146.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            2249..2998
                     /locus_tag="RD743_002351"
     CDS             2249..2998
                     /locus_tag="RD743_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDU0564147.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(3182..4459)
                     /locus_tag="RD743_002352"
     CDS             complement(3182..4459)
                     /locus_tag="RD743_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564148.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4538..5182)
                     /gene="pcp"
                     /locus_tag="RD743_002353"
     CDS             complement(4538..5182)
                     /gene="pcp"
                     /locus_tag="RD743_002353"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDU0564149.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(5381..6325)
                     /locus_tag="RD743_002354"
     CDS             complement(5381..6325)
                     /locus_tag="RD743_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDU0564150.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(6327..6983)
                     /locus_tag="RD743_002355"
     CDS             complement(6327..6983)
                     /locus_tag="RD743_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDU0564151.1"
                     /translation="MIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLELLGTSFV
                     NNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIFAAFNV
                     SFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQVLFIGG
                     AGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYYPGKGQ
                     "
BASE COUNT         2002 a         1480 c         1202 g         2327 t
ORIGIN      
        1 taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg tacatctttt
       61 tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg accgacaact
      121 ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc cacacttaga
      181 ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa taatcgcgaa
      241 ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata ccattcgttg
      301 ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata tttctctaag
      361 ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc gtaagcaact
      421 cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta acgataattt
      481 ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt ctgcttcaaa
      541 aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc tggagcatct
      601 tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa catgcagaaa
      661 aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat gaaataacca
      721 tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg catttcttta
      781 tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc gcaattaggg
      841 catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt agcttactct
      901 tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata cctgacggtc
      961 ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga ctagaagtgt
     1021 ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca tcgctgcttg
     1081 cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg ccttttccgt
     1141 gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt gtccttcaca
     1201 aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt caactgcaaa
     1261 atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg ctgctttttt
     1321 caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg cattgagcag
     1381 tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca tcattttttt
     1441 cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac gaatgaaaaa
     1501 tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag ttctaatggc
     1561 aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc ttctctaatc
     1621 ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat atcgtctact
     1681 ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg tggcgcatta
     1741 gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt atagtcatct
     1801 cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac ataaaaatca
     1861 cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt aaggttcata
     1921 tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt ccattgtaaa
     1981 tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg caaaaaaaac
     2041 agaaagtagg tcctccaaga cactcttcct gttcccaata taataaatga aagtaagaaa
     2101 ttatattttc aagaaaacaa acagaaaatt atcataacac aaattattta taattgaaag
     2161 actagttttt tttcaagaaa agcatacaat agattgtgtt agtaaataca gaacacacta
     2221 catttagtgt cttaaggagt cctcatacat gaagtcatat tttcagtttt tagctcatca
     2281 gctaaaaggc tggccgcaac aaaattatta tcttttcttt ttcagtcttg gctgccaggt
     2341 catgactctt gtcaatagtc ccatcacttg gctgtccgtt attacattta tcggtaccac
     2401 gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg
     2461 tattttgtct gcgatttgtt ttattatcgt tgggttttca gcaaagaact atttaagtat
     2521 cggtgaacag attgcttata tgatcacatt ggatatcccc gtattgttaa gtaaagaatg
     2581 gaatcataat atggcttcaa aaatccgaaa attcactgga aaaagctggt ttatcgcaat
     2641 catcagtact ttccttgtct atcttgtttc aggattcctg attggtcgtt atacagatga
     2701 tcctcggccg tgggtagatg ccattgcttt ttctattagc ttgactgctg gtatcatcag
     2761 tttcttgcgt tacaacaacc aatattactg gtggttagct tcagggcttg ctcaaatggt
     2821 cctttggtat atttcattta cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc
     2881 tgtttacttg ctgaatgatg tccttgcatt taccgtttca ccttggtata accaaaaaga
     2941 acgtgaacgt atgcaaaaaa tggaagaaga atactacaac gcacaacaac acgcataaaa
     3001 aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
     3061 cggcggaatc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
     3121 aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctctattt
     3181 tttatagtaa ttccttcaga tactttcttg cctcatcatt gaaacgcagt gattcttcta
     3241 acttctgata atattccaac gcttgtttct gagcttcagt aaaacgataa tgttttcctt
     3301 tccatttgtc gattgtctct tgcacatttc cagattggat atctttcact actagttgcc
     3361 atttttcttt tgagactgta cggtctttct tatcagcaga ttgtatagct tctcgttctt
     3421 tacgcaatac ttttaaaaaa ccatttctga ctatccgagg aatgctgtcg aagaaccctt
     3481 ctgtaaaacc atagcgacaa tattgcttcc aaataaatag atagattttt ggttggatga
     3541 atcgttggtt tttcgcaaaa tattccatct gagcagtgac ttctggtgtt cgttgacgtt
     3601 ttgtctttct caacacggaa ataaatgttt caaaataagc attaggatgt actggcactt
     3661 cttcatatac gatggattgc caatctttac gtaaaaatgc gcgatagttg atcaagaatt
     3721 cttctacatc ctttactaat tcttctgcaa agtcgtcaaa tagttctttt tttatctgtc
     3781 gcttttttga ctgcccattg atgtgttcac ttattttctt gatttcattt tccaatgctt
     3841 gataatccgc tatgtaaatc ggcatttcta cttggcgaaa ttctgcgagc cgctgagcta
     3901 attttatatc cggtaaatat ggctgtttca tccgacgatt ttggtacaag acaaaacagt
     3961 atttttgcca tagtccttca ggaaaatcga tattcttctg cttaagccat aataattcgt
     4021 ctaaggccag atcaactttt gtcattccct gatggatcga agctgccact gtcggagaga
     4081 catgcaggtg atcggcaaag tggttgatgc ctagttttag gatctttccg gtttttggcg
     4141 atttaacgat gtattgcact tttaattctc taccacactc acaatacaat ttttgctggc
     4201 ttcgttttgt tcgaaaaaac ggattgatct tgaagtcaga aaacacccaa tctgttgcag
     4261 ctaaataatg ttcttccata aaacgagagc tgattagata tcggcggtgc ttatcgacta
     4321 gttcccgctg ttttttcgtt aattttgcat agatctccca acgttgactg cttgtgtatt
     4381 gtttagaaat tttgatggga tagcttttgc tgatttcttt tggaaaccat tcttcaaaga
     4441 cggattgcca ttcttccacg gctgtttctt tcctttctaa ttacaaaaag ctggctacta
     4501 acataacgtt agcaaccagc cagtgtttct tgctaaatta gtgagttgtt ccggcatttc
     4561 cggaaatatc ttctttattt tctactgctg cttcaactgc ataagtcaaa gaagaagcaa
     4621 tgtcttctaa agacatagaa ggcgtaccca ctggacgatc gatcacttga tcgggtgaga
     4681 atggcacgtg gatgaaccca cctcgaattg ccgggaattg tgtttcgatc aaatgaagca
     4741 aacgatacat gatatcattg cagacaaatg ttcctgctgt ataagagatg tacgctggta
     4801 gaccatgatc ttgtacattt tttgtcattg ctttgattgg caatgaggta aagtaagcgg
     4861 ctggaccgtt ttcttctaaa gccgtatcga atggctgatt tccttcatta tcagggattc
     4921 ttgcttctgc tagattgatt gcgacacgtt cgaacgagac tgctgaccgc ccacctgctt
     4981 gtccgacaca gatcacgaca tctggttggt gctgttggat ggcttctttg actacttgac
     5041 tgcttttttc gaatacggtc ggtacttcca ctttgatgat ttcagcaccg ctgatttcat
     5101 ctggcatttt ttttactgct tcataggctg gattcacttt gtccccacca aatggatcaa
     5161 aacctgttac tagtactttc atctgttgtt cctccgtccg attgatttaa catatggtta
     5221 tcttttatca taccattttc cttgaacagc gtaaataaag gaaacaatag tttaaataac
     5281 ataacagatc aaacttaata tattcttata aaaaaatagt gttccactac aatgttagaa
     5341 gaggttgcga caattattct gtcacaaccc ctctcttcta ttatttaaat agaatcatat
     5401 aagcaatttg gaaaactagc ataaataacg caataaacaa ctggtttttg atcactccgt
     5461 atttgtcttt catctccagc atcgctacag gcacgatatt gaagttagca gccatcggtg
     5521 tcagcagggt cccacaaaaa ccgcaagtca atgcaaccat tcctattaac gctggattcg
     5581 ctccatgact aaatacgaat ggtgcaccga tcccaaccgt catcacggtg atggccgcaa
     5641 atgcattccc catgattacc gtaaatatca ccatgccaag agcgtaaatg atgatgccga
     5701 tgttcacatt tccttctgga acgatcgtac ctactgcatt ggaaatcact gtcccaactc
     5761 cagcgcttgt aaataccgca cctagagaag ctaaaagcat tggcaacatg cttaacggcc
     5821 ctactgtgcc cagcatatct gctgcatcat ctaaaaatgt acttggtcga ttatctcgag
     5881 aaaagaacat caagattagc atggctgcaa aaacaccgac gcccacgccg actaaagcac
     5941 ctaaattggt aaatagagca aacaagatcg caaagacacc gatactcaaa gctggaataa
     6001 aaattttcat tcctagtttg tctgacattt tttccatata ttcttttgat ggaagccggc
     6061 tttctccttt gctgactttc ttagcaattg ctggtatggt catcgcaaag atcaagccac
     6121 cgttcacgat tgccggaagc catcttcctc ctgcaataac tacccctaag accgtccaga
     6181 aaaaggctga cccataacga tgaggattgg ttttatctgt taaatttctt actccagtgt
     6241 aaatcaatac gagtcccatc acgatataaa ggatctccag taatttatcg cctaataaaa
     6301 tatcaggatt tgtaaaaaag tccatcttat tgcccctttc ctggataata tttcttcgct
     6361 aatttctttt ctttaagata gaagtagaca cttgccacaa ctaaagccgt gatagccact
     6421 ggaacttcca ctaaagcaag ctgtcccaat gttacgtcat atcctagatc tttcagtgtt
     6481 gattggacta gcaacgcccc tgctcctccg atgaatagta cttggccaaa gaaccagcag
     6541 acattttcca tagctgaagc catacctttt agttcttctt catgcttagg atggacttct
     6601 aacccttttg attcgatggt ccccaatgcc atcggtaaaa tgattggacg aacaaatcca
     6661 gcaactccac caaaactcac gttaaaagcc gcaaagatca ttcgaaagat cccataaata
     6721 tctacaatca taccagcaga tactttcttg aatcgcttga tcaaggaagc cgctgattct
     6781 ttcagaccat ttctttcaag cgttcctgtt gccaacataa tgatgataaa aatcgccatc
     6841 ccgcgattgt ttacaaaact tgtacctaaa agttctaaca ttccttgaat gccaagaccg
     6901 ccagtcaaag ctgtaacaac taaagcaacc attacgatca atatcgagtc taatttaaat
     6961 gcaaacccga gaataacaat caagacgcct aaaaggctaa gataattttc c
//
LOCUS       DAPCMT010000135         6887 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPCMT010000135 DAPCMT010000000
VERSION     DAPCMT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.138"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(351..836)
                     /locus_tag="RD743_002356"
     CDS             complement(351..836)
                     /locus_tag="RD743_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564152.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(975..1190)
                     /locus_tag="RD743_002357"
     CDS             complement(975..1190)
                     /locus_tag="RD743_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDU0564153.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1414..2214
                     /locus_tag="RD743_002358"
     CDS             1414..2214
                     /locus_tag="RD743_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0564154.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2233..4101
                     /locus_tag="RD743_002359"
     CDS             2233..4101
                     /locus_tag="RD743_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDU0564155.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4094..4585
                     /locus_tag="RD743_002360"
     CDS             4094..4585
                     /locus_tag="RD743_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDU0564156.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4573..5127
                     /gene="srlA"
                     /locus_tag="RD743_002361"
     CDS             4573..5127
                     /gene="srlA"
                     /locus_tag="RD743_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDU0564157.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5145..6140
                     /locus_tag="RD743_002362"
     CDS             5145..6140
                     /locus_tag="RD743_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDU0564158.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(6282..>6887)
                     /locus_tag="RD743_002363"
     CDS             complement(6282..>6887)
                     /locus_tag="RD743_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDU0564159.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         2370 a         1102 c         1371 g         2044 t
ORIGIN      
        1 ctaatacgat acaaaggcta tctttctaat ttgaaaaagg gatcattaaa ataataagaa
       61 gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc aaatatagaa aaattgaatc
      121 tagaaaaccg cccgacaata aatttcatta actcagttga ccgaaaatga agtagatacc
      181 aaaaatttat caagagagca agtagagaag tgagtatatt cataagagaa atagctgtaa
      241 cgaattatta ggttaatgca aatgcttatt tatactccgt ataactttct cagaaaaaat
      301 gataccaata atgacttgct agtactgtaa atccgtataa tatcatctaa tcaaaatggt
      361 gttccatttg catctgtcat cgtaatcgat ccttcgggtg acactcgata aaaaccaacg
      421 cttccagatc ctccttggga acggatttgt ttgctggaag ctgtgaacaa ataatctcga
      481 tcaatttttt gataaaagct aataacaata tcgtcacttt gtaattcgga acggtgggca
      541 ataagaaata gaagtgcttt gtcagagttt tctaatggaa gagaaggtac agtgctttct
      601 tgcatttcag atgttgaaga aatgctaaac gactgaatac ttgattggct atgttgcgaa
      661 tctacattac cagtagaagt attagtaatg gaagtagtag aagtactttt agttgaacca
      721 acctcagttg ttgaaatggc tgctgaagat tctttaacag aattctttgt tttatatcca
      781 caggcagaaa aaagtattac agttcccaac aagcctgcgt aaatcccttt atgcatgata
      841 gctcctccta aaatagatta tctttattca gtatatcact taaacaaaga aaagcagatg
      901 gtttaaaatg aaaaaacctt cccaataaag ggaaggttca tgaagataat aactttttta
      961 ataattatag ctttctaata tctttcatca acttttctag taatcattgg gaaaaatttc
     1021 aagcagtaga tcgcacaaat acctacgacg atataaatga tcttcgcaaa aatagttgtt
     1081 gaaccaccag caatcatagc tactaaatca agttcgaaca agcctaccaa cagccagttc
     1141 aaccctccca caatcaataa agttaatgca atagcatcta aagttttcat tagaattctc
     1201 ctttcttaaa tgataaaaca attatatatc aaaaagagaa acattgctta tataacgctc
     1261 aggaatttta gcaatctatg caaaaagatg aaaatttttt ttgctcttcc attagtacac
     1321 attttttagc tgaagctgta aatttcatgt cttaattttt tttagtgagc ttttatactg
     1381 gatttgtaag aaaaaagaag gagaggatca catatgtcgg attggttgga tattgcagga
     1441 aaagtagtaa ttgtaactgg aggttcctca ggaattggaa gaagtatcgt cgaaaatttg
     1501 ttgaagcaga atgcacaagt agcgaatttt gatgtaactg aatgccgtat acagcatgaa
     1561 aacctactat ctttaaaagt cgatgtgtct tctaaaacgg acataaaaga agggatatat
     1621 aaagtaatga aacattttaa gacgatcgat ggtctagtca ataatgcagg aataaatatt
     1681 ccttctcttc taatagatag aaatcatcca aaaagcaagt atgaattatc agaacaagtt
     1741 tttgataaga tgatcgctgt caatcaaaaa agtgtatatt tgatgagcca agcagttgga
     1801 agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc tttcttctga atcaggattg
     1861 gaaggatcag aaggacagag ctgttatgct gctacaaaag cagcgatgaa cagttttact
     1921 cgttcttggg caaaagagtt aggaaagagg aatgttcgtg tggtaggtgt tgcaccaggg
     1981 atactggaag aaaccggttt gagaacacaa gaatatgaag aagctctttc ttatacgaga
     2041 ggtataagcg tcgaacagct gagaaacggt tacagtcgaa caagtacgat ccctctagga
     2101 aggagtggta agctgcaaga agtggcggat ctagtctgct attatctttc agaaagatca
     2161 agttatatca ctggagtcac gactaatatt tcaggaggta aaacaagagg ataaaggata
     2221 ggggggacgc acgtgacgct agtaaacaga tggtaccaat tattgaaatt actagtcact
     2281 cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca aaaccagtaa tcaaaccata
     2341 aaaaaagata ttggggagtt gaatgaagaa attgccggta ttgcaaaaat catccaaaaa
     2401 aacaaacatt ttgaacttga aatttatgat tttgattttt ttgatgagat catgcaagga
     2461 aaattgaaaa cagccagtga tttcaattcg gcatccaaaa gaatcgctta tttactaaat
     2521 aggctgatcg aaacagagga tttcttacgt atggatgatt tgtctgaaga agtcggtgtc
     2581 agcagaggaa cagtggtcaa ggatctaaag acattaaaag agatgatcaa tgattttgat
     2641 gtaaagatta caggcacacc aaataaagga ctaagaataa taggagatga gctagaatta
     2701 cgactacttt tattttattt tgtttctccc tacttttcag agacgaaaac agaacagttt
     2761 ctagaagaaa aacaagcaat caaacatttt cagaagaaga cgaatgctag taaacaagaa
     2821 atcacccttt taacgaaagt tatcggtatt tctttgaata gaatctctag taactatctg
     2881 ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat atatccaaga attcaatgaa
     2941 ctgatcaatc agttggaaga aatctatgaa ctaactttga gccaatatga aaaagatttt
     3001 atcagttttc ctttgaacat catcaacaca agtataacga tgataaggaa agctaactct
     3061 gatttgcgct tatatttcga ccccatgatg atgcgtattc gacgattaga tctcattgat
     3121 ctggatgagt ccttcttata tcaagagatg aaacagcacc tctctttatt gataaatcgg
     3181 gtaatttttc gttatcgatt gactgattta ttttatggtg agatagaaaa aaagtatcct
     3241 ttttcttacc aaatcgctag cgaatgcata caagcattag aagctatctt gcaacataag
     3301 atctctaaag tagagatcag ctacctaacg ttgtatttgg agttagcatt tcgatatcaa
     3361 aaagagcatt tttcctctaa aaaagtagcc attgttcata cgacaggaaa aggaacagca
     3421 ttaatgatcc aaagacaaat caaaaggata ttggggaggg atatacaaat aacacatgtt
     3481 tcagaagaac aatatgaaaa agtagatcta aacaaatatt tcgctgtttt tacaacgatt
     3541 ccgttaaaaa atgtaaatga agagacaccg gtgatccatt tgactaatct ttttgatgac
     3601 tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc gattagccgg gtttcattct
     3661 actgtttttt tctatcaaat atggaaagaa aaaaagacgt atcaagaata tttaaacaaa
     3721 ttagtcgctc ctttgatcag aggctattat gtggatgaag aatttgaaaa aaggatcttg
     3781 aaaagagaat atcagcaaag cacatttgtt aacaaagaat ttgcttttcc tcacacaacc
     3841 aaccagctat ctcaacacat tgttttatca gtagggctaa taactgatca aggtttagca
     3901 gaaacaggag gaaatggagt tattttttta tttgccatac ctgaagaaat gacggctgaa
     3961 gttgagaatg aattatttga gctatacgat atcctatttg aactcataaa aatgggaata
     4021 gatgagttta ttgaattagc accgacacaa gaatcatttt taaaactaat caaaagtaaa
     4081 ggagagagac ttcatgaatg atatgtatct attcatagtt ttcgcagctt cagcttatgt
     4141 gctgcaagca gttatcagtt ggaagcaact aaagcatttc aatcaaacat atagcgaact
     4201 gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg aaaatccgtt caggcacgat
     4261 tgttatgcta gctatagacg atcatgatca agttttagaa gtcagaaaaa tgcaaggggt
     4321 tacatctttg gcaaaatttc attcattacc tgtctatgcg ggtgaagata tccattacat
     4381 cgatcgttac catccctcag ttcgagcgga agatcgtttg acacaagcag ctttagaaaa
     4441 tgccagagaa gtatacttac gaatagaggc aggaaatgaa aaaagtgaac aaccttcatt
     4501 gccttttatt gctatgctca ataattttcg gatatggaaa agtacgatca aagaaaaaag
     4561 gagtgacaaa cgatggatat cataacaaaa tttgctgagg gatttatgag gctgttccaa
     4621 acaggtgcag aaacattcat aagctggatg acaggaatcg taccagttgt cttgatgctg
     4681 atggttgcaa tgaatacgct gattgctttt ttaggtgaag accgagtaac gaaagtagcc
     4741 aaattatcag cgaaaaatcc gcttactcgt taccttgtgt taccgttctt atctgctttt
     4801 atgttgggaa atcctatgtc atttacaatg gctcgtttcc ttccagaata ctacaaacca
     4861 agctattatg ctgctcaagc tcaattttgt catacgagca atggtgtatt cccccatatt
     4921 aatcctggag aattgtttgt atggatgggg attgctcaag ggatcgaaac cttagggcta
     4981 aattcgatgg agctggctat ccgatacttg cttgtgggac tgctgatgaa ctttattggt
     5041 ggttggataa ctgattttac tactggattt gtatgcagac agcaaggaat cgttcttagc
     5101 aaaaaagtag agctttcagt tgattagaga aaggagagtt ttaaatgtat ggtgatatta
     5161 agatcgtaag aggaaacggc gggtatggag gtcctttgat tattaggccg acaaaagata
     5221 aacacaagtt catctatgtc acaggtggag gtgagaggcc aatgatcgta gataaaatag
     5281 aagaactgac aggtatggag tctatcaatg ggttcaaaac atcaatacct gatagtgaaa
     5341 tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg tggaatttat cctaaaaaag
     5401 ggataccaac catcaatatc gttcccacag gaaaaagtgg cccattagca caatatatca
     5461 ctgaagatat ttatgtttca gcagtagggt tagaacaaat acagccacta atggaaactg
     5521 atgataacaa agaaacagta aatactgcat ctccagataa aaagtacaaa tatgatactt
     5581 ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg gatagcaaaa attggaatgg
     5641 gcgcgggaaa agtagttgct acattcaatc aatcagcaag agaagcagta gatacgatga
     5701 tccggacagt gatccctttt atggctttcg tttccctttt gattggtgtg atacaaggtt
     5761 ctgggatcgg taatgtattt gctaacatta tggcaccttt agcaggaaat gcagttggat
     5821 taacaatcat tggctttatt tgttcactgc catttttatc tcctttgctt ggtccagggg
     5881 cagttattag ccaagtgatt ggaacattga tcggtgtaga gattgggaaa ggaaatattc
     5941 ctccccaatt agcattaccg gcactatttg ctatcaatac tcaaaatgca tgtgatttta
     6001 ttccagtcgg tctaggtttg gaagaagcaa aagctgagac agtagaagta ggtgtaccat
     6061 cagttcttta ttcaagattt cttaacggca tcccacgagt tatcattgct tgggtagcaa
     6121 gttttggatt atatagctga gtttagtcta atggtgataa gtcaagatga aatttaagat
     6181 tttctttatt gagtaatatg ttttgaagcg cacgaaacta gacccgatca aaatcaaatt
     6241 tttttcgggt tgattcaagg atttgcgtca ataacggatt gttatttctc tatcacactc
     6301 cttggtggcg tatagagttg gtgtttacgt agtagcgtat ccaccagacg cgtaaatttt
     6361 cttgcggtta ggacgagtgc acgtttgtgt tgatgctttg gaacctcctt atatttactt
     6421 tgataaaaag ctttgtattc aggaagataa ttttttacag agttggcagc ttcaactaag
     6481 tagtatctaa aatatcgatt tccttgtttt gtcagaggtg tattttgcga ctgatagttt
     6541 ccagattggt tcactttcca acttaatcca gcgtattttg ccaattttgt ctgatcttca
     6601 aatctttcga tttgaccaat ttcagcgatt aaaccggcag catagacttt accaacgcct
     6661 ggaatactgg ttaagcattg atattctgga atgacaacaa ttacttgttc gatggcttta
     6721 tctaattctt tgatattttt ttcaagtgct cgtatttctc gtacgagaac acttaaaaca
     6781 acattaattg attcttgtgc aagagcacct aggcggtaac tcatggtaat tgcacgttga
     6841 attgctttag cgattttttc tggttgcttg aaacggcctt ttccttt
//
LOCUS       DAPCMT010000136         6840 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPCMT010000136 DAPCMT010000000
VERSION     DAPCMT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.139"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(139..321)
                     /locus_tag="RD743_002364"
                     /pseudo
     CDS             complement(139..321)
                     /locus_tag="RD743_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            308..505
                     /locus_tag="RD743_002365"
     CDS             308..505
                     /locus_tag="RD743_002365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564160.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(548..1123)
                     /locus_tag="RD743_002366"
     CDS             complement(548..1123)
                     /locus_tag="RD743_002366"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDU0564161.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1278..1804)
                     /locus_tag="RD743_002367"
                     /pseudo
     CDS             complement(1278..1804)
                     /locus_tag="RD743_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2261..3772
                     /gene="gadC"
                     /locus_tag="RD743_002368"
     CDS             2261..3772
                     /gene="gadC"
                     /locus_tag="RD743_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HDU0564162.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3785..5185
                     /locus_tag="RD743_002369"
     CDS             3785..5185
                     /locus_tag="RD743_002369"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDU0564163.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5350..6790)
                     /locus_tag="RD743_002370"
                     /pseudo
     CDS             complement(5350..6790)
                     /locus_tag="RD743_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2119 a         1177 c         1227 g         2317 t
ORIGIN      
        1 aattttgatt ttcaataaaa atatattaca ataataatcg taatagctaa tgaaacaatt
       61 tctcaatctt tttaccactc caaacaggga gcgtcaataa ttttgtgtaa ataactcgtc
      121 cttctgcaaa ataatgggtt actcagtaaa cattgaagct aatgtatccg tcacttgttg
      181 gaagccttta tggcttctat ttaagaattt ttgattgtat gtatcaaaga tggatactag
      241 gaaacgctct agtgattctt cattttgaaa ctgctctttt ctgcggctgt atttcttgat
      301 ttgtttattg aaagattcga ttgtagaacc agaaggaatg gagaacactt ttgaacttta
      361 caacaaaact actgttgata aattaattcc aattttagtt ccaactgatt taaatggtca
      421 tgatgtttta cttcgtttta acggattttt aggtgaggat tatagatacc taaaaatttc
      481 tgatcctcaa cctttaacag aataataaaa aacgtgtaat caattatttg attacacgtt
      541 ttttatttca atcttcgttg tagtagtggt tgcgacatac cgaaaggtaa tcttcattac
      601 ctcctactac aatctgttca cctgaataaa ctggaatacc atcaacaata cgagcattca
      661 ttacagcctt tttattgcaa aaatggcaaa tagttttaat ttcctcaatt ttatcagcaa
      721 atgtcaataa tgcttcggat cccttgaaga gattattttg aaaatcattc ttcaatccat
      781 agcaaattac tggtacttcc tcattcatta cgataagtga tagatcaata atattctctc
      841 tacttaaaaa ttgagactca tcgaccaaaa tacaatcaac ttttccgtac tgatcaataa
      901 tctccactaa ttctccgtac acatccattt cagaagtaat tgcaatagct ggtttcgaaa
      961 cacctatacg actctcaact tttccaaatc ctgatcgagt atctattgcg cttgtcatta
     1021 tgataactct cttcccttgt tcttcataat tatgagccac tttcagtagc tcaatactct
     1081 ttccactatt cattgttcca tatctgtaaa ataattgtgc cattaaattt attcccttct
     1141 tttacaattc aattattcct taattccatc tgcttttatg cagctgctcg tttattcctt
     1201 gatatgtcta taacatctac tatccctaaa accaatttat aagccattag agatggtatt
     1261 cctgaacatc tctcatattg ttttttgagt ggtatatcat ctgaaaaact gaaggaatca
     1321 tgaaaatagc gacatactta tcatttagag ttctaacttt aaatgcattt acttcttcag
     1381 tcagcacttt agtcatgttg gacatggttt ggggcgtgta gtgatgccca tacattttct
     1441 caatcaagtc tgcgatttcc gacatggtaa taccttttcg gaagaggtga atgacggtct
     1501 cctctaatgt gtcattcgtc cgtctataag caggaacagt ctgttgcttg aactcgccat
     1561 tgcggtcgcg cggaatctgg agatgaagtt ccccatactc ggtcttgacc gtacggtcat
     1621 aggagccgtt acgagaatta cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga
     1681 ggaaagccgt taactccgtt ttgagaagcg tattgacagc tttttccaaa tggacacgga
     1741 ataatccatt caaatcgcct ttgttcgcta gagtctttag gatttctgta gtaaaatcgt
     1801 tcatatggaa gtcctctttt ctgtgaatgt gttgtggtaa ctctattcta cagaagggac
     1861 ttcctttttt ctatttacac aaaatatttt acactctcaa caaaagtatc ttcgattaat
     1921 acttgctcaa gttatgtgca gctacgaaaa agtaggtacc gaggtacttt ctttttcgca
     1981 gctttttttg gacttatgag aagtacattc gctggaagaa tacgatataa aacgagcttt
     2041 ttcactaaag atattaatag agtgaaagaa acaagctttt gttgtcagtt ttttcacttt
     2101 tctaaaaatg aaaataaaaa attctggaag tggagaagaa ttttgcttgc tatactttgt
     2161 cttgtagaaa agaggtagac aaaataatgc aagctcaaat aagcgatttt ccataacctt
     2221 ggtaggaatg acaaattaaa taggaaaggg aagtaataat atgaataaaa aaaagttatc
     2281 tctattcggt ttttttgccc tgacagcttc catggttttg actgtttatg agtatccgac
     2341 ctttgcaaca tcacaattac atttggtttt ctttcttctt ttgggaggta ttttatggtt
     2401 tctaccagtt gctttatgtg ctgcagaaat ggcaacagtt gaaggatgga aagatggtgg
     2461 gattttcagt tgggttagtc aaactttagg agaacgtttt ggttttgcag ctattttttt
     2521 ccaatggttt caaattacag taggttttat tacaatgatc tacttcatac taggagcgct
     2581 gtcgtatgta attaattttc cagagcttaa tacagatcca tttatcaagt ttattggtgt
     2641 attagtcatt ttctgggtac tgactttttc acagttgggc gggaccaatc gtaccgctaa
     2701 aattgctaaa gcaggatttg tcattggaat tgttattcct tcgatcattc tctttggttt
     2761 agcagcagcc tactttatcg gcggtaatcc gattcaaatg gctattaatg cgaaagcttt
     2821 tattcctgac ttttctcagc tttcaacttt agtagtattt gtatctttta tattggctta
     2881 tatgggcgta gaagcttcag catcacatat taatgagctg gaaaatcccc aaagaaacta
     2941 cccattggca atgtttttac tagtgatttt ggccatcgcc ttagatgcaa tcggcggttt
     3001 ctcagtagca gcggttattc cacaacatga cttgtctctc agtgctggtg taatccaaac
     3061 atttgaaacg cttattttac attttaacag tcatttacat tggttggtaa aagtgattgc
     3121 tctcatgatt gcctgtggcg ttatgggtga agttagttct tgggtagttg gtccatcacg
     3181 agggatgttt gcagctgctc agcgaggtct gttaccagtt ttcttccgga aaactaataa
     3241 acaagacgtt ccagttccgt tgattatgat acaaggaatt atcgttacta tttgggcagc
     3301 agtcttaact tttggtggag gaggcaacaa tttatcattc ttagtagcaa tctccttaac
     3361 tgttgtcatt tatctcgtag gatatttact atttttccta ggttactttg tattagtttt
     3421 taagaaaaaa gacttaaaac gaacttacaa tgtaccagga aaaactgttg gcaaaacgat
     3481 tatcggtgga attggtttta ttttatcagt gttcgcattg cttatctctt ttgtaccacc
     3541 agcttcaatt gcagctagtg agcaccgaac ataccaaatc atactactta ttagctttgt
     3601 agtgactgtt cttttacctt ttatcattta tgaagtacat gataaaagtg ctcacacaac
     3661 tattgaggaa cctactcacg tgaaagctga agacgttaat cccgctgttt atccagctgc
     3721 tagaggagag caccacgtta caaaaaatga tgaacatatt ttaaaacatt aggaggaatt
     3781 cattatgtta tacggaaaag ataatcaaga agaaaaaaac tatttggaac caatttttgg
     3841 ctctgcaagt gaggatgttg acttgcctaa atataagtta aacaaagaat ccattgaacc
     3901 acgaattgct tatcaattag tacaagacga gatgttggat gaaggaaatg cgcgattaaa
     3961 cttagctact ttttgtcaaa cgtatatgga acctgaagca gtgaaattga tgacccaaac
     4021 gttagaaaaa aatgcaattg ataaatcaga atacccacga acaacggaaa ttgaaaaccg
     4081 ctgtgtaaat atgattgctg atttatggca tgctccaaat aatgaaaaat tcatgggaac
     4141 ttcaacgatc ggctcttcag aagcctgcat gctgggtggt atggccatga aatttgcttg
     4201 gcgtaaacgt gctgaaaaat taggtcttga tattcaagca aaaaaaccta acctggtgat
     4261 ctcttctggt taccaagttt gttgggaaaa attctgtgta tattgggatg tggaactgag
     4321 agaagtccca atggatgaaa aacatatgtc aattaatcta gatactgtca tggattatgt
     4381 ggatgagtac acaattggta ttgtaggtat tatgggtatt acttacactg gtcgttatga
     4441 tgatatcaag ggtctgaatg atttagttga agctcacaat aaacaaactg actataaagt
     4501 atacattcat gttgacgctg catcgggtgg cttttatgca ccatttactg aacctgatct
     4561 agtttgggat tttcaattga aaaatgttat ctcaattaat tcttcaggtc acaaatatgg
     4621 tttggtatat ccaggtgtgg gttgggtctt atggcgtgac caacaatact taccagaaga
     4681 attagtattt aaagttagtt acttaggtgg agaaatgcca actatggcta tcaacttctc
     4741 tcatagtgca gcacaactaa ttgggcaata ctacaacttt gtacgctatg gctttgatgg
     4801 ttatcgtgat attcaccaaa gaactcatga tgttgctgtt tatttagcca aagagatcga
     4861 aaaaactggt atttttgaaa tcattaatga tggatcagaa ttgccagttg tgtgctataa
     4921 gctgaaagaa gatcccaatc gcgaatggac actatatgat ttatctgatc gtctgttaat
     4981 gaagggatgg caagtcccag cctacccatt acctaaagac ttggatcaat taattattca
     5041 acgcttagtt gttcgagcag actttggaat gaacatggct ggtgattatg tacaagatat
     5101 gaaccaagca attgaagagt tgaataaagc tcatattgtt tatcataaaa aacaggatgt
     5161 gaaaacgtac ggctttactc actaaaattc ttcagagaaa tataaaagaa gttaattaac
     5221 taataaaagg agttgtgatt accacacagc tccttaaatc tatttgaata aatatatgat
     5281 attcatttgt tacactctca atcttagctg ttgttctatt catcattact cttattgtaa
     5341 gtagttcagt caaatttgct tgttcaattg atcgataaca gtatgaattg tttgaaaagt
     5401 aatcggatat ataattgttt tctcactacc taagccttct tggtataaag aactatcgca
     5461 aagtataatc cgctgagtac aaaagaaagt gtcgtctact atttctctaa gttgtttaca
     5521 aatgatatta ctaatagaaa agtgttctcc caaaatgtct tctaactgtt gtcgatagta
     5581 taaataccta aagtctgacg gagctactat gaacacttca attggagatt tccgtttatg
     5641 ctcatttaaa agacttaaac taatggttag tttacgaata gaattctcat tccaacgcag
     5701 aaataaattg ttttgttttc gccattgttc aagaatttca tgaacttcta agtacaaagc
     5761 gcgttgctct tcatttaaca agtaagtctt atcaggttga aatatctgaa tatcagccca
     5821 aagtgagcgg ataaacggga gaagtgcttt atcaaaaagt atctgatttt cttcagatat
     5881 attcagagat aagataagtt tttgactgag ctcaatcacc aaagggttat ctgctacatg
     5941 gttttgataa acaacgttca agtctttttg taacaattca aagttatcgc atagataatt
     6001 tctagagttg aatagaaagt agatataaag gcattcatct tcgtcatgac ataaagagaa
     6061 agaaagttgc tctaatcctt tctgtattag ctgaaacaag ggagtatgtc gggcaactct
     6121 tttttcttcc tgactaaaat atattgggtt agtcttagat ctctgcaaac cgatagcgat
     6181 acctaataaa ataaattgtt tttcaactgt agggtaactt cttgaaaaaa aatgtttttc
     6241 tacataatca atcaattgtt gaccgctttg aataattgat ttatttagct ggatcaactc
     6301 tgtccgattt gtctcataga ttatcgttag taacaaactt cgataatcct tttcaggaat
     6361 ttcaaaatgg ccatcagcat ttaagtactg catttgttta aaaaaattaa tcaactcatt
     6421 tcgtaagttg taaactttgc ttaacgaaat gaaggtagta tcagctatat tactccaatt
     6481 gtgttcccct gataaataat gtcctagtac attgaaaaag tcagagcgct gatagatgag
     6541 ttgagtgagt gccaacctcg aaaacgaaga gtcgaatgcg actgtaaagc tacttctaga
     6601 tttctccact gaaataattt gctgatggag tatgtcggta atgcattcca agtcgttagt
     6661 gacagacagt gagtgggcaa acgttcaatt gatctgctat ttcgcgaatc tgaatggtcc
     6721 ctttttcgtg taattgctca caaatgtaaa cttgacgaaa aatacttttc tccagatagg
     6781 tttccaacat agtactcctc ctagtcattt tatacataac tattataact ctaccaatcg
//
LOCUS       DAPCMT010000137         6813 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPCMT010000137 DAPCMT010000000
VERSION     DAPCMT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.140"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(83..1819)
                     /locus_tag="RD743_002371"
     CDS             complement(83..1819)
                     /locus_tag="RD743_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDU0564164.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1816..2520)
                     /locus_tag="RD743_002372"
     CDS             complement(1816..2520)
                     /locus_tag="RD743_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0564165.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2651..3154
                     /locus_tag="RD743_002373"
     CDS             2651..3154
                     /locus_tag="RD743_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDU0564166.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3114..3452
                     /locus_tag="RD743_002374"
     CDS             3114..3452
                     /locus_tag="RD743_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDU0564167.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3473..4891
                     /gene="ffh"
                     /locus_tag="RD743_002375"
     CDS             3473..4891
                     /gene="ffh"
                     /locus_tag="RD743_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDU0564168.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            5003..5581
                     /locus_tag="RD743_002376"
     CDS             5003..5581
                     /locus_tag="RD743_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDU0564169.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5585..6106
                     /locus_tag="RD743_002377"
     CDS             5585..6106
                     /locus_tag="RD743_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDU0564170.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6185..6739)
                     /locus_tag="RD743_002378"
     CDS             complement(6185..6739)
                     /locus_tag="RD743_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDU0564171.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         2195 a         1282 c         1421 g         1915 t
ORIGIN      
        1 agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg atgagtcatc caactttaaa
       61 acgcacgagc agttgctctt ttttacattt tcggaatggt aacggtgaaa gtcgaacctg
      121 ttccaagatg actgtctaca gtaatcgtac cattaaggag ttgcacgtag tcttttacaa
      181 tcgctaaccc taatccggtc ccgcctgaat gtcggcttcg cgccttgtct acgcggtaaa
      241 aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc tataccaaag tcttggacag
      301 aaaacgatag atcatctgtt gcctgatagc ggataataat ctcgctgtcg ctttttgaat
      361 attggacagc attttcaatt agatttttga taattggata aaggatatct gttttagtag
      421 taaatggttc gttctttcca atcaatcgaa tcgtcaaccg tttcttttcg atgattggct
      481 gataattttg aacgatttgc tggaaataag gttctgtata aattgtctgg atctcgtaag
      541 aattctcgcc atcttttgac agctggatga tttcccgaat cagtttatct aaccggatcg
      601 catctttttg catgatctca agaaaagaag tcagagtttg cgggtcatct tttgcaccat
      661 ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat tcgtgagaga
      721 cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt aagtcatacg
      781 ccacccctag aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa ctcaagtcta
      841 atgtctgccg accttctgta agggtgatct cctgatggat cagcggatgc tcaggagtca
      901 cttgatgaat cagctgaatg agttttggct cttgaatgac ttctgtgtac cgctgttcag
      961 gttgatagct taccacgttt aaatgtagct gcatctttgg attaagcagt agtaaacgag
     1021 aatcagcatc atcaatcagg aatacgccaa tcatcagttc gttcagtaac gtatataatt
     1081 gctcttctgt ggtagtacac gcccgataca tcttactcat ttgttcgctc aacgcattaa
     1141 tcgtctgata caattcttcc cattgatcgg gtgtttgcat gattacttcc gcctgattag
     1201 gattttgcac catttttttt agtacgggca gcacggtttt caatggctgg tttttttgat
     1261 agatcagata atagatcatt cctgcaagaa caaggaagaa agacagaaag aagaaaaaga
     1321 cccaacggcg aaaactctca gttctcggta aaaaaccgct tgtcggttct gcgatacgta
     1381 aaatggcttc taactggcca ctttttttga ctggtaatgc gacatataaa agatcctcat
     1441 tcaaagtagt acttttgcgc aaagcgctac ctaggttccc accattcaat acggctttga
     1501 tttcgggacg ggagtttcgc tgttcattca atgtttcatt gtttgtgtca aataagatct
     1561 ttccagtagc atccattaaa gtaatccgtt cgttcgactg atggacgaat tcttccaaag
     1621 cagttttgtt ttgcgctgct tggaggtttt cgacatctaa ttgccggatc agcagttgtg
     1681 ctttttttgt caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa taatgggaaa
     1741 tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag agacgttcat
     1801 aaattttttt ccatttcatc gtttgggttc ctgaaactta tagccaaatc ctctaatagt
     1861 caccaagtac tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat gactaacgtg
     1921 tacatctacg attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt
     1981 gtctcggtcg atcacacggt cttttcgttt catgaaataa acaagtaatt caaattcttt
     2041 cggtgttagt tcaatcagat ggtcgtctac ggtcacttga taattggtca aatcagcagt
     2101 gatttgtcca atcgatagat attcaggctc agcttcatct tttgattgct tacgaggttc
     2161 aattcgtctg aatattgctt tcatacgggc taatacttcg cgaggactaa atggtttggt
     2221 caaatagtcg tcagcaccta tttctaggcc aatgatccga tccacttggt catctttagc
     2281 cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc agccgctgag tgatctccat
     2341 gccatccatc gttggaagca tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc
     2401 taattctaat ccttcgcctc catctgtggc actggttacc ttgtagcctt ctttttccaa
     2461 attgaacgtc aagagcgtta caatagaagg ttcatcatca acaactagta tttttttcat
     2521 tcaaatctcc tttaaatagg tttcattctg tttatatttt agcatagaat cagtacaaag
     2581 ggctatttta caaaagaaat aaatggtata ataggaaggc actttgctga atagaaagga
     2641 agagcagtca atgattggaa tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg
     2701 acagaaaaaa gaagtaccag aattgatgga actagttgaa actggagaag ctgtcatggt
     2761 ctgtccagaa gtgatcggcg gattaccaat tcctagagat cccgctgaaa tcattggtgg
     2821 aaatggattc gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt
     2881 aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat
     2941 tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga acccattcga tttatgacgg
     3001 aagtttttcc ggaataaaaa aaaatggtgc cggagtggct actgcttatt ttattcagca
     3061 aaaaatgact gtgctctctg atgaagaatg gctgaaaact agaggtgagc agaatggaaa
     3121 tcgaaaagac caatcgaatg aacgcgctct ttgaatttta ttcgacctta ttgacagaaa
     3181 aacaaatgaa ctacatggaa ttatattatg cagatgattt ttcattaggt gagatcgcgg
     3241 aagaatacga agtaagccga caagcagtat atgacaatat caagcggaca agcaagatct
     3301 tggaggatta tgaaaaaaaa ctccatcttt tttctaatta tattgttcga gaacaggtat
     3361 tggaaaatat gactgcatat attacagata agtacccgga agataaaaaa ctacttgaat
     3421 atgtacagca aattcaagca atcgaagaat aaataaaagg aatgaagaaa atatggcatt
     3481 tgaaagctta actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt
     3541 atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac tagaagccga
     3601 tgttaacttg caagtagtca aagattttac taagcgcgta cgcgaacgtg cggtaggggc
     3661 agaagtgctg gaaagtttat cacctgcaca acaaatcgta aaaatcgttg atgaagaatt
     3721 gacgatcaca cttggttctg aaacagcaga gttaaataaa tcaccaaaaa taccaactgt
     3781 tatcatgatg gctggtcttc aaggggcagg taaaacgacc tttactggta aattagccaa
     3841 ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg tatatcgacc
     3901 agctgcgatc gaccaattga aagtattagg tcaacagcta gacgttcctg tatttgatat
     3961 gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg atggagttag caaaagaaaa
     4021 gaaaaacgac tatgtattga tcgatacggc aggacgtctg catatcgatg aaacattgat
     4081 ggatgagttg aaacaaatca aagaattgac gcaaccaaat gagattttgc ttgttgtcga
     4141 tgccatgact ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc aattaggtat
     4201 tacaggggtc gtcatcacta aacttgatgg ggatactcga ggcggggcag cactatcgat
     4261 ccgctcagtg acaggcgcac caatcaaatt catcggttct ggtgaaaaat tgactgactt
     4321 agaagtattc cacccagatc gtatggctag ccgtatcctt ggtatggggg atatgctgac
     4381 attgattgaa aaagcgcagc aagactacga cgagaaaaaa gcggaagaac ttgcacagaa
     4441 aatgcgggaa aattcattcg atttcaatga ctttattgaa cagcttgatc aagtgatggg
     4501 tatgggccca ctggaagatc taatcaaaat gatcccagga atgaatcaag tccctggaat
     4561 cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg tttattctat
     4621 gacgccagca gaaagagcaa atcctgatct gttgaaccca agccgtcgtc gccgaattgc
     4681 tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc
     4741 aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac atcccaggta tggaccaaat
     4801 gtttggttca ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa
     4861 aaataagaag aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg gtacagactg
     4921 aaaatgtcta ctgccggagc ttttctatca gcaattttta ttctttacta atacaattac
     4981 tctaaacgaa gaggtggcac agatgagatg tccgtggagt gaacaaacag aaagtatgcg
     5041 gcaatatcat gatactgtct ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa
     5101 attgatgctt gatattaatc aagccggatt gagctggcag acgattttaa ataaaagtga
     5161 agcattcgat gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac
     5221 aaaaatcagt gaactaatgg aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc
     5281 agccgtccaa aacgccaaag cagtcttgaa aattcaagaa gaaatcggca gtttttcaga
     5341 gtatctttgg tcgttttcta atggaaaaat cgttgattct tcttatcatt cacaagaaga
     5401 agtcccaaca acaagtcctc tttcagatga attagcaaaa gatctaaaga aaagaggatt
     5461 taaatttatc ggaagcacaa caatctatgc ttttttagaa gctgtcggga tcatcaatga
     5521 tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata
     5581 aagcatgcga aaaatcatat tttatggtgc tataagttta gatggctatt tggcaacgaa
     5641 aacggatgat cttcaatggc tttttgacac tccaacaggt aagcaaacta cttatgaagc
     5701 tttttatgaa acaatcgata cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa
     5761 atatttggag accgaagcaa tttatccaga gaaaaagaat ttcgtttttt ccacccacgc
     5821 acaattacaa ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa
     5881 tctacaaatg acagaaggaa aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc
     5941 gctattggaa catcaaatga ttgatgaatg gtatatccag attacccctg tattattagg
     6001 ggacgggatt cctttattcc aaaaaggaac atatgaagcg cgttttgaaa tgctagacac
     6061 gacacgattc ggtgaattta tcgaattgca ttatgtacga aagtagaagt aaataaaata
     6121 aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg ccttttttat taccgttatg
     6181 ctttttactc tgttaatttg ttagaaaaat ctagatacgc atctaaaatc gtttggaaga
     6241 aacttaatgt tccttcaacg atattaccat tttcatcaat tagattcgca acgtttccga
     6301 tatatgcttc cggttgctgc aaggttggca cattcaaaaa gacaagtgat tggcgtagat
     6361 ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc agtaacgact aaacctggtt
     6421 tatgatccca aacactgtgt ccataaggtc ttgagcctac atctaaagca tttttcaata
     6481 ctgctggcac agaacggttg tattcaggag taacgaaaac gacaccgtct agtcccctca
     6541 cttcctcacg aaaacgagtc cattcagcag gagcctgttc tggagtttcg agatcctcat
     6601 tgtaaaatgg cagatcatcg atttttacaa atacactttc gtacccttca ggaagcaatt
     6661 tcgcaaaagc ttctgctact tttttgttgt aagaatcttt tcttaaacta ccaataaaaa
     6721 atccaatttt ttttgtcata cgatttttcc tccttcaaac tatctataat aatcttacaa
     6781 aagataagta actggagcaa ataaaaacac tta
//
LOCUS       DAPCMT010000138         6567 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPCMT010000138 DAPCMT010000000
VERSION     DAPCMT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.141"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(7..1164)
                     /locus_tag="RD743_002379"
     CDS             complement(7..1164)
                     /locus_tag="RD743_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564172.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="RD743_002380"
     CDS             complement(1167..1523)
                     /locus_tag="RD743_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564173.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="RD743_002381"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="RD743_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDU0564174.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="RD743_002382"
     CDS             complement(2991..3830)
                     /locus_tag="RD743_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDU0564175.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="RD743_002383"
     CDS             4015..4902
                     /locus_tag="RD743_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDU0564176.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="RD743_002384"
     CDS             complement(5486..6181)
                     /locus_tag="RD743_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDU0564177.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="RD743_002385"
     CDS             complement(6165..6563)
                     /locus_tag="RD743_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDU0564178.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2065 a         1301 c          966 g         2235 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catctct
//
LOCUS       DAPCMT010000139         6486 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPCMT010000139 DAPCMT010000000
VERSION     DAPCMT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.142"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..894)
                     /locus_tag="RD743_002386"
     CDS             complement(<1..894)
                     /locus_tag="RD743_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDU0564179.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALS"
     gene            1531..1617
                     /locus_tag="RD743_002387"
     CDS             1531..1617
                     /locus_tag="RD743_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDU0564180.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(1738..2427)
                     /locus_tag="RD743_002388"
     CDS             complement(1738..2427)
                     /locus_tag="RD743_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564181.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2434..2712)
                     /locus_tag="RD743_002389"
     CDS             complement(2434..2712)
                     /locus_tag="RD743_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564182.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(2672..3586)
                     /locus_tag="RD743_002390"
     CDS             complement(2672..3586)
                     /locus_tag="RD743_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDU0564183.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(3568..3948)
                     /locus_tag="RD743_002391"
     CDS             complement(3568..3948)
                     /locus_tag="RD743_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDU0564184.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(4398..4685)
                     /locus_tag="RD743_002392"
     CDS             complement(4398..4685)
                     /locus_tag="RD743_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDU0564185.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(4705..4929)
                     /locus_tag="RD743_002393"
     CDS             complement(4705..4929)
                     /locus_tag="RD743_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDU0564186.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            5061..6140
                     /locus_tag="RD743_002394"
     CDS             5061..6140
                     /locus_tag="RD743_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0564187.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         1752 a         1334 c         1232 g         2168 t
ORIGIN      
        1 cgaaagtgcg gactctgttt gtttttcaag agcttttttc tctttttcta gttgtttgat
       61 ttgttggagg agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa ttgttgtagc
      121 gggttcttcg tattcttttt gtttatcagc taaagccaat gctttatttt ggtcttcttt
      181 ggttggtgca tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga acgtaaattg
      241 atagccaata acaggttttc cccgtccttt tccgtatttc tttttgattg ttagtccttt
      301 gaagattgca gttagttctt gacgtatagg ttttataact cgtttatcta tatcaggttg
      361 cttataactt ttaggaatat ctaataattc aagaaagtct tctttggaga aatatgcgaa
      421 acctttggtt ctgtattgct ttaacagacg aaacatagtc tttgcatagc cactttgtag
      481 ctcgttgaat tgttgtaaag aatagcgtac ccactcgtct aaattattta acaaaggcag
      541 tgctttttcg taaattcgta tatctacgta aggattttct acgtccccgt tgattttaaa
      601 ttctgtaaac attacaaaca tatcgtaaga aagcccagac tcacttctac gaccaaagcg
      661 taaagataag attttttcat aagttgcttt taaatcttct acaaatcgta catttgcagt
      721 tgctttgtag ttacttaaat cttttagttg gtcaaacgta aaacgaactg tcttatcccc
      781 tttttcacgc attctagaca cgatggaaaa aaacaaattc atttcaaccg gtgtaaattt
      841 tcttaaagga attgtattta attcatggtg gtattttaca atctcattag ccatataatc
      901 acctcaatcc aattatgaat gacatttttt aaatgtcaag acattcatat ttgacgttca
      961 aacagacaaa atgtccttca taaacagaca aaatgtcctt cataaacaga caaaatgtcc
     1021 ttcataaaca gacaaaatgt ccttcataac attctgtatc cattggtaga gtaaggacac
     1081 agcttacggt aaagaggttt aaaaagaggt ttaaaaagag atataaaaag aggggggcta
     1141 attttcccgc ctaaaaacca cttctttttt ctttaaacag gttcgcgcat ttttcaaaac
     1201 tgccaatcca aaaccttgct tcctacggtc gccaaaaagg tgtgatcgct acgctctcac
     1261 attgtactgc gcagtcgcct aaaggctcct ttgctaaaaa catgttggta aagaggcctt
     1321 tgagcggttc tgaatgctta tctaagccgt tttaagacag tcttggataa aaatccttat
     1381 ctagctttaa agcgccttac aggcggtcag ggcaagccct aacaacccta aaataagcga
     1441 tacttaaaaa caccacacaa gtttaatttt ttaagatata ataacttgtc gcctatccga
     1501 cagtcttgcg actggaagga ggtgtatcta atgtttgaat tgttcatttg cccccttctt
     1561 atagaagttt gtgtctcttt attcagttat tggttgaata accgaaacaa gaagtaaaat
     1621 caataaaggc gacatcaacc tgaaaaaaag ccccagagaa tccgacctct ctggggcttt
     1681 cgtgtatcta atgtttgatc atttgcatgt ttagtttaac atgattgccg atcaaattca
     1741 attttgctgt tgctgatcta accattcctt gacacttttt agataattta atgccgtttc
     1801 ttttttcgtt ggattttgta tgttcgttgc ttgctgaagc agtgctttat aacgttcgta
     1861 ggtaactgat tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag ggattgtatc
     1921 actgaataaa ttataacaag cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt
     1981 tttggttcgc tcatagttgt cttgaacctc tttttgctgt ttttctggca agttttgata
     2041 aagacgagtg agtttttcca tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat
     2101 atcgttcatt tcctcataac aagtggtaaa ctcttccatt ttttgtgtta acacttgctc
     2161 ctttttcttt ttatcacatg ctgcttcata cttttgagtg atatccccta ataaaatact
     2221 tcttgaaata cgtttatttc cctctaaaac gccgaataaa ccacttaaaa cgattgataa
     2281 tgtaaatata acccctggca ataaaaacct cttaaatcga attttagagc cttttaatga
     2341 atatcttgtt tgataagcct tatagctaag ccaaataatt cctaaaatac tggcaaaaat
     2401 gagtagatag ggtaaataga atgccatttc tatttattct ccccatacca gccaaaaggc
     2461 taacagtgtc atgattaagc aacataaggg ggcaagaata tccattgatc gctgcataat
     2521 tacttttttc tgttctttat ttcgctggtt taatagcgca tggtggaatt ttttgacttg
     2581 tgaatcaatt gtagtttgta ttctttcttt ggtataaggt gcaagaaaag cacgttgttt
     2641 ctttatttca ttttctatgt cttgtaacgt tttagaacga taattcttgt tggtagtctg
     2701 ctcctctttc aacgtgttgg tcatggtttc ttcgagtcgg tctaatttcc ggttgttcag
     2761 tcgtaagagt tcgttgttct cttcgtactt cttttgcagt ttcgcgaatg ctagctcgag
     2821 ctcttcgtag ctcgttggtt cgttgttgat tgtaggctcg ttctcgtctg tccagctcat
     2881 gtaaaatcgt ctccttttcg taatcctttc ctagtttgtt ggatcgtact tttttctttt
     2941 tgtggatcaa ttcgtaagta aaatttttcc cacgattcaa gcaatgaatg ccgaatgttt
     3001 taagagaatc acaaaattgg ttatacgttc gagttttttt gtttttcatt gtttgatcaa
     3061 tcgcaaaccg aatttcgtct ttccaaacag gttcattttt ttcttttaat ttgatttcag
     3121 acatggttcg tttttcgggt ttcttccctc gttcagtgat tgttaagtca tgttcaagac
     3181 aaatctcgtc atttaggtca taacatttgt ttaaaaatgc ttgaccatgg gcttgaaatt
     3241 tgttcccagt ttccagattg acggagttaa tgacaatgtg gttatgaata tggtctttgt
     3301 ctgtatgtgt gtaaatcgcg atttggtggt caggggctat ttttttcgca tactctgccc
     3361 ctagttgatt ggctttttca gcggtaattt ctcctggtcg aaaagactgg atcaataagt
     3421 gtgcttgtac acgatcgtct tttccataca gcatgcgaat ttgtttcatt tcgcttttgg
     3481 cataatcaat gtcacagttt atccctgttt tgacggttgc tcgtggttcg gcgtaattga
     3541 tcgctcggct acaagaagtg gttcggctta ctttaactgt tgccatatct tttgtagctc
     3601 cttttgcatg atttttaggt tttcggataa gcgttgggcg tcgccaggat caacgtcgtg
     3661 ttggtgtgtg ttgcaccact tggcaatttg attgatattc ccgccagctt tggctaaatc
     3721 gcgcgcgatt gcctgagcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg
     3781 tgccttggct ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag
     3841 ctttgcaaat tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt
     3901 atatttttta gaatgattgc tagccaaata ttgattttgt tcgctcataa gtcccaccct
     3961 ttcagtacgt cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaataa
     4021 gtaaagctca ctaaagctca ctaaagggct tggcaagctc tatatttgct agccaaatac
     4081 ctacgtattt ggtgcaaata accgcttgtc aaaggggcgc aagctccccc ttgaatcccc
     4141 ctccccccca ctctccacaa tacaaaaaag actagcaaat gctagccact ttttgtaaag
     4201 tttcgcttgt ttcaccttcg gtgaaattgt tgcaaatcaa taattaggta gagctacatt
     4261 tttttcgttg ttcaaagatt cttatgccat ttactattaa taaatctata aatatgataa
     4321 tttttcggct ctataattta tgggactgat gaatcagaat tgattcttca gtcccatttt
     4381 cattttattc caaagaatta actccaaata ccaatagaag cccatcctct atatacttga
     4441 gattccttac taacaatttc ttgtaattct attaaagatt taggtaatcc aaatttatgt
     4501 tttaaaatat atcttccaag aaaactactt aatttcatac taacttcacc aacacttttt
     4561 ccctcactga tttctttaga ataaagaaag agcttttctt ttaagtcagg atagtcatct
     4621 atagttgctt cattgtatac tttactaatt gcatttaaaa gtttttcttc tttagtaaaa
     4681 tccattataa taacctccct accactacct tctaggtgcc catggaccat gattaaccca
     4741 accattaaca ataatttttc caatttctcc tttagcttga ttccaatcta cccaacattt
     4801 ttctttgtta caataaagac catttccata gtaagtagct gcatcaactt ttattcctgt
     4861 accgatgcca gctagacaag ttcctaatat tcctagaata acacaatgtt ttaatacttt
     4921 ctttttcatg attgtttttc cttttcgttt ttttggcgga ttttataatt aacttagcca
     4981 tttggaaata aaaaaacgcc acgtgaattc catgttatat tgaagttacc acaactctca
     5041 atagacaagg atgaattcac atgacgcaaa accatgataa cacagtttca cgcaagggaa
     5101 aacacctaac ctactcagaa cgttgccaaa tcgccatcct gaaaaaggaa aactattcca
     5161 accgtcaggt tgcggatgtc ctgggctgcg tgccgcagac cataaacaac gaaatcaaac
     5221 gcgggaccgt tactcagctt aaccgccaga atcagaacgg gaaggtctac gactattatc
     5281 atgacgtcta cgacgccgat gccgggcagg cggcctacga gaagaaccgc ttgaactgcg
     5341 gcagacggcc cttatgggcc gacacggacg ccttcattga atgggcggac gacatgatgc
     5401 tgttggaaaa atggtccccg gacgtggtgg tgggctttgc cagaaaacac gaattgttcg
     5461 acccggccat tatccctggg accaccaccc tctatcagtg gatcgaccgt ggcatcatga
     5521 aaacgaagaa tatggacctg ctagaaaaac tgtcccgcaa accgaaatcg tcccgtcatg
     5581 gcaaccgccc gaataaacgg gtgctaggga aatccattga agagcgcccg gaggcggttg
     5641 gtacccggaa aaccttcggc cattgggaag tcgacacggt cgtaggaaac aagaccaagt
     5701 cggatgccgt gctgctgacg ttggtggaac ggcagacacg cttcgaggtc attctgaagc
     5761 tggccgggaa ggatgccgaa tcggtcaacc aagcggtccg acagctgaga gaacgggctg
     5821 gtgcgtactt cggccagctg ttcaagacgg ttacctcgga caacggctcg gagttcgcgg
     5881 gactgcacga tgccttgcag gatgtggcaa acatctactt cagccacccc tatgcttcct
     5941 gggagcgcgg gacgagcgaa aaccaacaca agctgattcg ccgtttcatc cccaagggca
     6001 gcccaatcgg caacgtgagc gaaaaccaaa tcttgcggat tcagcgttgg atgaacgatt
     6061 acccgcgaaa gattttgggc tatgcaacgc cgcatgacac gttcgtccag gcatttaggc
     6121 aggaacggtt gacggcttga cacgtgggcc cttgacaacg agcctccctc ggcatggctt
     6181 gtgtctggaa agctggccgg ggccagccag tcgggagact accatgcctc agctcattgt
     6241 caagggccca ctcgattaga aaaattagac ggttggtggt aacgagcaac cgggtcacca
     6301 tgggtggcta acttaaactt gaaatttacg ttttcgtttt tttaaaattg cttgttatat
     6361 taaataatct tagtaatttt attatataac attttattat ataacaatga ttaacgtcat
     6421 aaaaagtagt tttgaattca taatcgttta aataaaatta taagatcagt tatttaaaca
     6481 gctagt
//
LOCUS       DAPCMT010000140         6456 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPCMT010000140 DAPCMT010000000
VERSION     DAPCMT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.143"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..2647
                     /locus_tag="RD743_002395"
     CDS             <1..2647
                     /locus_tag="RD743_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDU0564188.1"
                     /translation="HLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTADSQAIINYLEK
                     QKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKK
                     AENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRR
                     FGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKI
                     LEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLL
                     IFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSV
                     ESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYV
                     DHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLG
                     WKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAW
                     SIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNA
                     TNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQET
                     FSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGI
                     KLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILG
                     YPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPST
                     IWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKR
                     IHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2829..3068
                     /locus_tag="RD743_002396"
     CDS             2829..3068
                     /locus_tag="RD743_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564189.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3084..4457
                     /locus_tag="RD743_002397"
     CDS             3084..4457
                     /locus_tag="RD743_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0564190.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4461..6095
                     /locus_tag="RD743_002398"
     CDS             4461..6095
                     /locus_tag="RD743_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0564191.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6258..>6456
                     /locus_tag="RD743_002399"
     CDS             6258..>6456
                     /locus_tag="RD743_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564192.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2259 a         1055 c         1298 g         1844 t
ORIGIN      
        1 gcatttacaa ctgttaaata cactggggat ttcttatgtt aatttgagaa ctttttctac
       61 attaaacaag aaaaccgttt ctcatatttt ttctactgca gattctcaag caattattaa
      121 ctatttagaa aaacagaaat tgtctgaagg tacagtaaac ataacttata atcctattaa
      181 acaagaggtg attacacaaa aaaagcattc tgtgagagac aatgatattg aaaaaattcg
      241 gtttgtattt attgatattg accctaaaag gaaagaggga agtgcaacag atagcgaaaa
      301 gaaaaaggca gaaaatgtta tggaacaagt agaacgttat ttgaaagaaa agagaataga
      361 atcatttgta aaagttgata gtgggaatgg ctaccatatt tttcttccca taaatgaaca
      421 accaaataat cacgagacga ttcttactat tcagaatttt cttcaattac ttcaccgaag
      481 atttggagta gaagatgaag ttgatattga tacttctgtt tataatcctt caagattatg
      541 caaattagtt ggtacaccag cggtcaaagg aaagtccaca aaagagcgtc cgcatcgttt
      601 atctagtttc ctttctattc ccgataacat agatagaagt aattgtttta aaattcttga
      661 acagatagtt gaagaaaatt ccatgatgtc aacctctaga gtaaaaagcc atccatcttt
      721 tagagaagtg ttacctttta ttcaagcaga ctataaacaa tggttagctc actatcctga
      781 attaacttat accataaaag agggcgatca taaaggagtt accttactta tatttgacga
      841 atgtccatta agaaaacaca cgaataattc taatggttct tgtttgtctg ttacggctga
      901 taataagatt cgatttcatt gtctgcatgc ttcggataaa aataaagata ttcaagactt
      961 tgtaaaaaaa tatccgttgc caaaacctgt aaatccttct gtagaatcat tagaatccaa
     1021 tcaagaatat tattttgacc agtttaaact gaatcaagaa ggagtatttt tggtgaaaga
     1081 aggtcaagaa gaaaaagttg cttgtccgat gtttattaag caaatacgca aaaatatcga
     1141 acaaaaagtc attacacttg ttcttgccta ttatgtcgat cattgttggg atgaaataga
     1201 aatagaaggt ggatgtttac aaactaataa ttttaaacaa ttagcaaaac atggcttaat
     1261 cattaaagct agaagagaag ctgaagtcgt tgacttttta cagattcaac gggagaatgc
     1321 tccaatcttt tttgaacatg aatctcttgg ctggaaaaaa gaaaatgaag caatgatttt
     1381 tcagttacaa gattcttttg gacgtgaaat tccttctgta ttaactaaaa actcatgcta
     1441 ccaattaaca actaatggta cagaagcgga atttgacgca ttggttcata agtatgtttt
     1501 aggaacgaac ttagaacttg cttggtcgat tggtattgct tctattgtgt taggctattt
     1561 aaacgttacg aaggctaata cttttttgag tttactaatt aacctacagg gcaagagcac
     1621 aacgggtaaa accactacgt tacactttat tgcctcttta tttggaaatc caactacggt
     1681 tcttagaaat atgaatgcta ccaataaagc cattataaag ctagcatcaa acaatcaggg
     1741 aggtgttcca ctaatactag atgagttagg agcagcaact caaaataact ttagctcgtt
     1801 gatctatcaa ttagcttcag gagaagaaag gctaagatta gattctaatt gtcagcttat
     1861 gccacaagag actttttctg ttttgaccct tacctcttct gaagagcgtt tggaaaatta
     1921 tttcaatgag aaattaactg gtttgaaagt tcgatatatc gaattttctg atatcaagtt
     1981 tacaaaggat gcgaatcacg cagagaaagt taaaagtctt tcttctaata cttttggttg
     2041 gggtataaag ttatttcttt tgaaactctt tgaacaaggg attacaaaaa ttgaagcaac
     2101 ttttgtcgaa gctcaaaaaa aactatgggc tgttttacct gaacattctt tgaagcaacg
     2161 cattggtaat aatctagctt tgatttatag tggagccctc ctaattcaag atatactggg
     2221 ctatccttta cgattagagc atatacagac ggtgcttatt aattcgtacg aacagttaat
     2281 tgtagatact agtttaggaa gtgagcaaac ggtatttgac cgagtgaaaa cattgttggt
     2341 gatgaatagc cataaatttt taacaaacag tgacaacaaa catacgccca gtacgatttg
     2401 gggaaaagta tcaattcaaa aaaatgggga tattaaggta aatatttttc ctatcccgtt
     2461 tgaggacttg ttaaggcgag aatttcaagt aaaggatcta acctatcttt ttaggcaatt
     2521 gcttgattcg ggtaatatgt gtgcagaaaa agggagaaga acgaaacgta ttcacatgaa
     2581 tcgtcaagtt cttccaacat acgagattct attaccttct tcactcaaat cgtattttcg
     2641 attgtaattt agaataaacc aaagtatgaa agtgaggcac catgttatgg aaatatttct
     2701 tattgattta gcaaaacaac tgattactta tctgtatgat agcgagcaaa cccaaacgga
     2761 agtaaatgaa gcttgatcta tgcttcattt acttcattca atcaaacatg aaagaaagga
     2821 aaattctaat gactaaaaat aaaatgacac taaaagcaga ggttcttctg tatattcaag
     2881 aacatttttc aaatcaagcc ttttttacaa aaccaattta cttggatttc gaaataagag
     2941 gggtatctgc tggatctatc gggggtactc ttcaagcgtt gaagaacgaa ggatatttag
     3001 aaaatcactt tgtgcaacgt tcgttcaata gaagagtcgt gaaaaaatgg tatttagttc
     3061 attcatagag aggggaatca agcatgcgtg tggcttgtta tgatcgaacg tctgtttatg
     3121 aagaagcgaa aattagtaat gacgctcaat atgatgcagt gaaagagtat gttttagaga
     3181 aatatgaaca aattccccaa cgattttcag acaaaggaga atcaggggaa aaagcggagc
     3241 gtccagcttt tcgacagctt agagcttggg tttgtgcgtc agaagaagag ggggtgatct
     3301 atgtttggcg ttatgatcga ttggcacgtc aagccaaaat agccttagaa tttattgagt
     3361 tgtgccaaca acatcaggtt caagtgtatt cgatttctga acctcttcct gcttctttct
     3421 cacacgcgtc aacgaataaa ctatttgtgc aattattgtt cacttttgca gaacaacaac
     3481 gaaacgtcat tattgagaat gttcgtgccg gactttccta taaaaagcaa caacggcaat
     3541 atttgtcttc tgcggtacca tatgggtatc gtttagtgaa tgggaaaatc caacaagaag
     3601 tacacgaagc aaggattgtt cgctatattt ttcaacttta tttgaccaaa aaatatggct
     3661 acaaaaagct ttgtcaacga ttgacgcaac aaaagctttt cttccgcgaa cgtccttttc
     3721 agccctatca tatttatagt atcctaaaaa atccattgta ctatggcgaa atcaaaggag
     3781 gatctttggg gaagtatcta ggaacctttg aaccgattct ctcgaaagca acctttttac
     3841 aagcccaaga gattcgccaa tcaaggtgta cagctaaaaa agatacgtat ctgtacttat
     3901 tacgtcaaaa aattcggtgt cccttctgtg gtcgtcatct gagttcgaag tatcaatgga
     3961 atacaaagaa gacgaagacg cttcattatt atcattgtac cgaccgaagc tgccaaggaa
     4021 tttttcttcg ggcagaaagg gttgagtcag aagtattgga aaagatacag gctttttttc
     4081 aatacgaagg cgtctatcaa gccttgattg cccaattaac gcaacaatta gccgaaaagc
     4141 gaaaagatga aaaacgcaaa gtaatccaac aaaagaaaca agaagcacaa tggattaagg
     4201 catttgaaga gggcaattgt tcactggaag aactcaagca acaactgcaa gctcttcaaa
     4261 agccgatgtc tacttcttcg tttctttcac tccaacagtg tgaagcaaaa gtgaaagaat
     4321 ggcttcaact aaaagacctc tctagtcgac aattattatg tgactatgtt tgtcggattg
     4381 aggtaacaaa ggataaaaca gtttgtggga tttacttccg acaactgaca caaaatattt
     4441 tagaaaggaa cgaatgatct atggaaatga tacaaaccaa acgagtcgca ttgtatattc
     4501 gcgtgagtac acaagaacaa gctttagaag gacatagtat tcaagcacaa gaacaaaatg
     4561 gacgacagtt tgccgaacgg atgggctatg aagtggtaga agtttatgcc gatgaaggaa
     4621 tcagtgggaa atccacaaaa aatcggttcg cctatcaacg aatgatgaac gatgctcgga
     4681 accatcgttt tgatctacta gtgatttgga aattgacccg tctcggacgc aatatgttag
     4741 atgtcttaca aacagtcgag gaattattgc gatatgacgt aggattacat tcgatctcgg
     4801 aacaatttga cgtgaccact tcgacaggta aattgatgtt acaactcctt gggagtttcg
     4861 gtgaatttga acgcaaccaa attgcggaga atgttcaaat gtcaatgcaa agcttagtga
     4921 aagataaaaa gcgttatgca ggaggccgac gtttaggcta tgtcagtggt attgacacga
     4981 atggtcaaaa acaattggtc attgaaccag aagaggcgaa aattgttcgt ctgatctatg
     5041 caaaatattt aggcggagca gggtatcgag cgattgccaa ttttttgaat cgtcaaggat
     5101 atcaaacagt caaaaagaat gctttttcta ccacggcagt caaagatatc ttacgcaata
     5161 aaatttatgc agggtattta gaatatgcgc gctacatcaa ttgggaaacc aaacgacgga
     5221 aggggaagaa tccgagaccg attttagtga aaggaaccca tgaaccgatt atttcggaag
     5281 aagattatca aaaagtacaa gaacgccttg ctttagaagg aaaacaaccc aaatggaacc
     5341 atacaggtga aaatgtattg acaggcttgt tacgttgtcc agaatgtggc gcaccgatgg
     5401 cagcgagtaa tgtcacaaat accctaaagg atggcacaaa gaaacggatt cgatattatt
     5461 cttgttcagt ctttcggaat aaaggagcaa gtgtgtgtca tgccaattcg attcgtgccg
     5521 aacaagcgga aacatttgtc gccgaccgat taaaggagat cgttcagcta ccagaagttc
     5581 ttccccgatt ggtggcagcg ttaaatgaag aaatagtgcg acaaagccaa ccattggaac
     5641 aagaactcgt ggtgttactg gaacgaaagg aagaattgaa gactaaaatt gaaaaatggg
     5701 aagctgcttt ggaagatagt cctgaattgt ttccgatgtt aaaagaccgc ttagacgaat
     5761 tgactgaaaa acgacgacag ttgcacattc gagaaaatga aatcttaggg atctttcaac
     5821 aaggagagcc gattcaagtc aaagatgtcc aacggatatt gactaatttg gatcgttttc
     5881 ttgcccagag tgagaagaag caaatcaaag ccttgtatcg tacctttata gagaaaataa
     5941 cctttgatcc taaccataag gagatgttgg aaattacgat gaaattcacg catgccgtgg
     6001 tacaacaatt gaatgagcaa tatcaaatag ccgtatccaa aacaaaggat acggctattt
     6061 ttgtgttaaa aacacccttt acgttaacga tctagtctag gttatatcaa acaacgttag
     6121 aggggcgcta acgcttctct agcgatttga aatcgttaga gaagtagtta gggaggtcct
     6181 tatcataaat agggatattt attaatgaat atttgaaata taattgtata taagaattaa
     6241 aaagagtggg gatagatatg aaaaagatgg gtaagtgtat tttatgtgaa aaatacactg
     6301 aacttactaa agaacatgta ccacctaaaa aatcagggaa tactggaggt aaaaagacca
     6361 gaactggtaa tttagatgat ttccttaaga gcgattttac aaaaggtgat tttccgaagg
     6421 ggattaaaag gaaacctcaa ggaaatgttt attata
//
LOCUS       DAPCMT010000141         6402 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPCMT010000141 DAPCMT010000000
VERSION     DAPCMT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.144"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(249..926)
                     /gene="nth"
                     /locus_tag="RD743_002400"
     CDS             complement(249..926)
                     /gene="nth"
                     /locus_tag="RD743_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDU0564193.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(933..1631)
                     /locus_tag="RD743_002401"
     CDS             complement(933..1631)
                     /locus_tag="RD743_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDU0564194.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1899..3083
                     /locus_tag="RD743_002402"
     CDS             1899..3083
                     /locus_tag="RD743_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDU0564195.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3089..3532)
                     /locus_tag="RD743_002403"
     CDS             complement(3089..3532)
                     /locus_tag="RD743_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0564196.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3758..4045)
                     /locus_tag="RD743_002404"
     CDS             complement(3758..4045)
                     /locus_tag="RD743_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564197.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4216..5568)
                     /locus_tag="RD743_002405"
     CDS             complement(4216..5568)
                     /locus_tag="RD743_002405"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDU0564198.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5841..6287)
                     /locus_tag="RD743_002406"
     CDS             complement(5841..6287)
                     /locus_tag="RD743_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDU0564199.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1736 a         1270 c         1061 g         2335 t
ORIGIN      
        1 tatttatata atcctttgga atactggact tattttatag gtatattgga aggcattatt
       61 ttctacatcg ggtattttcc caataaaaat aacaactttt atttttatcg caataaattg
      121 taataagtta ccatcctagc tactgagtat gtacatttac tttttcatgc aaaaaagata
      181 aacgaaacag cttttctcca taataacaag ataattcata catttatttg caattaaaga
      241 aaccacgtct acaggcgtgg tttctttttt agcgcctttt cttttagacg catccgattt
      301 ttcccatctt gacatattga tagcaaagga catacctcgc acttgggatt tcttgccgtg
      361 caatgatatc taccaaaaaa aatcagtgta tgatgtgtct ttacccataa ttcttgtggt
      421 actttccgca tcagtgtttc ttctacttcc atgaccgtag catctagttt gcagatcctc
      481 aggcgttttg agacacgttc cacgtgggta tctactgcga ttgctggtat cccgaatgca
      541 tcccccaata cgacatttgc tgtctttctg cctacaccag gaagagacat caattcttct
      601 cttgacgttg gcacttgccc atcgaaacgt tcaatcagct gctgtgcaca cgctttgatg
      661 ttttttgctt tatttcgata caaaccaatc gtttttattt taagaatgat ttcatcgatt
      721 gaagcttctg ctaacgcatc gggtgtcgga aaagaggcaa acaaatctgg cgttgcttta
      781 ttcactgata catctgtcgc ctgggcactt aagatcactg caatcaataa ctcaaatgga
      841 ttattgtgct tgagttctcc atgtgcttcg ggaaacattc catacatcgt ttctagagct
      901 tccattgttt tttgtttgct taacatattc cgctactcct ccaaccaatt tttcagtgag
      961 actttcggca attcttcttg ttgagctgac tgttctactt ctctttgaag aagctgctgt
     1021 tttcttcgtt tttggtcttc ttccacttgt tgttttgtac gtagattttt tctttcccac
     1081 gatagcaaaa tgcggtcaac gtaattaaaa ctataagcct gatttaatac ggcttcccgc
     1141 agtgctagct tcaaaatttc cggctgataa tggtcttctt ctagccattg tccgatccgc
     1201 tggaactcga tcgaagaaag cgggcgtcca aattcttgtt caaataattg atacactgac
     1261 tggatttctt tttcttgtgt tttttcttcg tatttttttt cttgtgtttt taaatactca
     1321 cctaatagat catagatggg gtaaagatta tatcgatctg cttttttacc gttgacaatc
     1381 gtcgtttcta tcttgataaa tccatttgtt acgagacgat ccagtatttg aaaaatctga
     1441 tcttgtttga ttcccatatc catagcaatc atctgcaaat cagggaaagt atctccttca
     1501 agctgtgcca tatgcaactg tagaacaaat aaaaattctt cagattgtag accgatccta
     1561 tgataatttt taagtaaaag attagatatg actgtttgtc cagcgcttaa gtaattttct
     1621 aaattcaaca tgatttctcc tccgctcaat cacagtatac gtaaaaaaaa catgggaagc
     1681 aagaacaggt ttcttttcta tttacttcac tgatcggtca gatttacaat gtcgtctact
     1741 tgatttttgt ttgttcatgt ttttgatgcg tatcttcact tttcggaata aataaattaa
     1801 gtagatttcg ctgttcctta gaatgttgac aaggaaacaa aattactata cactattttt
     1861 gtttccaagt taacattatt gcataaagga gtttttacat gtacagtttc acaaataaac
     1921 attctaaagt ttttggatac gtctctgtct tattcactgg attaggattt actatcgtca
     1981 gtcctgtact tccgttttta acgctgcctt attctcactc catacatcag caagctttct
     2041 atatcacttt gctcatgtct gtttatgctc tagctgcctt cttatctgcc cccattctcg
     2101 gtagcttaag tgatcatttt gggcgtcatc cgatccttat tatcagttta ttaggatcaa
     2161 gtatcggata tttgcttttt ggtttaggaa attcgatatg gatgttgttc cttggtcgta
     2221 tcattgaagg attgacggca ggagaaatct ccaccttata tgcttacttt gccgacatca
     2281 cggaacctaa tgaaagaacc aaagtttttg gatggatggg tgctttagtc ggtataggaa
     2341 caacgctggg tcctattata gggggtctat tagctgagtt ggggaattct gttcctatat
     2401 ttattggagc actctttact ttcttaaatg ccgtttatgg ctacacattt atgctagaaa
     2461 gccttccaat aaaaaaacgt tcagtagatt taagtttctc ccatgtaaga ccttttcacc
     2521 aattacaaca attattcaag atttcatccg ttattccttt attgacagca ggttttgctg
     2581 tttggttggc agctggctct cttcaatcta tcttttctca attttcgatt gacgcctttc
     2641 agtggaaggc tgggttggtt ggtttaagct tctctttgat tggaatcttg gatactgtct
     2701 cgcaattgtt cattatgcct cgattgctca aaaaattttc agaacagcag atcacaagaa
     2761 tagggatgtt cagtgaaatc ctcgcctatc tattcatcac attatctgga attcttctac
     2821 tgccgatttt gtttttattt gggattatct gttatggttt cggtgattct atttttactc
     2881 ctgtattcaa tggccaacta tccaattctg tctcagataa ccagcaaggt cttgtaatgg
     2941 gcggtaccca aagtatacaa tctctatcgc gcgtcatcgg cccgctaatc gcaggacagc
     3001 tttatgctgc agcaccattt cttccgacgg tattcggatt tttgcttatg atttttgcct
     3061 attatcacta caataaaaaa tagtagcttc atttgctact tcgtataaat tctctgtatc
     3121 gaccgataaa acgaaatact gtgtcaaatt cttcttttga catattttca aatacagtag
     3181 catctcgctg tacccatttt tgatggagtt gctcgtgtct attccttatt ttttgtccga
     3241 ttggcgtcaa ttgaaaatag atttcttttt tattctcttc gctttgataa gtgtttatga
     3301 tccctctagc tatcagtttt tttgttagct tactgatggc tcctctagtc atgtaactag
     3361 cattcgctaa ttttgtcacg ttaggatcag ggattttccc gatatgttca atcacttcta
     3421 gttcatttag agtaaatcct gcaaattctt tttccatttc tggtttatta cgatgaataa
     3481 tctcattagc taaatccaga tattcttcta ttagcagttg tcttttatcc atttctacac
     3541 cttctttttc tttcattata ttgttttttt gtttcctatt caacaaagat aaaaatattt
     3601 tgtaggaaaa cccaatctga aaccaagaaa aaaaggggtt gacaaagctt gtcataaccc
     3661 ctttttctga ttaaaagata gtaaagagac agtcatcggt aattgtgatg attttttgtc
     3721 atcacttaag gatgagcggc cttttcaaaa actgtcttta aagatcatca tcagtttcta
     3781 gcacatatgg ctgaccttca tatgtgtact ctgtattcaa tgtgccgtca ttttcatctg
     3841 agtagatgcg tctactgaaa ggaaaacgat gagaaacatt atctttggcg tcatttgctg
     3901 gcatcgtgta atattcctct tgcgtttcat tcaactgaga gagtacttga tctgccaact
     3961 catcaatatt gatcgttaat ttcttcgaag cacttttata atcttctaaa aatgcatctt
     4021 gttggacaaa atattcttta ttcatcttct ttctcacctt tcttttcaaa atcaaataaa
     4081 gctgcttgtt cttttatgta acagctttat ctttgctgct tattgtgaca aaaaattcga
     4141 agaaagacaa ctaattgtac ttttcacaaa aaaagacaga gggtcgtgac aaaattgtgt
     4201 cacaaccctt tttctttatt gtgcagtttc gatttcgcct agcgctgctt gtgcaagtag
     4261 gttcaagtaa ttccatgggc ggtcaaaatg tggttggaag aagaaatctg aaagtgcgag
     4321 atcttctaca gtcattttat tttgaacagc taaagacatg gtatttgctg attgtgtaat
     4381 gtcgtatttg gacatgaact gagcacctac gatacgattc gttccttttt catagactag
     4441 ctccatcatt actttttcag tagttggcat gaactctgga cgataattat cttcaaatac
     4501 agtcgcttgg acttctagat cgttcaaagt tgcgctttct ttggtcacac ctgtcgaacc
     4561 gatcgtccag ccaaataggt aaagtcctga agttccttga gtaccacgat acgccatttt
     4621 atgatctgtc aagttatttc ccacaagcaa accttgacga acggcatttg ttgccaaagg
     4681 aatatagttt tgcgtgccac ttggattgta gttgactact gcagagtctc cagctgcaaa
     4741 aatgtctgga ttacttgtct gcatgtattc attcactttg attgccccgt ttggcagtgt
     4801 atctactttt ccttcgatca gttttgtatt tggacggaag cccacacata aaatgaccat
     4861 atctgtatca aatgtatctg ttggtgtagc tacttgtttc acttttcctt cttcatctgt
     4921 gataaattca gtaacatttt cgcctagcgc aagagtaaca ccgcggtctg tcaattcttt
     4981 ttctaagata tctgtgaatg gtttatctag atatttattc aagatgcgat ctaatccatc
     5041 aatcaatgtc acttgttttc cagattcgac aaaagcttct actaattcaa taccgatata
     5101 tccaccacca acaattacta cacgttttgc ttctttcgct tgtgcaataa tttcatttgc
     5161 ttgattgtag tttttgcata ggagtacatt tttcgcatcg attccactaa tcggtgggat
     5221 aataggccat gaaccagttg tcatcactaa tttatcataa gaaaccgttt ctgtttctcc
     5281 tgttttcaga tcttttgccg ttactgtttt attttctgta tcgacgtctg ttacatcgtg
     5341 ttccatttta actgttgctc ctaatgaaga taattcttca ggatttgagt agaacaagcc
     5401 tgctgggtcc tttactactc cgccgacata aagtgcgata ccgcaagata agaaagaaat
     5461 attgtcattt ctttcatata ctgtcacttc tgcttctgga tgatttttta aaatactttt
     5521 tacagctgca gtacctgcat gagtacagcc aattactact actttcattt atggtccctc
     5581 cgattaacat tttgtccttc gacaaaacta ctataacaga aataaaacgg atacaacagt
     5641 ttaatgcttg tgaattcaca aaggtttgtt tgtagtttca ttccttttca agcataagaa
     5701 cccaatatct acaagattct tattttaata atattcactt ttatatctaa gattgtgaat
     5761 aaaagacaaa aaaataaaaa agctagcgtt ttttttacac agctagcttt ttttaggtat
     5821 aacacttgta gaaactgttt ttacatttgc gccttttttg ctgactcttt acaatccgga
     5881 cagagtccat acaattccaa ccgatgttca ttaatctcaa aatcagtcag tctgcttgca
     5941 gccatttcta tatcttcaag ccctgggtaa tgaaaatcaa caatcttacc gcagttttca
     6001 caaattacat gataatgacg ctttgaactg aaatcaaatc ggctagaagc atcgccgtac
     6061 ttcatttctt gaacaaatcc aatcgaagta aataagcgaa ggttattgta aacagtcgca
     6121 acactcatat ttggaaaccg atgttcaagt gctttataaa tctcatcagc cgttggatgg
     6181 gaatgatgtt caatcaaata ttctaaaacc gcgtaccgtt gaggtgtgat tcgaatgttc
     6241 tcttttttta acttttcgat aacctcttcg actaacttat tttccatgta aacaccccac
     6301 gttgttattc tcgtcttatc atactatttt tacattttaa aatccactta acatgaaaag
     6361 aaaattcaat tttattgtat tttaatgaaa aaagacgtaa aa
//
LOCUS       DAPCMT010000142         6375 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPCMT010000142 DAPCMT010000000
VERSION     DAPCMT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.145"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            37..1059
                     /locus_tag="RD743_002407"
     CDS             37..1059
                     /locus_tag="RD743_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDU0564200.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPREYKVIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEVISLKDICREVAKELKDCERSNGTYDFDRFYQGKGD
                     INSQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1070..3274
                     /locus_tag="RD743_002408"
     CDS             1070..3274
                     /locus_tag="RD743_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDU0564201.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            3295..3444
                     /locus_tag="RD743_002409"
     CDS             3295..3444
                     /locus_tag="RD743_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564202.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            3520..4113
                     /locus_tag="RD743_002410"
     CDS             3520..4113
                     /locus_tag="RD743_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDU0564203.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            4129..5028
                     /locus_tag="RD743_002411"
     CDS             4129..5028
                     /locus_tag="RD743_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDU0564204.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            5031..5516
                     /gene="ssb"
                     /locus_tag="RD743_002412"
     CDS             5031..5516
                     /gene="ssb"
                     /locus_tag="RD743_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDU0564205.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5882..6142
                     /locus_tag="RD743_002413"
     CDS             5882..6142
                     /locus_tag="RD743_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564206.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2314 a         1105 c         1313 g         1643 t
ORIGIN      
        1 cccgattggt tgattgatta tgcagaaaaa gaaggattga ttgccgaact aaagccaaaa
       61 catgaacggc aaaatttctt agttcgagac gatcgtttgg atcatgcggt cgccttttta
      121 tggaaagatc cacagacaaa agagacagta ggggcgagtt atcaaggaac gttcattgat
      181 tatgaacgtt ttggtgagag aggcacatat aagcatatag ataaaaattc gacagcgaat
      241 catggcttta acctaaagat tggtgatcct aaacagttaa aattttttga gagtagtatt
      301 gatttactta gctacgctgc actgaatcgt gatcagttaa acgatacgtg gttagtctct
      361 atggaagggt taaaacacca tgtgatcagt cattattttg gtgaggcagt atctgaattg
      421 agaaagaaac aagcctttcc tcaatccatc gagatttgtg tggataatga ccgtgcagga
      481 catattttct acgaaaaaga acaattaatg ggcgcagttg atccatttac gaatcagaaa
      541 gtacgttgtg aacgaggaat cgctaatgat tggcaagtgc caagagaata caaagtcatt
      601 tatgaagaag tcgcaaaaga agaaaaggtc acaccagaag ctattatggc catacacaag
      661 acagaaaaca atctgcaatt aacgaaccaa ctggtttccg ctcacaaagt caatgcgtct
      721 tttggtcagc agctttctgt taacgattca atagaagtca ttagtttgaa agatatttgt
      781 agagaagtcg caaaagaatt aaaagattgt gaacgctcga atggtacgta tgattttgat
      841 cgtttttatc aaggaaaagg ggatattaat tctcagattc ttttctctta taaagcagaa
      901 caatactaca aaggctataa aaatcatgaa catgaatttg tacctgaagt gaaaaaggat
      961 tggaacgatc aattgaagca tgagatccat caacaggaaa ttagaaagca aaaacgtgcg
     1021 atgttgtttc aacaaggcag acaacaagaa agggagtgag atcgtcacga tgaaaactgt
     1081 cattttagca gaaaaaccgt cacaagcgaa ggcctatgcc gatagctttt ctaaagccac
     1141 ccgaaaagac gggtattttg aaatccaaga tcgattgttt tcgggtgaaa ctgtgatcac
     1201 gtatggtttt ggtcatttag tcgagttaga ttcgcccgat atgtacgatg aaaattggaa
     1261 acaatggtca ttagagcatt tgcctatttt ccctacacat tatcactatc atgttcctaa
     1321 agataagaaa aagcaattca acgtggtgaa acagcaactt caatcggccg acaccattat
     1381 tattgcgaca gatagcgatc gtgaagggga actgatcgct tggacgatca ttcaacaagc
     1441 aggcgctgat caaggaaaaa cctttaaaag actttggatc aactccttag aaaaagaagc
     1501 catttatcag ggattccagc aattacgaga cgcagaagag acctatccca aatttgaaga
     1561 agcacaagca cgccagatcg ctgattggtt gatcggcatg aacggcagcc cactgtatag
     1621 tttactcttg cagcaaaagg gcataccagg aagtttttcc ttaggcagag tccaaacccc
     1681 aacgctttac atgatttatc agctgcagga aaagattaga aacttccaaa aagagcctta
     1741 ttttgaaggg aaagcccaag taaccgctca aaatggcata tttgatgcga agcttgatcc
     1801 caatgaaacc caagcgacgc aagaagcatt tgaagcctat ttaaaagaaa aaggtgttca
     1861 agtaggaaag cagccaggca cgattcttga agtcgaaacc gagaaaaaaa gcgcggcaag
     1921 tccacgtctt ttttctctat ccagccttca gtctaaaatg aatcagctca tgaaagctag
     1981 tgccaaagat actttggaag ccatgcaagg gttgtatgaa gggaaatatc taagctatcc
     2041 acgaacagac accccttata ttacagaagg agagcatgcg tatctgttgg atcatttaga
     2101 tgaatacaag cattttttaa aggctgaatc attccctact cctattcata caccgaatag
     2161 tcggtatgtg aataataaga aagtccagga acattacgcg atcattccta caaaaacagt
     2221 tatgacagaa gcaacgtatg aaaagctgac acctttacaa caagcgattt atgatcaagt
     2281 tttaaagacg acggtggcca tgtttgcgga aaaatatacc tatgaagaaa ctacgatttt
     2341 gacacaagtc cagcagcttc agctaaaggc agtcggaaaa gtaccagtag atctaggttg
     2401 gaaaaagcta tttggcaaag agagcgacag aaaagagaag gaagaagaac cgctacttcc
     2461 taaagtaaca aaaggagaaa cggtgacggt agatctacaa atctttgaaa aagaaaccaa
     2521 gccaccacaa ccttacacgg aaggtacact aatcaccgcc atgaagacag ctggtaaaac
     2581 cgtggatagt gaagaagcac aatccatatt gaaagaagtg gagggaattg gtacagaagc
     2641 gacacgagca aatattattg aaaccttgaa gcaaaaggaa tatatcaagg tagagaaaaa
     2701 taaacttgtg gtgacgaata aaggaatttt gctttgtcag gctgttgaaa aagaaccgct
     2761 gttaacaagt gccgaaatga ccgccaaatg ggaaagctat cttacaaaga ttggtgagca
     2821 aaaagggaca caagctactt ttctagcaaa tatccaaaaa tttgtttccc acttgttgga
     2881 agtcgtccca ggacaaatcc aagccactga ttttggagca acgttacaag aggtgaaagt
     2941 agccagtgaa aagcaagaag cggcgcgtca tttaggatct tgtccaaagt gccaggaaaa
     3001 agaagtcata ctttatcaga aggtggcgac ttgcacgtct gaagcctgtg attttaaatt
     3061 gtgggtaacg attgccaaaa agaaactcac agcgacacaa ctgaaagaga tcattcagaa
     3121 cggccgaaca agtcaaccag tcagaggact aaaaggtcag aaaggttcgt ttgaagcaac
     3181 gatcgtttta aaagaagact ttacaacagg atttgagttt tctgaaaaga agaagaccac
     3241 ctacaaaaga aaagtgagac gtaaaacgag ttagaattga cggagggaaa aacgatgaga
     3301 aatacaacgc ataaagaaca agaaatcgaa aatcaaaaag ccaaaatacg ttcagagcag
     3361 gagaaaatgc gtgccagcaa acgaagacta cagcaattga agaatcaaca acggattcaa
     3421 aaagaacttg aattagatcg ataaatcctc ctgttgttaa tattaaacta acatgttata
     3481 gttatgttag tttaatatta acattcgaga gggaaagcga tgaaagataa agtcaagtgc
     3541 atttttgaga aatacaatgg taatctatcc ttgaaggatg ctagaaatga agggttatca
     3601 cctatgacgt tagatcgttt agccgaaaag gacaaaatag aaaaaatagc atcaggtttt
     3661 tataccttaa aagatagttt tgtggatgaa ttatatctcg ctcaaagtat ctatactaga
     3721 ggaatcttct ctcacgagac agcgcttgat ctttttcaag taggaacata tatccctaaa
     3781 aaaattaata tgatgtttcc taaaggatac aatgtttcga aagagaattt agatcagtat
     3841 gccattcaac cgcattacac gaataaagaa aactttgaac tgggaatcac agaggcagaa
     3901 agcttttacg gcaataaaat tttcgtctat gatttagagc gaacgttatg tgatatgtgg
     3961 aatcccagat ataaagcaag cgtagaagta aaacaagaag cattaaaaga atatatgaca
     4021 agtaccagca gaaatacaaa taagctaaga agatacatga atattttaaa atcaccaaaa
     4081 gaaatgactt tgtatatgtt gccactttat tgagaggagt aaaggtgaat gacaccgaca
     4141 caatttaaag cgaaaacaag aaatgttgca gctgagaaac atttaaaccc acaaattgtt
     4201 caaagatatt atatgatgga aaaattatta gaatgtattg ttgaatcgcc ttatcatgat
     4261 gattttgtat taaaaggggg atttctgatc ggatcaaaat acgggttaga aaaccgttca
     4321 accgttgata ttgatacgac ttatcgaaat tcgaaactaa caaaagaaaa attagaaact
     4381 gttttttctg aattaacagc tgctcctaca aaagatggca ttcggtttga attaaagggc
     4441 ttaacagaga cgagggaagc cgattattac ccaggttttc aagcaaagtt tattgctttt
     4501 cttgaaaatg cacgtgtacc ttttaaatta gatattactt caggtgattc tattattcca
     4561 gcagcattga tatatgaaca taaattgatg tttgaagata aaacaattca tattccagct
     4621 tatcctacgg aacaaatttt agctgaaaaa ttacatgcca cgtttagttt cggaatagat
     4681 aattcacgta tgaaagattt ctatgatctg tatatgatcc ctaaactaga acgtatcgat
     4741 gagataaatt tgtataagag tgttcaagga acatttaagg aaagaaataa tcaatcagtg
     4801 tttaagaatt tgtataatca ggaaatgcct caattgatga agaatgaggg attgagggag
     4861 aaatggaaaa gatatcagca agaaaactat tttgccaaag atatttcttt cgaatctacc
     4921 ttaacttctg ttgattcgct gatgcaatca attgtcgagc aagaggaagt agaacgaaaa
     4981 caaaatttta agagatcaca acaaatgaat caagaaatgg agagataaag atgatcaata
     5041 atgtaacttt agtaggtcga ttaaccaaag atcccgattt acgttacacc gcaagtggca
     5101 ctgcagtgac cacttttacc ttagccgtta accgtaattt tactaatcaa agtggcaatc
     5161 gagaagctga ctttatcaat tgtgtgattt ggcgcaaacc agctgaaacg atagcgacat
     5221 tagctaaaaa aggcagcttg atcggcgtcg tcggacggat tcaaacgcgg acttatgaca
     5281 accagcaagg acagcgtgtc tatgtgactg aagttgtggc cgataatttc caactattag
     5341 aaagtaaagc agcgacagaa agccgtatgc acgctgatca atcgtccagc agtccaagcc
     5401 ctaccacctt tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac
     5461 agaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttctaagtag
     5521 cattttctat tggttgcatt tgctacggtt tatgttataa tttagacaac aaaaagaatt
     5581 ttcaatctat aaccaaaaac cactagctgg cgggctagtg gctttctttt acgtttctat
     5641 tataagaatt aacgggtagg cttactgtct ttcttggcaa atttacaatc aagccatttg
     5701 cagatatagt gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa aagaattttc
     5761 aaacctataa cctcctcccc ttgccagatt aggccggggt agcagacagc aagactatta
     5821 tagcataatt gaaatatcca aatgattgga tttttgctat aattttagcg aggtgattgt
     5881 gatgaacaaa tatgttggtt tgcttgataa aatccgagta ataaaaacgc aaccgttatt
     5941 ggttcgcttt actttacaaa cgattcatga atcgattaat tgtgtagttg cagatattga
     6001 aattatcgat aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca
     6061 cttcaataaa cgaaatcagc tagttattgc tagcatgtat gttagaaatc ccgatcattt
     6121 tacgcgatcg atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc
     6181 tctttttgtt ttacatagaa acaggaaaag acctttttag gaagacacaa gtgccttctc
     6241 aattgaaaaa cctttctctc actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa
     6301 cgctgcacgt tcactaagat atggtgtctt gcaaagcaag acaaaataga caaaacttcc
     6361 tctcaccaaa aggtt
//
LOCUS       DAPCMT010000143         6366 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPCMT010000143 DAPCMT010000000
VERSION     DAPCMT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.146"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(145..333)
                     /locus_tag="RD743_002414"
     CDS             complement(145..333)
                     /locus_tag="RD743_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDU0564207.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            603..965
                     /locus_tag="RD743_002415"
     CDS             603..965
                     /locus_tag="RD743_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDU0564208.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1017..2699
                     /locus_tag="RD743_002416"
     CDS             1017..2699
                     /locus_tag="RD743_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDU0564209.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2816..4852
                     /gene="recG"
                     /locus_tag="RD743_002417"
     CDS             2816..4852
                     /gene="recG"
                     /locus_tag="RD743_002417"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDU0564210.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4901..5902
                     /gene="plsX"
                     /locus_tag="RD743_002418"
     CDS             4901..5902
                     /gene="plsX"
                     /locus_tag="RD743_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDU0564211.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6024..6266
                     /gene="acpP"
                     /locus_tag="RD743_002419"
     CDS             6024..6266
                     /gene="acpP"
                     /locus_tag="RD743_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDU0564212.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2130 a         1103 c         1437 g         1696 t
ORIGIN      
        1 taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa cgcaaaaaaa
       61 gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac cgttcactat
      121 ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt tcaaagcacg
      181 agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt tttgtaagtt
      241 aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc cactttttgt
      301 cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg ccttgatttg
      361 gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg ggaatttgca
      421 agtacatttt catagataca attgacaatt gagaagaata attgtaaatt aaacaaggtt
      481 atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta tttcaagttg
      541 cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa ggaggctcat
      601 tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat gatgtaattg
      661 caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa
      721 atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca agaggagtag
      781 ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc agctatggta
      841 cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat
      901 tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt gtactgccag
      961 actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt atctaggtga
     1021 gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc
     1081 tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca gatggcgata
     1141 cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta gattcaacct
     1201 cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg
     1261 gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa attccagatg
     1321 ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt gaaactgcgt
     1381 ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga gaagcagcga
     1441 aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg acagctgttg
     1501 taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc ctaaaagaag
     1561 tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc tttttaagtg
     1621 cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca gaaatggatg
     1681 atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca gaagatatcc
     1741 attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca gtcgatagta
     1801 cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct ttacttgtgg
     1861 tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga gaagtcttga
     1921 attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg cgcttgcagc
     1981 atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac
     2041 gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa
     2101 gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt acggaagaca
     2161 ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca aacaataaga
     2221 acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta gcagttgttc
     2281 cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat caagcttctt
     2341 tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca
     2401 ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga gactttttgg
     2461 gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca gcgttaagta
     2521 cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca ggtgatgaag
     2581 gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat cctgatttgg
     2641 aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct gctgaataat
     2701 ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt cggttagtga
     2761 tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa aatttatgcg
     2821 ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag cagctagtct
     2881 tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt ttcgctacga
     2941 agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg
     3001 aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc gattgcaatt
     3061 tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac cttatctaaa
     3121 agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa
     3181 agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt cgccgatata
     3241 tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa aagggtttca
     3301 agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga aatatcgact
     3361 gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg aagaaaacca
     3421 tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc gtcttcaagg
     3481 attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg atgtccaaaa
     3541 attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa aacgtgtaac
     3601 gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga
     3661 tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg ttacagcagg
     3721 tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata tggaaagctt
     3781 gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt caacgaaagc
     3841 aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg
     3901 cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag ttattacgga
     3961 tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc
     4021 agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta cagcatatgg
     4081 tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga tcgaaacacg
     4141 ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag aactggcaag
     4201 aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat tagatgtaaa
     4261 aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat atgaagttgg
     4321 cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag ccttcaaaga
     4381 caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa
     4441 cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc tgcaccaact
     4501 tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg ctaatcctaa
     4561 aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg gttttgtact
     4621 aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg
     4681 tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg aggtagccag
     4741 agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg agtatcagcc
     4801 tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt agcagactgt
     4861 gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc
     4921 aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga
     4981 ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga aatacttaac
     5041 aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg atgatgagcc
     5101 agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac aagcagtgaa
     5161 aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac tagcagcagg
     5221 gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc
     5281 agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta acgcagaaaa
     5341 caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac gtaaagtccg
     5401 aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa caaaaggtag
     5461 tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga attttattgg
     5521 aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca cagatggatt
     5581 tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga tgaatctatt
     5641 aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga
     5701 cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg ccgttttgtt
     5761 tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg ctgttcgcta
     5821 tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat tagtcgaaca
     5881 atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc attgaaaaaa
     5941 aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg
     6001 tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa
     6061 ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg aacatcaaag
     6121 atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt gaagatgaat
     6181 tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt ggcgcagtag
     6241 attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat aaaaagctgc
     6301 atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca tttttctgat
     6361 aattct
//
LOCUS       DAPCMT010000144         6310 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPCMT010000144 DAPCMT010000000
VERSION     DAPCMT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.147"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..1313
                     /locus_tag="RD743_002420"
     CDS             <1..1313
                     /locus_tag="RD743_002420"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDU0564213.1"
                     /translation="YLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQ
                     SKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFY
                     TPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNT
                     TTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTT
                     FLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQK
                     LLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLS
                     EENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGN
                     DIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1303..2520
                     /locus_tag="RD743_002421"
     CDS             1303..2520
                     /locus_tag="RD743_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDU0564214.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2533..5682
                     /locus_tag="RD743_002422"
     CDS             2533..5682
                     /locus_tag="RD743_002422"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HDU0564215.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2200 a         1049 c         1296 g         1765 t
ORIGIN      
        1 agtatctttt caacgtctta gcggatcaag caaaacaagc aacgttccaa ttgaacgatc
       61 taaacaaagc ctttgtgcaa ttggcatcta cctacaatca atttaatggg ttatttgatg
      121 atgtggatct ccaatcaaaa aaactaggaa cagatgagca acaacgaaat gtgacgatta
      181 ccgaagtcat caagaaattg aatgatgtag atgttttaga acacgatggg gacgtgatcg
      241 gggatgcgta tgagtttctg atcagtcaat ttgcttcaga agcagggaag aaagccggag
      301 aattttacac acctcatatg gtttctgata tgatggcgca aatcgtaacg ctcgatcaaa
      361 aggaacgacg attctttagt gtttttgatc caacgatggg ttcaggttca ttgatgttaa
      421 acgtacgtaa ctacttaaca cacccagata acgttaaata tcatgggcaa gaactaaata
      481 caacgacata caatctagca aaaatgaact tgattttaca tggtgtcgac gccgaagaaa
      541 tgaatctccg caatggggat acgttgaata aggactggcc aacagatgaa ccttacacat
      601 ttgatgcagt agtaatgaac ccaccttatt cggctaattg gtcagcagat acgactttct
      661 tagatgattc tcggtttaat cgttatggga aattagcgcc aaaatccaaa gcagattttg
      721 cgtttcttct tcatgggttc tatcatttga aggaaacagg taccatggcc atcgtcttac
      781 cacacggagt tttattccgt ggcgctgcag aaggggtcat ccgtcaaaaa ttactagaag
      841 atggcagtat ttatgctgtg atcggtatgc ctgcgaactt gttctttggg acatcgattc
      901 caacgacggt gattgtgttg aagaagaatc gtcaaactcg agatgtatta ttcattgatg
      961 ctagtcgtga atttgttaaa gggaaaaatc aaaataaact atcggaagaa aacattcaaa
     1021 aaatcctcga aacctacgct gaaagaaaag atgtcgagaa atacgctcat ttggcaacct
     1081 ttgatgaaat caaagaaaat gactataact taaatatccc acgctatgta gatactttcg
     1141 aagaagaaga accaattgat atggttcatg tcggaaatga catcaagaag atacgacaag
     1201 aacagcaagt gctagaaaaa aaacttttag aagctatctc ttccctgcaa acaacacctg
     1261 aaaatgaagc gtggttacaa ggtgcgctgg aggtgttcaa acatgagcaa tgatacacaa
     1321 ccagaaatcc gtttcccagg atttacagat gattgggaac agaggaagtt gggggacttc
     1381 gttgtagatt atgttgaaaa gacaagtgta caaaatcaat ttcctatgct aacttcatct
     1441 caacaaaaag ggatagtttt gcaagaagac tattttgcaa atcggcaagt aactacagaa
     1501 aataatattg gttactttgt actacctaga ggatatttca cgttccgtag tcgaagtgat
     1561 aatgatgtgt ttgtgtttaa ccgtaatgac attattgata gagggattat aagctatttt
     1621 tatcctgtat ttactttaaa atctgctgat tccgacttct tcttaagacg tattaacaat
     1681 ggaatccaac gacaattatc tattcaagca gagggaacag gtcaacatgt tctgtctcta
     1741 aaaaagttta aaaatatagt agcaatgttt cctagtgagg aagaacaaca aaaaatcggc
     1801 accttcttca aacaacttga cgacacgatc gctcttcatc agcgtaagtt agatttgctg
     1861 aaggaaacga aaaaaggatt cttacaaaaa atgttccctg aaaacggagc aaaagtgcca
     1921 gaaatccggt ttcctggttt tacggaagat tgggaagagc gtaagttggg agaattaggt
     1981 aaaactcaat cgggaattgg atttcctgat gccgagcaag gtggttctga aggtactcca
     2041 ttttttaaag tttccgacat gaataacatt ggtaatgagt atgaaatgag aaatgcaaat
     2101 cattatgtat ctaatgagca aattgagcgt aaaaaatgga agccaataaa agatgtgcca
     2161 gcgattatat ttgctaaagt tggcgcagca ataatgctta atagaaaacg tttggtcaca
     2221 agccctttct taatagataa caataccatg gcttatttat ttaataatac ttgggatatc
     2281 tattttggta aaatattgtt tgaaactata aatttgccaa gatactccca agtaggggca
     2341 ttgccttctt ataacagttc tgatattgag aatatcagtg ttaaggttcc tgtaaaggat
     2401 gaacaacaaa aaatcggtac tttcttcaaa cagctagatg atacgatcgc tcttcatcaa
     2461 cgtaagttag atttactaaa agaaacgaaa aaaagcttct tacaaaagat gtttgtctag
     2521 gagggaagaa gaatgaaaat tagtcaccgt gaggaagcag aagtcgaaga acagttgatt
     2581 cgtgtacttg gagaagggca taatcagtgg acgtatcgtc cggatctaaa atcagaagaa
     2641 gacctttggg tcaatttacg ccaaaaaatt atctcaaata atcaagcaga actgaatgat
     2701 tttcctttga ccgataaaga atttgaaacg atcaaaacgg aattactgct gcgaacgaaa
     2761 accccatttg atgctgcgaa atggttaaaa ggtgaaaatg ggatggctcg gatcacgatt
     2821 gaacgggaag attcgcaatt gggttctgtc tcacttatcc tttattccaa tcaagacatt
     2881 ggtggaggaa tctctactta tgaagtggtc catcaaattg caaaacgtgg aagtaacata
     2941 gaagcacggg atcgacgttt tgacgtcacc ttattaatca atggattacc gattgtccaa
     3001 atcgagctga aacaagtaac tgcaaaagat ggtgtttatc aggcatttaa ccaaatcaaa
     3061 aaatatgcag aagaaggtat gtttcggaac aatatctttt cgaccttaca gttgtttgtg
     3121 gtttccaatg aacaaaccac tcggtatttt gcaaatgcct taccgaaaga cttgcaccct
     3181 aagtttttat ttagttggcg aacgaaagac aatgaaaaag tagaaaactt atatgagttt
     3241 tgtaaacaag tgttaaatat cccggatgct catcgtttga ttgcggatta tacaatcgtc
     3301 agtgaagatc aagacaataa aaccctcatg gtgttacatc cttatcaagt acatgcgatt
     3361 caagccttgt ttattgccgc aaataaacat caatcagggt atgtgtggca tgcgactggt
     3421 tcaggaaaga cgttgactag ttttgtatct actaagcttt tagcacgaaa atcggggatc
     3481 gatcgaacga tcatgttagt ggaccgcaaa gatttagata atcaaaccac cacagagttt
     3541 acaaaatttg cttcggaatt taatactggg atttcttccg ggaacgccaa agcgaatagc
     3601 ttgatcgttg gaacgggaag tgccaaagaa ttaagtgaaa ccctgttggc agatgccaat
     3661 gcaaatgttg taatcattac aacccgtcaa aaactagatg cggcgttgaa atatgcaaag
     3721 aaacaagaag agaaaaaagg aaccaatcgc ttccaaaaat taatgggcca acatatcgtg
     3781 tttgtagtag atgagtgtca tcgagcgtta agtgctgaaa atatggaaga aatcaaaaaa
     3841 atgtttccga aatccacttg gtttggcttt acaggtacac cgatttttcc tgagaatcgg
     3901 aaacaagcca aagggcaatt ggcacgtaca acccatgatc agtatggaga ggtcttgcat
     3961 acctatacca ttaagaatgc gctagaagat ggctctgtct taggatttca agtagagcat
     4021 gaaaacaccg tggagccgac ttcactagag aataaaatct atcggaaatt aaaagaagta
     4081 gaaacatatg cagagtattc atcagaacag atcaatcgta tgattgatca aatggaacct
     4141 gtcaaaaaag aaagttattt ggacccagct gtttttgaag cagatgaaca tatccaaaaa
     4201 gtcattcata aaatgttccg tccagataat gcctatacaa aatttgattt tcgaaatggc
     4261 aggccaacga aatctgcaat cttaaccacg agttccattg acatggccaa aaagtattat
     4321 cgggccatca aagagatgac gaaagagcct gattggttaa caaaagagtt tcccaatcag
     4381 ctcattcgtg aaggacgaac gatggaggat cctgattttc cacgaatcgc tattacctat
     4441 tcattagaag aaaatagccg agattcaagt gcccaacaag aagaaatgca aaaaattatt
     4501 gaggaatata atcagtatta tgatacttcc tggtcattag cagacattga gcgttataac
     4561 ggagacatca ataatcgttt ggcacgaaaa agagcggaat tcaaacaatt tgggaaacaa
     4621 atcgatttag cgattgtagt ggatcgtcta ttaacaggtt ttgacgcccc tacgattcaa
     4681 accctatttg tggatcgtaa tctagagtat gcaggattga tccaagcttt ttcacgtacg
     4741 aatcgtacgt atcctgataa gacaaaagga ttgattgtga cgtttcgtaa gccagcgaca
     4801 atggagaaga atgtcgagga tgcgacaaaa ctgtattcaa aagcaaaaga agaatcaggt
     4861 ttgatttatc caagttatca ggaatccaaa aaaagattca aacaagcata tggtaagtta
     4921 aaagaggttt catccatgga atccattgat gaacatacgc cacttgaaat ccgtatcgag
     4981 tacgtcaaag catttcaaga attgaatcgt gcttatgaat cgttagtgac ttatgacgat
     5041 tacaatgatg acatggtgaa atctccaact ttgaacaatc aaattcaact tttggaagaa
     5101 caaattggcg tgtacgagac cgtgaagggt tcattaatag aggaagaacc agaaaaagaa
     5161 ggagaagatt tttcaacgat cgaattttat agtgaaaata ccactaagtt gtatgatatt
     5221 gattcaactt atattgatca attactagga acttatgctg cgaatagttc agatgcacga
     5281 gaagaaattg aaaaagcttt ggctaaattg aataaaacag aaggagtcaa agaagtttac
     5341 cgacaaattt taaatgcatt tgatgtgggg actttagata gtaatgaaga tatctttgtc
     5401 attaaacgtc gctattttac acaagcgagt gatgatttga ttcatgtctt ttccaatgag
     5461 tggtttgttt ctgagaaaga attacatgct tctaatctgc aatatctacc aggtgaagat
     5521 ccaattccta atatgaaagc aattatcaac agtaaggatt acgaaggata taaagccaaa
     5581 catccagaag cgaagccatt taaataccca caagaaatga agcgagcatg gcgtaaaatg
     5641 ttagatgatg agttgattcc acttgagaat gaattgaggt aaacgagaag agttaagaaa
     5701 attgtacgat tcaaagatgg tagtcaagtg gaagtgtaga aaatagggat agatgtcttt
     5761 tatttgttca cattttataa cgaagtattt aagacagcta ttgaataatg tttcctccac
     5821 ctttttcatt catttacccc accccgttgt tatatgaccg tgttttgaga ggaaccaata
     5881 aaaagcatag gctaactagg ggttcctttt tcttttgttt ttttggaatc cgcttaagtg
     5941 aaatgtatct gaaatgaaac ggagataaag tgattttttg ctataatgac aaggttttag
     6001 ctgtttcaga taccatgtaa cccaaaatag tttattgaga aaataggaga gtatttgata
     6061 ctaactagta ggaaaaaatg agataaaaat taaatggaat catttcaggt aatctcaaat
     6121 aaaaaaatat acagtcctgt ggcactttac ctatgggact gtatattttt tactctcagc
     6181 gggcttgttt tgactccgtg tctgactccg tcgtattgat tttaaatgat attcgatgac
     6241 catttaacag gtcatttttg aaatttttgt ctctatatga ctcagtttga taattattga
     6301 tagatcaatt
//
LOCUS       DAPCMT010000145         6287 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPCMT010000145 DAPCMT010000000
VERSION     DAPCMT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.148"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..177
                     /locus_tag="RD743_002423"
     CDS             <1..177
                     /locus_tag="RD743_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564216.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="RD743_002424"
     CDS             190..690
                     /locus_tag="RD743_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDU0564217.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="RD743_002425"
     CDS             787..2634
                     /locus_tag="RD743_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDU0564218.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="RD743_002426"
     CDS             2637..3533
                     /locus_tag="RD743_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0564219.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="RD743_002427"
     CDS             3583..3972
                     /locus_tag="RD743_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0564220.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="RD743_002428"
     CDS             3959..>6287
                     /locus_tag="RD743_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0564221.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAPCMT010000146         5938 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPCMT010000146 DAPCMT010000000
VERSION     DAPCMT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.149"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            361..642
                     /locus_tag="RD743_002429"
     CDS             361..642
                     /locus_tag="RD743_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564222.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            644..1060
                     /locus_tag="RD743_002430"
     CDS             644..1060
                     /locus_tag="RD743_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564223.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1682..2836
                     /locus_tag="RD743_002431"
     CDS             1682..2836
                     /locus_tag="RD743_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDU0564224.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2830..3252
                     /locus_tag="RD743_002432"
     CDS             2830..3252
                     /locus_tag="RD743_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564225.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4422..5390
                     /locus_tag="RD743_002433"
     CDS             4422..5390
                     /locus_tag="RD743_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDU0564226.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            5407..5931
                     /locus_tag="RD743_002434"
     CDS             5407..5931
                     /locus_tag="RD743_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDU0564227.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
BASE COUNT         2252 a         1015 c          963 g         1708 t
ORIGIN      
        1 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
       61 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
      121 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
      181 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
      241 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
      301 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
      361 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
      421 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
      481 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
      541 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
      601 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
      661 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
      721 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
      781 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
      841 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
      901 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
      961 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     1021 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     1081 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     1141 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttgaggcg
     1201 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     1261 ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat cctattcatc
     1321 aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat atcaaatcct
     1381 ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa ctaaaaaaga
     1441 gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt aaaaatcaca
     1501 aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtgtaca ttatgttaac
     1561 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     1621 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     1681 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     1741 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     1801 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     1861 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
     1921 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
     1981 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
     2041 agaatattca ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag
     2101 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
     2161 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
     2221 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
     2281 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
     2341 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
     2401 agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat
     2461 gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga
     2521 tcaaataata aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact
     2581 aaacctttca gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc
     2641 tggttatgga aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca
     2701 aggtggttat caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag
     2761 tgcaaatgca ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac
     2821 cattacaaat tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc
     2881 ttaactagtt gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga
     2941 acttggcaaa ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa
     3001 aatatagaac aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat
     3061 aaaaatttaa atataaatga acaagttttc atttacaaag caacaaataa tttactaaca
     3121 ctgcgctcta ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc
     3181 gtatttattt tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt
     3241 aaagctgatt gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa
     3301 caagcgaaac tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt
     3361 gggttttgag ggggattcaa gggggagctt gcgccccttt gaaaagcggt tatttgcacc
     3421 aaatacgtag gtatttggct agcaaatata gagcttgcca aacactttag tgagttttgt
     3481 agaaagtacc tttaaaggta ctttcacatt acgaacgata cgtccgtagg acgtactgaa
     3541 agggtgggat ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata
     3601 tatgctaata atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag
     3661 ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca
     3721 cacgagcaaa tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag
     3781 tatataaata tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag
     3841 acaactttag ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat
     3901 aagggattta tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa
     3961 gagttcctta tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag
     4021 agcagagcga tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa
     4081 tgcgcgaatc tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct
     4141 cttttataat ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac
     4201 tctcccaagg gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg
     4261 tctgtttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga
     4321 attagtacat cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa
     4381 cgtgtatctc ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa
     4441 agtatcatca tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt
     4501 tgttcttttc tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat
     4561 tcgagcaatt aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg
     4621 atttagaaac aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg
     4681 gtttgcaacg tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata
     4741 ttccttttgc tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag
     4801 aatgggtgcg ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa
     4861 tgtttcgatt actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact
     4921 ttcatgaatt acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt
     4981 tcaatccaat acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat
     5041 acggaaaagg acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa
     5101 aaaaatcaga cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag
     5161 aactagctaa ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca
     5221 aagaacaaaa gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa
     5281 atacccaaaa taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag
     5341 aactaaaaaa agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta
     5401 gattttatga cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca
     5461 gcaattaaca aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac
     5521 aagtttgtga ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac
     5581 gaaaactcaa atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc
     5641 aaccaagttc ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat
     5701 atgcaactac aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa
     5761 caacaactca ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg
     5821 acttatacgg aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg
     5881 atagaaaaac aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaaccagc
//
LOCUS       DAPCMT010000147         5890 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPCMT010000147 DAPCMT010000000
VERSION     DAPCMT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.150"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..985)
                     /locus_tag="RD743_002435"
     CDS             complement(<1..985)
                     /locus_tag="RD743_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HDU0564228.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="RD743_002436"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="RD743_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDU0564229.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="RD743_002437"
     CDS             1810..2964
                     /locus_tag="RD743_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDU0564230.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="RD743_002438"
     CDS             3179..4147
                     /locus_tag="RD743_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDU0564231.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="RD743_002439"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="RD743_002439"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDU0564232.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..5785
                     /locus_tag="RD743_002440"
     CDS             5177..5785
                     /locus_tag="RD743_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDU0564233.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1776 a         1148 c         1331 g         1635 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt
     5581 accaatgggg agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat
     5641 atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt
     5701 tgaagcatat agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag
     5761 ctattttgat ttccccgtta aataaacttt taaccgttgc acggacaaac tatataagct
     5821 aactctttcg gcaggaaacc cgacgtatgt aactggttct tagggaattt atatatagta
     5881 gatagtattg
//
LOCUS       DAPCMT010000148         5887 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPCMT010000148 DAPCMT010000000
VERSION     DAPCMT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.151"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..495
                     /locus_tag="RD743_002441"
     CDS             <1..495
                     /locus_tag="RD743_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDU0564234.1"
                     /translation="SEVLPAIRKHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRL
                     IAEQKIAEYEPKISYLDSILSSTDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQ
                     WLLCKKHMNQGYTKSHTTEIPKADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDL
                     EEIG"
     gene            510..713
                     /locus_tag="RD743_002442"
     CDS             510..713
                     /locus_tag="RD743_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564235.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            729..1049
                     /locus_tag="RD743_002443"
     CDS             729..1049
                     /locus_tag="RD743_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDU0564236.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            1054..1224
                     /locus_tag="RD743_002444"
     CDS             1054..1224
                     /locus_tag="RD743_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564237.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPVGRD"
     gene            complement(1231..2013)
                     /locus_tag="RD743_002445"
     CDS             complement(1231..2013)
                     /locus_tag="RD743_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564238.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITEKFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            2064..2771
                     /locus_tag="RD743_002446"
     CDS             2064..2771
                     /locus_tag="RD743_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDU0564239.1"
                     /translation="MQELVILKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQK
                     YADLFWEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEK
                     AIRKNTAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVL
                     GGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRM
                     EIDQLNRQQRLFGDENE"
     gene            2949..3290
                     /locus_tag="RD743_002447"
     CDS             2949..3290
                     /locus_tag="RD743_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564240.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            3283..3954
                     /locus_tag="RD743_002448"
     CDS             3283..3954
                     /locus_tag="RD743_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDU0564241.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3960..4646
                     /locus_tag="RD743_002449"
     CDS             3960..4646
                     /locus_tag="RD743_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564242.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            4653..5504
                     /locus_tag="RD743_002450"
     CDS             4653..5504
                     /locus_tag="RD743_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDU0564243.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            5516..5785
                     /locus_tag="RD743_002451"
     CDS             5516..5785
                     /locus_tag="RD743_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564244.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
BASE COUNT         2215 a          914 c         1165 g         1593 t
ORIGIN      
        1 agtgaagtgt tgccagcaat tagaaaacat ggaggttatc taactccaga aaaagtagaa
       61 gaagctttgc ttaatccaga tacaatcatt caattagcaa ctcaactaaa agaagaaaga
      121 actggaagat taatcgcaga acaaaagatt gcagagtacg aacctaaaat ctcctattta
      181 gatagcatat tatcttctac agattcagta acaattagtc agattgcagc agattatggg
      241 atgtctccac aacagatgaa taaattactt cataaactag gtattcagaa aaaagtcggt
      301 aaccaatggt tattgtgcaa aaaacacatg aaccaaggat acacaaaatc tcatacaact
      361 gagatcccga aagccgatgg tggcactaaa attgtaatga ataccaaatg gacacagaaa
      421 gggcgtctat ttatctacga attactaaaa aaagaaggat attaccctca aatggattta
      481 gaggaaattg gttagaaagg agttttagta tgactgacat tgcagaaatc actcaacgag
      541 atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt cttaacttac accatgctcg
      601 ctgaaagatt cggcattagc aaaagctact tatctttaat tttaaacggt aaaaagactt
      661 ctgcagaagc aaatagaatc atagattcga ttattactat gtacgaattg taaagggagg
      721 aacagctaat gcaatatcta gaagcgaaaa tcccagttcc agaaggctat gtaattatct
      781 cccaagtgga ttatgaggag ttaaaaaaag ctgatgatac tggtagatgg atgacgttgc
      841 cagaagtgct agaacggatt aacagaaaat atgattggtt tacttctaga gttttaaaga
      901 acccaagata tagaaatatt atcgatatcg aaaaaaacaa aaatggattt gtctattatc
      961 cagttgaagg aagagacaca tatctatttt taagaagtaa aacacttgaa tttttagaaa
     1021 caaatttttc ggaaatctta aggaggtaag cggatgggta aatttaatag agcactagta
     1081 ttcagcgcac cactaattat ctacgcttta ggtctttggg gaagcagaca agcattaatc
     1141 ggcacaattg tatatatggt ctggatcttt atggggcttg atgaagctga atatagagcg
     1201 aaaaagccag tcgggaggga ctgacttaac ctaattaatt aaaaattgta acaaataacg
     1261 caaaaataaa gctaatgaaa ttgtattgtt aggatctact ccaatagtta tatttacaat
     1321 ttcggttact actagtccta tcaaagctgc cccagtttgc tctaaatacc acattttatc
     1381 tttgtagaaa gcaggtgaag cttgttgttt ttcagatggt tcattagtta tatctttgtt
     1441 cttggctgta tttatttctt caaaaagctt tatagaatca tcaaggactt tcctttgctc
     1501 attaaggata tcaaaaaact tagcctcaaa ctcatcagat ggattagaag tgtctttaaa
     1561 aagtagattt tttgtactaa cttccggttc ggaagaaaat gctttttcta attcttgggt
     1621 agctacccct attctgaagt cgtacttaga catgtcaacg gcaaattttt ctgtgatttt
     1681 agagatatca gaaaaataag gttttggcat agtagctgcc atttttttag caatattaaa
     1741 acttggagat tctaattgtt tctgtattgc gaacaaagaa ggaggaatca tattagcaat
     1801 agatttgtta cgtttctcga caatttgaat gagagaatca ttgagtattg cttgcgaagc
     1861 tataaaagta tcttgaattg caattaattt tttagcttgt tctatcgcag gctgcatagc
     1921 gttaacgaca tctagagaag gagcaatggt ttcaccgatt aattttgaag aagctatcaa
     1981 tttttgataa taaggagtat ctgaaatatc caattatata caccaccttt ttacctaaat
     2041 tataccaaaa gggagagaaa aaaatgcaag aattagtaat tttgaaaaat aaagaagctg
     2101 tgactacgag cttacaagtc gcagaaacat tccgaaagga acatagagca gttttaaagg
     2161 cgattgacga tctaaaagat gggcttgcac aaaagtatgc agacctattt tgggaagatg
     2221 tgtacatcca tccgcaaaac aaacaggctt atcgaattgt ctatatgaat cgtgatggct
     2281 ttactcttgc tgtattagga ttcaacaata ctcaaaaagt tttagagttc aaattgaaat
     2341 acattgaagc gttcaacgaa atggaaaagg caattcgcaa aaatacagct tttgacacaa
     2401 ttgaagacat gatgatctat caattagaag aaatgaaaga tgttaaaaaa gatgtttcca
     2461 tgcttaaaaa tactatgcga attagcggac aacaagagtt tgaaattaag caaaaaggaa
     2521 atacgaaagt tatggaagtt ctagggggaa aagaaagccg agcttatgaa gaaatcagca
     2581 aaaaagtatt ctcaaaattt tggtctgaat ttaaacgtac cttttcaatc ccaagatatg
     2641 gcgagttacc tcgtaagaga ttcgatgatg ctgtttcatt tattgaaatg tggttaccag
     2701 aaactgcgat tcgcatggaa atcgatcaac tgaacagaca acaaagactt tttggtgatg
     2761 aaaatgaata gagctgaagc gctaagaatc gggacggtaa ttgctaatcg ctggtggaga
     2821 cacaataaac caatcatcct aagccaacaa catattgaca agcaaaaagc atggcaacaa
     2881 ataaaaaagt gactccgccg gcaagcaaag agtcacaaag aaaacacatc ataaggagat
     2941 tttagcatat ggaaaaagaa ctttccactc tagatcaata tttgactgat cctagttggg
     3001 gcaaatcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacggatg
     3061 aagaactagc atgtgatcaa gacgatttgg gaaattttgt gagtatttgg gatcatgtct
     3121 atcttatcca tttatcgagg aagtccaaaa aacctgaata tatctatgtc atcgaagatg
     3181 gcttgattga tgcgctagaa gagtatgaca gagataactt gattgatatc tcttattacg
     3241 gatcaggtaa gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aaacaaaggg
     3301 acaacgactt ttgaaaaact ttttagtcgc aagttaaata aaatactcaa gaaaaaagga
     3361 aattttgatt atttatcttg ggctcacgca tgggagatta tgaaaaagaa tgatccacag
     3421 gcaacggtaa ctattaatga gtataaacac tacagagttg tttctggaac tcatcaagac
     3481 tttcttgttg aggaatataa accttttctt atggatgaaa ctgggactta tgtatctgtc
     3541 tcagtaacgg ttaaaggaca cacggaaacc gaattatttc ctgttttaga ttatcgaaac
     3601 caaccagttg ttaaaccaaa tgcaatgcaa atcaataact cattgaagcg atgctttgtg
     3661 aaagcattgg ctctacacgg actgggatta tatgtatttc aaggggaaga tattccaaca
     3721 ccacctagaa tcgatacgaa gaaattaaac atgctagaga cgattctaga agctttcaat
     3781 gagcagatgg gtaaagatat gaccaaaacc ttaatcgaat atgttaatga gcagacagat
     3841 aaattagggc tcttagctga taacgttgaa actattgaac agttaagcta tgagcaatgt
     3901 gccttgatgg agcgagcaat agcagctaag agaaaagaat tagataagaa gtgatatgag
     3961 tgtttaaacc attaatcgat tcatattcag cggttctgaa aaagttcaaa ggaaaagaca
     4021 taggtgcaac catcaatgag gaagtgaaca ttgatcgact aaagacgatg tatgacggct
     4081 acgatggtga tcgagttatt gaaattcgtt ttattgatcc tagacgtttc acagtacagc
     4141 aacgaaactt catctatgca ctcataggcg atattttcat cgatacaggc atgccaacgg
     4201 acttctggaa ggaattcttt tacttccgct ttgaaggtgt cacagggcgc aaaataagcc
     4261 tcaaagatga atcgaatacg actgtgagtg atgctaacgt cttagcaaat atcatcttag
     4321 atttcatctt tgaacatcat attcctttca aagaaggcta tgagatttta ccagcgaatc
     4381 aagaatatta cttctacaaa tgcatcacaa aaagagtctg ttgcatctgt ggcaaaacag
     4441 gagctgatat cgatcacttt gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc
     4501 attcagagta cacatttgca gcactctgca gaatccatca cacggagaaa cacaagatag
     4561 gtgtgatcaa tttcaaaaat aaatatcaaa tcaaagggat caagttaaac caggaaacaa
     4621 ttaagaaact tagaatagga ggataaaaaa tattggctga caacaaacga tactactatt
     4681 taaaactaaa agagaacttt ttcgatagcg acgagatggt tcttttagaa agtatgccag
     4741 atggatacat ttattctaat attcttctca aactttattt aagaagtctg aaacatgaag
     4801 gcaaactaat gtttaatgac aggattccat tcaattctac aatgcttgcg actattacaa
     4861 gacactctgt gggagttgta gaaaaagcag tacaaatatt tcgtgattta caacttattg
     4921 aggtattaga taacggagca atttatatgt ctgatataca aagttttatt gggaaatctt
     4981 caactgaagc tgataggaag agaaaataca gaaaagaaat tgaggaagca aaacagaatt
     5041 taataacaag tggacaaatg tcggacaaat gtccggacaa aactacacca gagttagaga
     5101 tagagttaga gaaagatata gagttagaga aagatataga gaatgtaacg ccttcgaaaa
     5161 aatcgaaggc taagcccgtc cgtcataaat acggagagta taaaaatgtt cttttgtcag
     5221 atgagcagat ggagaaactc aaaatagaat tccctaatga ctaccaagag cgaatagaac
     5281 ggctatctga gtattgtgaa tcatctggta agacttataa aaactatttg gcaactattc
     5341 gaagctgggc aagaaaagaa aaaagtgaat ctaagaacgc aagcagtgga tacaagcgca
     5401 caggaagacg agagaagctt cctgagtggg caatcgacca agaagcctat ctcaagaaaa
     5461 aagcgctaga acgagctaat agacaatcaa aagcaccatt ctaagaggtg ggaaattgaa
     5521 aatcgattat ctagaactaa ttaatgaaat agcaaagtat aaaactggtg aggaaataga
     5581 aatcctgaga gacgtatatg atcaactcga agaagctgga atcgaaggaa ttaagaatga
     5641 tcgttcgagt tggagtaagc tcagatacta tttcgcactt tatatcgata caacacaatt
     5701 aagaaattta gcatatacca aattactatt tgttgattgt gttaaaggct tgcaaaaaca
     5761 tcttaatgaa cttgagcagg tgtaatcaga tggacctaaa gacatttaca gcacagattg
     5821 aactaatgca tcaagaagct ttaagacaaa gtgtatcgta cgaagacaag tggctcaaca
     5881 cgttcca
//
LOCUS       DAPCMT010000149         5873 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPCMT010000149 DAPCMT010000000
VERSION     DAPCMT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.152"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(170..3319)
                     /locus_tag="RD743_002452"
     CDS             complement(170..3319)
                     /locus_tag="RD743_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDU0564245.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="RD743_002453"
     CDS             complement(3333..4550)
                     /locus_tag="RD743_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDU0564246.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..>5873)
                     /locus_tag="RD743_002454"
     CDS             complement(4540..>5873)
                     /locus_tag="RD743_002454"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDU0564247.1"
                     /translation="GYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGL
                     FDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAG
                     KKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKY
                     HGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSA
                     NWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAA
                     EGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKG
                     KNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPI
                     DMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1604 a         1250 c          983 g         2036 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc cca
//
LOCUS       DAPCMT010000150         5857 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPCMT010000150 DAPCMT010000000
VERSION     DAPCMT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.153"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(74..1078)
                     /locus_tag="RD743_002455"
     CDS             complement(74..1078)
                     /locus_tag="RD743_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDU0564248.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
     gene            complement(1149..1445)
                     /locus_tag="RD743_002456"
     CDS             complement(1149..1445)
                     /locus_tag="RD743_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564249.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1438..1731)
                     /locus_tag="RD743_002457"
     CDS             complement(1438..1731)
                     /locus_tag="RD743_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564250.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1877..2428)
                     /locus_tag="RD743_002458"
     CDS             complement(1877..2428)
                     /locus_tag="RD743_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDU0564251.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2602..2901)
                     /locus_tag="RD743_002459"
     CDS             complement(2602..2901)
                     /locus_tag="RD743_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HDU0564252.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(2928..3236)
                     /locus_tag="RD743_002460"
     CDS             complement(2928..3236)
                     /locus_tag="RD743_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDU0564253.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3353..4312)
                     /locus_tag="RD743_002461"
     CDS             complement(3353..4312)
                     /locus_tag="RD743_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0564254.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4456..5232
                     /locus_tag="RD743_002462"
     CDS             4456..5232
                     /locus_tag="RD743_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0564255.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(5330..>5857)
                     /locus_tag="RD743_002463"
     CDS             complement(5330..>5857)
                     /locus_tag="RD743_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDU0564256.1"
                     /translation="QKKIGTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDI
                     FPNILIEQLEWLKKKKIKMAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPN
                     PTIFLKASEQLRIPVEECLVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIY
                     DIKYLRQEVQVKFGI"
BASE COUNT         1765 a         1074 c          880 g         2138 t
ORIGIN      
        1 aaaaaaactg attctaagaa aattaaataa tttttctcaa aatcagtttt tctttgataa
       61 ataatatttt caattattta atcatttgaa aattgttagt cattccaata cgttgagaaa
      121 ttcttccatc tgtataaaca cggaattgtt ttccaccagc cattgttgta tttccattat
      181 catccgttac aatattaatt gaatagtagc ttccattttc atcttgcatt agatctttca
      241 tatattcatt tgtgctaatt aaattagtat ttgatgcatt tttatcagga gaatcataca
      301 accataaagc tttattattt agtacagttt ctttagcagc ttcccaactt gaaattgctg
      361 agctacttgt agattgtgtc gttgtagttg ttgcttgttg agtagattct tctactactg
      421 gagtttgagg agtttgtcca ttcacaatat caatgaaacc ttgttttaac tgttggttct
      481 gagtttcatt gaagtacatg tagtttttat cattgccttg attttgttgc gttaaataaa
      541 ctttaggttg tccattttga aagccaaaga aatatacatg ctgccctaaa tattctccag
      601 tttctacatc agaataaact gctactaaat tgaaatctgc atgaccagtt ccatcttctg
      661 accattgtac ggtaactggt gcttcattga ttgccatttt ccaatttcca tcaataactg
      721 cacttggtaa aggcgtacca tacagactta cattgttctg aggattgtag cttttatatt
      781 cttgacctaa tgtctttccc cattgtggga caaaaattgc tagttgagta gatttctcac
      841 tatcccacaa agtattttta acctcttcag aagaagattc aatgattgaa ctttcacttg
      901 tacttgcttc cacagattgt ttcatttctg ttgtagtttg ttcagttgat tgcgttttac
      961 tttttttagc ctcaattgtt gtatcagttt ctacagtctc tttagtcctc gtattctcag
     1021 atgtacaacc agtgaataaa atccccattc caattagtaa taacaaccat tttttcattt
     1081 tatttccccc tttcaatttc tccctcgcgt acttatttta tcagtcaatt aatctcaatg
     1141 gtgttcattt agttaatcct ttcccaagaa tgtcgatagc cagcatgaca acgatctctt
     1201 ccttgagggc tttcagatgg attaaaacta gtagaaattt cccttccaca tcctctacag
     1261 cgataagttc tcattatttt tgggctatta gtattatttt tcttagttag agctatgagt
     1321 ccaactatga ttaccccaac tgtcttaatt acttttcctt tatctatatt cggatgcgca
     1381 agcatatatt tatataacca atatccaccg taaattaaac cacctaatac aatcagctta
     1441 gccatgtact cgtctccttt ctttttgttt aattactttt tgaatccatc ttgcagagaa
     1501 accataaact tctgtcaatt gacggatatc tacagttttt tcctgttcta tcatgttttc
     1561 gattcttttt ctaactaact cacgatcgta cattttcatt ggaaaagaaa tctgttgccc
     1621 tcgaaaagca agataaaatt gatagagcaa ttctgaatcc agttccattt cctcaaaaaa
     1681 gtcattcata cttttataag agttactcaa acaagtttca tcaatctcca tctacacacc
     1741 ccctgttatt ttttacataa tgaaaatagc atattactgg gtacattgat aattcatata
     1801 acatgtatca tcacatgtat ttttaaagtc aaaaagcatg taaaactact tataagtaac
     1861 ataagaaaat aaaatgttat ttttcttgaa ataataattg actatctgcc aattgccatt
     1921 cattacgaac atgaatactt agtgggtggg ataacttacc ttctgcattt atcaatactt
     1981 tggtcatctt tttttgtttc gcactcaatt gatcgtaaat ggtcttcgct tgttcataag
     2041 ccacttgact cgtcgctgta atcgaaccac aagcaaaaat aatggtgttg attttctttt
     2101 ctgtgaaaac agttgttaat atttccaaag attgttcatc atttccacga ggaaataccc
     2161 gaggactttt cgcttttgta tacgaaaaaa gattcacagc aataaaacca gtaaatccta
     2221 attgtcggat attcttttcg attaataaca tactcaaatc attcgtaaat ggttctgtat
     2281 ctgttggacg aatagtaatg actaccgcta atttatcttc cgcttctttt cctttgatcg
     2341 cccattgtcg tttgaaaaca tagcgatggg tatgatgagg atctcgccaa acttctgttt
     2401 gtgtggtcac taattctttt tcaatcatct ctttttctcc tatgctaatt tttgttcaag
     2461 caattcaatc accattgccg ttataagtgt caatactagt tgaaaatatt tcatattctt
     2521 tctccttttt tcttcagaat ttgatgaatc gctgtaagga tatcttctaa acatttcgca
     2581 atcattctca aataatcgat cttattcatt cgttcctcca atctgactcc aaaccaatcg
     2641 tccttctacc agtaacccac gaacaaattc tcgctggctg gtaatatctg taatgaaata
     2701 aactacatag tcttttccat cttctggtgc gcaataaata taaagatgtg tcatcactaa
     2761 acgtggtaga gtaacatctt caggaagcat acccatttca atttcctctt tttccatcgt
     2821 aacggattca tagccccact cttcaggagt agtgactagc tgtgcacact tttccagtac
     2881 ttcgatagcc gtttctaaca agtcctttcg ttcctttctt ttagatatca gtattcttct
     2941 ggtaataaca tcgtttgata ctcacccgaa tcaatgaccc atattttttg agacactgat
     3001 ttcaaacgaa gcttaggtga aatcagatac tcttttcttg cttctggttc ttcttgacga
     3061 tgaaccaact tttgatttcc tttttccgta gaaaattcaa agatttgaag atagtctaaa
     3121 tgaaattgct tttgaacgtt ttgatcgatt agattccaac ataaagcttg taattccacc
     3181 ggcaaactgt ccattactcc ctttgtgaca taacgttgtc ctttattttg aaacatttct
     3241 tttccctcct ttgccataaa aatagcccaa tcttctatta aagaagattg ggcttcatgg
     3301 atataatata tatttgatat tcaatttgat tcattttata ctatctgatt tgtcaaatta
     3361 agctagacaa tatgtcttta tctatgcagc tcaaaaatac ttccaatggt gttcgataat
     3421 ttaatgattt tcttggaata tggtttcttt tcgaggcaac ggattgaata aaggtttcat
     3481 ccgtgtccct aaagtccatt gttttaggta agccatctcg tctaagcaag ccgtttgagt
     3541 gctcatttag ccctctttgc gaaggtgttc ctggatctgc gaaaaagatg gcaatatcgt
     3601 tcgagttact tatagatcgc cagttggaaa attcttttcc acaatcaaat gtcatggatt
     3661 taaacaagtg tttaggcatg gatttgaacc aatcatttaa cctgttttcg atatcttcgg
     3721 ctcgtctgcc tgtcggtttc aaagtaataa taaccttgga taaacactca accaatgtaa
     3781 taacagcgct cttgtgttta actcccacaa tggtgtcgac ttcgagatga ccaaattctt
     3841 tagtcaggtg tggatagaga ctgttccgtt catgaatcgt tctgcgaaag gcctgttttc
     3901 cacgcttttc ttgatgatta ttcggtttac gtttcccctt cataggtaac gactgtgtgt
     3961 caaaaaggcc ttttttaaac atccgataga gtgtacgaac agaacaatca attggaaact
     4021 cgccacggcc gataatcaca tcgggtgacc atccttgagc taccttttct tgaacatatg
     4081 ttgattgttc atcagataaa acggttgaac gtcgtccaca acgagattta ttttgtttgt
     4141 attgacggta ataatctagc actttttttc cttgtttgag aaacgaataa acattataaa
     4201 tggtttgtct agagcgattt aaactttgtg cgacaagagc gacaggtttc tcgatattga
     4261 aataagactc tatgattact agttcgtctg ttgtaagatg ggtgtaagtc atttgtactc
     4321 actccttatt aattctttgg tcggaactat tttgagtgta gcacaaatga tctttttagt
     4381 tgtctagctt aattttacaa tcgacgtttt aataaattgt gatataatga agtataactt
     4441 tttagggagt gtactatgaa ttttcaagat aatagaccat tatatttaca agtagaagct
     4501 tctatacgta acgatattcg tgaaaaaaaa tatcttccag gagagcaatt acctacagaa
     4561 gaagaattat gtaaaattta tggagtaagt aagattacca ttcgaaaagc ttttaaactt
     4621 ttagcagaaa gtggaacagt tgaacgatta agggggaaag ggacctttgt aactcaaaaa
     4681 aaagaaagtt taaaattagc tggagttcat ggatttagcg attctctttc tacccgagga
     4741 cataaaatta gatatactgt tttacattct aaaattataa aggctgatga gtttttagct
     4801 aataaattac aaatcaatgt aactgataga gtttacaata ttaaacgttt aatgtgggaa
     4861 gacggtgccc cgatgggaat tgataatttt ttcgcatcag aagaaaaatt tccaacttta
     4921 tttgaaaaag gtagccaata tacttcatta tataaacttc tagaagatga ctatgatgtc
     4981 aaagctacga gctctattat ggaaataaac ggtttatctg caacacctga gctttccgac
     5041 ttattgcaat gtatagtagg agatccgcta tttattttag aaaaggttgg caaagataaa
     5101 aataatcaac catttcatta ttctcaatcc acagttcgtt gtgatagaat tagttatgtt
     5161 attgaagtca cagaaaatat cactttagat acggtgcata aaaatgattc agatcctaaa
     5221 gtaatagatt gatattaaaa gaaatcatac ccaaagcaat caattttgat tagatttggg
     5281 catgattttt atttttacta atattctcta aatacatcaa aaaacttatt tatattccaa
     5341 atttcacctg tacttcttgt cgcagatatt taatatcgta aataacatca tctgctagac
     5401 ttacatcttg agaaaaacgt ttatctttaa ttgcaagaac cttcatattt gctcttttac
     5461 cagcttttat accattataa gaatcttcta tcaccagaca ttcttcaact ggaattctga
     5521 gctgttctga agctttaagg aatatagtag gattaggttt actttctggc aaatctcttc
     5581 cgctaattaa acaatcaaaa tatgactcta ttttacaagt ggtaacaact tcatttatag
     5641 tatccattgg actggaagaa gcaattgcca tttttatttt tttctttttt aaccattcaa
     5701 gctgttcaat tagtatatta ggaaaaatat ccaagtaatc aatcttttgc gatttccaat
     5761 atttagtaaa ttttcccttc aattctaaac tgttacatcc ctcattgtac ttttctatat
     5821 catcaaaaat acttgctgta gttccaatct ttttttg
//
LOCUS       DAPCMT010000151         5837 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPCMT010000151 DAPCMT010000000
VERSION     DAPCMT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.154"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(63..521)
                     /locus_tag="RD743_002464"
     CDS             complement(63..521)
                     /locus_tag="RD743_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0564257.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(534..1865)
                     /locus_tag="RD743_002465"
     CDS             complement(534..1865)
                     /locus_tag="RD743_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDU0564258.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1876..3318)
                     /locus_tag="RD743_002466"
     CDS             complement(1876..3318)
                     /locus_tag="RD743_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0564259.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3305..4069)
                     /locus_tag="RD743_002467"
     CDS             complement(3305..4069)
                     /locus_tag="RD743_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDU0564260.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4173..5048)
                     /locus_tag="RD743_002468"
     CDS             complement(4173..5048)
                     /locus_tag="RD743_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0564261.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5303..5387)
                     /locus_tag="RD743_002469"
     tRNA            complement(5303..5387)
                     /locus_tag="RD743_002469"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5351..5353),aa:Leu,seq:caa)
     gene            complement(5405..5476)
                     /locus_tag="RD743_002470"
     tRNA            complement(5405..5476)
                     /locus_tag="RD743_002470"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5442..5444),aa:Gln,seq:ttg)
     gene            complement(5486..5558)
                     /locus_tag="RD743_002471"
     tRNA            complement(5486..5558)
                     /locus_tag="RD743_002471"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5523..5525),aa:His,seq:gtg)
     gene            complement(5620..5704)
                     /locus_tag="RD743_002472"
     tRNA            complement(5620..5704)
                     /locus_tag="RD743_002472"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5668..5670),aa:Leu,seq:caa)
     gene            complement(5725..5795)
                     /locus_tag="RD743_002473"
     tRNA            complement(5725..5795)
                     /locus_tag="RD743_002473"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5761..5763),aa:Cys,seq:gca)
BASE COUNT         1773 a         1248 c          892 g         1924 t
ORIGIN      
        1 ttcaaaaaaa ttttgtaaat tcacccataa atatcaccct atttaacttt tttcatcgta
       61 cactaaaatt caaaacaact atctttaatc tttacttctt ggttttgttt taataaattt
      121 ccttcaaaaa tgcctggaga aactacaatg atcaccgtat ctttttgtgc ttgctccaac
      181 ttcttcaaat ctataatagc taacagtgtc ccttctttta cctcctggtt actttcaacc
      241 cgtatctgaa acggacttcc ttcgagttca actgtatcta aacccatatg gatcagtatt
      301 tttctgccag attcactttc gatcgttatg gcatgtcggg taggaaatat attatcgatt
      361 attcctttaa ctggcgcatg gatatatcca tcatgattcg ttatagcgta tccaactcct
      421 atcatttttg aagaaaagac ctcgtctttt actgctgtta atggaataat ttgaccacta
      481 gctacagctt ttagaatgtt tcttgtttta aagaaatgca ctttttatca cctctacttt
      541 aatgctggcc aatatttttt gtttactttg atcatttcat ctagtatttt ttttgcgact
      601 ctggcatttg gtacggtttg attcaatgta aatgcttgta aagctttttg ataagaacct
      661 tcaaagtaag catctactag taacttctct actgcaactt gtgcctccat caatccttta
      721 tgaaaatcac tgattggttt tcttagtgta attggctcca caccagttgc accaacatat
      781 gccggtattt caacaactgc atctgaccgt agattcggaa tcgccccttg attaggaacg
      841 attagcataa agcgacgacg atcatcattc aacaaggaaa tagctatatc gacgatatat
      901 tgtccatgct ccccaaaatt aaagttcaat acttcttcca caataccgcg gcgtatttta
      961 tcagccattt ctttggtatt tttctctcgt ccatccatga ccatattagc acgtgtatac
     1021 tctttgtcag tatgctcaac gaccatatca ggatataagt aatactctaa gtaattattc
     1081 gggatattac ttggaaaatt tttcgtgatg actgacatca tttgaaaggt tttttgccaa
     1141 gttttatctc ctatattaaa atcggcgact tgcatttctt ttgttattag tttttcaata
     1201 atctctggca taatttctcg ttgcaattct ttatcataaa tcgatgtata ccagccaaag
     1261 tggtttaaac cataatacgt aacgatccaa ttcttacgat cataaccgta attcactgca
     1321 atcgtttctt caattgaaat agtcatatca caggcattaa tcatttttac attcggatat
     1381 ttacgacgaa cagcttcaga aacgatcgac tctggatttg tataattcaa aatccatgct
     1441 ttgggggcat agtcttgtat atgatcgaca atttccaata aacctttgat tgagcgcatc
     1501 ccataagcaa atcctccgag tccacacgtt tcttgtccta ctaatccatg tttaagtgga
     1561 atcttttcgt ccttttctcg catctccaat cctccaacac gaatttgtga gaaaacaaaa
     1621 tcgcatcctg taaaagcaag ctttggatca tccgttgcac gaatgcgaat atttgaaaaa
     1681 ccttttcttt tcagcataaa tttaacaatc aagtacatat ccatatttct ttcttcatca
     1741 atatcatata aacgtatttc agataatggc aaccgttttt cattgttcaa aatggcttga
     1801 acgattcctg gtgtatacgt actcccgcct ccagctatag tgataatctg ttttttcatc
     1861 atcatctttt cctttctatt cttctaataa tttattgaat tctgccataa cataagtcac
     1921 ttctaagccg atgataattt gataattttt ttcagttggt ttaatgatcc cgcttatccc
     1981 taattgcttg attcgctgtt cctcgaccat ggtgggatcc actacctcta cccgtaggcg
     2041 tgtattacaa ctatagacat ccaggatatt ggcttttcct ccaaacgctt gtagcatttt
     2101 tgttgccata tattcataat ttttacttgc taaccctaac tctgtttctt ctaaggttac
     2161 ttcattgcca aactctgcct tagcttcacg tcctaatgtc tgaatgtttc tcttattgat
     2221 caaataataa aaagtaacgt aatacaaggc aaaaaagaaa attccgattg gaagaatcaa
     2281 aatagcattg gtagcgattc gaaaattgat gagataatca acaatacatg ctccgaatgt
     2341 aaaaccaatt cgtatattga ataagtaaca gacaatccct gctaaaccgg caaaaagtgc
     2401 atggatcacg tacaacatag gagaaagaaa catgaaacta aattcaacag gctcagtgat
     2461 atttgcaata aatgaagttc ctgcaccgct tcccatgatt cctttgacca tctctttatt
     2521 ttctttataa gctgctttat agatagctag acatatagct ggtataccaa acatcattac
     2581 tacaaaaaaa actgataaaa acagtccagc ctgaggatct ccgttgataa agcgtgtcat
     2641 ctctcctctg aaaacttcct tgctaggaga tgtatagctt cctaaatcgt aataaatata
     2701 cgtattcaac acatggtgca gtccaaaggg gatcagcaaa cgattcaaaa acataaatat
     2761 ccccacacct aatgctccca tatcaacgag cagttttgca aaattatcaa tcccaatttg
     2821 gatgagcggc cagataaagc ccaaaaaaac agctgcgatt gtttgtagaa tcatcaccat
     2881 agtcaatggg aatttttcgc ccgcaaaata agaaaaaact actggtaatt tttgttcttt
     2941 aaagcgatta tacgtccatg ccgctactaa accggaaata attccaccaa aaatccccat
     3001 attcacttct gggtccataa tttctaagcc ttttttcaag gtagcaatcg ctaaaaaacc
     3061 agtaagtgca ggtattcctt tgtccttatt tttgacaaaa ccgattgcaa ctcctatagc
     3121 aaataagata tccaagtttc caaaaacggc atttccagct gcaccaatta ctgggatgtt
     3181 cagcatatcg tcagctgata aacgcatgat caatcccgct aatggcatcg ctgcaactgg
     3241 aagtagcatc gctcttccaa gcttctgcac atttgattta acattcattt ttattcctcc
     3301 tctattaaat atcctcatat gttactaact caatcaaagg atcatttttc actttttgag
     3361 caaatgctga atgaattaaa aattcgactt ggtgggtacg aggatacaca aaacttgaac
     3421 catttaacaa ttcctgatcc aaatacgctg cgtgacacat gacttcgatc gttttatctt
     3481 ctctcaatat catgagtaaa cttgccaaat attcttccac actcgtattg atttgtcgtt
     3541 ttttttcatc agtttctcct acgacatcga aacaaggctc aaaataatcg acatgacgaa
     3601 cttcatcttt tcgttcaaag ttgctccgaa ccggcaaatc atattttttt gctaatgtaa
     3661 caactacctc ctgattttca tccaaagtat gagtatgatg atgactgtct aaatgagttg
     3721 gtatgatccc cgcatgataa attttttgaa tctgagcatt ccattcctga tacagttgat
     3781 ctttgtcaat gttaaatgga tgctgataat aagataagga tctgaactct ccgctttcga
     3841 taagagtatc gacagttttg aggagtggtt tgccacacgt caatgttaaa tggactccta
     3901 cacctagcct aggcatttcc tttcttagtt tcacagcatg ctcgaaaccc ggcatatttg
     3961 ccatcaatgt agttgatgtc aaaattcctc gctgataagc atccataatc ccataattca
     4021 caccatggct atagccaaaa tcatccgaat taaaaatcac ttttcccatt tcattctcct
     4081 tcttttttct actaaaatag gcgcaaccaa ataatcatag tccatttact atgtgtcatt
     4141 tggttgcgcc tatagatagt tacgccctta ttttaataga ttcttgctag atgaattgcc
     4201 aaatagatga tttcattttc ttgaatggca agttgaaatt gctgttcgat gatatcttta
     4261 atctttagtg ctgtacaata cgctttgcta tgtttaaatg tttcagtaaa aaaaccaata
     4321 ttttctactg gcgtttcttc ctgataaatc aagcgtttaa ccaggaattt gatatgaacc
     4381 attaagcgat gataagtcag tgattcttta tcataaacgt ttgaatagga tttttctaga
     4441 tgggacaaaa tcgtggtcgt catttctact atttttaagc tctttaaact attaggctct
     4501 cctagctcag cattaatgat atgcatagtg atgaatccta cttcatttga ccctaactcg
     4561 gtttgatatc gatgattgat tcgctcgacc acttctactg ctatatcata ttcatttgga
     4621 taaaggaatc tcatttctgg caacataatt gctgtgacat ctctatcttc tctttttcgt
     4681 tgaatagcaa aataaatatg atctgtcaat gtaatataga tatttgaatc taatggagag
     4741 gagatcctag ctgttatcag ttcaatcact tcttccgcaa aatccataaa ttcgattgga
     4801 atctgcgaga ctatctcact gaatcgtttg atcttttctt tagaatctaa tacaaataat
     4861 ttgtcagcat cctcaacatc aaatggctta ctcactttag cagaaaagcc aatccctttc
     4921 cccatcgcaa ttttttctat tccatcgtca tcgatcaata cagcattatg attcagcttt
     4981 ttcatcacat acaccgttaa ttcacctcct ctatatgata aaacaaaaca acttgcagac
     5041 tccttcatac atatttttgt ctccaaacaa tttttaaaga caaaaaacga cctaagaaag
     5101 aaccctttct taggtcgtgc ctaattgaat agtagcaccc tacataattt acaaaaatag
     5161 tttaacacat aataaaagcg ttatcaatat cttttttact cgttttatga taatacttgt
     5221 atcgaattga tttggaggta attacataaa aacaaaaaaa atatagcaac tctctataag
     5281 aaagttgcta tattgataat tataccggcg gccggggtcg aaccggcacg tcctcaacgg
     5341 acactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaaa tttaaaaata
     5401 ataactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc
     5461 gcttggctat accccaagaa taaagggcga atgatgggaa tcgaacccac gaatgccgga
     5521 gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tcataaaaga tgttatttaa
     5581 tttctcaata aaaaactaag atgaagacat cttagtcaat accggcggcc ggggtcgaac
     5641 cggcacgccc tcaacgggca ctggattttg agtccagcgc gtctgccaat tccgccacgc
     5701 cggcaagttg atttattagt actaaggcgg taaccggatt tgaaccggtg atgaaggttt
     5761 tgcagacctc tgccttacca cttggctata ccgccattat actattatca atctataaaa
     5821 taaagaataa attatag
//
LOCUS       DAPCMT010000152         5818 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPCMT010000152 DAPCMT010000000
VERSION     DAPCMT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.155"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(161..943)
                     /locus_tag="RD743_002474"
     CDS             complement(161..943)
                     /locus_tag="RD743_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564262.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1008..1844)
                     /locus_tag="RD743_002475"
     CDS             complement(1008..1844)
                     /locus_tag="RD743_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0564263.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1862..3271)
                     /locus_tag="RD743_002476"
     CDS             complement(1862..3271)
                     /locus_tag="RD743_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0564264.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3285..5108)
                     /locus_tag="RD743_002477"
     CDS             complement(3285..5108)
                     /locus_tag="RD743_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0564265.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1782 a         1261 c          948 g         1827 t
ORIGIN      
        1 tgaaactatc tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa
       61 aaaaataccc ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac
      121 aacggggtag ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa
      181 atacttcgtt gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc
      241 cattttcaag taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga
      301 tacctgttag attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat
      361 cagtatttat cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg
      421 tgtatgtttc gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg
      481 tctcattact cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg
      541 tctcattatt ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag
      601 tgtgcatagt taaaattccc acgcttttac taataaattc actataacca gttctttcaa
      661 tagagacttc aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca
      721 ttttgttgtt tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga
      781 atacagcttt actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc
      841 caagtagctc ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag
      901 aggtcgaaac attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag
      961 cacaaccaac aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt
     1021 gaaatatgca gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct
     1081 tgaacgtact ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact
     1141 gaagccagca aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata
     1201 aaaaactgta aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt
     1261 tcgggaaaca gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg
     1321 gtttcttcca tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca
     1381 gcctcctctt ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag
     1441 ccgatctcaa attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa
     1501 acaatgcctt tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat
     1561 agacttttat tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg
     1621 caaataatta gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca
     1681 tcagaaagtg aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct
     1741 ttctttagtt gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct
     1801 aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc
     1861 tctaagctaa gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta
     1921 ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct
     1981 tattgaagcc ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa
     2041 catctacacc atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa
     2101 ttcgatcctg gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat
     2161 tttcagtaat taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta
     2221 gtccagtagg gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg
     2281 cccggtagac accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat
     2341 ctgatccgtc atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa
     2401 aatcgggttt accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc
     2461 cacgatcatt caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta
     2521 agaaagacca gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg
     2581 aggtagcttg atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca
     2641 actgatgata caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa
     2701 agagttcttt ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa
     2761 ggatcatcgt gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag
     2821 taagcaatac ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc
     2881 cctgctcctc tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc
     2941 cctgattcag gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt
     3001 tattattttc aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc
     3061 ctaattcttt aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt
     3121 caaaattagc taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat
     3181 cctcctgata ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct
     3241 ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc
     3301 aatggttcgt gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg
     3361 ttggtcacta ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg
     3421 gcgttgatta ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag
     3481 ggttcccctt ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct
     3541 ttagaacgta gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca
     3601 ggtgaataaa tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta
     3661 gaaaagacag catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca
     3721 acaatgactg cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca
     3781 attagataag caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga
     3841 acaatattca ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca
     3901 aaggcatatc tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc
     3961 atggaagcaa ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta
     4021 attcccatta aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta
     4081 gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct
     4141 ggcccattga tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt
     4201 gcccaagctg tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt
     4261 aaccaagctg ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac
     4321 gttccaaacg gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc
     4381 ggcttagtaa agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag
     4441 gacatgatcc aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat
     4501 accggaattc ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga
     4561 tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct
     4621 gccccatagg ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa
     4681 atgatgtaag tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg
     4741 gcttttacca tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg
     4801 acactaatca atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt
     4861 gaagttccca cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc
     4921 acttggtatt gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct
     4981 ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt
     5041 aagctgacca cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg
     5101 taatccatta ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat
     5161 caacattgaa gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac
     5221 gttattttgc tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa
     5281 ttttttatct tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg
     5341 gtatatctgc gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa
     5401 accattccta cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat
     5461 aacggatcac acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta
     5521 aaggatggaa taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg
     5581 gtaaaaatca gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga
     5641 atgatttggg ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta
     5701 atctacatgt agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc
     5761 ataataattt tttctttttc atttctttta tattgttata acagtgaata acataaaa
//
LOCUS       DAPCMT010000153         5790 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPCMT010000153 DAPCMT010000000
VERSION     DAPCMT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.156"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(99..1229)
                     /locus_tag="RD743_002478"
     CDS             complement(99..1229)
                     /locus_tag="RD743_002478"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDU0564266.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="RD743_002479"
     CDS             complement(1877..3079)
                     /locus_tag="RD743_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0564267.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="RD743_002480"
     CDS             complement(3139..3342)
                     /locus_tag="RD743_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDU0564268.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="RD743_002481"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="RD743_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="RD743_002482"
     CDS             4879..5691
                     /locus_tag="RD743_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564269.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAPCMT010000154         5522 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPCMT010000154 DAPCMT010000000
VERSION     DAPCMT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.157"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..1008
                     /locus_tag="RD743_002483"
     CDS             <1..1008
                     /locus_tag="RD743_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564270.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="RD743_002484"
     CDS             1025..1174
                     /locus_tag="RD743_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564271.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="RD743_002485"
     CDS             1171..1617
                     /locus_tag="RD743_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564272.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="RD743_002486"
     CDS             1619..1756
                     /locus_tag="RD743_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDU0564273.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..2087
                     /locus_tag="RD743_002487"
     CDS             1794..2087
                     /locus_tag="RD743_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564274.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2084..2308
                     /locus_tag="RD743_002488"
     CDS             2084..2308
                     /locus_tag="RD743_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDU0564275.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2305..3330
                     /locus_tag="RD743_002489"
     CDS             2305..3330
                     /locus_tag="RD743_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDU0564276.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            3520..3593
                     /locus_tag="RD743_002490"
     tRNA            3520..3593
                     /locus_tag="RD743_002490"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3554..3556,aa:Ile,seq:cat)
     gene            4270..5432
                     /locus_tag="RD743_002491"
     CDS             join(4270..4546,4546..5432)
                     /locus_tag="RD743_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDU0564277.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT         1900 a          973 c         1133 g         1516 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata ataaaacgtt
     2101 cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt tcgtggggtt
     2161 agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca ttactggttt
     2221 tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa tgttctcggc
     2281 tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg
     2341 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
     2401 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
     2461 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
     2521 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
     2581 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
     2641 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
     2701 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
     2761 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
     2821 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
     2881 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
     2941 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
     3001 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
     3061 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
     3121 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
     3181 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
     3241 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
     3301 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
     3361 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
     3421 tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
     3481 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
     3541 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt acattaacgt
     3601 agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa gctaaagctt
     3661 ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga ttaatgaaag
     3721 gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat ttttaaatag
     3781 aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc gcaccccaaa
     3841 ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc attaaaaagc
     3901 ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg tagttaatta
     3961 gtgacttatt tttgatttta tagcactgat actataaaat atagatatca tcatattaca
     4021 caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata aaattcgttc
     4081 cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc aacgaaagta
     4141 tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcatgaat
     4201 cggctacact agactagaca gaaaaaataa ggtgtgtaga ataataaaaa acacactgga
     4261 ggatttttca tgtcaagacg tcaacgaaga acctattcaa aagaattcaa acaacaaatc
     4321 gtcgatctct atctcgctgg taagcctcgc gcagaaatta ttcgagagta tgagcttacg
     4381 ccttcttctt tcgataaatg gatgaagcaa gcacaatcaa cgggctcatt caaagaaaga
     4441 gacaacttaa caccagaaca agcagaattg atcgcactaa gaaagaaaaa taagcaactc
     4501 gaaatggaga atgatatttt aaagcaagcg gcgctgatat tcggacgaaa agacaagtaa
     4561 ttgatgccaa caagcataaa tattccatat cagcgatgtg caaaattcta aatatttctc
     4621 gtcaaaccta ctattatcaa gcgaaaccga tcgaaaatga gtccgactta gaagagatcg
     4681 ttcaggaaga gtttattcga aaccgaaagg cttacggtac ccgaaaattg aagaagtgtt
     4741 tagcaaagcg tgggcttcaa ctcagtcggc gccgaatcgg tcgaatcatg aaacgccgcg
     4801 gattgacatc tacctatacg atcgctcatt ttaaagggca acgaacagct tgtaatgaag
     4861 cgaaaacagc gaatgtatta gatcggacct ttacacaaga acagccattg gaagccatcg
     4921 ttacggatct tacttatgtt cgcgtgggga aaaagtggca ttatatctgc ttaatacttg
     4981 atttgtttaa tcgagaaatt attggttatt cctgtggtga gaagaaagat gcctcattgg
     5041 taaaagaagc ctttggacgg ataccgtatt ctttaacaga cgtcaagctt tttcatacag
     5101 accggggaaa ggaatttgat aaccaaacca ttcatgagat tctgaatggt tttggaatta
     5161 ctcgttcatt gagtaggaag ggttgtccgt atgataatgc cgttgtggaa tcaacctata
     5221 aatctgtcaa agtagaattc gtgcatcaat accaatttga gacactggca cagctacgtc
     5281 tagaattgtt tgattatgtg cattggtgga actatctacg cttacatggc acgttggcgt
     5341 atgaaacacc gatccaaatt cgacaacaga gattggcgaa gcgaatcctt gataatgagc
     5401 gcggatctga tacctctgga gaggcagcgt aattgaatga ttgtgcttct gccggagaaa
     5461 atcagatccg aggatgctca ttgtcaaggg caatcgtagc cataacaccg cagcattcac
     5521 aa
//
LOCUS       DAPCMT010000155         5313 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPCMT010000155 DAPCMT010000000
VERSION     DAPCMT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.158"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(137..1285)
                     /gene="nagA"
                     /locus_tag="RD743_002492"
     CDS             complement(137..1285)
                     /gene="nagA"
                     /locus_tag="RD743_002492"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDU0564278.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1361..2197)
                     /locus_tag="RD743_002493"
     CDS             complement(1361..2197)
                     /locus_tag="RD743_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0564279.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2381..3598)
                     /locus_tag="RD743_002494"
     CDS             complement(2381..3598)
                     /locus_tag="RD743_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDU0564280.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3747..3926)
                     /locus_tag="RD743_002495"
     CDS             complement(3747..3926)
                     /locus_tag="RD743_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564281.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3981..4064
                     /locus_tag="RD743_002496"
     tRNA            3981..4064
                     /locus_tag="RD743_002496"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4015..4017,aa:Leu,seq:gag)
     gene            complement(4304..5302)
                     /locus_tag="RD743_002497"
     CDS             complement(4304..5302)
                     /locus_tag="RD743_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564282.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1650 a         1080 c          843 g         1740 t
ORIGIN      
        1 ctttacattg ttacttattg aacaatagga aattaaagaa acttttgaaa cttatgtaaa
       61 gtgattttta ctagaacaca aaaaaacctt aaaaccaaac ttaagaagtt tagttttaag
      121 gttaacttaa attatattat gcgtggaaac gttccacgcc atctagataa gttgcttcca
      181 atgtcatatc agggttcaat acgataaagt ctgcatcgcg tccttgagcg atttttccac
      241 atgtatcatc gattttacag ctgattgctg gaatcagact agccatcatg actgcttgtt
      301 ctggtgttgc taatccccag tctacaacat ttttaattgc ttctttcaat ttcaatatac
      361 ttccagctaa gttgccagat tctagacgtg ctgttccttc ttttactact actggaaatt
      421 cgcctaaatt gtaatttcca tcaggcatcc caccggccat catacagtct gtaattaatg
      481 cgacatgatc atgtcctgct ttttccatta ggattcccgc agcatttgga tgtacatgat
      541 ggccatcaca aatcaattca gaaaagacat gttctaaaga taataacgca cccaccatac
      601 ctggttcgcg atgattcaat ccacgcatac cattgaaagc atggacaaat acacttgcgc
      661 ctgcttcaac agctttttgc gcttcttcta gtgtagcatt actgtgtcct agagaaacaa
      721 ctactccttc ttcagtcact tgtttgacaa actcttctac accttgacgt tccggtgcta
      781 aagcaatttt tttgattaaa ccacctgaag cttcttgcca ttcatggaaa gtatccaaat
      841 caggatcccc gaaataactt gggttttgtg cccctttgtg ttcttctgtg aagaaaggac
      901 cttcaaaata gattcctttg attttagctc ctggtgcttc ctgatacatt ttgccgattg
      961 tctcagctac atcttttaaa cgttctttgc ttgacgtcaa tgttgttggt aaaaatgaag
     1021 tcactccaca agataacaga ccttcagaca tcactttgat gccttctgca tcattatcca
     1081 ttacatcatg attcatatag ccgtgaatat gtgtatctac caaaccagga gcaatccatt
     1141 tgccgctttg gtcaatgatg ttgacttctc cctcaggtag ttctttggtg tattcaccaa
     1201 aaagtccatc ggtaatttca agatagcctg catgtttgac acctgacgct aaaaagaatt
     1261 tgtcagcata gataaaagtt ctcattctat tctctccctt tgtttttcta cctctaaagt
     1321 actcttattt agagaagaag tcaagaatgg tctacaccat ttacaaattt tttgaaattt
     1381 gtttcacagc ttgtgctaag ccttcataga atgctgctct ttctggttct tctttacgct
     1441 tgtatattaa gcttttaagc acaaataaat cattgaggta atagtagcta ttcttctctt
     1501 gagcccaata gattccttga ttcacctgtt ctaacgcttc gtcgatttca ttttgatgga
     1561 ataagaaatt tccataatta taaaaaattt gcgaaaattc actttttgat cgttcatcag
     1621 ctgattgatg cagcagttgg atgcttcgac tcaaatagta gcgtgcttca gccgtttttc
     1681 ctgccacacc atatattttt ccgatacaac taatcagttg tatttctgtg tcagaaacat
     1741 ggattctttc tttttggcta gagtagctta atgcttcttt caaataataa agggactgag
     1801 agatattttg acgcaacgtg aattcgcaaa caccgagata ataataatag cgttgaaagt
     1861 cagttgcttg atacagattt tcagaaatat cagaagattt tagtacttgt tctaacttcc
     1921 gataatcacg tttttggaaa caactatcca acagttcaaa taatttatcg tttgtccgag
     1981 tagtttttcg agagagattc atcacatgat caatcgttac ccctagccgt tgacaaatct
     2041 gcatcatcac caatacattc ggcagttcct cattattttc gatccgactt aatacacttt
     2101 gtgaacaaat atcatgcgaa agcatttttt gcgtcatttt ttgttctttt cgaatctctt
     2161 taatgatcgt tccaaaggac tcgattaacg tttccataag tatcctcccc aaaatatgcg
     2221 tattcgcatt ctattcgtat tttactttag gaagtcgcaa acttcaagaa aagacttgca
     2281 actaaataat atactttagt tatttaagaa aactccacta gaaagagagc ctcagtgtgt
     2341 ttgtctgtgg ccctcctact gaaaaatttg gtttttaaaa tcatttttgg cgataggttg
     2401 ctaggaattc tctcattttc ttagctgttt gtttcaaatc atccatttta ggaagataaa
     2461 cgattcgaaa atgatctggc tggttccaat taaatccacc accatgtaca agtagaatat
     2521 gatgttcatg taaaaagtcc aaaacgaatt tttcatcatc caaaatattg aatcgctttg
     2581 tatcaatttt tgggaatata taaaaagcag cttttggttt tacggctgaa agaccaggaa
     2641 tatcattgat tgcattatag atatattctc tttgttcata gatccttccg cctggcagga
     2701 gcagctcatc gacactttga taaccgccta gcgcggtttg gataatttgc tgagacaata
     2761 cattggaaca caaacgcatg gaggacaaca tattcagccc ttctatatag cctttgacgt
     2821 gtgacttatc tccacttagg accatccagc ctactcgaaa tcctgctaca cgatgagatt
     2881 tggatagacc gttcagcgta acaacgaaca aatcaggcgc taatgtagca atcggaatat
     2941 gttctagacc atccatcact aagcgatcat agatttcgtc ggaataaata atcaggttat
     3001 tttgtcgagc tacttcaacg atttcttcta ataattcttt tggataaagt gccccagtag
     3061 gattattagg attaatgata acaatagctt ttgtccgact cgttattttt gatttgatat
     3121 catcaatatc aggataccat tctgcctctt catcgcaaat ataatgaaca ggtgtgcctc
     3181 cagctaatgc aatagaagcc gtccacaacg gatagtccgg cattggaacc agtacttcat
     3241 ctccattgtc cagcaacccc tgcatacaca tcgtgatcaa ttcgctcact ccgttgccgg
     3301 tgtagatatc attgatcgtc acattcggaa aatgtttcag ttggcaatat tgctcaattg
     3361 cttttctagc agaaaagatc cccttggaat ctgagtagcc ttctgagtca cgggcattca
     3421 tgatcaaatc acgaatgact tcgtttggtg cttcaaatcc aaaaggtgcg ggatttcccg
     3481 tgttcaattt taaaatacta atgccctctt cttgcatccg ctccgcttct tctagaacgg
     3541 gaccgcgaac atcgtaactg acaccttcta atttgttgga tttttcaaat tctctcatca
     3601 cttagccaat ccctcatttc aaaaagttag aactttccaa attttttgtt atttactatt
     3661 tcaatatcat actccatcct agaaaatttt gccacttatt taggaaaaac gtaagtatta
     3721 aaaaaacaca aacaagcgtt gagaacttaa ttgtcatgat agtcattcca tagattcgct
     3781 gcccaatcca ttgcaattcc acaaatgatt cctagcataa aaccaagcaa aaaacgtccg
     3841 aatgcaagtc caatcaatac acctaagatc attcccagtc ctgaaaattc tccgtcatat
     3901 ttctccatct taataagtcc tcccattttt gagcttgaca actatgtaaa acctcgctat
     3961 cattagtgtg ttattattac gcagatgtgt tggaattggc agacaagcat gattgagggt
     4021 catgtgagcg ttagctcgtg tgggttcgag tcccatcatc tgcactacaa ctcgtgaagt
     4081 taattttgct tcacgagttt ttttgtttga acgtttcttt atgccattat atacatatca
     4141 ctgtatcctt tacaaaaaga acgtacaatc atgtaaacag cctgaggtga gtatatgaag
     4201 gtcggaaaca agctactcgt agaaacttgc aagtgttaaa acaattaatc gaaaaacgag
     4261 taaaatccct cacctaatac taaagcgagg gattttttgt atattatctg tttatcaccg
     4321 ttattataaa ggtacaaata ccaagcacgg ttaatccaaa ttgcataaat tcattttttt
     4381 gtacactcat gcttttcatt agctctttaa ctgccattag tactaagatg gcgataacaa
     4441 cataaagtat atagtacgtc cgcattccgg aagcccatac tgtgggagaa aatcccataa
     4501 ttaaccttga acaaaatccc attatcatca aaataactaa aaaaatagaa gttttcttat
     4561 tgtcaaaact taaataaatt ccaacaatta aacatagtaa taatgccaag actactaaag
     4621 tagctatcca agtacctgga taaaaaagtg acagtttcgt tcctaactct gtaaagagat
     4681 tattcaggtt actactactc caaatcattg cccttttatt tccattgacg taagtaaagc
     4741 tttgacccat tgtattacca aaataaataa tcaggtttaa gaaaaacggt aatgcagtca
     4801 atatacgcac ataatagttt tgacatttcc tatatgttag aaggaaaatt aagaaaaata
     4861 gcaataaaaa taagatattc atgtctaaaa aaaacggctt tccaaatgat gaaataccta
     4921 aatctaactt attaagaaag ttgaaatttt tatattcagg aaaccattta ttaatttcat
     4981 gtgcagcacg taatgcattc cctggagttg tcaaagagaa aattaattct gctaagctta
     5041 ttattgaaaa tactgataac ttataattat atcgtccatt gacaatcaaa tataaactaa
     5101 ctatacttgt tagaacaaaa aaacaggcgt tgacttgttc ttgattagtt aaaaatatca
     5161 ataaaggaat acttacccaa tatatttttc tatttatttc ttctcctctt aagagttgaa
     5221 aaaacggata aaaagttagt aaactaaatg ctactggcca ctgatagttc aaggtggttg
     5281 ctctccaacc ggtttccccc attactgtca atg
//
LOCUS       DAPCMT010000156         5301 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPCMT010000156 DAPCMT010000000
VERSION     DAPCMT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.159"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..333)
                     /locus_tag="RD743_002498"
     CDS             complement(<1..333)
                     /locus_tag="RD743_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0564283.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIV"
     gene            complement(449..1363)
                     /locus_tag="RD743_002499"
     CDS             complement(449..1363)
                     /locus_tag="RD743_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDU0564284.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(1454..1882)
                     /locus_tag="RD743_002500"
     CDS             complement(1454..1882)
                     /locus_tag="RD743_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDU0564285.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(1887..2261)
                     /locus_tag="RD743_002501"
     CDS             complement(1887..2261)
                     /locus_tag="RD743_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0564286.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            2547..2732
                     /locus_tag="RD743_002502"
     CDS             2547..2732
                     /locus_tag="RD743_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0564287.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            2744..2992
                     /locus_tag="RD743_002503"
     CDS             2744..2992
                     /locus_tag="RD743_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564288.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            3023..3202
                     /locus_tag="RD743_002504"
     CDS             3023..3202
                     /locus_tag="RD743_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564289.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3245..3715)
                     /locus_tag="RD743_002505"
     CDS             complement(3245..3715)
                     /locus_tag="RD743_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564290.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3802..4500
                     /locus_tag="RD743_002506"
     CDS             3802..4500
                     /locus_tag="RD743_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDU0564291.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4678..5019
                     /locus_tag="RD743_002507"
     CDS             4678..5019
                     /locus_tag="RD743_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564292.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            5012..>5301
                     /locus_tag="RD743_002508"
     CDS             5012..>5301
                     /locus_tag="RD743_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDU0564293.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1761 a          828 c          956 g         1756 t
ORIGIN      
        1 aacgatgtaa gtctcaggta tctttttctt tatagtgttt aagatattat tcgtaggata
       61 atatgtagac tccttaactt gtcttttgta aataactaac cattcttcga caagctcatg
      121 aaatgttaga tcaggcttct gaagtgtttt ttcgtttaat tttatatcaa tttttttatt
      181 taactctagc aatgccactt tctgagtttc tcgagattta tttttataag taatgctcac
      241 tttttttctt tttctagttt ttggatcaat atatcgctca ttatatttat atactttttt
      301 tccgttttta tcagttttgg tttcaatcca cattgttttc atctcctatt tgttgctata
      361 ataggaatgg ataagtaagc caattatagc aggtttattt ttcatcacgt ccacaaactt
      421 tggcgaggga gggggcgtgt ttttttgttt agtagctacc tttccatgaa gtatctgcga
      481 agaacacttc aaatgttgtt ccatcatttt tcacttcaaa atatgctgtt ccttgtgcgg
      541 atttacccgg ttggatggtt tccgaaaagt aatctctact agctaaatca gatttaatat
      601 ctgatgaatc ataaaattct aaataacttg aacttgcatc aaaaggtgta gtccctgtgt
      661 tttctacaag aaaatctacc tttgcgtaaa ataggccttc tggtttatag aaatcatcac
      721 cgtaatcttt tgatacagac tggacagtta cgttaataga gttaccattt tcagaagtaa
      781 aaggaactgc ttctcctata ctatgtgcaa ctgatgccgt ctggggttct gaagacgaag
      841 ttgtagttgt agttgtagta gagcttgaat ctttggattt tccatcttta tctaagaaag
      901 ttcgttgatt ataatataca acctgttcag tttttggact aacccaaatt aacattgttt
      961 catcatcaga atcagttgaa taagtatatt ggagcatctc taattttttt atatcttttg
     1021 cactatttcc atgttcaatt aattcagagt gctctttata ataatctcct tgtgcatcct
     1081 ttgaaataga tacaattaat tcatcccgtg tgatagcatt gtctaattct ttgtaaacaa
     1141 aatcatttgt ccatttacgt aacggtgaac caagtgtctg ataaacttga gttaaatcat
     1201 caccaacttt tattttatga acgtctgatg aagttagttt ttttgtttca tattcagatg
     1261 ttgatgaaac agtattttct ttagtgctag cagaattagg actagtggag catccgctta
     1321 ataccaagcc taaaaaaagc aacccaaaaa caattttttt catttttaat tcctcacttc
     1381 tatgatatga tttttttgta ggatcttaga aataaggttt ttagtccgtg ttgcagcacg
     1441 gacttttttt acattatata agaaagcgat aagcgctttc tggaagtccg taaagattct
     1501 ttaattcctc aattcgttta ggatattgat cattgtcttc tttgtataga gaaacaatga
     1561 gattagcagc aaagcaatta gcttcgcttt cagatttgct tctagatgtt cttgttgata
     1621 catagtaact ggataagcca cgatgaaaaa tagcgtgacc taattcgtga gcgcaaatgt
     1681 agaatctttc ctcagagtct ctcagttcat catttaagaa gattattgca cgacctctaa
     1741 tttcttgaaa ctgtcctttg ggattctcga taaaaggaac gtattgaatt ttaatgccca
     1801 tcttttcaca aatataaaaa ggattagcgg actggtattt ccgcttcaac tcctcgacta
     1861 aattaatcgt atccatctcc ataagctcac atctttttgc ctttttcttt gtcttctttc
     1921 acaatatccc agaaggttcc aataaggata tcttttacgc gttggacctg ttcaggcgtc
     1981 agtgtttctc cgccataaga catattgaca tttgaatcta gtaatttatc aagttcaact
     2041 acttcatctt ttgttgccca gtcaggaact tggtttctac cgagtaaata atcagtagtt
     2101 acattaaaat aatctgctac aagttgtagt cttttagtac taggagttct atttttccat
     2161 tgataaatcg tgtttttagg tatatttaat tcttcttcca attgagttat acttatgcct
     2221 cttttatgag ctaattcctt tattctatct agtaaattca ttttcgcacc tcacaagcta
     2281 cgaaaacact aataaaaaaa ttagttaaag gtgttgacaa ctaataaaag tgttagtata
     2341 ctgttttcgt aagctaaaat attagctaaa aagacttgat aactaataaa cacttcacgg
     2401 tcggcaaact ctgaaatgta aattactagg catttctgtg tcttatttag ctatgtctat
     2461 atactaataa aaatattagt tattgtcaac gaatattagc taatttttta gcttacagat
     2521 tattttttta gaaaggagct atttttatgt ctgagaattt agacttaaaa attcgagcgg
     2581 agatgagaaa aagaagaatg actttcaaag aactagctgc gcttatcggt atttcaggag
     2641 cttatttatc agatattcta aacggcaatc gtgatggaaa gaaagcacaa cagcatatcg
     2701 aaacagtgaa aaaaatattg gacatccgat aggggggtag gcgatgacaa aactaaaaaa
     2761 acaagatttt gtaaaaaaat acaattattc tccatctact tatcaacgtc gaatgtcgga
     2821 actaaaaaat acagcaattt tctcagcggc gtatgaacgg gtcacaggac aagaagtttg
     2881 gatcaataca gaattatacg ataaattttt gtctttcaaa tcctataaca ggttacgcac
     2941 aagaaaggta acacctaaag aatttatcga gaagcattta gttgatttat aaaaaacaga
     3001 acattttgag aggtgaaggt taatgggtaa attcaacaga gcattggtat tcagcgcacc
     3061 gctaatcatc tacgctttag gactttgggg aagcaggcaa gcgttgatag gaacgattgt
     3121 ttacatggtt tggattttta tggggcttga tgaagctgaa gctgagtaca gagcgaaaaa
     3181 gccaaccgag ggggctgact aaaaatgaat agaaaagaga aactagaatg tatattacta
     3241 ttactcagtt taattctttc actaatttct ctattgggaa gtttgtattt ttgatatcaa
     3301 caattttgtg gatttgggtt ctatttagag taaatatcat ttttgtttgg tgattaagga
     3361 gtttattaaa attaattttc cctacttcaa atgcaaggta acaagatata gcacttttag
     3421 gaggaatatt tagcggtaca gcttcagagt atattctatt atcatcgact gatcctcctt
     3481 tagcaattaa tttcttaaat ggagtagctg accatctgcg gtttaagtta gcggatgaaa
     3541 gttctaaatt aactaacata gctggttctg ttgaatagtt agaaatgatt acttttgtat
     3601 aaatcatatc tgcagcgaag taagaagcat taagttcaac ttctagttga gggcgcattc
     3661 cttttatttt tatccctgtt aaaaacgtac tcaatataaa acttattatt gacatccatt
     3721 gaaaaattgt taagtgaaaa aaattcaaaa taaacaccac cagtttttaa ctaaattata
     3781 ccaaaaagga gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa agaagctgtg
     3841 actacgagct tacaagtggc agaaagcttc gagaaaaaac atcaacatgt tttaagagat
     3901 attgatgcac taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa
     3961 ccagattcat atggcagaaa tcgacgagtt ttcttcatta gtagagatgg atttttcttg
     4021 ctggctatgg ggtttacagg aaagaaagct atctacttca aacaaaaata cattgaagca
     4081 ttcaacgaaa tggaagatgt tattcgcaag aatactgttc ctcaaacaat tgaagatatg
     4141 atgatctatc aattagaaga aatgaaagat gttaaaaaag atgtttccat gcttaaagat
     4201 actatgcgaa ttagcggaca acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt
     4261 atgggagttc tagggggaaa agaaagccga gcttatgaag aaatcagcaa aaaagtattc
     4321 tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc caagatatgg cgagttacct
     4381 cgtaagagat tcgatgatgc tgtttcattt attgaaatgt ggttgccaga aactgcgatc
     4441 cgtatggaaa ttgatcaact gaacagacaa cagagacttt ttggtgatga caatgaatag
     4501 agctgaagcg ctaagaatag ggacggcaat tgctaattgc tggtggaaat actataaacc
     4561 aatcatccta agccaacaac atattgacaa gcaaaaagca tggcaacaaa taaaaaagtg
     4621 actccgccgg caagcaaaga gtcacaaaga aaacacatca taaggagatt ttagcatatg
     4681 gaaaaagaac tttccactct agatcaatat ttgattgatc ctgattgggg caagccgaaa
     4741 attgaggaaa caagtggtcg aaaaatcaga cgaaatcttt tgacgaatga agaactagct
     4801 tgggatcaag atgatttagg caaccatgta actatttggg atcatgttta tcttatccat
     4861 ctatcgaagc attcgaataa acctgaatat atttacgtca tcgaagatgg cttgattgat
     4921 gcgctagaag agtacgacag agataacttg attgatatct cttattacgg accaggtaag
     4981 aaatacattg ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga caacgaactt
     5041 tgaaaaactt tttagtcgta agttaaataa aattctcaag aaaaaaggaa attttgatta
     5101 tttatcttgg gctcacgcgt gggagattat gaaaaagaat gatccacagg caacggtaac
     5161 tattaatgag tacaaacact acagggttgt ttctggaact catcaagact ttcttgttga
     5221 ggaatataaa ccttttctta tggatgaaac tgggacttat gtatctgtct cagtaacggt
     5281 taaaggacac acggaaaccg a
//
LOCUS       DAPCMT010000157         5261 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPCMT010000157 DAPCMT010000000
VERSION     DAPCMT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.160"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(1274..1939)
                     /locus_tag="RD743_002509"
     CDS             complement(1274..1939)
                     /locus_tag="RD743_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564294.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2119..4101
                     /locus_tag="RD743_002510"
     CDS             2119..4101
                     /locus_tag="RD743_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDU0564295.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4105..4743
                     /locus_tag="RD743_002511"
     CDS             4105..4743
                     /locus_tag="RD743_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0564296.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4771..5199
                     /locus_tag="RD743_002512"
     CDS             4771..5199
                     /locus_tag="RD743_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564297.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1746 a          799 c          957 g         1759 t
ORIGIN      
        1 aaaaatgatt tagaatatca aaataacaaa aaagaggact acctgtattc gtcctctttt
       61 ttgaccagat tttgaccagt ctaatttaca ttctaatata ttcatatgag ctaatttttt
      121 gattaaatgg taagtttttc cttgtttgtt ttagtttaaa ctagtgtgta atttaaaaat
      181 ctatatggaa gggtctgtgg ggtaatactt aaataaaggc taggaacctg ataacatcaa
      241 ggttcctagc ctttttataa taaaatgaag cgaaaataat ttcttttaaa gagccgagat
      301 tatcagctat ataagagtga atttgatgca aaaaacagag ccgaaacagt cttggtgttc
      361 gtagctctgc ttatatgttt cgaatttaag acaaagagtt ggcaaaagat gttgataagg
      421 aagataagat aactgtgtta tataaactag gtaactaact gtaacaagct aattttaaga
      481 gattcttatt agaagacgtt taggaatata actaagaaaa tgattataca ggtaattgtc
      541 atttcaataa tgttttctaa ttttcgcgtc tttgttttaa gaaaaaggcc agaatcagtt
      601 ggaaaaaagt tcgtacgata gtgaagaggg gcaaaatttc ctactaatat tcccactaac
      661 aagagataaa acggccaggt tggtgtgatt cctctataaa aaaatgccca tgctaccgaa
      721 atcccataga ttaacaactg aacaattttt acaatgaaaa aattgttctg ccattactca
      781 aagtctcttc ctccttgtat cgtttatttg tttcatttta tcattatgat aggcgcttga
      841 acaatccatt ttgtatttta ttgagtactc ttggataaac taccagcgat tatcatattt
      901 atttatgtta ctgtaagcca tcatataaaa gtaaatgagc agatcataga aatataaaaa
      961 agaactaaaa ttgatctaaa ttatgtgcag cttgactacg taccaatcta tattacttac
     1021 tagattggtc gttaatctat tcggatcgaa tgagcaaaat atttttgtga atgtccatcc
     1081 ttgattatga ttccaaaaag aatcattggg aggctgtggc gtaaatatta tactataagc
     1141 cagaaatacc tatcattctg aactttttta tttaggaact ttcatctcaa ggctttttca
     1201 attaatttgt acccctgttt tttggataag atcatgtgca ttgatttttt ctatctctac
     1261 gttcatctcc tacttaatgc ctatctggct cctgagggct tctttttaag gcgtcgtcat
     1321 agtcttggct gaggatttct tggtaatctg agggaatggc ttcccaagtg agcaggcgaa
     1381 cgttcatgac gtgttttcct ttgtgtttga ttacagcatg gtttttctct ttttctacct
     1441 ctccagcgtg aaaagacagc catgtaccat ggccaaagat atcattacat acccaacgtt
     1501 cgatggaatt gtccacgtat cctacaggga gtgtggtata gccgatttgt ggtttcaaaa
     1561 aaggattgaa tcgatcgatc gtttcatttt tgtattgata attgataaga aaaagataga
     1621 ccaaacaaaa gacgagcaga aagaaagata gtgtcttttt gagtgagaag ggttgttttg
     1681 atgaataaga tttcttgtta acaagaggtt catctggcaa tgttggttct attgattcgg
     1741 tagctgattc aatagaagga aaatcaatcg gcagacctaa agtatgatgt aaaagtgaca
     1801 gcatttcaag gtctggcatt gtacgagaat tttcccaact agaaatcgtt ttatcggaaa
     1861 caccaatttt ttcgcctaat tctttttgcg taagcttaca atttctgcgg taagttcgta
     1921 ttttttctcc ggtgttcatc ttcttctcct ttcgattcta cagtatatag atttattcta
     1981 catgacctat acacttgata gatacgtttt tttgatgaaa aacggtatga ttttatcaaa
     2041 gaatcattta agtaagggag agtggtgata atcaaaaaaa agttcacagt tttactcaca
     2101 atcatagtga ccggtatcat gagtggtttt gccttggtta gtataaataa acaggtactc
     2161 tatcggacaa atgaattttt ccatcaaaca gatacttata ctccgattcg attgccacaa
     2221 cattttattg ggcaagaaga atatgagcag ctaatcatgg tatttgaaaa tgtctcatca
     2281 gaaactgagc tttcttatat gaaaaaaaca gtaaatgaat ccaatgaagt agatacatct
     2341 tggaacttca agatacagcc aaagcttgaa atgacattgt attatcaagg ggatgtcaaa
     2401 tcttctcacg agaaggtacc ctattctaat acctcctttc gcgtaacgtc cattgaagaa
     2461 tcgatcaaaa taaaagattt tgaaggtgaa tttttcttga tgacgagaga aaaagaagcc
     2521 tatgaacggt tcatttcact attcactgat cgattcaatc aagcatttca cactgattat
     2581 gccactaata tttttgaaga ctttgaaaat agctattcgt attcggttat ttcaaataca
     2641 gacgtatata attatcgagt gtatttaact attggattga ttcttttatt ccttttgtta
     2701 tcatcctttt tcttattgat gaataaagaa atcagtttat tgttgaataa tgacttttca
     2761 ttacatcaag tattgtggtg tttattgggg aaaaagaaaa tcattattac attcataatt
     2821 gcaggaatca ttggtggttt tatttttatg tattataagg agcaatttct ttatttgttc
     2881 cagatgtacg ttttcttgtt cagcggaata tatggtatct ctttgatttc tcttttatta
     2941 gttgaaaaaa tcgcttcgtt aagagaagcg ttcaactata tccaatacat tctttttaca
     3001 tttggattgt tgttttctct tcttttgacc gttgccaatg tcgaattagc aactatcgtt
     3061 ttttcttctt cacagttagc tgtttcccat gaaccaccaa aagaaatcgc tggtgaggac
     3121 tatcatgtat tttatccagt aaccatcggg aaaaaccacg tggattttat ttattccaat
     3181 cgttttgcat cagcagcaga tcaggaatta tatgaaactt tgaataaaaa cggaagtata
     3241 ttagtcaatg tatcagatta cttgaatgaa gaaaatgaac agtttggcag agggataaaa
     3301 ataaatctga attatcttaa gaaatttcct ttgattgatg tctctggaag gttgatccaa
     3361 ataaccgaaa aagaaacgca ccctgttata ttgatccctg aacgatatag acatacagat
     3421 ttaaaaaatg acctggcgca aataacagaa tattatcaag aaatcagtga aaaagaaccg
     3481 aaatttctat ttataaaaaa caatcaaccc atttatacat ttattccttc tatcccctgg
     3541 atcgaacatt atccggttgt tgaaatactg acattgaaaa acagtatgta tttggaaaga
     3601 aatattctca gcggagaaat ctatccaccg ttaaagatca agataggctc tttgtcgaaa
     3661 gaaaggttat ttgagctgat tgaagaaagt cagttacgtg ataacttgca gttatcattt
     3721 ccgtatactc agactgaaga gatagctgtc aaagtgttaa gcagatcatt tcattacttg
     3781 atacagatat ttttactcac tttcttttct ttcttcttgt tgagctatgt catgttatgc
     3841 atatattttg tctacaattg tcggaaaatc gctatctttc gttcaagtgg ttattcgctc
     3901 tttgaaacgt ataaagattt cttcatgatg aacctcatca aatggggaac gacaagtgta
     3961 atcttccttt ttcttataga aagagagcct aaatatcttt ttaatatttt tttctttagc
     4021 ttcattgatt ttattctttc agttgttttt attttgtcta cagaaaagaa aagtcagtta
     4081 ttgctgatga atggaggtta acaattggag ccgttgatag tgataaaagg tgcgaaaaaa
     4141 atcattcgaa acaaaacgat tcttcatttg gatcgtttca ctgcggatgc tggggatctt
     4201 gtgactgttg ttggagaaag tggagcaggt aaaagtacgt tattgaatat attaggattg
     4261 aatgatcagt tcagttcagg acaattctat ttgttcgatc aagagacgaa acaattaact
     4321 gggagagaga agcgcttgat aaaaagaaat accatatctt ttctttttca agattttggg
     4381 ttgattgaat atgaaacaat cgattttaat ttagatattg gattgaagtt ttcgaattta
     4441 aatgtttcag ataagcgaac agcaaagtat gaggcattga aaaaagtaaa tataaacaag
     4501 aagctaagta cgcctatcta cacattatct ggtggtgaga agcaaagaat cgcctgtgcg
     4561 agagtcttat tgaagaatag caaaatcatt ttagccgatg aaccgactgg gtcattggat
     4621 aaaagaaata aggataaagt agttgaaatc ttatcgcaac aagcttccca aggtacgtta
     4681 gttatcgtag tcacgcatga tccatatctg accaaaatag gaaacaagaa gatcatgctt
     4741 taaaaatcaa aaaggaaagg atgtcgtttt atgaaaaaag cagttattat ttgtgcacta
     4801 gggataggtt ttgttggagg aaacgctatg tcatctgaag tggaagcaaa ttcagttcac
     4861 ggtgaaatct attacaaatc tttaaagcca ggagagattc attttgatgc taaaggtaat
     4921 attattaagt caaatttacc agtagaccgg gaaatttcta gaacagcaat taatcatagc
     4981 tctggaagat gggtttatta tagtgataca acttgtacga gaaaaacagg tcattctaac
     5041 cactatagtc gtgtctataa tcgtcacggt gcaaaagcga aagttggaaa ctctattcga
     5101 tcagcaaatg caacaaaagg agtatgggcc tatgcaagtg cgacaggaaa tagaactgac
     5161 acattctcat gttggtacaa tccaaccggt tggtattaag tatccaaaag tttatataaa
     5221 atagaaaaaa catataatga aacagcgtta aaagttattt t
//
LOCUS       DAPCMT010000158         5240 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPCMT010000158 DAPCMT010000000
VERSION     DAPCMT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.161"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            175..519
                     /locus_tag="RD743_002513"
     CDS             175..519
                     /locus_tag="RD743_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDU0564298.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="RD743_002514"
     CDS             520..939
                     /locus_tag="RD743_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDU0564299.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="RD743_002515"
     CDS             complement(1031..1381)
                     /locus_tag="RD743_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564300.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="RD743_002516"
     CDS             complement(1347..2567)
                     /locus_tag="RD743_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDU0564301.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="RD743_002517"
     CDS             2933..3499
                     /locus_tag="RD743_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0564302.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="RD743_002518"
     tRNA            3644..3731
                     /locus_tag="RD743_002518"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..4631
                     /locus_tag="RD743_002519"
                     /pseudo
     CDS             4065..4631
                     /locus_tag="RD743_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            4744..>5240
                     /locus_tag="RD743_002520"
     CDS             4744..>5240
                     /locus_tag="RD743_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDU0564303.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT         1914 a          885 c          990 g         1451 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaacgaga gtataaaata ttttgtgtaa atgaaaaaat ccatacaaaa
     4681 aaggaagtcc cttctgtaga ataaagttaa cgacaaccaa ttcacagaaa agaggacttc
     4741 cctatgaatg attttactac agaaattgtg caaactctag tcactaaagg cgatttaaat
     4801 gaattattcc gttcgcactt agaaaaagcg ataaacacac tcctacggac tgaattaacg
     4861 gcttttttag attacgaaaa atatgatcgc actggtttta attcaggtaa ttcgagaaac
     4921 ggttcttact ttcgatcaat caaaaccgaa tatggtgaat taacattgga aatacctaga
     4981 gatcgtaatg gtgagtttaa acaacaaact ttaccagcct acaaaagaac aaacgataca
     5041 ttggaaacca ctattatcca tttattcgaa aaaggtgtta cgatgtctga aattgctgat
     5101 ttgatcgaaa aaatgtacgg tcatcactat actccacaaa ccatgtccaa catgactaaa
     5161 gttctgactg aagaagtaaa tgcctttaaa tccagagcct taaatgataa gtatgtcgct
     5221 atttttatgg acgctactta
//
LOCUS       DAPCMT010000159         5239 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPCMT010000159 DAPCMT010000000
VERSION     DAPCMT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.162"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(406..780)
                     /locus_tag="RD743_002521"
     CDS             complement(406..780)
                     /locus_tag="RD743_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0564304.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="RD743_002522"
     CDS             complement(803..2203)
                     /locus_tag="RD743_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HDU0564305.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLSEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="RD743_002523"
     CDS             complement(2207..2617)
                     /locus_tag="RD743_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0564306.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="RD743_002524"
     CDS             complement(2635..3126)
                     /locus_tag="RD743_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0564307.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="RD743_002525"
     CDS             complement(3136..3927)
                     /locus_tag="RD743_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0564308.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="RD743_002526"
     CDS             complement(3920..4693)
                     /locus_tag="RD743_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0564309.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5239)
                     /locus_tag="RD743_002527"
     CDS             complement(4849..>5239)
                     /locus_tag="RD743_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0564310.1"
                     /translation="MDSDSCEFCLNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYL
                     YEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFS
                     EIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1676 a         1058 c          689 g         1816 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcagacag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagtactt tttctatctc attgaatact tttttggaat ctaaaaagcg
     5161 aagcttagta ctagcgcctt caatagtaaa gagtttaact aattcattta agcagaactc
     5221 acagctatcc gagtccata
//
LOCUS       DAPCMT010000160         5232 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPCMT010000160 DAPCMT010000000
VERSION     DAPCMT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.163"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            170..703
                     /locus_tag="RD743_002528"
     CDS             170..703
                     /locus_tag="RD743_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564311.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            715..918
                     /locus_tag="RD743_002529"
     CDS             715..918
                     /locus_tag="RD743_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564312.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            1240..1620
                     /locus_tag="RD743_002530"
     CDS             1240..1620
                     /locus_tag="RD743_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDU0564313.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            1602..2516
                     /locus_tag="RD743_002531"
     CDS             1602..2516
                     /locus_tag="RD743_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDU0564314.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            2761..3450
                     /locus_tag="RD743_002532"
     CDS             2761..3450
                     /locus_tag="RD743_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564315.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(3571..3657)
                     /locus_tag="RD743_002533"
     CDS             complement(3571..3657)
                     /locus_tag="RD743_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDU0564316.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            4333..>5232
                     /locus_tag="RD743_002534"
     CDS             4333..>5232
                     /locus_tag="RD743_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDU0564317.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALS"
BASE COUNT         1882 a          918 c          989 g         1443 t
ORIGIN      
        1 acaagaattt aatataacat attttcctaa tgcattctaa ctataccaca ttatcaaaaa
       61 tataaattat ctttactaaa taaaatattg acaagcctat accttttgtt ttatgatttt
      121 cacaaagaaa acgttttcta tatatttttc tgtatcagga ggaaattcta tgaaaaaatt
      181 tactttaggg gtagctttgt taactattgg ggcagttgtt ttaccgtcaa ctactatagt
      241 ttatgcttca gaaaattccc aagtggtatc attaaacgaa aacaatgcta ctgaaaatgt
      301 agttattgag tacactgaag atggtatggt cgtaactaaa tatgaggatg gttatagtgc
      361 ggaagatttt gcaaattctt cagatatcat gacaagagct gcagttgcaa gatggggcaa
      421 aaaggaatat acaaatattg ctgtaaccac tggagtagct gctaatgcta ttaatgctgc
      481 cttttgggca ggagggaaag ctagcacttc tatgtttggt atcccaggtt gggcaattgg
      541 tgctttgtta acaggtgcaa gttggactaa aaaaggtgct tctccaggga atgcagttgc
      601 aaaagaatgg gacaaaaatg gtaatggctg gattggattt tattaccaaa ggggatatga
      661 tgcagctgga agagttgtag ctactagata ttcaacgtta taagttaagg agtgatgttt
      721 ttgatatcta aatcattaaa atcattaaaa acatttacta aaaagaatcc gcaaatagct
      781 actattcgta tttttatatt tgctatagct gttgctataa ttgttaatat gctaagcgga
      841 gggcaattat acaaatctgg tttattttta gtgttaatct tagttgtctt agacataata
      901 aagtctaaaa aaaaataaat cgatacattt tagaagaata gggttgtttg aatattacaa
      961 agcaacaatt tcaccttcgg tgaaataagc gaaactttac aaaaagtggc tagcaaatgc
     1021 tagccttttt ttgtattgtg gagcgtggat tttgaggggg attcaagggg gagcttgcgc
     1081 tcctttgaca agcggttatt tgcaccaaat acggaggtat ttggctagca aatatagagc
     1141 ttgccaaacc cttttagtga gctttactca ttgtaccttt ttaggtacaa tggtattgcg
     1201 aacgatacgt ccgtaggacg tactgaaagg gtgggactta tgagcgaaca acatcaaaat
     1261 ttggctagcg atcattctaa aaaatatacc tatcgatccg agccaaaaca aatcagtttt
     1321 cgagtgagcg aatccgaatt tgcaaagctg aagcagtcag ctgaagcttt gcaaatgagt
     1381 gtgccggctt tcgtgaaagc caaggcacaa aacgcgcgtc tggtgacacc gaaagtggcg
     1441 ccagacattg cccaggcaat cgcgcgcgat ttagccaaag ctggagggaa tatcaatcaa
     1501 attgccaagt ggtgcaatac acaccaacac gacgttgctc ctgatgatgc tcaacgttta
     1561 tccgaaaacc taaaaatcat gcaaaaggag ctacaaaaga tatggcaaca gttaaagtaa
     1621 gtcgaaccac ttcatgtagc cgagcaatca attacgcaga accacgagca accgtcaaaa
     1681 caggaataaa ctgtgacatt gattatgcca aaagtgaaat gaaacaaata cgcacgcttt
     1741 atggaaaaga cgatcgtgta caagcacact tattgattca gtcctttcga ccaggagaaa
     1801 ttaccgctga aaaagccaat caactagggg cagagtatgc gaaaaaaata gcccctaacc
     1861 accaaatcgc gatttacaca catacagaca aagaccatat tcataatcac attgtcatta
     1921 actccgttaa tcttgaaact gggaaaaaat ttcaagccca tgggcaagca tttttaaaca
     1981 aatgttatga cctaaatgac gagatttgtc ttgaacatga cttaacaatc acggaacgag
     2041 gaaagaaaac cgaaaaacga accatgtctg aaattaaatt aaaagaaaaa aatgaaccgg
     2101 tttggaaaga cgaaattcgg tttgcgattg atcaaacaat gaaaaacaaa aaaactcgaa
     2161 cgtataccca attttgtgat tcacttaaaa cattcggcat tcattgcttg aatcgcggaa
     2221 aaaattttac ttacgaattg gtccacaaaa agaaaaaagt acgatccaac aaactaggaa
     2281 aggattacga aaaggagacg attttacatg agctggacag acgagaacga acctacaatc
     2341 aacaacgaac caacgagcta cgaagagctc gagctagcat tcgcgaaact gcagaagaag
     2401 tacgaagaga acaacgaact tttacgcctg aacaatcgga aattagaccg actcgaagaa
     2461 accatgaaca acacgttgaa cgaggaacag accaccaaca agaattatcg ctctaaaacg
     2521 ttaaaagaca tcgaaaacga aataaagaga caacgtgcct ttcttgcgcc ttatacccaa
     2581 gaaaggatac aaactacgat ggattcacaa gtcaaaaaac tccaccaagc gctattagac
     2641 cagcgaaacc aagaacaaaa aaaggcttta attcaacgat cgatggatat tcttgccccc
     2701 ttatgttgct tagtactaac attattagcc ttttggttgg tatggggaga ataaacagac
     2761 atggcattct atttacccta tctactcatt tctgccagta ttttaggaat tatttggctt
     2821 agctataagg cttatcaaac aagatattca ttaaaaggct ctaaaattcg atttaagcga
     2881 tttttattgg tcggggttat atttgcctta tcaatcgttt taagtggttt attcggcgtt
     2941 ttagagggta ataaacgtgt ttcaagaagc attttattag gggatatcac tcaaaagtat
     3001 gaagcagcat gtgataaaaa gaaaaaagag caagcattag cacaaaaaat ggaggaattt
     3061 accgcttgtt atgaggacat gaacaatatt tttgtggatc aagagaaacg tttaacagat
     3121 aaaaacatga aaaaactcac tcgtttttat caaaacttac cagaaaaaca gcaaaaagag
     3181 gtacaagaaa aatatgagca agtaaaaaaa gacgtgcaat attttaaaga tacgcagaca
     3241 gaagaagctt gttgtaactt atttagcgat acaatccctt tttcaaccag tgaacaagaa
     3301 cgaaaagaac ggcaacaatc ggtcacatac gaacgttatg aaacactgct tcagcaagca
     3361 acaaacatac aaaatccaac gaaaaaagaa acggcattaa attatctaga aagtgtcaag
     3421 gaatggttgg atcagcaaca acaaaattga atttgattag caatcatgtt aaactgaacc
     3481 tgcaaatgat caaacattag atacacgaaa gccccagaga ggtcggattc tctggggctt
     3541 tttttcaggt tgatgtcgcc tttattgatt ttacttcttg tttcggttat tcaaccaata
     3601 actgaataaa gagacacaaa cttctacaag aagggggcaa atgaaccgtt caaacattag
     3661 atacacctcc ttccagtcgc aagactgtcg gataggcgac aagttattat accttacaaa
     3721 ataaaatctg tgtggtgttt ttaagtatcc cttattttcg ggttgttagg gcttgccctg
     3781 accgcctgta aggcgcttta agaacaagat aaggtttttt acccaagact gtcttaaaac
     3841 gacttagacg agctatcaga ggcttccccc acctccgtta ctgacttgtt tttagcaaag
     3901 gagcctttag gcgactgcgc agtacaatgt gagagcatag cgatcacact tttttagcga
     3961 ccgtagggag caaggttttg aattggcagt tttgaaaaat gcgcgaacct gttggtagaa
     4021 aaaagaagtg gtttttaggc gggaaatttt gtcaccctct tttataatct ctttttaaac
     4081 ctttttttaa acctctttac cgtcggctgt gtccttactc cttcaatgga tacagcgttt
     4141 tataaactac attttgtctg cttttaaact acattttgtc tgcttttaaa ctacattttg
     4201 tctgctttta aactacattt tgtctgcttt taaactcttt ttttgcactc acgtatgtta
     4261 taatatgcat tatcacagac aaaacgttgt ttataacgtc tgtatccatt gaaggagtaa
     4321 ggaggaatat ctttggcaaa tgaaatcgtg aaataccatc atgaattaaa taccattccg
     4381 ttgcggaaat ttacaccagt tgaaatgaat ctatttttct ctattgtgtc tagaatgcgc
     4441 gaaaaaggcg ataaaacagt ccgtcttact tttgaccaac taaaagattt aagtaattat
     4501 aaagcaactg ctaataatcg tttcattaat gatttagaaa tgacttatga taaattaatg
     4561 gatttacgtt ttggtaaaag aagtgctaat gggctcgata tagatagatt cgttatgttt
     4621 agtgagttta aaatacgtgg aactgctaaa gatccatatg tagacataca agtttacgaa
     4681 aaagctttac ctttactaaa caatttagat gagtgggttc gctattcact ccaacaattt
     4741 aacgaattac aaagtagcta tgcaaagact atgtttcgat tactaaaaca atatcgaact
     4801 aaaggttttg cttatttttc taaagaggat tttcatgaac tattagatat tccaaaaagt
     4861 tactggaaca accaagcaaa cgttgataat cgtgttataa agcctatacg acaggaatta
     4921 accgcaatct tcaaaggatt atcaatcaaa aagaaatacg gaaaagggcg aggaaaacct
     4981 gtcgttggct atcaatttac gtttaaacca gaaatcaaaa aagcagatga ttttaataaa
     5041 catgcaccaa ccaaagaaga acaaaataaa gcattggctt tagctgataa acaaaaagaa
     5101 tacgaagaac ctgctacaac aattaacgaa aaaatcaaac agaagaaacg agaacttgaa
     5161 ctcctccaac aaatcaaaca actagaaaaa gagaaagaag ctcttgaaaa acaaacagag
     5221 tccgcacttt cg
//
LOCUS       DAPCMT010000161         5198 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPCMT010000161 DAPCMT010000000
VERSION     DAPCMT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.164"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            24..695
                     /locus_tag="RD743_002535"
     CDS             24..695
                     /locus_tag="RD743_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDU0564318.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            754..1194
                     /locus_tag="RD743_002536"
     CDS             754..1194
                     /locus_tag="RD743_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564319.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1206..1874
                     /locus_tag="RD743_002537"
     CDS             1206..1874
                     /locus_tag="RD743_002537"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDU0564320.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1938..2561
                     /locus_tag="RD743_002538"
     CDS             1938..2561
                     /locus_tag="RD743_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDU0564321.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2617..3003
                     /locus_tag="RD743_002539"
     CDS             2617..3003
                     /locus_tag="RD743_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDU0564322.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3216..4607
                     /locus_tag="RD743_002540"
     CDS             3216..4607
                     /locus_tag="RD743_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDU0564323.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1727 a          861 c         1095 g         1515 t
ORIGIN      
        1 atgatgaaaa aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg
       61 agcgatctac atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac
      121 cagagttcca tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg
      181 ctgttttgca cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt
      241 ctcttgaatc ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata
      301 caaacgatgg gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa
      361 tagatgacga aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt
      421 taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc
      481 caggtctgct tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta
      541 tacgtatgca acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag
      601 tagtcaacag agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg
      661 tccttcacca agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg
      721 tcataataaa tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta
      781 atacgatttc agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag
      841 ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc
      901 tatagaatcg gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta
      961 tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga
     1021 gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt
     1081 tatacttttt ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga
     1141 cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga
     1201 aaagaatgaa attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact
     1261 ggacaggatg gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg
     1321 gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac
     1381 gtgcgattct taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa
     1441 taaaaacatg gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata
     1501 agatgaaaga aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag
     1561 ttccgccgtt aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt
     1621 tgatcccata cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag
     1681 atcatctagc accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata
     1741 gcatccgagc actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg
     1801 atatcccaaa tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca
     1861 aaaaaatact ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg
     1921 aatggaacaa aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata
     1981 cgtatcccgc aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg
     2041 acaaaaaaga aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat
     2101 caaacttgaa tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg
     2161 aagtttcccg tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg
     2221 gtcgtcattt catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac
     2281 atcgattggc acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac
     2341 ctgatccagc atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga
     2401 tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt
     2461 gtctttacga catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc
     2521 tgaatgaaat ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga
     2581 tatgttataa ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa
     2641 gttgtagaag catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg
     2701 aaagtaggct tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc
     2761 gtaggagcac gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg
     2821 agtcagatca atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa
     2881 gaagtggacg agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg
     2941 acagagatcg ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa
     3001 tagacagtaa aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac
     3061 taattgtaag caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg
     3121 aaaaaaattt tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg
     3181 atggacttgt cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag
     3241 tggttctagt ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa
     3301 aattttcaac acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc
     3361 aattgatctt tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg
     3421 taggtcaagg atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga
     3481 tcattggtgc attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg
     3541 tattgacttc tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa
     3601 tgctgttcgg actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct
     3661 ctgaactggc aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat
     3721 cctttatccc taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg
     3781 gagtcgttat tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc
     3841 caaaagaagc agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga
     3901 tcgtgacgct tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt
     3961 tggcaacaag tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg
     4021 tagcattgct tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc
     4081 caatcaacta tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa
     4141 gtgctggagc tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg
     4201 caacagctaa tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg
     4261 tttatccagc tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt
     4321 tggaatttat tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag
     4381 tcggtggggg cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag
     4441 caatcgttgg gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa
     4501 acgtcagcgg tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc
     4561 gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt
     4621 gtgatagaag tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa
     4681 tttttgtgaa atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg
     4741 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4801 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gtttaagaaa
     4861 gaggtcgtga gcctgccgtt tcactctgag tcacaaagag caatttcaaa aaatagcttc
     4921 tcatgtttgt tgaatattgt gtcttgtgac cgaggagttg tcaggagaac accgtttacc
     4981 agaggttgtg acttgttttt agtcacgacc tcttttttta tttattccat ataaatacac
     5041 attatttatt ccgaaaagtg aaagcgcaaa taacaaaaac aaccaactta tttattatta
     5101 aagaaaagcg tatttaagta ggacgattct gttgagagct ttcgtagatt ttcccatttt
     5161 cctatggtaa actgacaaag ttgagaatgt tagaacag
//
LOCUS       DAPCMT010000162         5179 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPCMT010000162 DAPCMT010000000
VERSION     DAPCMT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.165"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(13..1440)
                     /gene="uxaC"
                     /locus_tag="RD743_002541"
     CDS             complement(13..1440)
                     /gene="uxaC"
                     /locus_tag="RD743_002541"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDU0564324.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(1514..2593)
                     /gene="uxuA"
                     /locus_tag="RD743_002542"
     CDS             complement(1514..2593)
                     /gene="uxuA"
                     /locus_tag="RD743_002542"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDU0564325.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(2596..4227)
                     /locus_tag="RD743_002543"
     CDS             complement(2596..4227)
                     /locus_tag="RD743_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDU0564326.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            4550..>5179
                     /locus_tag="RD743_002544"
     CDS             4550..>5179
                     /locus_tag="RD743_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0564327.1"
                     /translation="MKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQKK
                     QVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERFRW
                     LRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVV"
BASE COUNT         1638 a         1027 c          743 g         1771 t
ORIGIN      
        1 ttattttttg tattattttc tactttcgat ttcaaaaaaa ttaaaataat tatacgcatt
       61 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcgacc
      121 tgctttaacc cactcgctta gtaattgaca taatactcga cggaaatact catggcgagg
      181 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
      241 ttgcgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
      301 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
      361 tggaagccaa tcggttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
      421 ttcagcagtg tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
      481 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
      541 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
      601 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
      661 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
      721 accgtgatct gctaatcgac agccaatttg atgaaaataa tccactcgtt ggctcaatgc
      781 ctgcttcaac ccattaaagt ttttgattgg tatacccgct gcttgactaa gttttaaaat
      841 atagttcgaa aatcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
      901 taccttgaaa gaattatcct gtttagaaag ctcagtatga tatcgcaagt catctgtagg
      961 atcatccgtt gtacaaatgg ctttcacatc catcatcttc agcaattctt ttgggcgata
     1021 ggaatccgtt tggatttttt cgttggcacg tcgccaaatt tctggtgcat tcttctcaga
     1081 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
     1141 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcattacc
     1201 tgcaccactg atcaactctt cttctatccc attagctcgc atcaatcgcc atttataatg
     1261 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
     1321 tagcaaacat gctgggtcta aatgacaatg ataatcgatg attggttggt gttctgcaaa
     1381 tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat
     1441 atttaagcac tcctttcctt cccttagttt ttagaagaga aaatttttct tctaaataga
     1501 ctccattata taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat
     1561 aaactgcacc tagagctcgg tcatataacc cataaccagg tctaccggtt tctccccaaa
     1621 tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta
     1681 atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttca taaaaatcga
     1741 gtccattatc acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt
     1801 gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag
     1861 tacataacgt aataccatta gaaggagaat ccactacatt acacaaccat tctaaatcgt
     1921 cacgattttt aacaatacga ggtaatccga aaatagaata tggtggatca tctggatgaa
     1981 ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa
     2041 agttctctcg caatttcgct tcatcaactt ctttataggc ttctaataat tgcttgattt
     2101 gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt
     2161 ttctaataat ttcctctgga tcagtaggaa tattttttga aatataggct aacgtagaag
     2221 acccatcatc taaatgataa tccaaatctg attttaccca atcaaagact ggcataaaat
     2281 tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct
     2341 cgatgtattg atctcgtgtt ggcaaaccaa ttttgatatc atcatgaaca ttaacactct
     2401 caataacttt caaggtaagg ccattggctt cgacttcatc ttttaaagct ttaattcgtt
     2461 ctctcggcca gacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg
     2521 ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa
     2581 aagtcatttc catagttact ttcctccaac ttctgttata tatttattga ttgttttacg
     2641 aactgcttga ggacctacta gcatttcgca aaagattttt tcaattttct ttcctatctc
     2701 aacttgatat aaatctaaac caaatatttc cttattttct aagatttcct gtaccgcaca
     2761 atgaatcttt gcaggatctt gttcacctaa tttcaatact gagaattttt cctgcaactc
     2821 taccaataaa ggatcaggac ttggcacaaa attttttcct tgatcatcga ctgccaataa
     2881 ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc
     2941 tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc
     3001 tgttgcaatt cgttgcggtg tatctggaat atagggatta ggcaaacgtt tattgattac
     3061 ttcatcaata aattgttttg gatcaataac gcctggactt tcaactactg gtaagccttc
     3121 tacataacct atccctttaa ttaagccaac caaatcatca tcttgcattt ctgcctgaat
     3181 tgatttatac cccaacaaac aacctgttat tgctaatgcc gtatggagcg gatttaaaca
     3241 tgtcgtaacc ttcatgatat ctgctttatc aactgtttct ctatctgtca taatcacacc
     3301 agccgcttcc agtttaggtc gaccatttgg aaaactatct tcaatgacta gataatggac
     3361 tttttcagta ttggtaaaag cagcgatatt ggtatttttc gcagtatgaa ttatatctaa
     3421 gtcttcgaaa cccgccttca ataaatccat tttgacactt tccgaaggat ttggcgtaat
     3481 tctatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa
     3541 tttttcagaa acgtatcctt ttttttgcca agcttctgca atcataaaaa tgctatcttg
     3601 aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg gtaactctcc
     3661 agcattaaac ctgctataca acaaggatgt cacaatactc atcgtatgtt gaggattggt
     3721 aggtccagat tttatatctg cttccactac cgaaaaaaat tgtcctttag aatcttttaa
     3781 actgtatcct ttttcagtaa tagtaaacgt tgtaaactgt aatgtaggat cttcaaaata
     3841 tttaaccact ctttgatagt ccgttatatt ctttggattg caatataaac tatcagctgt
     3901 tactgctaaa agtctttttt tgagttcacc atcttcaccc ataacaatct ccaaaatatc
     3961 attgttgtag tcatgataag ctttttctac tacttcttca tcaaatgtct cacacacaac
     4021 tactccggat tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
     4081 tcgataaaga tttcctccac caaaatgaag ccacactgga tgtttctgac cttcttgttt
     4141 gaccgcttcg acattaaaat acggtagttg aacttgcatt ttgttcaatc cttcgataga
     4201 atcttgccag tctttaatca aaaccatatt tacccctcca tactgatatg ttagtttccg
     4261 ttacaattgt agtataaaat cgaatgtaat cgctgtcaat aagaaaaaac taaaaatatt
     4321 cttttttcta ccatgtcaga attattgtgt ctttttttag taaaagcaca ccaatatata
     4381 ttttttagaa ctaacttaaa ttaaagctta atttctcttt gatcttattt gagtattttt
     4441 tgttttttta atagctagta tgttactttg aaatttgata ttatataacg agcatagaac
     4501 taaaatatca aattcaaaaa tgaaattttg agagggatta aatatgacaa ttaaaactag
     4561 tctccaaaat caagcttatc aatctattcg tcagcaaatt atctacgctg acttagaacc
     4621 tggaaaaaaa gtatcagaaa aaaaattgga agaaacgctt tctatcggtc gtactccaat
     4681 acgtgaagca ttgattcaac tccgtcatca aggacttgtt gatacaattc ctcaaagtgg
     4741 aaattatatc tcattaatcg acttagattt agcaaaaaat gctcgttttg tcagagaaca
     4801 tttagaacga caaattatgc tagagtgttg tgccaagatg aataatcaaa agaaacaagt
     4861 tttagaaact ataattgcag aacaagaaaa agctgttagt caaaagaatg ctcgtagttt
     4921 ttttcataat gataatttat ttcataaagc ttgttttgaa attgcagaac gggaagaagt
     4981 atggcaatgg ttggaagata ataatacgca cttggaacga tttcgttggt tacgagtaaa
     5041 aacggaagga ttaaaatggg aaaaaattat ggaacaacat catcaactat ttaaagcctt
     5101 gaccatgcaa gaccctgaag aagctaactt cttaacatcc ttgcatcttc atttaatgct
     5161 tgatgaacaa aatgttgta
//
LOCUS       DAPCMT010000163         5007 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPCMT010000163 DAPCMT010000000
VERSION     DAPCMT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.166"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..182)
                     /locus_tag="RD743_002545"
     CDS             complement(<1..182)
                     /locus_tag="RD743_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDU0564328.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="RD743_002546"
     CDS             517..951
                     /locus_tag="RD743_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDU0564329.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="RD743_002547"
     CDS             974..1294
                     /locus_tag="RD743_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564330.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="RD743_002548"
     CDS             1435..1626
                     /locus_tag="RD743_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564331.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="RD743_002549"
     CDS             complement(1697..1954)
                     /locus_tag="RD743_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDU0564332.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="RD743_002550"
     CDS             2112..2375
                     /locus_tag="RD743_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564333.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="RD743_002551"
     CDS             2399..2587
                     /locus_tag="RD743_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564334.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="RD743_002552"
     CDS             complement(2670..2855)
                     /locus_tag="RD743_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564335.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="RD743_002553"
     CDS             3070..3441
                     /locus_tag="RD743_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDU0564336.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="RD743_002554"
     tRNA            complement(3591..3663)
                     /locus_tag="RD743_002554"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="RD743_002555"
     CDS             complement(3709..4137)
                     /locus_tag="RD743_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564337.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="RD743_002556"
     CDS             complement(4197..4955)
                     /locus_tag="RD743_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDU0564338.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1744 a          784 c          978 g         1501 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt attttta
//
LOCUS       DAPCMT010000164         4980 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPCMT010000164 DAPCMT010000000
VERSION     DAPCMT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.167"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            500..1411
                     /locus_tag="RD743_002557"
     CDS             500..1411
                     /locus_tag="RD743_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HDU0564339.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1898..2164
                     /locus_tag="RD743_002558"
     CDS             1898..2164
                     /locus_tag="RD743_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564340.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2236..2577
                     /locus_tag="RD743_002559"
     CDS             2236..2577
                     /locus_tag="RD743_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564341.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2632..3051
                     /gene="ssb"
                     /locus_tag="RD743_002560"
     CDS             2632..3051
                     /gene="ssb"
                     /locus_tag="RD743_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDU0564342.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3128..3424
                     /locus_tag="RD743_002561"
     CDS             3128..3424
                     /locus_tag="RD743_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564343.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3439..3870
                     /locus_tag="RD743_002562"
     CDS             3439..3870
                     /locus_tag="RD743_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDU0564344.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4052..4357
                     /locus_tag="RD743_002563"
     CDS             4052..4357
                     /locus_tag="RD743_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564345.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            4761..>4980
                     /locus_tag="RD743_002564"
     CDS             4761..>4980
                     /locus_tag="RD743_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDU0564346.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDS"
BASE COUNT         1845 a          743 c         1034 g         1358 t
ORIGIN      
        1 acataccaag cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat
       61 aaaatttaaa agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc
      121 cccctaaccc cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac
      181 gccccagtgt tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa
      241 gtgtagagga aacttgaaga cttgttcgaa cagaaacttg cgagagagcc ggagtaaaac
      301 aagtgtagag gaaacttgaa gacttgttcg aacagaaact tgcgagagag ttggagtaaa
      361 acaagtgtag aggaaacttg aagacttgtt cgaacacaaa cttacaagag aggggagtaa
      421 agcagttgca gaggaatcag gaagatttgt tcgaacagaa acttgcaaga aagcattatt
      481 aaattgaagg tgatgaaata tgaagtattt attgcatggc tattttattg attattctac
      541 tcgcaatagt agtactttag cgattctatg tttgcttcat gaattgagag tggttactag
      601 aaaacagcta tacaaactat tgaattttga cagatcaatt agtaaacaag gagtaacgaa
      661 tgctttagtg agattatatg aaaaacagtt aatcgatcga aaaaaatatg gaacagaaat
      721 gtattattat ttgacgaaag agggacatca aagcataggt gggtattata cgttgccgaa
      781 agttcctgaa tataatttga atcaccattt acaaattaac aattatttga ttaaaggaat
      841 ggcattggca aaagaccacc cacatttaaa acggattatt tcagaaagac gtaccgtcta
      901 tgaaacgaag gatttaaatc ctaagacaaa aggaagaaaa ttttttgtgc cagattttat
      961 ttttcgtttt cgagacaaag aaaatcatga gatcaattgg tcctttgaaa ttgagctaac
     1021 gttgaaaaca cgtcgtcgct accgtgaagc gatttttccg aaatatattc gtgagttgcg
     1081 aacacgtcct gaatcacatt taatttatgt gacacctagt ggcgcaattc aagaagaact
     1141 tcatgcgttt aagcgtattt ttttcaagcg cgaaactgaa aaagatgcga gtcaacaaga
     1201 attgtttgag cggttgcata ttttttcagc tttagagttt gaagagggat taaaagcatt
     1261 attagagaat gatccagcag tcaattggcg gacagtagaa cgagaaaaaa tttatccgaa
     1321 tggcgctgtt caaagtcgtt tgaatttatt tgaagaatca actgaaaatg aggaaaaacc
     1381 aaaagtgaag tttacgcctg aagtattgta aaagagggtg ttcgcttcgc tctttttttg
     1441 tgccagtttg ctggcaccgt tcagacgcaa gcgtctgaac cggctcgttt ttaggaagga
     1501 ctaacttgtt tgatttatcc gttgtcaaag aagtggacgt tcgagagtcc ctaatcgtat
     1561 agcagtcatt cggcgagtat aacctaaaac ataacctgcc aattgccaag ggtaaaatgc
     1621 acgctgcgcg cccttggcaa tcgtcagaac atgttttcga gccggttatg ccgaacgacc
     1681 gctatacgaa aagggcctct aaacagaagt ccactttgat ccttgacaac tgaaacccaa
     1741 aatcaaacaa gcaggccttc gtctaaaaac gagccgaatg aagaccttgg gggttgcacc
     1801 cccaaacccc cgtgcccccg acacactacc gaaaaggtgg gggtgggtgg cgaaaaaatc
     1861 aaataaaaca ttctattcaa gaaaggaaga aacaaccatg acaactaaaa acgagcaact
     1921 gaaagaacag gcaagaaaag agttgcaaga aagaggtttg attattgagg ggagctttga
     1981 aggagatttt gaaacctata ttggttgtta tgcaagaccg ataaacaaac ctacagcctt
     2041 agacccaatg aacgaacaag aagtaaaaga acaagaaaaa catgcagtga atggttttcc
     2101 gcaagatttc actgaatggt atgagtggga aattaacaac gggaagcttg aaaacttctt
     2161 ataaaagtca gatgacgtgc aaagagaatg attctctttg tacggtaaaa atataaagaa
     2221 agaaggaaaa agaaaatggc aaaaaacaca ccttatcaag taggagacat tttttatagt
     2281 agttggggct acgaacaaac gaacgtgact ttttggcaaa ttgtgaagtt aacagaaaaa
     2341 acagcatggt ttcagcctat tcaaaagaaa acggtcgagc attattcgtc catgagtgga
     2401 aaagttgttc caatgaaaga caagtttata gagtatcctt tagcaaaaac taaagacagt
     2461 attgtaaaaa aacgaattga tccagaaacc tatcctgata tgttatgtgg ttatcgtagt
     2521 tgggacacgt ttcatagata taaaaatcaa gctgtttatg aaagtagtta ttattaaggg
     2581 gatagtttcc cctttcaaaa ataataaata gaagaatgga gagaaaaaat catgatgaat
     2641 tcagtgaatt tagtaggacg gttaacaaaa gaagtggatt taaaatacac accaaaaggc
     2701 aatgcgacag gcacatttat tttagctgtt aatcgaaatt atacgaatgc aaacggcgag
     2761 cgtgaagcag attatatccg ctgtgtcatt tggcggaaag ctgcggaaac tttagcaaaa
     2821 tatactggaa aaggaagttt gattggcatt aatggacgtc ttcaaacgag gagctatcag
     2881 aatcagcaag gacagaccat gtatgttacg gaagttctag tgacagattt ctatttgtta
     2941 gagagtaaag aaatcaatga gcaacggtca aaatcaatgg cgaataatca aaataattct
     3001 tcaattccag gaaatgagat tcaaatttca gatagttatt tgccctttta agtgaaaaat
     3061 aattttgttc tatacctgat ttcttcaggt atagaacaaa aaataaatag agatgaggcg
     3121 attttttatg aaaatcattg acagttacga tcaacgaacc atgcaatatt tgggctcgtt
     3181 tgattataca gatgaaaata ttattaatta tgttgcaagt ttacctcctt atcaaagcgt
     3241 tcgcttggta gattttgcaa gtgatgaatt ggtattaaca acgatgggca actttttaga
     3301 ttatgtcgga gacaaaaaat ggttggaaaa aattttgtct aaattgattg cgaaacaaac
     3361 aggagaagaa cccataaatg cgattcaatt gttcaatcga tatgaacaag aaaacaatga
     3421 ttaaagagga gagaaaaaat gaaaagatta aaacgaagta tttttattgt gctagcattg
     3481 attgattttg ttgggacagg ttgtgaagca attgacgttc aaaaagctcc cttatcggaa
     3541 ataaaacaag caataacaaa acaagatgaa gaacaaccgc taaatacaca agtagaaata
     3601 gtgaatcaaa ataaaccgag tttttctgaa gaagattttt cgttagaaaa tggaaccagg
     3661 caattttttt cagatttgga tcgtttaaat cgggtaggtg tagcaagtgc acttttgcat
     3721 aaaagcagga tgccaacaga agaacgagac cgattataca ttaagccaac tggctggaaa
     3781 caaaagaaaa tgaagaatgg cgattatctt tataatcgtt gtcatttgat tggctatcaa
     3841 cgtgcgacaa gtttccccat aagtatgtaa tacgaaacgg gaggattaat cttaagatta
     3901 atcacaacta acctatttga cagttggaat gaaagctatc atgtctgctg aatcaactgg
     3961 tctgatactc ctacatgcaa taaagtctac taggaagtta ttgtatgaag ctaggtaaag
     4021 gcggcagaac aatttctaag tgtggaatca tatgaagttg gatatgccgg gacgctataa
     4081 attactcagg gtgagactgt ttttaattaa actgacgaac ttgcgaatgt acgggtctat
     4141 aaccgactta tattcgaaag atataagatt gcgaatgcaa agaacattat cgaaaagtaa
     4201 gaatgttggt catgaaattc gagatggaag actagcggtg tatctgtcaa gagttggtaa
     4261 aacatgtttg attgattcag atgagagtgg gaatgcgaca aaacgtttta cagaagaaat
     4321 agagcaacta gcagaactga atatacgata tttttaaata ctttagcgga gaataaaaaa
     4381 atcctctaca gaaaactacg gagggcaaca tgtgctattg tttcaacaat acatttttaa
     4441 tttttaccat aacaaaaaag tacttgaaaa aaaacagaaa tatgttaaat taaaaatgaa
     4501 aaaatggaag cggttactta ttcttttgaa tctaagataa aaattcaaaa gaataacata
     4561 acccaaatat gattatactt gagattctaa atatttatcg aaaaggaatt tgttttaagg
     4621 aaaaatgatt ctatggacgg ttattgattt atatttacca aatggctaat aaaatagtat
     4681 ttcttatatt gtatagtttt tgtttttatg tgaaaacatg agcaatagaa ttgtactagt
     4741 aggataagga tggatggaaa atgaaaaaaa ctatttttgt tttagcgatt tttatatgtt
     4801 ttgggctact tttggtaggc tacttgttgt ttaattctaa tgaaatgaga actatttcta
     4861 cgaatgactt gttaaaaaaa agtgaattaa aggataaaaa aaaagaatct cttgccttta
     4921 taatgtttac tcaagaagag tgtgagtatt gtatagcaat aaatccaatc atagatagtg
//
LOCUS       DAPCMT010000165         4979 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPCMT010000165 DAPCMT010000000
VERSION     DAPCMT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.168"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..966)
                     /locus_tag="RD743_002565"
     CDS             complement(<1..966)
                     /locus_tag="RD743_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0564347.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVS"
     gene            complement(1211..2482)
                     /locus_tag="RD743_002566"
     CDS             complement(1211..2482)
                     /locus_tag="RD743_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDU0564348.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2766..3713)
                     /locus_tag="RD743_002567"
                     /pseudo
     CDS             complement(2766..3713)
                     /locus_tag="RD743_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            3725..4198
                     /locus_tag="RD743_002568"
     CDS             3725..4198
                     /locus_tag="RD743_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0564349.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(4371..4925)
                     /locus_tag="RD743_002569"
     CDS             complement(4371..4925)
                     /locus_tag="RD743_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0564350.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         1574 a          947 c          744 g         1714 t
ORIGIN      
        1 cgaaacattt ctaacttctt ttattcgttc attaaccatg cgaactgcta gttttccaat
       61 ttcttctgta ttcacattca ccgtatttaa tgcaggtgtt aaaaattcag caacttcaat
      121 attatcaaaa ctcacaatcg aaatatcttc tggaatatgt agaccatttt tttgaagtga
      181 acgataaacc cccacagcaa taggatcatt tcctaccatg atagcagtcg gcaacttatc
      241 tttagcatgt tttttcaaaa gtaaatctgc tgcctgcatt ccgtcaagtg tggtccagcc
      301 ttctaaataa atttgactat atttttctaa tcctttttgt ttcatccacg tctcataggc
      361 tatttttcga taatcatcat ctgacacgtg tttatttcct tggctatcta aacgaacacg
      421 tcctcctccg ataaacgcaa tgtttcggtg tcctttttta tacaatcgat ctaaatgctg
      481 attcgttgct tttgttaaat cagtataaac tgcatctact tcagcttcaa tactagggtc
      541 atcaatcact acgacattcg aattaattgc tgatacttct tcaatcacag aagatagcac
      601 ctgcccaata atcaacacac cttgacattt tagcaaatcg tcttgctcca acggtgtttc
      661 tgacaaacga ataattttct tcaaattgac tttgctttgt tctgcttcga cttgaatccc
      721 tctgcgaatc gttcgaaaat agggatcttc caactcatct acttcagaaa ctgtcgttat
      781 taatgcaata tttgttaaat tttttgcaac gggacttttt ttgacataat gataaaaatt
      841 aaccgattct gcaatctttc gtttggtttc atcggttact gaaagtgtcg gatcctcatt
      901 tagcacgcgc gatactgttg caatggacac acccgcccgt tttgcaacat ccctaattcc
      961 taccataata gctcctcttt tcatatttga aagtgtttac taacatataa tcatacattt
     1021 tgaaaataag caaatttcgt taaaaatagg cactgtaatc ataattaaaa acaagaaaaa
     1081 atatccgaac aatttagagt aaataatgct aattctagtg aatttgcgac gaggaactgc
     1141 tagatcacag ttcattctct ctatcgttcg gatattttat tgttaactat acttatttca
     1201 cagagctttt ttatttgatt ttgtgaacag agtctcgaga aatcaattct ccctctaata
     1261 gtagtacttg attttttatt tcttcttgtt tggccagtcc aatcagttgg tgaaaagcaa
     1321 tcgccccctt gttacctgga tttagtcgta ttgttgtcaa cgcaggatcc attaattctg
     1381 caaaagtaat atcatcaaat ccaataatcg ataattgttc catcactcgt ttattcttat
     1441 tttttaaaaa ttttactaat ttagccgcta aataatctcc agtaacaaca attgcagtaa
     1501 attcagctaa ttcttgataa attcgctcaa attcagagtt gttatatatc caatgcgatt
     1561 taaaggttaa atgatatttc tttaatgtat cgatataagc ttgatgtcgt tcttgaataa
     1621 cacttggttc acctacaatg tagtcaaaag aaagaaacgc tattttctca tgaccataat
     1681 gaatcaaatg ttccactgcc aatgtactag cttggtaatc atttgtattt acaaataaac
     1741 gattcatttg ttgttcaagt tctcttaatg tctcttttga aagatgcgta tcaatataaa
     1801 caacaggacc atcaaaaacg ttatttaatt ccaaaaattc ttttacacgt acaccaacaa
     1861 caatgaaccc ctcaaaacgc caatttctct gaatagtttt aacatcttct gctgaattaa
     1921 cattatgcac caatacaaaa taaccatgat tctttgcctg ttcctcaatt ccagacaaaa
     1981 cttctgcggt aaatggatca gaaaatacaa aatcagcatt atcaatagcc gaaaaataaa
     2041 gcaatcctat taaaaatgca atcgtcttac cgattccctg acagacagaa cacattccac
     2101 taggatcatt aaatgaataa gcatttgcat atcctaaact aggtgttgca aaacatgaaa
     2161 acaacacacg aaataacgca ttgatgttag taatcgtccc taacgttgag cgcgcactgc
     2221 caccaatctt cttttgattg accacaatcg ccgtagaaag attatcaatc tcatcaacct
     2281 ctggacgact gtatttaggt aaaaataaac gggtaaaact atcataggtg tcattcagtt
     2341 gacgaccagc ttcttgggta atagtatcaa aaacaatcga tgattttcct gaaccagata
     2401 cgctggtaaa aatactaatt ttatttttgg gaattttgag actgacattt ttaagttgtt
     2461 tctcttgcgt ggataatttc aatatactcg ttcatttggt actccccttt attttgaaat
     2521 agttgagaat gtttcaatga gacacggtat ttttggcaga tgtttagttt acctagaaag
     2581 aggtataaaa atcaagaaaa aaagagaggc tgactcataa gtagttttat tagtcgaaaa
     2641 tgatagttta taaaaatagg aaccgcatca aatcaacatt ctttagaatg gatttgatgc
     2701 gtttttcttt attttttgac ttatgagtca acctcttact cttcgtcatg gatttgatta
     2761 gctaattatt gctccacttg ttcacgaatg aaatttaagt actgcggtaa aaataaagga
     2821 atttcttcaa agcgtggact ttgattttca tacgcttttg catctaggtt tccttcccct
     2881 gtagcaattt caatcaataa ttgatttggg tcacgataat aaagtgatac aaaaaattca
     2941 cgatctttta ttcctgaatt tataaaattg cgctcttgta attgtttgtc gatgcgttgt
     3001 aaatcagatt tattttctac gccaaacgca acatgatgga cgctaccaat cccttcaatg
     3061 gcaactggat tctcacgatc ttgaataatc tgaacttctt gatagaactg agtgtcttga
     3121 ttttctaaga ttgtcactcg ttgatgaccc gaaaagaaac tgacgtattc tttgtgctac
     3181 cagcctaccg acaataactc tcgttcagtc gcatcggaat attgtacgcg tagttgaatg
     3241 gcatcaattc ctaaaatggc atgctctgta gggatctggt ctgtgacata cgggaaataa
     3301 tcatcaatat cttcaaattc gcgtaaggga actaaagcta atcgtgttgc atctggccct
     3361 tcaaaacgta agataggtcg tccagaaaat gattcgattc cataatggca aacgccttgt
     3421 gtttcaaaat atatttccca aaaatgcaat gatgcttcgg aaggaacttt tagtatcgtt
     3481 cgttcaatcg tattggttcc aaaaaactgg ttgttccctt cttgtacttc aaaaaaagtc
     3541 agttcagttc ccacacggtg gtgattatct gaaaaaaaca ggtgatacat ggtatgatcg
     3601 tcttgattaa ttgttttcat tagtaatttt aaaccgagta tattatgata aaagtcaaat
     3661 gtcggtcgaa taaagcgatt cacaatagaa atgtgatgaa tacgtgcggg cataatatct
     3721 aaaaatgaga atcaaaccta taaaagatgg tgaacaactt ttgttttcat cagaagaaaa
     3781 taagcccctt tatttagact atgtaaatca caatttgaaa atcatcatta aagagaataa
     3841 cttaaaacat atcacacttc atggttttcg tcatacccat tgtagtctgc tctttgaatc
     3901 tggtgcgtct gtcaaagaag tgtatgttcg acttggacat acagatatta aaaccactat
     3961 ggatgatata tacacccata tcgcgaaaaa gcagacagag gaaacggcaa ttcgttttgc
     4021 agatttcatg agttctacag aggaaaagca aggaagtttg cagaaggtca aaagtatggt
     4081 caaaaacgaa aaaagcagac tatccctatt agatagtctg ctaaacgttg agttatcagt
     4141 atttttctta ttccgcagcg tttgcgtttt tgagaccgta ttgattctct ttttttaatt
     4201 tcatcctttt ccaacctaaa aatgttattt aattacttag tttatataac acgaacctat
     4261 accaagtacc agtaacccca gcttttattc cgcgaacttt atgaaataaa atcgttctag
     4321 ataaaaatag cttttaatct ttgtctttac tgtttataaa aatttttaaa ttatacacct
     4381 agtttaaatc cacgaagtga tgctcctatt tcatcttcag ttataccaat gtaccttttt
     4441 gtaattgctt gcgagctatg attaaaaata aacattaagg tagcaatgtc tctagttttc
     4501 ttgtagtagt gatacccaaa agtttttctt aatgtatgtg ttcctatatc atctcgtcct
     4561 aatttttctg ctatatctgc atatatctga tagacacgat cccttgttat atgtcgacct
     4621 tgttgccgac tcggaaataa ccaatcattt tcttctttac cttcagtata taataaaatt
     4681 tcttctctca aattatctat aaaaatcggt tttcgcttac ctgttttatg ttcaacaata
     4741 ataggcttac ttttgcctcg tatatctcca acttttaatc ctataatatc tgacatacgt
     4801 aacccattat tgattccaaa caaaaataaa aaactatctc tttccggatt acttgttctt
     4861 cttagccaaa actgaaaatc ttgtatctct tcatttgtcc ttaacggttg tacattatag
     4921 ctcactattg tttcccttct taaaaatatt ctgagttaat gattatgtat atattttac
//
LOCUS       DAPCMT010000166         4918 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPCMT010000166 DAPCMT010000000
VERSION     DAPCMT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.169"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(145..1755)
                     /locus_tag="RD743_002570"
     CDS             complement(145..1755)
                     /locus_tag="RD743_002570"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDU0564351.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2948..4288)
                     /gene="glnA"
                     /locus_tag="RD743_002571"
     CDS             complement(2948..4288)
                     /gene="glnA"
                     /locus_tag="RD743_002571"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDU0564352.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4316..4708)
                     /locus_tag="RD743_002572"
     CDS             complement(4316..4708)
                     /locus_tag="RD743_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDU0564353.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1450 a         1058 c          802 g         1608 t
ORIGIN      
        1 tccaatagcg ctttaatcga aaaaagtctt ttgctttcta tcttttttcc tacaaataat
       61 aacaaatcag aaaaagagaa aagtgacaag aaaattgctt atccccttct cttgctactt
      121 ttctctttct atcaagttca aaaattattt tgtttcgtga tctgctaatg atgctccgac
      181 aaatgctttg attaattttt gcgggcgatt tggacgagaa atcaattctg ggtggaattg
      241 gcatcccaca aagaattttt tctctggcag ctctacgatt tctactaaac ggttatctgg
      301 tgagacacct gaaaagacta gcccttgttc ttcaaacaat tgacgatatt tgttattgaa
      361 ctcatagcgg tgacggtgac gttcttgcac aacatcggct ccatcatatg cttcagccgt
      421 cttcgttcct tttttgattt tacatgggta taatccaagg cgtaaagttc cacccaaatt
      481 ctcgatgttt tcctgatcag ccattaaatc aataatattg tttgctgtat ctggattcgt
      541 ttcagctgat gacgcatctt ctaatccaac gacgttacgt gcaaattcga cacaagccat
      601 ctgcatacca agacaaatac ctaaaaacgg aacgtcattt tcgcgagcat aacgaatcgc
      661 ttcgattttc ccttctactc cgcgatcacc aaatccacca ggaaccaaga ttccatctgc
      721 gtctttcaaa cgttcttcta cattttctct cgttaattct tgtgctttga tccaatcgat
      781 ctcgatgtct gaattatagg cgaaaccaga atgtttcaat gcttcaacta ctgagatata
      841 tgcatctggt aactcgacat atttaccgac aagcgcaatc cgtacttttt tcttcaagtt
      901 caagacacgt tcttctaatt ctttccattc tgtcatgtct gcttctggtg tatctagttt
      961 taaatggtcg caaacaattt gatccattcc ttgtgcttgt aaaactaatg ggatggagta
     1021 aagtgtatct acatctggcg actcgataac tgcttctggt tcgacatcac aaaattgtgc
     1081 caatttgttt ttcacgcttt gagaaacagg ttgctctgta cgtaccacca aaatattcgg
     1141 ttggatacct aaaccacgta gctcttttac actgtgctgc gttggtttag ttttcatttc
     1201 tccagcagct ttcaagtaag gaatcaatgt tgtatggata tacataacat tatctgtacc
     1261 gacatctgct ttcatttgac gaagtgcttc aaggaatggc aaagattcta tatcccctac
     1321 tgttccccct acttctgtaa tgatcacatc tgaatcagtc attttagctg ctcgcatgat
     1381 tttttctttg atctcatttg taatatgagg aataacttga acagttgctc ccaagtattc
     1441 gcccttacgt tctttacgca aaacttcaga atagattttt cctgtcgtca cgttggagta
     1501 tttgttcaaa ttgatgtcaa taaaacgttc gtagtggccc agatccaaat ctgtttcagc
     1561 accatcatct gttacgaata cttctccgtg ctgatatggg ctcatcgtcc ccggatccac
     1621 gttgatgtat ggatcaaatt tttggatagt tacttttaag ccgcggtttt tcaataagcg
     1681 tcctaaagaa gctgcaacaa tcccttttcc gattgatgaa acgacaccgc cggtcacaaa
     1741 aatatatttt gtcataagtt caaaaactcc tttttaattt atgtgcagga aaagtctcag
     1801 acaataaaca aagctcccta ttcttcaata agaataggga gccgaaattt ctgtaaactt
     1861 ctgaccctct aaaagggtgc ccaaggtgta taatacaagc tgactaaggg aaagtcaagt
     1921 actcaaatga atactatccg cttacaattt tttctttttc gatatccttt gagaaattat
     1981 ttcttaagtc ctttatgagt gcaaaaaaaa tttaacccac ataataagct tgatataaac
     2041 atagctaacg ttaaacctaa gcaaatcaga aaatcgttac tgaaaaattt taagtgaaat
     2101 aatagataaa aaaataaaac aaacattatg atagataata tcccacctaa caaagaacta
     2161 ctttttccta cttgcagcaa cagtttatat agcttatcgt agaaacaatc tttttctttc
     2221 acccttattt acctccttta cgtaaaaaac gtagtccaca gttctcggat ttttggcata
     2281 tcttttctat taaataatat caaaaacaaa acaattccac taattttttc aaagatgacc
     2341 tttttttcaa caaaagtgcc ttgtagggat tcattgaagg taacgaatca cataagtaaa
     2401 aaataaggag atgcgacaaa agtcgtgcct ccttatttct aaataaacgg tgttctcctg
     2461 ccaactcctc ggtcacaagt cacaatatcc atcaaacatg agaagctgtt tttgaaatta
     2521 tttcttgtga ttcggagtta aatggtaggc tcacaacctc tggataaacg gtattcaaaa
     2581 gccagtttct cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa
     2641 tttttcttaa tactcgaaac taaacgtctt tttcaaaacc tcttggtaaa cggtgttcaa
     2701 aagcttgcat ctgcggtaat aagttcagcc aattcaaaaa caagaaaagc tgtttttgct
     2761 tggctattct tattacttgt tgcagggcag ctttttcaca acctctccac taaacggtgt
     2821 tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga actatttttc
     2881 cgttagtttc tctaatactc aggtcaaaac gctgatttca cagcctctaa ttcacggtat
     2941 tcctgtttta ataaagttct agatattgtt ctctttccca ctcggaaaca gtttgtctga
     3001 atgccgccca ttcgagacgt tttgcttcaa caaaatttga gaagatatgt tgtcctaatg
     3061 cgtcaatcat tacattatcc ttacgcaatt ctttgatagc attatggata gttgaaggta
     3121 agtcctggat atgtgcagct tctctttctt cctcatccat tacataaatg ttacggtcaa
     3181 cagctgctgg cggtgtgatc tgattacgga taccgtctag tcctgcttgc aacaatactg
     3241 ccattgccaa gtatgggttt gcagatggat caactgaccg caattccaaa cgtgtggata
     3301 aaccgcgaga ttctggtaca cgtactaatg gcgaacgatt acggccactc catgcgacat
     3361 aaacaggcgc ttcataacct ggaacgagac gtttataaga attgactgtt ggattgcaaa
     3421 cggctgtgta ggcacgagca tgtttcagca aaccacctaa gaaatgataa gctgtttcac
     3481 ttagttccaa ttcaccgtct ttatcataga aaacatttcc ttcatcattg aacaaagaca
     3541 tattgcaatg cataccagaa ccattgatcc caaataacgg tttcggcatg aaagtcgcgt
     3601 gaagtccatg cttgcgtgca atcgttttga caacgagctt gaaggtttgg atattatcac
     3661 aagcatctac cacatctgca tatttaaagt caatttcatg ctgtcctggc gctacttcat
     3721 gatgtgaagc ttccacttca aagcctagac tttctaattc caacacgatg tcacggcggc
     3781 aattttcacc taaatctgtt ggtgcaaaat caaaataacc acctttatca ttcaaggtcg
     3841 ttgtgatccc accgtcttcg tctaatttaa ataagaagaa ttctggttca ggtcctaaat
     3901 tgaaagaggt aaaaccaagg tcgcgcatat ctttcaatgc tcgtttcaaa ttcccgcgag
     3961 gatcgccggc aaatggtgtc ccatccggat tatagatatc acaaattaaa cgagcaactt
     4021 ttccatgagc actttcccac gggaagatca tccaagtgga gagatctgga tacaaataca
     4081 tatcgctttc ttcaattcga acaaatcctt caatcgaaga accgtcaaac atcattttat
     4141 tttctaatac tttgtcgatc tggctcactg gaacttctac atttttgatc gtacctaaga
     4201 tgtctgtgaa catcaatcgt aaaaatcgta cgttttcttc ctcaatgatt cgtttgattt
     4261 cttcacctgt tatctgtttc tttaccatgg tatccctctt ccttttttaa tttattcagt
     4321 caaagaattg tctttgcaat ccttgcgact gataatgacc tgttttgctt aacccacctt
     4381 gcgccaaaat ctcatcacga aaaatttgtc gaacgtcatg atctgtcaac ggcttcttgt
     4441 tttttgcttc ttgttttttc gcctgtgcca tttcgtatac acgtttgatc ccagccatat
     4501 tcaatccctc tgaaagatag tctttaatct caagcaacat atcaatatca ttcagcgaat
     4561 acatacgtcg gtttccttcg cttcgttccg gatgaactaa atcttgttct tcataatagc
     4621 gaatttgccg agcagacaaa tcagtcagct tcataactgt accaattgga aatacggaca
     4681 tagatcggcg caattccttt tctcccatca gacgtcccct ttcgttttat aagttggcta
     4741 aagtgtacca attaaaaaaa atattgtcaa ctgcatatgt taggttttct aacatgagat
     4801 ctctattttt tataaaataa caaaaaaata agaacacaaa aaaggtatct agttgatttt
     4861 cgtaccagat accttttatt ttttattata ctgtactatc ctttattagt gtttttat
//
LOCUS       DAPCMT010000167         4836 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPCMT010000167 DAPCMT010000000
VERSION     DAPCMT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.170"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..468
                     /locus_tag="RD743_002573"
     CDS             <1..468
                     /locus_tag="RD743_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDU0564354.1"
                     /translation="SEVLPTIRKTGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEY
                     EPKISYLDTILSSTDTVATSQIAADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMN
                     QGYTKSHTSEIPKADGGTKVVMNTKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            483..686
                     /locus_tag="RD743_002574"
     CDS             483..686
                     /locus_tag="RD743_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564355.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            791..1090
                     /locus_tag="RD743_002575"
     CDS             791..1090
                     /locus_tag="RD743_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564356.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            1090..1272
                     /locus_tag="RD743_002576"
     CDS             1090..1272
                     /locus_tag="RD743_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564357.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(1278..1754)
                     /locus_tag="RD743_002577"
     CDS             complement(1278..1754)
                     /locus_tag="RD743_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564358.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            1807..2532
                     /locus_tag="RD743_002578"
     CDS             1807..2532
                     /locus_tag="RD743_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDU0564359.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            2773..4065
                     /locus_tag="RD743_002579"
     CDS             2773..4065
                     /locus_tag="RD743_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDU0564360.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            4213..4554
                     /locus_tag="RD743_002580"
     CDS             4213..4554
                     /locus_tag="RD743_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564361.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            4547..>4836
                     /locus_tag="RD743_002581"
     CDS             4547..>4836
                     /locus_tag="RD743_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDU0564362.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1736 a          817 c          939 g         1344 t
ORIGIN      
        1 agtgaagtcc ttccaacaat tagaaaaaca ggtagctatt caaacgtacc tcaaagtttt
       61 gcacaagcat tgcgtttagc agcggattta gaagaaaaga accaattact cgaacaacaa
      121 attgccgagt acgaaccaaa gattagctac ttagatacga ttctttcatc gacagatacg
      181 gtagctactt ctcaaattgc agctgattac ggaatgtcgg caattgctct aaacaaattg
      241 ctaaacgagt taggtgttca gcataaagtt agcggacaat ggatacttta ccgaaaacat
      301 atgaaccaag gatacacaaa atcgcacaca agtgagatac cgaaagccga tggcggcact
      361 aaagttgtaa tgaataccaa atggacacag aaagggcgag tgtttattta caacttatta
      421 atcgcagagg gctattaccc tcaaatggat ttagaggaaa ttggttagaa aggagtttta
      481 gtatgactga cattgcagaa atcactcaac gagatagaga aaaaatcaaa gaatatgtcg
      541 aaagttcaaa gttcttaact tacaccatgc ttgctgaaag atttggaatt agcaaaagct
      601 acttatcttt aattttaaac ggtaaaaaga cttctgcaga agcaaacaga attatagatt
      661 cgattatcac tatgtacgaa ttgtagaggg aggtaaagtc gttggaagat aaaatcattc
      721 ttacaaaatc agagcttcaa gaaatgttaa acatcgagta tggaacgata atttttagga
      781 gggataataa gtgaaaaaac caacactttc agagctgata gaagctgctg agaaggcagt
      841 aaaaccagac gactggtacc gacaaagttt aatcttggag aagttccacg gcatgtcaaa
      901 aactacttta gttgaatact gcaaggaaat ggaaacaatt cctgaatttg cagaaggaat
      961 acttcgtcca ggtcactcaa ccacatttat tcattaccat acttttattt ggtttttaaa
     1021 gtggaaagac gcaaacaaat atcgtgtaaa aagtttatcg ccttcagatg ttttgaagga
     1081 ggcaagttaa tgggcaaatt caacagagca ctagtattca gcgcaccgct aatcatctac
     1141 gctttaggcc tctggggaag cagacaagcg ttgataggga caatcgttta catggtttgg
     1201 atttttatgg ggcttgatga agctgagcac agagcgaaaa aagccagtcg ggagggactg
     1261 actaaaatat gattttttta tttttgttcc tttgggctgc tattgcgtac ttcatccagc
     1321 tccttgttgt caaagttgct cttatttctt ttaggaagaa caaaagaaaa tagcgctata
     1381 aatgaaagat aagtagccgc tacattaaac tgttcttttg aaaaagttac agtcatcgga
     1441 atgataattg atattgagga agtaatctta attaaaaata tagcaatatt ttcaacatcg
     1501 cgttggtttt tatttaacat tgtccaacat gttttagcaa caataaaaat aattacacat
     1561 gatattagca cacagactga aaaaagtttt agaaatgcga tgtagtcagt gttattgaac
     1621 tccttcaaaa tactattttt attcaatgta ataaaaggaa tttttttaat tgaagacagg
     1681 aaagataagg taataaacag aattatatat atggttaaag tcgcagtaag aatgtaattg
     1741 agctttttcc tcatagaatc acctcacttt ctaacgaaaa ttataccaaa aaggagagaa
     1801 gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtaactac gagcttacaa
     1861 gtggcagaaa gtttcaaaaa gaaacataag catgtactag aagcaattga atcaataaaa
     1921 agatcggtcg aaaattcggc caatgttgaa gatgggtcca attttggaca gatgtttgtg
     1981 gaagggaacg agccagactc atacggaaga agtcggagag tttatttcat gaatagagac
     2041 ggattttcct tgctagctat gggattcact ggaagtaaag caataaattt caaactaaaa
     2101 tttattgaag ctttcaacga aatggaagat gttattcgga agaatactgt tcctcaaaca
     2161 attgaagaca tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc
     2221 atgcttaaag atactatgcg aattagcgga caacaagagt ttgaaattaa gcaaaaagga
     2281 aatatgaaag ttatggaagt tctaggggga aaagaaagcc gagcttatga agaaatcagc
     2341 aaaaaagtat tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat cccaagatat
     2401 ggcgagttac ctcgtaagag attcgatgat gctgtttcat ttattgaaat gtggttacca
     2461 gaaactgcga tccgcatgga aatcgatcaa ctgaacagac aacaaagact tttcggtgat
     2521 gaaaatgaat agagctgaag cgctaagaat agggacggta attgctaatc gctggtggag
     2581 acacaataaa ccaaacatcc taagccaaca acatatcgaa aagcaaaaag catggcaaca
     2641 aataaaagct ctatactttt tgatgttggt ggatgacacc agcatctttt ttattttcca
     2701 aaacatatag aaaagacacc ttgaaatcct ttagaattaa tttggcgaac caacccaaaa
     2761 ggaggaatca agatgtctta cactcacctt atcaaagaaa cattagatat tctagactta
     2821 tcagttactt ttaacgaaaa ttgtctcaca aaagaaaaat acaaagggca aatctgtcat
     2881 atttatcgtg gaaacttgat ttatacagcc caagaatgca tccactgtaa acaccagatt
     2941 gcttcagata ttgtgcgttg gggtacaaca accgtccggt tgttaatgaa cgatgtttct
     3001 gaatgccgta cctacctcga attgaaaaag caacgcttca aatgcaaagc ttgtcagcgg
     3061 acctttgtcg cagatacttc tgttgcgaaa aaacactgtt tcatcagtga gaaagtccgc
     3121 tggtctgtcg tgactcgtct gaaaaaaaat acctcaatga cagagatcgc cgctcagaaa
     3181 aatctatcgg tctcttctgt ttaccgtatt atgaagcgat tttatcgtcc gttgaatcct
     3241 tttcggacac cgttacctaa ggtactttgt tttgatgaat tcaaatcagt tcggggtgtt
     3301 tcaggtgcta tgagtttcat catgatggat ggacaaacac agcgcttgtt ggacattgtc
     3361 gagaaccgac aactgccttt tttaaaacgt tattttagtc atttttcacg agaaattcgt
     3421 gaagcggtcg aatggatcgt gattgatatg tacgctcctt atgtatcgct cgttaaaaag
     3481 ctgtttccaa aagcacagct gatcattgac cggtttcaca tcgtccaaca tattggtcgt
     3541 acctttcgca atcatcgaat caaagaaacg aaccaactat taaaaagcaa agaacaaaaa
     3601 cactatcaat taggtaagca attaaaaaga tactggaaac ttcttcaaaa agatgaacgc
     3661 aagcttgatt atactcgacg cctttggcga cctggcttca aagctcattt aacagagacc
     3721 gatattgtgg atcgcttact gaaagggagc cctgctttgc gtgtaggcta tcagttatat
     3781 caagactttc tttacgctgt aaaggaacga gactacgtat cgtttgaaga actacttacc
     3841 aataatatca tgcttcccga aggctatcag acgaccttga gaacgttcca gaaatttctg
     3901 ccacaaatca aaaatgcttt acaacaaagt tattcaaatg gcccacttga atgtctgaac
     3961 aaccatatca aagtgttaaa acgaaatgca tatggctttc gtagctttta taatttcaaa
     4021 ttgcgcatca tgattcgtca tggcaacgca ttgattttca actaaaaaag atcaagagga
     4081 actgatccac ttgatctcca attattctaa gatttctctt taccaacatc atttgacaaa
     4141 gagcccaaat aaaaaagtga ctcagccggc aagcatagag tcacaaaaca tatctaagga
     4201 gaattttagc atatgaataa agaactttcc actttagatc aatatttgac tgattctgaa
     4261 tggggcaagt cgaatatcaa ggaaacaaat aatcgaaaaa tcagacgaaa tcttttgacg
     4321 aacgaagaac tatcatgtga tcaagatgac ttgggaaatt ttgtgagtat ttgggatcat
     4381 gtttacctta tccatctatc aaaacattca aataaacctg aatacatcta tgtcatcgaa
     4441 gatggcttga ttgatgcgct agaagagtac aacagagata acttgattga tatctcttat
     4501 tacggatcag gtaagaaata cattgctgaa atggaggcag aatttgatga gtgaaatcaa
     4561 agggacaacg aactttgaaa aactttttag tcgtaagtta aataaaattc tcaagaaaaa
     4621 aggaaatttt gattatttat cttgggctca cgcgtgggag attatgaaaa agaatgatcc
     4681 acaggcaacg gtaactatta atgagtataa acactacagg gttgtttctg gaactcatca
     4741 agactttctt gttgaggaat ataaaccttt tcttatggat gaaacaggga cttatgtatc
     4801 tgtctcagta acggttaaag gacatacgga aacaga
//
LOCUS       DAPCMT010000168         4754 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPCMT010000168 DAPCMT010000000
VERSION     DAPCMT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.171"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            17..1207
                     /locus_tag="RD743_002582"
     CDS             17..1207
                     /locus_tag="RD743_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDU0564363.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     PMEN"
     gene            1341..3530
                     /locus_tag="RD743_002583"
     CDS             1341..3530
                     /locus_tag="RD743_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0564364.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3527..>4754
                     /locus_tag="RD743_002584"
     CDS             3527..>4754
                     /locus_tag="RD743_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDU0564365.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         1707 a          728 c          862 g         1457 t
ORIGIN      
        1 aagagaggac ttccctatga acgattttac tacagaaatt ctaaagactc tagcgaacaa
       61 aggcgatttg aatgaattat tccgtgtcca tttggagaaa gcagtcaata cgcttctcaa
      121 aacggagtta acggctttcc tagattacga aaagtatgat cgcattggtt ttaacacggg
      181 taattctcgt aacggctcct atgaccgtac ggtcaagacc gagtatgggg aacttcatct
      241 ccagattccg cgcgaccgca atggtgagtt caagcaacag actgttcctg cttatagacg
      301 gacgaatgac acgttagagg agaccgtcat tcacctcttc cgaaaaggta ttaccatgtc
      361 ggaaatcgca gacttgattg agaaaatgta tgggcattac tacacgcccc aaaccatgtc
      421 caatataaca aaatcattta cagaagaggt aacggcgttt aatgggcggg agcttcatga
      481 ccgttatgct gctatttata tggacgcaac gtatattccg ttaaagcgga aaaccgtcgc
      541 caaggaagct attcatatcg cagttggcat tcgcccggac ggatcaaagg aagtattgag
      601 ctatgcgatt gcaccgactg aatccatcac gatttgggag gaaattttat tggaccttca
      661 agagcgcggt ttgaaaaatg tcctcctgtt catcacggat ggcttaaagg ggatggtagg
      721 agcgattagt cggttctatc ccaaagctcg ttttcaacat tgttgtgtac acgtttcccg
      781 taatatcagt cataaagtgc gtgtcgatga tcgtaaggaa gtctgtgatg attttaaaat
      841 ggtgtatcaa gcctcatcta aagaggtggc gttggaagca cgtggtactt ttgcggaaaa
      901 atggaaaacc agctatccaa aagtggttga atcgattctt tcgaacgatc acttgctcac
      961 tttctatgat ttccccttgg ccatacgcaa gagtatttat tctacgaact tgattgaatc
     1021 ctttaataaa caaatcaaga aatacagcca ccgcaaggaa cagttccaaa atgaagagtc
     1081 gatggaacgt ttcttagtct cgtcttttga tacttacaac caaaaattcc taggtcgcag
     1141 tcataaaggt ttccaacagg ccgaaggcga gctcgaacaa atgctgagtc aaccgatgga
     1201 gaattagaaa caatctgcag tagggaagaa tcatttacac aaaattattg acgctccctt
     1261 ataatgagaa cttttactaa cataatttat gataaactta ctatataatc atattaatta
     1321 aatagttagg agatctgctt atgtttatat ctactcttca aattcgaaat tttagaaatt
     1381 atgaatccga aatctttcga ttcgataatg agacaaatac tattataggt gagaatgatt
     1441 cagggaaaac caatgctctt actgcattga gaatcttatt agatgatagt tattactata
     1501 gtagtaaaac actaaaagaa tcagattttt ttcatggaat tcaaaatggg tggcaagggc
     1561 attggattat tatctctgcg acatttgaag gaatcagcga agaagaattt gacaatgaga
     1621 tttgtgcttc catttcttta aattctgaaa gtcaaacaat cttagaagaa ttgatttcaa
     1681 atgcagataa aggtgtaggt agtatttctc tatttattag acctaacaaa gctattagaa
     1741 agcaattatt tgatatttcc actgaattag actctcatca atttaacgaa tttagaaatt
     1801 ctattcgtct atccgactat gaattttact acacatctaa atcaaatcta aatttctgta
     1861 tcaatgaaaa ttatgataaa ttggtaggaa gactcaatga caatatagct agtaatcctg
     1921 aagaagacga tgaagctctg ttgggctcaa gaattgacat gtcagatatt ttcaagcata
     1981 tttcggttgt ttatgttgac gctcttcgag atgttcttag agaaatgaaa aacaatcgaa
     2041 acccagtcaa aagaatcatg gaaacaattg aatcaaaaat atcatcagat aatgttgagt
     2101 cattaaaaac aattatacag caattaaatg agactattac cagtgttcct gaaattagga
     2161 aaattggcga aaatattaat cggcaactaa atagcataat tggttcagtg tattctccta
     2221 atcttcttct ttcctcaact atgtcagatg atatgggttc tttagcaaaa tttctttcaa
     2281 tgaagccaga acaaaatatc gacttagact tactagggtt aggacatcta aatatgattt
     2341 atttagcttt gaaaatagtc gaatttgaag cgtgtcgttc aagagaacta cttaatatta
     2401 tgttgattga agaaccagaa gctcatattc atcatcatat tcaaaaaact ctgttcgaag
     2461 gattaaatct tcaaaaaaac tatactcaga ttctcatgac cacacattct gtacatttag
     2521 ctgaagtgtc tgaaatatct cggatgaatg tattaaaatc gtttaatggc aaatctatcg
     2581 ttatgaatcc agcaaataat ctgaactcat ttgctaaaga aaagttaaaa aagaacaatc
     2641 taaatctaat aaacgcagta gagagatatt tagatgttaa aagaaatgga ttgctttttt
     2701 ctaaaggtgt tatcttagtt gaaggagatg ctgaagaaat acttattcct caaatagtaa
     2761 aaaaattatt tggtataaac ttagacgaac ttggaatagg tttgattaat gttggtagta
     2821 cttcgtttga atatatcgct agcttgtttg acgatgatag aatacagaga aagtgtgcta
     2881 ttgtaactga tttagatgaa caggcaatcc catcagaaca tcgattgtat aaaagcactg
     2941 cacaagagaa aggtcaagca agaaaagaaa aattatcctc cctttttaat gaaaacaatt
     3001 gggttaattg tttttatgct aatacaacat ttgaaattga gtttttagat gtgaataaag
     3061 aaaatattct aacttatgtc agtccaatta tagatgacgc attcaaagat aaaaaaacaa
     3121 taactgctta taaagaagaa atacaaagtg aacaatattt agaaagaaat ttagcgatgt
     3181 taaaactagc caatcatgtt ggtaaggggt ggtttgcaat tgagttaggt gaaaaaattg
     3241 actttttaat caaaattcct aagtatattc ttaatgctat tgcttttgca tgtcaggaaa
     3301 tagtagatct ctcaatttat aaaaaaattc tactccatac aatcgacttg tacgaggaaa
     3361 ctacagaatt gtcagcttta tcagctagtt taaaaacaag tgatacagct gatttgaata
     3421 tagcaataat ttctttatta gagtactttg gtaaagatga ggatgttgtg tatctaatca
     3481 atcagataga aaattacaca aaaatacttt atgctgaggt ttcaaaatga caaatatctt
     3541 cgaaaaaatc gtaaatgatt taaatttaga acaaaaagat gttgtggaaa gtgaagataa
     3601 cctttatgta aatgcatgtc ctgggagtgg gaaaacaagg gtacttacaa ggaaaattgc
     3661 ttaccaagca attaaacacg atggatcact aaaaagaata attgctatca catacactaa
     3721 tagagctgct gaagaaatta aggaacgcct aagtttactt agtatagatg atgatattaa
     3781 tatttgggtg gggacaatac atcagttttg tcttgaattt attatttatc cttttaaaat
     3841 gaatctctca agagtatcaa aaggatttac aattattgat gattatactc aaagagctta
     3901 cgtcagccaa attaataata tgctgaattt aaatctcaaa acgtatgaac tgaacaaaat
     3961 tgatttgtca ctttctcatg attttaaaat taatgaagtt cagtatactc aagtttctga
     4021 aatgtatcac actatgttgg aaaaaaataa agaaattgat tttgatatga ttttactagt
     4081 agcgtataaa attttaactg taaacagtaa cgttgcacgt aatattgcta gtaatataag
     4141 ggcgatttat gtggatgaat ttcaagatac tagagaatta caatacaatg taatagctaa
     4201 attacttcaa aacaaccctc aaattcagtc aatgtttgtt ggagatattg accaagcaat
     4261 ttatggatca ttagatggta tagctaaatc agttgaagaa ctagaaatgc taactagtca
     4321 cacttttcaa tcaaagacgc tacatggctg ttacaggtct aatcagagat tagtagactt
     4381 ctactctaat tttcagagtt gtccttacga gattgaaagc agagggaata ataaaaacga
     4441 ccgtggtttt ataagttatg attgcaaagt tactaaggag gatttaccca acataataaa
     4501 aaatataata aaggaaaaga ttgaaagcgg cattcatgaa aaagatattt gtgtacttgc
     4561 tcctcaacac tatcctctgt tttcactggg tgagtattta aagaaagaat taccgtattg
     4621 caattttgat gctataaata tatcaccaat taaggtaaat aatcatagtt tatttttcaa
     4681 gttagcaatt ttgtacttta ctgaatcagg tgaaaaggtt cgaagaagaa agctaattgc
     4741 aaacgatatt ttaa
//
LOCUS       DAPCMT010000169         4711 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPCMT010000169 DAPCMT010000000
VERSION     DAPCMT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.172"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            106..357
                     /locus_tag="RD743_002585"
                     /pseudo
     CDS             106..357
                     /locus_tag="RD743_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="RD743_002586"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="RD743_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDU0564366.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="RD743_002587"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="RD743_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="RD743_002588"
     CDS             1786..1869
                     /locus_tag="RD743_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDU0564367.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="RD743_002589"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="RD743_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDU0564368.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="RD743_002590"
     CDS             complement(2994..3857)
                     /locus_tag="RD743_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HDU0564369.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="RD743_002591"
     CDS             complement(3859..4131)
                     /locus_tag="RD743_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDU0564370.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="RD743_002592"
     CDS             complement(4148..4357)
                     /locus_tag="RD743_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDU0564371.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4711)
                     /locus_tag="RD743_002593"
     CDS             complement(4455..>4711)
                     /locus_tag="RD743_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HDU0564372.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc a
//
LOCUS       DAPCMT010000170         4710 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPCMT010000170 DAPCMT010000000
VERSION     DAPCMT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.173"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            161..391
                     /locus_tag="RD743_002594"
     CDS             161..391
                     /locus_tag="RD743_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDU0564373.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="RD743_002595"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="RD743_002595"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDU0564374.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="RD743_002596"
     CDS             1802..1978
                     /locus_tag="RD743_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDU0564375.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="RD743_002597"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="RD743_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDU0564376.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="RD743_002598"
     CDS             2593..3060
                     /locus_tag="RD743_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0564377.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="RD743_002599"
     CDS             3145..4029
                     /locus_tag="RD743_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0564378.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..4379
                     /locus_tag="RD743_002600"
     CDS             4143..4379
                     /locus_tag="RD743_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDU0564379.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="RD743_002601"
     tRNA            4467..4547
                     /locus_tag="RD743_002601"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="RD743_002602"
     tRNA            4554..4625
                     /locus_tag="RD743_002602"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
BASE COUNT         1577 a          737 c         1028 g         1368 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa
//
LOCUS       DAPCMT010000171         4614 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPCMT010000171 DAPCMT010000000
VERSION     DAPCMT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.175"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(86..340)
                     /locus_tag="RD743_002603"
     CDS             complement(86..340)
                     /locus_tag="RD743_002603"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDU0564380.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="RD743_002604"
     CDS             complement(451..705)
                     /locus_tag="RD743_002604"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564381.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="RD743_002605"
     CDS             complement(898..1173)
                     /locus_tag="RD743_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDU0564382.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="RD743_002606"
     CDS             complement(1145..2098)
                     /locus_tag="RD743_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDU0564383.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="RD743_002607"
     CDS             2710..4203
                     /locus_tag="RD743_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDU0564384.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4614
                     /locus_tag="RD743_002608"
     CDS             4338..>4614
                     /locus_tag="RD743_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDU0564385.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRK"
BASE COUNT         1495 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga aaaa
//
LOCUS       DAPCMT010000172         4522 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPCMT010000172 DAPCMT010000000
VERSION     DAPCMT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.176"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..943)
                     /locus_tag="RD743_002609"
     CDS             complement(<1..943)
                     /locus_tag="RD743_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0564386.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISI"
     gene            complement(945..2384)
                     /locus_tag="RD743_002610"
     CDS             complement(945..2384)
                     /locus_tag="RD743_002610"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDU0564387.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2569..4515
                     /locus_tag="RD743_002611"
     CDS             2569..4515
                     /locus_tag="RD743_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDU0564388.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1427 a          776 c          753 g         1566 t
ORIGIN      
        1 gtattgagat tggtaaaata taatccttat ttgtcttttc gcctttaatt tttttcagaa
       61 gaatatcaac aataagactt gcaatttcat caattggttg tcttattgtt gtcaattgtg
      121 ggaaaaattt ctcaataaaa gaggtaccat cgtatccaat aatttttaaa tcttctggga
      181 tattaagttt aagttgatga gcaattttca tagtgagaat ggctgttaaa tcatcagaaa
      241 caaaaatacc atctggtttg tgatttataa tagttgactt tatttccatt tcacgacgga
      301 tagtagacag attattagga attttgaaaa tttttccatt tggattttga aaagaaaaac
      361 ctagtgcacg taattctgtg ggagaatccg tgttatcatt tcctgtaatc ataatggtat
      421 tttgacaacc atttttttgt aaagtcttag ctgcaagttt tcccccttca aagttatctg
      481 aagaaacaat ggaaacatta ggagctaaat ttctatcaaa agcaacaata ggtgctctca
      541 cacgctcata atcagcaatt ccaagattat ggcttgatga gattatgcca tcaacttggt
      601 tggcttcaag catttcaatg tattctctct ctttagcagg atcattttga ctattgcaaa
      661 taatcgtttt atatccatgt ttaaataact ctatttcgag atactcaatg agttcagagt
      721 aaaagatatt actaatatta ggaaaaataa gaccaataag ctgaggtgac tttccttgaa
      781 gtccacgtgc taaattattt ggtttataac caagagtttt catagcttca tgaacttttt
      841 gttttgtttt ttctgataaa tatcccttat tattaatgac gcgtgaaacg gttgttgggc
      901 taactccagc taattcagcg acatcagtta attttgctac cattttaata ttttaattca
      961 aagtattttc cagtaggttc gcctgatttg atttgaatac cattttggtg attttcaggg
     1021 aatacacgac tagtaaacac tttttctcct ttattaataa aaatctcaat gattgagtta
     1081 tctacaaaaa tgttagcagt tgtttcttta gtatcaattg agcattcacg aatcgtacca
     1141 aagtcagtgg catattgaat gcctgctttg ctgcgatcta aaataatttt tccttctttg
     1201 gtatctacaa tcaagctaag tccatttcct ttactatcag agaaaagaag taactccgat
     1261 ttctgatttg gaggaaaagt tagttcaagc tcataagtat tatttgtttc tgcttttgct
     1321 gtaaattgtt cagaactagc acgtaatgat gtaatagctt ctactggata ttggtaaagt
     1381 ttaccatctt ttattgaaag ttcttttaca agactcattg ctccctgata atcatactta
     1441 tcagttggat aatcgatatc aggtagtcca atccaactga caatcagagc acgaccatca
     1501 ggagtattga atccttgagt cgcatacgct tcaaatccat aatcaagatt ttgaatttct
     1561 gaagcgccca caagttttac tttttctatg tcaagcgatt gacagatctt aaaagtattc
     1621 ggatagatat tcccataatt tagctcagat ttatccaacc cttgaggaca ataaatgaga
     1681 acaggttttt tgtctacaaa tactagattt ggacactcaa tcatgtattc tgaaccagtg
     1741 ccgccaaaat caaggttgcc aacttcctcc catgtttcca cgttattgtc atccgctttg
     1801 taaagtttga taaagcctga tttttctaaa ttttgtgctc caacaatcgc atacaattgt
     1861 ccttcataat taaacatttg tggatcacga aaatgatcag ttacatcagc aggtttatga
     1921 ataagaacat tctcaccttt cgtaatgttg tagtctttat ccatccatgc tccgatttga
     1981 agtgtatcac gttcccaatt ttcatttcga acgtttcctg tatagagtaa gaatagattc
     2041 ccctctattt catatgcaga accggaataa gcgccgtggc tatctcgttt tgtgtctgga
     2101 agaagttctg ctcctgttac atgaaaatga actaaatcat ctgattcatt gtgtacccat
     2161 tctttcaacc catgagcagc tccaaatggc caattttgat aaaaaagcgt atattttccg
     2221 ttaaaaaaag aaaaaccatt aggatcgttt aacaatcctg ttttaggttc gatatgatag
     2281 ctagcacgcc aaggtgatcg ttttacatta tccttaattt tatccatctc atctttagtc
     2341 caatcagaat aagcacgata tcgtatttct cgaggtaagt tcatatttta ctccttcaat
     2401 ttatcttttt acaactagta tggtaaacgt tttccgttga aaaatcaatc atttcattta
     2461 aaaaaataaa ctatatgaca aacgtttgac atatttttaa gatatgctaa aataaatttg
     2521 catttcagtg aaccgttttc acgtaaaaat aaaaaaggag tgattcgaat gaattacagt
     2581 aaagtagctt ccgacgtaat acaagcggtc ggaaaagata accttattgc agctgcacac
     2641 tgcgctacac gtcttcgtct cgtactaaaa gatgacacga aagtgaatca aaaagcttta
     2701 gacgaaaatc cagatgtgaa aagcacattt aaaattaatg gacaatatca agtgattatc
     2761 ggtgcaggtg atgttaattt cgtttatgac gaacttatca aaaaaactgg actttcggaa
     2821 atttcaacag atgatttaaa acaaattgct agtaaagata aaaaattcaa ccccattatg
     2881 gcattaatta aattattatc agatattttt gttccaatta tacctgccct tgttgcaggt
     2941 ggtttattaa tggcattaca aaacttttta acagctgctg gactttttgg accaaaatca
     3001 attgaagaaa tgtatcctgc gattgaaggt atttcctcaa tgatccaact gatgtccgct
     3061 gcaccattca tgttcttacc aattttagta ggtatttcag ctgctaaacg ttttggagca
     3121 aaccaatttc ttggtgctgc aattggtatg atcatgacaa caccagatct tggtggctca
     3181 actgaatatt ggaatatttt tggttatcat gtttcacaaa caaactatgc ttatcaagtt
     3241 attccagttt tagcagctgt ttggatatta tctatattag aaaaattctt tcataaaaaa
     3301 ttgccatcat ctattgactt tacattcacg ccattattat cagtaatgat tactggattt
     3361 ttgactttca cagttattgg ccctgtaatg ttattgttgt ctaatggaat tacagacatt
     3421 attgtttggc tatataacac tactggattt attggaatgg gtatttttgg aggaacatat
     3481 tcacttatag tcatgactgg tttacatcaa tcatttccag ctattgaaac tcaattactt
     3541 tcagcgtgga caaatggtat cggacatgga gactttatct ttgtagttgc atctatggca
     3601 aacgttgctc aaggtgctgc aacatttgcc atttggttct taactaaaaa ttctaagaca
     3661 aaaagtcttg cttcctctgc gggtgtctca gcacttcttg gaattacaga gcctgccttg
     3721 ttcggagtta acttaaaata ccgctttcca ttcttttgtg ctttaattgg ttcaggaatt
     3781 gctacagcag tagcaggatt acttaaagta gttgctgttt cacttggttc agcaggtttc
     3841 ttgggatttc tttcaattaa tgcaacatct attccatttt acgtattgtg tgaattgatt
     3901 agctttgtag ttgcttttgc aattacttac ttctacggta aaacaaaagc tgctggtatc
     3961 tttaaagcag aagaaattgc tgaacaaaca ccagttaatt cttcaaaagt agcaacaaca
     4021 gatccaatat ctactgatgt tgaatcaaca caaacaaaag tttcatcaga aacaattgtt
     4081 agtcccattt ctggtaaagt gattagtctt tcttcagtaa atgatccagt cttttcatca
     4141 gaagctatgg gaaaagggat tgccattaaa ccaaatggaa acacggtcca ttctccagta
     4201 gatggtacta ttcaactcac gtttgaaact ggacatgctt atggtattaa atcaaataat
     4261 ggtgctgaag ttctaattca tattggtatt gatactgtat caatgggagg aaaaggattt
     4321 acacaaaaag ttactactaa tcaaaaagtt agaaaaggcg atattcttgg agcatttgac
     4381 cgttcaatga ttgagaaaga aggtcttgat gatacaacaa tgattattgt gaccaatact
     4441 tcggattatt cagaagtaaa tatagtagct gaaaatacag tcactgaggg tactgattta
     4501 cttgaaatca aataaatata tt
//
LOCUS       DAPCMT010000173         4439 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPCMT010000173 DAPCMT010000000
VERSION     DAPCMT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.177"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..606)
                     /locus_tag="RD743_002612"
     CDS             complement(<1..606)
                     /locus_tag="RD743_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDU0564389.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNM"
     gene            complement(736..1320)
                     /locus_tag="RD743_002613"
     CDS             complement(736..1320)
                     /locus_tag="RD743_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0564390.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1651..1884)
                     /locus_tag="RD743_002614"
     CDS             complement(1651..1884)
                     /locus_tag="RD743_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDU0564391.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1899..2849)
                     /locus_tag="RD743_002615"
     CDS             complement(1899..2849)
                     /locus_tag="RD743_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDU0564392.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2846..3688)
                     /locus_tag="RD743_002616"
     CDS             complement(2846..3688)
                     /locus_tag="RD743_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDU0564393.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(3690..4409)
                     /locus_tag="RD743_002617"
     CDS             complement(3690..4409)
                     /locus_tag="RD743_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDU0564394.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1505 a          811 c          545 g         1578 t
ORIGIN      
        1 catgtttgtt ttatagtaag aacttctttt cacaggagat tctatcccct ttgttattgc
       61 attagcggtc atttcttcag caatcatagt tgcacgtata agaacgggaa ctaacagaaa
      121 ttccaaatat tgcagaggct gaaaaaaaga caagcgaatg tttctcattt ttaaagattc
      181 tttaatcatt cttatttcag aacgataagt cggaaagaat ctgatcacta ctgtaatact
      241 taacattatt tttttcggta aatgtatttt ttctaaagag gataataatt cattgctagt
      301 atagacacta accaacaacc atgagatcat tacgaccggc aacattcgaa atacaatata
      361 taaggatgtc gcaaaaaata tagtaaaaaa ctctatcttg atagcatcaa taattttcat
      421 tactaaaaac agaactgaat aaatcaagat gaaataaata catgtcttta ttttacgaaa
      481 catcaatact aaaactgtag caataatgaa acaccccact tgaaatatat ttccctttcc
      541 taaaaaaata atgaccggaa aaaaaatatt taaagtaatc aaggttaacg gattgatttt
      601 tgacactctt tcacctccaa aaaataagat cgcactgtta tcttaagctg ataaaaatgc
      661 accgttattc agataacagt gcgatcttat caaaaagcat aactgagaac aattctcaat
      721 gcaagctttt tttaattaac tcagtaattc aatccaccca ggaacagaaa attgatacaa
      781 tcttctggtt aatagcatag tcttttcttt tgaatagcta tgcttcaata tatcatagaa
      841 tgtagcgtag aatgagtaaa tgtagaagtg gatttcttct tcattaaaga ctaaattttg
      901 attgatcatt ccgatagtct ttaacttatc tagacaacag actgtctttt tcgtcacaaa
      961 tgatgtaatt ttttcaacaa tatcttgata tttggttcca gctgattgga ataaaatcaa
     1021 atcgaatata cgtttattct caaaaatata attaataaat ggatctaatt cattgaagtt
     1081 ctctctccaa cttttctcta tatctatatt ttctaagttt aagaggtatt tggtaaactc
     1141 ttcattatac atattaataa gcgcattaat atgcggctga ataatttctt caaataacgc
     1201 ttccttagaa gtaaaatgtt tataaaaagc acccgtagtt acgcctgcgg tcaggcacag
     1261 ttgccttatc ttagtttgct gaaatccttc tttttcaaaa tggcttagtc cactttctat
     1321 taacttttgg tgagttataa tataatctct ggtttctatg ggtacttcct cctaataaga
     1381 tgaattaact ttattatctt acacaggact tgaagtttca atcgttaacg tttattactg
     1441 aactcattcc ttagagctaa actaatgaga aagagcagaa taactgatac aaaaagcaat
     1501 cctacaagaa ttaatagact catctaaatc attcaccttt tctaaaataa tccgagaaag
     1561 atttaaaaaa attatatcca gctattagga taagtcctat cataatcact ccaatagcca
     1621 ttttcttatc ctccctaaaa ataaagtctt ttacttaaaa ccttttttgt aatcgtcaat
     1681 aaattttttc cctttatcag ttaattgaaa tcctatatct aaatagtcct catatctaag
     1741 tttagccagc atctcactta cagatgctgg actaacactt agaacttttg ctaaatcatt
     1801 attttttaat ttcttttttc tttttgataa ttcctgaatt gctagtaaat aaattagtat
     1861 agtagtcgtt ttaatttgta ccatacctac cctccaaatt atttcgaaag gccttcagca
     1921 attttatcca ggttccattt catcatcgca tagtaactat caccatcttg tcctttttct
     1981 gcaattgaat cagtaaaaat cgttgaatag ataggaatat tggtatcttt tgatactgtt
     2041 ttcattggtc tatcatccac actgctttct acgaataagg agggaacttt tgttgtgcgt
     2101 aatttttcaa ctaagtgttt tatttgatct ggtgttcctt cttcttcagt gttgatttcc
     2161 caaatgtaag cagaaggcac attatacgct ttcgagaaat atttaaagca tccttcactt
     2221 gtaacaatca tttttttatc attcggaatt gaagcaaatt tagatttagc ttctttgtct
     2281 agagaatcca atttttcaat atacttatct agattttctt tatagaattt tttattatca
     2341 ggatcttttt ccgctaactg tttttcaata tttttagcgt aaataatacc gttttctaaa
     2401 tttaaccaag catgaggatc ttccttccct ttctcactct gaccctctaa gtaaataaca
     2461 tctatgccat cacttgctgc aaaatagtct ttgttttcct ctttgttcgc atttttaact
     2521 aattttgtaa accaagcatt tcctccagtt tccaagttaa caccgttata aaaaatcaaa
     2581 tctgcttttg aagttttttg aacatcttca ggcaaaggtt catattcgtg gggatctttc
     2641 ccaataggta cgatactgtg taaatcaatt ttatcttttg ctatattttc ggtaatatct
     2701 gctaaaatcg aattagtaac tactactttt aatttttcgt tcgaagccac tttatctttg
     2761 tctttactat ttattgaaca tgccccgaat ataaaaagag caatcaaaat ccccagactt
     2821 cccagtactg ctttccattt tctcatcata acgatctctc cttctttctt aaaaattttt
     2881 gttttggtga gattagaaaa ctaattacaa ataagaatgc cgctgttaaa acaatacttg
     2941 atcctgctgc tatgttaaaa ctataaccaa taaacaatcc aagcaatgat gaacttgctc
     3001 cgactgttgc agaaataaat atcatagttt tcaagctatt tgcatataaa tatgctgtag
     3061 ctgctggtgt aattaacatt gcaacaatta atatagttcc cacactttgc atagctgtta
     3121 ctgctactaa agttaataga accatcaata gataatgata aaaactgaca ttcatcccta
     3181 ttgcttttgc cattaatgga tcaaaagaag tgattaataa ttctttaaaa aatagaacta
     3241 ctataaccaa cactaaaacc gaaacgccaa ttgtaatcca cttatcaata tcttgtacag
     3301 ctaaaatatt tccaaataaa atatgaaata aatccgttga gctatttgca actcctataa
     3361 ggataactcc tagtgctaaa aaagaactga aagtaatacc aattgctgta tctcctttta
     3421 ttacactatt gctttttata aaagtaataa tgatagaagc taatagacca aatacaatcg
     3481 caccaataaa aaaattaata cctaaaatat aagaaattgc tactcctggc aaaacagcat
     3541 gagaaatagc atcacccata agagacattc ctcttaaaat aataaaacaa cctactgttc
     3601 cagaaacaat ccctataact acagatgtaa ttagagcatt ctgtaaaaag tgaaagtcat
     3661 acagaccatt aataaaattt tgaagcatat tattccccct ctccaataaa tatagtatct
     3721 ccataggctt tttttaaatt ttcttcatta aatacagatt ctacacttcc gtgagcaatt
     3781 agatttcgat tcaaaagaat aatattatca aaatacttct ttaccttgct taaatcatga
     3841 tgcacaatta gcactgtttt tccctgatgt ttcaattttt ttaacgtatc cataattatt
     3901 cgttcactaa ctaagtctat ccctacaaat ggttcatcta aaaatatgaa atccgcattt
     3961 tgagcaagac atcttgctaa aagtacacgt tgaaattgtc ctccagataa ttctcctatt
     4021 tgatgattag aatattttcc catatcgacc ttttccaaag cctttgaaac tctttgccat
     4081 tctgcatttt taattctttg aaaaactttc attccggcat atgttcccat agatacacat
     4141 tcttttactt ttattggaaa agtaaaatca atatcagctt tttgctctac atatgcaatc
     4201 ttcaccagtt ttttattgat atcttcatta ttaatggaga cctctccctg atgagggata
     4261 atatttaaca ttgctttaag caatgtggat tttccagcac catttggacc aataatacct
     4321 gttaaagcac cttcttgtat ttttaaagaa atctcgttca atgctaaaaa atcgttataa
     4381 caaacattaa gctttttcac ttctaacata actttctcct tctaaatgat aatgattat
//
LOCUS       DAPCMT010000174         4422 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPCMT010000174 DAPCMT010000000
VERSION     DAPCMT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.178"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            270..1001
                     /locus_tag="RD743_002618"
     CDS             270..1001
                     /locus_tag="RD743_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDU0564395.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1146..3173
                     /locus_tag="RD743_002619"
     CDS             1146..3173
                     /locus_tag="RD743_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDU0564396.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3281..4378
                     /locus_tag="RD743_002620"
     CDS             3281..4378
                     /locus_tag="RD743_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDU0564397.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1550 a          715 c          920 g         1237 t
ORIGIN      
        1 taggcagtca agtcacattt tgatagatag gagggggact tacgaattat tttaaaggct
       61 atgacagctg tatttctaga ttgtgtttgc atcaagttgc aataaaaact agaaatcaaa
      121 tcgggctgat aatcatttat aactaatcga tacataggac caaatatagg tctatgttct
      181 tttatttcat aagtatcgta aaacagaatt ttgctatgga ttaatccaat cgactgatat
      241 gtttagtaaa taaagaaaga ggaatgtcaa tgatgaaaaa agaaatcgta ttaacagcag
      301 caatgatgtt attcagcatg gtagcaagta cgacttttgt ttctgcaact gaagtatacc
      361 caaaggaata taacacagaa ggaacgatta catttgaagc aggagatgag ggtgtaactc
      421 ctcctgttga tccagagaat cctgatccca ataaacctgt cgatccatca gatcctccaa
      481 gccctggaac aggaggggca ttatcgattg attatggatc aaaattcaaa tttgggactc
      541 aaaaaatttc tactgcggat aaaacgtatt atgcagctgc tgatgtgatg aacgatgggt
      601 ctagaaaacc gacatatgtc caagtgacag ataggagaag taccttatct gggtggaaat
      661 taagtgtctc tcaaccagaa cagtttaaaa ctgccagtgg ggatgaacta gttggagcac
      721 agctgaaatt tacgaaaggg caggctgttt ctttggttga tccaacatat acgccgcaaa
      781 cggtgaattc tgaattaacg ttgacaccgg gaggaaacaa tacgttagca attaatgcta
      841 aatcaggaac aggtgtgggg acttgggtct atcgatttgg tgcaaatgaa aatgaaaatc
      901 aagatgcggt acagctttcg gtacccggta aatctgtcaa gttagcgcaa caatattcca
      961 ctaaattagt ttggacatta gaagatacac ctaacaacta gtttactgaa aagaactgtt
     1021 aaaaataaat acgtagtcat attgtcttgc gaggtaatcg agcaaaaatt gtcaaaggct
     1081 aggctaaatt tgtttggata cgaatagttc atctaggaat ttaacgatca ggaggaaaag
     1141 atatattgaa aaagaggagg atcgtgctca ttttaagcag tctcttagtg actaatatct
     1201 ttccgatcat agggtactct ttggaattga atcagttgga gcatggaaat agaagagacg
     1261 aagaaagcaa actcacttcg acattcgaca gtttaataga atccaaccag acacaagaaa
     1321 gcagtgagga tttaagtatt tcgagtagtg gagagaccac tgacaacgta gaagctgagg
     1381 gttcggacaa acaaggacta gaagatgcac caatttctgc ggttgagctg cctgacgatc
     1441 ccaatattat ttctatcgat aaaatttttc aaacacctat cggtactagc acgtccatat
     1501 tggaaaatgg gaagttactg cagttgaacc cagcagaaag gtctcaaaga ggagctattt
     1561 ggtcaaataa gaagattagt ttactctcgg attttacatt taaatcttat ttatacttag
     1621 gaaatcaacg agatagagcg ggagatggca tgacctttac attgactggc gatcctagaa
     1681 tggctagtaa tcccagtcaa gttattggat cgccaggtat gggaattggc tcatattcta
     1741 ctcgttctgg ccaaccttac attgcgaatg cgctctcgat tgagtttgat acgtataaaa
     1801 atagtggttc ttctgatcga atggatcgag agataactgc tgataataga gggcatggac
     1861 atgtcgcttt tgtcacgcct aaaccaaata ataataatta ttctggagag catactggcg
     1921 tgacggtagc tcctacattt ctttctaatg gcacatggcg cttgttaata attaaatggg
     1981 acgcggagca aagaagtctt acttatgatt tggaaggtgt cggtcactct tctcggatta
     2041 tacctgatct cattaggcag tttggttcta cagaagtaaa ttgggggttt acttcttcaa
     2101 caggtacttt ttttcaagaa aatgctgtcg ctgtcacgcg acttccatcg aatgtcacat
     2161 caacagctga tgtaaaagtc aacgatagtg attatggggc catcgctgaa gctggaggaa
     2221 acgataagat aactatcaga aatacattga aggtcgaggg ggattttctt gaatataaga
     2281 aaatgcagac tagtatcgac ctcccacaag aattaactat tcccgagcat atcttgatca
     2341 atggaaatca aattgatgcc aaagatatct tgataaccgg aaatcgagtc acgctaaatc
     2401 tgacagatta ttttattgct ggagatacgt taataatcga attggagaca actttgcagg
     2461 aagtaattcc tgaaaaagta ctcactatgc attttgaata ccttgaaaac gggaatttga
     2521 ttgagcaatc gaatgaaata aacctaaaga tcgcaaagct gaaggagata gatatccatg
     2581 tttattataa agataaagac agtcagattg atattgctga aatgaaaaca atcactggga
     2641 aaataggaga gaagtataaa gaaactccta ttgatattga agggtatgtt tttgactcgg
     2701 attcagcgaa tacagaaggc atcttctccg aaaatacaga ggatatctat ttttattaca
     2761 gaaaaggcaa actttatctc tcaactgctc ctcaatatgt caattttgga aaacataaaa
     2821 tatccagtag accattaagt gaattcggac agttttcagg tgggttgaaa attatagatg
     2881 aacgggcttt tggaagttgg aaattacaat tgaaacaaga gaaagttctt acaaatggag
     2941 attttgagct tccagaagct ctttcctttg ttacctctga aaatacaacg gttataggga
     3001 catccgctgt tacgattttc gaaagtgatc aaaagggtga aagcgatttg tcctctcttt
     3061 tagattctac agctcatcgt ggaatcagaa tagatattcc tgtagaaaac caaagaatcg
     3121 gaacctttga ggggaaacta tcttggatac ttgcagatgt cccaggaaat taacaggaag
     3181 agaagaatat aacaaaatta ttgagtttca agaagggcac aggtaccata ggctcttgtg
     3241 tcccacagag acaagatttt gttatagtct tctcttacat atgattaaag gagcgatttt
     3301 cttgagacca aaacaacaat tatttataac actcctagtt attttagggg cactttttaa
     3361 caatatatca gtgtcagcta gtgcatttaa tttttcagtg acgccgataa cttcagagaa
     3421 tcaaatcgac aagagaaaaa cttacttcga tcttcaactt gatccggatc aggaagtaga
     3481 agtaaaagcg gaattgagaa atgatacaga aaaagaagtg aaaatcgata tttctgtgaa
     3541 tagcgcaacc accaattcaa atgtgatggt tgaatacgga aaaaacgaga ttgaaaaaga
     3601 catgagcctg atctttgatc tggtagatta tgtgagctat ccacagatag tgacgctaaa
     3661 acctaaaagt gtccaaaccg ttgcttttca tgtcaggatg ccgaatgagc gatttgatgg
     3721 tgtattggct ggtgggatca cattccaaga aatccagacg gaaaaggacc aaacagaaac
     3781 taaggatcaa agcttatcga ttgagaatga atacacctat attattgctt tgttgatgca
     3841 acagacactg aacgaagttg ctccgaacct tactcttcat gaagttaaac cagatcaaat
     3901 caatgcaaga aatgtcatct tagcaaacgt tcaaaatgat caaaaaacct atatcaatca
     3961 agtagttatt gaaacaaaaa ttacaaaaaa aggacattct gaagttttat atcaagagga
     4021 aaaagaagga ttgcagattg cgccgaatac aaatttttct tttccaactg ctttaaatgg
     4081 tcaaccgcta accccaggtg aatatcacct tacaatgact gttttaggaa atgaaaatga
     4141 aaatggaaaa ttttcgagaa aaaaaggaaa tactacgatc aactatacca atcaatgggt
     4201 atttgaaaaa gattttacaa tagacggaaa agtagccaag gaattgaaca cgacggatgt
     4261 tacgattaaa aaagacaata attggattta tatcttagta gcttcacttt tcttattgtt
     4321 tattttatta tgcatacttt tgtttgtttt gagaaaaaaa gaagagaaag aaaagtaagg
     4381 ttaagataac aaatagtaaa tttttattat tgtaggatgg tt
//
LOCUS       DAPCMT010000175         4402 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPCMT010000175 DAPCMT010000000
VERSION     DAPCMT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.179"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            141..416
                     /gene="rpsP"
                     /locus_tag="RD743_002621"
     CDS             141..416
                     /gene="rpsP"
                     /locus_tag="RD743_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDU0564398.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            428..679
                     /locus_tag="RD743_002622"
     CDS             428..679
                     /locus_tag="RD743_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDU0564399.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            804..1595
                     /locus_tag="RD743_002623"
     CDS             804..1595
                     /locus_tag="RD743_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564400.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1765..2286
                     /gene="rimM"
                     /locus_tag="RD743_002624"
     CDS             1765..2286
                     /gene="rimM"
                     /locus_tag="RD743_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDU0564401.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2276..3037
                     /gene="trmD"
                     /locus_tag="RD743_002625"
     CDS             2276..3037
                     /gene="trmD"
                     /locus_tag="RD743_002625"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDU0564402.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3173..3520
                     /gene="rplS"
                     /locus_tag="RD743_002626"
     CDS             3173..3520
                     /gene="rplS"
                     /locus_tag="RD743_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDU0564403.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(3652..>4402)
                     /locus_tag="RD743_002627"
     CDS             complement(3652..>4402)
                     /locus_tag="RD743_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0564404.1"
                     /translation="DDLSNKYVSVIKSKLNLLFSYAMKKGYVEKNPINEVVIDYKRES
                     KTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDVHITN
                     NNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQF
                     LFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDR
                     VGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         1472 a          778 c          863 g         1289 t
ORIGIN      
        1 aacaaaaaag aagtctaaaa tcttctattt tctaaaaaaa atcgaattta atcaagctgt
       61 aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac
      121 aatggaggtg gaaataaata atggcagtta aaatccgttt aaaacgtatg ggttctaaaa
      181 agagtccgtt ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat ggacgtttca
      241 tcgaaactgt aggtacttac aatcctttga aagaccctgc agaagtagtt ttaaaagaag
      301 acttagttct tgactggtta tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc
      361 tttcaaaaga aggcgttatg caaaaacacc acgaagctaa attctcaaag aaataaggtg
      421 accggatatg aaagatttaa gcgacttagt cttaacaatc gttcgtccgc tagtcactta
      481 tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt acaatctaac
      541 agtggctcct gaagatattg gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat
      601 ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat
      661 cgatgataag gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc agcttttttg
      721 ttttttttat taattgaaga aatgattatt tggcaaaaca agcaagaaag cgtatcctta
      781 taggaggagg gagtgaacag ccaatgagaa agaaaatgtt atttattcca tttgctctat
      841 tgttaagcgc ttgtagttcg gttactcaag aatcagacaa tacacatcta tcaagcagta
      901 tatctgaaac tgttaccagt ccagaaaaaa gtagtaccaa aacggcttcc accactgaac
      961 aaacaatggc tacctcaaat aatgagaaaa agactgcttt agaccagttg aaagaacaac
     1021 agccaaatgt tcccatgcct ctagatgtac cagtctcttc aggctatctc aatatcgcag
     1081 cgacacatac aaagcaaggt tattctattt tatattacag aacagatcgt cccctcgggt
     1141 tgaatgctga cgaattgaat caagaaacgc ctattgcaac ctatctatat caatatgggt
     1201 ttgcttctag tcaagaaaca atccaagttt tgcagccatt tgagatcgac acaaatggtc
     1261 agcaagtgga cttaggttct cgaatcacag ggtatcagca aggtgcagca ggttcaagtt
     1321 ttcttgaatg gcaagaagga aactggcgta ttcgaatccg tgggaataat atagaaggcc
     1381 aagatccctt actattagct aaagaaatcg tagcttattt ggaagaaaac agtctaccag
     1441 cacctgaaca atttggtaaa attactgttg atatgggtga tacgacgaat cgagccgtag
     1501 aagtaagctg gcaagaaccg aaaaatgcct atacgatcac tcatcaagat cctatgtctg
     1561 cgttgaagat ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat
     1621 catttaactc caagtcacag gaagtaatca aaaatagcac gccgcatttt tgcataattt
     1681 tggcttaata ccttaaaaga aaactagaat tgcctcttca cacatgctaa aataaaacag
     1741 acaaaaaaga aatggggata taagttgact gaatacttga atgtaggaaa aatcgtaaac
     1801 acacaaggta tcaaaggcga agtccgtgtt atctcaacaa cggatttccc agaagaacgt
     1861 tacaaaaaag gaacggtgct aacactattc caagacggaa aagcacctgt ggaattaaca
     1921 gtcaaaagtc atcgcaaaca taaaaatttt gacttattga gttttgaagg acatccttct
     1981 atcaatgatg tggaaaaata tcgggatggc atattaaaag tgtcaaaaga caactctgta
     2041 gatctagctg aaaatgaatt ctattaccat gagatcatcg gtctgcatgt attagaagat
     2101 ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc
     2161 gttcaacgtc cgaaaaaagc tgatatattg cttccatata tcgcttctgt cgtcaaagaa
     2221 gtcgatttag aagctggagt ggtccgtgta gaaattccag aaggactgat tgacgatgaa
     2281 gattgatgta ttgactttat ttccaagaat gtttgaaggt cctatggggg aatccatcat
     2341 cggtaaggct gtagataaag gattattaga tatcaatatc tctaacttcc gcgattattc
     2401 agataacaaa catcagacag tagatgatta tccatacggc ggtggagcag gtatgctgct
     2461 caaagtccag cccatctatg ataacatcca agccattgaa aaagctgcac cggatgtcaa
     2521 aaaaagagta attcttcttg acccagctgg taaacgattc gatcagaaaa tggcagaaga
     2581 attttcacaa gaagaacact tagtattcat ttgtggtcat tatgagggat atgatgaacg
     2641 tatccgcaca ttagtgacag atgaagtttc cttaggagat tatgtcctta ctgggggaga
     2701 attaggtgcg atggtcatga tcgatgcaac agttcggctt ttgccagatg tgttagggaa
     2761 tcagacttca gctcaaacgg attcttattc aaccggttta ttggaacatc cccaatatac
     2821 tcgcccggca gaatataaag ggatgaaagt accagaagta ttgacaaatg gaaatcataa
     2881 attgattgaa gaatggcaat taaaagaatc actaagaagg acctatcaac gtcggcctga
     2941 tcttttggaa acacttgaat ggacacctca aatgacgaaa tttttagaag aaatcaagaa
     3001 agaagaacaa gaaaaaacag cagatgaagc caagtgactt tttctaaaat cttctttaca
     3061 gtaggttgat aatatgttag tattttatgg tgagtgcaaa agcacttaac tattacgata
     3121 ttccgctgtg agaaaagctc ataagaatat ttggaataag gagaagaaaa ccatgaatcc
     3181 attaatcgaa gaattaacaa aagaacaatt acgttctgac atcccagcgt tccgccctgg
     3241 tgacactgtg cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta tccagttatt
     3301 tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc gaaacttata cagtacgtaa
     3361 agtttctaac ggagtgggcg ttgaacgtac attcccattg catacaccac gtgttgcaaa
     3421 aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt
     3481 acacggaaaa gctgctcgta tcaaagaaat ccgtcgttaa tcatcgttat cagactgaga
     3541 aagaaccctt ggtattcaag ggttcttttt ttgttctctg tttttttata ttaacgattt
     3601 ctatttggga catttttgat taataaaaag gggcaaaaaa ggggcaacaa gttacagttt
     3661 ttctatatct tctttcaatt tttcttttac tccctttgtc acatgcaaat aaatcttttc
     3721 agtgatatca ctattttcgt gcccaacgcg atcctgaata gcatacagag gagttcctag
     3781 ttccgctaat tttgaaatat gggtatgtct aaaaatatgt gaactaagtt ttttatcaat
     3841 cttcatgtca gctttatgat ttctcaaata ggtgttaatt gctgttagtt ggaaaggagt
     3901 tcctttcgtt gtttgaaata gaaattgacc attcggattt aattctagaa gctcattgta
     3961 aatagctatc gctttttttg gtaaatcaat ttctcgcatt ccagcagcag tttttgtaga
     4021 atcagatttt ttcatatcag ctattgaacg ttctctatac atcatcgtcc catttataac
     4081 tactgatgca ttattattgg taatgtgtac atcgtcttta cttaatgcga ttgcttctcc
     4141 aggcctcatt ccagtcaaat atagccactg gaaaaggaga gaatatcttt tattgtgtga
     4201 agttgtgaaa tttactagtc ttttatattc atcatcttct aaaaacttat ctttaatttt
     4261 tatcgttttt gactctcgtt tataatcaat aactacttcg ttgataggat ttttttctac
     4321 atagcctttt ttcatagcgt atgaaaaaag aagattcaat ttggatttaa ttacactgac
     4381 gtacttattt gacaagtcat ct
//
LOCUS       DAPCMT010000176         4391 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPCMT010000176 DAPCMT010000000
VERSION     DAPCMT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.180"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            12..902
                     /locus_tag="RD743_002628"
     CDS             12..902
                     /locus_tag="RD743_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDU0564405.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(975..1799)
                     /gene="nadE"
                     /locus_tag="RD743_002629"
     CDS             complement(975..1799)
                     /gene="nadE"
                     /locus_tag="RD743_002629"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDU0564406.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1811..3283)
                     /locus_tag="RD743_002630"
     CDS             complement(1811..3283)
                     /locus_tag="RD743_002630"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDU0564407.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3920..4282)
                     /locus_tag="RD743_002631"
     CDS             complement(3920..4282)
                     /locus_tag="RD743_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDU0564408.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
BASE COUNT         1279 a          885 c          759 g         1468 t
ORIGIN      
        1 aggtgatata tatgcaacta caaataaacg aagaatcctt accggtatat gaagcattag
       61 ctagtaaaac aaggatcaag atcatccagc tactttccaa aaaaaagatg aatgtcaaag
      121 atttggcaaa agaacttgga gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag
      181 aggaagcaaa catcatcaaa acagaaaaag taggacaaca aaaaatttcg agccttcgtg
      241 tcgataaaat cgatattagt tttccagaaa aaatattcaa tgcttttgat acgaaagaaa
      301 catcgatacc tattggtcac tatacaaatt atgcaattga accaacatgc ggtttagcta
      361 cgatccatga ttttatcggg aaagtggatg aaccacgcta ttttatggat cctcgaagga
      421 tggatgcccg cattctctgg tttaccagcg gatttgtcga gtatcaggca cctaattttt
      481 taaatacaga ggatacattg gagatgttgg aagtttctgt ggaaatttct tctgaatttc
      541 ctttttcaaa tgacaattgg ccttcagata tcactttcag cctaaatggt gtggagctag
      601 gaacatggac gagccctgga gattttgctg atatccgcgg taaatatacg cctgattggt
      661 atccagataa tcttaatcaa tacggcttgc tgaagacgat ccgaattacc aaacacttga
      721 cgaatatgaa tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag
      781 acacttggca tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg ggctgtactt
      841 tatttggtaa aggattcggt aattacgatc aggatatcaa attaaaagtt tactacagct
      901 gatatttttc ttttctattt tataaaacaa aaacagccaa tccctatgaa gggacggctg
      961 tttttgcttt attgttattt ccagaaatca tccaaaacgg aaattggcaa atgtcgttta
     1021 tgttgtgtct tgttccacca tgcttcgatt ttttcttgtg cttgagcggg aacttctttt
     1081 ccttctagat aatcatcaat ttcattatat gtgacaccta gtgcgacttc atctgcaatc
     1141 attggtttcc catcttcaag atctgcagtc ggaatttttg tgtataattt ttccggtgca
     1201 ttcaatgctt gaagcaattg tttcccttgt cgtttgtcca aacggaataa tggcaaaatg
     1261 tcggctccac catcaccaaa tttcgtgaag aatccagtaa tgttttctgc agcatgatct
     1321 gtccctaata cggctcctgc tctttctcca gctacggcat attgcgtaat catacgttga
     1381 cgtgctttga tatttccttt gttaaaatcg ctgattccca cacctgcttc gcttaatact
     1441 tgtacttgtg catcaacagc tggtttgata tttactcgca gactaacatc gggctggata
     1501 aagtcaagag cagcttttgc atcttcttca tctgcttgtt caccataagg aagacgaaca
     1561 gcgataaatt gatagttttc atcttttgtt tcttcacgca tttcttccat cgttagttgc
     1621 gccaaacggc ctgctaaagt agaatcttgg ccgccgctga tacctagtac aaaagtttta
     1681 agaaacggat attttttcaa atatgctttc ataaagtcaa tacttttacg tatttcttct
     1741 tttggatcaa ttgttggttt gacacctaat tcattgataa tttctgtctg caggctcatt
     1801 tattctcctc ctatgcttct gtatgaagtt ctgctacgct ttttttcatt cgttccatga
     1861 tagccatttt atggttccag cattcagtag acagatcgac tgggtatttt tgcggattca
     1921 agtctcgttt atactcttcc catagtgaat ctagattttc ttttgtatat tctttgattt
     1981 cgtttagtgc aggtaattcg taaactcttt ttccttcgat aaagatatct tgtaaaactg
     2041 ggcgagctgt aaagtctcga acagttttat tgataaacgt atgcactgga tggaacatga
     2101 agatagcttc ttcttctcga gggtcttcat cccataatgt cacgtagtct ccttcagatt
     2161 tcccatcaaa atttcgagtg atgcgccaca cttgtttttt ccctggagta gttacttttt
     2221 ctgcattgct tgataacttg atcgtatcca tcatttttcc ttcatcatct tcgatcgata
     2281 ctagcttgaa tactgcacca agggctggct gatcataggc tgtgatcaat tttgtcccaa
     2341 caccccatac atcaatttta gctttttgca tctttaaatt caaaatcgtt gcttcatcca
     2401 agtcattcga agcatagatt tttgcttccg taaatccggc tgcatctaat tgttcccgaa
     2461 cacgcttaga tatataggcc atatctccac tgtcgatgcg tacgccttgg aagttgattt
     2521 tgtcacctaa ttctttcgcc acgcgaatcg cacttggcac acctgatttc aacgtatcat
     2581 aagtgtcgac taagaacacg cagtctttat gggttttagc gtaggccata aatgcttcat
     2641 aatcatttcc ataagattgg actaacgaat gagcatgggt cccgctagct ggtatcccaa
     2701 atatcttccc agcacgtaca ttgcttgttg catctgctcc gccgatatat gccgctctag
     2761 ttccccagac tgctgcatcc agttcttgtg cccgtctagt tccaaattcc aatagtggat
     2821 cttctccgat gacagatttg atacgagctg cttttgtcgc gatcaacgtt tggaaattga
     2881 ccatattcaa cagtgctgtt tctactaatt ggcagtgcgc taaaggccct tccacttgga
     2941 tcaatggttc atttgcaaaa actagttctc cttcacgagc agaacgaact gttgctttaa
     3001 attcaaaatt ttgtaaatac tccaaaaaac cttcaggata gacttctaat ccacgcaagt
     3061 aagcaatatc ggaatcattg aacgtcagat tctctaaata attgactaac cgttccaatc
     3121 ctgcaaaaac cgcgtatccg tggttaaaag gcatttctcg aaagtaacat tcaaaaacag
     3181 catgtaaatc agctcgacct aattcccagt atgtctgcat catattgatt tgatatagat
     3241 ccgtatgcag cgtcaaactg tcgtctgggt aagtattatt catcttgtat tactcctctt
     3301 ggcaagtttt catttcctac ccattataac aaaaaaaatt aataggaaaa gaaatcttcc
     3361 attttttctg aaaaagcaca tgttttacgg atatttcttg ctaaaaatga actccatctg
     3421 ctaaaactat cctttcttat tggtatttaa cctaaacgag aaagaatact tattttattc
     3481 aatgtatccg agacacgaag aaattagcgt tttctatttt tcttacttga taaaataagt
     3541 agattttcct actacaatcg tagccgttaa gataccgatg attgaacaaa tataaccaaa
     3601 tgtctctaca acataatcaa tggcaaaagt ttttgaagca ataatacgtc tttccttttt
     3661 tgcttcttat ccccaaagaa acggatataa cttattatgt attgaatttt tttggaaagg
     3721 cgaattatga caagatatac cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt
     3781 ttaaaaagat acaaagtaag aaagcagctg tcaatagaat ggaaacaaaa atggcgagac
     3841 atcaatcagt ctcgccttgt tttttattgt atacattaga tatggagcag gttaccataa
     3901 agatgattat tagtcgttat taaatagaat ccttcgtatt tttttgttct atcactacaa
     3961 tatctttatt tggacgtttg aattgtattc cggcacgttc tagctcctgt ttcactccta
     4021 catggacttt gacatcttct cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt
     4081 cttgccttgt gatttttttc ttcgtattga tcatcacgac ttgcttttta tcatatgtgt
     4141 ctaccaatag cagttgtgtt cgatctaacg aaaataattt gtaataatta atagccgtat
     4201 tcccaaaaaa gaattccact actttaggat aaaatgtctt aatatttaaa ttactattca
     4261 caggtgtttc gattagcttc atctcattcc tcttttctat atagtcgatt tagatccaat
     4321 cttaagttat gttaccatct ttcctagcat taaggaatcg gttacaattg aacctttaaa
     4381 gacaaaagga t
//
LOCUS       DAPCMT010000177         4313 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPCMT010000177 DAPCMT010000000
VERSION     DAPCMT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.181"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..281)
                     /locus_tag="RD743_002632"
     CDS             complement(<1..281)
                     /locus_tag="RD743_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDU0564409.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="RD743_002633"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="RD743_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="RD743_002634"
     CDS             complement(2359..2721)
                     /locus_tag="RD743_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564410.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="RD743_002635"
     CDS             complement(2725..3063)
                     /locus_tag="RD743_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564411.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="RD743_002636"
     CDS             complement(3042..3461)
                     /locus_tag="RD743_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564412.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="RD743_002637"
     CDS             complement(3454..3687)
                     /locus_tag="RD743_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564413.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4313)
                     /locus_tag="RD743_002638"
     CDS             complement(3684..>4313)
                     /locus_tag="RD743_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDU0564414.1"
                     /translation="ISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRV
                     VDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLEL
                     QLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKK
                     KYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1221 a          959 c          618 g         1515 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aat
//
LOCUS       DAPCMT010000178         4272 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPCMT010000178 DAPCMT010000000
VERSION     DAPCMT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.182"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            537..1505
                     /locus_tag="RD743_002639"
     CDS             537..1505
                     /locus_tag="RD743_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0564415.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1516..2331
                     /locus_tag="RD743_002640"
     CDS             1516..2331
                     /locus_tag="RD743_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDU0564416.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2399..3109
                     /locus_tag="RD743_002641"
     CDS             2399..3109
                     /locus_tag="RD743_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0564417.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3145..>4272
                     /locus_tag="RD743_002642"
     CDS             3145..>4272
                     /locus_tag="RD743_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDU0564418.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1433 a          638 c          901 g         1300 t
ORIGIN      
        1 attatgagac aaaacaaaat gacctctttt atttaagaaa ggaggtcatt ttttgtatca
       61 taaattgtat taaaatcaat gtatcacttt ttaatcagtt ttaagaccat aaattgcgta
      121 ctaaaagaaa ttctgcaatt caaaataaag cattttaagc cattttcaaa tttaagtgca
      181 cccatctacc cgtcaaatca ctttattttc cactccagta gtttcctaat tcaactgtgt
      241 gcaaaaacga tttcttctaa acaaatcaag acaaacttat aaaccttcat cccagcacca
      301 taattattta aatcataggc atttttcgaa attagtatat gtactcaagt ttcactgcag
      361 attatttata ttttttagag aaataaattt ttcctattga caaacacaaa gaaagcggat
      421 acaatgcaat tatgtcacat gacgttatat gacgttatgt gatatcttta catttaatca
      481 gttactttta ttttggataa aattaacatt tttaaaattt taaggaggaa atgttgatgg
      541 atgtagcaaa aacggttaac gcaattgtag aaaaaaatga tggagagatt tctggaattt
      601 atttggtggc ttgtggcggt tcgctggtag atatgtatgt ctcagattat tttcttaaag
      661 ccgaagcatc gaagtgtatt acaggcttat atacagcgaa cgaatttaac catgtaaccc
      721 caaagcaatt agatgataaa tctgttgtca ttctatgttc tcatggagga aatacagcag
      781 aaacggtaaa agcaggagaa gtcgctcaag ctagaggagc tgtgactgtt ggtctgactc
      841 ataataaaac tgcggattta ttaaaggttt ctgatcatag tttcctttac gaatggggag
      901 atgatgcaaa aatagtcaat aatcctatgg ccatcattct tgatttaact gtatctttgg
      961 tcaatgttac cgaaacattt gaacattacg gtaaattcag agaaggcatg aaggttatcg
     1021 ataccgtaat tgaaaatggc attaagcagg tgcaggatcg tattaaagtg tttgctgata
     1081 agtacaaaga tgaaacaatg ttttatatct taggtagtgg accatcatat ggacatgctt
     1141 atggtttttc aatttgctct ttgatggaaa tgcaatggct tgattcaact tctgttcatt
     1201 ctggagaatt cttccatgga ccttttgaag taacagacaa agatacgaat tttattctct
     1261 tgatgagcat tggacgcact cgtgctttag atgaacgggc aaaacgattc cttgataaat
     1321 atgcagaaag agtagaaatc gttgatgcaa aggaactagg attggatatg atatcagatg
     1381 aagtttctga attcttcaat cccattttat tctacagtat tctttcagaa tatcgttcag
     1441 ctttagcaga tgtacgcaat catgatttag atgttcgtcg ctatatgggg aaagtggagt
     1501 actaggagga ttaaaatgaa ggtattaggc ttaggtgata acgtcgtcga taaatatgaa
     1561 aatctgggaa ttatgtatcc tggaggaaat tcattgaatt ttgcagtttt tgcaaaaaaa
     1621 ctgggacatg aaagtagctt tatgggagtt tttggctcag atgaggaggc agctcatgtt
     1681 ttatcagcaa taaaagaaat aggactggac aacagtcact ctcgctttga aaaaggcgag
     1741 aatggctgtg ccagagtgaa gattgatgag ggcgaccgga ttttccttgg cagcaattcg
     1801 ggaggtgtta ctcgtgaata cccgattcag cttactgaag aagacagaga atatttaaac
     1861 cagtttgaat tgattcatat gggattatat agccatgtca atcatctatt agaagagtta
     1921 caaaatgttt ctggaaagat ttcttatgat ttttctgacg actttacgga agaagaggtt
     1981 cagcgggcaa ttgataaagt tgattttggc ttcttttcta tagaagactt ttccgatgaa
     2041 gaagtaaagc agtttttgat gaagcatttt tgtgagcgaa atgatgtatt gattgcaacc
     2101 cgaggagaaa aaacggctat tggttatgat ggagcaaagt tttatgaagt gaaccccgtg
     2161 ctagaagaac cagttgatac aatggcagcg ggagattctt tcttaacagc tttcctactt
     2221 tattttctcg aaggggacga tatagtcacc gcgatgaaaa aggggaatga atttgctggg
     2281 aagtcttgtc tggttgacgg ttcgttcggc tttggtattc attacgattg agcttgtttt
     2341 aaattcctgc atttgataac ctaaagaaag aaatcccggt aaagtggagg ttaaaaaaat
     2401 gctggataca aatgcacaat taccattgta cttacaatta aaagaaatat taaaaaataa
     2461 aatcaataca ggggagtatc ccgagaatac gaaaattccc gctgaatcag agctgataga
     2521 aagttatgag gtcagccgaa tcacagtgag aaaagcagta gaagaactgg tagaagaagg
     2581 gtacctactt aaaaaacaag ggaaaggaac ttttgtcaac cggcataaag tttttcgtaa
     2641 aattgaatat attatgggtt tcagtgagag ttgtaaatct aatggtttta caccgactag
     2701 cattgttttg gaacgcaaga tcattccagc aactgaaggg atggcaaaaa aactcgatat
     2761 cgctcaaggt gatgatgttt tatatatcca gagaaagcga atggcggata acatccccat
     2821 tatgttagaa aacaattatt tttcttatga aagatatagt ttcttaatgg aggaagagct
     2881 gactgattct ttgtatgaag tacttaaagt ccaaggtacc ttagccatca atcctgggga
     2941 aactgcactt gaaattgtca cagctgatga ccaaattgcc aaaataatgg aagtagctgt
     3001 tgggacgccc tttttcttta tggatacttt aatcaacgat caaaacaaca taccaattca
     3061 tgtaggtaat caatattacc tgggagaagt atatcgtttt tacttatgat ttcctgtttg
     3121 gatttcaagg agtagatttt atttatgaag caaatattgc ggtcaaaaat gatttttgat
     3181 ggagagcagc cacctttcaa aggttatatt gtaatcaatg aagggatcat tgaaaaggtg
     3241 gaaaacaact ggaattacag tgatctatta aatgaaaaca cggaattaat tgcttatgat
     3301 gatcaattcg ttatgccagc aattcatgat aatcatgtat ttttcagtgg ttatttggct
     3361 atgaatgctg gcatagattt aagcgataca tcttcagcta acgaggctgt agagcgtatt
     3421 aaagctgtaa tgaaccataa aaaagcagat gaaccaattt atgctcatgg ctgggatgct
     3481 gccctgtgga aaactgaccc atcagaagag ctattgaatg aaataaacca ctttggtcca
     3541 atcacagcga tcgacaaaaa tcgcagccat tgttggatga atgctattgc taagcagact
     3601 tatggtttta cagaaaaaga aaccagtgct gaaagtcgag tattattaat taaggaaatg
     3661 ctagcaaaca aaaagctttt agcagaggtt tacgctgaat ttgaagcatt actgctttca
     3721 aaaggtgtta tatcaatgaa agaaatcgtt tttgatgacg cagaatttct ttctaatata
     3781 cctcagagga aaatacttag taacctatat gtgcaggcag tccaagaacc tctacagcat
     3841 gaacttctat atgagtataa ggaaaaagtt ttccctgaaa aggtccgctt tggtggagtg
     3901 aagatcatgg tagatggtgt tgttgcggat agaagtggag atatttacgg tgtttatcag
     3961 tcaggagtag cttcacccga gattgactat gaggcaatta aagcagaagt tgctgcattc
     4021 aatgaagtag agattccttg ctgtttgaca acggaaggag ataaagcggg ttataaagct
     4081 gcgtcaattc ttgtcgaatt tggaaagcgg ttacctgaag gcgtttttaa ttcgattagt
     4141 gatttggaaa tggttactca aaagacagca gaaaaaatga acgaaggaca agtagtagca
     4201 gaggtatatc cacaaattct cggcttgaat caaacgttag aagaggctta catgtcaact
     4261 gttataaaaa at
//
LOCUS       DAPCMT010000179         4248 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPCMT010000179 DAPCMT010000000
VERSION     DAPCMT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.183"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(27..1793)
                     /locus_tag="RD743_002643"
     CDS             complement(27..1793)
                     /locus_tag="RD743_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0564419.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1753..3507)
                     /locus_tag="RD743_002644"
     CDS             complement(1753..3507)
                     /locus_tag="RD743_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0564420.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3617..4186)
                     /locus_tag="RD743_002645"
     CDS             complement(3617..4186)
                     /locus_tag="RD743_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDU0564421.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1430 a          807 c          596 g         1415 t
ORIGIN      
        1 tgtaggttaa tttaaagcct atttcatcat cgtgattgag cttctttttc atagacgaat
       61 ttctcagata gtttagtttt ctttatgaga tttttgtata tttctgacct tcgatacaac
      121 tcattatgag taccttgatc aactagtttt ccttgatcca tcactacaat tttatttacc
      181 ttttcaatag acttcaagcg atgcgatata attagtactg tcttatcttg tattaaacga
      241 tttaaacttt cctgaatacg tttttcattt tccgcatcta aactggcagt aatttcatct
      301 aaaattaaaa ttggcgcatt tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct
      361 cctccagaaa gtgaacttcc gttttctccg accaatgtat gataaccgtc aggtagtttt
      421 tcaataaagt cttcgcagtt agctaaacga gctgcttttt tcacttcctc atctgacgct
      481 tgactatttc caatacgtat attttccaaa atacttgtat taaacaaagt tacctcttga
      541 aaaacaatag cgattttgct aaacaaagaa gctggagata cacgtttaat atcataccca
      601 tcaattaaaa tacagccttc atcataatca tacagtctag acactaattt caagatacta
      661 gttttcccgg aaccactagc acctactaac gcagtcactt ctccttgttt agccgtaaaa
      721 gagatatgat ctaaaattgg ggtattgtta tcatatgaga aagagacatc tcgcaactca
      781 atgtcaaaag atttcagagg actatctgat ccctcttgga tactggtttc cttcatagct
      841 ctaattcgct gtattttagg agctaaataa aatatttcaa gcactgcttc tttcatagaa
      901 tcgaaggaat cctttatttt tattgcggct agtaagtaac caacgacgta gagtatagtc
      961 acttcacccg ttaacagtaa gtgaacacct actagaatga ctactgctaa actaacatat
     1021 gagaaaatag aggataaggc catgactgaa aaagtactta attctacctt taaatgaatc
     1081 ctttcactct cttccatttt tttgaaaagt ctgtcttgaa cttttttaga tagattaaag
     1141 ctattaatct cttgctgcaa ttctatagtt tcttgaaatt cttccgagtt ttctctcaaa
     1201 gtatcgtaat atttagtatt ggctttcgtt tgggatttct ttgataacaa gattaagaca
     1261 aaactaaata acgttggcaa aataacagct aatcccattt tgacattacc aaccaaaagc
     1321 atcactgaaa taaaagggaa aaacagagcc ataccgccgg ctttaggtat tgcatgactc
     1381 atcgcatgct caataccttc aacgtcagac atgatagttt gagataaatc tgataaatta
     1441 tgttttgaaa aataggataa tggtaaattt gacaaatcgt ctgcaatttg cactcttaaa
     1501 tgcgcacttt ctttataagt actattatat aagttttcat actccctatc taacaaccaa
     1561 tacatgaaaa gcaaagtaac aaacgatacg gcaaaataaa gccaacgggg ctttgtattt
     1621 tctaaaacta attcgtctat cagcatcatt ataatgaatg ctggtaccat attaatgcag
     1681 tatacaaaaa aactagaaat agaagcttta gttaaagctt ctgcgccctg atcacttaaa
     1741 gcaaatttgt ttttataaaa acttttcatt gtcaagcctc caatcatttg cattctcata
     1801 attagtgacc aattgtttat acacttgatg tttagccatt aattcattat gatttccacg
     1861 ttctactatt ttcccttctg acaagacaag aatttcatct acagatttaa tgctggataa
     1921 tcgatgagct atcataataa ctgttttttc tttcatcaga tgttgaaaag ccttttgtag
     1981 ttcatactca ttatctggat caatagatgc actggcttca tccataataa caatctttgc
     2041 atcttttaaa attgctctgg caatagctat acgttgtttt tctcctcctg acagataaac
     2101 tcctttacta ccaattaagg tatgctctct ttcgggaaat ttttctaaaa tcgattcgca
     2161 accggctaat cttagcgcgt caaatacttt acttgctggc gcttgaggat ttgctataga
     2221 aacgttatcg taaatactct gattgaatag tttcgtatct tgaaagacaa aagcaattgt
     2281 gttaataatt gtgtcttttg aatagtgatt taaaggctta ccaccaattt taatctgtcc
     2341 cttatccaca ttgtagaaac ctgaaattag tttagctatc gtactctttc cgcttcctga
     2401 ttttcctact aatgcataaa ttcttttttg ttctaaaact aagttgaaat tttctaaaac
     2461 acactcttct ttgtatgaaa agctgacatt ttcaaatgca atgtcacaat ttttaatctg
     2521 tgtctcgttc ccatatttta gttggtcttg cttcatttct aaataaaggg agtctagtgt
     2581 ttcgactgca tatcttgctt caaacaagtg catacttaca tacattattc tcatgaaaga
     2641 aacaaaaata attccactta aaaaaaacaa catcaacaag tcaacagcta atatcttggg
     2701 agtggtaaga gaagttataa aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa
     2761 aaatagccat tgaaaaagaa caaaaggtgt tttcccactc aaagaatact tatatgcaaa
     2821 cttagcataa tcgttaattg ctttattcaa ggctttaaat gatgttactt tagcaccaaa
     2881 tatctttata acttgaattc cgcggatata ttctaccgtt tcagagctta acacatctaa
     2941 cgattcttga tatttttgaa taaaatttgt attctctgtc attttcttta acaagaatac
     3001 accaattact aacatggcga ttagaaccaa tccaatttta aagttaatga aaaagcctaa
     3061 ggcaactgat aaaacaggtg ttactatggc ttgagcacta tctggaatca agtgagcaat
     3121 tgccatatgt gttttagccg cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt
     3181 taaatcaaaa aaacgaaaac ttgcttctgt taaaccgcta attccctttt ttctcaaatt
     3241 agtctctaag cgaaacccaa ggatatgaga aaataagcca gaaaaaatat aaaataaagc
     3301 tcctaaagtt aaccaagcaa cagtttgaac cgcataactc ttagctaaat attcatctcc
     3361 tacaataatt agagactgaa gaaatttgta aatactgtaa tatccataga ctgttaatac
     3421 gacagaaaaa tcagaaaata aagtggctaa ccatccgtag tatttcattt tagggacata
     3481 actaaataat tttttgtata ctttcatttt ttcctccccc gcattcaatt tcttctctta
     3541 ttcgtttaca actgttaatg ctttccgtaa tagcgtaaat tagagatgta caaacagata
     3601 aaaggtaaac tacgttctat tttttgtatt tttttaagac ttttcttcca atcaatacac
     3661 caatcataga taaacttgct acaactaaca ctgaaatgat tccccatata cctaccgcat
     3721 agtggatcat tgcatccatt gttgcagcag attgtccttt atctatccaa tcttgacgaa
     3781 aacgatctgt aaagaaccat ataggaatcc actgtccggc agcactgcct aattgcatta
     3841 gagcccaaga cgcaacttga ggaattgctc gatcatatcc aaatacagat agaataatat
     3901 cagctaagat cgccatacta atcgaactga taaaccaagg taagtacgcc ccgcccatta
     3961 acgtaaaaaa taacaataaa acaattgtgt aaatagtaaa aattcctttt ctagggtagt
     4021 tatagctaat ataaacaaat atgattccag ataataatcc aaaaatcccg ggggatatgc
     4081 tgtgaccaaa cgccccacca atactactaa ccattattga aaaaaagtaa attactaggg
     4141 aaataactgt agctaaacca ataaaaatat aatcttttgt tttcatatat attctcctca
     4201 attctatttc gttatgtcat taaaaatctg ttctatcaaa gttgaatc
//
LOCUS       DAPCMT010000180         4235 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPCMT010000180 DAPCMT010000000
VERSION     DAPCMT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.184"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(204..650)
                     /locus_tag="RD743_002646"
     CDS             complement(204..650)
                     /locus_tag="RD743_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDU0564422.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            1245..1595
                     /locus_tag="RD743_002647"
     CDS             1245..1595
                     /locus_tag="RD743_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564423.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1800..2648
                     /locus_tag="RD743_002648"
     CDS             1800..2648
                     /locus_tag="RD743_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HDU0564424.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            2695..2889
                     /locus_tag="RD743_002649"
     CDS             2695..2889
                     /locus_tag="RD743_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDU0564425.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            2892..4094
                     /locus_tag="RD743_002650"
     CDS             2892..4094
                     /locus_tag="RD743_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0564426.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
BASE COUNT         1483 a          789 c          725 g         1238 t
ORIGIN      
        1 caaaaaaact ctataactaa gaaaaatcac aaaaaagagt acattttcta gtgaaactgc
       61 accctttttt gctttgttta cgtacaaaaa atctcttgag tacttaactt ctacagtata
      121 atcaacaacc cctttataga ttactgatgt tatgctgaaa aaaatttttt caccaacttg
      181 taatctaagc cacttttctt attttacttc tcaaaccaat cataaaattc atctggtgtt
      241 ttctcccgat agagtttgat taacgctggt cgcatcgatt gaaatatttc gactaattct
      301 tccccaccaa atcgagtgga aaaatgctta tcagtaaata tcattgataa agaagaaaga
      361 aacagctccg tatcttcatc tgtaccaaga atattgatca actttccaat caaataggaa
      421 tcattaaatg tcaggtcttg tttcacatca aaggttttta ttgattcttt caccattttc
      481 gcaatgcgat ctttctctcc atacttgatc tcaccactca tctcgatata gtcaatgatc
      541 ccagatgaaa gaatcgtgac agcttcttct tttgcttttg atgatagttt gtttagcgtt
      601 ttggattctt caagtacctc tttcgtaatc tgtacgacat agtccttcat gatttcactc
      661 gaatattcta caaaacattc ttggataaat tcctccaaag gatagcctag ctcattctcc
      721 caagcaggat ttttgaactc gcccgtataa aattcttgct ttattttctc aatgatttca
      781 ggataatcgt ccactatttt tttctgacca atgagaaaca aatactctcg gatatattct
      841 tccacatgcc caaaataaaa ccagccgaca ggttttccgc tgatactaga taattggttg
      901 atcacactct taggagccaa cgcatagcct tgaacgtagg aactgatcgt cggctttttt
      961 aaacctaatc gattccctaa ttctgtaaaa gagagagaca ttccgtcttt tatttctttg
     1021 attcgttggc ccaccttcac tttattaggt tgtactaaac caaacatatt caaattcctc
     1081 cttatcattt ttttcattat aacactcgta taaaaaatcg tatatatttt tttagaaaat
     1141 ctcttgacta cgtataagtt ctcgtatata atacaagtcg tagatggcca tctatatttt
     1201 ttaatcaaat cgtataaact ttcgtataaa aaaggagaat tgtgatgcca ataaaaatta
     1261 ccgatctcag tttaccaaaa gaaacactcg aaaaagaata ccaagtcgtc aatgtcactc
     1321 gctggcaaaa ggagggcgaa attctaggtt ggagctacga atgtatccta ccaaaacttc
     1381 gctacgaaaa aatcagtgtc aaagtcaaat ctgaacatcc tgtgatcacg ccggaagaac
     1441 ttacccaaca aggtttagcc ataattacat ttaccaattt ggtgattgta ccttggggac
     1501 gctcgaatgg acaatttgtc agttatgggt tatcggcaac cgctgatacg gcaactttga
     1561 ttcccagaaa acaaggtacc actacacaaa actaactata ctatggagtg ttgggtggtt
     1621 aggacgaaac ggcggggcag cgcccacaga gccgtttctg tccgttaaga ccgcccaaca
     1681 ccccataggc tagaagggct gctacgcagg ccttccccct taacttgatt tatagatcaa
     1741 catcaggaga aaatgactgg ttctttgaca atcgaatacg caacaaggag tgatgatttt
     1801 tgcaagctta taatcagaaa atcattgaaa ctcccacgta tattgaaatt tatgattatg
     1861 aagcaatcat tactcccaaa cgtaaaacga tcgaaaacga agtaaatctg gaacgaaaac
     1921 aaaaattagc ttggatttat gaaaatgata ttactcttga aaaaagaaaa aactatgatg
     1981 aattatccgc tcaaggacaa tatgacagtt taaaacgcaa acagaaacat tatcaaagta
     2041 tgcgatttga gattgcccgc cttgtagaca cgaattttga caatcaaacg aaatttttaa
     2101 ctctcacttt caaagaaaac attcaagaca ttgagtatgc aaataatgaa tttaaaaaat
     2161 ttatcaaacg actgaattat gacctctata aaactaaaaa agcaaattta aagtatctcg
     2221 ccacgtggga aaagcagcaa cgcggtgcaa tccactacca tattatcttg tttgatttcc
     2281 cctttatctc atttaaccgg ttaactacga tttggggtca gggtttcgtt aagatcaaca
     2341 agatcgatgt agacagtatc gaaaatcgcg ggcgctatat cagcaaatat ttcgataaag
     2401 agctggaatt gaaagagcat aagaaaaaag cttttttcaa gtcccggaat ctcaaactac
     2461 caactgtcac caaaaagatg gtaaagaaac cctatgattc cacgaatcaa gatatactat
     2521 atacaagcga atatatagtt agacgtccat ttttttacaa tgctttagct ttaaatgagt
     2581 tgggaagtcc tgaacaagtg atggattttc aagaaagtac cgttcattat acaaaaataa
     2641 agaaataaca ctattaccca atgatagtgt tttctttgaa agtgagttta tacaatgaaa
     2701 aatacaattt gtcgaaaaga tttagaggct ctaggattta aaacacatca agctaccaca
     2761 ataatccgac aagcaaaaac aagaatggta aatcagggat atccatatta taataataaa
     2821 agagttgggg tagtaccccg acaagtaatt gaagaaatta taggtgttcc tctttcacag
     2881 gaggaataac tatgaaaaca aaataccctt gtgtgtatca agataaaaat ggtcgctttt
     2941 cttatctagt tgagttagga acagatcaag tgactggaaa gaggatccag aagaaaggaa
     3001 caaaagattc tctaggaaaa cgattctcat cagctcgtga agcacacaaa gaagtcatgc
     3061 gtataaaaaa tgaatacatg gttgtaaatg gatttgcgaa ttatgattta acttacaaag
     3121 aatttatgga gcagtcttac gttccacact acaaagctag cgtacaaaaa agtacttgga
     3181 gttctcgtga gtgtggattg attcagatta ctgaattttt cggtgataag aagcttcgtg
     3241 aaatcaatgt gcgagattgt gaaaattatc gaatttggtt attgaacgat agcggatact
     3301 cacaagcata cagttcactt atgtacggta tgtttcgtaa atcattagac tatgcggtca
     3361 ccctgcaatt tctgaatgag aatatttcca aaagaacaaa agcaattcca aaaggaaaat
     3421 caatcgttcc ttactggaca aaagaagaat ttgaagaagt tttggcaaaa atttataaag
     3481 atgatttcta cgaacatatg tgttttttaa tgatctggct atattacatg actggtgttc
     3541 gtgtaagcga aggattagca cttaattgga atgacgtgga cttcaaacgt aaaaagttga
     3601 gggttcatca caccttggaa atgacaaatc aaaaagattt tattcggaaa ccttacacaa
     3661 aaactgaaag cggcttgcgg atcatttctc tggacgatga tacaattgaa gtgctaaaaa
     3721 agtggaaagt tattcaaaaa aatcatggtg tagaacaatt tattcttagc tacactgacc
     3781 tcccactcta tcgttcaact gttcaacgaa ttattcagcg ttatgccaaa ctagctaagg
     3841 tacccctaat acaaggaaaa ggattacgtc attctcatgt tagctaccta atcaatgagt
     3901 ttaacgcaga cattctggtc gtgtcgcaac ggttaggcca ttctagccct gaaatcactt
     3961 taaagcacta cgctcatcta tggagccgta atgatgaagg aattgcagaa caaatgacag
     4021 gaaacattaa atttacctgt gcaacaaaat ctaaagttgc aaactttaat ggaaatcaag
     4081 cagtaaaaat ctaaccctac catttcccta ccaaaatgag gaataggctg aaaaagcact
     4141 gatataatag cttctttacg aggctactca tattgagtgg gaataagacg taaatataat
     4201 atggatgggg aatgccgata aatcaacgtt tttca
//
LOCUS       DAPCMT010000181         4201 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPCMT010000181 DAPCMT010000000
VERSION     DAPCMT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.185"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(1..352)
                     /locus_tag="RD743_002651"
                     /pseudo
     CDS             complement(1..352)
                     /locus_tag="RD743_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1160)
                     /locus_tag="RD743_002652"
     CDS             complement(612..1160)
                     /locus_tag="RD743_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564427.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1161..2018)
                     /locus_tag="RD743_002653"
     CDS             complement(1161..2018)
                     /locus_tag="RD743_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDU0564428.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2304..2590)
                     /locus_tag="RD743_002654"
                     /pseudo
     CDS             complement(2304..2590)
                     /locus_tag="RD743_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2580..2909)
                     /locus_tag="RD743_002655"
     CDS             complement(2580..2909)
                     /locus_tag="RD743_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDU0564429.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2986..3258)
                     /locus_tag="RD743_002656"
     CDS             complement(2986..3258)
                     /locus_tag="RD743_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDU0564430.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3258..3521)
                     /locus_tag="RD743_002657"
     CDS             complement(3258..3521)
                     /locus_tag="RD743_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDU0564431.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3627..4043)
                     /locus_tag="RD743_002658"
     CDS             complement(3627..4043)
                     /locus_tag="RD743_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564432.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4049..>4201)
                     /locus_tag="RD743_002659"
     CDS             complement(4049..>4201)
                     /locus_tag="RD743_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDU0564433.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1224 a          851 c          533 g         1593 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac ttatttgaga gtcatctaat tggctcataa
      241 tcctttcttt ttggttagta tctgcagtta cttgtttagt atgagccatg aacccaaaca
      301 tactcacccc aacaaccaat cctaaatata ttttcttttt aaactttttc atctacatct
      361 cctcctaata gttttatttt agagttagct ctattactta tttactaact caacaacaga
      421 ttatcataca cttgttaatg aaatcacaat atctcattaa aacacaccct aaaatcataa
      481 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
      541 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      601 ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag tgagtcttcg
      661 gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc taattctgga
      721 acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt aaacaaatct
      781 tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt tctaagcaat
      841 tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt cgcatttaac
      901 aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt tctgattggt
      961 gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga agtttttatt
     1021 tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc ttgctgcttt
     1081 ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc aaaaatatca
     1141 agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg ttcttccaat
     1201 tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt taagagacct
     1261 ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg cgcatttttg
     1321 agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt atacatttcc
     1381 tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac atataaattt
     1441 gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc atttctcatc
     1501 gattttactt gggtctgtaa gacgacgatt gcccaatccg caatgtacat cgcggattta
     1561 ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc atcaaataat
     1621 ggttctagct tttctttcat atatttgaat tgggataatg aatcattata ttggtctttg
     1681 atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag gttattgatt
     1741 actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt cacagtattt
     1801 tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt catatgcgtt
     1861 aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa ggttcttttt
     1921 ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt acctacgcca
     1981 cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct tttcttttag
     2041 caatattcat ttgatactat tataacatat ttatttttag ttactaccaa aaaaaagata
     2101 tatatgtact accttataat taataagtaa aaggtagtac ataaataagg aaaagctgaa
     2161 atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc gagaagcaga
     2221 gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa gaagccgtcc
     2281 ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata aaagttttca
     2341 tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta agctactcta
     2401 tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc cccagttttt
     2461 tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa aaatttttct
     2521 ttcaatggct tctcttttag ctttttaaat acttaatgac atgttttgaa ggaacgattt
     2581 cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta
     2641 tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca
     2701 agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa
     2761 tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt
     2821 gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa
     2881 ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt
     2941 acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta
     3001 atgcgtcctt gctgaataaa tttcaaccac gtgttcatct tcatctacct tataaacaac
     3061 ccggtgttgt ctattcaatc ttctagaata cctaccttca tgtgtaggtt ttaacttctc
     3121 aaatgattgg gtagggagat agggatcttc ctttaaagtc tgaataacct tctcaaattg
     3181 aggtttcaaa ttagactgtt tgattttctt taaatcagct ttagccgagt ttttaatcat
     3241 cacagaatag ttattcatta taagttatcc caatcaattt catcaacatt agtaaatcca
     3301 ctatcatctt tttctcgctt acgaacaaca cccaaagtgc ctatttgctc aagtaatagc
     3361 gtttctttga ttgctttcca atcatccaaa ccaataagca ccgcattatt ttcactgcgc
     3421 tcactaatga tttcaatttc cgtatgattc tcatttacat ctttcaacaa cttataaaaa
     3481 tttttccgtg ctgagctagg atttaagact gtttttgcca tatctactcc ccctctaatt
     3541 tatatctcca ttctaacgta cttaattaag tacgtaaatt cgattgtttt gtttaaattg
     3601 gagtaccaaa acagcattgt aaagtgttat ccgtttcctt tatttttatt aacaagtttt
     3661 atcacaggct tcatatattt gattataact tcatcttcta cgtagatatc tgttatcatt
     3721 acgacatctg tactatttcg attgagtact ttcacttgat ctccagcatt gattttccct
     3781 ctaaatcttt taggaatgaa ccaactatac atatgattag catcaccaaa atgtgctttt
     3841 atcgcattct caggatataa cttattcaat agttcattct ttttaatttt caaaacttca
     3901 aatctatact tatcatcatc tgctaatcta aataaatctt ctaaacgcat gttttctaac
     3961 tgtaagtttt gaaaatcttc cggtataaat tttcggttct gttgcgaagt ttgaaaatca
     4021 aacgcgccct ctctgaactc caaatatctt aggctgttat tcccattaat accttgattt
     4081 cagtagacac cgaaaagctg aagagcgttc catttcttcg gttcttttta tatattcctc
     4141 gaatggtctc catgccctta atcgtggaag aggctgtacg gagactttga taaaatttat
     4201 t
//
LOCUS       DAPCMT010000182         4167 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPCMT010000182 DAPCMT010000000
VERSION     DAPCMT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.186"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            59..1360
                     /locus_tag="RD743_002660"
     CDS             59..1360
                     /locus_tag="RD743_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0564434.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1387..1647
                     /locus_tag="RD743_002661"
     CDS             1387..1647
                     /locus_tag="RD743_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDU0564435.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2646..3881)
                     /locus_tag="RD743_002662"
     CDS             complement(2646..3881)
                     /locus_tag="RD743_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDU0564436.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1201 a          668 c          867 g         1431 t
ORIGIN      
        1 attctttgcg ttgtcatgca atgaatggtt catattttat tgaaaaggag tggttttagt
       61 ggaaaagctg atgaaatggg tcgaagagaa acttgtccca ccaatggcaa aaattggtac
      121 acagcgtcat ttattagcaa ttcgtaatgg ggttgtttca acattgtcat tgattttgat
      181 tgggacattt tttatggtct ttatcaattt accgtttcct ggatggaatg aatttattgc
      241 cccttattcg gcaacgattg ttttaccctt taggatgacg atgggattga tggctattta
      301 tgcaactttt gttatgggtt cggatctagc taaatcatat ggattagata gtgtgacagg
      361 tggtatatta tctttaggaa cgttctttat gttacaagtt cctgtaaacg ttttaactcc
      421 tgaagaggca cctcttggct gggtcttacc tatgagttct ttgggagcat ctgggatgtt
      481 tgcggggatc ttatctatga tttttgcagt ggaagtatat cgtttcttta aaaatcgaaa
      541 tattactatc aaaatgccag aacaagtgcc acctgctgtt gcccgtagtt ttgaggcatt
      601 gattccaggt gcagtcattt taacaattac ttggctgatt cgaagcgtcg cgggctttga
      661 tgtaaacgct gccttattat cgttattcga gcctttaacc aatattttag ggaataattt
      721 gcttggtgtt ttactaccaa tgtttttgat ccatttgttg tggtcatttg gtatccatgg
      781 catgagtatt gtaggggctg ttgttcgtcc aatgtggctg atcatgttgg atgagaatgc
      841 aaaggcattg gcagatggta cagctgccac ccaattaccc tatatcgctc ctgaacagtt
      901 ttatcaatgg acggtaacaa ttggtggtgc aggagcaacg atcgtggtat ctgttttgtt
      961 tatattcttc tgtaaatcaa aattcttgaa agaggttggt cgtttttcta ttattcctgg
     1021 tatcttcaat atcaatgagc ccataatttt tggagctcct atgatgttga atccttacat
     1081 gttcatcccg ttcaacttag ttcctttagt tttgactatt atcagttatt ttgcagtcaa
     1141 attagaaatg gtcaatagat ttactgtttt gccaacatgg acattaccag ctccaatcgg
     1201 cggttatctt tcagcaggaa atgactggcg tgttgttatc ttgatcatga tcaacacatt
     1261 gatagcattt attatctatt atccatttgt aaaagcttat gataaaaaga tgctgtcaga
     1321 tgaacaaagt cgtggagaaa acgaagtcca gactgtctaa gtttggtttt tagaaggtgg
     1381 gaaacaatgt attggctgat tttatttttt gtttttatct ttttattaac ggctagtcac
     1441 cttatattga atatgctagc tacatatcat attcaaatca atcgatggat ttgggcactg
     1501 gcatcatttt tgatcgtgat tcttccaaag atcattgtac cgcatatgaa tgttttgttt
     1561 tcatggggga catatgtact ttgtggaatt tttgctatca attttatgat cgagcagcat
     1621 agatggtttg tcacatcgaa gctttaaatg ttctaaaagt ggttgtaaca gaagtggtta
     1681 gcttcaagaa ataagaagaa atttacgaaa attgcttctt aaatttttgt gaaatgttgg
     1741 cttatttttc gtttggttga acttattacc gcagatgcaa gcttttgacc accgtttatt
     1801 cggaatatgg gactgtgaca aggcttttgt cacagccctt ttttacataa agattattat
     1861 tgttttaact gttttggcag aaagaaggag gaaaaaatgg aaattttatc agcaatggat
     1921 ttagaattag tagatctact accaaagtga tcaagtcaaa tatagatttg ggagcttaca
     1981 accatatagt aggggctgca gtatggtgta tgaatggtaa tatgtatgat gtgagtgttt
     2041 attcaaatca tgaaacgtgt atagaaataa ttgtgctggg aatagcaatc agtcatggtg
     2101 aacgtgactt ttagtgtatc gttgctgacg acatagaaca ttctgacatg atctactcaa
     2161 tatgtgggga ttgctggcag tttatatagg agtatgcatc ggattgcgag atcattgtct
     2221 caacagaaga gggaggttga ttatcaaaga taaagtggta aatcctacat ttgatttagt
     2281 aattgttgag gggactagcg gcacaaatct ctacaaagcg atgcttctag atctatatga
     2341 cacattttaa gaaaatgtat ttgcctaatt attatggtct ttcttttgaa gaaacgttga
     2401 gatgatccaa ccagagaaag gtgtcaaaga atttgactaa gaataaatgg cagacgctac
     2461 ctatgctcat ttggatagcg tcagatcttt tattttaagg atgattttgt gttttttaga
     2521 caccgtaatt taagtaactc taacttttca atccactatt tacttttttt gtattttcac
     2581 tgtctgggtc tcgaatagcg aaattactga tgagtctcta ttaaaaaagc tttttctact
     2641 tgaaactata ttgggtggtt gctcaggaag gtttcgattt cttgaattgc ttgttgtgga
     2701 ccatttattg gaaaaacggc ttgcagcgtg cgtgcctgct gacgaatctt gggtgaagaa
     2761 acttcgcgca gagcaatctg cagttttctt tcagtcactg gttttgtgag ggcgattgcg
     2821 tttccgtatt ttacacaata gtcaatatta cagctttgct caaattgcat ccccacaccg
     2881 ataaacggtt tgccggattc acaggcagtt tggacggtcc cttccccgcc gtggataaaa
     2941 gaaaaatcaa tgaattcatg caaaagatga ctagacagat attctgttat taaaagatct
     3001 tctgagtcag attgaatgtc atcaagcaga tagtcaatag gtgcaataac cgctagatcc
     3061 ggttgttttt gtaagaagaa caaaattttg cgtaacatac gcggattgcc tgaactaccc
     3121 attgcgaaat agataatacg ttttctttct tgttgctttc gctgtataaa agttagcact
     3181 tcttcaggca aggaagtact gagcttagca aaaattggac caacgattcg ataattttcc
     3241 ggcagctgtt cttcttccaa aaatagttta ggcgttgtga tgagatttag atcagcatcc
     3301 atcatttgta aacttgtttt tggtaaagga agcatttctt tttgtgcagc gtgtttgaag
     3361 gcattgggga tccatcgggt atttactata cgcttttttg tccagtgcca aaggggattg
     3421 cttaaagggc cgaagaaacg taactcgtgg ggcatcagcg catgctctga ttgaaaaaaa
     3481 ggttcactca aagcataggg tttaacatat actaaaggaa tctttttatt gcgggccgat
     3541 aaaaaaattg tcacattcga acctgtcaca atcagtcgag gctcaaactt agcaatcaga
     3601 ctctgttcac tagtgatacg agaggcaacc atttctttgg taaaagggtt tttaagtgta
     3661 cgcaactggt caaattttat gatttgttct tcttgttttt tcgtaaggat cggcgctagt
     3721 aaatgatact caaagccact ttctgtgatc aaaggagcaa aagttttaga aaagccaaag
     3781 aatacacagt aataacggtc tttcatagct ttggcaacct caatcattcg tgttgtctca
     3841 gctaaattga atactgcagg agcaaaaaga attgtcttca tgaaatcctc cttattggaa
     3901 tgagtctatc ccttcgtttc ttactagtat actaaaaaac acagcgttcg agtagcctat
     3961 gtatattttg aatattaaac tgtctctcgc tgttttcaca acttctaaat ttaaattttg
     4021 taatcaagtt gaaactccaa ttttcaaaaa aacacgcaac aattttttga tcgatttata
     4081 tgttgtgcca gataaatacg agaagttgtg tcattaaaaa taaacatgag aacataaaaa
     4141 aataaaataa ttatattaat tgatagg
//
LOCUS       DAPCMT010000183         4071 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPCMT010000183 DAPCMT010000000
VERSION     DAPCMT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.187"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            33..149
                     /locus_tag="RD743_002663"
     CDS             33..149
                     /locus_tag="RD743_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDU0564437.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            165..968
                     /locus_tag="RD743_002664"
     CDS             165..968
                     /locus_tag="RD743_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDU0564438.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            1203..1439
                     /locus_tag="RD743_002665"
     CDS             1203..1439
                     /locus_tag="RD743_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564439.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1446..3398
                     /locus_tag="RD743_002666"
     CDS             1446..3398
                     /locus_tag="RD743_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDU0564440.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3470..3967)
                     /gene="dfrG"
                     /locus_tag="RD743_002667"
     CDS             complement(3470..3967)
                     /gene="dfrG"
                     /locus_tag="RD743_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDU0564441.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1434 a          660 c          740 g         1237 t
ORIGIN      
        1 ttttgtaccc agtttaagaa tacctttatc atgtgattct aaagtatccg gagaatatct
       61 gtatgctttg tatgcctatg gttatgcata aaaatcccag tgataaaagt atttatcact
      121 gggattttta tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata
      181 ttggagtttt agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata
      241 acagtggagc gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata
      301 cgcttttaga acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg
      361 aaaatacgaa ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat
      421 atcgttcatt atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac
      481 aagcacaaac tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt
      541 ttatcaataa gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag
      601 agaaactttc tgccgaaatt gtaatcaaac agaaggtaga actgtatcct aatatgtgtg
      661 tgacgaactt taccgaatct gaacaatggg atacggtaat agagggaaac gatgaccttt
      721 tagagaaata tatgtccggt aaatcattag aagcattgga actcgaacaa gaggaaagca
      781 taagatttca gaattgttct ctgttccctc tttatcatgg aagtgcaaaa agtaatatag
      841 ggattgataa ccttatagaa gttattacta ataaatttta ttcatcaaca catcgaggtc
      901 cgtctgaact ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg
      961 catattaagt agttcagttt tggagtacaa aaagtgaaaa tgtaaaccta aatgagtatc
     1021 aaattgcaac tgaatttgat actcgttttt tattgttaaa tcaacgtttg ttgttaattt
     1081 acaccacaaa atgtaagtta aaatgtatat aaaaatgcga acgtgaaata attattgtag
     1141 gaatgcaaat tggtgtgtta aaatggttac aatttactaa gcaaaaaggg gtgcattttc
     1201 ctatgcaatt tcttgagcca aaaaataaga acgctaagtc tgtggattgg gaaatatcag
     1261 agcaagtaag ggtgatagtt aagcaatacg ctgaatatgc agagcgtact gaaagcgaag
     1321 cagtggatga attcttattg aacattttag acgataagaa gtttattgaa tggattgcca
     1381 acaagcgaag taataagcgg attgttgaaa aaatgggtat caaagataga gtgggttgat
     1441 agacaatgac caagcttaaa agattagcca cgaaggaaga tgaaattgta gatgttaaaa
     1501 ttccgatttc aaacgaagaa ttaaaagata gggctaaaca atatgattta ttaactccaa
     1561 aacgatttgc aacaaggtat aacaaaatgt tgttcttgcc aacgtctttt aaatggaacg
     1621 gttctgaata tcccattcaa tataattact gcatcaatcc gttttgttgt aattttggga
     1681 aagaacagca caaattcaaa gatgtaaaag ggaaaccaag tcgatataaa atgactggta
     1741 gttccaaaga taaaggtcat aaaggtatgt actgcaatga caatcctatt ggcagaggtg
     1801 tatcgcaaaa ctgtacagta acccctttat ctaattggtc tgttgttgaa gaaattaaac
     1861 gccttataga aatcaatagc atccaagatg ttgagccaga ttatcaattt cataaagaag
     1921 gctgctcgga agaagaatca accccattca atgagccgaa gcaattttac aaaaggggga
     1981 aaagtagggg caaatctcaa cgctatcaat gtaaggcgtg taagaagttc actaatgttc
     2041 taccaaaaag agaagaaacc actacttatc accaacaaaa gaacaccatt cttccaatgt
     2101 tcgctaagat ggttgttgga agggtttcag tcagtcgtac ttgtgatatt ttaggaattg
     2161 gagttggaac ctactatcat aagctggagt ggctgtatag gcgttgtttg gagtttttag
     2221 aacgatatga gacacaaccc cttcaaacaa agaaatttaa cgagatgtgg cttaatacgg
     2281 acaagatgca ctactaccta aataacgtcc gtaagaaagg acaaggttca aagaaatata
     2341 cggggtttga agaccttaat atgcaaacgt atatagttgt atctgctgag gtcttatcaa
     2401 gatatgtgtt ccgttctgat gttgcctatg attggaatat ttcaatggat gaattaaatg
     2461 aagatacaag aaaattcaaa gaagaccatc tcaatacttt tagtcgtaaa aatgataggt
     2521 tagattggtc ttattatcca caagaaccat ccgctaacga tagcgaaaat aggaatgctt
     2581 atcttcacga attagggaaa attactaata ggagtagatt tgtcgatggt ttaaatgttg
     2641 atgctcctta tactacaact gctcactatt ggttaattaa acaaatggtt aatgccgacg
     2701 aatggcgaat gattagtgat gatgacttct ctattagaaa cgccttttat agagtattca
     2761 cgaaagaatt aaggttgagc gatgcacatc attttatttg ccaagttaac aagacgaaaa
     2821 gcagaaaaca atgcttaaaa gaattcggac aagcaaaagc agaactgctc gattggggag
     2881 atatcagagg attcaaaaca aagtttctac ggactttggc atctcattat ctaactgaat
     2941 tactaacaag tcatcaattc cacgaagagg ctattagtaa agatggagaa agatatcgca
     3001 aatatgccga taaccctatc aaacatccat tagccactaa ggataaagga ttttattcag
     3061 ttgattgtag aacggattta tctgctctgg aaccaaacga aatcgcaaag atgctattaa
     3121 atgttaatga ccattcgacc aatagcttta ttcaacagat aagacgttat atatcatctc
     3181 ttgaaagacc attaacaaca gctcgtggtg ataagaagag ctatatttac gctaacttca
     3241 atccgaagta tgctcaattt gcgattacaa ttctaagaac ttactacaac ttttgtcgcc
     3301 cttttaaatc agcggataaa aaggttttga cacctgctca aagattaggg attacagata
     3361 aacaatttga ttggaaagat ataatttatt tcaaatgaca aaaagagctt ggagtaatcc
     3421 caagctcttg tgctttatct ttactgttct tcttcaatta acgcacccgt taactcaata
     3481 agttttttct ttcatataca tgaaaatagt agttatacgg atttttatca ttctttatcc
     3541 ctttttgggc aaatacctca ttccattcct cataattcac ttctggaaaa aaagtatctc
     3601 cttcgaattc atgatgtatt tttgtgatgt acattttctc aacataaggg aaaaacaaat
     3661 tataaatctg ttctcctccg aaaataaaaa tttcttcttc gtttttacat aactcaaaaa
     3721 catcttctat tgaatgaaca atttcacaac cattaaaggt aaacccctta tctctcgtca
     3781 gaataatatt tcttctgtca ggtaaggctc ttccgattga ttcaaggttc ttcctaccta
     3841 atattatcgg atgtcccttt gtagtatttt taacatattc ccagtccttg ggaatcctcc
     3901 aaggaatgtc attctctttg ccaatcactc tattcttatc catcgcagca atcaaagaaa
     3961 ctttcattta taacacagcc tttttaacta atttaccatc taatttctta tttctatcaa
     4021 aaaacctttt ctcattatcc tgcccgttag ttgaataagc aattttttct a
//
LOCUS       DAPCMT010000184         4064 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPCMT010000184 DAPCMT010000000
VERSION     DAPCMT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.188"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(174..1679)
                     /locus_tag="RD743_002668"
     CDS             complement(174..1679)
                     /locus_tag="RD743_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0564442.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1676..2362)
                     /locus_tag="RD743_002669"
     CDS             complement(1676..2362)
                     /locus_tag="RD743_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0564443.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2555..3976)
                     /gene="gndA"
                     /locus_tag="RD743_002670"
     CDS             complement(2555..3976)
                     /gene="gndA"
                     /locus_tag="RD743_002670"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDU0564444.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1117 a          858 c          694 g         1395 t
ORIGIN      
        1 gcattttatt gttttttcgt tcatctaaaa gatacttaca tttaattaac ggatgttatt
       61 ctatttaatt gttcgctagt ttttttataa tgacatccct gaatttctca cttgcttgta
      121 ttttgtacct tattctttgt tggatatata aaaaaacaag taaagcacat gctttacttg
      181 tcttctgcct ttgcgatagg aataaagatt ctaaaaattg tcccttgccc tactacactt
      241 tctgctaaga tacgtccttt atagttttca accagttgtt tagcgataga caaccccaga
      301 ccatttccac ccttcgttct agctcttgct ttatccactc gataaaaacg attgaagatc
      361 ttattaaggt cttctttggg tattccttcg ccaaagtcct gaatcgcgat ctcaaattca
      421 ttcagagtgg aagaaataga gatatggact tcttttcgtg tagttgaata ctttactgcg
      481 ttatccaata tgataatgat taattgttca aagtgattac gataaatttg cagggtcact
      541 tcttttgtca aatcatcatc taaagtgatc acatagtctg gatataatat cttgaaatta
      601 ttataaactt gataggtgac ttcttttcct ttagttgttt cattcgcata atgaacatct
      661 acttgttctg ctcttgataa gtctagcatc tcttgaacta agcttttcat acggctgatt
      721 tcttgcatgc tggcgcttaa tgattcttct aatatctcag gatcatcttt cccccaacga
      781 tttagcagac taagatgccc ttcaataatt gcaacaggtg tccttaactc atgagatacg
      841 tcttccacga attgctcttg ttgttcaata tacaaacgca tacggtcaag catctcattg
      901 aatatttcag ctaaatctgc caattcatca tttgtgtcga tttcaggcat gtggatatct
      961 gattgcggat ctttacgtat agtatccatt gtatctctga gtaccttgag tggttttaag
     1021 aaataagaag ataaaaagaa acctaaaata ctacttaaga tcaaagaaac aacttctaaa
     1081 acaataagag tcaataatag cttatttcga atatcataga aagaagataa ttcatagaag
     1141 ctttgcgcat agccgatctt ttctcttgta cctttagaat atataggctg gatagataaa
     1201 aagccagttt ttccttctag tgtaacgatc gttggaactt tacgtgtagt ctgaatcagg
     1261 tttcgactgt tttcatgggt tttaaaaatc aagttctcgt ccaaatcata aacataaaga
     1321 tccattgatg gctgccctag ctcagctata aaggaatcga tcttcattaa gtttccttcg
     1381 accagtgaag tatgatctac gacctcatct ccgtcatttg tcgctttcgt cagattacga
     1441 tacgtattta ctaatgtcag ttcttcatcc gagttagcca aacgagaagc tacttctgaa
     1501 atcgtacgct ctacattgtt tctttctttt gccacaatca gattgataga tgatttatag
     1561 gtaatgacag cgaaaattgt aaacacaacg aatatgaaga aagaactcgc aaaggcccac
     1621 ttgattgtca aggagggccc tttcagttct cttttttccg attttttcgg ctttatcacg
     1681 agcgcatcac ataacctgtt ccgcgtacag tttggatata gctttcctcc ccgggaacgt
     1741 caattttgtt gcgaagataa cgaatatata cgtccactac gtttgtttca acttctgttt
     1801 cgtatcccca cactttattt agtaaaacgt cacgtgctaa taccacgttc acgttttcca
     1861 ttaaagtaag cagcaactcg tattctcttt ttgttagttc aattacttca tttccgcgac
     1921 gtacgacacg gttttctttt tcgatagtca aatcgcgata agtaatagta gtttgtttag
     1981 ccacattttt atcgccttca atatcgattc gacgcaacaa tgcacgaaga cgtgctaata
     2041 attcttcgat agcaaatggt ttgacgatgt agtcatctgc tccgtggtca agtccagata
     2101 cacgatcgat cacagaatct cttgcagtca tcataatgat tggtgtattt ttgacttggc
     2161 gtacacgtct gcagacttct aaaccgttca attctgggag catcagatcc aacaatatgg
     2221 catcccactc attatttaat gccgcatcta aaccagttcg gccgttgtag tggacttctg
     2281 cgttataccc ttcatgcttc aactctagct cgacaaatcg tgccaagttc ttttcatctt
     2341 caatgattag aatattgctc atttattatt ttatcctttc tattctactg gctttttaaa
     2401 aactctatca ttataccgat aatatgctta aaaatctata gtcattttat catggatagc
     2461 aattttaaaa aagagctgaa gtctactcat aacaatcgac ttcagctctt tcgtcctctt
     2521 tttattttct catttcctaa cagccgaaag actattattc ttcatggtac catgagtaat
     2581 ggaagattcc ttctttgtcg acacgctcat aggtatgtgc gccaaagtaa tcgcgttggg
     2641 cttggattat atttgcaggc agacgttctg ctctgtatga atcgaagtaa gcgatggctg
     2701 atgagaaagt cggtacaggt acgccagctt ggacagccaa ggcaacgacg tctctgactg
     2761 attgctgata ttttttcgtg atttcaacga agtaatcatc tagcaataaa ttttcgatct
     2821 caggattttt gtcataggca tccgtaattt tttgcaagaa tcgagctcgg atgatacatc
     2881 cagcgcgcca gatttttgcg atttcaccaa atggcaagtc ccaatcaaat tcttttgaag
     2941 cgacacgtaa ttgagcaaat ccttgggcat aactcatgat tttactgaaa tacaacgctt
     3001 ctcggatttt ttcgaccaat tcttttttgt ctcccgtgaa ctcaaaatcg ttcgtacgag
     3061 acaaaatctt gcttgcttgt acgcgttctt ctttataagc agaaatatag cgggcaaata
     3121 cagattcagt gatcaatgga agtggcacac ctaagtcaag cgcgctttgg cttgtccatt
     3181 tacctgttcc tttattccca gctgcatcaa ggatcacatc tactactggt ttacctgttc
     3241 cttcatcgtc tttacgtgtc agaatgtcag cagtgatttc tatcaaatag ctgtctaatt
     3301 cgccttgatt ccattcttta aagatatcag ccatttcttc tactgaaagg tttaaaatat
     3361 tcttcattaa gtcataagat tcagcaatca gttgcatatc tccatactca ataccgttgt
     3421 gtaccatttt cacataatgt ccggctccat ttgggccaat atatgtgaca catggtgctc
     3481 cgtcttctgc ttttgcagaa atttgctcta agattggtgc aactagcgca taggcttctt
     3541 tttgtccacc aggcatgatt gaaggtcctt ttaaagctcc ttcttcacca ccggaaacgc
     3601 ctgtaccgat aaagttgatg ccagagttgg ctaactcttc attacgacgc atcgtatctt
     3661 taaagaatgt atttcctccg tcaatcaaaa tatcgccttt atcaaggtga ggaagtaact
     3721 cttggattgt tgcatctgtt gccggtcctg ctttgaccat tagcaagatc cgacgtggtt
     3781 tctcgataga ttctacaaat tcttcgattg tatatgttgc tttgaaattt ttatctgggt
     3841 gttcttctac tacagcggtt gttttagccc ctgtacgatt gaataaagcc actgaatagc
     3901 cgcggctttc tatgttcaag gctagatttt tacccataac ggccataccg acaacgccga
     3961 tttgttgttt tgacatatga actcctccta catacgggat ttctccctaa tctcttacat
     4021 tatagcttaa cacggctaca ttgcaaacta aagaacaaca tatt
//
LOCUS       DAPCMT010000185         3952 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPCMT010000185 DAPCMT010000000
VERSION     DAPCMT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.189"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            22..696
                     /locus_tag="RD743_002671"
     CDS             22..696
                     /locus_tag="RD743_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDU0564445.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            689..853
                     /locus_tag="RD743_002672"
     CDS             689..853
                     /locus_tag="RD743_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564446.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            929..2071
                     /gene="dcm"
                     /locus_tag="RD743_002673"
                     /pseudo
     CDS             929..2071
                     /gene="dcm"
                     /locus_tag="RD743_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            2061..2351
                     /locus_tag="RD743_002674"
     CDS             2061..2351
                     /locus_tag="RD743_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564447.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2610..3794
                     /locus_tag="RD743_002675"
     CDS             2610..3794
                     /locus_tag="RD743_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDU0564448.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            3791..3928
                     /locus_tag="RD743_002676"
     CDS             3791..3928
                     /locus_tag="RD743_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDU0564449.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         1282 a          658 c          918 g         1094 t
ORIGIN      
        1 ggaagaaagg ggataattaa tatggaaagt aatgattctt atttagagta tgcaaaagca
       61 aataaggata agtttatttc ttatattatc aaagggaaat cccccgaagt tggacgagac
      121 gcagttttta tggcagggag tcctggggct ggaaaatcag aggttgccat tggattagct
      181 gagaattatg ataaccatgt gattattgat gcggattact ttcgcaccca gtttcctgat
      241 tataatggaa aaaactctag tctctttcaa aaagcatctt cttggttggt agagcaggca
      301 tttagatatg tcttagatca tggctattct tttattctgg atgcaacttt tgcaatatta
      361 agtgctgaaa aaaatatctc gcgggcgttg aaaaaacatt ttcgagtgac gattttctat
      421 gtttatcaag accctaaaat cgcttggaaa tttacacaag aacgtgaaat tgcagaggga
      481 cgtcatgtgc cgaaaaaggc atttattaac gcttttttca aatcaagaga gaacattgca
      541 aaagtaaaga gtaaacatcc agatgtattg cttcatatca taataaaaga ttatacgaat
      601 aatatttccg aagttcatta tgctacggat aatctagaat tatttttacc actagggtat
      661 agtaggaaag atttggaggt tcaattacat gactgagaaa gaacaagtaa cgaaaattgt
      721 aaagaaatac aataagagca ttgcggattt atcggaaaat gctactgcaa aagagttcaa
      781 aacggtgatt aaatatgtag ccgatcaggc caatgaaaaa caacggaaat tagttggatt
      841 aaataaaaaa tagtaggaca aattaataat gtatgagcca agagcaaaat gttcttggct
      901 tttttctttg caatgaatga ggtaatgtat gagattttta gatttattcg ccggtattgg
      961 tggctttcgt ttaggtatgg aacaggcgag ccatcattgt atcggttttt gtgaaattga
     1021 taaatttgct agacgtagtt ataaagcaat tcatgataca agtaaggagg tggaaatgca
     1081 tgacatcaca agtgtatcag atgagtttat tcaatccctc ggaccagtgg acatcctttg
     1141 cggcggattt ccgtgccaag ctttttcaat tgcgggaaag cggcaaagat tttccgatac
     1201 tcgaggtact ttattctttg aaatcgctcg gttcaccact cttctccgac ctaagctttt
     1261 attccttgag aacgtccgag gattgctcaa tcacgaagga ggggctacgt tcgagacgat
     1321 cctccgaacg ctggatggat tggggtatga cgtggagtgg taggtgctta actcaaaggc
     1381 ctacattccc caaagccggg agcggatatt ccttatcgga cattctcgag acgcatgtac
     1441 cgaacaagta tttcctatca ttggaccgtc ttcaacatct gatcaaaaca tcagaaattt
     1501 ggtaaatatc aatccttcca atcgaggaat gggtggacaa gtctatggtt cagatggcgt
     1561 agcgccaacc ttaacaggtg atgaaggaat caaaatcgca ctgccagcaa gggatggaat
     1621 ctctgtagca ggactattac cgggaaagtt tgaacaaggt aatcgagttt atgaagttac
     1681 tggaaccgcc cccaccttgt caacgaaaca aggtggtaca aaaatcatga ttcgaaaaaa
     1741 tgaaacagaa tatcaagagg tgaagccaga agacagtgtg aatctggctt ttcctaattc
     1801 cacaagtcgc cgaggtcgtc tgggaaaaca gagtgttcat accttactaa caggagacca
     1861 acaagcgatc gtcacggatc agtatcaaat tcggaagcta acacctagag agtgttggcg
     1921 acttcagggc tttcccgatt gggcttttga tcgagattct caagtcaatt cagatagtca
     1981 gttgtataag caagccggaa actctgtaac agttccggtc atttttgata ttgccagaag
     2041 attaaaagag ttggatgtac atggtttatg atgagttgat tggagaaatt tattgggtga
     2101 ttggaaagat tcagtcagac ccagaattag aagaacaatt gcgacggtta aaatttgata
     2161 ttcgcaagaa tggtgtcaaa gtcgctggcg atccatattt aatgaatgag gagacggatg
     2221 ctcgaattga aataaatcaa gtaattgcag aatttgagcg gattgcggac caagcaaaag
     2281 aaccagatat tcgtcaatac ttgttagaaa tgaaagcaga gttggaagta aatggtatta
     2341 cagatgagta acctaaacca acacaactga ctgttaggcg ctgaccgcgg aggattcgcg
     2401 aagcagaaat cctccgtggt gctgtgcctg ttcatggacg gcgttagccg accatgatgt
     2461 ctcacccccg tatctaacag gggggtacaa aaacacaaaa aataagtaag caaccattaa
     2521 gaatgcttgt tgtatcaaag atagtaagta atttgtatag gtgttaactc atttgaaaaa
     2581 gttaacacct tgaatttgga ggtgagaact tggcacaacg gaatttagat tatcggctat
     2641 taaaagaccg acgcaacgaa tacggcgttt cacaaaataa attggctacg gcttgtggac
     2701 ttagtcggcc gtacttaaat cagatagaaa atggtggcgt aaccgcatct ataaaaacca
     2761 tgaggaaaat ttttgaccaa ctggaaagct ttaatcccga tttgcctttg acgttactgt
     2821 ttgactatgt aaggattcgc tttcccacaa cggatgcacg aaaaattatt caagaaattc
     2881 tccatttgaa atttgagtat atgctccatg aagattatgc cttttactcc tatcaagaac
     2941 agtatgtcat gggtgatatt gtggtgatgt tgtctcatga agaagataaa ggtgtgcttt
     3001 tagaattaaa ggggcgaggt tgccgacaat ttgaaacttt tttactcgct caaaagcgta
     3061 gctggtacga ctttttcgag gattgtctaa aggccggtgg cgtgatgaaa cgcttggact
     3121 tggcaatcaa tgatcgagtg gggctattgg atatacctga gctaacgaaa aaatgtcaga
     3181 aggaagaatg tatctccttg tttcgtacct ttaaaagtta tcgttccggg gaacttttaa
     3241 aggctgatga aaaggacggc atgggcaata ccttatatat cggcagtctc aagagtgagg
     3301 tttatttctg tttgtatgaa aaagactatg aacaatatat caagttaggg attccattag
     3361 accaaactga gacgaaaaat cgttttgaga ttcgactgaa aaatgaccgt gcttttcatg
     3421 cgattcaaga tttgttaaaa ggtcgtagca ttgagagcac cacgttttcc attatcaatc
     3481 gttacttgcg atttgcggat aaagtagaag gtaaaaggcg aaccaactgg cccttgaatg
     3541 aacaatgggg gcgttttatt ggacggaatc ggaaagagat tcaattaact tccgagccaa
     3601 aaccgtatac cattgaacga accttgaatt ggctaggccg acaagtcgcc cctacgtgga
     3661 aaatggctaa ggagttggat cgattgaatc ggacaaccta tatccaggat atggtacaga
     3721 atgcacgctt atcagaccga cataaaaaga ttttggagca acaaagtatg gcaattgaaa
     3781 atttgattat atgaaaagag gaatcgtcat gggaattgta aaagatattt ttttggtatt
     3841 tggtggcagt atgcttggtg ttactgtgat gtgcttgatg aatgcaagtg cagcagcgga
     3901 tcgagcaatg gaaaagaaag gaaagtgaca agatgaattt cggacaaaat tt
//
LOCUS       DAPCMT010000186         3732 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPCMT010000186 DAPCMT010000000
VERSION     DAPCMT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.190"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            75..470
                     /locus_tag="RD743_002677"
                     /pseudo
     CDS             75..470
                     /locus_tag="RD743_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(465..683)
                     /locus_tag="RD743_002678"
     CDS             complement(465..683)
                     /locus_tag="RD743_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564450.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="RD743_002679"
     CDS             790..1104
                     /locus_tag="RD743_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564451.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="RD743_002680"
     CDS             1208..2386
                     /locus_tag="RD743_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0564452.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="RD743_002681"
     CDS             2789..3082
                     /locus_tag="RD743_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDU0564453.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1273 a          551 c          718 g         1190 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa ttaatcaagt
     3721 tttatctttt tt
//
LOCUS       DAPCMT010000187         3714 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPCMT010000187 DAPCMT010000000
VERSION     DAPCMT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.191"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="RD743_002682"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="RD743_002682"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDU0564454.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="RD743_002683"
     CDS             complement(2262..2513)
                     /locus_tag="RD743_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDU0564455.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="RD743_002684"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="RD743_002684"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDU0564456.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="RD743_002685"
     CDS             3463..3681
                     /locus_tag="RD743_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564457.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1204 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caag
//
LOCUS       DAPCMT010000188         3599 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPCMT010000188 DAPCMT010000000
VERSION     DAPCMT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.192"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            27..2024
                     /locus_tag="RD743_002686"
     CDS             27..2024
                     /locus_tag="RD743_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564458.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(2100..2435)
                     /locus_tag="RD743_002687"
     CDS             complement(2100..2435)
                     /locus_tag="RD743_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0564459.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            2562..2777
                     /locus_tag="RD743_002688"
     CDS             2562..2777
                     /locus_tag="RD743_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0564460.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            2814..3176
                     /locus_tag="RD743_002689"
     CDS             2814..3176
                     /locus_tag="RD743_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDU0564461.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            3222..3548
                     /locus_tag="RD743_002690"
     CDS             3222..3548
                     /locus_tag="RD743_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564462.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         1303 a          471 c          721 g         1104 t
ORIGIN      
        1 cagaatttta taaggaggaa acacaaatgg aaacatcaat gattaatatt ccaatttatg
       61 tagatcaaga aaaattatta gagaagccag ttacaaaaaa agaaaaaact gatgtaagag
      121 ataggtgttt agataactat agagaagtta caagagatga gtttgtagag atactaaatt
      181 catcacaatc atttattcca agcaagacaa agaataaagg caatgacaag gagagttttg
      241 tggaaaccag agtaattatt ctggatgtcg ataacactgt taaagatgag aaggggaagg
      301 tagtagattt gagtaaagat gatagtagat acttaagcgt agaaaaggtt ttatctatca
      361 atttagttca taattctgcg tttgcgatac aaaaaagtat tcggtactct gaaaatctag
      421 aaaaatacaa gattgtcttt ctattaaaag aagcaattac tgactatggt aaaatggttg
      481 ctgtttacga atacttgaaa aagaacatcc ccgggtgtga tgacaatgta aatgcttcga
      541 atagaatgtt ttttggagga tataagtcag atgcagtgat aataattaat gaagacaaca
      601 tgttagatat tactgagctt ccgattgatt ttaccatgac atgttctaaa aattgtcaaa
      661 gtgattcagg gagagagtat gaactagtca atgaaaccga ttttgttaag ttgataaaaa
      721 atgacgacaa agaagaaatg aaacagtggt ttaaagactg cctgtttgat tcctccgaca
      781 tgtcttttaa tgagatttat gaaagattgt taaccattga tatgaataaa ttacttaagt
      841 caagtaaaaa cttaagatgt ctgtttcaca acgatcataa tccctctgct agtattttta
      901 ccagtaaaac tgggcattcc attttttatt gtcattccag taattgtgat atcagtgaaa
      961 attttattgg agttgtgatg ctaattttaa agaaagacag tcgggtagaa acatttaatt
     1021 ggctattaga gaacctagac ttgtatccta cacattttcg aaaattgaaa gaggaatcaa
     1081 aagtactttt tgacactctt gaatcaatga aagacaaact tttcaggaca ataaggaatt
     1141 atctagagga aatgcgacgt gtctatgatg tcttgctttc tgatgttaat ttttttgatg
     1201 atagtaagga aagtgtgact tgtattttgt cgggggaaca attagctaaa aaaatgagtt
     1261 cttactatgg taaaacttat gacatagata aatgtaataa gttactatca cttatgactt
     1321 ttataggatt gataaaaaaa ttagatgatg aagatgttcc tttgaagatg ctcgaatacc
     1381 taaataggtt aaaggaagag aaagcgttag gaaactttaa tcataagaga agcaatgtct
     1441 atcgattaga cgtttttaat gcaacttctg tgattcaaaa attgaatgaa gatatccttc
     1501 cacttctgga aaaaattcat tttacatatc aatatttttc ttatgaatgg gttaagatat
     1561 gttttaacga aaaagaggct aaaagagtat ttcctcaaaa ttcccgatca gaactcagca
     1621 aggaaaaaca attgatttta gatgaagcac atgaagtttt acagggctta tcgtggcaag
     1681 ggacatatct aatgacagag aaagaactta ttaacactgt ttgtgaacgc ctacgtaatg
     1741 ttggggataa cagcgtgaaa aatgctttaa taaaaaacag aggctacttt gttaaacaag
     1801 gattcactct tgaacgagct tctaaggata ttaagaaact atacaatgtg aaagcaagta
     1861 gtagtttttt aatatattta gcgaaagggg cagggataga tgaaacaata aaagaattga
     1921 tattatcagg agctgggata gttcctctgt atcaatttac aagtgtaaca agagatgatg
     1981 gacggagagt aaggaagaga aaagacccaa ttgtattaaa ctagacaggt tatccatagc
     2041 tcttaatctt atttcatctc aatacaccca ccgttaaagt tcggtgggtt atttattttt
     2101 caatctttca atagctgatt gcgttcattt tcttgccttt ttaatagctt catatagtca
     2161 attgttcgaa ctgcatcttc agtgctaaga gaacgataga gagccaaaaa ctcttcctca
     2221 taatctacga caacattgtc tagaaaatgg cttacagggg ttcctagggc tctagaaaga
     2281 gctgttaagc tatcgaggtt aggctctgtt acagaatttt cccatcttgc tactgcctgt
     2341 tgtgtagaga agattttttc agctaattct ttttgtgtcc acccgagagc ttttcgcttt
     2401 tctctaatga cctttgcata ttgtttctgt ttcattaata cacccccatc tcagtatagt
     2461 acaacaagtt gttgtatttg taatggtatt tgaagttttt tatattttat gagttgaaat
     2521 acatcatatt gttgtatata ataaaacagg aggggagaat catgaaaaaa gtaagtaatc
     2581 tcagccgata cagaaaagaa aaaggacttt ctcaaacgga gttagcaaaa aaaatgaatg
     2641 ttacacagca atgtgtaagt tcgtggcaaa cagggagaac gattcctaaa ccctatcaaa
     2701 tgaagatgct ttctgagata ttaagtgtac caatgaatga gctgttttct gatgtattta
     2761 ataaagtaaa cagttaacta ataagcaatt gaatgatttt aggaggagaa gacatgtatt
     2821 gtagaaaatg tggcaaagaa ataacagatg aaagtaagta ttgccagttt tgtggagcac
     2881 tacaagaaac agatgagaca accggaacag acacagtaaa gaaaagtact gagcagacaa
     2941 ttaatgaaat gaaggaacaa atcgtacatg gacaagagac agggaagctg tatttgatta
     3001 ttggttggat ttctatggta gtatcgttag catttattcc tgttgtattt ggtgctgttg
     3061 ctgttattat gggctactta tatcgagaaa aagatgagaa aatgggtacg attttgatga
     3121 tagcaggggt agctggagca atctttggtg tacttttagg gatgtcaatg tactgataag
     3181 agggataaga gcaaattatt ttggagatag gagataaaaa gttgaataaa gtaaatatga
     3241 acaagaagtt tgtcattata ggtattgttg tcttggctat tataggtatt attttttcaa
     3301 aaatgacagg tacatctgga ttagagaagt catcaaaacc attggtaaca cagattttac
     3361 aagaacagta caacttaaat cgatcatgtg atgatgtgac gattactcat gaaaatggag
     3421 ataacacata ccgggctaaa gctatattag acaatggaag tgctatcaac ataaatattg
     3481 agtactatcc aaagaaagac cgtatatatg tggagatacc atatgctgag gtgttaatgt
     3541 taaattgatt taaatgtatt atgcataccg tccaaacagg aactcccaaa ggtgtaaat
//
LOCUS       DAPCMT010000189         3566 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPCMT010000189 DAPCMT010000000
VERSION     DAPCMT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.193"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..394
                     /locus_tag="RD743_002691"
     CDS             <1..394
                     /locus_tag="RD743_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564463.1"
                     /translation="LLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELYKEF
                     GDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNAI
                     SIKDLKELLNSKRNELKQIKETNSVELE"
     gene            408..1097
                     /locus_tag="RD743_002692"
     CDS             408..1097
                     /locus_tag="RD743_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564464.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1222..>3566
                     /locus_tag="RD743_002693"
     CDS             1222..>3566
                     /locus_tag="RD743_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDU0564465.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGAYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKL"
BASE COUNT         1208 a          469 c          751 g         1138 t
ORIGIN      
        1 tcttttgaat ttagattctg ttaattcaaa cgctcaaata aaagatcaaa gagtagctga
       61 acataataat aaaaaaatga gtttgaccaa aaatatctat tctatgtcag atgaagaatt
      121 gtataaagaa tttggtgatt tatccgggga acagttagaa gaaagtattc caggaattag
      181 tgactcagat aaaattgttg tcttgtctgc tggtgggcaa ttaatgggag atggcaaatc
      241 cgaattagtt agttataaac aaaatcacag acagtttatt gatgttaata ctgattccaa
      301 cgctatttcg attaaggatc taaaagagct tttaaatagt aaaagaaatg aattaaagca
      361 gataaaagaa actaattcag tggaattaga gtaaaggaag attcattatg aaaaagattt
      421 ttggtgtttc tgttgggcta cttatgatgt taacatttgg aaatgcagta tatgctgctg
      481 agaaagaagt tatttcagat ccagagcagt acgcctcacg atactttgct gattttgaag
      541 aagaagataa agtacttgca ttgccagaag gtggtttttt aaaaggggaa gctaaaattg
      601 tatctatgga taatccagat ataattcaag gttcatataa ttcagaaact gatcctgctt
      661 cagtttcagt gacagaagca aaaaaaattg ttgctgaaaa attcaaaaat gatattgaaa
      721 ctactccaat tatgcctcga ggagcatttc cacccgatgt tggtactagc ggacaccttc
      781 ttgtactagc aggaggagga agctataaat cacagccatt tagtggtgcg ggatggcgtt
      841 ttggaggtat gggtgtaaaa ccagctgatg gtacaggcaa ctttttacgt tggaaatcct
      901 acattgacga tggaagagta ggaaatgtaa atcaagccaa cgcaactaaa tctggttcta
      961 ttcaaggaat ggcattgtat gctcgtgaag gattcaaata ctttgcacca aacgggcctg
     1021 gttggacaat gaatataggt atttattaca cttataatcc tgttgcaggt acgtattatg
     1081 ttgttcaaaa tatttaataa aacagcacaa agagatttct ttgtgctgtt ttattatttt
     1141 tagttgttga caaacactaa aatataaaat ataatcaaca cataaacgaa cgtatgttcg
     1201 gcaagaaagc aggggatttt tatggatgag atggttttaa gtactcagaa atggctaaac
     1261 aagaaataca gtaatgttac agggtttgat aaggttccag aaaatgggcg aacagggtgg
     1321 cctacaatct atggacttat tgaaggacta caagtagaac ttggtattac taatctagta
     1381 gctaattttg gacctaccac tgaaaaaatg tacgataatc aggttactcc taaatggggc
     1441 aaaaatctac ctaaaaatat tgtatttctt attcaaggtg ccttttggtg taaggggatt
     1501 aatccaggtg gcttcgatgg ggtatatact ccatatttag atactgctgt aaaagagttg
     1561 cagactgatg cagggtttaa tggaagcgct gtaactggag ttttggatgc gaaatgggcc
     1621 aaagcgttat ttgatatgag tgcttttgtt cttgttcccg gcggagatag taaaattaga
     1681 aaaatgcagc aatggttaaa tgtaaattat ttagaataca cgggaataat gccttgtgat
     1741 ggtatttatc aaagaaattc aaatcaagca ttaatttttg cattacaagc tgaactagga
     1801 tattctccca gtgaagcaac aggggcttat ggtaatggga caactagtaa aacttatcct
     1861 gtgtctgaag gtaactcggg aaactatgta agaataattc aatatggatt atatgttaat
     1921 ggttattttg aagacggtac attcgatggt attttcacta aatatatggg tttagaagta
     1981 ctggcattcc gaaaatttat ggtattgcct gaatatactg aaatagcaga tgttgttgta
     2041 ataaaaggac tattgtctag cgctggagat attagaagat ctgcagatgg cgcagatact
     2101 tctacgcaat taactagatc tcaaatccaa acattggttg ataatgatat aaaaatagtt
     2161 ggtagatatc ttactggtac tgttggaatt ggaaaagatg aacgaaataa gtatctaact
     2221 acagaggaat tgaataatat tttttctaaa aatttatctg tgtttcctat ctatcaagat
     2281 ggaggtgcag cattagctta ttttacttat gatcgtggct tatcagatgg taagaaggct
     2341 atagatgcag caaaaaatct aaatatacca ttaacaacag ttatttattt tgctgttgat
     2401 ctagacatgt taggggaaga aattcttgca tatgctggag aatattttaa gggggtatct
     2461 gctgtaatgt catatagcgg gtatcaaacg ggtgtttacg gaacaagaaa tgtttgttct
     2521 caaataatca ataacgggta tgcttcgttt gcatttgttt cagatatgtc gactggttat
     2581 agtgggaatt taggttttcc tatgcctaga caatggtctt ttgatcaatt tgttgaattt
     2641 accataggaa gtggcaacgg agctgttggt atcgatttaa tcgctacatc gggtagagat
     2701 agcggattta atgagttatc aaatgatact aataatgata attacattgc caaatacaat
     2761 cagaaggtta taaatcagat ggtacgtgcg tatcaatatc tatcacaagc tggtcttgat
     2821 aatccttgga accctcattt gacattttat agatatgtaa attattctgg tttgtcatgg
     2881 gacatcattt cttctcctgt aacagagcat gatagaaaaa tttatgatga atataggaat
     2941 aaattaacta atagcgaggg cctgtataat tattttatag atcctaatac tggttcaata
     3001 ataggtttgc ctcacttaat tgtgacgtta caatcacaga tgtttctagc cgacacaatc
     3061 aatgatagtg tttctgactt tgcaggttgg ttaggagatt taatgacttg ctggggtgaa
     3121 gttaagaaat taggaataca aatggagcaa ggtgtatttg cattagtagg tacggctgca
     3181 ggtggaacat ttagtctaga agatttatac caagatgttg acgcggtaaa tgtttatacc
     3241 ttagcaaaaa gaaattctga ttcaacagct ttatcggctt taaaaacgta ctattgtgat
     3301 aagggtgcta ttgatagatt taatcaattt gttaagaaca gatttggttc ttcagataaa
     3361 atcgcttcag taacaaatag tttattagaa aatgacgaaa cagttgatat cttaggtgta
     3421 gggataattt catttttcat tgataaaata gttaataatt cacttgggac agattttact
     3481 tatcttgatt ttattggaga tgaggggaaa gttgcaaatg gattttctga aaaaattatt
     3541 caatttcaag aaaatagaag taaact
//
LOCUS       DAPCMT010000190         3481 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPCMT010000190 DAPCMT010000000
VERSION     DAPCMT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.194"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..2232)
                     /locus_tag="RD743_002694"
     CDS             complement(<1..2232)
                     /locus_tag="RD743_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564466.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="RD743_002695"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="RD743_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          979 a          770 c          548 g         1184 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 a
//
LOCUS       DAPCMT010000191         3455 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPCMT010000191 DAPCMT010000000
VERSION     DAPCMT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.195"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            322..579
                     /locus_tag="RD743_002696"
     CDS             322..579
                     /locus_tag="RD743_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDU0564467.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            811..1215
                     /locus_tag="RD743_002697"
     CDS             811..1215
                     /locus_tag="RD743_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDU0564468.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            1234..>3455
                     /locus_tag="RD743_002698"
     CDS             1234..>3455
                     /locus_tag="RD743_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDU0564469.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     "
BASE COUNT         1271 a          464 c          639 g         1081 t
ORIGIN      
        1 gtgcagatga taaaatcaaa aaaatacaat tgtacaaagt ccctatatta acaccaacag
       61 tacttacaat gttggagatg gaaaataata ttaattatat tgactcaggt tcagatttgc
      121 tgattaggtg gcaggtaata actaattttc ttatggaatt aaaaaaatac agtaatactc
      181 aaacgaagtt ctcttgaagt tataaagaat acaataaata taaatttcct ttagactata
      241 ttaaaatcag caatcgaagg aaaataaaag aagaaaatgg tacgcaaaat ctaactatta
      301 gagcaaaaac aggaagctgc tttggaagat agtcccgaat tgtttccgat gttaaaagat
      361 cgtttaaatg aattgactga aaaacgaaga cagttacaaa ttcgagagaa tgaaatctta
      421 gggatctttc aacaacaagg ggaaccgatt caagtcaaag atgtccaaca gatattgact
      481 agtttggatt gctttcttat ccagagtgag aagaagcaag ttaaagcttt gtatcacacc
      541 tttattgaga aaatcacctt tgatcctaat cagaagtaga tattgccgat cccgatgaga
      601 ttcacaccta ctgtgatttg ataattgaat gagtaatatc aaatagccgt atccaaacca
      661 aaggatatgg ctatttttgt gttagggata tacgtcacgt agcttgttat caagaataag
      721 tttctttatt tttttgcttt ttctaaaact gagtatgcta aaataagagg gtatatagca
      781 actttaaata agagatagga gtgtatcttt ttgggtgcag aattaaatca gaagttattt
      841 agtgcggcag ataatctccg tagtaaaatg gatgcgtcag aatataaaaa ttatttattg
      901 gggctgattt tctataaata tttatccgac cgtttgttgg aacaagtggt tcttttagca
      961 gatgaatcct tagaggaata tgacacagta agcaaacaaa cgatgttgta tcgtgaatta
     1021 ctttcagatg aagaatcaaa agaagatctg attgcaacaa tcgttgatat tttggggtat
     1081 gcgatttcgc cagagtatct tttcaacgtc ttagcggatc aagcaaaaca agcaacgttc
     1141 caattgaatg atctaaacaa agccttttta gcaagtcata tttatggaaa aatactgtcg
     1201 gcaaaattcg aatagtgtcg aggaggtgct gtgatggaag aattaaaaga acgcattgtc
     1261 aattatgtca tagagaactc tgagattaaa aataaatggg ggaacaatac agcacagaaa
     1321 atatctgaaa aattaaatgt tagtcgtaat attgtctctc aatatttgaa tgaattcgta
     1381 aaagatggag aatttggaaa aatcaatact cgtccagtaa tcttcttttc tttaccggag
     1441 aaagttccaa atcaacaaat ttttagctct tcagaggaag cggaaaaatg gtatgatagc
     1501 tcttttaggg ttggaataga aagggttatc ggaagtcaag gaagtctgcg aaatgtaatt
     1561 gctcaaatta aagcagcaat ctactatccg ccaaatggct tacccatttt acttcatggc
     1621 gagactggaa ctggaaaaag ctatttagca aattatgtgt ataaggattt gttggccaat
     1681 aattatatag aagagaaggc atctttcatt tcagttaatt gtagtgaata tgccaataat
     1741 ccagaacttt tcttgtccaa tttttttggg catgtaaaag gtgcatatac aggagccgaa
     1801 gatggaagaa agggtttagc agaattagct aataatggag tcttattttt agatgaaatt
     1861 catgcattga gcaatgaatg tcaggagaaa ctttttcagt ttatggatac gaggaaattt
     1921 catcggatgg gtgataatga aaactggtac acatcaagtt gctggattat atttgcaaca
     1981 tcagaaccac ctgaggataa actattatca actttgttga gaagaatacc tgtaaagcta
     2041 acacttccaa cattagacag tcgttctctt tatgaaagaa aacagttaat caagatattt
     2101 tttaaatcag aagaagaaag actagcaaag aaaatactga tatctcaaca attattagat
     2161 tttttattaa actatcgctt tagtgaaaat gttggcaatt tgaaaaatat cattcaattg
     2221 acctgtgcca atgcctttta tttcttagat gatttggaag aactttatat tcagatgtat
     2281 catttaccac aatgggtgaa tttcaacggc atgaaggtta tgaaaagaca gaatgaatta
     2341 aaatatattc aaatggatga tttggaaaaa gtagacgaag tttttaatat caacgaactt
     2401 atagagtatc tttatcaaac gtggaagaaa gacactgata agcagcagct ttttgagaaa
     2461 tatgatagat attttagaca aaagaatgtt gatatccaaa ataattcagc tttaacatat
     2521 cccacctatc aagagattcg atttagacag tatgttaagg aaatagagag agattttcaa
     2581 atagaatttg actattatta tcttcaaata acgtctatct ttttgaattc ttggatttat
     2641 caaatccaat taacatcgga cgataaatta tattccttca tcaatgatat taatagttgg
     2701 tgtagtcgag aagttcaatt agtcaatcgt tttattcaaa taggggatat ttatttaaca
     2761 aaaattcaaa aaaatgtttt ggttatttta tttaagttaa tgtgtagtaa agaatttcag
     2821 caaaaacgtt tagcactgat tttggctcat ggagatctaa cggcaacctg tatagcaagt
     2881 actgctaata ggatcgttga gagatatata tacgacgcca ttgatatgcc actaatgact
     2941 tcaagtagtg agattgcaga caaaattaat agatacttac aagagtgtac cggatatgaa
     3001 gaaattattt tacttgttga tatgggttca ctggagaaaa tttatctaaa tttggaacta
     3061 gacgctcaaa aaaaattcgg actactaaac aatgtttcaa caagaatggc tattgatgtt
     3121 gcaaatcaga tattattaga tttaccgctt aaacagattc ttgaacaagt aaaagaaaat
     3181 caaaatgtgg attatattta tgaatcatca aatattaaga aggatatgat tgtctgttct
     3241 tgtgcaagcg gattaggaac ttcgtttaaa ttaaaaaaga tacttgaaga tagctttcca
     3301 agggatactg atcttgtcat agaaacgtgt gactattact ctttagtgaa tgaaaactat
     3361 ttatcggagc ttcgtaaaga gaacaatatt ctttttattg ttggaacact aaatccacat
     3421 ctagaagatg tagaattctt ttcaattgaa gatat
//
LOCUS       DAPCMT010000192         3432 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPCMT010000192 DAPCMT010000000
VERSION     DAPCMT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.196"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            577..909
                     /locus_tag="RD743_002699"
                     /pseudo
     CDS             577..909
                     /locus_tag="RD743_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            923..1072
                     /locus_tag="RD743_002700"
     CDS             923..1072
                     /locus_tag="RD743_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564470.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="RD743_002701"
     CDS             1069..1515
                     /locus_tag="RD743_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564471.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="RD743_002702"
     CDS             1517..1654
                     /locus_tag="RD743_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDU0564472.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="RD743_002703"
     CDS             1691..1984
                     /locus_tag="RD743_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564473.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="RD743_002704"
     CDS             1981..2205
                     /locus_tag="RD743_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDU0564474.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="RD743_002705"
     CDS             2202..3221
                     /locus_tag="RD743_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDU0564475.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         1191 a          611 c          713 g          917 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc ag
//
LOCUS       DAPCMT010000193         3391 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPCMT010000193 DAPCMT010000000
VERSION     DAPCMT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.197"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(22..2493)
                     /locus_tag="RD743_002706"
     CDS             complement(22..2493)
                     /locus_tag="RD743_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDU0564476.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            2766..>3391
                     /locus_tag="RD743_002707"
     CDS             2766..>3391
                     /locus_tag="RD743_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564477.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          999 a          686 c          609 g         1097 t
ORIGIN      
        1 tcttttttga gatacttttc gtcaattttc tcgtttaaat atcgatagaa attttttagc
       61 tacgcgacta aacggctttt gctctttttc atttttacgt tcaaacaaat attctaccaa
      121 tgtggtcaaa tgcctaaaaa tcgaatctgc cgagaaaaat agaatgctca acagcaaaag
      181 tgatacagct gagataccgg tgattgaaaa cagcttatgc aaaaagatga ccgaaaaaaa
      241 gttaccgcta gcacagaaaa gaatgatctg acttctgaat ttattcagcg gtgaacagat
      301 tttatagaga acgaggaacc ctactgcaat caaaagcatg gtagatgctg tagaaacatc
      361 tgttttattt agattcaaag tcaccccgca gatcaccaac gcaccgacta cgatcatatc
      421 tgttagtcca cctggcacag ctttttccat cacattcatg atgaatttcc ctctgattcg
      481 ctttttgttt tcttctagag caagcaagaa agatggaagt ccgatagtaa acaaactaat
      541 caatgtgatt tgtgatggtt ctaaaggata ggtcagagca aagatcactg aaaacagtga
      601 gagcaaaaag gagaaaatat tcttcactaa aaacaagctg gctgatcgct caatattgtt
      661 cacaacgcgc cgtccttctg caacaacttc tggcatgcgt gaaaagtcag aatcaagtaa
      721 gaccacttgt gaggcctgca tggcggcttc atttccggaa gccatcgcta tactgcagtc
      781 tgcttctttc atggctagaa tatcattcac gccatcacct gtcatagcta ctgtattgtt
      841 acgctttatc aattgaacga agattttctt ttgttctggc ttgacgcgtc caaaaactgt
      901 atatttttcg acagctgctt catattcttc ttctgataat ttagatgcat caacgtactg
      961 atcagcatgt gaaatccctg cttggacagc tacattggat acggtgagcg gattatctcc
     1021 agaaatcact ttgatggcta cttgctgttt tttgaagtaa tcaaatgtgg ctcttgcttc
     1081 ttttctgata ggattagcaa tcagaatata gcctaatggt gtgactgctt ctgtcaatac
     1141 tggttcttca agaatcccat cgtacacacc aaagaccaac acgcgatagc ctttttcagc
     1201 gaaagaaaca aactcttccg catattccgg caattgatcg cgcaaaacca tttcgggcgc
     1261 tcctaaaaca tacaccttgt ctgccaacgc tacactgctg aatttttgga cagaagaaaa
     1321 aggaataaca tggtgatagg attgatcagt ggtttttgta aaatatgctt taacagcttc
     1381 cattgttgca ttgtctcctg ccatcgcttt tgcatagtca gcaatgcatg cttccaattg
     1441 atctgactga ctgattattt cttgtttttt agagacgatc attttttgaa cagacatttt
     1501 attttctgtg atcgttcctg ttttatctac acaaaggaca tttacccttg ccaatgtctc
     1561 aatgcttttc atattgtgta aaagaactct ttgctttgct agtttagtcg cacttaatgc
     1621 taatgcaatc gtcgtcagca agtacagtcc ttcaggtatc ataccgatca atgctgcttc
     1681 cattgcaacg acactttctt tgatcgtgtt tccattaaaa aagtaacttt gagaaaaaag
     1741 caggataccg ataggaataa tgatgatccc aatccatttg atcaattgat taagagaagc
     1801 aatcatctcc gactgttctc cttctcccat tgtcttggct ttgactgtca gctgggaaat
     1861 atacgaatct gctccaactt tgtctaatcg ggcgtaagct tttccggaaa caatgaaact
     1921 gccggacatt aatggatcac ctatttcttt ggttatttca tctgcttcgc ctgtcaataa
     1981 agcctcatta acttgcagtt tcccttccac gacctctgca tctgctggaa tttgatatcc
     2041 agtctctaaa ataacaatgt catctattac aaggtcttct atatctattg tttgttctat
     2101 cccgtctcgt atgacattga cttttgctgt atttaaaaca tttagttcgt ccaagatctt
     2161 tttggatcga atttcctgaa aaatggcgat tcctgtattg gctatgataa ctggtaaaaa
     2221 agtcaagttc ttataggatt ctactaaaca aagcaaaatg gctaagataa gaaagatcaa
     2281 attgaaatac gtaaatatgt tttccaatac gatttgctga tttgttttga atgtgggatc
     2341 gactgaatta tttgctgcac ctttttctat tcgttccttg acttgatctg ccgttaatcc
     2401 ttctttatac gatggaaaaa gacggatgac tttatctgat gatctttgtg ttactttctt
     2461 ttttttactt cgatttatcc atttattctt catgtggcta cctcgattga tttcgttttc
     2521 tggatatttc caaacttctc ctatccagta tagaacagat atggaaagtg ttcctcacat
     2581 taagcttgca aacacctttc attcctgtca tatagcaaaa aaaaagggtt ctataatatt
     2641 ttgtgttggt ggaaaatcga aggcgatttt ctactaacac tttttcttcg tttcaactca
     2701 aaaagagact cacttttggt aaaatttagt cgaccaaaac gtcatcctac caaaggagtc
     2761 tctcaatgga taatcatact agaaaattac tcggattaac agataaacat ttattttttg
     2821 atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt attaaaggaa
     2881 aattggatga ctcttcttcc tattgtgaac actgtcgatc ggttcgtatt atccgcaacg
     2941 gttcctatac gacacgcact cagatgttga aagtcaaaga aaagttaact attttagaat
     3001 taaaacgtac ccgttttctc tgccatgatt gtggaaggac cttttctgcg aaaacggact
     3061 tagttgatga acatcatcaa ctaacaaagg aactgaagca ggtgattctc atggaactgt
     3121 acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat gggacggtga
     3181 cacggatctt gcgtgaagca acgaagcatt accagccacg tatgaacttc cttccgacgg
     3241 tgttgtgtat ggatgagttc aaatcaatga attctgtttc tggttccatg agttttattt
     3301 gtgttgatgg gacaaccaat cagctattta cgatccttga agatcgcaga ctttacaaac
     3361 taactcaata cttcctgcgc tttcctagaa a
//
LOCUS       DAPCMT010000194         3329 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPCMT010000194 DAPCMT010000000
VERSION     DAPCMT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.199"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..189
                     /locus_tag="RD743_002708"
     CDS             <1..189
                     /locus_tag="RD743_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDU0564478.1"
                     /translation="LVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIGKVLGITRQ
                     GATNLKHRLYKKIQKMLV"
     gene            283..474
                     /locus_tag="RD743_002709"
     CDS             283..474
                     /locus_tag="RD743_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564479.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            988..1320
                     /locus_tag="RD743_002710"
     CDS             988..1320
                     /locus_tag="RD743_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564480.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1616..1771
                     /locus_tag="RD743_002711"
     CDS             1616..1771
                     /locus_tag="RD743_002711"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDU0564481.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2047..3329
                     /locus_tag="RD743_002712"
                     /pseudo
     CDS             2047..3329
                     /locus_tag="RD743_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         1146 a          614 c          601 g          968 t
ORIGIN      
        1 cttgttgaag atataaaatt agcaaaaatc cttcctttgt tgaaagaacc tgaacaaatt
       61 tttcttatcg aaaaatttat ctttgataaa actgataaag aaattggaaa ggttctgggg
      121 attacaagac agggtgcgac aaatttaaaa catcgacttt acaaaaaaat tcaaaaaatg
      181 ctggtttaat atctgcataa taactaattt tgtatgcttc tttgctcgtt ctcctccttc
      241 ccaaagaaat agattataag tccatgggaa gaaggtagat ttatgaattc tttaaaagaa
      301 gatttcattt tagcaaaggc tggaaatgaa gaagctgttg aggcaattct caaaagattt
      361 agctctttga ttcataagca atcctggaga actggaaaat atgatcaaga ttgttatcaa
      421 gaatgtatgc ttgcaatata tctagctatc tcgaaattcg aaattaaaga ataactcaac
      481 taagggagtc tgtgacataa gtaaaaagat agtttcaaat ttcgaacaat aaatttgaaa
      541 ctatcttctt tataattata aagtatcgat tttacagcgt tctataatga ttcatagaat
      601 ctaaaacgaa tgattttaat tatcaacgtt ttttaattcg tttttagctt aaaaataact
      661 tttgtcacag tctcactcct aaaattactt tatttacgta tgaagtgggc aggaggtcga
      721 atccagaact gggctatggt acttcaacga ggacttctcc tctagaattg aaaagcatgc
      781 gatttacctt ccgtaacgac ggtagattta ttcaactatg tgtccaatca aaatttgtct
      841 gatttcaaaa atagtcaaga gaattttggt tgttaaaaac tcttatctat caacttccac
      901 agtttagttg acaaaccctg atactaagtt gggctatgat acacttttct tgactatcag
      961 ttacgttacc ggaaggaatg aactctattg agaaaaacat atgatcgtaa atttaaacta
     1021 aagatttcac aggatatcct tgagaaaaaa gtaactacca agaaaattgc tgaagaatac
     1081 aatatctctc gtccaacaat ttcccgatgg gtttcagaat atcgtcgata cgggaaaaat
     1141 gcttttgctg gacaaggaaa aagattacct gataaagcag atttctacat tcttgaacag
     1201 gaaaataaac gactaaaaga ggaagacgag tgttctacga acacaaagat cgatacggat
     1261 ctccagggat cactcaaaaa ctttacgatg aaggaatcga aatcaataaa cgtgttgtag
     1321 ctgttcttat gcgtgaaatg aatttatgca caagaggatt tcatcgacgc aaatcttctc
     1381 atggtagaga aaaaacaatc aaagaaatgg taaaaaaatg ttgaatcgat aatttgaaca
     1441 gaaccaaatt gatgctgttt gggtgatgga tattacttac attccatgta atgacggtcg
     1501 cctctatttg tccacctata ttgacttagc aactcgtatt ctacgttact atatggtaga
     1561 ttcacacatg aaaaaagaga ttgtcgtccg tccattacaa atttattaag gagaattgcc
     1621 tacggtaatt cattccgata gaggtagcca gcatcggtct tttatttatc aagagctgtt
     1681 gaacgaccac cacatcacac attcagtgag ccaaactggg actcctgttg acaatgcagt
     1741 aattgaagcg tttcatcgat caatcaaatg agaattaatc gactccaaca aatataaaag
     1801 taaaatagag atgaaggtgt taatccaaga ttatttagaa gactactacc caaacaaaag
     1861 aatccataca aaatttatga tgactccttg acaatttgaa gcgaagaaaa taagggatct
     1921 ataatatcta gtgttggtga cgccttctgt tgccaatact ttttcttttt tctaataaac
     1981 gcaaacagac accttaaaat cctttagaat aaagtcgacg aaaaccattc aaaggagtgt
     2041 ttcaagatgt cctattcaga gtttaccaaa gagttactag atattctaga cctaaatctt
     2101 atctttcatg aagatgcctt tcgcaaagag cgaatcaatg acgaaacatg ttttgttttt
     2161 gatggcacat tgacctatca accggaggaa tgctttcatc gccactatca aaatgaacaa
     2221 acgatcatta aatagggctg gaaaaaagta tccatcttat tgaatgatgt cagtaactac
     2281 aagaccattc tgcggattaa caaacagcgc ttcaaatgta aacactgtgg aaagactttt
     2341 ttagcagaag attcagttag tgatcgtcgc tgctccattg ctcgcagagt caaacaagcc
     2401 attcttgagc tattaagcga acctctttct atgtctttga tcgcacgtat gaagcacatt
     2461 tctccgacca ctgtcattcg aatacttcgc agccttcggc ctaaaaccat ttctttgagc
     2521 cagctgttac cagaagtggt gtgtttcgat gaattcaaat cagtgaaaaa tgtctcggga
     2581 gccatgagct ttgtcatgat ggacggcaag acccatcaac tgattgatat tgtggaaaat
     2641 cggcaactca atcctttacg cgactatttc ttgcgctacc ctataaaagt ccgtgaacaa
     2701 gtgcgtctag tggtttcaga tttctataca ccctatcgca cattggtgaa agagtgtttt
     2761 ccgaatgccc gaatcgtcgc cgatcgcttt catattagcc aacacattgg tcgggctttt
     2821 accaaccacc gaattcaagt aatgaagacc tttaaaaaag gggatcgtcg gtcgaagcac
     2881 ttaaagaaat actggaaact acttcaaaag aatgcgtggg aattgaaagg tcagcatcgc
     2941 tactggcgtc cgtcatttcg agatcatctg acagaagcag agatagtcga tcggctatta
     3001 tcttatgatg attccttaaa gcgaggctac gaagtctacc aggattttct ttccgttatc
     3061 agaagacaag atgttcctga attcgtcgcg ctcctaaaag aagactacaa ggagttgcca
     3121 gagcactacc aaccagtgtt cacgaccttc aagaaatacc aaacagaaat caaacgagcg
     3181 ttgcgcgttc cctattccaa cggccctata gaatgtttga ataaccatat caaagtacta
     3241 aaacgaattg cctatggcta tagaaacttt caaaactacc gagaacgaat ctttttatat
     3301 cgaggaaaat attttaagaa aactaagaa
//
LOCUS       DAPCMT010000195         3221 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPCMT010000195 DAPCMT010000000
VERSION     DAPCMT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.200"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            38..250
                     /locus_tag="RD743_002713"
     CDS             38..250
                     /locus_tag="RD743_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564482.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(528..2327)
                     /locus_tag="RD743_002714"
     CDS             complement(528..2327)
                     /locus_tag="RD743_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDU0564483.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2451..3047)
                     /locus_tag="RD743_002715"
     CDS             complement(2451..3047)
                     /locus_tag="RD743_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDU0564484.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1152 a          590 c          573 g          906 t
ORIGIN      
        1 aaaaaacaaa ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag
       61 atcttatata tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac
      121 atatatcaac acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat
      181 caatgaaagc aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctgctgaatt
      241 ggtttcttaa acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac
      301 agaagggagc gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt
      361 gcagtgatct ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga
      421 gaaagttaag cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt
      481 tatgcgtttt ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc
      541 aaaacaaaat ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt
      601 tcatttagta gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga
      661 ctcatagcat ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca
      721 gaattaatga agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc
      781 acagggggcc caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct
      841 tcttttaaat ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag
      901 ataactttgc ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag
      961 gtcttgatct caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca
     1021 aaagaacaaa caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc
     1081 aatgtaagat cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc
     1141 atcactacga aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga
     1201 tagtctctgc ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc
     1261 acacctctgt gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata
     1321 ttgtaaagac agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc
     1381 agcgcagaga aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag
     1441 aagccttcgt catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg
     1501 atattgaata ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca
     1561 tagtctggga aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa
     1621 gaagaaagga gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga
     1681 cgcgatttgg tcaatgacca gctttcccga atggctgctc gaaaaggacg atctcgtaga
     1741 atcatttccg gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg
     1801 agtaaaaagg aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg
     1861 gggattttta tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc
     1921 aataaaaaat aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt
     1981 atcgtcaagt gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca
     2041 ctcatcaaaa gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg
     2101 attaacgaga gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac
     2161 aaatagtcaa ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa
     2221 caaataaata aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct
     2281 tttaaaaagt cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct
     2341 cttcacattg tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc
     2401 tttcccagat ttaacattgt caatcaatga ctaattttac aaaataaata ttatccaaga
     2461 agaccactta gatattgacc tccataaatc aaggccatac tatataagaa aatcgacagt
     2521 ggtttagcaa tcaaaatgat tagtgtatat cgtttcactg acatgtttgt caatccagcc
     2581 atcaaacata acgcatcgtc aggagcaact ggaagaaaaa tagctagagc aaacagcttc
     2641 tcaaaacggt tttgattatc cagccagcca atatatttgt tatatgtctt atcgctaatc
     2701 atggaaagta tgaatggctt tccataacga cgccctagca gaaaaatgat aatcgaacca
     2761 atgcagattc caatatagtt atagataaat ccagcataag ggccaaatat caggacacca
     2821 gctgcagtac tgatccctcc aggaatgatt ggaataacta cttgcaggat ttggatgaag
     2881 acaaaaataa tcggtcctaa aatgatggaa tcacctacta atcctcttaa cgcattcaaa
     2941 tctttgaaaa cacctaaatt aataaaataa atcgtcagcg cgattgatag gcctaatccg
     3001 ataatggata tgaaatttat cagttttcga gaggcagcaa tattcattat tcatcaactc
     3061 cttagaggtt ctttgttaac tatactttac tagaaaagca actaactgcg ccattttttg
     3121 atatgacaga aaacaaacaa ttttgtaagt tcatcataag tataaaagat tgatctcaaa
     3181 aaaacatcca actaaagact tatttaataa cttgtttttt t
//
LOCUS       DAPCMT010000196         3128 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPCMT010000196 DAPCMT010000000
VERSION     DAPCMT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.202"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            103..1347
                     /locus_tag="RD743_002716"
                     /pseudo
     CDS             103..1347
                     /locus_tag="RD743_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="RD743_002717"
     CDS             1360..1992
                     /locus_tag="RD743_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDU0564485.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="RD743_002718"
     CDS             2197..2424
                     /locus_tag="RD743_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDU0564486.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="RD743_002719"
     CDS             2493..2759
                     /locus_tag="RD743_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDU0564487.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="RD743_002720"
     CDS             2749..3018
                     /locus_tag="RD743_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDU0564488.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DAPCMT010000197         3096 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPCMT010000197 DAPCMT010000000
VERSION     DAPCMT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.203"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..131)
                     /locus_tag="RD743_002721"
     CDS             complement(<1..131)
                     /locus_tag="RD743_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDU0564489.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVC"
     gene            1008..1262
                     /locus_tag="RD743_002722"
                     /pseudo
     CDS             1008..1262
                     /locus_tag="RD743_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1332..1844
                     /locus_tag="RD743_002723"
     CDS             1332..1844
                     /locus_tag="RD743_002723"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564490.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1841..2878
                     /locus_tag="RD743_002724"
     CDS             1841..2878
                     /locus_tag="RD743_002724"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDU0564491.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1106 a          554 c          601 g          835 t
ORIGIN      
        1 tgacaaacat ttattccacg atcatataac aattcctgaa cttcacgata gcttagattg
       61 taacgcaggt agtaaccaac agcgacaata atgacgtctt ttttgaattg tttgccttta
      121 aaatgattca ttactctgtc ctctctgtct tttttctcaa ttttacacta aaatagattt
      181 tttggaaaac tttgcaacag aaccgtttta ttgatgacca cgaaagtgca tggcgatatt
      241 tgatgttgat actttatgca gtaccctatg tattcttcgc tatcattctg tggctcatag
      301 cattgttttt tagagtattt aatgaccttt aggtagattt atatataaaa agggctaaat
      361 acgcaaaata cccccttaaa atgcaaaata aatgcaaata agggggtaac tccttatgaa
      421 aaaacaacta atagtattat aacacgtttt tatgccgaga aaacttattg gttggaatgg
      481 gctatgtgtt agctaacttg ttagcgagtt ggttggactt gaattgggat taatcccaag
      541 aaagtaccaa ctcaacaaca cataaagccc tgtaggttcc gaccaataag gaaattggaa
      601 taaagcaacg aaaggagttg agtaaaatga gtgagaaaac gaagaaaatc attcgagtat
      661 cattagcttt ttcacaagta gtagtgcaat cattgatagt ttattatact taccagcaat
      721 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
      781 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      841 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      901 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      961 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
     1021 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
     1081 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
     1141 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
     1201 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
     1261 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
     1321 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
     1381 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
     1441 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
     1501 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
     1561 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
     1621 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
     1681 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1741 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1801 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1861 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1921 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1981 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     2041 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     2101 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     2161 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     2221 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     2281 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     2341 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     2401 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     2461 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     2521 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     2581 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     2641 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     2701 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2761 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2821 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2881 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2941 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     3001 ggaaaataaa aatacgacaa ctatattgac aaacaccgac catcgtccag taaagagacg
     3061 caataaattc tatcgaagtt tacgcactgc ctctac
//
LOCUS       DAPCMT010000198         3081 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPCMT010000198 DAPCMT010000000
VERSION     DAPCMT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.204"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(301..915)
                     /locus_tag="RD743_002725"
     CDS             complement(301..915)
                     /locus_tag="RD743_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0564492.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1521..1898)
                     /locus_tag="RD743_002726"
     CDS             complement(1521..1898)
                     /locus_tag="RD743_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564493.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2198..3079)
                     /gene="galU"
                     /locus_tag="RD743_002727"
     CDS             complement(2198..3079)
                     /gene="galU"
                     /locus_tag="RD743_002727"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDU0564494.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          933 a          643 c          476 g         1029 t
ORIGIN      
        1 gtgtattaag agacaagaaa gaatccatat ggattctttc ttgtctcttt ttccactact
       61 ttttattttt ctgacactac atattcttat aactcatttc tcataactgt gaaacaactt
      121 ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc atcttaaaat
      181 aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt ctttttttca
      241 aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac atgtggataa
      301 ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa cttgctgcag
      361 caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat ttggcaaagt
      421 caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa
      481 tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc cagtaacaat
      541 gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg ccgggtttct
      601 tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct gatcaataat
      661 tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt caatcaatat
      721 acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac ttagtgtgcg
      781 gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc aagtgccagc
      841 ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat aatagtcaaa
      901 ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata gaaacagctg
      961 cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg tggtctttgc
     1021 tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca tagcaaataa
     1081 aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc ctagattagg
     1141 tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt cagggtgtat
     1201 gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga gccttttttt
     1261 attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg caagcaagaa
     1321 aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa aaggaatggc
     1381 ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt gaaaaaagct
     1441 tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt ttatcttttt
     1501 ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg ttcgagagaa
     1561 tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga ctttcttttc
     1621 atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa tacgcaagct
     1681 tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac
     1741 cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat aaatagacta
     1801 ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca cttgagtata
     1861 tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca ccttttttta
     1921 taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct ttcatttctc
     1981 ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag cataatattt
     2041 tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc aactgatcta
     2101 gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc tttaataaca
     2161 aatagccttt cattctcagc aggccactta tttccagtta actttctgtt cttagctttt
     2221 tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt tgaagtccat
     2281 attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt tttcctttaa
     2341 actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc gttagctgta
     2401 tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta ggagtcagta
     2461 gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt ttttctacaa
     2521 acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata attccgtatt
     2581 tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt gtttcttcat
     2641 aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca tcccccagca
     2701 tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca tcccccagtc
     2761 ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct tcaactaaag
     2821 cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata tttgcatcaa
     2881 agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct tcgattcccg
     2941 aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt ggaagcattt
     3001 cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg gcaggaatga
     3061 ctgctttttt gaccttcact t
//
LOCUS       DAPCMT010000199         3076 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPCMT010000199 DAPCMT010000000
VERSION     DAPCMT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.205"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            62..433
                     /locus_tag="RD743_002728"
     CDS             62..433
                     /locus_tag="RD743_002728"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDU0564495.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            613..1035
                     /locus_tag="RD743_002729"
     CDS             613..1035
                     /locus_tag="RD743_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564496.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            1180..1425
                     /locus_tag="RD743_002730"
                     /pseudo
     CDS             1180..1425
                     /locus_tag="RD743_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1534..2490
                     /locus_tag="RD743_002731"
     CDS             1534..2490
                     /locus_tag="RD743_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564497.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
BASE COUNT          962 a          515 c          682 g          917 t
ORIGIN      
        1 cttttttttg ctgtattcat ttagattatg atagagaaaa tacgtgagag ggggacctgg
       61 gatgtggcta tctgatgaaa aaattcagtg gcgtagctat cagaagcgta tattgactaa
      121 ctttgctact tatttaaata acaattcagt ccatctagtg gctccaccag gggctggcaa
      181 gaccttttta ggaattgaga tgatccgacg tttggattgt aaaaccttaa ttttggttcc
      241 ttccctaata ttgaagaaac agtggttaac aacaatcgag gaatatttct tagtaaatcc
      301 agcagatagt tgctatcttt ctggggcgat tgaacatcct agtcatatta cagtggaaac
      361 gtatcagacc atttacaacc gactgaagga ggagccaact tatggtgatc agctggagct
      421 gttagttttt tgatgaagct catcatttaa aaaaaagctg gggtgaattg ttgattgact
      481 tgaagaaaaa acaaccgaat ctgttgacga tttccttgac agccacgcct ccgattgata
      541 gctctcagac ggagtggaag caatatattg agttaaaatg gcctgattga cgaagaaatt
      601 agcattcccg agttggtaaa ggaggaggtt ctatgtcctc atcaggattt tctatatttg
      661 attccggcta atcaggaaac tactcagcat tattttacct ttttagagaa acaaaatgag
      721 ctggtggaag cgttaatgga aagccaagag acggtggatt atttgctttc tttagaattt
      781 gttcagcatc cgcttgagca ttcgaaggaa atttatgaaa attttgagct gttcactagt
      841 gcattaattt acttgaatca tcataactat gaattgatac ccgaacattg gcagctatta
      901 ggcagttcta agcagcaagg taaaaaagct tcagttccat ctttaggtaa aaaagagctg
      961 atcactattt atcagcagat gtttgaagat caaccggaat tgcctatttt ttatcaattg
     1021 aaaaaagctg gatgattgtt tgatgagtcg ctgcatttgt ttccagagta tccagacact
     1081 gttggtagtc aggcgattcc tgagaaaaag caagcagttt gtcaaatttt gttaaaagaa
     1141 gaagttcagc tgaagcaaga actgacagcg gtcattttga tggatcgtat ttgcaaggag
     1201 gtattagcag gcagtgatgg taccttagct tatggcgtgg ttcctttgtt tgatgaagtt
     1261 gttcgtctat taggtaacac tcagcaaatc ggtgccatct gtggagagtt cttactgtta
     1321 ccgaaaatgc tagtaactgt tgagctggca gaactagcga cgggtatgaa tgaacatcct
     1381 tatatgtcgg gttattttta tttgacggtg gatgacaaaa accgccaagc attgatgcaa
     1441 gcagttactc gcctattaac cgaaaagaag ttgaatattt tggtaggaac cgttagtttg
     1501 ctaggagaag gctgggattg tcctgcaatt aatctgttga tcttggctaa tcaagcttcc
     1561 acttttgtgc aaacacaaca actacgaggg cggggcttac gcctaggtgc acaaaataaa
     1621 gtaacgaaca tttggcattt agcgattgga ttaccgggag ttcctctgtc tgaacagcct
     1681 gatttgaaaa gaatgctacg gcgtttgggc aatatttcag gtctgacctt tgaatcttca
     1741 ccaaaaattg agagtggtgg cgatcgcttt gctttaccac tggaatatac gaccaccacg
     1801 atggaaactt atactcagtc gatgttctat ttttcttgtg aacgaagtcg gttgaaagaa
     1861 caatggagta ctgctttagc tgatggctgg cacctatcta tgccaatcgt cgttaggaaa
     1921 gtcgatcagg taaagcaaaa acaagaaact tcattgccga tagtagtaaa aaatatagag
     1981 cctctaagct tctggtcagc tatatttaaa ggtaacttgc tggaatattt ccaaattcgt
     2041 aggcgacaaa agctatggga aaaggaatgt gcccgcaaaa aaagcttggc catatgcgtt
     2101 caaacgacgt tgcagcgcct tggtgaagtt agtgatagaa aagtaatcgt agcggctgat
     2161 cagcaggaat ttagatgcta tcttgagaat gctggctatc gagaaggaca gctttttact
     2221 gaagctatga gggagctttt agcaccgctg gagaatccgc ggtatttaat cgaagggaaa
     2281 aagatagttc tgacggtacc agaacgcttt ggccgaaata agcaaatcgc taaccttttt
     2341 ctaaacgaat taaatcaatc gtcgctgccc aaatgtcagt tggtgtatac tagaaatcct
     2401 gaaggacgcc aaaagctagt gacttatcga atgaggcaag tcttccagac ccgcgatgaa
     2461 gtaattattc aaaaaaacat ttggacgtaa gttgatgaaa ataaattttg ctagagtaga
     2521 acaagtccga gaattaagtt gctacttaac tgataatgcc aaggacagga gaatcttttc
     2581 ttgacgactg aaagaaacta tttgacttat ataacggtgt gtgatgaata tttgcaaggc
     2641 attctcttaa aaagtactcg taatagtttt tttgaaaaca aaacagtact ttattaatag
     2701 gactgacaga gcgatataag aacatctgca gacagaggtg aatgcttttc tcatgatcga
     2761 tgaaacaatg gtacttcgga tattcttgct tttagtttga accgttgtga ttttctttga
     2821 ttcctaaaag tagttttata gaatgaaaat caagcatacg gaatgctcct ttgattgtgt
     2881 tgtggtgatt caatctaatg gagcttctat atgttttttt atttgcaaaa agaaccgata
     2941 gaatattttt atcagtctta gaaattatag agccaagaaa tttcaagcac acataaaaac
     3001 gcgtgtttta tgcattttat gagcaaaaat aactaaagac cattacgtaa aacataggta
     3061 aatcagcatt caatct
//
LOCUS       DAPCMT010000200         2940 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPCMT010000200 DAPCMT010000000
VERSION     DAPCMT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.206"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(79..621)
                     /locus_tag="RD743_002732"
     CDS             complement(79..621)
                     /locus_tag="RD743_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDU0564498.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            756..1145
                     /locus_tag="RD743_002733"
     CDS             756..1145
                     /locus_tag="RD743_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564499.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1149..1913
                     /locus_tag="RD743_002734"
     CDS             1149..1913
                     /locus_tag="RD743_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDU0564500.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2042..>2940
                     /locus_tag="RD743_002735"
     CDS             2042..>2940
                     /locus_tag="RD743_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0564501.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQG"
BASE COUNT          947 a          493 c          605 g          895 t
ORIGIN      
        1 tttaatcaaa ataataaata aaaactgtct aacatttaaa atttaattta gatattagac
       61 agtttttaag tttgtgagtt attttccttg tcgttttctc acttttttag tccagaaacc
      121 acctgaaata tattttggct cataagaaat gataaaagca cgctcttcga tttctttgat
      181 caggcgatac agttcacgtt cgctttttct aggagaaagt atttcgagaa ctagccgctc
      241 accttccagc cccattgcag aagtctgtgt cacaccataa ccgtgttctc tcagtatacc
      301 cggcaaactt ttatcttctg cgctatttgc tggcaaaatc gttgtgacca tgatgtatcc
      361 taatgccaat ttgtcttcaa tcaaaattcc gacactgata cctactgcgt aacctaacgc
      421 atagacaaat aaattcagtg gattatctaa acgatctaaa acaagggata agccgacgat
      481 ataaatcgtg atctcgacca tactgacaag tggagcaatc agtcgatagc ctttcattgt
      541 caacatgaaa cgtaacgtgt tcagagtgat ataagcaaaa ttaatgagga agatcatccc
      601 caacattttt ggatcaatca tttgtaaaac tcctttcatt tatataacaa caatatcaaa
      661 aaaaatcgta agtgaagaaa aattgaaact ttatacatac aaaaaaatga taaaatgatt
      721 ataatagaat tataaagata ggttaggtga ttaaaatgaa aacggtcaat cgtgtgtttt
      781 gtgccagcca cgtgctaagt ctccttctca gtatatactt aggtgtctat tcgattcaac
      841 agccaattga ggtagagaac attgtttttc agatgtcgct gacagatggt ttaattttaa
      901 gcgttttttt ctttttgata ctgtttattg gtaatatttt cggtagcgtt gccagttttt
      961 tgaatttttc tatttatcca ctcctttcgc tagcattagg agtggtggga ctttgttctc
     1021 tgtgcttatt tcccgagcca ttcagtcctg catttatatt atttggtaca ctgaacctgt
     1081 tccagacaac tgttggagca tggttgctat ggcgatcagg aaatttaatg aaaataggtg
     1141 agtaacaatt gaatccaatt atattgaaac gttctgtcca tcatattcca gtgttagaag
     1201 ttgttctgaa agaattaaga gaggaacgta ttcccgttgt catctattat cacggatggc
     1261 agacaagtaa ggagttagtt ttgacgcaag gaagaaaaat tgcgcagcag ggcattcgtg
     1321 tgatcctgcc agatgcgatg aatcatgggg agcgcaaaca gccggtttct tctattccat
     1381 cctatacatt ttgggatagt atctacggaa atctgtttga atttgacaca cttatcaagc
     1441 atttagaaaa aagaggtctt ctacatgatc agcttgcagt tggcggggtt tcgatggggg
     1501 gaatgacaac atgcgccttg ttggctaaac atcctcatat agtagcgggc gtgtgtttga
     1561 tgggttcccc tgcacctttg gaatatggaa aagaaatcgt aaggcgcgca tcggaatata
     1621 attatgtcgt accaaaagac tattttgatt tgatttcatg ggttaagagc tatgatttgt
     1681 cattgcagcc ggaaaagtta gctggacgtc ctctgttctt ttggcatgga caagctgacg
     1741 aaaaaatccc ttatatacaa acggaacgat ttgtagaaaa aaatcggcat aagtcttacg
     1801 gaaaagaaat tgtttttcgt tcctcaaaaa cacagcgaca tatggtacaa ataccactaa
     1861 tggaagaagc ttccactttt ttagcaaaaa atttgataaa aaagtataaa taattgaaac
     1921 ggaaaatcac tcatgacgat agaggatttt ccgtttttta ttttgggcta taataaactc
     1981 tgttaggaaa atttggtata attgatagta agaatgacaa catgttagaa aggaagagag
     2041 catgcccact ttagcagatg ttgcaaaacg tgccaatgtt tcaaaaatga cggtctctcg
     2101 agtcatcaat catccggaac aggtgactga cgaattgaaa gagttagttt tctcagcaat
     2161 ggctgaactg gattaccgtc caaatattgc tgcaaaagca cttgtcacga atcggtcgca
     2221 gatcatcaaa ttatttatac tggaagaaat cgacacaact gagccctatt acatgaattt
     2281 gctgatggga atcgctaaac ggatcggtaa agcgcattat tctctccaat tagttacaaa
     2341 tgatgccttc gacgtcggtt cttgtgatgg atacatcatc acaggtgtca gaaaaaaaga
     2401 cttcgattgg atcgaccgtt tggaaaaacc agttgtcttg tttggggaaa attctgaagg
     2461 atatgattat gtagacagcg ataatgaata tggtacagca aaagcaacgc aatatgcctt
     2521 agaaagagat tatcaaacga ttatttatat cggtatcgaa gtggacgagc cttttgaaat
     2581 ttcaagggaa actggttatt tatctgtgat gaaggaagtt gatcttccta gtaaaatctt
     2641 gcggtttcca aatcattcga cagaagtaga aagatatatg acagaaaact gggcagattt
     2701 tacagataaa acttgttttg tatgtagttc agatcggcta gcactaggag cagtaagagg
     2761 gattataaat gctggaggac acttgcccga agaatttggt gtgattggat ttgatggggt
     2821 atttttagac caaatcagtt cacctaaatt gacaacgatc aagcaagata ttatacgtat
     2881 gggtgaagta tgtggagaaa tgttgttaaa aaaaatagaa gaaaaagggg cgagtcaagg
//
LOCUS       DAPCMT010000201         2929 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPCMT010000201 DAPCMT010000000
VERSION     DAPCMT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.207"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..719)
                     /locus_tag="RD743_002736"
     CDS             complement(<1..719)
                     /locus_tag="RD743_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDU0564502.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILF"
     gene            complement(730..2106)
                     /locus_tag="RD743_002737"
     CDS             complement(730..2106)
                     /locus_tag="RD743_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0564503.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(2235..2882)
                     /locus_tag="RD743_002738"
     CDS             complement(2235..2882)
                     /locus_tag="RD743_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDU0564504.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
BASE COUNT          971 a          591 c          352 g         1015 t
ORIGIN      
        1 ttaaataaaa tatcagttct taagttttcc catgattctt tttctttatt aaccaaaata
       61 ggataaaaga aaactttatt cttatttgca gcttctagat caccaggaga atcaccaatc
      121 attaatatct tatccaaatc acaaccattt tcaattacac ttcgaataat gtcttctttt
      181 tttcctttat cttgacaaca cagttcatca actaaggata ataattcatg tctttcccat
      241 tcttcttgaa tagcttcctt attagctgag cttacaataa ttatcttccc atattttttc
      301 agagctttta tagtatctct agcacttaaa aagggtttat ctaatccttt agcttgacta
      361 atctgtcgat tgacttcttc cgaccatttt aaagctttta ataaatcctc tgactgagag
      421 tattctattt ccctcttcaa agatggatta gacaattctt ttgttacaga tacccagtgc
      481 tttaatcgat taatcgatgg tacatcattt ttttctaatg ttttaactaa acctaaaaat
      541 ctgtttattc cacgagtctc tgaatataaa ttaatattat tccaatcctg taggaatttt
      601 tccctatgat gaacatcaaa aatctctgca gcaattggcc cgaaaaagag attgtgttta
      661 taagtcattg tatccatgac acaaccatct gaatcaatac aaaatatcca actgttcatg
      721 ttttttctcc tagttatgtt tttgatttag caaattataa ttttctaata ctttttcttt
      781 agataatgga tatacgaaaa ataaaatcaa tcctacaaca aagaaacaaa ctgctggaat
      841 caacgttgaa gagttataca gcttttgtaa aacttctcca ctttgtactt ttgctaacga
      901 atcataccct attatagaaa gagtccaacc tccgacaccg ccagctaaag cttgtccaat
      961 ttttcgggca aaagaatata ctgcataaat agttccatct tctctttggc cagatttcac
     1021 ttcttgatca tcgattacat cagtaatatt cgcccaaatg actgtattaa aaatacctaa
     1081 ccccatatat ccaacagaag taagaactaa gaatacatac atattactag gctttatcaa
     1141 aaataaaagt aaatatacaa tagcagagaa gaacatccct actgacgcca attctttttt
     1201 accgtatttt ttactgaaaa ttaaacttac aggtgaaaca agtaccaaca tcaaaatagg
     1261 attgataaag ttcattaaaa ctattccctg tgcattatta taataatctg gaaatacata
     1321 attgttcata ctcattgtta aaagctgagc aagcaacaaa aaaatagctg cagctattat
     1381 agaaagcaac gctctatttt tagctaatcc ttttaaagat ttcaacaatg aaacttttaa
     1441 ctgcgattca tcacttttaa cccgttctac agttaattta taacatatta cgtaacaaat
     1501 aactgcacac actgaaaata ctcctgccac aattgtaaaa gttgatccac cacgtacaat
     1561 ttgagctcca gaactatctc ttgtataaat aaaaattggt gttaacacgc caatgaacat
     1621 acctgctaag gtagctccta tactacgaaa tgttgataac gctgtccttt cttctgcaac
     1681 agaagtgata gctgatgcca ttgaaccgta aggaatatta atagatgtat aaaaaatact
     1741 tccccatagt atatatgtta catacatata tataacttta atactcatag aagctgatgc
     1801 cattgatgtt tgatacatta aaaaacttgc taaagcgact ggaccactca tccgtaaaat
     1861 ccacggttta aatttgccag ttttagatgg ttttgatcca tccacaatac gccccattgt
     1921 tatgtctgta actgcatcaa taaatcttgc tactaaaaat aatgttccta cggtagctcc
     1981 gcttatcccc aatacttttg tataaaaaat cattaaaaaa gaactagcaa aaatgaaagt
     2041 gaaatcattc ccaaaatctc caaacatata tcctattttg tctcttagac caaattttct
     2101 ttccatataa tttgcgctcc ttcctattgt taagcgtttt cattctgttg aactgccaac
     2161 ttacgtacta tactctgaag tataaagagt tagtatttat tttttacatt ttatagtatt
     2221 atttattttt tctcctaata attctttatc tcataaaact tatctatata tttactagct
     2281 attcgagtta cttcttcaaa atcacctttt tcaacaggag ccaacaaatt cccacctaca
     2341 ccaactgcaa caacgcctgc ttcaaaccac tcttctaagt tatctagact tactccgcca
     2401 gtaggcatta tatttacata tggtaatgga gcttgatacg tttttacaac agatggacca
     2461 aaagcagaac ctggaaatag tttaatgatt tctactccag cggtaagtgc ctcctttatc
     2521 tcagtaattg tcatacaacc tggtaaataa ggaatttgat ataaattaca aatctctgct
     2581 gtttgtcgat caaaagatgg acttacgata tattctgctc cagccataat tgcaatacga
     2641 gcagtaaacg catctaaaac tgtaccggct ccaattatta catctttttg atcagcatat
     2701 tttttagcaa tttctttgat tatttcttca gcattcggaa ctgtaaaagt tagttcaatc
     2761 cctttaattc cacccgcagt aattgcattt attgctttaa tggcctcatc tttatttttt
     2821 ccacgtacaa ccgcaatgac tccagccgct tctaacttac ttaaaatttc tacttttttc
     2881 atatctatct ccttctttta tggatacata ctctagctag ttgtactaa
//
LOCUS       DAPCMT010000202         2920 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPCMT010000202 DAPCMT010000000
VERSION     DAPCMT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.208"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            594..>2920
                     /gene="esp"
                     /locus_tag="RD743_002739"
     CDS             594..>2920
                     /gene="esp"
                     /locus_tag="RD743_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDU0564505.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1063 a          444 c          569 g          844 t
ORIGIN      
        1 ctttaaatat ggacaattca attataaaga aaaagaatca ataatttaat gaggctgtga
       61 caaatgtcat tttttgtttc gctaaaaacg agtgaaaaaa tgttgataac tccaatcatt
      121 cgtttaagat tctaggaatc attcagaaat gctgtgaaac cgatacttta taattataaa
      181 gaaggtgatt tcaaattccg aagaaggaat ttgaagtcac ctttttactt atgtcacagt
      241 ctcattcagt aatgcgggat aaggctcctt attccgttat ttgaatactc tgaagtttaa
      301 tctaagaaag ggtaatattt tgaaaacatg cttcatcgca tagaaatgaa cttaatttca
      361 gaaagaatac atacatatct ttttatatgt tttcagctgc tgaatggtta aaaagcacaa
      421 atatccgaaa aaaacatgaa gaaatgtact ttttttgttg taatgttcat gaaattctct
      481 aagtaaatta gttaacataa ggttagtaag attcctattc tgtgaataat acgtatcaga
      541 tttatctttt attcttacta ggaaagtaat tattaaggga gttgatttga ataatggtta
      601 gcaagaataa taagagagta tttctagaaa aaacaaagaa acgagtattg aagtacagta
      661 tcaaaaaatt aagtgttggg gttgcatctg ttttgattgg tgttggtctt gtcttgggaa
      721 caactgaact agttaaagca caagatgaaa tttctccaag cagcccactt gaaacagcga
      781 taagctccgt acaagtaggt gacaaagtag cttcggggaa tacttttcag gagaatcctg
      841 gttatacgaa aaattataac ttttcggatt tacaatttag tcctcaagaa ctaactggag
      901 acacactaaa aggcaataca attggatttg aagtttacgg aaaacataat attgcagcat
      961 caactaaaaa ctgggaaatc cgtcttcaat tagatgaacg attagctaaa tatgtagaaa
     1021 aaattcaggt cgatccaaag aaaggaattg gatcttctag acggaccttt gtaagaatta
     1081 atgattcgct gggtagacct acaaatattt ggaaggtcaa ttatattcgt gcaagtgatg
     1141 gattatttgc tggggcagaa acaaccgata cgcaaaccgc tcctaatggg gtaattacgt
     1201 ttgaaaagag cctagatgaa atttttaaag agataggcat agacaatctc aagaccgatc
     1261 gtttaatgta tcgaatctac ttggtaagtc atcaagacga tgataaaatt gtaccgggta
     1321 ttgatagtac gggctacttt ttaacagatt cagatgattt ctataatagc ttagatgtat
     1381 ctgaaaataa tcctgaccag tttaaacatg gatcagtaag tgcaaaatat gagaaaccta
     1441 atactcaaac aaaagatggt tcaggatcca caggtgctaa tggagctata atcttagatc
     1501 ataagttaac gaaaaattat aatttctcat attcatcttc cgcaaagggc acgccttggt
     1561 atgctaatta caaaattgat gaacgattag ttccatatgt agcgggtata cagatgcaca
     1621 tggttcaggc tgacaaagta acatatgatg tttcgtttga atccggaaca aaagtagctg
     1681 atttagcaat cgagcgacgg gaaggtcatg aaaattatgg cataggttca ataactaata
     1741 atgatttaac taaacttatc gattttgcta atgcaagtcc acgtccagtc gttattcgat
     1801 atgttttaca attgactaag ccattagatg aaatcttaga agatatgaaa gcaacagcac
     1861 aagttgaaga aaataaacca tttggtgaag atttcatctt tgattcttgg ttgtcggata
     1921 cgaataaaaa attaatccag aacacttatg gaacaggtta ttattatttg caagatattg
     1981 atggtgatgg aaaccctgac gataaagaag agagcggaga cacgaatcca tatatcggga
     2041 aacctgaatt agaagaagta tatgatgttg acacaacagt taaggggaaa gtattcatcc
     2101 acgagttagc gggaacaggt cacaaagccc aacttgttga taaagaaggt actgtattag
     2161 cagaaaaaac tatcgctcca aatgaaaaag atggggctcc aatttcagat actgtagaat
     2221 ttgaatttac gggtgtagat tcaagtaaat taatcgcgaa agatgaatta aaaatccaaa
     2281 tcgtttctcc aggttttgat aaaccagaag aaggttcaac cgttattaag gaatcaccaa
     2341 aagcggttga taaacaaacc gtggtagttg gatttaaacc agatgctaaa gaatcaattc
     2401 ggaataataa aaacttacct gaagatgcag agtattcatg gaaaacagag cctgatactt
     2461 ctaacgttac tgatagtacg aaaggtattg taactgttaa gatcggaaat cgaactttcg
     2521 acgtggatgt agagtttgct gtaaaagctt ctcaagctat ggaaaatgat gcaacatacg
     2581 tacctataac aacaactcca gaaacgacag ttcaaagtgg aaaacctaca tttgataaac
     2641 cagatgttcc tttagctaaa gatgcctttt caatttcaga tgtctataat aagtacttcg
     2701 gtaatgcaag tgttgatgca aacactggta ttgttacatt cactccagct aaaggtgtag
     2761 gagaatcgga gccgattact ggaacaattc ctattaaaat tgtttaccaa gatggttctg
     2821 taggcacgac cgatttagca gtaactgtaa gtaaagatat ttatgaaaat ccaggagaaa
     2881 acattcctgc aggctaccac aaagtaacct tcaccgcagg
//
LOCUS       DAPCMT010000203         2855 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPCMT010000203 DAPCMT010000000
VERSION     DAPCMT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.210"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..253
                     /locus_tag="RD743_002740"
     CDS             <1..253
                     /locus_tag="RD743_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0564506.1"
                     /translation="IAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGK
                     ISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            complement(936..1034)
                     /locus_tag="RD743_002741"
                     /pseudo
     CDS             complement(936..1034)
                     /locus_tag="RD743_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1299..1754)
                     /gene="tnpA"
                     /locus_tag="RD743_002742"
     CDS             complement(1299..1754)
                     /gene="tnpA"
                     /locus_tag="RD743_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDU0564507.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKIFRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1997..2855
                     /locus_tag="RD743_002743"
                     /pseudo
     CDS             1997..2855
                     /locus_tag="RD743_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          927 a          513 c          502 g          913 t
ORIGIN      
        1 aatagctaaa aaaaatggcg caaaaattat tgctataact aactatattc attcacctat
       61 aggaaagtct gcagacttag ttttacaaac agccattgaa gaatttctta atggtggatc
      121 tttagctgga aaaatatcac aactttatat ttgtgatcta ttagttcatg gttatgaaca
      181 aaacaataat attgattcag ttgaattaag agaaaaagtt ttgcgctcta ttattgataa
      241 aagtattgag tgaactaccc ccacttagct aaacctaacg gtttttaacg attgaagtag
      301 gcaattcttg ggatgagagc atacttgata gcttattttt tactaatagc ggtggctctt
      361 attgaccaaa tatccctgta gttcctacgg ttcttgattt tatctaaaga tatttttatc
      421 gtggaagcta ttgatcaact tgaaagaaaa acgtgtagct cattatcact agtttatcga
      481 gtatggatga agcaatggat catcctctct taaataaatt tattaaggat ctgatccttc
      541 aaattttttc catgatcgat gagcaggaac ctactgaatc taaacgacat cgggcacaag
      601 gaattattaa gattgctaag cgaatgtttt ctataaaggg tgttctaagc tatacagtgt
      661 taattcaaaa gattcccaat catcttgttt ataagaacaa cgttgaagat taaaaaagtg
      721 tttcaatgct aaaattgcaa aatacttcaa tgttatgaga caaactgtct ataagaatca
      781 atttccaccc atttcatcac ttgttaaaag tttatgatcc ttttgatttt tatattaatt
      841 tatctgtatt taatacagat tttatttatt agttcatctg cctattgtat tttattttgt
      901 tgccttacca tctgaagtaa atatactctt aattgtcaaa ttaagttaga cagatcatca
      961 atacttacgt aactcaaaaa tacttccaaa ggtgttcgat aatccagtga ttttctagga
     1021 agattatttc tgcgccagca ttttctaatg tttgaatttg tgttttgtaa tcttggttgc
     1081 ttgtgctaat tcttgaatag ccgtaaattg ccaaaatttc atcacccttt ctggacataa
     1141 gtttctgaca ccagttaaaa tcaagtctat cagtaaccca tacacccgtg aggacgggtg
     1201 tatttttaat ccccctagaa ggggtcgaca ctcgctgccc cttaaaaggg gatttttaaa
     1261 agaccgccaa ctgctcatct tcactcactt acttctcttt attttttaaa tggttctacg
     1321 tactctcttt tacttataat atatcgaatg cgatcatcag cttcttgttc acggatgtat
     1381 ttttcatttg tcttttgatt caatccgact gtactcacat agtatctctt cgaccaaaag
     1441 gatttgtttc cgtatttata tttgagattt gcgtgctgct cgtggattag taccgcacta
     1501 cgacctttca gataccccat aaaactggat atacatattt ttggtggtac tcgaacaagc
     1561 atgtgaatat gatctagcat tgcatgtgct tctatgattt ccacatcttt catttcgcac
     1621 aacttcctaa atatttttcc aagatctttt cgtaattgcc catagattac ttttcgttga
     1681 tattttggtg taaaaataat atgatactta caattccaac gagtatatgc taaactttta
     1741 tcgtcatttg acatgaaaaa actcctttcg atctgatgag cggttggtag ccactcacta
     1801 gtatatcgaa aggagttttt atcgctaaag atacttcttc cacgcgtaga aacgcgcggt
     1861 tttcttgttt cgcacgcttt acgcacaaaa ctgactgaag tcattaagaa aataaaaggg
     1921 tctaaaactt ttaagttttg taccgacccc caaaagttag accaaaaaat ctaacttttg
     1981 gaggtcggtg ttttttatgg ctaagtatac ttttgaactt aaattaaaga tcgttcatga
     2041 ttacttggat ggtaaaggcg gaagtgatta tctggcaaag aaatattcca ttaaggctcc
     2101 atcacaagtg aagcgctgga tcaatgccta tcaagaattt ggtgaagagg gtctagtaag
     2161 aaagcgacaa aagaaaaaat attctgttca atttaagctg gatgcgatag agttatatct
     2221 aacaagtgag ctgtcctatc gtgaagttgc caacacactt aacatgaaca accctaattt
     2281 gatcgctagt tggatgcgtc aattccgtga gggtgggatc gatggactct caaaaacgaa
     2341 aggatgtcca cctatcttgt ctaagaaaaa tgaacccaaa aataagaaaa aatctacaac
     2401 taaagcaacg tctaaagaac gtgaacgcat cgaggagttg gagaaacgag tccgttccct
     2461 tcaaattgaa aatgcctttt taaaagaatt gaggaagctg cgaaaacagg aagcccaaca
     2521 acgacgaatg aagcaatcgc acgaatcatc gcaagcctcc gaggaccgtt taaattagtt
     2581 gagcttctag aaactttaaa gttccctaag gcaatcttta tgtactggca aaaacgattg
     2641 gaccgtaaaa atccagatca agagattgaa gcggaaatgc ttaaagttcg ggagaagcac
     2701 aaggattatg gctgtttacg gatgaccaat gaactaagaa accgcggttt ctctatcaat
     2761 aaaaagaaag ttcagcgcct gatcaagaag ctaggaataa aagtaacgac atacacacgt
     2821 aaatcacggc gctacaattc ttatcgagga caagt
//
LOCUS       DAPCMT010000204         2848 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPCMT010000204 DAPCMT010000000
VERSION     DAPCMT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.211"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            212..901
                     /locus_tag="RD743_002744"
     CDS             212..901
                     /locus_tag="RD743_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDU0564508.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            915..2417
                     /locus_tag="RD743_002745"
     CDS             915..2417
                     /locus_tag="RD743_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0564509.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2430..>2848
                     /locus_tag="RD743_002746"
     CDS             2430..>2848
                     /locus_tag="RD743_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0564510.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFND"
BASE COUNT          920 a          396 c          552 g          980 t
ORIGIN      
        1 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
       61 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
      121 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
      181 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
      241 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
      301 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
      361 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
      421 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
      481 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
      541 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
      601 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
      661 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
      721 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
      781 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
      841 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
      901 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
      961 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     1021 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     1081 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     1141 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     1201 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     1261 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     1321 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     1381 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     1441 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     1501 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     1561 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     1621 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     1681 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     1741 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     1801 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     1861 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     1921 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     1981 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     2041 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     2101 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     2161 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     2221 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     2281 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     2341 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     2401 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     2461 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     2521 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     2581 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     2641 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     2701 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     2761 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     2821 ttccggagaa aaatcagttc aatgattt
//
LOCUS       DAPCMT010000205         2839 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPCMT010000205 DAPCMT010000000
VERSION     DAPCMT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.212"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..254)
                     /locus_tag="RD743_002747"
     CDS             complement(<1..254)
                     /locus_tag="RD743_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564511.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
     gene            complement(244..1443)
                     /locus_tag="RD743_002748"
     CDS             complement(244..1443)
                     /locus_tag="RD743_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDU0564512.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1573..>2839)
                     /locus_tag="RD743_002749"
     CDS             complement(1573..>2839)
                     /locus_tag="RD743_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0564513.1"
                     /translation="DATDRASKETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKK
                     ISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKE
                     TKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGAD
                     ETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVK
                     GDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGV
                     VVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTAD
                     KLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIF
                     MSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          866 a          552 c          429 g          992 t
ORIGIN      
        1 gtatcgactt ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac
       61 accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct
      121 tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga
      181 tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg
      241 tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa
      301 aagactgtag acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga
      361 gaacactccc ttttttgtga cttttctgaa gctagagtga atactttcaa ctgcattagt
      421 agtgtacatg atttttctaa tggcgctgcc ataatcaaag agttgttcaa catgagaaaa
      481 gtttcgtttg catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc
      541 gaaggcagcg tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca
      601 gacttcctta taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat
      661 acagcgctgc acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac
      721 accgtccatc gaaatgaaaa aaacatcttc tacaccacct actttcagtt catcaaatac
      781 ttgcatccag cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt
      841 tcctttaaga tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc
      901 gcgtaaagtg acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg
      961 agcttgccat tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg
     1021 ggaaatatca aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc
     1081 tcgtgcatac atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg
     1141 aatcaactca ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc
     1201 aaagctcgtt ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg
     1261 ctctttagaa tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc
     1321 aaataaggga ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg
     1381 ataagtattc aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc
     1441 catcgtgtaa tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc
     1501 gcctaggatt gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa
     1561 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
     1621 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
     1681 catgaattta ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc
     1741 ttctgcccca ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc
     1801 accagtaacc ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa
     1861 ataggaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
     1921 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
     1981 catccataag aataataata atgaaactac aactcctgca aatattaatg agccaaaaaa
     2041 taatttattt gaaattgagt aaatacctga tatagattgt tgtaatgcag ggtaatttga
     2101 tgaactttta atcaaattat attctctcca atttatatct aatttcccaa gatcttttat
     2161 tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt
     2221 atcttcggta ttaccataaa ctttagcagc tgtatgaata tcagtgatta atgtattttc
     2281 gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat
     2341 ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt
     2401 tatttttatc ttatctccaa ctttaaggtt atttttttca gctaaatctt tatgcattaa
     2461 gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga
     2521 tacaaatttt gtttcttttg ttgagtcgtt aaccccagtt aacataactg ttcttttaaa
     2581 attctttgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata
     2641 atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg
     2701 agatatcttt ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt
     2761 tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg tttcttttga
     2821 agctctgtcc gtagcatct
//
LOCUS       DAPCMT010000206         2729 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPCMT010000206 DAPCMT010000000
VERSION     DAPCMT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.213"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="RD743_002750"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="RD743_002750"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDU0564514.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1068 a          423 c          558 g          680 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacc
//
LOCUS       DAPCMT010000207         2665 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPCMT010000207 DAPCMT010000000
VERSION     DAPCMT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.214"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..181)
                     /locus_tag="RD743_002751"
     CDS             complement(<1..181)
                     /locus_tag="RD743_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDU0564515.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYN"
     gene            complement(300..755)
                     /locus_tag="RD743_002752"
     CDS             complement(300..755)
                     /locus_tag="RD743_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564516.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(1339..2142)
                     /locus_tag="RD743_002753"
     CDS             complement(1339..2142)
                     /locus_tag="RD743_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDU0564517.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT          837 a          506 c          328 g          994 t
ORIGIN      
        1 aattatagtt tcctaattga taaatgtagc tacccaagcc tgttaataaa aaaagcactc
       61 ctagaaaagt tgcaagcata atgcgtaatc cgtgacgtgt tctcttatcc ttatcaccaa
      121 gcaaccctcc aataatactg tattgatctt tggtagatgt tgaattgacg tttttttcca
      181 tcagtttgtc tgcccccttt cataaaagag caaacaatca gacgtttttt cggtaattct
      241 aggttcggga ttttgaatca tttcggtata ttcttcccca ttaatattta acacaaaaat
      301 tatcttcctt tcttcgttcc tttcttttgc tctttatttt ccaaataatc aatgattttt
      361 gctattgtac caagatagta attcccctca gctttgtttt ttcggatttt gctaaccatt
      421 ggagtaggta caatctcttc atctctcaat tcactgaaag attttttacc tagatatttt
      481 ccaataacag attttaaaaa acgttcaaaa tctttctcgt aagttgtttt cttgcctttt
      541 agttcatggt tcatattgtc aacgactttt gcggtttcaa taattgtttt agttctcagc
      601 ttaataattt catctggaat tttcaatcct cgtaatttca tcgttgtact aactggtaac
      661 ccatgcttta ctgcatcagc aatactaaaa ctttttagat catctagtaa gctctccata
      721 tagttaacaa attcatgcca attaatagca ttcattgtct caccccttca tttttttaaa
      781 atgaggttcg ggcgtaacac atttatgtta ttataatatc ataataacat aaataaatca
      841 atcagcaaat ttagatatcc cttaaagtat cagcaattat tcaacaagat atttatttta
      901 gctatttcag tcatcataaa agactaatta gcaataaaat aactaatatt atccatttat
      961 cagacgaaaa gaaaaaactt atctaattac tctatattaa cttttaacaa atagttgact
     1021 tgtccaaaaa tgttaccttc aatctcacaa agatattaag atgtttccta ccaaaactga
     1081 tatcaccgga ctttttattc tgtgttgtcc aaaagtgtta cctttagtcg atcaacaaat
     1141 taaaaaaaat tgtttttttc ttgacttgtc caaaagtgtt acccatagtt gttaaaattt
     1201 gtatagatta gccattcttt cgaatattat ttagactgaa acttgcttac aaaaaaactt
     1261 cttgcattta ttgtccaaaa gtgttaccca tactttttta aagaagcagg ttgtattaaa
     1321 attacaacct gcttcttctc aatcttcttc taaccaatta tgcattgaaa tggttggcaa
     1381 gtttgattct ggctcactaa aagtgaatct gagccgatca actcttcttc ctttctttgc
     1441 atagattttt tcataatcaa aagtttcaaa tacctgtatt ccgttttcat caggagctaa
     1501 aaattcttct ttcgcctgct ctagtattct tttgtcaata tttgtcattc tataacttga
     1561 aggtatactt aatgctcttc taaaatcttc aactttgact acccaataac catttcgcca
     1621 ctctttattt ctgtattgca tgagatgacg atacaattct tttgtgtagc tacttcttag
     1681 ttttgtaaaa ttatcaagtt caaaacgagt aaagtttttg ccaattgagt ttaagataaa
     1741 ttcaaattcc tcatttgcct taattgtaac tgtttccttc tgtttatcta tccggaattt
     1801 ttgaaaaagc cacagctgtt catagacgtc tgaatcttca aaacggtagg ttaaagtacc
     1861 tagtttgtca ctcaaagctt ttaacacaga ataaaaactg tccttttctc ttctgtcgta
     1921 tttgaccaaa taacgaaaga ggttgaaatc aaaagttact tctgctgttc cttgacgttt
     1981 catttttgat aaaatagccc aaaacacatc catttcaaca ggcttaaatc ctcgcaaagc
     2041 tgttgtattc agaccattgt tataacgcac aattgcattt gtttgttctg atactaaatc
     2101 tacatcacta tcaataatct tttcggtaac ttcttgagac attgattact ctccaatctt
     2161 ctttttgcgc tcctttaatc atgttaatct ggtactaatt aaaggaaaca attttttctg
     2221 cttgttacct aaattaaatc acaactaaag gtaacagtca acaaagattt gttactaatc
     2281 taattttcta ttttttcgcc ttccaaatca agaaaaccga aggtaacgtc ttatcctagt
     2341 gtgatacctc aaatgtccac ttttgttacc tttgctgtcc aaaagtgtta cccttactgt
     2401 ccacttttgt tacctttact gtccaaaagt gttaccttta taccccttta atacttactc
     2461 tcccaagaga ttaagcctcc cctaacagat atattaacag atatttaaca gatatattaa
     2521 cagatattta ataaaggagg attactcctc tcttatttca aaatcttaaa aataaaaaat
     2581 cttaaaaaaa actaacacta tctaggagtt tattttgctt gtgtaacttt gaataattga
     2641 aaaaaaggaa aaggaggaag cttca
//
LOCUS       DAPCMT010000208         2656 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPCMT010000208 DAPCMT010000000
VERSION     DAPCMT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.215"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            20..436
                     /locus_tag="RD743_002754"
     CDS             20..436
                     /locus_tag="RD743_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDU0564518.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            450..1016
                     /locus_tag="RD743_002755"
     CDS             450..1016
                     /locus_tag="RD743_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDU0564519.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1039..2025
                     /locus_tag="RD743_002756"
     CDS             1039..2025
                     /locus_tag="RD743_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDU0564520.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2180..>2656)
                     /gene="scm"
                     /locus_tag="RD743_002757"
     CDS             complement(2180..>2656)
                     /gene="scm"
                     /locus_tag="RD743_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDU0564521.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          795 a          492 c          572 g          797 t
ORIGIN      
        1 tggaaaaagg agaacaaaaa tgagccttaa agaatttgct gaaaaaagtc tgctcaaacc
       61 ttgggatgtt tttattattt tgcttttgac tgttctttct tttttaccag tcatgatttt
      121 tggtcttcag caaaacaccg caacagcaga aaaagaagcg gtactgcgtg tggatggcac
      181 agaaatcaaa acattccctt tgatggctgg agaagagagc aacacatata cttacaaaga
      241 tgaacacgga gattataact tgatcgagat agatggcgat cgagtacgga taaaagaagc
      301 agattgcgat gaccagatct gcgtacgtcg aggctgggca acgaaaaatg gcgaaaccat
      361 cgtctgtttg ccacataaat tagtgatcga agtccgttca acagatggag gtgacgaaga
      421 cagtctgatt tattagacct tcacacacca tgagtaaatt acaaaaaatg atttatattt
      481 cgctacttgt tgcacaagga gtgatcattg gtttattgga aaatatgatc ccttatcctt
      541 tttcttttgc gcctggtgca aaactaggct tagccaatct aatcacgatc gttgccattt
      601 ttaccatgaa aaaacaagac agcttcttac tactttggct acgactattc ctgactactt
      661 tgctaggcgg aacgatttcc acattccttt atagtatgag tggtgctttg cttagttatt
      721 ttggaatgtt attggtcaag caattaggac caaaacgtgt cagtatcatc ggaattagtg
      781 cggccggcgg tttcatgcat aatgtcggac agcttttgat ggcttctttc attgcacaat
      841 catggacagt catgttgtac ttgccagtcc tttcagtttt agggatttta tcagggattg
      901 caatcgggat cgcagcgaat tatcttttac gacatgtgca gacacttcaa cgattccaaa
      961 aagattatga gcagggaacc aaacaaaaat ggagcccaat gtttaccaaa aaataatcgt
     1021 cacacaagga gtttttttat gaagctacat ccaatgtggg aaaactatcc tgctttaagt
     1081 aaagaattga cagaaacact aaaacggatg gaagaaactg tccgcctgaa aaacaaacct
     1141 gtcgaacaag ccatcaaaga aacgatccat gccggcggaa agctgttgcg tcctgcctat
     1201 cagctattgt tctcacaatt tggaccagag caagatcgag acaaagccat cgctttggca
     1261 gcttctatcg aaatgctcca tacggccaca ctgatccatg atgatatcgt tgatgaagcg
     1321 gatctgcgac gtggtcagcc taatatccgt agtcagtttg gtaatgtcgt ttctgtctat
     1381 gcaggagact atttatttgt ctgctgtttt aaactattgt ctgattactc cacttccttg
     1441 aaaagtctcc aattgaattc tcgcagtatg gaaaaagtat tgaatggcga attagggcag
     1501 atggatgatc gctataatta tgaactttct gtaaaagaat atttggaaaa tatttcaggt
     1561 aagacagctg aactgttcca attaagttgt tctgttggcg cttttgaaag tggaacaagc
     1621 gagcgcttcg caaaaaaagc aggagatatc ggactttcta tcgggatggc ctttcagatt
     1681 atcgatgata ttttagatta tacaaaagag agccaggaaa tcggcaaacc tgtactagaa
     1741 gacatgcgtc aaggggtcta ttctttaccg ttgatctact ctttgcaaaa aaacaaacca
     1801 aagctactac cttatttaga gaagaaagcg gcaatgacag aagaagatgt cgacgccgta
     1861 cgcaaaatcg ttgaacatac aggaggcgtg gaagaagcta ggaagttagc ggcctcttac
     1921 acagaaaagg ctttgaagga aataaagaaa ttacctgcca cctctcttcg aacaaaagag
     1981 aatctctttt cccttaccca actgattttg gatcgcaaag actgaatagg gaaaggttcg
     2041 atacgtaaat aaatttctca gaaggaaatc tacgtgtttt ttgacaaatt gaattgtagt
     2101 tcttgtattt atgataaaaa gcaaaaataa agagcaacgt ccgttagcga cgttgctctt
     2161 ttttatcttg aatatttttt taccttttcg atttcttata actcataaaa gtgattctta
     2221 aaccgattac aagtaatata acgcctgcga gtgttatcca attattcgat tgacttccag
     2281 ttttaggaag tgctcttttt gatttgtttc cattgtcatt gcttgtttta ttagttaagt
     2341 taaagttcgt cctgttatcc gaacttgttt ttgagaagga gacacttgca tgacgtgtat
     2401 cgcttgtggt agacgacgtg tctgtactat cgcttgaagt tgatgaatct gagctatcac
     2461 ttgtggtaga ggatgtatct gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg
     2521 tagtggtaga tgaatctgag ctatcacttg tggtagaggt agtatctgta ctgtcacttg
     2581 aagctgacgt gtccgtgcta tcacttgttg tggtagatgt atccgtactg tcgcttgaag
     2641 ctgacgtgtc cgtgct
//
LOCUS       DAPCMT010000209         2646 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPCMT010000209 DAPCMT010000000
VERSION     DAPCMT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.216"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(28..273)
                     /locus_tag="RD743_002758"
     CDS             complement(28..273)
                     /locus_tag="RD743_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564522.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(267..668)
                     /locus_tag="RD743_002759"
     CDS             complement(267..668)
                     /locus_tag="RD743_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDU0564523.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(840..2633)
                     /gene="uidA"
                     /locus_tag="RD743_002760"
     CDS             complement(840..2633)
                     /gene="uidA"
                     /locus_tag="RD743_002760"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDU0564524.1"
                     /translation="MTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESYPISVP
                     SSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNGKLINQ
                     HKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVN
                     FDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDE
                     EAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGIRSIQV
                     SNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYS
                     EEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKMAIKEL
                     IERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPD
                     ICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTEYGADT
                     VAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILRVQGNK
                     KGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
BASE COUNT          793 a          510 c          351 g          992 t
ORIGIN      
        1 agaataagtg gaaaggggac tttttagtta gttaatttta aattttaaga tcgcttctat
       61 caatttaatt tctagctgtc ttagcatata ctcatctaca taggtaataa attgccccgc
      121 ctcattaaat ccatttgtca aacataactt agctatataa ctatgataat gtttgagtat
      181 catcttgatt gaaattggat cttcttctag agcagattgg ataatggata gaggaactaa
      241 tggatactgc cgttttttgg ggttgtctac cataagtcat accccatttt taattgttca
      301 actgccttct ttcgttgata ccagatacca cttgtactca tattgaggat tttaccgatt
      361 tcagtatcat taaaacctgc aaaataataa agtaggataa cctttcttct tatttccgat
      421 aaatgatcaa tggctgccga taacttttga tctgaaatta atatttccat tccaaataag
      481 aaatagacat cttgttcatc aaaatgagtt tgggagtact taccttcatt tgaattcgtc
      541 aatggttcct cattatctgt tatgttaatg tttcgttttc gaatagactt agtttcgttt
      601 cgtaatactt ttttacagta ggcatcaaat tgatgttgta tagatttctc attgtaattt
      661 tgttgcatga taaatatgct ccttttttcc cctttccact aatacaacaa tcagaaagcg
      721 cctttttttc gaaaaaacta gaaaaaaata tccttcttta aacagaaaat ctttttataa
      781 aaaaaaggag ttggcaatta agcagccact ccttttttat attccttata aaaggtaaat
      841 tattttttat aactgaaatt tggtattgat gaccatcgtt tctttagata gtgagcaatc
      901 attttaggct ttctatctct agtaaagatt cctttcttat ttccttgcac tcttaaaatc
      961 ccttgacttg tttcaaaatc tgcaaaattc cacacttgtt caccaacaaa attgtcacat
     1021 ttatctatta cttcgtgatt tgcttcgtaa taacccactt gatactcttc tgtaaacatt
     1081 gttggtactg tatcatgaag tccagctact gtatctgctc catattctgt aaacattatt
     1141 ggcgtatcgg gacatctttc catccatcca tctagctctt ttttcaacag atattttgca
     1201 tgttttaggt ctccacctga aacataccaa ccataatagc gatttaaaca taaaacatcg
     1261 acaatatctc ctactttaca aatatctggt gttgacttta agtaagtaac gattgttaca
     1321 ggtctcttct gagggtcaag ttctttagtc aattcaacta aaggttcaaa atattcttta
     1381 gcgccttcag aatccgaatc tggttcattt gcaatagacc acattacgac actcggatgg
     1441 ttcttgtcgc gttctatcaa ttccttaatc gccattttgt gagcattttc tgtccgtatt
     1501 tctttccaag tatcatgttt ttctacgttg tcatctaata aagtagccat gaaattcaaa
     1561 tgtaatccta ctgcaggtac ttcatcaata actacaatac cttctcgatc tgctaagcgc
     1621 ataatttctt ctgaataagg ataatgtgaa gtcctaaatg aattagcacc gatccatttc
     1681 attaaattaa aatctttgat atttactgct tcattcaaac cacgaccatt aatatacgtg
     1741 tcctcatgct tcccaaatcc cttaaagtaa aaaggtttat tattaatcaa aaacttccca
     1801 ttcgaaacct gaatactacg aatgccaaat tcttctgtgt acgtatcaat cactttattt
     1861 tcgttaatta aactaacttt catactatat aagtaagcat ttagaggctc ccaaagtgtt
     1921 gggttcttaa gttgaatcac tccttctttc cccttagctt ctgctataag ttgagcttct
     1981 tcgtcataga ttgctattag aatttctccg gtccattctg tatcgatatt aaattctaca
     2041 tttgcatctg aatcaattat ttttgtatta atagtgacat cctttatata gtctttcgga
     2101 gttgtataca atttaacggg tctattaatg cctgcataat tgaaaaagtc aaaattaacc
     2161 aaattttctt tttttatgtc tccatcttcc tgtatcgttt ctttcaaaat ccctacaggt
     2221 aaagtgctat cgtcaataac attgtttaca gccactgtaa ttctgttact tccatctttt
     2281 ataaactgat taatttctga ttcaaatggc gtaaaccctc ctttgtgttg attaatcaat
     2341 tttccattca aaaatacttt tgcttcatgt gttgcagatc caaaacgaag aactaatctt
     2401 tcttctaata attttttagg aagaatcact tctttttcat accacaccca tccaacatga
     2461 tttctaatgt tctctgtttc aaataaatca ttataagaag aaggaactga aattggataa
     2521 ctctcttttt caaccattgg ttctaaagca agttcttctt gtaagtcatc atttgtatct
     2581 aacttaaatc tccaaagacc gcttaaatca attacttgcc tcgtctctgt cataatggga
     2641 tataac
//
LOCUS       DAPCMT010000210         2612 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPCMT010000210 DAPCMT010000000
VERSION     DAPCMT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.217"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..275
                     /locus_tag="RD743_002761"
     CDS             <1..275
                     /locus_tag="RD743_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564525.1"
                     /translation="KIVDYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPN
                     EMWFEVTVESSDFKEKKKEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            600..1217
                     /locus_tag="RD743_002762"
     CDS             600..1217
                     /locus_tag="RD743_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0564526.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1258..1557
                     /locus_tag="RD743_002763"
     CDS             1258..1557
                     /locus_tag="RD743_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDU0564527.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1591..1758
                     /locus_tag="RD743_002764"
     CDS             1591..1758
                     /locus_tag="RD743_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDU0564528.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1803..2408)
                     /locus_tag="RD743_002765"
     CDS             complement(1803..2408)
                     /locus_tag="RD743_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0564529.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          913 a          402 c          477 g          820 t
ORIGIN      
        1 tcaagatagt agattacttt gcgaatactc ctgaagaaca aatacagaga aaacttcctg
       61 agtttgaaag agtaccacct tatgatccaa gagataattc atttcaagta aaaaaattaa
      121 aagagtctcc aaatgaaatg tggtttgaag ttacagttga atcaagcgac ttcaaagaaa
      181 aaaagaaaga atacaattat attgtaaagg caaaagtatc aaaaagattt atttttaacc
      241 ctaaattaga aatatatagt gttgtaatag aataatagtg ttaaaaaaat tgtaaaagtt
      301 aaagaagtca aattctgatg tgaaaaataa aatataccat ggaagacata aaagaaaaac
      361 tggataacta gtttatttaa tggcagaccg ttaaggcctg ccatttttat gtattttagc
      421 ggaataaata taatcatgcg ggagtgtaaa cttactgttt caaaaagtat actttttgac
      481 atgccttgat tttctttgtt tatgtatggc ataataacca cataatacaa tttgaaacgt
      541 ttctgattta atctgattgt tttgaaacaa ttttagtgaa aaaagggtag ataaaaacga
      601 tgagaaaatt tttttatatc cgggtcagta gttatgatca atcgatcgaa cgacaactga
      661 tttccgcgaa ggaattagga atccctgaag aatatatatt tgtagaaaaa gccagcggca
      721 aagattttaa acgccctgaa tatcaattaa tgaaacggat gtttcgaaaa ggagatttac
      781 tctatctaca atcattagat agactaggca gaaataaaca aatgatttta gaagaatggc
      841 gagaattaac taaggaaaag cagattgata tttctgtgtt agacatgcct ctgttagata
      901 ctgctagtta caagcaacta gatgggttag agacgcttat ctctgactta attctgcaat
      961 tactcagcta tatggccgaa gacgagcgga aacgaattaa tagccggcaa agagaaggcg
     1021 tagaagctgc gaaacaaaag ggtgtaaaat tcggcagaaa gaaaattgct ttacctcctg
     1081 aatttcctgc gatctattct gactggaaag caggaaagat aactgcagtc tatgctatgg
     1141 atcaattggg catgaaacga aatacctttt atcgacgggt gaaagaatat gaacatacct
     1201 tgcaccactt ttcttgatag atataacagc tattagacgg gaagggagag tttgaaaatg
     1261 gacttaacga atcaaagggt cgtttttact ggaacgctgc agcaaatgac acgaacccaa
     1321 gccatcggtt tggttcactc tttaaacggc cattgtcaat caagtgttac ttcaaaaaca
     1381 aatttcctgg tttgtggtca gtattctaag tcgttgtttg atgattctct ctatacaaaa
     1441 aaggaacaaa ccgcgcggga cttgattgcg ttaggtcatg agattaccat tttatcagaa
     1501 gtcgaatttt atgcgttaat tagtcaacag ttgaaagaat ggcaacggta tttataatcg
     1561 ttagtaaaat ttacaaggag gaaaattttt atgaagcgaa aatcttatga tcgttggggc
     1621 attgaaataa ctagttttaa tcgaaatatt gcttttcacg tggagtgtga ctgtggggaa
     1681 ttagcaaaaa gtgtttatca aaaaaattac ttttactgtc ctaaatgcca tcgccgatac
     1741 ggggtacatg gaacatagaa acgaaatagt gcggtagaaa agcgaatgaa tgtagtatat
     1801 ttctacaaat cattcgcttt ctttatttta taaatagttg gtcgagagat cccgttttct
     1861 tttgcaatct ctgcaactgg aatgccttgc tttaacatgt ccaccacggt tttaaaaact
     1921 aagcgttttt gcggatctgt tgagttaata gaatactcga ttggtcgtcc cttatatcgg
     1981 ccggcttttt gagccaataa tactccttgc cgctgacgtt cacgaatttt ctcacgctcg
     2041 ttttgtgcaa tataacttag cagtgataaa aacatatcga acatagcggt atccaggagt
     2101 tcatttccag tattaaaatc taaaaactgt gcatctaata tttttaacgc tacttttttc
     2161 tgtttcataa aagcaactgt attttttata tcttcataat ctcttcctaa acgatctagt
     2221 gatgtaacga tgacgcaatc accttcacgg atataattta gtaatttttg aaactcagga
     2281 cgctcagtat ctttaccact ggctttttct tggaataatt tatcaatgtt aagtccttcc
     2341 atcattgcca attgtcgatc caaattttga tcttgtgaag atactcgaat atagccaatt
     2401 tttcccataa aaaactcctt tttaaaagtg atataattta catgtaaaat ttacttgtag
     2461 ttttactgct atcatagcat ttttaaaaat aaattttatg tagacttaaa atttacttca
     2521 tggagggttt aaatggatga aatcaaaagt aacaaaaaag catttggcat ttatttagga
     2581 gaacctagta cagaacaatt aaaccaattt tt
//
LOCUS       DAPCMT010000211         2586 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPCMT010000211 DAPCMT010000000
VERSION     DAPCMT010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.218"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(841..1359)
                     /locus_tag="RD743_002766"
     CDS             complement(841..1359)
                     /locus_tag="RD743_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDU0564530.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2477..>2586
                     /locus_tag="RD743_002767"
     CDS             2477..>2586
                     /locus_tag="RD743_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564531.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          852 a          472 c          387 g          875 t
ORIGIN      
        1 tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt tgttgacgat
       61 tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac ttatgaaatg
      121 aaagaccttt tacatcttgc agaaatcggt actgggatta aactaagcaa taaaaactaa
      181 tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata aatgacgatt
      241 tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc agtatcagta
      301 ataggatagg cgataggcca catacccttt gatggttaga ataaactgct caaaatctgt
      361 atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa ccataagcgt
      421 tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata taattaccgg
      481 acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa ttgtcggtac
      541 tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct agtctcctaa
      601 gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt catggtttat
      661 ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca ttctagggga
      721 gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg tagtcgtatg
      781 ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt aaacgggaat
      841 ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa atggagcaac
      901 tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg cctcgtccgc
      961 ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa tcatcatgtt
     1021 aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta gatgtgcata
     1081 ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt gatcagaagg
     1141 cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta aattcgtcca
     1201 accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa ctcttttatc
     1261 cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag tcctatttat
     1321 ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc cataaattcc
     1381 taattgtctc ctttaattca tctataattt catccacgat attttaatcc gttctacaat
     1441 gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa aatatcccaa
     1501 cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga atatatttct
     1561 tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca aaatcatgtt
     1621 aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg ttaagtttag
     1681 ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt aaaactcaaa
     1741 atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg taaaaaatat
     1801 tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa acagctacaa
     1861 aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc atattcacgc
     1921 ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt ttaggaaatt
     1981 gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc accagatatt
     2041 cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca ccgcgaattt
     2101 actaaaatta tgaataatta tgttaatctt ccgaaatttt tttccaaaat taagggatca
     2161 agctggtgtt atagattgtt taaggttggc aaaaaaataa cttcttttta aacaattata
     2221 gaaaaccgat actctgttaa gttggttgac acggtgtcga tttttatgac tttcttattc
     2281 tgaaacttca ctaacggaca gtacctaaaa agtctatagt atggacgttt acgagtacta
     2341 ttttatcaaa aatgctgctc atttcaactt atatgtttta gtgaactacc cccacttagc
     2401 ttggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag aagaaaacat
     2461 accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag aattcaagca
     2521 acaaatcgtc aatctttact tagctggaaa gccacgtgta gaaatcattc gagaatatga
     2581 actaac
//
LOCUS       DAPCMT010000212         2562 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPCMT010000212 DAPCMT010000000
VERSION     DAPCMT010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.219"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..1042
                     /locus_tag="RD743_002768"
     CDS             <1..1042
                     /locus_tag="RD743_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDU0564532.1"
                     /translation="NQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLI
                     GFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINS
                     TADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYG
                     NTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGP
                     ILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVG
                     KDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINISYIKQLCYRGRN
                     IISMIKSREHKR"
     gene            1078..1794
                     /locus_tag="RD743_002769"
     CDS             1078..1794
                     /locus_tag="RD743_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDU0564533.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            1813..>2562
                     /locus_tag="RD743_002770"
     CDS             1813..>2562
                     /locus_tag="RD743_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HDU0564534.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKK"
BASE COUNT          929 a          305 c          491 g          837 t
ORIGIN      
        1 gaatcaattt ctaatacaat ttgctaaagg gttagaaaaa gttaaagcta tgggcatagc
       61 aggtattatc agcacaattg taacaataat tgccaatatt ttcttcttgc ttattctgaa
      121 atggggatta attggttttt tcattgcaaa tattttatca cagattattt cagctactta
      181 tctgtcagta ttagtgagaa tatgggaata tatcgatttt gaggctaaag atcctcagct
      241 aaagagagaa atgctaaatt actcgattcc attaattgca actgttgttg gatggtggat
      301 taatagtaca gctgataggt atgtggtttc ctttatagta ggtgtctcgg cgaatggctt
      361 attgtctgtg gcatataaaa tacctcaaat aattaatact ttgcagggaa tatttataca
      421 ggcctggcaa atttctgcaa ttaaagaata tggagataat gagactgcaa aattttatgg
      481 aaatacattt gttatgatta atttgttaat gtgcataacc tgttcaagtc ttatcatatt
      541 aactagaccg ttttcacata ttctttatgc gaaagacttt tatgaagcgt ggaaatatgt
      601 tccattttta ttaatagcta atgttttaaa ttgtgcatca ggtctgttag gacctatctt
      661 atcagcaaaa aaagattcta aatccatgat gtggtctgca atcatagggg cattagttaa
      721 tgtgattatg agtgtagtcc ttgtttataa gataggggtt caaggtgcca caatagccac
      781 tgttatatgt tcatatgtaa tatatgttgt tcgtaaacga gctgtgggaa aagatatttt
      841 tataaagcat tatcatattg ttgtggaatc ttggggatta ttgtttgttc aggcaatcat
      901 agaaatatat tttaaaaatt atgtaataga aattataatt atgattattt taattattat
      961 aaatatcagt tatattaagc agttgtgtta tagaggaaga aacattattt caatgattaa
     1021 gagtcgagag cacaaacggt agacgatgtt atttttataa aaagatataa tttattaatg
     1081 gtcgataatt atgaaaggaa aaatattctt aaaaaaaaga gtataggatg tcgtatgaaa
     1141 atagtcgcta tagtaccaat gaagttaaat aacaaaagat tgccacaaaa aaacacgaaa
     1201 gcttttacca atggaaaacc attgtgttat tatatacttt caacactttt aaaagtaaaa
     1261 cagattgata aagtgtatgt ctattgtagt aacccagata ttaaagaatt tataccagaa
     1321 ggtgtaacat ttttaaagag atctagttcc ttagatcagg atacaacgag tattacacag
     1381 gtattagccg gtttcacaaa agaagtagag gctgatgtat atgtaatgac acacacaaca
     1441 gcaccattta ttagcgaaaa atctattgaa aaaggactag atgccgtgct gacaggagaa
     1501 tatgattctg catttgctgc aaaaaaattg caagatttct tatggaaaga cggtgaacca
     1561 tttaattatg aattggacaa tattcctaga acacaggatt tgccgccttt atacgaagaa
     1621 actagtggat tttatattta taatcgcaat gtgatgaccg agttgcatag aagaattggg
     1681 aaaaaacctt gcatagttga agtaggtgag attgagtcag ttgatattga tgaagccgag
     1741 gattttatga ttgctgatgc gatttataat catcttattt tacaaggtag ttaagacagg
     1801 gggaaggtta ggatgaacaa tgtacagatt ttagattgca cacttagaga tgggggctat
     1861 tgtaacaatt gggaatttgg atttgataat acaaaaaaaa ttatttctgg acttatagaa
     1921 tcaggaattg agattataga atgtgggttt ctcacaaata aaacagtata caataaaaat
     1981 gttacaaaat atacgacctt agaagaatta gcacagataa ttcctgaaaa aagagaagga
     2041 aaattatttg tagcaatgat gaactatggc gaatataata tagaggatct tccagattat
     2101 gatggttcat ctattgatgg aattaggatt gcgtttcata aaaaaaattg caatgaagcg
     2161 ttgaaattat gtcgtgagat aaagaaaaaa ggctacttag tttttgttca agctatggta
     2221 tctctcaatt atacagatga agagttttta gcagtaataa aaaatgtgaa tcagtttaaa
     2281 ccatacgctt tttatattgt tgatagtttt ggaatgatga aaggaaaaga tttaactcgt
     2341 cttttttaca tgattgagca taatctagaa aaagatatac agataggttt tcactcacat
     2401 aataacatgc agcttgcata ttctaatgca caaaaactta taaccgtaca aacaaatcga
     2461 agcctgatta ttgattcttc ggtgtacgga atgggaagag gagcaggtaa tcttaacaca
     2521 gaactatttg tagaatattt gaatgataat gcaggaaaaa aa
//
LOCUS       DAPCMT010000213         2558 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPCMT010000213 DAPCMT010000000
VERSION     DAPCMT010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.220"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..547
                     /locus_tag="RD743_002771"
     CDS             <1..547
                     /locus_tag="RD743_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HDU0564535.1"
                     /translation="KYVLQVFHPVVTELENIEKQILALINSMEQQSQPIICILPNSDA
                     GSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVIN
                     LGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITK
                     TLNQILNDGEDIKWIQKQIAY"
     gene            520..1197
                     /locus_tag="RD743_002772"
     CDS             520..1197
                     /locus_tag="RD743_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDU0564536.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1187..2329
                     /locus_tag="RD743_002773"
     CDS             1187..2329
                     /locus_tag="RD743_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDU0564537.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2342..>2558
                     /locus_tag="RD743_002774"
     CDS             2342..>2558
                     /locus_tag="RD743_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0564538.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPI"
BASE COUNT          952 a          337 c          502 g          767 t
ORIGIN      
        1 aaaatacgtt ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca
       61 aattttagct ttgatcaatt caatggaaca acaatctcaa ccaattattt gcattttacc
      121 taatagtgat gctggtagcg aaattatcag aaaaatgtac cagaaatatt taaaaacgga
      181 tattatttat attacgaatt tagagccgga ggaatacctg actatattaa aagagtgcaa
      241 atttatgatc ggaaattctt cttctgggat aattgaagct gcatcattca aaataccagt
      301 aataaattta ggaaccagac aaaatggacg agaacaatct aaaaatatta taaatgcaaa
      361 tctaaatgaa aatgaaattg aaactgcttt gaataaaata caagatcctc agtttataaa
      421 aagcttgaaa aatgttaaga acatctacgg tgacggcaac agttctagaa gaataacaaa
      481 aacattgaat caaatattga atgatgggga agatattaag tggattcaaa aacaaatagc
      541 ttattaaaat atactagtga tccatacatt attattgggg ctggcggaca cgcaaaagtc
      601 attattgaaa ccttaagatt agaaaataag ttaatatatg gattttttga tgataagcaa
      661 aataaaatgc aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat
      721 agcaaaaaaa aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag
      781 attataaaaa aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct
      841 atagtggcaa aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt
      901 aacccagatg ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat
      961 gattgtagaa ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta
     1021 catgtaggtg taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt
     1081 ggatcttggt gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc
     1141 ttagcttatg gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat
     1201 tttattatca agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa
     1261 agataattgg atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa
     1321 atatactggt tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct
     1381 tgccttaaga ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt
     1441 tatagctaca gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga
     1501 acctaataca tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag
     1561 aaacaaccaa ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta
     1621 tgaacgaatt ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga
     1681 atcgttaggt tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta
     1741 ttcatttaac ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga
     1801 taaaaaatat gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta
     1861 ttatcaacat agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg
     1921 acgtggacaa atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg
     1981 atataaggaa gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat
     2041 ggtgccaaat cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt
     2101 agttaacgta ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc
     2161 actacatttg caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag
     2221 tgtgtcagaa aagttattta atagcggctt atgcttacct tctggaagta atatgacaga
     2281 tagtgaacaa gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt
     2341 tatgaacata ggaattattg gtttaggttc catggggaaa agaagactta gactcattca
     2401 agaacacttt aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga
     2461 agttgaaaag gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt
     2521 tgaaataaat tcagtttttg tttgtacatc ccctataa
//
LOCUS       DAPCMT010000214         2532 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPCMT010000214 DAPCMT010000000
VERSION     DAPCMT010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.221"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..379)
                     /locus_tag="RD743_002775"
     CDS             complement(<1..379)
                     /locus_tag="RD743_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDU0564539.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            598..1968
                     /locus_tag="RD743_002776"
     CDS             598..1968
                     /locus_tag="RD743_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HDU0564540.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1989..2273
                     /locus_tag="RD743_002777"
     CDS             1989..2273
                     /locus_tag="RD743_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564541.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          859 a          380 c          522 g          771 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac at
//
LOCUS       DAPCMT010000215         2523 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPCMT010000215 DAPCMT010000000
VERSION     DAPCMT010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.222"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            6..2378
                     /locus_tag="RD743_002778"
     CDS             6..2378
                     /locus_tag="RD743_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564542.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         1010 a          399 c          555 g          559 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg cttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaacg aacaaattga gaagatgttt gaacaccaga
      121 gaaagaccaa cggtcagcca atgaacgata agagaaatgg gcattcttgg tttaggcaac
      181 aagaacgttt agaaaacaaa gtgcatagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggctataac aagtacggcg
      301 gcctggatat gacaatcgaa aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gccgctacga tccgaaaata ccaacggaaa ttggaaacat
      421 tagaacagct gaaagaaaga tcagaaaaag gaaaagaaaa tcttcttcct gaagtacaag
      481 caattattga cagcggacga gtgacgcaat ggaaaaaaaa tccaaccatt tattttttga
      541 aagactatcg aaaagttgct ttagaactga atgaaaacgg gggatttgag gaatcaaaaa
      601 aataccaagc cacgtcggaa gaggaaaaag cagctgttcg tgaattactt gaagaggctg
      661 cgccaactga ttctttgaag gagaaagaaa acgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga gatcgtaaca ttagaattga
      781 tcgatcaact caaaacgttg gataaccaat acgccgcaga agcaggttat tataaattct
      841 attttgaaga agtgaagaac ggagaagtag tcgatcagat ccgaatggat ctaggggatg
      901 gcgtcaacag taatcaaccc atctatttag agttagcaaa attgtgcgaa caagtacctg
      961 aaaaggaaca aaaacaaacg gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga
     1021 aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac ggcagaagag attaagcaag
     1081 aagtcaaatc cctttcgcaa caggcactgg atgcggtgaa aaagcatacc cattctcctg
     1141 aagacgtgaa agaacttttg aattttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gctgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgag aaaggacgtg gctttgttcg agaaacagaa gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaacac agttaaaacc tgaagactat ccgaaaatct
     1501 ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg acagtcagaa cttcaagctg
     1561 ggattcaagc ggtagccaac aatattggaa tacaaattcg agatatgaca gaaagtgaag
     1621 tctatcaaag agaattagga caagcacggg gggcatatgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagct acaagtattc
     1741 atgaattagc ccatgcaaga atgcacaaat tttcagaatt agataccgca actaaagaat
     1801 tgcaggcaga aatgacttct tatattgtgt gcaaacattt tggaatggac acttcggaga
     1861 aagctattcc ttatattgca gcatggacga agaatggtca gacgttggat gataaggaag
     1921 ctgcggaacg tgggaaaatc atgaatgatg tcagccgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccaa ccagcagaag
     2041 tgaagcagga gcataaaaat actgtagaaa cggcagaaca aaagaatatt gttcctgttg
     2101 gttcactttg gatcgacaag aaagacggga aagtaatgga agaagatacg ggtcgaacgt
     2161 tacatttgaa ggatgcggcg tttattgaag atccacagca taatcaagta attttaaaag
     2221 gaaaaagtta tggaaaagag atccaaacgg cgttaccttc agaagaattt gcgctaggaa
     2281 attcattccg attttcactt gagacagtgt cagaagtggc aaaacgaaaa aatgaaatct
     2341 attatgaaca acaattgtca caaaaagaac gtgtataaaa caagtaaaaa agactatctg
     2401 aaaaacggat agtctttttt cttttgggag gttttaaaat gaccgagaaa aaaatgagtc
     2461 ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat ggaatggctg
     2521 tag
//
LOCUS       DAPCMT010000216         2493 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPCMT010000216 DAPCMT010000000
VERSION     DAPCMT010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.223"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            189..2342
                     /locus_tag="RD743_002779"
     CDS             189..2342
                     /locus_tag="RD743_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDU0564543.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          857 a          428 c          548 g          660 t
ORIGIN      
        1 aaaaataagt ttatttttta atgaaaaaaa tagttggata tgtaacttta agtgattgct
       61 gtgaaacaaa aagttgattt tatcttgaaa ttaggtaaag aatttcttgc tccgcccatt
      121 ttttcttttt tattaagaag tttttgggta caatgaagca tgaatgtaaa taggcggaag
      181 gataggaaat gatgaaagat tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa
      241 taagaaaagg tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat gttccttttc
      301 gcttaaattt tctttttttc attatattta cgttatttgt tgcgttgatt gctagattgg
      361 gttatttgca gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct acttctacga
      421 ttacggtcca agaaagtact cctcgcggaa tgatctacga ctccacagga aaagctttag
      481 ttacgaataa agcgaatcag gccatcacat atacgagagg aacacaagtt acggctaaag
      541 atttgcttga aatcgcaacg aagctgaact cgctgatcga tgttccggta gatgaaaatc
      601 tgacagatag agataaaaaa gatttttggc tggctgatcc caaacatttg aaagaagcca
      661 caaaaagact ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat agtgcgatgg
      721 tcaataaagt cacagctgat gagattaatt taaacgagga gcaattaaaa attgctacca
      781 tctttaaacg gatgaatagt gcatatgaat taaacactgt ctatatcaaa aactctggtg
      841 ttactgatca agagttagct gttgtggcag aacgcgcttc tgaacttcca ggagtttcga
      901 ctggcacaga ctggacacga gaatataatg cagcaagttc actgaaaagt attcttggca
      961 gtgtgacgac tgaaaagcaa ggacttccag cagatgaagc agagaaatac ctagcaaaag
     1021 gttattctcg gaatgaccgt gtaggacaaa gttatttgga aaaacaatat gaagatgtcc
     1081 tgcaaggaac caaaacccaa tatgaagttt cccttgataa tgaaggaaat gtttcgaatc
     1141 aaaaagaaat cttctctggg gaaaaaggtt ctaatttaat gttatctatg aatgcagaat
     1201 tccaatcaaa agtagaagaa attttgaaaa ggaattatca aacattgatc aacaacggca
     1261 aagcacagta ttcgcctggt gcttatgcag tggctatgaa tcctcagaca ggagaagtgc
     1321 tagcaatgac tggtttcagt catgaacaag gttccaaaga aatcaccgag aatgctttag
     1381 gcacgattac tagcgccttt gatccagggt ctgtagtcaa ggctggtact ttgacagcag
     1441 gctgggcaag taacgcgatc agcggaaatc aagttttgat tgatgagccg attcgtctgc
     1501 aaggtgcttc agaaaaaagc tctgtcttta atcgaagcgg acaagttgct cttgatgcag
     1561 tcaaagcatc ggaattgtct tctaatacgt atatgatcaa agtggcgtta aagatgttag
     1621 gactagacta tacacctgga atgggactac ctagtttaga tgaagaagct aaagcttatc
     1681 agcaattgag agactctttc aaagagtttg gtcttggtac aacgactggg atcgatttgc
     1741 cgaatgaatc tcctgggatc tcacgttctg ttgattatat gaagaagttc aatgcggata
     1801 atggcaaaga atggtatgct ccaggtaact ttaccgattt ggcatttgga cagtttgaca
     1861 catatacacc aatccaatta gctcaatatg cttcaacagt cgctaatggc gggaaacgag
     1921 tacagccgca cctagtcaaa gcgatctatg gcaatgacga aaatggaaac ttgggtgaag
     1981 tgaagaaaga aattggcaca acagtagaaa atacggtcaa tatttctgca gaaaatatgg
     2041 cgatcctccg tgaaggattc caccaagttg tccatggtac cgatccttac acaacagcca
     2101 aaccattagc cagtgcgaaa atggaccttt ccgcaaaaac agggactgca gaaacggttg
     2161 cggaaggtca tccagatatt gcgacagtca atagtaatat cgtggcatac ggaccaacag
     2221 acaatccgga aattgccatc agtgttgtct tgccgaactt attagacgaa caagaccaca
     2281 taaacttgac gattgctaaa gaaataatgg acgcgtatta tgatatgttc atggcaaaat
     2341 aaaaaagagg tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat
     2401 agcttctcac atttttgctt aaatttgact tcataccgag aaactggctt ttgaacaccg
     2461 tttactcggc aaaaaaggtt gtgacaaaaa ttg
//
LOCUS       DAPCMT010000217         2426 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPCMT010000217 DAPCMT010000000
VERSION     DAPCMT010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.226"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            27..1361
                     /locus_tag="RD743_002780"
     CDS             27..1361
                     /locus_tag="RD743_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564544.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1348..>2426
                     /locus_tag="RD743_002781"
     CDS             1348..>2426
                     /locus_tag="RD743_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564545.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFF"
BASE COUNT          805 a          369 c          415 g          837 t
ORIGIN      
        1 attatggaga aaagtttttt aaaaagatag atgctgcttt tttatttagt tgtttatata
       61 cttttgcaac ttatcgaaca gttgatatct attatcgatc cgcaattgca gaagcaatcg
      121 ctcttacgat tatcccagct gttttatatt atgcttatgg attgatattc aatagagaaa
      181 aaaaagtcat cgctttatct gtcagtataa gtctacttgt gtatactcat gtcttaacta
      241 cactattgtg tagtgcattg attgcttgta tgattatagt tcgtttgttt ttaagcaagc
      301 aaataaaaaa cgatataaaa tttgtggtcg tgaatttagt aaaggctgtt tttttgactt
      361 gtgcattaac tagctatttt tggtatccta tgttgcagca gatgaattat caagaaatac
      421 acagtccctt caaaacagat ttgcagctag aagcactttc tatttcagat agtatcattc
      481 aagcgttaaa taacgatatt acaacataca ctatggggtt attaggattg gttgcgttga
      541 tttttccttt attcatactg aaagaaatgg agaaaaaaga aaaaatcatc tatgttggcg
      601 tagtttctac atgggtatgc gtaactaatt tatttccatg gtttttgttt caaaaaacac
      661 cgataagctt actccagttt ccttggcgta tcttaggtat ccaaatcttt ttttcttcaa
      721 tgatcataac gcttgttttt atgaaaaaga ctatgaataa aaaatattca tggttaatca
      781 ttggcgctac tgtggtgttt ctactcgtca cgtctatagc agcaaaagaa aattacgcaa
      841 aagttatcca atcaaaacac ggtcatattg ttatcaatga agaaacactc ccacgttata
      901 caacatcaga tatgggagga ttatacgatt acgccccaac agaagcgata aaagaaaggc
      961 ctcacttaga aaaacatgaa gtaaaagtag gagaatcgtg ggaaaaaggg aagtataaag
     1021 ctgcagataa taacattact tacacagtgg caagcaacaa aaagcaaaaa gtgacagtac
     1081 cagtatatgc ttatttaggt acacaggtaa aagtaaacgg taaagtcgta aatgactctt
     1141 ataaaaaaca tggcttagtg aatgtggatg taaatgctgg agaaaataag atagaaatta
     1201 caacaaaata tactccaact gctctcatgg ctttatggat cagtattctg acttattgtt
     1261 tagtggtgta ttcaagtttt aaacgatttg cacctaataa gttgaaagat tctcgatatg
     1321 tgactaaaaa ggagataaac aagaagaatg aaaataaata aaaaagtagt aatattgagt
     1381 ggattaatta tagttagtag tatatattct ttcatctttt tcaaaaatac acagagtatt
     1441 tataccactg gagatctttc ttttcactta tctcgaatca aggggttatc gactattttt
     1501 accagtccga ttaattatga gacattcaat tatactggat atggtgtaaa ttatttttat
     1561 ccctttctga ctttttttcc agctgtaatg ttatattgga tgacaaaaaa tttgattgta
     1621 agctatatta tttacgtttg gttattgaac ttgtgtacaa cactcgtagc ttatcattat
     1681 ggcgagcggt ttttaaaaca aaaaaaagcg gcatttttat ttagttgtct ctatattttc
     1741 tctgcttatc ggacagtaga catctactat cgttccgcaa ttgccgaagc tatcgcaata
     1801 acacttgtta tccctgtatt attttatgct tatcaaatca tctctggaaa agaagaaaag
     1861 tatccgtctg taaagctagc attgagtatg agtttgctag tgtacagcca tgtactatca
     1921 acattaatga gtacagcatt aattatcatt tttattttta ttcgtttggt ttctaaaggt
     1981 ttcaagaatg cagattttat tgctatattt aaaaaattat ttagtgcggc aggaatgaca
     2041 ttggttctga cgagtgcttt ttggtatccg atgtttgaac aaatgctcta ccaaaaaatc
     2101 aacaaaccta gcgtgacaaa tttatatgct catgcatcta atgtgttcga tagtctgact
     2161 gaagctatga acaacgactt aactacttac tcaatgggac tagttggtct gttatcttta
     2221 tgtattcccc tgattttatt taaaaaatta acaaacatag aaaaaaagat ttattatggt
     2281 acgtgtttga cttggctagc tacaactagt ttggtgccat ggtatcttct gcaaaatacg
     2341 ccagccaaat tgttacaatt tccttggcgg atcttaagct tgcaaataat attctcttct
     2401 ctgatattaa cgatgatatt tttcaa
//
LOCUS       DAPCMT010000218         2407 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPCMT010000218 DAPCMT010000000
VERSION     DAPCMT010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.227"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            5..1150
                     /locus_tag="RD743_002782"
     CDS             5..1150
                     /locus_tag="RD743_002782"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDU0564546.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="RD743_002783"
     CDS             1321..1779
                     /locus_tag="RD743_002783"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDU0564547.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="RD743_002784"
     CDS             1938..>2407
                     /locus_tag="RD743_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDU0564548.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAPCMT010000219         2400 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPCMT010000219 DAPCMT010000000
VERSION     DAPCMT010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.228"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            207..2069
                     /locus_tag="RD743_002785"
     CDS             207..2069
                     /locus_tag="RD743_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0564549.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="RD743_002786"
     CDS             2069..>2400
                     /locus_tag="RD743_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0564550.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAPCMT010000220         2400 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPCMT010000220 DAPCMT010000000
VERSION     DAPCMT010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.229"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(196..2280)
                     /gene="cadA"
                     /locus_tag="RD743_002787"
     CDS             complement(196..2280)
                     /gene="cadA"
                     /locus_tag="RD743_002787"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDU0564551.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          695 a          531 c          363 g          811 t
ORIGIN      
        1 cttttattta atacacatcg ttttttcata ttttttccat caaaaaaata agttattttc
       61 ttttatttca ttgaccttat tgaatttcaa gctattttga attttctgca aaattaaaaa
      121 cattagttat tttataatcc tatctatttt ctttacattt ttcattaaga aaaggatata
      181 aaaaaaagag gacgttcatt cgtcctcttt ttttacatgc aagcttcgca tggcattcaa
      241 aacagctaat actgtcaccc caacatctgc aaatactgca tctcccattg atgcaaaacc
      301 aaaagcacct aaggcaagta ccaaaatttt tatcccaata gcaaagacaa tgttttgttt
      361 gacgattttc aatgttttct ttgctaaatg gattgcttct gcaatcttcg atggttgatc
      421 attcatgatg actacatctg ctgcttcaat tgcagcatcg cttcccaatc cacccattgc
      481 gattcctaca tctgctcttg ctagcaccgg tgcatcgttg attccatctc cgacaaatgc
      541 ggtcttttta ttgtttcttt gtaagatttc ctctaaccgc tgcactttat cttcaggaag
      601 caattcgcta tatatcttgt ccacgcctac ttgcttcccg atatgatcag caattttttt
      661 cgagtcgccc gtcaacataa ctgtgttctt cacaccagcc tgttttaatt gaaccaatgc
      721 gtctacggca tcttctttca aggtatctgc aataacaaga taaccactgt aactttgatc
      781 gatcgctaga taaagcaatg taccgatttc ttgacttaaa ggaaaagaga tttcaaatct
      841 ttccatcaat ttgtgattac ctgctaatac ttgtttgcca tcaacttcag ctaagatccc
      901 ttcacctgca aattcctgga tattagttgt tggtaaaagc ggacctgcgt agccttctaa
      961 aacagattga gcaatcggat gtgtagaaaa ctgttctgca ctcgcaatat attgcatgaa
     1021 tttctttgga tcgatttttg tatcgatcgt ctggatcgaa aagtcacctt tcgttaatgt
     1081 gccggtttta tcgaatacca ctgtctcggt ttgcgcaagc aactccaagt aatttgaacc
     1141 ttttactaat acaccgattt tacttgcgcc tccgataccg ccaaagaaac taagtggcac
     1201 ggaaatcact aatgcacatg gacatgagat cactaaaaat gtcaatgctc gatagatcca
     1261 ttcagaaaac gccgctccag aaacaacaag tggaggtaaa acagctaata aaacggccaa
     1321 tcctacaacg actggtgtgt aatagcgcgc gaatttagta atgaaatttt cagctggtgc
     1381 ttttttactg ctggcatttt ctactaattc taataatttg ctgatagtag aattttcata
     1441 agatttttct acttgaacta aaaatggttt atttttattg ataaatccgc ttaatactgt
     1501 ttcaccaaca tatacagatt tagggactga ttctcctgta agtgctgacg tatcgactaa
     1561 tgattctcct tcgatgatcg ttccatctaa cggtacacgt tcaccaggct tcacaagtac
     1621 aaactgaccg atctgcacgt cttcagggtt cacggtcatc aatccttccg tcgtctgcac
     1681 gtgagctgtt tctggacgga tcgccatcaa ttcgcgaatc gattttcttg aatgactgac
     1741 tgccaatcct tggaaatact cgccgatttg ataaaatagc atcactgcta cggcttctgg
     1801 atattcaccg atcaacatag cgccaatcgt agcaacagac ataagaaaat tctcatcaaa
     1861 gatttttcct ttgacgatat tcatcactgc cttcttcact acatcatatc ctatcagcag
     1921 ataaatcaat aaatacccta tcaagcgaat tggttcatta aaatcaaata atagcaataa
     1981 agcgatacct gcaagggaaa ataaaatccg gaacagcgta tttctctctt tttcatttcc
     2041 atgttcagat actttttcct tatctatttc tgttactttg acatctggtt ctaattcctt
     2101 cactactttt tttgtttctt gttcaatcgt agccaaatga tcttcctctg cgtcaatcgt
     2161 tagttttcca gaagtgaagt ttaccgttgc ttccttgact ccattgatct tctgaactcc
     2221 tttttcgatt ttcattgcac agttcgcaca atctaaacct tctaatctgt aacttttcat
     2281 tttaatgacc tcctcgcctc aacatatgaa catctcttca taactaatta taaatgaatg
     2341 gttattcata tgtcaaggta tccgaaagaa aaaatgaaaa acatctgcat cggaaaattt
//
LOCUS       DAPCMT010000221         2304 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPCMT010000221 DAPCMT010000000
VERSION     DAPCMT010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.230"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            88..840
                     /locus_tag="RD743_002788"
     CDS             88..840
                     /locus_tag="RD743_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDU0564552.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(980..1903)
                     /locus_tag="RD743_002789"
     CDS             complement(980..1903)
                     /locus_tag="RD743_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0564553.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(1918..2190)
                     /locus_tag="RD743_002790"
     CDS             complement(1918..2190)
                     /locus_tag="RD743_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564554.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT          759 a          458 c          393 g          694 t
ORIGIN      
        1 cataatttca tgagaaaaaa atacaatttt ttcttgacca tctcgttacg taaaggatta
       61 tgcttacatt tgtcaggagg aaaaataatg gagtacacaa taaagaaaat gtcagagata
      121 tcaggagtca gtccgagaac cttgcgcttt tatgatgaga ttggactgct aaaaccagct
      181 aggatcaatt cttcaggtta tcggatttat ggcaaaaaag aagtcgatcg tctgcaacac
      241 atcttatttt atcgaacaat ggcattcaag ttggatgata tccaagaggt cttagacaat
      301 ccatcttttg accaccagaa agctttaatc aagcatcagc aaatgttgct ggaaaaacgg
      361 gcacaaatcg ataccctctt gacaactgtc caacagacac ttgatatgta cgaaggagga
      421 agaaaaatga gtgacaaaga aaaatttgaa gggtttaaac aacaaaagtt gaaagaaaat
      481 gaagaatcct atggacaaga gattcgacaa aaatacggaa aaaaaaaggt tacagcatcc
      541 aatgaaaaat ggatgaatat ggataaagaa acgtatgaag aaatgcaaac tgttgaaaag
      601 cagatgttat ttgatctcac tctttactta aaagaaccag aagatgaaag cttagcagac
      661 cgtatcttcc aagctcataa gaagtggtta tcttacagct ggcctgaata tcagccaaaa
      721 gcgcataaag gactaggaat gatgtacgaa gccgatgaac ggttcactgc atattatgac
      781 gagcgaagtg gtcatggtgc agcaaaagct ttaaatgaaa ttatccaaaa aaaggcgtaa
      841 tcttttacat gatccaacaa aaaagtacac gtaaaatgac ctaataacaa aacagcagac
      901 acccaagtta gatttttatg ctaacttttg gtgtctgctc cattctttca caagtttttt
      961 catcctactt taaatatatt catcgttgaa taaacgtata aggtattttc actttatcct
     1021 ttttttctgt aagaaacaat tgaaatgcca tttctccgca agcttccaga tggtggtcga
     1081 tggttgggaa accaagaagc tcgctgatca gcaaattatc gcgtccaatc accactggtg
     1141 tcttatcttg cagatagctt tgcaagatgc ctgctgcaac catatccgaa tttgtaaaga
     1201 caccatcaac tccaagctct ttaaaaaaga agcctgcttg ttttcctttc tctgcatcta
     1261 tacaatccca aaaaacaagt gattcatcaa aagatggaaa aagttgatga catagctgca
     1321 aagtgatttt tgagttataa cttagcctgc cacttcgccc caacgtgatg cccaattgct
     1381 ttataccttg ctgcttcaaa taagtaagac cttctgtaat tgattgttct cgatcaataa
     1441 agacacagct tgcctgtttt cccttgattt cttcacaaaa aactagaggt ccatattttt
     1501 gatatgccaa taaatcatct agtgtattgg cacgagacgt aatgatcaat ccatcgaaag
     1561 ctttagctgc aaattcttca agataccttc gctctttttc tttctgatag cccgtcggta
     1621 aaagtgtcac tttgtaatca taatcaaacg ctgcttcaat gatcccactg atcaactgat
     1681 caaaatacgg atgattggta taaggcagaa tcactccaat gcttttcgtt tttccataac
     1741 ttaactgtct ggcatttgca ttagggatat aatcgacttc tttcatgacc gcaagtattt
     1801 ctttcctttt ttcctcatct acatatggat gtccattgat tacacgtgaa atagtagaaa
     1861 ctgaataacc tgtgagcttt gcgatatctc gaatgtttgc cactgctgct tcgccaccta
     1921 tacaaaaaga ttttcttgta aatagtctac cacagctgga ccgcattcaa aaaactgata
     1981 catttttcct tcatgcgaaa aactgatccg ctgcccgccc caatatatat cttttgtcaa
     2041 attttcgatt tcattcattg gtatttcttt tactatattc ctattcaacc cttgaaaact
     2101 gtacaacgtt aatacggatc ccttgaactc gacaaacgca taaaaataat tgttcgttgt
     2161 gccatctgtt atctctacat aagtattcat tgcaagccct ccttttgata ctttgagtat
     2221 agtgtatgaa acgcgtctca tagcaaggag tttgttttgc caaagaaaag ttttattcct
     2281 ctttcattat taccaaagtc tctt
//
LOCUS       DAPCMT010000222         2264 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPCMT010000222 DAPCMT010000000
VERSION     DAPCMT010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.231"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            100..1713
                     /locus_tag="RD743_002791"
     CDS             100..1713
                     /locus_tag="RD743_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDU0564555.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          708 a          402 c          445 g          709 t
ORIGIN      
        1 ttttctgtct attcaaaata aacaataatt ttccggcaca attatttgct gttttttacc
       61 ataacatagt acgatcaacg tagaaaagga gatgttttta tgaaagttgt tgtcattggc
      121 ggtgtagctg gcggtccttc atttgccact cgtttccgtc gattgaatga agcgcacgaa
      181 atcattatct atgaacgcgg agaaaatatt tcttatgcaa gttgtgcttt gccttattat
      241 ttaggaggtg tgatcactga ccgtgactcg ctgatcgaac gtacaccaga aatattgaaa
      301 acaaaaaaca acatcgatgt ctttactaaa cacgaagtaa cagcaatcga tccttctact
      361 aagcgattaa cagttaaaga cctatccaca aatgaagaaa taaaaacaga ttacgataag
      421 ttgatcatct cttctggtgc tagaccagat tatccggata ttcccggagt ttttgaagca
      481 gaaaacggct ttgtcctccg tagtgtgacg gatgcggatc gaatcaaatc gttccttgaa
      541 gaaaaaaatc cacaacatgt cgtcattctt ggtgcaggtg ttatgggtct ggaattagct
      601 gagaatttca agcatcgcgg cttagacgtg actttaatcg atcaattgcc acaagtcgct
      661 ttcccttatg atccagaaat tgctaattta gtttatgaca aattgctgaa agaaggatta
      721 gccgttcatt tagaaacaag agttactgag atccgtgata aaggtcgaga aatagtatta
      781 tcagatggct ccgtcctttc tgctgatatg ctaatttttg ctgttggtgt ttctccgaag
      841 aatgaagtgg tgaaagcaac cggcatacaa ttatcagata caggtcagat cattgttgat
      901 gaccagttac aaaccaatct gccggacatc tatgcgattg gcgatattat cgaaacgact
      961 agtgtagtga ctggtcagcc gatccaaagt atgctttcca gtgcggccaa tcgtcaagga
     1021 catatgctgg cagatatttt aaatggtacg cctatgcgct atcgcggata tattggtgca
     1081 ggtgtcgcaa aaatctttga tcatacagca agttatgcgg gaatgacaga acatgaatta
     1141 aaagcatcag gcataacaaa ttataaaact gtttttatca ctccctttga ccatgcctat
     1201 ttctatccag aagctacaag attaaatcta aagctgattt ttgatgcaga tagcggtcgt
     1261 attttaggtg gacaagcatt tggagaaaaa ggtgtcgata aacggatggg agaactttct
     1321 gtagcaatca ccggaaactt gacagtcttt gatttgcctg atttggagtt gccttactct
     1381 ccaccgtatt ctactacccg tgacccgttg aatatagctg gttatgtcgc aatcaatcaa
     1441 atgacgaata tcgtagaaac gatcaaagca agtgatatac ccgaaaacga tttgaaagaa
     1501 gcgttctttt tagatatacg tgaacctaat aaagcacctt ctggcagtat ctcggcaaca
     1561 aaaaatattc caatgaatga gttacgagat cgaatcaatg aaatcccaaa agataaaaaa
     1621 gtttacatta ctttcagaag aggattgaat acttatactt ctgcccgaat cttggcaggt
     1681 ttgggtatca aagcgatttt gattgaagaa taaaaaaacg accgcggtgg gtagccgcgg
     1741 tctaggagtt atttttactt gaggagtaaa aatgaaaaag tatatagggt tgttggttgg
     1801 tatgtatata gaatagaaga taaatgtgaa gaaattgtgc attatttctt cacatttatt
     1861 aggttatagt ttaagaaagt ttaaacattg cgcaatttaa ttattttagc tgtttctttt
     1921 cataaaaaaa catgttgtga ctctgagaca aatcatagtc catagtgatg tctctagagt
     1981 aactacgcct aatattacga ggcatcatat taatctatca gcactagtac ctaaactagc
     2041 atccctttcg aatatttttt cttatataat aaagtttgct ttcactcctc taatctcacc
     2101 aaatcgactg gcttgcgttg tgtgatacta ggaactgatt ggtttagaac gacttttcta
     2161 ttgtaatcat atttttcgcc atcctcgttg tcttctaatc cttcaatgaa tgatccctaa
     2221 tgtgtgtatt aaataattta atttttttgt tcctatttat ttaa
//
LOCUS       DAPCMT010000223         2232 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPCMT010000223 DAPCMT010000000
VERSION     DAPCMT010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.232"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..544
                     /locus_tag="RD743_002792"
     CDS             <1..544
                     /locus_tag="RD743_002792"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDU0564556.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="RD743_002793"
     CDS             complement(900..1472)
                     /locus_tag="RD743_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0564557.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="RD743_002794"
     CDS             complement(1488..2093)
                     /locus_tag="RD743_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDU0564558.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ct
//
LOCUS       DAPCMT010000224         2221 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPCMT010000224 DAPCMT010000000
VERSION     DAPCMT010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.233"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..322
                     /locus_tag="RD743_002795"
     CDS             <1..322
                     /locus_tag="RD743_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0564559.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            736..1554
                     /locus_tag="RD743_002796"
     CDS             736..1554
                     /locus_tag="RD743_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDU0564560.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1627..1986)
                     /locus_tag="RD743_002797"
     CDS             complement(1627..1986)
                     /locus_tag="RD743_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDU0564561.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          749 a          319 c          414 g          739 t
ORIGIN      
        1 ttataaagca atcattcctg agaacttaga tgaggttaaa ctttatttat gggaagataa
       61 cggagaaatt gtcggtttta gtggtgtaag tgcagaagaa ttagttatgc tatttcttga
      121 tccagatttt attggtaagc gatatggtag tcaaatatta attgagctga ttgaaactga
      181 agatataaaa agaattgatg ttaatagtca aaataaatat gcaaaacatt tttatttaag
      241 ccatggattt gaaattgaat ctgaagaaga cttagatggt tttggtaaac cataccctat
      301 aacgcattta gtaaaaaaat aattcatgaa tggtttatta cctgattgaa catgaataaa
      361 agtaatattt ttattttgat ggcctatttc taggttatga attatccaga tcaaatttgt
      421 gtattacatt cagtaataag gtgcttatct taggatcaac ttatgatact agctgaccaa
      481 ggatgtacca gttatagaaa atcagttaaa gtaaaaatga taaaaatctt atgtcaacac
      541 tgatttaggc tgacctgtgg gttaggagaa agacaggtcg gtagctttta atacaaaaaa
      601 tggcttgtat gtatctagat agttaactga aattagaaat gttcttattt ttaacggagt
      661 aagaaaaata ggctgcttta ggtctgaata ctttttcaat ataatgaaga tatcaaaggc
      721 gacaggagtg tttttatgaa taagaatcct cataatctaa agttggtagc agtagatatg
      781 gatggaacat ttgctcgtac tgattacaca tacgatatcc caaggttcaa ggctgtttta
      841 tcgcgaatga aagctgttga ttgtcaattt gttgttgcaa gtggaaatca atactaccaa
      901 ttgagatcgt tatttccaaa ttattatgat gaattaagtt ttgttgctga aaatggtgct
      961 tttgttaaag ataaaacaga ggtactattt tcagccgata tgcctagaaa agcgatcgag
     1021 gtggtccttg atttatgtaa agactatcct gaagtaaaaa atgtgatgtg tggtctgaat
     1081 agtgcctact gtgaaagagg aaaggtgagt caagcatttt ttgatctaac caatatctat
     1141 taccatagat tagaatgggt agctgatttt gcagaagttc aagataaaat tttaaaattt
     1201 gctccgacta tgcctgttga aaaaaccgaa ttttacgttg aattgttccg tgaaaaatta
     1261 gccgggctat tagtacctac cagtagtggt catggttctg tagacttgat tctttctgag
     1321 tgccataaaa ctttcggctt gaaaatgtta gttgagcgct tggggattaa tcctgaccag
     1381 tgcgttgctt ttggagatgg gggcaatgac attgagatgt tggagtattg tggtctgagt
     1441 tatgaaatgg ataatgcaac ggaagctgtt aagcaagttg ctaagcatca atgtccttca
     1501 aatgacgaag acggtgtttt agtgacgctc gatcgattgt tccctaacac gtaaacacta
     1561 ggtaattcat caaaaaacct cgatataaga aatattcgag gttttaaaac tatatttaga
     1621 acagatttac gaagcgtctg atgagtcaaa atagacttgg acattgctat ctaaaatacc
     1681 actttcattc tttaaacgaa ttgtttgatt tgatggagtt acctctaata ctaaatgccc
     1741 attaataaag gtagtatcgt attcagatgt tttaactgtt gcttcttcgc aaggtattgt
     1801 taaatcatat gctggaatat aatgttgtgt ggaaactaat aaaaaatgat caccaatcat
     1861 acagcatccc tctgctgaag aaaattgatt agagccatca tttaaagtta acaagaatcc
     1921 atcctttaaa cgaggtactt gcttaaatac ttctggttgt acttctaaaa ataaattatc
     1981 gctcattttc acgaccacca tcgacttcca cagttgaaat tgacatttta tttaacacac
     2041 ctattccccc ttaactataa ctcaattcta tataaaaaat attctattct caaataattg
     2101 aacttttcaa atcgccaatt tacttcggct ttatcagagc tcattcatat caagagcgat
     2161 taacgctgta aaaattaaac cgaagatctg ataaattttc tctattttct acaatattta
     2221 a
//
LOCUS       DAPCMT010000225         2196 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPCMT010000225 DAPCMT010000000
VERSION     DAPCMT010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.234"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            63..452
                     /locus_tag="RD743_002798"
     CDS             63..452
                     /locus_tag="RD743_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HDU0564562.1"
                     /translation="MSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIA
                     VENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTI
                     AFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            580..1263
                     /locus_tag="RD743_002799"
     CDS             580..1263
                     /locus_tag="RD743_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDU0564563.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1572..1645
                     /locus_tag="RD743_002800"
     tRNA            1572..1645
                     /locus_tag="RD743_002800"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1606..1608,aa:Arg,seq:tct)
     gene            1804..2163
                     /locus_tag="RD743_002801"
     CDS             1804..2163
                     /locus_tag="RD743_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDU0564564.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          732 a          343 c          450 g          671 t
ORIGIN      
        1 ttctcgtcgt cttggttccc cgaatttttg gtttgactcc ttatattatt gaggacaaag
       61 aaatgagtcc atattatgca caaggaaatc tgatatatgt acgaccgaaa aatacaaatg
      121 agattttagt cggagatatt attacatatt atgaaaattc aggaaaaaaa atctcgacgc
      181 gtcgcgtaat agccgttgaa aacaaccacg aagattttta tacaaaagct gatacaaaga
      241 cttatattga gcctggagta gtaaaaaaga gaaatattat cggtagtccc atctttcaga
      301 ttccatatat tggactagtc ctttcaaaaa cggcatttgc atggattcag gggttattct
      361 ttacgattgc tttttgccta acaggaatca cagcatggaa tacctttgta gagttgaaaa
      421 agaagcggag acgaacacag aaatttttgt aagaatgtta aaaacataaa tgtattccag
      481 agatccaacg actcttctga acatgaaaca cgagaaaaca ctcatgcagc ttgtagattt
      541 agtcagttgt tcgtataatg tgtgaagagg aggagtagta tgcgcaatga aatgatgcat
      601 cgtcctgtag tcaaagaaga actattggat tttatgcgta cccagcaaaa aaaattaccg
      661 ggtgagctag gcaaactgga agaagaagct catgtagcag aagtaccagt tattccccat
      721 gaaacagttg tatttttgaa atttttactt ggacagcttc agcctgaacg aatcttggag
      781 atcggagcag ctatcggttt ttcatcaagt gtcatggcca cgacaatcgg agaaaacgct
      841 catgttacga caattgatcg tttcgatgtc atgatcgaga aagctaaaaa aaactatgaa
      901 cgtctaggac tgacagataa agtgactttg cttgaaggtc aagcagcaga tatcttgccg
      961 gagttgtctg gtccgtatga ctttattttt atggatagtg caaaatcaaa atatattgaa
     1021 tttttacctg aatgcttacg tttgttaaga aaaggtggcg ttttgatggt agatgacatc
     1081 ttccaaggag gaacgatctt gctcccagat gaagagattc cccgtggcaa acgagctatc
     1141 catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc atcccgattt gacatcgaca
     1201 atcttgccat taggtgatgg tgtgatctta atgacaaagg aatcagaaac aatcgagttg
     1261 tgatacatga ttatggcagc ctgtagataa tcgattgaat cgagggctca attcgaactt
     1321 gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata caggaaatct aaataaacag
     1381 gagtagcatg aagtcaatgt agctcctgtt ttcaattgat aaccttagaa cagctgaagg
     1441 agaatgtctc gttttaaaag attgatttag tagttgggcc aaaataattc attatttgct
     1501 aattaaataa ctatttcgta ttgacagaag caatttaatt atctataatt attgatgttc
     1561 cgctgattct tgtcatagta gctcagctgg atagagcatc cgccttctaa gcggacggtc
     1621 gggggttcga atccctcctg tgacgctaaa aaaccgttag aaactgaggt ttctaacggt
     1681 ttttctttgt tttttgtgcc aatattgttt gttttcaaag gcaaataaaa taaaaaaaca
     1741 tccttatcga gtacaattat aaggagcaaa aaaataaaac tcatcagaaa agaggaagaa
     1801 gatatgacga tactatctag aattttagtg actctagttg caattgaatt tttttatatc
     1861 atgtatatcg aaacggtgag aactgattct gacacgacaa gtagggtttt taaaatgagt
     1921 aaagaagagt taagcagaaa atctgttcaa acattgttta agaaccaagg agtgtacaat
     1981 ggactattag gagtgggact actttatgga gcttatcttt cttccgcatc taaagaaatc
     2041 acaagtatgc tattaatatc tatttttttt gttgcattat atggcagttt gactagcgat
     2101 cgttggattt tactcaaaca aggtgggcta gctctgttgg cattgctttc tattttctta
     2161 taaatataga aaaaataaga atttatctaa caggaa
//
LOCUS       DAPCMT010000226         2140 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPCMT010000226 DAPCMT010000000
VERSION     DAPCMT010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.236"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..351
                     /locus_tag="RD743_002802"
     CDS             <1..351
                     /locus_tag="RD743_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564565.1"
                     /translation="QTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTI
                     SSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNF
                     RARIYLQQGLIFEN"
     gene            406..1971
                     /gene="guaA"
                     /locus_tag="RD743_002803"
     CDS             406..1971
                     /gene="guaA"
                     /locus_tag="RD743_002803"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDU0564566.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          660 a          445 c          446 g          589 t
ORIGIN      
        1 caaacagaaa tcgttgatga gctgttaagc tacaacgaag aattgcgaac tgcgtatcat
       61 ttttgtcaat tattacgcta ttatttcgtt aaacgaggta cggaaggctt tcaagaaacc
      121 cttaaaacaa tttcatctac gttacctcag tcgtttaaaa agaaattcac gatcttccat
      181 cgctaccaat cgggtatttc aaatgccttt aaggtgtccc actcaaacgg cgtcactgag
      241 gggctgaata acaagatcaa gctgattaaa cgaattgcgt ttggttatcg caacttttat
      301 aactttcggg cacggatcta tttacaacaa ggtctgatct ttgaaaacta aaaaaatatg
      361 cggcgcacag aacgcgccgc atatcggact aagttttatt caaatttgtc ttcaccaaca
      421 cgatttgacg aagagccaaa aattatcgta cttgattacg gtagtcagta taaccaactg
      481 atcactcgcc gtattcgtga gttcggtgtt ttctcagagt tgatgagcca ccgcattacc
      541 gcagaagaag taaaggcaat gaaccctaaa ggaatcatcc tttctggcgg accaaacagt
      601 gtttatgatg agaaggcctt tggtattgat gaaaagattt ttgatttggg gatcccagtt
      661 ttaggaatct gttacggtat gcagttgatt acttataaat taggcggaaa agttaaacca
      721 gccaaaaacc gtgaatacgg aaaagcagaa ttggaaatca cttctgcaga agcggccctt
      781 ttccaaaaca ctcctgaaaa acaaaccgtt tggatgagtc atggtgacct agttacccaa
      841 gcaccgactg atttcgatgt cgttgctacc agcaaagatt gccccatcgc atcgatccaa
      901 aatgcagaac gcaaaatgta cggaatccaa ttccatgcag aagttcgcca ttccgaatat
      961 ggaaatgact tgttgcgcca ttttacacta gatgtctgcc aatgtactgg tgactggaca
     1021 atggataact tcatcgacat ggagatcgca aaaattcgtg aacaagtcgg tgacaaaaaa
     1081 gttttattag gcctttcagg cggagtagat tcctctgttg ttggtgttct tttacaaaaa
     1141 gcgatcggcg atcaattaac atgtatcttc gttgatcacg tattgttgcg taaaggtgaa
     1201 gcagaacaag ttatggaaag tcttggcgga aaatttggct tgaacattat aaaagtcgat
     1261 gccaaagaac gtttcttatc aaaacttgct ggcgtgtctg atccagaaga aaaacgtaaa
     1321 atcattggaa atgaatttgt atatgtcttc gatgatgaag caaccaaact tgccggtgaa
     1381 gaaggcgttt ctttccttgc acaaggaacc ctttataccg atgtgatcga atcaggaact
     1441 gaaactgccc aaaccatcaa atcacatcac aatgtaggtg gattacctga agacatgcaa
     1501 ttcaagctga tcgagccatt gaacacattg ttcaaagacg aagtacgtga attaggtacc
     1561 caattaggca tgcctgacag catcgtttgg cgccaaccat tccctggacc aggcttaggt
     1621 attcgtgtcc ttggtgaatt gacagaagaa aaattagaaa tcgtccgtga atcagacgct
     1681 atcttgcgcg aagaaatcgc caatgccggt ttagatcgcg acatctggca atacttcacc
     1741 gtcctaccag gcattcgctc tgttggggtt atgggagatg gccgtacgta tgactacact
     1801 gtaggtatcc gtgctgtcac ttctattgac ggcatgaccg ctgactttgc ccgtatccct
     1861 tgggaagtct tgcaaaaaat cagtgtccgt atcgtaaatg aagtcgcaca cgttaaccgt
     1921 atcgtgtatg atattaccag caaaccacct gcaacagtgg agtgggaata aagagccaac
     1981 ataatatgga tgaggaatac ctataaatca acattattca attactaaag ttcctgattt
     2041 tcgtaaaaaa taagatggtt tcctaccatt ttccctacca aagcccttgt tagattaact
     2101 actaacaagg gctttgcttt tattcactac ctttattttc
//
LOCUS       DAPCMT010000227         2115 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.237, whole genome shotgun sequence.
ACCESSION   DAPCMT010000227 DAPCMT010000000
VERSION     DAPCMT010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.237"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(540..905)
                     /locus_tag="RD743_002804"
     CDS             complement(540..905)
                     /locus_tag="RD743_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564567.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(981..1226)
                     /locus_tag="RD743_002805"
     CDS             complement(981..1226)
                     /locus_tag="RD743_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0564568.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1296..1550)
                     /locus_tag="RD743_002806"
     CDS             complement(1296..1550)
                     /locus_tag="RD743_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564569.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1714..2037)
                     /locus_tag="RD743_002807"
     CDS             complement(1714..2037)
                     /locus_tag="RD743_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564570.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          793 a          361 c          372 g          589 t
ORIGIN      
        1 aagaaagcgt tattatttta gtctattttc agggggggaa ccttatgata aaaaaactat
       61 ttttactatc aatattaacg ctagcaatgg caggttgttc tgactctggc ttagcaactg
      121 aactgatcac aaaatcaact gaagccacga cagaggagtt aaaatgtgga aacgatcaca
      181 gtgactacag aggcttaaat cgacgatgca tgggaatatt aggcagaagc aattgtaagc
      241 atcgatgtag taatcgaaga aggtgtagaa gaagcaacag aaaaatcaaa agaaagatca
      301 aaagaagcaa tctgtgctgt gcatcgtata cagacgtcga agatgcggta cttttaaacc
      361 acgtcaggag atgttcattt atgtctaggg aagttggcga tgaaccaaaa ctagagtacg
      421 cttttagttt tgcacgtgag gatgaagata accctaaaac ttacgacatt ttagcttgat
      481 ctaagatggt taaagctttt taatgaagta agaggagata gaaggctttt ctagttatat
      541 taggtaagat aaattccttt ttgctccaaa tttgctaaac attctgctag ataaacttca
      601 tcgtgttccg gataaatcgg cacgttgcgc tctacagtag gtaaatcaaa aaatgcttcg
      661 catgatttgc cacgatacat aggatttttc cactctggtg ctgggctgta accacgtttt
      721 tccatttcat ccatgacaag ctgatgatat tggaataatc tataaggaga atagtgaaaa
      781 acataatcca cagtagcatg tttctttccc cagccatttc ctcttaatgc acagcattcc
      841 cgatgttgtc ctaaaagctg ttgacgagga agctttgaaa tcaatttttc atgccataat
      901 cgcatagaaa tgtctccttt atatatagat tgttaacctt agacgttaaa tgaaaaaacc
      961 agcaccaagg ctggaaaaaa tcacttcagt ttgcttgctg tttctgagcg accaattagg
     1021 taatcaacgg aaatatcgta aaagtcagcg atatagatta attcaatgat gcttggaatt
     1081 tcttctcctt cttcgtactt acgatatttg aataaaggaa tacttaacgc gctagaaaca
     1141 gctacttgag ataaagatcg gccagttctt aagaaattca gacgacaacg taaagtatcc
     1201 agtaaaatag cttcttcgat actcatttaa tcacctttca ttatagtaga atagttataa
     1261 tgactataga aagctacaca tgcaatcaat agtactcaat gttttttatt taaacgtatc
     1321 aaataaagaa tgacgatcaa taaagacaaa accagtcctg ataattcttc tataaaaaca
     1381 ctatcgggtt caaagtagtt gtaattaaaa actttccaca taaagtagat agccggaaga
     1441 gtcaaaatag ctaaataaaa ccgttctttg ttccaattca taagataaac aatacttgcg
     1501 accagtgcga ccgcaggcac ccaaaacaaa actctcacca acgtaatcat gtaacggact
     1561 cctcaacgat tcatataata aaagataaca tgttattgca tacatcacaa atgaaatgct
     1621 aggaaaataa aagaagaata gatcaatgaa aaatgaaaaa gcagaacagt tttctgctga
     1681 taagcaggaa ctgttccgct gaatgtgtct tatctatcga gaaaaaatca atggattgat
     1741 atctaagtaa ttcaatacat ataaaaagag gacgatcgca ttcactaaaa aggaaagaat
     1801 gatgatccca tagcgaatgg aaacatattt aacaaatact tttgtttgaa tacgggcata
     1861 tctttttcct ttatagctga gcatcatgac aagaagcaaa agggtaatca caccttgaaa
     1921 aatcattcca ataaacagaa ttcctgaggt ttgataattt aattggagaa aataagctgc
     1981 tgccgcaact aaatcgaata aaatcgcaat aaatggcaaa ataatcactc ctaacattga
     2041 gaataaataa aagaataaca ttttttctaa aataagcaaa taaaaattaa agtaacaaaa
     2101 tagttatgac acaag
//
LOCUS       DAPCMT010000228         2065 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPCMT010000228 DAPCMT010000000
VERSION     DAPCMT010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.238"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            148..>2065
                     /locus_tag="RD743_002808"
     CDS             148..>2065
                     /locus_tag="RD743_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDU0564571.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEG"
BASE COUNT          804 a          278 c          359 g          624 t
ORIGIN      
        1 aagcctgtga tataaatatt gcaggctttt ttattgattt gaaataatca agaatattag
       61 aaacttctac attaatcaag aactttcttc tgtatgaaaa tagagataat aattgtacac
      121 ttaagctgta ttcaggaggt gaagataatg ggcatcgaat ttagcgaacg gcaaatgaaa
      181 atactatact ttttgattga aaatgagcac acaacttaca aagaattatc agaacaatta
      241 tatgtatcta cgaaaacaat tagcaaagat attttaattt tgaaagaaat aatccaaaaa
      301 atgccagatg tacaaatgtt ggttcaacct aaaaaaggcg tttcgcttat tggaaaaaaa
      361 gaaacacttc gaaatttatt atccagtcat aaaatcaata tcgcagactc tccggaggac
      421 cgtctaactt atgtgtttgc taaattattg aatactaatg attctctaaa gattcaaaat
      481 cttgctgacg aattatacgt gagcaggtcg acggtagaaa gcacaatgaa agatgttaga
      541 aaaagactaa acaagcatgg gatgtcctta gtttcagatc gaaatggcgt gcgcatcaaa
      601 gctgatgaaa aaagtaaaag aaggttgatg tcagaagttg tgaactatta ttggggtggc
      661 tttacagcat ctaacagtaa aacagatctc ttagacttga atatcaattt cattggtgaa
      721 gcgaataatt tcttaaataa ggacatattg aaaaaagtag ccgaattgct taatcgatac
      781 atgaataaga cacaattgaa atttacagat tatgaatttc aaactttagc tatccacctt
      841 gctattgcga tagaaaggat tgaaacaagc aatttcttat cacgtagtaa taattctgaa
      901 gaagaaatct caaagaatac acttatttta atcaaaatga ttgaaaaagc attttcaatt
      961 attgtgccaa tagatgaaca agcttatgtt gacattcaca ttacttcgat tgaaaaaaat
     1021 gctatgaatc acgacttaga tcaaacgaag ctttcctatt tagatatggg tgttgagcta
     1081 caagagttag taagaattaa attaaatgaa ttttatcctg atgaagaatt gatcaataat
     1141 ctagtattac atctcaatgc agcagttaaa agactcaaat tgggagtaaa tatctataac
     1201 ccttatacgg acaaaatcaa atattctttt aaaaggtcat ttatgataag tgtagattta
     1261 ttagaggaaa tcgaagagag attctgtata cattttaatg aagatgaaat cgcttacgta
     1321 acactccata ttcaatcact tttggatcgt tacaaacctg acaaaacgaa agtgatatta
     1381 gtttgtagta gcggatatgg aacatcaaaa ttattagagc aaagaataaa taacggtttt
     1441 gcagatatgg ttgagataga agatgtacta agcatcaatg aactacaaga tagaaaagtc
     1501 actgatgaac ttgttataag cacgattccg attgaaaata caaattttcc tgttattgtt
     1561 gttagtcctt tgatgttagg ggcagatgtc agaaaagtaa aacagtatct acagttaaat
     1621 gaacaaacag aagatgcttt tctaaattta ttagataaac gattttgctt ttttacccac
     1681 caaataaagg ataatcaagt gaacgtttta aatataatta ccaaaaaatt ggtaaaagaa
     1741 aattacgcga aagaagggat actagaggga gcaatcaaaa gagaaaaact atcttcaaca
     1801 gcgatgaatg attttgctat gccgcatatc gaagtagatt ttataaaaaa gccagtaatc
     1861 tgtgtctacg ttaatccaga aggaattgat tgggatggga cacttgttca ttctgttttc
     1921 ttttttgcac ttaatgaaaa agtaaaacct gaattgaata aaatttatga aacattcaat
     1981 gaaatcgctt cgaatcatga gttattagca aatttaaaaa cgagttcgtc gtttgaaaaa
     2041 ttagtttata tattgaaaga aggct
//
LOCUS       DAPCMT010000229         2058 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPCMT010000229 DAPCMT010000000
VERSION     DAPCMT010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.239"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..337)
                     /locus_tag="RD743_002809"
     CDS             complement(<1..337)
                     /locus_tag="RD743_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDU0564572.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="RD743_002810"
     CDS             complement(404..559)
                     /locus_tag="RD743_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDU0564573.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="RD743_002811"
     CDS             1176..1397
                     /locus_tag="RD743_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564574.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="RD743_002812"
     CDS             1398..1655
                     /locus_tag="RD743_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564575.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="RD743_002813"
     CDS             complement(1791..>2058)
                     /locus_tag="RD743_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDU0564576.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          679 a          461 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcaa cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DAPCMT010000230         2018 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPCMT010000230 DAPCMT010000000
VERSION     DAPCMT010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.240"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            3..170
                     /locus_tag="RD743_002814"
     CDS             3..170
                     /locus_tag="RD743_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDU0564577.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(230..583)
                     /locus_tag="RD743_002815"
     CDS             complement(230..583)
                     /locus_tag="RD743_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564578.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1088..1507
                     /locus_tag="RD743_002816"
     CDS             1088..1507
                     /locus_tag="RD743_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDU0564579.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSCDCIFFKVLNYTIQVSNEKLAVALYKLSSKERDVILLRYFQ
                     SMSDQEIAELYHVSRSAIYRRRSNGLKKLKIVLKERN"
     gene            1549..1752
                     /locus_tag="RD743_002817"
     CDS             1549..1752
                     /locus_tag="RD743_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564580.1"
                     /translation="MCQASAGSETAIREILKFYDAYICKLCLRPFYHSESGKITMQVD
                     EELKGQIHTEMMKAILKFEIRVK"
BASE COUNT          692 a          314 c          358 g          654 t
ORIGIN      
        1 tattgtgtcc tgtatgtgga aataaaacac gattaaagat aagggaagat actgaattaa
       61 aaaaattccc cctctattgt ccgaaatgca gacaagaaaa tttaattgaa ataaagcagt
      121 tcaaagtaac tgtgattaca gagccagacg caaagacgca gagccgataa aatgagatta
      181 atacaatctc attttatcgg ctctttccgt tatgtatgga ttcttttaat tagtcttcga
      241 tgtttcttgc ttcgttgata ccgctggcta aagattccat taaggatagt tctttgtctg
      301 taaagctatc catgtatttc tctatctgta atcgtcgggt gctttttacc aagttattag
      361 caggtaagaa aaattcatca acggaaacat gaagtaacga tacaaggtca taaagaactt
      421 gtatgctggg gtgttgccct ttattttcaa tattagttaa gtaccgtggg tcaatttcaa
      481 tcaatgctcc cacttgttca cgagttaaac ctcgtttcaa tcgagcttct ttaatggcta
      541 aaccaaaggc tctaaaatca tatttatctt cttttttacg catagtagac cacctctata
      601 cattttattg ttcctactga attaaaaaca ggtatagaaa aacgtgttat atggtttata
      661 ggtttatatt taataaaaag cactactaaa cgccaataaa aaaaaccgtt atatggtagt
      721 gctatttacg ctgttaaaat attgtatatt acttccaaat ggcggtttgt tggaggtcaa
      781 cgtcgccatg aagtacatca tatacaataa atttccttac attgggttct tgtcaaaaaa
      841 agtcgtctat ctgcaataga taagtacgtc caccaatgtg gttttataaa tcatatagat
      901 agaataacag aagcatgtaa acagagaaat aaatctgttt atatgctttt ttggctattc
      961 agaacttttt tacaaagttt atttatcagt aatgcaacaa atcccccttt cacattggga
     1021 ctaagagtga aaggagataa acgagcaagg ctcacttcct ttcctagaca gaaagggggt
     1081 gagaaacatg aaaccatctt cttttcagac cacaatagaa aatcagtttg actatatctg
     1141 taaacgtgct atggaagacg agcgaaagaa ttatatgctt tatctttcaa ggattgcaaa
     1201 gcgtgaggtg tccttttcgg atgttggcga ttatcttgtt agccagtttg cgacaacaga
     1261 taactattcc tgtgattgca ttttttttaa agtcttgaat tatacaatcc aagtatcaaa
     1321 tgagaaactt gctgttgctt tatataagtt atcgagtaag gagcgtgatg taattctatt
     1381 acgctatttc caaagcatga gcgaccaaga aatcgcagaa ttataccatg tatcacgttc
     1441 tgctatttat cgcagaagaa gtaatggatt gaagaaatta aaaatagtat tgaaagaaag
     1501 gaattgaacc aatgaaaaaa agaatatggt taccctacat taaaagtaat atgtcaagcg
     1561 tctgctggaa gcgaaactgc cataagagag attttaaaat tttatgacgc ttatatttgc
     1621 aagttatgct tgcgtccgtt ttaccactct gaaagtggta aaatcactat gcaggttgat
     1681 gaagaactaa aaggtcaaat tcatacagaa atgatgaaag caatcttgaa atttgaaatc
     1741 agagtgaagt aatcatatta catgaatgaa aaacgggaga tacacagatg tttgtgctat
     1801 ctcccatgtt attttttgtg tcatatcaag gctcattcaa tcgtggtaaa ttcgtggtaa
     1861 aatgagtgtt tttctatact tcaaaatgtt tgaaagtata gtgttcatgc ggataatcaa
     1921 aaatcggata taaaatttaa aataaataaa tatgccaaaa aactgttttt acaaggtttt
     1981 tcctaatgta aattattttt tcgcatatat tcatttta
//
LOCUS       DAPCMT010000231         2011 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPCMT010000231 DAPCMT010000000
VERSION     DAPCMT010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.241"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            115..>2011
                     /locus_tag="RD743_002818"
     CDS             115..>2011
                     /locus_tag="RD743_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HDU0564581.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGA"
BASE COUNT          729 a          364 c          363 g          555 t
ORIGIN      
        1 aaataagaaa agccaaaatt atataaggct tttcttattt tttattgact atatcggtta
       61 accgatatat aataatcatg taaacatttt taattaaatt taggagttga aaaaatgaaa
      121 ttatttaatc caaaaactgt tttagctatt ggaacatttg ctgcctcact tagtcttgct
      181 ggatctgttg acgctgcttc aactaacgtc atgtatcgcc tttacaatcc aaataataat
      241 gaacattttt acacctctag caaaaaagaa cgtgaccatc tagtaaagat aaaatgggga
      301 aattatgaag gccccgcctg ggaagcaccg actagcggtg gccatcttgt ctatcgtttg
      361 tacaataaag gtcttcgtga tcaccattac acagctagtt gggatgaagt gaaatggttg
      421 actaaaaatt acggctggac ttatgaaggg cctgcctggc gttctgccga aaaaaataat
      481 aaaccgatct accgcctttt ccttcctggt gtaaaatctg gctcacatca ctacactgca
      541 agttggaaag aagtacaatg gttaaccaaa gactatggct ggaaatacga aggtatcggt
      601 tggtatggcg ctgacacctc taaacccaaa gtgaataaat cggctctaga agctctttat
      661 acgagcgtta agggaacgaa aaaaggtgac tataccgaca atacctggaa cgcattccaa
      721 accgctttaa acaatgctaa gaaagtgtta aatgactcaa aagcgactca aaagcaagtg
      781 gatagtgcca aaaataaatt agattctgct tacaaaggat taaagaaaaa accagttcct
      841 actgtgaata aagctgaatt acaagcttta tacaaccgag taaaatcaac tgctaaagga
      901 gattttacag aagaatcttg gaataatttt cagactgctt tatccaatgc taaaaaagta
      961 ctagacgatt ctaaggtttc tcaaacacaa gtaaacaatg ctaaaaatag tcttgatgct
     1021 gcatttaaag ggttgcaaca caaacctgaa acgccaaaag aatatatggt taccattaac
     1081 catattgaca atcaaacagg tcaagtgatt gctacagatc aagcaaaagt gacttctggt
     1141 tctacttaca ctgctaaggc aaaagcattt aaatatagcg aaggtgctaa agataatttt
     1201 agctacaaag tgattggtaa tgatactcaa agcaaaacaa tcacaggtaa tacaacaatc
     1261 acattcaatt ataatcaagt gcatcaaatc aatttgttgt gcaataacaa ctacaaaaat
     1321 gaggggcgag ttgatatttt gaaagaacag ataatcaacg tcactcacgg tgaaagtgtc
     1381 actttaactg ctccttctat tcaaggatat gttttagacg atcgggtaga accaacaaat
     1441 tcagtaacct taaacaatgt gactgaaagt caaaattata ttttcaacta cactcgtaaa
     1501 tttaatgtga ctgttaatca tgtaaatgct gataccaacg ctgttctctc tagtgaatca
     1561 aaaacagtat acgaagggga tagcttctct actccttgga aaaatatgac tgatcaaaat
     1621 tatttcttgt gcagaaatga cgatcctacc gttaccgtgg ataaaaacgg ccaacgatcc
     1681 attaataacg tcgatcaaaa taaaacaatc acattcaaat ataaaaagat ttcattagac
     1741 gaattacgta actttatgcg tcaaaaagaa cttgcttggt taaatgatta tcgccaacaa
     1801 aatggtgttg ccccaatgca attcaatgat attgttcaac aggctgcaga tattcgagct
     1861 aaagaattac aagtcagctt cagtcactat cgccctggtg gtggtacttt ccaagattta
     1921 ttggaaagct taggctgcta tggtgctaag ggagaaaaca taagtcaaac tgggctgtat
     1981 attgatgacc ttttatcaga cgatggtgct a
//
LOCUS       DAPCMT010000232         2002 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPCMT010000232 DAPCMT010000000
VERSION     DAPCMT010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.242"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..378
                     /locus_tag="RD743_002819"
     CDS             <1..378
                     /locus_tag="RD743_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDU0564582.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="RD743_002820"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="RD743_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDU0564583.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2002
                     /locus_tag="RD743_002821"
     CDS             1859..>2002
                     /locus_tag="RD743_002821"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564584.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tt
//
LOCUS       DAPCMT010000233         1995 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPCMT010000233 DAPCMT010000000
VERSION     DAPCMT010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.243"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            608..940
                     /locus_tag="RD743_002822"
     CDS             608..940
                     /locus_tag="RD743_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDU0564585.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1559..1900
                     /locus_tag="RD743_002823"
     CDS             1559..1900
                     /locus_tag="RD743_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDU0564586.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          691 a          270 c          421 g          613 t
ORIGIN      
        1 gcatactcct tttttttgag ttataataat acgcgaagag agataatttc attatttaaa
       61 caagctaaaa atcttgatat tacttctttt caggcaatac gaatattatt cgcttacatt
      121 ttttgtataa atttaattgt ctgctatgct tgtgatgaat tgattttcgt tctgcacttt
      181 tggatgataa atttggacaa ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa
      241 ggctggtaac cttcgtcagc gttcatacac ggcattaaga tttggccctt aatgcgttaa
      301 cctttcagtg ttacggtatt acccgcaatg gatttagtat aacagatatt caaaagtgat
      361 accacttttt ttggctgtgt taaggttaaa aatacgtgat attacgatga atctatgcct
      421 aagcacaatg ttataaaaat gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta
      481 gagggaacgc atgtttggat gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt
      541 ctctattttt gaaaaataac aagaaaaact tgttaaaaat atttaaagaa attgggtggt
      601 ttattttatg tcatatacag gaattttgag tctcaaagat atttgtcact acggtaaacg
      661 gtgtaccgct acagagaaaa tcacgaagaa actatcaact ggccaaagta aaaccgttgt
      721 gcagtgtaag aaatatataa tccagaaaga caaagtctca gaggaaatga tttattacat
      781 tgggaaacaa aagcaaataa ttttaaaaga tcctatcccc ttaaaagagc tatatccaac
      841 aatcaaacat gtttatgacc aaaacggtgt actgattggt cgaagaaaaa atggagtctt
      901 gcgttgtacc gcaaaaggga tggggcgttt gatcggctga attgatcatt ctcataactt
      961 gtattttatc gtttttcgct tgcttgaaaa tattgtcgag acttctaaag gaacttagag
     1021 tcactagaaa aataaggagt agacttgatc tatcaacaat tcccttcggg gagtgacggg
     1081 gtaggtttta acgattggta acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg
     1141 atagtggtga aatgaatttg tgtaaataat aagcgcagag taaacaagag ggaatttaga
     1201 agttagacaa gttagaatag cgtctagatt aatcatgagc agctactaag ggatctagac
     1261 tttgacgcaa actcatggaa taaggagtag gtcttctcgt aatggggagg acgcacaaga
     1321 aattgtgtcg tgtgctgcag tacatgtaga tacttgcgag gacgttgatt attggatcaa
     1381 tcgagcattt accgctgtct actagggttt ctcgtttcgg acgccataaa cgagtcgaaa
     1441 ttttttatgt atttttaagt aaacgaaaaa cgataaaata tgaacaagtc ctgaataaag
     1501 tgaaaaaatt gatataaaac atagagaaaa tgaaatttac gtaatggagg aatgacaaat
     1561 gaaaaattat gatccgaata tacgattggg cacacatact attaaggttt ctttccaacg
     1621 ttgggattac aaaggatttg tgacgtttag aagaggcgga aattgtaaag gattagatgt
     1681 tttagcatta gacgaggatg atttatacga tcaaacattg actgataatc caattggatt
     1741 cggcttgcta cctgaagatg atgaaggtaa tgaatggttt aaaatgacgc ttatgaatga
     1801 taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag
     1861 ctttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt
     1921 acagataaag gcttagaagg agtcacgatg attaataaaa aggtagtatt accttaggca
     1981 ttgagtaagg aattt
//
LOCUS       DAPCMT010000234         1931 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPCMT010000234 DAPCMT010000000
VERSION     DAPCMT010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.244"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            235..801
                     /gene="amaP"
                     /locus_tag="RD743_002824"
     CDS             235..801
                     /gene="amaP"
                     /locus_tag="RD743_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDU0564587.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            814..1002
                     /locus_tag="RD743_002825"
     CDS             814..1002
                     /locus_tag="RD743_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDU0564588.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1015..1572
                     /locus_tag="RD743_002826"
     CDS             1015..1572
                     /locus_tag="RD743_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDU0564589.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1604..1846
                     /locus_tag="RD743_002827"
     CDS             1604..1846
                     /locus_tag="RD743_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDU0564590.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          700 a          301 c          400 g          530 t
ORIGIN      
        1 ttgcaatttt taatcttgaa taatatcctt taagtatagc cagcaatcaa aagtaaatag
       61 agaataagta agcaattgtt atttcagtat cagactggca ataaaacgcc gttatgatcc
      121 agcaatgttt ggtagcgctt tatttactga tgctatgcta actgttcgct atctatactg
      181 tgattgtcgg tagagttatt tacagggtaa aaataagttt ataggaggtg gaaaatgaat
      241 aaggggttaa aaataatcgg tgttatcatg agtttactgc tactttcagt atttctagcg
      301 attgcgttga tcaatgcgcc gtatattatg cctgaacaat tggaacgatt tcgcttcttt
      361 actttaacga attactacat gcaacaatat attttctggg cagctgtagt atttgccgtt
      421 cttgctatta tcatcttgct agtcgttctt ttctatccaa agtcaagagg aactttcgtt
      481 atgaaacggg aagatggcaa actaacaatc gacaaaaaag cgatcgaagg gttggtacgt
      541 tcccatttac acgaagaaga gttcattcat tcacctaaag tacggatacg ttcaacgaaa
      601 aatcgaatcc atatccatgt caacggagac ttgaaacgaa cttcttcatt agtagggaaa
      661 acaggtgtat tgatgcaaga tatcgaagaa gaagtaacta aagtactcgg aacaaaagaa
      721 acggtcaaag tggcagtcac atacagcggt tatgaagaac aagaagatac aagagactac
      781 aagcactctc gagtggaata ggagggccaa ataatgaaag aactattttc agcatacaag
      841 ctccctatta tctttggggg tcttggacta ctgctagctg ttttatggtt aacgattgga
      901 tttttcaaga cattgttgat cataatcatg acagcaattg gcgcaggaat cggtttttat
      961 ttgaaacagt caggaatagt tgatcaattt tttaaatcat aggaggaaac aaagatggaa
     1021 aacaaaaaca tgaatacaac aaatggatca gttttggcaa atgaaaaagg agaaaaccgc
     1081 atgatgaatg gtgaattgac ttatgaagac aaagtcatcc aaaaaatcat cggttatgca
     1141 ttgaaagatg tagatggtct attgacatta gatggcggat ttttctcaaa tatggctgaa
     1201 aaattagtca acacagataa cgtaacagca ggtatcaata cagaagtagg taagaaacaa
     1261 gtagctgtgg atctagatgt gatcgtagaa tacggtaaag acatcgaaga tatttacaac
     1321 caaatcaaag aattgatcag tactgaagta aacaatatga cacatttaga tgttatcgaa
     1381 gtgaatgtga atgttgcaga catcaaaaca caagaagaat accaaaaaga tcaagaaaca
     1441 gtacaagaca aagtaacaga agcagcaaaa tccacaggac aatttgcttc acgccaaaca
     1501 gataaagcaa aatctgctgt aggcaaaggt gcacaaaaag tcaaagaaaa taacgaacca
     1561 cgtgtacaat aagaagtaag aagtcagtta ggaggaattt aaaatgggat ttatttgggc
     1621 attaatcgtc ggcggtatca ttggagcaat tgcaggagca atcacgaata aaggatcatc
     1681 aatggggatc atcgcaaaca tcattgctgg tttagtaggt tcagcaattg gacaagcttt
     1741 actaggaact tggggaccaa caattgcagg aatggctatc attccatcat tgatcggtgc
     1801 aatcatttta gtattagtcg tttcattctt tgcacgtaaa ttataagcaa attgaagaaa
     1861 aacttatgtt gaaatgaaga acaaatttga gaaccgtagc gacactcgct gcggttcttt
     1921 tttatatatt t
//
LOCUS       DAPCMT010000235         1928 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPCMT010000235 DAPCMT010000000
VERSION     DAPCMT010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.245"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(606..896)
                     /locus_tag="RD743_002828"
     CDS             complement(606..896)
                     /locus_tag="RD743_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564591.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1104..1796)
                     /locus_tag="RD743_002829"
     CDS             complement(1104..1796)
                     /locus_tag="RD743_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDU0564592.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          524 a          371 c          296 g          737 t
ORIGIN      
        1 ttgatgcaaa agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt
       61 tttcttagtt aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa
      121 gacatacaat catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa
      181 aatccaaatg ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc
      241 ttcaatttac ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca
      301 ggaacgttta aaatcctaca attccactac ctgagactag taatgactag tctcttttta
      361 tggcctctga tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc
      421 tttaagcaat atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa
      481 tacatgtagt tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc
      541 ccatcgtctg catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt
      601 gttttctagt ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca
      661 tattctacca caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt
      721 ctattattat aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag
      781 ttcaaactat gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt
      841 ctggcactaa cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc
      901 ttcacctcaa taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta
      961 tctttttgac tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat
     1021 gaaggacatg tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat
     1081 ttaggtttac ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat
     1141 ttatcagctg tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc
     1201 aagcttgagt tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgagtt
     1261 tctacatttg ttttttcttg tgtcagtgtt gcgatttgtt gattcaaacc atttacagta
     1321 ttttgtaatt ctgaaagttt tgcttgtgct tcttgtagtc ttgctgtcca atctgcattc
     1381 agatgattga tttcatcatt tttttggttg atctcatcta gtttgacatt gatttcattt
     1441 tgtttgcttt gtagttgtgc atccttgctg actaattgac tttctaagtt ttgtttttct
     1501 gctgccgatt gatttttttg gttttctaat tcatttactt ttgtttggta ttcttggaca
     1561 tctgcctttg aatcttgcaa tgtctgttct ttttgtgcta gtcgagcggt aagatctgta
     1621 agcgcttgct ctttatctgt caatgtatga tcaagctgtg tcagattctc attgattgta
     1681 tcaatatctg tctctccacc ccacaaaata atttcatttg cgtaccgcgt gcctgtgtag
     1741 ccaattagac atagaaatgc aacgacaatc ccaccagtaa taaatttctt tttcatttgc
     1801 atagctcctt aatccaatac ttttacatgc taaaagtaag tgatctcaaa cacaatgtca
     1861 agcgttttta aattacatgt gtaattttaa tataaatact atcaaatact catttaatcc
     1921 tccttttt
//
LOCUS       DAPCMT010000236         1919 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPCMT010000236 DAPCMT010000000
VERSION     DAPCMT010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.246"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            67..1533
                     /locus_tag="RD743_002830"
     CDS             67..1533
                     /locus_tag="RD743_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0564593.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          591 a          328 c          394 g          606 t
ORIGIN      
        1 aagtttgtta aaaattgaat atgattacat ctggataaca aatttttatc ataaaggaga
       61 gaacgaatga acaacaaaca attacgctgg tttactgttg cgttaattgc atttaacatg
      121 gtttggggca tggggaatgt agttaataac tatgctcagc aaggagtatc agttgtcacc
      181 tcttggctat tgattttggc catttatttt attccatacg cattgatcgt tggacaatta
      241 ggatcagcct tcaaggatag taaaggaggc gtcagttcat gggtcgaaaa tacgacatct
      301 aataaacgac tggcatacta tgccgcttgg acttactggg tcgtccatat tccttatttg
      361 gcacaaaagc ctcaagcaat tctgattgct ggtggttggg cagtccaagg aaatggaaat
      421 atcgtaaata cgatgtcagt acaaatggta gcagttattt cacttattat atttcttgcc
      481 tttttatatt tatcaacaaa aggactttct actttgaaag tgattggcgg actggccgga
      541 acagcgatgt tcgtcatgtc gatcttattt attcttttag ctgtaacggc accgttcatc
      601 aatccaacga tggaatttgc cacaccacat atgaatcaag tgaaaacata tattccaaca
      661 tttgatttca gttatttcac aaccgtttcg atgctagtat ttgcagttgg aggggcggaa
      721 aaaatatccc cttatgtcaa ctctacaaga aatcctgcaa aggaatttcc taaagggatg
      781 atcttcttag cagcaatggt agggatttgt gctgtattag gctcagtcgc aatgggtatg
      841 ctgtttgcta gtgataatat tcctaaagac ttgatggcaa atggggcata cagtgctttt
      901 caaattttag gtaattacta tggtatcggt aactcattga tgatcattta cgcattgaca
      961 aacatgattg gacaaatctc cgcattagcc ttttcaattg atgcaccact aaaaatcttg
     1021 ttagcagatg cggatccaga atttgtacca aactggctac gtaaacgtaa tcgtaaaggt
     1081 acattggtga atggttatct tctgacaggt atactagtag gaatcattat tttgttgccg
     1141 atttttggta tcaaagatat gaatgaatta gtaaaatggt tgacgaactt gaactcagtt
     1201 gtcatgccaa tgcgttacct atgggtgttc ttggcatata tgatgttgaa taaagcttat
     1261 aagaaattca attctgaata taaatttgtt aaaaatccaa aaatcggttt tatctttggt
     1321 gcttggtgct ttgccttcac tgcgtttgca tgtatcctag gaatggtgcc aaaagtagat
     1381 tatactgctg atcctaaagc atggtggttc caattattta cgaatatcat cacaccaatc
     1441 gtcttgatct tattaggtat gatcttgcca gcaattgctc gtcgagacaa aaacaaaaaa
     1501 atcactgaac cagaaacagt ggttcagcct taaaatagaa atacttgtta caagaggttg
     1561 tgacagaagt ggtcagcttc aagaaataag aaggaattta cgaaaattgc ttctcaaatt
     1621 tttgtgaagt ttcggcttgt ttttcgaaga agctacttct ggaacaccgt gaatcagagg
     1681 ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcccctcat
     1741 atttttcgtt tggctgggct tattaccgca gatgcaagct ttggaacacc gtttaaggaa
     1801 gaggttgtaa aatcagcgat ttgacctgag tattagagaa acaaacggaa aaatagctcc
     1861 tcatattttg ccgctttttg agctaatgct gaaggtcagc tgattgaaca ccatttatt
//
LOCUS       DAPCMT010000237         1907 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPCMT010000237 DAPCMT010000000
VERSION     DAPCMT010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.247"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..247
                     /locus_tag="RD743_002831"
     CDS             <1..247
                     /locus_tag="RD743_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0564594.1"
                     /translation="DNYMILIKGPKEAFELVQNLPEIKEINFGGVSKKSESKQYGKAV
                     FLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            261..1010
                     /locus_tag="RD743_002832"
     CDS             261..1010
                     /locus_tag="RD743_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0564595.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1010..1840
                     /locus_tag="RD743_002833"
     CDS             1010..1840
                     /locus_tag="RD743_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0564596.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT          600 a          256 c          382 g          669 t
ORIGIN      
        1 tgataattac atgattttaa ttaaaggacc taaggaagct tttgaattag ttcaaaattt
       61 accagaaatt aaagaaatta attttggcgg tgtgtctaag aaaagcgagt ccaaacaata
      121 tggaaaggca gtctttttaa ctcctgaaga attagaagcc acaaaaaaaa ttcttgatca
      181 agaagtgaaa atatttgtgc aacaagtccc atcttcaagt attgaaaatg tagattttag
      241 taagtaaggg gggaaaataa atgttattaa aagccatttt attaggttta gttggtgtgg
      301 tagctgttat tgactccagg ttaattggtc gacaaaatat tggtagacca ctaattttga
      361 gtacacttgc cggacttgtg ctaggtgact tgcatacagg gattgttctt ggggcatctt
      421 tagaattaat ttctatggga ttcgtctcaa ttggggcggc tggacctcct aatatgcaac
      481 ttggtagtat tattgccact gcttttgcaa ttttaactaa tagtagtaca gaaacagcct
      541 taacaatagc tatcccagtg gctgtagctg gtgaatttat gagtattatc atgcgcatga
      601 taattgctca atttggacat gctgcagata aagccattga aaataataaa tttagaaaag
      661 ctcaaattat tcatatttat tggtcattta tttttaatgc ttttgtttac tttattccaa
      721 tctttttaac agtatatttt ggtgctgacg ttgtggcaga tttagtagat aaaattccag
      781 aaatgattac agacgccttg actgtctctg gaaatatgct ttccgcatta gggtttgcaa
      841 tgctattaag cacaatgttg agtaaaaaat tatatcctta ctttattttt ggattcttta
      901 ttgttgccta ttcaggtctt tcactaattg gcgtgacaat ttttgcagct ctgattgcct
      961 ttgtaatgga tcaagttaaa tatggaaaaa gagaagaggc gcatggttaa tggaaaatag
     1021 tacagaaaaa aaacgtttaa tcacaaaaaa agatcttaat aagatgtgtc ttcgttcatg
     1081 gacaatcatg tgttcgtgga attatgaaag acaaatgcat atgggattta tgtatgggat
     1141 ggcgccaatt ttaaataaag tttataaaaa tgatgaagag aaaaaaagag aagcttatac
     1201 tcgtcatatg gcatttttta actgtacgcc tcaaatgact tcatttatta tgggactttg
     1261 tgcttctatg gaagaacaaa atgctcaagc agaagaagga aactttaata ctgaatctat
     1321 ctccatgatt aaaaccagtt taatgggtcc ttttgctgga attggtgatt catttttcca
     1381 aggtacttta agaattatta catttgggat tggattgtct tttgctaagc aaggtagtat
     1441 tttaggaccg attttagctg ttggcctttt tgctatacct tctattttag ctgtttttta
     1501 cggcggtaaa ttaggatatg agtctggcaa taaatacctt actaaattgt atcaagaagg
     1561 tcttatggat cgagtaatgc aatttgcttc tatcattggt ttggctgtag taggtggaat
     1621 ggtggctagc atggtgccaa ttgtcacccc tttaaaattc actacagggg gaacagaact
     1681 tgttttacaa gacttacttg attccattat tcctggatta ttaccacttg tatgtacttt
     1741 gggaatatat aaattgatta agaaaaatgt tagtacaaat atgctcttat taggttgtat
     1801 tttggcaggg tttgttttaa gtataatagg tatcttataa tgattaaggc tctaattttt
     1861 gatatggacg gagttttaat tgatagcgag attatgtatc aaaagtg
//
LOCUS       DAPCMT010000238         1827 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPCMT010000238 DAPCMT010000000
VERSION     DAPCMT010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.248"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            31..840
                     /locus_tag="RD743_002834"
     CDS             31..840
                     /locus_tag="RD743_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0564597.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            880..1377
                     /locus_tag="RD743_002835"
     CDS             880..1377
                     /locus_tag="RD743_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDU0564598.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1765..1827)
                     /locus_tag="RD743_002836"
     tRNA            complement(1765..1827)
                     /locus_tag="RD743_002836"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1799..1801),aa:Ile,seq:cat)
BASE COUNT          632 a          331 c          349 g          515 t
ORIGIN      
        1 agatatgtaa aaagaaagta ggaagataaa atgaaactag cagcgattga tttagacggt
       61 accttgcttg atcgaaatgg aaagatacca gaagaaaata tccaagcact tcggaaattt
      121 gatgaaaatg gcggaatcgt aaccgtcgct accggaagga acagcatttc tgctaaagat
      181 attttttccg aattaggaat cggtggctat ttgatttctt ccaatggttc actgattgct
      241 gaaatgaagg atggaaaaat ggcgaaagtt ctcagacgtt caaaaataga tagcgataca
      301 ctgagaaaag ccttcttcta tgctcaagaa gcgaaaatat ccatcattgc tagccgagaa
      361 acccaagatg atcagctgac attcgatgaa tcaaatctag tcaaagatga cccttattat
      421 gaacatttca atttacagaa ccattctttt gatcaaattt taaatatgtt ggaagatcct
      481 tctcttagct atcttaaatt agcattaaca gataagagtg aagaaaaact acagaagcta
      541 caaagagatc tgcaagcaga tggtatccaa tcagtattct ctgatccgca tttcttagaa
      601 atcacgccta caggtatcac taaagctcat tcacttgttt ttctggcgga ttaccttgga
      661 atcgaatcga gtgacatcat ggcttttggc gatcaagaaa acgacattgc tatgttgaat
      721 tacagcgggt tgagtattgc gatggggaat gcacagcgac aagtaaaaga aattgcaaac
      781 caagtaacac tgacgaatga agaagctggt gttgcttata ttttaaaaaa cttctattaa
      841 aaagaataaa atgacgatat gaatgtagga ggaaagaaaa tgttagttat tattgatatg
      901 caaaatcata ttcttgatcc aaacagcgaa ttttatattc cagagtcaga tcaattggca
      961 gaacgtattg ctcaaagatt agctaaagca cgccaaaata atgaatatat tctcttcaca
     1021 cgggatatac cagttgaact gaaggacaaa gaagaagaac gagaagattt gcaattgatc
     1081 cctcttcttt ctccacaaga aaatgaacga gtgattaaaa agtattactt tacgattcca
     1141 cctgaaactt tagctgaaat ccaggaaact ttatttgaaa gcaaagaaga acaaaaagag
     1201 atcgaagtag tcgggattga aacaaacttg tgtgtactct ctaatctgat aggcctgcag
     1261 agtgcttttc ctgaagcaga cttttttgtt gatccaatgt tagtctccag tagaaaacac
     1321 ggagaatccg ctttagagct gttgaaagat ttcaacgtct ctatcgtcga atcataaaaa
     1381 tatctatctt catttacgat atttaacaca caataaaaaa agcacgcaga actgttttag
     1441 tcctacgtgc tttttctatc aaagtagatt ggctttttta tgacacagag tgaaacggca
     1501 ggctcacgac ctctttctta aatggtgttc aatcagctga ccctcggtat tagcttaaca
     1561 aacggcaaaa tatgaggaac tatttttccg tttgttaagc taatactcag gtcaaaacgc
     1621 tgatttcaca acctcttcct taaacggtat tcaaaagctt gcatctgcag taataagaac
     1681 agccaagtaa aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg
     1741 cagctttttc acaacctctt atgtaccttg taggactcga acctacgacc ggacggttat
     1801 gagccgtctg ctctaaccaa ctgagct
//
LOCUS       DAPCMT010000239         1797 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPCMT010000239 DAPCMT010000000
VERSION     DAPCMT010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.249"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            30..1382
                     /gene="brnQ"
                     /locus_tag="RD743_002837"
     CDS             30..1382
                     /gene="brnQ"
                     /locus_tag="RD743_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDU0564599.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          310 c          353 g          631 t
ORIGIN      
        1 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
       61 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
      121 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
      181 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
      241 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
      301 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
      361 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
      421 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
      481 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
      541 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
      601 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
      661 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
      721 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
      781 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
      841 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
      901 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
      961 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
     1021 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
     1081 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
     1141 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
     1201 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
     1261 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
     1321 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
     1381 gataaaagag gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa
     1441 tggcttctca catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc
     1501 gtttattgag aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa
     1561 aatggcttct cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca
     1621 ccgtttattg agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc
     1681 tccatatact ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat
     1741 ttcttttata atatagattt tagacatata tatgatataa aaatagtttt cgcaaaa
//
LOCUS       DAPCMT010000240         1791 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPCMT010000240 DAPCMT010000000
VERSION     DAPCMT010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.250"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..393)
                     /locus_tag="RD743_002838"
     CDS             complement(<1..393)
                     /locus_tag="RD743_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDU0564600.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSP"
     gene            complement(436..1368)
                     /locus_tag="RD743_002839"
     CDS             complement(436..1368)
                     /locus_tag="RD743_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDU0564601.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          514 a          368 c          282 g          627 t
ORIGIN      
        1 tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt gaagaaccta acgtgccata
       61 agacgctccg gtaatcatca ctggtttatc cacaaacgga aaaataccat atgaaagcca
      121 atttaatgcg ttaatcaatg aagcaggaac cgcatggtca tactctggtg tgctgatgat
      181 tacgccatct gcttgttcaa tttttccagc aatttcttta acaacatttg gtagttctgt
      241 gttttcaggt ttgttaaata gtggcagctc cttgatttcg acaatttcga tttctgcctc
      301 atttacaaaa taatttttca taaactgtaa taattcacgg tttgttgacc gcgttgaatt
      361 tgttcctact aatccaataa atttcatctt cattctcttt cctttctaac atcgatttct
      421 gactgttcgc acgtactaaa ttttttcaaa tccttttgta tacaagaatt gattctcttt
      481 tgttacaact gctccctcga tacccggctg ttcttcaagc gctttcaaca cttctaaaga
      541 agatgctcca aataaacgtg tagtccagat ttctccatct acagaagaat cagacaaaat
      601 cgtcaggctt gctacgtcat ttttaactgg aaagccagtt ttcggatcaa tgatgtgatg
      661 atacactgac gaaccatttt gtagataacg ttcgtagatt ccagaggtaa ctaaagattg
      721 gttgtttagt ttaaccaccg caagcgactc ttcacgtgaa cctttaggat ttcgcaaacc
      781 aatacgccaa gcaccatccg aacgtcttgg tgattcacca aaaacaacga tatttcctcc
      841 taagttaact aaagcaaacg ctgcatttcg ctctcggcaa tgtgtaatta atttatctgc
      901 aatgtaacct tttgctaacg cccccaagtc aatcatcatt cctggtttgg ttaaaaaaac
      961 ggttttcatt ttttcatcta attgaatttg gtgtggatcc gttagcagaa gtaactcagt
     1021 tatctctgct tttgttggaa ccaatgcatc ttcaaatcca atgtgccatt tttgtacaac
     1081 cggaccaata gcaatattca gataactatt tcttgcacaa ctatgtgctt ttcctaattt
     1141 gatgagctca aaaagttcag gatgtacaat cacagcttgt cgtccagcag cttgattgat
     1201 ggccattaat tcagaagttg catcatgtgc gctgaagcga tgttcataaa cgtgcaacca
     1261 ttcgcctaac tctgctagta atcgactagg ctcttcatgc ccaacttgta atgtaattgt
     1321 tgtccccatc atttgccaga ttttcgtttc ttcgatcacc tgattcattt tgtaccttct
     1381 ttacttgaac tacggattac atgagtaatt agtttttatt tttgttactg ttttcacaac
     1441 cagatgatac gcctgctaga tttatttgtc aaccgatttt atatattttt ttatataata
     1501 aggtaatttt aatataaaat tttatattgt aaaaaaacaa ataagggaaa caaacaacac
     1561 atttttgtgt gttgcttatt tccctctaac tatcgttttc tatttgatgg ttaacctact
     1621 taaaataaat actgcaacga caaacggaga tcaagcatgc tttgtaacgt attttacgat
     1681 aattacgact ttattttatt actctatctt gctgccatac ccgtctatcc ctaatcatct
     1741 ttggtgaatt ttttcaaagt tagcataaca aaaataagta ttttttcctt a
//
LOCUS       DAPCMT010000241         1781 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.251, whole genome shotgun sequence.
ACCESSION   DAPCMT010000241 DAPCMT010000000
VERSION     DAPCMT010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.251"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            79..1068
                     /locus_tag="RD743_002840"
     CDS             79..1068
                     /locus_tag="RD743_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0564602.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1050..1511
                     /locus_tag="RD743_002841"
     CDS             1050..1511
                     /locus_tag="RD743_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDU0564603.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1561..>1781
                     /locus_tag="RD743_002842"
     CDS             1561..>1781
                     /locus_tag="RD743_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0564604.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPN"
BASE COUNT          640 a          232 c          335 g          574 t
ORIGIN      
        1 aaaatagtat tgcaaaataa agcaatttca tagataatag gtattgtaaa gttatatgac
       61 tttataatac ttattagagt gagggataaa gaaatgagac aactacacaa agaacagatt
      121 gaaacagcaa tggcagcgtt aaaggaaaag acaattgatc atgtatattt tacagcatgt
      181 gggggttcac aagccgtttt aatggctggt caatatctat ttgataaaga attaactgta
      241 ccttgtcatg tttatactgc taacgaattt aactatgcta taccaaaagg attatctgaa
      301 aattctttag ttatcacttg ttcccattct ggcacaacac cagaaactgt agaagcaaca
      361 aaaaaagcaa gagaatttgg agcaatgact atttgtttat ctaatgaaga aggatctcct
      421 ctatgggaag cagctgaata tcctgttcac tatgattggg gtaaagatgt tgatgctagt
      481 gatttaaata aaggaattct ttatggttta ttatttaata ttttacttgt tttagatggc
      541 tctaaaaaat gggaaacttg cttgaatgaa tttgataagt taaatgatct tgttattgct
      601 gaaaagagca aatttgaaat aactgctaaa gaatggggta aaaaccaaaa acgagataaa
      661 attatttaca caattggtag cggtatcaat tacggagaaa tttactccac agcagcttgc
      721 tggttcatgg aaatgcaatg gattaattct aatgctattc attctgggga atttttccat
      781 ggtccttttg aaatcactga ttatgatgta ccattcatct tagtgaaaag tattggagat
      841 acaagatttt tagatcaacg ggtagaagac ttcactaaaa aattcagtaa taaattaatg
      901 gttttagacc aagctgattt tgacttagaa tctatagata gtgtagcgaa agaatatatt
      961 gcagctgtac ttagtggcgt tgttattcgc caaatggtag aagcgattgc ttttgaaaga
     1021 ggccattcct tagatgttag acgctatatg tggcaaatga aatattaatt aataggaggt
     1081 tgcaaaagat ggcaaatcaa ttatttcaca ttgtgattac aacacattct accctttgtg
     1141 agggttattt aaaggcaaca aaattaattt taatgaatga cgccacaggt attagtgctg
     1201 ttccatttaa agaaaatact agtccagatg attttgagga gagtatgaaa gaaataatta
     1261 gtcagcatca tttgcaacct ctaattattt taacggattt aattggagga acacctaata
     1321 atgtttctat taaacagttg aacaacaata gaattcaaat tattgctggg attaatttac
     1381 ctcttttact ggagttatta atggcccagt cagctggtaa atctatagaa gaaatagatt
     1441 ttaaggaatt actgaataac tctcaaaaaa gtatggttca tattaattcg cttttaaaag
     1501 atgtcagtta aggtggcaat ttctattcta taaattttaa aaattgaagg aggaaataga
     1561 atgattaaat tagtgcgtgt agaccacaga ttacttcatg gacaagtaat attttcttgg
     1621 actaaacagt atgatattaa ccatattatt gtggctgata acaaagttcc aggagatcca
     1681 atgtctgtga tggctttgac aattgctaaa cctgctgatt gcaaattaga cattgttaaa
     1741 attagtgatg ttaaagggat tatggaaaca tccccaaatg a
//
LOCUS       DAPCMT010000242         1772 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPCMT010000242 DAPCMT010000000
VERSION     DAPCMT010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.252"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            29..>1772
                     /locus_tag="RD743_002843"
     CDS             29..>1772
                     /locus_tag="RD743_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDU0564605.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYC"
BASE COUNT          643 a          226 c          355 g          548 t
ORIGIN      
        1 tcttactaat aagtagagga gaaagaaaat gactataaaa gtagacttac gaaaaaaacc
       61 atatcattta gatgaagatg ctatttcttg gattgaagag acgattgatg ctatgacttt
      121 agaagaaaaa ataggtcaat tatttgttaa tatgggatct agtcgtgata aaaattactt
      181 aagtagtatg gtcaatcagt accatattgg agctgtacga tataatccag gaactgctga
      241 tgaggtatat gatcaaaact atatcctaca aacagaaagc aaaattcctt tattaattgc
      301 tgctaataca gaagcaggtg gtaacggagc ttgtacagat ggtaccgaag ttggtgtaga
      361 agtaaaaatt ggtgcaacaa atgatgcgaa atatgcttat gaattaggga gagtttctgg
      421 aattgaagca tcggctattg gatgtaactg gagttttgct cctattgtag atattaatag
      481 aaattggaga aatcctatca tttctagtcg atcttttgga caaagccctg ataaagtttt
      541 agaacttagt ttagcttata tgaaaggtat tcaagagagt ggaattttgc ctgcagctaa
      601 acatttccct ggtgacggta ttgacgaacg agatcaacat ctatctttta gtattaatag
      661 tttaagtaca gaagaatggg ataattcttt tggaaaagta tactcaggct taatagaagc
      721 tggattacct tcaattatgg caggtcatat ccatatgcca gcttattcaa aacattttaa
      781 tcttgatctg aaagatgaag atttacttcc tgcaacttta tcaaaagaat taatcactga
      841 cttacttaga ggaaaactcg ggtttaatgg attagtagtt actgatgcta gtcatatgtt
      901 aggaatgact ggggcaatga aacggtcaga attattgccc acttctattg cggctggatg
      961 tgatctattc ctatttttta atgatccaga tgaagacttt aattatatga tggaaggtta
     1021 ttacaaagga attatttcag aagagcgttt acgtgatgca ttaagaagaa ttttaggctt
     1081 aaaggctaaa ctaggtttgc ataaaaaaga aaagaataaa attttgttac cgaaacaaga
     1141 agctatggaa aaaattggtt taattgaaaa taaatcaatt tttaaagagg tagctgatca
     1201 agcaattaca ctagttaaag ataaacaaga tatttggcct attgtaccag aaaaatataa
     1261 aaggatttta ctggtagatg tacatggggt aaaaggtggg tttggtgaag tagttgctgg
     1321 aaatagacct caggccattg atttattgaa acagattttg tcagcaaaag gatttgaagt
     1381 tgaaaaatac atttcacctg aagaagaatt gaaaggatta tcgcaagaag aaaaaattaa
     1441 aaatatagct ggaatatatg ctgctaagag accaatcaaa gatttaactg aaaaatatga
     1501 cttgattatt aatatagcta atgttaaccc aggtacagtt caaagaattg tttggccagc
     1561 aagtaaaggt actccggata ttccatttta tattcatgaa attccaacaa tttttgtatc
     1621 tgtacagtgt ccgtttcatt tagctgatgt gcctcaagta aaaacttata tcaatactta
     1681 tgatggaaaa caagaaactt tagaaatatt agtggacaaa ctaatgggcc tttctgaatt
     1741 taagggaata agtccaatag atgcttattg tg
//
LOCUS       DAPCMT010000243         1759 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPCMT010000243 DAPCMT010000000
VERSION     DAPCMT010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.253"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            180..776
                     /locus_tag="RD743_002844"
     CDS             180..776
                     /locus_tag="RD743_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDU0564606.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(834..1316)
                     /locus_tag="RD743_002845"
     CDS             complement(834..1316)
                     /locus_tag="RD743_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564607.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1422..1619)
                     /locus_tag="RD743_002846"
     CDS             complement(1422..1619)
                     /locus_tag="RD743_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564608.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          588 a          356 c          270 g          545 t
ORIGIN      
        1 acaatgaaag cgataactaa gaaaatcctt acataacgat ttttcttatt aaaattgttt
       61 tttgaacttc gcttattttt tgtgagcata tctgtttact tactttttgt aaggtgctat
      121 acttcatttc gtaatcaatt aaaaaaatgc aaaacgcaaa tcaaaggaga aattttatta
      181 tgacaacttt tactgacact ttgaaaaacc gccgttcaat ctatcatcta ggacgtaatg
      241 tttcactttc taatgaagaa ttaacaactt tgatcaaaga agcaattaaa gaaagcccaa
      301 ctgctttcaa tgctcaatca acacgcgcag ttatcctttt cggagatgca catgaaaaac
      361 tttgggaaat cactgaagaa gcacttcgcc cattgactcc agcagaagct ttcccaaata
      421 cacaaaacaa attagctggt ttcaaaaatg gttatggtac agtattattc ttcaaagata
      481 ctgacgtggt aaaaggtcta caagaacaat ttgaattata tgcagacaac ttcccagatt
      541 ggtcagaaca atcaaacgga atcgcaacag ctaatacttg ggtagcatta gttgataaag
      601 gattgggcgc aaacttgcaa cattacaacc cagttattga cgaagcagtt gcaaaagaat
      661 ggaatatccc ttcaaactgg aaactacgtt cgcaattagt attcggttca ccagaaacac
      721 cggctgggga aaaagaatac atgaacgatg ccgatcgctt ccgcgtattt ggataatttt
      781 atttgaaata aaaaacagag gttgtgacaa acatcttgtc gcaacctctg tttttattct
      841 ggatcaatca taatatcaat ctctgcctca tcgtttaagc gttgggacaa aaatggaact
      901 aattcttcta atgatgcaga aaatttcagc atcggtcctc gccatccgtt tcggaaaaaa
      961 ttcattttta cgattttaga accttttggc tgatataata caattttatt gatctcagaa
     1021 aaaggaacac cttttttgtc aaagctattc aaaacaagtc cttcttctgt gattcctcta
     1081 ctgtccaaca aaaaactaat aaaaatcatt gctgacaaga tcccttttgc caattcatct
     1141 gctgtaggat gactgatgag tgtataccaa atgactagtg acactaagaa gggtatcacc
     1201 aagtagaccc atcgatttcg cacagtccga taaataattt cttttcgaac cagccaaaga
     1261 aataaaaaca tcacaacaac gaataaaaac aatagaaccg taaagaatgt taccatgaca
     1321 actcgcctcg ctttttcttc tccagaaaat aggaaaagaa tactgattta ccttctcaaa
     1381 gaaattggac ttgcagcacg ccgcacgtcc aattgttttc tttattcaaa tgtatcatat
     1441 tttgcaacaa ccatgcttgc tttttcatct aataattctt ggtcctctgc agcacattgg
     1501 gcaactttga ccacggcgca gtttgtcatt tttgatacaa cttcaccaat gaccgttttt
     1561 gtaaaaccaa ttggtttgca tgcatattgt tctcctaaat taatcatttc ttccgtcatc
     1621 aaaatcctcc taaactgctg gaacttttct ccacattacc actgctattt ctttggagat
     1681 ttaactcatt gatttttatt tacagttaaa aatcaacctt acaggcatta tagcgtactg
     1741 ttttcgtttt cacaattaa
//
LOCUS       DAPCMT010000244         1757 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPCMT010000244 DAPCMT010000000
VERSION     DAPCMT010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.254"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            318..932
                     /locus_tag="RD743_002847"
     CDS             318..932
                     /locus_tag="RD743_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0564609.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(956..1306)
                     /locus_tag="RD743_002848"
     CDS             complement(956..1306)
                     /locus_tag="RD743_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDU0564610.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1300..1563)
                     /locus_tag="RD743_002849"
     CDS             complement(1300..1563)
                     /locus_tag="RD743_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDU0564611.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          581 a          309 c          277 g          590 t
ORIGIN      
        1 gcaagattcc ttactaaatt agtttctgaa tggaaaagta ttatttctca aaatatggat
       61 aaattaagat attaaggatg ttagttttaa aataaactaa tgtccttatt tttgtgttca
      121 catttctgtt acctaagaaa tacttaaagg aggtgatgta gccagcaacg actacaactt
      181 gaacccgcta tctacagttt atttgttgtt tcttttgggt ataccctaac tagacacgac
      241 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      301 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      361 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      421 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      481 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      541 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      601 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      661 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      721 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      781 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      841 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      901 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      961 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
     1021 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
     1081 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
     1141 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1201 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1261 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1321 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1381 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1441 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1501 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1561 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1621 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1681 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1741 gaatcaggta agaaaaa
//
LOCUS       DAPCMT010000245         1730 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPCMT010000245 DAPCMT010000000
VERSION     DAPCMT010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.255"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(140..1288)
                     /locus_tag="RD743_002850"
     CDS             complement(140..1288)
                     /locus_tag="RD743_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564612.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="RD743_002851"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="RD743_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDU0564613.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          510 a          300 c          273 g          647 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg
//
LOCUS       DAPCMT010000246         1688 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPCMT010000246 DAPCMT010000000
VERSION     DAPCMT010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.256"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..998)
                     /locus_tag="RD743_002852"
     CDS             complement(<1..998)
                     /locus_tag="RD743_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDU0564614.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEA"
     gene            complement(1163..>1688)
                     /locus_tag="RD743_002853"
     CDS             complement(1163..>1688)
                     /locus_tag="RD743_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HDU0564615.1"
                     /translation="IPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQE
                     KKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQS
                     HLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHE
                     LYMKKGYENKTKRM"
BASE COUNT          497 a          311 c          304 g          576 t
ORIGIN      
        1 gcctccttca gggacgtaga aaagccaagc aatcgatcaa tcatcatcgc ttccgtaaga
       61 aatgggtatt ttggtgctcg aaaacttttc catgttttgt attcataatg gttgatgttt
      121 gcacgatttt ttagcaaaaa gcgccagttc tttttcagtt tttctgcctg acttttctgt
      181 cctactttac gaagttcatt catttcacgg atacgcaact cattaaaggc ttgattcatg
      241 tgtttgataa tatgaaaccg atcaatcact actttcgcat ttggcagaat acgtttggta
      301 agctggaagt aggcggcgtt catgtctgtg actaaaaatt ttacttcttc aggattagcg
      361 cagcctaaga aatagcttgt taatcgaggc aatttacgcg taggcaagac atcaattaat
      421 tgtcctgttt cgccatctgc gcaaataaag ctcattttat cttctatgga agcgtgcgaa
      481 cgaaattcat caaccatcaa tactctgggg agaatcctct tagattgctt tggtaagtag
      541 cttttaaact ctttcaacgt acgaataacg gtggtcaaag atacctgaca atttttcgca
      601 ataaaagata aagatacttt ttcagtcagt aaagaagcaa ttttatatct gacatgattc
      661 gcgattgaat gtctgggttg aacaaaataa ctttgagccg tccaatgggt tcgacagttt
      721 ttacaggtat agcgctgctt ttttaagcgc ataaccaaag gcatgtgatt gtattgttca
      781 aagcggacaa tcgtttcctt cttcccattt ttcactacaa ttactttccc gtttccatct
      841 accacagtag aaccacaatt tctacaggta tggggagcag gtgagaaaac agcatcgacg
      901 atcaacgtct ttttcttctg aaaggtctcg taagagacct ctgtaatcat caaatctttc
      961 tctattaatc gcagcatttt tttgatagaa tcattcatat agcatatcgt cctctcagtt
     1021 gtttattttg tcgtgattta atcatactag agaacgatat gttttttaat acctaaaata
     1081 aaaatggggc tgaagaatca attctgattc atcagtccca taaattatag agccggaata
     1141 agaggatgtg acaagaattt tgtcacatcc tctttgtttt gttctcataa ccttttttca
     1201 tatacagttc atgaaccgct tttattaact tgccaaataa cactgtattg tgcgttgtat
     1261 ccagatagaa agtctccgtt tcatctgaaa ttttctcatg ctgatctgta atgatcaaat
     1321 cagcatgttc tgaatatgcg gtaattttaa cagcatcttt gcgaaataac tgctgcaaat
     1381 ggctttgaag atgataattt cctgtaaact gtttggttat ctgcaaatgg atgagtaact
     1441 gctctttcgt ttctgtttgt agtaagtgtt ggatcaattg actaaggtat tgactgattt
     1501 ggtttctttc cccatttttt gaaaaaattg tatcgatttt ttctcttaat ttcttttcct
     1561 gtataaaaga actagaatga ttggacgtgg cttctggaaa gcaaagtgta gaaaaactac
     1621 tgctggattc acccaacagt tcgaaaaaaa caactgccaa cacaagaaaa taaaaataaa
     1681 ttggtatt
//
LOCUS       DAPCMT010000247         1673 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPCMT010000247 DAPCMT010000000
VERSION     DAPCMT010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.257"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..1402
                     /locus_tag="RD743_002854"
     CDS             <1..1402
                     /locus_tag="RD743_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDU0564616.1"
                     /translation="EGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPG
                     NYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNL
                     PALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKY
                     NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDG
                     SSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKE
                     KVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTE
                     PNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVS
                     SNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILL
                     GTLGLLGYKNRKKENE"
     gene            1536..>1673
                     /locus_tag="RD743_002855"
     CDS             1536..>1673
                     /locus_tag="RD743_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDU0564617.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TS"
BASE COUNT          659 a          251 c          362 g          401 t
ORIGIN      
        1 agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg ttactaactt
       61 gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta caattgatac
      121 caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg gtacgaaaga
      181 tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata aatatgaacc
      241 aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag ataatgttac
      301 taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat
      361 tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc cagacggtac
      421 gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg cagataaata
      481 caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg catctgaggg
      541 aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag agaaagtaga
      601 tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag atggatcatc
      661 tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag ataaatataa
      721 tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat ctgagggaat
      781 taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa aagtagatgt
      841 tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg gatctaaaga
      901 agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac ctcaagttga
      961 tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg cagaggtgac
     1021 agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg gtaattttac
     1081 agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa ctgatgaatc
     1141 tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag acagtggaaa
     1201 aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc aagacaaaaa
     1261 tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg acagtaatat
     1321 ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag ggtataaaaa
     1381 tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag aagaggaaga
     1441 atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt taaagatgaa
     1501 tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta attattggtt
     1561 caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca aattatgaag
     1621 ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca tcg
//
LOCUS       DAPCMT010000248         1636 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPCMT010000248 DAPCMT010000000
VERSION     DAPCMT010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.258"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..753
                     /locus_tag="RD743_002856"
     CDS             <1..753
                     /locus_tag="RD743_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDU0564618.1"
                     /translation="SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILL
                     VLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEIS
                     AGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHN
                     GFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMV
                     EAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            755..>1636
                     /locus_tag="RD743_002857"
     CDS             755..>1636
                     /locus_tag="RD743_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564619.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEK"
BASE COUNT          574 a          197 c          266 g          599 t
ORIGIN      
        1 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
       61 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
      121 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
      181 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
      241 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
      301 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
      361 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
      421 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
      481 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
      541 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
      601 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
      661 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
      721 atttcttact tgtatttaaa aaaggagaat taatatgaag acagctttca ttgcttggac
      781 tcctttacat ttgattaata tattaaatgt ttatcaaaat ttttaccgag aatcaaaagg
      841 agatttattt atttattctg aatttgaaaa cgctagaaga tatagtgagc gtataaaaga
      901 attagggcta tttaatgatg ttgtttttgt gaatcaccgg aaaatcggtt cttcctttaa
      961 acgtaaaatt aacttgttaa ctaaccataa tcaatttttc tcggatgatt tatccgttta
     1021 tgatgaaatt tttatacagg gggaaaatta tttcacaaaa atacttttct ccaatgttta
     1081 caaaaaaaat ccagatgtaa aactaaatta tatagaagat ggtttgggta cttatttgaa
     1141 gaagagtata ttttcaacaa gtaaaaacgt tcaaagagtt tttaaaatat taaataaaaa
     1201 tagcatcttc aaaaaaaaaa ttgacgatta ttttgtctat tgtccggaat tgttacttta
     1261 tgatgtaaaa aatataaaaa aaattcccaa aatagatgaa aacagtaaat tgatgaaaaa
     1321 catacaatca atctttggga tagaagaagt ttttgtcgga gataaatgta tcttttttga
     1381 tcaaccttta ttatctgatg gttatggcat agatgaaaca gatattttcg ctaaagtaaa
     1441 tagtatattt tctggaaaaa aagaaacttt aattaagctt catcctagag caaggacaaa
     1501 tcgttatggt gaaaaaaaca tattgaattc cagtttaccg tttgaagtct tgctacttga
     1561 aaatgacttc tcttcaacac ttttgattag tcctatatct acaatttctt tttcaccatc
     1621 tatgatgttt gaaaag
//
LOCUS       DAPCMT010000249         1607 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPCMT010000249 DAPCMT010000000
VERSION     DAPCMT010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.259"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..1019
                     /locus_tag="RD743_002858"
     CDS             <1..1019
                     /locus_tag="RD743_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDU0564620.1"
                     /translation="KLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFMDNEL
                     TDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLV
                     TQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVA
                     FCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDIN
                     YIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCH
                     SLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKH
                     DILI"
     gene            1075..1293
                     /locus_tag="RD743_002859"
     CDS             1075..1293
                     /locus_tag="RD743_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564621.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1364..>1607
                     /locus_tag="RD743_002860"
     CDS             1364..>1607
                     /locus_tag="RD743_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564622.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDV"
BASE COUNT          601 a          189 c          307 g          510 t
ORIGIN      
        1 aaaaacttca gatgggtgaa attgttagag gtcctagtca aatatctatt gatgttacat
       61 ataaatgtaa ttttagatgt aaacattgtt acaatgcgag tggtggagac aattgtttta
      121 tggataatga gttgactgat gatgaactgt tagaacttac aaaagatata tgtaatttaa
      181 agccatataa tatttgtttc tgtggaggag aaactttact gagaaaaaaa atagttttat
      241 cttgtgccac tttattaaaa gaggcaggta ttcctaatat tgctattgtt actaatggat
      301 cattagtaac tcaggatgtt gcaaaagaat tattcaaagc aggtattaat cgatttcaag
      361 taagtttaga tggaaaaaat gctagtagcc atgaagcact tagattatat ccaaacagtt
      421 ttgaaaaggc aattaatgct atcaaattat ttagagaatt gtccgaagaa gtggatgttg
      481 ctttttgtcc tactagtttt aattacaatc aattacgaga agtatatcaa ctagtaaata
      541 ctcttaaagt gaagtcttta agaattcaac catttatgct tagtggtagg ggaatgcaaa
      601 atagggaaat agtgccaact gtttctcaat atagaaaact agtttctgat attaactata
      661 ttaaaagaaa tgaagattat accaagatag attggggaga tccaattgat catatcatcc
      721 gacacagaac tttattgaaa gatttttcga attttgtttc tattaggtca gacggaggat
      781 taatggcatc accttacttg ccaattcaag ttggaaatat taaatgtcac agtctttcta
      841 aatattggcg agcaggactt tataaagttt tttctttaga tttagttcaa gatatagcta
      901 ctcaagtttc ttcaatacaa gatatgagta aagaattaaa aaaaggacat attaaatggc
      961 ttaatgaaga tatagaaatt gatattctcg atgaatacaa acatgatatt ttaatttagg
     1021 tttatcattg gaaagtttag ataaacaaag tacttttaga aaggagggaa aaaaatgatt
     1081 tctgaacctc aaatgaataa tatgtatgaa agcccaacta taatgggagc tggtgaagct
     1141 gcgccaagag cttgttcagc aattatggtg tgtgtgttat tagcatctgc aggagtaggt
     1201 atccaagcag gagcggtgta tacttatgct gtagttgcta cagtagctgt agctgcagcc
     1261 gcagtttgga aaacagtagg attagttggt taggaattaa taggagacta actgtctcct
     1321 attgattcca atgattaagc taaggaggaa ttttaagaaa ataatgaaat acaataattt
     1381 aaaaccgaaa ttacttaatg agaacataga atatataaga gaagaaaatt atggatattt
     1441 aaccatagtt cccaaaaatg gaactgaatt gaaagataac gttttcaatc caacagtaag
     1501 atatatattg gctaactgta ctggaaaaaa cagtgtaaaa aaaatagcga atggtttaaa
     1561 aaagaagttt cctagtttag aatatgaaaa attctatcaa gatgttg
//
LOCUS       DAPCMT010000250         1573 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPCMT010000250 DAPCMT010000000
VERSION     DAPCMT010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.260"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAPCMT010000251         1550 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPCMT010000251 DAPCMT010000000
VERSION     DAPCMT010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.261"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            4..411
                     /locus_tag="RD743_002861"
     CDS             4..411
                     /locus_tag="RD743_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564623.1"
                     /translation="MLSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELTPLDRD
                     GIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEK
                     RNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            421..624
                     /locus_tag="RD743_002862"
     CDS             421..624
                     /locus_tag="RD743_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564624.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            835..1419
                     /locus_tag="RD743_002863"
     CDS             835..1419
                     /locus_tag="RD743_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0564625.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          543 a          223 c          286 g          498 t
ORIGIN      
        1 tttattttat ctttaggtat tttaactatt ggaattagtg taactggaac tacaattcct
       61 acatttgctg atagtacaaa tgaaccaact actgtagtag ataataacca agaattaact
      121 cctttagata gggacggaat agccattggt aacccaacta acgacttaac tgttactgaa
      181 aattatttaa tagtttctcc attacttgct ctagcagctt ctaaaacgat acgagtgaca
      241 ggaacgatga tttttgataa agttggttgg ccttctagac ctcctcaatc acggaattgg
      301 aaagaaaaaa gaaatggtta ttggtactat ggaaaattgt atttacaaag ttataaatca
      361 gctggaaaca attggattgc gacttattca ggaaccttaa ctagaaagta gggatttttt
      421 atgccagaat ttgtgaacac accaaatttt tatcaagata gcgatattct aagtaaaatt
      481 aatttaccga aagaagaatc agctttacaa gttcagcgaa ctttacagga gacacaaaaa
      541 attcaaaaat caatttctaa tctagaagat tctgaagaaa aaagaaaaaa aagagaccaa
      601 aaaacatatc aaaaagaaaa ataatgattt taaaaaaaga gtgtctagtc gacattcttt
      661 ttttctaatt gaaagtgttc ataaaatcac ccatttctgg acactttctt tttttgcttt
      721 atttttcaaa aaaacaattc atttcatgtt cataaaccca taacaatatc tatgttcagt
      781 taaactatat ggataaagtt atgttacaat aaaattaatg aaggagtgat ttttttgaaa
      841 ataggatacg cccgggtatc tactggttta caaaatttgg atttacaaga agacagccta
      901 aataaatttg gttgtgaaaa aatttttact gatcatatga gtggggcaaa aagtaatcga
      961 ccaggattag aaatggccat tgattttgtt cgctctggcg atacacttgt cgtttggcgc
     1021 ttagatcgtt taggtagaaa tatggaagat ttgatttcaa tcgtcaatcg actgaatgag
     1081 cgtggtgtga gttttcatag tattcaagaa aatattacga tggacaaatc aagttctaca
     1141 ggacagctca tgtttcattt gtttgcggca tttgcagaat ttgagcgtaa tttgatcctg
     1201 gaacgatccg cagcaggacg agcagctgct cgtgcaagag gacggtttgg tggtcgtcct
     1261 gaaaaattgt ctgaacaaga tttggaatta ctgaagactt tagtggatag cggtacacct
     1321 attaagacta ttgcggaacg gtggaacgtc tcaagaacca ccatttatcg ttatttggca
     1381 aaaatggagg agaaaaatgg aaagacaaat tctcattagt ttttgtcatt taatgctgaa
     1441 aatttttgcc atttatctag aaaagtgaca atagtatgag aattttttgt aagtgtctac
     1501 tgataactta ttctcaaatg ttgtaaggat tttattcatt tgttaatatt
//
LOCUS       DAPCMT010000252         1532 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPCMT010000252 DAPCMT010000000
VERSION     DAPCMT010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.262"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            22..1488
                     /locus_tag="RD743_002864"
     CDS             22..1488
                     /locus_tag="RD743_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDU0564626.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          422 a          289 c          325 g          496 t
ORIGIN      
        1 taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg
       61 aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg
      121 cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact tggattacca
      181 cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc
      241 gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga
      301 gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt
      361 gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg
      421 gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac
      481 atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa
      541 tataactatc atttagggtt ctctttagct gctattggga tgttctttgg attattacaa
      601 tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg
      661 acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct
      721 gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca
      781 atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa
      841 gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca
      901 atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa
      961 cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat
     1021 ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa
     1081 cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac
     1141 ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg
     1201 ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg
     1261 tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc
     1321 ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga
     1381 acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta
     1441 tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata
     1501 aaaacaggaa ttgcttattt gcagcagttc ct
//
LOCUS       DAPCMT010000253         1509 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPCMT010000253 DAPCMT010000000
VERSION     DAPCMT010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.263"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            100..861
                     /locus_tag="RD743_002865"
     CDS             100..861
                     /locus_tag="RD743_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDU0564627.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(976..1416)
                     /gene="fabZ"
                     /locus_tag="RD743_002866"
     CDS             complement(976..1416)
                     /gene="fabZ"
                     /locus_tag="RD743_002866"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDU0564628.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          507 a          282 c          270 g          450 t
ORIGIN      
        1 tttaaaataa atgataaatt ccaataattt aagtaggtat tttccctcta ttttgttatc
       61 attaaagata acaaaatagt aaattggaaa ggaacagtta tgaagaaaaa taaagttatt
      121 ttaagtatta gtttagcagc agcattgctg tttgctgctg gttgttctgc aaacaacacg
      181 acttcttcta gttcgtctac agaaagttca aaaagtacat ctactgcgga aagcacgact
      241 gccagtagcg tcgatttgaa cgcattggaa ttaccacaat tgacgaaaga tgtcgccgaa
      301 aatgaagatc tggtcgaaat ggtgacgaat aaaggcacaa tcaaaatcaa attatttccc
      361 gaacacgcac caaaagcggt tgaaaacttt atgacacatg caaaagacgg ttactacaac
      421 ggactgactt tccatcgcgt catcaaagat ttcatgatcc aaggtggcga tcctaaaggc
      481 gatggcactg gaggggaaag catttgggga gaaggatttg aaacagagat caacaaccac
      541 ctttataata taagaggtgc gttatctatg gctcgctcac aagatcctaa cagtaacggc
      601 agccagttct ttatcgttca aaatacagat gatatgcatg atggtctttt aaaagacgac
      661 tatcctcaag caatcatcga cgcttataaa aacggcggtt atccttcact agatggccat
      721 tatactgtct tcggccaagt gattgaagga atggatgtcg tggatgctat tgcaaatatc
      781 gaaacagata gttcagacaa accaaaagaa gaagtaaaaa tcgataaaat caatatactg
      841 caagaagcaa aacaaaaata aaaaaataac gtctgaatcc tgcatagatg acaacgtatg
      901 ctaggattca ggcgtcttct ttttacataa aaagcaagta agcttccttt tgttttgcta
      961 tatcaggttt tatagtcaag cattttggtt gacgatgaac gtgaactcgc actcacagac
     1021 ttttttatca tctacatatg ctgcagataa cgctgtaccg actgatccct tcattttggt
     1081 gatttcaaaa tgacatttca gcacatctcc tggaacaact ttttgacgga atttggcctt
     1141 attgatccca ccgatataag ctctttttcc tagaaactct tctgatttta ggataagtat
     1201 cgaacctgct tgtgccagtg tttctagaat caacgcaccc ggcatcactg gattgtcagg
     1261 aaagtgacct tggaaaaaat cttcattgat tgttacattt tttgtcgcaa cgatcttttt
     1321 tcctggttct aaagtatcta cataatcaat ataacaaata ggataacgat taggaatcaa
     1381 ttccattact tcagtagcag ataatacgta agacataatt aaactccttc tttcaggcaa
     1441 ttgccatttt cattactttg actatcaaag tatcatattc ctaaatattt agcaatcttt
     1501 ttttaattt
//
LOCUS       DAPCMT010000254         1492 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPCMT010000254 DAPCMT010000000
VERSION     DAPCMT010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.264"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            131..505
                     /locus_tag="RD743_002867"
     CDS             131..505
                     /locus_tag="RD743_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDU0564629.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="RD743_002868"
     CDS             complement(619..1248)
                     /locus_tag="RD743_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDU0564630.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="RD743_002869"
     tRNA            complement(1382..1454)
                     /locus_tag="RD743_002869"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          507 a          226 c          261 g          498 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aa
//
LOCUS       DAPCMT010000255         1486 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.265, whole genome shotgun sequence.
ACCESSION   DAPCMT010000255 DAPCMT010000000
VERSION     DAPCMT010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.265"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..210
                     /locus_tag="RD743_002870"
     CDS             <1..210
                     /locus_tag="RD743_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDU0564631.1"
                     /translation="ARIMPSSEFLLGHSLQAFDEQGRLIDEEQVAKLRELFKDFLLFV
                     TITSQLQHAHQANKREAESFSWETI"
     gene            230..1450
                     /locus_tag="RD743_002871"
     CDS             230..1450
                     /locus_tag="RD743_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDU0564632.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
BASE COUNT          514 a          268 c          300 g          404 t
ORIGIN      
        1 gcacgaatta tgccaagctc agaattttta ttgggtcatt cactccaagc ctttgatgag
       61 caaggacgac tcattgatga agaacaagtc gcaaaattac gtgaactatt caaagatttc
      121 ttactctttg tcacaatcac tagtcaattg caacatgcgc accaagcaaa taaacgtgaa
      181 gccgaaagct tttcatggga aacgatttaa tcgaggggag caaacaaaga tgaaattaat
      241 aggtatcgtc ggatcagcag ctgaactttc atataaccgt aaattattaa catttatcca
      301 aaaacaatat catgaactat ttaatttaga aatcattgaa atcaaagatt tacctctgtt
      361 caatcaagat gatgatcaaa caaatagtcc tgctattcaa cagctaaatc gcaaaatctt
      421 aatggctgat ggggtaatta ttgctacgcc agaacacaat catacggttc cagcaggatt
      481 gaaaagtgca cttgaatggc tatcatttaa aatccatcca cttgaaaata aacctgtaat
      541 ggttgtgggt gcttcttact acgatcaagg aacttcacgc gcgcaattac acttgcgcca
      601 aattttggac gcaccaggcg tgaatgcatt agtgatgccc ggaaatgaat ttttattggg
      661 aaaagcaaaa gaagcatttg accaagaaga tcgcttgatt gatccacgaa ctgttgactt
      721 tttaggcagt acattagcaa gttttgtaaa attcattaaa gtcgtgtcta ccttagcagg
      781 gcctaaaaag cttccaccag aagatttgca tgcgacagga acaattgcta cgacaatacc
      841 cgatgttgat atgtcagatg atcattggtt ggaaaaagct tctgaagcag tcggtgccgt
      901 ttctggaaat acttacgtca aattaaatcg tgggttattg acagtggatc aaatcaatta
      961 ctttttagat tcgatgccga tggaattaac ttttgcagat aataataacc aatttttata
     1021 ttataatttg cgccaagaag cgaaagagat gttagctgct cgtgtgcccg gtcaggtagg
     1081 gaatccatta gccaactgtc acccagaaaa atcgtataaa aatgtagagt gggtaattca
     1141 acaactgcga agcggagcaa cagatgcggt acgtgttcat gtaccaaaac aagtgccaga
     1201 aaaatatatt gtgcataatt accaagcaat gcatgatgaa aatggagagt atgctggaat
     1261 caatgaatac attcttgatt tcaaaccaat cattgagtgg tatttacaac aaacgggtca
     1321 acaacttgtt ggaaatgaag atgcggtttc aagtgcatct gttcatggtg gaaaaacaga
     1381 tgcagtaacc agcgccagca cacacgatga aactgcagta gatgcagttt cgagtgcttc
     1441 tgtaaaataa aaaggttctg ttgcaaagtt ttaaataaag aataaa
//
LOCUS       DAPCMT010000256         1468 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.266, whole genome shotgun sequence.
ACCESSION   DAPCMT010000256 DAPCMT010000000
VERSION     DAPCMT010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.266"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(141..1433)
                     /locus_tag="RD743_002872"
     CDS             complement(141..1433)
                     /locus_tag="RD743_002872"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDU0564633.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          399 a          328 c          264 g          477 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctgaac
//
LOCUS       DAPCMT010000257         1460 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.267, whole genome shotgun sequence.
ACCESSION   DAPCMT010000257 DAPCMT010000000
VERSION     DAPCMT010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.267"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            139..>1460
                     /locus_tag="RD743_002873"
     CDS             139..>1460
                     /locus_tag="RD743_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564634.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRIT"
BASE COUNT          534 a          201 c          269 g          456 t
ORIGIN      
        1 tctcaaaaaa aagataaaaa atgagaataa ctgatgaaaa aacaagcata atccatcata
       61 aaatcagtat aggtgaaaag atgaactttc atctgcatat caagtaattg gtatgaggca
      121 ttttttgagg tgtaggatat gaagttggat taccttatag aagaggaaga tagagaaaaa
      181 ctgctgctag ctagatattt agaatttgag aaaaagaatt atgtagtgct gcctaagtta
      241 tatgaaacat taggtttgtc taaatttaaa acaagaaatt atatcataga attaaataaa
      301 gaatttactg aattcaaaac aaagccagag atccaaattt cagaagaagc agagatccat
      361 gtcgaacgtc tgaatttgac ggatatcaaa aatttaagaa gaatctactt tcaacgatca
      421 aaaattgctc gactgttaac ggagatcgtg gattcaaaag taacgatcga aaaatttgca
      481 gagaatcatt ttttaagcat atcaagagcc tatgtaaaaa gaagagaatt agtctctttt
      541 cttgaacagc atcaaataaa gttaaagaaa aacatattag ttggggaaga aataaacata
      601 cggaacgtat tatttggggt atatttcggg gtctttaatg gtttttctat gccatttaga
      661 gaaagcgtca tgaatgaagc aaagagtatc gtgcaatatt ttgaatatct gttcaattta
      721 aaactgtctg caacaaaacg aatcaaattc aatttgctaa ccactattag tttgttgagg
      781 gcctatggga atcaaccaat cggaaaacgt ttcttttcag atgaatactt taagagctca
      841 gaaggaatga agatcatcaa ggatttatca catatttctt ttcttcatat ccctaaagag
      901 cagatcattg atgagatttc ttatatcatg ttgtttttga tcacggaaga agaatttgaa
      961 ttagatggcg catttggact tgcggaagct cattttcatg atatccacat gattagtcaa
     1021 aaaatgtcag tggaattgat tgatcaatta ccattttcga ttgaaatctc tgaagaagaa
     1081 agactatata tacgaaaaga acttcgtatg ggtatgatgc atgtcaatcg aaaaaattgg
     1141 atattcgact ttgagatgac tagctttacg tctaaaaagc agctacagtt tttcttcgag
     1201 acgtatccaa tattcagtac agcaatccaa aaggtcatac aagagaataa acgctatttc
     1261 ttgggaaaag atgaacgttt catgattcgc ttattttatg attatctctt tttattggtc
     1321 ggtatttgtc cggtatcata ttttgaagca ccgattcatg tctgtattga tttttcgcaa
     1381 ggatctgctt ataccgatta cattatttct caagtgaaag gatttaaaaa ttacaatatt
     1441 gtgattgaga gtcgaatcac
//
LOCUS       DAPCMT010000258         1440 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.268, whole genome shotgun sequence.
ACCESSION   DAPCMT010000258 DAPCMT010000000
VERSION     DAPCMT010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.268"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            105..1406
                     /locus_tag="RD743_002874"
     CDS             105..1406
                     /locus_tag="RD743_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDU0564635.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAPCMT010000259         1417 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.269, whole genome shotgun sequence.
ACCESSION   DAPCMT010000259 DAPCMT010000000
VERSION     DAPCMT010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.269"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(150..1337)
                     /locus_tag="RD743_002875"
     CDS             complement(150..1337)
                     /locus_tag="RD743_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDU0564636.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAPCMT010000260         1406 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPCMT010000260 DAPCMT010000000
VERSION     DAPCMT010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.270"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            138..1145
                     /locus_tag="RD743_002876"
     CDS             138..1145
                     /locus_tag="RD743_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDU0564637.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1157..>1406
                     /locus_tag="RD743_002877"
     CDS             1157..>1406
                     /locus_tag="RD743_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HDU0564638.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFI"
BASE COUNT          550 a          175 c          256 g          425 t
ORIGIN      
        1 tgatacttca tattttaatc aatcaaatgt tgattcgtta cgttttgaca tcattattga
       61 taattagttt agttatatat tttttaaaga ttaagaatcg aattatagat gactataatt
      121 cataggagga gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt
      181 ccattggaag tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt
      241 atagtagagg tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt
      301 tgagattttt aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg
      361 tggatattat ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta
      421 tggaagctgt aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa
      481 ataatgtaaa acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca
      541 tgggtgcgac aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa
      601 aaacagaaac aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg
      661 ttattcctct ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg
      721 atatgacacg atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta
      781 tgaattcaaa atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt
      841 tagctcaatc attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa
      901 taaaagttgt aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg
      961 aaattgaacg ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta
     1021 ctgacgaact gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa
     1081 cagaattaat gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt
     1141 ggtaaggagt tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca
     1201 taatggaagt ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa
     1261 cgcggttaaa tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc
     1321 aaaatatcag aaggataatc ttaaagagga cagttctcaa tttgaaatgc taaaaaaatt
     1381 agaattatct caatctgatt ttatta
//
LOCUS       DAPCMT010000261         1394 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.271, whole genome shotgun sequence.
ACCESSION   DAPCMT010000261 DAPCMT010000000
VERSION     DAPCMT010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.271"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            40..1209
                     /locus_tag="RD743_002878"
     CDS             40..1209
                     /locus_tag="RD743_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDU0564639.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          500 a          253 c          229 g          412 t
ORIGIN      
        1 ttctttctgt aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct
       61 aaaaaaagaa aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc
      121 tctacaggaa ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc
      181 catgctcaat cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa
      241 aaaatcgatc aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat
      301 aatcgcatca tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat
      361 gatccttctc tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt
      421 ttaaaacaag tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca
      481 caaattgctg gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg
      541 caccgaactg cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt
      601 cagcacttaa aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata
      661 ttgactggaa ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat
      721 atcatcgaac ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc
      781 aatcatgcac tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa
      841 tctgaaacag agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat
      901 ttaaatacgc tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc
      961 tcattatggc gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat
     1021 aattatatcc tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa
     1081 gattcacagg atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg
     1141 cctactgctg atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca
     1201 aatagctgat atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa
     1261 aaagtactcg actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta
     1321 cagactttaa ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa
     1381 attattctaa gatt
//
LOCUS       DAPCMT010000262         1390 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPCMT010000262 DAPCMT010000000
VERSION     DAPCMT010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.272"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            82..222
                     /locus_tag="RD743_002879"
     CDS             82..222
                     /locus_tag="RD743_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564640.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            520..777
                     /locus_tag="RD743_002880"
     CDS             520..777
                     /locus_tag="RD743_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HDU0564641.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(748..1056)
                     /locus_tag="RD743_002881"
     CDS             complement(748..1056)
                     /locus_tag="RD743_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564642.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1134..>1390
                     /locus_tag="RD743_002882"
     CDS             1134..>1390
                     /locus_tag="RD743_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDU0564643.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSA"
BASE COUNT          516 a          234 c          254 g          386 t
ORIGIN      
        1 ttattaatct aataaagtca acgcagtttt ttaaaaagtt gttaaatttg tttacgaata
       61 taaaagaaag gagagaagaa tatggaaaaa acagtctcgg caaaaatcga agatttgaaa
      121 ttagacatac tgaaacaagc aaaagtggcg atggaacacg cggtagataa agaagactct
      181 gccatggttg cagccatagc agagatttta gctcacgttt agtcatcgtt tttacggtct
      241 tctggaagct gaccatagat aacagcataa tgaatatcta aataagcttg aacaatttct
      301 tttggcgaaa ttgattcgcc tttagtagat acttgtgatt cgtgatttag ccatgcaaca
      361 actaaatccg cagcgatttc aaacggcgtt acactttgtg tcagttgcca taaagaattt
      421 cacaatatgt atggtcgtgg taataatacc aaagagcaat ttgaggaatt tatggaaatt
      481 aaaaaataca aaaaatcatt tattaaagga ggctaaacaa tgaacaagga aatgagccaa
      541 acaatcaagg tacaaaaaat gatagatgat ctaacacatg gaattgatag ccaagccgat
      601 aaaatcatca aggaattgca aggacaaaaa gttaaggatg caaaaatgct tctcaagact
      661 atcaactttg aaatgaatcc aacaaaaaga aagctcgccg atgtgttgga agaaaagttg
      721 gcttccgcta tcaacgagca agaattacta tttgaaaccg atacttttaa cagctgatgt
      781 tttatagatg acagtttcat ccgaatctat atcatcgccg agagaaaaga atgttgcttt
      841 tgacaaaaag ccgataagac ctacaagcgg tcttgatgtt gcaagagtag ataccccaat
      901 atcaccttgc aaggtattca cgtaaatttg agaagaatag aacattccat ctttttcttc
      961 gatatttgac gcatcatcgt atccaacgac caaagtatcg gcaaatattt tgattttttc
     1021 tccatcaagc atagtaatta caaattctgg tttcataaat tttcacctcc ttaacaatta
     1081 tttcagcctg tcacactgat aaggaaatta taccaaagaa aggaatgaga aatatgaata
     1141 caccacaaat ttttaatttc gagcaaaacg aagttcgaac tattttagta aatgatgaac
     1201 catattttgt aggaaaagat gtagcagacg ttctaggata ttcgaatccg caaaaagcta
     1261 ttcgagatca tgtggacttg gaggataaga cgcagaacga ttcgttcacc gtcaatggaa
     1321 cagcagttgt tttgattaac gagtcaggcc tttacagttt aatcttaaaa tcaaaacttc
     1381 cctctgccaa
//
LOCUS       DAPCMT010000263         1389 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPCMT010000263 DAPCMT010000000
VERSION     DAPCMT010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.273"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            50..310
                     /locus_tag="RD743_002883"
     CDS             50..310
                     /locus_tag="RD743_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564644.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(672..911)
                     /locus_tag="RD743_002884"
     CDS             complement(672..911)
                     /locus_tag="RD743_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564645.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1197..>1389
                     /locus_tag="RD743_002885"
     CDS             1197..>1389
                     /locus_tag="RD743_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564646.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSST"
BASE COUNT          486 a          189 c          254 g          460 t
ORIGIN      
        1 atggtattat cattttgaat aatgaaaaaa cattaaggat ggatgaaata tgaataacga
       61 aactatcatt ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac aaaaagtggt
      121 tggagaagta atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg aatatcatga
      181 cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt
      241 ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt
      301 agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc
      361 actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag
      421 agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga
      481 actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat
      541 ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat
      601 ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga
      661 agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt
      721 accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc
      781 tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc
      841 tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga
      901 attcttttca ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta
      961 ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata
     1021 catatatgga gagggtgata tcccagttca aaccagatac attggttacc atctgtatta
     1081 acgattatgc ctctaaacgc tttttttatg aaaaaattta taagtgaaaa cgtaggtatg
     1141 atattatcta gaggtaattt ttttgggtat ttaaatagtt gctaggagga tagcttttgg
     1201 atttttacag taattttatc ttaataatag caatattatt acttctaaat atttggtttt
     1261 ttgataagag tagaaatgca gggataggtt ttagaaccaa acgaagtacg tcttctgaaa
     1321 aaaagtgggt atattcacag actatttttt acggaggggt tatttcaatc agtttacttt
     1381 cttcaacac
//
LOCUS       DAPCMT010000264         1384 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPCMT010000264 DAPCMT010000000
VERSION     DAPCMT010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.274"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            259..1299
                     /locus_tag="RD743_002886"
     CDS             259..1299
                     /locus_tag="RD743_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDU0564647.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
BASE COUNT          476 a          155 c          320 g          433 t
ORIGIN      
        1 tacatgaaaa tcattatttc attgatggta tgaagtctag aggcttaata tgggatggga
       61 tatttggatt aggcaaaagg tttttagata gggatttttg gatttggttt ataattgcag
      121 ttgtagttgt gtgcataatt tgcatggcta tcgatgttgt aagagaaaaa tttgaaagac
      181 agatttttaa aattaagagt gtaaagaacc tgtgtaaact tatgaatcat gttataaata
      241 tatacccata attaataaat gaggagaaaa gaagtgaaag ttatatcatt cggagaatta
      301 ttattgagac ttgcagcacc cggttattca aagttatttc aaagagatag tttggagact
      361 acattctgcg gaggggaagc taatgtagcg gtatccttag caatatttgg atatcaatca
      421 gaatttataa caaagcttcc ggattcagat gttggatatg cggcagctaa tgctttgcgt
      481 tattttggag taaatgttga taatattata tttggagagg gacgcatggg actgtattat
      541 ttagaaaaag gagcagctca gagaccatct aaagtaattt atgacagaaa attttctgca
      601 atttcgttag ctgatagtga tgaatttaag tggaaggaga tcctaaacga tgcaaattgg
      661 tttcattgga cagggataaa tccagcattg tctgagaatc tctttgagac gtccttagat
      721 gcatgtcagg ctgccaaaaa aagaggtata acaatttctt gtgatttaaa ttatcgatcc
      781 aagctttgga catcagagga agcaaaagaa aagatggaac agatactacc attggttgat
      841 atttgtattt gtaatgaaga agatgcagaa aaagtgctag gaattaaagc tgagcataca
      901 aatattaata gtggagaatt aaataaagat ggctatgtgc aggtggctag ggagattcac
      961 aagaagtttg gatgtaggta tgtagctaca actctgcgta gaagttattc ggcaagtaga
     1021 aatggttgga gtgcaatgtt atacgaagca gaaaaagata aagcgtattt ttccacagaa
     1081 tacaacatac aaattgttga tcgcgttggt ggtggagaca gcttttctgc tggtataata
     1141 tatgcgttat gtgaaaatat gtccggacaa aaggctattg aatttgcgac tgcatctagt
     1201 tgtttaaaac atactataga aggtgatttt aatcgaacta caattgagga tgtatataat
     1261 ttaatgaata gcggtggaaa tggtcgcgtg ttgcgttagt caacatataa agataaatca
     1321 cagtaatttt tatggtgatt atctgtctta gtagtgatta gaataacgtg aaaataaatt
     1381 gttg
//
LOCUS       DAPCMT010000265         1382 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.275, whole genome shotgun sequence.
ACCESSION   DAPCMT010000265 DAPCMT010000000
VERSION     DAPCMT010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.275"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(943..1356)
                     /locus_tag="RD743_002887"
     CDS             complement(943..1356)
                     /locus_tag="RD743_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDU0564648.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          509 a          230 c          207 g          436 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 tt
//
LOCUS       DAPCMT010000266         1381 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.276, whole genome shotgun sequence.
ACCESSION   DAPCMT010000266 DAPCMT010000000
VERSION     DAPCMT010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.276"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..>1381)
                     /locus_tag="RD743_002888"
     rRNA            complement(<1..>1381)
                     /locus_tag="RD743_002888"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          286 a          415 c          271 g          409 t
ORIGIN      
        1 actcctcttc agacttactg cttggacaga catttccgat cgtctgcatt ccttagcctc
       61 ctgcgtccct ccattgctca aacaaaaaca acgggtacag gaatatcaac ctgttatcca
      121 tcgcctacgc ctgtcggcct cggcttaggt cccgactaac cctgggcgga cgagccttcc
      181 ccaggaaacc ttagtcattc ggtggacagg attctcacct gtctttcgct actcataccg
      241 gcattctcac ttctaagcgc tccagcagtc ctcacgatct gccttcaacg cccttagaac
      301 gctctcctac caatacacct aaaggtgtac tccacagctt cggtaatatg tttagccccg
      361 gtacattttc ggcgcagggt cactcgacta gtgagctatt acgcactctt taaatggtgg
      421 ctgcttctaa gccaacatcc tagttgtctg tgcaacccca catccttttc cacttaacat
      481 atattttggg accttagctg gtggtctggg ctgtttccct ttcgactatg gatcttatca
      541 ctcacagtct gactcccgga tatgaatgaa tggcattcgg agtttatctg aattcggtaa
      601 cccgagatgg gcccctagtc caaacagtgc tctacctcca tcattctcaa ttccgaggct
      661 agccctaaag ctatttcgga gagaaccagc tatctccaag ttcgtttgga atttctccgc
      721 tacccacacc tcatccccgc acttttcaac gtacgtgggt tcggtcctcc agtgcgtttt
      781 accgcacctt caacctggac atgggtagat cacatggttt cgggtctacg actacatact
      841 caaacgccct attcagactc gctttcgctg cggctccgtc tcttcaactt aacctcgcat
      901 gcaatcgtaa ctcgccggtt cattctacaa aaggcacgcc atcacccatt aacgggcttt
      961 gacttgttgt aggcacacgg tttcaggttc tatttcactc cccttccggg gtgcttttca
     1021 cctttccctc acggtactgg ttcactatcg gtcactaggg agtatttagc cttgggagat
     1081 ggtcctcccg gattccgacg gaatttctcg tgttccgccg tactcaggat cctcctaggt
     1141 gtcttccaca tttcgtctac ggggttttta ccctctttga ctgacttttc caagtcattc
     1201 gactatctga aagaactacc atattggagt cctacaaccc caacaagcaa gcttgctggt
     1261 ttgggctttt cccgtttcgc tcgccgctac tcagggaatc gaattttctt tctcttcctg
     1321 caggtactta gatgtttcag ttctctgcgt ctacctcgaa tgtgctatgt attcacacaa
     1381 t
//
LOCUS       DAPCMT010000267         1359 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPCMT010000267 DAPCMT010000000
VERSION     DAPCMT010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.277"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            44..658
                     /locus_tag="RD743_002889"
     CDS             44..658
                     /locus_tag="RD743_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0564649.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(823..>1359)
                     /locus_tag="RD743_002890"
     CDS             complement(823..>1359)
                     /locus_tag="RD743_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564650.1"
                     /translation="IITACEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYF
                     NIEFPKEIRKEIKNFIEKRNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFM
                     YRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQD
                     NKDFVENIDKEPTNTVGD"
BASE COUNT          450 a          238 c          216 g          455 t
ORIGIN      
        1 attagggtgt attttaaata gttgcagaaa gtaggtaaaa gttttgagag ttgcatacat
       61 acgtgtctca tcgatcgatc aaaatgaaca acgccagatt gaagaaatga aaaaattcgg
      121 tgcagagaga attttcatcg agaaacaatc tggcgcaacg attactcatc gacctgtttt
      181 ccaagaagca ttagattacg tccgagatca agatattttt attgtggaag ctattgatcg
      241 acttggaaga aattatgacg agatcatcgc ttcagtcaat tacttgaaga agaaaaatgt
      301 gaagctgatc attactagtt taccgatcat ggctgaagca attggcaatc ctctcctgga
      361 taaattcatt aaggatctga tcatccaaat tttggccatg atcgctgaac aagaacggac
      421 tgaatcgaaa cgtcgccagg cacaaggaat taagatcgca aaagcaaacg gcgtctacaa
      481 agggcggccc aaattataca gtgcagacgc aaaagatccc caacgtcgcc ttgtttacaa
      541 aagcatcgtt gaagatctta aaaatggtgt tgcgattgct aagattgcta aagactacaa
      601 tgttacgaga caaaccgtgt atagaataaa aaaagacagc atggtcaatg acaaatagtt
      661 gatcatgctg cttttattat cttctcatct ttaatttcct acatataatt actatctata
      721 aaattaagtc ttactcagaa cagataaagc ataaaattcc aacaaaccat tttttactta
      781 actaaataaa gagtctgtta atataatctt cacttattta cgttaatccc ctactgtatt
      841 tgttggttcc ttatctatat tttctacaaa atccttatta tcttgtgatt tagcttcgta
      901 taattctttg tatgtcgaca cattggttat actaattcct atagctagtt cttgagactc
      961 tacttttctc tctagctcct ttgacaaagt gatttgagat ttatttggtt ccgttggagt
     1021 caattctata gattcttcat aaaaatctct atacataaag ctatcattaa ttttataaaa
     1081 aaaattaaat gtttttagca ctgccatata acattcaaaa cattcttcta tagttggttt
     1141 acttccttga ggagaatgaa ccaatttatt acgtttttct atgaaatttt taatttcctt
     1201 tcttatttct ttcggaaact caatattaaa gtacccttta aacatgctcc ctaataattt
     1261 gtctacaggg ggagattgta aattctcaaa tattagagtt aaatcaggtt tctcttgttg
     1321 ataaaattcc tttaccccta tttcacaggc tgtaattat
//
LOCUS       DAPCMT010000268         1355 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPCMT010000268 DAPCMT010000000
VERSION     DAPCMT010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.278"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..1355
                     /locus_tag="RD743_002891"
     rRNA            <1..1355
                     /locus_tag="RD743_002891"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAPCMT010000269         1352 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPCMT010000269 DAPCMT010000000
VERSION     DAPCMT010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.279"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            400..1311
                     /locus_tag="RD743_002892"
     CDS             400..1311
                     /locus_tag="RD743_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDU0564651.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          541 a          197 c          259 g          355 t
ORIGIN      
        1 atttttggta gttttaatat attcattttt agttgtataa aaatatatca taataaaaac
       61 aacaaaaaaa tgtctggaaa atcgaacata aaagtgtctg atttgaatat cccagcagcg
      121 gaagaagaaa aacaaaatcg ataacatttg ttctaagaat tagttacgtt aaatacggaa
      181 cgcaagatag acttgaaaaa cggtaacaaa agaagtagta tattaaggtg tgatgtgcta
      241 cttgacattt attctgctga gcgaactttc tggattgtgt agccttatat ctattcagga
      301 cgtatcgagc agattagtaa aggtgttctt gatgattttc atcttgaaca cctttgtttt
      361 tagcaattcg caaaaaacga aaagtaggag aattgataaa tgaaaaaaag atttttagca
      421 cttgccatcg tactaggcac tggcttattg tcaggctgta cgaatgccgg tgaaaaaaca
      481 gccgttagtt ataaaggcgg tacaatcagc gaacaagaag taatggatag cttgaaaaaa
      541 atgcaaggag cagattccgc tgtgcaacag ttgatcgttt atcaagtatt tgaagataaa
      601 tatggagatg acgtatcaac caaagaaatc gattctcaat atgatcaaac aaagaaacaa
      661 ttgggtgatt catttgattc acaattgaaa tctgcaggtt acacagaaca aacattcaaa
      721 gacagtatca aacaaagtct agcctttcaa gaaggtctaa agaaacatat caaattgacc
      781 gatgaagatt taaaaacggc ttgggaatct ttccatccag aagtggaagc tcaaattatc
      841 caagtagcaa gtgaagatga tgcaaaagat gtgaagaaag ctgctgataa gggagacgat
      901 ttctctaaat tagccaaaga caagtcaact gatactacga caaaagaaga tggcggaaaa
      961 gtgaaatttg attccactac tacaacggtt ccggcagaag tcaaagaagc agcctttaag
     1021 ttgaaagatg gtcaagtttc tgatgtgatc acttctacca atgcttcaac ttatactacg
     1081 gaatattatg tggttaaaat ggtgaaaaac caaaacaaag gcaacgacat ggataaatac
     1141 aaaaaagaat tgaaagaaat tgcaacagac acaaaattaa gtgacagtac cttccagaat
     1201 aaagtaattg gcgaagtatt gaaagacgct aatgtaaaaa tcaaagacaa agactttgaa
     1261 aatgttttga gtacattcac ttcagattct agtacagcaa gtagtaaata agcggtataa
     1321 ataaaaaata tgaactattt aaatcaataa aa
//
LOCUS       DAPCMT010000270         1320 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPCMT010000270 DAPCMT010000000
VERSION     DAPCMT010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.281"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..848
                     /locus_tag="RD743_002893"
     CDS             <1..848
                     /locus_tag="RD743_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDU0564652.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          241 c          256 g          394 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaattcggtt
//
LOCUS       DAPCMT010000271         1294 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPCMT010000271 DAPCMT010000000
VERSION     DAPCMT010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.282"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..1244
                     /locus_tag="RD743_002894"
     CDS             <1..1244
                     /locus_tag="RD743_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDU0564653.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAPCMT010000272         1284 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPCMT010000272 DAPCMT010000000
VERSION     DAPCMT010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.283"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..727
                     /locus_tag="RD743_002895"
     CDS             <1..727
                     /locus_tag="RD743_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HDU0564654.1"
                     /translation="IDFLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYG
                     LPVLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIP
                     VGYSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNT
                     EIFLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLY
                     QYIGKKVKRPISADSVLLEEDVE"
     gene            724..>1284
                     /gene="neuC"
                     /locus_tag="RD743_002896"
     CDS             724..>1284
                     /gene="neuC"
                     /locus_tag="RD743_002896"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDU0564655.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSF"
BASE COUNT          425 a          169 c          274 g          416 t
ORIGIN      
        1 aattgatttt ttgtctactc cttttgatga agaaagtgca gactttctaa aacaaattgg
       61 tgtacaagga tttaaaattg gctcaggaga tttaacaaat ttaccttttc ttagaaaaat
      121 agatatgtat gggttgccag ttttattatc tacaggtatg gctgatattc atgaggtcag
      181 tgaggctgta gattgtttca tagatagtcc agttactatt ttacattgta cttcaaatta
      241 tccggctagt tctgaagata ttaatttatt ggcgattaac acattaaaag aaatgttcgg
      301 gatacccgtt gggtactccg atcattcatt gggatatgat gttggtattt gtgctgttgc
      361 gatgggtgct aaggtaattg agaaacattt taccttggat aaggaactac ctggtccaga
      421 tcataaagct tctttagatc ctcaggaatt tgcagatttt gtaaagcata ttagaaacac
      481 tgaaatattt ttaggtgatg gtgttaagcg agcaatgcct tctgaactta gtacaaaaga
      541 agtatcaaga aaaagcctag tggtcactga gaatttaaat gtgggagata ttcttacaga
      601 gaaatctctt acaataaaac gtcctggaaa tggaatagct cctaagtttt tatatcaata
      661 tattggcaaa aaagttaaaa gaccaataag tgcagatagt gttctgcttg aggaagatgt
      721 tgaatgaaaa aaatacttat agtaagtgga acacgagctg attatggaat atataaacct
      781 gttgctgaga atttactaca agataattgg gatgtatcat ttgttgtttc tggaatgcat
      841 ttatcccata aatatggatt tactaaagaa ttgatcttag ctgacggatt tagaattagt
      901 ggagaagttc aaagtttgtt tttagagaac aataagggaa acatggctcg gtctgtggca
      961 ttagagataa atggtttcac aaatatattt gaacaaacta gccctgattt tgtattatta
     1021 ctaggggata gaggagaaat gttggcagct gcgatagctt gtttgtatct tggaatcaaa
     1081 acagcacata ttcacggagg cgaagtgtct ggtactgtag atgaatcaat aagacatgcc
     1141 ataacgaaaa tagcatctgt tcattttgct gctacggaaa aaagcaaaga gcgtttaata
     1201 aaaatggggg aagattcttg gagagttttt cccgtcggag ctcctagaat agatactatt
     1261 ttaaataata gattacctag tttt
//
LOCUS       DAPCMT010000273         1276 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPCMT010000273 DAPCMT010000000
VERSION     DAPCMT010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.284"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            161..1201
                     /locus_tag="RD743_002897"
     CDS             161..1201
                     /locus_tag="RD743_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDU0564656.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          513 a          179 c          208 g          376 t
ORIGIN      
        1 actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt taattaaaaa
       61 acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa gagtaggtaa
      121 ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt ttaaattttt
      181 taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct ttattgtttc
      241 tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt taaaagcaag
      301 attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt attttactta
      361 tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa
      421 aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta acaaacagtt
      481 agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag aaaatgatat
      541 ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat cagagggtat
      601 gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg
      661 tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa tcataccaga
      721 acttaataat aatatattag acactaatag acacaatata gacactgaaa aagaccgcct
      781 acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca gcattgctac
      841 gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt acagtgaagc
      901 tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag aaagtggtat
      961 ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag aattatacac
     1021 gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct
     1081 gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta aagtatcaag
     1141 tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact ggttagagtg
     1201 aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta aaaatttttt
     1261 attattctaa ttatat
//
LOCUS       DAPCMT010000274         1246 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPCMT010000274 DAPCMT010000000
VERSION     DAPCMT010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.285"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(349..705)
                     /locus_tag="RD743_002898"
     CDS             complement(349..705)
                     /locus_tag="RD743_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDU0564657.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(719..1129)
                     /gene="spx"
                     /locus_tag="RD743_002899"
     CDS             complement(719..1129)
                     /gene="spx"
                     /locus_tag="RD743_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDU0564658.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          399 a          229 c          175 g          443 t
ORIGIN      
        1 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacaactcag ttatcttatc
       61 atgtcatttc taacatgtca acaactattt tatatgtttt ttaaaatact attttattgc
      121 tttgctactt aaaacaacag aaatgaatat atcatgtaaa tttacacatg tcaatatatt
      181 tttaactcta tatccccacc tagatttgaa ttttgaccac ataaattgag catgtctcta
      241 tagtagaaaa ataaagtcaa aaaaagaaca cataacacgc ttgatgctaa gtgttcgact
      301 gttacaactg tatgaaatgc tcagtgactt gattaaaaca gttaaagtct atttatttaa
      361 gataagctgt tccatcaact gctcattgta tccaataaat agaacttcat cgataataat
      421 caaaggacgt ctgaggagat tggtatgttg tgtgcataaa aaaatcagtt gtttttttcc
      481 tacacaggaa aggtcaatcg caatgttgtg aaaagcctca gaccaagtcg caaatagctg
      541 ttctgcttgc acttcttcaa aactgcataa ttcttctgct tcttctgtac ttagatcgcg
      601 ttcttctaag tcaacaaatt gataaggcaa ttgatggtgt tctaaccatt gtttcgcctt
      661 cagacttgtt acacagtgcg ctgatccata gactttcact tccacgaagg ctccctccct
      721 atgaatttaa gatgttattg ttttgtaaat agatcagatt tctggcaaca catcgaattt
      781 cacgagggac aaatgaccgg atctcatctt cattaaatcc gattagtatt cttttttcat
      841 caatcaaaat aggtcgtctc aataatgaag gatattcttt gaataagacg atcatttgct
      901 ttaaagttaa ttcgttaaaa tctatgccca gcttttcgta aattttcgac cgagtggaaa
      961 taatatcttg aactcctcct tcagtcgctg ccaagatctt tttgatctca ctttctgtta
     1021 acggttcggc aaaaatattc ttctcttcaa attcaagttg attcgcttgc aaccatgctc
     1081 ttgcttttct acaggaagta cagctaggcg aagtatatac ttttatcatt ttctcactca
     1141 tttttaatta taatccaatt ctacagtaat ttcttttaaa ataccaatta ttagctaata
     1201 atatataaaa aaaaatcaaa ataactgatt atttacgttt gaataa
//
LOCUS       DAPCMT010000275         1243 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPCMT010000275 DAPCMT010000000
VERSION     DAPCMT010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.286"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            226..>1243
                     /locus_tag="RD743_002900"
     CDS             226..>1243
                     /locus_tag="RD743_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564659.1"
                     /translation="MKNTIFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESK
                     SFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACL
                     YRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNI
                     YTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYE
                     LVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMT
                     INSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVK
                     IAESL"
BASE COUNT          485 a          140 c          217 g          401 t
ORIGIN      
        1 cacttatcca gacactcaaa tgatttttct cttagtacct atgatagttt cgtgggttga
       61 taatcaacaa cgaatatcgg aatagatagc taaagtatat tttaattatg aatttataat
      121 tagttgtaaa agttaatatt taacggacag gaagtagggt gaaaaaattt gaaaaaaatt
      181 ttagtgttag caaatacata ttatcaaata atagtagcaa tacaaatgaa aaatacaatt
      241 ttttccgatg agtatgttga aattattatt tctgatcatt ctaaaaacac taaagaaatt
      301 gcgaaaaaaa tagaagaaaa aaaagtgttt gatggctgtc attatttaga aagcaaatct
      361 tttgatgata atattgggta ttttcaaaag attaaggact gtcttcagat ttcttgtggt
      421 aaatcaaatc gatatgccta ttatttgaaa aactgtaaaa gtgaatattt tgatgaattt
      481 atttgttttt caatgggaga ggtttggttg gaaggaatat atgcttgctt atatcgaaat
      541 aataaaaatg tacaggtatc aattttagaa gaaggtattt taagctatgg agttgacatt
      601 attccaacag caagaacaaa agctatcgca gtactcaatt acttaaaaaa aaggactcca
      661 ctatataaca aaagctataa tttttattgt ttttttccta atatatatac aggaagagag
      721 aatacaatac aaattccatt agttacagct gggtcaagaa ccgcattgca actttcggat
      781 atttttaacc ctatgataga tatttataaa gaaaaaaaat atatattttt tacgtctgtt
      841 tatgattttg agggcggaga acctgtcgga gaatatgaat tagtatgcaa aatagcagac
      901 ttagtaggaa aagataatct aattgttaag acgcatccta gagatgtaag aacaatttat
      961 aaagataatg gatttatcga ggataaaaat tcatcaatac cttgggaggc gattcaattg
     1021 tcaggcgatt ttaacgataa aatatttatg acaattaatt caggaagtgt tatttcaggt
     1081 agtacaatgt ctgcaaaacc tgtacgcacg tattatatgt ataaattgtg taacatagaa
     1141 ggaaatccgt cttgcaaaaa gaatgcgtat gatattgagc gattgcttaa gcaaaaaacg
     1201 atgaaaaaca ttttaaagaa tgtgaaaata gcagaaagct tag
//
LOCUS       DAPCMT010000276         1233 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPCMT010000276 DAPCMT010000000
VERSION     DAPCMT010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.287"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(76..1170)
                     /locus_tag="RD743_002901"
     CDS             complement(76..1170)
                     /locus_tag="RD743_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDU0564660.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          363 a          264 c          194 g          412 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agt
//
LOCUS       DAPCMT010000277         1220 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPCMT010000277 DAPCMT010000000
VERSION     DAPCMT010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.288"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            191..445
                     /locus_tag="RD743_002902"
     CDS             191..445
                     /locus_tag="RD743_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDU0564661.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="RD743_002903"
     CDS             446..763
                     /locus_tag="RD743_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDU0564662.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          430 a          185 c          238 g          367 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt
//
LOCUS       DAPCMT010000278         1208 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.289, whole genome shotgun sequence.
ACCESSION   DAPCMT010000278 DAPCMT010000000
VERSION     DAPCMT010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.289"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..384
                     /locus_tag="RD743_002904"
     CDS             <1..384
                     /locus_tag="RD743_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HDU0564663.1"
                     /translation="SNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVV
                     RDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMR
                     DELSELAKKVKVTFLTDPRWVRIDK"
     gene            519..1097
                     /locus_tag="RD743_002905"
     CDS             519..1097
                     /locus_tag="RD743_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564664.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
BASE COUNT          425 a          125 c          227 g          431 t
ORIGIN      
        1 agtaatttaa gacgttttca tgaattaccg aaagaaagca gaactaaatg tattgttaca
       61 tcaaatatac aagcatcgga tgtatattta cggacgagtt ataaaaagtt gttatctgaa
      121 agggaagttg taagagacaa tgcaggatta atggcaatta gatttttagc cgatatgaat
      181 gtggatgaaa tattcttagc tggatttgat ggatattcac ataatgaaga tgagaattat
      241 ggtgagcaat ccatggaaat tataacaaaa tttgcattat tagatgcaat gaacgtgcaa
      301 atgagagatg aattgtcaga attagctaaa aaagtaaagg tgacattttt aacggatcca
      361 cgttgggttc gaattgataa gtagtagtaa taatgttttt aaaattttga taaagagata
      421 gtgagagaat aaaaatgggt aaagtcttaa ataaaaaaga agaaaaatat ttaagggacg
      481 atggggcgag atgaaactct aattttgaac ttttaagaat catttctata ttgtgtattg
      541 ttgcttacca tgctaatttg cgtttaaatt ataatgcaga cggaattata gtacatggtt
      601 cattaactgc taaccagatt ttcttgaatt atattggtat ttggggtcag ctaggagtta
      661 attgttttat atttatttcg gcatattttt taagtgagaa aagaaacatt cactttgaaa
      721 atgttttggg aattgtatta gaatgtattt tttatagtgt attagtttat ggaattcttt
      781 gtgcatgtgg aatagaaaca ttttcattga acagctttgt aaaagaaata ttatcatttg
      841 ctctaggtag atattggttt attacatcct atttagcatt gtatattcta agtccgtatt
      901 taaatatcat gcttaacaat ctggaaaaac ttcagttgca aaaaataagt atgttattgt
      961 ttataggtgt atgctgttat cgtatgttat ttaaaacatc tttgtttggg gattgtgctg
     1021 ttttaattgc actatatgtc atatgttata cactgaaaaa aacgataata atatttttga
     1081 gagacacaga atcataggaa caattgtatg tataacagtt ataccacttc tcatatccat
     1141 aggaatactt ttcaaattta taccaaatgc aatggttaac cagatggttc atattggttc
     1201 tccattga
//
LOCUS       DAPCMT010000279         1176 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPCMT010000279 DAPCMT010000000
VERSION     DAPCMT010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.290"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..383
                     /locus_tag="RD743_002906"
     CDS             <1..383
                     /locus_tag="RD743_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDU0564665.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
     gene            complement(367..960)
                     /locus_tag="RD743_002907"
                     /pseudo
     CDS             complement(367..960)
                     /locus_tag="RD743_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          357 a          235 c          207 g          377 t
ORIGIN      
        1 catttagccg tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
       61 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      121 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga
      181 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      241 ttgactatgt aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      301 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      361 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      421 gaggaggctc attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat
      481 aaaatttgtc aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc
      541 ttcaatgtcc atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa
      601 tctttggatg acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg
      661 aattgttgaa gccatacgtt cttatatggg tttatcaata actgttaccg gtacaacttt
      721 taagttgagt ctttccttag caacacgata cctatgaagc acatcaacca agatatactt
      781 ctcttctttt tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga
      841 aagctccaat aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg
      901 tattttggta attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat
      961 acgcttgtat accctacttt cttaaaaagt agggtatact caagaatccg attaattatc
     1021 ttctcaattt ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc
     1081 tgcaaaatgg gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa
     1141 tttgtatcct aaaaaatcca taaaaattct ctcaca
//
LOCUS       DAPCMT010000280         1161 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPCMT010000280 DAPCMT010000000
VERSION     DAPCMT010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.291"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(257..1129)
                     /locus_tag="RD743_002908"
     CDS             complement(257..1129)
                     /locus_tag="RD743_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0564666.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          317 a          277 c          225 g          342 t
ORIGIN      
        1 atttgagtgt aacataaatg gcttttttat ttgtctcgct taattataca aacggcgtgt
       61 aaaaaactat tgcacaatac aagtggccta cggctatcga aatttccata actttcgagc
      121 aagaatttat ctcattcaag gcttgatctt ccaagtaaaa caaaaaccag ttaagcactc
      181 tgcctaactg gttatgacta aattttcagt tgtatctttc tctaccaaca ctaaatgtcg
      241 aagagccttt tttcgtttat gaatgtaaga ccgcttctcg agccaatgcc aaacaaccga
      301 gcgttccagc gttgtcttct aatgctgggg tcacgatata tttgcttaac tcaggtgttt
      361 cgacataccc attgaccaat tttgcaaatg cgtcgtggac gttttgcagc atatggcgtt
      421 gtttcatcac accaccgcca aaaatgatca catctggaga aaacatcaag gttgtattgt
      481 aggcacattg agcaatatag gaggcttcga tttcccaata aggatcatct tctgcaacct
      541 cttgtccttt tttccctaag cgtttttcga tggcgggtcc agcggccatc ccttctaaac
      601 agttgccatg aaatggacag ctgccagcaa agtcatcttt tggatgaggg acgacgagca
      661 tatgccccat ttctggatga ctaaaccctt cgataaagcg cccttcttgt aaggcaccgc
      721 cgccaacacc cgtaccgatc gtatagtaaa cgacactgct caatcctttg ccactgccga
      781 agacatactc accataggca gccgcattga catctgttgt ccaagaaatc gggatcggaa
      841 attctttttt catggtaccg atgaaatcaa aattttgcca tgccaatttc ggggtcgagg
      901 taatgtaccc ataagtggcg gaatcgcgat ggatatcgat aggcccaaat gagccaatcc
      961 caatccctgc taaatctgct tggtattgtt tgaagaagtc aatgactaaa ggcattgttt
     1021 cctcaggtgt cgttgtcgga aagctcactc tttccttgat cgtcatttct tcatcgccaa
     1081 tggcacacac aaattttgtg ccgcctgctt caattgcacc gtatagcata gacatttctc
     1141 cctttatttt agaatgaaat c
//
LOCUS       DAPCMT010000281         1143 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPCMT010000281 DAPCMT010000000
VERSION     DAPCMT010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.292"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="RD743_002909"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="RD743_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDU0564667.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DAPCMT010000282         1136 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPCMT010000282 DAPCMT010000000
VERSION     DAPCMT010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.293"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(91..1017)
                     /gene="cysK"
                     /locus_tag="RD743_002910"
     CDS             complement(91..1017)
                     /gene="cysK"
                     /locus_tag="RD743_002910"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDU0564668.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT          303 a          268 c          177 g          388 t
ORIGIN      
        1 caaataattt ttctcatcat aaatttttgt gtgttctttt catctttaaa agttttttac
       61 ataaacctat ctggagaaaa accaagtgcc ttatatctct tggtaaaggg ctgtagataa
      121 ataacgttca ccattgtcgg gaaccacagc taataccttc ttgccttttc ctaattcttt
      181 tgcgactttt agcgcggcag caattgcagc gccagatgag atgcctacta aaatcccttc
      241 ttttcgccca acttctctag ctgtttccat cgcttcgtct ccactaatag aaaggacttt
      301 atcatacaca gaagtatcca gtgttttagg aacaaaacct gtaccgattc cttggatttt
      361 gtgaggtccc gcttctttcc cttctaaaat agcagattct gctggttcta ccccgattaa
      421 ttcgattttc ggatacacac gttttaattc tcggccagcg ccagtgatcg ttcctcctgt
      481 accgatacct gcaacaaacg cgtccaagcc atttactcca aatgcttgat gtatctcagg
      541 accagtcgtc ttctcatgca caagaggatt tgcttcattt tcaaattgta atggtaaaaa
      601 gtagccgttt tcttttgcca aacgttctgc ttctctgatt gatccagaaa taccatccgc
      661 accaggagtc aaaatcaact ctgcgccgta ccctttcatc aataaacgtc tttcgatact
      721 catcgtctca ggcatcacga tgattacttt atatcctttt gcaactccta ccatcgacaa
      781 tccgattcct gtatttccag aagtaggttc aatgatagta tcacctggtt tcagtagacc
      841 atcgtgttct gctttttcta tcatacttaa agcaatacga tcttttacac ttcctcctgg
      901 attaaaaaac tcaagcttta caaaaacatc tgctgaatct tctggtacga ttttatttaa
      961 tttgacgatt ggcgttttgc caatcaattc ggtgactgag ttatagatag ctgtcatctt
     1021 caattcctcc ttgtctttgc tcttcttact tatcataata caatctgttc atttatggcg
     1081 agtgattcgt ctttgactag caaaaaaaag acaaagatat accatatatg gtaatt
//
LOCUS       DAPCMT010000283         1133 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPCMT010000283 DAPCMT010000000
VERSION     DAPCMT010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.295"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..819)
                     /locus_tag="RD743_002911"
     CDS             complement(<1..819)
                     /locus_tag="RD743_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HDU0564669.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIA"
     gene            complement(825..>1133)
                     /locus_tag="RD743_002912"
     CDS             complement(825..>1133)
                     /locus_tag="RD743_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDU0564670.1"
                     /translation="FDVLMLPSLFEGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTN
                     FISLNQSAKEWAREIANVDISHRDSERSILIKADYNIQTAYKILESKYYELFELIQRG
                     "
BASE COUNT          351 a          213 c          138 g          431 t
ORIGIN      
        1 tgctatttca tgatttattc tctgtgctaa aacaatcata taatctatca tcaaataatc
       61 caccatcata tcattatttt tccaataatt taaaaaaaag tctctaatta tagcaaacat
      121 ccatctagac tcttgattac atgctaatga ataacccgca aaatatcctg atgctacgga
      181 tgcgtgagca taatcaggcc tcctaataga ccaaatatcg atatcaaaat actcatctgg
      241 tattggtttt gtacaataaa atgtggcgtc taaccacaaa cctccatgtt ttgctagcaa
      301 agataatctt aataaatcag aataatttgt tctcgtaatg attcctttgt ttttcttttc
      361 ttccacccat tttggtatat caacatattg tctataatta tcttctgtta aaattataac
      421 agtatgtttc cctgcatttc gttttattga ttttacacaa gcttttacaa tttccggcga
      481 ttgatccaat ccctgccacc aacaaaccca aatgcagtta gaatatggtg attttgattg
      541 attaattatt tgattttgac tataatcata tgttttcaaa aaatctgaaa aagtcttttc
      601 ataatacttc agcattacct catgtttttt taataaatgt tccttctgtt ttttactttc
      661 attatatcca ttacgattca tgacttgaat atcaatttta gcttgtaacg tggcaaatgc
      721 ttccttccaa gatgttatct ttgcaattcg tcttgttgca ttcaattctt cggatatgcg
      781 atgtataatt ctttttatac ttgaatgttt ctgtgtcata ttctttaacc tctttgtatt
      841 aattcaaata actcataata ttttgattct aagattttgt atgctgtttg aatattataa
      901 tctgccttta ttaaaatcga acgttctgaa tctcgatgag agatatctac atttgcaatc
      961 tctctcgccc attcttttgc cgattgattt aaacttatga aattggtctt atcactgaaa
     1021 gcaacttctg taggaacttt ctccgaaaag aaacacggta aatctgcaaa ttgagcttca
     1081 atacccacta acggaacccc ttcaaataat gatggcaaca ttaatacgtc aaa
//
LOCUS       DAPCMT010000284         1108 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPCMT010000284 DAPCMT010000000
VERSION     DAPCMT010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.296"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            74..1021
                     /locus_tag="RD743_002913"
     CDS             74..1021
                     /locus_tag="RD743_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDU0564671.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          354 a          170 c          236 g          348 t
ORIGIN      
        1 ttgtttccta aatttttaga agattcttcg gagcttcaag atatctataa ttatttatat
       61 cggaggtaaa aggatgagct gttatcttat caccggtggg gctggcttca ttggttctac
      121 cttagccaat catttaggag aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg
      181 aaaagtagaa aatcttgata tgacgaaaaa tatcactttt attgagggag atgtcgctga
      241 gaaatccttg atggaagaga tcatgaaagc taataaattt gattatatct ttcatttagc
      301 agcagttgct agtgtggcag attcagttgc ccgaccagta gaaacacatc gtgtgaattt
      361 tgaaagtgta ttaatgattt tagaattgat ccgcaagtat caacctgatt taaaacggat
      421 cgtgtttagt tcttcagcgg ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga
      481 gtccgtgatt cgcccattga cgccttatgc ggttgataaa ttcgctgcag aacaatatgt
      541 gctagattat tgtcatttat atgatgtacc cggtagcgca gttcgttttt tcaatgtcta
      601 tggtccaaat caaaatccga attctccata cagtggtgtg atttctattc tcgtagatcg
      661 ctataagaaa caaatcgtag gagaagcaac ttcatttaca ttatttggtg atggaagcca
      721 gtctagagac tttgtttata tagatgatgt aatccaagca ttacttttag ttgcgaatga
      781 ggaagctgca ctggggaaac aattcaatgt tggaacgggt aaagcaacca cacttttgga
      841 attgattcat gcaatcaatg aaattctcgg tgtagaattg acacttgaat acaaagaaga
      901 gcgttcgggt gatatccatg actctctagc agatattact aagatccagt caattggcta
      961 tcaaccgaaa tatggtattt taagtggaat gactaattat ttgaaaactg aaatcaatta
     1021 aaacatacta agataagatt agagggtaaa ttcaggctct ttgtcaataa ggactgatga
     1081 gttttgtaaa atcaaatctg ggatagaa
//
LOCUS       DAPCMT010000285         1088 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPCMT010000285 DAPCMT010000000
VERSION     DAPCMT010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.297"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            42..584
                     /locus_tag="RD743_002914"
     CDS             42..584
                     /locus_tag="RD743_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564672.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            740..>1088
                     /locus_tag="RD743_002915"
     CDS             740..>1088
                     /locus_tag="RD743_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDU0564673.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          462 a          114 c          214 g          298 t
ORIGIN      
        1 aaaaattata tttcatcaac gtttcataaa aggtgtgtag aatgttattg aaagtagcta
       61 taaaatgcta ttgtgtaaaa aataaaggag gaataattta tgaaaaaata gtaccaagag
      121 gagcatgcct attacttgca ttatgtttga gcttaagcgc agttgggtgt caaaaaagta
      181 atgaaaacaa taaaataaaa gaaggacaat ccataagttc tgaagaggaa ggcatgtcaa
      241 ttaatgcaaa tggcaaaaat gaaactttta agccatcaga ttatacattg gaggcaaaga
      301 aagaatatgt ttatgaatat ttagggctta aatttaaact ctcagacaaa tttagaaatt
      361 atatagctga taaaaagata gcgatgttag atgatcaaag tcctatagat aaagaactaa
      421 agtatgctat tttgacattt gagaaaatga cagaagaaca aaagaatgca gttatagaaa
      481 agatgggcga tggatataaa aattggcaaa atgagcttga aagaattggg actattggta
      541 tatttgaaaa aaatacatca gaagaaaaaa atctaaaatt ataaaatgtg atactcatac
      601 taaaatagga gtttcaagtg atggaaaata cgattgctat ttaagcacaa atagcggagc
      661 tgaaagtaat cttctggatg aattaaaaag aactgagatt caaattatag ataaaaaaga
      721 acgtcctaaa aatggttttg tgctatcaga aaaaactgat ttagaaaata cagaagcatt
      781 taacaaagaa tcggttaaag atttacgtaa actaccaaca aaggacataa atggcaagga
      841 ttttacaagt aaagattttg agaagtatga tcttactatg gtaaatgtat ttgcaacttg
      901 gtgtacagct tgtgtaaagg aaattcctga tttggttgag gttcagaatg aaatgaaaag
      961 caagggtgta aatatcgttg gagttgttac agatcctgtt gatgataacg gtgaaaacaa
     1021 ggaagcaatt gaaaagtcaa agttaataca tgaaaagaca aaggcttcat atcctttctt
     1081 aatgccag
//
LOCUS       DAPCMT010000286         1088 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPCMT010000286 DAPCMT010000000
VERSION     DAPCMT010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.298"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(61..279)
                     /locus_tag="RD743_002916"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="RD743_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="RD743_002917"
     CDS             complement(545..739)
                     /locus_tag="RD743_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564674.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="RD743_002918"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="RD743_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          378 a          186 c          157 g          367 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgatta
//
LOCUS       DAPCMT010000287         1063 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPCMT010000287 DAPCMT010000000
VERSION     DAPCMT010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.299"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(38..994)
                     /locus_tag="RD743_002919"
     CDS             complement(38..994)
                     /locus_tag="RD743_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564675.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caa
//
LOCUS       DAPCMT010000288         1060 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPCMT010000288 DAPCMT010000000
VERSION     DAPCMT010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.300"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            77..192
                     /gene="rrf"
                     /locus_tag="RD743_002920"
     rRNA            77..192
                     /gene="rrf"
                     /locus_tag="RD743_002920"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            202..275
                     /locus_tag="RD743_002921"
     tRNA            202..275
                     /locus_tag="RD743_002921"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:236..238,aa:Asn,seq:gtt)
     gene            315..404
                     /locus_tag="RD743_002922"
     tRNA            315..404
                     /locus_tag="RD743_002922"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Ser,seq:gga)
     gene            419..490
                     /locus_tag="RD743_002923"
     tRNA            419..490
                     /locus_tag="RD743_002923"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Glu,seq:ttc)
     gene            506..578
                     /locus_tag="RD743_002924"
     tRNA            506..578
                     /locus_tag="RD743_002924"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:539..541,aa:Val,seq:tac)
     gene            588..660
                     /locus_tag="RD743_002925"
     tRNA            588..660
                     /locus_tag="RD743_002925"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:621..623,aa:Asp,seq:gtc)
     gene            696..771
                     /locus_tag="RD743_002926"
     tRNA            696..771
                     /locus_tag="RD743_002926"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:729..731,aa:Phe,seq:gaa)
     gene            773..853
                     /locus_tag="RD743_002927"
     tRNA            773..853
                     /locus_tag="RD743_002927"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:807..809,aa:Tyr,seq:gta)
     gene            860..933
                     /locus_tag="RD743_002928"
     tRNA            860..933
                     /locus_tag="RD743_002928"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:892..894,aa:Trp,seq:cca)
     gene            936..1008
                     /locus_tag="RD743_002929"
     tRNA            936..1008
                     /locus_tag="RD743_002929"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:969..971,aa:His,seq:gtg)
BASE COUNT          250 a          238 c          286 g          286 t
ORIGIN      
        1 ctcggaaaaa aacttatgtg acttcaaatc cagttttgag tgaacaaaat ttactcaata
       61 aaagataaca ccacagtgtg gtggcgatag cgagaaggat acacctgtaa ccatgccgaa
      121 cacagaagtt aagcttctta gcgccgattg tagtgagggg ttgccccttg tgagagtagg
      181 acgtcgccac gctgatgtta ttccggcata gctcagttgg tagtagcgca tgactgttaa
      241 tcatgatgtc gtaggttcga gtcctactgc cggagttctc attttgagaa agagtattga
      301 gcttctttca ctctggagag ttgtccgaga ggccgaagga gcatgattgg aaatcatgta
      361 gatggtgcca aactgtctca agggttcgaa tcccttactc tccgtagcaa atttacctgg
      421 cccgttggtc aagcggttaa gacaccgccc tttcacggcg gtaacacggg ttcgaatccc
      481 gtacgggtca ttttaattga atgttggagg tttagctcag ctgggagagc atctgcctta
      541 caagcagagg gtcagcggtt cgatcccgtt aacctccata atgaactggt tccgtggtgt
      601 aggggttaac atgcctgcct gtcacgcagg agatcgcggg ttcgattccc gtcggaaccg
      661 tcgttcttgt tattcaatta ataattttaa aatatggcgc ggtagctcag ttggtagagc
      721 aacggattga agctccgtgt gtcggcagtt cgattctgtc ccgcgccacc atggaggagt
      781 agcgaagtgg ctaaacgcga cggactgtaa atccgttcct tagggttcag tggttcgaat
      841 ccactctcct ccattttata ggggcatagt ttaaaggtag aacagcggtc tccaaaaccg
      901 ttagtgtggg ttcaattcct gctgcccctg ccatggcgaa tgtggcgaag tggttaacgc
      961 accggattgt ggctccggca ttcgtgggtt cgattcccat cattcgccct ttttattctt
     1021 ggggtatagc caagcggtaa ggcaagggac tttgactccc
//
LOCUS       DAPCMT010000289         1051 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.301, whole genome shotgun sequence.
ACCESSION   DAPCMT010000289 DAPCMT010000000
VERSION     DAPCMT010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.301"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="RD743_002930"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="RD743_002930"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDU0564676.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAPCMT010000290         1050 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.302, whole genome shotgun sequence.
ACCESSION   DAPCMT010000290 DAPCMT010000000
VERSION     DAPCMT010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.302"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            15..488
                     /locus_tag="RD743_002931"
     CDS             15..488
                     /locus_tag="RD743_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDU0564677.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            497..724
                     /locus_tag="RD743_002932"
     CDS             497..724
                     /locus_tag="RD743_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0564678.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          336 a          167 c          186 g          361 t
ORIGIN      
        1 aaagagggat aaaaatgaaa tttaaaacat tgctattaaa atttgtcgtt ttaggtataa
       61 gtgccgtatt tattttgttc ggcgtacttt tttatactca gatcgctaca agcgaaaaag
      121 gattcaattt tgattggcag accacattat ttgtttctgt catttacttt gcccttctcc
      181 tagggatcgt tattgcttat ttcttattga aactattgaa aaaaatcgaa gataaccaag
      241 ctttttccac tcaaagctta aacatactaa aaaaaatcga acgaaccatt ttgtttattg
      301 gtttcgcctt ttttggctta cttcctaaat ttatgaaagt agcggattta gacgatgctc
      361 caggtttgat gttggtagga atcgctgtcg tattcattcc ttttgcagta tacacattaa
      421 caagcgtgct ggaaaaactg cttgaacatg cgattttact taagacagat catgatttga
      481 cagtgtgagg tgcgacatgg caattatcgt taatttagat gtagtattag ctcaaaaaaa
      541 aatgagtgtg actgaattga gtgagaaagt gggcattacg atggcaaaca tctctattct
      601 aaaaaatgga aaagcaaaag ccatccgttt ttccactctc gaaaaaatct gccaagtttt
      661 ggagtgtcaa ccaggtgatt tgttgaccta taaaaatgac tcgaatacta taccagaaga
      721 ataaaataaa gtagaacaaa cagtaaataa aaaataatct gaatgataga tgtgtgagac
      781 ttttagaagt tatttacgtc tattattcag attttttatt tcgattgaat accgttttat
      841 ctataaataa gatatgtact gactcataca aagggggcct tttcttctcc atgtactatc
      901 acttttcctg atacaactgg tttcctgtta cgttgtagtt ggatctgctt ttagtataat
      961 actttattca ttccaccaga ctgatagcag tttgagcagt ctaaataatc atgtagtatt
     1021 acaggccatt ctgtttcata ccttgaagtg
//
LOCUS       DAPCMT010000291         1047 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPCMT010000291 DAPCMT010000000
VERSION     DAPCMT010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.303"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..572)
                     /locus_tag="RD743_002933"
     CDS             complement(<1..572)
                     /locus_tag="RD743_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDU0564679.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKP"
BASE COUNT          307 a          219 c          186 g          335 t
ORIGIN      
        1 ggctttacac tcaatagact gccaagagta gcacttgctt tggaaatcct accgcctcgt
       61 tctagatatt tcagatcgtc tactgttacc catgaatgga ctttcaaata attactttcc
      121 agccactcta gggtttcttc gatgctttta ccttcttttt tcaatcttac agcttccaat
      181 actaaaagcc cttccccaat acttgcagct tttgtatcaa aaacatagat ctcagggtct
      241 tcatattctt ccttcagcaa ttcaaccgct tgaagcgcac tactgtaaga gccgcttaat
      301 ccactggaaa aagccacata taaaacagga atctgttttt ccgcataaga acggaaaaat
      361 tctaaatatc ggccaacatt gatttgtgat gtcgtaggca tcgcaccatt tttcagctga
      421 ttcatgtagc ggtcataatt gaatgtcttt ccgaggtcat ctactaactc ttgatcattc
      481 aattggagat tcattgataa aaaatcaact ttttcttttg ctaatacatg gtatggcaga
      541 tcacaacaag aatctgttac aatttgatac acatttttca accccttctc gttacatttt
      601 acccttatcg tctattatag caaatctctt gcgctagaca cactttaacc ggtttatttt
      661 cgcaagttct aatcaaaata agaggttgtg acagaagtgg tcagcttcaa gaaataagaa
      721 ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt cggcttattt ccgtagaagc
      781 tacttctgga acaccgtgaa tcagaggttg tgaaatcagc gttttgacct gagtattaga
      841 gaaaaaaacg gaaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
      901 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttagct ccgagtcaca
      961 aggaacaatt tcaaaaaaca gcttctcatg tttgttgaat attgtgactt gtgaccgagg
     1021 agttggcagg agaacaccgt ttagtgg
//
LOCUS       DAPCMT010000292         1038 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.304, whole genome shotgun sequence.
ACCESSION   DAPCMT010000292 DAPCMT010000000
VERSION     DAPCMT010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.304"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            98..838
                     /locus_tag="RD743_002934"
     CDS             98..838
                     /locus_tag="RD743_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564680.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          382 a          184 c          200 g          272 t
ORIGIN      
        1 atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat ctgtgctatt
       61 attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca
      121 tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg
      181 agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc cgtttgaaca
      241 gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc
      301 aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt
      361 accggattat accggccatt accaatcaga aatcacatcg acaggacgaa aaaaactttt
      421 tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca agcatactac
      481 acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt
      541 acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc atttaatcga
      601 atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga cgccgtatca
      661 agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa cgttagagga
      721 aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt
      781 acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt ttaaataagt
      841 gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt ttccttattt
      901 tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta agcgaatttt
      961 ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt tcgcagatcg
     1021 tttgcaaaaa tagtttat
//
LOCUS       DAPCMT010000293         1027 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPCMT010000293 DAPCMT010000000
VERSION     DAPCMT010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.305"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            198..>1027
                     /locus_tag="RD743_002935"
     CDS             198..>1027
                     /locus_tag="RD743_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDU0564681.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          362 a          149 c          175 g          341 t
ORIGIN      
        1 taagagtttg gacctagcaa acaatattaa tgaggtcatt aaatcttatt atctttagtg
       61 atataacgaa atatctacac agacaaaatg atagcgtaaa atattttgtg taaatagata
      121 tttagggtag aaaaaaagaa agtccattct gtaaaattaa agttaccaca accgaaattt
      181 tataggagga ctttctcatg cccaatttta ctacagaaat tatggaaaca ctaatcaata
      241 aaggtgattt agatgaatta tttcgatgtc atttagaatt agctgttaat tcattattgc
      301 aggctgaatt aacagctttt cttgattatg aaaaatatga tcgtgctggg ttcaattcag
      361 gtaattcccg taatgggaat tattcacgtt cattcagaac agagtatgga gaattaaatt
      421 tggtaattcc tagagataga aatggtaagt tttctcaaca aactttacct gcttataaaa
      481 gaaccaatga ctctttagaa accactatta ttcagctatt tcaaaaagga attactatgt
      541 ctgaaatctc tgaattgatt gaaaaaatgt acgggcatca ctatacgcca caaaccattt
      601 caaatatgac taaaatagtt tctgaagata ttattgcctt taaagaaagg tccttagaat
      661 ccagatactc tgtcattttt atggatgcta ctcacattcc tttaaaaaga caaactgttt
      721 caaaagaagc agtttatatt gtaattggca ttcgattaga tggaaccaaa gaggttcttg
      781 ggtttagtct tgctccaact gaatctgctt atgtttggaa agagattctt caagacctaa
      841 aagaccgtgg tttaaaagag gttttattag tcgtaactga tggtttaagt ggcattaacg
      901 atagtattca tagtgtttat ccaaatgctc aatttcaaca atgttgtgtc catatatcta
      961 gaaatatcgc tcataaggtt cgtgttagtg atcgacaaga aatctgtagc gatttcaaat
     1021 tagttta
//
LOCUS       DAPCMT010000294         1020 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPCMT010000294 DAPCMT010000000
VERSION     DAPCMT010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.306"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            50..583
                     /locus_tag="RD743_002936"
     CDS             50..583
                     /locus_tag="RD743_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDU0564682.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            599..769
                     /locus_tag="RD743_002937"
     CDS             599..769
                     /locus_tag="RD743_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564683.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
BASE COUNT          419 a          178 c          176 g          247 t
ORIGIN      
        1 tatctgattt agataattta ttccagtagc aacctaggag gcaaccagta tgcaaccaca
       61 agaaaccttt acaataaagg aattgtcaga actttttaag atgagtaggc aagcaatatc
      121 gaaacatatc caaaaattag attcttccat gatagctaaa aatgagcgtg gttataaggt
      181 tgtattgcgt tctggagtat tgcaacttgc tcgcaactta ggcaaccaaa tgttgctaga
      241 taggttgcac gagtacgaac aagacaacga taaaaatgtt aatataccaa gcaaaaacgt
      301 ggttacaaac aagttgctag atcagttgcg tgaagaaaat gaatttttac gagaacaact
      361 tgccctcaga gagcaaaata attctcatat acaaaaacta ctcgaccagc aacaacaact
      421 caccctacaa gccaacaaac aaatcgaaaa actgcaagaa aaactacagt tgacttatac
      481 agaagaagct ccagagaatg actcaacgac tctttctgaa gcaacagaaa caatagacaa
      541 acaaattgat actgaaaaaa aatggtggca actttggaaa taactggagg gaacattcat
      601 gacaagaaaa aatcttctat tcggttctat tttactagga attctaggat tcagtccgat
      661 gttattgtca gtgatcaata aagggaataa acatggtgaa ttgaaattga atccaaatta
      721 ttacaacagt cctaacgaag aattgtttgt aaaagaaaca gataaataat aaaaaagact
      781 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      841 tatgaagcgc taccaaaaat ttatcatatt ttattaaaaa taacaaccca gtaaaaaatg
      901 actggtaaaa attcacaaag aaacggaaag aagcaaccgc agaaaatcaa gcttaaaaag
      961 agcttttgac ctagaccaat cgaaaatcac aaagtgagcg cctgtatcta ttgctacagg
//
LOCUS       DAPCMT010000295          997 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPCMT010000295 DAPCMT010000000
VERSION     DAPCMT010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.307"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            162..395
                     /locus_tag="RD743_002938"
     CDS             162..395
                     /locus_tag="RD743_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDU0564684.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            392..799
                     /locus_tag="RD743_002939"
     CDS             392..799
                     /locus_tag="RD743_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDU0564685.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          375 a          134 c          171 g          317 t
ORIGIN      
        1 gaaggaaata gaagggagca atctaaattt tttgaatttt cttaattaat tatatagaaa
       61 gaacaatctt tctatagagt tagacatgag agggactatg cttgatttaa aatcaacaag
      121 cgtatatact gtatatacaa agaaaaagga gggagaagac tatggcaatt aaagaaagaa
      181 aatttaggga agtggggaac tcagtagtat taactatttc aaaggagttt cttaaagaaa
      241 atgggttaaa tcccggagat acggtttttt tagatgaaga aaaacttcga gcggcaatta
      301 caaaaaaaca agccttatct gatgaaattg atttagcaat atctcaagca ttaactgaat
      361 atgatactac ttttagagag cttgtcgata aatgagtatt cattacctag aagccaaaga
      421 tattataaaa atgaatgtaa tccaaataaa aaaatactct cctaatgaac cccttggtgt
      481 aaaggatgcg aatgctttag aaatgtgtgt agggcaactt cgagcctcag cttttggtga
      541 agaggtttat ccttcgattt atgaaaaagc agctatccta cttatacaat tgataaagaa
      601 acatcctttt cacaatgcaa ataaacgtac tgctttctta gctacttttg tctttttgaa
      661 aatcaatgga cagcttttag ctattgatca aaaagatgct gtaaatttag cggtatatat
      721 tgctacgtat gataaggatt tcgatcaact aaaatatgaa gtgatcaaca ctataaagac
      781 acatacgcag cccatttaag gagttactgt atgtgtcttt atagggttat tagctgctat
      841 agcgaaataa ttgaagtaaa ttacaaatag ctataattca aatccaacta tatagaaaac
      901 gtaaattttc tatatagttg gattaagata acgactattt attttatcat atttttacta
      961 taccctatac ttttacttgg tatatttatg aataatc
//
LOCUS       DAPCMT010000296          997 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPCMT010000296 DAPCMT010000000
VERSION     DAPCMT010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.308"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(26..913)
                     /locus_tag="RD743_002940"
     CDS             complement(26..913)
                     /locus_tag="RD743_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDU0564686.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DAPCMT010000297          994 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.309, whole genome shotgun sequence.
ACCESSION   DAPCMT010000297 DAPCMT010000000
VERSION     DAPCMT010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.309"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..>994)
                     /locus_tag="RD743_002941"
     rRNA            complement(<1..>994)
                     /locus_tag="RD743_002941"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAPCMT010000298          992 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.310, whole genome shotgun sequence.
ACCESSION   DAPCMT010000298 DAPCMT010000000
VERSION     DAPCMT010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.310"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            359..992
                     /locus_tag="RD743_002942"
                     /pseudo
     CDS             359..992
                     /locus_tag="RD743_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          339 a          181 c          182 g          290 t
ORIGIN      
        1 agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa caccttcaat
       61 tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt aaacgaatta
      121 ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag tgcaaatatt
      181 aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga tctatatatt
      241 catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata taggctctat
      301 aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt ttaggtatat
      361 gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa ggaccctgcc
      421 aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata aaatgagctt
      481 tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac gaaaattgtc
      541 tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat atttagtgac
      601 agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg cgaaagtcgt
      661 cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt ttcgtgtaca
      721 agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag tgaaaaagaa
      781 ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca aaacatggcg
      841 cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg atcgattgct
      901 ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga ccgaagcctt
      961 tagagacaag gaatcagact tattttttac ct
//
LOCUS       DAPCMT010000299          976 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPCMT010000299 DAPCMT010000000
VERSION     DAPCMT010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.311"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            82..>976
                     /locus_tag="RD743_002943"
     CDS             82..>976
                     /locus_tag="RD743_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDU0564687.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHY"
BASE COUNT          331 a          182 c          192 g          271 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatg
//
LOCUS       DAPCMT010000300          972 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPCMT010000300 DAPCMT010000000
VERSION     DAPCMT010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.312"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..834)
                     /locus_tag="RD743_002944"
     CDS             complement(<1..834)
                     /locus_tag="RD743_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0564688.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HM"
BASE COUNT          292 a          188 c          149 g          343 t
ORIGIN      
        1 catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa aagctagtgc
       61 atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg cgttcttcgc
      121 taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac aaatatcgat
      181 tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa aaaaaacacc
      241 acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa tcggatacgt
      301 ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact ctttttctct
      361 agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt tggctgtttc
      421 tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat tcgtaaaaat
      481 catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt caattacctt
      541 gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa tcagtgaaaa
      601 ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg aagaaaaaag
      661 ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta acgaagggac
      721 ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt cagacacttc
      781 tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt tcattatttt
      841 ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat gttttctttg
      901 ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct tacggtatct
      961 atgatttaag ct
//
LOCUS       DAPCMT010000301          971 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPCMT010000301 DAPCMT010000000
VERSION     DAPCMT010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.313"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..880
                     /locus_tag="RD743_002945"
     CDS             <1..880
                     /locus_tag="RD743_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDU0564689.1"
                     /translation="FKYLIEVNKLLKKESFSIVHNQVYFGGGINLWLAKKNGIRQRIA
                     HSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGID
                     LELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYL
                     ILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGL
                     EAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYD
                     VSYTTEQMESIYSRDH"
BASE COUNT          376 a          107 c          184 g          304 t
ORIGIN      
        1 ttttaaatat ttaatagaag tgaataagtt actaaaaaaa gaatcttttt ctatagttca
       61 caatcaggtt tattttggcg ggggaattaa tttatggtta gccaaaaaga atggaatcag
      121 acagagaatt gctcatagcc atgcaactga agatggaaaa tctcaaaata ttgtaatgaa
      181 tgtattgcgt aaatttttaa ccaaattgtt gttacaaaat gcaacagact accttgccgt
      241 tagtcaagaa gctggtgaaa gtctgtttca aaaccatccc tttgagatag ttcataatgg
      301 tattgattta gagctttata gtaaaaattc agaagctaag gttaataaga gaaaagaatt
      361 ggatatctca atgagtactt ttgttgtggg aaacattgga cgattagaga aacaaaaaaa
      421 tcaatcttat ttgttagaga tttttttaga aatactaaaa atgaattctg attcttactt
      481 gattttagta ggtgaagggt cacttagaaa taaactgaaa gccaaagcag tagaattgga
      541 tatagagaaa agagtaaaat ttttaggaga aagaaacgat attcccgagt tattatctgt
      601 gatggatgtt tttgtaatgc cttctcttta tgaaggtctg ccaatggttg gattagaagc
      661 acaagcagca aaattaaaat tagtcttagc agatactatt tccaaagata caaagttaac
      721 aaataatgtg gattttgtat cattagatga aacaccacaa gaatgggcaa aaaaagtaat
      781 aaaagagccg tatgctaata aaataacaca agaactaaag agttatgatg tttcgtacac
      841 tactgaacaa atggagagta tatatagtag agatcattaa aaatgttcaa gatattttag
      901 gagtgattat ttttgaaaat atgttatttg atattggcat acaatgatat tggcaactta
      961 aagagattgg t
//
LOCUS       DAPCMT010000302          959 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.314, whole genome shotgun sequence.
ACCESSION   DAPCMT010000302 DAPCMT010000000
VERSION     DAPCMT010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.314"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(107..511)
                     /locus_tag="RD743_002946"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="RD743_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="RD743_002947"
     CDS             complement(570..>959)
                     /locus_tag="RD743_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564690.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAPCMT010000303          954 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPCMT010000303 DAPCMT010000000
VERSION     DAPCMT010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.315"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..>954)
                     /locus_tag="RD743_002948"
     CDS             complement(<1..>954)
                     /locus_tag="RD743_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDU0564691.1"
                     /translation="DNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSPS
                     YCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEH
                     HQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVLC
                     MDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLVM
                     DMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRR
                     LKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLS"
BASE COUNT          268 a          186 c          196 g          304 t
ORIGIN      
        1 agcttaacag ctcatcaacg atttctgttt gactaattgg tcgtttaaac agtcgatgat
       61 aatgccgttt gagtggctcc catgtcgtgg aatctttgag taagagtttc caatatcgct
      121 tgagccgacg gtacttcttc tgattttcag agtgtgaggt tcgaaaacga ttcatgatct
      181 ggacacgtaa ttgattgaag ctgcgattga tgtgttggac aatatgaaag cggtcagtta
      241 cgatctccgc atttggaaag actgttttaa gcaactgaca atagctggca ttcatgtcca
      301 tcactaggta cttgaccttt aaacgggctt ttctaggaaa gtgcaggaag tattgagtta
      361 gtttgtaaag tctgcgatct tcaaggatcg taaatagctg atgggttgtc ccatcaacac
      421 aaataaaact catggaacca gaaacagact tcattgattt gaactcatcc atacacaaca
      481 ccgtcggaag gaagttcata cgtgactggt aatgcttcgt tgcttcacgc aagatccgtg
      541 tcaccgtccc atccgagacg aagtacttct tagcgattag tttacgtgat tgattttcgt
      601 acagttccat gagaattgcc tgcttcagtt cctttgttag ttgatgatgt tcatcaacta
      661 agtccgtttt cgcagaaaag gtccttccac aatcatggca gagaaaacgg gtacgtttta
      721 attctaaaat agttaacttt tctttgactt tcaatatctg agtgcgtgtc gtataggaac
      781 cgttgcggat aatacgaacc gatcgacagt gttcacaata ggaaggagag tcatccaatt
      841 ttcctttaat aaactgcgct tgaacgcctt tatattcttt ttcaatcagc cattcttcat
      901 caaaaaataa atgtttatct gttaatccga gtaattttct agtatgatta tcca
//
LOCUS       DAPCMT010000304          951 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.316, whole genome shotgun sequence.
ACCESSION   DAPCMT010000304 DAPCMT010000000
VERSION     DAPCMT010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.316"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..162)
                     /locus_tag="RD743_002949"
     CDS             complement(<1..162)
                     /locus_tag="RD743_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDU0564692.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEY"
     gene            371..583
                     /locus_tag="RD743_002950"
     CDS             371..583
                     /locus_tag="RD743_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564693.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            745..>951
                     /locus_tag="RD743_002951"
     CDS             745..>951
                     /locus_tag="RD743_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDU0564694.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYR"
BASE COUNT          331 a          156 c          156 g          308 t
ORIGIN      
        1 atattcttgc acccaacgat aaatcgtcgt atgagaaacg ttaatgccac gatcatataa
       61 gatttcttga acttcacgat agctaaggtt ataacgaaga tagtagccca cggctacaat
      121 aatcacatcc tgctgaaatt gctttccttt aaaatgattc atcgtcattc ctcctgctat
      181 ctttttctat tattctacct tatttgatag tagatttaaa actttgcaac agaaccctaa
      241 aaacactata tattccgaaa agtgagtatc tccattcata aaataatcaa catatttaaa
      301 aaatctaatt agctttttta gtcaatctaa agttctttat tctatgatat aatgttactg
      361 tagaaagttt gtggcggttt ggcaaatcca attgaaggag cgtggtgcct atgggcttat
      421 tagcttatcg gacgtactct gtagaaagga aatgcctgat ttgttctcat tggatcaaat
      481 cattgctatt atcggagcag cagcagtagt tattactgcc gtagcgaaag ctagcgattc
      541 gattttagct tggttaaagt tcaaaaaaga acaaaaaaaa tagagccgtc tcaacttttg
      601 gcgaggtaga cgagctctaa agctttattt acatgccatc cgctttgaac ggactacact
      661 gggcatgtga cagcatgccc tttcatttat attatatctc attcttttat ataatgaaag
      721 taaataaatc atggaggata aaatatggat aaatttaata gattaacatt aataaatcag
      781 tttgaaattt taaaagcttt aaatcctaac gaagaaaaat actatgctga aaaaatagaa
      841 atactaacag aaggttacga atatcactat agtgaaatat ttgaaaatat ttctgatcca
      901 ttgccagaag aagattcgcg gtttgttcta gacatattaa atatgtatag a
//
LOCUS       DAPCMT010000305          931 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPCMT010000305 DAPCMT010000000
VERSION     DAPCMT010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.317"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>931
                     /locus_tag="RD743_002952"
     CDS             <1..>931
                     /locus_tag="RD743_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDU0564695.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAPCMT010000306          916 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.318, whole genome shotgun sequence.
ACCESSION   DAPCMT010000306 DAPCMT010000000
VERSION     DAPCMT010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.318"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..521
                     /locus_tag="RD743_002953"
     CDS             <1..521
                     /locus_tag="RD743_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564696.1"
                     /translation="FTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREIS
                     LKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICG
                     KTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKL
                     NQKTIKKLNIGG"
     gene            524..>916
                     /locus_tag="RD743_002954"
     CDS             524..>916
                     /locus_tag="RD743_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564697.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIREL"
BASE COUNT          350 a          157 c          176 g          233 t
ORIGIN      
        1 gtttcaccgt acagcaacga aacttcatct atgcgctgat aggcgatatt tttatcgata
       61 caggcatgcc aacggacttc tggaaggaat tcttctactt ccgttttgaa ggtgtcacag
      121 ggcgcgaaat aagcctcaaa gacgaatcga atacaaccgt gagtgatgcc aatatcttag
      181 cgaatatcat cctagatttc atctttgaac atcatattcc tttcaaagaa ggatatgaga
      241 ttttaccagc gaatcaagag tattacttct acaaatgcat tacaaaaaga gtctgctgca
      301 tctgtggcaa aacaggagct gacatcgatc actttgacaa agcgctagga agacgaaagc
      361 gcaaagaagt tgatcatgca gagtacacat ttgcagcact ctgcagaatc catcacacag
      421 agaagcacaa aataggtgtg attaatttca aaaataaata tcaaatcaaa gggatcaaat
      481 taaaccagaa aacaatcaaa aagttaaata ttggagggta aaaatgacag aacatcgaag
      541 ttattatgcg attataccag ccaacgtaag gtacgacaaa agacttaaac caaatactaa
      601 gttgttatac ggagagataa cggccttgtg taatgaaaga ggcttttgtt gggcaggcaa
      661 tgagtacttt gcagatttat atggtgtgaa taaagagacc atatcgcgat gggtaagtga
      721 tttgattaag tttggatact tgaatcggga aatcatttac aaagagggta ccaatcaaat
      781 aatcaatagg tacctacgaa ttaatcaata ccctattgac gaaaaacgca atacccctat
      841 tgacgaaaaa gtcaaagata ataatacatc tattaataat acatttaata atacaaaaga
      901 atatataaga gagtta
//
LOCUS       DAPCMT010000307          892 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPCMT010000307 DAPCMT010000000
VERSION     DAPCMT010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.319"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            63..>892
                     /locus_tag="RD743_002955"
     CDS             63..>892
                     /locus_tag="RD743_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HDU0564698.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          309 a          134 c          163 g          286 t
ORIGIN      
        1 aaggaagtcc cttctgtaga atagagttac cacaacacat tcacagaaaa gaggacttcc
       61 atatgaacga ttttactaca gaaattatgg aaatactaat caataaaggt gatttggatg
      121 atttgtttcg tcgtcaccta gaattggcta ttaacgcttt attacaagct gaattaacgg
      181 tatttcttga ctatgaaaag tatgatagag ctggatttaa ttcaggtaat tcccgtaatg
      241 ggaattattc gcgctctttt aaaacagagt acggtgaatt gaatttagta atacctagag
      301 ataggaatgg agacttttct caacaaactt tacctgctta taaaagaacc aatgactctt
      361 tagaaaccac tattattcag ctagttcaaa aaggaattac tatgtccgaa atctctgatc
      421 taatcgaaaa aatgtatggt cattattaca aaccacaaac tatttcaaat atgagtaaaa
      481 ttgtgtctga agatgtttcg gcatttaaag aaagaacttt agaagctaaa tactcagtca
      541 tttttatgga tgctactcat attcccttaa aaagacaaac cgtatcaaaa gaagctgtct
      601 atatagtaat tggtatcagg ttggatggaa ctaaagaggt tctaggattt actatagctc
      661 caactgaatc tgcttatgtt tggaaagaga ttcttcaaga tctaaaagac cgtggtttag
      721 aagaggtttt gttagtcgta acagatggtt taagtggtat tgacgatagt attcatagtg
      781 tttatccaaa tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata
      841 aggttcgtgt tagtgatcga caagaaatct gtagcgattt caaattagtt ta
//
LOCUS       DAPCMT010000308          890 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.320, whole genome shotgun sequence.
ACCESSION   DAPCMT010000308 DAPCMT010000000
VERSION     DAPCMT010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.320"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..888
                     /locus_tag="RD743_002956"
     CDS             <1..888
                     /locus_tag="RD743_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDU0564699.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAPCMT010000309          885 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPCMT010000309 DAPCMT010000000
VERSION     DAPCMT010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.321"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(243..464)
                     /locus_tag="RD743_002957"
     CDS             complement(243..464)
                     /locus_tag="RD743_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564700.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="RD743_002958"
     CDS             complement(562..807)
                     /locus_tag="RD743_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564701.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          266 a          173 c          113 g          333 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccat
//
LOCUS       DAPCMT010000310          885 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPCMT010000310 DAPCMT010000000
VERSION     DAPCMT010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.322"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            87..356
                     /locus_tag="RD743_002959"
     CDS             87..356
                     /locus_tag="RD743_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDU0564702.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="RD743_002960"
     CDS             349..606
                     /locus_tag="RD743_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDU0564703.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          337 a           97 c          168 g          283 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat taatgaatgt
      721 gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt tgaggaaaag
      781 taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt ataatactat
      841 tgtagtgtgg gatgttagtt actaaaggat gacgcttata tatat
//
LOCUS       DAPCMT010000311          844 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPCMT010000311 DAPCMT010000000
VERSION     DAPCMT010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.323"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            86..700
                     /locus_tag="RD743_002961"
                     /pseudo
     CDS             86..700
                     /locus_tag="RD743_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          312 a          133 c          150 g          249 t
ORIGIN      
        1 gacaaaaata taacattgaa aaatttagag ttctaacgta tgattagggt gtattataaa
       61 cgttatagaa aagtagggaa taaatttgaa aattgcttat attagagttt catccatcga
      121 tcaaaatgaa caacgtcaaa ttgaagaaat gaaaaagttt ggcgctgaaa aaatttttat
      181 cgaaaaacaa tctggagcga cgattagtca tcggccgatt ttccaggaag ctttagattt
      241 tgtcagagag caagatattt ttattgtaga agccattgat cgacttggac gcaattatga
      301 cgagattatc gattcagtga attatctaaa gaagaagaat gtgcgactta ttatcaccag
      361 tttaccgatc atggctgaag caattggaaa tcctctccta gataaattca tcaaagactt
      421 aatcatttaa atctcagcca tgattgctga acaagaacgt actgaatcga aacgacgcca
      481 ggcacaagga attaaaatcg ctaaagcaaa cggcgtctat aaaggtcgtc ctaagttata
      541 cagtgcagac accaaagatc ctcaacgtcg ccttgtctat aggagtatcg ttcaggatct
      601 tgagaatggg gttgctatct caaagattgc aacagactac aatgtgacta ggcaaacaat
      661 ttaccgaata aaaaaagaaa tcgaccaact aattgtatga tttctttttt gaatcacgta
      721 attattaatt agctttgtca tcaatacaga caatcatttt agattcatct agctaagttg
      781 attacattta tgttaaaaag aatttagtta acataaatat aattgatgca taaatcgtcc
      841 ttat
//
LOCUS       DAPCMT010000312          825 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPCMT010000312 DAPCMT010000000
VERSION     DAPCMT010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.324"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..153
                     /locus_tag="RD743_002962"
     CDS             <1..153
                     /locus_tag="RD743_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDU0564704.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKV
                     LMGITA"
     gene            215..>825
                     /locus_tag="RD743_002963"
     CDS             215..>825
                     /locus_tag="RD743_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564705.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMK"
BASE COUNT          294 a          122 c          163 g          246 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcggcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgag aaccttgggt tttcaagtgc tttttggttc
      241 attaatctta accatagtgt ttttaaagtg gaaaacaagc aacaaaaaaa gtatgtggag
      301 ccttgtagga atagttctta ttatttttac tgtgactgca gcaacagaag caaattattc
      361 gcataaaatt cagtcgtata aaggtcgttt agttatgacc aaaaaagatg ttacacgtta
      421 tacatcctca agaactggag gcctatatga ctatgcacca ttagatgcat tgaaatataa
      481 agatcattta aagaaacatg aagtaaaagt tggtgatcgg tgggagaatg catctcataa
      541 agcatataat tccaaaatag tctatacagt agaagatgcc gatgagcaaa atatcacatt
      601 acctgtattc aattattggg gaacagttgt gaaagtaaat gggaaaaaga gcactataga
      661 aaatgacaat ggtttagtgt ctattaaagg tacggggaaa agaactatag ttgaggtttc
      721 atctacgtat ccaactacga tgattctatc tttattaatt agttcaataa cttttatatt
      781 cttatttaca cgttatttaa taagatgctg cggtgaaatg aaaat
//
LOCUS       DAPCMT010000313          821 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPCMT010000313 DAPCMT010000000
VERSION     DAPCMT010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.325"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..805)
                     /locus_tag="RD743_002964"
     CDS             complement(<1..805)
                     /locus_tag="RD743_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0564706.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYT"
BASE COUNT          271 a           98 c          146 g          306 t
ORIGIN      
        1 ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt tttagttctt
       61 taaaatagct aataacactt tttccattca cttttctaaa atgttttttt aagctggctg
      121 gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa agatgttctt
      181 ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc ttttttttag
      241 gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga taatgccttg
      301 gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa agcaaatgaa
      361 tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag gaaatagctg
      421 gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa gtaatgatag
      481 atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat aattgctcat
      541 caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa aagatttgga
      601 cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata gatttatgtg
      661 caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag ggagcagcag
      721 ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga ttggaggatt
      781 ggttgtttgt taacaaaaga accactttcc tttctctttt t
//
LOCUS       DAPCMT010000314          819 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.326, whole genome shotgun sequence.
ACCESSION   DAPCMT010000314 DAPCMT010000000
VERSION     DAPCMT010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.326"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            180..812
                     /locus_tag="RD743_002965"
     CDS             180..812
                     /locus_tag="RD743_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDU0564707.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          286 a          117 c          132 g          284 t
ORIGIN      
        1 ttctaagatt tttattagaa ttacgcctaa tttgtgtctc tcagggcaat tctaagaacg
       61 tttcaagaga tagcgacact atcgttatcg aaaaaggcga aaacctatct cattttctgc
      121 taatgtaagt tggtttagct tttgttaata tgactttatg aaaatggagg taattaaata
      181 tgatttttcc taaagttaat gaagcaagct tgaataaagg atcctttttt catatctatg
      241 aaaacgaaga ttttgaactg tataatatcc tttcacaaaa agaggtcatc gaaaaccaaa
      301 taatctgcca tccaattatt tacaaatcta ttttcaagta tgcatttttt gcttccaatg
      361 attttcaaag aatggagatt accgactgta tattcgaaaa ctgtgatttt tcaggctgta
      421 tattgacaaa aagcatgttg catcgcgtga aatttgtcaa ttgtaaatta acaggtacta
      481 aattgatgga aagttatatt gggaacacgt tattcgagaa ttgtaaaatg gactatgtaa
      541 atttatctgg ttcaaacata aaagaaagta cctttgagca ctctgtctta tcaagcgcag
      601 attttgtaga ctgttccctc actaaaataa tgttttctac taatgattta gaaaatgcta
      661 gcttttttga tactgattta aaaaatatcg atttaagtag caactccttt gaaatgattg
      721 aagtacgctc ggaacatcta aaaaattgta cgcttaatca gttgcaagca cttggttttg
      781 ctagaaaatt tttacaaata aaagtagtat aatcaaaat
//
LOCUS       DAPCMT010000315          808 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.327, whole genome shotgun sequence.
ACCESSION   DAPCMT010000315 DAPCMT010000000
VERSION     DAPCMT010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.327"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            358..>808
                     /locus_tag="RD743_002966"
     CDS             358..>808
                     /locus_tag="RD743_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDU0564708.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYY"
BASE COUNT          273 a          147 c          128 g          260 t
ORIGIN      
        1 accgaatgca aaacagaagt tctataagtt ataatcatag ttgttaggtg attcatctag
       61 taaaaacacc agatttcaaa tttcacacaa tttaaactag atcaatcatc aaccttcttc
      121 aaattgttag aaccctttcc cgaggagctg cagcataagc aaaaaaacaa acaggaagcc
      181 aaatggctaa gacatttttt ctattagtct agttgtttct cgtcgcaagt ttaagacttt
      241 agaaagttat ttctaatgat gtttgggttg ttttgatcaa tgcttatgca cagctcctta
      301 cctattattt atctcaacag atctaacaaa ttaatggact atttaaggag tttttttatg
      361 cttacgatct attcttctcg cacacatagt taccatctga tggcaaactg gctgaaagac
      421 catcaaatgc cttacagaga acgaattttc acacctaaac agcctttaac aaggaaagag
      481 atattttatt tattgtcatt aacagaaaac ggatttgacg atctattagc taaacgctcg
      541 attgattata aaaatttgaa agaccaaatc gaccaatgta cgacctcaga gcttgtcgat
      601 ctgatcgtct cgcattttac tttgttacaa cgtcccatca ttactgacgg aaagaaatta
      661 ttagtcggat tcaatgaaga tagcatgcgt ctttttatcc ctgtacaatg gcgcagaagt
      721 caactgaaat ctgtctataa cgattttaca tgggtaagca atctagaaaa ttctacggat
      781 ttagatgatg aatttttaaa ttattata
//
LOCUS       DAPCMT010000316          774 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPCMT010000316 DAPCMT010000000
VERSION     DAPCMT010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.328"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..704
                     /locus_tag="RD743_002967"
     CDS             <1..704
                     /locus_tag="RD743_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDU0564709.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          264 a          127 c          145 g          238 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atca
//
LOCUS       DAPCMT010000317          761 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPCMT010000317 DAPCMT010000000
VERSION     DAPCMT010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.329"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            51..731
                     /locus_tag="RD743_002968"
     CDS             51..731
                     /locus_tag="RD743_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDU0564710.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          270 a          127 c          152 g          212 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
      721 tcccagctta aatcatagat accgtaaggg attttattct t
//
LOCUS       DAPCMT010000318          748 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPCMT010000318 DAPCMT010000000
VERSION     DAPCMT010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.330"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..627
                     /locus_tag="RD743_002969"
     CDS             <1..627
                     /locus_tag="RD743_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDU0564711.1"
                     /translation="GDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTS
                     ITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLIS
                     SVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIML
                     KSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          263 a           88 c          139 g          258 t
ORIGIN      
        1 ggagatattc gaaatttaga aaacatggtt tttaatctga gtaatggaat aattccaagt
       61 gttagtggag atacgattcg gtctgaaaaa aattattcta tcattgtttt tgaaaagtta
      121 gcacaaacgt ctataacatt aggaatggat ataatagaag cgtatcaaag tagagatgct
      181 ttgatacaag aaaacgaatt agctgtatcg ttacctgagg tcttaaaagt gagggattct
      241 gggattgttt attatactaa ggaaatcgga aaaactaaga tagagcatct ttctccctta
      301 atttcttctg tcgttcaatt catcggctta aatatctata aaagaattac tgtaaaggaa
      361 atagctaatt atttttctgt aagtgaaact aaattaagag aaagctttaa gaatgaaatg
      421 catattacga tttataatta tatctctaag cgaaaaattt ctacggctaa aataatgttg
      481 aaatcaaacc atactatcag tgaagtttct ttaggtttag gattttcaga ttcttcacac
      541 ttttcgagag tattcaaaaa gtacgcaggt gtatctccta agcaatatca attaggttta
      601 gtggacaatt tcaacaacat tagctgagag gttttatttt attatgagac tatatcattt
      661 acgtagtttg gaggagttga ggaaattgga ttttaacaaa aaattttttt gggaaatttc
      721 tgatttgagt tgtttcgtag aatctgag
//
LOCUS       DAPCMT010000319          744 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPCMT010000319 DAPCMT010000000
VERSION     DAPCMT010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.331"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..106
                     /locus_tag="RD743_002970"
     CDS             <1..106
                     /locus_tag="RD743_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0564712.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="RD743_002971"
     CDS             complement(359..508)
                     /locus_tag="RD743_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564713.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          240 a          117 c          111 g          276 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataa
//
LOCUS       DAPCMT010000320          738 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.332, whole genome shotgun sequence.
ACCESSION   DAPCMT010000320 DAPCMT010000000
VERSION     DAPCMT010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.332"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..625)
                     /locus_tag="RD743_002972"
     CDS             complement(<1..625)
                     /locus_tag="RD743_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDU0564714.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
BASE COUNT          222 a          133 c           83 g          300 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaacta
//
LOCUS       DAPCMT010000321          731 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.333, whole genome shotgun sequence.
ACCESSION   DAPCMT010000321 DAPCMT010000000
VERSION     DAPCMT010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.333"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..329)
                     /locus_tag="RD743_002973"
     CDS             complement(<1..329)
                     /locus_tag="RD743_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564715.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLF"
     gene            516..728
                     /locus_tag="RD743_002974"
     CDS             516..728
                     /locus_tag="RD743_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564716.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
BASE COUNT          260 a          126 c           95 g          250 t
ORIGIN      
        1 ataaataatg aatcaggttt gtttataaat tccctaatgc ctgcagaatc tactattttg
       61 tatctttcta aaccttcata gtctttttgc ctcttaactg ttatttgtac aggatctgga
      121 tgccatacac tttcagaata ttcccaggta gtatcatcta taatttttaa attatgaata
      181 agaccaccta cttcttcttt ccaaacagtt cctggttgca ataaactatt atcttcttta
      241 gcttccgatt tcttattact tgaacatcca cttaaaataa taaaaaatat tgcaaacata
      301 gagacaaacc atagttgctt tttcttcatt ttattccttc tttcgttgtt tatttttagt
      361 atatcaaata acgaaagtag caacaaacct catttggata cacggagaac gaatactcat
      421 aaaaatccac ctttctatag aaaatttttc tcttgatatt gtgattcatt acaaaaaaga
      481 gctataataa agaaaaaaca agaaggagca ttgaaatgta cgctgataaa aagaaaatcg
      541 aatggttgct gtttgatagt ggtaaatcag ttcgttctat atccatagaa tctggcgtac
      601 ccttaactac agtaagtgac cttgccaaca aacgctctag tatagacaac atgagattca
      661 taaatgctat gaaattaaat aaattggctg aagaaatcgt tgatattaat acttcaacta
      721 acaaataaaa t
//
LOCUS       DAPCMT010000322          720 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPCMT010000322 DAPCMT010000000
VERSION     DAPCMT010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.334"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..337
                     /locus_tag="RD743_002975"
     CDS             <1..337
                     /locus_tag="RD743_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replisome organizer"
                     /protein_id="HDU0564717.1"
                     /translation="AKPVRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCE
                     SSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAIDQEAYLKKKALER
                     ANRQSKAPF"
     gene            349..618
                     /locus_tag="RD743_002976"
     CDS             349..618
                     /locus_tag="RD743_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564718.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
BASE COUNT          280 a          119 c          155 g          166 t
ORIGIN      
        1 ggctaagccc gtccgtcata aatacggaga gtataaaaat gttcttttgt cagatgagca
       61 gatggagaaa ctcaaaacag aattccctaa tgattaccaa gagcgaatcg aacgactgtc
      121 agagtattgt gaatcatctg gtaagactta taaaaactat ttggcaacta ttcgaagttg
      181 ggcaaggaaa gaaaaaagtg aacctaagaa cgcaagcagt ggatacaagc gcacaggaag
      241 acgagagaag cttcctgaat gggcaatcga ccaagaagcc tatctcaaga aaaaagcgct
      301 agaacgagct aatagacaat caaaagcacc attctaagag gtggaaaaat gaagatcgat
      361 tatctagaac taattaatga aatagcgaat tataaaaagg gcgaggaatt agacgtcctg
      421 agagacgtat atgatcaact cgaagaagct ggaattgaag gaattaagaa tgatcgttcg
      481 agttggagta agctcagata ctatttcgca ctttatatcg atacaacaca attaagaaat
      541 ttagcatata ccaaattact atttgttgat tgtgttaaag gcttgcaaaa acatcttaat
      601 gaacttgagc aggtgtaatc agatggacct aaagacattt acagcacaga ttgaactaat
      661 gcatcaagaa gctttaagac aaagtgtatc gtacgaagac aagtggctca acacgttcca
//
LOCUS       DAPCMT010000323          713 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPCMT010000323 DAPCMT010000000
VERSION     DAPCMT010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.335"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            54..410
                     /locus_tag="RD743_002977"
     CDS             54..410
                     /locus_tag="RD743_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDU0564719.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            400..666
                     /locus_tag="RD743_002978"
     CDS             400..666
                     /locus_tag="RD743_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0564720.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
BASE COUNT          287 a           97 c          146 g          183 t
ORIGIN      
        1 gaatttttat gttatatact ataatataaa atcaatatat gatataattg ggtatggaaa
       61 aaatatcttt tgaaacattt aaaagaccta atggtcatga tgaattttta gaatggttgg
      121 aaacattacc aaaaaaagat agtgcgaagc ttttacgtac aatagaagaa acagaaaaga
      181 atgggatgct agtagctcaa cggttgaaat gggtgaaaaa attagacact aatttgtacg
      241 aattaagaag taaggtagga tcaaacatcc aaagagctat ctatttccat gtggtcaatg
      301 gaaggtatgt cattacccat ggatttacta aaaaaacgga gaaaacacca ataagtgaac
      361 ttaaacatgc aaaagaactt agagaggagt ggttcacaaa tgatgattaa aaaattgatt
      421 gaagaaaagc aaaaagacaa agattttgca gaagcatatg accgtgaagg aagaaaactc
      481 gaaactgcgg ttgctcttta tcatgcacgg gaggaagcag gcctaactca agcggagctt
      541 gcacaaaaag cacatacaac tcaatctact attgctcgaa ttgagagagg ggataacgta
      601 tctttcgata aacttgatga tatcgctagg gcattaggca aaaagctggt agtagaattt
      661 gcttaaaagc gagttgaaaa tatacctgta ataggctcct aactaatcaa tta
//
LOCUS       DAPCMT010000324          696 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPCMT010000324 DAPCMT010000000
VERSION     DAPCMT010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.336"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(54..629)
                     /locus_tag="RD743_002979"
     CDS             complement(54..629)
                     /locus_tag="RD743_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDU0564721.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAPCMT010000325          695 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPCMT010000325 DAPCMT010000000
VERSION     DAPCMT010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.337"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..429)
                     /locus_tag="RD743_002980"
     CDS             complement(<1..429)
                     /locus_tag="RD743_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0564722.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIF"
BASE COUNT          211 a          119 c           95 g          270 t
ORIGIN      
        1 aaaaatttga tattttttta gatttctttt tagtctttgt aatactttat aagccacaga
       61 ataactcacg tgatgctgtt tagcaaaatt ttctactgag atatattttt cctgaaagaa
      121 agccaatacc attttcatag ataaagaatc cgttatataa cgagataaca gagctttgct
      181 agaacatttt ccattaattt ccagacatat atccaattca ctgacggtta ctcgcaaatt
      241 tttctctaga tcgaactttt gtaaatcatg tttcagctta tcgatcgttt ttttcaagag
      301 aaatgtcgat agcgacaact cttcttgaac attcgatatt gactctgata aatttgagct
      361 tgcttctaaa tatttaagta atgaataact tctgtgatct tctttgctta aaaaaaactc
      421 ataattcatt agtcatcagc tctttctacg tatattactt tccatgcatt caccaactag
      481 ctggggataa aactgatttc ctggatccga gtcactgttg tcagcgattc ttgtttgtat
      541 aatatacatc gtcctgtctt ttttttaaca ttttggagag cgttttattt ggcctttctc
      601 gcgatcaaac ctgttattag aattatatca tttactagta tgcattttta ttaataaaat
      661 ggtatatttt atattgttat aaaaagagcc caacc
//
LOCUS       DAPCMT010000326          654 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPCMT010000326 DAPCMT010000000
VERSION     DAPCMT010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.338"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..626)
                     /locus_tag="RD743_002981"
     CDS             complement(<1..626)
                     /locus_tag="RD743_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564723.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          186 a          132 c          128 g          208 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgtt
//
LOCUS       DAPCMT010000327          608 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPCMT010000327 DAPCMT010000000
VERSION     DAPCMT010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.339"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            291..>608
                     /locus_tag="RD743_002982"
     CDS             291..>608
                     /locus_tag="RD743_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDU0564724.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEH"
BASE COUNT          215 a           78 c          123 g          192 t
ORIGIN      
        1 gctaaagtta gaaaaggcag aaattacaat tttgctgtcc agtttaatat ttctagtgag
       61 tttgatgaaa aaacttatag agaaaaaaaa ttaggttaat gcaaaatata tcttactcgt
      121 ttgaagatgc ttcagacgta tgctaagata tatagtgcct tgggagcaag gtattaatct
      181 cctttagagg ttaattagct tgtgatcgcc tatcaatttt tttggtaggc gattcttttt
      241 tttgaaacaa aaaagcaaaa attatttttt aattagagag gtgcaatgcg atgaaatact
      301 ttttttgtgc agtattcgaa tggaaaggaa atgaatatgc agttaaattc ccagattttc
      361 cagacttata tacacatggt gaaactttag aagatgcttt tgagatggcg caagaagtat
      421 tggaaggata cttggtgctt ttagagttgc aagaaaaaga aattccatcg cctacgcctg
      481 ttcaagagtt aaatgcaaga atcaagcgat ctgagaatgt taatttattt gtcgtaaaag
      541 ctaatacgaa agttgctaaa gctcgaaaca agcaaaatta tgatcgaaaa tcagtgactg
      601 tggaacac
//
LOCUS       DAPCMT010000328          594 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPCMT010000328 DAPCMT010000000
VERSION     DAPCMT010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.341"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(23..>594)
                     /locus_tag="RD743_002983"
     CDS             complement(23..>594)
                     /locus_tag="RD743_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0564725.1"
                     /translation="KISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPW
                     IIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTI
                     IGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFF
                     TLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          205 a          106 c           68 g          215 t
ORIGIN      
        1 ccaaaaaaaa ttactttcat cattaattta cctcaatgtc gttaattaag ttaataatat
       61 acttatcttt ttcaaaagta tattggggtt ccattttctt ttccaaaagc gtgaaaaatt
      121 ctttaatctc ctctaaatat gcatcttcaa taatagtatg tgaataatta ttatcatgca
      181 taacctcatc gtacaattga atatctgtca tactagaagt ttcttcattc catgaaaata
      241 gagaatccgg gttaccttcc cattctatta aaatcttttc accaattata gttaaatttc
      301 ttttagctac tcttgaaaca acatctacac ttaaagttcc aactatacca gtttcatgct
      361 caataataag agtgaaactg tccgaataat caatatcaag attggataat ttctgggtca
      421 ttacagtaaa cgatttaact ttcccaaatg tatctataat ccaaggtaat tcaatagcga
      481 atatttctct acacccatta gtttttttgt ttgaaacaaa aaaattgcga taatcttccc
      541 aaggatgcca gtcgggtaaa tactgtccta catggtattg ataagaaatt tttt
//
LOCUS       DAPCMT010000329          581 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPCMT010000329 DAPCMT010000000
VERSION     DAPCMT010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.342"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            67..>581
                     /locus_tag="RD743_002984"
     CDS             67..>581
                     /locus_tag="RD743_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564726.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVS"
BASE COUNT          201 a           91 c          121 g          168 t
ORIGIN      
        1 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
       61 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
      121 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
      181 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
      241 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
      301 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
      361 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      421 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      481 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      541 cttccgttgg gaagtggaat gcagtatgag agctcggttt c
//
LOCUS       DAPCMT010000330          580 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.343, whole genome shotgun sequence.
ACCESSION   DAPCMT010000330 DAPCMT010000000
VERSION     DAPCMT010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.343"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            37..>580
                     /locus_tag="RD743_002985"
     CDS             37..>580
                     /locus_tag="RD743_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDU0564727.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLD"
BASE COUNT          227 a           58 c          129 g          166 t
ORIGIN      
        1 aacaaattat tataaatatt agaggtataa aaagttatga aacacaaggt aagtgtgata
       61 gtaccaattt acaatgttga aaaatattta agaaaatgtg tggatagttt attgagtcag
      121 acattaaaag agatagaaat cgtattagtt gatgatggtt cgccagatgc ttcgggggaa
      181 atagcagatg agtatcaaaa aaaatattct aatgtaaaaa caatacatag agaaaatggg
      241 gggttaggac cggcaagaaa tacaggaatt gagaatgcga caggagaata tgtggctttt
      301 cttgattcag atgattgggt agaaagtgat atgtatgaaa aattatactt agttgcgtcc
      361 aaagaaaatg ctgatatagt agttagtgga cattgtgatt ttgcgaatga caaagctatg
      421 gtgaaaaagc aacatccttt gagtgggaat gtatatgata cgcctaatag tattaaaaat
      481 gtacgaaaaa atttatttgg acatagtcca aaagatcaag aagtagaggc cttcccgatg
      541 tcagtatgta tgtctttgta taataaaaat ctgctagata
//
LOCUS       DAPCMT010000331          576 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPCMT010000331 DAPCMT010000000
VERSION     DAPCMT010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.344"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            427..>576
                     /locus_tag="RD743_002986"
     CDS             427..>576
                     /locus_tag="RD743_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDU0564728.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTK"
BASE COUNT          211 a           90 c           84 g          191 t
ORIGIN      
        1 ttcgtttcta aatattccaa tttgggaatc caataaaaat tttcgttcaa atacgtgtct
       61 attcgaactc ttttctagtt gttaattatg aagggggaag aaataaaaaa tacaatctat
      121 acacgaataa gaatattatc caaaaggagg ttttagaaaa gatttattat acgtaaaaag
      181 ctcaagacat tttcttagcc ataaggacat gtttgatatt taagaagata tatgaatttt
      241 cttttctaag tataaagata actatccatt atcaactaaa aaaacatttt tgaaaataaa
      301 catcagcagt cctatcaatg catgtgagct tatagaagtt attcgcagcg cagcataatt
      361 gcattagctt cttttcgtgt tttttgcctc ttttcccttt aaatcttaat ggaggaggtg
      421 tgcaatatgt cagataacga cgaacgcgta aatgcccagt tgattcgatt ctttgaaaag
      481 gtcattttga acaccgctaa tacttattat aaaaaacaag aagaaattca aaaacatgaa
      541 caacttgatc aactcccacc atatttgttt actaaa
//
LOCUS       DAPCMT010000332          563 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPCMT010000332 DAPCMT010000000
VERSION     DAPCMT010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.345"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..504
                     /locus_tag="RD743_002987"
     CDS             <1..504
                     /locus_tag="RD743_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564729.1"
                     /translation="FQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADF
                     RMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNE
                     VILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVR
                     YMFNKIT"
BASE COUNT          176 a           85 c          115 g          187 t
ORIGIN      
        1 tttcaaaatg cagttatgga ggggatacgc tatggctgtg aacaaggatt gtatggttgg
       61 aatgtgacgg actgtaaaat ctgttttaag tatggcttat actatagccc tgttagtacc
      121 ccagcagatt ttcggatgct tgctcctatt gtattggaac aagtcttaaa aaaagctgga
      181 acagaattgt tagagccata tcttagtttt aaaatttatg cgccacagga atatctttca
      241 cgagcataca acgatgctcc taaatattgt gcgaacatcg tagacactca attgaaaaat
      301 aatgaggtca ttcttagtgg agaaatccct gctcggtgta ttcaagaata tcgtagtgat
      361 ttaactttct ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt
      421 actaccggtg aacctgtttg ccagccccgt cgtccaaata gtcggataga taaagtacga
      481 tatatgttca ataaaataac ttagtgtatt ttatgttgtt atataaatat ggtttcttgt
      541 taaataagat gaaatatttt tta
//
LOCUS       DAPCMT010000333          555 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPCMT010000333 DAPCMT010000000
VERSION     DAPCMT010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.346"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..119
                     /locus_tag="RD743_002988"
     CDS             <1..119
                     /locus_tag="RD743_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDU0564730.1"
                     /translation="NDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            120..>555
                     /locus_tag="RD743_002989"
     CDS             120..>555
                     /locus_tag="RD743_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDU0564731.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSA"
BASE COUNT          210 a           66 c          105 g          174 t
ORIGIN      
        1 ctaatgatat gtatgagaag gagttgaaat atttctttga aaacgtagta acaggcaaag
       61 aaaactggaa tgatttatat catgcaaacg aagtattgaa aatagctgag gagaaataaa
      121 tggaaatttt atttactatt tgtggtagag ctgggtctaa ggggtttaaa aataaaaatt
      181 tgaaatattt ggcaggaaag cccctagtcc atttcgcttt agcaatgata gatatctatc
      241 aacaaaaaca tcctgaacat aattgcacga cttgtttgaa tacggacagt aatgagttag
      301 tagaattgac gactaagtta aatccgtctg tgaaaattgt agaacgtaaa cctgaattag
      361 ctacagatac tgtagggaaa attgaagtga tacgtgattc attcttacaa ctagaaagag
      421 aagataatat atttgattta attatagatt tagatttgac ttcaccttta cgtgaagttt
      481 ctaatcttga agaattaata gatgaacatc taaaaaattt agataaagac gtctactttt
      541 cagttgtttc tgcaa
//
LOCUS       DAPCMT010000334          538 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPCMT010000334 DAPCMT010000000
VERSION     DAPCMT010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.347"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(18..371)
                     /gene="tnpB"
                     /locus_tag="RD743_002990"
     CDS             complement(18..371)
                     /gene="tnpB"
                     /locus_tag="RD743_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDU0564732.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(361..>538)
                     /locus_tag="RD743_002991"
     CDS             complement(361..>538)
                     /locus_tag="RD743_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564733.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          155 a          120 c           92 g          171 t
ORIGIN      
        1 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
       61 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      121 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      181 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      241 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      301 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      361 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      421 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      481 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatc
//
LOCUS       DAPCMT010000335          524 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPCMT010000335 DAPCMT010000000
VERSION     DAPCMT010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.348"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(126..425)
                     /locus_tag="RD743_002992"
     CDS             complement(126..425)
                     /locus_tag="RD743_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDU0564734.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          166 a           99 c           62 g          197 t
ORIGIN      
        1 atataaagaa acatctgttt ctttatttag aataaaaaaa tacagactct gttcaaataa
       61 atgaacctag cctgcattat gagattgact atacttttta attcattgag atgaataact
      121 cgtttttata aatgcccaaa agtaatcggt gccgaagcca ttcctcgacc taattctttt
      181 tcaaattttc ctattgttga tatctccata taaaacggtg atactggttt agacaattct
      241 cccctgattg ctaacggacg taaactacgt tccaaattat ttatgacttt aggataatac
      301 tgaccttttt ctaagcctgt ctttgcgtct atcaaaagta tacgttcctt ttctttgatc
      361 tctggattta aaacgagatc atataatttt agtaccatgt tttctatttc ttcactttta
      421 gacatcacga tttcctccca aaaatatatt tcttcccact acattctata aacaaagtat
      481 acgctacatc tagatttttt taaatcatta ctggattgtt aaaa
//
LOCUS       DAPCMT010000336          522 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPCMT010000336 DAPCMT010000000
VERSION     DAPCMT010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.349"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
BASE COUNT          152 a           80 c           76 g          214 t
ORIGIN      
        1 catggaaata tttggctttt atgatacggc atttcacatt aatcgtgcgt taagtttaga
       61 atctattttt agctccccta ttaattttga aacgtttaga tcatatggca tgcaagtaaa
      121 taatttttac ccatggttaa ctctatatcc tttattttta ttgatcaagt ttactaattt
      181 agcaataggc tataaccttt ttctttatat tgttacgctc attactctat ttatatgtca
      241 ttatgtaatg tatgaaatta ccaaaaaaca tgttactagc tcctttttcg ctattatata
      301 tacaacatct tcttttagga gtgtagaaat atttcttcga ggggcaatgg gggaattact
      361 cgcaatgtca atacttcctc ttattttact cgggtttata aagctttacg actcaaaaaa
      421 agaatcttgg gttatgttgg cgattagcat gactttatta atttacactc atgttctttc
      481 tgtagctatg actatggtaa tgattataat tgctttaatt at
//
LOCUS       DAPCMT010000337          510 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPCMT010000337 DAPCMT010000000
VERSION     DAPCMT010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.350"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            1..510
                     /locus_tag="RD743_002993"
                     /pseudo
     CDS             1..510
                     /locus_tag="RD743_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           88 c           92 g          118 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag
//
LOCUS       DAPCMT010000338          509 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPCMT010000338 DAPCMT010000000
VERSION     DAPCMT010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.351"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
BASE COUNT          228 a           70 c           82 g          129 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tatgatataa aagttgctac
      481 ggcagccaaa aatgaatcgg gaaacactc
//
LOCUS       DAPCMT010000339          502 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPCMT010000339 DAPCMT010000000
VERSION     DAPCMT010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.352"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            64..>502
                     /locus_tag="RD743_002994"
     CDS             64..>502
                     /locus_tag="RD743_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HDU0564735.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYL"
BASE COUNT          200 a           69 c           83 g          150 t
ORIGIN      
        1 cttttagaaa aagcaaaaaa aaaagaaaaa atcttattct tgataacaag ctacgtgacg
       61 tatatgccta acaaaaatag aacttttgaa tcaatcttaa atgaaggttt aaccacagaa
      121 ccggcatcca ttatttttga tgaagttatc gatgacgttg aagatagtaa gtatcataaa
      181 aatttaaaag aactttttta ttttgcactc aacaatggac caagtttgtt cactggtata
      241 tcagtacctg atagtgataa tccattaatt atggcaaaaa tttacttaac aaaatggtgt
      301 aataaataca tcagggatag gaataaccca gcattaaaaa aacctcttaa aacatttggc
      361 gaaaaagatg ctgctttaac tactagagta gcagcaaatg ctaatattga tgatcaaaaa
      421 gtattgaatg actacttgcg tggacatttt ttatatatga gcgctgagaa catgaacgga
      481 agtattttag aggaatattt ag
//
LOCUS       DAPCMT010000340          490 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPCMT010000340 DAPCMT010000000
VERSION     DAPCMT010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.353"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            45..>490
                     /locus_tag="RD743_002995"
     CDS             45..>490
                     /locus_tag="RD743_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0564736.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQ"
BASE COUNT          177 a           61 c           87 g          165 t
ORIGIN      
        1 cgttactatt tttttcttaa gtattttgat ttgaggtgaa agaattgttt gaatttaaca
       61 atgtttcttt ttcatataag gatgttaagg aacaagcgct tgttgacctt aatttttcaa
      121 taaaaaaagg cagtcttaca ttgattactg gttcttctgg gagtgggaaa tccacactat
      181 taaagttaat gaatggactt attccaagac tttatgacgg aaaattggtt ggggaaattt
      241 tatttgaaaa taactcgcta caatccttaa cacaagaaag tatatcaaaa aatattggct
      301 acgtaagtca agatcctaga ggacaattct ttacaacaaa tacaacgagt gagttagtat
      361 tttcgatgga aaactttgga tttacaaaag agcatatgga taaaagaatc aatgaattag
      421 ctgaattatt agatctttca aatatattag ataaaaacat tttttcgcta tcaagtggtg
      481 aacggcagaa
//
LOCUS       DAPCMT010000341          490 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPCMT010000341 DAPCMT010000000
VERSION     DAPCMT010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.354"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(27..425)
                     /gene="spxA"
                     /locus_tag="RD743_002996"
     CDS             complement(27..425)
                     /gene="spxA"
                     /locus_tag="RD743_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDU0564737.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          141 a          101 c           84 g          164 t
ORIGIN      
        1 tcttttccaa gtttcgtatt atccttctat aatccagcca tcaattgtgc ttgacgtaat
       61 tctaattgtc gtacatcgcg cggtaggaac cgacgaattt catcttcatt aaaaccaact
      121 tgcagacgtt tatcatcaat catgattgga cggcgaagca atccaggatt gttttgcaca
      181 agttccaata attcttgtaa cggcagatca tctaaatcca tgttcagttt ttggaatact
      241 ttggaacgtg tcgagatgat ttcttctgtt ccgtcttctg tcatttgtaa aatcgctttt
      301 aattcagaac tgttcaatgg ttctgaaaaa atattacgtt caacaaatgg aatctgatgt
      361 tcttgcaacc acgcacgagc ctttctgcag gaagtacaac ttggggaagt atataatgtt
      421 aacaacgtgc accactcctt ttcgtgataa gaatcacttt tttgtaacag aagatcataa
      481 taatctccca
//
LOCUS       DAPCMT010000342          489 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPCMT010000342 DAPCMT010000000
VERSION     DAPCMT010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.355"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
BASE COUNT          186 a           79 c           80 g          144 t
ORIGIN      
        1 ttatggttac actcctttgt tttctttcgt cggaaataca atttgagtgt accataaatg
       61 cctttttatt tttctaactt aattttacaa ttcgcgatat attaaacaac gttgtcgcgc
      121 ttcattgaaa cattttatga aaattccaaa aaagagttga cacatgtttt tagacgtgat
      181 aagataacta agtcgttaaa aagaaccact tgcgaagctt gattctatcg aaaaaaagct
      241 tcaaaaaagt tcttgacaaa taacaaacga taagttatga taaatgagtt gctgaaagat
      301 ttcgaaaaac gaaatcgaaa aaaactttta aaaaagagtt gacaatcgct tgtctctctg
      361 atatgatata aaagttgcta cggcagccaa aaacgaatcg ggaaacactc ccgatgaagt
      421 agacctttga aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc ttgattcaat
      481 tcaagacaa
//
LOCUS       DAPCMT010000343          485 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPCMT010000343 DAPCMT010000000
VERSION     DAPCMT010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.356"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(62..259)
                     /locus_tag="RD743_002997"
     CDS             complement(62..259)
                     /locus_tag="RD743_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0564738.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DAPCMT010000344          462 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPCMT010000344 DAPCMT010000000
VERSION     DAPCMT010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.357"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..430)
                     /locus_tag="RD743_002998"
     CDS             complement(<1..430)
                     /locus_tag="RD743_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDU0564739.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAPCMT010000345          456 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPCMT010000345 DAPCMT010000000
VERSION     DAPCMT010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.358"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..>456)
                     /locus_tag="RD743_002999"
     CDS             complement(<1..>456)
                     /locus_tag="RD743_002999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564740.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQY"
BASE COUNT          152 a           77 c           58 g          169 t
ORIGIN      
        1 catactgaat ttctgaaaat cgaatttttc tcccagttga tagatcaagg ggcagaaaac
       61 cttttattac attgtgagtg tcaccatcat ttacaatgtc attaggaatt ttgattttag
      121 aattctgtgg agcacctatt ggaagataat aattcattat aattttgtag tcttcatgag
      181 gaaataaagt acttttagga ttgctaagat attctcgtac cgaatctaag aatggcttat
      241 ccattgaatc ttcatcccca tttaaactaa aaaacattcc tacaattgct tttgcaactc
      301 taagtgaatt cattttcttt attgaatgtg ttaaatcaga cttaccattc tttaaacttg
      361 tattgttata aaggaaattt ttattgtctt ctgcaaatct aatatattcc tcaacatact
      421 cactaccaaa aaaactatta catttactac ataacg
//
LOCUS       DAPCMT010000346          452 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPCMT010000346 DAPCMT010000000
VERSION     DAPCMT010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.359"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>452
                     /locus_tag="RD743_003000"
     CDS             <1..>452
                     /locus_tag="RD743_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564741.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAPCMT010000347          451 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.360, whole genome shotgun sequence.
ACCESSION   DAPCMT010000347 DAPCMT010000000
VERSION     DAPCMT010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.360"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
BASE COUNT          152 a           73 c           95 g          131 t
ORIGIN      
        1 tttcattgca ataattgcaa caaagaaatt tcatcggggg aagaggtttg gacaaaaatg
       61 gaaatttccg ccaaaagtaa ctgctcctca actgaaatca attaaagaac tagaatttga
      121 gaatgcactg gtaatttgta tgacatgtac tgatgaagta tttcgaaaac atttttaaag
      181 gttgagatgt atatagattt aaccaatcat ttatctctct cggaaaagga atattaacta
      241 accagcgggt tatcaaaaat aggtaggaaa actgtactta atggcttatt tcgcgctgat
      301 tatgagaata tcagtctgtt tgattaattt ttagggttca aaatacagag acaaactcca
      361 ggtcgggttt cgccacaagc gaattacccg atctggagtt ttgtctattg caggaaggcg
      421 tagccggact gcaggaataa agcccccggt t
//
LOCUS       DAPCMT010000348          437 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPCMT010000348 DAPCMT010000000
VERSION     DAPCMT010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.361"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..308
                     /locus_tag="RD743_003001"
     CDS             <1..308
                     /locus_tag="RD743_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564742.1"
                     /translation="IRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLI
                     EGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          172 a           63 c           62 g          140 t
ORIGIN      
        1 aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt aaattgctca
       61 tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga actatttatt
      121 caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa agaaaagagc
      181 aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac aactataatc
      241 aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca ttagaatcaa
      301 tgttttaact tgatcaacag aatgggcttt tccatttaca caaaattatt gacgctcccg
      361 acaaaattat gatcttgcgt aaagttataa aatattaata aaaatatttt attaatgaaa
      421 agcttggtag cacggtt
//
LOCUS       DAPCMT010000349          435 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPCMT010000349 DAPCMT010000000
VERSION     DAPCMT010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.362"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>435
                     /locus_tag="RD743_003002"
     CDS             <1..>435
                     /locus_tag="RD743_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564743.1"
                     /translation="VFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVS
                     AAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          175 a           65 c          101 g           94 t
ORIGIN      
        1 agtctttgca gtgaaagacg gcgtaagctt accagaagat aaacttccag tgttgaaagc
       61 aaaagatggt tatacagatg cgaaatggcc agaggaagca actcaaccaa ttacagcaga
      121 tgatacagaa tttgtatcaa gtgcaacaaa actggatgat aaatctgacg ctgacaaata
      181 taatcctgaa ggtcaaaaag tgactacaga attgaataag gaaccagacg catttgaggg
      241 aattaagaac aaagaagatt taccaaaaga tactaagtat acttggaaag aaaaagtaga
      301 tgttagcgca gctggaaata aaaaagggac tgttgtagtg acatatccag atggatcatc
      361 tgatgaagtt gaagtagatg ttacagtgac agacaatcgt tctgacgccg ataaatatga
      421 gccaacagta gaagg
//
LOCUS       DAPCMT010000350          429 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.363, whole genome shotgun sequence.
ACCESSION   DAPCMT010000350 DAPCMT010000000
VERSION     DAPCMT010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.363"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>429
                     /locus_tag="RD743_003003"
     CDS             <1..>429
                     /locus_tag="RD743_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564744.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFILST"
BASE COUNT          157 a           75 c           88 g          109 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgagaaa aacaatttgc ttattctgcc catacttcat gcgacgacaa tggatttatt
      421 cttagcaca
//
LOCUS       DAPCMT010000351          427 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPCMT010000351 DAPCMT010000000
VERSION     DAPCMT010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.364"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..>427)
                     /locus_tag="RD743_003004"
     CDS             complement(<1..>427)
                     /locus_tag="RD743_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564745.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT          125 a           76 c           87 g          139 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaa
//
LOCUS       DAPCMT010000352          424 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPCMT010000352 DAPCMT010000000
VERSION     DAPCMT010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.365"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..172)
                     /locus_tag="RD743_003005"
     CDS             complement(<1..172)
                     /locus_tag="RD743_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0564746.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISI"
     gene            complement(191..352)
                     /locus_tag="RD743_003006"
     CDS             complement(191..352)
                     /locus_tag="RD743_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDU0564747.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          143 a           76 c           46 g          159 t
ORIGIN      
        1 ttattgatat tgaagaatta gaactttcat ataaagcctt ttctatttca ttgcttgatt
       61 tcattattgt taaacaagaa tacaagcaag aaagaactat agttaatatg ataaaaatta
      121 ttaaagttct atttttcttt cttgtaatat atgctatagc attttttatc actttcctaa
      181 ctactcctct ctattttctt actccataac taattgcatc cactggacaa gcatttttac
      241 atcgtccaca tctgatacac tccagatgat tgcaattctc aactgaatct ttattcattt
      301 ggcaaacttt tttacatttt ccacaattaa tacacttatc cttatcctga atcggctaca
      361 ctagactaga cagaaaaaat aaggtgtgta gaataataaa aaacacactg gaggattttt
      421 catg
//
LOCUS       DAPCMT010000353          422 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPCMT010000353 DAPCMT010000000
VERSION     DAPCMT010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.366"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            105..>422
                     /locus_tag="RD743_003007"
     CDS             105..>422
                     /locus_tag="RD743_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDU0564748.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNL"
BASE COUNT          162 a           50 c           75 g          135 t
ORIGIN      
        1 aaaatgatgt ttgtacttac ttaaattagg tataattagt atatgttttg tgagaaattt
       61 agacttacaa taagcagtga atttataatc aacgtatagg agacatggat gaaatgaaac
      121 taattggtat agatttagac ggtactcttt tgaattctga ccagagcatt agtcaaaaaa
      181 atgcaagaac aatgaaacat ttaccggaag actgttttcc attcatttgt tctggaagag
      241 aagtagaaga cattaataat attctcaaaa aatctggttt aaggatacca gcagtagggt
      301 taaatggttc tgttggattt gatggagaaa aaaagatatt tgagtcttcc ttcgataaaa
      361 atgatgtaaa aaaaattgat tctgtcctat cgaaatttcc aaccaaaata tatacaaatc
      421 tc
//
LOCUS       DAPCMT010000354          419 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPCMT010000354 DAPCMT010000000
VERSION     DAPCMT010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.367"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..193)
                     /locus_tag="RD743_003008"
     CDS             complement(<1..193)
                     /locus_tag="RD743_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0564749.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREE"
BASE COUNT          137 a           71 c           52 g          159 t
ORIGIN      
        1 tttcttcgcg ccccagaaga tagtctacag agacatcgaa atagtcagca atttcttgca
       61 aacgttctgt agaggggttt gagttcttaa gtctatataa gacgttttta ccatagccta
      121 ggtcttcttc aactttattt aatgattttc cctgtttttt cgctaactct tttattctct
      181 caaacgttat catatcaata ttcctttcgt tcaagaaaaa atatttaact ttatttgtta
      241 aaattcgttg actattttaa caaaaggtgt taatatcatt cttgtaaaca agtttaacaa
      301 ctaaaaagac aacaaaaaac actattgatt aataaatgcc aaccgccaag aaagctttta
      361 aaatcaatgt ttatatgtct tatttaacta tgttctgatt ttaacactgt atgttaatt
//
LOCUS       DAPCMT010000355          412 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPCMT010000355 DAPCMT010000000
VERSION     DAPCMT010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.368"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            186..>412
                     /locus_tag="RD743_003009"
     CDS             186..>412
                     /locus_tag="RD743_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564750.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNR"
BASE COUNT          158 a           49 c           82 g          123 t
ORIGIN      
        1 ttggtgtaat taattgtaga gaatagcctt tgagcaattg aagcgataca attatttaga
       61 aaatgaactt cctccagcat actacatgat tccttacggg acgatgttaa agaagaaagc
      121 aagaaatttt agaagtatta gtagataaac taatgggctt ttctcaattt aagggagtag
      181 cccaaatgtt tatcaaatat ggtgcagata cagtagcaac catgtttaca gaagaatatc
      241 aagtagacta ttatgaagta atagataaat gtgtacattt tgtttatgaa aaagtgtgga
      301 attttatgaa ttttgcgata agtgaagaaa tcttacgagt acaagaaaac agaacaggaa
      361 tatttaccag agacagaaag cttaatttga ttgcgcatta tatacggaat cg
//
LOCUS       DAPCMT010000356          384 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPCMT010000356 DAPCMT010000000
VERSION     DAPCMT010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.369"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            33..236
                     /locus_tag="RD743_003010"
     CDS             33..236
                     /locus_tag="RD743_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HDU0564751.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          150 a           43 c           78 g          113 t
ORIGIN      
        1 aatgattatg aaaaagaaag aaaaagaaag tattgaaaca ttgtgttatt ctaggaatat
       61 taggaacttg tctagctggc attggtacag gaatagacgt tgacgcagct acttactatg
      121 gaaatggtct ttattgtaac aaagaaaaat gttgggtaaa ttggggacaa tcatggtcag
      181 aagggttaaa acgttgggga gataatcttt ttggtagttt tagtggagga cgataatgac
      241 ttcaataaac attgatcaca caaaaaaagt catacatgaa ttatacaata gtttaatgac
      301 acgacctaat cagacgaaag cattattaga tattactgat gttttatttc aggtatataa
      361 aaaaattgat acagcaaaat atcc
//
LOCUS       DAPCMT010000357          331 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPCMT010000357 DAPCMT010000000
VERSION     DAPCMT010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.370"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>331
                     /locus_tag="RD743_003011"
     CDS             <1..>331
                     /locus_tag="RD743_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HDU0564752.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DAPCMT010000358          321 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPCMT010000358 DAPCMT010000000
VERSION     DAPCMT010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.371"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(69..321)
                     /locus_tag="RD743_003012"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="RD743_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAPCMT010000359          307 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.372, whole genome shotgun sequence.
ACCESSION   DAPCMT010000359 DAPCMT010000000
VERSION     DAPCMT010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.372"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(77..>307)
                     /locus_tag="RD743_003013"
     CDS             complement(77..>307)
                     /locus_tag="RD743_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564753.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           93 a           46 c           57 g          111 t
ORIGIN      
        1 gatgttggtg aagttcacca acatcaaaaa gtatagagcc gatagaagga ctgaccttct
       61 gtctcagatt tttttgttat ttcacttgaa tcaattgatt gatcaaaaaa atcctgattt
      121 tcatgttctc aaatgattta aatccataag ataatcgttt cagtgtcttg atatggggaa
      181 tcttggcctc tattttgcca ttagaatacg ggtaaatcat agcattgtaa ataccttctt
      241 catacgttaa aaggttttga agtttcgtgc gaagttgtgc atcaagtgtt tcaggaagat
      301 tttttaa
//
LOCUS       DAPCMT010000360          302 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPCMT010000360 DAPCMT010000000
VERSION     DAPCMT010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.denovo.373"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..179)
                     /locus_tag="RD743_003014"
     CDS             complement(<1..179)
                     /locus_tag="RD743_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDU0564754.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSAS"
     gene            complement(197..>302)
                     /locus_tag="RD743_003015"
     CDS             complement(197..>302)
                     /locus_tag="RD743_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDU0564755.1"
                     /translation="LSENDTVFSFDGKEISKETMRKAIAIAKALEENE"
BASE COUNT           96 a           63 c           40 g          103 t
ORIGIN      
        1 gaggcgctga aatgtgcaag ttcatgagat aaatgaaagg cttctgcagt ttcgccatat
       61 agattttcat tcaaaaaaat gattctaggc ttcggataat agaagccagg ctctttcatt
      121 tccatgtaaa ctactttcaa attgtattcg ctcaacattt ctttcaattt caaatacata
      181 caacccatca ctccaactat tcattttcct ctaaagcttt agcaattgca atcgctttac
      241 gcattgtctc cttagatatt tcttttccat caaaagaaaa aacagtatcg ttttctgata
      301 aa
//
LOCUS       DAPCMT010000361        13355 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.guided.077, whole genome shotgun sequence.
ACCESSION   DAPCMT010000361 DAPCMT010000000
VERSION     DAPCMT010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.guided.077"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            125..1210
                     /locus_tag="RD743_003016"
     CDS             125..1210
                     /locus_tag="RD743_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0564756.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            1207..3099
                     /locus_tag="RD743_003017"
     CDS             1207..3099
                     /locus_tag="RD743_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0564757.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            3106..3483
                     /locus_tag="RD743_003018"
     CDS             3106..3483
                     /locus_tag="RD743_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0564758.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            3637..4419
                     /locus_tag="RD743_003019"
     CDS             3637..4419
                     /locus_tag="RD743_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDU0564759.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(4545..5276)
                     /gene="erm(A)"
                     /locus_tag="RD743_003020"
     CDS             complement(4545..5276)
                     /gene="erm(A)"
                     /locus_tag="RD743_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDU0564760.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(5334..5393)
                     /locus_tag="RD743_003021"
     CDS             complement(5334..5393)
                     /locus_tag="RD743_003021"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDU0564761.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            5785..6447
                     /locus_tag="RD743_003022"
     CDS             5785..6447
                     /locus_tag="RD743_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDU0564762.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            6682..6825
                     /locus_tag="RD743_003023"
                     /pseudo
     CDS             6682..6825
                     /locus_tag="RD743_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            6975..7175
                     /locus_tag="RD743_003024"
     CDS             6975..7175
                     /locus_tag="RD743_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0564763.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7429..9108)
                     /locus_tag="RD743_003025"
     CDS             complement(7429..9108)
                     /locus_tag="RD743_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDU0564764.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9110..9322)
                     /locus_tag="RD743_003026"
     CDS             complement(9110..9322)
                     /locus_tag="RD743_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDU0564765.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9715..11445
                     /gene="efrA"
                     /locus_tag="RD743_003027"
     CDS             9715..11445
                     /gene="efrA"
                     /locus_tag="RD743_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDU0564766.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11442..13202
                     /gene="efrB"
                     /locus_tag="RD743_003028"
     CDS             11442..13202
                     /gene="efrB"
                     /locus_tag="RD743_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDU0564767.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         4433 a         2084 c         2688 g         4150 t
ORIGIN      
        1 gtataataaa taatagtagt taaagtagtt taagaggaga tattaaaggg atattttact
       61 ttaaataaaa attaacaagg tttataattt gtagtagtta aagtatttaa agaggtggga
      121 acatgtgaag gttcaaagga tagaagtgga gaataagccg tatccattgt atttattact
      181 agataaagaa taccagctaa tagaaccagt aatgaaattt attaaatact tagataatac
      241 tggtaagtct cctaatacca ttaaagcata ctgctatcat ttaaagttgc tgtacgagtt
      301 catggaacag agaggtgtta ttcttaatga tattaacttt gagttgttag cagacttcgt
      361 aggttggttg agatatcctt cagcatcaaa tgtaattgat cttcagtcaa aaaaagccat
      421 aagagaagaa acgacagtga atacaatttt aaatgtagtt atgagttttc ttgattattt
      481 aagtagatta ggagaattta aatcaattga tgtatttaaa caagcaaagg gaagaaattt
      541 caaaggattt ttacatcatg ttaataaggg tagataccaa aagaatgtct taaagttaag
      601 ggttaaaaag aaacagataa gaacattgag atcagaggaa gttaagcaaa ttattgatgc
      661 ttgtcatacg aaaagagata aattaatttt aatgcttatg tatgagggtg gtttaagaat
      721 cggtgaagtg ttatcgctta ggcttgaaga tattgtcact tgggacaatc aaatccattt
      781 aacacctaga gatgttaatg ttaatgaagc ttatattaaa ttaaggaagg aaagaacaat
      841 acatgtgagt aaagaactta tgtcacttta tacagattac ttgatatatg agtatagtga
      901 ggaattggag catgattatg tttttatttc cttaaaagaa ggctattttg ggaaaccact
      961 aaagtaccaa agtgttcttg atctagttag aagaatagtt aaaaggactg gaatagaatt
     1021 tacatcacat atgcttcgcc acactcacgc aacgcagcta attagggaag gatgggatgt
     1081 tgcgttcgtt caaaagagat taggtcacgc acatgttcag acaacgttaa atacctatgt
     1141 tcatctttca gatcaggata tgaaaaatga gtttaataaa tacctcgaga gaaaggagca
     1201 taagaaatga atgcttctag taaaaggaaa attattagtc agagtgagat tagcaaaaaa
     1261 atagctgtaa tgaatgaaga aatgcagggg ttttgggcta ataatagttg ggatataaga
     1321 aaatgtccac atccttctgc catagaatta agtaagaatc ctgctttaag gaatcgttgg
     1381 gttcgttttg aacgtgttaa aaatctgtgg ttaagaacag aattgaaata tttttatttt
     1441 taccatttaa acaatggaat atggaatgca aaaactgtct ggattagaaa aggaacagta
     1501 attaataaaa tgttagattt cttagattta aagtatccta gcattacttc aattactgaa
     1561 gttcctattg aaaaagcaat gacggagtat agaacttatt tgacaaaacg gggtgttaga
     1621 attaccacta ctaattataa gattactgct aatcaagaaa aaacacctgt aaaagctaat
     1681 tcctactatg ttactaatct aaaacaattt atggagtttt atgagaactt ttattttgat
     1741 ggagaggagt gggataaaga cgtttgggat agacgtaact tacctttgcc agatgataag
     1801 gttaacccaa cacaatatga atatacaatt aactttaaag ggtttcggaa tacatatttt
     1861 aaacaacttg taaaaagata ttgtaagttg agattgaaca tggatagctt ttcctatgta
     1921 agtgatattg cccaaagact taaagagttc tttaattttc tggacatgaa atttaaacaa
     1981 gttcagagag tacaccaatt aacgagagtg gaaattgaag catatttaag tgaactaaac
     2041 atgatgggaa taaaacctag tacaataact gggaggatct ctatattgga aggactattt
     2101 agtacccttc ttaggctaga atgggatgat gttccttcca aaatattaat ttattctgag
     2161 gactatccga aaataccaag agcaaaacca cgctttatag atgaattcgt cctagagcaa
     2221 ttgaacagtc atcttgataa attacccgaa tatatagcta cgatgactat gattgttcaa
     2281 gaatgtggaa tgaggataag tgaattgtgc accttgaaaa aaggctgtct attagaggac
     2341 aaagatggag atttcttttt aaagtattat caatggaaaa tgaaaaagga gcatatagtt
     2401 ccaatatcta aagaggtagc tttacttatt aaagttcggg aagataaagt ttcagaggaa
     2461 tttccagata gtgaatacct ctttccaaga aaagatggat cgccattaaa acaagaaaca
     2521 tttagaggtg agttaaataa attagcttat gagcaaaata tagtggataa atcaggtgag
     2581 atttatagat tccatgccca tgcctttcgc catacagtag gaacaagaat gattaacaac
     2641 gggatgcccc agcatattgt gcagaaattt ttggggcatg aaagcccaga aatgacaagc
     2701 agatacgctc atatctttga tgaaactcta aaaaatgaat ttactaaatt tcaggaaaaa
     2761 ctggttacca ataatggaga tgtgcttgat ctagatgaag ataatgaagt cgatgatgta
     2821 gagcttcaat ggttcaagaa aaatataaat gcacaagtgc ttccaaatgg ttattgtaga
     2881 ttgccagtag tagcaggtgg ttgtccacat gcgaatgcat gcttagattg cactcacttc
     2941 tgtaccagta agcaattctt accacagcac gaagaacagt tagagcgtac agaagagtta
     3001 ttagccatag ctaaggataa acaatggcaa agacaagtag agactaatag ccgtgttaaa
     3061 gagcgtttag aacaaatcat tggaagtttg acggggtaat tatcaatgga taaacaagtt
     3121 agaaatacaa cagaaattgt acgtttggcg aagcagaaat caaaaaagac aagggaaaaa
     3181 gtagacaaag cgatttctaa attttcgatt gaaggtaaag ttattaattt taattcaata
     3241 gcaaaggaag ctaatgtttc taaatcatgg ctttataagg aacacgatat taggcaaaga
     3301 atcgaatccc ttcgtgagcg tcaaataaca gcaaatgtag tctcaaaacc caagaaaagt
     3361 tctcgttcgg aggaaatcct tattaaaacc ttaaaaagaa gagtaatgga attagaaaaa
     3421 gaaaataaaa aattacagaa ccaaattcaa aaattatatg gagatctgta taataaagaa
     3481 taattattaa tctgtagaca aattgtgaaa ggatgtactt aaacgctaac ggtcagcttt
     3541 attgaacagt catttaggta tatgtccaat ctagggtaag taaattgagt atcaatataa
     3601 actttatatg aacataatca gttacgaggt gaaatcatga gcaatttgat taacggaaaa
     3661 ataccaaatc aagcgattca aacattaaaa atcgtaaaag atttatttgg aagttcaata
     3721 gttggagtat atctatttgg ttcagcagta aatggtggtt tacgcattaa cagcgatgta
     3781 gatgttctag tcgtcgtgaa tcatagttta cctcaattaa ctcgaaaaaa actaacagaa
     3841 agactaatga ctatatcagg aaagattgga aatacggatt ctgttagacc acttgaagtt
     3901 acggttataa ataggagtga agttgtccct tggcaatatc ctccaaaaag agaatttata
     3961 tacggtgagt ggctcagggg tgaatttgag aatggacaaa ttcaggaacc aagctatgat
     4021 cctgatttgg ctattgtttt agcacaagca agaaagaata gtatttctct atttggtcct
     4081 gattcttcaa gtatacttgt ctccgtacct ttgacagata ttcgaagagc aattaaggat
     4141 tctttgccag aactaattga ggggataaaa ggtgatgagc gtaatgtaat tttaacccta
     4201 gctcgaatgt ggcaaacagt gactactggt gaaattacct cgaaagatgt cgctgcagaa
     4261 tgggctatac ctcttttacc taaagagcat gtaactttac tggatatagc tagaaaaggc
     4321 tatcggggag agtgtgatga taagtgggaa ggactatatt caaaggtgaa agcactcgtt
     4381 aagtatatga aaaattctat agaaacttct ctcaattagg ctaattttat tgcaataaca
     4441 ggtgcttact tttaaaacta ctgatttatt gataaatatt gaacaatttt tgggaagaat
     4501 aaagcgtcct cttgtgaaat tagagaacgc tttattactt taatttagtg aaacaatttg
     4561 taactattga aaatagaaag aaattgttcc ttcgatagtt tattaatatt agtgacattt
     4621 gcatgcttca aagcctgtcg gaattggttt ttagtgaaaa gaacacgata ttcacggttt
     4681 acccacttat aaacaaaaga tcgatacttt ttgtagtcct tctttgaaat caatggttga
     4741 tgtcgttcaa gaacaatcaa tacagagtct acacttggct taggatgaaa atatagtggt
     4801 ggtacttttt tgagcatttt tatatccatc tccaccatta atagtaaacc caaagctcgt
     4861 tgcagatttt gcaatctttt cgcaaatccc ttctcaacga taagatagct atatttagcc
     4921 tgactttcaa aggtaattct tttgacaata tccgtactga tgttataagg aatattacca
     4981 tatatcttat agtttatatg ttttgggaag gaaaatttta gaatatccgt ttgaatcact
     5041 tttatattct cagaggggtt taccgcttct ttagtcactt gacataagcc tccatcaatt
     5101 tctatagcag taactgatcg actcattttg actagctctt tggtaaaatg tccttttcct
     5161 gatccgattt ctattacgtt gtcttgttta ctgatattcg tgtgattcaa tatttctttt
     5221 acatgctttt tagaagtaat aaaattttgc gtgtctttag ggtttttctg gttcattata
     5281 accttctcct tactggttat aatgaactga cattaagcgt tcattataac caattaattt
     5341 tgatttggtt gataacgaac tttattaata acaaatatag aaaaagtacc catactttta
     5401 tctcctttcc atttttttca ttatgaatga gataaagtaa tttctactac tgcgatactg
     5461 gtgcacataa ttgaaacctc cttatgtaac tgaatatgat tgtatcatct tttcatttta
     5521 gaataaacct ttatatgtaa ttttgcactt gacacttcag atggacgttt tcttcaaaat
     5581 aatatgtttt gaaacttgat aagagaagcc actgtcttta ctaaaaagta aaggctgcga
     5641 taaaggacag tgactttaat aaagttgcac tgttgtattg tctttggata aaattgtcta
     5701 aagggaagtt ttatccccaa cgaaaaaaca tctaaagtgg taagtaatga aagaatgtcg
     5761 ataaagtagg aatgggggat ctttttgaat acaatagaat cacagtttga taaggttgca
     5821 gaagattacg atttcgtgaa tgagcttttg aatgattatt cattctttgt gtctaatatg
     5881 tctccaaaga aaggcagagc attagatatc ggatgtggct cgggtttgtt agtggagaaa
     5941 ttagcaagtt attatgatga agtggtaggg attgatattt ctaatcaaat gctcgatctt
     6001 gccaaatcta aacgtcaact aacaaatacg gtctatctga atatgaatgc agaacaactt
     6061 aattttaatg agaagtttga ttttattgta agccgaacaa cctttcatca tttggatgat
     6121 atagccagcg taatacagca aatgaaggaa ctgttgaatg aggaaggaag aatagttatt
     6181 cttgataatg tatctgaagt cgagacaccg cctacctatg tttataaatt aggggcaatc
     6241 caagagtttt taccgcattg tttcaaattt ggaataaaaa atgcgattag aatttataat
     6301 cacaacacat ctaaatcatg gcttgagcat ttagcttcag ataagtacct atctgaacaa
     6361 aattattacg atttatatga aaagttgttg cctggatgcc aattccataa aatgggttgg
     6421 gcaatggggg ttgtctggac aaaataactt aaagctgtgg ctttgaaaag ttgaatttgt
     6481 gattgttttc agagctatcg ggtgctttag tataaataac aatgaataaa aaatcagcct
     6541 gtatcttatg ttactattaa gtaatttaag atacaggttt tactattttt gaaataagta
     6601 ccttccactt caaatacatg tattcaaagt gaaagtattg atttaaatga ttcttttgta
     6661 atttactctt gataatatga aattccatcg cagcaggata tgaattttac caaaaggata
     6721 caaaagtgtg gagaaatgat ggggatcact gtactggacc acttgatcat cggacgcaaa
     6781 cgatatttta gtttgagaga agaaggaatg atggaagaga agtaagttgc tttcttgcaa
     6841 aaaaaatgaa gcaagtgtac aattatattg taattttgtt tgggaataga agttgaaaca
     6901 tctgttgcac ctctatcgta cacgagcaat gttacaattt aaattagtgc ttttggcacg
     6961 aggaggatat tattatggaa aacggaacag taaaatggtt caacgctgaa aaaggatttg
     7021 gatttatctc acgtgaagat ggaagcgacg tattcgttca cttctcagct atccaaggtg
     7081 acggattcaa aacattagaa gaaggtcaag cagtgacttt cgacgttgaa gaatcagacc
     7141 gcggcccaca agctgcaaac gttgtaaaag cttaatttta tctgtaaatc aaattgatgg
     7201 tagacatcaa tttttacgag agaacaaagc gagtaacaaa aatacctgcc acaagagaca
     7261 atttctcttg tggcaggtat ttttttgtat gaaaaagtaa ataaatttaa gaggctatga
     7321 aagagccacc ttgtatcaag gaataagaac aaacaaaaat ggcttctcat gtttttcatt
     7381 tgttttctct tatattcagg acaaggtgct gatttcataa cctcttattt attcttcatc
     7441 tggtgtcatt accattggca aaatcattgg atgtctttct gtacgttcga ataagaaagg
     7501 ctgaagcgcg ccagtcatcg cttctttcaa tttatattct gaacagtcag gtccattcaa
     7561 acattctctt aaagcgtgga ataagattcg ctgagcttca ttgatcattt ctcctgattc
     7621 tcgcatgtag acaaatccac gagagagaat atctggacca gccaagattt cttttttctt
     7681 gatatccact gtcgcgacag ctaatacaag accttcttct gaaagcaggt gacggtcacg
     7741 taaaacgaca ttaccaatat caccgatccc gctaccatcg atatacacat ctccagccac
     7801 aaaatgccca gcaggacgcg cagaatcgct agttaacgct aaaacatcgc cattttccat
     7861 gatgaagcag ttttctgcag gaacacctac atcttgtgct agagaagcat gtactttcaa
     7921 catgcggaat tctccgtgga caggaataaa atattttggc ttcatcaaac gaagcattaa
     7981 tttttgttct tcttgtccac cgtgaccgga agtatggatg ttattgattt ttccgtgaat
     8041 cacttctgca ccagcttcag acagcaaatt gatcaaatga ttgacacttg ttgtattacc
     8101 aggaataggt gaacttgaaa agatcactgt atcgtctggc tggatttgga tttgacggtg
     8161 agtaccattc gcgatgcggc tcaacgcggc catcgcttct ccttgagaac ctgtacatag
     8221 gatcatcgtt tcattggcag gcaagcgatt gatttcgtgt gcatctacaa atgtgccgtc
     8281 tggaactttg atatatccta aacgttgacc gttgacgatg gcattttcca tgcttcgtcc
     8341 gaaaacagcg attttacgac cagttgccac agcggcatct gctgcttgct gcaaacggaa
     8401 gatgttagaa gcaaagctgg caaaaatgat acgtccttcg atttttgaaa agatcttcaa
     8461 aatagaatgc ccaatcgttt tttctgattt agtaaatgtt ggaatctcag catttgtact
     8521 atcagataaa aggcacaaga ctccttcttc cccgatacga gccattttat gcaagtttgc
     8581 tggttcgcca acaggagtga aatcgaattt gaagtctcca gtcgctacga tatttccaga
     8641 aggtgttttc accacgatcc ctaatgcatc tgggatacta tgggtcgttc tgaaaaagct
     8701 gacagaagtc tttctaaaac ggatgatcgt atcttcattg atctcatgga gctctgcatc
     8761 tcgaagcaag ccatgctcat ctaatttgtt tgtgatcaac gctaatgcga gaggaccagc
     8821 atagatagga atatttgctt gtttcagcaa gtagggtatt ccgccgatgt ggtcttcgtg
     8881 cccgtgagta atcaccaagg cttttacttt gtgtaagttt tgtatgatgt agctgtagtc
     8941 gggaatgaca tagtcaattc caagtaaatc gtcttccggg aatttgatcc cggcatcaat
     9001 caaaatgatt tcatcttgga attgtacccc atacgtattt ttcccgattt cacctaatcc
     9061 accaatagca aagacgcctg tctcgttatt tttgatggaa agtttcatat taaaactcca
     9121 ctaatgagaa atcagcattt tctttttcgt actctagatg acttccttct agaagttgaa
     9181 cgaattcaat attgtatggg gtattttctt ccacacgttg gcgagcgtct acatcgcttt
     9241 ttgcctcaat gtaaagagat tttgtttctt cccgtttagg gttgcgtact tttgtttctt
     9301 gataataaac tttatagatc attgttttgt ctcctttatc tgcgtaaagc agtgatttag
     9361 ctagttgatt tttttagaca acacaaaacg aggggacaag ccacctggtt tttcatatgc
     9421 tttgactaga atactggatt cgagtcaaag agacaagcct cgtttttgct tatctgttcg
     9481 gatatttttt caaccgtacc ttctgtataa aaaatataca acaattaatt gttattctag
     9541 cataaaacag caaataagta tagaaatcaa ctagattgaa agaaaaaatc aatgtttatc
     9601 atcccgctat aatcataatg acttttttat gagagaagtg ttctattaat aggctcgttg
     9661 tgctatcatt tttagtaatg aaaatgtaac aaaagattaa aaggagatga gaaagtgaaa
     9721 ctaatgtggc attataccat gcgttataag aaattcttgt ttttcaattt catatgcgta
     9781 tttggtttta ttttgattga attaggactt cctacgattt tagcacgtat gatcgatgta
     9841 ggaatccgca atgatgattt tgattatgtg aagcaacaag ggatatttat gatcgttatt
     9901 actgtaatcg gtattatttt gaatatcttg ttaggttatt ttggttcgag gattacgact
     9961 aatatcgtag cagatattcg agatgatttg ttcgaaaaga tccaaagtta ttcgcatcag
    10021 gaatacgaaa atctaggcgt atcttcattg atcactcgta caacaaacga tgcttatcag
    10081 atcatgttgt tcctgcaaaa tattttacgt atcggtttta tggcaccaat gatgttcatt
    10141 gtcagtttgt acatggtcat gcgtacaagt gtgacactca gtttgtatgt agtaggtgca
    10201 ttacctttac tattattggc agtcgtagcg attgctaagg tgtctgaacc gttatctaaa
    10261 aagcaacaaa aaaacttaga taaaatcaac agtatcttgc gggaaaatct ttcaggattg
    10321 cgtgtgattc gtgcattcgt caatgaaaag tttgaagaaa aacgttttgc tgtagtcaat
    10381 gagaattatg cttctagttc aaaaagctta ttccgtttga tggctgttgc acaaccgggt
    10441 ttttatttct tatttaatat cgtaatggta ttgatcatct ggagcggggc agtgcaaatc
    10501 gatgcaggcg agcttcgtgt aggagatttg attgcgttta tcgagtatat attccatgcc
    10561 ttgttctcat tcatgttatt tgctagtgtg ttcatgatgt atccaagagc agctgtttca
    10621 gctggacgta tccaagaagc cctagatgcc aaaccttcga ttgtagaaga tccaaatggt
    10681 atatctgata caaaaactaa agggtatatc gaatttcaaa atgtaacatt tgcttatcca
    10741 ggccattcgc aagagccagt gatacgaaat gtcagtttta cagcctctcc aggagagacc
    10801 gttgctttta ttggaagtac cggtagcgga aaatctacgt tgatccaatt gatccctcgc
    10861 ttttatgatg tgagtgaagg aaagatcttg atcgatggag tagacgtgag agattatcgg
    10921 ctaagcaagt tgcgagataa gattgggttt attccgcaaa aagcgctgct atttactgga
    10981 acgattgcag ataatttgcg atacgggaaa gaagatgcta cgcaagaaga aatggaacgt
    11041 gcagctgaga ttgctcaagc ttcagaattc atttctcgaa aaccagatgg atttaatgaa
    11101 catttatcag aaggtggttc caatttttca ggtggacaaa aacaacgatt ggcaatagcc
    11161 agagccatca tacgccgtcc ggaaatctat atttttgatg acagcttttc tgcattagat
    11221 tatcaaacgg atgcgaagtt acgtgctcgt ctgaaaaaag aaacgacaga atctactgta
    11281 ttgatcgtag cacaacgtgt gggaacgatc atgcatgcag ataagatcat cgtattaaat
    11341 gaaggagaag tcgtcggtat ggggacgcat cgggagttgt tggaaaaatg cccgatttat
    11401 tatgatattg ccgcttctca attatcaaag gaggaattag catgaaaaaa accttttctt
    11461 ccttaaaacg tttagctcga tatatcagac cgtatcgtga gacttttctt ttagtgatcc
    11521 tatttacagt tctgacagtg gctttcaacg tggctttgcc atatgttaca ggacttccta
    11581 ctacagaaat cagccgtaat attgccaatg gcgaatcaat caatttttct tatatattca
    11641 aatgtctcat ttggatcaca gttgttggaa tagggtattg tatttcgcaa ttactttctg
    11701 gtgttttgat gacaaatgtc gttcaaagtg ccatgcatga tttgagaaga gacatagaag
    11761 aaaaaatcaa tcgtcttccg gtttcatatt tcgataaaaa ccagcaagga aatattttgt
    11821 ctcgtgtgac gaatgatgta gatgcagtca gcggtgcttt acaacaagct tttattggta
    11881 tcgtaaatgc gatattggga attacgttgg ctgcagcaat gatgttttat atccagccga
    11941 tgatggcgct gatctctatg atcatgattc caacatcgat ctggatctca aaacgagtga
    12001 tcaatgcttc gcagaaatat tttcaaagca tgcagaattc tcttggtgag ctaaacggct
    12061 acgtccaaga aaatatgaca ggttttagtg tattgaaagt atatggacgc gaaaaagaaa
    12121 cattggaagg attcaaaaaa gttaatcaca gcttgaaata ctttgggttt cgtgcagcat
    12181 tcatttctgg cttgatgatg cctctagttc aattaactgc ctatgctact tatattggta
    12241 tggctgttct aggaagcttt tatgttatta ctggggcgat cgttgtcggt caactacaag
    12301 cattcatcca atatatttgg caaatcagtc agccaatggg gaacgtgaca caattgtctt
    12361 cagttttgca aagtgcatca gcggcaacga aacgtgtatt cgagatttta gatgagccag
    12421 aagaaaaaga aaacaaagta gatgcttcac tgccagaaac gattttagga gatgtgacat
    12481 tcgatcatgt cgattttgca tatgatccga agaaaccatt aatccaagat ttgaattttg
    12541 aagtgaaagc tggtcaaacc gtagctgtgg tcggtccgac cggtgccggc aagacgacat
    12601 tgatcaatct attgatgcgt ttttatgacg tagacaaagg agcaataagg attgatggaa
    12661 tcgacaccaa atcaatgagt cgcagtgatg tgcgttcgct atttggtatg gtcttgcaag
    12721 atgcatggct ttatcaagga acgattgcag ataatatccg cttcggaaag cttgatgcga
    12781 cagattatga agtagttgat gctgcgaaaa cagcaaacgt agatcatttt atccgaacga
    12841 tgccagatgg atatgatatg gccatcaatt ctgagggaga caatgtgtct ttaggacaaa
    12901 aacagttgct gacgattgcc cgagcagtgg tctccgatcc taaaatcttg attttagatg
    12961 aagcaacaag ttcagtagac acccgtttag aagcgttgat tcaaaaagca atggatcgcg
    13021 tgatggaggg acggacaagt ttcgtcatcg ctcaccgttt atcgacaatc cgtgatgcag
    13081 atctgattct agtgatggat caaggtcaga tcattgaaaa aggaacgcat gagagtttat
    13141 tggcacaagg tggattttat gaaaaactgt ataacagcca atttgctgag gaagcagaat
    13201 aagtcatata agaataaaac agtttaaaac ttattttttg ttctctattc ttgataatgg
    13261 taaagtaaag ttatcaagaa aagtgaggac acggagatgg aacagttttt aaatgaaaaa
    13321 attactgaag ccaaaagaga agttgatcgt ttaac
//
LOCUS       DAPCMT010000362        13213 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.guided.080, whole genome shotgun sequence.
ACCESSION   DAPCMT010000362 DAPCMT010000000
VERSION     DAPCMT010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.guided.080"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(204..2162)
                     /locus_tag="RD743_003029"
     CDS             complement(204..2162)
                     /locus_tag="RD743_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0564768.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="RD743_003030"
     CDS             complement(2159..3031)
                     /locus_tag="RD743_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0564769.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="RD743_003031"
     CDS             complement(3373..4245)
                     /locus_tag="RD743_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0564770.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="RD743_003032"
     CDS             4373..4942
                     /locus_tag="RD743_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDU0564771.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="RD743_003033"
     CDS             5009..5746
                     /locus_tag="RD743_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0564772.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="RD743_003034"
     CDS             5977..7245
                     /locus_tag="RD743_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDU0564773.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="RD743_003035"
     CDS             7732..8196
                     /locus_tag="RD743_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0564774.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="RD743_003036"
     CDS             8180..8809
                     /locus_tag="RD743_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDU0564775.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="RD743_003037"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="RD743_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDU0564776.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="RD743_003038"
     CDS             10688..11134
                     /locus_tag="RD743_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDU0564777.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="RD743_003039"
     CDS             complement(11333..11848)
                     /locus_tag="RD743_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDU0564778.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="RD743_003040"
     CDS             11965..13167
                     /locus_tag="RD743_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0564779.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAPCMT010000363         8061 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.guided.124, whole genome shotgun sequence.
ACCESSION   DAPCMT010000363 DAPCMT010000000
VERSION     DAPCMT010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.guided.124"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            128..1564
                     /locus_tag="RD743_003041"
     CDS             128..1564
                     /locus_tag="RD743_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0564780.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="RD743_003042"
     CDS             1753..4200
                     /locus_tag="RD743_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDU0564781.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="RD743_003043"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="RD743_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDU0564782.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="RD743_003044"
     CDS             complement(5690..6793)
                     /locus_tag="RD743_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDU0564783.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="RD743_003045"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="RD743_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDU0564784.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2696 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DAPCMT010000364         4696 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.guided.174, whole genome shotgun sequence.
ACCESSION   DAPCMT010000364 DAPCMT010000000
VERSION     DAPCMT010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.guided.174"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(73..1014)
                     /locus_tag="RD743_003046"
     CDS             complement(73..1014)
                     /locus_tag="RD743_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDU0564785.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1140..2261
                     /locus_tag="RD743_003047"
     CDS             1140..2261
                     /locus_tag="RD743_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDU0564786.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2519..4021
                     /gene="eat(A)"
                     /locus_tag="RD743_003048"
     CDS             2519..4021
                     /gene="eat(A)"
                     /locus_tag="RD743_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDU0564787.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4049..4615
                     /locus_tag="RD743_003049"
     CDS             4049..4615
                     /locus_tag="RD743_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDU0564788.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1520 a          848 c         1006 g         1322 t
ORIGIN      
        1 tttttgatct tttattgggc ttaaaaaaag agaaccagct gctgattcag ctgattctca
       61 tcagagagcc ttttattctg cgccaaggat tccttctttt gcatggacta attgggtcaa
      121 aaaggcacaa tatggtgtag caatatcata tttttgtcct tttcgccaaa tggcaccatt
      181 gatgtagtcg atttcagtca aacgatggtt tttgatcagg tcttggtaca tagatgggta
      241 atgcaacccg atgttgtcag gatcataagt agaagcaata tgttggtaca cttcttcttg
      301 atctagtatg attccttctt ttgctgcgat agaagcaaat tcagatacga ttgttcgaac
      361 catgctctct gccgcttttt gcgcgcctag ttcagcaata ttacaatcca aaatcgtgca
      421 taaaccattc aatgttccat ttacacaagc ttttcgccaa atggaataac gaacgttatg
      481 gctatagatc ggatttaaat ttgcttcttg aaagacttct acaacttttt ttgtaaatgc
      541 ttcgccttct ggttccaagt tctctagttc gatttctcca tcacctaaca atgtgacttt
      601 tccgggacca gctagaccag ctgtccacat cgtgatccca aataagatat ttttctttgg
      661 cacatatttt tccagtacat cttcatgacc taatccattt agtaaacaca aaacataagt
      721 attgtcagta ataatggatt ggatcgaaca aaacatatca tctaattgat tggcttttgt
      781 taatgctacg atcaagtcca cgtgttcgtt gctttcgatg atttcttctg gtgaatagat
      841 tggcagtttt gcgaccactt ctttaccgtt aaagtcagca atcaacccat ttgtacgaat
      901 ggcttcaatg tgagcaggcc aacgatcgat caagaggacc tcatttccgc tttgatgtag
      961 catcaatccg attcggctgc ccattgctcc ggccccagct attgcgattt tcatgttttt
     1021 tctctccttt attcaatgag ttccctttta gtatatagaa aaatggaaga aaatagcatt
     1081 gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag aagaaagtga gggatacaga
     1141 tggatatttt ggcggcaatt gattcattta aaggaagtgc ttcttcattt gaactaaatc
     1201 aggcagttct aaatggctgg gaagatcgta aaggaagaaa aatcaatgtg ccgattgcag
     1261 atggcggaga aaatactgca gaagcaatct attacgcgct caggggaaca tggcgtacca
     1321 ttgatgggat ggatctctta ttccgtcagc gaaaaatcag ctatttattg acaacttggc
     1381 aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt ggggttagat ctacttgata
     1441 aaccaacgga tcaaactatc cgacttgctt ccagttatgc cttaggagaa cttattaaag
     1501 atgcactgct tcaagaagta gaacaaatta ttgtcacttt aggaggaagc gggtgtagcg
     1561 atggtggtct aggattactc caatccttgg gtgcttcttt agaagggtac acagaaggaa
     1621 atcctttatt atctactaaa aaacttcatt ttgatcgaag aaacgttctt gtttctaaag
     1681 taaaattgat tatcgctgca gatgttacca atccatatac aggtcaacaa ggagctgctt
     1741 ggcaatttgg aagacaaaaa ggaggaatag atacgacgct agctttctta gataaacaag
     1801 caggaaagat tgctcagcaa ataaaagatt tctgggaggt ggatttagat gatattccag
     1861 gaagtggagc agcaggcggt attggtggag cccttttttt gttgggagcg aagatggttt
     1921 ctggtttcga tttagtcagc caaatcactg gattggaaaa aaagatcgaa caagcagatg
     1981 tcatcattac aggagaaggg cggatcgatc agcaaacgat ccacggaaaa gttccatacg
     2041 gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc ggaagtagag
     2101 accaagagct agaaggactt tctgattttc tcccaattgt cttaagtatt catttgggtc
     2161 caataggttt agaagaagca atggaacaca ggcaaacatt agaaaaaaca aaaatcttag
     2221 gacagacact tagcagattt ttcaccatcc tcccgaagta gttgacaaaa tcattttctt
     2281 tatgctatcg taatttcaaa tataggacaa agtgagggat ccgtgcattg aaaaattaag
     2341 tcgattgatg aaagctgcat tcagactgta ccttgtggag acaaggaagc agtgtgcaga
     2401 ttttcgggat tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat ccaacccttg
     2461 catcctcttt agccagttct gaaaatagga ctggtctttt tttacgaggt gatggaaaat
     2521 gtctaaaatc gaaataaaaa atctgacatt cggctacgac agccaaggca cattattatt
     2581 tgaacaagca aatctaaatt ttgacacaca atggaaacta ggacttatcg gacgaaacgg
     2641 tcgaggaaag acaactttac tgaatattct acaaaacaaa ctaccttatc aagggcaagt
     2701 aatccatcag caagaatttg cctatttccc gcaacagaca aaagataaag aacgtttaac
     2761 ctattacgtg ttaaatgata ttacggattt tgagatatgg gaaatcgaaa gagagctcca
     2821 attgatgcaa acagatcctg aaatcttatg gagagaattc agcacactat cggggggaga
     2881 gaagacaaaa gtcctactgg cacttttatt tgtggatgac actcatttcc cgttaatcga
     2941 tgaaccaacg aatcatttgg atatctctgg tagaaaacaa gtagcggctt atttgaaaaa
     3001 gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga tttatcgatg aagtagtgga
     3061 ccatgtttta gcaatcgaaa aaagtcaact ggaactttat caagggaatt tctctatcta
     3121 tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa
     3181 agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga
     3241 aggagataaa acaaagaaac aagtcggatt catcgatact gaatctagac gagtgaataa
     3301 aggagcagtg ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg
     3361 gatggagacc cagatcagcg agaaagaaaa actattaaaa gatatcgaat atatcgattc
     3421 tttgacgatg aatagccaag cgtctcacca taagcgactt ttaagcgtag aagatcttca
     3481 attagggtat gaaaatctgt tattcgagcc aattcatttt acaatcgagc ctcatcagcg
     3541 ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc attatccatt atcttctggg
     3601 ggcattcaac ggcaaggtta taggagaaaa ataccagcca aaacatctga gcattagtta
     3661 tgcaagccaa aattatgaag acaatcgagg aacgttggcg gaatttgcag agaaaaacca
     3721 agtagactac caagcatttt tgaacaacct ccgaaagctt gggatggaaa gagatgtttt
     3781 tcataacaag atcgagcaga tgagtatggg ccaacggaaa aaagtggaat tggctaaatc
     3841 tttatcacag ccagctgaac tatatacatg ggatgaacca ttgaattatt tggatgtctt
     3901 caatcaagaa caattagaac aactgatctt gaacgtgaaa cctgccatgt tactagtgga
     3961 acatgatcaa accttcctgg ataaagtatc tactgagatt atttctcttg agagaatcta
     4021 acccttgtaa tctcaagaac aacaaggtat ggttaagggg aactttgcca acgaaggaga
     4081 gaatcacatg ctttatgtaa cgagacacgg agaaacaact tggaacgcac aaggattagt
     4141 ttgcggacgg gcagatgtgc cgctgactga aaaaggacag atgcaggcac agaaactagc
     4201 agaaaaggta gtagatttgc ctgttccaat cacgaaaatc atccattccc ctcttcaacg
     4261 agcaagagat acagcacaag cagttgctga tcgtctcagc ttgccgctga ccgttgatga
     4321 acgattggta gaaatggatt ttggggatta tgatggactg ccaagcaaag atgagaactt
     4381 tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat ggggagtcag tcttagatgt
     4441 gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa gacgaagaaa atgtctatct
     4501 gttagtttgc cataatgcct tgataagagt gatcaatgct tattttcatc cgatgcctaa
     4561 tgaggggttc tttacattca tggtagataa tacagaattg gtttcttatg agtaattatg
     4621 tttttgtgtt atttaaataa aatatttcaa ggcactgctt ttgcttagca gtgccttttt
     4681 taggttatga caattc
//
LOCUS       DAPCMT010000365         3356 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.guided.198, whole genome shotgun sequence.
ACCESSION   DAPCMT010000365 DAPCMT010000000
VERSION     DAPCMT010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.guided.198"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            315..812
                     /gene="dfrG"
                     /locus_tag="RD743_003050"
     CDS             315..812
                     /gene="dfrG"
                     /locus_tag="RD743_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDU0564789.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(884..2836)
                     /locus_tag="RD743_003051"
     CDS             complement(884..2836)
                     /locus_tag="RD743_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDU0564790.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2843..3079)
                     /locus_tag="RD743_003052"
     CDS             complement(2843..3079)
                     /locus_tag="RD743_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564791.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1039 a          576 c          552 g         1189 t
ORIGIN      
        1 gtaaatgtag tactccacta taaaggcgta tatatgcaag taagtagttc agttttggag
       61 tacaaaatga caaaatgtac ttcaaaatgg actacttttt tcattttaac ttacatacca
      121 aattacatac aaaatgattt ataaaatgca attgaaaatg ataattgaat gaaaatttgt
      181 gattttgtgg tattatgata atataactta tagaaaaaat tgcttattca actaacgggc
      241 aggataatga gaaaaggttt tttgatagaa ataagaaatt agatggtaaa ttagttaaaa
      301 aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat ggataagaat agagtgattg
      361 gcaaagagaa tgacattcct tggaggattc ccaaggactg ggaatatgtt aaaaatacta
      421 caaagggaca tccgataata ttaggtagga agaaccttga atcaatcgga agagccttac
      481 ctgacagaag aaatattatt ctgacgagag ataaggggtt tacctttaat ggttgtgaaa
      541 ttgttcattc aatagaagat gtttttgagt tatgtaaaaa cgaagaagaa atttttattt
      601 tcggaggaga acagatttat aatttgtttt tcccttatgt tgagaaaatg tacatcacaa
      661 aaatacatca tgaattcgaa ggagatactt tttttccaga agtgaattat gaggaatgga
      721 atgaggtatt tgcccaaaaa gggataaaga atgataaaaa tccgtataac tactattttc
      781 atgtatatga aagaaaaaac ttattgagtt aacgggtgcg ttaattgaag aagaacagta
      841 aagataaagc acaagagctt gggattactc caagctcttt ttgtcatttg aaataaatta
      901 tatctttcca atcaaattgt ttatctgtaa tccctaatct ttgagcaggt gtcaaaacct
      961 ttttatccgc tgatttaaaa gggcgacaaa agttgtagta agttcttaga attgtaatcg
     1021 caaattgagc atacttcgga ttgaagttag cgtaaatata gctcttctta tcaccacgag
     1081 ctgttgttaa tggtctttca agagatgata tataacgtct tatctgttga ataaagctat
     1141 tggtcgaatg gtcattaaca tttaatagca tctttgcgat ttcgtttggt tccagagcag
     1201 ataaatccgt tctacaatca actgaataaa atcctttatc cttagtggct aatggatgtt
     1261 tgatagggtt atcggcatat ttgcgatatc tttctccatc tttactaata gcctcttcgt
     1321 ggaattgatg acttgttagt aattcagtta gataatgaga tgccaaagtc cgtagaaact
     1381 ttgttttgaa tcctctgata tctccccaat cgagcagttc tgcttttgct tgtccgaatt
     1441 cttttaagca ttgttttctg cttttcgtct tgttaacttg gcaaataaaa tgatgtgcat
     1501 cgctcaacct taattctttc gtgaatactc tataaaaggc gtttctaata gagaagtcat
     1561 catcactaat cattcgccat tcgtcggcat taaccatttg tttaattaac caatagtgag
     1621 cagttgtagt ataaggagca tcaacattta aaccatcgac aaatctactc ctattagtaa
     1681 ttttccctaa ttcgtgaaga taagcattcc tattttcgct atcgttagcg gatggttctt
     1741 gtggataata agaccaatct aacctatcat ttttacgact aaaagtattg agatggtctt
     1801 ctttgaattt tcttgtatct tcatttaatt catccattga aatattccaa tcataggcaa
     1861 catcagaacg gaacacatat cttgataaga cctcagcaga tacaactata tacgtttgca
     1921 tattaaggtc ttcaaacccc gtatatttct ttgaaccttg tcctttctta cggacgttat
     1981 ttaggtagta gtgcatcttg tccgtattaa gccacatctc gttaaatttc tttgtttgaa
     2041 ggggttgtgt ctcatatcgt tctaaaaact ccaaacaacg cctatacagc cactccagct
     2101 tatgatagta ggttccaact ccaattccta aaatatcaca agtacgactg actgaaaccc
     2161 ttccaacaac catcttagcg aacattggaa gaatggtgtt cttttgttgg tgataagtag
     2221 tggtttcttc tctttttggt agaacattag tgaacttctt acacgcctta cattgatagc
     2281 gttgagattt gcccctactt ttcccccttt tgtaaaattg cttcggctca ttgaatgggg
     2341 ttgattcttc ttccgagcag ccttctttat gaaattgata atctggctca acatcttgga
     2401 tgctattgat ttctataagg cgtttaattt cttcaacaac agaccaatta gataaagggg
     2461 ttactgtaca gttttgcgat acacctctgc caataggatt gtcattgcag tacatacctt
     2521 tatgaccttt atctttggaa ctaccagtca ttttatatcg acttggtttc ccttttacat
     2581 ctttgaattt gtgctgttct ttcccaaaat tacaacaaaa cggattgatg cagtaattat
     2641 attgaatggg atattcagaa ccgttccatt taaaagacgt tggcaagaac aacattttgt
     2701 tataccttgt tgcaaatcgt tttggagtta ataaatcata ttgtttagcc ctatctttta
     2761 attcttcgtt tgaaatcgga attttaacat ctacaatttc atcttccttc gtggctaatc
     2821 ttttaagctt ggtcattgtc tatcaaccca ctctatcttt gatacccatt ttttcaacaa
     2881 tccgcttatt acttcgcttg ttggcaatcc attcaataaa cttcttatcg tctaaaatgt
     2941 tcaataagaa ttcatccact gcttcgcttt cagtacgctc tgcatattca gcgtattgct
     3001 taactatcac ccttacttgc tctgatattt cccaatccac agacttagcg ttcttatttt
     3061 ttggctcaag aaattgcata ggaaaatgca cccctttttg cttagtaaat tgtaaccatt
     3121 ttaacacacc aatttgcatt cctacaataa ttatttcacg ttcgcatttt tatatacatt
     3181 ttaacttaca ttttgtggtg taaattaaca acaaacgttg atttaacaat aaaaaacgag
     3241 tatcaaattc agttgcaatt tgatactcat ttaggtttac attttcactt tttgtactcc
     3301 aaaactgaac tacttaatat gcaagacgtt gtcttttttt tgtatattca attttg
//
LOCUS       DAPCMT010000366         3134 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.guided.201, whole genome shotgun sequence.
ACCESSION   DAPCMT010000366 DAPCMT010000000
VERSION     DAPCMT010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.guided.201"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(40..1026)
                     /locus_tag="RD743_003053"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="RD743_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="RD743_003054"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="RD743_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDU0564792.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="RD743_003055"
     CDS             1851..3005
                     /locus_tag="RD743_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDU0564793.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          967 a          615 c          644 g          908 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg tttt
//
LOCUS       DAPCMT010000367         2877 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.guided.209, whole genome shotgun sequence.
ACCESSION   DAPCMT010000367 DAPCMT010000000
VERSION     DAPCMT010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.guided.209"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            126..1094
                     /locus_tag="RD743_003056"
     CDS             126..1094
                     /locus_tag="RD743_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDU0564794.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1087..2118
                     /gene="vanA"
                     /locus_tag="RD743_003057"
     CDS             1087..2118
                     /gene="vanA"
                     /locus_tag="RD743_003057"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDU0564795.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2124..2732
                     /locus_tag="RD743_003058"
     CDS             2124..2732
                     /locus_tag="RD743_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDU0564796.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          851 a          554 c          705 g          767 t
ORIGIN      
        1 cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag agaaagattt
       61 gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct
      121 taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat
      181 tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg
      241 aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga
      301 tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa
      361 gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca
      421 atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag
      481 tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg
      541 accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca
      601 aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc
      661 gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta
      721 cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga
      781 gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg
      841 agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag
      901 gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac
      961 ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc
     1021 aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac
     1081 aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac
     1141 gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa atacgagccg
     1201 ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa
     1261 tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat gcacggatta
     1321 cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt ttcagctttg
     1381 catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc cggtatccct
     1441 tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt gacatacatc
     1501 gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa agatgatagg
     1561 ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc
     1621 ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga atcggcaaga
     1681 caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg
     1741 gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg accaaatcag gctgcagtac
     1801 ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata
     1861 accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa
     1921 atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt acaagataac
     1981 ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata cagtcgttat
     2041 ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg cttgatcgta
     2101 ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat gaaatagtac
     2161 acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga aaaccggttg
     2221 acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg cttttgaagg
     2281 caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt taccgtccta
     2341 agcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg
     2401 aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac gtggcttcaa
     2461 aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta gacacgggtg
     2521 agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat catgcggcaa
     2581 atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca
     2641 gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac gaaccatacc
     2701 ccaatagcta ttttgatttc cccgttaaat aaacttttaa ccgttgcacg gacaaactat
     2761 ataagctaac tctttcggca ggaaacccga cgtatgtaac tggttcttag ggaatttata
     2821 tatagtagat agtattgaag gttctgttgc aaagttttaa ataaagaata aaatccc
//
LOCUS       DAPCMT010000368         2440 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.guided.224, whole genome shotgun sequence.
ACCESSION   DAPCMT010000368 DAPCMT010000000
VERSION     DAPCMT010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.guided.224"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..75
                     /locus_tag="RD743_003059"
     CDS             <1..75
                     /locus_tag="RD743_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDU0564797.1"
                     /translation="PMVMHKNPSDKSIYHWDFYALLGF"
     gene            91..2010
                     /gene="tet(M)"
                     /locus_tag="RD743_003060"
     CDS             91..2010
                     /gene="tet(M)"
                     /locus_tag="RD743_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDU0564798.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGIVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2129..2296
                     /locus_tag="RD743_003061"
     CDS             2129..2296
                     /locus_tag="RD743_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDU0564799.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2356..>2440)
                     /locus_tag="RD743_003062"
     CDS             complement(2356..>2440)
                     /locus_tag="RD743_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDU0564800.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          837 a          370 c          488 g          745 t
ORIGIN      
        1 cctatggtta tgcataaaaa tcccagtgat aaaagtattt atcactggga tttttatgcc
       61 cttttgggtt tttgaatgga ggaaaatcac atgaaaatta ttaatattgg agttttagct
      121 catgttgatg caggaaaaac taccttaaca gaaagcttat tatataacag tggagcgatt
      181 acagaattag gaagcgtgga caaaggtaca acgaggacgg ataatacgct tttagaacgt
      241 cagagaggaa ttacaattca gacaggaata acctcttttc agtgggaaaa tacgaaggtg
      301 aacatcatag acacgccagg acatatggat ttcttagcag aagtatatcg ttcattatca
      361 gttttagatg gggcaattct actgatttct gcaaaagatg gcgtacaagc acaaactcgt
      421 atattatttc atgcacttag gaaaatgggg attcccacaa tcttttttat caataagatt
      481 gaccaaaatg gaattgattt atcaacggtt tatcaggata ttaaagagaa actttctgcc
      541 gaaattgtaa tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac gaactttacc
      601 gaatctgaac aatgggatac ggtaatagag ggaaacgatg accttttaga gaaatatatg
      661 tccggtaaat cattagaagc attggaactc gaacaagagg aaagcataag atttcagaat
      721 tgttctctgt tccctcttta tcatggaagt gcaaaaagta atatagggat tgataacctt
      781 atagaagtta ttactaataa attttattca tcaacacatc gaggtccgtc tgaactttgc
      841 ggaattgttt tcaaaattga atatacaaaa aaaagacaac gtcttgcata tatacgcctt
      901 tatagtggag tactacattt acgagattcg gttagagtat cagaaaaaga aaaaataaaa
      961 gttacagaaa tgtatacttc aataaatggt gaattatgta agattgatag agcttattct
     1021 ggagaaattg ttattttgca aaatgagttt ttgaagttaa atagtgttct tggagataca
     1081 aaactattgc cacagagaaa aaagattgaa aatccgcacc ctctactaca aacaactgtt
     1141 gaaccgagta aacctgaaca gagagaaatg ttgcttgatg cccttttgga aatctcagat
     1201 agtgatccgc ttctacgata ttacgtggat tctacgacac atgaaattat actttctttc
     1261 ttagggaaag tacaaatgga agtgattagt gcactgttgc aagaaaagta tcatgtggag
     1321 atagaactaa aagagcctac agtcatttat atggagagac cgttaaaaaa tgcagaatat
     1381 accattcaca tcgaagtgcc gccaaatcct ttctgggctt ccattggttt atctgtatca
     1441 ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg tttctcttgg atacttaaat
     1501 caatcgtttc aaaatgcagt tatggagggg atacgctatg gctgtgaaca aggattgtat
     1561 ggttggaatg tgacggactg taaaatctgt tttaagtatg gcttatacta tagccctgtt
     1621 agtaccccag cagattttcg gatgcttgct cctattgtat tggaacaagt cttaaaaaaa
     1681 gctggaacag aattgttaga gccatatctt agttttaaaa tttatgcgcc acaggaatat
     1741 ctttcacgag catacaacga tgctcctaaa tattgtgcga acatcgtaga cactcaattg
     1801 aaaaataatg aggtcattct tagtggagaa atccctgctc ggtgtattca agaatatcgt
     1861 agtgatttaa ctttctttac aaatggacgt agtgtttgtt taacagagtt aaaagggtac
     1921 catgttacta ccggtgaacc tgtttgccag ccccgtcgtc caaatagtcg gatagataaa
     1981 gtacgatata tgttcaataa aataacttag tgtattttat gttgttatat aaatatggtt
     2041 tcttgttaaa taagatgaaa tattttttaa taaagatttg aattaaagtg taaaggagga
     2101 gatagttatt ataaactaca agtggatatt gtgtcctgta tgtggaaata aaacacgatt
     2161 aaagataagg gaagatactg aattaaaaaa attccccctc tattgtccga aatgcagaca
     2221 agaaaattta attgaaataa agcagttcaa agtaactgtg attacagagc cagacgcaaa
     2281 gacgcagagc cgataaaatg agattaatac aatctcattt tatcggctct ttccgttatg
     2341 tatggattct tttaattagt cttcgatgtt tcttgcttcg ttgataccgc tggctaaaga
     2401 ttccattaag gatagttctt tgtctgtaaa gctatccatg
//
LOCUS       DAPCMT010000369         2438 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.guided.225, whole genome shotgun sequence.
ACCESSION   DAPCMT010000369 DAPCMT010000000
VERSION     DAPCMT010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.guided.225"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..75
                     /locus_tag="RD743_003063"
     CDS             <1..75
                     /locus_tag="RD743_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDU0564801.1"
                     /translation="PMVMHKNPSDKSIYHWDFYALLGF"
     gene            91..2010
                     /gene="tet(M)"
                     /locus_tag="RD743_003064"
     CDS             91..2010
                     /gene="tet(M)"
                     /locus_tag="RD743_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDU0564802.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGIVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2108..2296
                     /locus_tag="RD743_003065"
     CDS             2108..2296
                     /locus_tag="RD743_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDU0564803.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2354..>2438)
                     /locus_tag="RD743_003066"
     CDS             complement(2354..>2438)
                     /locus_tag="RD743_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDU0564804.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          838 a          372 c          473 g          755 t
ORIGIN      
        1 cctatggtta tgcataaaaa tcccagtgat aaaagtattt atcactggga tttttatgcc
       61 cttttgggtt tttgaatgga ggaaaatcac atgaaaatta ttaatattgg agttttagct
      121 catgttgatg caggaaaaac taccttaaca gaaagcttat tatataacag tggagcgatt
      181 acagaattag gaagcgtgga caaaggtaca acgaggacgg ataatacgct tttagaacgt
      241 cagagaggaa ttacaattca gacaggaata acctcttttc agtgggaaaa tacgaaggtg
      301 aacatcatag acacgccagg acatatggat ttcttagcag aagtatatcg ttcattatca
      361 gttttagatg gggcaattct actgatttct gcaaaagatg gcgtacaagc acaaactcgt
      421 atattatttc atgcacttag gaaaatgggg attcccacaa tcttttttat caataagatt
      481 gaccaaaatg gaattgattt atcaacggtt tatcaggata ttaaagagaa actttctgcc
      541 gaaattgtaa tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac gaactttacc
      601 gaatctgaac aatgggatac ggtaatagag ggaaacgatg accttttaga gaaatatatg
      661 tccggtaaat cattagaagc attggaactc gaacaagagg aaagcataag atttcagaat
      721 tgttctctgt tccctcttta tcatggaagt gcaaaaagta atatagggat tgataacctt
      781 atagaagtta ttactaataa attttattca tcaacacatc gaggtccgtc tgaactttgc
      841 ggaattgttt tcaaaattga atatacaaaa aaaagacaac gtcttgcata tatacgcctt
      901 tatagtggag tactacattt acgagattcg gttagagtat cagaaaaaga aaaaataaaa
      961 gttacagaaa tgtatacttc aataaatggt gaattatgta agattgatag agcttattct
     1021 ggagaaattg ttattttgca aaatgagttt ttgaagttaa atagtgttct tggagataca
     1081 aaactattgc cacagagaaa aaagattgaa aatccgcacc ctctactaca aacaactgtt
     1141 gaaccgagta aacctgaaca gagagaaatg ttgcttgatg cccttttgga aatctcagat
     1201 agtgatccgc ttctacgata ttacgtggat tctacgacac atgaaattat actttctttc
     1261 ttagggaaag tacaaatgga agtgattagt gcactgttgc aagaaaagta tcatgtggag
     1321 atagaactaa aagagcctac agtcatttat atggagagac cgttaaaaaa tgcagaatat
     1381 accattcaca tcgaagtgcc gccaaatcct ttctgggctt ccattggttt atctgtatca
     1441 ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg tttctcttgg atacttaaat
     1501 caatcatttc aaaatgcagt tatggaaggg atacgctatg gttgcgaaca aggattatat
     1561 ggttggaatg tgacggattg taaaatctgt tttaagtacg gtttatacta tagccctgtt
     1621 agtactccag cagattttcg gatgcttact cctattgtac tggagcaagc ctttagaaaa
     1681 gctggaacag aattgttaga gccatatctt agttttaaag tttatgcacc acaggaatat
     1741 ctttcacggg catataacga tgctcccaaa tattgtgcaa atatcgtaaa tactcaactg
     1801 aaaaataatg aggtcattat tattggagaa attcctgctc gatgtattca agattatcgc
     1861 aatgatttaa ctttttttac aaatgggctt agtgtttgtt tagcagagct aaaaggatat
     1921 caggttacca ctggcgaacc tgtttgccag acccgtcgtc taaatagtcg gatagataaa
     1981 gtaagatata tgttcaataa aataacttag tgcgttttat gttgttatat aaatatggtt
     2041 tcttattaaa taagatgaaa tattctttaa tatagatttg aattaaagtg gaaaggagga
     2101 gattgttatt ataaactaca agtggatatt gtgtcctatt tgtggaaata aaacaagact
     2161 acgaatacga gtggatacta tacttaaaaa tttcccttta tactgcccca aatgtaagaa
     2221 cgaaacttta attaatgttc aaaaaatgaa tataataaca atcaaagagc cagacgccaa
     2281 gacgcagagc cgataatttg agaaatgaaa ctctcatctt atcggctctt tttgtttatc
     2341 tgaattttac tgactagcct tcaatatttc ttgcttcatt gatacctttt gctagtgctt
     2401 ccattaagga tagttctttg tctgtaaagc tatccatg
//
LOCUS       DAPCMT010000370         2171 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.guided.235, whole genome shotgun sequence.
ACCESSION   DAPCMT010000370 DAPCMT010000000
VERSION     DAPCMT010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.guided.235"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(660..950)
                     /locus_tag="RD743_003067"
     CDS             complement(660..950)
                     /locus_tag="RD743_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0564805.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1158..1850)
                     /locus_tag="RD743_003068"
     CDS             complement(1158..1850)
                     /locus_tag="RD743_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDU0564806.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          620 a          401 c          319 g          831 t
ORIGIN      
        1 cttaaagata acttaaaagt tatcttttaa atacttaatt gttaaatacc ttatttgatg
       61 caaaagaaat ctacgatagt caacaaaaaa attttcatta tatccagaaa aatttttctt
      121 agttaaggac ctatggattg cagtaatgca gaattcgtcg aataaactgc caaagacata
      181 caatcatatt gcttttaaaa ttgacgaaca agatatagat tcgtttgtct caaaaatcca
      241 aatgttaggt ttaactgtcg aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat
      301 ttacttttac gactatgata atcacctctt cgagttacat accggtactc tccaggaacg
      361 tttaaaatcc tacaattcca ctacctgaga ctagtaatga ctagtctctt tttatggcct
      421 ctgatccccc cattgagcga atactaatag ggcttcgaat tgatctgttc aatctttaag
      481 caatatccct caaatgagtt tggaaagttt agtgattcat cacaaatact taaatacatg
      541 tagttatata tagattgagg ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg
      601 tctgcatcca tctttccaaa acaagcggct aggacatttt tgtctcagcc gcttgttttc
      661 tagtttattc tttcatcttg tcggaccttt tttcttgggt atagactttt ctcatattct
      721 accacaattt tgggtttccc taacacatga taggatctca caacaaattg ctttctatta
      781 ttataataac ctgtaacgac taacttcatc ccttcgtcta cttcagctag gaagttcaaa
      841 ctatgggaag caatcaaaca attgacccca tttaaactga aacggatcaa cggtctggca
      901 ctaacttgca aagtacggat cttatctact gttccatatt ttttctccat aaccttcacc
      961 tcaataccat tatacgaaca tacattccct ttgtaaagaa gaaactctgt tttatctttt
     1021 tgactttttg tctattacct ttcttaaaat catattggca cttcctgcga agatgaagga
     1081 catgtactaa aaaaaagagg cagcagtata gattactgtt gcttctttac taatttaggt
     1141 ttacttgtta agaaagatta tttacgcaaa tgttcttcaa caatttgctg agatttatca
     1201 gctgtatctt ccacttcatc ttttgctttt tctaactttt gaatatattg tttcaagctt
     1261 gagttttcag cagttgcttc gtctaatttc ttttgtgtat ctgttaactg agtttctaca
     1321 tttgtttttt cttgtgtcag tgttgcgatt tgttgattca aaccatttac agtattttgt
     1381 aattctgaaa gttttgcttg tgcttcttgt agtcttgctg tccaatctgc attcagatga
     1441 ttgatttcat catttttttg gttgatctca tctagtttga cattgatttc attttgtttg
     1501 ctttgtagtt gtgcatcctt gctgactaat tgactttcta agttttgttt ttctgctgcc
     1561 gattgatttt tttggttttc taattcattt acttttgttt ggtattcttg gacatctgcc
     1621 tttgaatctt gcaatgtctg ttctttttgt gctagtcgag cggtaagatc tgtaagcgct
     1681 tgctctttat ctgtcaatgt atgatcaagc tgtgtcagat tctcattgat tgtatcaata
     1741 tctgtctctc caccccacaa aataatttca tttgcgtacc gcgtgcctgt gtagccaatt
     1801 agacatagaa atgcaacgac aatcccacca gtaataaatt tctttttcat ttgcatagct
     1861 ccttaatcca atacttttac atgctaaaag taagtgatct caaacacaat gtcaagcgtt
     1921 tttaaattac atgtgtaatt ttaatataaa tactatcaaa tactcattta atcctccttt
     1981 tttatctatt taaaaaatct tttttataga gaaaaatgca ggttaatact ttcttaaata
     2041 aatatttatg gtgaacaaaa acaaaaaagc aagttttatt aaacgtaacc cttttcattt
     2101 catagattac tgtatttatt acaaaaaacg cggatttgtc ataaattgtc atgcttcttt
     2161 tacctatgaa a
//
LOCUS       DAPCMT010000371         1350 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.guided.280, whole genome shotgun sequence.
ACCESSION   DAPCMT010000371 DAPCMT010000000
VERSION     DAPCMT010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.guided.280"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            47..298
                     /locus_tag="RD743_003069"
                     /pseudo
     CDS             47..298
                     /locus_tag="RD743_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="RD743_003070"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="RD743_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDU0564807.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAPCMT010000372         1133 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076051
            SAMN33619550-rid17849423.guided.294, whole genome shotgun sequence.
ACCESSION   DAPCMT010000372 DAPCMT010000000
VERSION     DAPCMT010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619550
            Sequence Read Archive: SRR23719554
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710970.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710970.1
            Genome Coverage       :: 200x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 09:07:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 21 of 66
            Pseudo Genes (incomplete)         :: 34 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 5 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619550-rid17849423.guided.294"
                     /strain="AUSMDU00076051"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            23..106
                     /locus_tag="RD743_003071"
     CDS             23..106
                     /locus_tag="RD743_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDU0564808.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            231..968
                     /gene="erm(B)"
                     /locus_tag="RD743_003072"
     CDS             231..968
                     /gene="erm(B)"
                     /locus_tag="RD743_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDU0564809.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          431 a          186 c          173 g          343 t
ORIGIN      
        1 aattaagaag gagggattcg tcatgttggt attccaaatg cgttatgtag ataaaacatc
       61 tactgttttg aaacagacta aaaacagtga ttacgcagat aaataaatac gttagattaa
      121 ttcctaccag tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc
      181 gtttaacttc tgtatttgtt tatagatgta atcacttcag gagtgataac atgaacaaaa
      241 atataaaata ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac
      301 aattgaattt aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa
      361 cgacgaaact ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat
      421 tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata
      481 ttctacagtt tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc
      541 atttaagcac acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc
      601 tgattgttga agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc
      661 tcttgcacac tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc
      721 ctaaaccaaa agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag
      781 ataaatattg gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc
      841 aactgtttac taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa
      901 gtaccgttac ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga
      961 ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt tacacgttac taaagggaat
     1021 gtagataaat tattaggtat agggcacctc taataactac caattaattc acctcaaaat
     1081 gaaattagaa aaaaacagcg aaatttttaa atctatttct tatcgataca aat
//
